LOCUS       DAXHPG010000001        51277 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXHPG010000001 DAXHPG010000000
VERSION     DAXHPG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 51277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51277
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.001"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(38..703)
                     /locus_tag="ACFM6F_000001"
     CDS             complement(38..703)
                     /locus_tag="ACFM6F_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163506.1"
                     /translation="MENIEEYALLSPEALLERLDTVLSIRIGGKGWESSMTANFAQTL
                     VEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELNFKISEGSTRVESDFKETLVRFGR
                     DMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYLKQVASEGYQTERLYNAVDRLAES
                     QERITSAILKGARGADFVQIGRRSYSREDISEANRRAERVPYGAELVSDGNFTAVLSD
                     IGD"
     gene            complement(711..908)
                     /locus_tag="ACFM6F_000002"
     CDS             complement(711..908)
                     /locus_tag="ACFM6F_000002"
                     /inference="COORDINATES: protein motif:HMM:NF019096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD1 domain-containing DNA-binding protein"
                     /protein_id="HFC6163507.1"
                     /translation="MKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALNTPYGSIR
                     TKLDGRSNPDNLVCNRYKVLL"
     gene            complement(930..1103)
                     /locus_tag="ACFM6F_000003"
                     /pseudo
     CDS             complement(930..1103)
                     /locus_tag="ACFM6F_000003"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
     gene            complement(1311..1703)
                     /locus_tag="ACFM6F_000004"
     CDS             complement(1311..1703)
                     /locus_tag="ACFM6F_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6163508.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(1820..2230)
                     /locus_tag="ACFM6F_000005"
     CDS             complement(1820..2230)
                     /locus_tag="ACFM6F_000005"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6163509.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(2244..2627)
                     /locus_tag="ACFM6F_000006"
     CDS             complement(2244..2627)
                     /locus_tag="ACFM6F_000006"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6163510.1"
                     /translation="MPKSLSLQDVQLRFSSKFPDKTVLKFTKNYEPVTIQCPLHGSVV
                     YGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGEIYEFASTLAASKFIGCSN
                     STLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(3092..4390)
                     /locus_tag="ACFM6F_000007"
     CDS             complement(3092..4390)
                     /locus_tag="ACFM6F_000007"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC6163511.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAILAYYNVQLQHLHNAEPVKAEDVMAVIEKVALRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMEGWKESTFGIKNYEE
                     LPDNAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(4494..5645)
                     /locus_tag="ACFM6F_000008"
     CDS             complement(4494..5645)
                     /locus_tag="ACFM6F_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC6163512.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTSERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            5980..7359
                     /gene="norM"
                     /locus_tag="ACFM6F_000009"
     CDS             5980..7359
                     /gene="norM"
                     /locus_tag="ACFM6F_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC6163513.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            7537..8577
                     /gene="murB"
                     /locus_tag="ACFM6F_000010"
     CDS             7537..8577
                     /gene="murB"
                     /locus_tag="ACFM6F_000010"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC6163514.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            8589..9239
                     /locus_tag="ACFM6F_000011"
     CDS             8589..9239
                     /locus_tag="ACFM6F_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC6163515.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MITKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            9294..10070
                     /locus_tag="ACFM6F_000012"
     CDS             9294..10070
                     /locus_tag="ACFM6F_000012"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6163516.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPALDFAPDDSVGKIIRTVCDNGLSL"
     gene            10121..10687
                     /locus_tag="ACFM6F_000013"
     CDS             10121..10687
                     /locus_tag="ACFM6F_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC6163517.1"
                     /translation="MSAFLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            10707..11597
                     /locus_tag="ACFM6F_000014"
     CDS             10707..11597
                     /locus_tag="ACFM6F_000014"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC6163518.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIRESKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            11622..12380
                     /locus_tag="ACFM6F_000015"
     CDS             11622..12380
                     /locus_tag="ACFM6F_000015"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6163519.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(12435..13100)
                     /locus_tag="ACFM6F_000016"
     CDS             complement(12435..13100)
                     /locus_tag="ACFM6F_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6163520.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYRAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSRQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(13301..14074)
                     /gene="folE2"
                     /locus_tag="ACFM6F_000017"
     CDS             complement(13301..14074)
                     /gene="folE2"
                     /locus_tag="ACFM6F_000017"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC6163521.1"
                     /translation="MNTIADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHTEALDFAQLRRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVSLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTADAEVGIEEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            14266..15423
                     /locus_tag="ACFM6F_000018"
     CDS             14266..15423
                     /locus_tag="ACFM6F_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC6163522.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPMDCWLVLRGIKTLALRMNAHYQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSVGIEDVDDIWNDISAALDTTL"
     gene            complement(15486..16487)
                     /gene="hemB"
                     /locus_tag="ACFM6F_000019"
     CDS             complement(15486..16487)
                     /gene="hemB"
                     /locus_tag="ACFM6F_000019"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC6163523.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMQGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTLIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGDKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(16670..16954)
                     /gene="yhbY"
                     /locus_tag="ACFM6F_000020"
     CDS             complement(16670..16954)
                     /gene="yhbY"
                     /locus_tag="ACFM6F_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC6163524.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            17064..17684
                     /locus_tag="ACFM6F_000021"
     CDS             17064..17684
                     /locus_tag="ACFM6F_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC6163525.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            17748..19715
                     /gene="ftsH"
                     /locus_tag="ACFM6F_000022"
     CDS             17748..19715
                     /gene="ftsH"
                     /locus_tag="ACFM6F_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC6163526.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQIA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            19937..20368
                     /locus_tag="ACFM6F_000023"
     CDS             19937..20368
                     /locus_tag="ACFM6F_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC6163527.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            20361..20639
                     /locus_tag="ACFM6F_000024"
     CDS             20361..20639
                     /locus_tag="ACFM6F_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC6163528.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            20692..21270
                     /gene="pth"
                     /locus_tag="ACFM6F_000025"
     CDS             20692..21270
                     /gene="pth"
                     /locus_tag="ACFM6F_000025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC6163529.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            21331..21537
                     /locus_tag="ACFM6F_000026"
     CDS             21331..21537
                     /locus_tag="ACFM6F_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC6163530.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            21534..21896
                     /locus_tag="ACFM6F_000027"
     CDS             21534..21896
                     /locus_tag="ACFM6F_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC6163531.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            22127..23542
                     /locus_tag="ACFM6F_000028"
     CDS             22127..23542
                     /locus_tag="ACFM6F_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFC6163532.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            23661..24170
                     /locus_tag="ACFM6F_000029"
     CDS             23661..24170
                     /locus_tag="ACFM6F_000029"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6163533.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            24333..25715
                     /locus_tag="ACFM6F_000030"
     CDS             24333..25715
                     /locus_tag="ACFM6F_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC6163534.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMNIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSSMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(25735..26490)
                     /locus_tag="ACFM6F_000031"
     CDS             complement(25735..26490)
                     /locus_tag="ACFM6F_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6163535.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(26500..27216)
                     /locus_tag="ACFM6F_000032"
     CDS             complement(26500..27216)
                     /locus_tag="ACFM6F_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6163536.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMVIAVAVALVRIMPAGGIARKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(27206..28033)
                     /locus_tag="ACFM6F_000033"
     CDS             complement(27206..28033)
                     /locus_tag="ACFM6F_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6163537.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            28318..29226
                     /locus_tag="ACFM6F_000034"
     CDS             28318..29226
                     /locus_tag="ACFM6F_000034"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC6163538.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            29306..32920
                     /gene="recB"
                     /locus_tag="ACFM6F_000035"
     CDS             29306..32920
                     /gene="recB"
                     /locus_tag="ACFM6F_000035"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC6163539.1"
                     /translation="MSAPIQAFDPLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAALKKGKTPDAAAFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAGDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            32959..33318
                     /locus_tag="ACFM6F_000036"
     CDS             32959..33318
                     /locus_tag="ACFM6F_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6163540.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            33560..34039
                     /locus_tag="ACFM6F_000037"
     CDS             33560..34039
                     /locus_tag="ACFM6F_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC6163541.1"
                     /translation="MKHILPLIAASALCISTASAHPAGKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMNIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(34110..35489)
                     /gene="radA"
                     /locus_tag="ACFM6F_000038"
     CDS             complement(34110..35489)
                     /gene="radA"
                     /locus_tag="ACFM6F_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC6163542.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSSVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICREDRD"
     gene            complement(35678..36742)
                     /gene="hemE"
                     /locus_tag="ACFM6F_000039"
     CDS             complement(35678..36742)
                     /gene="hemE"
                     /locus_tag="ACFM6F_000039"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC6163543.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEDDIAKLHVPDMEKLQYVFDAVSSIRKALDGRVPLIGFSGSPFTLACYMVEGGSS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDEAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGQGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(36817..38034)
                     /locus_tag="ACFM6F_000040"
     CDS             complement(36817..38034)
                     /locus_tag="ACFM6F_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC6163544.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETC"
     gene            complement(38031..39305)
                     /locus_tag="ACFM6F_000041"
     CDS             complement(38031..39305)
                     /locus_tag="ACFM6F_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC6163545.1"
                     /translation="MPSETSSPRKENETEVHIPAAPFIVKQSGSNALAVCALVLAALG
                     LGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDRLD
                     GNVKANGEQILEMQKSYRELTKGRADWLVDETETILNLAAQQLVLTGNIQTAVGVLEH
                     IDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLEIAVSGLPLILDG
                     VLKPSVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENL
                     RLRLLDARTALMQRNSEVYQGDLNNAETAVRQYFDAKSPATQSWLKELAELKALDVRM
                     TADDGLKNSLNAVRAYRDGTRMTTAENQEAEQATSEPANENTASEPAAASDVKTIEAP
                     SLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            complement(39380..40141)
                     /locus_tag="ACFM6F_000042"
     CDS             complement(39380..40141)
                     /locus_tag="ACFM6F_000042"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC6163546.1"
                     /translation="METAKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKIHIAVGQGSRRALERYLGRTV
                     IAPHDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEMAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(40159..40581)
                     /locus_tag="ACFM6F_000043"
     CDS             complement(40159..40581)
                     /locus_tag="ACFM6F_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC6163547.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLSGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            complement(40832..41152)
                     /locus_tag="ACFM6F_000044"
     CDS             complement(40832..41152)
                     /locus_tag="ACFM6F_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC6163548.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(41175..41801)
                     /gene="upp"
                     /locus_tag="ACFM6F_000045"
     CDS             complement(41175..41801)
                     /gene="upp"
                     /locus_tag="ACFM6F_000045"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC6163549.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(41907..42218)
                     /gene="grxD"
                     /locus_tag="ACFM6F_000046"
     CDS             complement(41907..42218)
                     /gene="grxD"
                     /locus_tag="ACFM6F_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC6163550.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(42319..42534)
                     /locus_tag="ACFM6F_000047"
     CDS             complement(42319..42534)
                     /locus_tag="ACFM6F_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC6163551.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            complement(42560..43339)
                     /locus_tag="ACFM6F_000048"
     CDS             complement(42560..43339)
                     /locus_tag="ACFM6F_000048"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6163552.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQVLAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDTAFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRYTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            complement(43344..43694)
                     /locus_tag="ACFM6F_000049"
     CDS             complement(43344..43694)
                     /locus_tag="ACFM6F_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC6163553.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(43698..44675)
                     /locus_tag="ACFM6F_000050"
     CDS             complement(43698..44675)
                     /locus_tag="ACFM6F_000050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC6163554.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHGEALAYLRDRGVAEPEEFLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLDVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(44709..45839)
                     /locus_tag="ACFM6F_000051"
     CDS             complement(44709..45839)
                     /locus_tag="ACFM6F_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6163555.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            46007..46708
                     /locus_tag="ACFM6F_000052"
     CDS             46007..46708
                     /locus_tag="ACFM6F_000052"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC6163556.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            46851..48644
                     /gene="lepA"
                     /locus_tag="ACFM6F_000053"
     CDS             46851..48644
                     /gene="lepA"
                     /locus_tag="ACFM6F_000053"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC6163557.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            48787..49806
                     /gene="lepB"
                     /locus_tag="ACFM6F_000054"
     CDS             48787..49806
                     /gene="lepB"
                     /locus_tag="ACFM6F_000054"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC6163558.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(50025..50174)
                     /locus_tag="ACFM6F_000055"
     CDS             complement(50025..50174)
                     /locus_tag="ACFM6F_000055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163559.1"
                     /translation="MNWQAVKTGKTKHASNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(50220..51125)
                     /locus_tag="ACFM6F_000056"
     CDS             complement(50220..51125)
                     /locus_tag="ACFM6F_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6163560.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEIETVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
BASE COUNT        12178 a        14608 c        12949 g        11542 t
ORIGIN      
        1 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atcccctata tcagataaaa
       61 cagcggtaaa attgccgtct gaaaccaact ctgcgccata cgggacacgc tctgcacgtc
      121 tatttgcctc cgatatatcc tccctactgt aggaacgtct gccgatttgc acgaaatcgg
      181 cacctctagc acccttcagg atggcggacg ttatccgttc ttgggattct gcaagacggt
      241 cgacggcatt atacagacgc tcggtctgat acccttccga agcaacctgc ttcaggtatt
      301 ccaaataacc gtaacccaac gcccctccga caagcagggt cgtcaacgct accagcgttg
      361 ccgaacggat tttagggggc atatcttgaa gcatatctct accgaaccga accaaagtct
      421 ctttaaagtc ggactctaca cgcgtagaac cctcggaaat cttgaagttc aactcatact
      481 gcttccgttt gtccgctgtc aaccgcttga ggttgtcatc cccgtattgg acggttgaca
      541 caaccctgta cagactgtcc tgtatttcga ccagcgtctg tgcaaagttg gcggtcatac
      601 tggattccca acccttgccg ccgattctga tactcaaaac ggtatccagg cgttccagca
      661 gggcttcggg agacagcagt gcgtattctt caatattttc cataggttga ttatagcaga
      721 accttatacc tgttacagac aaggttgtcg ggatttgaac gtccgtcgag cttggttcgt
      781 atagagccgt agggggtgtt tagggctttg gcggcagctt gtacgctggg gaacgtgagt
      841 gtttcgcctg tggcggtgtc gaggatggag acaggttttt ggttttgggg aggcgtattt
      901 tccttcattt taagtttccc gcactccgga cacccatact ttgactttat agctgtgtcg
      961 taatacaaaa gttggacaac cccgtgcgta ggacatctaa cactggaagg tttataaatg
     1021 ccactgaact ccaacaattc taaatgtgga aatttctctt gaaagcgtga tttggcggta
     1081 tcgaaagtca ttcgcttacc cataatttgt ctccataggt tatgtgcaaa tggattacac
     1141 aaccaaaata tagaaatttt caaatttttt gagacctacc tggaatcctg cgcaagcaca
     1201 atgtaaagaa aaatggtata cgtctttact aaatctttac ataaattaac tctaatcggg
     1261 atttgaacgt ccgtcgagct tggttcgtag gatttagggt gttgtgaacc ttacctatcg
     1321 tacccaagca ctttgtacct gtttgaaata aggttgtcgg gcgacgttcg acccttgagc
     1381 ctgtgattga tttgttggaa atgtacacct aaagcagcac ctgcggcagt aacgcttggg
     1441 aacgtgagtg tttcgttggt cgttgtgtcg aggatgagga ggggcttccc tacatttgga
     1501 ggtgtttggg tttttgacat cttagcacac tcaggacacc cgtactttga ctttataagg
     1561 gcattaaatg tcgaataacg aacgttcccg tgcataggac aattaacggt tactgggtaa
     1621 ttcacaccgt caaagccggt tagagagata ttagggaata tatcagtaag ttcagaagct
     1681 gcttggttga aagatttggc catttgagac tccaaaattg attgtcaaat ggatttaata
     1741 gcaaatttat aaaaatgtca aatttttcga gaagcacctg atagtggcga ggggtataca
     1801 ctgttaaaaa gtgtatacgt catttagtgc agatgtgaac cttatacctc cctgcaagta
     1861 aagtctctgt gtgaacccga ccttttatac ggtcagtgat agctgctggg tttataccca
     1921 atgaccttgc agcagatgct cttgagggga atgtaagtgt ttcgccagtt acagtatcct
     1981 ccaatactgt atttttagat aatttatcta ttgtatcttc tggaattttg tgggaattga
     2041 caccataaga tgcacatttt ggacacccac ttttagagtc caacatactt ttgaatgtag
     2101 agcaagtaac cctcccgtgt ataggacaga cgattaccgt cgggtaacga acgccattga
     2161 actccacaag attcaaatgt ggaaatttag aggttagctt agaaactgct tgagtaaatg
     2221 taagtgccat agtggttgtg aacttacttt accaacctat aacgatacct aatcaatcgg
     2281 tcaaacggag tgcgtccgct taaacgtaca gctaaagtgc tgttggagca accgataaat
     2341 ttactggcag ccagtgtact tgcaaactcg tagatttccc ctgtctctgt gtcctccaac
     2401 ctaatggcaa cggcgtttgg agagggcttt tcgggatttc gagtacattc aggacatcct
     2461 gttgaggatt tcatggcagc ttgtagattc ccgtaaacaa ccgacccatg caaagggcat
     2521 tggattgtta ccggttcgta gtttttagtg aacttcaaaa ccgtcttatc aggaaattta
     2581 ctggaaaacc ttaactgaac atcttgtagc gagagtgatt ttggcattat agacctttct
     2641 aaaatgaaag cattatacaa ccaaaatata gaaattttca aattttctct gaacgcactg
     2701 gtatccgatg gagctaaatg taaagaaaag ggtatatgtc cttactaaat ctttacacat
     2761 tttaactgta tagtgtttga caagcaacag gcataggcgc ataatgcacc cgtagttaga
     2821 tagatagtaa ggagataacc aatgacttag cacctgcccc cctctctctc gcgcaccccg
     2881 cccctcgctt gcggctaatg ggctttgcgc gaaatataca ctttcatctc ctgcatggct
     2941 taaccgccta tgcaatagta cacacatcaa caaatcaggc tggcacgtct tggcattggc
     3001 tgacgtacca ttgtagcccg aaatgttgat accctactat ttatgaccaa cggcggatta
     3061 gggcttcaga cggcaatttt actgcaaaac cttatgcgaa cggatgatgc agcacaatcg
     3121 tttcttctcg gtcggggccg gtggagacga tggcgaccgg cgcgccgcag acttcttcaa
     3181 tccgtttcaa atatgctttg gcattatcgg gcagttcctc ataatttttg atgccgaaag
     3241 tcgattcttt ccaaccctcc attgtttcgt aaatcggttt gcaggtttcc accgcatccg
     3301 aaccgcaagg caggatatcg gttttgccgc cgtcgggcaa ttcgtagccg acgcagatat
     3361 tgatggtttc aacgccgtcc attacatcga gtttggtaat gcacataccg gaaatgccgt
     3421 tgatttggat ggagcgtttc agggcggcgg catcaaacca gccgcagcgg cgtgcgcggc
     3481 cggttaccga gccgaattcg tgtccgcgtt ccgccaaacc tgcgcctact tcgtcgaaca
     3541 attcggtcgg gaacgggcct gaaccgacgc gcgtggtata ggctttgacg atgcccaaaa
     3601 cataatccag catttgaggg cctacgcccg cgcctgcgga agcggctccc gccagacagt
     3661 tggacgaggt aacgaaggga taagtgccgt agtcgatgtc caacaacgca ccttgcgcgc
     3721 cttcaaacag cagtttttcg ccgtttttgt ttttctcgtt caacacgcgg gacacgtcgg
     3781 taatcatcgg cgcaatgcgc agcgcgactt tttcgataac cgccatcacg tcttccgctt
     3841 taaccggctc ggcattgtgc agatgttgca gttggacatt gtaataggca aggatggcat
     3901 ccagtttttc acgcagtttt tcaggatgca gcaaatcggc ggcgcgaatg gcgcggcgtg
     3961 ccactttgtc ttcgtaggca gggccgatgc cgcggccggt cgtgccgatt ttgcctttgc
     4021 cgcgcgatgc ttcgcgggct tggtcgagcg cgatgtggta aggcaggatc agcgggcagg
     4081 tcggcgcgat tttcagacgg ccttcgacgt ttttcacgcc tgcagcgttt aactcgtcga
     4141 tttcgcccaa cagggcttcg ggggagacga caacgcccga accgatgaag cagtccagac
     4201 tttcatgcag gatgccgctc ggaatcaggc gcaaaatggt ttttttgccg ccgacgacca
     4261 aagtatgacc cgcattgtgg ccgccttgga agcgcaccac gccgccggct tcttccgcca
     4321 gccagtcaac gattttacct ttaccctcgt cgccccactg tgcgccgatt actacaacat
     4381 ttttagccat agccatataa cctatcgata ttaaaaatta ttgcggaaat ccccaaaccg
     4441 gtgcggcttt gcgccccacc acgatttgcc tttcatccgc catgaacggg tatttagcgt
     4501 ttcacgacct gccaaacgcc gtccgtcttt ttcagacggc ctgcaagctc ttccgaaaca
     4561 ttgtgtccga taccgtagtc aatcacgaca cactgccctt gttcacgcaa ggcttcgact
     4621 gcttcgcgcg ccgcttcggc atcttccgca tcgaccaaca cggcgggctg ccgttcgctg
     4681 gtgggcaaac gcccgataaa gctgcgcaag tcgaaactga atcccgttgc cgggcgcgcc
     4741 ctgccgaaat atccgcccaa tccgtcataa cgcccgccgc gcgcgaccgc gtcgtggaaa
     4801 tcggcggcat aggcggcata cagcaagccc gtgtggtaat tgtcgacgcg cagctcggac
     4861 aagtcgatat ggatttcaca atcggggaat gcgtcgcaca ccgcctgcaa ttcgcccaac
     4921 gcgccgccga ccgccgacaa atccggcaac cgtccgcgcg cgtcggacaa cacttcacgc
     4981 ccgccgtaca ggcgcggcag cagcgagaat gcttttgccc acataccgtc cagcttccaa
     5041 gccttgacct gcgcttcgac cgtctcggta tctttatcct gcatcaaggc aagcagcgtt
     5101 gcagactgcc ccgcatccaa atgcgccgca tcggacaagg cgcgaaatat gccgatatgc
     5161 cccagcgaaa gcagcacttt gcccatatcg gcaattttca tgcttttcag catcaggtct
     5221 atcagctcga tgtcgccacg gatgtcggca aaaccgtaca tttccgcccc tgcctgcaag
     5281 ggttcgcgca tattcagcag accgtcggac tgcgcgtgca acaccggacc ggcataacac
     5341 aaccggttaa tcccttggtt ggcggataaa agatgggcat cgatacgcgc cacctgcggc
     5401 gtgatgtcgg cgcgtatgcc caactgcctg ccgctgagcc tgtccgttac caaaatggtt
     5461 ttcagggaaa gccccgcatc gatatgcgtc agcagggaat gtgcgtactc catcagcgga
     5521 ggctgtacca gttcataacc gtgtacgcgg aacagtgcca acaactgctc cctcgcgctt
     5581 tcaagctgcc gcgcgttcgt gggcagtacg tcggcgatat gttccggaag ctgccatgtc
     5641 tgcatgagaa tctgccttct attttatcat gtcaatacaa aaggctgccc gatccgcaat
     5701 cagacggcat ctttgccgca acgcatcacg ccgctgtcaa aacggcgatg cttccttccg
     5761 attcagaatc cgaacagccc ctgtccgacg gctcaatgcc gtcaagtcgt taaaaccaaa
     5821 ctttaccata aaatacacac aatctgaata tacttttata ccctgtatcc gccgtctgac
     5881 ggcacggcat ttttttacga tataatgtgc gccattattt ctcatcactt cctgctgccg
     5941 ttttctgctt cagacggcat ttttgttgat tgaacactta tgctgctcga cctcaaccgc
     6001 ttttcctttt ccgtcttcct gaaagaagtc cgcctgctga ccgctcttgc cctgcccatg
     6061 ctgttggcgc aggtcgcgca ggtgggcatc ggttttgtcg ataccgtgat ggcgggcggt
     6121 gcgggcaagg aagacttggc ggcggtggct ttgggcagca gcgcgtttgc cacggtttat
     6181 attaccttta tgggcattat ggcggcgctg aacccgatga ttgcccagct ttacggcgcg
     6241 ggtaaaaccg acgaagtggg cgaaacggga cggcagggga tttggttcgg gctgtttttg
     6301 ggcgtgttcg gcatggtctt gatgtgggcg gcgattacgc cgttccgcaa ctggctgacc
     6361 ttgagcgatt atgtggaagg cacaatggcg cagtatatgc tgttcaccag cttggcgatg
     6421 ccggcggcaa tggtacaccg cgcactgcac gcctacgcct ccagcctgaa ccgcccgcgc
     6481 ctgattatgt tggtcagctt tgcggcgttt gtgttgaacg tgccgctgaa ctatattttc
     6541 gtttacggca aattcggtat gcccgctttg ggcggcgcag gctgcggact ggcgacgatg
     6601 gcggtgtttt ggttcagcgc gctggcattg tggatttata tcgccaagga aaatttcttc
     6661 cgcccattcg gactgacggc gaaattcggc aaaccggatt gggcggtgtt caaacagatt
     6721 tggaaaatcg gcgcacccat cgggctgtct tattttttgg aagccagcgc gttttcgttt
     6781 atcgtgtttt tgattgcgcc tttcggcgag gattatgtgg cggcgcagca ggtcggcatc
     6841 agtttgtcgg ggattctcta tatgattccg caaagcgtcg gctcggcggg gacggtgcgc
     6901 atcggctttt cgcttgggcg gcgcgaattt tcgcgggcgc gttatatttc gggcgtgtca
     6961 ctggtgtcag gatggatgct cgccgtgatt accgtgcttt ccttggtatt attccgttcg
     7021 ccgctggtaa gtatgtacaa caatgatccg gcggttttaa gcatcgccgc caccgtctta
     7081 ctgttcgccg gcttgttcca accggcagac ttcacccaat gtatcgcctc ctacgccttg
     7141 cgcggctaca aagttacaaa ggtgccgatg ttcatccacg ccgccgcctt ttggggctgc
     7201 ggtctgctgc cgggctacct gctcgcctac cgtttcgata tgggcattta cggcttctgg
     7261 acggcattga ttgcctcgct caccatcgcc gccatcgcct tggtgtggtg cttggaattg
     7321 tgcagtaggg agatggtcag atcgcataag gctgtctgaa aactccgccg attaaaaata
     7381 tagtggatta acaaaaacca gtacggcgtt acctcgcctt gccgtactat ttgtactgtc
     7441 tgcggcttcg ttgccttgtc ctgatttttg ttaatccact atagaaacga tgggctgaag
     7501 cccgccactg gcaatactca ttttaaagtt caaaccatgc aacccatccg ataccgaacc
     7561 gaccttaccc cctacaacac tttcggcctg cgcgcccaag cccgagcctt tattgcgctt
     7621 gaacacgccg acgggttgcg cgacatcgtc cggctacccg agttcaaccg agatactgtt
     7681 ttatggctgg gcggcggcag caatatcctt ttgatggagg actacgccgg tctggtcgta
     7741 catatggaaa acaaaggcat acgcgaaatt gctcgttcag acggcatggt tctgattgaa
     7801 gcgcaggcgg gcgaaatctg gcacgatttt gtcctgcacg ccgtcgcgct gggtttgaac
     7861 ggtttggaaa acctgagcct gattccgggt acggtcggcg cgtcgcccgt gcagaacatc
     7921 ggcgcatacg gcgtagaggc gaaagatgtg attcacagcg tgcgctgctt cgatttggat
     7981 acggaaacct ttgtaaccct ttccaatgcc gactgccgct tcgcctaccg cgaaagcctg
     8041 ttcaagcagg aaggtaaagg gcgttatgtg attgtttcgg tcgtatttgc attaaaaacg
     8101 cattttgtgc cgaacttggg ttacggcgat ttggcggccg ccgttgccga actgagcgcg
     8161 ggcagggtgc cgacggcgaa agatgtttcc gatgcagtgt gtgcaatccg caacagtaaa
     8221 cttcctaatc ctaacgtgct tggcaatgtc ggcagtttct ttaaaaaccc cgtcgtcagc
     8281 gcagaaaaag ccgccacctt gttgcagcgg catcctgata tgccgcgcta tccgcagccc
     8341 gacggttcgg tcaaactcgc cgccggctgg ctgatcgacc aatgtcgtct gaaaggcttt
     8401 caaatcggcg gcgcggcggt acatgacagg caggctttgg tcttagtgaa caaaaacaat
     8461 gcctcggcaa acgatgtccg gcagttggcg caacacatca aatttacagt atttgctcgg
     8521 tttcaggtag aattgcacgc cgaacccaat tggctgcctg cttcgttcag cctgtaaatc
     8581 caaggagtat gcccgtgacc cgccccgcca aaatcaatac ttacacacgc atcatcgacg
     8641 ccagccttgc gcttttcaac gaggaaggcg agcgcaacat cagcaccaac catattgccg
     8701 cccacttggg catcagtccg ggcaacctct attaccactt ccgcaacaaa gacgaaatca
     8761 tcgtccaact gttcaaacgt tacagcgaag ccctgctgga atacctcaac gaagccgtgt
     8821 tgccgtctga tgtggaagac tccatcaatt atatggcggg catttacgac gtgatgtggg
     8881 aataccgctt cctattcagc gacgtgaaca ccctgcttgc acgcagtgcc gaactattgg
     8941 gcgaacacaa taccttcacc caagccaaag tctccccgct cttggtcaac ctgctgaccc
     9001 agctcaacgg tctgaacatc atccaagccg accaaaccgc catgaacgac ctcgccgtca
     9061 atatgtggat gatcacgaaa tactggttcg acttcgacag ctccctgcgc ggccgcacca
     9121 agctgaccga agactccaaa gcacgcggcg tccgtcgtac cttaagcctc cttcgtcctt
     9181 atcttttgcc cgaacaccgc gagaaatacg accggaaaat cggcaacggc aacccgtaaa
     9241 cccacagcgt ccgaatcagc cttcagacgg cacttacccg acggtatcag cgaatgcgcg
     9301 cagtcccccc gccccatatc tccatcacag gcctaggtta cctcggcctg ccgctggcac
     9361 aaaagtttta ccaacacggc agccgcgtcg ccgccataaa acgcagcctg acttcggacg
     9421 acatcaacct gcccatacat ctcgacacct tcgacctcgg cagcaccgat gcctttcaga
     9481 cggcattgtg gcggcaccac gccgacaaac ccgtctggtt cttcctgcta ccgccctcct
     9541 cgctgacgca ttacgccgac accgtcaaac aatgggcaga acttgcccgg gcgtgcaacg
     9601 tgcaacacct gattttcaca agcagcacca gcgtttacgg cgatacagcg cgcgaatgcg
     9661 acgaaaccgc cacacccgat ccgcaaaccg agtccgcccg ccaaatcctc gccgccgaac
     9721 aacacctgct cgacagcggc atctccaaca tcgacatcct gcggctgggc gggctttatt
     9781 gcgccgaacg ccatcccgtc agccgccttg ttcaaaagca aaacatcccg ggcggcaacc
     9841 gccccatcaa catcgtccac cgtaatatcg ccgtcgaaag cctgtttcag acggcattta
     9901 accccggcgg caggcggctg aaaaacatta tcgaaccgcg ccacccgaca cgacgcgaat
     9961 tctacacgga agaagccgcc aaactcggcc tgcccgcgct ggatttcgca cccgacgaca
    10021 gcgtgggcaa aatcatccgt accgtttgcg ataacggctt aagcctgtaa aataagcggc
    10081 aacagcaaca aacaggcatt ctccgcccca atcagaaaat atgtccgcct tcctccccat
    10141 catcaaccgc ctgattctgc aaagcccgga cagccgctcg gaacttgccg cctttgcagg
    10201 caaaacactg accctgaaca ttgccgggct gaaactggcg ggacgcatca cggaagacgg
    10261 tttgctctcg gcgggaaacg gctttgcaga caccgaaatt accttccgca acagcgcggt
    10321 acagaaaatc ctccaaggcg gcgaacccgg ggcgggcgac atcgggctcg aaggcgacct
    10381 catcctcggc atcgcggtac tgtccctgct cggcagcctg cgttcccgcg catcggacga
    10441 attggcacgg attttcggca cgcaggcaga catcggcagc cgtgccgccg acatcggaca
    10501 cggcatcaaa caaatcggca ggaacatcgc cgaacaaatc ggcggatttt cccgcgaatc
    10561 cgagtccgca aacatcggca acgaagccct tgccgactgc ctcgacgaaa taagcagact
    10621 gcgcgacggc gtggaacgcc tcaacgaacg cctcgaccgg ctcgaacgcg acatttggat
    10681 agactaacct tcagacggca tccgacatga acagcccttt tcacaacatc ggcatcgtaa
    10741 cccgccccaa cacgcccgac atccaagaca ccgcacacac gctgattacc tttttgaagc
    10801 agcacggctt taccgtctat ctcgacgaag tcggcataaa ggaaggctgc atctataccc
    10861 aagacaccgt cggctgccat atcgtcaaca agaccgaact ggggcaatac tgcgacctgg
    10921 tcgccgtttt aggcggagac ggcacctttc tctccgtcgc ccgcgaaatc gccctgcgcg
    10981 ccgttccgat tatcggcatc aaccaagggc atttgggctt cctgacccaa attccccgcg
    11041 aatatatgac ggacaagcta ttgcccgttt tagaagggaa ataccttgcc gaagagcgca
    11101 tcctgattga ggccgcactc atccgcgaaa gcaaaaccgc cgaacgcgcc atcgccctca
    11161 acgatgccgt cctctcccgt ggcggtgccg gacagatgat tgagtttgaa gtcttcgtca
    11221 atcgggaatt cgtctatacc cagcgttcgg acgggctgat tgtctccacc cccaccggat
    11281 cgaccgccta ttcgcttgcc gccggcggcc ccatcatgca ggcaggatta cacgccttca
    11341 cgctcgtccc catctgcccg caatccatga ccaaccgccc catcgccatt ccagacacgt
    11401 ccgaaatcga aatcctcgtt acccaaggcg gcgacgcgcg cgtccatttc gacggtcaaa
    11461 cccatatcga cgtgcaaaac ctcgaccgca tcaccatccg ccgctaccga aaccccttac
    11521 gcatcctaca tccgaccgac taccaatatt tcaaaaccct gcgccaaaaa ctgcactggg
    11581 gcgaacaatt ggtctgaacc gacccttacc ggaaaacacc tatgaatcca aaaactcccc
    11641 ccgaccatac agaagatgcc gtgcgcctct ccaaacgcat ggcgcaactg gggctttgtt
    11701 cgcgccgcga agccgacggc tacatcgaac agggctgggt aacggtcaac ggcaaaaccg
    11761 ccgtacttgg tcagaaagtt tcaccagcag accgtatcga actgaacaag aaagcccacg
    11821 aacagcaggc ggcacgcatt accatcctgt tgaacaaacc cgtcggctat gtcagcgcac
    11881 aagcggaaaa aggctataaa tccgccgccg aactgattac ccctgaaaat cactgggaag
    11941 gcgataccag ccgcatccgt ttcgatccga aacacaaaat cggtcttgcc cccgccggca
    12001 gactcgatat cgattcagtc ggattgctgg tgttgacgca ggacggccgt atcgccaagc
    12061 agcttatcgg cgaaaacagc ggcagtgaaa aagaatattt ggtgcgcgtg cgcggaaaat
    12121 tggacgaaaa aggacttgcc ttactgaatc acggattgag tttggacggc gagaaactgc
    12181 gtcccgccca agtagaatgg caaaacgaag accaactgcg cttcgtgttg aaacagggta
    12241 aaaagcggca aatccgccgt atgtgcgaac tggtcgggct gcgcgtcgtc gggctgaaac
    12301 gcatccgcat gggcaaggtc aaactcggca ggctgccgcc cggcaaatgg cgttatctcg
    12361 cccccggcga atcgttttaa ataagcatta aaaaatgccg tctgaaaatc cttgcgtttt
    12421 cagacggcat tgccttatgc ccaaataacg gtttcttcca agggcctgcg cgccttcgtg
    12481 gcgatttcct gaacgcggta gccgaatgtc gcggcgacgg acacgcccca ttctgccgca
    12541 tcgaacaaac cgaactgcct ggacaatacg cgttccatat cggcatagtt gaaaccttcc
    12601 accggacagg aatcgatacc tgccatcgcc gcacccgtca tcatgttgcc caacgcgata
    12661 taggtctggc ggcaacacca gtcaaacaag gcgcgcgaat cgtccaaaat cttgatgtcg
    12721 tcggcttgaa acgcccgata cctcgccaaa gattttgcca tggcatccgg ttcggtaacg
    12781 ccgcgccgtt tgaggctttc caacataaac gggctgtcga agcgggcatt tttctttgcc
    12841 aaaaacacca ccaaatgact ggcggtatcc aaagcatccg ccatacccca agaaaacggc
    12901 ttgattgcct gtcggatttc ggggttttga accacaacaa actgccaagg ctccgaaccg
    12961 accgaactgg gcgacaaacg cccgagttct aaaataaact gaaaatcctc ggcactgatt
    13021 ttacgtgccg catcgtaatg ccggcatgat ttgcggtttt taaatgcgga taggacctgc
    13081 tctttgctta atactgtcat cgtgcatcct tttattactt tgattgcaaa ttgatttcaa
    13141 accgatacgg ctctgccgca ccatacaatc cgtcctcgcc atcaagcttc atccggactt
    13201 tacccatctc tgctacggct gggaaaacaa agcggcggca gactcgggaa taacgcccca
    13261 atgcctttca gacggcattt gggcatcatt gcaaacgcgc ctacgggtag gcgatatagg
    13321 cataagccga atggttgtgg atagactcga aattctcgct ctcgacaacg aaactcttga
    13381 tgcgtttgtc ggcgatcagc gaagtagcga catcgcgcac catatcttcc acgaatttcg
    13441 ggttttcgta ggccttttcg gtaacgtatt tttcatcggg gcgtttgagc aggccgtaga
    13501 gttggcagct cgcctgcgcc tccacataat cgatgacttc ctcgataccg acttcggcat
    13561 cggcagtcag gctgacggta acgtgcgaac gctggttgtg cgcgccatat tgggaaattt
    13621 ctttggaaca cgggcaaagc gaggttacgg gaatcatgac cttcatactg tggccgtatg
    13681 ccccgtcttt gatttcgccc gtgaggctga catcataatc cagtaaggac cgaataccgg
    13741 aaaccggggc ggttttcttg cggaaaaatg ggaaagaaac gctgattttg ccggcgcggg
    13801 aatccaaaag cgcgaccatt tcagcagtca gcctgcgcaa ttgtgcaaaa tccaaggctt
    13861 cggtatgttg ctccatcaat gcgacaaagc gcgacatatg cgtacccttc tgctcggcag
    13921 gcaggtaaac cgtcatggtc agacgggcaa ctgtggactg gatgccttct gcagtttgca
    13981 gggtaatcgg aaagcgcagg tctttgatac cgacctgatt gatcggcaga ttgcgcaaat
    14041 ctcggctgga ttgcacgtct gcaatagtgt tcatgagttg tttttcctta atattcatgt
    14101 aattgttgag atgtgtgcat ggtatggcgt ttccaccggg gaatataccg tcaatctgca
    14161 aatgcgagat tatatcactc gcttttagca gctgcattga ttgtttctat ttttcagata
    14221 gtgataaaat cttcgattat tcacatctac ataggcagag accaaatgaa attcgccacg
    14281 aaagccattc attccagcta cgattgcgac gaacacaacc gcgcgctgat gccgccgatt
    14341 tatcaaaaca gtatgttcgc gttgcacgag attggcgaaa atgtgcctta ccgctactcg
    14401 cgcctgagca acccgacccg tcagatttta gaagacaccg ttgccgattt ggaacacggt
    14461 gcggcaggtt ttgcgttttc cagcggtatg gcggggattg atgccgtgtg gcgtaccttc
    14521 ctacaaccgg gcgataccat cgtcgcggtc gccgatattt acggcggcgc gtatgattta
    14581 ttggtcgatg tttatcaaaa atggggggtg aacgttgttt ttgccgattt gggcaatccg
    14641 gataatttgg acgaattgct caaagcgcac aaggtcaaac tggtttggct ggaaacgccg
    14701 tccaatccgc ttttgcgctt ggtagacatc aaagcgcttg ccgcgaaagc caaagcggcc
    14761 ggcgcactgg tcggtatcga caacaccttt gccacgccgt atctgcaaca gcccttggat
    14821 atgggttgcg attttgtgtt ccattccgct accaaatatt tgtgcggcca ttccgacgtg
    14881 ttgatgggca tcgtcgttgc caaaaccaaa gagctggcgc agcctttgca cgatatgatg
    14941 gtgcataccg gcgcggttgc cggcccgatg gactgctggc tggtgttgcg cggcatcaaa
    15001 acactggctc tgcgcatgaa cgcccattac caaaacgcac tcgaaatcgc gcgccgtttg
    15061 gaagcccatc ctgccattga aaaagtgttc catcccggcc tgccgtctca cgaacattac
    15121 gaactggcga aaacacaaat gcccaaaggc ataggcggcg tggttacggt ttatctcaaa
    15181 aacgacacgc gtgaagcggc aaacagcgtg attaaaaaca tgaaactggt caaaatggct
    15241 tccagcctcg gcggcgtgga aagtttggtc aaccattgct attcccagtc ccacagcggc
    15301 gtgccgcata atgtgaaaat ggaaatgggc atcagggtcg gcctgctgcg tttctctgtc
    15361 ggtatcgaag atgtagatga tatttggaat gatatttccg ccgcactgga tacgactttg
    15421 taaaccgtaa aaatgccgtc tgaaaccatg gtttcagacg gcatttcaat taacccggcc
    15481 gaaaatcaac gcttcagcat cttggccgcc tcaatggcat aataggtcaa aatcccatcc
    15541 gcgcccgcac gtttgaatgc cagcaggctt tccaaaacca ctttatcgcc atccagccat
    15601 ccgttggcaa ccgccgcctg tagcatcgca tattcgcccg aaacctgata ggcataagtc
    15661 ggcacaccga actcgtcctt cacgcggcgg acaacgtcca aatacggcaa accaggcttg
    15721 accattacca tatccgcacc ttcctgaatg tccaacgcca cttcgtgcaa cgcctcatcg
    15781 gtatttgccg gatccatctg gtaggttttc ttgtccgcct tgcccaaatt gcccgaactg
    15841 cccaccgcat cacggaaagg gccgtaaaat gcagaagcat atttggcgga atacgccata
    15901 atcagcgtat ggatatgccc ggcatcctcc aacgcctcgc gaatcgcacc gatacgcccg
    15961 tccatcatat cggaaggagc aaccacctgt gcgcccgctt cagcgtggca caaagcctgc
    16021 ttgaccaaaa cctctacggt ttcatcgttc atcacataac cgttttcgtc cgtcagcccg
    16081 tcctgaccgt gaaccgtata aggatcgagc gcgacatccg tcataatgcc cagttcagga
    16141 aacctctcgc gcaaggctcg gacagttgac ggcacgagtc cttcgggatt gtacgcctcc
    16201 tgcgcacgct cagttttgtt tgccgtaacc acgggaaaga gtgccaacat cggaataccg
    16261 agctttaccg cctcttccgc cgtaaacagc agcctgtcca aactctgacg cttcacgccc
    16321 tgcatagaag gcacatcctc ctcgcgcgcc gacccctcca atacgaacac cggataaatc
    16381 aaatcatcgg cggtcagcgt gtgttcgcgc atcaggcggc gtgaaaaatc gtccctgcgc
    16441 atacggcgca tacgcgaagc cggaacattg cggtaaggaa actgcataag ccctccaatc
    16501 atcctgtcaa caattcaaac catacggaag ccgccgcaac ttgcagacag catcgggcgg
    16561 caatcaaaat ccgttttcac cggaaagggt ttcggcccgc cgaaatgccg cgccgaagga
    16621 cggcgtcccg cccattaaag aacaatagca aaacaacagc ttcaggctgt cagccttcga
    16681 tattcttacg ccacaatacc aaaagtttcc cgatatgctg aacaggttgc gcgtcaaccg
    16741 cctcacataa ggcagtgcag atttcgatac gttcggcacg gtcgtcgccg aatacgcgca
    16801 ctttaatcag ctcatgcgcc gtcaatgccg catcggtttc cttgatgacc gcgtccgtca
    16861 gaccctgctg accgaccatc acaacaggat ggagatggtg cgcgcgcgct ttcagttcca
    16921 aaatttcttt ggtgttcaat ttggtatccg tcattttcct actgcttgaa taaaaagaat
    16981 aatgcggcat tgtacgcgat ttggcacgac atcggcaaaa ttaacgtaca atacgcggtt
    17041 tccattccga cccgcgaacc actatggctg tacgttccaa atcctcaaaa gcgtggctgc
    17101 acgaacacgt caacgaccac tacgtccata tggcgcaaaa agacggctac cgcgcccgtg
    17161 ccgcatacaa acttttggaa atcaacgaaa aagacaaatt aatcaaaccc ggcacggtac
    17221 ttgccgactt gggcagcgca ccgggaagct ggtcgcaggt tgccgccaag ctgacgggta
    17281 cttccggagc agttttcgcc ttggacatcc tgcctatgga agccataggg ggcgtctcct
    17341 tcattcaggg cgacttccgc gagaacgacg tactggcaca attcgaaacc ttgttggaca
    17401 accgcccgct cgaccttgta atttgcgata tggcgcccaa tatgtcggga aacgccgtaa
    17461 gcgatcaggc acgcagcttt tatctgtgcg aactggcttt agacttcgcc tcgcaacacc
    17521 tgaaaaccgg cggcagcttt ttggtcaaag tctttcaggg tgcaggctat caggaataca
    17581 tggcagccat gcgcgaaatt ttcggcacgg tgcagacgcg caaacccgaa gcctcgcgca
    17641 atcgctccag tgagatttat ttattgggca aaaataaacg ctgacaatac agacggcgtg
    17701 ctttacaatc atttccgttt tacacctcaa ttatggagcc ttgctaagtg gggaacacct
    17761 ttaaatcaat ccttgtctgg gtcgccttgg gtatcggcct gatggctgcg ttcaacgctt
    17821 tagacggtaa aaaagaagac aacgggcaaa tcgaatattc tcagttcatc caacaggtca
    17881 acaacggcga agtatccggc gtcaacatcg aaggatccgt cgtcagcggc tacctgatta
    17941 agggcgagcg caccgacaaa agcaccttct tcaccaacgc gcctttggac gacaacctga
    18001 ttaaaacact gctcgacaaa aacgtccgtg taaaagtaac gccggaagaa aaaccgagcg
    18061 cgctggctgc cctgttttac agcctgctgc ccgtcctgct gctgattggc gcgtggttct
    18121 actttatgcg tatgcagacg ggcggcggcg gaaaaggcgg cgcattctca ttcggcaaaa
    18181 gccgcgcacg cctgctggac aaagatgcca acaaagtgac ctttgccgat gtcgccggct
    18241 gcgacgaagc caaagaagaa gtgcaggaaa tcgtcgatta cctcaaagca ccgaaccgct
    18301 atcaaagctt gggcgggcgc gtgccgcgcg gcatcctgct ggcgggcagc ccgggtacgg
    18361 gtaagacgct tttggcaaaa gccattgcag gcgaagccgg cgtgccgttc ttcagcattt
    18421 caggttccga ctttgtcgaa atgttcgtcg gtgtcggtgc gagccgcgtc cgcgatatgt
    18481 tcgagcaggc gaagaaaaac gccccctgca tcatctttat cgacgagatt gacgcagtcg
    18541 gccgccaacg cggcgcaggt ttgggcggcg gcaatgatga gcgcgagcaa acattaaacc
    18601 aattgttggt tgaaatggac ggttttgaga gcaatcagac tgtaattgtg attgcggcaa
    18661 ccaaccgccc cgacgtactc gatcctgcgc tgcaacgccc cggccgtttc gaccgccaag
    18721 tcgtcgtccc cctgccggac atccgggggc gcgaacagat tttgaacgtc cattctaaaa
    18781 aagtgccttt ggacgaatct gtggatttat tgtccctcgc gcgcggcacg ccgggttttt
    18841 ccggtgcgga tttggcgaac ttggtcaacg aagccgccct gtttgccggc cgccgcaata
    18901 aagtcaaagt cgatcagagc gattttgaag acgccaaaga caaaatctat atgggtccgg
    18961 aacgccgcag tatggtgatg cacgaagacg aaaaacgtgc gacggcgtat cacgaatccg
    19021 gacacgcgat tgttgccgaa agcctgccct ttaccgaccc cgtccacaaa gtaaccatta
    19081 tgccgcgcgg acgtgcgctg ggtctgactt ggcagcttcc ggagcgcgac cgcatcagta
    19141 tgtataaaga tcagatgttg agccagctct ccatcctgtt cggcggacgg attgccgaag
    19201 acatcttcgt cggacgcatc tccaccggcg catcaaacga ctttgaacgc gcaacccaaa
    19261 tggcgcgcga gatggtaacg cgctacggca tgagcgacaa aatgggcgtg atggtttatg
    19321 cggaaaacga aggcgaagtc ttcttgggac gcagcgtaac ccgttcgcaa aacatttccg
    19381 agaaaaccca gcaggacatc gacgcggaaa tccgccggat tttggacgag cagtatcaga
    19441 ttgcctacaa aatcctcgat gaaaaccgcg acaagatgga aacgatgtgc aaagccctga
    19501 tggaatggga aaccatcgac cgcgatcagg tactggaaat catggcgggc aaacaaccca
    19561 gcccgcccaa agattacagc cacaacctgc gcgagaatgc ggacgcggcg gaagataacg
    19621 cgccgcacgc tccgactcgg gaagaaaccg aagcacctgc cccggcagac accgcttcga
    19681 cagagtccga gcagcagcct gaaaacaagg cttaacttcc cgaacaaacg gcagcccgca
    19741 agctgccgtt ttcccatccc gttttcagac ggcattttcc actcaatgcc gtctgaaacg
    19801 ccaaagccgt atcggttttt acgaatctgc gatatggcgg tttaaattga aatcagaacg
    19861 gggcaaggct gtaccggttt aaaattaaac cgctataata ccgcccttcg aatacacgcc
    19921 cgcattggtg atgcccgtga aaaaagttga aaaaaacatc ttggtgctgc acggcgcgga
    19981 caaaatgttc gagctggtcg acaaggttga ggattacccg cactttctgc cgtggtacag
    20041 caagaccgaa gtcatcgggc gtagcggcaa cgaactgaag gcgcggctgt ttatggatta
    20101 tatgcacgtt cgccaatcgt ttgccacgca caaccgcaac attccgggca gggaaatccg
    20161 tatggaactg ctcgaaggtc cgttcaaaac cttacgtgga acgtggaaat ttatcgattt
    20221 gggcgacgat atgtgcaaaa tcgaattcaa tttggaatac gatttttcca atgccgtttt
    20281 gtccgcctta atttcccccg tcttcaacca cctttccgcc acgctggtcg aagcgttcgt
    20341 caaagaggca gaccgccgtt atgcttgaaa ttgagattgt gtacgggctg cccgaccgac
    20401 aggttttgaa aaccatgcag cttgccgagg gaacaaccgt ccgcgccgcc gcactgcaaa
    20461 gcggtttgga cggcatattt gaagatttaa acctgcattc cgcgcctttg ggcattttcg
    20521 gcaaagccgt caaagacgac acgcccctgc gcgacggcga ccgcatcgaa gtgtaccgcc
    20581 cgctgttgat cgaccccaaa gaagcgcgcc gcaaacgcgt tcaaaatcaa gaagaataac
    20641 catgccgtct gaagccttca gacagcattc gacagaaaca cggaaaatat catgtcaaac
    20701 aaaatcaaaa tgcttgtcgg attgggcaac cccggcaaag aatacgaaca aacccgacac
    20761 aatgcgggtt tttggttcct cgacgaactg gcgtggaaat ggaaggcttc atttaaagaa
    20821 gaaaaaaaat tcttcggcga agtcgcccgt gccgccctgc ccgacggcga cgtttggctg
    20881 ctcaaacctg ccacgttcat gaaccgttcc ggacaggcag ttgccgcgct tgcacagttc
    20941 tacaaaatca aacccgaaga aatcctcgtc gtccacgacg aactcgacat tccctgcgga
    21001 cggattaaat tcaaactcgg cggcggcaac ggcggacaca acggcttgaa agacattcag
    21061 gcaaaactcg gcacggcaga ctattaccgc ctgcgcctcg gtatcgacca cccgggcgac
    21121 cgcaacctcg tcgtcggcta tgtcctgaac aaacccagta cggaacaccg ccgacagatt
    21181 gacgatgccg tcgccaaatc cctgcaagcc atacccgaca tccttgccgg caaatgggaa
    21241 gaagcaaccc gcttcctgca cagcaaatga cccgatgccg tctgaagccc tttcagacgg
    21301 catgttcccg atttccatat ccgaacagtc atgaccgaac tcaagcagct tatccaaacc
    21361 gaatccatcc ccgtcatcga agaaaccctc gatttcctgc tgtacgaatg cagcatcgac
    21421 gacgcaccat ccgccgaaga agtggcacaa tggcgcgaca tacttgccgc acgcggcggc
    21481 aaattcctgc gcctgtccaa aatctgccaa acgtggctgg acgaggaggc ggcatgaagc
    21541 tgccgcgcaa ccgcttcagc ctgctttccg cattgtggtt tgccggcggc atctattcgc
    21601 tgctcttcaa agctgccgac accgcgccgc cgccgtttcc gcatttcgac aaagcagcac
    21661 accttgccct gtttttcgca caaatctggc ttttgaccaa agcattcaaa accggaaaac
    21721 ttcccatccc ctaccgcagc ctgatggtct ttgccctctg tttcgccctc ttcagcgaat
    21781 gcgcgcaggc atggtttacc gcaacgagaa ccggcagttt gggcgatgtt cttgccgata
    21841 tggcaggtac ggttctcgca ctctttgccg cccgcgccgc cgaccgcccg gactgaatcg
    21901 gtttatttcc cccaaacagg atgcacctct tcccgataca tcctattttt ccccttacat
    21961 atcaatccca tctcataaac aaaaccgaca aatcgtttac aatatattta cactacatca
    22021 gattacaaat atactcgaac cagttgtaga atcggcggtt ccacaaaccg tttcggattg
    22081 cacgacacaa aagaaaaacc gattttattg ccttaaagga gtattcatga acctgcatgc
    22141 aaaggacaaa acccagcatc ccgaaaacgt cgagctgctc agtgcgcaga agccgattac
    22201 cgactttaag ggcctgctga ccaccattat ttccgccgtc gtctgtttcg gcatttacca
    22261 catcctgcct tacagccccg atgccaataa aggtatcgcg ctgctgattt tcgttgccgc
    22321 actttggttt accgaagccg tccacattac cgtaaccgca ctgatggtgc cgattctcgc
    22381 cgtcgtactc ggtttccccg acatggacat caaaaaggcg atggctgatt tttccaaccc
    22441 gattatctac atttttttcg gaggcttcgc gcttgccacc gccctgcata tgcagcggct
    22501 ggaccgtaaa atcgccgtca gcctgttgcg cctgtcgcgc ggcaatatga aagtggcggt
    22561 tttgatgttg ttcctcgtta ccgcctttct gtccatgtgg atcagcaaca ccgccaccgc
    22621 cgcgatgatg ctgcctctag caatgggtat gctgagccac ctcgaccagg aaaaagaaca
    22681 caaaacctac gtcttcctcc tgctcggcat cgcctattgc gccagcatcg gcggcttggg
    22741 cacgctcgtc ggctcgccgc ccaacctgat tgccgccaaa gccctaaatc tggacttcgt
    22801 cggctggatg aagctcggcc tgccgatgat gctgttgatt ctgcccttga tgctgctctc
    22861 cctgtacgtc atcctcaaac ctaatttgaa cgaacgcgtg gaaatcaaag ccgaatccat
    22921 cccttggacg ctgcaccgcg tgatcgcgct gttgattttc cttgccacag ccgccgcgtg
    22981 gatattcagc tccaaaatca aaaccgcctt cggcatttcc aatcccgaca ccgttatcgc
    23041 cctgagtgcc gccgtcgccg tcgtcgtctt cggcgtggcg caatggaagg aagtcgcccg
    23101 caataccgac tggggcgtgt tgatgctctt cggcggcggc atcagcctga gcacgctgtt
    23161 gaaaacatcc ggcgcgtccg aagccttggg acagcaggtt gccgccacct tttccggcgc
    23221 gcccgcattt ttggtgatac tcatcgtcgc cgccttcatt atttttctga ccgagttcac
    23281 cagcaacacc gcctccgccg cattgcttgt accgattttc tccggcatcg ctatgcagat
    23341 ggggctgccc gaacaagtct tggtattcgt catcggcatc ggcgcatctt gtgccttcat
    23401 gctgccggtt gccacaccgc ctaacgcgat tgtgttcggc acgggcttaa tcaagcaacg
    23461 cgaaatgatg aatgtcggca tactgctgaa catcctctgc gtagtattgg ttgctctgtg
    23521 ggcttatgct gtactgatgt aaaccatcga cctaaacaac aagaccgtct gaaagaatat
    23581 ttttcagacg accttgaagt ttgtcgttca gacacaattt gtcgaatcat tcaaaaccgg
    23641 attctaacga aaggaaaccc atgattatcc tgcacaccaa caaaggcgac atcaaaatcg
    23701 aactcgattt cgacaaagcc cctgttaccg ccaaaaactt cgagcaatac gtcaaagacg
    23761 gcttctacga cggcgtaatc ttccaccgcg tcatcaaagg cttcatgatt caaggcggcg
    23821 gcatggatga aaacatgaac gaaaaagaaa cccgcgatcc gattcaaaac gaagcgtcca
    23881 acggcctgcc caacgataaa tacaccatcg ccatggcacg cacttccgac ccccattccg
    23941 ccagcgcgca attcttcatc aacactgccg acaacgcttt cctgaacttc cgttctaaag
    24001 agctgtacgg caaaaccgtc gtccaagact ggggctatgc cgtattcggt aaagtcgttg
    24061 acggttttga tgttgtcgat gccatcgaag gcgtatccac caaacgccac ggctaccatg
    24121 acgacgtacc gagcgaacct gtcgtcatca ttaaagccga agcggtataa accggcaatc
    24181 cgaaagcagc ctgcacaaag gctgcttttt ttgtttcagg cggcataaaa tccaagcaca
    24241 tatgccgtct gaacacctcc cgcaagcctt cggacaatgt tgcaacgtgt taaaatagcc
    24301 tcctttcctg aaaccacaac aaggaaacca tcatggcaaa tattgcccgc gacatcttca
    24361 aagcctacga catccgaggc attgtcggca aaaccctgac cgacgatgcc gcctatctca
    24421 tcggcagggc catcgccgcc aaagccgccg aaaaaggcat gacccgcatc gcgctcggac
    24481 gcgacggacg cttgagcggt cccgaactga tggagcacat ccaacgcggc tttaccgaca
    24541 gcggcatcga cgtcctcaac gtcggtatgg tcgccacgcc catgctctat tttgccgcca
    24601 tcaacgaatg cggcggcagc ggcgtgatga ttaccggcag ccacaatccg cccgattaca
    24661 atggtttcaa aatgatgctc ggcggcgaca cgctcgcagg cgaagccatt caagaacttt
    24721 tagccattat tgaaaaagac ggttttgttg ccgccgacaa acaaggcaac gtaaccgaaa
    24781 aagacatctc cggcgaatac cacaaccaca tcgtcggcca catcaaactc aaacgcccga
    24841 tgaacatcgc tatcgacgcg ggcaacggcg tgggcggcgc gtttgccggc aaactctata
    24901 aaggcttggg caacgaagta accgagcttt tctgcgacgt ggacggcaat ttccccaacc
    24961 accatcccga cccatccaaa cccaaaaacc tgcaagattt gattgccgcg ctgaaaaacg
    25021 gcgatgccga aatcggcttg gcgtttgacg gcgatgccga ccgcttgggc gtagttacca
    25081 aagacggcaa catcatctat ccagaccgcc aactgatgct tttcgcgcaa gacgtgttga
    25141 gccgcaatcc taaagccaaa gtaattttcg acgtcaaatc cacccgtctg cttgcgcctt
    25201 ggattaaaga acacggcggc gaacctgtga tggaaaaaac cggccacagc tttatcaaat
    25261 cctccatgaa aaaaaccggc gcgccggttg ccggcgaaat gagcggacac atcttcttca
    25321 aagaacgctg gttcggcttc gacgacggtc tgtacgccgg cgcacgcctc ttggaaatcc
    25381 tgtctgcctc cgataatccg tccgaagtgt taaacaacct gccgcaaagc atttccacgc
    25441 ccgaactcaa catcgccctg cccgaaggca gcaacggcca tcaggttatc gacgaactcg
    25501 ccgccaaagc cgaatttgaa ggcgcaaccg aaatcatcac catcgacggt ctgcgcgttg
    25561 aatttcccga cggcttcggt ctgatgcgtg cttccaatac cacgccgatt ctggtgttgc
    25621 gttttgaggc ggatacgcaa gaagccatcg agcggattca aaaccaattc aaagccgtca
    25681 tcgaaagcaa tccgaatcta atctggcctc tgtaaacaca ggaaaaatgc ttaatcaaat
    25741 cttggtagat tggatttggc ttaaaaatct ccgcgtccgt tcgtgtttgg ggtggtcgaa
    25801 caactctttc gggctgccct gctctacgat aacgccgccg tccatcacga caacggtcgt
    25861 ggcaacttcc agcgcgaact tgatttcgtg ggtaacgacg accatcgtcc aaccttcccg
    25921 cgccaattcc ttcatggcgt tcaacacgtc ttgcaccaat tcagggtcga gtgcggaagt
    25981 gggttcgtca aacaacatca gctcgggctg aatcgccaat gcgcgggcaa tgccgacgcg
    26041 ctgctgctga ccgccggaaa gctggtaggg atacaaatcc actttgtcgc ccaagccgac
    26101 tttttccagc agtttcagag cctcttcgcg cgcttgggcg gcaggcttgc cctgtacggc
    26161 aaccggtcct tccattacgt tttccaaggc ggttttgtgc ggaaagaggt tgtattgttg
    26221 aaacaccatg cctgatttgc ggcgcagtgc caaaatatcg tgtttgcttg gttttttaga
    26281 aaaatcgatt ttcagcggtc gctcgttgtc gaactcgatt tgtccgtctt cgggcatttc
    26341 caacgcgttt aggcatcgca gaaacgtcgt tttgcctgag ccggaaggcc cgaggataac
    26401 gaccacctgc cctttgccca catccaaatc gatgccgcgc aaaatagtgt tttcgccgaa
    26461 ggtcttatgg atattgcgga ttttaatcat aacaactcct tatttggcga cataacggtc
    26521 gaaacgtttt tccaaacgtg cctgaatcag gaacagaacc ttgcagaaac accagtaaac
    26581 caatgcggct tcaatataga cgggcaaaaa gtcgtaagtg cggtttgccg tttcctgcgc
    26641 gacgcggaac aattcagtta ccgttaccac cgctgcgagc gaggtgtttt tgaacaagcc
    26701 gataaactcg ttgctcaaag gcggcacggc aacgcggaat gcctgcggcg cgacgatgcg
    26761 gcggaacgtc tgcatatagg tcataccgat ggagaaacct gcttcccatt ggcctttagg
    26821 tacggacaaa attgccgcgc gtatggtttc ggaagcgtat gcaccgacat tgagcgagaa
    26881 gccgataatg gcggcgggaa tcggatcgat atagataccg acggacggca gcccgtaaaa
    26941 cacaatcaca agctgcacca acagcggcgt accgcgaatg acggaaatat aaaattccac
    27001 caatttcagc aggattttcc gcgcaatgcc gccggcgggc ataatccgca ccaaagccac
    27061 ggctacggca ataaccatac cgataacgaa agaagctgcc gccaaaggca gagacaccgc
    27121 gaagccggct ttgaccatag gcaaaaacgc gctgacaatc atatcggcgc gtgtttccgt
    27181 cataaacggc agcgaagcaa ggaaattatt gaacactgat gtcttttccg aagaattgtt
    27241 cgcccagttt tttcagcgta ccgtcggctt tcagctcgtt gattgccgta ctgaatttcg
    27301 ccacggcttc gtcattgccc ttgttgacaa tcaggccgga accgactttt tcatcggcag
    27361 gtgcggacca aacgattttc acgcccgcat tcgggttttt cttcagatag tccaaaaccg
    27421 ccaattcgtc gttcagggtt gcatcggcac gtttttgttc aatcagggtc agcgattgcg
    27481 ccaaaccgtc cactgccacc aaatctgcgc ctgcagcttt ggctttttcg ccgtagttgc
    27541 tggtcaggga ttgtgcggtt ttcacgcctt tgatgtcgtc gatagatttg atgttgctgt
    27601 cgttacgggc aaccaatacg gcaccgctcc agctgtaagg atcggatttg tcgaatgtcg
    27661 cttggcgttc ggggctggtc agaccgactt ggtttgccac cacgtcgaaa cgccccgcct
    27721 tcaaacccgc catcatcgaa tcccattgcg tttctttaaa ctcgactttc acgcccagtt
    27781 tttccgccac ggcgcgggtt acttccacat cgtaaccggt cagtttgccg tccttgtcgt
    27841 ggtaggtaaa cggtgcgtaa gtgccttccg tgccgacggt aaccgtgcct ttattgttga
    27901 tgcgctcgat taaagaaccg gaaactgccg attgtgcagg cgcggaagat gctccgctgc
    27961 cgccttccga accgccgcag gccgccaaaa ccaatgcggc aataccgccg agtacgaatt
    28021 ttttcaacat catcctctcc tgtaaaaagt gtgcaagtgc ggcattctaa gtgaaattgc
    28081 ggcaaacaca aagaatcctt tttttatatg gttattccaa atcggaatgg aaaagatatg
    28141 ccgaagccgc cggaagcgga aataaagggg gcgggaaagg cggtgggcgg cacgaaaccg
    28201 aaaagcggca tcaggtatgc aaacggcgga ttcacgcacg ccgttccggc aaaaatccgc
    28261 ctgccgattc ggtgctataa tccatccgaa acagatgaca ccaaaaagga acatctcatg
    28321 aacacgcccc aatccgccat cattcccgac cacgcccaag ccggcatctt tatcgaagcc
    28381 gacttcgccg ccaaccgcct caacgatatt aaagccgcct gccgcgcttc gctcgacgcg
    28441 ttaactgcct tgaaagcccg ctttccggac gatattttgg gtctgaccat cgccttcggc
    28501 agcaaagcct gggcaacatt cggacatacg gacgaaggca gtgaaatcaa acccttctcc
    28561 gaaatgggca acgggcttgc gccgtccacg cagcacgata tgtccatcca catccaatcc
    28621 ttccgccaaa acgccgccta cgcgcttgcc caatccgttt taggcgcgtt cggcgacagc
    28681 atatgcgtcg cgtccgaaga acacggtttg cgcctgtatc aggatcgcgg actggacggt
    28741 ttcgtcgacg gcacggaaaa cccgcagggc gatgaaaatg tccgcgaagt cgccatcatc
    28801 cccgaaggaa aacccgatgc gggcggcagc tatgtcctgc tgcaaaaata cctgcacgat
    28861 ttgaaaaaat gggatgccgt ccccgtcgcc gaacaggagg cctcggtcgg acgcggcaag
    28921 gaaaccgacg acgaattcgg ccgcgatgtc cgcctgcccg attcgcacct cggccgcgtc
    28981 aacctgaaag aaaacggagt cggcctgaaa atcgtccgcc gcagcctgcc cttcggcaaa
    29041 atcagcggcg aacacggttt gatgtttacc gcatactgcc gcacgctgca caatatcgaa
    29101 gcgcaattat tgagtatgtt cggcgacacg gatggcaaaa ccgacctgct gctccggcac
    29161 ctctccgccg ccgtttcggg cggatactac tacgcgccgt ctgtcgaacg cctgcaaaac
    29221 ctctaagcca gacgatgccg tctgaaaccc tttttcagac ggcattttcc cgttttaccg
    29281 tccaaccgtt ttccgagcgc gcactatgtc tgcacccatc caagcatttg acccgctgac
    29341 cgtccccatt gccggcacca acctgattga ggcctctgcc ggcaccggca aaacctacgg
    29401 catcgccgcc ctgtttacgc gcctgatcgt attggaacaa aaaaacgtcg agcgcgtatt
    29461 ggtcgttacc ttcaccaaag ccgccaccgc cgagttgaaa acacgcctgc gcgcacgttt
    29521 ggacgatgtg ttgcaggttt tagaaagtaa agaaattgcc aaacttggag acgacacgct
    29581 ttcagacggc atcgccgcct actgcgccga acaccacaaa ggcgacacct tcctgcccgc
    29641 actcttaaaa caggctttgc aaaaagaggg ccggacgcgt ctgattgtcc gcctcaaggc
    29701 cgccatcggg caattcgaca acgccgccat ctataccatc cacggcttct gccaacgtat
    29761 cctgcgcgac tacgccttct tgtgccaagc accgttcgat gtcgaactga ccgaagaaga
    29821 cggcgaccgc ctgcttatcc cggcgcaaga tttttggcgg gaacgcgtca gcaatgatcc
    29881 ggtgcttgcc gcattggcgt ttaaacgcaa agctgtgccg caaaccgtcc ttgcccaaat
    29941 ttcgcgctat ctttcccgcc cgtacctgaa tttccgccgt ccgcaggcgg atttgaaaca
    30001 ggcgcagcgc aacgccgaaa cctcatggca aaccatctgc cgcctgctgc ccgagctgga
    30061 agccggcttt tggcgcatcc atcccgacct caacggcaac agttaccgca aaaacagctt
    30121 cggcaacctt tttaaagaac tggcacaaaa atccgccgcc ggacaactgc cctttctgga
    30181 caaagacacg cacgacagac tcttgaaact tgcgtccgac aaactcgaag ccgcccttaa
    30241 aaaaggcaaa acgcccgatg cggcagcatt tgccgaattg cagaaactgg cagacttcgg
    30301 gcgcgatttg aacgcactcg aagaagcgga agaagcaaca atgatccggc tgcaactgga
    30361 tttaatcgga tacctcaacc gcagccttgc cgagatgaaa aaatcgcgcc gcgaacgcgg
    30421 tttcgacgac ctgctgctcg atgtccacac cgcgctgacc gacaatccgc acgccgaaac
    30481 cctcgcccgc gccgttgccg aaaactggga aaccgcgctg atcgacgagt tccaagacac
    30541 cgacccgctg caatatgaaa tcttccaaaa gattttcatt gcccaaaacc gcccactgtt
    30601 tctcgtcggc gatcccaaac aggcgattta cagctttcgc ggagcggaca tttacgccta
    30661 cctgcaagcc gccggagacg cgcggcaccg ctacacgctc gccaccaact accgcagcca
    30721 cgccgcgctt atcggcagca taggcgcgct gttccgcctc aaagaacgcc cgttcgtttt
    30781 ggaaaacatc ggctattcgg aagtcggtgc ggcgcgtgcc gaaagcaggt tgtcccccga
    30841 acgtcctgcc gtacaggttc gttggctgca cgaaaacgac aatgaaaaag ccaacaaaga
    30901 cgttttgcgc cgccgtgccg ccgactattg cgccgacgaa atcgcccacg cgctcaacga
    30961 agccgccgga ggccgtctga atttcaaagg ctgcccgttg cagtcgggcg atattgccgt
    31021 gctggtacgc acgcacaacg aggcggtgat ggtttccgcc gccctgaaaa aacggcaggt
    31081 gcaaagcgtc ctgctttcgc gcgaatctgt gttcgcctcg cccgaagccg ccgccctgtc
    31141 cgcactcatc ggcttctggc tcgaaccgcg ccgcgccgga acgctgcgct ttgtcctgac
    31201 aagcggcata ttcggttatg acgcgcagca attgcacgac ttcaaccaaa acgaaagcga
    31261 gattttgcat tgggcggaat ctgcccgaac cgcactcgac aactggaatc aatacggcat
    31321 tttcgccgct atgcagcaat tttcccaaac acacggcatc gaaacgcgcc tcttaagccg
    31381 gaacaacggg cgcagcctga ccaactattt ccaactgctc gaactgcttg ccgccgaaga
    31441 cgcgcaaaac cgcaacccag ccgcgctgca caaatggttg cgcgaccaaa tcagcctggc
    31501 cggcaacaac agcggcgaca accgcgccat ccgtctggaa agcgacgaag atttggtcaa
    31561 aatcgttacc atgcacgcct cgaaaggttt gcagtatccg ctggtgtact gcccgtttgc
    31621 gtgggacgcg caagataccg ggccgtccga ctggcaaatc ctccaccaaa gcgcaaaccg
    31681 aaccgaactg ttggcaaagg cgcaactgtc ggaagacgaa cagaaacaat acgccgatga
    31741 agaaatggcg gaacgcctgc gcctgcttta tgtcgcactg acgcgtgccg aggaacagct
    31801 caacatctac gccgcgtact ccaccaatac tgccgacaac cccctcgcct acctgattga
    31861 aggctcgccc caagacagcc gtgaaaccgt ccgccgtgcc tatgcgtgtg aaaaagacgg
    31921 catcgcgatg ctcaaacgca actggcggcg cgtggcggac aacgcccctt ccggcacaaa
    31981 tttcgccttc accgaaaatg cgccgccccc tgccgcctat cgcagcaacg ccggtcaaac
    32041 cgccgaattt gccgcaaaca gcattcccga acgcggattc cgatttgtcc gccacactag
    32101 ctttaccgcc ttaagccgcc atacccaaac gcccgacggc ggcgaagaag atgcctgccc
    32161 gtccttggat gccgccgaaa cctcggtgcc ggcgatgccg tctgaaacgc cgacggcttc
    32221 agacggcata tcgatacacg attttccgaa aggcacgcag gcggggctgt gcctgcacga
    32281 aattcttgaa gatttcaaat tcggacaagc agccgccgga caggaaaccc tcattgccga
    32341 caagctgaaa aaatacggtt ttgaagaaat atggctgccc gccgttgccg aaatggcgga
    32401 agcctgccgc aaaacgccgc tgacgggggc atacgacctg tccgacatct cacccgagtg
    32461 ccgctgtccc gaaatgggct ttaccctcca caccgaagac ttcagcctca aacgcctgcg
    32521 cgactggttt gcccgcgacg acatcaggct gcccgaagtc tgccgtgccg ctgccgaaac
    32581 gctcgacttc cacaccgtca acggcttttt aaacggcttt gtcgatatgg tctgccaaga
    32641 ccccgacggc aatatctgcg tcatcgacta caaatcaaac cacctcggta cggacgcatc
    32701 cgcctacacg caacaggcaa tggacgaagc cgtcgcacac caccactatt accttcaggc
    32761 actgatttac gccgttgccg ccgcacgcta cttcaagctg cgcggacaac cgcccgccgc
    32821 cgtttccgtc cgctacctgt ttttgcgcgg attggatggc aaaggcggcg gcgtttggcg
    32881 ttgggacata gatgccgccg ctttggaaca gataaaataa cttcccgtcc tttcggacaa
    32941 caaccgcagg aaaaccctat gaatatcaaa cacctgatta ccgccgcact cattgcctca
    33001 gccgcctttg ccgcgcaggc agccccgcaa aaacccgtat ccgccgccca aaccgcgcaa
    33061 catccggccg tttggatcga tgtccgttcc gaacaggaat ttagcgaagg gcatttgcac
    33121 aacgcggtca acatccccgt cgaccaaatc gtccgccgca tacacgaagc cgcgcccgac
    33181 aaagacacgc cggtcaacct ctactgccgc agcggacggc gtgccgaagc cgcccttcaa
    33241 gagctgaaaa aagcaggtta tacaaatgtt gccaatcacg gcggttatga agacctgctc
    33301 aaaaaaggga tgaaatgacg caatgccgtc tgaaaggaca ggctttcaga cggcatacgg
    33361 cgttccggca cgcggcgcaa catcgggcgc ggcgcgcgcc gccccgtccg aagcctgata
    33421 aacaaaccga taaggaacaa tgatgtttca cgacacataa agcaccgccc tatgtgttgc
    33481 cctgatttgg aaagggttac acccctcccg aataaagtct gattctaccg tttcaaccaa
    33541 aaaggaaaaa ggaaatacga tgaaacacat actccccctg attgccgcat ccgcactctg
    33601 catttcaacc gcttcggcac accctgccgg caaaccgccc acccaaaacg aaaccgctat
    33661 gaccacgcat accctcacct cgaaatacag ctttgacgaa accgtcagcc gccttgaaac
    33721 cgccataaaa agcaaaggga tgaacatttt tgccgtcatc gaccatcagg aagccgcccg
    33781 ccgaaacggc ttaacgatgc agccggccaa agtcatcgtc ttcggcacgc ccaaagccgg
    33841 cacgccgctg atggtcaaag accccgcctt cgccctgcaa ctgcccctgc gcgtcctcgt
    33901 taccgaaaca gacggcaaag tacgcgccgc ctataccgat acgcgcgccc tcatcgccgg
    33961 cagccgcatc ggttttgacg aagtggcaaa cactttggca aacgccgaaa aactgataca
    34021 aaaaaccgta ggcgaataag cccgtcgccg acatatggcg gatggaaatc aaatgccgtc
    34081 tgaaacccga tttcagacgg catttccttt caatccctat cttcccgaca aatatcaatg
    34141 gcttcctgca aactcgaaac gccgtagatt ttcaggttcg gaaactcttt ggcgttgcgc
    34201 ggcatattgg ctttggggac gatggcgcgt ttgaagccga gtttttccgc ttctttgagc
    34261 cgctcttgtc cgcgcgcgac ggggcggact tcaccgctca aaccgatttc tccaaatgca
    34321 accatttttt cgggcagcgg gcggttgcgg aagctggaaa gcatcgcgag gatgactgcc
    34381 aaatccgccg ccggttcgcc gattttcacg ccaccgacgg cgttgaggaa cacatcctga
    34441 tcgaaacagg cgatgccgcc gtgtcggttt aacacggcga gcagcatcgc gaggcggttt
    34501 tgttccagtc cgacggtaag gcgtttgggg gtgaatccgt gcgcgtcatc gaccaatgcc
    34561 tgaatttcaa ccaaaagcgg tcggctgcct tcctgcgtaa ccaaaacgca cgaaccgggc
    34621 gtgtcgtcgc ggtagctggc aaggaagatg gcggacgggt tggacacacc tttcaaaccg
    34681 ttttccgtca tcgcgaacac gcccagttcg tttgccgcgc cgaagcggtt tttgatggcg
    34741 cgtatcatgc ggtagttgga atgttggtcg ccctcgaaat acagcacggt atcaaccatg
    34801 tgttccagca cgcgcgggcc ggcaatcgcg ccgtctttgg tcacgtgtcc gaccagtatc
    34861 atggcgatgc ccatctgttt cgccatgcgc gtcagttggg cggcacactc gcgcacctgc
    34921 gacacagaac cgggggcgga agtgatttgg tcggaataca tggtttggat ggaatcgata
    34981 accacgactt cgggctgatg ctgtttcaac gctgcttgaa tcgcttccat gcggatttcg
    35041 gcaagcaggt tcacgccttc ggtgggcagt tccaaacgct gcgcgcgcag ggcgacctgt
    35101 tgggcggatt cttcgccgga aacgtatagc actttacggc tttgcgccat tttggcgatg
    35161 gtttgcagca gcagcgtaga tttgccgatg ccggggtcgc cgccgagcag gatgaccgca
    35221 ccatcgacca aaccgccgcc caatacgcgg tcgagttcgc ccatgccggt cggattgcgc
    35281 ggcacttcgg tggcggtcac ggaggagagg gattggacgg tcgaggtatc cgccgcccaa
    35341 gattggaagc gggcgttttt cggctcgggc gcggccaagc tttcctgaag tgtgttccac
    35401 tcgccgcaat gcgggcattt gccctgccat ttcggggaag tgccgccgca ttcggtgcat
    35461 tggtaaaggg ttttaagcgt ttttgccatc ggttttccct acatctgaaa acaaaaatgc
    35521 cgtctgaaaa tagggacggt tgggattgcg ccaatacggc aacaccgccg ccgtcatgta
    35581 ctatccgtac acggcggata ttgccgcatt gccttatttt taccctaacc ggccatctct
    35641 cggcttcaaa cggcatatcg ggcagtttcc tgccggctta cccgccgtga tactgccgcg
    35701 acagctcgtg tacggtgtcg actaagattt tggcgtgttc ggggtcggcg tgttggttga
    35761 tgccgtgtcc gaggttgaag acatggccgc tgccttgtcc gtagtcggct aggatgcgtg
    35821 cgacctcggt gcggatggat tccggcgtac cgaagagggc gaacgggtcg aagttgcctt
    35881 gcagggcgac ttgcttgccg acgcggcggc gtgcttcgcc tatgttgcac gtccagtcca
    35941 agcccaatgc gtctgcgcca atttctgcca tgctttccag ccacagcccg ccgcctttgg
    36001 caaatacgat aacaggcacg cgtctgcctt cgctttcgcg tttgagtccg gcgacgatct
    36061 ggcggatgta tttgaggctg aattctttaa acgcctcatc gctcaagacg ccgccccaag
    36121 tgtcgaaaat ctgcacagcc tgcgcgcccg cgtcgatttg ggcgttgaga taggcggtaa
    36181 cggcttgagc attggtatcg aggattttgt gcagcaaatc ggggcgcgag tacatcatgg
    36241 ttttgatggt gcggaattct ttgctgctgc caccttcaac catgtagcag gcaagtgtga
    36301 acgggctgcc ggagaagccg ataagcggta cgcggccgtc cagtgcttta cggatggagc
    36361 tgacggcatc aaagacgtat tgcagttttt ccatatcggg gacgtgcaat ttggcaatgt
    36421 cgtcctcgtg ttgcagggcg cgtttgaatt tcgggccttc gccttcggca aaatacagtc
    36481 ccaagcccat tgcgtcaggg acggtcagga tgtcggaaaa caaaatcgcc gcgtccaaat
    36541 cgaaacgttc caaaggttgg atggtaactt cggtcgccaa ttcggtgttt ttgcacaaat
    36601 cgaggaagct gcccgctttc gcgcgtgtgg ctttgtattc gggcagataa cgccccgcct
    36661 gacgcatcat ccaaatcggc gtgtattcga cgggctgttt aagcagggcg cggaggaagg
    36721 tatcgttttt taaaagagtc atacgggttt tctgtcagtc tgtttgtcaa gtgaaatggt
    36781 tttcagacgg catttcaacg atgccgtctg aaggtttcaa caggtttcgg cggaaggtcg
    36841 gttttcctcg gcaacgcttg ccaaaaccaa gttgcgctgc gcctccgcct tctgcggttc
    36901 tccggtttcg tcaaaaacct ttgccaaaac caaacgcgcg gaaatactcg gctttaatgc
    36961 aatgctcgct tcaaggtagc cttttgcctt gccccaaagt ttgcggccgt aggcgagccg
    37021 accgagatac atcagcagaa gcgcgttatc gggctgttct ttcagccaag catcggcaaa
    37081 atcgatggct ttctgctgat cgcgttcgcc caaaaagcgc acgctttcga caaaggcttc
    37141 caaaagctcg gggcggcggt tgtgcggata atgctgtttg acccatttga ccgcatcggc
    37201 atacagtccc aaacgttcgt acttttccgc aaccgatacg ctcaattccc cgtttttgag
    37261 gctgtcggga atccgcttca ggcaggtttt caaagcggcg gcatcggcag catccgccag
    37321 ctggcggcgg tatgcccaat tttgataccg ttccatttcc gatttgccca acgcgcccgc
    37381 cttggaaagt ttttcggttt ttgccagaac ctgcaacgcg tcgcccctgt cgaaagcgta
    37441 acgaagttgc agacgcacga ggcgcgtaag gttggcattc atcttcgccg ccgcatgaag
    37501 attggcttcc gccgcttcgt aatcgcgccg gttcaacgcc gattccgcca acaaaagata
    37561 acgggaaagc tgctgctttt ccggcagttt ggcgatttcc gcaagataac ggtcgcgcag
    37621 ctcgatgttt tccatctgcc cggcggcatg tgcgcccaac atcaatgcca aagtccggtt
    37681 gtctccggcc tctttgttga ccaacacgcg tgaggcttct agttccgcct tttcaaaacg
    37741 cccttcaaaa tacgccaaac ccgccttgtt caaggcaagc gcggccttgc ggcctttacg
    37801 cgccgaaccg aaacgctgca tcttttcggg gatattgaat acgccgataa tgaatttgaa
    37861 caggaaatac cacaccacga cggcaatcag cgaacctaac acaaaggcgt gcaggttgat
    37921 tctgagcatg gtctgtccga gtacgatata cacgtcgccg gtgtaaatgc ccgaagccag
    37981 tgccagtccg acggcggcgg caaacaggac gacaatccag actaccgttt tcatgcgcgt
    38041 tctcctttag cggacggcga acgccctgcc ttttccggta cgggctgttt ttttgcaggc
    38101 tgttccggtt tgcgttccga aggcagggac ggtgcttcta tggtcttcac atccgatgcg
    38161 gcagccggtt cggaagctgt gttttcgttt gccggttcgg aagtcgcctg ttccgcttct
    38221 tgattttccg ccgtcgtcat gcgcgtaccg tcgcgatagg cgcggacggc atttaggctg
    38281 tttttcaaac cgtcatccgc cgtcatgcgc acatccaacg ccttcaattc cgccaattcc
    38341 ttcaaccacg actgcgtggc gggggatttg gcatcaaaat actgtctgac ggcggtttcg
    38401 gcattgttca aatcgccctg atagacttcg ctgttgcgct gcattaatgc agtccgcgca
    38461 tccaaaaggc ggagacgcag gttttcacgc acaaaatagg cctgttcggg agaaatcagc
    38521 atggcatcgt tgttttccaa acggcggatt tcgaccagcc ccttcaatgt gccgagggat
    38581 ttttcccata cgttctgcca ccatgaagcg gaaacggctt cgttgcgtgc ctgtacgctc
    38641 ggtttcaata cgccgtccaa aatcagcggc agtccggata cggcgatttc cagcctgtcg
    38701 aggcgcaatg ccgtgccgga aatatcgaca tagggacggt ttttcagttc cgccaagtcg
    38761 ctgctgaccg cctgcttgat cggcagaagc tctgcctgat tgaaacggga caggcggctg
    38821 tcgatatgct ccaatacgcc gactgccgtt tggatattgc cagtcaacac cagctgttgc
    38881 gccgccagat tgagtatggt ctcggtttcg tccaccagcc aatcggcgcg tcctttggtc
    38941 aactcgcgat aggatttttg catttccaag atttgttcgc cgtttgcttt gacgtttccg
    39001 tccaaacggt cgagctctga ttgtatggcg gcttgccggt tgaggttgtc tttgagcagg
    39061 gcggcgtttt ccgactcgcc caaagcggct ttgtcgattt tttggttgaa tgccagctct
    39121 tggtttttca agacattctg tccttggaca aacaaaaaac cacttgtacc caaacccaat
    39181 gccgccaata ccagggcgca gactgccaaa gcgttgctgc cggactgttt gacgataaaa
    39241 ggagcggcag gaatgtgtac ttctgtttcg ttttctttgc gtggggaaga ggtttcagac
    39301 ggcatttctt ttgatgcctg tatctcgcgt acgggttcgg atgatttgtt ttcaggttcg
    39361 cccaccgttt tgctccttat taatttgagg ttccgggaaa gaccatgccg tctgaaacgg
    39421 aaatggaaga atgggaaagc gcggcttcca gcgtagggac ggtttcgacc gaacacacgc
    39481 cttcgcgctt caatgcctcc gcaatgcgcg gatgatgggt aaagtatagc aaggatttga
    39541 agaatcggga aaattgcggc ggaagctgcc cgaacagcaa gcgcaccagc tcggtcgacg
    39601 taatataggc ggcggcaata ttttcggttt ggaaattttg aaagttcaaa ggtttatgcc
    39661 gtctgaaata gacttctgcc atctccgtcc gaaaaccttt ctcctgcaag gcattcatca
    39721 gaaaatcccg cccgccgtgt ccgcgcacaa acaatacgcg tgcaccttcg ggcagactgt
    39781 tccaaaccgg caggcgcaaa accgcctcgc tgtcgtttcc gtcatgaggc gcaatgaccg
    39841 ttctgcccaa atagcgttcc aatgcgcggc ggctgccctg ccctacggca atgtgtatct
    39901 ttatgccgtc tgaaaggtta aggtacggga cggcggtttc aacggcggtc ggactgaccc
    39961 aaaacacggc atccgcacgg gcatattgtt cggacaaaag ttccagtccg gcagcatctg
    40021 cttcgatttc aatcgggctc aacacttccg cctgccatcc ggcacgacgg caggtttcca
    40081 catcgtcctt cgccctgccc gacggtcgga caatcagcat gatgggtttc gctgtttcca
    40141 tcgccttatc cgccccgatt accgggaaaa atcagcatcg ttgaaattcg cctgcgcggt
    40201 ttgcggcgta cagccgaccc gtgcgacttc cttgccccta cgctccacaa cgacaccgct
    40261 gctttccgta tgagtcacgg aatcgaagcc tgtccatatg gtgtacataa attcgccgtt
    40321 ttggaatttc atcgttgccc aacgaccgct gcccatacct tgccacctgt cggaacgccc
    40381 cgacagatca gctttgctgt tgcgtatggc aatttctttt tttgccgcac tgccgaacga
    40441 atattcgtaa agattgcggt tgattttttg aacttctata tatttgttgt tgtccgtttt
    40501 acaggaaaac accgtatccg cccacaccgt ttgggcggca gccgacagaa gcaatacggc
    40561 agaaaggaac atttttttca tcgtatttcc ttaatggttg aaaccccgcc actcggacat
    40621 ccgtccttcg tggcagcagc atcagatttt atttgagagg ggtgtaaccc cttccaaatt
    40681 agggcaacac atagggcaac gctttatgtg tcgtcctgtg tgttgaaaca tcattgcccg
    40741 tatcctgaaa tagaaaagaa aatgcccgtt ttacggtctt ctttccgaca aaatgccgtc
    40801 tgaaacggat ttcagacggc atggtcgaaa attattgcgc ggcaagcggc ttgagcagca
    40861 aggtttcgcc gctttgcaca actttttcaa gcaggtcgat attgggatgc ttgccgggat
    40921 gggcgcgcgc gtcggcaacg tgttcggcaa accatatcaa accctgttct gccgcttctt
    40981 tgtccaattt tccgtcaaaa cgttctgcca aagcgttgta caacttgagc gaaccgagct
    41041 taccctgaac ggcaggaatg tggtgaaccg ttttgccatc tgcatcctga acgtccaaac
    41101 cgcccagatg gtcgattgaa ggaaatgtgg cgagatagtc ttgaaaattc atatcaactc
    41161 cgaaaatcag tcagttagcg cgtgccgaaa atcttgtcgc ccgcatcgcc caagccgggg
    41221 atgatgtagc cgttctcgtt caagtggctg tcgagcgcgg cggtgtaaat cgtaacgtcg
    41281 ggatgcgcgt cgttgaccgc cttcacaccc tcgggcgcgg caaccagcac cagtgccttg
    41341 atgtttttgc agcccttggc tttcaaaagg tcgatggtgg caaccatcga accgcccgtc
    41401 gccagcatcg ggtcgataat cagggcagga cgttcgtcca tgctgtccac gaatttctcg
    41461 aagtaggaaa cgggtttcag cgtttcctcg tcgcgctgca gtccgactac gctgattttg
    41521 gcagtcggaa tcaggtcgag tacgccgtca agcataccca aaccggcacg caggatggga
    41581 acgacggtca atgttttgcc cttgatgcgg tcgccttcaa tctgaccgca ccatccgtcg
    41641 ataaggtatt tttcgatttc aaaatcacga cttgcttcgt acgccatcag gcgcgccagc
    41701 tctgtggtaa gcgttcggaa tttgtaggtg ctgcaatctg cctccctcat cagggttaat
    41761 ttgtggcgga cgagcggatg gttgataacg ttaacgttca tcgcggtgtt ccagtgtgat
    41821 tttgagacgc gccattatac ttttttttga tttgccggaa aagaaaatgc cgtctgaaac
    41881 acgttcagac ggcattgccg aatccatcag gctttcagca gctcttgcag ctcgcctgcc
    41941 tcatacattt ccatcaggat gtccgaaccg ccgacaaact cgccgttcac ataaagttgg
    42001 gggatggtcg gccagtcgct gtattcttta ataccttggc gtacttcggg attttccaat
    42061 acgttgacgg taacgtaatc ggtgcagcct gccgcgttca ggatttgcac ggcgcgggaa
    42121 gagaaaccgc attgcggaaa ctgcttcgta cctttcataa acaatacgac gcggtgtgtc
    42181 gttactactt ctttaatttg gtcgtggatg gaagccatcg cttattcctt ttgataaagt
    42241 tgacaaaatt tgtcaggtag gacgcattat acgcaaaagc ggaaacttcc cacaatattt
    42301 gccgtatatc gcgcagtatc aatgttgtga acggaaatat tcccactcct cgacaatcct
    42361 gctgtccggc aaatggcaca gggcatatcc gccgccgctt tcgtcttttt ttacttcccg
    42421 tctgccgcct ttggcaatgc agaatttgga agcggaattt gccatgcctg tccgaaacgt
    42481 gttggcaggc agccggcagc agcgcggaca tgcatacgga tgaattgcct tcatggtttt
    42541 ccttatcgtc ggtacagggt cagacgaccc gcatatcggg tactttttta aacagccggt
    42601 aaaagttttc cgtcgtatat gccgcaacct gttccaatgt ttggttccgc aatttggcga
    42661 tatgttcggc ggtatagcgc acaaaagccg gttcgttctg cctgccgcgt ttgggaacgg
    42721 gggcgaggaa cggcgcatcg gtttccacca aaatgcggtc gtccggcaca tatttcgccg
    42781 cctcctgaac caagggggca tttttaaagg taacgattcc cgagaaagaa atataaagcc
    42841 ccaaatccat ggcagcccgg gcaaaggctg tgtcttcgga aaaacagtgg ataacgcccg
    42901 aattaacccg gcattctttc aggatagaca aggtgtccgc cgccgcatca cgcgtatgga
    42961 cgataacggg cagtccggtt tgattggctg cttcgatgtg gtctgcaaag cgtttgtgtt
    43021 gccaggacaa atcgcctttg caccagtaat aatccaagcc cgtctcgcca atgccgacca
    43081 ctttcggatg ggcggctgca gcaaccattt ccgcaataga aaattcttcg gcttccttgc
    43141 tgtcgggatg tacgcctatg gtgcaataga tgtgttcgtg cgcttcggcg atggcaaaga
    43201 cttcggagaa gctttcccta ctgacgctga tggcgagcac ctgccccacg ccgtttgctt
    43261 ccatgttgga caaaacttcg ggcaggcgtt ctttcaaacc ttcaaaattg aggtggcagt
    43321 gcgaatcgat gatatgcatg gcgttacatg gtaaacgtag gtctgctgcc gccgattgtg
    43381 ttgcccagtt cgacttcgat ctggtctttg actttcaggc agttttcgtg ttttgtgaat
    43441 gccagcccca cccctttggg tttggaggaa gtacgcgcag gattgatcca ggcgactttg
    43501 gtcggcagga acagtttggg gaagttgagg atttctacgg caagcagaat atcgtccccg
    43561 atggaaaata cgtcgttggt ctgcacaaac agaccgccgt gttccaaaaa cggcatatag
    43621 gagctgtaca gcagattcat gtctttcagc tgcaacgaca tcatttttgc cggaatattt
    43681 tgtccgtctg acataattca cccgttctct ttcttcaatt ccaaataatt gatgagcaga
    43741 tgctcgatct gcattttgac atttaaagta tgaaacccgt aaggggcaag ctgtttgagc
    43801 atatcctccg ccgcaaatac attgcgcggg cggaaaccgg atgccgtctg aagcagcctg
    43861 tcttcataag cgggataata gacgggtttc atgtgttgca ggcacaatcc caaatcgacc
    43921 agccatttct gcatccaccc gacaaatacg tcgagcggaa gtttctcctt atcgaaaagc
    43981 gcggcgtaat ccaaaatctt caacaacctt ggttctgcca aaatatccaa cagtttaatc
    44041 cgcaacgcac ggacaccgtc cgcctcatca aacagcggcg cacccgaatg gaacgccaaa
    44101 aattcttccg gctccgccac acccctgtcg cgcagatatg ccaatgcttc cccatgcgaa
    44161 ggagcgggca aaaccatctt ccggcagcga cttttaatgg tcggtaaaac cttgtccgcc
    44221 gcgtggctga ccagcaaaaa gaccacttgt ggcggcggtt cttccaacac tttcaacaaa
    44281 ctgttggcgg cttggacatt catactttcc gcaggatgaa tcagaatcac gcgcaaaccg
    44341 ccccgtaccg aagtcaggta cacattatcg atgatttccc tgacggcatc gattttgatc
    44401 tgcaacagtt tgcgtccgtt ttcgggttcg tccgacaagg gggtgatttc gtaaaaatcg
    44461 ggatggcttc cctgtccaaa cagatggcag gacatacatt cgccacaggg tttgcagccc
    44521 ggtgcagggg tttcgcacaa cagtgccttc gccgcaaagc gggcaaatgt agttttcccc
    44581 gtatcttttt tgccggcaaa cagccatgca ttgggacgac gctcccaatg ttccgcaatc
    44641 tgccgccatt gctcattatg ccacggataa atcatttcaa accatccgtc cggaaatcca
    44701 taccgccatc agagcagttc caaatcgggg ctggaacttt gcgcgcggcg gctgcgcaac
    44761 tgtaccgcca aacgcaaatc gtgtgcggaa tcggcatttt tcaatgcttc ttgcagggaa
    44821 atatcgcctt tttcatacaa ttggtaaagg tgttgatcga aggtctgcat acccagggta
    44881 gtggattttt tcatcacttc tttgatttca tggatgttgc cgttgtgaat caactccgaa
    44941 atcaggggcg aattgagcag cacctcgact gccgccaccc tgcccttgcc gccgtctcgc
    45001 ggaacgaggc gttgcgaaat aaacgcctga aggttgagcg acaaatccgt cagcaattgt
    45061 tcgcgccgct cctcggggaa aaagttgatg atgcggtcga gtgcctgatt ggtgctgttg
    45121 gcgtgcagcg tcgccataca caaatgcccc gtttcggcaa aggcaatggc gtagtccatc
    45181 gtttcgcggt cgcggatttc gccgataagg atgacatccg gcgcctgacg cagcgtgttt
    45241 ttcaacgccg ccatccagtt ttccgtatcc acgccgacct cgcgctgggt gatgatgcag
    45301 tttttgtgtt cgtggacaaa ctcgatcggg tcttcgatgg tgatgatatg tccgaacgaa
    45361 ttttcattgc ggtaatcgat aagcgaggca agcgaagtcg atttgcccga gccggtgccg
    45421 ccgacaaaaa taaccagccc gcgttttttc agcgcgacat ccttcaagac cggcggcagg
    45481 ttcaggcttt caaacttggg aatcttgctg gtaatcgcac ggaataccaa cgccgtcgcg
    45541 ccgcgctgta tcatcgcatt gacgcggaag cggctggtgt ccggcaggct gatggcgaag
    45601 ttgcactcgt tggtcgatga aaattcttcc gcctgcttcg cactcataat cgaaaaggcg
    45661 atttccatac atttttccgc cgtcagcggt tcgtccgtga tgcgggtgat tttgccgtcc
    45721 agcttcattg cgggcgggaa atgggtcgtc acaaacaggt cggaaccttt gtttttgttc
    45781 atatggcgca gccatgcgaa cagttcttcc tttgcggtca tattttcatc ggtaaacatc
    45841 ctcgtcccct aatcttcaaa caatctgtac cttcccattc taccaaaaac ccgccgtgcc
    45901 gtctgaaacc gtttcagacg gcacggacaa accacctcca ctcaatcctg ccgtgttagc
    45961 gtgtataatc aggcattcta tccgaaattc acaacaggtc agcaatatgt ctttgaaaac
    46021 agtagccgtt atcggcgcaa tggaacaaga aatcgagctt ttgcgcgaga tgatggaaaa
    46081 tgtcaaagcc gtctctttcg gcaagtttgc cgcctatgaa ggcgaattag cgggaaaacg
    46141 catggtgctt gcattgagcg gcatcggcaa ggtcaacgcg gcggttgcaa cggcttggct
    46201 tatccatcaa ttcgcaccgg actgcgttat caacaccggc agcgcgggcg gcttgggcaa
    46261 gggtttgaaa gtcggcgatg tcgtggtcgg cacagaaatc gcgcaccacg atgtcgatgt
    46321 aaccgcattc ggctatgttt ggggacaagt gccgcaactg cccgccgtat ttgtttcaga
    46381 cggcctcttg gtcggcaaag caaaacaggc ggcggaagtg tttgaaggcg cggcggtaga
    46441 acaaggcctg attgtcagcg gcgaccgctt cgtccacagc agcgaaggcg tggcggaaat
    46501 ccgcaagcac ttccccaaag tcaaagcagt ggaaatggaa gcggcggcga ttgcccaaac
    46561 ctgtcatcag ttggaaacgc ctttcgtcat catccgcgcg gtttccgatt cggcggacga
    46621 aaaggcagac atcagctttg acgaattttt gaaaacggcg gcggcaagtt ctgcaaaaat
    46681 ggtggcagaa atcgtcaaat ctttataaaa cctttatcaa atcatgccaa aacagcggtt
    46741 ttccgttaga atatcgggct atttccacgt tgcacccgat attcggacgc aacgccgttt
    46801 gttcagacgg catttcccaa acccactgtc cgacattcaa aacaactcta atgaaaaata
    46861 tccgaaattt ctccatcatt gcccacatcg accacggcaa atcgacgctt gccgaccgct
    46921 tcatccaata ttgcggcggt ttggatttgc gcgaaatgag tacgcaggta ctcgattcca
    46981 tggacatcga aaaagagcgc ggcattacca tcaaagcgca aaccgccgcg ctcaactata
    47041 aagcgcgcga cgggcaggtg tatcagctta atttgattga cacgccggga cacgtcgact
    47101 tctcttacga agtttcccgt tcgctgtcgg cttgcgaagg cgcgcttttg gtcgttgacg
    47161 cgtcgcaggg cgtggaagcg caaaccgtag cgaactgtta caccgcgatt gatttgggcg
    47221 tggaggttgt gcctgttttg aacaaaatcg acctgccagc cgccgacccc gaacgcgtgg
    47281 agcaggaaat tgaagacatc atcggcatcg atgcggtcgg cgcggtgcag tgttccgcca
    47341 aaagcggcat cggcgtggaa gacgttttgg aagaaatcgt tgccaaaatt cctgcgccga
    47401 ccggcgatga aaacgcgccg ctgcaagccg tgattgtcga ttcgtggttt gacaactacg
    47461 tcggcgtggt catgctgatt cgagtgaaaa acggcaccat caaactgaaa gacaaagtgc
    47521 gctttatgag caccaaggcg gaaacgcagg ttgagcagtt gggcgtattc acaccgaaat
    47581 cggttcaaaa acaagaactc aaagccggcg aagtgggctt tttgattacc ggcgtaaaag
    47641 aattgggtca ggcgaaagtc ggcgatacgg ttacactggt tgccaaccct gccactgagc
    47701 cgctgcccgg cttccaagaa gtacaaagcc aagtattcgc cggactttat cccgtggaaa
    47761 gccacgacta cgaagccttg cgcgacgctt tggaaaaatt gcagcttaac gacgcttcgt
    47821 tgaaattcga gcctgaagtt tcccaggcat tgggcttcgg cttccgttgc ggcttcttgg
    47881 gtctgttgca cttggaaatc gtgcaggaac gcttggagcg cgagttcgat atggatttga
    47941 ttaccaccgc gccgacggtg gtttacgaag tcgtgttgaa aagcggcgaa aaaatcgaag
    48001 ttgaaaaccc gtccaaactg cccgacatcg gcagcatcga aaccatactc gagccgatta
    48061 ttaccgcgac catccttgtg ccgcaggaat acgtcggcaa cgtcatgact ttgtgtaacc
    48121 aaaagcgcgg cgttcaggtc aatatgcagt acatgggccg ccaagtcatg ctgacttacg
    48181 atttgcccat gaacgaagtc gtcatggact tcttcgacaa actcaaatcc acttcgcgcg
    48241 gttatgcttc gttggactac catttcaaag agttccaacc gtctgatttg attaagctgg
    48301 atattatggt caacggcgaa aaagtcgatg cattaagcct gattgtgcac cgtcaaagcg
    48361 cggttcaccg aggccgcgag ctggcatcaa aaatgcgcga gctgattccg cgccaaatgt
    48421 tcgacatcgc cgtccaagcc gccatcggca gccagattat cgcccgcgaa aacgtcaaag
    48481 ccctgcgtaa aaacgttttg gcgaaatgtt acggcggcga tattacgcgt aagaaaaaac
    48541 tgcttgaaaa acaaaaggca ggtaaacgcc gtatgaaaca agtgggcaat gtggaaattc
    48601 cgcaaagcgc gttcttggcg attttgcagg tgagcgacaa ataactctgg cacacagggc
    48661 ggcgcgtcgg gaacacccct caaaccgccc tgatttcagg ggcacgcgcc cgcaaacaaa
    48721 ttttgcaatt tgccgcctta aaacggttgc acggacaggc gggtttttaa gcattaagga
    48781 acaacaatga acacaatgct aatgtcgggc gcggctgccg cgctgcttgc cggcatcatc
    48841 ctttatttca aaagcgacaa aaagcggcag gaaaacggtg aatggagttc cggtctggaa
    48901 tacgcctata tcctgacggc ggtcggcgtg tttgccgctt tgtccctgtt tatgagcttt
    48961 accgccgttt tcctgatttt cgttgtattg tgcggtacgg cttggggggt atacaaatac
    49021 cgcctgaaga ctcatcccga aatctcggaa agcagccact tcggcgatta tttcggcagt
    49081 ttcttcccta ccgttttggt attgttcctc atccggtcgt ttattgccga accgttccaa
    49141 atcccgtcca gctcgatgcg cccgggcctg attaagggcg atttcatttt ggtcggcaaa
    49201 ttttcctacg gcctgcgcgt acccgtttta aacaatatat ttattcctac aggcaaaatc
    49261 gaacggggcg atgtggtcgt ttttaattat cctttgcaac cggagatgac ctacatcaag
    49321 cgtattgtcg gcattccggg cgatgtggtc gaatatcgga ataaagtttt gacggtaaac
    49381 ggaaaacctg cttccgatat tcctgacggc acataccgtt atcccgacga caccgaccca
    49441 tccgaaatcc acaatacgga tatgttccac agcggtttgg acggcaaatc cttcaatatt
    49501 ctgaaaaaag aaggacagcc tgccgtttcc ctgcccgtat tgggcaaata tacctccgat
    49561 attatgtctg aaaacggata ttccatagag caaagcggtt tggaacactg ccaatatgcc
    49621 gacgacggca gcggtttcgt gtgcaaagtt cccgaaggac gctatttcgc tatgggcgac
    49681 aaccgcgaca acagtgccga ttcgcgctac tggggatttg tggatgacaa gctggttgtc
    49741 ggcaaggcaa tgttcatttt gatgaacttc ggcgatttcg gcagggccgg tacggcaatc
    49801 cgttaggccg gaaaattacg ccggaacatc aaaaatgcca tccgaaccct gtttcagacg
    49861 gcatttttat catcaaacgc cttgtttccc tgccggcagc ctcggggata atatagtgga
    49921 ttaaatttaa tccactatat ttccgtttgt gtggaaaccc atcccattga ataaagggca
    49981 tttcgtccga ctgacggtgt gggcggcata tggaaatacg gacattattt tagttgcatt
    50041 gccattccag cggaggcggc ttttttgatt ctgcccgccc cgatgccgtc tgaaagggct
    50101 tcagacggca ttttccgctc cgcgccgttg ctggcgtgtt tggtcttgcc ggttttgact
    50161 gcctgccagt tcaatcgtac gcaggtttgc cacggcagaa aatctgatgc cggacggagt
    50221 caaggctcgg actgctgttt cagcgaaccg aggtaaattg ccgaaagcgt cagcgccacc
    50281 cctgcccact gcacgccttc aatcggtttg cccaacccga aataatcgat gaacagggcg
    50341 gcaaccggtt cggacaaaag cagcagcccc gtcagcgaca gcgaaagcag cggaatcgca
    50401 taggcaacca tcgcccacgc gaagcactgc atcaccacgc cgtacacaag caccaaaccc
    50461 gcatctttcc aagtcgtcgg ataaagcgcg ccgccatcca tcagcaatgc cggaacaacc
    50521 agcgataccg cgccgcccaa actcaaaatc atcattgaag ggaaaagcgc caccgtctcg
    50581 atttcatggg ttttgcggac gaacaccatc gacagggcga gcatcagtcc cgataccaaa
    50641 ccgctgacga atccccaaac cgcattaccg ttgtagccga attccgcacc ggcaatcatc
    50701 gccacgccgg caactgccga tattaagcct gcctttttca gcccgctcaa acgctcgccg
    50761 aaaaagaaaa caccgattgc cgacaagaaa aagatttgca ggctgttgag cagggtggaa
    50821 atacccggcc cgaccgcgtg tatgctttcg tgccacaacg ccaaatcgaa agcgagaaac
    50881 acgcccgccg tcagggcata tcggacggtt ttcctgtttt ttgggaattt ttgcctgaaa
    50941 aaccgtgcta aaaaccaaaa tacgaacacc gaaatcagca accgccaaaa tgcgattgca
    51001 tacgaaccga cggggacgga tctgacaatc agactgccta gaccgaacac cacgcaaccc
    51061 agcaccagca atggtgcggc atattttttg ccgttaccga ccacaatatc ccctcccttc
    51121 cccatacggc tgccgcgccg acggcaagaa tgccgccatt ccgaaaatat gccgtctgaa
    51181 gcccaaagcc ccggctgcac ccggacaaac ggctgatttt acgggcaggc cgactatttt
    51241 ccgttggggc aaaaaaccgt cctgccggat aaaaccc
//
LOCUS       DAXHPG010000002        35148 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXHPG010000002 DAXHPG010000000
VERSION     DAXHPG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.002"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            81..1046
                     /locus_tag="ACFM6F_000057"
     CDS             81..1046
                     /locus_tag="ACFM6F_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6163561.1"
                     /translation="MLRLTALAVCTALALGACSPQNSDSAPQAKEQAVSAAQTEGASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESSKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGSSSRLGGWL
                     HKDIGVPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKEQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1064..1240
                     /locus_tag="ACFM6F_000058"
     CDS             1064..1240
                     /locus_tag="ACFM6F_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163562.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFYWNLPYPLPDTEA
                     SNKAFRSDGSDCLV"
     gene            1275..2243
                     /locus_tag="ACFM6F_000059"
     CDS             1275..2243
                     /locus_tag="ACFM6F_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6163563.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFVGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2233..3207
                     /locus_tag="ACFM6F_000060"
     CDS             2233..3207
                     /locus_tag="ACFM6F_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC6163564.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFLTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPAVGKFGFELVVMMGGSLLLFYTLVKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFMAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3252..3878
                     /locus_tag="ACFM6F_000061"
     CDS             3252..3878
                     /locus_tag="ACFM6F_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC6163565.1"
                     /translation="MTQERFPSFFNQAPTITVRDALAEFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPE
                     MRELMPKAVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3962..4720
                     /locus_tag="ACFM6F_000062"
     CDS             3962..4720
                     /locus_tag="ACFM6F_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6163566.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLACTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPDDIFTAANIKTLFDMDVSVLDHQSKKLIVHHI"
     gene            complement(4779..5117)
                     /locus_tag="ACFM6F_000063"
     CDS             complement(4779..5117)
                     /locus_tag="ACFM6F_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC6163567.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            5212..5424
                     /locus_tag="ACFM6F_000064"
     CDS             5212..5424
                     /locus_tag="ACFM6F_000064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163568.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRTETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            5582..9568
                     /gene="purL"
                     /locus_tag="ACFM6F_000065"
     CDS             5582..9568
                     /gene="purL"
                     /locus_tag="ACFM6F_000065"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC6163569.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDRQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQAYGKPDHIASPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDAFRAI
                     CERERCPFAVVGTATGDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPYAT
                     EGNPFGKKPFNAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQ
                     TPVADCAVTMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCIGEAITNIAAVNIGDIG
                     NIKLSANWMAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGADK
                     KSVVSPLSLIISAFAPVEDVRKTVTPELKNVEDSVLLFIDLGFGKARMGGSAFGQVYN
                     NMSGDAPDLDDTSRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLD
                     IDLNLLLAQTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVA
                     DIINLFYQQQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRL
                     RDNPACADSEFALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQ
                     IEMAAAFTRAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSIL
                     FHPALRDQFAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARL
                     SMVHVPKSASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQV
                     TQTYPLNPNGSPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLF
                     AGARKALG"
     gene            9663..10421
                     /gene="gloB"
                     /locus_tag="ACFM6F_000066"
     CDS             9663..10421
                     /gene="gloB"
                     /locus_tag="ACFM6F_000066"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC6163570.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            10735..15210
                     /gene="mspA"
                     /locus_tag="ACFM6F_000067"
                     /gene_synonym="ausI"
     CDS             10735..15210
                     /gene="mspA"
                     /locus_tag="ACFM6F_000067"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HFC6163571.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIVRNDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLIHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGDSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMENSTSWQFIGNNRQQAAKQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNGPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPAPTSHGMPTA
                     PKTGNFSDMTGEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYDLSGIDLGFIQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPAT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPLKLEGDSRGAFQI
                     HVKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNP
                     LKEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARI
                     DSQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFETELD
                     TYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNL
                     AELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHR
                     PYQQTTNYAHISIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENG
                     ALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSN
                     GNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYDTRQNSGSALS
                     VNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            15442..16896
                     /gene="mgtE"
                     /locus_tag="ACFM6F_000068"
     CDS             15442..16896
                     /gene="mgtE"
                     /locus_tag="ACFM6F_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC6163572.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(16982..17890)
                     /gene="hslO"
                     /locus_tag="ACFM6F_000069"
     CDS             complement(16982..17890)
                     /gene="hslO"
                     /locus_tag="ACFM6F_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC6163573.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            18135..18890
                     /locus_tag="ACFM6F_000070"
     CDS             18135..18890
                     /locus_tag="ACFM6F_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6163574.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            18909..19127
                     /locus_tag="ACFM6F_000071"
     CDS             18909..19127
                     /locus_tag="ACFM6F_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163575.1"
                     /translation="MSMPEIPKWYDDDGQIVSCTEKIKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            19272..19616
                     /locus_tag="ACFM6F_000072"
     CDS             19272..19616
                     /locus_tag="ACFM6F_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC6163576.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            19616..20308
                     /locus_tag="ACFM6F_000073"
     CDS             19616..20308
                     /locus_tag="ACFM6F_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC6163577.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            20375..21595
                     /gene="argJ"
                     /locus_tag="ACFM6F_000074"
     CDS             20375..21595
                     /gene="argJ"
                     /locus_tag="ACFM6F_000074"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC6163578.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLSFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            21655..23064
                     /locus_tag="ACFM6F_000075"
     CDS             21655..23064
                     /locus_tag="ACFM6F_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6163579.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETTAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            23254..24645
                     /gene="hrpA"
                     /locus_tag="ACFM6F_000076"
     CDS             23254..24645
                     /gene="hrpA"
                     /locus_tag="ACFM6F_000076"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC6163580.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLIGKDEDDAEIELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEENFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            24746..25873
                     /locus_tag="ACFM6F_000077"
     CDS             24746..25873
                     /locus_tag="ACFM6F_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFC6163581.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            26050..27627
                     /gene="lpt3"
                     /locus_tag="ACFM6F_000078"
     CDS             26050..27627
                     /gene="lpt3"
                     /locus_tag="ACFM6F_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC6163582.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKTSRLLIALFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRHKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSRIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            27729..30935
                     /locus_tag="ACFM6F_000079"
     CDS             27729..30935
                     /locus_tag="ACFM6F_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC6163583.1"
                     /translation="MDARSNPANVSDGLQNSSSHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAASKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRSPEVRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNVYTSTRGSRFHLFPASALFKAKPKWVMAAELVETTKLYARDVAAIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVIASERVTLYGLTVLPRRPVSYGRIAPEEAR
                     EIFIRGALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYATQQPTPSPEREGRGEGKTVAAQTNFSATAANPLPQERE
                     QSASASTFSDDLRPANLQQTAPSPVGEGWGESKTVATQTNFSATSTLSDDPKPKKQPA
                     PQKGRLKPLPLADIRTFQAWLKTAECDNPRLLFLSRDDLMQHAAAHITEEQFPKFWQT
                     ADGKFKLSYRFEPHHPLDGVTMTVPLTVLNRLHAPSLEWLVPGMLREKIQLLIKALPK
                     QIRRICVPVPDFITKFLESNPDRQATIIPQLAHFIAKSASDMRILEQIDQDAWAAQEL
                     PEHCYLNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNTQEFERDNVTTWDIGT
                     LPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQ
                     VKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDELPRNEKAFKEQIKR
                     ARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPGFATRTPWA
                     QWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKNDGLVKQGQPISDNL
                     AAFKWMIEELRVSLFAQELKTPYPVSVKRLLKEWEKIF"
     gene            31002..31832
                     /locus_tag="ACFM6F_000080"
     CDS             31002..31832
                     /locus_tag="ACFM6F_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6163584.1"
                     /translation="MSLENFQLIKTQLGVPSYENDRVAIYQGDCIQLMDKISKGSFQM
                     TITSPPYNIGKEYESILDLEHYLEWCERWMQSIHHLTEDSGCFWLNLGYLHVEKKGLA
                     VPIPYLLWDKTNFFLLQEVVWNYAAGVACRNRFSPRNEKLLWYVKNPAKYTFNLDAVR
                     DPNVKYPNQKKNGKLKCNPLGKNPTDVWQIAKVTSGRNRSSRERTAHPAQFPLELIER
                     IIRASSNQNDVILDPFIGSGTTALAGLKNNRFVIGFEINDEYIDIIKERFKEYDLQNT
                     "
     gene            complement(31889..32488)
                     /locus_tag="ACFM6F_000081"
     CDS             complement(31889..32488)
                     /locus_tag="ACFM6F_000081"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScaI family restriction endonuclease"
                     /protein_id="HFC6163585.1"
                     /translation="MNRSKISPYASLNEELWISKTEELIKQHPLSEKEIVQLCLDSWE
                     NIFSSRIGNLQIGKNFFPSPQIVGALLHALIPANIEARFENWQGEKNKYDKDIVYIKD
                     DYYSIELKTSSDPKYIFGNRSYAQPQDGITGKSKNGYYLTINFEKFMPDLKLRPEIKI
                     IRFGWLDHTDWIAQTAATGQQARLSPEAYRSKLKILYQK"
     gene            complement(32490..32705)
                     /locus_tag="ACFM6F_000082"
     CDS             complement(32490..32705)
                     /locus_tag="ACFM6F_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6163586.1"
                     /translation="MENELVEFGKRVKHYRLLNGWSQEELAEKVGLHRTYIGSVERGE
                     RNISLLNISLLAKSFSINISELVEGIK"
     gene            complement(33137..33604)
                     /locus_tag="ACFM6F_000083"
                     /pseudo
     CDS             complement(33137..33604)
                     /locus_tag="ACFM6F_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            33836..34048
                     /locus_tag="ACFM6F_000084"
     CDS             33836..34048
                     /locus_tag="ACFM6F_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HFC6163587.1"
                     /translation="MPHIHPLHLFPPHIAERGLLYFQQGKVGAVQKTSTGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            34232..34500
                     /locus_tag="ACFM6F_000085"
                     /pseudo
     CDS             34232..34500
                     /locus_tag="ACFM6F_000085"
                     /inference="COORDINATES: protein motif:HMM:NF017953.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(34590..35039)
                     /locus_tag="ACFM6F_000086"
     CDS             complement(34590..35039)
                     /locus_tag="ACFM6F_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6163588.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         8866 a        10386 c         8394 g         7502 t
ORIGIN      
        1 ttatcattta tcctttctaa agccgttccg gtttgtccga ccggcggctt tgccccaata
       61 tccccatttt ggagacacct atgttacgtt tgactgcttt agccgtatgc accgccctcg
      121 ctttgggcgc gtgttcgccg caaaattccg actctgcccc acaagccaaa gaacaggcgg
      181 tttccgccgc acaaaccgaa ggcgcgtccg ttaccgtcaa aaccgcgcgc ggcgacgttc
      241 aaataccgca aaaccccgaa cgcatcgccg tttacgattt gggtatgctc gacaccttga
      301 gcaaactggg cgtgaaaacc ggtttgtccg tcgataaaaa ccgcctgccg tatttagagg
      361 aatatttcaa aacgacaaaa cctgccggca cgctgttcga gccggattac gaaacgctca
      421 atgcctacaa gccgcagctc atcatcatcg gcagccgcgc agccaaagcg tttgacaaat
      481 tgaacgaaat cgcgccgacc atcgaaatga ccgccgatac cgccaacctc aaagaaagct
      541 caaaagagcg tatcgacgcg ctggcgcaaa tcttcggcaa acaggcggaa gccgacaagc
      601 tgaaggcgga aatcgacgcg tcttttgaag ccgcgaaaac tgccgcgcaa ggcaaaggca
      661 aaggtttggt gattttggtc aatggcggca agatgtccgc cttcggctcg tcttcacgac
      721 tgggcggctg gctgcacaaa gacatcggcg ttcccgctgt tgacgaagcc atcaaagaag
      781 gcagccacgg tcagcctatc agctttgaat acctgaaaga gaaaaatccc gactggctgt
      841 tcgtccttga ccgcagcgcg gccatcggcg aagagggtca ggcggcgaaa gacgtgttgg
      901 ataatccgct ggttgccgaa acaaccgctt ggaaaaaaga acaggtggtt tacctcgttc
      961 ccgaaactta tttggcagcc ggtggcgcgc aagagctgct gaatgcaagc aaacaggttg
     1021 cggatgcgtt caacgcggca aaataatgaa acggcggcat tcgatgccgt ctgaaacacg
     1081 gatgcaaacc gcttcctgtg tttcagacgg cattgcccga tacgggcgtt atcgcgggaa
     1141 tggcggcaag cgtttttatt ggaacttgcc ctatcctctg cccgatacgg aggcttcaaa
     1201 caaggctttc cgctccgacg gttcggactg ccttgtttga atcttctacg ccttaacgct
     1261 tttcctttct gtttatgact gccaaacctt tttccctcaa cctgaccaac ctcctgctgc
     1321 tggcggtatt gtttgccgtc agcctgtcgg tcggcgttgc cgatttccgc tggtcggatg
     1381 tgttttcgct gtccgacagc cagcaggtta tgttcatcag ccgcctgccg cgcacgtttg
     1441 cgattgtgct gacgggcgcg tcgatggcgg tggcggggat gattatgcag attctgatgc
     1501 gtaaccgttt tgtcgagcct tctatggcgg gcgcgggtca gagtgcggct ttgggtttgc
     1561 ttctgatgtc cctgctgctg cctgccgcgc cgctgccggt caaaatgtcg gttgccgccg
     1621 ttgccgcgtt aatcgggatg ttggtgttta tgatgcttat ccgccgcctg ccgccgacgg
     1681 cgcaactgat ggtgcctttg gtcgggatta ttttcggcgg cgtggttgag gcggtggcca
     1741 cctttattgc gtatgaaaac gaaatgctgc aaatgctggg cgtgtggcaa cagggcgatt
     1801 tttcgagcgt gctgctgggg cggtacgagc tgctttggat tacgggcggt ttggcggtgt
     1861 ttgcctatct gattgccgac cggctgacga ttttggggct gggcgaaacg gtcagcgtga
     1921 atttgggttt gaaccggacg gcggtgttgt ggtcggggct gattattgtg gctttgatta
     1981 cgtcgctggt tatcgttacg gtcggcaata ttccgtttgt cgggctggtc gtgccgaaca
     2041 tcatcagccg cctgataggc gacaggctgc gccaaagcct gcctgcggtg gccttgttgg
     2101 gcgcgtcttt ggttttattg tgcgacatta tcggacgcgt gattgtgttt ccgtttgaaa
     2161 ttccggtatc gaccgtcttc ggcgtattgg gtacggcgtt gtttttatgg cttttgttaa
     2221 ggaaacctgc tcatgccgtc tgaaaaaaat atcggtttta tggcaggaag cagccgtccg
     2281 ttgtgggttg cctttgcgct gttgctggtt tcctgcgtcc tgtttttaac gctcaacgtc
     2341 aaaggcgatt gggactttgt actgcacctg cgtctgacca aacttgccgc gctgctgatg
     2401 gtcgcctatg cggtcggcgt gtccacgcaa ctcttccaaa cgctgaccaa caatccgatt
     2461 ctgacccctt cgattttggg tttcgattcg ctgtatgtgt ttttgcagac cttgctggtg
     2521 tttacgttcg gcggcgtggg ctatacgtcc ctgcctgcgg tcggcaagtt cggctttgaa
     2581 ttggtcgtta tgatgggcgg ctcgctgctg ttgttctaca cgctcgtcaa acaaggcggg
     2641 cgcgatttgt cgcgcatgat tttaatcggc gtgattttcg ggattttgtt ccgcagcctg
     2701 tcgtcgctgc tttcgcgcat gatcgacccc gaagaattta tggcggcgca ggcgaatatg
     2761 tttgccggat tcaataccgt ccacagcgag cttttgggca taggcgcgct gattctgctc
     2821 gtcagcgcgg cggtcgtttg gcgcgaacgc taccgcttgg acgtacacct tttggggcgc
     2881 gaccaagccg tcaatttggg catcagctac acgcgcaaca ccttatggat actgctttgg
     2941 attgccgcat tggtggcgac ggcgaccgcc gttgtcggcc cggtaagctt tttcgggctt
     3001 ctcgccgcct cgcttgccaa ccacttttcc ccgtccgtgc gccattccgt ccgcctgccg
     3061 atgacggttt gtgtcggcgg catcctcctg gtcggcggac aaaccgtatt cgaacacttc
     3121 ttgggcatga aggcggtatt aagcgtggtg gtcgaatttg cgggcggact cgttttcctc
     3181 tatctcgttt taaaacacaa aaaatgacgg atgccgtctg aacggcagcc tggctcgaaa
     3241 ggacaaacca tatgacgcaa gaacgtttcc catcattctt caaccaagcc ccgaccatca
     3301 ccgtcaggga cgcgcttgct gaattcctcg gcgcggccga aaatggcatc ctcacttacc
     3361 gctacgccga tgccgtgcgc ctgtgcggcc actcctgccc gaccgtcgcc ggcgcgtacc
     3421 tgatggttat caaaggtctg aaagcccttt acggcgaaga gctgcccgaa cgcggcggca
     3481 tcgaagccgc catgcaggga gcgcgcgacg aaggcacggt cggcgtaacc gcgtccgtcg
     3541 tccaactcct caccggcgca gccccggaaa ccggcttcgg aggcatcgga atgcagggac
     3601 gcttcgcccg ccgtaacctc ttatcctttg gtgcaggcga aatcaacggc acactgaccc
     3661 tgcgccgcaa agacaacggc aaaaccgtcg ccgtcagcct caacgccgcc ctacaaccct
     3721 ttgcacccga aatgcgcgaa cttatgccca aagccgtcgg cggcagcgca agcgcagaag
     3781 aactcgaacg cttcggacaa ctctggcagg cacgcgttaa agcatttttg accgaatcgg
     3841 cggacgaccc gcagttcgtc atcgtccgcg aagtgtgagc gttcagacgg cattccgaat
     3901 ttcaaatgcc gtctgaaccc ccgccaaaca acaacaaacc tacgcccgac aagcatccgc
     3961 catgattacc atccgcaacg tcagctaccg catcggcaca cgccccatcc tcgacaacgt
     4021 cagcctcgac atccccgaag gcggcattac cgccctcgtc ggccccaacg gtgcgggcaa
     4081 atccaccctg ttttccttta tggcgcggct gcgcccgctt gaaagcggca gcatcgccta
     4141 ccgcggcaaa aaccttgcct gcacccccac cgccgaactc gccaaaaccc tgtccatcct
     4201 cacccaagaa aacagcatca tgagccgcat caccgtgcgc gacctgctca tgttcggccg
     4261 ctatccctac catcaaggca gaccgactgc cgaatgccgc cgtatcgtta acggtgcaat
     4321 cgaagaattc cacctgcaag acttcgccga ccgctacctg accgagcttt ccggcggaca
     4381 acgccaacgc gccatgattg ccatggtgtt ctgccagcgc accgactacg ttttgctgga
     4441 cgaaccgctg aacaacctcg acatgtatca tgcccgctcc ctcatgcaaa tcctgcgccg
     4501 gctgaccgac gaacacaagc gcaccaccgt cgtcgtatta cacgacatca accaggccgc
     4561 cgcctacgcc gaccacgtcg tcgccatgaa aaacggccaa gtcgccatgc agggcaagcc
     4621 cgacgacatt ttcaccgccg caaacatcaa aaccctgttc gatatggatg tgtccgtcct
     4681 cgaccaccaa agcaaaaaac tcatcgtcca ccatatctga agctcaaaaa gaaaatgccg
     4741 tctgaaaacc tttcagacgg catttccatt ccaaacgctc aaaccgccgc gtccgcacgt
     4801 tcgcccgtgc ggatacggat tgcctcctcg accggcagca caaaaatctt gccgtcgccg
     4861 atttttcccg aacgcgccac ctcgacaatc acgtcaatcg cgcgttccac agcatcatcc
     4921 gccaacacca gctcgatttt gactttgggc aggaaatcga cggcgtattc cgcaccgcga
     4981 tagatttccg tatgcccctt ctgcctgccg aaccctttga cctcgctgac ggtcatgccc
     5041 gtaatgccga tttccgtcaa cgcctcgcgc acgtcgtcga gtttgaacgg tttgacaatc
     5101 gcctcgattt ttttcatcac attctccatt aattaacgta aacaaacgac aaatatagca
     5161 gaaaccgccg gcaaacgata aacggtactg ttcgcccggc gatgcggcga tttgccggaa
     5221 accgtcattc ccgcgcaggc gggaatcccg atctctgcat ttcagcaata tttaaaggtt
     5281 tctgtaaatc caaacttcca tattcctaac cgtacggaaa cgacggcatt tgatctaaaa
     5341 acatacggaa gcagttgccc gcccctctac ttcctgcaat tatccgcata cccggctttg
     5401 ggtaaaaatc agaaaaacag ataaaaccct tattccttaa tatcttcaga cggcacctgt
     5461 attttcccca actgtatgcc gtctgaagat tgtcaacatt ttaagccaaa atatccaagc
     5521 aatacaaacc tgtccggcgt ataatccctc tttttcccaa ccgcatttga gagccgaatc
     5581 catgtctgtc gttttgccct tgcgcggcgt taccgccctt tccgatttcc gtgtcgaaaa
     5641 actcttgcaa aaagccgccg cactcggact gcccgaagtc aaattaagca gcgaattttg
     5701 gtatttcgcc ggcagcgaga aagcacttga tgccgcgact gtcgaaaaac tgcaagcctt
     5761 gttggcggcg caaagcgttg aacaaacgcc aaaagcgcgc gagggcttgc atttgttttt
     5821 ggtcacgccc cgtttgggta cgatttcgcc gtgggcttcc aaggcgacca atatcgcgga
     5881 aaactgcggt ttggcaggca tagagcgcat cgagcgcggt atggcggtgt ggctggaagg
     5941 cgcgcttacc gacaggcaaa aacaacaatg ggcggctttg ctgcacgacc gcatgaccga
     6001 atccgtattg cccgattttc agacggcatc caaattattc caccatctcg aatccgaaac
     6061 cttctccacc gtcgatgttt tgggcggcgg taaagaggct ttggtcaaag ccaataccga
     6121 aatgggtttg gcactctccg ccgacgaaat cgattatctg gttgaaaact atcaggcttt
     6181 gcagcgcaat ccgtccgatg tggagctgat gatgttcgcg caggcaaaca gcgaacactg
     6241 ccgccacaaa atcttcaacg ccgatttcat cctcaacggc gaaaaacaac cgaaatccct
     6301 gttcggcatg atacgcgaca cgcacaacgc gcatcccgaa ggtacggtcg tcgcctataa
     6361 agacaattcg tccgtgatcg aaggcgcgaa aatcgagcgt ttctatccga atgcggcgga
     6421 aaaccaaggc taccgtttcc acgaagaaga cacgcatatc atcatgaaag tggaaacgca
     6481 caaccacccg accgccatcg cgccgtttgc gggcgcggcg acgggtgcgg gcggcgaaat
     6541 ccgcgacgaa ggcgcgacgg gcaaaggttc gcgcccgaaa gcaggcttga ccggctttac
     6601 cgtctccaac ctcaacatcc ccgacctcaa acagccttgg gaacaagcct acggcaaacc
     6661 cgaccatatt gcttcccctt tagacatcat gatcgaaggc cccatcggtg gcgcggcgtt
     6721 caacaacgaa ttcggacgcc ccaacctctt gggctacttc cgcacctttg aagaaaaatt
     6781 tgacggtcag gttcgcggct accacaaacc gattatgatt gcgggcggct tgggcagcat
     6841 tcaggcgcag cagacgcata aagacgaaat ccccgaaggc gcgttgctga tccaactggg
     6901 cggcccgggt atgcttatcg gcttgggcgg cggcgcggct tcttcgatgg ataccggtac
     6961 aaacgacgct tctttagact tcaactccgt gcaacgcggc aaccccgaaa tcgaacgccg
     7021 cgcgcaggaa gtcatcgacc gctgctggca gctcggcgac aaaaacccga ttatctccat
     7081 ccacgacgtg ggcgcaggag gtttgtccaa cgccttcccc gaactcgtca acgatgccgg
     7141 acgcggcgcg gtattcaagt tgcgcgaagt gccgttggaa gaacacggtt taacgccttt
     7201 gcaaatctgg tgcaacgaat cgcaagagcg ttatgtgttg tcgattttgg aaaaagattt
     7261 ggatgccttc cgcgccatct gcgaacgcga acgctgcccg tttgccgtag tcggcacggc
     7321 gaccggcgac ggccatttga aagtacgcga cgatttgttc tccaacaacc ctgtcgattt
     7381 gccgttgaac gttttgctcg gcaaaccgcc caaaaccacg cgcaccgaca aaacggttac
     7441 gccgtatgcg accgaaggga atccctttgg gaaaaaaccg tttaatgccg gcgatatcga
     7501 cattaccgaa gccgcctacc gcgttctgcg cctgcctgcc gtagcggcta aaaacttcct
     7561 gattaccatc ggcgaccgca gcgtcggcgg catgacccac cgcgaccaaa tggtcggcaa
     7621 ataccaaacc cccgtagccg actgcgccgt gaccatgatg ggcttcaaca cctatcgcgg
     7681 cgaagcgatg tctatgggcg aaaaaccggc cgtcgccctg tttgacgcgc ctgcttcggg
     7741 cagaatgtgt atcggcgaag ccatcaccaa catcgcagcg gtcaacatcg gcgacatcgg
     7801 caatatcaaa ctttctgcca actggatggc ggcgtgcggc aacgaaggcg aagacgaaaa
     7861 actctaccgc accgtcgaag cggtttccaa agcctgtcag gcattggatt tgagcatccc
     7921 cgtgggcaaa gacagcctgt cgatgaaaac cgtttggcag gatggcgcgg acaagaaatc
     7981 cgtcgtctcg ccattgagcc tgattatctc cgcgttcgcg ccggtggaag acgtacgcaa
     8041 aaccgttacg cccgaactga aaaacgtcga agacagcgta ttgctgttta tcgatttggg
     8101 cttcggcaaa gcgcgtatgg gcggctcggc gtttggtcag gtgtacaaca atatgagcgg
     8161 cgacgcgccc gatttggacg atacaagtcg tctgaaagcg ttttacaacg tgattcagca
     8221 gcttgtcgcc gaagacaaac tcttggcgta tcacgaccgc agcgacggcg gtttgtttgc
     8281 cgttttggta gaaatggcgt ttgcggggcg gtgcggcttg gatatagatt taaatttatt
     8341 gcttgcacaa acatttatta ccaaccatac cgctctgtct caatcattgc ggactgaaga
     8401 ggtaaaagcg ttggctgaat ggcaagaaac cattgcccgc acattattta atgaagagtt
     8461 gggtgctgtt atccaagtta gaaaacaaga tgttgccgat attatcaatt tattctatca
     8521 acaacagctg catcataatg tctttgaaat cggtacgtta actgatgaga acacgttaat
     8581 catccgcgac gggcaaacgc accttatttc tgacaaccta atcaaactgc aacaaacctg
     8641 gcaagaaacc agccatcaaa tccaacgcct gcgcgacaac cctgcctgcg ccgacagcga
     8701 gtttgccctg attggcgaca acgaacgcag cgcattgttt gccgacgtga agttcgacgt
     8761 gaacgaagac atcgccgcgc cgtttatcaa cagcggcgcg aaacccaaaa tcgccatcct
     8821 gcgcgaacag ggcgtaaacg ggcaaatcga aatggccgcc gcgttcactc gcgccggttt
     8881 cgatgcctac gacgtgcata tgtccgacct gatggcaggc cgcttccgcc ttgccgactt
     8941 caaaatgctg gcggcgtgcg gcggcttcag ctacggcgac gtactcggcg cgggcgaagg
     9001 ctgggcgaaa tccatcctgt tccaccctgc cttgcgcgac caatttgccg ccttctttgc
     9061 cgatccggac acgctgacat tgggcgtgtg caacggctgc cagatggtca gcaacctcgc
     9121 cgagattatc ccgggtgcag aggcatggcc taaattcaaa cgcaacctga gcgaacagtt
     9181 tgaagcacgc ctgagcatgg ttcacgttcc gaaatcagcg tcgctgattc tgaacgaaat
     9241 gcaaggctcc agcctgcctg tcgtggtcag ccacggcgaa ggccgcgccg acttcgcgct
     9301 tcacggcggc aatatttccg ccgatttggg catcgcgctg caatatgtgg acggtcaaaa
     9361 ccaggttacc caaacctatc cgctcaaccc caacggctcg ccgcaaggca tcgccggcat
     9421 taccaacgcc gacggccgcg tgaccatcat gatgccccac cccgaacgcg tgtaccgcgc
     9481 cgcgcaaatg agctggaaac cggaaggctg gacggaactg tccggctggt accgcctctt
     9541 tgccggcgca cgtaaagcct tgggctaacc gccctactca aaccaatgcc gtctgaagaa
     9601 tatttcagac ggcgttccgg cataccatcc tttaaacggt atccgtccac cgaggaacac
     9661 tcatgaaaat cacccccgtc aaagccctaa ccgacaacta catctggatg atacagcacg
     9721 gcaaccatgc cgtctgcgtc gacccttccg aaccctcgcc cgtcttagaa ttcctcgtcc
     9781 gcaaccgcct catgcttgcc caaacatggg taactcaccc ccaccccgac cacgagggcg
     9841 gtgcggcggc actctggcgc ggctacatgg aatcgcccgt ttacggcgaa tccgacatcg
     9901 aagcagcaac ccacaccgta accgccggca cccaattcac cttcggcgac ggacaggtta
     9961 ccgtttgggc aacacccggc cacacagacc gccacaccag ctaccttctc gaaacttcag
    10021 acggcataca cgtcttttgc ggcgacaccc ttttttccgc cggctgcgga cgcgtgttta
    10081 ccggcacaat cgaacagctt tacgacagct tccaacgctt caaccgcctg cctgaaaaca
    10141 ccctgttcta cccggcacac gaatacaccg ccgccaacct gcgtttcgcc gcccatatcg
    10201 agccggacaa cgccgacatt cagacggcac tgaaagcggc ggcgcatacg cctaccctgc
    10261 ccgttaccct cgcgcacgaa cgccgcgtca atccgttttt gcgcgtcgac ctgccgcacg
    10321 tcagagaccg cgccgaggca ttgagcggga aaacgttaaa cagcagcctc gatacctttg
    10381 tcgcgctgcg tgaacttaaa aaccaatacc ggacgaaata aaacaatggg aaaacgcagc
    10441 cattcctagg atttttatta aaatcttaaa taaaatcata caatcatcgc caatagacga
    10501 aaggacaccg ttgccttata atcaaacaaa aacaaaatat ataatatagt ggattgaatt
    10561 taaatcagga caaggcgacg aagccgcaga tagtacggca aggcgaggca acgctgtact
    10621 ggtttttgtt aatccactat attgttaatc cactatataa atccagcaca aaacgggatc
    10681 ggtgattctt gtccgcaaga atcgttgatt ttctctatta cacggataat catcatgcgc
    10741 ttcacacaca ccaccccatt ttgttccgta ttgtccaccc tcggtctttt tgccgtttcc
    10801 cctgcttact catccattgt ccgcaacgat gtcgattacc aatattttcg cgactttgcc
    10861 gaaaataaag gcgcgttcgc cgtaggtgca agcaatattt ccatccaaga caagcaaggc
    10921 aaaatattag gcagggttct caacggcatc cccatgcccg acttccgagt cagcaaccgg
    10981 caaaccgcca tcgccaccct gattcacccc caatacgtca acagtgtcaa acacaacgtc
    11041 ggctacggtt ccatacaatt cggcaacgac acccaaaatc cagaagaaca agcctatacc
    11101 taccgcctcg tatcacgcaa cccgcacccg gactacgact accaccttcc ccgcctcaac
    11161 aaactggtta ccgaaatctc acctgccgca ctcagcagcg tacccttgct tggaaacggc
    11221 cagccaaagg ccaatgccta cctcgatacc gaccgcttcc catactttgt acgactcggc
    11281 tcaggcacgc aacaagtccg caaagcagac ggcacgcgta cacgaaccgc cccggcatac
    11341 caatacctga ccggcggcac gccgctgaaa gtattggggt tccaaaacca cggcttactc
    11401 gtcggcggca gcctgaccga ccaacccctt aacacctacg caatcgccgg agacagcggt
    11461 tcccccctgt ttgccttcga caagcatgaa aaccgctggg tgcttgcggg cgtactcagc
    11521 acctacgccg gcttcgataa cttcttcaac aaatacatcg tcacgcaacc cgaattcatc
    11581 cgttccacca tccgccaata cgaaacccgg ctggatgtcg ggctgaccac caacgaactc
    11641 atatggcgcg acaacggtaa tggcgacagc accctgcaag ggctcaacga acgcatcacc
    11701 ctgcccattg caaacccttc gcttgcccca caaaacgaca gcaggcacat gccgtctgaa
    11761 gatgccggca aaacgctcat cctatccagc aggttcgaca acaaaacact gatgctggca
    11821 gacaatatca accaaggcgc aggcgcattg cagttcgaca gcaacttcac cgtcgtcggt
    11881 aaaaaccaca catggcaagg tgcaggcgtt atcgtagccg acggcaaacg cgtcttctgg
    11941 caagtcagca accccaaagg cgaccggctc tccaaactgg gcgcaggcac gcttatcgcc
    12001 aacggacaag gcatcaacca gggcgacatc agcatcgggg aaggcactgt cgtactcgcc
    12061 caaaaagctg cttcagacgg cagcaaacaa gcattcaacc aagtcggcat caccagcggc
    12121 aggggcacgg ccgtcctcgc cgacagccag caaatcaaac ccgaaaacct ctatttcggc
    12181 ttcaggggcg gacggctcga cctcaacggc aacaaccttg cctttaccca tatccgccat
    12241 gcggacggcg gcgcgcaaat cgtcaatcac aaccctgacc aagccgcgac actgacgctg
    12301 accggcaacc ccgtcctcag tcccgagcat gtcgagtggg tgcaatgggg caaccgtccg
    12361 caaggcaacg cggcggttta cgaatacatc aacccgcacc gcaaccgtcg gaccgactac
    12421 ttcatactca aacccggcgg caacccgcgc gaatttttcc cgttaaatat ggaaaactca
    12481 acaagctggc aatttatcgg caacaacagg caacaggccg ccaaacaagt cgcccaagcc
    12541 gaaaatgccc gccccgacct aattaccttc ggcggatact tgggtgagaa cgcgcaaacg
    12601 ggcaaagccg caccgagtta cagcaaaacc aatatcgaat gggtgaatgg tccaaacacc
    12661 ccacacaagc cctcgcccta tacgaatacg ccaacccgca caggggcagg cgtaccgacg
    12721 attaccgcct caaagaaggc ggcaaccccg gcgcctactt cccatggaat gcctacagca
    12781 cccaagactg ggaatttctc ggacatgacc ggcgaagcag ccatagaaaa aacccgccat
    12841 atcgcaaatg ccgccgtata cggccggccc gaataccgtt acaacggcgc actcaacctg
    12901 cactatcgtc ccaaacgcac cgacagcacg ctgttgctca acggcggcat gaaccttaac
    12961 ggggaagtct tgattgaggg cggcaatatg attgtgtcag gcaggcccgt accccatgcc
    13021 tacgaccacc aggccaaacg cgaacccgtt cttgaaaacg aatggaccga cggcagcttc
    13081 aaggctgcac ggttcaccct gcgaaaccat gcccgactga cggcagggcg caataccgcg
    13141 catctggacg gcgacataac cgcatacgat ctgtccggca tcgacctcgg ctttatccaa
    13201 ggcaaaacac cggaatgcta ccgctcctac catagcggca gcacccactg cacacccaac
    13261 gccgttttaa aagccgaaaa ctatcgtgca ctacctgcaa cgcaagtacg cggcgacatt
    13321 acccttaacg accgttcaga gctccgcctg ggcaaagcac acctgtacgg cagcatccgt
    13381 gccggcaaag acaccgcagt ccgcatggaa gcagacagca actggacact ttcccagtcc
    13441 agccacaccg gcgcactgac gcttgacggc gcacaaatta ccctgaaccc cgatttcgcc
    13501 aataatacac acaacaaccg cttcaacaca ctgaccgtca acggcacact tgacgggttc
    13561 ggcacattcc gattcctgac cggcatcgtc cgaaaacaaa atgccccccc cctcaaactg
    13621 gaaggggaca gccgcggcgc attccaaatc cacgtcaaaa acaccggaca agaacctcaa
    13681 acaaccgaat cgcttgcact tgtgagcctc aatccgaaac acagccacca agcccgattc
    13741 accctccaaa acggctatgc cgatttgggt gcctaccgct acatcctccg caaaaacaac
    13801 aacggataca gcctgtacaa cccgctcaaa gaggccgaac ttcaaattga agccacgcgt
    13861 gcggaacatg agcgcaacca acaggcatac aaccaattac aggcaaccga catcagcaga
    13921 caggttcaac atgactctga cgcgaccagg caggcactac aggcctggca gaacagtcaa
    13981 accgaacttg cccgcatcga cagccaagtc caatatctgt ccgcccaatt gaaacagaca
    14041 gacccgctga ccggcattct gacgcgtgcc caaaacctgt gtgccgcaca aggatacagt
    14101 gtcgatatct gccgtcaggt tgccaaagcc gccgacacga acgacctgac actcttcgaa
    14161 accgaactgg atacgtatat agaacgtgta gaaatggccg aatccgaact tgacaaagca
    14221 cggcaaggcg gcgatgcgca agccgtcgaa acagcccggc acgcctacct gaacgcactc
    14281 aaccgtctgt cccgacaaat ccacagtttg aaaaccggcg ttgccggcat ccgtatgccg
    14341 aacctggccg aactgatcag ccggtcggcc aacaccgccg tttccgaaca ggccgcctac
    14401 aataccggcc ggcaacaggc gggacgccgc atcgaccgcc accttaccga tccgcagcag
    14461 caaaacatct ggctggaaac cggtacgcaa caaaccgact accatagcgg cacacaccgt
    14521 ccctaccaac aaactaccaa ctatgcacat atcagcatcc aaaccggcat caccgaccgt
    14581 ctcagtgtcg gtacaatttt aaccgatgag cgcacaaaca accgttttga tgaaggcgta
    14641 tccgcccgaa accgcagcaa cggcgcacat ctgttcgtca aaggggaaaa cggcgcactc
    14701 tttgccgcgg cagatttagg ctacagcaac agccgtaccc gatttaccga ttatgacggg
    14761 gctgccgtcc gccgccacgc atgggatgca ggcatcaaca ccggcatcaa aatcgatacc
    14821 ggcatcaacc tcagacccta tgccggcatc cgtataaacc gcagcaacgg caaccggtac
    14881 gtactcgacg gcgcagagat aaacagcccg gcgcaaatcc aaaccacatg gcatgccggc
    14941 atccgtctcg ataaaaccgt cgaactgggt caagccaagc tgacccccgc cttcagcagc
    15001 gattactacg atacccgcca aaacagcggt tccgccctca gcgtcaacga ccgtacctta
    15061 ctgcagcaag ccgcccacgg cacactgcat accctgcaaa tcgacgccgg atacaaaggc
    15121 tggaacgcca aacttcatgc cgcttacggc aaagacagca acaccgcccg ccacaaacaa
    15181 gcaggaatca aaataggcta caactggtaa caagccgata aaaatgccgt ctggaacccg
    15241 cgtttcagac ggcatttgcg ttaaaaatag taaaccgttc caaaagggag tagaatagtg
    15301 ccgtttccaa ccctgcgcct gtaccgtcag gcttttatta tggaccttcc cagttcgttt
    15361 ttactgaacg ccccttccga ttccaaacga caataaccat cccgccggaa tgcctccccg
    15421 cacacggcgg gcggagcatt tatgagcatc gaaccaaccc ctccgaacct tgaaaacgac
    15481 ggtatcgaaa acgatgtaga acgcgtttcc gccgatttcg accgtgtcca ctccctctgc
    15541 gaaattctcg aacctgcttt tgaacaaatc gaaaacggca caccgcttga agacgcgccg
    15601 ctgcgcgaca agctgaccga gctgaccgtt ctcttgagcg agctgcaccc tgccgacgtg
    15661 gcggcggtat tggaatcgct accgccgcgc gaacgcaata tcgtctggat tctggtcaaa
    15721 ccggaagacg acggcgaagt attgctggaa gtatccgacg cggtgcgcga aacgctgatc
    15781 gagtcgatgg acaaagacga attgttggca gcggtcgatg atttggacgc ggacgaattg
    15841 gcggaactgg cagacgattt gccgcaccaa gtggtttacg aagcgctaca aacccgcgat
    15901 gaggaagaac gcgcccaagt caaagcggca atgtcctacg aagacaacca agtcggtgcg
    15961 attatggact tcgagttggt cagcatccgc gccgatgtcg cctgtgaagt ggtgctgcgc
    16021 tatctgcgcc gcttcgacag cctgcccgac cataccgaca agatttttgt ggtcgatgaa
    16081 aacgacgtac tgcaaggcgt gctgcccatc cgcaaacttt tggtcgccga tcccgaagat
    16141 ttggtggaaa acgtgatggc gaaagatgtc gtgcgtttcc gcgccgaaga cgacgtggaa
    16201 gaagcggcgc aggcgtttga acgctacgac ttggtaactg cgccggttgt cgatgaaaac
    16261 aaaaagctca tcggtaggat taccgttgac gagatggtgg acgtcatccg tgaagaatcc
    16321 gaagcggata tgttgaacat ggcaggtttg caggaagagg aagacctgtt cgcccccgtc
    16381 ttggattccg tcaaaaaccg ctggatgtgg ctcgccgtca acctctgcac cgccttcctc
    16441 gccagccgcg tcatcggcgc gtttgaaggc agtatcgaaa aaatcgttgc actcgccgcg
    16501 ctgatgccca tcgtcgccgg tatcggcggc aactcgggca accagacgat taccatgatt
    16561 gtccgcgcca tggcgatggg gcagttgacg ggaatgcagg cggggcgctt gttgaaaaaa
    16621 gaagtcggcg ttgccctagt caacggcatt atctggggaa ccgtcatggg cgcggtttca
    16681 tggctgctct acggcagcct cggcatcggg ctggttatga ttgccgcgat gacgctcaac
    16741 ctcctgctgg cggcaaccgt cggcgtatta attcccgtgg taatggaaaa gttcggacgc
    16801 gatcccgcac tgggcagctc ggtgctgatt accgccgtta ccgactccgg cggcttcctg
    16861 attttcttgg ggctcgccac cctattcctg ctttaaatgc cgtctgaacc cgcgcaaaaa
    16921 tgccgtctga agcggaagct gcttcagacg gcatttgact atttatcctt gttgcacaag
    16981 attattggac ggtatgccgg ggcagccctt tggcaacgcc gaccacatcc tccccgaaca
    17041 gcgcgttgac atcggtttcg tcaaacacat atttgctgtg gcagaaatcg caatcgactt
    17101 cgatgctgcc ttgttccgcc accacgccgc cgacttcttc cccgcccagc atcaacagca
    17161 tatcgctgac tttgccgcgc gaacaggtgc atgaaaattc aaacgtttcc ggctcgaaca
    17221 cgcgcggcgg cgtttcgtgg aacaggcggt ataaaacgtg ttgcgcatcc aattctgcca
    17281 gctcctccgc cgtcagcgtg cgcgccagcg tactgacgtg ttcccatgcc tcttcatcca
    17341 acacctcttc aggcagacgc tgcaccagca gaccgcccgc cgcttcatcg cttgcagaca
    17401 ggacgatgtg cgtatcaagc tgttcggaac gtttcatata gttcatcaac atttgcgcga
    17461 taccgtcgcc ttccaaaggc actacgccct gccagggttc gccgtctttg ggctgcagcg
    17521 tcaatacgaa cacgccgttg ccgcccaaaa ggtcgccgag gctttcgtca tcggctattt
    17581 ctgccgcctc gtcccaccgc gcggtcgcac ggacggtacg gtcggaagtc gcttccgcaa
    17641 ccagcatttt cagccgcccc tgcccctgaa cctgcacaat cagcgtgcct tcgtttttga
    17701 ggttgcccga cagcagaaca ccggcagcca acagctcgcc caaggcgcgg cggatggcgg
    17761 cgggatagtt tttctgtttt acaatgtgct gccacacgtt ttccagacgg acgtgcagcc
    17821 cgcgcacggg catatcgtcg aagataaagc gggtacgcac atcggcgcgg ttgatggcgg
    17881 tttgattcat gattttctct gactgattgt tcggatggcg gctatatggt tgcggtcggc
    17941 gcgaaaacaa gacggacggc ggatgcgctt cccaaattat caataaatta tataaaaatc
    18001 aacatattaa ctcaatctaa caagccgttt tttgccaaac agccgttttt ttatatacaa
    18061 tcaacaagat attttcgact gatacagcat aacatcgcac ggcggcacga tgcctcctgc
    18121 gcggaaacac cgatatggat tcttttttca aaccggcagt ttgggcggtt ttgtggctga
    18181 tgtttgccgt ccgccccgcc cttgccgacg agttgaccaa cctgctcagc agccgcgagc
    18241 agattctcag acagtttgcc gaagacgaac agcccgtttt acccatcaac cgagcccccg
    18301 cccggcgggc gggcaatgcc gacgaactca tcggcagcgc gatggggctt aacgaacagc
    18361 ccgttttacc cgtcaaccga gtccccgccc ggcgggcggg caatgccgac gaactcatcg
    18421 gcaacgcgat ggggcttaac gaacagcccg ttttacccgt caaccgagcc cccgcccggc
    18481 gggcgggcaa tgccgacgaa ctcatcggca acgcgatggg acttttgggt attgcctacc
    18541 gctacggcgg cacatcggtt tctaccggtt ttgactgcag cggcttcatg cagcacatct
    18601 tcaaacgcgc catgggcatc aacctgccgc gcacgtcggc agaacaggca cggatgggta
    18661 cgccggttgc ccgaagcgaa ttgcagcccg gagatatggt gtttttccgc acgctcggcg
    18721 gcagccgcat ttcccatgtc ggactttata tcggcaacaa ccgcttcatc cacgcgccgc
    18781 gcacggggaa aaatatcgaa atcaccagcc tgagccacaa atattggagc ggcaaatacg
    18841 cgttcgcccg ccgggtcaag aaaaacgacc cgtcccgctt tctgaactga tttcccaagg
    18901 aatacgcaat gagtatgccc gaaataccca aatggtacga cgatgacgga cagatcgtgt
    18961 cctgtaccga aaagatcaaa gtgatgtccg aaaatatggc cgagctgtat cagacggcac
    19021 aagacgcgtt tgaagacgcg ctgctgatgg gttgcggcga acgtcagttg cgcgattacc
    19081 tgctcgcgct gattgaaggt ttggaaaatc cctaccgcaa agtctgaaca cgccccggtt
    19141 gctgcggcac ggtttatccg tgccgttttt gcgtttgtgc gcggcttcgg cttttcagac
    19201 ggcatatttg acgttatgat taaacagtta acaagattta tcacaacgcc gtcaaaagac
    19261 agacacacaa catgaacatc atccgcgcgc tcctcatcat cctcggctgc ctcgccgccg
    19321 gcgaaaccgc cgttttccta gcaggcatca aactgcccgg cagcatcgtc ggcatgggcg
    19381 tgctgtttgc gcttttgcag gcgggttggg tcaaaacgtc ttggctgcaa cagcttaccg
    19441 acgcgctgat ggcgaatctg acgctgtttc tcgtgccgcc ctgcgtggcg gtcatcagct
    19501 atttggattt gattgccgac gattggtttt cgatactggt ttccgcctcc gccagcactt
    19561 tgtgcgtact gctggttaca ggcaaggttc accgctggat acggagcatt atctgatgaa
    19621 cgaaatcctc aggcagccca gcgttctgct tttcctcacg cttgccgtgt acgcgcttgc
    19681 gattatcgtg cgcacgcgca cgggcaatat cttctgcaac cccgtactcg tcagcactat
    19741 cgtgctgatt gcctacctga aaatcctcga catcgattat gcggtgtacc acaacgccgc
    19801 gcagtttatc gatttctggc tcaagcccgc cgtcgtcgtg cttgccgtgc cgctctacca
    19861 aaaccgccgt aaaatcttca accaatggct gcccgtcatc gtttcacagc ttgcgggcag
    19921 cgttacgggc attgttacgg ggatgtattt tgccaaatgg ctgggcgcgg aacgcgaagt
    19981 cgtcctctcg ctcgcgtcca aatctgttac caaccccatc gccattgaaa ttacccgctc
    20041 catcggcggc attcccgcca ttaccgccgc caccgtcatc attgccggtc tggtcggaca
    20101 gattgccggt tacaaaatgc tgaagaacac agtcgtcatg ccctcgtccg tgggtatgtc
    20161 gctcggcacg gcttcgcacg cgatgggcat tgccgcctcg ctcgaacgca gccgccgcat
    20221 ggcggcatac gcggggctgg ggctgacgtt caacggcgta ctgaccgcgc tgattgcgcc
    20281 gctgcttatc cccgttttgg ggttctgaac ccgtttcaga cggcatttca gcccatgctg
    20341 tctgaacgcc gacacactcg caaggagaac cgttatggct gtcaacctga ctgaaaaaac
    20401 cgccgaacaa ctgcccgaca tcgacggcat tgccctctac accgcccaag caggcgtgaa
    20461 gaagcccggg cataccgacc tgacactgat tgccgtagcc gccggcagca ccgtcggtgc
    20521 agtcttcacg accaaccgtt tctgtgccgc gcccgtccac atcgccaaat cgcacctttt
    20581 cgacgaagac ggcgtgcgcg ccctcatcat caacacgggc aacgccaacg cgggtacggg
    20641 cgcacagggc agaatcgatg ctttggcagt gtgtgccgcc gccgcccggc aaatcggctg
    20701 caaaccgaac caagtgctgc ccttctccac cggcgtgatt ctcgaaccgc tgcccgcaga
    20761 caaaatcatc gccgcgctgc ccaaaatgca gcctgccttc tggaacgaag cggcacgcgc
    20821 catcatgacc accgacaccg tgcccaaagc cgcctcgcgc gaaggcaagg tcggcgacaa
    20881 acacaccgtc cgcgccacgg gcatcgccaa aggctcgggc atgattcatc ccaatatggc
    20941 gaccatgctc agtttcatcg ccaccgatgc caaagtttcc caacccgtcc tccaactgat
    21001 gacgcaggaa atcgccgacg aaaccttcaa caccatcacc gttgacggcg acaccagcac
    21061 caacgacagc ttcgtcatca tcgccaccgg caaaaacagc caaagcgaaa tcgacaacat
    21121 cgccgacccg cgttacgccc aactcaaaga attgttgtgc agcctcgcgc tcgaactcgc
    21181 ccaagccatc gtccgcgacg gcgaaggtgc gaccaagttc atcaccgtcc gcgtcgaaaa
    21241 cgccaaaacc cgcgacgaag cccgccaagc cgcctacgcc gtggcacgtt cgccgctggt
    21301 caaaaccgcc tttttcgcct ccgaccccaa cctcggcagg ctgctcgccg ccatcggtta
    21361 tgccggcgtt gccgacctcg ataccgacct cgtagaaatg tatctcgacg atattttggt
    21421 tgccgaacac ggtggacgcg ccgcaagcta caccgaagca caagggcagg cggtgatgtc
    21481 gaaggccgaa atcaccgtcc gcatcaagct gcatcgcgga caagccgccg ccaccgtcta
    21541 tacctgcgac ctgtcgcacg gatacgtttc catcaacgcc gactaccgtt cctgacccga
    21601 cacggcttca gacggcatac ataaaatgcc gtctgaaccg ccggacaaca taccatgacc
    21661 tccacattcc cccgccgcct cgcccgcaaa atccgccaaa cccgccgcct gtcgcgcaaa
    21721 agcatcgcct ttctgttcct tttggcaggt tcggcactcg tcgccctgac cgcgctgttt
    21781 tttgcccatc ttgccgattt tgcgctggaa ctgaacgcca aactggttca acaatacccg
    21841 tggttcgcgt gggtcgcgct tcctttgggt ttgccgctta ttgcgtggct cacacgcaaa
    21901 ttcgccccct tcaccgccgg cagcggcatc ccgcaggtca tcgcctcact gtcgctgccc
    21961 tacggcgcac agaaaacgcg gctgatccgc ctcgggcaga cgctgctgaa gattccgctg
    22021 acctttttgg gtatgctgtt cggcgcgtcc atcggacgcg aaggtccgtc cgtgcaggtc
    22081 ggcgcggcag tgatgggcgc gtggggcgcg tggtgcaaga aacacggctt ggcattcaaa
    22141 gggatgcagg aaaacgattt gatggcggcg ggcgcggcgg gcggtttggc agccgcgttc
    22201 aacgcgccgc tggcgggcgt gattttcgcc attgaggaac tcgggcgcgg catcatgttg
    22261 cgctgggaga ggcaaattct tttgggcgtg ctcgcctccg gtttcataca ggtcgccatt
    22321 cagggcaaca acccgtattt ttccggcttc aacggcggcg tattggaaca tatctttctg
    22381 tgggtcgcac tgtccggcct ggtttgcggc gcggcgggcg ggctgttcgg acgtttgctc
    22441 tatcgcggtg cggcggcatt tgcaccacgc aagatacgcg gcttcatccg caaccgtccg
    22501 ctgctgctgg cggcactgat ggggctgctg ctcgccctgc tcggcacgtt ctaccaaggc
    22561 aaaacctacg gcaccggcta ccacgaagcc gcccaagccc tgcacggcat ctatgaagcc
    22621 cccttcggac tcgccgccgc caaatggctc gccaccgtat tcagctattg ggcaggcgtt
    22681 ccgggcggca ttttcactcc ctcgctgacc ataggcgcgg ttttgggcga gcatatcgcc
    22741 gccatcgccg acatatcgca gggtgcaaac atcatcgtcc tcatctgcat ggcggcattt
    22801 ctggcgggcg cgacacaatc cccgattact tccgccgtcg tcgtcatgga aatgacgggc
    22861 ggacaaagcc tgctgttttg gatgctaatt gcctgcattt tcgcctcgca ggtttcgcgc
    22921 cagttttcgc cgcgtccgtt ctaccacgca tcgggaatgc gcttccgcca gcgcgtgctt
    22981 caggaaacca ccgcccaaac cggcaatgcg cccgcaagac cgcaaacagc aaacagcaaa
    23041 acgggaatgc cgtctgaaaa ttaaaacgcc cccgatcaaa cgccggcagc cgccttgatt
    23101 tgaataccgt tccgccgccg cttgaaattt cagcaacaat gccgtctgaa cgacagaatg
    23161 cggttttcag acggcatttc cacatcccga tattgcctaa acaaaaccga agcgtttgct
    23221 ataattctat tttttaccgc acacgcacca atcatgtttc ccgatttctc ccaaaccctc
    23281 tccaaagacc gccacttcct acgttccgcc ttcaaaaatc ccaacaaata cggcgggttg
    23341 tccaaagtcg aagaaaaata ccgaaaatcg cacgaaatct ttttgaaacg tttggcagcc
    23401 ttgcccaaac ccgaattcga caacaccctg cccgttcacg aaaaacttga cgaaatcaaa
    23461 aaaaccgttg ccgaacatca ggtaacgatt atttgcggcg aaaccggttc gggcaagacc
    23521 actcagttgc ccaagatttg tctggaactc gggcgcgggg cggcgggttt gatcgggcat
    23581 acccagccgc gccgtttggc ggcgcgttcc gtagcagagc ggattgccga agagctgaaa
    23641 tccgaaatcg gcagcgcggt cggctataaa gtgcgcttca ccgaccacac ctcgcgcgat
    23701 gcctgcgtca agctgatgac cgacggcatc ctgctggcgg aaacccagac cgaccgttat
    23761 ctcgccgcct acgacacgat tatcatcgac gaagcgcacg agcgcagcct gaacatcgac
    23821 ttccttttgg gctatttgaa acaactcctg ccgcgccgcc ccgatttgaa agtcatcatc
    23881 acctcggcaa cgatagacgc agaacgcttt tcccaacact tcaacggcgc gcccgtgctg
    23941 gaagtgagcg gacgcaccta tcccgtcgaa atcctctacc gaccgctgat cggcaaagac
    24001 gaagacgacg cggaaataga attgaccgac gcgattgtcg atgcggcgga cgaattggcg
    24061 cgatatggcg aaggcgatat tttggtattc ctgccgggcg aacgcgaaat ccgcgaagcc
    24121 gccgaagccc tgcgcaaatc cacgctacgc cgcaacgacg aaatcctgcc cctgttcgca
    24181 cgcctgtcgc acgccgagca gcacaaaatc tttcacccct ccggagcgaa acgccgcatc
    24241 gtgctggcaa ccaacgtcgc cgaaacctcg cttaccgtgc cgggcatcaa atacgtcatc
    24301 gacaccggcc tcgcgcgcgt caaacgctat tccgcacggg cgaaagtgga gcagcttcat
    24361 atcgaaaaaa tctcccaagc cgccgcccgc caacgctccg gccgctgcgg acgcgtctcc
    24421 gcaggcgtat gtatccgatt gttttcagaa gaaaatttca acaaccgccc cgaatttacc
    24481 gaccccgaaa tcgcccgcag caacctcgcc gccgtcatcc tgcgcatggc atcgctgaaa
    24541 ctcggcgacg tagcggcatt cccattttta gaaatgcccg attcgaggta tattaatgat
    24601 gggtttcagg tgttgctgga attaggggca gtggaggccg cctgaaaaca agtaggatat
    24661 acacttctac ctgacaaaga ccaacaaacg ataaaactac ggcacatcta gatgcccaac
    24721 ctacaacatt gcacacggta aaaacatgaa acacttactc atcgattttg aaaacgtcca
    24781 gccgcaaaac ttagacaaat tactaaccga aaatacccat atttggctat ttataggtgt
    24841 attacacaaa atgttaccta ttagtctggt gcaatcccta ctacgtttcg gcgaacgtgt
    24901 ccatcttgtc cagttacaaa aaacggggaa aaacgcattg gatttttacc tgtcctatta
    24961 cctcggacaa attaccgcca cagaccccaa tgcccaaatc ggcatactct cgcgtgatgg
    25021 aggatacgat gttctggtcg aacatatttt gaaaaaccac caggcgaagg gtatcgtgcg
    25081 cctagccaat atagatgaag tacaacatca gaaaattact accgaaccgc cgtcagcatt
    25141 gctggaaaac actcctcagc ctgaaaccac cctcaaacca cagcaaccat taacttccta
    25201 tttccaagca gccctaactg cactgcgccg ccccgacgct ttccgcccct gccgcctgca
    25261 taacctgcga caaaatctgc gtaagcatat tttgagtgat ttgtttaaag aaaaaaccga
    25321 tgaagaatgc gaaataacca ctgctaacgt tatcaataaa ctcaaagcac aaaacttcat
    25381 cagcattgat gaacaggaaa ccgtttccta ccatctcagt gataatgatt tgttacaaag
    25441 aatccaacgc catattttaa gccaacgtcc caaaacctac gctgattttc aagccgtcgt
    25501 gcaaaaccga gcagatgcac ttcacttaac agtcggtacc aacgacattc aatcctttgc
    25561 gcgacatttg cgcgaccaaa acctgatccg ccaaaacaat gggaaaattg aatatgcacc
    25621 gtttactgaa cctaaaccac agccaacgcc caagcagcct aaaaaaaccg catgggaacc
    25681 tgatgaaatt atttggaaaa aagtgattgc cgcgttatcg ttaaagaacc gtcctaataa
    25741 aaccaaaact ttacgcaata caatccaggc actcacaaaa tccaatgcac aagaaactga
    25801 caaactgcta caacatttac aagataccca agtcctacgt attgacggta ctaaaattgt
    25861 gtatatcaag taggatgggc atttatgcct gacctacaaa accaaaaaca acctaacacc
    25921 cttaatcaac tccacaaacc cttttcagac aacctcgttt tttgaaaaac aatctgtaaa
    25981 cagataactg ctgaagaata ccgttgccga gccccaaaac ctgcctgcaa cttttattgt
    26041 gaactgccca ttatgaaaaa atcccttttc gttctctttc tctattcatc tctacttacc
    26101 gccagcgaaa tcgcctatcg ctttgtattc ggaattgaaa ccttaccggc tgcaaaaatg
    26161 gcagaaacgt ttgcgctgac atttgtgatt gctgcgctgt atctgtttgc gcgttataaa
    26221 acttcgcggc tgctgattgc gctgtttttc gcgttcagca ttattgccaa caatgtacat
    26281 tatgcggttt atcaaagttg gatgacgggt attaactatt ggctgatgct gaaagaggtt
    26341 accgaagtcg gcagtgcggg cgcgtcgatg ttggataagt tgtggctgcc tgcgttgtgg
    26401 ggcgtggcgg aagtcgtgct gttttgtagc cttgccaagt tccgccataa gacgcatttt
    26461 tctgccgata tattgtttgc cttcctaatg ctgatgattt tcgtgcgttc gttcgacacg
    26521 aaacaagagc acggtatttc gcccaaaccg acatacagcc gcatcaaagc caattatttc
    26581 agcttcggtt attttgtcgg acgcgtgttg ccgtatcagt tgtttgattt aagcaagatt
    26641 cctgtgttca aacagcctgc tccaagcaga atcgggcaag gcagtattca aaatatcgtc
    26701 ctgattatgg gcgaaagcga aagcgcggcg catttgaaat tgtttggcta cgggcgcgaa
    26761 acttcgccgt ttttaacccg gctttcgcaa gccgatttta agccgattgt gaaacaaagt
    26821 tattccgcag gctttatgac ggcagtatcc ctgcccagtt tctttaacgt cataccgcat
    26881 gccaacggct tggaacaaat cagcggcggc gataccaata tgttccgcct cgccaaagag
    26941 cagggctatg aaacgtattt ttacagcgct caggctgaaa accaaatggc aattttgaac
    27001 ttaatcggta agaaatggat agaccatctg attcagccga cgcagcttgg ctacggcaac
    27061 ggcgacaata tgcccgatga gaagctgctg ccgctgttcg acaaaatcaa tttgcagcag
    27121 ggcaggcatt ttatcgtgtt gcaccaacgc ggttcgcacg ccccatacgg cgcattgttg
    27181 cagcctcaag ataaagtatt cggcgaagcc gatattgtgg ataagtacga caacaccatc
    27241 cacaaaaccg accaaatgat tcaaaccgta ttcgagcagc tgcaaaagca gcctgacggc
    27301 aactggctgt ttgcctatac ctccgatcat ggccagtatg ttcgccaaga tatctacaat
    27361 caaggcacgg tgcagcccga cagctatctc gtgccgctag tgttgtacag cccggataag
    27421 gccgtgcaac aggctgccaa ccaggctttt gcaccttgcg agattgcctt ccatcagcag
    27481 ctttcaacgt tcctgattca cacgttgggc tacgatatgc cggtttcagg ttgtcgcgaa
    27541 ggctcggtaa cgggcaacct gattacgggt gatgcaggca gcttgaacat tcgcgacggc
    27601 aaggcggaat atgtttatcc gcaatgagtg gcgtaaaaac caataaagac aaattaagat
    27661 gatgtcgggg aagatgcccg accgacaaga ctatgcaaaa tatgaaaaac caagtacgcg
    27721 gatcaggcat ggatgcacga tccaatccgg ccaatgtttc agacggcctg caaaacagtt
    27781 cgagccatat cggtaccaac acgcgctacc gcctgaccaa gctcggcgaa caaatggcgc
    27841 gcctgcccat cgacccgaaa atcgcgcgca ttttgctggc ggcgaagaaa cacgactgta
    27901 tggcggaaat attggtgatt gcgtccgcgc tgtcgattca agacccgcgc gagcggccgt
    27961 tggaagcgcg cgatgctgcc tccaaggcac acgaacgttt taccgacaag cagtccgatt
    28021 tccttgccta tctgaacatt tgggacagct tccagcgtga gcgcgacaaa ggtttgtcca
    28081 acaagcagct ggtgcagtgg tgccgccaat atttcttgtc gcacctgcgg atgcgcgaat
    28141 ggcgcgagct acaccaccag cttgcccaaa ctgcgattga aatgggtttg accaccaaag
    28201 aagccgcttt cagacgttct cccgaagtca ggcagctcac gtcgtctgaa aatgcaggcg
    28261 accaagacct atccgccaaa ctcaaacaaa aacaactgga taagaaacaa caccgcgccc
    28321 aaatccgcgc tgccaaagaa gcgggctacg aacaaatcca ccgcgccctg ctcaccggcc
    28381 tcatcgccaa cgtcggcatg aaatcacccg acggcaacgt ctacaccagc acgcgcggca
    28441 gccgcttcca ccttttcccc gcctccgccc tgttcaaggc gaagcccaaa tgggtgatgg
    28501 cggcagaact cgttgaaacg acaaaacttt atgcccgcga cgtagccgcc atccagcccg
    28561 aatggatcga gcaggaagcg ccgcacctcg tccgctacca ctatttcgag ccgcattggg
    28621 aacaaaaacg cggcgaagtc atcgccagcg aacgggtaac gctctacggc ctgaccgtcc
    28681 tgccgcgccg ccccgtgtcc tacggcagaa tcgcccccga agaagcgcgc gaaatcttta
    28741 tccgcggcgc attggtggca caggaatgcg atttgaaagc ggactttttt gtccacaaca
    28801 aaaagctgat taaagaaatt accgaactcg aacacaaatc gcgcaagcaa gacgtgctgg
    28861 tcgatgacga agccctgttt gcgttttata acgaacgact gcccgaaatg gcttggaaag
    28921 acgcgcaagg cagcgtttgg ggaagtgaag attccgtacg gattattgaa tctgacaaag
    28981 ccgagaggtc gtctgaaaat gagcgcaacg agtttcgtaa aaacaagcgt aatgggtctc
    29041 gccaaaatga aaatcacggc aacaccgtag gttgggttga aaacccaaca tcagccgcaa
    29101 ctgcaaaaac tgttgggttt gacaacccaa cctacgctac tcagcaaccc accccctccc
    29161 ccgagaggga gggtcgggga gagggcaaaa cagttgccgc acaaaccaac ttttccgcaa
    29221 ccgcagcaaa ccctctcccg caggagaggg aacagagtgc ctcagcttca acgttttcag
    29281 acgacctgcg tcctgcaaat ctgcagcaaa ccgccccctc ccccgtgggg gagggctggg
    29341 gagagagcaa aacagttgcc acccaaacca acttttccgc aacctcaaca ctttcagacg
    29401 accccaaacc caaaaagcag cctgcacctc aaaaaggccg tctgaaaccc ctacccctcg
    29461 ccgacatccg caccttccaa gcctggctca aaaccgccga gtgcgacaat ccgcgcctgc
    29521 tgttcctcag ccgcgacgat ctgatgcaac acgccgccgc acacattacc gaagaacagt
    29581 tccccaaatt ctggcaaacc gcagacggca aattcaaact ttcctaccgc ttcgagccgc
    29641 accatccgct agacggcgtg accatgaccg tgccgctgac cgtcctcaac cgcctgcacg
    29701 cgccatcgct cgaatggctg gtgcccggta tgttgcgcga aaaaatccag ctgctgatta
    29761 aagcactgcc gaagcaaatc cgccgcattt gtgtgcccgt acccgatttc atcaccaaat
    29821 tcctcgaaag caatcccgac cgccaagcga ccatcattcc ccagttggcg cactttatcg
    29881 ccaaaagcgc cagcgatatg cggattctcg agcaaatcga ccaagacgca tgggcggcgc
    29941 aagaattgcc cgaacactgc tatctgaatc tgcgcattat cgacgacggc ggacaagagc
    30001 ttgccggcgg ccgcaaactg cacgaattgc aacaacagct cggccaagcc gccgccgtca
    30061 ccttccgcga caacacccaa gaattcgagc gcgacaacgt taccacatgg gacatcggaa
    30121 ccctgcccga atccatcaaa ttcgcccgcg gcaaacaaca gctcaccggc tacctcggcc
    30181 tgcaaaaaga aaaagacggc cgcatcgccc tgcgcctgtt tgacaccacc gaagccgccg
    30241 aacaggcaca ccgtcaaggc gtcatcgaat tgatgaagct gcaattaaaa gagcaggtaa
    30301 aagacctgaa caaaggcatc caaggcttca cccaagccgc catgctgctc aaacacatca
    30361 acgccgacac cctgcgcgac gacctcaccc aagccgtctg cgaccgcgcc tttatcggcg
    30421 aagacgagct gccgcgcaac gaaaaagcct tcaaagaaca aatcaaacgc gcccgtagcc
    30481 gcctgcccgc cgtcaaagaa gccctcagcc gctacctgca ggaaaccgca gccgcctacg
    30541 ccgaactcaa cggcaaactc ggcaaacatc cattgaccca cctcctaaga ctacgcctgc
    30601 aaaccctgct tgcccccggc ttcgccaccc gcaccccgtg ggcacaatgg ccgcgcctcc
    30661 ccatctacct caaagccatg accctgcgcc tcgaaaaata cagcagcaac cccgcccgcg
    30721 acgcagcccg cgaagccgat atacaggaat tggaacaaat gtggcaggaa aaaaacgacg
    30781 gtttggtgaa acaaggacaa cccatttcag acaacctagc cgcgtttaaa tggatgattg
    30841 aagaattgag agtgtcactg ttcgcacagg aattgaagac accgtatcca gtgtcggtga
    30901 agaggttgtt aaaagagtgg gagaaaattt tttaactgaa taattctgcc gatcaaaatt
    30961 tcttgacaaa acaaatttta atgaatacta taagtagcat tatgagttta gaaaattttc
    31021 aattaataaa aacacaatta ggtgttccca gttatgaaaa tgatagagtt gcaatctatc
    31081 aaggagattg tatccagtta atggataaga tttcaaaagg atcattccaa atgacaatca
    31141 catctccacc atacaatatt ggtaaagaat atgagtctat tttagatttg gaacattatc
    31201 ttgagtggtg tgaaagatgg atgcaatcaa tacatcactt aactgaagat agcggatgtt
    31261 tttggttaaa tttaggatac ttacatgttg aaaaaaaggg actagcagtt cctattcctt
    31321 atcttttatg ggataaaact aactttttcc ttttacaaga ggtggtttgg aattatgcag
    31381 ctggagttgc atgccgtaat cgattttccc caagaaatga aaagttatta tggtatgtga
    31441 aaaatcctgc taaatataca ttcaatttag atgctgtacg cgatcccaac gttaaatatc
    31501 caaatcagaa aaaaaatggg aaattaaaat gtaatcctct aggaaaaaac ccaacggatg
    31561 tttggcaaat agcaaaagtc acttctggaa gaaataggtc atcgagagaa agaactgctc
    31621 atcctgccca atttccattg gaattaatag aaagaataat ccgagcaagt agcaatcaaa
    31681 atgatgtaat cttagatcca tttatcggct caggaacaac cgccttagcc ggactaaaaa
    31741 acaaccgttt tgtcatagga tttgaaatta acgatgagta tatagatatt ataaaagaac
    31801 gcttcaaaga gtatgatctt caaaatactt gatacttgta tataacggta tagaggatag
    31861 accctcctct atactgtatt tacaatattt atttttgata aagtattttt agtttacttc
    31921 tataagcctc tggagagagc ctcgcctgct ggcctgtcgc tgcagtttga gcaatccaat
    31981 ccgtatgatc taaccaacca aatctgataa ttttaatttc tggacgcaat tttaaatcag
    32041 gcataaattt ttcaaaattt atagttagat aataaccatt tttactctta ccagtaattc
    32101 catcttgtgg ttgtgcataa ctcctattac caaatatata ttttggatcc gatgaagttt
    32161 ttaattctat tgagtaatag tcgtctttaa tatatacaat atctttgtca tatttattct
    32221 tttctccttg ccaattttca aatctagcct caatattagc aggaattaaa gcatgtaata
    32281 acgcccctac aatctgagga gaaggaaaaa aatttttgcc aatttgcaaa ttaccaattc
    32341 tagacgagaa aatattttcc caactatcta aacaaagttg gacaatctct ttttctgaca
    32401 atggatgctg cttaatcagc tcttctgttt tggaaatcca taactcctca ttcaaagaag
    32461 catacggcga tatttttgat ctattcatat tatttaatcc cttctacaag ttcagaaata
    32521 ttaattgaaa aagacttagc taacagcgag atattcaaaa gacttatatt tctttctccg
    32581 cgttcaactg atccaatata agtacgatgt aatccaactt tttctgctaa ttcttcttgt
    32641 gaccaaccat taagtaaacg ataatgtttt acacgcttac caaactcaac taattcattt
    32701 tccataatct gtattatttt aataagtgaa tactttaagt ctacactcta taagtagcac
    32761 acttaacaaa ccatatccta ctgcgtgaat accatagcaa actgtagcct gcatcaaacc
    32821 gcgtgcatgc cttcgggcac acactcggtc tttctaaacc actccccctt tccacccaaa
    32881 ccaaaggtcg tctgaaaccc attttcagac gaccttttgc cactttgcaa agcaagtatt
    32941 cccaccaccc tctccccaaa tatcgcccaa accagtaaac ttcccatatt tcaacaactt
    33001 cttgaaagcc aaccatgtcc ggtatctacc taccccacct atttccgccc catatcgccg
    33061 aacgcggcct tttgtatttt cagcagggca aggttcgcga tgtccgaaaa acttccgccg
    33121 ggcgttatcg ggcctagcgc tgtttgctgc cggcggtctg aataatggtg gcgtcaacga
    33181 cggcagcgga tgctttctct acttttaggt ttttttcggt cagttggcgg ttaatcagtt
    33241 tgagcaattc ggacagggtg tcgtcttgcg ccagccagtt gcggtaacgg cataaggtgc
    33301 tgtaatcggg gatgctcagt tcgtcaaagc ggcaaaacag gttgaagtcg atgcgggtga
    33361 tgaggctgtg ctcgagttcg ggatcggaga ggctgtgcca ttgtccgagc agaacggctt
    33421 tgaacatgga caacagcggg taggcgggac gaccgcggtg gtctcggagg taacgggttt
    33481 tttgacggtt caggtattgt tcgatcggtt gccaatcaat cacctggtcc aacttcaata
    33541 gtgggaaacg gtcgatgtgt ttggcaatca tggcttgggc ggtttgctga aagaaggtgc
    33601 tcatgagaaa tctcctaaat gtcttggtgg gaatttaggg gattttgggg agttttgcaa
    33661 aggtctcagt ctttttaaac cacagccctt cccaactaaa ccaaaaggtc gtctgaaccc
    33721 tattttcaga cgaccttttg ccactttgta aaacaaatct tcccaccatc ctctccccaa
    33781 acatcgcccg aaccagtaaa cttctcatat ttcaacaact tcttggaagc aaaccatgcc
    33841 ccatatccac ccgctccacc tgttcccgcc ccatatcgcc gaacgcggac tgctctattt
    33901 tcagcagggc aaagttggtg ctgtccaaaa aacttccacc gggcgttatc gggcggaggt
    33961 atgcggtttg gaaaactatt gggtatggct gaagctggat agtgatttgt atattaaaga
    34021 cgaaagctgc gattgccctt atgtctaaag gtgcaaacat accttaaatt actatattac
    34081 ataggcaaaa tacaagccta taacgaattg gaaacaaaat gccgtctgaa agcattataa
    34141 aatctgttca ccttttcaga tgagtaatgt acacccctat acaatttttg ctactatacc
    34201 ccataaatcc acggctaaag atatccttat tatgtcctat gatttatcga aacgacttgt
    34261 aatcggctta gcatcaagtg ccctattcga cttatccgaa tcggataata tatttagaat
    34321 ggaaggggca gaaacctata ggcaatatca gagagaaaaa caaaaccatc ccctaaaaaa
    34381 ggcgttgtct ttccatttat taaaaaactt ctgtcaatca atgaaataaa cccaaacgac
    34441 ccaacaattg ggtttattct tttatccaga aacaatccag atacagatta cgagtcataa
    34501 ctataggctt aatattacac gattctcatt ccatcaaggc ggaaaaccgc acaaatactg
    34561 aaacactatc gatcgatttg taaacaagcc tacttaagta acttgcagtc cttatcattt
    34621 cctttaaaat aatccagccc gtcactacac gaactggcgg acttcttgca aataaaggtt
    34681 actagatttt cattcatctt aataataaaa ggatttttat ctttatctat ggctaccgcc
    34741 ttcaacatga atttactgtc taaggccccg cgcgcgattc cattcaaacg gatacaaaag
    34801 ccttctgcct ctttaatcgg caaacttggc catttggtag atgtttgttt aaatctccca
    34861 ttctgcagat aaaacttttc cataaaatgt gcattttcta acaaggctgc ccgcactgca
    34921 tttatctttg ctttctcaac ataattgcga tagctcggat aaacaattaa agcaagtaca
    34981 gacaatatca agaccactga tattaattca accagcgtaa acccccgatt atcagtcatt
    35041 actttacttc caataagaac agattattca acatatttct ttgaacagac ttactatccc
    35101 attcagcagt atgcatattt cccactctat tttttatccg cgggcagc
//
LOCUS       DAXHPG010000003        34837 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXHPG010000003 DAXHPG010000000
VERSION     DAXHPG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34837
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.003"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            205..699
                     /locus_tag="ACFM6F_000087"
     CDS             205..699
                     /locus_tag="ACFM6F_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163589.1"
                     /translation="MENRFAKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCDISHFLHFADWA
                     EADIPNQSRLYAEYWWEMEIFKMSVLEDWERSGKPLDWEKWQRQYRSEAFELVKKLNR
                     SKQE"
     gene            819..1952
                     /locus_tag="ACFM6F_000088"
     CDS             819..1952
                     /locus_tag="ACFM6F_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163590.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESIRTEENLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYHIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(2039..2314)
                     /locus_tag="ACFM6F_000089"
     CDS             complement(2039..2314)
                     /locus_tag="ACFM6F_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163591.1"
                     /translation="MNEGEVVLTPEQIQTLYGYASRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKIRLMCISVFWIVVFNL"
     gene            complement(2331..3104)
                     /locus_tag="ACFM6F_000090"
     CDS             complement(2331..3104)
                     /locus_tag="ACFM6F_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6163592.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPE
                     YEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNNRINQTEEDNYSTGVGSCINGFP
                     VRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(3503..3778)
                     /locus_tag="ACFM6F_000091"
     CDS             complement(3503..3778)
                     /locus_tag="ACFM6F_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6163593.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(3775..5058)
                     /locus_tag="ACFM6F_000092"
     CDS             complement(3775..5058)
                     /locus_tag="ACFM6F_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6163594.1"
                     /translation="MTVSKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRVPILGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPSVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWIFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(5209..6627)
                     /gene="glnA"
                     /locus_tag="ACFM6F_000093"
     CDS             complement(5209..6627)
                     /gene="glnA"
                     /locus_tag="ACFM6F_000093"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC6163595.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWSSGLHLDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEELGIKVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            6931..7740
                     /gene="aroE"
                     /locus_tag="ACFM6F_000094"
     CDS             6931..7740
                     /gene="aroE"
                     /locus_tag="ACFM6F_000094"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC6163596.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHRQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLILLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVNPEIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKQTADGLGMLVGQAAASYALWRGFTPNIRPVIEYMKAM"
     gene            7742..8443
                     /gene="mtgA"
                     /locus_tag="ACFM6F_000095"
     CDS             7742..8443
                     /gene="mtgA"
                     /locus_tag="ACFM6F_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC6163597.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGIFGAEAASRYFYQIPAAKLSKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(8768..8843)
                     /locus_tag="ACFM6F_000096"
     tRNA            complement(8768..8843)
                     /locus_tag="ACFM6F_000096"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8808..8810),aa:Phe,seq:gaa)
     gene            complement(9034..9768)
                     /gene="lptB"
                     /locus_tag="ACFM6F_000097"
     CDS             complement(9034..9768)
                     /gene="lptB"
                     /locus_tag="ACFM6F_000097"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC6163598.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(9842..10372)
                     /gene="lptA"
                     /locus_tag="ACFM6F_000098"
     CDS             complement(9842..10372)
                     /gene="lptA"
                     /locus_tag="ACFM6F_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC6163599.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(10353..10934)
                     /gene="lptC"
                     /locus_tag="ACFM6F_000099"
     CDS             complement(10353..10934)
                     /gene="lptC"
                     /locus_tag="ACFM6F_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC6163600.1"
                     /translation="MKIRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(10931..11467)
                     /locus_tag="ACFM6F_000100"
     CDS             complement(10931..11467)
                     /locus_tag="ACFM6F_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC6163601.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(11532..12506)
                     /locus_tag="ACFM6F_000101"
     CDS             complement(11532..12506)
                     /locus_tag="ACFM6F_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC6163602.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTIAAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            12602..13657
                     /gene="tal"
                     /locus_tag="ACFM6F_000102"
     CDS             12602..13657
                     /gene="tal"
                     /locus_tag="ACFM6F_000102"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC6163603.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALEILVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDRLK
                     GKIAIALAKAAYQDWAQYFGSSEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            13672..13806
                     /locus_tag="ACFM6F_000103"
     CDS             13672..13806
                     /locus_tag="ACFM6F_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163604.1"
                     /translation="MPSERLSDGIALLTGHSAAYQGFALKILINKKLMHDITHQHKRK
                     "
     gene            13811..14101
                     /locus_tag="ACFM6F_000104"
     CDS             13811..14101
                     /locus_tag="ACFM6F_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163605.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELTKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIIALINSLK"
     gene            14118..15005
                     /gene="gluQRS"
                     /locus_tag="ACFM6F_000105"
     CDS             14118..15005
                     /gene="gluQRS"
                     /locus_tag="ACFM6F_000105"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC6163606.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQTGARRGADGFVYNGRCRNPQQRPALQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMGKVPKHAITAP"
     gene            complement(15075..16085)
                     /gene="dusA"
                     /locus_tag="ACFM6F_000106"
     CDS             complement(15075..16085)
                     /gene="dusA"
                     /locus_tag="ACFM6F_000106"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC6163607.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPS
                     PRVQKGAFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EEIAQHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKNARTWRRMLSDATLLKDNDGGLILEAWKEVERANM
                     RE"
     gene            complement(16116..16607)
                     /gene="dtd"
                     /locus_tag="ACFM6F_000107"
     CDS             complement(16116..16607)
                     /gene="dtd"
                     /locus_tag="ACFM6F_000107"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC6163608.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADATSGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(16681..17439)
                     /locus_tag="ACFM6F_000108"
     CDS             complement(16681..17439)
                     /locus_tag="ACFM6F_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6163609.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(17510..18376)
                     /locus_tag="ACFM6F_000109"
     CDS             complement(17510..18376)
                     /locus_tag="ACFM6F_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6163610.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(18425..18703)
                     /locus_tag="ACFM6F_000110"
     CDS             complement(18425..18703)
                     /locus_tag="ACFM6F_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6163611.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(18703..18993)
                     /locus_tag="ACFM6F_000111"
     CDS             complement(18703..18993)
                     /locus_tag="ACFM6F_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC6163612.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(18996..19526)
                     /locus_tag="ACFM6F_000112"
     CDS             complement(18996..19526)
                     /locus_tag="ACFM6F_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC6163613.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            19834..22287
                     /gene="tspA"
                     /locus_tag="ACFM6F_000113"
     CDS             19834..22287
                     /gene="tspA"
                     /locus_tag="ACFM6F_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
                     /protein_id="HFC6163614.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETV
                     AETPDFNATADDLSALLQPSKVPAVEENAAETVADDLSALLQPAEAPAVEENVTETVA
                     ETPDFNATADDLSALLQPSEVPAVEENAAEITLETPDSNTSEADALPDFLKDGEEETV
                     DWSIYLSEENIPNNADTSFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLE
                     EAEGDVLKRAQALAQELGI"
     gene            complement(22459..22875)
                     /gene="gloA"
                     /locus_tag="ACFM6F_000114"
     CDS             complement(22459..22875)
                     /gene="gloA"
                     /locus_tag="ACFM6F_000114"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC6163615.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLCRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAIEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(22922..24091)
                     /gene="lapB"
                     /locus_tag="ACFM6F_000115"
     CDS             complement(22922..24091)
                     /gene="lapB"
                     /locus_tag="ACFM6F_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC6163616.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(24102..24416)
                     /locus_tag="ACFM6F_000116"
     CDS             complement(24102..24416)
                     /locus_tag="ACFM6F_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC6163617.1"
                     /translation="MKLIYTFIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTK
                     QP"
     gene            24726..25724
                     /gene="ilvE"
                     /locus_tag="ACFM6F_000117"
     CDS             24726..25724
                     /gene="ilvE"
                     /locus_tag="ACFM6F_000117"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC6163618.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYSSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(25908..26693)
                     /gene="fabI"
                     /locus_tag="ACFM6F_000118"
     CDS             complement(25908..26693)
                     /gene="fabI"
                     /locus_tag="ACFM6F_000118"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC6163619.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNAAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(26848..27669)
                     /gene="dapD"
                     /locus_tag="ACFM6F_000119"
     CDS             complement(26848..27669)
                     /gene="dapD"
                     /locus_tag="ACFM6F_000119"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC6163620.1"
                     /translation="MSLQNIIETAFENRADITPSTVAPEVKEAVLETIRQLDSGKLRV
                     AECLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(27679..27909)
                     /locus_tag="ACFM6F_000120"
     CDS             complement(27679..27909)
                     /locus_tag="ACFM6F_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163621.1"
                     /translation="MPSERRRRGIKVPPPFCIDSGAEKAIPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILLENTGKRPTKHLT"
     gene            complement(27879..29522)
                     /gene="pgi"
                     /locus_tag="ACFM6F_000121"
     CDS             complement(27879..29522)
                     /gene="pgi"
                     /locus_tag="ACFM6F_000121"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6163622.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYVDGRDVLPEIRRELNRALEFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSSVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGVIWDINPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGVGAA"
     gene            30032..31264
                     /gene="pilG"
                     /locus_tag="ACFM6F_000122"
     CDS             30032..31264
                     /gene="pilG"
                     /locus_tag="ACFM6F_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC6163623.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            31338..32198
                     /locus_tag="ACFM6F_000123"
     CDS             31338..32198
                     /locus_tag="ACFM6F_000123"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC6163624.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKKHLNLPLTEKESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            32200..32832
                     /gene="coaE"
                     /locus_tag="ACFM6F_000124"
     CDS             32200..32832
                     /gene="coaE"
                     /locus_tag="ACFM6F_000124"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC6163625.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFIILIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHNG"
     gene            32825..33034
                     /locus_tag="ACFM6F_000125"
     CDS             32825..33034
                     /locus_tag="ACFM6F_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC6163626.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(33119..34795)
                     /gene="pilB"
                     /locus_tag="ACFM6F_000126"
     CDS             complement(33119..34795)
                     /gene="pilB"
                     /locus_tag="ACFM6F_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC6163627.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         8335 a         9528 c         8879 g         8095 t
ORIGIN      
        1 gttatcagct cgacagaacc tgtattttat tcaaaataac aaattatcaa acaaagctct
       61 gattaaaaac ccaacaatca aaatgccgtc tgaagcggta ttcggctttc agacggcatt
      121 ttctccgatt agcacaggat gccggggctt gccggcttga ttttgccgaa attgttggca
      181 cgcaattggg aggggcggca acctatggaa aatagatttg caaaaatcgt taaggatatt
      241 tttagtttta cccaatttgt ttccgaagcg gaaaataaaa ttcctaacca atcctctcgt
      301 tattacgatt gctggtggga attggaaatc ttgaatgctt ccgcattgga agattgggaa
      361 atatcgggaa agcctgaaaa atgggagaaa tggacagaaa aatatcagac cgaagcgtta
      421 cagtcagtca agcatctatt tgccgaagaa gttcctgaaa ataatatagt ttgcgatatt
      481 tctcattttt tgcattttgc cgattgggca gaagccgaca ttcccaatca atcacgttta
      541 tatgccgaat attggtggga aatggaaatt ttcaaaatgt cggttttaga agattgggaa
      601 cgctcgggaa aaccgttaga ttgggaaaaa tggcaaaggc aatatcgatc tgaagcattt
      661 gaattagtta aaaaattgaa ccgaagcaaa caagagtaac aagccgcagc ctgcataggg
      721 gtttttaaag tacggcagca accgtagatt aaattaaaaa tcaaacaatc aaagctcgtc
      781 tgatataatc gatatattct gttaaacgaa aaataaatat gtttaaaaaa ttcaaaccgg
      841 tattgttgtc attttttgca cttgtatttg ccttttggct gggaacgggt attgcctatg
      901 agattaatcc gcgttggttt ttgagcgata cggcaactga aatacctgaa aatccgaatg
      961 cttttgtggc gaaacttgcc cgcctgttcc gaaatgccga cagggcggtt gtcatcgtga
     1021 aggaatcgat aaggacggag gaaaatcttg ccgaaactgt ggatgacggt ccgttgcagt
     1081 cggagaagga ttatcttgca ctcgctatcc ggctcagtcg tttgaaagaa aaggcgaaat
     1141 ggtttcacgt aacggagcag gaacatgggg aagaggtttg gctggattac catatcggcg
     1201 agggcggttt ggttgcggtt tcgcttttgc aacgctcgcc ggaagcgttt gttaatgccg
     1261 aatatctgta tcggaacgat cgtccgtttt ctgtaaatgt gtacggcgga acggctcacg
     1321 gagaaaatta tgaaacgaca ggagaatatc gggttgtttg gcaaccggac ggttcggtat
     1381 ttgatgcgtc ggggcgcggg aaaatcgggg aagatgttta tgagcattgt ctcgggtgtt
     1441 atcagatggc ccaggtatat ttggcgaaat atcgggatgt cgcgaatgat gagcagaagg
     1501 tttgggactt ccgcgaagag agcaaccgga ttgcgtcgga ctcgcgcgat tctgtgtttt
     1561 atcagaatat gcgggaattg atgccccggg ggatgaaggc gaacagtctt gtggtcggct
     1621 atgatgcgga cggtctgccg caaaaagtct attggagttt cgacaatgga aaaaaacgcc
     1681 agagtttcga atattatttg aaaaacggaa atctttttat tgcacaatct tcgacggtag
     1741 cattgaaagc ggatggcgta acggcggata tgcagaccta tcatgcgcaa cagacgtggt
     1801 atttggatgg cgggcggatt atccgcgaag agaaacaggg agacagactg cctgattttc
     1861 ctttgaactt ggaagatttg gaaaaagagg tgagccgtta tgcagaggct gcggcgagac
     1921 gttcgggcgg caggcgcgac ctttctcact gaacgcaaat gccgtctgaa accggttttc
     1981 agacggcatt ggagagtcaa acgctttttg caatgccgta tatgccgaat ccgattcgct
     2041 acaaattgaa aaccacaatc caaaaaacgg aaatacacat taaacggatc tttttaccga
     2101 ccgtatcatc ccataggttt tccacacctt ttttcatcca taaattcaaa ccatttaagt
     2161 ttgcatcctt accgacaatt gcagcagcat catccgcata acggtcaccc aaattagcca
     2221 aataacgcca accgccatag gtatcgccac gggaagcata accatacaag gtttggattt
     2281 gttctggtgt taaaacaact tcaccctcat tcataataag atcctttaaa ttatttttta
     2341 cttgaattgt ttacaaaact ctgtaccaat tgctctactt gttggtgata acctaccaac
     2401 tcctgctgtg tgagctgctg cttttcccga ataaacgggt aataccgtac cgggaaaccg
     2461 ttaatacagg aacctacgcc agtgctgtaa ttatcttctt cagtttggtt tattcgatta
     2521 ttccgatgag taataatcgc cggattttct ccttttttaa tatagtaaaa ttcatgaaaa
     2581 tatggatttt caggaatatg tcttttatct tcataagcct cgtattcagg ataggcagat
     2641 ggcatttttt taaccccgta agtaagcaat ctttctccca tagtcgtgct taaactacac
     2701 gcatcttttc ggataacaat atcgtccacg ctatccaaac cgtcgcgtag aaattggata
     2761 aagtgtgctt tgttttgctg gttgtatggc tcaagaaccc aaccttggtc ggtttttttg
     2821 taatagacgc taatctctat caaattaatg gagtaagcat cctgcccggg cgtgtcggaa
     2881 agccattttg tggcattgtc ttgcgtatac cgtgcaagat agccataggg gataaccatt
     2941 ttggtgccct gaaaatcaaa tgtaaccgta tgttcaaatc ctgtcattgg ctcgggattg
     3001 ttgaaactgg tttgttcatt aacaggttca catgaggtca tagtaaaaac actcccaata
     3061 aacaaaataa aaagttgata aatagtagta gcatatggtc tcataatttc aagcctagaa
     3121 attagttaaa gaatatatta tggtagatac taactcttta agttgacata ttgtgcgata
     3181 tcgagggctg taccgtattt tcccatcgta ttttcccatc gtattttccc gttctttata
     3241 tgagcggcag gcatcctcat aaaagaaggg ataccgataa ggaaaaatac cccagcataa
     3301 aaaatatact gattaatcaa tacaaatcca acaaacagtt catcgtattc ttttctgttg
     3361 gcgtataaaa aatactgata tatgccttca ctttcatagg gcgacgcttt atgtaccgtc
     3421 ctgtgtgttg aaacgtcggt tttccttacc gcctgccccg cttttccctg attgctgcca
     3481 actgccgtcc gaacttgaac cgtcaggttt cagacggcat tttttctgcc tgctcccaca
     3541 attgccgttt ttgtgcggcg gtcagttttt tgacggcgat ttcctgcctg agtgccgttc
     3601 ctttatccat cccgcctgca acgatacgca tcgccaccgg tttgaatacg cgggtatatt
     3661 tcgcgccttt accggttgtg tgggcggcaa gccgctgttg cggattcggg ctgatgccgc
     3721 aatagaacgc gctgttttca cacaatatca ggtaaacgct ccagtttgac gcgttcatct
     3781 gcctgcatcc tgagttttct ccccgttcca aggcgcaact ttcagcagca gcagcatacc
     3841 cggcagggca agtatgaaac acagctggaa aaacggtaca tacccgagcc attcaatcag
     3901 ataaccggca aaggaattga tgaccgtacg gggaacagcg gacaggctgg taaacagtgc
     3961 aagctgcgtt gcggtaaatg cgggattggt ctcgcgcgcc atatacgata caaacgccgc
     4021 cgtccccaaa cccacgccga ctgcctctgc gccgataact gccgccagca tcagcctctc
     4081 gcctgtgccg accgtgtcga aatgtccgaa ccctgccagc catacaaacc ccaaaacggt
     4141 tacagcctgc accgcgccaa atatccacaa ggctttgttt acgccgattt tcagcatcca
     4201 cacgccgccc aagatacctg ccgccactgc cggccacagt cctgcgtttt tggcaatcaa
     4261 accgatgtcg gtcttgctga aacccatatc cagataaaac ggcgttgcca acgcggttgc
     4321 catactgtcg ccgagtttgt aaaggaagat aaacagcagc acgcatacag ccgaagcgat
     4381 gcccttgcgt gtaaaaaatt ctttaaacgg ctctaccact gtctgcttca acgttttagg
     4441 aacggaaggc ggcaacacgg gttcgcgcgc gagaaacagc gtcatcagaa ggccgggcag
     4501 cataaataat gaagtgataa caaatacttc tgaccacggc atcctgtctg ccaacaccaa
     4561 actcaatgaa ccgggaatca gggcggcaat ccggtaggcg ttcacatgga ctgagttgcc
     4621 caaacccaat tcctcgtccg acaaaatctc gcgcctgaac gcatccaata caatatcctg
     4681 actggcggaa aaaaaagcga caagcaccga caagccggca atcaacggca gatgattccg
     4741 ggggtttaaa aaggcatagg ccgccaaagc cgccagcaac cctgcctgcg tcaacagcat
     4801 ccacccgcgc cgccggccca aaatgggaac cctgaccgcg tccatcagcg gagaccacaa
     4861 aaatttccaa gtaaacggca gaccgatcag cgccatcagc ccgatgctct tcaaatccac
     4921 ctgctcgctg cgcaaccatg ccggaatcag gttaatcaga aagtacagcg gcagccccga
     4981 ggtaaacccc gtaaaaatac agacaagcat atttcgggaa aagatttgcc cgataaaacc
     5041 tgatttcgat acagtcatgg ttcaaacagc caagaaataa aagcctattg tagcaaatat
     5101 cgcccaaccg ctgaaaaaca ccgaccctgc tccgccgttt tgccgttcaa atgcaaaatg
     5161 ccgtctgaaa ctttgttcag acgacatttc tactgaaacc tgagagtttt acaggctgta
     5221 atacatttca aattccagcg gatgcggtgc catgcggatg cggcggacat cttcctcttt
     5281 aaaggcgatg tagctgtcga tccagtcttt gctgaatacg ccgccgcgca ggaggaattc
     5341 gtggtcggct ttgagggcgg cgagggcttc ttccaaagag gcgcagacgg tcggcaccaa
     5401 tgcatcttct tccggcggca gatcgtacag gtttttatcg gcagggtcgc ccggatgaat
     5461 tttgttttga ataccgtcca aaccggccat caacaaagcg gcgaacgcca agtatgggtt
     5521 ggcggtcgga tcggggaaac gcgcttcgat gcggcgcgcc ttgctgctgt tcacggacgg
     5581 aatgcggatg gaagcggaac ggtttttggc ggaataagcc agtttggtcg gcgcttcaaa
     5641 gtgcggcaca aggcgtttgt aggagttggt ggacggattg gtaatcgcgt tcagggcttt
     5701 ggcgtgtttg atgatgccgc cgatgtaata aagggcggtg tcgctcaaac cggcatagcc
     5761 gtcgccggcg aacaggtttt gaccgtcttt ccagatggat tggtgaacgt gcataccgct
     5821 gccgttgtcg cccataatcg gtttgggcat gaaggtggcg gttttgccga agttgtgggc
     5881 aacgttttga atcacatatt tcatgtcttg ggtttggtcg gcgcgtttaa ccaaagtggc
     5941 gaagcgcgtg ccgatttcca tttggctgcc ggtaccgact tcgctgtggt ggacttcgac
     6001 tttgatgccg agttcttcca aaatgttcac catggcggag cgcaaatctt gaccgcagtc
     6061 aatcggggca acgggcgcgt aaccgccttt gacggtcgga cggtggccag tattttgacc
     6121 gtccaaatgc aagccgcttg accatgcgcc gctttcggaa gtgatttcgt aacgggtttt
     6181 gtgcatgtcg gtttcaaatt ctacgccgtc aaaaacgaag aattcaggct cggggccgaa
     6241 ataggcggtg tcgccgatgc cggaagattt caaataggct tcggcgcggc gggcgatgga
     6301 gcgcgggtcg cggtcgtaac cctgaccgtc ggcagggtcg atgacgtcgc aggtaatgac
     6361 gacggtaaca tcatcataaa aaggatcgac gaaggctgta gacgcatcgg gacgcagctg
     6421 catatcggaa gcctgaatgc ctttccagcc gccgatagac gaaccgtcaa acgcctgacc
     6481 gttttcgaac cactcttcgg ggtcttccaa cacgatgcgc gcaggcacgg taaagtggtg
     6541 ctgcttgcct ttggtatcgg taaagcgcaa atcgacaaag cgggcttcgc tttcttcaat
     6601 caattttacg gcgtttttaa tagacatttt tcagctcctg gaaaagggaa atagcaaaat
     6661 atgcggaacg cggcggcttg acgcaaagtc ggcatgattc taccacgtcc atgccgtctg
     6721 aaactcgccc tgcgtcaaat ttcaagctgg aaaatatagt ttcagacggc aagattgtca
     6781 acaatatgga taaaattgca tatcatgtcc ggatttctcc ccagcttata cagggaaaaa
     6841 ccgtaccgcc tgacaaccgt ttcagacggc atcgtttcca tctgtacgcc gccgttataa
     6901 aatagccttc ccgtcttttg gaacacgacc atgaccgccc tcccccgtta ctccgttttc
     6961 ggcaaccccg ttgcacacag taaatctccg cagattcatc ggcaatttgc ccttcaggaa
     7021 ggcgttgaca tcgaatacgg acgcatttgc gccgacatcg gcggtttcgc tcaggcggtt
     7081 tcgacatttt ttgaaacagg cggttgcggg gcaaacgtta ccgtaccgtt caaacaggaa
     7141 gcgtttcatc tggcggacga acattccgac cgcgctttgg cagcaggtgc ggtcaatacg
     7201 ctgattctgt tgaaaaacgg caagctgcgc ggcgacaata ctgacggtat cggtttggcc
     7261 aacgacatca cgcaggttaa aaacattgcc atcgagggca aaactatttt acttttgggc
     7321 gcgggcggtg cggtgcgcgg cgtgattcct gttttgaaag aacaccgtcc tgcccgtatc
     7381 gtcattgcca accgtaccca tgccaaagcc gaggaattgg cgcagctttt cggcattgaa
     7441 gccgtcccga tggcggatgt gaacggcggt tttgatatca tcatcaacgg cacatccggc
     7501 ggtttgagcg gtcagcttcc ggccgtcaat cctgaaattt tccgcgactg ccgccttgcc
     7561 tacgatatgg tgtacggcga agcggcaaaa ccgtttttgg attttgcccg gcaatcgggc
     7621 gcgaaacaaa ccgccgacgg actgggtatg ctggtcggtc aggcggcggc ttcctacgcc
     7681 ctctggcgcg gatttacgcc caatatccgc cctgttatcg aatacatgaa agccatgtaa
     7741 tatgttccgc atcgtcaaat ggctgattgc cctgcccgtc ggcatcttta tctttttcaa
     7801 tgcctatgtg tacggcaaca tcatcaccta ccgcgccgtc gcgccgcatc ggactgcctt
     7861 tatgtcgatg cggatgaagc agtttgaaca ggaaggtcgc gatgtcgcac tggattaccg
     7921 ctgggtaccc tacaaccgca tctcggtcaa cctgaaaaaa gccctgattg cttccgaaga
     7981 tgcccgtttc gccggacacg gcggcttcga ttggggcggc attcaaaacg ccatcaggcg
     8041 caaccggaac agcggcaaag tgaaggcggg cggttcgacc atcagccagc agcttgccaa
     8101 aaacctgttt ttaaacgaaa gccgcagcta tatccgcaaa ggcgaagaag cggcgattac
     8161 cgcgatgatg gaagctgtta ccgacaaaga caggattttt gaactgtatt taaactcaat
     8221 cgagtggcac tacggtattt tcggcgcgga agccgcgtcc cggtattttt atcaaatacc
     8281 cgccgccaaa ctgagcaaac agcaggcggc gaaactgacg gcgcgcgtcc ccgccccgct
     8341 ctactacgcc gaccatccga aaagcaaacg gctgcgcaac aaaaccaata tcgtgctcag
     8401 acgcatgggt tcggcagaat tgcctgaaag cgacacggac tgattgtttc agatatggaa
     8461 atgccgtctg aactgcggtt cggacggcat atgttttctg gtttttatag tggattaaca
     8521 aaaatcagga caaggcgacg aagccgcaga cagtacaaat agcacggaat cgattcactt
     8581 ggtgcttcag caccttagag aatcgttctc tttgagctaa ggcgagccaa cgctgtaccg
     8641 gtttaaattt aatccactat acctgcccga cagccgcaaa gaccgtatca tacatagcag
     8701 gcattgcggt gcgggattac tgccgcaaac ggcaggcaaa aaaaataacc gcttcaaggc
     8761 ggttttttgg tggccagagg cggaatcgaa ccgccgacac acggattttc aatccgttgc
     8821 tctaccaact gagctatcta gcctttatct gtcttgttaa gagatgcgta ttaaaccaaa
     8881 tttcgggaca acacgcaagc ggatttgtcg atatttttta tccaaccgac taagtcgatg
     8941 ttttaagttt aaattaattt cacagaaaaa tagtgcaaat cggttttttg ccgtttgttc
     9001 ggcgatattc tgcttttcag acggcaaaac gcttcaatat ttgaagttct tacccagata
     9061 aacagaacga acctgttcgt ttccgaccaa atcatcaggt tttcccgatg ccagcaccgc
     9121 accgtctgaa atgatgtagg cgcggtcgca gatgctgagg gtttcgcgga cgttgtggtc
     9181 ggtaatcagt acgccgatgc cgcgcgattt gaggaaaccg atgattttct ggatgtcgat
     9241 gacggcaatc ggatcgacgc cggcaaaagg ttcgtccaac aaaataaaat gcggtttcat
     9301 ggcgagtacg cgggcgattt cgacgcgtcg gcgttcgccg ccggacagcg acggcgcggg
     9361 gctgcggcgc aagtgtccga tattaaggtc ggcgagcagt ttttcgattt ccctgtcgat
     9421 ttgattttta tctttggttc tgatttccaa gatggcgcgg atgttttgtt cgacggtcat
     9481 tttgcggaat atcgaggctt cctgcggcag gtagccgaca ccgaggcggg cgcgttcgtg
     9541 tatgggcagg tgacgcaatt cctgcccgtc cagcatcacg ctgcccgcat cggcggcaat
     9601 gagtccgact atcatgtaga agctggtggt tttacccgca ccgttgggcc cgagcagtcc
     9661 gatgacttcg ccgctttcga tttcgaggga gaagctttta acgacttggc gttttttaaa
     9721 acttttttgc aggttttgaa caacaaggcg gctgacgttt gcgctcataa atatctcttc
     9781 aatatcttcg gtcggcatat gccgtccgaa ccgggtttat gtttcagacg gcattttggg
     9841 attattcgga tttttgcgta ctcgaaggct ggataacgac gctgaccctg ccggatttgg
     9901 aagcggattt tgcgccggat tttgtgctgc cgctgatggt atagacttcg gttttggtgt
     9961 tgtatgtaat caccgcacct tcggcgacat cgccgccgcg ctgtactttg gcattaccgg
    10021 ttaagactac ggtgctgcct gcagatgaat aagcaacgtt gtttgcctgt ccgcgcaccg
    10081 tgcctttgcc gccgtccaat gtctggctga agcggactgg cgaaccttcc gccctcacgg
    10141 attcgccgcc tttgccgccg cgtgtaacat tgacgcgggc ggcggaaata ttgagcgtac
    10201 cctgtctgat gacgacgttt ccgctgaatg tggtgctttg gttggcttga tcgagcgaac
    10261 cttggtcggc ctcaatctga ataggctgcc tgctgtcgct ttgaagggca aaagcggggg
    10321 acgccgaaaa aaatgctatt aaaacaaata gcttacatat cttttgtatc ataaatcgtg
    10381 gctttcactt tagatgagaa gttcaacatg cctgttttgt ggtcgtaagt catgccgccc
    10441 gcctgaccgt gcgatgcacc atattggaaa ctgacaggcg tatcggtttg ggcatattga
    10501 gattcggtat cgacgtgcag cttttcggct tcaactttac ccgcctgccg tttgccgtcg
    10561 gcggttttgg tcagcacaac gttgttttta aaaagaacct gtttgttttc ggtatggtaa
    10621 acggcttcgt cgctgccgac ttcgtacaac aacctgcctt cttggaagaa cacgagatgc
    10681 ggcgaatcaa aatggatgtc gctgctttcg ggaaactgtt tcgcgccttt cgcgctcaaa
    10741 tgttctttta agtatccctg ttcgtcaaac cgcctgccat ccaatccgtc cattgtgtat
    10801 tgcggttcgt cgggattgag cctgacttct tcaatctcga cttcgctgat gcgtcccaac
    10861 catgccgaca ggctgcccaa ggcaaccgcc aatatcaatg ggaacgcaat tccgtaccgc
    10921 catcttattt tcatttgatg tactcgttca aagccgcgcc caaagtccct tgcgcctgca
    10981 taatcaggtc gcacacttcg cgcaccgcgc ccgcaccgcc ggggcgtttg gcaatatagg
    11041 cggcgtgttg cagcgtaaac caatgcgcgt cggggacggc aaccggcaat ccgcaacgca
    11101 ccatcaccgg caaatcgacc acgtcgtcgc cgacaaaggc acactcggct tcttccacgc
    11161 cgacctgagc gcgcagctct tcatacgcgg cgcgtttgtc cgaaatgcct ttgaagtagt
    11221 aatttatgcc caactgtttg acgcggatgc cgacggaggg cgcgtcccga cctgtgataa
    11281 tcgcagtctg cacgccgctt gcctgaagca ttttcagacc gtgtccgtcc agtgtgtgaa
    11341 acgatttgat ttcttcgccg ttatcgcgga taaagatgcg cccgtcggtc aaaacgccgt
    11401 ccacatccag gatcagcagt ttgattttgg cggcgcgcgc ctgtaattcg ggagaaattg
    11461 cctgcatggg atttccttgt tttcagacgg catatcttac cacaaatgcc gtctgaaacg
    11521 gctgcaacgc cttatacaat ccgcgccgcc aaaaggtcgt gcatattcag cgcaccgatc
    11581 agcacgccgt ctgcatcggt aaccagaagc ccgttcacat ggtttgcctg catgactttc
    11641 agggcttcgg tggcgaggcg ttcggcggcg atggttttag ggtgtgtatg catgacttcg
    11701 tctatcgaaa gaccggtaaa attgtcgcat tcttgaaaca ggcggcgcaa atcgccgtcg
    11761 gtgaatacgc ctttcagacg gccttgcccg tccgttaccg ccagcatccc cagccctttt
    11821 tcactcatgc tgacgatggc ttctttcaag ggcgtgccga gtcggacggc aggcaggccg
    11881 ccgcctttgt gcataatgtc ggcaacgcgc aaaagtaggc gtttgccgag gctgccggca
    11941 ggatggctca aggcgaaatc gtcgggcgtg aacgcgcgtg cgcgcagcag gacgaccgcc
    12001 aacgcatcgc ccaaagccat gacggcggtg gtgctggtgg tcggggcaag ccccagcggg
    12061 caggcttctt tggaaaccga cgccgtgatg tggatgtcgg catggcgcgc catggttgaa
    12121 tcggggcggg cggtgatgca gacaagcgtg atgtctttgc gtttgagcgc ggggatgatg
    12181 gcggcgattt cgtcgctttc gccggaattg gaaatcgcga cgaccacgtc gttgtccaca
    12241 atcatgccca aatcgccgtg tgccgcttcc gcagggtgga cgaaaaacgc gggcgtgccg
    12301 gtcgaggcca tggttgccgc cattttgcgc ccgatatgtc ccgacttgcc catgcccgtg
    12361 ataacaaccc tgcccttgca gtgcaacaac gcgtctgccg caaggacgaa gttttcgtcc
    12421 aattccgctg caatttcgcg caagccttcc gcttcggtgt gcaacacttc gcgcgcccag
    12481 tcgagatatt ttccgttttc tgccattgcc atccgccttt gctgtaacaa aactacccaa
    12541 aatttccgaa tcggggtagc ataagcattc aaaccccgcc atccatccaa aggaatcaga
    12601 aatgactatt ttatcggacg ttaaagcatt aggacaacaa atctggctgg acaacctttc
    12661 ccgctcgctc gtgcaaagcg gcgaattggc gcaaatgctg aaacaaggcg tgtgcggcgt
    12721 aacttccaat cccgccatct tccaaaaagc cttcgccggc gacgcgcttt acgccgatga
    12781 aatcgccgcc ctcaagcagc aaaacctcag ccccaaacaa cgctacgaaa ccatggcggt
    12841 tgccgatgtg cgtgccgcct gcggcgtttg ccttgccgaa cacgaatcca ccggcggcaa
    12901 aaccggtttc gtcagcctcg aagtttcgcc cgaattgtcc aaagacgcgc aaggcacggt
    12961 agaagaagcg cgccgcctct acgccgccat cgggtgcaaa aacgcgatga ttaaagtgcc
    13021 tgccaccgac gcaggcatcg atgcgctcga aatcctcgtt tcagacggca tcagcgtaaa
    13081 cctcacgctg ctgttctcac gcgcccaaac cctcaaagcc tacgccgcct acgcgcgcgg
    13141 cattgccaaa cgcttggcag ccggacaaag cgttgcccat atccacgtcg tcgccagctt
    13201 cttcatctcc cgtgtggacg gcgcgctgga cacaacactg cccgaccggc tcaaaggcaa
    13261 aatcgccatc gcccttgcca aagccgccta ccaagactgg gcgcaatact tcggcagctc
    13321 ggaatttgcc gcgctggaag cccaaggcgc aaaccgcgtg cagcttttat gggcatctac
    13381 cggcgtgaaa aacccggcct atcccgacac actctacgtt gacagcctga tcggcgcgca
    13441 caccgtcaac accgttcccg atgccacgct caaagccttt atcgaccacg gcacagccaa
    13501 agccacgctg accgaaggcg tagaagaagc acaagcgcag ctcgccgaaa ccgccgcgct
    13561 cggcatcgat gtcgaaacct tggcggcacg tttgcaggaa gacggtttga aacagtttga
    13621 agaagcgttt gaaaaactgc tcgcaccgtt ggtttaaact tttcatcccc gatgccgtct
    13681 gaaaggcttt cagacggcat cgcccttttg accggacaca gtgcggcata tcaaggattc
    13741 gccctaaaaa tattaatcaa caagaagtta atgcacgaca ttacccacca acacaaaagg
    13801 aaatagaatc atggcaaaag cactcgaaat catttcaccc aatgaaattt attcagatct
    13861 gatttttaag gatccggtac ctccccatac aatatataca gaattgacga aactggttag
    13921 tagaaatgca agtaatgaga gaattgcaga tagagccttt gattttttct cgtccggcct
    13981 tatggatggt tcagctaccg aagagcaata taatgcccta tatgatttaa ccttgcttga
    14041 agagcccggg atggaactga ataaagatga gataatcgct ttgattaata gtcttaaata
    14101 aaggggctgt accagatatg tacacaggac gttttgcccc cagcccgacc gggctgctcc
    14161 acatcggctc gctgctgacc gccgtcgctt cctatgccga tgcacgctca aacggcggca
    14221 aatggctggt ccgcatggaa gacctcgatc cgccgcgcga aatgccgggg gcggcaagcc
    14281 atatcctgca cacgcttgag gcattcggat tcgagtggga cggagaagtc gcctatcaga
    14341 gccgccgtta cgccctgtat gaagaaacac tatgccgcct gaaaaccgcc ggactggtct
    14401 atccctgcca ttgcagccgc aaagactggc agaccggggc aaggcggggc gcagacgggt
    14461 tcgtctataa cggacgctgc cgcaatccgc agcaacgccc tgcactgcaa ggcaaacagc
    14521 cggcgtggcg catccgcgtc cccgaccgcg acatcggctt ttcagacggc atcgtcggcg
    14581 gttacgccca aaacctcgcc cgcgacatcg gcgatttcgt cctgcttcgt gcagacggtt
    14641 actgggcata ccaactcgcc gtcgttgccg acgatgccga acagggcgtt acccacatcg
    14701 tccgcggaca agacctgctt gtttctacgc cgcgccaaat ctatttgcag cagtgtttgg
    14761 acgttccgac accgcaatat gcccacctgc cgctgctgac caacgcacaa gggcagaaat
    14821 ggtcgaaaca gacgctcgca cccgcattgg atttaaaccg ccgcgaacaa ctcctccgcc
    14881 aagtgttccg ttacctcaac ctgcccgaag caccggaagc cgaccgccct gccgaactgc
    14941 tcgactgggc ggtggcacac tgggatatgg gcaaagtgcc gaaacacgcc attaccgccc
    15001 cctaaacacc gcccgacaaa cgcacaaatg ccgtctgaaa ccttcagacg gcatttcata
    15061 cacacagccc cgccctattc gcgcatattt gcccgttcga cttctttcca cgcttcgaga
    15121 atcaggccgc cgtcgttgtc tttcaagagc gttgcatcgg aaagcatacg ccgccaagtg
    15181 cgggcatttt tcagaccgtg catcagcccg aggctgtggc ggacgatgtg gcgcaagatt
    15241 gtgccgcgtc cggcttggat ttgggcttga ctgtatgtgt agagacgctg caccaaatcg
    15301 gcgtattcaa tcgggctgcg ggtatcgccg taaaacagcc tgtcccattc gcgcatcacc
    15361 atcgggttgt ggtacgcctc gcgcccgacc atcacgccgt caacgtgttg caggtgttgc
    15421 gcgatttcct cattggtcgt gatgccgccg ttgatgatga tttccagccc gggaaactct
    15481 tgcttgaggc ggtaaacata atcgtatttc aagggcggaa cgtcgcggtt ttctttgggt
    15541 gaaagaccgt ccagccacgc gttacgtgcg tggacgataa aggttttgca ggcggttttg
    15601 tcgcgcagcg tgccgacgaa atcggcaacg gtttggtatt cggtctgcct gtccacaccg
    15661 atgcggtgtt tgacggtaac gggaatcttg accgcatcct gcatggcgtt gaggcagtcg
    15721 gcaaccagcc cgacttcgtt catcagacac gcgccgaacg cgcctttctg gacgcgcgga
    15781 ctggggcagc cgcagttgag gttgacctcg ttgtagccgt attcttcggc ggctttggcg
    15841 gctttcgcca aatcggacgg gtcgctgccg cccagttgca gggcgacggg ctgctcgcct
    15901 tcgttaaaca tcaaaaagcg gtctttgtcg ccgtaaacaa tcgcgccggc gttgaccatt
    15961 tcgctgtaca gccaagtatt gcgggtaatc tggcgcgcca tataacggta gtgcctgtcc
    16021 gtccagtcca acatcggggc gacggacagg cggcggggag gaagggtttg cgtgttgtcg
    16081 ttcattgtcg atatttcacc attgcggatt caatttcaat ccggaacaac cttcattttt
    16141 ggggaaatcc gcgtcatgaa agagtccagc agtatggtta ccggcccgtc gttgcagagc
    16201 gacacctgca tatgcgtgcg gaaacgccct gtttcgacat gaattccgtg tccgcgcaac
    16261 agttccgccg ttcgcaggta aagctgctgc gcctgttctg caggtgcggc ttgcgaaaac
    16321 gaaggccgcc gcccgcttgt cgcgtcggca taaagcgtaa actgcgacac cagcagcacc
    16381 gcgccgccga catctttcaa agacaggttc agcttgcccg cttcgtcttc aaacacgcgc
    16441 aaatgggcga ttttgtcggc gatatagcgc gcatcttttt ctgtgtcact atgcgttacg
    16501 ccgagcagca cgacgaatcc gccgtcgatt ttgccacagg tttccgtgcc gttttcggac
    16561 acgacatcca cctttgcacc taccgttttc tgtatgaccg cacgcataag ccgtcctcct
    16621 tacctgaacg tgcaatgccg tctgaaaagg cttcagacgg catcggtatt ggaaatgcca
    16681 ttacggtttg acaccgtttt cttccaaaat ggcatcgatt ttcaagcggg ctttttcctg
    16741 tctcaaacct tgttccaact ggtttctgac caactcgaaa ggctgcgcgt cggggttttt
    16801 cccgacctcg ctgagtttga acagataata gcgttcgccc aatttgaccg gatcgcgggt
    16861 aacgtcgccc cgattcatcg cggcaaactg cgaagccagc ggctcgggaa gctgctgcgc
    16921 cataatgaaa ccgtcaaaag cctgctcgtc gttcggataa cgcttcatca gcccttcaaa
    16981 agacagccct ttgagcagga gctgctgcgc ctgacgcgcc tcctcttcgg ttgcgaagct
    17041 gacctgctgc aatttgatca tgcggatttg ccgctcataa aactgacgca gtgcgctttc
    17101 ggaaaccgtt tccgaacgtt ccagaaaacg gacgtactcc tcggcataaa aagacgcttc
    17161 ggcgatttta aagcggtttt ggacatcctt atccttatcc aaaccttcct tcaatgccct
    17221 gtttttcaaa acttccaaag tttgcagccg acggacggca tcgtttcgga ttgcctgccc
    17281 gtccggtttt tgggactgct ccgcatgccg gtctgcctgc tgcatgatct gcgccaccag
    17341 cgtatccacc aaagccgggt cgatttcggg tgctttggct gccgcaaaac ctgccaacat
    17401 tgcagcaata actgcggcag cggttttttt ctgtttcatg gtattccctg tcctgcgcgg
    17461 cggcaaaacg cctgtctgaa cgtcttgccg ccatatcccg ttttcagaat tattttgcag
    17521 gtttgatgtt tgccttgccc aacagtgcac cgacggcacg gtcaatccgt tccgcctgaa
    17581 ggttgcccgc aatctgtcct ttcatttcat caaaagaagg cacttttacc tcgcggctgt
    17641 cgttgacata ataaacgccg tagaaatcgc cgtttttcag cggcgttgcc gtaaattcgc
    17701 cttttttcaa gtccttaatt gcctgataaa gcggcggaac accctgttcc aaatctttca
    17761 gcggcacata tccgaccggc gcaccggtct gtttggtacg gtcgttgagg gaatattgtt
    17821 tcaagacggc atcgaaacct tttttcgcct tcaagtcggc aaccgctttt tttgcatttt
    17881 cttccttgtc ggtcaggatt tcgcccaact ggacttcctg cgtacctttg taaaaaccgc
    17941 tgatattgtc atatgcggct tttacttcct gctcggaaac cggttgggtt ttggcgatat
    18001 gcaatgcgta tgcctcgccg ttcaagccat attttaccgc ctgccaaacg gttttgaagg
    18061 acggtttctt gtcgtcgccc gactttttcg cttcggcacg caatttggca agcgcatttt
    18121 taaactctgc cgaccggtcg agtttcaggc gtttcacttc ctgtgcgacc acggtgttga
    18181 ccacttcgtt ttccagcagg gattggcgca gttgcggcgt gtcttcggca cggctgtttt
    18241 ccgcacggaa tgcggcaacc tgcgcatcaa tgacggaact gtcgattttc tgaccgttga
    18301 cggttgccag cgtttgggca aacaggctgc cggaacaggc aagcagtgca acggaagtca
    18361 ggatttttgc tttcataatc ttctctcttt aaattttaaa ttgaaatgtt cgggcagact
    18421 tcattcagtc cgccgtatgg aaatactcgt caggggtagc cgcccggatg ccgagcgcgt
    18481 gaatcatgcc gcctgaaaac aaatctttga gcagcgattt gaccgttttc tggcggttca
    18541 ggcggcttac gccttcaaaa cggccgctga cgaccaaaac ggcataatgt ccgccgccgg
    18601 tattgcccgc gtgtcctttg tgcagatggc tctcatcgcc gatttccaac accagcggat
    18661 cgagcgtctg caggcgttcg cggatcaaat cgacggccgg cattatttga acaccgcttt
    18721 aaacggcttg atcagcactt cgctgtacac gcctgcatga acatagggat cgtcttccgc
    18781 ccacgcctct gccgcatcca aagactcgaa ctgcgccaca atcaaactgc ccgaaacgcg
    18841 gttggagtcc tccggcaaag gattcgggcc tgccgtcaac agccggcctt ccgacttcag
    18901 cgtctccagc cgtttgaggt gttcgggacg tgccgccata cgcgcttcat acacattctc
    18961 cccgtctgtt gccagcaaca taaaatattc cacagtcaat cctccttttt cagacaggta
    19021 ctcagataaa taccctgaat aataaagaaa acaagcatca gtgcagtcga gccgaacatt
    19081 ttatagttga cccattgcga ctcgaaccgg gtaaacacaa accagttggc gatacccata
    19141 aaaatcagga aaccgaccca catatacgtc aatttcgccc atacggcatc cggaagctga
    19201 atctccctgc cgatactcgc cttcaagccg tttttaccgg cgaggtggct gccccacagg
    19261 aacagcgcac ccagccaaaa caaaacgctc ggcttccaca taatgaagcg gctgtcgccc
    19321 aaaacaatgg ttgcgccgcc gaatacgaca atcagcacca gtccgaccca ctgcatcgta
    19381 tccagctttt tatatttcca atacagaaaa gccgcctgaa ccacaccggc aaccaaggca
    19441 accgccgtag cggcaatcat atttttggta acggtataag tagcaaaaaa caagatgaca
    19501 gacaaaaggt cgctgacgaa tttcatatcc aaatgagaaa gcaaatcaaa taaacgctaa
    19561 tcatacaggc agaaccctgt ttttgcacct gcaaaaccaa caaattttgt tttgattgat
    19621 gcaaatatgc acagatgttt ttgaaaaaag atggagatat gtcataattt gtaaaaacgg
    19681 cgaatgattg ccgtttaaaa tgtggcggcg gtcggtacat tcacatatta aatgcccgcc
    19741 ggccgactgc cccgccgatg cccgatgatg acgaaattta cagactgtac gcggtcaaac
    19801 cgtattcagc cgccaaccca caggggatac atcttgaaaa acaacagaca aatcaaactg
    19861 attgccgcct ccgtcgcagt tgccgcatcc tttcaggcac atgctggact gggcggactg
    19921 aatatccagt ccaaccttga cgaacccttt tccggcagca ttaccgtaac cggcgaagaa
    19981 gccaaagccc tgctaggcgg cggcagcgtt accgtttccg aaaaaggcct gaccgccaaa
    20041 gtccacaagt tgggcgacaa agccgtcatt gccgtttctt ccgaacaggc agtccgcgat
    20101 cccgtcctgg tattccgcat cggcgcaggc gcacaggtac gcgaatacac cgccatcctc
    20161 gatcctgtcg gctactcgcc caaaaccaaa tctgcacttt cagacggcaa gacacaccgc
    20221 aaaaccgctc cgacagcaga gtcccaagaa aatcaaaacg ccaaagccct ccgcaaaacc
    20281 gataaaaaag acagcgcgaa cgcagccgtc aaaccggcgt acaacggcaa aacccatacc
    20341 gtccgcaaag gcgaaacggt caaacagatt gccgccgcca tccgtccgaa acacctgacg
    20401 ctcgaacagg ttgccgatgc gctgctgaag gcaaacccaa atgtttccgc acacggcaga
    20461 ctgcgtgcgg gcagcgtgct tcacattccg aatctgaaca ggatcaaagc ggaacaaccc
    20521 aaaccgcaaa cggcgaaacc caaagccgaa accgcatcca tgccgtccga accgtccaaa
    20581 caggcaacgg tagagaaacc ggttgaaaaa cctgaagcaa aagttgccgc gcccgaagca
    20641 aaagcggaaa aaccggccgt tcgacccgaa cctgtacccg ctgcaaatac tgccgcatcg
    20701 gaaaccgctg ccgaatccgc cccccaagaa gccgccgctt ctgccatcga cacgccgacc
    20761 gacgaaaccg gtaacgccgt ttccgaacct gtcgaacagg tttctgccga agaagaaacc
    20821 gaaagcggac tgtttgacgg tctgttcggc ggttcgtaca ccttgctgct tgccggcgga
    20881 ggcgcggcat taatcgccct gctgctgctt ttgcgccttg cccaatccaa acgcgcgcgc
    20941 cgtaccgaag aatccgtccc tgaggaagag cctgaccttg acgacgcggc agacgacggc
    21001 atagaaatca cctttgccga agtcgaaact ccggcaacgc ccgaacccgc tccgaaaaac
    21061 gatgtaaacg acacacttgc cttagatggg gaatctgaag aagagttatc ggcaaaacaa
    21121 acgttcgatg tcgaaaccga tacgccttcc aaccgcatcg acttggattt cgacagcctg
    21181 gcagccgcgc aaaacggcat tttatccggc gcacttacgc aggatgaaga aacccaaaaa
    21241 cgcgcggatg ccgattggaa cgccatcgaa tccacagaca gcgtgtacga gcccgagacc
    21301 ttcaacccgt acaaccctgt cgaaatcgtc atcgacacgc ccgaaccgga atctgtcgcc
    21361 caaactgccg aaaacaaacc ggaaaccgtc gataccgatt tctccgacaa cctgccctca
    21421 aacaaccata tcggcacaga agaaacagct tccgcaaaac ctgcctcacc ctccggactg
    21481 gcaggcttcc tgaaggcttc ctcgcccgaa accatcttgg aaaaaacagt tgccgaagtc
    21541 caaacaccgg aagagttgca cgatttcctg aaagtgtacg aaaccgatgc cgtcgcggaa
    21601 actgcgcctg aaacgcccga tttcaacgcc gccgcagacg atttgtccgc attgcttcaa
    21661 cctgccgaag caccgtccgt tgaggaaaat ataacggaaa ccgttgccga aacacccgac
    21721 ttcaacgcca ccgcagacga tttgtccgca ttacttcaac cttctaaagt acctgccgtt
    21781 gaggaaaatg cagcggaaac cgttgccgat gatttgtccg cactgttgca acctgctgaa
    21841 gcaccggccg ttgaggaaaa tgtaacggaa accgttgccg aaacacccga tttcaacgcc
    21901 accgcagacg atttgtccgc attacttcaa ccttctgaag tacctgccgt tgaggaaaat
    21961 gcagcggaaa tcactttgga aacgcctgat tccaacacct ctgaggcaga cgctttgccc
    22021 gacttcctga aagacggcga ggaggaaacg gtagattgga gcatctacct ctcggaagaa
    22081 aatatcccaa ataatgcaga taccagtttc ccttcggaat ctgtaggttc tgacgcgcct
    22141 tccgaagcga aatacgacct tgccgaaatg tatctcgaaa tcggcgaccg cgatgccgct
    22201 gccgagacag tgcagaaatt gctggaagaa gcggaaggcg acgtactcaa acgtgcccaa
    22261 gcattggcgc aggaattggg tatttgattc ccaactgccc tttcgcagat caaggatgcc
    22321 gtttcagacg gcatcttttt tgccttatcg gtgtaacgga taaagtttga accggcacag
    22381 gctcaaacag caggccgacg gcaacaaaat gccgtctgaa acccctaaag gcttcagacg
    22441 gcattggcgg cggcggtatc aggcagtttg ataggcaacc gaatcgtcac cgcttttctt
    22501 ttgaatgaac tcgattttgt atccgtcggg gtcttcgacg aaggctatca cggttgtgcc
    22561 gtgtttcatc gggccggctt cgcggacgac gtttccgccc tgccgcttca cacgttcgca
    22621 ggcttcgtag gcatcgtcca cttcaatcgc gatgtgtccg taagcgttgc ccaagtcgta
    22681 tcgttccgta tcccagttgt gcgtcagttc caaaaccgtg ctgtcggttt catcgccgta
    22741 accgacgaag gcaagggtaa atctgccttc gggatagtct tttcggcaga gcagtttcat
    22801 gcccaaaacg ttttggtaga aatcaaggga tttttcgaga ttgcccacgc ggagcatagt
    22861 atggagtaag cgcatttttc gtgttcctca ttggtggtgg ttaacttcga tttagtggtg
    22921 gttaaacttc aattttattc ggggtaaacg tctgccattt gttgcaggcg ggacagtgcc
    22981 agaaaaagac ttgggatttg aagtggcagt tgcggcaacg gtacatcacg ctgcgctgta
    23041 gctgccgtcc gataaccgaa cgcatcatgt cggcatcggc tttccaagcc ggattcatat
    23101 cgctgagttt caaaccgagc aggcggtaca cgccgttaag gtcgggcttg cggcggacaa
    23161 gctcgacggc ggtttgcgcg gcttctttct cgcccttaag cagcagggat ttctcgtaca
    23221 cgacattgat caggtcaagt tcgggaaacg tctgcatata tcctgtcaga cggttcaagc
    23281 cttcttcagg ttttccctgc gcggcatagg cttcgtaaag cttctcgccg accatgctca
    23341 agtatgcatg gttttgctgc tcgatggcgg cataggcttc gacggcggca gggaaattgc
    23401 cttgtcggtg ttcgatgtcg cccaaaatca tgttggcgcg ggtgcatttt ttgttggctt
    23461 cgagtgcctt gccgacattg aaacgcgcga catcgaaatt ggacttgaac agcgcggctt
    23521 gggcaagttc gcaataaaac tgggcaattt caaactgata ggtctgatcg tcatggctga
    23581 gcagccgggc ggtttcaacc gctttttccc aatccctgtc ctgttggtag atattgagca
    23641 ggtgctgtct ggcttcacgc gccatttcac cgtcttgcag ccccaaaaaa atctgttcgg
    23701 cacgatcgac caaacccgcg ctttggtagt tttgcgccaa ttcaaacagg acgcgcgcgc
    23761 gcttttcgcc gaccgtatcg ggagaatcga gcattgtccg gtgtatgttg atggctttgt
    23821 cgttttcgcc gcgctggcgg taaagtttgc cgagggtgag gttcaaatca tacgattgcg
    23881 gccggccgtc gacgacttcc gccaactccc ttgccgcgcg cccgctgttg cggtcgacca
    23941 aagcgtccaa gcttttataa aatcccgaag ggatgctttt tgcctgcttc aatacggttt
    24001 tcatatccac gcgggcggca aaccagccca tcgcgaagaa gacgggcaaa aggataatcg
    24061 gcagcaggat aatccacaat tcgttgtcca tatcggcttt cttaaggctg tttggtagat
    24121 tcgggcgcat tttgcgccgg tggtgcggtc agctccttcc ccgtcaaacg cgcatttttc
    24181 tttacttcgg cacgcaacct gccgttctcg ccacgtaacg acaacaaccg tccgaacaag
    24241 gcaaacattc caaaaataat accgactaca aatgcgccga acaatacgac aatcagcggc
    24301 aaatcgaatt tttgccccgg caggtaggaa aaggtaacgg catccgtatt aataacggca
    24361 agcagcagga agagcagcag gataatgatt ttgatgaagg tatagataag tttcatgttt
    24421 gctccgtaaa taaaaacgcc gcccggacag cttcgcactc tgaaccaaga ttgcgctagt
    24481 ttaaacgatt tgcacggttt cggataggat gcggcagcgt gttcagacgg catacggagt
    24541 atgcgattgc cgacaatttt accaaataca tcgttccttt ccatttgaaa aataacggat
    24601 tggacaccgc atcgacagaa aaacccgccg cgcacttgtc aaaaccctgt ttgcaggcgt
    24661 atctttacaa tcttcaaatt caaaccgttc attgaaacat atcagaataa gaaaggcttt
    24721 acatcatgag cagacccgta cccgccgtat tcggcagcgt ttttcacagt caaatgcccg
    24781 tcctcgccta ccgcgaaggc aaatggcagc cgaccgaatg gcaatcttcc caagacctct
    24841 ccctcgcacc gggtgcgcac gccctgcact acagcagcga atgtttcgag ggactgaaag
    24901 ccttccgtca ggcagacggc aaaatcgtac tgttccgtcc gactgccaac atcgcgcgta
    24961 tgcggcaaag tgcggacatt ttgcacctgc cgcgccccga aacccaagcc tatctcgacg
    25021 cgctaatcga attggtcaaa cgcgccgccg atgaaattcc cgatgcgcct gccgccctgt
    25081 acctgcgtcc gaccttaatc ggtaccgatc ccgttatcgg caaggccggt tctccttccg
    25141 aaaccgccct gctgtatatt ttggcttccc ccgtcggcga ctatttcaaa gtcggctcgc
    25201 ccgtcaaaat tttagtggaa accgaacaca tccgctgcgc cccgcatatg ggccgcgtca
    25261 aatgcggcgg caactacgct tccgccatgc actgggtgct gaaggcgaaa gccgaatacg
    25321 gcgcaaacca agtcctcttc tgcccgaacg gcgacgtgca ggaaaccggc gcgtccaact
    25381 ttatcctgat taacggcgat gaaatcatta ccaaaccgct gaccgacgag tttttgcacg
    25441 gcgtaacccg cgattccgta ctgacggttg ccaaagattt gggctatacc gtcagcgaac
    25501 gtaatttcac ggttgacgaa ctgaaagctg ctgtggaaaa cggtgcggag gcgattctga
    25561 ccggtacggc agccgtcatc tcgcccgtta cttccttcgt catcggcggc aaagaaatcg
    25621 aagtgaaaag ccaagaacgc ggctatgcca tccgtaaggc gattaccgac atccagtatg
    25681 gtttggcgga agacaaatac ggctggctgg tcgaagtgtg ctgatgcttt aaataaaaaa
    25741 tgccgtctga aacccgtttg gcgtttcaga cggcattttc gcgttcggac catttccgct
    25801 gcacctgcag caagtcgggc atttccctag aaatacgctt cagacggcaa accgccgccc
    25861 gaaaccgata cgcggcacgg acggcgcacg gatttgaaaa cggcggatta tccctcggtg
    25921 ctcaaggcat tgatgctgta accgccgtca acgtaagtga tttcgccggt aatgccggac
    25981 gacaggtcgg acagcaggaa ggcggcggca ttgccgactt cttcgatggt aacgttgcgg
    26041 cgcagcggat tgtgggcggc gacgtgtccc aagagtttgc cgaaatcggc gatgccggag
    26101 gcggcgagtg ttttgatcgg accggcggaa ataccgttgc agcggatgcc ttctttgccc
    26161 aaacaggcgg cggtaaagcg gatgcctgcc tcaaggctgg cttttgccat acccatcacg
    26221 ttgtagttcg gaatcgcgcg caccgcgccc aagtagctca gggcgacgat ggcggagttt
    26281 ctgccgcgca tcatcggacg ggcggctttt gccagcgcag gtaggctgta tgcggaaatt
    26341 tcgtgtgcgg tgttgaacgc ttcgcggctg atgctgtcga ggaagtcgcc gctcaaggct
    26401 tctttcggcg caaaaccgat ggaatgcacc aaaccgtcca agccgtccca atgtttgccc
    26461 aaatcggcga acacttggtt gatttcgtcg tcgctggcga catcgcagcg gaacacgagt
    26521 tcggaatcca attccgccgc cattttgcgg acgcgctctt ccagtttgtc cacaacgtag
    26581 gtaaacgcca gttccgcgcc ttgttcgcgg caggctttgg cgatgccgta agcgatggaa
    26641 cgctcggaaa tcatgccggt aatcagaatt tttttgcctt gcagaaaacc cattttgtat
    26701 ccttcaaata gtgttcggtt cgttctaaac cgttgattat agcaaattgt ccctgtttct
    26761 gtgttttcac gttgcagcat gcaaacggca atgccgtccg aagcggattt cagacggcat
    26821 cggacgtttc aaatacggtt taaggcatca gatgccgcgc aacaattcgt tcacactggt
    26881 tttcgcacgg gtttgcgcgt ccacgcgttt gacgatgacg gcgcagtaaa ggctgtggct
    26941 gccgtctttg gaaggcatac tgccggacac gacgaccgaa cctgccggta cgcggccttg
    27001 gtagatttcg ccggtggtgc ggtcgaagat tttggtggat tgaccgatga acacgcccat
    27061 cgaaatcacg ctgccttctt cgacaatcac gccctcaacg atttcagaac gcgcaccgat
    27121 gaagcagttg tcttcaatga tggtgggggc ggcctgcagg ggttcgagta cgccgccgat
    27181 accgacgccg ccactcaagt gcacgttttt gccgatttgc gcgcaagagc cgacagtcgc
    27241 ccaagtatcg accatcgcgc cttcgtcgac gtatgcgccg atgttgacgt aagacggcat
    27301 cagcacgaca tttttcgcca caaagctgcc gcgtcgggca accgcacccg gaacggcgcg
    27361 gaagcctgcg tttttgaact cgtcttcaga ccagtcggca aacttggtcg gcactttgtc
    27421 gaagtatttg ttcacgccgt cgttgaggac ttcgttgtct tggatgcgga aggacagcaa
    27481 cacggctttt ttcgcccatt cgttgacttt ccactcgccc acgcccaagc attcggcaac
    27541 gcgcagtttg cctgaatcga gttggcggat ggtttccaac acggcttctt taacttcggg
    27601 agcaacagtg gacggggtga tgtccgcgcg gttttcaaag gcggtttcga taatgttttg
    27661 caaagacata atgtttcctt atgtgagatg tttcgtggga cgcttgccgg tattttcaag
    27721 cagtatgttc atttgaaatg aatgcgctat tgtgccacgc aaacgcatat tcgtcagcaa
    27781 tacgcgggcg gttgccgaac attggcgttt cggaatcggg cgggcggcag gtattgcctt
    27841 ttccgcaccc gaatcaatac agaacggcgg cggtactttt atgccgcgcc gacgcctttc
    27901 agacggcatt cacggtaaaa cgccatcagc ccttcggtcg aggcatcgtg tacggacgtg
    27961 ccgccttcca gttcgccgat gatggttttc gccaactgtt tgccgtactc aaccccccac
    28021 tgatcgaagg gattgatgtc ccaaattaca ccctgcacaa aggttttgtg ttcgtaagcc
    28081 gccatcagca tacccagatt gtagggcgtg aggcggtcga gcaggatgct gttactgggg
    28141 cggttgccgg ggaactcttt gtgcggcgcg aggcgttcgc gttccgcttc gggcaaatct
    28201 gccaattcgg cgcgtgcttc gtccaaggtt ttgcccttca tcagggcttc cgcttgggca
    28261 aaggcgttgg caacggtaaa acggctgcgt ccgtcctcca cgccctgcgc cgtcatcggg
    28321 acgataaagt cgcagggaat caggcgcgtg ccttgatgaa gcagttggaa ataggcgtgc
    28381 tggcagttga ccccttcacc gccgaacacg atgccgcccg ttttgcacac ggcgggactg
    28441 ccgtctgaag cgcggctttt gcccaaactc tccatatcga gctggttcag ccacaccggc
    28501 agcaggcgca ggttgtggct gtacggaacg gcggtctgcc cgtccgcgtg ttggaaattg
    28561 ttataccaca cggcaatcag tgccatcaga acgggaatgt tgcggcgcgg cggcgtgtgg
    28621 aaaaaatggc tgtccatcgc gtgcgccccc gccaacaact cgcggaaacg cgccccgccg
    28681 accgcaacca tcacgggcaa accgacggac gaccagacgg aatagcgtcc gcccacccag
    28741 tcgtacatcg caaacacgcg ttccgccgcg ataccgaaag ccgcagctgc cgcagtgtcg
    28801 gcagacaccg cgcaaaaatg gcacgccgtt tcggattccg agaaccccgc accgcgatac
    28861 cacgccttga ctgcctgtgc attgagcagg gtttccggtg ttttgaagga cttgctggca
    28921 acgcaaaaca ctgtcgtttc ggggttcaga cggcataaaa ccgcatccag gcaggcagga
    28981 tcggcgttgg cggcaaaatg gacggcgata tgccgtctga acggctcgag tgcctgaacg
    29041 cacattgccg gcccgaggtc ggatccgcct atgccgatgt ggacaaaatc cgtaatccgt
    29101 ttcccagtta tcccctgata cgaaccgtcg tccaaactgt gtgcaaactc caacgcacga
    29161 tttaactcgc ggcggatttc gggcaacacg tccctgccgt ccacataaac ggcatccgca
    29221 ccgtcgggca ggcgcaaagc cgtatgcagc gcggcacgcc cctcgctgcc gttgactttc
    29281 gcacccgtcc gcaaagcacg cattttccct tccaaatccg ccgtctccgc aagacggcag
    29341 agcagttgca gcgtatcttc gcccaaacgg tttttgctgt aatcgaacaa catcccgtcc
    29401 aaacgctcat gcatacgctc aaaacggtcg ggttcgcagg caaagcggtc gcgcaaaagg
    29461 acatgacgcg tatcctgata atggcgttcg agcgcatacc atgcacgggt aaaagcattc
    29521 atctgttttc cttgattttt cagaaccgga ttaaaatgta gcagaatgta gtttaacaaa
    29581 cggcagcggc tttggcgaat ctccggaaca ccgcccgccg cgtccaatag caccttattt
    29641 ccatatggcg aaaatatagt gaattaacaa aaatcaggac aaggcggcga gccgcagaca
    29701 gtacaaatag tatggaaccg attcacttgg tgcttcagca ccttagagaa tcgttctctc
    29761 ttgagctaag gcgagccaac gctgtaccgg tttttgttaa ttcactatac cctccggccg
    29821 acatttgacc cgcaacaata tcctgcaaga tttattgtgt acgcataaat gccggacagc
    29881 cgcctaaata aggtataatc gcatccgatt ctgtcccgtt tgccgcccgc aggcgggatg
    29941 tcggcggttt tcatcttacc gggaacacgc cggtactgtt ttttaacgat gtttcttaca
    30001 ttttattcca attactttac ggggctagaa tatggctaaa aacggaggat tttctttgtt
    30061 cgcaaagaaa gaaaaacgct ttatctttga aggcaggcat tccgcctccg acaaactggt
    30121 caacggcgaa gtatccgcgt ttaccgaaga agaggcgcgc aaaaaactgg caaaacgcgg
    30181 catccgcccg ttgcagatta cccgtgtgaa aacaagctcc aagcgcaaaa tcacacaaga
    30241 agacatcacc gttttcaccc gccagctgtc cacgatgatt aaagcgggcc tgccgctgat
    30301 gcaggcattt gaaatcgtgg cgcgcggaca cggcaacccg tctatgacgg aaatgctgat
    30361 ggaaatccga ggcgaagtgg aacagggcag ctcgttgagc cgcgcattct caaaccaccc
    30421 gaaatatttc gaccgcttct actgcaacct ggttgcggca ggcgaaacgg gcggcgtatt
    30481 ggaaagcctg ctggacaaat tggcaattta caaagaaaaa acccaggcca tccgcaaaaa
    30541 ggtaaaaacc gcactgacct atccggtatc cgtcatcgcc gtcgccatcg gtttggtatt
    30601 cgtgatgatg attttcgtac tgcccgcctt taaagaagtt tacgccaata tgggcgcgga
    30661 gcttcccgca ctgacccaaa cagtgatgga tatgtccgac tttttcgtct catacggctg
    30721 gatggtgctg atcgcactgg gctttgccat atacggcttc cttaaattga aggcgcgttc
    30781 gattaaaatc caacggcgta tggatgccat actgctgcgt atgccgattt tcggagacat
    30841 tgtccgcaaa gggacgattg cccgctgggg caggacgaca gcgacgctga ttgcggcagg
    30901 cgtgcctttg gtcgatgtat tggactccac tgccggcgcg gcgggcaatt taatctatga
    30961 agaagccacc cgggaaatcc gtacgcgcgt catccaaggt ttgtctatga cttcggggat
    31021 gcgtgcgacg gaactgttcc ccaatatgat gttgcagatg tcttccatcg gcgaggaatc
    31081 gggttctttg gacgatatgc tcaacaaagc cgccgaattt tacgaagacg aggtggacaa
    31141 tgcggtcggc aggctgtccg ctatgatgga gccgatcatt atcgtgattt taggcttggt
    31201 catcggcacg cttctggtcg ccatgtatct gccgctgttt aatttgggca acgtggtcgc
    31261 ctgatttgcc gcacagatcc ggcgcggatt cgctctgcgc cggtttgttt ttgttttgaa
    31321 tacattgaga acaaaatatg tctgatttgt ctgtattgtc gccgtttgcc gtgcctttgg
    31381 cagcggtgtt cgggctgctg gtcggcagtt tcttaaacgt cgtcatttac cgcgtgccgg
    31441 tcatgatgga acgcggctgg acggtatttg ccaaaaaaca tttaaacctg ccgctgaccg
    31501 aaaaggaaag ccgtaccttc aacctgatga aaccggattc ctgctgtccc aaatgccgcg
    31561 tgccgatacg cgcgtggcag aacatcccga ttgtcagcta cctgctcctg cgcggcaaat
    31621 gcgcttcctg ccaaaccaaa atcagcatac gttatccctt aatcgagctg ctgaccggcg
    31681 tattattcgg gctggtcgcc tggcaatacg gctggtcttg gattacattg ggcggtttga
    31741 tactgaccgc gtttctgatt tccctgacct ttatcgatgc ggacacccaa tacctgcccg
    31801 actcgatgac attacccttg atttggctgg ggctgatatt taatttggac ggcggcttcg
    31861 tgcctttgca gtctgccgtt ttaggtgcgg ttgccggcta tggttcgata tggcttttat
    31921 gcgcggtata taaactgatt aaaggcgaaa tcggtatggg caacggagat ttcaaactga
    31981 ttgccgcatt gggcgcgtgg ctcggcatat ccgcattgcc cgtgctgatt tttgtttcct
    32041 cgctgatcgg tttggtcgcg gcaatcgtta tgcgcgtcgc caaggggcag cattttgcct
    32101 tcggccccgc actgacagtt tcgggctgga taatttttac ggcaaacgat tccgtatggc
    32161 gggcggtcaa ctggtggctg acccatccgg tgctgtaaga tgacggtatg ggtcggactg
    32221 accggcggaa tcggcagcgg caaatcggca gccgcgcaat gttttgccga tttgggcgtg
    32281 ccgcgcatcg atgcagacgc ggcggcgcac tcgctgacgg cttcagacgg catcgccctg
    32341 ccggaaatca ggcggctgtt cggcgacacc gttttcgaca cacagggttt gttgcggcgc
    32401 gacatattgc gtaaagaagt ctttgcctcc ccatcgcgaa aagccttgct cgaatccgtg
    32461 atgttgccgc tgattttctc agaaatcaaa aaacagcaag aaacctttac cgatgcggct
    32521 tacggcattg tcgaaattcc gctgctgacg gaaaagcgtc aatttatcat cctgatacgg
    32581 cgtgtcctga ccataagtgc ccctgtggaa aaacgtatcg gcagggtgat ggcccgcagc
    32641 gggctgacgc gcggcgaggt ggctgccgtc atcagccatc aggcatccga atccgaacgc
    32701 ctgctgcttg cagacgatgt gctgctcaat gacggcagcc tcaaaagcct gcgtgagaaa
    32761 acaatgcgcc tgcacgcgtt ttattcaggg attttcgcct caaaaccaac acaaggaaaa
    32821 cacaatggct gaatcgcggc aaacacgcct tcaagtcaaa tgtccgacct gtcaaacagc
    32881 agtagtatgg gaacccgaaa acgcattccg ccccttctgt tcgcaacgct gcaaactgat
    32941 cgacttgggc ggatgggcag acgggaaata tacggtttcc ggccaaacgg aaagtttgcc
    33001 ggaaatatcc gaacccgaca tggcataccg ctgaccgccc cgccttcccg gcaaacaccc
    33061 tgaaagtcaa atgccgtctg aaacaaacac gcttcagacg gcattttcat tctcaaacct
    33121 aatcgttggt atttgccgtt acctcttcca atgaagtaat gccctgcata actttcaaaa
    33181 taccggcccg gcgcaaatcc accataccct ccttataggc aacgtccaaa atatccactt
    33241 ccgtaccgtt gttcataatc acacgctgca tttcttcgct gatgggcata acctcataca
    33301 cgcccgcacg ccccttataa ccctgccccc ggcaacggtc gcaaccgacg gcgcggtaaa
    33361 gtttccaatc ttttgcaagg tcctcatcgg tgaagccgac ttccttcaaa gcagaggcag
    33421 acgggcgttc cacttcctgt ttgcagctcg aacacagcct gcgtaaaaga cgctgcgcca
    33481 taatcaggct gaccgaactg gcaatattaa acggcgcgac acccatattc agcatacgcg
    33541 acaacgtcgc cggcgcatta ttggtgtgca gggtggaaaa caccatatgc cctgtttgtg
    33601 ccgccttaat cgcaatatcg gcagtttcca aatcacgaat ctcaccgacc ataatgatgt
    33661 ccgggtcctg acgcaggaaa gacttcaaag cagcggcaaa agtcaggccc tgcttatcat
    33721 tgacgttaac ctgattgatg cccggcaggt taatctcggc agggtcttcc gccgttgcaa
    33781 tatttaccga ctccgtattc aaaatattca aacaggtata gagcgacacc gtcttacccg
    33841 aacccgtcgg accggttacc agcaccatcc cgtagggacg gtgaatcgct tccaacaaca
    33901 attttttctg aaacggctca aaaccgagct ggtcgatgtt caaagacgcg gcatcggaat
    33961 tcaaaatccg catcacgacc ttttcgccaa acagcgtcgg caatgtgctg acacggaaat
    34021 cgacaggctt gccgcccttt tgaaaggtca gctgcatcct gccgtcctgc ggtatccgtt
    34081 tttcggaaat gtccaaacgc gacattacct taatccggga agcaagctgc ccccttaccg
    34141 caatgggcgg ctgaaccacc tcgcggagct gcccgtccac acggaaacgg atacgggcat
    34201 tgtgttcgta aaactcgaaa tggatgtccg atgccccgct gcgcaaggca tccgacaaag
    34261 tcttatggat aaacctcgga acagggccat cttctgcctc ctcgttgtcg atatacaggg
    34321 tgtggctttc ctcttcctcc tgcccctccc caagctcctg aagcagcgat gtcgaacgcg
    34381 aacccaccca atcgagcaaa cccgccaact ggtcatcctc gacaatgacc aactcaacct
    34441 caatccctgc ggcagaaacg gttttctgaa tttgcggcat ctgtgtcgga tcggaaaccg
    34501 caaaaaatac tttgtcgccc cgacggaaaa ccggcacaca gtggaactcc accatctgct
    34561 cctccgtcaa cacccccatc agcaccctgt ggcgcggata atgacgcaaa tcaagaatcg
    34621 aataactgaa caccctcgca atcaatgccg caagcgactt gggcgaaatg acaccgtctg
    34681 aaaacagcat cggcaacact tccttacccg cctgcgactc attgtagtaa tgctcggcct
    34741 gctcaacagt aaccacctgg ttttgaacca gaatcctcag caaacctacg ctcatacgac
    34801 cttatcccca aatttattca ttgttatacc tgtacag
//
LOCUS       DAXHPG010000004        29752 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXHPG010000004 DAXHPG010000000
VERSION     DAXHPG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29752
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.004"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(213..860)
                     /locus_tag="ACFM6F_000127"
     CDS             complement(213..860)
                     /locus_tag="ACFM6F_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC6163628.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(857..1225)
                     /locus_tag="ACFM6F_000128"
     CDS             complement(857..1225)
                     /locus_tag="ACFM6F_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC6163629.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(1235..1906)
                     /locus_tag="ACFM6F_000129"
     CDS             complement(1235..1906)
                     /locus_tag="ACFM6F_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC6163630.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(2071..2790)
                     /locus_tag="ACFM6F_000130"
     CDS             complement(2071..2790)
                     /locus_tag="ACFM6F_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6163631.1"
                     /translation="MATLSNKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            complement(2874..4187)
                     /gene="hpnE"
                     /locus_tag="ACFM6F_000131"
     CDS             complement(2874..4187)
                     /gene="hpnE"
                     /locus_tag="ACFM6F_000131"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC6163632.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALADLQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            complement(4266..5138)
                     /gene="hpnD"
                     /locus_tag="ACFM6F_000132"
     CDS             complement(4266..5138)
                     /gene="hpnD"
                     /locus_tag="ACFM6F_000132"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC6163633.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTILYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDSKTLEYADKMGLALQLT
                     NIIRDVGEDARNGRIYLPMEEMRRFDVPASVILQCRPTENFTELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5309..7171
                     /gene="hscA"
                     /locus_tag="ACFM6F_000133"
     CDS             5309..7171
                     /gene="hscA"
                     /locus_tag="ACFM6F_000133"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC6163634.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVKQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVHAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7248..7904
                     /locus_tag="ACFM6F_000134"
     CDS             7248..7904
                     /locus_tag="ACFM6F_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6163635.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISEVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAESGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYFRKNGRRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAESRKPR
                     "
     gene            7940..8701
                     /locus_tag="ACFM6F_000135"
     CDS             7940..8701
                     /locus_tag="ACFM6F_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC6163636.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFEYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            8756..9097
                     /gene="fdx"
                     /locus_tag="ACFM6F_000136"
     CDS             8756..9097
                     /gene="fdx"
                     /locus_tag="ACFM6F_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC6163637.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9384..9971)
                     /locus_tag="ACFM6F_000137"
     CDS             complement(9384..9971)
                     /locus_tag="ACFM6F_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFC6163638.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSGGGFT
                     VTAESVSAACPELEGRYLELVRRTATSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            10184..10381
                     /gene="iscX"
                     /locus_tag="ACFM6F_000138"
     CDS             10184..10381
                     /gene="iscX"
                     /locus_tag="ACFM6F_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC6163639.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10449..10850)
                     /locus_tag="ACFM6F_000139"
     CDS             complement(10449..10850)
                     /locus_tag="ACFM6F_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6163640.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            11061..12020
                     /locus_tag="ACFM6F_000140"
     CDS             11061..12020
                     /locus_tag="ACFM6F_000140"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC6163641.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            12088..13398
                     /gene="tilS"
                     /locus_tag="ACFM6F_000141"
     CDS             12088..13398
                     /gene="tilS"
                     /locus_tag="ACFM6F_000141"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC6163642.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRYFLKENGIPMP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            13472..14257
                     /locus_tag="ACFM6F_000142"
     CDS             13472..14257
                     /locus_tag="ACFM6F_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC6163643.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEGKMPSEEVRRLRAVVPEGKVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLSGIYPQADLEIWLARYKGRVFATALREEKQAVLYGEDLCGPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            14267..14464
                     /locus_tag="ACFM6F_000143"
     CDS             14267..14464
                     /locus_tag="ACFM6F_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163644.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            14726..15079
                     /locus_tag="ACFM6F_000144"
     CDS             14726..15079
                     /locus_tag="ACFM6F_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163645.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            15091..15396
                     /locus_tag="ACFM6F_000145"
     CDS             15091..15396
                     /locus_tag="ACFM6F_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163646.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKGNAQKDGSDAGIRPH"
     gene            15458..16834
                     /gene="mpl"
                     /locus_tag="ACFM6F_000146"
     CDS             15458..16834
                     /gene="mpl"
                     /locus_tag="ACFM6F_000146"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC6163647.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTVVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVGIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     GKLLDALR"
     gene            17041..18093
                     /gene="bioB"
                     /locus_tag="ACFM6F_000147"
     CDS             17041..18093
                     /gene="bioB"
                     /locus_tag="ACFM6F_000147"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC6163648.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(18171..18806)
                     /locus_tag="ACFM6F_000148"
     CDS             complement(18171..18806)
                     /locus_tag="ACFM6F_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6163649.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDYAKV
                     RKLITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQRPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQVGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            complement(18930..19169)
                     /locus_tag="ACFM6F_000149"
     CDS             complement(18930..19169)
                     /locus_tag="ACFM6F_000149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163650.1"
                     /translation="MGVCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVRRILSVHIAGI"
     gene            19156..19389
                     /locus_tag="ACFM6F_000150"
     CDS             19156..19389
                     /locus_tag="ACFM6F_000150"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6163651.1"
                     /translation="MQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHSNDFGAQGIT
                     ATAAGGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(19452..21311)
                     /gene="ilvD"
                     /locus_tag="ACFM6F_000151"
     CDS             complement(19452..21311)
                     /gene="ilvD"
                     /locus_tag="ACFM6F_000151"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC6163652.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(21484..23253)
                     /gene="cysI"
                     /locus_tag="ACFM6F_000152"
     CDS             complement(21484..23253)
                     /gene="cysI"
                     /locus_tag="ACFM6F_000152"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFC6163653.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGGGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIDELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVE
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(23280..25094)
                     /locus_tag="ACFM6F_000153"
     CDS             complement(23280..25094)
                     /locus_tag="ACFM6F_000153"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HFC6163654.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSVLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVRRAELKD
                     YKAKNISGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFTASADGWTGRIVARLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARVGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(25091..26377)
                     /locus_tag="ACFM6F_000154"
     CDS             complement(25091..26377)
                     /locus_tag="ACFM6F_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HFC6163655.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLDCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAGRLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAKVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(26416..27339)
                     /gene="cysD"
                     /locus_tag="ACFM6F_000155"
     CDS             complement(26416..27339)
                     /gene="cysD"
                     /locus_tag="ACFM6F_000155"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFC6163656.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(27377..28117)
                     /locus_tag="ACFM6F_000156"
     CDS             complement(27377..28117)
                     /locus_tag="ACFM6F_000156"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HFC6163657.1"
                     /translation="METTLLKPALWQIPHIGSGSETALAEKTETLKQRLHRIAGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(28128..29579)
                     /gene="cysG"
                     /locus_tag="ACFM6F_000157"
     CDS             complement(28128..29579)
                     /gene="cysG"
                     /locus_tag="ACFM6F_000157"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFC6163658.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKDKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGD
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQAYPALGGLNAGQRAA"
BASE COUNT         6360 a         7855 c         8607 g         6930 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta taccatacaa ccacgccgga attaagttta
       61 aatttgaata aaaggttcgg gttctgcaaa atacagaacc cgaaccttgt tcggatattg
      121 aaaccggccg cccgattttg ggcggtgcgg cttgcaagta tcaagattcg catatgccgt
      181 ctgaagctcg gagaggttca gacggcatat gcttatttgg gctgctcttc gacgaatctc
      241 ggacctttca agatgccgtt gtgagaatag ggcgacagca ggttgtatgc ggcggttttg
      301 gaaacctgat aaccgcggtc ggtcaggctg ttggcaatct gattgaccac tgcgctgacc
      361 aaagccccca acaggccgct gttgctgttg ttgctgcctt cgcggatgct ggccgaaccc
      421 gaccacaact cttttccgtt gcgggaatcg accagccgtg ctttggcgga tacggtcgtc
      481 acgctgtcta aaatttgata tgaagtgccg tattcggtaa ccgtaatgta caaaaccgca
      541 tcattgccga aaatctgatg cagtttttcc ggccggacgg cgtgaatatc ggcggcattg
      601 gtcaagccgt tttgtttgaa ggtttcctcc acgactgcgg cggggaagac gtaatagccg
      661 gcttcggaaa gcggcgcggc ggtcgaagcc agtacacccc atgttccgtt gacatcgggc
      721 gattcgttca gcggcggaac caccaaaatt gaagccggtt tgctttcctt gaatgacgtg
      781 tagtcgaaat cgggcgcttt ttgaacttgg caggcagaca gcgccaacac ggcggcaagc
      841 cctaaaatca aaggtttcat cgcttgcctc ctttaccggt tttcatcagg aagtccataa
      901 atacgcccga ttcgggaaac agccttttct cttcttcaaa ctggcggaac gcgccctctt
      961 tgtctcccga acgggaaagc agcagtccca gatgggcgtg cgcacccggg gcggcattca
     1021 tttttttgtt gccggcttcc acaaagtatt tttccatctt ttcggtctgc ttgcccaacg
     1081 aagtgtcgtc gtttttcaaa ccttcataga cggtatcggg atagccgccg taataataca
     1141 gggatttttg cccgttgccg ccgcaggcgg tcagagccaa gaccgccgca cacagcgaca
     1201 aacggctcaa ggttttcgga ttcatcattt ctccttaacg gttgggttgc catgcgccgt
     1261 tgtcaacagc ctgaaccagg ctgttgacgg cttcgcggat tgccaagtct aaaactttgc
     1321 cgttcaaagt cgcatcgtag ccggaagtgc cgccgaaacc gatgatttca cggttggaaa
     1381 gtgcgtattc gcccgcgccc tgtgcggaat agacgatttc ggaagtattg acgttgacga
     1441 tattcagagc cacttttgca taggcgattt gcgatttgcc gcgacccaaa atgccgaaga
     1501 gctgatgatc gccgacatct ctgcgtccga attcggttac atcgccggta acgacataat
     1561 ctgcgccttt caggttatgc gctttgccgg aaatgccgga ttcctgtttt aatgcgttca
     1621 aattggtgcg gttcagtacg ttgaagcggt tggtctgttg caggtgcgtt actagaatgg
     1681 tttttgcctg gctgcccaaa cggtcttccc cgtcggagaa aatgcctttt tggaagctgg
     1741 agcggttgtc gaatgttccg acggaaatcg gggtacgaac accgtgatat tgcgtattgt
     1801 aggaggcgac tttctctacc tcgagactgc gtgaggattc ggtcgcacag ccggtcagtg
     1861 aaacggcagc ggcggcaagg acaacggcgg tggaaacggt tttcataaaa tttaccctaa
     1921 ggtcaagtta aggaaataac gggttatcat tattgtcctt atgtaaattt aagtcaaggt
     1981 gtttgtctgt gcgggacgga tgcgcgcgga aggtacggat atttttcaaa cggaatcgcg
     2041 gcgcgggcgg gatgcctgcc ttttccggat ttagaggtaa acgatttcgc cgcttcgccc
     2101 tttgctttcg gtacttgccc accagacaaa tgcgggcagc acgtccccgt agcttttgcg
     2161 ttcgcttttg gcttcgcccg gatgggattt gatgcgttgc ggggaattga tgggtccggg
     2221 gacgaggacg ttggcgcgca ggttgccgaa gcgttcccat tcgtcggcgg cgactttgca
     2281 caggtagttc aacgcggctt tggacgcgcc gaagccgccc cagtaggctt tgggtgtttc
     2341 gccgtggctt tcgccgacga agatgacgga cgcgtcgggc gactgcttca gcagcgggaa
     2401 cagggcgcgg gtcagcccca taggtgcgac ggtgttgatg cggtattggt tgacccattc
     2461 ggcgacggtt tggaaatcca gcggcgagag ggcgtaaaaa tagccggcgc agtggacgat
     2521 gccgtccagt ttgccttgcg tggcttcggc aatggtggcg gcgaactgat tgaattcttt
     2581 ttcttccgca ctcatcaggt cgaaacggat ggcgaagggt tcggggtgtc cggcttcgac
     2641 aatcgcgtca tagacttttt ccagtttttt ctgatggcgt gccaccagaa tcacggttgc
     2701 gcctgccgcc gcataggctt tggcgacctg ttcgcccaga ccttgcgatg cgccggtaac
     2761 taagatggtt ttgttggaca gtgtcgccat actttttcct ttttggtggt cggtcggggg
     2821 tattttagcg ttttgccgca ccttgtaaag cgtcccgata ccagccggcg ttttcagacg
     2881 gcatcgctca ggctttgcag gcaggtttcc gccgccgcga aacctgattg tacggcggct
     2941 tcgagcgtgg cggggtagtc tgggtggagg tagtcgccgg cggggaagat gcggtgccgg
     3001 tgcagccacg acaaatccgg cggcggggca tcggctgcgg ttgtggcgcg tttttcggtg
     3061 atgacgcgca cggcttcggg ttcgcccaaa tgcggaagga tgcgtttgag gtcggcgtga
     3121 actttgtccg cccacgctcg gtttgtaaac gcgccgacgc ggtcggaaac gctgatgacg
     3181 gcggacactt cgttttcagg cagtccgagc ctgccccggc aaagcagcca ttgcaccgtg
     3241 ccgtcggcaa ggccggtcag cggggcgggc aggcggacgg gttcggcgta gcgcagatag
     3301 acggtggtga tggcgtggta gcgaaggttt tgatatgccg tctgaacgtg ttcgggcgtg
     3361 ccttcgggca ggagcgcggc ggcgtggtag ggcgcggtgg cggggacggc ggcatcgaaa
     3421 gcttcgccgt tgacgagcac tttcccgtcc gggagggtgt tcagacggca tacgcgcgtt
     3481 tcgaggcgga tgtccgcgcc gagccgttga agatccgcca aggcgggttc ggcgacgatt
     3541 gcgcccaaat cctgcttggg taggagatag tcgctgccgg attttttcgt cagcacgccg
     3601 tcggacaaaa cgttgcacaa cacgcgcagg cttgcggttt ccaaaggcgt gttgagcgcg
     3661 ccccaaacca agggctgcca aaactgcatc acggcggcac gcggcacgtt ccgctgtttc
     3721 agccattgcg ccactgtcgt gtcgggctgt ccgaggcgtg cggacttctg caaatcggac
     3781 atatcggcaa gcagtttggc tttgaatgca gtcggtgcac gccgggcaag cagcacgccg
     3841 cccaaaatat gcagcggcgc gggcaggggg agggcgcgga actgcaaacc gccgtgcata
     3901 tgccagtgca gcggtacgcg caaaaaggcg gcacggggat ccgaaccgat ggttttcatc
     3961 aggcgcaaca cgccccggta tgcgccgagc aaaatgtgct gcccgttgtc caaaaaaccg
     4021 aaaccgtcgg tatttccggc cagtgtgcgc gccctgccgc ccgcctgccg gccggcttca
     4081 aacagggtaa cgtcggcgtg ccgcgccaag gtgacggcgg cggacagtcc tgcccagcct
     4141 gcgccgatga cggcgatttt cgggcgcgga tgcggcgtgt tcatcattta ttcctccaat
     4201 ggttgaaacc ccgtttttcg gggcggcggg ggttgggttt tcctgaaggg cgggcggtat
     4261 gcgccttatg cccgttccgg cgtgccgggg cgcggtttga atccgaataa ccaggttttc
     4321 agggcaatgc gttttttgcg cggcgaaggg agggcgattt tgtatttgag gacgttttgc
     4381 gcgccgtctc ggtcgatttc gtttaaaagc gcgtaataaa ccgccgccat aaccagtccg
     4441 actttttggg cttttttgtc ggcatcaggc agcagcgata cggcttcacg gtaggtttca
     4501 cgcgcacgct ttatttggaa tgccatcaat tcggtgaaat tttctgtcgg ccggcactgc
     4561 aaaatcacac ttgcggggac atcaaaccgc cgcatttcct ccatcggcag gtaaatccgc
     4621 ccattacggg cgtcttcgcc gacatcgcgg atgatgttgg tcagttgcag cgcaagtccc
     4681 attttgtcgg cgtattccag tgttttgctg tctgaaaacc ccaaaatccg cgcaatcagg
     4741 cagccgacta cgcctgcgac gcggtggcaa tacagtttca attcctcaaa actgccgtaa
     4801 cgcgcctgaa ccaaatccat ctgcatcccg tcgattaagg cttccagttc atatttcggc
     4861 agcttgaagg tttccttaac ttgccgcaag gcctgattga cggggtgttc cggcatcgcg
     4921 ccgccgaata ccttgtccaa atcgccgcgc caccagttca atgttgcctg tgcaacatcg
     4981 gggttggaac attcgtcaac cacatcgtcc aattcgcggc aaaaggcata taaaatcgtt
     5041 accgcatccc gtttttcctg agtcaggaaa cggaagcccg ataaaaaact ggagcggctt
     5101 tcttctgctt tttgacggca atagtcgagt cctttcacga tttatattcc taatgatggg
     5161 cgggaaaggc ggattttatc ggcatttggc ggtagagggc aatttcggcg gcacgaccca
     5221 atccttagcg gtttgctcaa ctatcggcgc gaattctgtt aaaatgccgc tttccttcct
     5281 ttacacaccg caccgacagg cagaatttat ggctcttttg cagatttcag aaccgggtat
     5341 gtccgccgcc ccgcaccggc accgtttggc agcaggcatc gatttgggta cgaccaacag
     5401 cttggttgcc accgtccgca gcggcagtgc cgcctgcctg cccgatgccg aagggcgcgt
     5461 taccctgcct tccgtcgtcc gctatctgga aaacggcggc attgaagtcg gcaaaaccgc
     5521 cctgtccgcc caaaaaaccg acccgctgaa caccgtcagc tccgccaaac gccttatcgg
     5581 gcggacgctt gccgatctgc atcaaaatac gcactacctg ccttaccgtt tcggcgacaa
     5641 tcaacgcgtt atcgaattgc atacgcggca gggggtgaaa acgcctgtcg aagtgtcggc
     5701 ggaaatcctc aaaaccctta aatcgcgcgc cgaagaaacc ttgggcggcg atttggtcgg
     5761 cgtggtgatt accgtccccg cctatttcga cgatgcccaa cgccaggcca ccaaagatgc
     5821 cgcgcggctg gcgggtttga acgtattgcg cctgctcaac gaacccaccg ccgccgcaat
     5881 cgcctacggg ctggacaacg cctcggaagg cacgtttgtc gtgtacgact tagggggcgg
     5941 cacattcgac gtatccgtat tgcaactgac caaaggactg tttgaagtca aagccaccgg
     6001 cggcaacagc gcgttgggcg gcgatgactt cgaccatcgt ttgttctgct acctgctcga
     6061 acaaaacgga ctctcccaac tcaacgaaca agacagccaa ctcctgcttt cgctcgtccg
     6121 cgccgccaaa gaacaattaa ccacgcaaac cgaagcgcgc attcaggcga cgctttcaga
     6181 cggcatggca atcgacacaa gcatcagtcg cgccgagttc cacaacctga cgcagcattt
     6241 ggtgatgaaa acgctcgaac cggtcaaaca ggcgttgaaa gatgccggtg tcggtaaaaa
     6301 cgaagtcaaa ggcgtaatta tggtcggcgg ttcgacccgt atgctgcacg tccagcaggc
     6361 agtcgccacc tttttcgggc aaaccccgct gaacaacctc aaccccgacg aagtcgtcgc
     6421 gctcggcgca gccatacagg cgaacgtcct cgcaggcaac aaagccgacg gcgaatggct
     6481 gctgctggac gttacgccct tgtcgctcgg tttggaaacc tatggcggct tggcggaaaa
     6541 aatcatcccg cgcaattcca ccatccccac cgcgcgcgcg caggacttta ccaccttcaa
     6601 agacggtcag accgcgatga cgatacacgt cgtacaaggc gaacgcgaac tggttgccga
     6661 ctgccgcagc cttgccaaat tcaccctgcg cggcattccg ccgatggcgg cgggtgcggc
     6721 gcgtatccgc gtaaccttcc aaatcgatgc ggacgggctg ctgtccgttt ccgcccaaga
     6781 acaaagtacc ggcgtacacg cgcaaatcga agtcaaacct tcctacggtt tggatgacgg
     6841 cgcgattacc cgaatgctta aagacagtat ggacaacgcc gccgaagata tggcggcacg
     6901 cgcccgcgcc gaagccgtgg tcgaagccga aagcctgacc gatgccgtca acgccgccct
     6961 cgagttggac agcgatttgc tggatgccga agaattgcaa cagattcggc aaggtatcgc
     7021 cgatttgcaa ggccgtctga aagacggaaa agccgaagac atccgcgccg ccgccgccaa
     7081 actcggcagc atcaccgaca atttcgccgc caaacgcatg aaccgcaaca tccaacgtgc
     7141 gctgacaggc cagagtgtcg ataatatttg atacttaaac ggtttcagac ggcatagaga
     7201 taacctgatg ccgtctgaag gctcgaaaac acttgaaaaa catcgatatg gaaaagtcag
     7261 gcattgtcta tctgatgaaa accgtcatca agggcgtata taaaatcggc atttcggagg
     7321 taagcaattt tgaaggcaga atgcgccatt tggaaaacaa cggctatgcg aacgttgccg
     7381 gattggagcg catcctcgcc gtcaaaaccg acaattacaa agaaaaagaa aacctgctcc
     7441 atgaaatttt cagcaaaagc aggataggcg ataccgaatt gttcgccgtg gacgaaaacc
     7501 ttgtaaaacg tttgttttta tcgcttcgcg gcgaaatcgt gttcccgaaa aacgaaacgg
     7561 cggaatcgga atttgaaaaa agcgtccacg aacgcaggca ggaagggaat gccgaatccg
     7621 gccgcaaaca actgcttgat ttggtacggc gcggacaccg ggaataccct tacgcgctgc
     7681 cccggctttt ggcgggcgcg gcatcctaca agccgaaaaa atcgaaaatc cgccttttta
     7741 aagaagcata tttccgcaaa aacggcagga ggctgacgga cgaaattgca gacggcatcc
     7801 atatttacac ctgtttttcg cgggcggatt tggaaaaagc ctattccgaa tatttggaac
     7861 ttttcaaatc cgaatcggat gccgaaagca gaaaaccacg gtaaggtgca aacagatacc
     7921 gtacacgttg aggagcagta tgatgggcga ttccgtcatt tattatgtag aacaggcaga
     7981 caaaccggta aaccgtgccg gcgaacgcgc ccgtaaaaca ttcgaatatt tttggcgcga
     8041 gcttttttgg gaacgccgcc gcatcattcc cgccttagat tttgccatgg tcaaagcccc
     8101 ttttttccaa gacggcgaag acggcgaaat ttgcgaacat atgtggatcg acgatattta
     8161 ttttgacggt ctttacattt atggcgtgct gaacaatgaa cccggcgaac tgaccaatgt
     8221 cgaacaaggc gaaagcgttt gcgttccggt tgacgacatc agcgactgga tgttcgtgtg
     8281 caacggcatt ccctacggcg gctttaccat acaggcaatg cgtggacaga tgacggaaga
     8341 ggagcgtacc gaacacgatg ccgcatgggg aatcgatttc ggcgatcccg ggcaggtatt
     8401 gctggtgtat gaagaaaaag aacatcccga aaatctggaa gagcatccga tgtgccggaa
     8461 ctgtattgac gattttcggc aacagttgtc ccaaaatccg gattttctgc atgagcagga
     8521 cgaagacggc tatacgccgc ttcatcatga agccatcgca ggaaatgcac ttatggttca
     8581 agccatgctt gaatacggcg caaatcctgc ctcaaagaca tcggaaggct ataccgccct
     8641 cgattttgcc cgcctggcgg gctggcaaaa tgttgccgac ctgctcgaac cgcgacatta
     8701 ggcggacagt tttccgaaaa cgaacacaaa cactttttac agaaagacaa taaaaatgcc
     8761 caaaatcacc gtacttccac acacgacatt atgccccgaa ggcgcggtta tcgaagacgc
     8821 gcccgaaggc aaaaccgtcc ttgacgtgct gctcgaccat gatatcgaag tcgatcacgc
     8881 ctgcgaaaaa tcctgcgcct gcacaacctg ccacgtgatt atccgcaaag gtttcgacag
     8941 cctggaagag ccgaccgaat tggaagaaga cctgctcgat caggcttggg gtttggaagc
     9001 cgattcgcgc ctgagttgtc aggcggttgt cgccggcgag gatttgattg tggaaatccc
     9061 caaatacacc atcaaccacg cgcgagaaga acactgaaaa caggccgcct gaagccggca
     9121 cgcttcagac ggcattgttg cgcggataag gcgcaatcgc ccgaaaacag gcgttcgtac
     9181 aggcggaact ttcgattcta tagtgaatta acaaaaatca ggacaaggcg gcgagccgca
     9241 gacagtacca atagtacggc aaggcgaggc aacgctgtac cggtttaaat ttaattcact
     9301 atatattgat ttttatcggt tttctgacgg aacggattgg tgcggcatcc gaggcggatt
     9361 actcgggcgc gatgcacggg tatttatcgg ttttgcagcc ggaaaaaccg ccggcgggtt
     9421 actttcaggg cggcggtcgg gcggtatgcc ggatagcgtt ccactttcgc ttctatattg
     9481 attttgattg gttttctgac ggaatgatcc gatgcggcat ccgggacggc ttgtgtttct
     9541 tcctgcccgc ctgccggata ttcctgtact tgcacgaaac cgaaagaggt ggcggtgcgg
     9601 cggacaagct cgagatagcg tccttcaagc tccggacagg cggcggacac gctttctgcc
     9661 gtaaccgtga agccgccgcc cgacccggca aggcgttccg caatctgcgt gtagatgcgc
     9721 caacgttggg cgcgcaacat tgccgcatcg caaccggcag ccgctttatc caaggcaatc
     9781 agaccgcgcc tgctccggta gtgaaggcaa accgttactc tggagaggat atgttgcccg
     9841 tccaatattt gatacagttt gcgtatcagc agaatcaggc ggtcaaactc ctccatctcc
     9901 gacagggaat caggatggtc ggaagcggta taaaccagtt tggacaattt tgcggcaaac
     9961 atattgttca tcaatcttcc ttgtcggttg aaaccccgct cttcggggcg gtagaatcag
    10021 atttgtttgg gagggacaac tcctcccaga tcaggacgac acataggctg gtgcttgatg
    10081 tgttgtccgg cgagttgaaa cattcagcca tcctcaaggt gcggcagttt tgccgaaaca
    10141 tattctacac ggcttcaatg ccggacgata aaaggaaatt catatgaaat ggaccgacac
    10201 ccggcgtatc gccgaagaac tctatgacct gcacggcgaa accatcgatc ccagaaccgt
    10261 gcgctttacc caactgcgcg acctgattat ggcattgccc gaatttgatg acgatcccgc
    10321 ccgttgcggc gaacgtattc tcgaagccgt gcagcaggca tggatagacg aggcagaata
    10381 agtttcggga atgccgtctg aaatacggcg gtacgcggtt cgtgcttctg tttgcagtgg
    10441 gaacggtttt accagtctcc ttttttcagc ctatccagtt ggcggcggtc gcgcttggtc
    10501 ggtctgccgt cgggatgggc ggaagtgatg cggctgaatt ggtcgagctg tttgcgctct
    10561 tccctcaatg ctgccgtttt tgcgtcttct tcatacagaa gccgcgcctc ggatgccggg
    10621 cggcgttggt ggttcaaacc tttaaccttg attttatagg gaagggaatt gagcgtcagg
    10681 tcgataatat cgccgatgtc tatggtttta ctgtttttga ccttcgagcc gtttacttga
    10741 accctaccca gttcgatgtg cttttgcgca agggaacggg tcttgaaaaa acgtgccgcc
    10801 caaagccatt tgtccagccg catggcggaa gaatcgtgct tgtctttcat acgattttgt
    10861 ttgaaataat tgaatttgtt tcgagtttag cataagatac gccgcctata actagtatat
    10921 atgcactaat ccactgtttt ccatgctgtc cgaacacaaa aagagggtgt ggaaaagccg
    10981 ttttggacaa taaattaact gcggaatatg cacaaatagc gtatgatagc ggcagaatct
    11041 gttgatgaga gcttcattct atgaaacctg tttttttgga ttttgaacaa cccatagccg
    11101 aactgaccaa caaaatcgat gagctgcgtt tcgtccaaga cgagtctgcc gtcgatattt
    11161 cggacgaaat acaccgtttg cagaaaaaaa gcaacgacct gaccaaatcg attttcagca
    11221 aactcacacc cgcccaaatt tcacaggttt cccggcatcc gcagcgtccc tatactttgg
    11281 attacattga ggcactgttt accgattttg aagaactgca cggcgaccgc cactttgccg
    11341 acgattatgc gattgtcggc ggattggcgc gtttcaacgg acaaagcgtg atggtcgtcg
    11401 ggcatcagaa agggcgcgac accaaagaaa aaatccgccg caacttcggt atgccccgtc
    11461 ctgaaggcta ccgcaaagcc ctgcgcctga tgaagacggc agaaaaattc ggcttgcccg
    11521 tcatgacctt tatcgatacg ccgggcgcgt atcccggcat cggcgcggaa gaacgcggac
    11581 agtcggaagc catcggcaaa aacctgtacg aactgacgcg cctgcacgtc cccgttttgt
    11641 gtaccgtcat cggcgaaggg ggctcgggcg gtgcgttggc ggtcgccgta ggcgattacg
    11701 tcaatatgct gcaatattcg acctattctg ttatctcccc cgaaggctgc gcttcgattt
    11761 tgtggaaaac cgccgaaaag gcggcggatg cggctcaggc tttgggcatt actgctgacc
    11821 gcctgcaaaa gctggacttg gtcgataccg tcatcaaaga accattgggc ggcgcgcatc
    11881 gggatttcgg gcaaaccatg aaaaacgtaa aagccgtttt ggaaaaacaa ctgcacgaag
    11941 cgcaaagcat cccgcttgcc gatttgcttt cgcgccgttt cgaccgcatt atggcttacg
    12001 gcaaattttc ggaacaataa ttcaggtaga acaagcagca agcagtttgt ctgaaactgc
    12061 ttgctttttc tttatcggga cggaaccgtg ctgactttag atgcgtttga gcaatgcttg
    12121 aaggattgtt ttcctcaagg tctgaatgga aaaaaaacag cggtggcatt aagcggcggc
    12181 ttggattccg tcgttttgct gcatctgctt gtccgcgccg ggaaaaaagg cggttttatt
    12241 ccggatgcat tgcatatcca tcacggcttg agtccccgtg ccgacgattg ggcggatttc
    12301 tgccaaaact attgcgatat gctcggggtg gggctggaaa cggttaagat ctgcgtggaa
    12361 aaaaacggtt tgggcatcga ggcggcggcg cggcaaaagc gttatgccgc gtttgccgaa
    12421 aaaggctttg acgttttggc gttggcgcac cacagggacg atcaaatcga aacctttatg
    12481 ctggcggtcg cgcgcggcgg cggtttgcgc gctttggcgg ctatgcccgc cgtccgccct
    12541 tttggggaaa aaggcattat ctggcggccc ttgctgcctt tttcgcgcca agatatatgg
    12601 gattatgccc aaaaacacgg tttgccgaat atcgaggacg aaagcaatag cgatacggct
    12661 tatttgcgaa accgcttccg gcaccgtatt ttgcccgaac tttcggcgca gattccccat
    12721 ttcgggcggc atctgttgaa caatgtccgt gccttgcagg aggatttggc tttgttggac
    12781 gaggtcgtcg ttcaagactg ccgttgggtt tgcggagccg gttatttcga tacggcgcgg
    12841 tggctgacgt tttccccgcg ccggaaaacc catattttgc ggtatttttt gaaggaaaac
    12901 ggcatcccca tgccgaatca gaatgccctt gccgacattg cccgggtttt gacggaggca
    12961 aaaaccggac gttggaactt gcaaggcttt gaattgcatc attatgcagg caggctgttt
    13021 gtgttccgac tggaaaaaac ggataaactg cggtttttga aagacaggca gataagcgga
    13081 aatttaaggg aaatattgac ggggcaggga tttgttttaa aacgtcatcc gtttgggctt
    13141 cctgagcatc ttttggagca ggacggaatt ttgaggacgg tagcggtatc ggatacgttg
    13201 gctgtcggcg gcatccataa ggatgtgaaa aaaatccttc aggggaaacg ggttttgcct
    13261 gtcctgcgcc caatttggcc gcttgttgcc gacagcggaa accgtccatt ggcgttggca
    13321 aactgttgtg cggatttcca atactcggtt tcagacggca ttttgcccgt ccatcctgac
    13381 tttcccattt tattttgata atatcgcaaa cagatttcgg cggcgttcag acgggtattg
    13441 tccggttgca tatttctaaa aggcttgtga agtgaaacac atcagttcga ccaataatga
    13501 acacatccgg cacctgcacc gcctgttgtc gcaaggaaag ttcagacggc aatacgccca
    13561 aaccgttttg gagggcgtgc acctgcttca ggttttcctg caatccggca gaaaaccggt
    13621 cggggtatat attcccgagg gcaaaatgcc gtctgaagaa gtccgcaggc tgagggcggt
    13681 tgtgccggaa ggcaaggttt tttccgtttc agacggcata ttgaaaaaaa tcagcagctt
    13741 gacttgtgcg gatgatgtgc ttgcgctgat tgatattccg gatgcgggtg ctttgcctgc
    13801 cggcggcgac tgtgtggttt tggacggcgt gcaagatccg ggcaatgtcg gcaccgtctt
    13861 acggagtgcg gcggcggcag gggtaggtac ggtcgttttg ggcaggggtt gcgcggacgc
    13921 gtggtcgccc aaggtattgc gcgcggggat gggcgcgcat ttcttgtcgg gcatttatcc
    13981 gcaggcggat ttggaaatat ggttggcgcg ctataaaggc cgtgtgtttg ccaccgcctt
    14041 gcgcgaggaa aagcaggcgg ttttgtacgg cgaagatttg tgcggaccga cagcctgggt
    14101 gtttggcaac gaaggcgcgg gggtcggtaa agcggttttg gacagggcgg acaagtgtgt
    14161 caggattccg atgcacgatg caaccgagtc tttaaatgtc gcgatggcgg cgacaatctg
    14221 cctgtttgaa cagatgcggc agcggtcggc gtattgagga aaagaaatgc cgtctgaaaa
    14281 aatctattac ggcgtattga ttttcttatg tatcgcttct atgctgctgt cgccgttttt
    14341 ttatgcgggt gctttgaagc ccaagaaggc ggcattgcgg aaggacgggc agtggaaact
    14401 catcttattg tccaataccg tggcggcggc ggttttggct tgggtgtggt ggaaatggtt
    14461 ttgacagata ttgctcaaaa tcgtgctaat ggaatccgaa caaataaaga atttggtaaa
    14521 aaatttgtta aatcaacgaa ttaaagtttt gtggaaaaca aaacagctct aagcaaatag
    14581 ggcgtttgtc ggtaaatacg gaagagttgc ggcattatcg ggcatcttta acaagtagtg
    14641 ccgtcttggc aggcaatcgg tttttatggg cagcttgcaa aatcgcggat ataaaattgc
    14701 gaatcggtta aagtgtgggg acgctatgaa aaattgcgaa tttttttatg acccgacaag
    14761 ggcaatctat gatagcggtg cagattactt aactagggaa aaacatagat tagtcgtaat
    14821 tgcaaatagt gcttgggggc tattacttaa tttatcttgt tattatgacg aggttttgga
    14881 aaagcggaaa ataccgttcg gcaaacagga aattgatgac gatatggaca aagtgtccgc
    14941 ccttaagcgg aagtttaaag atatttctga aatcaaagta ggggatggtt gggaataccc
    15001 gttcaattat gagcagggaa tgaaagaatt agatgaagtg ctattgaaat acattccctt
    15061 ttttgaagaa gaacgataaa ggaggttgat atgcgcgtat ctaaaataat tggaagtatg
    15121 ttgcttgtta cagcggttca gaccgtattt tcggcaaatg tttacgcgtg ccgccataat
    15181 ggtaaaacca gttacagcca aactccggga aaacattgta ccaacgcggg tttggggcgg
    15241 gaccgggtgt acagttcggt tagaccggca gtaaaagaca gggcggaaga cgcaggggtc
    15301 ggcgattatt cggacacggt gagggacgaa cacgtccaaa atccgaaagg gaatgcacag
    15361 aaagacggtt cggatgccgg catcaggccg cattgattga aaccgaatca gccatgacgg
    15421 cgttgaataa tagaatttga acgattaggg aactctgatg aaacacatcc acattatcgg
    15481 tatcggcggc acgtttatgg gcgggattgc cgccattgcc aaagaagccg ggtttgaagt
    15541 cagcggttgc gacgcgaaga tgtatccgcc gatgagcacc cagctcgaag ccttgggtat
    15601 aggcgtacac gaaggcttcg acgcggcgca gttggaggaa tttaaagccg acgtttacgt
    15661 catcggcaat gtcgccaagc gcgggatgga tgtggttgag gcgattttga accgcgggct
    15721 gccttatatt tccggtccgc aatggctggc tgaaaacgtg ctgcaccatc attgggtact
    15781 cggtgtggcg gggacgcacg gcaaaacgac caccgcgtcc atgctcgcat gggttttgga
    15841 atatgccgga ctcgcgccgg gcttccttat cggcggcgta ccggaaaact ttagcgtttc
    15901 cgcccgcctg ccgcaaacgc cgcgtcaaga cccgaacagc caatctccgt ttttcgttat
    15961 cgaagccgac gaatacgaca ccgccttttt cgacaaacgc tccaaattcg tacattaccg
    16021 cccgcgtacc gtcgtgttga acaatctgga attcgaccac gccgacatct ttgccgactt
    16081 gggcgcgata cagacccagt tccaccacct cgtgcgcacc gtgccgtctg aaggcttaat
    16141 catctgtaac ggacagcagc aaagcttgca ggatacttta gacaaaggct gctggacgcc
    16201 ggtggagaaa ttcggcacgg aacacggctg gcaggccggc gaagccaatg ccgacggctc
    16261 gttcgacgtg ttactagacg gcaaaacggc cggacgcgtc aaatgggatt tgatgggcag
    16321 gcacaaccgc atgaatgcgc tcgccgtcat tgccgccgcg cgtcatgtcg gtgtcggcat
    16381 tcagacggcc tgcgaagcct tgggcgcgtt taaaaacgtc aaacgccgca tggaaatcaa
    16441 aggcacggca aacggcatca ccgtttacga cgacttcgcc caccacccga ccgccatcga
    16501 aaccaccata gaaggtctgc gtcagcgcgt cggaaacgcc cgcatcctcg ccgtcctcga
    16561 gccgcgctcc aacaccatga aactcggcac gatgaagtcc gccctgcctg taagcctcaa
    16621 agaagccgac caagtgttct gctacgccgg tggcgtggac tggaacgtcg ccgaagccct
    16681 cgcgcctttg ggcggcaggc tgcacgtcgg caaagacttc gatgccttcg ttgccgaaat
    16741 cgtgaaaaac gccgaagcag gcgaccatat tttggtgatg agcaacggcg gtttcggcgg
    16801 aatacacgga aagctgctgg acgctttgag atagcccggg cgatgccgtc tgaaagccct
    16861 tcagacggca tcgtccggct gcgcggcaca aaggcggaaa aaccgtttgc cccgtatttt
    16921 caaacgcgtt acacttgccg ccgctgtttt cagccatttg attacccgca accgccgtca
    16981 ttgcgccggc ggtttgcctg tcagcgtcat tgcgccgctg taaatacgaa agaacacatt
    17041 atgaccgtat cccccgtcgc cttgcgccgt aagaccgagt gcaagcctca tcccaccgcg
    17101 cgctattgga aaaaatgcga tgtcgaagcc ctgttcggac ttcccttcct cgacctcatt
    17161 taccaagccg ccgaaatcca ccgccaaaat ttcaacccgc gcgaaatcca gctttccacg
    17221 ctgttgtcca tcaaaaccgg cggttgtccc gaagactgcg cctattgtcc gcaatcggcg
    17281 caccacaata ccaatctggg caaagagcag atgatggatg tggatgaaat cgtcgaaaaa
    17341 gccaaaatcg ccaaatcgcg cggcgcaagc cggttttgta tgggcgcggc gtggcgcggc
    17401 cctaaaccca aagacgtgga gacggtttcc gcaatcatca aagccgtcaa gggcttgggt
    17461 atggaaacct gcggcacgtt cggtatgctc gaagaaggta tggcggaaga cttgaaagag
    17521 gcgggcttgg attattacaa ccacaacctc gacaccgacc ccgaccgcta caacgacatc
    17581 atccacaccc gccaacacga agaccgaatg gacaccttgg gcaaagtccg caacgccggt
    17641 ttgaaagtct gctgcggcgg catcgtcggg atgaacgaaa cccgcgccga acgtgccggg
    17701 ctgattgcca gtctcgccaa tctcgacccg cagcccgaaa gcgtgccgat taaccggttg
    17761 gtcaaagtgg aaggcacgcc gcttgccgat gccgaagatt tggactggac ggaatttgtc
    17821 cgcaccatcg ccgtggcgcg gattacgatg ccgcaaagtt atgtccggct gtcggcaggg
    17881 cgcagcaata tgcctgaagc aatgcaggcg atgtgcttta tggcgggcgc gaactcgatt
    17941 ttttacggcg acaagctgct gaccacgggc aatcctgatg aggacggcga cagaatcctg
    18001 atggaaaagc tcaacctgta tcccttgcag tttgaaccgg aaggcgaggt cgccgaagtg
    18061 gaaaaagcct ctgggattaa agtggattat tgacgattga aaaatgccgt ctgaaacccg
    18121 gaaaaaggct ttcagacggc atttgtccgg acggcatttc caatatcttt ttaccggcgc
    18181 gtgatgctgc cgtcgggcga gacatccagt ccgttcccct tggggaaatc gctgcggttc
    18241 agataaataa tccgcccgat aacggttttc tcggggtcga ttttggggat tttatcgccg
    18301 acttgagtga tggggataat gttaccggaa acgaaccgcc cctgtttgtc ggtgataatt
    18361 ttgaaaatcg gcgcgatgcc gctgatgccg gaggtgttga ttgcgccgta ggtggcaaag
    18421 ttgccgccgc tgtaggagat gaagcggtcg cggtaaagtt cgacggcgcg agtaacgtgc
    18481 ggcctctgcc cgaatacgac atccgcgccg gaatcgacgg caagccgtgc aaactcgacg
    18541 acgttgcccc tgttttcccc atagaagatt tcggtatcga acggcaggtg ttccgcctgt
    18601 ttcccttccg cgccgccgtg gaacattaca atgacgatgt cggctttctg tttggttttg
    18661 gtaatcagtt ttctaacttt ggcataatcg ttcagtttga cggcggcaag gttgggggcg
    18721 aaggagacga agccgtatct tacgccgttt ttcttcagga tggcggtttc aaacctgttt
    18781 tcgatgcccg aatatttgat gttcaattcg tcaaggttcg ccctttatgc cgtttccgca
    18841 ccgcaaaccg ccggggtcaa gccctcggcg catcctgccg gaacggaatc cccgtgctgc
    18901 cgattgacgg ttcaaacccc gcgcccgttt caaataccgg cgatgtggac ggacaggatg
    18961 cgcctgacga aaagacagcc gataccgttt ccattatcgg cgtgggcgac attatgctcg
    19021 gcagcaatta tccggtcgat tacctgcccg ataccaatat tctgaaaaac gtcgaatctg
    19081 ccttgcaaga cgcggacatt accgtcggca acctcgaagg cacgctgttt gacgaaggcg
    19141 gtacgccgaa aaaatgtgca aacccccaaa atatgctatg cattccgaac gccctccgca
    19201 tacgggcaat accttgccga cgcgggattc gactacctca gcttcgccaa caaccacagc
    19261 aacgacttcg gcgcgcaagg catcacggca acggcggcgg gcggcagctc ttttacatac
    19321 tcaatcgcgc taaagccgct gccgataacg aggccaaaat tgcggaaaat accgccatcg
    19381 cccagataaa tttgtccatc atcagacctt tactgttcag acgagacagc atttgccgca
    19441 cgttttgggg cttatctttc gatttgcgct acgtcgcgca ccgcgccttt gtcggcggaa
    19501 gtcgccatcg cgccgtaagc ccttaatgcg gcggagacgt agcggtcgcg gttttccggt
    19561 ttccacgctt tgctgccgcg tgcttccatt tcggcacggc gcgcggcaag ctcttcatcg
    19621 gaaatgacaa ggtggatgct gcggttgggg atgtcgattt cgacggtatc gccttcgtgt
    19681 accaaaccga tcgcgccacc ttccgccgct tcgggcgagg cgtgtccgat ggacaaacct
    19741 gatgtgccgc cggagaagcg tccgtcggtt aagagagcgc aggctttgcc gaggccttta
    19801 gatttcaggt aggaagtcgg atacagcatt tcctgcatgc ccgggccgcc tttcgggcct
    19861 tcgtagcgga tgatgacgat gtcgccggcg acgatttggt tgcccaaaat gccttcgaca
    19921 gcggcttcct ggctttcaaa cacgcgcgcg cggccggtga atttgaggat gctctcgtcc
    19981 acgcctgcgg tttttaccac gcagccgcgt tcggcgatgt tgccgaacaa gaccgccaaa
    20041 ccgccgtctt gcgagtaggc gtgtgccacg tcgcggatac agcctttttc gcggtcgagg
    20101 tcgagtgttt tccacatacg gttttgcgag aacgcttggg tggtgcgtac gccgccgggc
    20161 gcggctttga agcgttcgat ggcgcgggtg ttttcagggt tggttacgtc ccatttttca
    20221 atcgcgtctt tcagcgtcgg tgcgtggata gtgtgcacgt cggtgtgcag cttgcccgct
    20281 ttgtccaatt ctttcaggat ggcgaagatg ccgccggctc ggtgcacgtc ttccatatag
    20341 tagtcgtggt tgttgggtgc ggttttgcag atgcagggca caacgcggct taagcggtcg
    20401 atgtctgcca ttttgaaatc cacgcccgct tcgttggcaa cggcgagtaa atgcaaaata
    20461 gtgttggtgc tgccgcccat cgcaatatcc atcgtcatag cgttttcaaa cgcttttttg
    20521 gtggcaatgc tgcgcggtaa cacggtttca tcgttttgct cgtaatagcg tttggtgatt
    20581 tcgacaatca tacggccggc ttcgaggaac aattctttgc ggccggcgtg ggtcgccaaa
    20641 tacgaaccgt tgccgggtag ggacagaccg agtgcttcgg tcaggcagtt catcgagttt
    20701 gccgtaaaca tacccgaaca cgagccgcag gtcgggcagg cgttttgttc gacttcttca
    20761 acttgccggt tgctgacatt gtcgtccgcc gattcaatca tcgcgtcaat caagtccaaa
    20821 cggcgttcgg gctggatgtt tgccacgccg ataactttac ctgcttccat cggaccgccg
    20881 gaaacgaaga tggtcgggat gttcaggcgc atcgcggcaa tcagcatgcc cggggtgatt
    20941 ttgtcgcagt tggaaatgca caccagcgcg tcggcgcagt gggcattgac catatattcg
    21001 atggagtcgg caatcaaatc gcggctgggc agggagtaca gcatgccgct gtgtcccata
    21061 gcgatgccgt cgtcgatggc gatggtgttg aattctttgg cgatggcgcc ggctttttcg
    21121 atttcgcggg caaccagctg gcccatattg tgcaggtgga catggccggg cacgaattgg
    21181 gtgaaggagt tggcaacggc gatgatgggc ttgccgaagt cggtttccat cacgccggtg
    21241 gcgcgccaca atgcacgtgc gcccgccata ttgcggccgt gggtggaggt tttggagcgg
    21301 tattctggca tagtgtgttt ccttgtgcct ataccgtctg aaagacaggg ctgtttcaga
    21361 cggtatcggg tacggttttt tagagtggga aaagagggta ttttatacca agtatcggaa
    21421 ttttgcggga ttgaaacggc gtgcggcaaa aaagaaaatc cccgcaggaa tgcggggacg
    21481 ggttcaggcg cgggcaatcg cgacggcttt ggatgcgtcc caaaaatcaa cgggtgcatt
    21541 taatacgggt ttgacgatgc ccgtccgtat ggcgaaatcg ccgaaacctt cgccgatatt
    21601 gcgttctgcc gcccatttgc cgatcaggtc gtccaattcg gcaaggattt cgggcagggt
    21661 gatgttttct ttataaaggc gggggatgcg tacgccttca cggtcgccgc cgatatggag
    21721 gttgtagcgt tcgacggctt tgccgaccag cccgatttcc gccaacattg cccgtccgca
    21781 gccgttgggg cagccggtaa tgcgggtaac gatgtagtcg tccgacgtgc cgtgtttcgc
    21841 cataatctta tccagctcgt cgatgaagtc cggcagcacg cgttcggctt ccgccattgc
    21901 cagcgggcag gtcgggaagg aaacgcacga catcgcattt tcgcgcagtt tgctgacatc
    21961 gttgcggatt aatccgtatg ttcgggcaag ttcttcgatt tttgctttgt ctgcttcggc
    22021 gacatttgcc acgatgaggt tttggttggc ggtaatgcgg aaatcgcctt tgtggatttt
    22081 ggcgatttcc aacacgccgg tcagaagctg tttcccgcct tcgtcaacca aacggccgct
    22141 ttcgatgaaa agggtcaaat gccagttgcc gtctatgcct tttacccagc cgatgcggtc
    22201 gccgcgcccg gtaaatttga acgggcgtat gggttcgaac ggcataccca tacggcgttc
    22261 gacttcggcg cggaagttgt ccaagcccat attttgaatg gtgtagcggg tgcgggcgtt
    22321 tttgcggtcg ctgcggttgc cgaagtcgcg ctgtgtggtt accaccgctt cggcggcctt
    22381 cagcgcgtgt tccggaggta cgaaacccag ttccagtgaa atgttcggat aggttttggt
    22441 gttgccgtgt tccattgaaa gcccgccgcc tgccaaaaca ttaaaaccgg caagctgtcc
    22501 gccgccgtct gaaatggcga caaaatccaa gtcgttgccg tagcagtcca catcatttaa
    22561 gggcgggatg acgactgcgg ttttgaattt gcgcggcaga taggttttgc ccaaaatcgg
    22621 ctcgtcttct tgaaggaagt cgtcggaact ttgaactttt ttgccgtcca cccacacatc
    22681 cagataaccg cgcgtgcgcg gcagcaggtg ttcggaaatc tttttcgcgt attcgtaagc
    22741 ctgccggtgc agttcggact cgatcgggtt ggacgtgcaa agcacgttgc ggttcatatc
    22801 cgccgccgtg gcgatggaat ccaaacccag tttgtgcaag aggcggtgca tcgtctgcaa
    22861 cttggctttc ggcacgccgt gaaattggaa ggtttgccgg ttggtcagcc ggatggagcg
    22921 gtaatgactg ttttcccggg caaatttgtc cagttctatc cattgggacg gtttaatgat
    22981 cccgcccggc agccggcagc gcaaaagcat aaatttcaag ggctcgagtt ttgcctcggc
    23041 gcgttcggcg cggatgtcgc ggtcgtcctg ctcatacata ccgtggaagc ggatgagttg
    23101 gaagttgtcg cctttgaagc cgcccgtgag cgggtctttc aaatcgtcca aaatcgtgcc
    23161 gcgtaaaaaa ttgctttcgg ttttcagacg ttcgttgtcg gatagcggtt tttcttgcca
    23221 cgccaaacct tttgtcttgg tctgtacggt cattttgtgt tcctcccgat tatatttaat
    23281 caataaacat cacgctgata gcgtttttct tcgcgcagca tatccaaata ttcttctgcg
    23341 ccctcttcgt ccaaatgtcc tgccccgata atcacatcca gcaaggcggc ttccacgtct
    23401 tttgccattt ttgccgcatc gccgcacaca tagatatgcg cgccttcctg cagccattgc
    23461 caaagtcctt ccgcctgttc gcggattttg tcctgcacat agattttttc ttcctgatcg
    23521 cgggaccagg cgaaatcgta cctgtgcagg aagccgtctt tggcaaactg ctgccattcg
    23581 gtctgataga ggaagtcggc ggcaaaatgc ggattgccga aaatcagcca gtttttgcct
    23641 tccgcatttt ctgcggcacg ttgttggacg aaagcgcgga acggtgcgac gccggtgccc
    23701 gagccgatca tcacaatcgg cttgcggctg tcttcgggca gcctgaagcc gtcgttgcgt
    23761 tccacaaaca cgcgcaccgt gccgtcctct tccagccggt cggcaaggaa acccgatgcg
    23821 ccgcccaccc tggcgcgccc ttcgtgttca aaacgcaccg cgccgacggt caggtgcact
    23881 tcgtccccca cttccgcctg cgacgaggaa atcgaataca ggcgcggcgc aagcgggcga
    23941 agcaggccgg cgaattgttc cgccgtcagt tttgccggga agcggtgcag cacatcggca
    24001 atcggcgtgc tttgcacaaa gccttgcaaa acggcgttgt cggcagcaat acggtcgagt
    24061 tcgtcatcat cggcgaacgg ggcatagcct ttgacaaagg cgggggtgtt ttgcgtgagt
    24121 tcaaaatggg ataacagtgc ggaggcaacc ggcagggttt ttccgcccgc ctgtatttcc
    24181 gttgccggat cgatgccgag caggtctagg atttccctga ccagtgccgg atcgttgtca
    24241 aaccaaacgc cgagcgcgtc gcccgggagg tagtgcaaat ccgaaccgct caaatcgatt
    24301 tcgatgtggc gcacgtcttt atcggattgg cgggcggtga ttttctgatt ggccagcagg
    24361 gcggcgggaa aggggtctgc cttgcagtac ctgccatccg gtgccgtctg aaggccggcg
    24421 gggggcgttg tctgcggcgc gggcgttgcc cggttttttg cggcttcttc ttttaagcgc
    24481 gcgacaatgc ggcctgtcca tccgtctgcg gaggcggtaa agtccaaatc cgcatcaacg
    24541 cgttcgagca ggcgtttcgc ccccaattct tcaaaacgtt tgtcgaaatc tttgcccgcc
    24601 cggcagaaat tcggatagga gctgtcgccc aaacccagta cggcaaattg gagtttgtcc
    24661 aatttcgggg cttttttgcc gttcagcagt ttgtgcagca cgacggcttc ttccggcggt
    24721 tcgccttcgc cttgggtgga ggtaaccagc agcaggcggc gttcgccgga gatgtttttc
    24781 gccttatagt ctttcagttc ggcgcgcctg acttggatgc cggcggcttc caggctgtcc
    24841 gccgctttgt cggcaacgga tttcgcattg ccggtttgcg aggcggaaag gacggttacg
    24901 gaaaaaggtt ctgccgccgg caatgccgtc tgaagcgcgg gcagtcctgc agatgccccg
    24961 tttcctgctt ttgcccaagc gtagccggac agccacgccc attgtgccgc gtccagcacc
    25021 gacaggagct gcgtgatttc gggcggcaga ggcggtaatg gcggatttgt gttctgcata
    25081 tcgtgttcac tcataaaatc atacctgccg caacagtgcc gtatgtcgct tcgtctatca
    25141 ggataaacga accggcggcg gtgttttccg cataaggcgt tgccgtaacg ggtttttgaa
    25201 ggttgatgcg gactttggca atgtcgttca tcttcaagga ttccgcgccg gcctcttgtt
    25261 ccagcgtgcg gacatctaaa acgctttcaa tttccccgac ttttgccggc acggtttgcg
    25321 tgccgtgctt gagcaggtat ttgcgcgcgg tgttgagcgg acgttcgtca aaccagcaaa
    25381 gcgtggcttc cagatgtttt tgcggggcga gcggggaatt tttatcgaca aaaaggtcgc
    25441 cgcgcgaaac atcgatgtcg cggtccagcc ggagggttgc cgcctcgccg gcaaaagcct
    25501 gcgccacttc gcctttgggt gtgatgattt cagacacttt ggcggtcagc ccgttcggtt
    25561 cgatgcggac ggtttgcccg acggcgaccg aaccgcgttc gatgcgcccc tgatagcctc
    25621 ggaaatcatc ggccttgtcg gcatcttggc ggacgaccag ttgcacgggg aaataaaaat
    25681 cgtcggcggt gcggctgact tcgtccgccc ccggcagggt ttccaaaatg gacaataagg
    25741 gttcgccttt ataccaaggc atattgccgc cggggtaaac aatgttgtcg cccaagagtg
    25801 cggacaccgg tacgaaatgc acgtctttca aaccgagccg tccggcgagt cggcggtatg
    25861 cctccacaat ggcgttgaat ttgtcttcgc tgtaatccag caggtccatt ttgttgaccg
    25921 ccaccacaat atgcgggcag tcgagttggc ggaggatggc ggaatggcgt ttggtctgcg
    25981 gcagaagctg caagggctgt gcgccgaaat ccagttggga tgcgtcaacc agcacgactg
    26041 ccgccgaagc ggtgcttgcg cccgtaacca tattgcgcgt gtattgttcg tgccccggcg
    26101 tgtcggcgat gatgaatttc cgtttcgccg tggaaaaata gcggtatgcc acatcgatcg
    26161 taatgccctg ttcgcgttcg gcttccagtc cgtcggtcag gatggagaag tctatggttt
    26221 ctttcaaacc tttgcttttg ccggattcca aggttttgat ttggtcggac agcagggctt
    26281 tgctgtcgta gagcagccgt ccgatcaggg tgcttttgcc gtcatcgacg ctgccggcgg
    26341 taatgaagcg gagcggggcc tggtgttgtg ccgtcatatt ttcttcctca tatctgctta
    26401 aagggttttt gaaatttaga aatagccttc ttttttgcgt ttttccattg ccgcctcgct
    26461 tgcctgatcg tccagccggg tcgcgctgcg ttcggaaatg tcggcaaccg ctgtttctct
    26521 gataatctcc gtcggcgtgg acgcggtgct ttctaccggg caggtgcagc tgatgtcgcc
    26581 gacggtgcgg aagcggacat caaggatttc ggaggtttca gacggcattt tcggggtgag
    26641 cggcgttacg gggaccagca gccccctgcg tctgaccact tcgcgcctgt ggctgtaata
    26701 aatcggcggc agctcgaggt tttcgcgggc gatgtattgc cagatgtcga gttccgtcca
    26761 gttggaaatc gggaagacgc gcatattttc gcctttgtgc agcctggtgt tgtacagcga
    26821 ccacagctcg gggcgttgcg ccttcggatc ccattgtccg aactcgtcgc ggaacgagaa
    26881 aatccgttct ttggcgcggg ctttttcttc gtcgcgccgc gcgccgccca taagcgcgtc
    26941 gaagccgttt gcctcgatgg tttccaacaa ggtaaccgcc tgtgccgcat tgcgcgaatc
    27001 ggtttctttg cgtaagacca ccgtgccttt ggcaatggag tcttccacgc gccccactat
    27061 caggcgggca ttgagttttg ccgcctgcgc gtcgcggaaa gcaatcactt cggggtagtt
    27121 gtgccccgtg tcgatatgca ccagcgggaa gggcagtttt accggcctgc tgcccagccg
    27181 gaaggctttg caggcgaggg cgagcaggac cacggaatct ttgccgccgg aaaagagcag
    27241 ggcggggttt tcgcattccg ccgccacttc gcggataatg tggatggatt cggattccaa
    27301 ccagtcgagt tgggcgttgt tcggttcggt tttcgtcata ccatattcct tatttcttct
    27361 gtctgatatt tatgaattat ttgtgcagcc cgcattcttt gctgtttctg ccttcccacc
    27421 accaccgccc ggcgcggatg tcttcgcccg ccttgacggg gcgggtgcag gggtcgcagc
    27481 ctatgctggg aaatccttgc cggtacaaat cgttgtaagg cacattgttg gcgaggatgt
    27541 atgcccacac gtcgtgttcc gaccagtcga aaatcgggtt gtatttgccg atgccccgtc
    27601 cggcatcgta ttcggcaaac ggcagttccg tgcgtgtggc ggattgttcg cggcgttgcc
    27661 cggtaagcca ggcgtccgcg cctgcaatgg cgcggttgag cggttcgatt tttcggatgc
    27721 ggcagcattc gcggcgcgct tcaacgctgt cgtaaaaggc aaacctgcct ttgctttcca
    27781 cataacggtc ggcatcttct cgaaccggcc ggaaacgctt tatccgcaaa tggggatatg
    27841 cgcgtccgag cctgtccagc aggtttaggg tttccgtgtg gagcagcccc gtatccaagg
    27901 taaaaatgcc gatattgagg ttttcgccgg cgataaggtc ggtaatcacc atatcttccg
    27961 cagcaaggct gctggcaaac cgtgcatccc ggtggctgcc ggcaatccgg tgcaggcgtt
    28021 gtttgagggt ttccgttttt tccgcaaggg cggtttcgct gccggatccg atatgcggta
    28081 tctgccacag ggcgggtttg agcagtgtcg tttccatttt tcccgcctta tgccgcccgt
    28141 tgtccggcat tcagtccgcc caatgcggga tacgcctgcc cgacccggtt ttctccttcg
    28201 ccgttttcac cgaaccaggc gagtttttcg tgcagcccca ccacttcgcc tatgacaatc
    28261 aatgccggat tcggcgcggt ttcggcgagt tcggcaaggt tggcgagcgt gccggttgcg
    28321 gttttttgag ccggcaacgt gccttggctg atgacggctg ccggcgtgtc gggcgagcgt
    28381 ccgtgctgtt gcagccgttc ggcaatcagg gcggctttga gcgcacccat ataaatcacc
    28441 aaggtctggc ggctgcgggc gagggtctgc cattcgatgt cgggcgcatc cgccttgcgg
    28501 tggccggtta cgaaaaccgc actttgggca taatcgcggt gcgtgagcgg gatgccggca
    28561 taggcggtcg cgccgacggc ggcggtaatg ccggggacga ccgaaaacgg aatctgatgg
    28621 cgtgccaagg tttccaattc ttcgccgccg cgtccgaaca cgaaagggtc tccgcctttc
    28681 aaacgcacca cgcgcctgcc ttcgcgggcc agcctgacca taagcgcatt ggtgtcctct
    28741 tgcggggtgc ggtcgccccg ggcgcgcttg ccgacaaaaa tccgttccgc atcgcggcgg
    28801 acgagggaca gtatgccgtc tgaaaccagc gcgtcgtaaa gcaccacgtc tgcctgctgg
    28861 atttcctgta gccctttgag cgtcagcagc cccgcatcgc cgggacccgc gccgaccagc
    28921 gagacggagc cgccttgatc attttgacga ctttgttcca attggcctgc caattcccgt
    28981 tcggcaaggg tgttttgccg gtttttgacg agggcggcga aacgtccgtt aaactgcttt
    29041 tcccaaaagc ggcggcgttc ggtaacggat ttcagtttgt ccttgacggc atcgcgccac
    29101 cttcctgaaa tttccgccat atcgcccaaa gacggcggca gcagggcttc cagcctttca
    29161 cgcagcagtc gggcgaggac gggcgcgctg ccggagctgg aaacggcaat ctgaaccggg
    29221 ttgcggtcga taaccgacgg gaagatgaag ctgcaatggt cgcggtcgtc caccacgttg
    29281 accggctttt ggcagctttc ggcaagatgg aaaacgcgcc ggttgagggc ttggtcgctg
    29341 cttgccgcaa tgatgaggaa aactgtgcgg atgtgttcgg cacgaaattc ttcggcaagc
    29401 cacaggattt tgttttccgc cgccaacgcg gagagttcgg cattcaggtg ttttgcggca
    29461 accctgacct ctgcgcccgc cttcagcagc aggctgattt tgcgtgcggc gaccgcgccg
    29521 ccgcctacga ccaatacggg gcggccggcg aggttggcga aaatagggaa ataattcact
    29581 ggctgactcc tttgctgttt gcccgcacct tgtttccgat acggtgcgtc gcggcatttt
    29641 tgtcggaatg cgggtcattt tagacaaaag gattttcccg gttaaataat aaaaaggcat
    29701 ttgttagaag ctgaaagcta tatgagggcg gctgcggatg cggcggtttt cc
//
LOCUS       DAXHPG010000005        29117 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXHPG010000005 DAXHPG010000000
VERSION     DAXHPG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29117
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.005"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            24..1106
                     /gene="mraY"
                     /locus_tag="ACFM6F_000158"
     CDS             24..1106
                     /gene="mraY"
                     /locus_tag="ACFM6F_000158"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC6163659.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVAGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            1221..1493
                     /locus_tag="ACFM6F_000159"
     CDS             1221..1493
                     /locus_tag="ACFM6F_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFC6163660.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDLVLAQEWFGKACQNGDQDGCDIYQRLKAGY"
     gene            1583..2920
                     /gene="murD"
                     /locus_tag="ACFM6F_000160"
     CDS             1583..2920
                     /gene="murD"
                     /locus_tag="ACFM6F_000160"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC6163661.1"
                     /translation="MTLENKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            3057..4370
                     /locus_tag="ACFM6F_000161"
     CDS             3057..4370
                     /locus_tag="ACFM6F_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC6163662.1"
                     /translation="MTGGFLKISEVLVKVGDGVHTLLLDRQIVRDGRKFDAPLLWMVV
                     LMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRL
                     VPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRRE
                     EVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLI
                     MVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAF
                     LDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFG
                     FFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGA
                     LPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            4374..5441
                     /gene="murG"
                     /locus_tag="ACFM6F_000162"
     CDS             4374..5441
                     /gene="murG"
                     /locus_tag="ACFM6F_000162"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC6163663.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPAPAERFQGRKGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            5596..7005
                     /gene="murC"
                     /locus_tag="ACFM6F_000163"
     CDS             5596..7005
                     /gene="murC"
                     /locus_tag="ACFM6F_000163"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC6163664.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            7118..8032
                     /locus_tag="ACFM6F_000164"
     CDS             7118..8032
                     /locus_tag="ACFM6F_000164"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC6163665.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            8022..8750
                     /locus_tag="ACFM6F_000165"
     CDS             8022..8750
                     /locus_tag="ACFM6F_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC6163666.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            8836..10080
                     /gene="ftsA"
                     /locus_tag="ACFM6F_000166"
     CDS             8836..10080
                     /gene="ftsA"
                     /locus_tag="ACFM6F_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC6163667.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLQRWIENSF"
     gene            10199..11377
                     /gene="ftsZ"
                     /locus_tag="ACFM6F_000167"
     CDS             10199..11377
                     /gene="ftsZ"
                     /locus_tag="ACFM6F_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC6163668.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            11516..12826
                     /locus_tag="ACFM6F_000168"
     CDS             11516..12826
                     /locus_tag="ACFM6F_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC6163669.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            13451..14326
                     /gene="prpB"
                     /locus_tag="ACFM6F_000169"
     CDS             13451..14326
                     /gene="prpB"
                     /locus_tag="ACFM6F_000169"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC6163670.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            14411..15565
                     /gene="prpC"
                     /locus_tag="ACFM6F_000170"
     CDS             14411..15565
                     /gene="prpC"
                     /locus_tag="ACFM6F_000170"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC6163671.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEDR"
     gene            15977..16699
                     /locus_tag="ACFM6F_000171"
     CDS             15977..16699
                     /locus_tag="ACFM6F_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFC6163672.1"
                     /translation="MDKDKGSYAGIPVQNTKELVWQKIQEIPISECGEPLVSVGNTGR
                     IRVNPVYFSQGIEGAINTVLLRRSVADRLTQVVSLLPEMYGLEIFDGWRPVIVQRALR
                     ENMKQEIEKRHPEYDEVQVREALDCFAADPDRAGMPLPHLTGGSVDVGLFDIKSGKSL
                     DMGSEFDATGHLSYSDALEKEGEYFSSARNLRRILIHAMKEAGFSNLPTEWWHFDYGN
                     QNWAYFTQQSNAVFGAVEEQDT"
     gene            16725..17930
                     /locus_tag="ACFM6F_000172"
     CDS             16725..17930
                     /locus_tag="ACFM6F_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFC6163673.1"
                     /translation="MQTLISADNHLLLWAFVMLAAAAAIFIEQNSRLAGKVPGAVLAL
                     LIALIASNLGVIPTDAPVFDAVWSYIVPLAIPLLLFQLDLHALFKESGRMLFIFLISS
                     LGTVIGTLVSFALLKNYIPELDKVAGMISASYTGGGVNFAAMSAKLNPAKDVIAATIV
                     ADNLMMACYFFILIGISSSYWVRKVWGSPYQDKLEQEYHDPSVNKAAEYWKPKSISLQ
                     SIALSLAASILLVAVSFSLSSFLQNHFQSVDGVALKMLTGLISDKYLLLTTFTLAVVL
                     FFRKGIQKLDGSQELGTYCIYLFFVVIGVPASIPLIIKTAPLLFVFTLIIAVLNLLLT
                     LVLGKLFKFSIEEIVLACNANIGGPTTAAALAISKGWRDLVGAILLIGTVGYIIGNYI
                     GSFVYALLS"
     gene            18034..20640
                     /gene="acnD"
                     /locus_tag="ACFM6F_000173"
     CDS             18034..20640
                     /gene="acnD"
                     /locus_tag="ACFM6F_000173"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HFC6163674.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRQPGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAGKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLQRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGVVA"
     gene            20727..21173
                     /locus_tag="ACFM6F_000174"
     CDS             20727..21173
                     /locus_tag="ACFM6F_000174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163675.1"
                     /translation="MKTQEDEVTKQKIFNHMINNKSFLNFQFWTFAALSAGSFAFLIS
                     AANIDSKDRDLTLEIAIVGYSVSLILNSILCFLIRGIEDDELSVKMLNEFNLDKRTFW
                     IPILATWSFALSTLFLLFTISLVGAITALIMAVLAIISFIKFTKKS"
     gene            21337..22506
                     /gene="prpF"
                     /locus_tag="ACFM6F_000175"
     CDS             21337..22506
                     /gene="prpF"
                     /locus_tag="ACFM6F_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HFC6163676.1"
                     /translation="MPQIKIPAVYYRGGTSKGIFFKRTDLPEAAQQAGEARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            22920..24119
                     /locus_tag="ACFM6F_000176"
     CDS             22920..24119
                     /locus_tag="ACFM6F_000176"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6163677.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            24227..24847
                     /locus_tag="ACFM6F_000177"
     CDS             24227..24847
                     /locus_tag="ACFM6F_000177"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6163678.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKVDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(24974..25417)
                     /locus_tag="ACFM6F_000178"
     CDS             complement(24974..25417)
                     /locus_tag="ACFM6F_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC6163679.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            25591..25959
                     /locus_tag="ACFM6F_000179"
     CDS             25591..25959
                     /locus_tag="ACFM6F_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6163680.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            26004..27158
                     /locus_tag="ACFM6F_000180"
     CDS             26004..27158
                     /locus_tag="ACFM6F_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6163681.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(27215..28087)
                     /locus_tag="ACFM6F_000181"
     CDS             complement(27215..28087)
                     /locus_tag="ACFM6F_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC6163682.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATFPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            28172..28984
                     /locus_tag="ACFM6F_000182"
     CDS             28172..28984
                     /locus_tag="ACFM6F_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC6163683.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKHWVLDAV"
BASE COUNT         6795 a         7314 c         7981 g         7027 t
ORIGIN      
        1 acaaaacaaa aaaaggaagc cccatgtttt tatggctcgc acatttcagc aactggttaa
       61 ccggtctgaa tatttttcaa tacaccacat tccgcgccgt catggcggcg ttgaccgcct
      121 tggcgttttc cctgatgttc ggcccgtgga cgatacgcag gctgaccgcg ctcaaatgcg
      181 ggcaggcagt gcgtaccgac ggcccgcaaa cccacctcgt caaaaacggc acgccgacga
      241 tgggcggttc gctgattctg accgccatta ccgtgtccac cctgttgtgg ggcaactggg
      301 cgaacccgta tatctggatt ctcttgggcg tattgctcgc taccggcgca ctcggttttt
      361 acgacgactg gcgcaaagtc gtctataaag accccaacgg cgtgtccgcc aaattcaaaa
      421 tggtgtggca gtcaagcgtt gccattatcg ccggtttggc attgttttac cttgccgcca
      481 attccgccaa caatattttg attgtcccgt tcttcaaaca aatcgccctg ccgctgggcg
      541 tggccggctt tttggtgttg tcttacctga ccatcgtcgg cacatccaac gccgtcaatc
      601 tcaccgacgg cttggacggc cttgccgcct tccccgtcgt cctcgttgcc gccggactcg
      661 ccattttcgc ctacgtcagc ggacactacc aattttccca atacctgcaa ttaccttacg
      721 ttgccggcgc aaacgaagtg gtgattttct gtaccgccat gtgcggcgcg tgcctcggtt
      781 tcttgtggtt taacgcctat cccgcgcaag tctttatggg cgatgtcggt gcattggcat
      841 tgggtgccgc gctcggtacc gtcgccgtca tcgtccgcca agagtttgtc ctcgtcatta
      901 tgggcggatt atttgtcgta gaagccgtat ccgttatgct tcaggttggc tggtataaga
      961 aaaccaaaaa acgcatcttc ctgatggcgc ccatccatca ccactacgaa caaaaaggct
     1021 ggaaagaaac ccaagtcgtc gtccgctttt ggattattac catcgtcttg gtgttgatcg
     1081 gtttgagtac cctcaaaatc cgctgaacct atgccgtctg aacacctttc agacggcatt
     1141 tgaacgcgca ataaacctgc ggcgacaatc cgcccagccc tatcgttaac ggcggcttat
     1201 actaaaacag aagtaaaacc atgaaacaga cagtcaaatg gcttgccgcc gccctgattg
     1261 ccttgggctt gaaccaagcg gtgtgggcgg atgacgtatc ggattttcgg gaaaacttgc
     1321 aggcggcagc acagggaaat gcagcagccc aatacaattt gggcgcaatg tattacaaag
     1381 gacgcggcgt gcgccaagac cttgtgctcg ctcaagaatg gtttggtaag gcttgtcaaa
     1441 acggagacca agacggctgc gacatttacc aacgcctgaa ggcaggttat tgaacagccc
     1501 gcgatgccgt ctgaaagcgg cttgggcagg ggcggacatc cccccacctg cccaatatga
     1561 tttgttttag gacaaaacca aaatgacgct tgaaaacaaa aaaatcctcg tcgccggact
     1621 cggcggtacg ggtatttcca tgattgccta tctgcgcaaa aacggcgcgg aggtggccgc
     1681 ttatgatgcg gagttgaagc ccgagcgcgt gtcgcaaatc ggtaagatgt ttgacgggct
     1741 ggtgttttac acaggccgtc tgaaagatgc gctggacaac ggtttcgata ttttggcact
     1801 cagccccggc atcagcgagc gtcagccgga tattgaggcg ttcaagcaaa acggcggacg
     1861 cgtgttgggc gacatcgaat tgctggcgga cattgtgaac cgccggggcg acaaggtaat
     1921 tgcgattacc ggcagcaacg gcaaaaccac ggtaacgagc ctggtcggct atctctgtat
     1981 caagtgcggg ctggataccg ttatcgcggg caatatcggc acgccggttt tggaggcgga
     2041 atggcagcgc gaaggcaaaa aggcggacgt gtgggtgttg gagctttcca gcttccaact
     2101 ggaaaacacc gaaagcctgc gcccgacggc ggcgacggta ctgaatattt ccgaagacca
     2161 tctcgaccgc tacgacgact tgctcgacta cgcgcatacc aaagccaaga ttttccgtgg
     2221 cgacggtgtg caggttttga atgcggacga tgcgttctgc cgtgccatga aacgtgccgg
     2281 gcgcgaggta aaatggtttt cgttggaaca cgaagccgat ttttggttgg aacgtgagac
     2341 aggccgcctg aaacaaggca atgaagattt gattgccacg caagatattc cgttgcaagg
     2401 cttgcacaac gccaccaacg tgatggctgc cgttgccttg tgcgaagctg tcggtttgcc
     2461 gcgcgaagca ttgctcgaac acgtcaaaac ctttcaaggc ctgccgcacc gcgtggaaaa
     2521 aatcggcgag aaaaatggcg tggtgtttat cgatgacagc aaaggcacga acgtcggtgc
     2581 gaccgccgcc gcgattgccg gtttgcaaaa tccgctcttc gtgattttag gcggcatggg
     2641 caaagggcag gacttcacgc ctctgcgcga cgcgcttgcc ggcaaggcaa aaggcgtgtt
     2701 cctgatcggc gttgatgcgc cgcaaatccg ccgcgatttg gacggctgcg gtctgaatat
     2761 gaccgactgc gccactttgg aagaagccgt tcagacggca tatgcccaag ccgaagcgtg
     2821 cgatatcgtg ctgctcagcc cggcttgtgc gagttttgat atgtttaaag gctacgcgca
     2881 ccgttcggaa gtgtttatcg aagcgtttaa ggctttgtga tgccgtctga aatgcaaacg
     2941 ccgtcattgt tgggcggcaa gtaaagattt agaataccga tttgggatgt atcgtatgtt
     3001 cggacggcat tgtctgccgt ctgaaatttt tgccctttgc ggcaggtgca aacagaatga
     3061 caggtggttt tttgaagatt tcggaagtat tggtaaaagt gggcgacggt gtccacactc
     3121 tgctgctcga caggcagatt gtgcgcgacg gcaggaaatt cgacgcgccg cttttgtgga
     3181 tggtggtgct gatgacggcg ttcagcctgc tgatgattta ttcggcttct gtgtatttgg
     3241 catcaaaaga aggcggcgat cagtttttct atttgaccag acaggcgggg ttcgtcgttg
     3301 ccggcttgat agcgagcggt tttttatggt ttctttgcag gatgaggaca tggcggcggc
     3361 ttgtgccgtg gatttttgcc ttatccggcc tgttgctggt agtcgtattg attgccgggc
     3421 gcgaaatcaa tggcgcgacc cgttggatac ctttgggtcc gttgaatttc cagccgaccg
     3481 agctgttcaa gctggcggtc atcctttatt tggcaagcct gttcacgcgc cgtgaagaag
     3541 tgttgcgcag catggaaagt ttgggttggc agtcgatttg gcgggggacg gccaatctga
     3601 tcatgtccgc caccaatccg caggcacgtc gtgaaacatt agaaatgtac ggccgtttcc
     3661 gggcgatcat cctgccgatt atgctggtgg cgttcggttt ggtgctgata atggtacagc
     3721 cggatttcgg ttcgtttgtc gtcattaccg tcattgccgt tggaatgctg tttttggcag
     3781 gattgccgtg gaaatatttt ttcgtcctgg taggcagcgt cttgggcggg atggtgctga
     3841 tgattaccgc cgctccctac cgtgtgcagc gggtagtggc atttttggac ccgtggaaag
     3901 acccgcaggg tgccggctac cagcttaccc actctctgat ggcaatcggg cgcggagagt
     3961 ggttcggtat gggtttgggt gcgagtttga gcaaacgcgg ctttctgccg gaagcgcata
     4021 ccgattttat ttttgccatc atcgccgaag aattcggttt cttcggtatg tgcgtgctga
     4081 tattctgtta cggctggctg gtggtgcggg cgttttccat cggcaagcag tcgcgcgatt
     4141 tgggtttgac tttcaacgcc tatatcgctt cgggtatcgg catttggatc ggtatccaaa
     4201 gtttcttcaa tatcggtgtg aacatcggtg ctttgccgac caaaggtctg acgctgccgt
     4261 tgatgtccta tggcggttcg tcagtctttt tcatgctgat cagcatgatg ctgctgttgc
     4321 gtatagatta tgaaaaccgc cggaaaatgc gcggttatcg ggtggagtaa atcatgggcg
     4381 gtaaaacctt tatgctgatg gcgggcggaa cgggcggaca tattttcccc gcgctggcgg
     4441 tggcggattc attgcgcgcg cgcggccatc atgtgatttg gctgggcagc aaggattcga
     4501 tggaagagcg tatcgtgccg caatacggca tacgcttgga aacgctggcg attaaaggcg
     4561 tgcgcggcaa cggcatcaaa cgcaagctga tgctgccgtt tactttgtat caaaccgtcc
     4621 gcgaagcgca gcggattatc cgcaaacacc gtgtcgagtg cgtcatcggc ttcggcggct
     4681 tcgttacctt ccccggcggt ttggcggcga agctattagg cgtgccgatt gtgattcacg
     4741 agcaaaacgc cgtggcaggt ttgtccaacc gccacctgtc gcgctgggcg aagcgggtgt
     4801 tgtacgcttt tccgaaagcg ttcagccacg aaggcggctt ggtcggcaac cccgtccgcg
     4861 ccgatattag caacctgccc gcgcctgccg aacgcttcca agggcgtaaa ggccgtctga
     4921 aaattttggt ggtcggcggc agtttgggcg cggacgtttt gaacaaaacc gtaccgcagg
     4981 cattggcttt gctgcccgac aatgcgcgtc cgcagatgta ccaccaatcg ggacggggca
     5041 agctgggcag cttgcaggcg gattacgacg cgctgggcgt gaaagccgaa tgcgtggaat
     5101 ttattaccga tatggtgtcc gcctaccgcg atgccgattt ggtgatttgc cgtgccggcg
     5161 cgctgacgat tgccgagttg acggcggcgg gattgggtgc gttgttagtg ccgtatcctc
     5221 acgccgttga tgaccatcaa accgccaacg cgcgttttat ggtgcaggca gaagcaggac
     5281 tgctgttgcc gcaaacccag ttgacggcgg aaaaactcgc cgaaatcctc ggcagcctga
     5341 accgcgaaaa atgtctcaaa tgggcggaaa acgcccgtac tttggcattg ccgcacagcg
     5401 cggacgacgt tgccgaagcc gcgattgcgt gtgcggcgta aactgccgaa ccatgccgtc
     5461 tgaaatgccg tccagacagc atggatgttt tttatttcaa tccgctatat atttgtcaga
     5521 aaactatggc gcgcaaacgg tcagcccttt aaaataacgc ctttacgcat cgaaaatcca
     5581 ccggaacgca acattatgat gaaaaatcga gtgaccaaca tccattttgt cggtatcggc
     5641 ggcgtcggca tgagcggcat cgccgaagtc ttgcacaatt tgggttttaa agtttccggt
     5701 tcggatcagg cgcgtaatgc cgctaccgag catttgggca gcctgggcat tcaagtttat
     5761 cccggccata ccgcagaaca cgttaacggt gcggatgtcg tcgttacctc taccgccgtc
     5821 aaaaaagaaa atcccgaagt tgtcgctgcg ttggagcagc aaattcccgt tattccgcgc
     5881 gccctgatgt tggcggagtt gatgcgcttc cgtgacggca tcgccattgc cggcacgcac
     5941 ggcaaaacca cgaccaccag cctgaccgcc tccatcctcg gcgcggcagg acttgacccg
     6001 actttcgtta tcggcggcaa actcaacgcc gcaggcacca acgcccgctt gggcaaaggc
     6061 gaatacatcg ttgccgaagc cgacgagtcg gatgcatcct ttctgcacct gacaccgatt
     6121 atgtccgtcg ttaccaatat cgacgaagac catatggata cctacgggca cagcgtcgaa
     6181 aaactgcatc aggcgtttat cgatttcatc caccgtatgc ccttctacgg caaagccttt
     6241 ttgtgtattg acagcgaaca cgtccgcgcg attttgccca aagtgagcaa accttatgct
     6301 acttacggtt tggacgatac cgccgacatc tacgccaccg acatcgaaaa cgtcggcgcg
     6361 caaatgaaat tcaccgtcca tgttcaaatg aaaggacatg agcaggggtc gtttgaagtc
     6421 gtgctgaata tgcccggcag acacaacgtg ctgaacgcat tggcagccat cggcgtggcg
     6481 ctggaagtcg gcgcatcggt tgaagcgatc caaaaaggct tgctcggctt tgaaggtgtc
     6541 ggccgccgct tccaaaaata cggcgacatc aagttgccaa acggcgggac cgcgctcttg
     6601 gtggacgact acggacacca ccccgtcgaa atggcggcga cccttgccgc cgcacgcggc
     6661 gcgtatccgg aaaaacgttt ggtactcgcc ttccagccgc accgctatac ccgcacgcgc
     6721 gatttgtttg aagactttac caaagtcctc aataccgttg acgcgctggt gctgaccgaa
     6781 gtttatgccg ccggtgaaga gccgattgcc gccgccgatt cccgcgctct tgcccgcgcc
     6841 atccgcgtgt tgggcaaact cgagccgatt tactgcgaaa acgttgccga tctgcccgaa
     6901 atgctgttga acgttttgca ggacggcgac atcgtgttga atatgggtgc gggaagcatc
     6961 aaccgcgtcc ccgccgcgct gctggaattg tcgaaacaga tttgaggcac acccgcctga
     7021 cagacggaac atcatataaa ggtcgtctga aaccgcaaat caggtttcag acgacctctg
     7081 gcaacaagca taaagcaatc aggaaagaac aaaaacaatg cagaattttg gcaaagtggc
     7141 cgtattgatg ggcggttttt ccagcgaacg agaaatctcg ctggacagcg gcaccgccat
     7201 tttgaatgct ttaaaaagca aaggcataga cgcatatgcc ttcgatccta aagaaacccc
     7261 attgtctgaa ttgaaggcac aaggttttca gacggcattc aacatccttc acggtactta
     7321 cggcgaagac ggggcggttc agggtgcatt ggaactgttg ggcattccct ataccggcag
     7381 cggtgtcgcc gcatccgcca tcggcatgga caaataccgc tgcaaactga tttggcaggc
     7441 attgggactg cccgttcccg agttcgccgt cctgcacgac gacactgatt tcgatgccgt
     7501 cgaagaaaaa ttgggcctgc cgatgtttgt gaaaccggcg gccgaaggca gcagcgtagg
     7561 cgtggtaaaa gtcaaaggaa aaggccgtct gaaaagcgtt tacgaagaat tgaaacacct
     7621 tcagggcgaa atcattgccg aacgttttat cggcggcggc gaatattcct gccccgtcct
     7681 gaacggcaaa gggctgcccg gcatacacat cattcccgca accgagtttt acgactacga
     7741 agccaagtac aaccgcgacg acaccattta tcaatgtcct tcggaagatt tgaccgaagc
     7801 cgaagaaagc ctgatgcgcg aactggcggt tcgcggcgcg caggcaatcg gtgcggaagg
     7861 ctgcgtgcgc gtcgatttcc tcaaagatac cgacggcaaa ctctatctgt tggaaatcaa
     7921 caccctgccc ggtatgacga gccatagttt agtaccgaaa tccgctgccg ttacgggcgt
     7981 gggttttgcc gatttatgta ttgaaatttt gaagaccgca catgtgggat aatgccgaag
     8041 cgatggaacg gctgacgcgc tggctgcttg tcatgatggc gatgctgctt gctgcgtccg
     8101 ggctggtttg gttttacaat tcgaatcatc tgcccgtcaa gcaggtgtcg ctgaagggca
     8161 acctggttta ttccgataag aaggcattgg gcagtttggc gaaagaatac atccatggga
     8221 atattttgag gacggacatc aatggcgcac aggaggccta ccgccggtat ccgtggattg
     8281 cgtcggtcat ggtgcgccgc cgttttcccg acacggttga ggtcgtcctg accgagcgca
     8341 agccggtcgc gcgttggggc gaccatgcct tggtggacgg cgaaggcaat gtttttgaag
     8401 cccgcttgga cagacccgga atgccggtat tcagaggcgc ggaaggaacg tctgccgaaa
     8461 tgctccgccg ttatgacgaa ttttcgactg ttttggcaaa acagggtttg ggcatcaaag
     8521 agatgaccta tacggcacgt tcggcgtgga ttgtcgtttt ggacaacggc atcaccgtca
     8581 ggctcggacg ggaaaacgag atgaaacgcc tccggctttt taccgaagcg tggcagcatc
     8641 tgttgcgtaa aaataaaaat cggttatcct atgtggatat gaggtataag gacggatttt
     8701 cagtccgcta tgcttccgac ggtttacccg aaaaagaatc cgaagaatag tgggaacagg
     8761 tatcggacag attacggccg tgccgtctga aacggtgcga cgcaaatttc aatcagtttt
     8821 aagagcagac gaacaatgga acagcagcaa agatacatca gcgtactgga tatcggtacg
     8881 tctaaagtcc tcgcactgat cggggaagtt caagatgacg acaaaatcaa catcgtcggt
     8941 ttggggcagg ctccttcacg gggcttgcgc gcgggcatgg taaccaatat cgatgccacc
     9001 gtccaagcca tcaggcaggc ggtcaatgat gccgagctga tggcggatac caaaattacc
     9061 cacgttacca caggtatcgc aggcaaccac atccgcagtc tcaattcgca aggtgtggtt
     9121 aaaattaaag acggggaagt cacgcaggca gacatcgacc gcgccattga aacggcaaag
     9181 gcaatcaata tcccgcccga tcaaaaaatt ctcgatgccg tggttcaaga ctacattatc
     9241 gacacccaac ttggcgtgag ggagcccatc ggtatgagcg gtgtgcgtct ggatacgcgg
     9301 gtgcacatca ttaccggtgc aagtacggca gtgcagaatg tccaaaaatg tatcgagcgg
     9361 tgcggtttga aaagcgatca gattatgctt cagccgttgg caagcgggca ggcggtgctg
     9421 actgaagatg aaaaagacct cggtgtatgc gtcatcgaca ttggtggcgg aacgaccgat
     9481 attgccgttt atatgaacgg cgccatccgc catacgtccg tcattccggc cggtggtaat
     9541 ctgattacca aagatttgtc caaatcgttg agaacacctc tcgatgccgc cgagtacatt
     9601 aaaatccatt atggcgtggc atcatgcgat acggaaggct tgggtgagat gattgaagtt
     9661 ccgggcgtgg gtgaccggac atcgcgtcag gtttccagta aggttttggc agcaatcatc
     9721 agtgcacgta ttcaggagat ttttggcgta gtgctgggcg agctgcaaaa atcgggtttc
     9781 cccaaagaag tgctgaatgc gggtatcgtt ctgaccggcg gtgtgtccat gatgaccggg
     9841 attgtggaat ttgccgaaaa aatcttcgat ttgcctgtac gcaccggtgc accccaagaa
     9901 atgggcggtt tgtccgaccg cgtccgcaca ccgcgttttt ctaccgctat cgggctgctt
     9961 catgcagcat gcaagctgga aggaaacttg ccgcagccgg aaaacggtgc agtgcaagag
    10021 agggaagggg gcggcggttt gttggcaaga ttgcaacggt ggattgaaaa cagcttctga
    10081 acaggtggat tgccgtttga caggtgagaa gtattttgcc agcagcaaga tacttcttat
    10141 ataatgaata ataatttatt taaaccgtcc tctgaatggg gcgagcagga gtttttgaat
    10201 ggaatttgtt tacgacgtgg cagaatcggc agtcagccct gcggtgatta aagtaatcgg
    10261 cttgggcggc ggcggttgca atgcaatcaa taacatggtt gccaacaatg tgcgcggtgt
    10321 ggagtttatc agtgccaata cggatgcgca gtctctggca aaaaaccatg cggcgaagag
    10381 aatccagttg ggtacgaatc tgacacgcgg tttgggcgcg ggcgcgaatc ccgatatcgg
    10441 ccgtgcggca gcccaggaag accgggaagc cattgaagaa gccattcgcg gtgcgaatat
    10501 gctgtttatc acgaccggta tgggcggcgg taccggtacc ggttccgcgc cggttgttgc
    10561 tgagattgcc aagtctttgg gcattctgac cgttgccgtg gttacccgac cgttcgcata
    10621 tgaaggtaag cgcgtccatg tcgcacaggc agggttggaa cagttgaaag aacacgtcga
    10681 ttcgctgatc atcatcccga acgacaaact gatgactgca ttgggtgaag acgtaacgat
    10741 gcgcgaagcc ttccgtgccg ccgacaatgt attgcgcgat gcggtcgcag gcatttccga
    10801 agtggtaact tgcccgagcg aaatcatcaa cctcgacttt gccgacgtga aaaccgtgat
    10861 gagcaaccgc ggtatcgcta tgatgggttc gggttatgcc caaggtatcg accgtgcgcg
    10921 tatggcgacc gaccaggcca tttccagtcc gctgctggac gatgtaacct tggacggagc
    10981 gcgcggtgtg ctggtcaata ttacgactgc tccgggttgc ttgaaaatgt ccgagttgtc
    11041 cgaagtcatg aaaatcgtca accaaagcgc gcatcccgat ttggaatgca aattcggtgc
    11101 ggctgaagac gagaccatga gcgaagatgc catccggatt accattatcg ctaccggtct
    11161 gaaagaaaaa ggcgcggtcg attttgttcc ggcaagggag gtagaagcgg ttgccccgtc
    11221 caaacaggag caaagccaca atgtcgaagg tatgatccgc accaatcgcg gtatccgcac
    11281 gatgaacctt accgctgcgg atttcgacaa tcagtccgta cttgacgact ttgaaatccc
    11341 tgctattttg cgtcgtcaac acaattcaga caaataatgt gctgtttgcc cgtaaacctg
    11401 ctgcctcccg aatcggtttg tccggtttgg gaggtatgtt tttcaagatg ttgcaatttc
    11461 gtacggtttg cggtcggcgg attcagattt ttccacttga tacagacttt cagatatgga
    11521 cacttcaaaa caaacactgt tggacgggat ttttaagctg aaggcaaacg gtacgacggt
    11581 gcgtaccgag ttgatggcgg gtttgacaac ttttttgacg atgtgctaca tcgttatcgt
    11641 caaccctctg attttgggcg agaccggcat ggatatgggg gcggtattcg tcgctacctg
    11701 tatcgcgtct gccatcggct gttttgttat gggttttgtc ggcaactatc cgattgcact
    11761 cgcaccgggg atggggctga atgcctattt cacctttgcc gtcgttaagg gtatgggcgt
    11821 gccttggcag gttgcgttgg gtgcggtgtt catctccggt ctgattttca tcctgttcag
    11881 cttttttaaa gtcagggaaa tgctggtcaa cgcactgcct atgggtttga aaatgtcgat
    11941 tgctgccggt atcggtttgt ttttggcact gatttccctg aaaggcgcag gcattatcgt
    12001 tgctaatccg gcaaccttgg tcggtttggg cgatattcat cagccgtccg cgttgttggc
    12061 actgttcggt tttgctatgg tggtcgtatt gggacatttc cgcgttcaag gcgcaatcat
    12121 catcaccatt ttgacgatta ccgtcattgc cagcctgatg ggtttgaatg aatttcacgg
    12181 catcatcggc gaagtaccga gcattgcgcc gacttttatg cagatggatt ttgaaggcct
    12241 gtttaccgtc agcatggtca gtgtgatttt cgtcttcttc ttggtcgatc tgttcgacag
    12301 taccggaacg ctggtcggca tatcccaccg tgccgggctg ctggtggacg gtaagctgcc
    12361 ccgcctgaaa cgcgcactgc ttgcagactc taccgccatt gtggcaggtg cggctttggg
    12421 tacttcttcc accacgcctt atgtggaaag tgcggcgggc gtatcggcag gcggacggac
    12481 cggcctgacg gcggttaccg tcggcgtatt gatgctcgcc tgcctgatgt tttcaccttt
    12541 ggcgaaaagt gttcccgctt ttgccaccgc gcccgccctg ctttatgtcg gcacgcagat
    12601 gctccgcagt gcgagggata ttgattggga cgatatgacg gaagccgcac ccgcattcct
    12661 gaccattgtc ttcatgccgt ttacctattc gattgcagac ggcatcgcct tcggcttcat
    12721 cagctatgcc gtggttaaac ttttatgccg ccgcaccaaa gacgttccgc ctatggtatg
    12781 gattgttgct gtattgtggg cactgaaatt ctggtatttg ggctgattga ttcgatatta
    12841 aaaatgccgt ctgaaaggtt ttcagacggc attttgtttg ccggtatatt aaatttttat
    12901 taaattatat aaaaatcaaa tagataataa actacatcag attacttaaa aataatacat
    12961 tgtttttatg tataaaatat tttataaggt ttcaggattt tgattatcga aaatttttct
    13021 tgatttcctg acaattttat tgaaacaaat aattcaaaat taatctagtt taatcatgga
    13081 attaaaataa aatattaaaa ttatgtaatg agtctcctta aaaatgtttg acattttcag
    13141 tcttgtgttt tagattagcg aaaaataaaa ctacacaata ctacaaagga acattactat
    13201 gaaaccaatt cagatgtttt ccccttttct gaataatccc cttgttttct tcttgtctgc
    13261 ggttttgccg cataattccg aacggtctgc tgtttttctt tgattcgttt taaatatcaa
    13321 taagataatt gttctcatat atttttaatg attggattgg gatgcccgac gcgtcggatg
    13381 gctgtgtttt gccgtccgaa tgtgatggaa gcctgtccat actgaaaaaa gtctataaag
    13441 gagaaatatg atgagtcaac actctgccgg agcacgtttc cgccaagccg tgaaagaatc
    13501 gaatccgctt gccgtcgccg gttgcgtcaa tgcttatttt gcacgattgg ccacccaaag
    13561 cggtttcaaa gccatctatc tgtccggcgg cggcgtggca gcctgttctt gcggtatccc
    13621 tgatttgggc attaccacaa tggaagatgt gctgatcgac gcacgacgca ttacggacaa
    13681 cgtggatacg cctctgctgg tggacatcga tgtgggttgg ggcggtgcat tcaatattgc
    13741 ccgtaccatt cgcaactttg aacgcgccgg tgttgcagcg gttcacatcg aagatcaggt
    13801 agcgcaaaaa cgctgcggcc accgtccgaa caaagccatt gtatctaaag atgaaatggt
    13861 cgaccgtatc aaagctgccg tagatgcgcg cgttgatgag aacttcgtga ttatggcgcg
    13921 taccgatgcg ctggcggtag aaggtttgga tgccgctatc gaacgcgccc aagcttgtgt
    13981 cgaagccggt gcggacatga ttttccctga agccatgacc gatttgaaca tgtaccgcca
    14041 atttgcagat gcggtgaaag tgcccgtgtt ggcgaacatt accgagtttg gttccactcc
    14101 gctttatacc caaagcgagc tggctgaaaa cggcgtgtcg ctggtgctgt atccgctgtc
    14161 atcgttccgt gcagcaagca aagccgctct gaatgtttac gaagcgatta tgcgcgatgg
    14221 cactcaggcg gcggtggtgg acagtatgca aacccgtgcc gagctgtacg agcatctgaa
    14281 ctatcatgcc ttcgagcaaa aactggataa attgtttcaa aaatgattta ccgctttcag
    14341 acggccttcc aatctatccg catcggccgt ctgaaaattg aaaatccata aaaaacaaag
    14401 gagaaatacg atgactgaaa ctactcaaac cccgactttc aaacctaaaa aatccgttgc
    14461 gctttccggc gttgcggcgg gtaatacagc attgtgtacc gtcggccgta ccggcaacga
    14521 tttgagctat cgcggttacg acatcttgga tttggcacaa aaatgcgagt ttgaagaagt
    14581 cgcccacctg ctgattcacg gtcatttgcc caacaaattc gagctggccg cttataaagc
    14641 caagctcaaa tccatgcgcg gcctgcctat ccgtgtgatt aaagttttgg aaagcctacc
    14701 tgcacatacc catccgatgg atgtgatgcg taccggcgta tccatgctgg gctgcgttca
    14761 tcctgaacgt gaaagccatc cggaaagcga agcgcgcgac atcgccgaca aactgatcgc
    14821 cagcctcggc agcatcctct tgtactggta tcaatattcg cacaacggca aacgcattga
    14881 ggttgaaagc gacgaagaga ccatcggcgg tcatttcctg caactgttgc acggcaaacg
    14941 cccaagcgaa tcccacatca aagccatgca cgtttcactg attctgtatg ccgaacacga
    15001 gttcaacgct tctaccttca ccgcccgcgt gattgccggt acaggctctg atatgtactc
    15061 cagcattacc ggagcaatcg gcgcgttgaa aggtccgaaa cacggcggcg cgaacgaagt
    15121 ggcttacgat attcaaaaac gctaccgcaa tgccgacgaa gccgaagccg acatccgcga
    15181 acgcatcggc cgcaaagaaa tcgtgatcgg tttcggtcat ccggtgtaca ccatctccga
    15241 ccctcgcaac gttgtcatta aagaagtggc acgcggtttg agcaaagaaa ccggcgatat
    15301 gcgcctcttt gacattgccg aacgtttgga aagcgtgatg tgggaagaga aaaaaatgtt
    15361 cccgaatctg gactggttct ccgccgtttc ctatcaaaaa ttgggcgtac cgaccgctat
    15421 gttcacaccg ctgttcgtga tttctcgtac aaccggttgg agcgcacacg ttctcgagca
    15481 acgcaaagac ggcaaaatca tccgtccgag cgcgaactac acgggccctg aagatttggc
    15541 gtttgtggag attgaagacc gctaatacgc gtaacagcag ggtgggccgc cagcccgccc
    15601 catctcgaaa tgccgtttga aaacaatgaa taaatgatgt tttcgttttt cgatggccta
    15661 gtgcagtatg cgagagagag ccggccgttt ggaaaacatg ctgctctgct gtcgtcattc
    15721 ccatacaggc agaaattcaa aagttgaaat gttctgctgt aaagagtgct gaaagattta
    15781 agtttggatt tccgtctata cgggaatgat ggtagcggag cagcaatttt tttcataccg
    15841 cgaatagatt tcagataaca agcaataaaa cagaatttat ggtttcagac ggcctgaagg
    15901 tccgaatcaa caggccgtct gaaaaaagaa atttaatgaa tttattggca tactaattat
    15961 taatgcggaa catttgatgg ataaggacaa ggggtcttat gctggtattc ccgtacaaaa
    16021 taccaaagag ttagtttggc agaaaataca agaaatacct atttctgaat gtggggaacc
    16081 gttggtcagt gttggtaata ccggacgcat tcgtgtcaat cctgtttatt tttcccaggg
    16141 tattgaaggt gcaataaata ctgttttatt gcgtagaagt gttgctgata ggctgactca
    16201 ggttgtctct ttgctgccag aaatgtacgg attggaaatt tttgatggtt ggcgtcctgt
    16261 catagtgcaa agagcattac gagaaaatat gaagcaagaa attgaaaaac gccatccgga
    16321 atatgatgaa gttcaagtta gagaagcact ggactgtttt gctgctgatc cagatcgggc
    16381 aggaatgcct ctgccccatt tgacaggcgg atctgttgat gtcggattgt ttgatataaa
    16441 aagtggcaaa agtttggata tgggaagtga gtttgatgca accggacatt tatcttatag
    16501 cgatgcttta gagaaagaag gtgagtattt ctcctctgct cgtaatttac gccggatttt
    16561 gattcatgcg atgaaagagg caggatttag taatttgcca accgaatggt ggcatttcga
    16621 ttatggcaac caaaattggg cttattttac ccaacaaagc aacgcagttt ttggggcggt
    16681 agaagagcag gatacttaaa ttaaaataaa aattgggagg aaaaatgcaa actttaattt
    16741 cagcagataa tcatttgctg ctgtgggctt ttgttatgtt agctgccgct gctgcgattt
    16801 ttattgagca aaacagtcgc ttggctggga aagtccctgg tgcagtatta gctttattga
    16861 ttgcgttgat tgcttctaat ttgggtgtta ttccgacgga tgctccagta tttgatgctg
    16921 tatggtctta tattgtacct cttgctattc cgttgttatt gtttcaatta gatcttcatg
    16981 ctctatttaa ggaaagtgga cgtatgctgt ttattttcct gattagttca ctagggacgg
    17041 tcattggaac gcttgtctca tttgcactat tgaaaaacta tattcctgaa ctagataagg
    17101 tagctggcat gatttcagca tcttatacgg gaggtggtgt taattttgca gctatgtcag
    17161 ctaagcttaa ccctgctaaa gatgttattg cagcaacaat tgttgctgat aatttgatga
    17221 tggcttgtta cttttttatt ttgattggta tctcttcctc atattgggtc agaaaagttt
    17281 ggggaagtcc atatcaggat aaattggagc aggaatatca tgatccgagt gtgaataagg
    17341 ctgccgagta ttggaaacct aaatccattt cgctacagag tatagcctta tctttggctg
    17401 cctcgatttt gttggtcgca gtttcattct cattatccag ttttttgcaa aatcattttc
    17461 aaagtgttga tggtgttgca ttaaaaatgc tgacaggttt aattagtgac aaatatttgc
    17521 tgttaacgac atttacattg gcagttgttt tatttttccg taaaggcatt caaaaactag
    17581 atggcagtca agaattaggg acatattgta tttatctgtt ttttgttgtg attggtgttc
    17641 ctgcctctat accgttaatt attaaaacag caccattact ctttgttttt actttgatta
    17701 ttgcggtatt gaatttgtta ctgacgttag tgttaggcaa gctgttcaaa ttcagtattg
    17761 aagaaattgt attggcttgt aatgccaata ttggagggcc gactactgca gcagccttag
    17821 ccatcagtaa aggttggcgt gatttagtcg gtgcaatttt gttaattggt actgtaggct
    17881 acattatcgg taactatata ggttcgtttg tatatgccct attgagctga agactaatct
    17941 aacaatccat gaaccttttc agacgacccc cttcaaaaaa cgtcgtctga aaccccaaac
    18001 cacaagaaaa atagatccac aggagaactg aacatggctg ccaaccaacg ttaccgcaaa
    18061 ccgctacccg gtacggattt ggaatactac gacgcgcgtg cggcgtgtga ggacatcaaa
    18121 cccggctctt acgacaagct gccttataca agccgtatct tggctgagaa cttggtcaac
    18181 cgcgcagaca aagtcgacct gccgacgctg caaagctggc tgggccagtt gatagaaggg
    18241 aagcaggaaa tcgactttcc gtggtatccc gcgcgggtgg tgtgccacga tattctgggg
    18301 cagaccgcgc tggtggattt ggcgggcttg cgcgatgcga ttgccgaaaa aggcggcgat
    18361 cctgccaaag tgaatccggt ggtgcaaacc cagcttatcg tcgaccactc tctggcggtg
    18421 gagtgcggcg gttacgatcc tgatgccttc cgcaaaaacc gcgaaatcga agaccggcgc
    18481 aacgaagacc gtttccactt catcaactgg acaaaaaccg catttgaaaa tgtggacgtg
    18541 attccggcgg gcaacggcat tatgcaccaa atcaatctgg aaaaaatgtc gcccgtcgtc
    18601 caagtcaaaa acggcgtggc tttccccgat acctgcgtcg gcacggattc gcacacgcca
    18661 cacgtcgatg cgctgggcgt gatttccgta ggcgtgggcg gtttggaagc cgaaaccgta
    18721 atgttaggcc gtgcatccat gatgcgtctg cctgatattg tcggcgttga gttgactggc
    18781 aaacgtcaac ctggcattac tgccaccgac atcgtgttgg cactgaccga attcttgcgt
    18841 aaagagcgcg tggttggcgc atttgtcgaa ttcttcggcg agggcgcaag aagcctgtct
    18901 atcggcgacc gagccactat ttccaacatg acccctgagt tcggcgcgac tgccgcgatg
    18961 ttcgctattg atgagcaaac cattgattat ttgaaactga caggccgcga cgacgcgcaa
    19021 gtgaaattgg tggaaaccta cgccaaaacc gcaggcttgt gggcagatgc cttgaaaacc
    19081 gccgtttatc ctcgcgtatt gaaatttgat ttaagcagcg taacgcgcaa catggcaggc
    19141 ccaagtaacc cgcatgcccg ttttgcgact gccgatttgg ccggcaaagg cttggctaaa
    19201 ccttatgaag agccttcaga cggccaaatg cctgacggtg cagtgattat tgccgcgatt
    19261 acttcctgta ccaatacttc caatccgcgc aacgttgtcg ccgccgcgct gttggcacgc
    19321 aatgccaacc gcctcggctt gcaacgtaaa ccttgggtga aatcttcgtt tgccccgggt
    19381 tcaaaagtag ccgaaatcta tttgaaagaa gcaggcctac tgcctgaaat ggaaaaactc
    19441 ggcttcggta tcgttgcctt cgcatgtacc acctgtaacg gcatgagcgg tgcgctggat
    19501 ccgaaaatcc aaaaagaaat catcgaccgc gatttgtacg ccaccgccgt attatcaggc
    19561 aaccgcaact tcgacggccg tatccacccg tatgcgaaac aggctttcct cgcttcgcct
    19621 ccgttggtcg ttgcctacgc gctggcaggc agtatccgtt tcgatattga aaacgacgta
    19681 ctcggcgttg cagacggcaa ggaaatccgc ctgaaagaca tttggcctgc cgatgaagaa
    19741 atcgatgccg tcgttgccga atatgtgaaa ccgcagcagt tccgcgatgt gtatgtaccg
    19801 atgttcgaca ccggcacagc gcaaaaagca cccagtccgc tgtacgattg gcgtccgatg
    19861 tccacctaca tccgccgtcc gccttactgg gaaggcgcgc tggcagggga acgcacatta
    19921 agaggtatgc gtccgctggc gattttgccc gacaacatca ccaccgacca cctctcgccg
    19981 tccaatgcga ttttggccgt cagtgccgca ggcgagtatt tggcgaaaat gggtttgcct
    20041 gaagaagact tcaactctta cgcaacccac cgcggcgacc acttgaccgc ccaacgcgct
    20101 accttcgcca atccgaaact gtttaacgaa atggtgaaaa acgaagacgg cagcgtgcgc
    20161 caaggctcgt tcgctcgcgt cgaacccgaa ggcgaaacca tgcgcatgtg ggaagccatc
    20221 gaaacctata tgaaccgcaa acagccgctc atcatcattg ccggtgcgga ctacggtcaa
    20281 ggctcaagcc gcgactgggc tgcaaaaggc gtacgcctcg ccggcgtgga agcgattgtt
    20341 gccgaaggct tcgagcgtat ccaccgcacc aacttgatcg gtatgggcgt gttgccgttg
    20401 cagttcaaac cgggtaccaa ccgccatacc ctgcaactgg acggtacgga aacctacgac
    20461 gtggtcggcg aacgtacacc gcgctgcgac ctgaccctcg tgattcaccg taaaaacggc
    20521 gaaaccgttg aagttcccgt tacctgccgc ctcgataccg cagaggaagt gctggtgtac
    20581 gaagccggcg gcgtgctgca acggtttgca caagactttt tagaaggtgt ggtagcataa
    20641 aggtagacgg tcttttttat gatttatgag aagaccgttt gaaaattatt tatggttaaa
    20701 tttttaattt aaattaaaca gtaaatatga agacacaaga agatgaggta actaagcaga
    20761 aaatttttaa tcatatgatt aataataaat catttttaaa tttccagttt tggacatttg
    20821 ctgctttgag tgcaggttca tttgcctttc taataagtgc agcaaatata gattctaaag
    20881 atagagactt gacgcttgaa atagccattg ttggatattc agtaagttta atattaaatt
    20941 ccatactatg ttttttaata agaggcatag aagacgatga gttatcggtt aaaatgttaa
    21001 atgaatttaa tttggataag cgtacttttt ggattcccat tctagcaacg tggagttttg
    21061 cgctttcgac acttttctta ttgtttacca ttagtctagt aggagcaatt actgctctta
    21121 taatggctgt tttagcaata attagtttta taaaatttac aaaaaaatca tagattttta
    21181 caatttgaag tggtgcgtaa atcgggcatt gatgcccgac acagaagttg taggttgagt
    21241 taagccccgg cattttgcta aatccaacgg ttttttcggg tttgcaaccc aacctacctc
    21301 tgctgctaat ctgcatcata caaaggagaa ctcgaaatgc cgcaaattaa aatccccgcc
    21361 gtttactacc gcggcggtac atctaaaggc attttcttca aacgcaccga cctgcccgaa
    21421 gcggcgcaac aagcaggcga ggcacgcgac aaaatcctgt tgcgcgtact cggcagcccg
    21481 gatccctacg gcaagcagat agacggtttg ggcaacgcca gttcgtccac cagcaaagcc
    21541 gtgattttgg acaagtccga acgcgccgat cacgatgtcg attacctttt cgggcaagtt
    21601 tccatcgaca aaccttttgt cgattggagc ggcaactgcg gcaacctcac cgctgccgtg
    21661 ggcgcattct ccatcgaaca gggcttggtc gataaaggca agattccttc agacggcatc
    21721 tgcacggtca aaatctggca gaaaaacatc ggcaaaacca ttattgccca tgtaccgatg
    21781 caaaacggcg cagttttgga aacaggcgat tttgagctcg acggcgtaac gttcccggca
    21841 gccgaagtac aaatcgaatt tcttgatcca gccgacggcg aaggcagtat gttcccaacc
    21901 ggcaatttgg tcgatgaaat tgatgtgccg aatataggcc gtttgaaagc cacgctcatc
    21961 aacgcgggca ttccgaccgt tttcctgaat gccgccgact tgggctacac gggcaaagag
    22021 ttgcaagacg acatcaacaa cgatgccgca gctttggaaa aattcgagaa aatccgcgct
    22081 tacggtgcgc tgaaaatggg tctaatcagc gacgtatccg aagctgccgc ccgcgcgcac
    22141 acgccgaaag tcgccttcgt cgcgcccgcc gccgattaca ccgcctccag tggcaaaacc
    22201 gtgaatgccg ccgacatcga tttgctggta cgcgccctga gcatgggcaa attgcaccac
    22261 gcgatgatgg gtaccgcctc tgttgccatt gcgaccgccg ccgccgtgcc cggtacgctg
    22321 gtcaaccttg ccgcaggcgg cggaacgcgt aaagaagtgc gcttcgggca tccttccggc
    22381 acattgcgcg tcggtgcagc cgccgaatgt caggacggac aatggacggc caccaaagcg
    22441 gttatgagcc gcagcgcacg cgtgatgatg gaaggttggg tcagggtgcc tgaggattgt
    22501 ttttaaattg acgtagcatg ggtttgcccg cgagccataa aaaggtcgtc tgaaaaacaa
    22561 gtaaacatca aatcactgac cattcccttc ccttgccctg tggcggaagg cggcaaatca
    22621 caaggaagaa cacggaaacc ccgataaaag acagcttccc gtattaccgt cattcccgcg
    22681 caggcgggaa tccagacctg tcaatatgga ggattggtag gggaaaacag gtttcgtgag
    22741 ttctacattc tggattcccg ccacagcctg tcctcgcgta ggcggggacg gaataacgat
    22801 agaaaatgcg gcatacgctt tgcccaaaga ggccgtctga aacaccttgc gcctgatgtc
    22861 tgcctttttc agacgacccc acaccaaaaa aacaaccaca aactacaagg agaaacatca
    22921 tgtccgacca actcatcctc gttctgaact gcggcagttc atcgctcaaa ggcgccgtta
    22981 tcgaccgaaa aagcggcagc gtcgtcctaa gctgcctcgg cgaacgcctg accacgcccg
    23041 aagccgtcat tacgttcaac aaagacggca acaaacgcca agttcccctg agcggccgaa
    23101 attgccacgc cggcgcggtg ggtatgcttt tgaacgaact ggaaaaacac ggtctgcacg
    23161 accgcatcaa agccatcggc caccgcatcg cccacggcgg cgaaaaatac agcgagtctg
    23221 ttttgatcga ccaggccgta atggacgaac tcaatgcctg cattccgctt gcgccgctgc
    23281 acaaccccgc caacatcagc ggcatccttg ccgcacagga acatttcccc ggtctgccca
    23341 atgtcggcgt gatggatact tcgttccacc aaaccatgcc ggagcgtgcc tacacttatg
    23401 ccgtgccgcg cgagttgcgt aaaaaatacg ctttccgccg ctacggtttc cacggcacca
    23461 gtatgcgtta cgttgcccct gaagccgcac gcatcttggg caaacctttg gaagacatcc
    23521 gcatgattat tgcccactta ggcaacggcg catccattac cgccatcaaa aacggcaaat
    23581 ccgtcgatac cagtatgggt ttcacgccga tcgaaggttt ggtaatgggt acacgttgcg
    23641 gcgacatcga tccgggcgta tacagctatc tgacttccca cgccgggatg gatgttgccc
    23701 aagtggatga aatgctgaac aaaaaatcag gtttgctcgg tatttccgaa ctttccaacg
    23761 actgccgcac cctcgaaatc gccgccgacg aaggccacga aggcgcgcgc ctcgccctcg
    23821 aagtcatgac ctaccgcctc gccaaataca tcgcttcgat ggctgtgggc tgcggcggcg
    23881 ttgacgcact cgtgttcacc ggcggtatcg gcgaaaactc gcgtaatatc cgtgccaaaa
    23941 ccgtttccta tcttgatttc ttgggtctgc acatcgacac caaagccaat atggaaaaac
    24001 gctacggcaa ttcgggcatt atcagcccga ccgattcttc tccggctgtt ttggttgtcc
    24061 cgaccaatga agaactgatg attgcctgcg acactgccga acttgccggc atcttgtagc
    24121 caaaaaaggg acgagtccgc aaaaatgccg tctgaaattt tcagacggca ttttgtttgg
    24181 attttgggcg tacaatacgg aaaacatgac gataaggaca caaactatgg cacagttttt
    24241 cgccgttcat cccgacaacc cccaagaacg cctcatcaag caggcggtcg aaatcgtcaa
    24301 taaaggcggc gtggtcgttt atccgaccga ttcctgttat gccttgggct gcaaactcgg
    24361 cgataaggcg gcgatggaac gcatactctc catccgcaaa atcgatttga aacaccacct
    24421 gaccctgatg tgcgcagatt tgagcgagtt gggcacatac gccaaagtcg acaacgtaca
    24481 gtttcgtcag cttaaagccg ccacacccgg gccttatact tttattttac aggcgacgaa
    24541 ggatgtgccg gcgcgcacgc tgcacccgaa acgcaaaacc atcgggctgc gtattcccga
    24601 taatgccatt gcacaagccc tgctggggga attgggcgag ccgcttttaa gctgcaccct
    24661 gatgctgccc gaagacggcg aaccattgac cgatccttat gaaatccgcg agcgtttgga
    24721 acacgccgtc gatttggtga ttgacggcgg ctggtgcgga accgagccga ccaccgtcgt
    24781 cgatatgacc gacggcacgg aattggtgcg ccaaggttgc ggcgatacgg cggtgttcgg
    24841 tttgtaggga aaccgatgcc gtctgaagca tcggctgttc agacggcatt gcgcgccttg
    24901 ccggcggcag tccgaaatgc cggcgcgtat cgcgctcggt cggaatatcc gtttgaaacg
    24961 gcattttgat gcattactgc accgcaatcg gaattctcgg ttcgtagagc aggtcgtagg
    25021 tcggcttgtt gagcaggtct tggagcgtga aaccgtccag atacgtgaaa aacgacttca
    25081 tcgcgccgcc gagtatgccc gtcagccggc aggacggtgt aatcaggcat tcgttgttct
    25141 cgcccatgca ctcgaccagc tgcatcggtt cgaggtggcg gacaaccgag ccgatgttga
    25201 tgcggtcggg cggtgcggca agccgcagac cgccgccttt tccgcgcaca ctgtggagga
    25261 agccgccttt gaccagcgcg gtaacgacct tcatcagatg gcttttggaa atgccgtagg
    25321 ttacggcgat ggtactgatg ttgaccagcg catcgtcgtt gatggcagtg tagataagga
    25381 cgcgcagccc gtagtccgta tgttgtgtca aatacatgat tttctcggta tggattgtta
    25441 ttcttatcgg tacggtttaa ggttcacgga caatacctta atggttgaaa ccctgtccgt
    25501 cggggcggta gaatgcagcc tgtctgcggc ggtatgccgt ctgaaacatc cgcgctaccg
    25561 tttgagaatt tgttattgta actcaaaatc atgaaaccgt tgaaacgaca tcccgccctt
    25621 atcgggcttt cgcgtgacca ccaccattcg ctttccctgt gcgtgcgtct gttgcggacg
    25681 ccggaagaaa ggcatcggga cgaactcgaa ccgcattttt ccgaattgga aacccatttt
    25741 cgcgaagaag aagccaagtt tgccccaatt tggcagaatg tcgcccccga attgaaacaa
    25801 cgtttcgaga aagaccacgc ccgactgcgg cagatgatgg caagccccga atacggtaac
    25861 gcggcgtgga ataccgcttt tgccacaacc ctgcgcgacc acgcgcgctt tgaagaacgc
    25921 gagctgtttc ccgccgccga accgtttttg ccggcatgat tccgttttgc ggcaaatata
    25981 ttaatgataa acaaggaaca cacatgaaat ttaccaagca ccccgtctgg gcaatggcgt
    26041 tccgcccatt ttattcgctg gcggctctgt acggcgcatt gtccgtattg ctgtggggtt
    26101 tcggctacac gggaacgcat gagctgtccg gtttctattg gcacgcgcat gagatgattt
    26161 ggggttatgc cggactggtc gtcgtcgcct tcctgctgac cgccgtcgcc acttggacgg
    26221 ggcagccgcc cacgcggggc ggcgttctgg tcggcttgac tatcttttgg ctggctgcgc
    26281 ggattgccgc ctttatcccg ggttggggtg cgtcggcaag cggcatactc ggtacgctgt
    26341 ttttctggta cggcgcggtg tgcatggctt tgcccgttat ccgttcacag aatcaacgca
    26401 actatgttgc cgtgttcgcg ctgttcgtct tgggcggcac gcacgcggcg ttccacgtcc
    26461 agctgcacaa cggcaaccta ggcggactct tgagcggatt gcagtcgggc ttggtgatgg
    26521 tgtcgggttt tatcggtctg attggtacgc ggattatttc gttttttacg tccaaacggt
    26581 tgaatgtgcc gcagattccc agtccgaaat gggtggcgca ggcttcgctg tggctgccca
    26641 tgctgaccgc catgctgatg gcgcacggcg tgatgccttg gctgtcggcg gctttcgcgt
    26701 ttgcggcagg tgtgattttt accgtgcagg tgtaccgctg gtggtataaa cccgtgttga
    26761 aagagccgat gctgtggatt ctgtttgccg gctatctgtt taccggattg gggctgattg
    26821 cggtcggcgc gtcttatttc aaacccgctt tcctcaatct gggtgtgcat ctgatcgggg
    26881 tcggcggtat cggcgtgctg actttgggca tgatggcgcg taccgcgctc ggtcatacgg
    26941 gcaatccgat ttatccgccg cccaaagccg ttcccgttgc gttttggctg atgatggcgg
    27001 caaccgccgt ccgtatggtt gccgtatttt cttccggcac tgcctacacg cacagcatac
    27061 gcacctcttc ggttttgttt gcactcgcgc ttttggtgta tgcgtggaag tatattcctt
    27121 ggctgattcg tccgcgttcg gacggcaggc ccggttgaga caaaccgccg cagatttcgg
    27181 gtctgggcgg tttgcttttc agacggcagg gcggtcagtt gccgtccagc cagcggtcgc
    27241 gtgtggtttt ggcttcttca aaatagcggt acagggcttc gcggtcgtcg gtggtcagga
    27301 tgtttgccaa aacgtccaac tgtttgccca agccttgaac cagttgcagc aggctgtctt
    27361 tgttggcaag gcagatgtcc gcccacacgg cgggatgacc ggaggcgatg cgggtgaagt
    27421 cccgaaagcc cgtggcggcg aatttcagat attcctgtcc gtcggggtgg tcgagaatct
    27481 ggtggacata ggcgaaggcg gtcaggtggg gcatatggga gacggcagcg aaaaccgcgt
    27541 cgtggcgttg cgcgtccatc gtataaattt ccgcaccgac cgcgtgccac agttcttcta
    27601 ccaaggcaat gccgtctgaa tgttcgccgc cgtgtggcgt gatgatgagt tttctgtggc
    27661 ggaacagccc gaactgcgcg gcttgcgcac cgcttctgtc cgaaccggca atcgggtggg
    27721 cggcgatgca gcggtgcagg cggtcgggca gacagcggcg gaaggcttcg atgaccgaag
    27781 atttggtgct gccgacatcg gaaatccaag tgtgttccgg caaaacgggg cgcagcgcgg
    27841 tcaaaatggc gggaaaggtg gcgacgggcg tggcaatcag taccaagtcc gcaccgccga
    27901 tgctgtccgc gtcgatggca acggaggcac ggtcaatcac gccgcgttcc aatgcacgtt
    27961 cgaggttgtc gcggtcggtg tcgataccgg taacggtgcg gacgagtccc tgccttttga
    28021 ggtcgagaac gaacgaaccg ccgatcagcc ctacaccgat gagggcaata tggttcaaaa
    28081 tgggcatttg tgtaaacggt tttcgcaaag taccgtcatg gtagcctatc ggcggaatat
    28141 gccgcaaggt cggcaggaaa aaggagaaga aatggacaaa atcagagttg ccgccgtgca
    28201 gatggtgtcg ggcgtgtcgc cggaaaccaa cgtcgctgcc atgaaacgcc tggtcgcacg
    28261 ggcggcggag cagggtgtgg attgggtgct gctgcccgaa tattgggtgc tgatgggcgc
    28321 aaacgatacc gacaaactcg cgcttgccga gcctttgggc ggcggacgct ttcagacggc
    28381 attgagcgaa acggcgaaag aatgcggcgt ggtgctgttt ggcggaaccg tgccgctgca
    28441 aagccccgag gcgggcaagg tcatgaatac tttattggtg tacgggcgcg acggcaaacg
    28501 gacggggctg taccacaaaa tgcacctctt cggcttttcc ggtttgggcg aacgctatgc
    28561 cgaagccgat accatccgcg cgggcgggga agtgccgcac ttgtcggcag agggcgtgcc
    28621 ggtggcagca ggtatttgtt acgatgtccg ctttcccgaa tttttccgcc gccagctgcc
    28681 gtttgacgta ttgatgctgc ccgctgcgtt tacgcacacg acgggcaagg cgcattggga
    28741 gctgctgctg cgcgcgcgtg ccgtcgaaaa ccaatgttac gtcgtggcgg cggcacaggg
    28801 cggtttgcac gaaaacggac ggcgcacgtt cggacacagc atgattgtcg atccgtgggg
    28861 cgacgtgttg gacgtattgc ccgaaggcga aggcgttgtt acggcagaca tcgatgccaa
    28921 ccgcctgaac agcgtccgca accgcctgcc cgccttgaaa cactgggttt tggatgccgt
    28981 ctgaaggttt caaacggcat cggtgtcggg gaatcagcgg gctgtaggat gggcttcagc
    29041 ctgccatttc catcaatccc acatttctac cgttttcatc gaatccatcg aatccgcccc
    29101 tttcgacgac ccagtcc
//
LOCUS       DAXHPG010000006        26950 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXHPG010000006 DAXHPG010000000
VERSION     DAXHPG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26950
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.006"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(201..488)
                     /locus_tag="ACFM6F_000183"
     CDS             complement(201..488)
                     /locus_tag="ACFM6F_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163684.1"
                     /translation="MDDLIAAFLGYLSGKYNEGKISRLGISLTAGSIFFAVFFTQELI
                     IFIIDGNKENLWNFITGIMKDLFLFSCSVCLLFYFLLFIAETIRKTINKIK"
     gene            complement(691..906)
                     /locus_tag="ACFM6F_000184"
     CDS             complement(691..906)
                     /locus_tag="ACFM6F_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163685.1"
                     /translation="MVNKYIKTAISITAISIFYLLTQESSGKTEEPSYFLMFMFLNSL
                     WFEENKTVMAAVTAMIAAHLIFVTLSD"
     gene            complement(896..1291)
                     /locus_tag="ACFM6F_000185"
     CDS             complement(896..1291)
                     /locus_tag="ACFM6F_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163686.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAWGPVGAFIGGVGGGIGYMMNQQLSGSRFSPTAFTLAVGAGAFGGA
                     TAGPAGVVWGFNRSLATGMVTGVAQRYGK"
     gene            complement(1454..1727)
                     /locus_tag="ACFM6F_000186"
                     /pseudo
     CDS             complement(1454..1727)
                     /locus_tag="ACFM6F_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2289..2762)
                     /locus_tag="ACFM6F_000187"
     CDS             complement(2289..2762)
                     /locus_tag="ACFM6F_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163687.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFASTRSTLAATAGGMI
                     GGGYSSAMLRGAGITTSVFARSAWKGGNAIPNAVIRANGAALNQSSSAAMGKHRR"
     gene            complement(2805..3674)
                     /locus_tag="ACFM6F_000188"
     CDS             complement(2805..3674)
                     /locus_tag="ACFM6F_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC6163688.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAALVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNSKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGKQPDRIDGKKESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKLGVQHTF"
     gene            3838..4761
                     /locus_tag="ACFM6F_000189"
     CDS             3838..4761
                     /locus_tag="ACFM6F_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6163689.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(4839..5480)
                     /locus_tag="ACFM6F_000190"
     CDS             complement(4839..5480)
                     /locus_tag="ACFM6F_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6163690.1"
                     /translation="MQKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGHGETQDCIAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(5535..6326)
                     /locus_tag="ACFM6F_000191"
     CDS             complement(5535..6326)
                     /locus_tag="ACFM6F_000191"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC6163691.1"
                     /translation="MARHPYRRLRPAKSSFPEVGISEEGNIRSLHLGSDTIQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARSLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLADLKASNPNNGKHFHL"
     gene            6492..6567
                     /locus_tag="ACFM6F_000192"
     tRNA            6492..6567
                     /locus_tag="ACFM6F_000192"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6525..6527,aa:Thr,seq:tgt)
     gene            6602..6677
                     /locus_tag="ACFM6F_000193"
     tRNA            6602..6677
                     /locus_tag="ACFM6F_000193"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6635..6637,aa:Thr,seq:tgt)
     gene            6819..7610
                     /gene="panB"
                     /locus_tag="ACFM6F_000194"
     CDS             6819..7610
                     /gene="panB"
                     /locus_tag="ACFM6F_000194"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC6163692.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVLAELAKKVTETVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            7733..8569
                     /gene="panC"
                     /locus_tag="ACFM6F_000195"
     CDS             7733..8569
                     /gene="panC"
                     /locus_tag="ACFM6F_000195"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC6163693.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYCELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            8734..10578
                     /locus_tag="ACFM6F_000196"
     CDS             8734..10578
                     /locus_tag="ACFM6F_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6163694.1"
                     /translation="MLPNRFKMLTVLTAALIAGQVSAAGSGAGDMKQPKEVGKVLRKH
                     QRYSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPE
                     VAERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLE
                     EVLAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSV
                     QGREKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQ
                     EMEIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAY
                     GRGTEEQRSRAALTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGG
                     RAALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGS
                     NTELQAEALVQRSVVYDRLGKRKKMISDLERAFRFAPDNAQIMNNLGYSLLSDSKRLD
                     EGFALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGE
                     VLWALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            10719..11300
                     /locus_tag="ACFM6F_000197"
     CDS             10719..11300
                     /locus_tag="ACFM6F_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC6163695.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETLERCAARTR"
     gene            11310..12155
                     /gene="ispE"
                     /locus_tag="ACFM6F_000198"
     CDS             11310..12155
                     /gene="ispE"
                     /locus_tag="ACFM6F_000198"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC6163696.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKLRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCSRTTQQLIDLGATLGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRDSAYNIYRQVSDLYEAYLAEGLSKH
                     PWLSV"
     gene            12163..12238
                     /locus_tag="ACFM6F_000199"
     tRNA            12163..12238
                     /locus_tag="ACFM6F_000199"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12196..12198,aa:Gln,seq:ttg)
     gene            12251..12326
                     /locus_tag="ACFM6F_000200"
     tRNA            12251..12326
                     /locus_tag="ACFM6F_000200"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12284..12286,aa:Gln,seq:ttg)
     gene            12335..12410
                     /locus_tag="ACFM6F_000201"
     tRNA            12335..12410
                     /locus_tag="ACFM6F_000201"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12368..12370,aa:Gln,seq:ttg)
     gene            12577..13560
                     /locus_tag="ACFM6F_000202"
     CDS             12577..13560
                     /locus_tag="ACFM6F_000202"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC6163697.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            13627..14199
                     /locus_tag="ACFM6F_000203"
     CDS             13627..14199
                     /locus_tag="ACFM6F_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC6163698.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(14626..14844)
                     /locus_tag="ACFM6F_000204"
     CDS             complement(14626..14844)
                     /locus_tag="ACFM6F_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163699.1"
                     /translation="MRAVEICGFFFAGDEGAGSGALQVEIGGGFARPIELITFARTGK
                     VFCAEDEFELFKVNLVFTKDFGKELFEF"
     gene            14848..16476
                     /locus_tag="ACFM6F_000205"
     CDS             14848..16476
                     /locus_tag="ACFM6F_000205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6163700.1"
                     /translation="MFIEAENLEALKILQKSYAGSVKMIYIDPPYNTGNDSFVYPDKF
                     SESREEYARRVGDTDDAGYLKRDGVFQGAWRKNGKDSGHYHSNWLSMMLPRLHLAKTL
                     LREDGVIFISIDDNEQAQLKLLCDEVFGAENFVGQFPWRKRTAKSDVPFGVSADYEYL
                     LAYARSNAFIASIDAEERRYFETDDFPNRPWRTHDLTTQRNASERPNSFFTMVNPKTG
                     KEYPANPNATWRVTKDTFQEYYNAGKIVFPDDYDFLKIKNPVMRYFKDDDMKKDGERF
                     GRKAVSTRLPENVGMSLNGTKEITELFKSKLFDFPKPSSLIKYLLQILHVSDGLILDF
                     FSGSGTTAHAVMQLNAEDGGNRRFICVQLPEETDEKSEARKAGFDTIAEIAKERIRRA
                     GRQISDGLQDGSETDTGFKVFKLAESGFKQWRQPEQSDTEALQRELSLNIDSVLSETS
                     SENLLYELMLRMGLKLTCKVSFLDDVYFVEDEDTGGLYAFLLKRVDQGLIDAVLAKHP
                     AKVAALDRLFEGDDALKSNTVLQMKDAGVMFECV"
     gene            16711..19362
                     /locus_tag="ACFM6F_000206"
     CDS             16711..19362
                     /locus_tag="ACFM6F_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC6163701.1"
                     /translation="MELKFEELPYQLDAVNAVANLFAGQPNHARTFDLTSQGTGRFVG
                     NGLDLDWETLGLNLNTVQKQNGQPETEIGAHGLNFSLEMETGTGKTYVYLRTIYELNR
                     SYGWKKFVIVVPSVPIREGVLKSLEITKAHFDGVFGRPVMRYAEYSSSRLSDLRTFAV
                     NDHIEILVINIQSFEKDGNVINKVNESGETPIKFIQETCPIVIIDEPQNMETEGRLNA
                     LESLNPLFTLRYSATHKQPYHKVYSLNPVQAYNLKLVKQIEVLPVLAENDVNGAFVEL
                     LEVKQAAKGRPKADLNIHYQDKKETKKKKVSVKWEDDLFEKSGGNEAYRHGFIIDGMD
                     FDKQELAFSSGKTITRGGDGDAVRDEIMKNQIRETVREHLAKEKRLNPLGIKVLSLFF
                     IDRVENYRTEDGTAGKFALWFEEIYRELAGNTDTAGVHDGYFSQDRKGRLKNTDGTTQ
                     ADNDTYRLIMRDKETLLSFDSPLRFIFSHSALKEGWDNPNVFQICTLNETRSPLKKRQ
                     EIGRGLRIAVNQEGRRVRDENVNILTVVPNESYELFAANLQKEYEDECGIKFAAENIK
                     NGNKKKKQLFRKDFPLDPEFQAIWDKLKYKTRYRVRFDTEELIKQASEAVANLPEIQK
                     PKIRIRKAQLKQSQTYGIETVEIAGRSREMDVQWEIPDILGEIQRKTGLTRRTVYEII
                     IQSGRLKDVAGNPQRFIDLTAEAVNRSLHALMAAGIEYERTGEVYDQKLLEDMAREGI
                     EFYENGFTFTVSNPQKTICENYIPLDSGTEKAFAQNCEDYDAKNEGGVAFYFKLPGWF
                     KIPTPLGNYNPDWAVVKQVRDKVYFVAETKNTGKGIQTGVDMDKLRQNERLKIKCGKR
                     HFEALGGLAYRVVEKVGDLKSEKEEQA"
     gene            complement(19524..20693)
                     /locus_tag="ACFM6F_000207"
     CDS             complement(19524..20693)
                     /locus_tag="ACFM6F_000207"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC6163702.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLVGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKTTEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTLAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            20841..22367
                     /gene="ilvA"
                     /locus_tag="ACFM6F_000208"
     CDS             20841..22367
                     /gene="ilvA"
                     /locus_tag="ACFM6F_000208"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC6163703.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(22424..23497)
                     /locus_tag="ACFM6F_000209"
     CDS             complement(22424..23497)
                     /locus_tag="ACFM6F_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC6163704.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(23494..24354)
                     /gene="cysW"
                     /locus_tag="ACFM6F_000210"
     CDS             complement(23494..24354)
                     /gene="cysW"
                     /locus_tag="ACFM6F_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC6163705.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGRLEAQGIQIIFAIPGIVLATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(24606..25442)
                     /gene="cysT"
                     /locus_tag="ACFM6F_000211"
     CDS             complement(24606..25442)
                     /gene="cysT"
                     /locus_tag="ACFM6F_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC6163706.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMLWALGRRSGA
                     KG"
     gene            25581..25688
                     /locus_tag="ACFM6F_000212"
                     /pseudo
     CDS             25581..25688
                     /locus_tag="ACFM6F_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            25733..26065
                     /locus_tag="ACFM6F_000213"
     CDS             25733..26065
                     /locus_tag="ACFM6F_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163707.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GEACACGH"
     gene            26396..26905
                     /locus_tag="ACFM6F_000214"
     CDS             26396..26905
                     /locus_tag="ACFM6F_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC6163708.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT         6818 a         6213 c         7232 g         6687 t
ORIGIN      
        1 tccactatac aaaacaatca agaaactatt ggcggtatgt ataatcttga attttgtttt
       61 ttgatgaaat taaaataatg aaatatatgg taaacgttac aacggaagcc ttagcctccc
      121 gattgcagta tttcaattcc aagataaatg ccgcatagaa aagataaaat acttaaatgg
      181 tttttagcgg cttaaatatc ttatttaatt ttattgattg ttttcctaat agtttctgca
      241 ataaataata agaaatagaa taataaacaa acagaacatg aaaataaaaa taaatctttc
      301 attatcccag taataaaatt ccaaagattt tccttatttc catcaataat aaaaattatc
      361 agttcttgag taaagaatac tgcaaagaaa atgcttcctg ccgttagaga tattcctaac
      421 ctgcttattt tcccttcatt gtatttacct gataaatagc ccaaaaaagc tgcaatcaaa
      481 tcatccattt taaaaatttc tgtaaaaaca gataatgtcc ttaattcatt ttgtccaatc
      541 cgtttgccat tgcggggcaa tcatagacag catcatcacg gctgcggtga tttgtttttt
      601 cataacaaat tcttagggag gagataatgt ttcatcaaag tcctcctgaa aggctgcctg
      661 aaatttccaa gcagcctttt tgtttgctta ttaatcggat agtgtgacaa atataaggtg
      721 ggcggcaatc attgccgtaa cggcagccat aactgtttta ttttcttcaa accaaagcga
      781 gttgagaaac atgaacatca agaaatatga tggttcttca gttttgccgc tgctttcttg
      841 agttagaaga taaaatatgg atatggcggt tatgctgatt gctgttttta tatatttatt
      901 taccatatcg ttgggcaaca cccgtaacca tacctgttgc caaagatcgg ttaaatcccc
      961 atacaacccc tgccggacct gctgtcgccc ctccaaaagc tcccgcccct acggcaagag
     1021 tgaatgctgt gggagaaaat ctgcttccgc taagttgctg gttcatcata tatccgattc
     1081 cgcctccgac tcctccgata aatgcaccta ccggtcccca agccccctca gtctccttca
     1141 tctccttttg agaaagctcc gccaactgca tcggcgcatc tgcctgcgtg tggaacactt
     1201 ggttttcaaa tgcctgattg tccaagccgt ttgccattgc gggggcaatc atagacagca
     1261 tcattacggc tgcggtgatt tgttttttca taataactcc tttggattac aaggttggaa
     1321 aatcaaagcc ctgcttagaa cgtatgttgc acacccaatt tcaataggta aatcagattg
     1381 caaatccagc aatttgaata ttgtcattgt tccgtgcaaa agggattttt attgatgagt
     1441 tgtgtactgg gtttcagctt ggctttttag ataatctttt cacaagaaca tacatcaata
     1501 ctgtccaaaa aaaaaagatt tgataaatat attgcaacat attttattcc acctccatcc
     1561 gaaacagaaa ccaatatatt tttaattaat acatagctga ttattacatt tattattcct
     1621 ataatatata aggatcttgc caaaattttt tttgatttta ttttaggaat atctcttatc
     1681 catgctaaaa tacagcccaa tgtcgaaaag aaaataacag caaacactac cttacctcct
     1741 ctcacttcca accaaaccga tagcaggttt ggttggaagt tggtttattt attatttaac
     1801 ggcgaatgtc agtgttctta cccgtagaac ctgcataacc atttatgcca ccagcaacag
     1861 tccccactgc tacacctcgc ataccattaa tcagaattct tcctattttt tcgaatccac
     1921 tatttcttcc aaagatgcag caaacgcagg ttgagcctag acccaatcta cgtttgctgc
     1981 cgcatctgat ccctattgtt tcttatcctt acatcttcct gccttgtcaa tcaaataaag
     2041 acagaagact atacaaaaac tgaccgacat actgaaaata cctatcccct tccatgcaat
     2101 ctccgcacca ttgacccaat agattagact gagaaacaac aaagccacag tataaatcaa
     2161 cgtaaaaaat attgcgtaaa gcacaataaa gggaactttt atctcacggt tgttttttat
     2221 aatatattca gcaacttgat ttccgaatat acctgataaa aaatatagta ttagatcatc
     2281 catttcgttt atcttctatg ttttcccatt gcggcgctag aagactgatt taatgctgca
     2341 ccatttgcac gaataactgc attaggaata gcatttcccc ctttccaagc agaacgggca
     2401 aaaacactag tagtaatccc tgcaccgcgc aacatcgcac tgctatatcc gccgcctatc
     2461 ataccaccag cggttgcagc caaggtactt cttgttgaag caaattgccc tgttttaatt
     2521 ttagaaatgc cgtgattagc ccaagcacta attgcccctc ccatcaaagc acctgccaca
     2581 ataggaagaa acgccccctc agtctccttc atctcctttt gagaaagctc cgccaactgc
     2641 atcggcgcat ctgcctgcgt gtggaacact tggttttcaa atgtctgatt gtccaagccg
     2701 tttgccattg cgggggcaat catagacagc atcattacgg ctgcggtgat ttgttttttc
     2761 ataacaactc ctttggatta caaggttgga aaatcaaaac ctgcttaaaa tgtatgctgt
     2821 acgcccaatt tcagttcgga actgctttgc cctgaaacgt tgaaacgtgc ggatgcgttt
     2881 aaagccgtgg ttttggtgaa accgaaacct gcgccgaaat gggcgtaggt ggatgtgttt
     2941 cttgcggatt cttttttgcc gtctatgcgg tcgggctgtt tgcccagcca ttggatgcct
     3001 ccggtcaggc tgattctgtc gttggcggca aatgagatgt tggggttgag cagcaggtag
     3061 ttgcccgatt tgtagcggat gccgtctgaa agggttttgc tgccgttgat gcggtaggcg
     3121 gcggtgaggg aaaggacaat cggatctatg gctttgtagg tggtggcgcc gatgagccac
     3181 gattttcccg acgaggcttt gttgcgcgat ttttcgtaaa ccgtgctttc aagaaagctg
     3241 attagggcgg ggtttttgtc gtctttaagg aaggtgtggc tgatgccgag ggatacgtcg
     3301 gacatccgtt tgttgcgggt tttgctgttg ccgtcgagtt tgcgttcttc gtgccacaga
     3361 tagctgccgc tgccgtaaat gtcggtattc ccggtcagtc cgtagcgcaa accgagcgtg
     3421 ccaacgagca tatcggtatt gctgccgttt tcttggattt cggtcggaat ggggataaac
     3481 gaggttgcgc cggtttggat gtaaaccagt gcggcaagtt cggcgcggtt gttttcgcta
     3541 ttcaggtagg taagggaagt ttccagtttc catttgccct tgtcagtcat tatgtcttca
     3601 atcgtcaagg gcaggtcggc ataagcggat aaaggcagga tggcgggcaa ggcgggcaaa
     3661 aagatgcgct tcatatttct cctattgata ttaattctta tttaagaata gtaatgaata
     3721 tccgataatt ttgtctaaat aaatttttaa atcaaaaaaa acgatatatt tgtttttatc
     3781 aattatgttt tgatatgccg tctgaaaagt ttgtgaaaaa cggttacaat ccgccgcatg
     3841 aaaaaacgca ataatcctct tccgttgttg aacggtgtca aacccagtta tttggtgctg
     3901 ccgcatgaaa agcagtttta cgggctgccg ctgctgcatt ttctgtgcat ccgctttcct
     3961 tttgtgggcg cggacgattg gcgcaggcgg ttgaacagcg gttttgtggt cggttcggat
     4021 ggtgcggcgt tggacgaaca tactttgttc gagccgggta aggtgatgtt ttattatcgg
     4081 gaaaccagca gtgaggatga gccgcgtatt ccgtttgaag aaaagatttt gcatattgat
     4141 gagcatttga ttgtggtgga caagccgcat tttctgcccg tcatccccag cgggcgtttt
     4201 ttaagggaaa cgctgctcac acgtctgcgc ctgcgtcccg aattgcagca tttgaatgtt
     4261 gaagacatta cgccgctgca ccgcttggac aaggatacgg caggcgtgat gctgctgtcg
     4321 cataatcctg ccacgcgcgg agcctatcag acgatgtttc aaaacaaaac ggtatggaaa
     4381 acgtatgagg cgcttgcgcc gacaaggacg gatttgccgt atccgctcga tgtggtttcg
     4441 cgtttggtga ggggtgagaa attttttacg acgcaagagg cggaaggcga accaaacgca
     4501 catacgacgg tcgaactgat tgaaaacagg ggggaattca gcctttaccg ccttacgccg
     4561 catacgggca agaaacacca gttgcgcgtg catatgatgg gtttgggtat gccgctgttg
     4621 aatgacgcgc tctatcccgt gccgtctgaa gcgggcagcg aggattatcg gaaacctttg
     4681 aagctattgg caaaaaagat tgcgtttgca gatcctttgt cgggtcggga aagggtgttt
     4741 tgcagcggat tttgcctata atgagaggga acgcaaaccc gtatcggcgg atgccgccga
     4801 tacgggtttt acattatcga tgccgtctga aaggggtttt atccgttcag gcatacgggc
     4861 gcgaagcgga ggatggtgtc gcgcaggacg atgagtttgc cgtgaaagaa atgcgtggaa
     4921 ccggcgatgg taatgacggg caaatcttgc ggttccgccc atttcagtgc tttcccgatt
     4981 tcgacgactt cgtcttccgc gccgtgtatc atcagcgttt tggcaacatt gggaacggcg
     5041 gacggctcgg ggcggccggt atagtggcag actgccgcgc cgatgagcag caataaatcg
     5101 ggtatgcgcg cttgtgcggc aaatgtggcg acataaccgc cgaaggagaa gccggataag
     5161 gcaaattcgg gggcttcggg atgttgggca cgagcatagt cgatgacggc gatacagtct
     5221 tgcgtttcac cgtgtccgta atcgtgtgtg ccttcgctgc cgcctacgcc gcgaaggttg
     5281 ggcaggtagc agtggaagcc gagtttgctt aaggctttgg cggcggtttg gatgactttg
     5341 ttggtgttcg tcccgccttg gagggggttg gggtggttga tgacggcaac accgcgtgcc
     5401 ggtacttgtt cggacgggat atggatggtt tctaaaatgc cggcagggcc gggtatgtgt
     5461 atggtttcag gtttttgcat aatggtttcc tcttgatttt ccgcatattc cgatgcagcc
     5521 gcgaagatgg cggcttaaag gtggaaatgc ttgccgttgt tggggttgga tgctttcagg
     5581 tcggcgagca tgcggtggaa gtccaatccg taagcgttgc tcagtttgtc ggcacgtttt
     5641 ttgagtttgt ctatgttttg ctctttgggg ctgctttgga aggccattac cgcgacattg
     5701 ccgtggcttt cggcaggaag ttccaagacg cgcccttcaa aaacgctcaa caaccgttcg
     5761 atgaagcgtt ggtagcgttt gtcgccgctc caccagttgg ttacgaatat gccgtctgaa
     5821 gagagtgcgt ttcggcagtc tcggaaaaac ggttcttcaa ccagcgcatc gatgatttgt
     5881 tcgccgtcga acccgtccac caaaatcaca tcggtgttgt ggcggaagac tttgatatat
     5941 tctgcaccgt ctgcttcgat aatttcaaat ttctcgccct cgaaaggcaa ctcgaacagg
     6001 ctgcgggcaa tggcgatgac ctgcgggttg atgtccacgg cggtttggcg cgtgtcgggc
     6061 aggtaggtgt ctatccagcg tgcaaacgaa ccgccgccca agccgatttg ggtgatatgc
     6121 tgcggaagag cgtcggtaaa cagcagccat cccatcatgg cacggctgta agagagtacc
     6181 agctcggacg ggtggtcgag gttcattgag ctttggatgg tgtcgctgcc caagtgaagc
     6241 gaacggatat tgccttcttc ggaaatgccg acttcgggaa agctggattt tgcaggacgc
     6301 aggcggcggt agggatgtct tgccatcggt gtgaacggtc ggtttgaaag gcagatattt
     6361 tatcagaatg ccgatggttg tcctgcttca aattaatttc ttttcaaata aattacttat
     6421 tcggatttgc cggggctttc ggataaattc cttgccaagg tgcggcattg cctgcataat
     6481 tcgctttctt tgccggtata gctcagttgg tagagcacct gacttgtaat cagggggtcc
     6541 cgagttcgac tcttggtgcc ggcaccattt ctgctgatgg tgttgcaaaa catttcagta
     6601 agccggtata gctcagttgg tagagcacct gacttgtaat cagggggtcc cgagttcgac
     6661 tcttggtgcc ggcaccagat ttgacagtcc gattgtgtaa aacagtcggg ctgttttgca
     6721 tttgcggcac ggtcggtggc agggctgcgg gcatttcact ataatagccc gtttcaaact
     6781 gaaaaggccg tctgaaaagg gcggggtaac aatatctgat gattactgtg aacacactgc
     6841 aaaaaatgaa ggcggcgggc gaaaaaatcg ctatgctgac cgcttacgaa tccagttttg
     6901 ccgcgctgat ggacgatgcc ggcgtggaaa tgctgctggt cggcgattct ttggggatgg
     6961 cggttcaggg gcggaaatcg acgctgcccg tcagcctgcg cgatatgtgt tatcacaccg
     7021 aatgtgtagc acgcggtgca aaaaatgcga tgattgtcag cgatttgccg tttggtgcat
     7081 atcagcagag taaggagcag gcgtttgccg ccgctgccga actgatggct gccggcgcgc
     7141 atatggttaa actcgaaggc ggcgtgtgga tggcggaaac gactgaattt ttgcaaatgc
     7201 gcggtattcc ggtttgtgcg cacatcggtc tgaccccgca atccgtgttt gctttcggcg
     7261 gatataaagt tcaggggcgc ggcggcaagg cgcaggcgtt gcttaacgat gccaaggcgc
     7321 atgacgatgc gggggcggcg gtcgtgctga tggagtgcgt actggcggaa ctggcaaaaa
     7381 aggtaactga aactgtttcc tgtccgacta tcggtatcgg tgcgggtgtg gattgcgacg
     7441 ggcaggtttt ggtgatgcac gatatgctcg gcattttccc gggtaagacg gcgaaattcg
     7501 tcaaaaactt tatgcagggg catgatagtg ttcaagcggc ggttcgggcg tatgttgccg
     7561 aagtcaaagc caaaaccttc cctgctgcgg aacatatttt tgcagattga acggtttaca
     7621 tgcagcatgc cgtctgaaag ccgtttcaga cggcattttg ttttgccgtg cgcggcgcgt
     7681 ataatcggcg cgttttgtcg ggcaggaagc ccgaaagata aggattaccg ttatgcaaat
     7741 catacatacc attcgagaac tgcgcgcgtg gcgtaaaaat gcgggaaagg tggcatttgt
     7801 gccgaccatg ggcaatctgc atgaagggca tcttgcgctt gtgcgtgagg cgaaaaaacg
     7861 cgcggacagt gtcgtggtca gcattttcgt caaccgcctg caattcggtc agggcgagga
     7921 tttcgacaaa tatccgcgta ctttgcaaca ggatgcggac aagctggaag ccgagggtgt
     7981 tgccgttgtt ttcgcgcccg atgagaaaga actctatccg aacgtggaac agcgttacaa
     8041 cgtcgaaccg cccaatctgc aaaatgagtt gtgcggcaag tttcgtccgg gacatttccg
     8101 cggggtggcg acggtggtgt ccaagctgtt caatattgtc gcgccggatg ttgcctgttt
     8161 cggcaaaaaa gactatcagc agcttgccgt gattaaaggt ttgaccgagg atttgaattt
     8221 tgatgttgaa atagtgcctg tcgatacggg gcgcgcggaa gacggtttgg cactctccag
     8281 ccgcaaccag tatttgagtg cggcggaacg cgacgaagca ccgcgcctgt actgcgaatt
     8341 aaaggctgtt gccgaatcct tggcgcaggg cagtttggat tatgcaggtt tggagcggca
     8401 ggctgccgac catttgaccg ctgcgggctg ggtggtcgat tatgtcgaaa tccgccgcgc
     8461 cgatacgctc gaagtggcgc gggcgggaga taagaaactg gtggtcttgg ccgccgcccg
     8521 tctggggacg acgcgcctga ttgacaattt ggaaataaaa ctcccttaaa ccgcaagcgt
     8581 cgggaatgcc gtctgaagcg gatttgcgtt tcagacagca tttatttttt tgaacggggt
     8641 ttcgcaaatc tacaaacgat tctgcttgtg ataaagttac gcctgattat atgccgtctg
     8701 aaagttcgga cggatgtcgg ataaaggatg attatgctac ctaaccgttt caaaatgtta
     8761 actgtgttga cggcagcctt gattgccgga caggtatctg ccgccggaag cggtgcgggg
     8821 gatatgaaac agccgaagga agtcggaaag gttttaagga aacatcagcg ttacagcgag
     8881 gaagaaatca aaaacgaacg cgcacggctt gcggcagtgg gcgaacgggt taatcagata
     8941 tttacgttgc tgggagggga aaccgccttg caaaaggggc aggcgggaac ggctctggca
     9001 acctatatgc tgatgttgga acgcacaaaa tcccccgaag tcgccgaacg cgccttggaa
     9061 atggccgtgt cgctgaacgc gtttgaacag gctgagatga tttatcagaa atggcggcag
     9121 attgagccta taccgggtaa ggcgcaaaaa cgggcggggt ggctgcggaa cgtgctgagg
     9181 gaaagaggaa atcagcatct ggacggactg gaagaagtgc tggctcaggc ggacgaagga
     9241 cagaaccgca gggtgttttt attgttggca caagccgccg tgcaacagga cgggttggcg
     9301 caaaaagcat cgaaagcggt tcgccgcgcg gcgttgaaat atgaacatct gcccgaagcg
     9361 gcggttgccg atgtggtgtt cagcgtacag gggcgtgaaa aggaaaaggc aatcggagct
     9421 ttgcagcgtt tggcgaagct cgatacggaa atattgcccc ccactttaat gacgttgcgt
     9481 ctgactgcac gcaaatatcc cgaaatactc gacggctttt ttgagcagac agacacccaa
     9541 aacctttcgg ccgtctggca ggaaatggaa attatgaatc tggtttccct gcacaggctg
     9601 gatgatgcct atgcgcgttt gaacgtgctg ttggaacgca atccgaatgc agacctgtat
     9661 attcaggcag cgatattggc ggcaaaccga aaagaaggtg cttccgttat cgacggctac
     9721 gccgaaaagg catacggcag ggggacggag gaacagcgga gcagggcggc gctaacggcg
     9781 gcgatgatat atgccgaccg cagggattac gccaaagtca ggcagtggtt gaaaaaagtg
     9841 tccgcgccgg aatacctgtt cgacaaaggt gtgctggcgg ctgcggcggc tgtcgagttg
     9901 gacggcggca gggcggcttt gcggcagatc ggcagggtgc ggaaacttcc cgaacagcag
     9961 gggcggtatt ttacggcaga caatttgtcc aaaatacaga tgctcgccct gtcgaagctg
    10021 cccgacaaac gggaggcttt gagggggttg gacaagatta tcgaaaaacc gcctgccggc
    10081 agtaatacag agttacaggc agaggcattg gtacagcggt cagttgttta cgatcggctt
    10141 ggcaagcgga aaaaaatgat ttcagatctt gaaagggcgt tcaggtttgc acccgataac
    10201 gctcagatta tgaataatct gggctacagc ctgctttccg attccaaacg tttggacgaa
    10261 ggcttcgccc tgcttcagac ggcataccaa atcaacccgg acgataccgc tgtcaacgac
    10321 agcataggct gggcgtatta cctgaaaggc gacgcggaaa gcgcgctgcc gtatctgcgg
    10381 tattcgtttg aaaacgaccc cgagcccgaa gttgccgccc atttgggcga agtgttgtgg
    10441 gcattgggcg aacgcgatca ggcggttgac gtatggacgc aggcggcaca ccttacggga
    10501 gacaagaaaa tatggcggga aacgctcaaa cgtcacggca tcgcattgcc ccaaccttcc
    10561 cgaaaacctc ggaaataatg caggtccatc ctttcagacg gcataaggtt tgccgggaag
    10621 ccggggcatt cgggcaaacg gcacgcagtt cgcacgcgtt attgcacggc acaccgaacc
    10681 catcggccgg caggatggca tccgttaagg aaattctgat gaaacacact gtatccgcat
    10741 cggtcatcct gcttttgacc gcttgcgcgc aattacctca aaataacgaa aacctgtggc
    10801 agccgtccga acacacccgc agtttcacgg cagaagggcg gttggcagtg aaagcggaag
    10861 ggaaaggttc gtatgcaaat ttcgattgga cataccaacc gcccgtggaa accatcaata
    10921 tcaatacccc tttgggcagt acgctcgggc agttgtgtca agacagggac ggcgcattgg
    10981 cagtggacgg caaaggaaat gtctatcagg cggaaagtgc ggaagaatta agcaggcagc
    11041 tggtcggttt caaactgcca atccaatatc tgcatatctg ggcagatggc aggcgtgtgg
    11101 cgggcgcgcc ttaccgcatc ctgccggacg gcatattgga acaatacggt tggactgtcg
    11161 gcagaaccgc cgacagtggg gggcaagtcc gaacgttgca actgaataac ggaaatttga
    11221 acatcaggct ggttttcacc gaaatcggta tgccgtctga aactgaaacc ctggaacgct
    11281 gtgcggcgcg cacgagataa ggcggacaga tgaatattgc ggacggacgg caggcgtttc
    11341 ccgcacctgc aaaactgaat ctcgatttga ggattactgg aaagcgggaa gacggttatc
    11401 acaatatcga aagcatattc tgcctgatag atttgcagga taccgtatat ttgaaactaa
    11461 gggacgacgg caaaatcatc ctgcacaatc cggttgatgg aatgccgcag gaagtagatt
    11521 taagctaccg tgccgcatcg ttgctgcaaa aatatgcgcg caaccccgcc ggcgtggaaa
    11581 tatggctgga caaaaaaatc ccgacagggg cgggtttggg cggcggaagc tcggatgcgg
    11641 caaccgtttt gctggtgttg aaccgttggt ggcagtgttc ccgaacgacg cagcagctga
    11701 ttgacttggg cgcaactttg ggtgcggacg taccattttt tattttcgga aaaaatgctt
    11761 ttgcaagcgg gataggtgaa aaactgatag gaatggatat tccgaaacag tggtatgtca
    11821 tcgtcaaacc gcccgtccac gtttccactg caaaaatttt cacacacgaa ggcttgacac
    11881 gaaattccgc ctcaagcata atgccgactt tccaaaacct gcaaccgttt agaaatgata
    11941 tgcaggcagt tgtatttaaa gaataccctg aagtttggaa agcctattcc gaattgtccc
    12001 gatatggatt tgccttaatg acaggttctg gtgcgtgtgt attcacggcg tgtcaagata
    12061 gggatagcgc atacaatata taccgacaag tttcagattt gtacgaggca tatttggcag
    12121 agggtctttc aaaacatcct tggttgtccg tataaacatt gttggggagt cgtcaagcgg
    12181 ttaagacact ggattttgat tccagcatgc gaaggttcga atccttcctc cccagccaag
    12241 tcaaacgagt tggggagtcg tcaagcggtt aagacactgg attttgattc cagcatgcga
    12301 aggttcgaat ccttcctccc cagccagccc tttttgggga gtcgtcaagc ggttaagaca
    12361 ctggattttg attccagcat gcgaaggttc gaatccttcc tccccagcca gataaaagcg
    12421 tgtaagcctg cttacacgct tttatttcat agaaataaaa atattgaaat gcctttgttt
    12481 gtgtcggatg ttgcaggtat aatgtcgggc ttggtacaag cagagggaag cattgtgttt
    12541 tctgagcgga agttaaacat aaaatcaggt gagaatatgg ctgcgtacga cagtttgatg
    12601 gtatttacag gcaatgccaa tcccgaattg gcacaacgtg ttgtcaggca tttggacatt
    12661 tctttgggca atgcttccgt atccaagttt tcagacggcg aagttgccgt cgaactgttg
    12721 gaaaacgtac gcgggcgcga tgttttcatc cttcagccga cctgtgcgcc gaccaatgac
    12781 aacctgatgg aaatcctgac gatggcggat gcactgaagc gtgcttcggc aggtcgtatt
    12841 accacagcca ttccgtattt cggctatgcg cgccaagacc gccgtccgcg ttccgtccgc
    12901 gttccgattt ctgccaaact ggtggcaaat atgctgtatt cggcagggat cgaccgtgtt
    12961 ttgactgtcg atttgcatgc cgaccagatt caaggtttct tcgatattcc ggtggacaat
    13021 atttatgcca ccccgattct gttgaacgac atcaaacaac agcggattga aaatctgacc
    13081 gtcgtcagcc cggacatcgg cggtgtcgtc cgcgcccgcg ccgtggcaaa atccttgaat
    13141 gccgacttgg caatcatcga taaacgccgc ccgaaagcca atgtggcaga agtcatgaac
    13201 atcatcggcg atattcaagg cagaacctgt ctgattgtgg acgatatgat tgacaccgcg
    13261 aataccttgt gtaaagctgc cgtcgccctg aaagagcggg gggcggaacg tgttctagcc
    13321 tatgccagcc acgctgtatt ctccggagag gcggtcagcc gtatcgcctc atccgaaatc
    13381 gaccaggtgg tcgtaaccga taccattcct ttgtctgaag cggctaaaaa ctgcgaccgt
    13441 atccgtcagg taacgattgc cggtctgttg gccgaaaccg tccgccgcat tagcaatgaa
    13501 gaatccgtct catatctttt caatgaagaa gtgatgacag gcagcatgtt gctgccataa
    13561 gcccgaagcc gtcttaagct ggtcgcggcc gatgacggcg attttaccta acttggagta
    13621 tttaacatga cttatgaaat tcaagcttct gttcgtgaag cacaaggcac tggtgcgagc
    13681 cgccgcctgc gtcgcgaagg ccaaatcccc ggcattctgt acggtgaagg tcaagagcct
    13741 gttgcaatcg ctgtggatca caaaaccgta ttctacgcat tggaaaaaga atctttccat
    13801 actgcgttga ttaagttgtc tctgaacggt gaaaccaaag acgttatcgt ccgcgatttc
    13861 caaatgcacc cgttccgccg cgaagttcaa cacatcgact tccaagctgt gaaagccgat
    13921 caacttgtac gcatccgtgt tcccctgcac atcgttaacg ctgaaaattc ccaagcggtc
    13981 aaactgcaag gcggccgcgt atctctgtta aacacttctg ttgaagtagt tgctttgcct
    14041 gccaacatcc ctgctttctt ggatttggat tgtgccgaag tggttgccgg cgacattctg
    14101 cacttgtcag acatcaaact gcctgaaggt gtagaaagcg tttccctgaa acgtaacgaa
    14161 aatctggctg ttgctaccgt taccggtaag aaacgctaat tgattgaaaa gcaggcggta
    14221 cgtatatgat gcgtaccgcc ttattgtttt tggctgtgtt tcaatatttg gttgatttca
    14281 tccgtttata agggaaaacc gcttatttcc gttcgggtgg aaatttatcc agacgtattg
    14341 cgcgacaacg gatggttggc tcaaaaacgg catttcgtac cggatgaaaa tgaagaagat
    14401 atgtcgttca cattccggta gctgtccggc tcgaaccggc tatcagagat agtcggtcaa
    14461 ttttcaccct gaaaatcact tttcttttaa cttgcaacat attccgataa aatggacact
    14521 ttgtaagtaa tcattgatgg aagacgcaat tatggcaacc gaaccgaacc gaaccgaacc
    14581 gaaattatac acgatttaga agataatttc aaacaaaaca atatattaaa actcaaacaa
    14641 ctctttcccg aagtcttttg tgaagaccaa attgactttg aaaagctcaa actcgtcctc
    14701 ggcgcagaaa accttgccgg tgcgggcgaa cgttatcaac tcgattgggc gggcaaaacc
    14761 gcctcctatc tcaacctgca aagccccact tcccgcaccc tcgtcccctg caaagaagaa
    14821 tccgcagatt tcgacggcac gcaaaacgtg tttatcgaag ccgaaaacct cgaagcactg
    14881 aaaatcctgc aaaaatccta cgccggcagc gtaaagatga tttacatcga cccgccctac
    14941 aataccggca acgattcctt cgtctatccc gacaaattct ccgaaagccg cgaagaatac
    15001 gcccgccgcg tgggcgatac cgacgacgcg ggttacctga agcgcgacgg cgtgttccag
    15061 ggcgcgtggc gcaaaaacgg caaagacagt ggccattacc acagcaactg gctctctatg
    15121 atgctgccgc gcctgcatct ggcgaaaacc ctgctgcgcg aagacggcgt gatttttatc
    15181 agcattgacg acaacgaaca ggcgcagttg aaattgttat gtgatgaagt gtttggggcg
    15241 gagaattttg tcggtcagtt tccttggcgc aaacgcacgg caaaatcgga cgtgccgttc
    15301 ggcgtttccg ccgattatga atatctgctt gcctatgccc gcagtaatgc ctttattgcc
    15361 agtatcgatg cggaagaacg cagatatttt gaaaccgatg atttcccgaa tcgcccgtgg
    15421 cggacgcatg atttgactac tcaaagaaat gccagtgaac gaccaaatag ttttttcact
    15481 atggttaatc ctaaaacagg caaagaatat ccggcgaatc caaatgcaac ttggcgtgtt
    15541 actaaggata catttcaaga atattacaat gcgggaaaaa tcgttttccc cgatgattac
    15601 gatttcctca aaattaaaaa ccccgttatg cgttatttta aagatgacga catgaagaaa
    15661 gacggagagc gcttcggacg taaggcagtc agtacacggc tacctgaaaa tgtaggtatg
    15721 tcgctgaacg gtacaaaaga aattaccgaa ctgtttaaaa gcaagttgtt tgattttccc
    15781 aaaccgtcat ccctaattaa atatttactg caaatcctcc atgtttcaga cggcctcatc
    15841 ctagacttct tctccggcag cggcaccacc gcccatgccg tgatgcaact gaatgccgaa
    15901 gacggcggca accgccgttt catctgtgtc cagctccccg aagaaaccga cgaaaaatct
    15961 gaagcgcgca aggcgggttt cgacacgatt gccgaaattg ccaaagaacg catccgccgc
    16021 gcaggcaggc agatttcaga cggccttcaa gacggatcgg aaacagatac gggctttaaa
    16081 gttttcaaac ttgccgaaag cggtttcaaa caatggcggc agcccgagca gtccgatacg
    16141 gaagccctgc aacgcgaatt gtcgttgaat atcgattctg tgctgtccga aacgtcgtct
    16201 gaaaatctgc tctacgaatt gatgctgcgt atgggcttga agctgacttg caaggtttcg
    16261 tttttagacg acgtatattt cgtggaagac gaggatacgg gcgggctgta cgctttcctg
    16321 ctgaaacgcg tggatcaagg tttgattgat gccgtgctgg cgaagcatcc cgctaaggtg
    16381 gcggcattgg acaggctgtt tgagggcgac gatgcgctta aaagcaatac cgtattgcag
    16441 atgaaggatg cgggcgtgat gttcgagtgt gtgtagtcag ccgaaagcgg gaagtgccgt
    16501 ctgccgtcat tcccgcgcag gcgggaatct agaaactcaa agttgcaaga atttatcgga
    16561 aatgactgaa acccaacgaa cctagattcc cactttcgtg ggaatgacga tatatcagtt
    16621 tctgcgccga caggtctgga ttcccgcctg cgcgggaatg acggcaaaga ggtagatttt
    16681 ccgcgcgaca gcggatagaa ggaaataaaa atggaactga aatttgaaga actgccctat
    16741 cagcttgatg cggtaaatgc cgttgcgaat ctttttgcag ggcagccgaa ccatgcccgg
    16801 acattcgatt tgaccagtca ggggacgggg cggtttgtcg gcaacggctt ggatttggat
    16861 tgggaaacgc taggcctgaa tctgaacacg gtgcaaaagc aaaatggaca gcctgaaacg
    16921 gaaatcggcg cgcacgggct gaatttttct ctagaaatgg aaacgggtac gggcaaaacc
    16981 tatgtctatc tgcgcacgat ttacgaatta aaccgcagtt acggctggaa gaaatttgtt
    17041 attgtcgtac cgagcgtacc gatacgcgaa ggcgtgttga aaagcctgga aattacgaag
    17101 gcgcattttg acggagtgtt cggcaggccc gtgatgcgtt atgcggaata ttcaagcagc
    17161 cggctatcgg acttgcgcac ttttgcggta aacgaccaca tcgagatttt ggtgatcaat
    17221 atccagtcgt ttgaaaaaga tggaaacgtt atcaataagg taaacgaaag cggggaaacg
    17281 ccgattaagt ttattcagga aacgtgtcct atcgttatta tcgacgagcc gcagaatatg
    17341 gaaacggaag gccgtctgaa cgctttggaa tcgctcaatc cgctctttac cctgcgctat
    17401 tcggcaacgc ataaacaacc gtaccacaag gtttacagcc tgaatcccgt gcaggcatac
    17461 aatttaaagc tggtaaagca aatcgaagtt ttgccggtat tggcggaaaa cgatgtgaac
    17521 ggcgcatttg tggagctgct ggaagtaaag caggcggcca aaggccgtcc gaaagccgat
    17581 ttgaacattc attatcagga taaaaaggaa accaagaaga aaaaggtatc cgtcaaatgg
    17641 gaagacgatt tgtttgaaaa atcgggcggt aacgaagcct accgccacgg tttcattatc
    17701 gacggcatgg atttcgataa gcaggaattg gcgttctcaa gcggtaaaac cattacgcgc
    17761 ggcggagacg gcgatgcggt gcgcgatgaa attatgaaaa accaaatccg cgaaacggta
    17821 agggagcatt tggcgaagga gaaacggctg aatccgctgg ggattaaggt gttgtctctg
    17881 ttcttcatcg accgtgtgga gaattaccgt acagaagacg gaacggcagg caagtttgcc
    17941 ctttggtttg aagaaatcta tcgggaactt gcaggcaata ccgatacggc aggcgtacac
    18001 gacggctatt tctcgcaaga caggaaaggc cgtctgaaaa acaccgacgg cacgacgcag
    18061 gcggacaacg atacctaccg cctcattatg cgcgacaagg aaaccctgct ttcctttgac
    18121 agcccgctgc gctttatctt ttcccattcc gcactgaaag aaggctggga caaccccaac
    18181 gttttccaaa tctgcacgct caacgaaacc cgttcgccgc tgaaaaagcg gcaggaaatc
    18241 gggcgcgggc tgcgtatcgc cgtcaatcag gaaggacggc gcgtgcgcga tgaaaacgtg
    18301 aacatactga ccgttgtgcc gaacgaaagc tacgaattgt ttgccgccaa tctgcaaaag
    18361 gaatacgaag acgaatgcgg tattaaattt gccgccgaaa acatcaaaaa cggcaataag
    18421 aagaaaaaac agttattccg caaagatttc ccgctcgacc ccgaatttca ggcaatttgg
    18481 gacaagttga aatacaaaac gcgctatcgc gtgcgcttcg atacggaaga attgatcaaa
    18541 caggcttcgg aagcggtggc aaacctgccg gaaatccaaa aaccgaaaat ccgcatccgc
    18601 aaggcgcaat taaaacaatc acaaacctac ggtatcgaaa cagttgaaat tgccggccgc
    18661 agccgcgaaa tggacgttca atgggagatt ccggacatac tcggcgagat tcaaagaaaa
    18721 acggggctga cgcggcgcac ggtgtacgaa atcatcatac agtcaggccg tctgaaagat
    18781 gtagccggta atccgcaacg cttcatcgac cttaccgccg aagcggtaaa ccgcagcctg
    18841 cacgcgttga tggcggcagg aatcgagtat gagagaaccg gcgaagtgta cgaccaaaag
    18901 ctgttggaag atatggcgcg cgaaggtatc gagttttacg aaaacggttt tacattcaca
    18961 gtaagcaatc cgcaaaaaac catttgcgaa aactacatcc ctttggattc cggcacggaa
    19021 aaagcctttg cccaaaattg cgaggattat gatgcgaaaa atgaaggagg cgtggcgttt
    19081 tacttcaaac tgcccggctg gttcaaaatc ccgaccccgc tgggcaatta caatcccgat
    19141 tgggcggtgg taaaacaggt tcgggataaa gtgtatttcg ttgcggaaac caaaaacacc
    19201 ggcaaaggca ttcagaccgg cgtggatatg gacaagctgc gccaaaatga gaggctgaaa
    19261 atcaaatgcg gcaagcggca ttttgaagcg ctgggcggtt tggcataccg tgttgtggaa
    19321 aaagtggggg atttaaagtc ggaaaaagag gagcaggctt aaaacaaaca ggtgtaatcg
    19381 tagtatggcg atgttggatt ggggcggcgg caaccgaaga tgcggatatg gtaatggctt
    19441 tgattgggta aaccgatcgg ggactgaaga ttgtttcaga cggtctgttg attttcttat
    19501 ttcggaaatg ggaagatgga aggctattgc cctgtcagac gcgtccaaag cctttgccac
    19561 cggcttcttt tttctacatt ttccagtgcg acgatttctt tttcggcaag ggtgtatccg
    19621 ttttgtctga ttttgatttt tcctaaaatc tgcccctttt ttaccggggc gggaatcggc
    19681 tgtatggttt ccaaaatttg ttcggttgtt ttcgcttcct tatgtggcag agtgatgtag
    19741 gcttctttga ggaagcctgc gcggacggtt tttttgctgc ctccggaaat ttggatttgg
    19801 gcaacggttt tgcctttcgg atatattttg ggcgtatcga aggcttgcaa tgcccggttc
    19861 agcagcttgc tgttgtctga tgcgcgtgtt tccgccgatt ccgaacccaa tgtgatgaca
    19921 aggatgtgcc tgccgttgcc ggagtatgac acggcaaggt tgtagccgcc gctttctgtg
    19981 tgtccggctt tcagaccgtt tacattgttg tccctatata aaaggatatt gcggttgttt
    20041 tgctctatat ttttgaattt gaaagatttg atggaaaaca gcgggtaata ttccggaaag
    20101 tcgcgcatca atgcttcaga cagcagggcg aggtctttgg cggtggaaac ctgtccttct
    20161 ctactcaagc ctgtcgggtt tttgaataca gtgttcttca tgcccaagcg tcgggcttct
    20221 ttgttcattt gttgcacaaa attttcaatc gagccgttgc ccagtcggcc gacaagggtt
    20281 agggcggcat cgtttgcgga tagtgcaatc atgcctttta agagtttgtc ggtgctgacc
    20341 gtatcgccgg gacgtacaaa cattctgctt ccttctgaag cccatgcgga ttcgggtatt
    20401 tttaagtttt cttcagatcg gatatttccc gatttcatgt ttttgaaaac cagatatgcg
    20461 gtcatcagtt gggttagtgc cgccggttcg acaggggtat tgatgttttt ggcggataaa
    20521 atctgtttgc tttgaaggtc gataacgatg tgtgccgctg tgagggtttc gggtgtttgg
    20581 aacgtggggg cggcgtgtac cgccggtttg ttgggcgcgg gcgatgcggc cgttgcgtga
    20641 gaaacgccta agatgatgga aagcaggacg ggcaggattt tatgtgctgt catgaaatat
    20701 tctaattgtg tgcgtgtttc agtctgccga ttatacgctt agggtgtctg atcgggcgga
    20761 tttttcttgc tttcgcgccg tcttgggcgt atggttttgg gttttgcgat tttaataaac
    20821 cgattatccc atattgaatt atgaacacgc cccttcctta ttccaattac ctcatccgca
    20881 tcctgacggc atctgtctat gatgtcgcgg tcgaaacgcc tttggaaccg gcacgcagcc
    20941 tttctgtacg tttgaaaaac aacatccttt tgaaacgcga agatttgcag ccggtttttt
    21001 cgttcaaaat acgcggcgcg tacaacaaaa tgtccaagtt gccgaaagat gcgctcgctt
    21061 gcggcgtgat tgcggcaagc gcgggcaatc acgcacaagg cgtggcattg tccgcacagc
    21121 gtttgggttg ccgtgccgtt atcgtcatgc cggagactac gccgaaaatc aaagtggatg
    21181 cggtcaaaag ccgtggcggc gaggtggttt tgcggggcgt ttcatacaac gatgcctacg
    21241 attatgcgat ggagttggcg gaaaaagaag ggttaaccta tatcgcgccg tttgatgatc
    21301 ctgatgtgat tgcgggacag gggacggtgg ggatggaaat tgtcagccag catcccgatc
    21361 caatccgcgc cgtattcgta ccgataggcg gtggcggttt ggcggcgggc gtggcggcat
    21421 ttatcaagca ggtccgcccc gaaatcaaag ttatcggcgt tcagaccaac gattcctgct
    21481 gtatgaagca gtcggtcgaa gcgggtgaaa tcgtccattt gaaagatgtc gggctgtttt
    21541 cagacggcac tgcggtcaaa gtcgtcggaa acgaaacctt ccgcctctgc aaagaacttt
    21601 tggatgaaat cattacagtc gataccgatg cggtttgcgg cgcggtcaag gatattttcg
    21661 atgacacgcg cagcattacc gagccggcgg gcgcgttggc gttggcgggt ctgaaagcct
    21721 atatcgcccg agaaggcgcg gaaaaccaaa ccctgattgc cgttaccagc ggtgcgaata
    21781 tgaattttca ccgtttgcgc cacgtttcgg aacggagcga attgggcgag ggcaacgaag
    21841 gtatttttgc cgttaccatc cctgaagaac gcggcagctt ccttaagttt gtcaatatat
    21901 tgggaaatag gaatattacc gagttcaact accgctacgg ggacgatgaa aaagcgcata
    21961 tctttgtcgg acttcaagcg gcaggcccgc aggatttggc ggttatcggc agccggttgg
    22021 atgaggcggg attgcccaat gtcgacctga ccgatgatga gattgccaaa atccatatcc
    22081 gctatatggt cggagggcgg acggacaaag tagaaaacga gcgtctggtc agttttgagt
    22141 ttccggagcg tccgggggcg ttggcgcgtt ttttgaacca tatgcagggc ggttggaaca
    22201 ttacgctctt ccattaccgc aaccacggtg cggattacgg gcggattttg gtcggtatcg
    22261 atgtgccgcc gcacgatgcc gccgcatttg acggtttctt ggaaagtctg ggatacagct
    22321 atcacgagga aacgcaaaat gccgcgtaca agctgtttct tgcctgacgc ttgaaagcac
    22381 aatgccgtct gaaagccttt cagacggcat tgcgctttca tggttaaatc gaatattcaa
    22441 tcagttcgtt ttgagagaag acataaacct gtttcggaat cagcttcaat tctttgcctt
    22501 cggcgattgg gtaacgcgcg gcatcgctgc cagccagcgt gatatgcacg tcttgtttgc
    22561 cgtgtttcac caaaatatgc gtcaatgccc cgacggcgtg gactttttcg atttcggcac
    22621 aaatcatcgg tgtttcgtgt tcggcggcga tctgccattc gtgcgggcgg atatagccgg
    22681 tggcggtttg ttcctgccat ttgtattgtg cgtccaattt ccacgcgaag ccgttataat
    22741 gccagaagcc tttttcgatg cgtccttcaa aagcgtcggt ttcgccgagg aactcggtaa
    22801 cgaaggcatt ttcgggtttg cggtaaatag cttcggcgct gccggtttgt tcgattttgc
    22861 cgtggttcat cacgacgatt tcgtcggaaa cttcgagggc ttcttcttgg tcgtgcgtaa
    22921 ccagaatgct ggttacaccc aggttgtgat ggatgtcgcg cagccaggtg cgtaattctt
    22981 tgcgtacttt ggcatccaac gcgccgaagg gttcgtccaa aagcaggagt ttcggttcga
    23041 ccgcaagcgc acgggcgagg gcgatgcgct ggcgttgccc gccggagagt tggtgcggat
    23101 aggattttgc caaatgcgag agctgcacga gcttgagcaa ttcttcgact ttggcgcgga
    23161 tttgtccttt ggacgggcgt tcggacttgg gcaatacggt caaaccgaag gcaacgttgt
    23221 caaacacgtt catatggcgg aaaagggcgt agtgttggaa cacgaagccg actttgcgct
    23281 cgcgcacgtg cttggcggtt acgtcttgcc catcaaacag aatcttgccg ccgtcggcgt
    23341 tttccagtcc ggcgataatg cgtaaaagcg tggttttgcc gcagccggac gggccgagca
    23401 gggaaacgag tttgccggtg gggacgttga ggttgatgtt tttcagcgcg tgaaaattgc
    23461 cgaagtgttt gtttaagttt tggatggtga tactcatact gcgttccttt cggcggcggc
    23521 gagttttttg tcttgtaatt tggtgatgat gttctgcacc gccagcgtcg ccagtgccaa
    23581 aagtgccaat acgccggaga gggcgaatgc gccggtgaag ttgtattcgt tgtagaagat
    23641 ttcgaccaaa agcgggacgg tgttggtttc gccgcgtatg tgtcccgata ccacgctgac
    23701 cgcgccgaac tcgcccatcg cgcgggcgtt ggtgaggatg atgccgtaga gtaacgccca
    23761 tttgatgttg ggcagggtaa cgcgccaaaa catctgccag ccgcttgcgc cgagtatcaa
    23821 tgccgcctgt tcttcgctgt cgccctgtgc ctgcatcagc gggatgattt cgcgtgcgac
    23881 aaaggggaag gtaacgaaca gcgtcgccaa aacaataccg gggatggcga agataatctg
    23941 tatgccttgc gcttcgagcc ggccgcccaa tgccgtgtgc gcgccgaaca ataagacgaa
    24001 cattaaaccg gccaccacgg gcgatacgga aaacggcaaa tcgagcaggg tagtcagcaa
    24061 ctgcttgccg cgaaaatcaa aacgggtcag cagccacgcc atcgccacgc ccaagacggc
    24121 attgacgggg acgacaatca gcgcggtaat cagcgtcaat ttgatggcag accacgcttc
    24181 gggatcgttt aaggatttca ggtacaaatc ccaaccgcct tttaaggctt cgtaaaacac
    24241 ggcgacgagc ggcacgacca gcatcagcag cagaaagccc agcgcggcgg caatcagcaa
    24301 cacgcgcagc cggcgcggtt cggtcaggtt gggattggcg gaatagggtt tcatggcggt
    24361 ggtgttctct ggttggtttt aaaatgtttg gaggtcgtct gaaaaactgt gtttttggga
    24421 gcggattcgt tttcagacaa ccttttattg attaagggaa taaaaacgtc tagctaccgt
    24481 cattcccgcg caggcgggaa tccaccacag ggcaacagga aaacagaaaa taaataaggc
    24541 agccgaaatt caccaatgga ttcccgcctg cgcgggaatg acggtaacag gtatttcaga
    24601 cgacctcaac ccttcgcgcc cgaacgcctg cccaacgccc acagcatcac gttcagcgca
    24661 aacagaatga caaacgaaac cagcagcata aacaacgcca ccgccgacgc gccctgtacg
    24721 tcgaactgtt ccagcttgcc cgtaataatc agcggcagga tttcagaaac catcggaata
    24781 ttgcccgcga taaaaatcac cgagccgtat tcccccgttg cccgcgcgaa catcattccc
    24841 gcgccggtca agagtgccgg cgtgatttcc ggcagcagca cgcggcggaa cgtcgtaaag
    24901 cggtttgcgc ccaaagtcgc cgccgcttcc tcatattcgc ccgataattc ctccaacacc
    24961 ggctgcacgg cgcggacgat aaagggcagg ctgacgacga ccagcgcaat ccaaatgccg
    25021 acgggcgtaa acgcgatttt gatgcccaaa ggctcgaaaa aacggcctat ccaaccgttg
    25081 ggcgcataca gggttgccaa cgcgataccc gtaaccgccg tcggcagcgc aaacggcaaa
    25141 tcaaccagcg cgttcgccag acccttgccc ggaaattcat agcgcaccaa tacccacgcc
    25201 accagcgtgc cgaacacgac attggtcagc atcgcataaa acgacatccg caaactcagc
    25261 cacaccgccg ccaacacgtt cggctcggca atcgtgttcc aaaagccgcc ccagccgatt
    25321 tccgccgcct tcgccgccat catcgcaaac ggcaagacca caagcagcga caggcacaat
    25381 acggtcagcc cgaggctgag tttgaagccg ggcagtacgc cgggcgtttt gagcggtaac
    25441 ataaaacaat gctgaaaata aggaaaagga aggactactt taacgatgcc gtctgaaaaa
    25501 cggaaagaat ggaaagtttg gtacaaagac gaatttgtta taaagcggtt ggcagtttct
    25561 caagcgggcg cgatgtttta aaatatagtg gattaaattt aaatcaggat aaggcgacga
    25621 agccgcagac agtacaaata gtacggcaag gcgaggcaac gccgtactgg tttaaattta
    25681 atccactata acgccttgtt ttgacggaaa accatcatat aaaggaacac ttatgcagat
    25741 tttatctttt cagccggaca ttgcggaacg tatgctggaa ggtacggaag gcgagtcggt
    25801 caacgaaaac gcacaattcg tccgtacgga caacggctat tggattgcgt ggcatgaagg
    25861 cgtagcggca ctgcttgcgc ccgatacgcc gccgggcatt ccctgttttt gggtggaagg
    25921 cgcggaaagc cttgaagagt tgtgcgtcat ggtggaacgc ggcgagtttg acgaagtgga
    25981 agagtttgac ggcgatgacg acgaatggct cgaaacggca cagggttgcg ggcaccacgg
    26041 cgaagcttgc gcctgcggac attaaaggca ttgcaggctt gccgcaaggg ggcaaggctt
    26101 tgccgttttt taaataatcg gtggattgca tctgtttgca atgggctgaa cgcttccctt
    26161 tgtattttat tgaataaaaa aacttatctt ggtattataa ttaaggcagc atcaattatt
    26221 ttgggatggc aataaacgca aagcattgat ttgcgccgat tgcagactta ttatagcagg
    26281 ttgcggcgcg gacttaatgg gaattgttta tagagcatga tggtctgaaa catcattaat
    26341 taaaaatacc attaatccga tttatattta tttcaatttc aatggaaaaa catcaatgac
    26401 aatgatttta agcattttaa gcctgttttt tatcatcaga ctgttatttt tagccgtctc
    26461 tattaaacat gaaaaagcct tgattgccaa aggggcgaaa caatacggaa aaaccaattc
    26521 cacgctgctt gcggcagttc atacgcttta ttatttggcg tgttttgttt gggtatggct
    26581 ttctgacact gcttttaatg gcatatcctt gattggtacg ctgacggtga tggcttcgtt
    26641 tgtgatattg tcattgatta ttaagcagtt gggggagatt tggacggtta aaatctatat
    26701 tttaccaaat catcaaatta atcgttcgtg gttgtttaaa acattccgcc accccaatta
    26761 ttttttaaac atcatacccg aactgattgg catcgcctta ttatgtcaag cgtggtatgt
    26821 tttattgatt ggcctgccca tttatttgct ggtcttattt aagcgtatcc gacaagaaga
    26881 acaggcgatg gcaacacttt tttaacccgt ttcatcaatt atagcggatt aacaaaaacc
    26941 agtacggcgt
//
LOCUS       DAXHPG010000007        26452 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXHPG010000007 DAXHPG010000000
VERSION     DAXHPG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.007"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(92..880)
                     /locus_tag="ACFM6F_000215"
     CDS             complement(92..880)
                     /locus_tag="ACFM6F_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6163709.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAVRKGGFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALETDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(882..1625)
                     /gene="bioH"
                     /locus_tag="ACFM6F_000216"
     CDS             complement(882..1625)
                     /gene="bioH"
                     /locus_tag="ACFM6F_000216"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC6163710.1"
                     /translation="MPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTADEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLAR
                     CGTPSALQEALDAAERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRRLKGSRL
                     VVMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            1624..2349
                     /locus_tag="ACFM6F_000217"
     CDS             1624..2349
                     /locus_tag="ACFM6F_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC6163711.1"
                     /translation="MDFLSRWRRIADAPTIRRCVLCHGSSGVSDGICAGCRDDLAAYR
                     TDAANSCPLCFRHIQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIRTLKHLADLGM
                     VRPLANLMVQNPPDRLADECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQIL
                     PRHTVFRHHRPPQSTLKGSERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            2414..2878
                     /gene="trmL"
                     /locus_tag="ACFM6F_000218"
     CDS             2414..2878
                     /gene="trmL"
                     /locus_tag="ACFM6F_000218"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC6163712.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            2889..3011
                     /locus_tag="ACFM6F_000219"
     CDS             2889..3011
                     /locus_tag="ACFM6F_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6163713.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            3008..3727
                     /locus_tag="ACFM6F_000220"
     CDS             3008..3727
                     /locus_tag="ACFM6F_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC6163714.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            3789..4115
                     /locus_tag="ACFM6F_000221"
     CDS             3789..4115
                     /locus_tag="ACFM6F_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6163715.1"
                     /translation="MNIKHLLLTSAATALLSISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            4197..4781
                     /gene="ruvA"
                     /locus_tag="ACFM6F_000222"
     CDS             4197..4781
                     /gene="ruvA"
                     /locus_tag="ACFM6F_000222"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC6163716.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            4781..4918
                     /locus_tag="ACFM6F_000223"
     CDS             4781..4918
                     /locus_tag="ACFM6F_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163717.1"
                     /translation="MPSEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            4944..6800
                     /locus_tag="ACFM6F_000224"
     CDS             4944..6800
                     /locus_tag="ACFM6F_000224"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6163718.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VNDGMATLSKPHTILDKPRALPLNVPQGAIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            6805..7728
                     /gene="rsgA"
                     /locus_tag="ACFM6F_000225"
     CDS             6805..7728
                     /gene="rsgA"
                     /locus_tag="ACFM6F_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC6163719.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            7752..7841
                     /locus_tag="ACFM6F_000226"
                     /pseudo
     CDS             7752..7841
                     /locus_tag="ACFM6F_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            7950..8213
                     /locus_tag="ACFM6F_000227"
     CDS             7950..8213
                     /locus_tag="ACFM6F_000227"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC6163720.1"
                     /translation="MSKWRGFNRKGNTMKKNAPKSFEEALSRLESLTQAMQGEMPLED
                     ALAAYQEGNELVRYCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            8197..9093
                     /locus_tag="ACFM6F_000228"
     CDS             8197..9093
                     /locus_tag="ACFM6F_000228"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC6163721.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAV
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(9202..9501)
                     /locus_tag="ACFM6F_000229"
     CDS             complement(9202..9501)
                     /locus_tag="ACFM6F_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC6163722.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHMIELA
                     AGFALTAILAYILESRAGSVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(9613..11058)
                     /gene="nuoN"
                     /locus_tag="ACFM6F_000230"
     CDS             complement(9613..11058)
                     /gene="nuoN"
                     /locus_tag="ACFM6F_000230"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC6163723.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALMFTLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPAAGGNNAAKF
                     LLSINALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(11068..12564)
                     /locus_tag="ACFM6F_000231"
     CDS             complement(11068..12564)
                     /locus_tag="ACFM6F_000231"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC6163724.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVNGQLNDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFTILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(12653..14689)
                     /gene="nuoL"
                     /locus_tag="ACFM6F_000232"
     CDS             complement(12653..14689)
                     /gene="nuoL"
                     /locus_tag="ACFM6F_000232"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC6163725.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIG
                     YVAIEPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVL
                     SAWLLYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDT
                     AIIDNGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(14738..15079)
                     /locus_tag="ACFM6F_000233"
     CDS             complement(14738..15079)
                     /locus_tag="ACFM6F_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163726.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            complement(15203..15778)
                     /gene="fic"
                     /locus_tag="ACFM6F_000234"
     CDS             complement(15203..15778)
                     /gene="fic"
                     /locus_tag="ACFM6F_000234"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6163727.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKIVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            complement(15812..16117)
                     /gene="nuoK"
                     /locus_tag="ACFM6F_000235"
     CDS             complement(15812..16117)
                     /gene="nuoK"
                     /locus_tag="ACFM6F_000235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC6163728.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            complement(16114..16785)
                     /locus_tag="ACFM6F_000236"
     CDS             complement(16114..16785)
                     /locus_tag="ACFM6F_000236"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC6163729.1"
                     /translation="MTFSAILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVYRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            complement(16797..17276)
                     /gene="nuoI"
                     /locus_tag="ACFM6F_000237"
     CDS             complement(16797..17276)
                     /gene="nuoI"
                     /locus_tag="ACFM6F_000237"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC6163730.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(17357..18433)
                     /gene="nuoH"
                     /locus_tag="ACFM6F_000238"
     CDS             complement(17357..18433)
                     /gene="nuoH"
                     /locus_tag="ACFM6F_000238"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC6163731.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGVWMISPLNLWK"
     gene            complement(18436..20697)
                     /gene="nuoG"
                     /locus_tag="ACFM6F_000239"
     CDS             complement(18436..20697)
                     /gene="nuoG"
                     /locus_tag="ACFM6F_000239"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC6163732.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLFQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQVANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDMVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLQGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQATVH
                     AAVPAARVNPNTLGRLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(20987..21424)
                     /locus_tag="ACFM6F_000240"
     CDS             complement(20987..21424)
                     /locus_tag="ACFM6F_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163733.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINKL"
     gene            complement(21424..22725)
                     /gene="nuoF"
                     /locus_tag="ACFM6F_000241"
     CDS             complement(21424..22725)
                     /gene="nuoF"
                     /locus_tag="ACFM6F_000241"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC6163734.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVSFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            complement(23115..23588)
                     /gene="nuoE"
                     /locus_tag="ACFM6F_000242"
     CDS             complement(23115..23588)
                     /gene="nuoE"
                     /locus_tag="ACFM6F_000242"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC6163735.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGISYGETTPDGKFTLVEGECMGACGDAPVMLLNNHKMCSFMTEEAIEKKLAELE"
     gene            complement(23588..24844)
                     /gene="nuoD"
                     /locus_tag="ACFM6F_000243"
     CDS             complement(23588..24844)
                     /gene="nuoD"
                     /locus_tag="ACFM6F_000243"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC6163736.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(24834..25427)
                     /locus_tag="ACFM6F_000244"
     CDS             complement(24834..25427)
                     /locus_tag="ACFM6F_000244"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC6163737.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(25440..25922)
                     /locus_tag="ACFM6F_000245"
     CDS             complement(25440..25922)
                     /locus_tag="ACFM6F_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC6163738.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(25913..26269)
                     /locus_tag="ACFM6F_000246"
     CDS             complement(25913..26269)
                     /locus_tag="ACFM6F_000246"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC6163739.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
BASE COUNT         6576 a         7433 c         6720 g         5723 t
ORIGIN      
        1 cactatacat cggcatttcc tttagttctg tcggttttca caaacaaatg ccgtctgaaa
       61 cttggaaacg gcttcagacg gcatctgcac atcatctctt cggaaaaaac tgcaccacgt
      121 tctccccttg cggcagcatc agttttttaa cggcgtgtcc gtacactgtt tccagcgtaa
      181 tactcaaacc gccttcccgt tcaaatattg tcccgacaca cgaacgcgcg gcgttttcat
      241 cgttccacga catcgcccgc caaacgccca gcgtgtccat atccgcccaa aacgtttccg
      301 cctttttgta atccaacacc agcttcgccg tatcggtaac ggggtcgtaa aacccgtttt
      361 cagcaagcat atcgcccaag tcgtgcatat cgggaaacat cacgctgcgg ctttcaatgc
      421 cgttttcttt cagacggcat ttcagttcgg taaaagtgtc gcgcccgaag caggtaaaaa
      481 acagcagccc gtccgtctcc agcgcgcgcg cccagttgtg cagcacagga aggatttgtt
      541 ccgccgccaa cagtccgaga ttcgaccaca acatatcggc acacgcttcg ggcagcggcg
      601 cggtcgggga ttggcagtgt tgcaccacgc ccttacccgt aaacctttgc caaaaaccgc
      661 ccttgcgcac ggctgcggca gccgccaaaa aatccgcacg ggaatcgtat tcttcaaata
      721 ccgcctgcgg atatcgtttc gccagcaggc tgcggctgat gtccgcatcc gccccggcaa
      781 gcaggatatg tttgggcgcg ttgcggacga gtgtcagccg ttggtcggca tgttcggcaa
      841 gatggcggtg aacctgccag catttgtcct gcggattcat gtcaaacccc ttcgacaaag
      901 tcgcggtaca gcgcggcaaa cgcttccgca tggctcaaaa acggcgcgtg tgccgccttt
      961 tccatcacaa ccagcctgct gcctttcaaa cggcggtgca gatactcgcc catgcgcggc
     1021 ggcgtaatcg cgtctttgct accgaacacc agcagtaccg gaacatctat cttgtccaac
     1081 aaatgccgcg catccgccct ttccgccgcg tccaaagctt cctgtaaggc ggagggcgtg
     1141 ccgcagcgcg ccaaatcggg caggattctg cctatgattc cgtccgcatc aggcgtgtgc
     1201 agaagctgga gttgtagaaa ctgtttgata tgtttggcat aatccgaacg gaacgcgccg
     1261 accattttgc ccaatgcagg cgcggcaagc ccttcgggat agtcttcgtc agccgtcagt
     1321 cgggcgaaac tcgccgtcag gcagagcgaa cggactttgt cgggatggcg cgccgccaga
     1381 tacagcgcga ccaatccgcc gagcgaccag ccgagaatgt cggcgggcgt atcgatttgt
     1441 gcggcaacag cgtcggcggc tgcctcaata tcgaagggtt gcgcgaaggg cgcgtcgccg
     1501 tgtccgggca aatcgacggc ggacaccggc cacgttgcag gcaggcgcgg catcaaatcg
     1561 tcgaacatgt ggcggttcgc cccccaaccg tgtatcaggt aaactttttt gacggcatca
     1621 ggcatggatt ttctctctcg ttggcggcgc atcgcagacg cgcccactat caggcgttgc
     1681 gtattatgcc acggttcgtc cggcgtttca gacggcatct gcgccggctg ccgcgacgat
     1741 ttggcggcat accgcaccga cgcggcaaac agctgccccc tgtgtttcag gcacatccaa
     1801 ggcggcgcgg tgtgcggcgg ctgtcagaaa aaaccgcccg cattcgaccg gatgtgggct
     1861 tcgctgcatt acgaaccgcc cgtcagcaac atgatacgta cgctgaaaca cttggcagat
     1921 ttgggcatgg ttcgcccatt ggcaaacctg atggtacaaa acccgcccga ccggcttgca
     1981 gacgaatgtt tcgatttcgt cctgcccgta ccgctaagca gggagcggct gctgcaacgc
     2041 gggttcaacc aaagtgaaag catcgtcggg ttgttggcac aacgctacgg ctggcagata
     2101 ctgccccgac acaccgtatt ccgacaccac cgcccgccgc aaagcacgct caaaggcagc
     2161 gaacggcggc gaaacatcaa aaacgccttt gaaatccgca caccgatacc ggaaaactgt
     2221 aatattctgt taatcgacga tgtctttacc accggcgcga cgctggacga actggcaaag
     2281 acgctgaaaa aatcgggcgc gaaccgcatc tgctgctgga cgctggcacg cacgccaatg
     2341 aaaaaataac taaatttttt gacaccccat ccgccttgtg gcaaggttac gcgcctataa
     2401 gtgattgata tttatgttta ccatcgtttt ataccagccg gaaatcccgc cgaacacggg
     2461 caacatcatc cgcctgtgcg ccaataccgg cgcggatttg caccttgtca aaccgctcgg
     2521 cttcccattg gattccgcca aaatgaaacg cgccgggctc gactaccacg aattcgccag
     2581 cctgacggtg cacgaaaact tcgacgactg cctcaaggca ctcgcaggca ggcgcatttt
     2641 cgccctgacc accaaaggca cggcgcgccc cgatgaaacc gcgtttcaaa aaggcgacgt
     2701 tttactgttc gggccggaaa cgcgcggact gcctgccgac atcctcgaca gcctgcccgc
     2761 cgcgcaaaaa atccgcctgc ccatgcagcc cggcagtcgg agtatgaacc tttccaatac
     2821 cgttgccgtg attctctttg aagcgtggcg gcaacacggt tacgcaggcg gcgtttgaac
     2881 gcaagttcat gccgtctgaa aacctatccg gacacattcc gaaccgccgt ccgcactgta
     2941 cggcggttcg gaacgtatcc ggcgggcatg atgcccattc gtcttcaact caagaacgga
     3001 acacgttttg actttaaccc gaaaaaccct tttccttctc accgccgcat tcggcacaca
     3061 ctcccttcag acggcatccg ccgacgcagt ggtcaaggca gaaaaactgc acgcctccgc
     3121 caaccgcagc tacaaagtcg ccggaaaacg ctacacgccg aaaaaccaag tcgccgaatt
     3181 cacgcaaacc ggcaacgcct cgtggtacgg cggcaggttt cacgggcgca aaacttccgg
     3241 cggagaacga tacgatatga acgcctttac cgccgcccac aaaaccctgc ccatccccag
     3301 ctatgtgcgc gtaaccaata ccaaaaacgg caaaagcgtc atcgtccgcg tcaacgaccg
     3361 cggccccttc cacggcaacc gcatcatcga cgtatccaaa gccgccgcgc aaaaattggg
     3421 ctttgtcaac caagggacgg cacacgtcaa aatcgaacaa atcgtcccgg gccaatccgc
     3481 accggttgcc gaaaacaaag acatctttat cgacttgaaa tctttcggta cggaacacga
     3541 agcacaagcc tatctgaacc aagccgccca aaacttcgcc gtttcgtcat cgggtacgaa
     3601 cctctcggtt gaaaaacgcc gttacgaata cgtcgtcaaa atgggaccgt ttgcctcgca
     3661 ggaacgcgcc gccgaagccg aagctcaggc acgcggtatg gttcgggcgg tattgaccgc
     3721 cggctgacgg ttatttgata ccttattaat atccacattt tccaacccac gatttacaaa
     3781 ggcaaaatat gaacatcaaa caccttctct tgacctccgc cgcaaccgca ctgctgagca
     3841 tttccgcccc cgcgctcgcc caccacgacg gacacggcga tgacgaccac ggacacgccg
     3901 cacaccaaca caacaaacaa gacaaaatca tcagccgcgc ccaagccgaa aaagcagcgt
     3961 tggcgcgtgt cggcggcaaa atcaccgaca tcgatctcga acacgacaac ggccgtccgc
     4021 actatgatgt cgaaatcgtc aaaaacggac aggaatacaa agtcgttgtc gatgcccgta
     4081 ccggccgcgt gatttcctcc cgccgcgacg actgaatttg atacaatccg tgccgtctga
     4141 agcccgaacc ggtttcagac ggcattttgc acccgacact tcaggattcg gcacacatga
     4201 tcagcagact gaccggcaaa ctggttgaaa aaaaccctcc gcaaatcgtc atcgatgtca
     4261 acggtgtcgg ctatgaggcc gacgtatcga tgcagacctt ctacaacctg ccgcccgtgg
     4321 gtgaaagcgt acaactgttt acccagctta tcattcggga agacgcacat cttttatttg
     4381 gttttgccac tgcggaagaa cgcaagacct tccgccaact gattaaggtc ggcggcatcg
     4441 gcgcgaaaac ggctttgggc attttgtcag cgatgacggc agacgagctg gcgcgggcgg
     4501 ttgccgaaga agatgtcaaa cgcctgtctt ccgcgccggg aatcggcaaa aaaaccgccg
     4561 aacgtatggt cttggaactg cgcggcaagc tggtcgcgca tacggtaacg gacgggctgt
     4621 ttgccgccgc acccgccgcc gacgaaacgg aagacatcgt cagcacgctg cttgcgctgg
     4681 gttacagcga acgcgaagca aaagcggcgg tcaaaggcgt tccggagggg acggacgtgg
     4741 gcgaaggcgt gcgccttgct ttgaaaaacc tgctgaaata atgccgtctg aaggcggcgc
     4801 ggcgtttgcc ctgacgaaac cctgcgtttc cgcctcgcgc tgtcttcccg acggcttttc
     4861 ccgtaaaatg gcgttattgt ccttcctttc agacggcatt gtgtttgatg ccgtctgaaa
     4921 ctgttttacc gaaatcgaaa acaatgttga acaaaatatt ttcctggttc gagtcccgaa
     4981 tcgaccctta tcccgaagcc gccccgaaaa cgccagaaaa aggcttgtgg cggtttgtct
     5041 ggagcagcat ggccggcgtg cggaaatgga tagccgccct ggctgcgctg accgccggca
     5101 tcggcattat ggaagccctg gtttttcaat ttatgggcaa aatcgtggag tggctcggca
     5161 aatacgcgcc cgccgaactg tttgccgaaa aaggttggga actggcggca atggcggcga
     5221 tgatggtatt ttcggttgcg tgggcgtttg ccgcgtccaa cgtgcgcctg caaacccttc
     5281 agggcgtgtt ccccatgcgc ctgcgctgga acttccaccg cctgatgctg aaccaaagcc
     5341 tcggttttta tcaggacgaa tttgccggac gcgtgtccgc caaagtcatg cagaccgcgc
     5401 tggcgttgcg cgacgcggtg atgacggttg ccgatatggt cgtttatgtg tcggtgtatt
     5461 tcattacctc cggcgtgatt ctcgcctcgc tcgactcatg gctgctgctg ccctttatcg
     5521 gctggattgt cggtttcgct tcggtgatgc gcctgctgat tcccaaattg gggcaaaccg
     5581 ccgcatggca ggcggatgcc cgctcgctga tgaccggccg cattaccgat gcctattcca
     5641 atatcgccac cgtcaaactc ttctcccacg gcgcgcgtga agccgcctat gccaagcagt
     5701 cgatggaaga atttatggtt acggtgcgcg cccaaatgcg gctggcgacg ctgctgcatt
     5761 cgtgcagctt catcgtcaac acctccctga ccctctccac cgccgcactg ggcatctggc
     5821 tttggcacaa cggacaggtc ggcgtgggcg cggtcgccac cgccaccgcg atggcgttgc
     5881 gcgtcaacgg tttgtcgcaa tacatcatgt gggaatccgc gcggctgttt gaaaacatcg
     5941 gcaccgtcaa cgacggtatg gcaaccctgt ccaaaccgca caccatcctc gacaagcccc
     6001 gggcactgcc gctgaacgtg ccgcaaggcg caatcaaatt tgaacacatc gatttctgct
     6061 acgaagcggg caaaccgctg ctcaacggct tcaacctcac catccgcccg ggcgaaaaag
     6121 tcggcttgat cggacgcagc ggcgcgggca aatccaccat cgtcaacctg cttttgcgct
     6181 tctacgaacc gcaaagcggc acggtttcga tcgacgggca ggacataagc ggcgttaccc
     6241 aagaatcttt acgcgcccaa atcggtttgg tcacgcaaga tacctcgctg ctgcaccgtt
     6301 ccgtgcgcga caacattatt tacggccgcc ccgacgcgac cgatgccgaa atggtttctg
     6361 ccgccgaacg cgccgaagcc gccggcttca tccccgacct ttccgatgcc aaagggcggc
     6421 gcggctacga cgcacacgtc ggcgaacgcg gcgtgaaact ctccggcggt caacgccagc
     6481 gcatcgccat cgcccgcgtg atgctcaaag acgcaccgat tcttcttttg gacgaagcca
     6541 ccagcgcgct cgattccgaa gtcgaagccg ccatccaaga aagcctcgac aaaatgatgg
     6601 acggcaaaac cgtcatcgcc atcgcccacc gcctctccac catcgccgca atggacaggc
     6661 tcgtcgtcct cgacaaaggc cgcatcatcg aagaaggcac gcacgccgaa ctcctcgaaa
     6721 aacgcgggct ttacgccaaa ctctgggcgc accagagcgg cggcttcctc aacgaacacg
     6781 tcgagtggca gcacgactga accgatgccg tccgaacacc cgttttcaga cggcatttcc
     6841 acacccaacc ccaaagaaac catgaacgac accgcccaaa ttaccgccag ctacggccgc
     6901 cgctacatcg tgcgcacgcc cgacggcaca acctacgaag ccagcacgcg caaaaaacgc
     6961 gtcgatttcg cctgcggcga ccgtgtccgc atcagccccg tcaacgccga acaagttgtg
     7021 attgaagatt ttttaccgcg ccaaagcctg ctctaccgcc aagacgcgtg gaaaaccaaa
     7081 ctcattgccg ccaacgttac ccaactcctc atcgtaaccg ccgccgtccc gagtccgagc
     7141 gtgcggctgc tacagcgcgc cctgcttgcc gccgaagccg ccggcatccg cgccgtcatc
     7201 gtcctgaaca aagccgacct gcccgaaacc gccctttggc gcgaaaaact caaattctac
     7261 gaaacgctgg gttatcccgt catcgaaacc cgcgcactgg aaaacgccgg cagcctgcgc
     7321 cccgccctgc aagggcacag caacatcctg ctcgggcaga gcggtatggg caaatccacc
     7381 ctgaccaacg cccttttggg cagccaaacc gcccgcaccg gcgacatttc cgccgcactc
     7441 gactcgggaa aacacaccac cacccacgcc cggctttatg atttgaacgg cgaaacccaa
     7501 ctcatcgact ccccgggttt gcaagaattt ggtttacacc acctccaagc cgccgatttg
     7561 ccgcgctatt tccccgattt ccgccacctt gtcgggcaat gccgcttcca caactgcacc
     7621 caccgcgccg aacccggctg cgccttcaaa gccgccgcgc aaaccggggc ggcaagcccc
     7681 gaacgcctcg cctttttgca gggcatcacc gacgaactgc ccgggtaacg ccttgccgcc
     7741 tgggcggaaa aatgccgtct gaagccggat tcgggtttca gacggcatcc gtttttcaaa
     7801 aatgctacaa tccgcttttt actggaacat ccgaaactat gttccaacac gcaggacgac
     7861 acttaaagca ccgccctatg tgtcgtcctg atttggaagg gcttacaccc ctcccaaata
     7921 aagtctgatt ctaccgcccc gaaggacaga tgtccaagtg gcggggtttc aaccgaaaag
     7981 gaaacacgat gaagaaaaac gccccgaaat cgtttgaaga agccttgtcg cgccttgaat
     8041 cgctgacaca agctatgcag ggcgaaatgc ccttggaaga cgcgcttgcc gcctatcagg
     8101 aaggcaacga gctggtcaga tactgccaaa ccaagctggc gcaagtcgaa cagaaattac
     8161 aggttttgga cgcggacggg ccgaaggagt tgaaccttga atccgacgaa tgatttgaaa
     8221 gcgtggcaac agagggcgca ggcgcaaaca gagctgctgc tggaacgctt tttgccgtct
     8281 gaaaacgaaa taccgcacac actgcacgaa gccatgcgtt atgcggtttt ggatgggggc
     8341 aaacgtctgc ggccgatgct ggttctggca gcttcggaat taggcgaagc cgtgcatgaa
     8401 gcagtagaac aggcaatggc ggcaatcgaa atgatccatg cctattcgtt ggttcacgac
     8461 gatatgccgg caatggacaa cgacagcctg cgtcggggca aaccgacctg ccacatcaaa
     8521 tatggcgaag ccaccgcgct actggtcggc gacgctttgc aaacccaagc cttcgacgtg
     8581 ttaagccgtc cgacagaact gcccgcagca cgccagttgg caatgttgtc ggtgttggcg
     8641 aaagcgggcg gcagcgcggg catggcgggc ggtcaggcaa tcgatttggc gaatgtcggc
     8701 aaacaaatgg ttcaagccga tttggaacag atgcacagcc tgaaaacggg tgcgctaatc
     8761 cgtgcggcgg ttttattggg ggcgatgtcc tgcccgaatc tgtctgaaga cagtctggcg
     8821 gtattggacg attatgcggc aaaactgggg ctggcgttcc aagtcattga cgatgtgttg
     8881 gattgtgaag cggacacggc gactttgggc aagacggcgg gcaaagatgc agacaacgac
     8941 aagccgactt atgtgaaact gatgggcttg gaagcggcgc gctcatacgc acacaaactg
     9001 gttgccgaag cggtcgcgct gctcgaaccc ttcggcgaca aagccctgcg cctgcggcag
     9061 ttggcagaat ttgcagtcgc ccgcaaatat taaaaccggc gtatatcgcc ggctgaaacg
     9121 tgccggcaca gcgatgccgg cctgcacttg aatgaaacaa atgccgtctg aacaggtttc
     9181 agacggcatt tctatgcttg tctattccct gttgcgcgtg tgccaaaaat accgccacac
     9241 aaaacatgga aacgcaaaaa tcaggtacag gcagacgact gtggcataaa actcccaacc
     9301 ctgatcgtgt accgatcctg cacgggattc gagaatatag gcaagaatgg cggtcagtgc
     9361 gaaacctgcc gccagctcga tcatgtggtg tccgaaatgt ttgcgcttga gcgcggccac
     9421 gccgaacagt ctggtggtga ggaagggggc gttggcaaag atgagtgcca agaccaaaag
     9481 gatgtacatg gatgcggtca tggtgggctt tctgtttggg cattgtcggc ggcgatgccg
     9541 tctgaactgc attgcggaac ggcatatctt aacaaaaacg gcagcctctg acacggctgc
     9601 cgttgcggcg gcttacagcg tgttctccaa cgccttggcg caccagtcga taacggtttg
     9661 cggcataatg ccccacagca ccaacagcag cgcgttgatg cttaagagga attttgcggc
     9721 attgttgccg ccggcggcgg gacgggcgcg gccggattcg tcaaaataca tgactttgac
     9781 cacgcgcagg tagtagaacg cgccaatcag cgacatcacg acggcaaata cggacaacca
     9841 aacatagcct tgttttaaga gtgccataat cacgccgaat ttggcgtaaa agcccatcag
     9901 cggcggaatg cccgccatag agaacataac cagcagcatc aaaaaggcaa gccatacgcg
     9961 gtgttggttc aaccctgcca aatcgctgat gttttcgcac tcgttgtccc cgtccgacaa
    10021 caccatcaac acaccgaacc ctgccgccgc catcagcgcg taggtaatgg cgtaatagag
    10081 acccgccgca aagccgaccg cgcccgccat aaacgccagc aggataaacc ccatatgcga
    10141 tacggtggaa taggcgagca tacgtttgat attggtctgc atgatggcgg caaggttgcc
    10201 gaccagcagc gaggcggcgg caagcagggc aaacatcaga gaccagtcat gatgcacggt
    10261 tcccaacccg gtaacgagga tgcggaaagc gaaaacaacg gcggcgattt tcggggcagt
    10321 gccgaccagg gcggtaacag aagtgggcgc gccgtgatac acgtcgggca cccacatatg
    10381 gaacggcacc gcgccgagtt tgaacgcaac ggcgacgacg ataaacacca gacccagttt
    10441 caacagccat tcgtttgctt cttcattgaa ggaagaggcg agcacgccgg caaattccag
    10501 cgaaccggtt gcgccgtaaa ccatagaaat accgtagagc agcaggccgg atgccagcgc
    10561 acccagaaca aaatatttca aggcggcttc ggcggcaaag ccggaatcgc ggcgcagggc
    10621 aatcagggcg taaagggcaa gcgacaagag ttccaaaccg atataggcag ttaaaaaatg
    10681 ccccgcgctc accatcacgc ccatacccaa gagggcaaac agcgacaggg tgtaaaactc
    10741 gcctttgaaa ataccgcgca cttggttgta gggcttggca tagacaaaca gggtaaacat
    10801 caaggcatat aaaaccattt ttgccaaacg cgacataccg tctgcaatat acatcccgtt
    10861 gaacgaagac gtgctgccct gttcccacac cgccaactgc accacagccg taaccgccac
    10921 cgttgccaac gcgccgtaat gcgtccaccg gcgtttgtca tcactgaccc acaagtccgc
    10981 cagcaacaat aacaccagca gcgcaagcag cacgatttcg ggcatggcgg gcattaaatt
    11041 caaatcagac cagttcattt acacacctca aatcttgctt tgtgccacat gggcaatcaa
    11101 atcgtttgcc gcctgatgca ccacttcgat aaatgcgttc ggatacaggc ccatacccaa
    11161 taccgcaacc gccaaaaccg ccagaatcgt aaactcgcga cagttgatgt ctttcatttc
    11221 ggcaacgtgc ggattgtgaa tcgcgccgaa aatcacgcgc ttgtacatcc acagggtata
    11281 agacgcaccg taaatcagcg tcatagccgc caacgcgccc acccagaaat tcactttaac
    11341 cgcgcccata atcaccataa actcgcccac gaagccagaa gtcgccggca gaccggcatt
    11401 ggccataccg aacaacatca taaacgccgc aaacttaggc atcacattga ccacgccgcc
    11461 ataatcggca atattgcggg tatgcaggcg gtcgtacatc acgccgatac acataaacat
    11521 cgcggcagac acaaaaccgt gggaaatcat ttgaatgatt gcgcctttca atgcccaatc
    11581 attcaattgg ccgttcacaa acaggaacat acccaaagtg acaaaaccca tatggctgat
    11641 ggacgaatac gccaccagtt ttttcatatc ggtttgcacc aaagccacca taccgatgta
    11701 aatcacggca atcaggctca atacaatgat cacaggggca aaatagcgtg ccgcatccgg
    11761 cataatcggc aggataaagc gcaagaaacc atacgcaccc agcttcagcg taatcgccgc
    11821 caacaccatc gaaccgccgg tcggcgcttc aacgtgggca tccggcaacc aagtgtgcac
    11881 agggaacatc ggcactttta cggcaaatga caggaagaac gctgcaaata aaagctgttg
    11941 tacgcccaac ggaatctgtt tgatgttttg gaaatcgaca atagagaagc tgcctgtttg
    12001 atagtacagg taaaccatag caaccagcat caggagcgaa cccatcagcg tatagaggaa
    12061 cagcttgacc gacgcataga cgcggcgcgg gccgccccat acaccgataa tcaggtacag
    12121 cggaatcagc ataccctcga agaacacata aaacagaatc gcatcctgcg cggcaaacgc
    12181 accgttaatc aaacccgaca tcatcaggaa tgccgccata tactgcgccg ggcgtttctg
    12241 aataacttcc caaccggcca ataccaccag cagcgtaata aacgcattca agatgataaa
    12301 gagcactgaa ataccgtcca cgcccaatgc gtagttgatt ttcagaagcg gaatccactc
    12361 gtggaactcg gtaaattgat agccgccgct caaacggtcg aaaccggtaa acaggggcag
    12421 cgttaccaag aaaccggcaa gcgcacccat aaaggcaagc acacgcgcca gcggcgcacg
    12481 gctgtcctta ccggtcgaca aaaccagcac acctgcggcg atgggtatcc atattgccaa
    12541 gctgagtagg tagttggaaa acatagtggt taacctgtgg ttaaaataaa aatatcgttg
    12601 tacgggttgg tttgtcagac gacgttttaa gagaccgtct gaaacctcat ccttatctga
    12661 acaatcccca gaaggtcata ccgagcaaga ccaatacgcc gaacaccata gcggcggcgt
    12721 aggtatagat aaagccggtt tgagctttac gcacttgcgc ggcaatcgcg ccgaccagtt
    12781 tggcagagcc gttgacgatg ccgttgtcga taatggcggt atcgccgact ttccagaaga
    12841 aagtacccaa tgcgcgtgtg cctttggcga aaacgttgaa atacagggcg tcgaggtagt
    12901 atttgttttc aaacaaaacg taaatcggac ggaacgtctg tgcaattttc gctggcaggt
    12961 gcggcagttt gacgtacaaa agccatgcgc tcaacacgcc tgcgatagca aggtagagta
    13021 caggcgaatg caggctgtgc gacaccattg ccaatgcgcc gtggaactct tccttcatga
    13081 tgtgcatggt cggatgcgcg tcggcgttga cgaaaatcac atctttgaag aaatcgccgt
    13141 aaagcatagg ctcgatggca acgtagccga tgatgacgga cggaatggca agcaaaatca
    13201 aaggcagggt aacaaccaac gggctttcgt gcggattgtc gtttttgccc aagctgtgat
    13261 gttcttcgcc atgatgatcg ccatgatgat cgtcatgatg ttcaggcaag ctgcgccatt
    13321 tctcttcgcc gtggaacacc ataaagtatt ggcggaacgc gtagaacgcg gtaacgaaca
    13381 cgctggcaag gacggcaaaa taggcaaagc cgctgcccgg cagggtgctg tatttcgccg
    13441 cttcgataat cgaatcttta gaatagaagc cggagaagaa cggcgtaccg atcagcgaca
    13501 agttaccgat cagcatggtc agccaagtaa tcggcatgta ttttttcagg ttgcccatgt
    13561 ggcgcatgtc ttggtcgtgg tgcataccga taatcgcgct gcctgccgcc aagaacaaca
    13621 aggctttaaa gaaagcgtgg gtcatcacat ggaacatcgc cacggaatag gcagacgcac
    13681 ccaaagccac ggtcatatag cccagttgcg acaaggtgga atacgcaacc acacgtttga
    13741 tgtcgttttg aatcacgccc aagaaaccca taaacagggc ggtaatcgca ccgatcacca
    13801 taatgacaga cagcgcagtg ctgctcatct cataaatcgg cgacatacgc gataccataa
    13861 acagaccggc ggtaaccatc gtcgcagcgt gaatcaatgc agaaatcggg gtcgggcctt
    13921 ccatcgaatc aggcagccaa acgtgcagtg ggaattgtgc cgatttaccc atcgcaccga
    13981 caaacaggag caaacaggtg acagtaatca acgaccattc cacaccgggg aaaagctgga
    14041 tggtggcatt ttgcacgttg ggcagataag cgaatacatc ttgatagcgc aggctgccgc
    14101 cgaaataggc aagcaccaag ccgataccga gcaaaaagcc gaagtcgccg acacggttga
    14161 tcaaaaaggc tttcaggttg gcaaatgttg cgcttggacg tttgaaatag aaaccgatca
    14221 agagatacga caccaagccc acagcctccc agccgaagaa gagctggatg aagttgttgc
    14281 tcataatcag catcaacatg ctgaaagtaa acaaagaaat atagctgaag aagcgttggt
    14341 agccgacttt ttcatcgtgc atatagccga tggtgtagat atgcaccatc aacgatacgc
    14401 ccgttaccac gaccatcatc atcgccgtca tcgtatcgac caagaagccg acggagaaat
    14461 ccaaaccgcc catcgtcagc caggtataga cgttctcatc aaacttggca cggctgccat
    14521 tgaggaatcc ccacagcaca taagccgaca gcacggcaga caccgccaca ccgagtatcg
    14581 taacagtatg cgcaccggca cgaccgattt tgttgccgaa caaacccgca atcaacgagc
    14641 ctgccaacgg aacaagggca attatcaaat ataaagtcat gtcgttcatt tgattgaatc
    14701 cgattgattt aaaaatctat gtttgtttcg tacaaaatta cttcggaaaa acaaatccaa
    14761 cacgctccaa tcgtttgcgt gccacagcta attgctcttc agtaaataaa tcacaccacg
    14821 gcttttgtaa caccagatat tccatactgt atttcaaggc gtggactccg ccaccactca
    14881 gaatcagctc tgtaaaaccg gtctgagtct tcttttcccc cgtacttaat aatttatccg
    14941 ccgcctcttt accaccaaat tcatttacaa tttgtaaaaa tcgtgtcgcc ttgtaaggtt
    15001 gcggcaaatt caaagcctcc tgataaatat ttaacatggc tttatgaaat tcttgttcta
    15061 actgattttt atccatcatt cttcttccaa tatttcagac cggattattc ttacccagaa
    15121 tttcttttct catccgctcc cgtctgatca cctaccgaat caggtcgtct gaaacagtct
    15181 gaaatcgctt ttcagacgac cctcagcctt tttcataccc ttcgtaataa tacgactgct
    15241 cgataccttt aaagatgatt tcacggttgt ccacatcgtc ggtcaggttg tcctttaaca
    15301 gaaagcgcag ttctaaatcg ttgacggggc tgcgttccat cgcctgcaaa tacagggttt
    15361 tgcttacatt ttgccagttt acgatttttt tcaggttttt cttcagcacc aaatccagcc
    15421 agatgcgggt acttctgcca ttaccctcca aaaacggatg ggcaatgttc atttcaacat
    15481 atttggcgat gatttcttca aaagtccgct cgggcatctg ctcgatttta accaaagcct
    15541 cttttaaata catggcgttg gcaaaacgaa aaccgccttt ggaaatgttg tcttccctga
    15601 tttgacccgc aaaatcatat aagccgccga acaggtaacg gtgaatctgt tgcaggcccg
    15661 cggtggtacc gacttcgata cggtcgatgt cgccgctttc aaacaggcgg cgggcattat
    15721 gcaggctttg ttcgtctatg gatttcatcg tttttcctat cgggttttca gacggcatcg
    15781 gtgtcttgtc aggtctcgac ccaacctacc tttacccttt caactcgtcc aaatcggcaa
    15841 cattaatcgt ttgtcggttg cggtacacca ataccataat cgccaaaccg atggcagatt
    15901 cggcagcggc aacggtcaat acgaagaata cgaaaatttg tccggcagta tcgcccaaat
    15961 gttgcgagaa ggcgataaag ttgaagttca ccgccaagag catcagctcg atggacatca
    16021 gcaataccag cacgtttttg cggttcataa agatacccat tgcgctgata ccgaacagga
    16081 gcgcacccaa taccagataa tgcgtcaagg taatcatgct ttgccctccc cttccggctt
    16141 gaggccgtct gaaacttcgc tctcttcggc agattcgact tgcggtttga ccgcttccat
    16201 tttcaccaga cgcatacggc cttgatcggc gcgtacttta acttggtcgg caggatccat
    16261 gcgtttcgga ttggtggttt tacggtaaac cagcgcaatc gccgccacca tacccaacag
    16321 cagcaatacc gccgccaatt caaacggcaa caggtagtcg gtataaatac ggctgcccaa
    16381 atcgcggata ttgttgtaat cggccggaat gtctttcatc agaccaaatg cggccaggtc
    16441 ggttttcggg ttgaccagaa tcaggatcag ggcaaccgcc aacaatgtgc cgaccacacc
    16501 ggcaaccggc gcatgacgcc agaaaccggc gcgcatttct tcaatgtcga tgttcaacat
    16561 catcacaacg aacaggaaca acaccatcac ggcgccgacg taaaccacca ccagcgtcac
    16621 gcccaaaaac tcggcctgca tcagcatcca aagcatcgcg ctcacgcaga aggtcagcac
    16681 cagatgcaaa gcggcgtgga cagggttttt ggcggtaacg gtacgaaccg caccgtacaa
    16741 aacgatggcg gcaagaatat agaacagaat cgcggaaaaa gtcattcgtc tgctccttaa
    16801 cgatacggcg cgtcagcggc tttgcgtttg gcgatttcag cttcgtattt gtcgccaatg
    16861 gccaaaagaa tcggcttggt catgtgcaag tcgccttttt tctcgccgtg gtattcaaaa
    16921 atatgggttt ccacaatcgc atcggtcggg caggcttctt cgcagaaacc gcagaagatg
    16981 cacttggtca ggtcgatgtc gtaacgcttg gtgcggcgcg tgccgtcttc gcgttcttcc
    17041 gattcgatgt tgatcgccat tgcggggcaa acggcttcac acaatttaca cgcgatgcag
    17101 cgttcctcgc cgttcggata acggcgttgc gcgtgcagac cgcggaaacg cacggattgc
    17161 ggcgttttct cttcgggaaa ataaattgtg tctttgcggg caaaaaagtt tttgagcgtt
    17221 acgcccatgc cttttaccaa ctcgccgagc agaaaggttt ttactaagtt agccatatta
    17281 tgttccctca aaacagggat ttcgttaggt attcaaaatc gctttgttca gacggcctca
    17341 agatgccgtc tgaaacttat ttccacaaat tcagcggtga aatcatccac acgcccaaaa
    17401 tcacgatgta ggcgaagccg atcggaatca gcactttcca gcccaaacgc atgatttggt
    17461 cgtaacggta gcgcgggaag gttgcacgga tccacagata ccagtacaac actgccgcca
    17521 ttttcacaaa catccagaat gcggaaggcg taccgataaa gccccagctt tgcgggaacg
    17581 gagacaacca gccgccgaga aacatcagcg atgtcagcgc ggcaatcaga atcatgaaaa
    17641 tgtattcggc aaggaagaac agcgcgaatg cgaagccgga atattcaacg tggtgtccgg
    17701 caacgatttc ggactcgccc tctgccacgt caaacggtgc gcggttggtt tcggcaacgg
    17761 cggaaatcag ataaacgatg aagatgggga agagcggcag ccagttccac gagaacacgg
    17821 aaccgcccgc gatgcctttg gcttgtgcgg caacgatgtc ggagaagttc atgctgcccg
    17881 ataccatcac aacgcacacc agcgcggcac tcatggcaat ctcgtaggaa atgctttgtg
    17941 cggaagcacg cattgcaccc aagaacgaat atttggagtt ggaagcccag cccgcgatga
    18001 tcacgccgta aaccgacagc gaggtaatca tcaggatgta caaaaggccg atattgatgt
    18061 tggtcagcac ccattcttcg ttgaacggaa tcactgccca tgctgcgaaa gacggagcaa
    18121 gcgacataat cgggccgatg tagaacaggg ctttgtttga cagcttcgga cgggttacct
    18181 ctttaaacaa gagtttgaac acgtcggcaa acggctgaat cagaccccac gggccggtta
    18241 cgttcggacc gacgcgaagc tgcatgaagc cgatgacttt acgttcgaaa tacgtcaggt
    18301 aggcaacggt cagaatcagc ggaatcagga taatcacgat tttgacgatg acggatacca
    18361 ccaagcctac ggtgatgccc aaatcgccca gaccgagcgt tgcggcaaag aggttttgga
    18421 accattcttg catgatcaag ctcccgccag ttcaatagtg tccatcaacg cacccagcgc
    18481 ggcattttcg gtatgcagcg gcagatgcac cacgttttca ggcaaaccgg catcggcttt
    18541 gacagcaacc gatacgcttg cgccgttttg tttggcgaca gcggtttgtc cgtcttgcag
    18601 gcccaagcgt cccaaagtgt tcggattcac acgcgcggca ggcacggcgg catggacggt
    18661 tgcctgcaac ggtgcggaac ggcgcacgat agaatcagtg tgataaatac cgacgccgcc
    18721 gacacggacg aggcggtctg atgccgtctg aacgccctcc cctgtccatg tgctgcggtt
    18781 gtccagtttg gacggcaggc tttccgcatc caacgcatct ttcagaatcg cagcggtatc
    18841 gtggtattca aaaccttgca ggtcaaacag gttgcccaat acacgcaaca ctttccacat
    18901 cggacgcgaa tcgccgaagc cttgtaccac gccgtgaaag gattgcagac ggccttccat
    18961 attgatgaag ctgcccgatg tttcggtaaa cggcgcaatc ggcagcaata cgtcgcacac
    19021 gtccagcagc gtttcgctga caaacggtgt aaacgccatc acgcttttcg cctgtttcaa
    19081 cgcggccacg gcttttgcac cgtcaaccat atcgatttcc ggctcgacgt tgagcagcaa
    19141 gactgcctgt ttcggcgcgt ttgccatttc ggcaacgctc tcgccggagt ttacgcccaa
    19201 gacatccgca ccgacgctgt tggcgacttg cggcaaaata cccagcactg cgccggtcgc
    19261 gtctgccagc tcttgcgcgg cggcataaat ggcggcgtaa tcaggatggt tttgcacttc
    19321 cgcgcccaaa atcaccgctg ctttttcagc gtttttcagg ctggcggtaa cggcgtgttc
    19381 cgcattggca gacaggtttt tcagacggcc tgcccactcg tcgggatgtg cggcttcttg
    19441 aaacagaagc ggcataaaca attcttcctt gctgccggcc aatacgctca aggccatacg
    19501 gtctttggcg gcgcggcgca ggcgggcggt caggagcggc tgttctttgc gcaagttcgc
    19561 gccgactacc aatacggcat cgttgtcagc caaagattca atgctttgtc ccaaccattg
    19621 cgcaccctta atgccgtctg aaagacgttt gtcttgttga cgcaaacggg ttgcaaagtt
    19681 tttaacaccc aagccgtcgg caagtttttt ggccagatac agctcttcaa ccgtattcat
    19741 cgggttcgcc caaatgccga cttggttttg gttgccgtct ttggcgatac attcaatcgc
    19801 gctgcggaca tattccaatg cggttttcca atccacgtcc atccactcgc cgccctgttt
    19861 gattttcggg tttttcagac ggctttcgtg atacaggcct tcgtaggcaa aacggtcgcg
    19921 gtcggacaac cagcattcgt taatcgcttc gttttccagc ggcagtacgc ggcggacggt
    19981 atggtctttg gtttgtacaa tcaggttact gcccaaagcg tcatgggcgg aaacggattt
    20041 gcggcggttc aattcccaag tacgcgcgtt gaagcggaac ggtttgctgg tcaatgcgcc
    20101 gacaggacac aaatcgatga cgttgcccga cagctcggtt tccaccgttt tgccgataaa
    20161 gggcatgatt tcggagtgtt cgccgcgatt caccatcgca atttcttgca aaccggcgat
    20221 ttcttcagtg aaacgaacgc agcgggtgca gtggatacag cggctcattt cctcggcgga
    20281 aaccaaaggc cccatgtctt tgccgacgac ggaacgtttt tcttcggtgt agcggctggt
    20341 ggtttttccg tagcccaccg ccaaatcctg caactggcat tccccgcctt ggtcgcaggt
    20401 cggacaatca agcggatggt tgatgagcag gaactccatc acgccttcct gcgcctctcg
    20461 ggcttttgcc gaatgcgtac gcacaatcat gccgtctgta accggcgtgg cacaggcagg
    20521 caggggtttt ggggcttttt ctacgttcac cagacacata cggcagttgg cggcgatgga
    20581 aagttttttg tggtaacaga aatgcggaat ataagtaccg agcttgtgcg cggcttcaat
    20641 caccgtcgcg ccctgctcca cagatacttg tttgccgtcg atttcgattt gtaacatggt
    20701 tcgttcctag ttacgggtat ttgataaata attttttatg gagatcgtct gaaaaatggg
    20761 tttcagacgg ccttttgagt ttcattgaca atcaatactg tttaaaacgt ttactgccac
    20821 cttgccgtca ttcccgcgta ggcgggaatc cattcttggg ttttcggtaa ctgcttttca
    20881 aatatcgggt tctgtaaatt ccactatgga ttcccgccta cgcgggaatg acggcaaagt
    20941 taaattttta gcattttgct tttaaccaat ataaaaccaa ctaaaactat aacttattta
    21001 ttatattttt aaatgactta aataactctt taacagcacg ctcattgtct tcatctgtag
    21061 gctcaaactt ttcacaccaa ttaattatat ttacatcgcc atgcaataac tctgcataac
    21121 atcctactgc atcgcaacta ccaccccgtg tccttgacgt gccaaaaata aaatcacaat
    21181 tatgcgaata aagtttgtat aaatttctcc taacctcgta ttcattatca ccagatgaat
    21241 aaaaaccaat cctctgacct tcatgatcaa atactgcaac aaaatctaag ccgtttccat
    21301 gtctttcaaa aaagactaga aatttagaaa atttccgaca aatctgatta aaaagcgtat
    21361 tgagtgtcgt actcttacct ttgttggctg caccgtatag aataaaaatt ttagcttcca
    21421 ccattaccac cctccccact tatgctcttt catcggtccg ccgtgttcga tgtaatgcac
    21481 aaactcatca cggaaatgct tggtaaagct tcggacgggg aagacggcgg catcggcaag
    21541 ggcgcagatg gtgcggcctg ccatttggtt gccgacagaa tccagcaaat ccaaatcttc
    21601 cattttacct ttgccttcta cgatgcggtg gacgatgcgg taaagccagc cggtgccttc
    21661 tcggcaaggc gtacattggc cgcaagactc gtcgtagtag aagtagctca ggcgctcaag
    21721 ggctttcacc atgcacacgt cttcgtccat gacgataatc gcgccagaac ccagcatcga
    21781 gcctgctttg gaaatcgagt cgtagtccat atttgtctgc atcatgatgt cggcaggcaa
    21841 tacgggcgcg gacgaaccgc cgggaatgac ggctttgagt tttttgccgc cgcgcatacc
    21901 gcctgccatt ttcaagactt cggcaaacgg tgtgcccaat ggcacttcat agttgcccgg
    21961 acgctcgaca tggccggaaa tacagaataa tttggtaccg cctgcattcg gaataccttt
    22021 atcggcaaat gcctgtccgc cgtcacggat aatgaatgaa acggaggaga aagtttcggt
    22081 attgttgatg gtagtcggtt tgccgtacag gccgaacgaa gcagggaatg gcggtttaaa
    22141 gcgcggctgg ccttttttgc cttccagcga ttcaagcaat gcggtttctt cgccgcagat
    22201 gtatgcaccg tagccgtggt gggcgaagag ttcgaattca aaatccgaac ccaaaatatt
    22261 tttacccaaa aagcctgcgg cacgcgcctg ctccaaagcg gcctcaaagc gttggtagcc
    22321 ttcaaaaatt tcgccgtgga tatagttgta accggctttc gcgcccatcg cgtaaccggc
    22381 gataatcatg ccttcaatca gggcatgggg gttgaacata ataatgtcgc ggtctttaaa
    22441 tgtacccggc tcgccttcgt cggtgttgca aacgacgtac ttttcgccgg ggaaagaacg
    22501 gggcataaag ctccatttca aaccggtcgg gaagcccgca ccgccacgac cgcgcaagcc
    22561 ggaggttttg acttcgtcaa tcacatcggt ttgcgagatg ttttcggaca ggattttacg
    22621 cagggcggta taaccgccac gtttgacgta ttcgtccaat gtccagcaat cgggattggc
    22681 ggtatccact tggtcaaaaa tcacgcctga ttggtaaata gccatttttg gtgtgcctgt
    22741 ttgttttcgt atcggttgcg gccgctgttt cagacgacct taagatgtct ttgtgtaccg
    22801 gcttgtaacg tcgtctgaaa taaaatctag tttatcaaat cgatattttc agacgacctt
    22861 taatcgtttt tgttggatct cgaccacttg cttgtctgcc gtcattcccg cgcaggcggg
    22921 aatccatcct caatggtaag caatgtctta ttaaattcag aaaccgaatc ttaccggtgg
    22981 attcccgcct gcgcgggaat gacggcattt cggtatttca gtagggcgga ttcttaaatc
    23041 cgacattttg cctttttacc cactctgtcg gatacaagta tccgacctac gtttaaatcg
    23101 tcgtttcaga cgacctactc caactccgcc agtttcttct caatcgcctc ttcagtcata
    23161 aagctgcaca ttttatgatt attcaacaac ataacaggag catcgccgca cgcccccatg
    23221 cattcgcctt cgacaagggt aaatttgccg tcaggcgtgg tttcgccgta gctgataccg
    23281 agtttttgtt tgaggtattc gccggttgcc ataccgccgc gcagggcgca gggcaggttg
    23341 gtacaaacgg tcagtttgta tttgccgaca ggctcaaggt cgtacatatt gtagaaggta
    23401 gcgacttcgt aggcttgtgc aggcgagatg ccgatatagt ctgcaacaaa ggcgatggtt
    23461 tcgggggcga gccagccttt ttcggtttgt gcaatacgca atgcgcccat aatggcagag
    23521 cggcgttggt cggcaggata ttttgccaac tcaatatcaa tttgttttaa ggattctgcg
    23581 gataacatta tcggtcaacc tccccgaata cgatgtcctg cgtaccgatg atggcaacga
    23641 cgtcggcgag catgtggcct tttgccattt cgtccatgcc ttgcagatgg gcgaagccgg
    23701 gtgcgcggat tttcaggcgg tagggtttgt ttgcgccgtc tgaaatgatg taaacgccga
    23761 actcgccttt cggatgttcg acagcggtgt aggtctcgcc ctcgggaacg tgcataccct
    23821 cggtaaagag tttgaaatgg tgaatcaggt cttccatacc tgttttcatt tcggtacgtt
    23881 tgggcggagc aaatttgtgg tttgtggtaa tgaccggacc cggattgaca cgcaaccact
    23941 cggaacattg tttgatgatg cgtacggatt gacgcatttc ttccatacgg cagaggtagc
    24001 ggtcgtagca gtcgccgttc acgccgacag ggatgtcgaa atccattttg tcgtacactt
    24061 cgtaaggctg tgtcttacgc acgtcccatt ccacgcccga accgcgcaac atcacgccgg
    24121 taaagccttt ttgcatggca cgttcggggg agacgacgcc gatgccgacg gtacgctgtt
    24181 tccaaatacg gttgtcggtc aggagggttt cgagtgtgtc gatgtttttc gggaaacgtt
    24241 cgcagaaggc atcgataaag tcgagcatcg tgccttcgcg ggattcgttg agctgcttca
    24301 atactttggc attgcggaat ttgctgccct cgtatttggg catgaagtcg ggcaggtcgc
    24361 ggtaaacgcc gccgggacgg aagtaggctg cgtgcatacg tgcgccggac acggcttcgt
    24421 acaagtccat caactcttcg cggtcgcgga aggcataaag gatggcggtc atcgcaccaa
    24481 tgtcgaatgc gtgcgaaccg atgcccatca aatgattgag gatgcgcgtt acttcggcga
    24541 acatcacgcg gatatattgg gcgcggatag gtacatcgat accggcaagt ttttctactg
    24601 ccaagcaata cgcctgctcg ttgaccatca tggaaacata gtccaagcga tccatatagg
    24661 gcagggcttg cagataggtt ttggtttcag ccagtttttc agtacctcgg tgcaggaggc
    24721 cgatatgcgg gtcggcacgg acgatttgtt cgccgtccag ctccaaaatc atacgcaata
    24781 cgccgtgcgc cgcagggtgt tgcgggccga agttgatggt gtagtttctt aatttattgg
    24841 ccaccgtagt tctcctcacg gacgatacgc ggcgtgatct cgcgcggctc gatggtaaca
    24901 ggttggtaaa tcacgcgttt ttgctcttcg tcgtaacgca tttccacata gccggaaatc
    24961 gggaagtctt tgcggaacgg atgtccgacg aaaccgtaat cggtcaggat gcggcgtagg
    25021 tccgggtggt tgttgaacat gatgccgtac atatcgaagg cttcgcgttc gtaccaatcc
    25081 gcgctgttgt aaatatcgac tacggattcg actacaggga agtcgtcgtc tgaaacccag
    25141 acgcgtacgc ggatgcgttg attgttttta acggaaagca actgactgac gacggcaaag
    25201 cgtttgccct gccatacttc gtttttgtaa gtgctgtaat cgacaccgca caaatcgacc
    25261 agaagctcga aatgcagctc ttcatgatcg cgcaatgcgg tcatgactga aatatagtgt
    25321 tcgggcagac actcgacggt aatctcgccc aaagctgaaa tgactttgcc tgcctgattg
    25381 cccaaaacgc ggctgacggt ttcgtataag ttttgaatgc ttgccatatc ttcctcctct
    25441 tactcgtcac gcgcgatggt ggaagtgcgc ttgatttttt gttggagctg aatcaggccg
    25501 taaatcaggg cttccgcagt cggcggacaa cccggcacat aaacgtctac cggcacgacg
    25561 cggtcggcac cgcgcacaac ggaataagaa tagtgataat agccgccgcc gttggcacac
    25621 gagcccatag acaataccca gcgtggctcg gcgagctggt cgtatacacg gcgcagggct
    25681 ggcgccattt tgttggtcag cgtacctgcc acaatcatca ggtcggcctg acgtggggac
    25741 ggacggaaaa tgataccgaa acggtcaagg tcataacgcg ccatacccgc atgcatcatt
    25801 tccacggcgc agcaggccaa gccgaaagta accggccaca acgaaccggt acgcatatag
    25861 ttcaacaccg tatccgcgct ggtggtgatg aaaccttttt tcaaaacgcc ttctattccc
    25921 attccagcgc accttttttc cattcgtaaa caaagcctac cgtcagaacg acgataaaca
    25981 ccaacataga ccagaagccg tacgcgccca aatctttgaa cacaactgcc cacggcaaca
    26041 tgaacgcgac ctccaaatca aacaggatga agaggatggc gacgaggtaa tagcgcacgt
    26101 caaacttcat cctcgcattt tcaaaggctt cgaaaccgca ttcgtaaggc gcgtcttttt
    26161 cggcatagtg gcgtttcggg cctaaaatcg tgccgagcag gataaacagt acgccggccg
    26221 cgaggccgac gaggataaag acaaagacgg gaaagtaagc ggacaacatg gttacaccca
    26281 aatccgttaa caaaatttct acaataattt cgtattttag cgaatttcaa aaaccattaa
    26341 aaggtaaata tcggcaaaac gcccaaaaaa acccaataaa tacaatcatg ttatgataac
    26401 gattcttatt tgattttata aggtcatata aatttttaca ccgtaattcg tg
//
LOCUS       DAXHPG010000008        26287 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXHPG010000008 DAXHPG010000000
VERSION     DAXHPG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.008"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(131..466)
                     /locus_tag="ACFM6F_000247"
     CDS             complement(131..466)
                     /locus_tag="ACFM6F_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6163740.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            595..1500
                     /locus_tag="ACFM6F_000248"
     CDS             595..1500
                     /locus_tag="ACFM6F_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFC6163741.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGNGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1562..2050)
                     /locus_tag="ACFM6F_000249"
     CDS             complement(1562..2050)
                     /locus_tag="ACFM6F_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6163742.1"
                     /translation="MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2197..3099
                     /locus_tag="ACFM6F_000250"
     CDS             2197..3099
                     /locus_tag="ACFM6F_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6163743.1"
                     /translation="MDTAKKDILGSGWMLVAAVCFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3140..4270)
                     /locus_tag="ACFM6F_000251"
     CDS             complement(3140..4270)
                     /locus_tag="ACFM6F_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6163744.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4475..7444
                     /gene="alaS"
                     /locus_tag="ACFM6F_000252"
                     /pseudo
     CDS             4475..7444
                     /gene="alaS"
                     /locus_tag="ACFM6F_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
     gene            complement(7532..8554)
                     /locus_tag="ACFM6F_000253"
     CDS             complement(7532..8554)
                     /locus_tag="ACFM6F_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6163745.1"
                     /translation="MLTKKEFEKFLDEVCTVLRQEAKNAPFKSQDIFENRVRALCIAK
                     IPAYADIVINPEPVPQIFPDVPIGEYGVEVKFTLKDTWRSVANSIQEKNKADGVQHIY
                     VIFGKMGGIPDVKWQLYEDSVIHVRTSHVPRFEVEIESDRPSLFEGFGISYAEFADLD
                     MHEKMEYIRKYARNRLKEGERLWWIEDIDAVDSHDLPLEVRLYTSLPQEEKIRLRAEA
                     ALVSPRIVCSGRAKHKYDNAVLYLITYRGVLCHQARDLFSAGSVANSAEDYRGGNYVE
                     KSLKLIEHEIEKAALEMDDALIVEYWGESVPTEKRLEYWIKMLDDLADGWIPSESLFN
                     GRYKAK"
     gene            complement(8608..9759)
                     /locus_tag="ACFM6F_000254"
     CDS             complement(8608..9759)
                     /locus_tag="ACFM6F_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6163746.1"
                     /translation="MTEFARKNWNKLAGDAIKAKMHEHGIGFIQLSELFLEQGESETA
                     NAICNKVNRGTFSHAFYLKCMELMTKQEKQEDREKKYKIISLFSGCGGMDLGFLGGFE
                     FLNRTYDKHDFDIIWANEIDEHAVRTYRHNLGNHIVHGDIWQHLDEIPTSADIIIGGF
                     PCQDISINGKGAGISGSRSGLYKAMLSAVERVRPKIFVAENVKGLLMKHHRKDLETVL
                     SDFRNLGYQVDYYLLNAADYGVPQTRERVIIVGRLPEIPEIQIEKTNTSNSYVTARQA
                     IGDLVDKVEDEIANHIWSRAEKSPDQGSRKLDAERAGYTIRAECHGNIQWHYELPRRI
                     SMREAARIQSFPDNFIFQAKLRATERQIGNAVPPVLGWHIAAAVSKALK"
     gene            10382..10612
                     /locus_tag="ACFM6F_000255"
     CDS             10382..10612
                     /locus_tag="ACFM6F_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163747.1"
                     /translation="MFKTLKSIWQGLTQKGKIFPYQLAFTLLIPLRNWALSPQQITQR
                     LHFVPHHRICCGAGYFSCVGCASAHRFLIFAC"
     gene            10853..11797
                     /gene="tehA"
                     /locus_tag="ACFM6F_000256"
     CDS             10853..11797
                     /gene="tehA"
                     /locus_tag="ACFM6F_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFC6163748.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALAAESL
                     LAVASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYVGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GILSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRG"
     gene            complement(11871..12554)
                     /locus_tag="ACFM6F_000257"
     CDS             complement(11871..12554)
                     /locus_tag="ACFM6F_000257"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC6163749.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVIPDGENLKVTLDRVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVLEKFYL"
     gene            12852..15155
                     /gene="parC"
                     /locus_tag="ACFM6F_000258"
     CDS             12852..15155
                     /gene="parC"
                     /locus_tag="ACFM6F_000258"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC6163750.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNSAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESLTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            15176..16767
                     /locus_tag="ACFM6F_000259"
                     /pseudo
     CDS             15176..16767
                     /locus_tag="ACFM6F_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
     gene            16760..17227
                     /locus_tag="ACFM6F_000260"
     CDS             16760..17227
                     /locus_tag="ACFM6F_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6163751.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQADKRLGISFRSMRYRMERLNIG"
     gene            17331..18083
                     /locus_tag="ACFM6F_000261"
     CDS             17331..18083
                     /locus_tag="ACFM6F_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6163752.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLTEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAKPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEAVLLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYEADKG"
     gene            complement(18468..18674)
                     /locus_tag="ACFM6F_000262"
     CDS             complement(18468..18674)
                     /locus_tag="ACFM6F_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC6163753.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(18761..19930)
                     /gene="metZ"
                     /locus_tag="ACFM6F_000263"
     CDS             complement(18761..19930)
                     /gene="metZ"
                     /locus_tag="ACFM6F_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC6163754.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VVSSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGAILVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            complement(20075..20836)
                     /locus_tag="ACFM6F_000264"
     CDS             complement(20075..20836)
                     /locus_tag="ACFM6F_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163755.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKVVPTYAPLSRPSETGTDASVP
                     KLDDASRNRVLKAYLKKWQEEHNAAVADCGEEIETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDTKKDSVEFETGQIEGGENADEAESGTNEPAVSENGENDAEETLMPV
                     QLNILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            20936..21187
                     /locus_tag="ACFM6F_000265"
     CDS             20936..21187
                     /locus_tag="ACFM6F_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163756.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEVA
                     LESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            21239..22045
                     /locus_tag="ACFM6F_000266"
     CDS             21239..22045
                     /locus_tag="ACFM6F_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6163757.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDEQAAK"
     gene            22352..23875
                     /locus_tag="ACFM6F_000267"
     CDS             22352..23875
                     /locus_tag="ACFM6F_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFC6163758.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKSIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTGLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            23973..25385
                     /gene="trkA"
                     /locus_tag="ACFM6F_000268"
     CDS             23973..25385
                     /gene="trkA"
                     /locus_tag="ACFM6F_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC6163759.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAETGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            26154..26229
                     /locus_tag="ACFM6F_000269"
     tRNA            26154..26229
                     /locus_tag="ACFM6F_000269"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26188..26190,aa:Glu,seq:ttc)
BASE COUNT         6400 a         7361 c         6446 g         6080 t
ORIGIN      
        1 cgcttacccc ttgtttctca aatgccgtct gaaaaaacgg ctgcatatgt tgtctacaaa
       61 atatcgggaa gcataacaga caaaatgccg tctgaaatat tttcagacgg catttcttat
      121 ccgaaacgga ttatttttgc gtttcaaccg cttccaatgc acgcagggcg taagtgtaag
      181 cggcaccggc attcagggcg atggcggttg ccaatgcgcc tgcgatttcg ctgtcggtcg
      241 caccggcttt ggcggcagcg gcggcgtgaa cgctgatgca gctctcacaa cgtgtggtaa
      301 tggcaacggc gatggcaatc agttcgcgcg ttttggcatc aagtgcttcg gcggcggcgg
      361 cctgttccaa tgcgccgtag gcctgcagca ttttaggatg cgccttaccc agctcgccga
      421 acgatttttt aaccaatgcg gtatgttctt tccaatcttt aaacattttc ttttcctttc
      481 tcttgcgttt aaccctgata cgcgcttgcg tatctgtttt cgatgtgcgt attattgcaa
      541 ttattcagtt gtgtttctcg tttaatcatc tcattttatg gttcaaaaag atttatggac
      601 attctggaca aactggtcga tttcgcccaa ttgacgggca gtgtggatgt gcagtgcctt
      661 ttgggcggac aatggtcggt acggcatgaa accttgcaac gcgaaggact ggtacacatt
      721 gttacatcgg gcaacggcta tctctgcatc gacggcgaaa cttccccgcg tccggtcagt
      781 acaggggata ttgtattttt cccgcgcggc ttgggtcatg tgttgagcca cgacggaaaa
      841 tgcggagaaa gtttacaacc ggatatgcgg cagcacggtg cgtttacggt caaacagtgc
      901 ggaaacggac aggatatgag cctgttttgc gcccgtttcc gctacgacac ccacgccgat
      961 ttgatgaacg ggctgcctga aaccgttttt ctgaacattg cccatccgag tttacagtat
     1021 gtggtttcaa tgctgcaact ggaaagcaaa aaacctttga cggggacggt ttccatggtc
     1081 aacgcattgt cgtccgtcct gctggtgctt atcctgcgcg cctatctcga acaggataag
     1141 gatgtcgaac tctcgggcgt attgaaaggt tggcaggaca aacgtttggg acatttaatc
     1201 caaaaggtga tagacaaacc ggaagacgaa tggaatgtcg acaaaatggt ggcggctgcc
     1261 aatatgtcgc gcgcgcaact gatgcgccgt ttcaaaagcc gggtcggact cagcccgcac
     1321 gcctttgtga accatatccg cctgcaaaaa ggcgcgttgc tgctgaaaaa aaacccggat
     1381 tcggttttgt cggtcgcact gtcggtaggc tttcagtcgg aaacgcactt cggcaaggcg
     1441 ttcaaacggc aatatcacgt ttcgccgggt caataccgga aagaaggcgg gcaaaaataa
     1501 atcggggctt caaacgcaaa tgccgtctga aaaggctttc atacagcatt tgcgtaccgc
     1561 gtcatttcaa gggctgcatc ttcatcactt ccatcaaaaa gttggtaaat gcggggttgt
     1621 tgggtttgac atccatattt ttccaacgct gctgccagcc gcgcaaggca ttctggatat
     1681 acagcttgga ctgttccgta ttgattgcgc cccgctggct gtctatcgcc gaacgcaggt
     1741 agatttcata catactgtca tcgacggcat tgcgtccgac caggcgtttt ctgaagttgt
     1801 tcagatattg cgccgcctga accttggtca ttttaccgat acccacctga tagcccaagc
     1861 gcgtcgcttc atcgctgatt ttggcaacat ccgtccaatg cgaagaggca aggcggaaac
     1921 cttttgcagg tgcttccgtt ttgacggtat tgataggatt cacggggatt tccgtcaatg
     1981 tgggcacata aatagactgg cagccggaaa gaactgccgc aatggaaaga gggataaggt
     2041 attttttcat gcccccatta taatcaagtt tgccttgaga aaacaaattg ttcggcaaga
     2101 aaaataaaat ttcggcatca gaagcaggca aaaacacatt ccacaagcct tgccgcaagg
     2161 tttacaatcc gaccgtcctt atcgcaacga ccgtttatgg ataccgcaaa aaaagacatt
     2221 ttaggatcgg gctggatgct ggtggcggcg gtctgcttca ccattatgaa cgtattgatt
     2281 aaagaggcat cggcaaaatt tgccctcggc agcggcgaat tggtcttttg gcgcatgctg
     2341 ttttcaaccg ttgcgctcgg ggctgccgcc gtattgcgtc gggacacctt ccgcacgccc
     2401 cattggaaaa accacttaaa ccgcagtatg gtcgggacgg gggcgatgct gctgctgttt
     2461 tacgcggtaa cgcatctgcc tttggccacc ggcgttaccc tgagttacac ctcgtcgatt
     2521 tttttggcgg tattttcctt cctgattttg aaagaacgga tttccgttta cacgcaggcg
     2581 gtgctgctcc ttggttttgc cggcgtggta ttgctgctta atccctcgtt ccgcagcggt
     2641 caggaaacgg cggcactcgc cgggctggcg ggcggcgcga tgtccggctg ggcgtatttg
     2701 aaagtgcgcg aactgtcttt ggcgggcgaa cccggctggc gcgtcgtgtt ttacctttcc
     2761 gtgacaggtg tggcgatgtc gtcggtttgg gcgacgctga ccggctggca caccctgtcc
     2821 tttccatcgg cagtttatct gtcgtgcatc ggcgtgtccg cgctgattgc ccaactgtcg
     2881 atgacgcgcg cctacaaagt cggcgacaaa ttcacggttg cctcgctttc ctatatgacc
     2941 gtcgtttttt ccgccctgtc tgccgcattt tttctgggcg aagagctttt ctggcaggaa
     3001 atactcggta tgtgcatcat catcctcagc ggtattttga gcagcatccg ccccactgcc
     3061 ttcaaacagc ggctgcaatc cctgttccgc caaagataaa aaatgccgtc cgaacatcct
     3121 tcagacggca tatcgggctt tatttccccg ccttcacatc ctgccactgg cgcaccataa
     3181 acttcaatgc cgccggctgg ataggcacca tgataaagct gtttttcaaa tcctcctcgg
     3241 ttgggaaaat cgtattgtcg tttttaaatt cgtcttccat cagctcacgc gccggcttgc
     3301 tcgaaggcgc ataagtaacg aaattgccgt ttttcgccga cacttccgga tcgaggaagt
     3361 cgttgatgta tttgtgcgcg ttggcgacgt ttttcgcatc tttcggaatc acgaaagaat
     3421 ccacccaaat ccccacgccc tctttgggca tcatcacgcg gattttttcc ttgccgcccg
     3481 cttcttcggc acggcgtttg gcgatgttca aatcgccgcc gaaaccgatt gttacgcagg
     3541 tatcgccgcg cgccaaatca tcgataaagc cggacgaagt aaagcgtttg atattggggc
     3601 ggtttttctt gagtagggcg gttgcctccc tgatgtcttc cgtattgctg ctgttcgggt
     3661 ttttacccaa atagttcaac accataggat agatttccgc cgcgctgtcc aaatagctga
     3721 tgccgcattg cttgagtttg gacgtgtatt cggggtcgaa caccaaatcc cactggttgt
     3781 ccggcagctt gtccgtaccc aaagcctttt tcacgcgttc ggtattgatg gcgaaggtat
     3841 ttgtccccca ataaaacggc acggcgtatt cgtggccggg atcgaccccg tccatcagcc
     3901 tcatcatttc agggttgagg tgtttataat tgggaatcag cgacttatcg attttctgat
     3961 acgcacctgc cttaatctgc ctgcccacaa acgcattgga cggcgcgaca atgtcgtaac
     4021 cggacttgcc tgtcagcacc ttgctttcca gcgtttcatc gctgtcgtac acatcataag
     4081 taaccttgat gccgtttttc ttttcaaaat cggcaacggt ttccggatcg acatattccg
     4141 accagttgta aattttcaat acgttttggt tttccgccgg tgccggtttt tcggcaggcg
     4201 gtttgtccga accgccgcac gctgcaagca gcaaagcagt caggacggcc aggggcagat
     4261 gtttggtcat tatcattcct tgcatatcgg gttggagaaa gcggccatta tagccgatat
     4321 tggcaacagg gcttcagacg gcattcaaaa tcccgccaca ctcttccgaa aaccgccgct
     4381 tccatagcta gaaacaggga tttgcggtaa gataccgccg ttcgttttcc ctgcttttac
     4441 catgacaaga catttgagag acattgaaaa aattatgaaa acctccgaac tgcgccaaaa
     4501 attcctaaaa ttttttgaaa ccaaaggcca caccgtcgtc cgctcttcca gcctcgtgcc
     4561 gcacgacgac ccgaccctgc tgtttaccaa cgcgggcatg aaccagttta aagacgtatt
     4621 cttaggtttc gacaaacgcc cgtacagccg cgccaccacc gcgcaaaaat gcgtacgcgc
     4681 aggcggcaag cacaacgact tggaaaacgt cggctacacc gcccgccacc acaccttctt
     4741 tgaaatgatg ggcaacttct ccttcggcga ctacttcaaa cgcgacgcca tccatttcgc
     4801 ttgggagttt ctgacttccc ccgaatggct caacatccct aaagacaaac tgttggcgac
     4861 cgtttacgca gaagacgacg aagcctacaa catctggttg aacgaaatcg gtatgccgtc
     4921 tgaacgcatc gtccgcatcg gcgacaacaa aggcgcgaaa tacgcgtctg ataatttttg
     4981 gcaaatgggc gacaccggcc cttgcggccc ctgctccgaa attttctacg accacggcga
     5041 agaaatttgg ggcggcattc ccggcagccc cgaagaagac ggcgaccgct ggatcgaaat
     5101 ttggaactgc gtatttatgc agttcaaccg cgacgaacaa ggcaatatga atccgctgcc
     5161 caagccgtcc gtcgataccg gcatgggttt ggagcgcatg gcggcagtaa tgcaacacgt
     5221 ccacagcaac tacgaaatcg acttgttcca agacctgctc aaagccgttg cccgcgaaac
     5281 cggcgcagct ttcagcatgg aagaaccaag cctaaaagtc atcgccgacc acatccgctc
     5341 ctgctcattc ctgattgcag acggcgtatt gccttccaac gaaggccgcg gctacgtatt
     5401 gcgccgcatt atccgccgcg ccgtgcgcca cggttacaaa ctgggtcaga aacaagcatt
     5461 tttctacaag ctcgtgcctg atttggtgaa agcgatgggc gacgcgtatc ccgagttgaa
     5521 agagaagcag acgcaaatta tggaagccct gcgcgcggaa gaaagccgct tcggcgaaac
     5581 gctggaaaaa ggcatgggct tgttcaacca agtctataat gaattgaaat tcaatcaaat
     5641 ttttgatctt ctggcgaaag aatttaaggt tacacctgtt gaagatattg atcttgatat
     5701 gtttagtaca agaaattttg taggaagaag tgctgaaatt ccttatatat caaatcaaca
     5761 ccctaacgtg gattatcccg ctcctatggg gaacgtaaat gaaaaaattt atgctatgcc
     5821 agtgagatta acagatggaa gaattggtat taatattttt cctactgatg aaactgcacg
     5881 cctaatcctt agcaatgatt ggaatagttt gcgcttctat tctttttgta attcgttgat
     5941 tacttattat caacagaaag gtcaattaga aacattaaaa ttgaacggcg aacacatctt
     6001 caaactctac gacacctacg gtttccccta cgacctgact gccgacatct gccgcgaacg
     6061 caatatcgaa ctggacgaag caggcttcga gcgcgaaatg gaagcccaac gcacacgcgc
     6121 acgcgccgca caaagcttca aagccaacgc ccaactgcct tacgacggtc aagacaccga
     6181 gtttaaaggt tatagcgaac gccaaaccga atccaaagtc ctcgccctct acaaagacgg
     6241 cgagcaagtc aacgaattga acgaaggcga cgaaggtgcc atcgttatcg actttacccc
     6301 gttctatgca gaatccggcg gccaagtcgg cgacgtcggc tatatctttg caggcgaaaa
     6361 ccgctttgaa gtacgcgata cccaaaaaat caaagcagcc gtattcggcc aattcggcgt
     6421 acaaacttca ggccgtctga aagtcggcga cagcgttacc gccaaagtgg acgacgaaat
     6481 ccgcaatgcc aatatgcgca accacagcgc gacccacctg atgcacaaag ccctgcgcga
     6541 cgtattgggc ggacacgtcg aacaaaaagg ctctttagtt accgccgaat ccacccgttt
     6601 cgacatttcc catccccaag cggtcactgc cgaagaaatc gccgaagtcg aacgccgcgt
     6661 caacgaagcc gttttggcga acgttgccgt caatgcagcc attatgagca tggaagacgc
     6721 gcaaaaaacc ggcgcgatga tgctcttcgg cgaaaaatac ggcgaagaag tgcgcgtact
     6781 gcaaatgggc ggtttctcta ccgaattgtg cggcggcaca cacgtttcac gcaccggcga
     6841 catcggcctc ttcaaaatca tcagcgaagg cggtattgcc gcaggcgtgc gccgtatcga
     6901 agccatcacc ggcctgaacg cactcaaatg ggcgcaagag caagagcgtt tggtgaaaga
     6961 cattattgcc gaaaccaaag cccaaaccga aaaagacgta ctggcaaaaa tccaagcagg
     7021 cgcggcacac gccaaagcat tggaaaaaga attggcacgc gccaaagccg aactcgccgt
     7081 ccacgcaggc gccaaactct tggacgatgc aaaagacttg ggcgcagcca aactcgttgc
     7141 cgcccaaatc gaagccgacg cagccgccct gcgcgaaatc gttaccgatt taaccggtaa
     7201 atccgacaac gccgtgattc ttttagcggc agtaaacgac ggcaaagtct ccctgtgcgc
     7261 cggcgtatcc aaaccgttga ccggcaaagt gaaagcaggc gatctggtta aatttgcagc
     7321 cgaacaagtc ggcggcaaag gaggcggcag accagatttg gcacaggcag ggggcacgga
     7381 tgcaggcaaa ttgcccgccg tgttggatag cgtgaaagac tgggtcggcg cgaagctggc
     7441 ttgatggaga aaggcagcct gaaagggatt caggctgcct tttgtgttaa agatgccgtc
     7501 tgaaagatat tttcagacgg cctctgtcag tttatttggc tttataccta ccattgaata
     7561 gcgattcaga aggtatccag ccatctgcca aatcatccaa catcttaatc caatactcta
     7621 ggcgtttttc tgtcgggacg ctttctcccc aatattcgac aattagagca tcatccattt
     7681 ccaaagcagc tttttcaatt tcatgctcaa tcaattttaa agatttttct acatagtttc
     7741 cgccacggta gtcttccgcc gagttggcaa cgctgcccgc actgaataag tcccgcgcct
     7801 gatgacaaag cacaccgcga taggtaatca gatacaggac ggcattatcg tatttgtgct
     7861 tcgccctgcc tgagcagaca atacgggggg agactaatgc tgcttcagct cggaggcgga
     7921 ttttttcttc ctgcggcagg gaagtgtaaa ggcgcacttc caaaggcaga tcatggctgt
     7981 ctactgcgtc aatgtcttct atccaccata accgctcgcc ttctttcagc ctgttacggg
     8041 catattttcg gatatattcc attttttcat gcatatccaa atcggcaaat tcagcatagg
     8101 aaataccgaa accctcaaac agagacggtc gatcagattc tatttctacc tcaaagcgcg
     8161 gaacatgcga tgtgcggaca tgaattacgc tatcttcgta aagttgccat ttcacatcgg
     8221 gaatgccccc catttttccg aatatcacat agatatgctg tacaccgtca gctttgtttt
     8281 tctcttggat actgtttgct acgcttcgcc aagtatcttt tagcgtgaat ttgacttcta
     8341 cgccatattc ccctattgga acatcgggga aaatttgcgg cacaggttca ggattgataa
     8401 ctatgtcagc ataagcggga atttttgcaa tgcacagcgc acgaactctg ttttcaaaaa
     8461 tatcttggga tttgaaaggt gcgttttttg cctcttgcct caatacggtg caaacctcgt
     8521 ctaaaaactt ctcaaactcc ttttttgtca gcataatgca ttaccactta aaaatataaa
     8581 gtagtaattt tatcttattt aacagggtta tttaagtgcc ttactgacag cggcggcgat
     8641 gtgccaccct aaaacaggag ggacggcgtt gccaatttgc cgttctgtgg cacgcaattt
     8701 tgcctggaaa ataaaattgt cgggaaacga ttggatgcgc gccgcctcac gcatggagat
     8761 gcggcggggt aattcgtaat gccactgtat gtttccatgg cattctgccc taatagtgta
     8821 gcccgccctt tcggcatcca attttcgact accttgatcg gggctttttt ctgctctact
     8881 ccaaatatgg ttggcaattt cgtcttctac tttgtcgacc aaatcgccta tagcctgtct
     8941 ggctgttaca tagctgttgc tggtatttgt tttttcaatc tgtatttcgg gaatttcagg
     9001 tagccttcca acgataataa ctcgctcccg agtctgtgga acaccgtaat cggctgcatt
     9061 cagcaagtag taatctacct gataacccaa attgcgaaaa tctgaaagca ctgtttccaa
     9121 atctttccga tgatgcttca tcagtagccc tttgacattt tcagcaacaa atattttggg
     9181 gcgaacgcgt tcaacagcag acaacatggc tttatacagc ccgctgcggc tgcctgaaat
     9241 acctgcacct ttaccattaa tagaaatatc ctgacaagga aagccaccga taatgatgtc
     9301 tgcgctagtg ggtatttcat ccaaatgctg ccaaatatca ccatgtacaa tgtgatttcc
     9361 taagttgtgg cggtaggttc taacagcgtg ttcgtcgatt tcgttcgccc aaataatatc
     9421 aaaatcatgt ttgtcataag tacggtttaa aaactcaaag ccccccagaa aacccaaatc
     9481 cattccgcca caaccgctaa ataaagaaat aattttatat tttttttcgc ggtcttcttg
     9541 cttttcctgt ttggtcatta actccataca tttaagataa aaagcatggg aaaaagtacc
     9601 tctatttact ttgttacaaa ttgcatttgc cgtttcactt tccccttgct ccaagaataa
     9661 ttctgataat tgtataaagc caattccatg ctcatgcatt ttggctttta tggcatctcc
     9721 agctaacttg ttccaatttt ttcgtgcaaa ttcggtcatg gttaatcttt cagtcattca
     9781 taaatgacat tattttagcg ttttttataa tttaagccaa gatttaaaat ctacctttag
     9841 gtttacaggc cgtctgaaaa caaaaatctc gttttcagac ggcctttttc cgctaaaatc
     9901 cccctaccgt ccaacccgaa acaaggagaa ccatcatgca agttcctact tccgccatca
     9961 tcaacggcga atttgccgac aaatacggca aacgcggcag caagccgtag ccagcacccc
    10021 tgtcaaccgc gtgcgtggct agaccacaca ccctaccact gcgggcgacg caaaccttaa
    10081 gattgaggtg tagggtgtgt gcggtacgca cgcacgcggt ttttgttact accgaatgat
    10141 tccaaaccat gccgtaatcc agtcagcagc aaactcagct tgggtcatgt accaacaaaa
    10201 ctcaatatca aaggccgtct gaagaataat tttcagacgg cttctctcta tcaaaagcag
    10261 cctgcacatt caaaaatcga agtgcaggct gctttttgtg atgattggaa aaaacacaca
    10321 ctatttttat actgtatatt gtgcaggtac aaagtaaaac gaattaccca aagaaattcc
    10381 catgtttaag acgctcaaaa gcatttggca gggacttacg caaaaaggca aaatcttccc
    10441 gtaccaactg gcattcacgc tgctgattcc tttgcgtaac tgggcgcttt caccgcaaca
    10501 aatcacgcaa cggctgcact ttgttccgca ccaccgaatt tgctgcgggg cgggttattt
    10561 cagttgcgta ggatgtgctt cagcccaccg tttcctcatt tttgcttgtt aattggtggg
    10621 ctgaagccca cactacggtt aatacggtta ctgattcgtt tccccctgca tccgaatcac
    10681 gccgcacacc gcaaaaaacg cgcgcccgat gcgcaaccgc ttccgacatt tggcgcaatg
    10741 agacaaaacg gcaatatgcc gatacggata cgtcatcctc cgcctgcctg ttttttatat
    10801 catactcaat tttatctgtg cgttacgacg gcatgccgtc tgaagggtgt ttatgtctgc
    10861 atctaagaaa tttccgattc ctttgagcta tttcagcatc gcgctgggct tgtttgcctt
    10921 ggggctgtcg tggcgttacg gcgcgtctgt cgggctgctc cccgccttgg ccgccgaatc
    10981 gctgcttgcg gtggcttcgg ttgtctggct cttgctggtg gcggcatacc tgatcaaaat
    11041 gtttgcgtac cgaaacgatt ttttgtctga tttgcgcgac ttggtgcaat gctgcttcat
    11101 cagcgcgatt ccgattaccg ctatgctgga gggactcgcg ctgaagccct atcaggcagg
    11161 cgcggcggca gtcctgattt atgtcggcgt tgccggacag ttggcgtttt cgatgtatcg
    11221 ggcggccggt ctgtggcgcg gcctgcattc cttggaggcg acgacgccga ttatttatct
    11281 gcctacggtt gcgacaaact ttgtcagcgc gtcatctctg gccgcgttgg ggcatcatga
    11341 ttatgcagct ttgtttttcg gcgcgggtat gttttcctgg ctgagcttgg aagcctccat
    11401 cttgggcagg ctgcgcacgg cggcaccggt cggcacggcg gcgcgcggcg tggtcggcat
    11461 ccagcttgcg cccgcctttg tcggctgcgg cgcgtatttt gccgtcggcg gtaaagtcga
    11521 cggttttgcg ttggcattaa tcggctacgg ctgcctgcag cttttgttct tgctgcgcct
    11581 gacccgctgg ttttgggaag gcggttttac gatgagcttt tggggatttt cattcggttt
    11641 cgcggcaatg gcaggatgcg gtctgcatct ggcggcttct ggcatattgt cgggcttggg
    11701 gctgacgctt gccaccgccg gatcggcagg cgtggcgctg ctgcttgtcg gtacgctgca
    11761 ccggatagcg acggggcgtt tcttggtacg cggctgatgc gttttgccgc cttgtcaaaa
    11821 atgccgtctg aacgcccaag ccttcagacg gcatttttta tttcaaaccc ttacaggtag
    11881 aatttttcga ggactttcag gttgtcatcc aatttgtaca ccagcggctg accggtcggg
    11941 atttccaagc ccatgatgtc ttcgtcggaa ataccctcga tgtgttttgc cagcgcgcgc
    12001 agggagttgc cgtgcgccgc taccaagacg cgtttgccgc tcaaaatcgc gggggcgatt
    12061 tggtcttccc aaaacggcaa tacgcgatcc agcgttactt tcaggttttc gccgtcgggg
    12121 ataacatcgg caggcagatg ggcatagcgg cggtctttgt gtgcggaaaa cgcgtcgtct
    12181 ttgtccaaaa gcggcggcag agtgtcgtag ctgcgccgcc agatgcggac ttgctcgtcg
    12241 ccgtattgtt cggcggtttg ttttttgtcc aaaccttgca gttggccgta gtggcgttcg
    12301 ttcagccgcc acgttttgat ttgcggtacg aacagttggt cggattcttc caaaacgatg
    12361 ttgcaggtct taatcgcgcg ggtcaggacg gatgtgaagg cgatgtcgaa ctcatagccg
    12421 ttttctttca gttttttacc ggcggcggca gcctcggcaa gcccctgctc gctcagcttc
    12481 acgtcgcgcc agcctgtaaa caggtttttc gcgttccatt cgctttgtcc gtggcggata
    12541 aataccagtt ccatatcgtc tccaatgtgt gaaagtagga aagccttatt tataacatat
    12601 tttcacattt cccgtatttg attcagattt agacacggtg cccactatgg tttgccgttt
    12661 tgatttacaa taatgtcctt tgctttacat tccgcataca caatgtttca acacacagga
    12721 cgacacataa agcaccgccc tatgtgttgc cctgatttgg aaggggttgc cccctcccaa
    12781 ataaagtctg attctaccgc cccgaaggac agatgtccaa gtggcggggg ttcaaccgaa
    12841 aaggaaatac gataaatatg caaccgcacg ctccacatac cgattccaat acgctgatgc
    12901 tcggccgata cgccgaacgc gcctatctcg aatacgccat gagcgtggtc aaaggccgcg
    12961 cgctgcctga agtttcagac ggccaaaagc ccgttcagcg gcgcattttg tttgcgatgc
    13021 gcgatatggg tttgacggcg ggagcgaagc cggtgaaatc ggcgcgcgtg gtcggcgaga
    13081 ttttgggtaa ataccacccg cacggcgaca gttccgccta tgaggcgatg gtgcggatgg
    13141 cgcaggattt taccttgcgc tatcccttaa tcgacggcat cggcaacttc ggttcgcgtg
    13201 acggcgacgg ggcggcggcg atgcgttaca ccgaagcgcg gctgacaccg attgcggaat
    13261 tgctgttgtc cgaaatcaat caggggacgg tggatttcgt gccgaactac gacggcgcgt
    13321 ttgacgaacc gctgcacctg cccgcccgcc tgcctatggt cttgctcaac ggtgcgtcag
    13381 gcattgcggt gggtatggcg accgaaatcc catcacacaa tttgaatgaa gtcacacagg
    13441 cggcgattgc actgttgaag aagccgacgc tggaaaccgc cgacctgatg caatacatcc
    13501 ctgctcccga ttttgccgga ggcggtcaaa tcatcacgcc ggcggacgaa ttgcgccgta
    13561 tttacgaaac cggcaagggc agcgtgcgcg tgcgtgcgcg ttacgaaatc gagaagctgg
    13621 cgcgcggaca atggcgcgtc atcgtaaccg agctgccgcc gaacgccaat tccgccaaaa
    13681 tccttgctga aatcgaagaa cagaccaacc cgaaaccgaa agcaggcaaa aagcagctca
    13741 accaagacca gctcaatact aaaaagctga tgctggattt aatcgaccgc gtgcgcgacg
    13801 agtcggacgg cgaacatccc gtgcgccttg tatttgaacc aaaatccagc cgcatcgata
    13861 ccgatacctt catcaacacg ctgatggcgc aaacttcgct ggaaggcaat gtgtccatga
    13921 acttggtgat gatggggctg gacaaccgcc ccgcgcagaa aaacctgaaa acgattttgc
    13981 aggaatggtt ggatttccgc accgttaccg taacacgccg tctgaaattc cgtttgaacc
    14041 aagtggaaaa acggctgcac atcctcgaag gccgtctgaa agtctttctg cacatcgacg
    14101 aagtgattaa agtcatccgc gaatcagatg acccgaaagc cgatttgatg gcggcgttcg
    14161 ggctgaccga aatccaagcc gaagacattt tggaaatccg cctgcgccag ttggcacgtt
    14221 tggaaggttt caaactcgaa aaagaattga acgaattgcg cgaagaacaa ggccgtctga
    14281 acatcctttt gggcgatgaa aacgaaaagc gcaagctgat tatcaaagag atgcaggcgg
    14341 atatgaaaca atacggcgat gcacgccgca cgctggtgga agaggccgga cgcgccgtgc
    14401 tgacacaaac caccgccgac gaacccatca cactgattct gtcggaaaaa ggctggatac
    14461 gcagccgtgc cggacacaat ctcgatttga gccaaaccgc gttcaaagaa ggcgaccgcc
    14521 tcaaacaaac ccttgaaggc agaacggttt tacccgtcgt catcctcgat tcattgggca
    14581 gaacctatac actcgatgcc gccgaaattc ccggcggacg cggcgacggc gtgccggttt
    14641 catccttgat cgagttgcaa aacagcgcga aacccgttgc gatgttgaca ggattgccgg
    14701 aacaacatta tttattatca agcagcggcg gctacggctt catcgccaag ttggctgata
    14761 tggtcggacg cgtaaaagcg ggcaaagtgg tgatgaccac agacagtggc gaaaccgtcc
    14821 tgccgccgat tgccgtctat gcctcctcgt tcatcaaccc cgactgcaaa atcattgccg
    14881 ccaccagtca aaaccgcgcc ctcgccttcc ccatcggcga attgaaaatt atggcgaaag
    14941 gcaaagggct gcaaatcatc ggattaaacg ccggcgaatc gctgacgcat accgccgttt
    15001 cttccgagcc ggaaatcctg attgaaagcg aaggcaggcg cggcgcggcg cacaaagacc
    15061 gcatccccgt ctccctgctt gaggcaaaac gcggcaaaaa aggcaaacta ttacccatat
    15121 cgggcagcct gaaacagctt tcttccccca aataaacccg attccacaca taattatggt
    15181 gatttccaac ccccgcgaac ttgaaaaact caaagaccgg attcccaatc tgatcaacat
    15241 catccgcgtc gccatcgttt ttccgctgat gattatgcac atcctcgggc tggaaaccgg
    15301 cagccgtgcg aacctgcacg cttcgtggac ggcgtgggcg ttttatgttt ggctcgccat
    15361 tgcctgctgg ctgattttct tttccatcat ccatccgcat tggcaatggc agtcgctgaa
    15421 aatgccgcgt ttcagcgcgg tggcggacat cacgatgatc ggcgtgctga cctacctgtt
    15481 tggcggcatc gattccggct tcggcatcct gatcctgccc ttcgtcgtct gctcctgcct
    15541 gctcagctac gggcgttacc ccctgctcta ttccagctac gccgccatcc tgctgatatt
    15601 caacgccatt gccgacggcg atatcggcaa atacccgctc atatcggatg cccgaaccgc
    15661 ctcggtaacc ttcatccttg tcgccgcctc ctatctttcc gccatcttca cctcactgtc
    15721 ggtcagatac atcgacagcg ccggccggct ggcacgcgac aaccacctcg cctaccggcg
    15781 catcaaaggc ttgagccaaa ccgtactcaa ccgcgttcag gaagcggtcg tcgtcatcaa
    15841 cgtcgagcat cagaccatac tgttcaataa aaaggcaaaa gacctgctac ccatgcttga
    15901 aatcggacag catactgccc tgttcgaccc tattaccgtt ttatgggaca aggcctcttc
    15961 gcgtactttc gaacgcaata tcgacacgcc cggcctgaac gccggcatcc gcgccgttcc
    16021 ggtcaacaaa gggccggaca agctgctcat cctctacatc cgcccgcaaa gcgaaattca
    16081 ggcagaagcc ctgtccgtca aacttgccgc gctcggacaa ctgaccgcca acctcgccca
    16141 cgaaatccgc aacccgatgt ccgccatccg ccacgccaac gacctgctgc gcgaaaatat
    16201 ggaagcgggg gcggcagatc cgttcaacgc caaattgtgc aaaatcatcg acggcaacgt
    16261 ctgccgtatc gacaaaatgc tcgaagatat ttcctcgcca acaagcgcaa caaaaccgaa
    16321 cgcgaaacca tcggcctgat accgttttgg gaagaattca aacaagagtt cctgctcggc
    16381 catcccgatg ccgccggctg catccgccta gatatgcagg gcaatcacct gactgcctat
    16441 ttcgatcctg cccacctgcg gcaaattatg tggaacctcg ccaacaacgc gtggcggcac
    16501 agcagcaaac gtaacggctc gattgccgtc gtcgcccgtc ctgcacaaaa aaacaccatc
    16561 agcatcctgt ttgccgacga cggcggcggc gttccgcccg aagtgcagga acacttgttc
    16621 gaaccctttt acaccacagc aaaaaacgga accggcttgg gactgtatgt cgcccgcgaa
    16681 ctggcacacg ccaatttcgg cgatttgacc tacctgccgg aagccaaatg tttcgaactc
    16741 acattaccgg aaaaaaccaa tgactgaact gcaacatccc gtcctcgtcg tcgatgacga
    16801 aaccgacatt ctcgacctga tggaaatgac cctgatgaaa atgggcttgc gcgtccatac
    16861 cgcgtcaggc gttgccgaag ccaaaaacaa gctcgacagc caacgctatt cgctcgtcct
    16921 gaccgatatg cgtatgccgg acggctcggg gctagaagtc gtccaacaca tcaacagccg
    16981 cctgctcgat acgccggttg ccgtcatcac cgccttcggc aatgccgatc aggcacagga
    17041 agccctgcgt tgcggcgcgt tcgaccccga taccatgcag atacaggact atctcgacca
    17101 aatcgaacgc gacatcatcg aacaaaccct caaacaaacc gaaggcaacc gcacgcaggc
    17161 cgacaaacgc ttgggcatca gcttccgttc catgcgctac cgtatggaac gcctcaacat
    17221 cggctgacga caaaacggca tccgcaccat ctccgcccac ccgaaaaaat gccgtctgaa
    17281 acggcacggg aaagcgggtt cgccccacgc ccgaacggac acaaaacacc atgaccgaca
    17341 tccttattga caacaccgcc accgaaaccg tccgcaccct gatacgggca ttcccccttg
    17401 tgcccgtttc ccaaccgccc gaacaaggca gctacctcct taccgaacac gataccgtca
    17461 gcctcaggct tgtcggggaa aaaagcagcg tcatcgtcga ctttgcctcg ggagccgcac
    17521 aataccggcg cacaaaaggc ggaggagaac tcatcgccaa agccgtcaac cataccgcca
    17581 aacccaccgt atgggatgca accgcaggat tggggcgcga cagcttcgtc ctcgcctcgc
    17641 tcggactgac cgttaccgcc ttcgagcagc atcccgccgt cgcctgcctg ctttcagacg
    17701 gcatccgccg cgccctcctc aatcccgaaa cgcaagacac cgccgcgcgc atcaacctcc
    17761 atttcggcaa cgccgccgaa cagatgcccg cccttgttca aacacaaggc aaacccgaca
    17821 tcgtctatct cgaccccatg tatcccgaac gccgcaaaag tgccgccgtc aaaaaagaaa
    17881 tggcctactt ccaccggctc gtcggcgagg cgcaagatga ggccgtcctc ctccataccg
    17941 cacgccaaac ggcaaaaaaa cgcgtcgtcg tcaaacgccc ccgcctcggc gaacaccttg
    18001 ccggacaagc ccctgcctac caatacacag gcaaaagcac ccgcttcgac gtttacctgc
    18061 cctacgaagc ggacaaggga taacgcccat aaaacaagac accgaaaaat ttgccgttct
    18121 tatgcaaacg agaaaccggt ttttgcgttt cgactgtttt ggataaatca tcacacctta
    18181 aagtttgtca ttcccacgga agtgggaatc tagaaatgaa aggcaacagg aatttatcgt
    18241 aaatgactga aaccgaacgg actagattcc cgcctgcgcg ggaatgacga tgtaaaatta
    18301 tccgggattc aaaaagacag actttcgcgt ccgtgggaat gactgcggaa agatgatttt
    18361 tattttgtca taaaaatccg caccttaatc agttggcggt taaatcaaac ttttagggtg
    18421 cagattactt tttatgattt cagacggcat tttgacaggc cgcaaaccta tttcggcaat
    18481 accaaaaact taatcagcag ttctttgaat acaaaaccga acacgcccaa gcccaaaacc
    18541 aaaaacaaaa tggcgatgcc gaatttgcct gctttggact ccttgcccaa attccaaacg
    18601 ataaaaccca aaaaaataat caagccggtc aggcagattt tcaacgccca atcggcaaaa
    18661 accgcttcat ccatattttt ttcctattgt tgatgtgtat gccatataag ataagggttt
    18721 cagacggcat ctgctgtcca atgccgtctg aaacacgcaa tcagcgtgcg agtgcctgtt
    18781 tcaaatcgtc aatcaaatcg ccaacatatt ccaaaccgac cgacaggcgc accaatccgg
    18841 ggcggatgtt ggcggcgagt ttttcttcgg gctgcatcct gccgtgcgtg gttgtccacg
    18901 gatgggtgat ggtcgagcgc acgtcgccga ggttggcggt acgggaaaag agttccacgc
    18961 cgtccacgac tttccacgcg gctgcttggt cggcgacttc aaagccgatg acgatgccgc
    19021 cgccgttttg ctgtttgcgg ataagcgccg cctgcggatg gtcgggcaat ccggtgtagt
    19081 acacggcttg aacctgcggc tgcgcttgca gccattgtgc gattttcagg gcgttgtcga
    19141 actgtttttc catacgcagc gacagggttt ccacgccgct caacaactgc cacgcattaa
    19201 acggcgacat cgccagcccg caagagttgc agtaaacggc ggcctgcgcc atcaattctt
    19261 ccgaacccgc caacacgccg cccatcacgc gtccgtgtcc gtctatggct ttggtcgcgg
    19321 aggaaacgga aatatccgcg ccgtgtttca aaggctgcga gccgacgggc gacagcaggc
    19381 tgttgtccac caccaaaatc gcgccgatgc cgtgcgccaa ttccgccaag gcttccaagt
    19441 cggccacttc gcccaaggga ttggacggcg tttccaaaaa cagcagtttg gtgttggctt
    19501 tgacggcggc tttccattcg tttatatcag tcggcgacac gtggctcact tcgatgccga
    19561 atttggtaac gatgttattg ataaagccga cggtcgtgcc gaacaggctg cggctggaaa
    19621 ccacgtggtc gcccgcctgc aaaaaggtga aaaacgccgc ctgaatcgca gacatacccg
    19681 tcgaagtggc gaccgcgcgt tccgcacctt ccaaagcggc gatgcgtttt tcaaaggcgg
    19741 ctgtggtcgg gttggcggta cgggtataag tgaacccttt gatttttttt gaaaacaaat
    19801 cggcagcgtg ttgggcgttg tcccacatga agctgctggt cagaaacaat gcctgattgt
    19861 gttcgcggta ttcggtttgt tctttgccgc cgcgtatggc gagcgtttgc ggatggagtt
    19921 ttttgctcat cggtgattcc tcggttttgt ccgttcggca acggagcgtg cgcccgttgt
    19981 ttaatttgtt aatattttgc gcctgttcca tgaggctttc aagtcggatg agaatgcaaa
    20041 tgccgtctga aacggctttc agacggcatg gcaatcagcg tttgtatttt aactcgtact
    20101 tgatgtcgtt gaggattttg cggacatcgt gttccaacac gtcttcgact accgcccccg
    20161 cctgctcgtg cagcatctgc tggagctgat aggtgaaaac cgccatctgc ttttgcaccg
    20221 ccgttcggat gatgccgttg acggtatcgg tcagatgggg gcgcaggcgt ttgatcagcc
    20281 gttcggtcag ctcctgttcg gacaggcaga acacttcgcg ccggttgacg gctttcgggt
    20341 tcaggatatt gagttggacg ggcatcaacg tttcttccgc atcgttttcc ccgttttccg
    20401 aaaccgccgg ctcattcgtg ccggattctg cctcgtcggc gttttccccg ccttcaatct
    20461 gtccggtttc aaattcgaca ctgtcttttt tggtatcaaa ccggattctc cgccgcgatt
    20521 cgatgtgttt ttccgaaacc gacatttgca gggaagcctg cgcgttgagc cagttttcct
    20581 gaaggacgat catcgggtcg gtttcgattt cttccccgca atcggcaacg gcggcattgt
    20641 gctcctcctg ccatttcttc agatacgcct ttaacacacg gtttcggctt gcatcgtcca
    20701 gtttcggcac agacgcgtcc gttccggttt cagaagggcg ggacagcggc gcgtaagtcg
    20761 gcactacctt catacggcgc gtctgacgca ggttttccaa acgtttttcc caattcggct
    20821 ctttattcgc atccattttc ggcttccggt ccttaatctt tgcaagcaga caaacccgcg
    20881 cccaaagcgc ggtttgatat aatggcgcat tttaacagat tcgcgaggat acatcatggg
    20941 cagcatcgaa cagcgtttgg aatatctgga agaggcgaac gacgtgctgc gtatgcagaa
    21001 ccacgtcctg tccaccgcct tcaaagcctt aatccgcgcc cttcccgccg acaccgccga
    21061 agtcgcgctc gagtccatcc agcttgcttt tgaggacgcc ttggcagaat tgagctatga
    21121 ggacagcccg catacggatt tgttccacga cgttacttat gcgtttttcc gtgaaaaaga
    21181 acgttaattt tatgttaaac tgatttttta ggctttttga ttaccgaaag gaattttgat
    21241 gaatatgaaa aaatggattg ccgccgccct tgcctgttcc gcactcgcgc tgtctgcctg
    21301 cggcggtcag ggcaaagatg ccgccgcgcc cgccgcaaac cccgacaaag tgtaccgcgt
    21361 ggcttccaac gccgagtttg ccccctttga atctttagac tcgaaaggca atgtcgaagg
    21421 tttcgatgtg gatttgatga acgcgatggc gaaggcgggc aattttaaaa tcgaattcaa
    21481 acaccagccg tgggacagcc ttttccccgc cttgaacaac ggcgatgcgg acgttgtgat
    21541 gtcgggcgta accattaccg acgaccgcaa acagtctatg gacttcagcg acccgtattt
    21601 tgaaatcacc caagtcgtcc tcgttccgaa aggcaaaaaa atatcttctt ccgacgattt
    21661 gaaaaacatg aacaaagtcg gcgtggtaac cggctacacg ggcgatttct ccgtatccaa
    21721 actcttgggc aacgacaatc cgaaaatcgc gcgctttgaa aacgttcccc tgattatcaa
    21781 agaactggaa aacggcggct tggattccgt ggtcagcgac agcgcggtca tcgccaatta
    21841 tgtgaaaaac aatccgacca aagggatgga cttcgttacc ctgcccgact tcaccaccga
    21901 acactacggc atcgcggtac gcaaaggcga cgaagcaacc gtcaaaatgc tgaacgatgc
    21961 gttggaaaaa gtacgcgaaa gcggcgaata cgacaagatt tacgccaaat attttgcaaa
    22021 agaagacgaa caggccgcaa aataagcccg cccatccgaa cacaatgccg tctgaagccc
    22081 tttcagacgg cattgttcat caatcggcct acaatgaact gcctgctgat ttctccctac
    22141 cgcaaagcaa caggcaaaga ttacaaatat caaaatccga gtaaaacagt attttattaa
    22201 aacaaattga taatcaagag attagaatta tgtattgtct ttaccgtaca aacgctggca
    22261 ctatttcaac ctgataaaaa acagccttca aaaaggttgc ttaaaacagc agcagacact
    22321 taccgccaca accttgaaaa ggaacacaat catgaccgtc atcaaacagg aagactttat
    22381 ccaaagcatt tgcgatgcct tccaattcat cagctactac catccgaaag actacatcga
    22441 cgcgctttat aaggcgtggc agaaggaaga aaaccccgcc gccaaagacg cgatgacgca
    22501 gattttggtc aacagccgca tgtgtgccga aaacaaccgc cccatctgcc aagataccgg
    22561 tatcgcgacc gtcttcctca aagtcggtat ggatgtgcag tgggatgcgg acatgagcgt
    22621 cgaagaaatg gttaacgaag gcgtgcgccg cgcctacact tgggaaggca acaccctgcg
    22681 cgcctctgtc ctcgccgatc cggccggcaa acgtcaaaac accaaagaca acacccccgc
    22741 cgtcatccat atgagcatcg tgccgggcgg taaagtcgaa gtaacctgcg cggcaaaagg
    22801 cggcggctct gaaaacaaat ccaaactcgc catgctcaat ccttccgaca acatcgtcga
    22861 ttgggtattg aaaagcatcc caaccatggg cgcaggctgg tgtcctcccg gcatcttggg
    22921 catcggcatc ggcggcacgc ccgaaaaagc cgtgctgatg gcaaaagagt ccctgatgag
    22981 ccacatcgac attcaagagt tgcaggaaaa agcagcgtca ggtgcagaat tgtccaccac
    23041 cgaagccctg cgcctcgaac tctttgaaaa agtcaacgcg ctaggcatcg gcgcgcaagg
    23101 cttgggcggt ctgaccaccg tgttggacgt gaaaatcctc gattacccga cccacgccgc
    23161 ctccaaaccg attgccatga ttccgaactg cgccgccacc cgccacgtcg aatttgaatt
    23221 ggacggctca ggccctgtcg aactcacgcc gccgcgcgtc gaagactggc ccgatttgac
    23281 ttacagcccc gacaacggca aacgcgtcga tgtcgacaag ctgaccaaag aagaagtggc
    23341 aagctggaaa accggcgacg tattgctgtt gaacggcaaa atcctcaccg gccgcgatgc
    23401 cgcacacaaa cgcctcgtcg atatgctcaa caaaggcgaa gaattgcccg tcgatttcac
    23461 caaccgcctg atttactacg tcggccccgt cgatccggtc ggcgatgaag tcgtcggtcc
    23521 ggcaggtccg accacagcca cccgcatgga caaattcacc cgccaaatgc tcgaacaaac
    23581 cggcctcttg ggcatgatcg gcaaatccga gcgcggcgtg gccacctgcg aagccatcgc
    23641 cgacaacaaa gccgtgtacc tcatggcagt cggcggcgcg gcgtatctcg tggcaaaagc
    23701 catcaaatct tccaaagtct tggcgttccc cgaattgggc atggaagcca tttacgaatt
    23761 tgaagtcaaa gacatgcccg taaccgtcgc cgtagatagc aaaggcgaat ccatccacgc
    23821 caccgccccg cgcaaatggc aggcgaaaat cggcatcatc cccgtcgaat cttgaggcgc
    23881 catgccgtct gaacacaaaa tctgccttca gacggcattt ccgcccccgg ttgcggtaca
    23941 atccaccatt tcatcactcg gcgacccaca ccgtgaaaat cctcatttta ggcaacggac
    24001 aggtaggttc taccgtcgca caaaaccttg ccgccatacc caacaacgac gtaaccgtta
    24061 tcgacatcga cgaaaaagca ttgcaggaaa caggcagccg cctcgatgtc caaaccgttt
    24121 tcggcaacgg cgcatccccc ttcacattag aacgcgccgg cgcggaagat gccgacttgc
    24181 tgctcgcgct ctcccgcagc gacgaaacca acatcgtcgc ctgcaaagtt gccgccgacc
    24241 tgttcaacat ccccggccgc atcgcgcgcg tccgttccag cgaatacctc gaatacctca
    24301 gccccaagct cgaaaacaac gaaaacggca gcctttccat attcggcata accgaaacca
    24361 tcagccccga acagctcgtt accgaacagc ttgccggcct gatagactgc ccgggcgcat
    24421 tgcaggtttt acgttttgca gacgaccgcg tgcggatggt catcatacag gcgcggcgcg
    24481 gcggactgct tgtcggacgc agcattgccg acatcgccca agatttgccc gacggggccg
    24541 actgccaaat ctgcgccgtt taccgcaaca accgcctcat cgtccccgcg ccgcaaaccg
    24601 tcatcatcga aggcgacgaa atcctatttg ccgccgccgc cgaaaacatc ggcgcggtca
    24661 tacccgaatt gcgccccaaa gaaaccagca cccgccgcat catgattgcc ggcggcggca
    24721 acatcggcta ccgtctcgcc aagcagctcg aacacgcata caacgtcaaa atcatcgaat
    24781 gccggccgcg ccgtgccgaa tggatagccg aaaacctcga caacaccctc gtcctgcaag
    24841 gttcggcaac cgacgaaacc ctgctcgaca acgaatacat cgacgaaatc gacgtattct
    24901 gcgccctgac caacgacgac gaaagcaaca ttatgtccgc ccttttggcg aaaaacctcg
    24961 gcgcgaagcg cgtcatcggc atcgtcaacc gctcaagcta cgtcgatttg ctcgaaggca
    25021 acaaaatcga catcgtcgtc tccccccacc tcatcaccat cggctcgata ctcgcccaca
    25081 tccggcgcgg cgacatcgtt gccgtccacc ccatccggcg cggcacggcg gaagccatcg
    25141 aagtcgtcgc acacggcgac aaaaaaactt ccgccatcat cggcaggcgc atcagcggca
    25201 tcaaatggcc cgaaggctgc cacattgccg ccgtcgtccg cgccgaaacc ggcgaaacca
    25261 ttatgggaca ccataccgaa accgtcatcc aagacggcga ccacatcatc tttttcgtct
    25321 cgcgccggcg catcctgaac gaactggaaa aactcatcca ggtcaaaatg ggctttttcg
    25381 gataaaccgc cccattccgg acacattgcc gccaagcggt atggaagcgg aaataatggt
    25441 aggtgggctt cagacggcat ccgccctccc cgtccttccc gcgtaagcgg gaatccagac
    25501 cttgggatag cggcaatatt caaaggttat ctgaaaattt agaggttcta gattcccgtt
    25561 tccacgggaa ggacgaagag ttgcgggaat ctagaccatt ggaaagcggc aatattcaaa
    25621 ggttagctga agctttagag gttctagatt cccgtttcca cgggaatgac gaaaggtggc
    25681 gggaatccaa accattgggc aacagcaata ttcaaagatt atctgaaagt ttgaggttct
    25741 agattcccgc tttcgcggga atgacgaaaa gtggcgggaa tgacgaagtt tcagacggca
    25801 tcgcccgcct gttttgatat ggcggcaccc cccccgacaa aaaaaacaat ccggaacgca
    25861 tctgaccgtt ccggcttgtt ttcaggcgaa tccgccgcat cagaacatac tgcgcacgcc
    25921 catattgacc tgccaagtct ggcgcatcgt gtgcatcgaa gacctttgcg cctcaaaata
    25981 aagctgcctt ccgttgtcgg cattaccacg caaaaaaaat gaattgcttg atattccaat
    26041 gttttttata tgtttttata ttgtgatgcg atcagacaaa cgcccccctg acatttgttt
    26101 agacggcatc gtattgctaa atttctataa gtatgtataa tgtccgtttc cacgcgccca
    26161 tcgtctagag gcctaggaca ctgccctttc acggcggcaa ccggggttcg aatccccgtg
    26221 ggcgtgccaa ttcaaaaacc tgcttgtttc aagcaggttt ttttattatg aatcgtcatt
    26281 cccgcac
//
LOCUS       DAXHPG010000009        25169 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXHPG010000009 DAXHPG010000000
VERSION     DAXHPG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.009"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..808
                     /locus_tag="ACFM6F_000270"
     CDS             <1..808
                     /locus_tag="ACFM6F_000270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6163760.1"
                     /translation="RYRTPNIKYIYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINC
                     QVLLDFLSKDDIDMPDVVFLNAVKYDKGSVSHFGEDYQPEKILKQSKVEVLKGLCRFR
                     KFPGSACNKIIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQG
                     RKDSKTGTVSEKSIKSLLYILEKNAEMEFDRDISSYLYSFLSYEYLVLLFIMTSKNIE
                     CDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            849..1379
                     /locus_tag="ACFM6F_000271"
     CDS             849..1379
                     /locus_tag="ACFM6F_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC6163761.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1413..1529)
                     /locus_tag="ACFM6F_000272"
     CDS             complement(1413..1529)
                     /locus_tag="ACFM6F_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HFC6163762.1"
                     /translation="MPSEPEKRLSDGIFACNSNADGQNQGASDTVIGCVPSA"
     gene            complement(1744..3339)
                     /locus_tag="ACFM6F_000273"
     CDS             complement(1744..3339)
                     /locus_tag="ACFM6F_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC6163763.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRNTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVKPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3446..3841)
                     /gene="hisI"
                     /locus_tag="ACFM6F_000274"
     CDS             complement(3446..3841)
                     /gene="hisI"
                     /locus_tag="ACFM6F_000274"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC6163764.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGGAWETADAVLKDEKEIYGSTH"
     gene            complement(3872..4639)
                     /gene="hisF"
                     /locus_tag="ACFM6F_000275"
     CDS             complement(3872..4639)
                     /gene="hisF"
                     /locus_tag="ACFM6F_000275"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC6163765.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4652..5389)
                     /gene="hisA"
                     /locus_tag="ACFM6F_000276"
     CDS             complement(4652..5389)
                     /gene="hisA"
                     /locus_tag="ACFM6F_000276"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC6163766.1"
                     /translation="MLLIPAIDLKEGRCVRLRQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQTVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5422..6060)
                     /gene="hisH"
                     /locus_tag="ACFM6F_000277"
     CDS             complement(5422..6060)
                     /gene="hisH"
                     /locus_tag="ACFM6F_000277"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC6163767.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVWTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCIAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDRQGCRLKVPHMGWNTVRQTQNHPLFKDIPQNTRFYFVHSYY
                     FDPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(6191..7693)
                     /gene="pta"
                     /locus_tag="ACFM6F_000278"
     CDS             complement(6191..7693)
                     /gene="pta"
                     /locus_tag="ACFM6F_000278"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC6163768.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7878..8936)
                     /gene="fbpC"
                     /locus_tag="ACFM6F_000279"
     CDS             complement(7878..8936)
                     /gene="fbpC"
                     /locus_tag="ACFM6F_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC6163769.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8957..10474)
                     /gene="fbpB"
                     /locus_tag="ACFM6F_000280"
     CDS             complement(8957..10474)
                     /gene="fbpB"
                     /locus_tag="ACFM6F_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC6163770.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10542..11537)
                     /gene="fbpA"
                     /locus_tag="ACFM6F_000281"
     CDS             complement(10542..11537)
                     /gene="fbpA"
                     /locus_tag="ACFM6F_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC6163771.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11788..12258)
                     /locus_tag="ACFM6F_000282"
     CDS             complement(11788..12258)
                     /locus_tag="ACFM6F_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HFC6163772.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAIHSAMLIHRAADALRKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(12315..13691)
                     /gene="argH"
                     /locus_tag="ACFM6F_000283"
     CDS             complement(12315..13691)
                     /gene="argH"
                     /locus_tag="ACFM6F_000283"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC6163773.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(13710..14579)
                     /gene="galU"
                     /locus_tag="ACFM6F_000284"
     CDS             complement(13710..14579)
                     /gene="galU"
                     /locus_tag="ACFM6F_000284"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC6163774.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLVQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYERSGNSILGV
                     ETVEPSQTGSYGIVETEHLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14607..15206)
                     /gene="rdgB"
                     /locus_tag="ACFM6F_000285"
     CDS             complement(14607..15206)
                     /gene="rdgB"
                     /locus_tag="ACFM6F_000285"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC6163775.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSTFGIPECP
                     EPYSTFVENALAKARHAAKYSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALADLIHKLAL"
     gene            complement(15199..15432)
                     /locus_tag="ACFM6F_000286"
     CDS             complement(15199..15432)
                     /locus_tag="ACFM6F_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC6163776.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAQSAAKENRYV"
     gene            complement(15540..16073)
                     /locus_tag="ACFM6F_000287"
     CDS             complement(15540..16073)
                     /locus_tag="ACFM6F_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC6163777.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(16175..16633)
                     /gene="nudB"
                     /locus_tag="ACFM6F_000288"
     CDS             complement(16175..16633)
                     /gene="nudB"
                     /locus_tag="ACFM6F_000288"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC6163778.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16844..16920
                     /locus_tag="ACFM6F_000289"
     tRNA            16844..16920
                     /locus_tag="ACFM6F_000289"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16878..16880,aa:Pro,seq:ggg)
     gene            17078..18904
                     /gene="mafB"
                     /locus_tag="ACFM6F_000290"
     CDS             17078..18904
                     /gene="mafB"
                     /locus_tag="ACFM6F_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC6163779.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLFIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSNNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            18907..19371
                     /locus_tag="ACFM6F_000291"
     CDS             18907..19371
                     /locus_tag="ACFM6F_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163780.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            19596..19742
                     /locus_tag="ACFM6F_000292"
     CDS             19596..19742
                     /locus_tag="ACFM6F_000292"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163781.1"
                     /translation="MWYEANIGLINTMSRSNQAGTRLLYSNYGLLYITTDHYISATRF
                     VAWK"
     gene            19754..20041
                     /locus_tag="ACFM6F_000293"
     CDS             19754..20041
                     /locus_tag="ACFM6F_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFC6163782.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            20555..21793
                     /locus_tag="ACFM6F_000294"
     CDS             20555..21793
                     /locus_tag="ACFM6F_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HFC6163783.1"
                     /translation="MIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEALKKDKQ
                     TARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKESLYYLN
                     KHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGSCAETFG
                     LGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEYVQFSID
                     LFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAH
                     AQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVE
                     IKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSK
                     SGIKIEGFTEPNRTAYPIYE"
     gene            21824..22243
                     /locus_tag="ACFM6F_000295"
     CDS             21824..22243
                     /locus_tag="ACFM6F_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163784.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            22280..23254
                     /gene="mafB"
                     /locus_tag="ACFM6F_000296"
                     /pseudo
     CDS             22280..23254
                     /gene="mafB"
                     /locus_tag="ACFM6F_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            23265..23726
                     /locus_tag="ACFM6F_000297"
     CDS             23265..23726
                     /locus_tag="ACFM6F_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163785.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            23713..24000
                     /locus_tag="ACFM6F_000298"
                     /pseudo
     CDS             23713..24000
                     /locus_tag="ACFM6F_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
     gene            24027..24446
                     /gene="mafI"
                     /locus_tag="ACFM6F_000299"
     CDS             24027..24446
                     /gene="mafI"
                     /locus_tag="ACFM6F_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MafI"
                     /protein_id="HFC6163786.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            24536..24607
                     /locus_tag="ACFM6F_000300"
                     /pseudo
     CDS             24536..24607
                     /locus_tag="ACFM6F_000300"
                     /inference="COORDINATES: protein motif:HMM:NF026862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            24604..24846
                     /gene="imm31"
                     /locus_tag="ACFM6F_000301"
     CDS             24604..24846
                     /gene="imm31"
                     /locus_tag="ACFM6F_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC6163787.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            24924..25169
                     /locus_tag="ACFM6F_000302"
     CDS             24924..25169
                     /locus_tag="ACFM6F_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6163788.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
BASE COUNT         6234 a         5574 c         7040 g         6321 t
ORIGIN      
        1 cagataccga acaccaaaca tcaaatatat atatcaggat aaccaaggga cggcagaagc
       61 taggaatacc ggaataaaaa attcaaacgg aaaatatata gtctttattg attcggatga
      121 ttttattaat tgtcaggtct tgctggattt tcttagtaaa gatgatattg atatgccgga
      181 tgtggttttt ttaaatgcgg ttaaatatga taagggaagt gtttcacatt ttggcgaaga
      241 ttatcagcct gaaaaaatac tcaagcaatc caaagtcgaa gttttgaaag gattatgccg
      301 atttagaaaa tttccgggtt cggcgtgtaa taagattata aaaagggaat tgattatcaa
      361 agaaaaactg ttttttgaaa aagaggttta tgccgaagat atcgaatggt caatgagatt
      421 atttaatgcg gcaacaactt tttcttattt ggacggttgt tattactatt atcggcaggg
      481 aagaaaagat tctaaaacag gaactgtttc ggaaaaaagt ataaagtcat tattatatat
      541 tttggagaaa aatgcggaaa tggaatttga tagggatata tcaagttatc tttattcttt
      601 tctttcctac gaatatctcg ttttgctttt tatcatgacg agtaaaaata tagagtgtga
      661 tgctgatata aaaagaaggg cgtatcattt aaggtttatg ctgttaaagt ccaataaatt
      721 gatatataag ctgatattcc cgataatcac attattcggg gtcgatatta caggcaggat
      781 tttaaaagca atcaggggga atatttaata aatcctttaa cactatacat cttaccgaag
      841 gaggaaaaat gaacgcaatc cgaactttcc aaaaccgcac gcccgaaatc cacgaaagct
      901 gtatgataga cgaagcgtgc gtcgtcatcg gcgaagtgtc gcttgccgaa gacgtttccg
      961 tgtggccgtg cgccgtgttg cgcggcgatg tgaacagcat caccgtcggc gcgcgcagca
     1021 atatacagga cggcagcgtc ctgcacgttt cccacaaaac cgccgccaaa cccgaagggt
     1081 cgccgctggt tatcggcgaa gacgttaccg tggggcacaa agtgatgctg cacggctgcc
     1141 gtatcggcaa ccgcgtcctg gtcggcatgg ggacgacggt tctggacgat gccgtgattg
     1201 aggacgaagt gatgatcggc gcgggcagcc tcgtgcctcc gcgcaaacgt ttggagggcg
     1261 gctatcttta tgtcggttcg ccggtcagac aagtgcgcgt gttgaccgat gaggaaaaag
     1321 cctttttgaa gtattccgcc gcgcattatg tgaagctgtc gaaacagtac gggatgtgaa
     1381 atcacatcgg cgttcttgcg tcagccccaa attcatgcgg atgggacgca tccgataacg
     1441 gtatccgatg cgccttgatt ttgaccgtct gcgtttgagt tgcaggcaaa aatgccgtct
     1501 gaaagccttt tttcgggttc agacggcatt ttatcgccta aagtttgacc gaatgttcgc
     1561 gcgtttcgtg gaacacgatg tccggccagc gttcttgcgt cagccctaaa ttcatgagga
     1621 cgggatgccc gataacggta tccgatgcgt cttgattttg atcggtgcat ttgagttgca
     1681 ggcaaaaatg ccgtctgaaa gccttttttc gggttcagac ggcattttat cgccgattgc
     1741 tttttacagt ttaaccgaat gttcgcgcgt ttcgtggaac acgatgtccg gccaacgttc
     1801 ttgcgtcaac cccaaattca cgcggttggg ggcgaggtag gcgaggttgc cgcccgcgtc
     1861 gatggcgagg ttgcccgcgt tggctttttc aaactccgcc agttttttct tgtcgtcgca
     1921 cgatacccag cgcgccgacc agatggatgc gctgtcgaac acggcttcta cgccgtattc
     1981 gttggccagc cgcgaggtaa cgacttcaaa ctgcaacacg ccgaccgcgc ccaaaatcaa
     2041 atccgcgccg ctcatcggtt tgaatacctg taccgcgcct tcttcgccaa gctgttgcaa
     2101 acctttttgc agttgcttga ttttcagcgg gtttttgatg cggacgctgc ggaacagttc
     2161 gggcgcaaag aatgggatgc cggtaaacgc cagttgttcg ccttcggaga agctgtcgcc
     2221 gatttggatg ttgccgtggt tcgggatgcc gatgatgtcg ccggcgtagg cttcttccac
     2281 cagctcgcgg tcgtgcgaca taaaggttac cacgctggag gcagcgattt cgcggttgat
     2341 gcgcaggtgt ttcatcttca taccgcgctc gaatttgccg gagcagacgc gcaagaaggc
     2401 gatgcggtcg cggtgtttcg gatccatatt ggcttggatt ttaaagataa atccggagaa
     2461 tttcggctcg tccggcttga ccatacgtac ggtcgcgtcg cgcggtttcg gcgcgggcgc
     2521 ccagtcaatc aatgaattga ggatttcctg aataccgaag ttgttaatcg cagagccgaa
     2581 gaatacgggc gtgagttcgc cggcgaggaa ttcgtcgaga ttaaactcgt tggaagccgc
     2641 ctgcaccaat tcgatttcgt cgcgcaactg ctggatttcc aatggaaagc gttgttccaa
     2701 ttcgggattg tcgatgcctt tgatgatgtc gaactcgtgc ggcagacgtt cgccaccggc
     2761 ttcaaagaga tagatttcgt cgttcaggat gtggtacacg cctttgaagt ttttgcccat
     2821 gccgatcggc caggttacgg gcgcgcagcg gatttgcagg atgttttcca cttcgtccag
     2881 caactccaaa gaatcgcgca cttcgcggtc gtatttgttc atgaacgtga caatcggcgt
     2941 attgcgcagg cggcagacgt tcaagagttt gatggtttgc gcttccacac cttttgccgc
     3001 gtcgatgacc atcaaggcgc tgtccacggc ggtcagaacg cggtaggtgt cttcggagaa
     3061 gtcttggtgt cccggcgtgt ccaagaggtt gacggtgtgg tctttgtagt cgaactgcat
     3121 cacgcttgat gccacggaaa tgccgcgctg cttctcgatg tccatccagt cggaggtggc
     3181 gaatttgccg gtttttttgc cttttaccgt acccgcgctc tgaatcgcgc ccgaaaacag
     3241 caacagcttt tcggtcagcg tggttttacc cgcgtcgggg tgggagatga tggcaaacgt
     3301 gcggcggcgg cgcacttggt cgaggatttc ttgggacatg gttttctttg caaaaaggtt
     3361 caggccgctt ttcagacggc ccggacagtg tttgagacgg cgaaattgta caaaaaaatg
     3421 cctgataatt caatgttgga ggcggtcagt gcgtgctgcc gtaaatctct ttttcgtctt
     3481 tcaggacggc atcggcggtt tcccacgcgc cgccgcgcca gactttgtaa aagcagcttt
     3541 ctcgcccggt gtggcaggcg atgccgccgt tttgggcgat gagcatcaca atggcgtcgc
     3601 cgtcgcagtc gaggcgcagt tcgcggactt tttgcgtgtg tcccgactct tcgcccttca
     3661 tccattgttt ttggcgcgaa cggctgtaat agtgggcaaa gccggtttcg acggtttttt
     3721 gcagggcttc ggcgttcatc cacgctacca ttaaaacacg tttggtttcg gcatcttggg
     3781 cgatggcgca aaccaaacct ttttcgtcaa atttgacggc ttcaagcagg tttttatcca
     3841 tatttccttt cagacggcat agtcggggcg gtcagaggcg cacttcgatg ccggcttcgc
     3901 gcatagcgcg tttggcttcg cggatggcga tttccccgaa atggaaaacg ccggcggcaa
     3961 gtacggcatc ggctttgcct tcggttatgc cttcaatcag gtgccggaca ttgccgaccc
     4021 cgccggaggc gatgacgggg atgtcgacgg cttcggcaac ggcgcgggtc agcggcaggt
     4081 tgaaaccctg tttcgtaccg tccctgtcca taccggtaag caggatttcg cccgcgccgc
     4141 gtttttgcat ttcgatcgcc cattccaccg catccaaacc ggtcggattg cgcccgccgt
     4201 gggtaaagat ttcccagcgt gtgttttcgg ggttgacggc tttggcatcg acggcggcga
     4261 cgatggcttg cgaaccgaaa aatccggcgg cttcgtcaat caggtcggga cgggtaacgg
     4321 cggcggtgtt gatgctgact ttgtccgcgc cggcattgag caggcggcgg atgtcggcaa
     4381 cggtgcgtac gccgccgccg acggtcagcg ggatgaagac ttgtccggca acttcttcga
     4441 tgatgtgcag gatggtatcc cggttgtcgg atgaggcggt gatgtcgagg aaggtcaatt
     4501 cgtccgcgcc ttcgccgttg tagcgtttgg cggcttcgac ggggtcgccc gcgtcgcgca
     4561 aaccgatgaa gttcacgcct ttgacgacgc gcccgtcttt tacgtcgaga caggggatga
     4621 tgcgttttgc cagtgccata atcggatgcc tttagtcgag ggaatctgcc agttgctgcg
     4681 cttgggcaaa atcgatgctg ccctcgtaaa tcgcgcggcc ggtaatcgcg cctgctacgc
     4741 catgtttttc ggcggcacac agggcgcgga tgtcgtccaa gccggtcagt ccgccggagg
     4801 cgatgacggg aatgcggacg gtttgggcga gtttgaccgt cgcgtcgatg ttcacgccgc
     4861 tcatcatacc gtcgcgcccg atgtcggtgt agatgatgct gttgacgccg tcgtcttcaa
     4921 agcgttttgc caaatcaatt acatgatgcc cggttacggt tgcccagccg tcgatggcgg
     4981 ccataccgtc tttggcatcc agcccgacaa taatcctgcc ggggaaggct ttgcacgcct
     5041 cgcgcacgaa gtcggggttt ttgaccgccg ccgtgccgat aatcacgtcg ttcagaccca
     5101 aatccaaata ttgtccgatg gttttcaaat cgcgtatgcc gccgccgagc tgtacgggga
     5161 tgtctttggc gacggcggca aggatgtctt tgatggcggg caggttttgc ggaacgccgg
     5221 caaacgcgcc gttcaaatct accagatgca ggcggcgcgc gccttgtttg aaccagtgca
     5281 gtgcggtttc ggcgggcgaa tcggaaaaga cggtcgcctc ttccatcagc ccttgtctca
     5341 ggcggacgca gcgtccttct ttcaaatcga tggcgggtat cagcagcata atttttctcc
     5401 ttgtgcgggg ccgtgtccgg cttaccagtt taaaaagttt ttcaacatcg tcagcccggc
     5461 atcgtggctt ttttcggtgt gaaattgcgt ggcgaatacg ttgtctttgc cgacgatgca
     5521 ggcaaacggg gacgggtagt cgctttcgcc caatatggtt tcgggatttt cggggtcgaa
     5581 atagtagctg tggacgaagt aaaaacgcgt gttttgggga atatctttaa acagcgggtg
     5641 gttttgggtt tggcgcacgg tgttccagcc catatgcggg actttcagac ggcatccctg
     5701 ccggtcgcgg aggtcgcgct caaagcgtct gactttgccg ccgaaccagc ccaagccgtc
     5761 ggtgtttcct tcttcactgt ggtcgaataa aagttgcgcg ccgacgcaga ttccgaaaaa
     5821 gggtttgttt tttaaggcat ctttgactgc ctcgtccaag ccgccgcgcg tcagtgccgc
     5881 aatacagtcg ggcatcgcgc cctgcccggg aaaaatgact ttgtcggcgc gggacacgcg
     5941 ttcggggtcg ccgcttaaaa agatttcggt attttttccg gcaagctgcc ccgccgtcca
     6001 gacggatttc aatacggaat gcaggttgcc cataccgtaa tcgataatgg cgatttgcat
     6061 ggcttcctcc tctttttttg caatatggct gcgattttaa caaacaaatg tgccgtgctg
     6121 ataaaaatgc cgtctgaaaa cgggagtctg tcttcagacg gcatagggtt taaacccgga
     6181 aagccgtttg tcagccttcc atttgttttg cctgaacggc agttagggcg atggtgaaca
     6241 cgatgtcttc caccagcgcg ccgcgcgaca ggtcgttgac cggtttgcgc aggccttgca
     6301 gcagcgggcc gacgcttaag acgttggcac tgcgttggac ggctttatag gtgcagttgc
     6361 cggtgttcag gtcggggaag accaaaacgg ttgcctgtcc tgccaccggg ctgcccggag
     6421 ctttggattt gcccacgccc ggcacggttg ccgcatcata ttgcagcggg ccgtcgatgg
     6481 cgaggtcggg gcgtttttcc cgggcaagtt tggttgcttc gatgacggta tcgacatcgg
     6541 ggccgctgcc ggagttgacg gtggagtagg aaatcatcgc cactttcggg tcgatgccga
     6601 aggcttttgc ggaatcggca gactggatgg cgatgtcggc aagctgttgc gcggtcgggt
     6661 tcggattaac cgcgcagtcg ccgaagacga ggacttggtt gggcagcagc ataaagaata
     6721 cgctggacac gaggcttgcg cccggtgcgg ttttaatcag ttgcaaagcg gggcggatgg
     6781 tgttggcggt ggtgtgaacc gcaccggata ccaaaccgtc cacatcattt tgcgccatca
     6841 tcatcgtacc gagtaccacg gtgtcttgca gttgcttgcg cgcgtcttcg ggtgtcaggc
     6901 ctttggattt gcgcagttcg cacatcggtc cgacgtattg ttcgaccaat gaggcgggat
     6961 cgatgatttc caaagagtcg ggcaggctga tgccgcgttc tttggcaacg gcttcgactt
     7021 cttcgcgttt ggcaagcagg acgcagcggg caatgccttt ttcgtggcag atggcggcgg
     7081 cttggacggt gcggggttct gcgccctcag gcaggacgat gcgtttgtcg gcttggcggg
     7141 cgaagtcgat caggttgtag cggaattgcg ccggcgacag gcgttttgct tcgcggcctg
     7201 ccaatacgga tacgtctttc agcgcgtcgc tcgaaccgaa gaaggtcagg ccggtttttt
     7261 cggctgccgc ttcggcaacg gaggctgccg cgccgtccac gacaaaacct tccaatacgc
     7321 ccggcgcggc ggcgaagaac tgtttggcaa ggttcagccg atttgccagt tcgtcggcat
     7381 cggcgttgtc ggaacggacg gcgaagacgg ctgccgcgtc aagggacaat gccagttcga
     7441 cgtttttgcc tgcgaggtag attttgtcgg catcgggcgc gatgccttcg atgacgaggt
     7501 tggcggcatc gagtgcggca actttgccga ccagtgcgtc gaaccagtcg tcgcttttgc
     7561 cttgcgcgag cagggtttcg gcggttgcgt caacggcttg gaaaatttgt gcgtccagtg
     7621 cttttgcaaa ggcttgtgcg gcggcggagg cgtccagtcc ggcagatacg ggtacgatga
     7681 gtacttttgc catgatatat cctttcgtat gctgcggtgt gcggcatatg tggttggaag
     7741 gggcggcata taggcagaaa cggctgcctg cgtgccgtgc gtgccgtgtt tggcttgagg
     7801 cgcgcaggtt gaatatagca aacaaattct gtttccaaca agataaatat ccgcaggctt
     7861 gtggatgctg ccgcctttca gagggtattt ccggggaaga acagggcggg accgtccaaa
     7921 tggaggacgg cggaaatgcc gtctgacagg gtgggggcgg aagggaggtt gagcgtgagg
     7981 acggtttgtc cgacccggag gctgatttcg gtatgccgcg ctttgggcgt ggttttgaga
     8041 accacggcgt gaatggaggc ggtgggtgcg gaatgggggt gaaggctgaa ctgttccgga
     8101 cggatgagca gtgtgccgcg tgtgcctgcg ggtgcgccgc tttgaacggg caggcggccc
     8161 aatccgcaat cggcggtgcc gtcggcgttg agcgcggcgg ggaacacgat gccttcgccg
     8221 ataaacaggg cggcatcaag gtcggcaggt tgtcggtaca attcgtgagg gcttgcagtt
     8281 tggaggatgc gcccctgttt catcacggca atccggtcgg cgtattgcag ggcttcttcg
     8341 cggtcgtggc tgacgaaaac ggcagatttg ccgttggcgc gcagggcggc aatcatgtct
     8401 tcgcgaatct ggcggcgcaa ctgttcgtcc agcgcgctga agggttcgtc caacaaaatc
     8461 agttcgggat cgggtgcgag ggcgcgggcg agggcgacgc gctgttgctg tccgcccgaa
     8521 agttcgtgcg gatagcgtcc ggcaagttcg gaaatgccgg tcaattccaa catagcttcg
     8581 atgcgctgcc gctcttgcgc cgtcttgcct ttgccgttgc ccagcccgta ggcggtgttg
     8641 cggtaaacgg tcaggtgggg gaacagcaca ccttcctgta cgacataacc caaacggcgt
     8701 tcgcggacgg ggaggttggt atttttcgag aagatggttc tgccggaaag cgaaatttcg
     8761 ccaaaatcgg gttgttcaaa accggcaagg cagcgtaaaa gggtggtttt gccgcagccg
     8821 gacgcgccga cgataaagag gatttcgccc gggtcgaggc tgagcgaaat gtcgtttaaa
     8881 actggggtgt tttgaaaact tttggacagg tgtccgatgt gcagggcggc ggtcatggcg
     8941 gtacttcctc aagctgttat ttgaaggcgt atttcttcag caggaatacg gggatgccgg
     9001 aaaataatac cagcatcagc gcgtaagggg tggcggcggc gtattgtgcg tccgatgtgt
     9061 attcccaaac ggcggtggag agtgtgtgga catcgtcggt ggtcagcagc agggtggcgg
     9121 tcagctcttt catcagtttg aggaagacga gtgcgaatgc ggcggtaatg ccgggcagga
     9181 tggacggcag taccaacgtc ctgaaaataa agaagtgtcc gcgccccaat gttgcgccga
     9241 cctgttccat cccttttggg agttgttcca aggaagtcct cagggtggtt tgcgccatcg
     9301 gcaggtaaag catgaaatag gcaaggatga cgacgataaa ggtttggtaa acggcagggg
     9361 tgtagttgat gctgaaataa accaaggata gggcgataac caaaccgggg acggcgtgca
     9421 gtaaaaacgg cagcctgtct atccaaacgg ttaaaaaatt gcgatagcga accgatgccc
     9481 aaacaagggg caaggcacat aatatagtca aaatcgcacc taaagccgat acgcttaagg
     9541 aacggataaa ggcatcaaat acggatacga gcgcgaatgt gccggaagtg ccgaccatca
     9601 tccaatgtat caatacgcca aaggggataa taatgcccaa agtcaacaag ctgcttaaaa
     9661 aaacaatcgc gccaatctga ccgggcagtt tgagggtttt gacgggataa ggacgggcaa
     9721 cgcctttgcc gctgtggtaa atcttggctt tgccgcgaaa tatgctttct ccaaatacga
     9781 cgatgccgca caccgccatt aaaacagcgg aaagcagggc ggcggtattg ttgttgtagg
     9841 acatttcgta ttcttggaaa atggcggtgg taaaagtggg gtagttcaaa atggataccg
     9901 cgccaaattc gaccaacata tgcagggcaa tcagtaacac gctgctgccg atggcgggtt
     9961 tgagctgggg gaggatggcg gaaaaaaagg tttgcaggcg gcttttgccc aaggacaggc
    10021 tgacttcttc gtaagacagg ctgatgcgtt tgagtgccgc ctcgacgggc aggtaggcga
    10081 gcgggaacga ggacaggctc ataatcatca ctgtccccca aaagccttcg acacggaagg
    10141 tcaggctgat ccaggtgaaa cagctgacaa atgcggggat gcacaaaggc agggtgattg
    10201 ccgtctgaaa aaaggttttg ccgaagaagc ggtaacgttg gaacaaaagg gcgcaggcaa
    10261 tgcccaaaac aatggaaatc agggtaacgc ccgccatcat cgtcaaggtg ttggagagca
    10321 aatcccacat acgcgggcgg aacaacagtt cgacggcgcg gttgatgccg acctgccacg
    10381 aacgcatagc gacatataaa aaaggcaggg taagcggtag ggcaatcagt aggatgaggc
    10441 cggtaagcca aatgggtatt tttttaggag acatagtgtt ttttatcggc aaaacgggcg
    10501 gacagtataa atgtccaccc gtttgacaat ccgaaaacgg cttatttcat accggcttgc
    10561 tcaagcagcc gggtggcgtg ttctttttcg gaaacagtgg tggcggacac ttggggtgct
    10621 tccaacttgg cgatgggttc caaattgaag gtggatacca cgtgcggatt caaaggatac
    10681 tcggcacgga cggcggtcag ggcgcgctgt ccttccttgc tggcgaggaa ggcgacgaat
    10741 tttttcgcct catccttgtt ttgggaggat tttaacacgg ctgcgccgga ataggtaacg
    10801 agtgcgccgg gatctctgtg gcggacgaaa ttcaggcggg tgtggacatt ttgtacgcct
    10861 ttttcacgcg caaaagcgtg ccagtagtag ttgttgatga gggcggcatc gatttcgccg
    10921 ttttcaaccg cttgaagggc gacggagttt ttagcgtaag gcttgccgta ttctttcaga
    10981 cctttgagcc atttcaatgc ggccgcttcg cctttcagtt tgacgatggc gacaacctgt
    11041 tccaagaacg cgccggaagt gggggcgtaa ccgatgcggt ttttccattt cggcgtggcg
    11101 taattcagga cggatttttc caaatctttt tcagacagtt tgcgggtgtc gtaaacgacg
    11161 acgcgcgaac gtccgctcag tgccacccag tcttttttgg cggcaaccgg cacgcccttg
    11221 ccgcgtgttt cgttgatggt ggaggcgggc aggggctcta ggaggttggc ggcggaaagg
    11281 gtggcgagtg ccgggatttg ttcggaatag aatacgtcgg cggggcttcg gctgccttct
    11341 tctttgattt gaccggcaag ctggtcgcct ttggcgctgt tgagtttgac tttgatgccg
    11401 gtagcccggg taaaggcatc tgcaacggct tgtgctgctt ctttgtgttg gccgttgtac
    11461 acggtaatgt ctgccagcgc gggggttgcg gcggtcaggg ctgcggcaag cagtgcgtat
    11521 cggatagatg ttttcatatc gattttctcc taatgaatga gagtgtacac cttgttaaaa
    11581 cataacggtg tgtagtgtat tccttcttct ttataaatgc aaataattat tttttaaatt
    11641 tgttattatc cgatccggtt atggtttgtt ctgacttgta ttttttccgt gcatcgcgcc
    11701 cgtaaggcgg aagcggcggg caatgcgtgg cggaatgtgg gtaaaggcgg cattttgatt
    11761 tgtcggaatg cttgagaacc cctctcttta aaacagcgtc tgaacagggt tgccggaata
    11821 gtattgccat cccggcagat acagtttgtc ggggtcttgc caatattgtt catccagact
    11881 gttcggcagt gaggcggttt tgttgtcgag atgttttgtt atatcccaca tttctttcag
    11941 gtttttacct tccgattgga ggtggcggat ttcttttctc aatgcgtctg ccgcccgatg
    12001 gatcagcatt gcgctgtgta tcgcgccgat ttttttcaga acggaacaag tcttttcggc
    12061 ggcgggaaaa ccccaattgc agacaaattg cagtatgccg ccgttaccga tatcggcttc
    12121 tgtccgccaa atcaaaacca gctcctgttc ccgcccgtcc atgctttcga gtttgccgtc
    12181 atgctgttca aaaagtttgt ccaccgcctg ccacatcatc tgttcgaagc ggtcggcttg
    12241 ggtatccgta tcggtcatcg tgttcttgcc ttttaaaaat gccgtctgaa catttcttca
    12301 gacggcatcg ggggttaagc caacatttcc cgccagcgtt tgacttggaa acggacttgt
    12361 tcaggcgcgg tgccgcccaa gtggttgcgc gcgtttaagc tgccttcggg tgtcagcacg
    12421 ccgtaaacgt cgtcggcaat caaatcgctg aaaccttgta agacttcgag cggcagttcg
    12481 ctcaaatcga cgccagcttc atcggcgtgg cgcacggctt gggcgacgac ttcgtgggca
    12541 tcgcggaagg gcataccttt tttaaccaga taatccgcca agtcggtggc ggtggcaaaa
    12601 ccctgcatca cggcggcgcg catattgtcg ggtttgacgg ttacgccgcg catcatatcg
    12661 gcgtaaatcc gcagcgtgtc gataagcgtg tcggcggtgt caaacaaagg ttctttgtct
    12721 tcctgattgt ctttgttgta cgccaagggc tgcgatttca tcagggtaat caagccgata
    12781 aggtgtccga tgactcggcc ggatttgccg cgcacgagtt cgggcacgtc ggggtttttc
    12841 ttctgcggca tgatggacga acctgtgcag aaacggtcgg cgatgtcgat aaagccgaaa
    12901 cgcggactca tccacaaaat caattcttca gacaggcggc tcaggtgaac cataaccagc
    12961 gaggcggcgg cggtgaactc gacggcaaaa tcgcggtcgg atacggcatc gagcgagttt
    13021 tggcagattt gttcaaagcc caatagctcg gcggtaattt cgcgctgaat cgggtaggtc
    13081 gtcccggcaa gggcggctgc gccgagcggc atacggttga cgcggcggcg gcagtccgcc
    13141 atccgttcgt tatcgcggcc gagcatttcg acgtaggcga gcatatggtg tccgaagctg
    13201 acgggctggg cgacttgcag gtgggtaaag ccgggcataa cggtttcggc gttttgttcc
    13261 gccaaatcca gcaatgccgt ctgaaggttt tgaatcaggc tttggataac ggtaatctgg
    13321 tcacgcagcc acaggcggat gtcggtggcg acttggtcgt tgcggctgcg gccggtgtgc
    13381 aggcgtttgc ccgcgtcgcc gattttgtcg gtcaggcggc gttcgatgtt catgtggaca
    13441 tcttccaaat cggacgacca ttcgattttg ccgctgcgga tttcttcgag gatttccgcc
    13501 atgccccgac ggatgtccgc caaatcgcct tcgtccaaca cgccggtttc tttcaacatt
    13561 tgcgcgtgtg ccagcgagcc ttgaatgtcc cattcggcaa gccgtcggtc gaaaccgatg
    13621 gaggcggtgt attgtttgac gagttcggaa acgggttcgt tgaaacgtcc ggaccaggtt
    13681 ttgtcgtgca taaggattcc ttgatggggt tattcggtgc ggtatttttc caaaaggcgg
    13741 cggaagggtt cgccggtttc ggggtgtttc aggccgtagg cgacggtggc ttcgaggtag
    13801 cccagtttgc tgccgcagtc gtagcgcgtg ccttcaaagg ggtgcgccag gacaaattcg
    13861 tgatcgagca gcttggcgat gccgtctgta agctggattt cgttgcccgc gccgcgcggc
    13921 agtccggtca gtaagtcgaa aatgcgcggg gtaaggatgt agcgtccgac gacggcaagg
    13981 ttggagggtg cgtcttcggg cttgggtttt tcgacaatgc cggtaatgcg ttggaactgt
    14041 ttgagatgtt cggtttcgac gatgccgtat gagccggttt gcgacggttc gacagtttct
    14101 acgcccaaaa tgctgttgcc gctgcgttcg tacacttcga ccatttgttt gagcgcgcct
    14161 ttgggggcat caatcaggtc gtcggcaagg ataacggcaa agggttcgtc tccgatggcg
    14221 gcgcgggcgc acaagacggc gtgtcccaag cccagtgctt ccgcctgacg gatgtagagg
    14281 caggtaatgt tcggcggcag gatgttgcgg acgtgttcca acaatttgtc tttatggcgc
    14341 atttccaact cggtttcgag ttcgtatgcc ttgtcgaaat ggtcttcgat gctgcgtttg
    14401 ttgcgtccgg taacaaacac catttccgtg cagccggctt ccacggcttc ttctacggcg
    14461 tattggacca gcggcttgtc gacgatgggc agcatttctt tcgggcttgc cttggtggcg
    14521 ggcaggaagc gtgttcccat ccctgcgacg gggaaaacgg ctttccgtat gggtttcatt
    14581 ctttttcctt tgtattgttt tgatgtttaa agggcgagtt tgtgaattaa atcggcgagt
    14641 gcctgtgcgc ggtggctttc gcggtttttg acctccgaat ccaattcggc ggcggttttg
    14701 ccgtgtccgg gcagataaaa atacgggtcg taaccgaaac cgttttcacc ggcgggaacg
    14761 ccgttccatt gtccgtgcca tacgccttcg gcaataatcg gacgcgggtc gtctttatgg
    14821 cggacaaaaa ccaatacgca gacgtagcaa cagcttttgt cggctttgcc ggcaagttcg
    14881 gcggcaagtt tcaggttgtt ggcggtatcg gatttgggat tgctgccggc gtaacgtgcg
    14941 gaatggatgc ccggagcacc gtttaaggcg gcggcacaga tgccgctgtc gtcggcgagt
    15001 gcgggcagcc cgctgtattt ggcggcatgg cgtgcttttg ccagcgcatt ttcgacaaag
    15061 gtggaatagg gttcggggca ttcgggtatg ccgaatgtgg attgcggcaa tacggtgatg
    15121 ctgtaaggtt tgaataagtt gccgaattct tcgagcttgc cggcattgcc gcttgccaaa
    15181 acgatttttt ccggtttttc agacatagcg gttttccttt gcggcggatt gggcggcgcg
    15241 tagggatttg tgccgcaggt agagggcgag tgcgccgatt tgcccgaaca gcgcgccgaa
    15301 tgcgaaaaga aaggaggcga cggcgaaggc tttgctgccg acggtcagca gataagcacc
    15361 caagaggacg agcagcataa atgccagtgt gaagagggca acagacaaca gatagatttt
    15421 tcggcggttc atggcgttcg gtcggaaacg gtatgttcgg attatatccg attgggacgg
    15481 tattccctag agcttggaaa aatgccgtct gaagcggcgt tcagacggca tggcgggtgt
    15541 tatgcctgtt tgttccaacg ttcgatggat tctttaatga cttttttcgc ttcttccgca
    15601 tcgccccacg attcgacttt ggtcgtacct gcttttttca ggtctttgta atggttgaag
    15661 tggaactcga tttgtttgat gagctgttgc ggcaaatcgg acaaagtttt gtaggcgttg
    15721 ccgttgttgc ggtcgtcggc aggaacgcag acgattttgt cgtccacttc gccgtcgtca
    15781 acgaatttca tcacgccgat aacgcgcgct tccaagaata cgccggttgc caaaggttgt
    15841 tcggtaacga gcagcacgtc caattcgtcg ccgtcttcgt ccaaagtttg gggaatgaag
    15901 ccgtagttgg tcggtttggc gaagatggcg ggttcgacgc ggtcgagttg gaatgcggcg
    15961 agtttgcggt tccactcgat tttgtggttg ctgccggcgg ggatttcgtt gacgacgttg
    16021 atgatgccgc cgtccacatc gccgggggtc aggatttggt tgaagtctgc catttggttt
    16081 cctttgtttg ggaaagtttg aagtttgaaa gtatagcaca aacgtccggc tgaaaatgcg
    16141 cccgatgccg tctgaaaggg tgtacgggcg cgtgttaccg tttgcccaaa aacctgccca
    16201 gttccagaat cgcgcgcctg ttggacgggg aaaacacttt ttccgccgct tcttccaagc
    16261 cgaaccagcc gtaggagacg tgttcttcgg gttgcagggc gatgggcgta tcacgcggga
    16321 tttcggcaga gaagaggtgt tcgcggtttt caaacacgcc ttttggatag cggtgccgcc
    16381 agtggtggta gatttcgtaa accgtgctgt cgtgccagtc ttgaagctgc ccgtccgcca
    16441 gcaggatgcc ggtttcttcc caaacttcgc gccttgccgt ttgggcgacg gtttcgcccg
    16501 gttcgaggct gccggttacc gactgccaaa atccttccgg atgcgtgcgt tcgatgagca
    16561 ggatgccgcc gtccccgcta taaaggacga ccagtgcgga gacggggtat ttgagcggtt
    16621 ttgccatcgg catctttcgg cgggctgcgg taatgaaggg gcggattata gcaaacgccg
    16681 cacgttatgg cgtttatcct tttccgtatc cttttttctg cacggatggg acgcgccggt
    16741 gtttgccggt aaattttccg ttgtgtcaaa aagataaggg cggttgtgat tttaatgctt
    16801 gccaaagcgt cgggcggaaa ctataatccg aaacttatcg agtcggagtg tggcgcagtc
    16861 tggtagcgca cttgcatggg gtgcaagggg tcgaaggttc gaatcctttc actccgacca
    16921 aaaattccga aagccgcttt caaaagcggc ttttttgccg tccgtatgat tatgatgtag
    16981 agtacgcggc gacagacatt caaatgccgt ctgaaaaccg ttcagatggc atctctttat
    17041 cttagtttca ttccgtacca tcttaaggaa catcaaattg ggcatttccc gcaaaatatc
    17101 ccttattctg tccatactgg cagtgtgcct gccgatgcat gcacacgcct cagatttggc
    17161 aaacgattct tttatccggc aggttctcga ccgtcagcat ttcgaacccg acgggaaata
    17221 ccacctattc ggcagcaggg gggaacttgc cgagcgcagc ggtcatatcg gattgggaaa
    17281 catacaaagc catcagttgg gcaacctgtt catccagcag gcggccatta aaggaaatat
    17341 cggctacatt gtccgctttt ccgatcacgg gcacgaagtc cattccccct tcgacaacca
    17401 tgcctcacat tccgattctg atgaagccgg tagtcccgtt gacggattca gcctttaccg
    17461 catccattgg gacggatacg aacaccatcc cgccgacggc tacgacgggc cacagggcgg
    17521 cggctatccc gctcccaaag gcgcgaggga tatatacagc tacgacataa aaggcgttgc
    17581 ccaaaatatc cgcctcaacc tgaccgacaa ccgcagcacc ggacaacggc ttgccgaccg
    17641 tttccacaat gccggcgcta tgctgacgca aggagtaggc gacggattca aacgcgccac
    17701 ccgatacagc cccgagctgg acagatcggg caatgccgcc gaagccttca acggcactgc
    17761 agatatcgtc aaaaacatca tcggcgcggc aggagaaatt gtcggcgcag gcgatgccgt
    17821 gcagggtata agcgaaggct caaacattgc tgtcatgcac ggcttgggtc tgctttccac
    17881 cgaaaacaag atggcgcgca tcaacgattt ggccgatatg gcgcaactca aagactatgc
    17941 cgcagcagcc atccgcgatt gggcagtcca aaaccccaat gccgcacaag gcatagaagc
    18001 cgtcagcaat atctttacgg cagtcatccc cgtcaaaggg attggagctg ttcggggaaa
    18061 atacggcttg ggcggcatca cggcacatcc tgtcaagcgg tcgcagatgg gcgcgatcgc
    18121 attgccgaaa gggaaatccg ccgtcagcaa caattttgcc gatgcggcat acgccaaata
    18181 cccgtcccct taccattccc gaaatatccg ttcaaacttg gagcagcgtt acggcaaaga
    18241 aaacatcacc tcctcaaccg tgccgccgtc aaacggcaaa aatgtcaaac tggcagacca
    18301 acgccacccg aagacaggcg taccgtttga cggtaaaggg tttccgaatt ttgagaagca
    18361 cgtgaaatat gatacgaagc tcgatattca agaattatcg gggggcggta tacctaaggc
    18421 taagcctgtg tttgatgcga aaccgagatg ggaggttgat aggaagctta ataaattgac
    18481 aactcgtgag caggtggaga aaaatgttca ggaaataagg aacggtaata aaaacagtaa
    18541 ctttagccaa catgctcaac tagagaggga aattaataaa ctaaaatctg ccgatgaaat
    18601 taattttgca gatggaatgg gaaaatttac cgatagcatg aatgacaagg cttttagtag
    18661 gcttgtgaaa tcagttaaag agaatggctt cacaaatcca gttgtggagt acgttgaaat
    18721 aaatggaaaa gcatatatcg taagaggaaa taatagggtt tttgctgcag aataccttgg
    18781 caggatacat gaattaaaat ttaaaaaagt tgactttcct gttcctaata ctagttggaa
    18841 aaatcctact gatgtcttga atgaatcagg taatgttaag agacctcgtt ataggagtaa
    18901 ataaaaatgg caatttggaa ttatcggtac taccttatcc catcagctgc tatcagaaat
    18961 aaatttaatg caaataaaga tatcatactt gatgagtatc gatctaatgg ttttcagaat
    19021 tttaatgaga ataaaagttt tgaaaattac tttatcgata atgatgttat attattatca
    19081 ataataaatg aagcaaaaaa acagcttaaa ttgaaagaat cttgggataa agacgcaatc
    19141 atgttttgtg ataattttgg taatagtctt accgtttggc cagatgatat agagtgcgaa
    19201 cttgatttaa gatttgatta tactaaattt attcagaaaa ccattgattg ggcaataaaa
    19261 tataattgtc tacttgtaat agaaaaaaca ggaaatgtag tttcccctaa tataaataat
    19321 ctgatgtatg aaataaaagc atatttggaa agcaagccgt ggcccatatg aaacctaaac
    19381 tcaacaagta ggatgtgtgc ggaacgcacg tatgcggttc tcaaggtttg agctaagagg
    19441 ccgtctgaaa acagaaaaac tgtttcagac gacctttctt ttaaccagtt gccacagcaa
    19501 ccggacaaaa gcagcctacc tccacatcca tataggcaat acaggggaga tattttgtaa
    19561 attctacgaa tattttacct gctaaacagg gtaggatatg gtatgaagcg aacattggct
    19621 taataaacac tatgtcaaga tcgaatcagg ctggtactag attgttgtat tccaattatg
    19681 gattgctata tataacaact gatcattata tctctgcaac acggtttgta gcttggaaat
    19741 aggagtataa cttatgcaat tagagattat cggtagtaaa atttatacgg aacaagattt
    19801 tcataatcaa atttcaaaaa tattttctat acaagattat tatgggaaca atcttgatgc
    19861 tttatgggat ttattaagca caaatgtaga acgaccgatt actttggtat ggaaagatgc
    19921 tatgttctca aaaaatcaat tagaaaatat atttattgaa atcgtaaatg ttctagaaag
    19981 agttaagaaa caagatgagg attatggatt cgaagaaaaa tttaattata ttttagagta
    20041 agtaaaccct aattacatta cgtacacagg cttaaaactc cccagagcca attaagcaag
    20101 ccgtaaccca tataaaactt aaactcaaca agtagcatgt gtgcggaacg tacgcatgcg
    20161 gttcttaaag tttgagctaa gaggccgtct aaaaacagaa aaaccgtttc agacggtctt
    20221 tgtttaacgc caccgatcca gcgggttaca aagcgcagtc aatgccgctg cgccttatgc
    20281 ctccgaagca ataggcagaa catttggaca cggtgaaaac aaaaacgaaa ccgcccaagc
    20341 cgtcggacat ttccttttag gagcagctat tgcccgcgtc aacggtggta attttgctgc
    20401 cggcggctcg gcagcagttg cagctgaaaa ggcggcggaa catcttgccc aacagtataa
    20461 cgacggtaaa accgcaatcg atccgcaaac aggcgagttc aatgccaacc tgctgccgga
    20521 acatatcaaa gaggaaatca aatcaaagag cggggtgatt gcatcgctga cgggcgcggc
    20581 cgtgggcggc acgccggtag atgcgcaaac cggaggtgcg gtcggacaga atgcggtgga
    20641 aaacaacctc tatctgacat cggaagcctt aaagaaggac aagcagacag ctcgtaaaat
    20701 ttattccgtc ataaaagagc aagtcaagca tgaatgcagt tccacaggaa gaattaccga
    20761 atgtcgtcaa aatataggac gcattatcga atttacccaa gacaaacgct ttgacagtag
    20821 gtttaaggac ttaaaaaaag aatccttata ttacctaaat aaacatcctg atttagtagc
    20881 ctcttatttg aaggctgaat acgaaaagct ggatagggaa gacaaaagta tcctgcaccg
    20941 ctacatctca cccggggctg aaatcgtttc gggcagtttg ggggttgttc tttcaggagt
    21001 agccggaggc ggatcttgtg ccgagacttt cggcttaggc tgtgccgccg ctttggttgg
    21061 tgtaacgtct tcctacgatc atgtcattac tgggacgaaa aacttcggaa aaaaagccag
    21121 cgagcaacga ccgacgattg cggttcaggc cttgaagcag ttggggctgt cggagcaggc
    21181 tgcggaatat gttcagttct ctatagattt gttcagtgtg ggtaaatcgg ggggcggtat
    21241 acctaaggct aagcctgtgt ttgatgcgaa accgagatgg gaggttgata ggaagcttaa
    21301 taaattgaca actcgtgagc aggtggagaa aaatgttcag gaaacgagaa gaaggagtca
    21361 gagtagtcag tttaaagccc atgcgcaacg agaatgggaa aataaaacag ggttagattt
    21421 taatcatttt ataggtggtg atatcaataa gaaaggcaca gtaacaggag ggcatagtct
    21481 aacccgtggt gatgtacggg tgatacaaca aacctcggca cctgataaac atggggttta
    21541 tcaagcgaca gtggaaatta aaaagcctga tggaagttgg gaggtgaaaa cgaaaaaagg
    21601 tgggaaagtg atgaccaagc acaccatgtt cccaaaagat tgggatgagg ctagaattag
    21661 ggctgaagtt acttcggctt gggaaagtag aataatgctt aaggataata aatggcaggg
    21721 tacaagtaaa tcgggtatta aaatagaagg atttaccgaa cctaatagaa cagcatatcc
    21781 catttatgaa tagtaatatt tatgaaaaat taggagatta atgatgaaaa gaattaagtg
    21841 cttttgtgat aaatttccat caggagatac atttagaatg tgtatcattc tggatgacta
    21901 tgataatagg gttgattatt atgtaggaat atatgattac attacgtcta ccttaatgag
    21961 cgatatttac tatcgatcca cgattgatga gcatttcaag attatagaat taatagaaaa
    22021 taatccaaat gaaatttatg atgatggcgg tggtcaacaa ttttgcctag aatttcatca
    22081 tgataaggtc attttttatc acaatgaatt tgatgaagaa gatggttatc cagtattaag
    22141 ctgttcgctg catactttta aaactgcttt aattgcttgg aatgcttttt tgcaattgcc
    22201 taaaagtatt cattcggtgg tggagactgt gattgaggaa taagcataat tagcttaatg
    22261 aatagaatca gcgatataga aaggtagaat cagatatatc ccacctaaaa attactctcc
    22321 ttcagcaccg ctaccaaaag gacctaataa tggatatagt gccgggaatt taagcaaaaa
    22381 agtcctgact ttagtttaca taaacggcaa actcaaattc gggccaagca gtctgccgtc
    22441 ttcggcattg aaagcacctg ttcccattgt cggcaaggct gaggctccgc ccggaaaagc
    22501 tgtgcctgtc ggcgaagccc gcattccttc aaatccaaaa gcggtaaaag gaacaaccgt
    22561 taccctgtcg caggaacaac gcctagccaa cggagagctg ataccgaaag gctctgtggt
    22621 aacgtggtcg gacaatgcgg tgaaggttgt gcggcctgat gggaaaaagc ggagatttaa
    22681 tagtcgggat tgggaacaga aggcattgcc gaaaccgcaa ctaccgaaag attacagaga
    22741 tgacatcaac ataggtggaa ataatcctag atttaatcag caacggcatg aaaaaatcga
    22801 atcccacaac ccaaacagaa tagaggagat acgccaacgt aatgccgttt cttctgcaga
    22861 aaggcaggct gatgcattaa aaaggatgca aacgtctggc cgcacccgcc tgcctgggca
    22921 gccacaaaaa ggaaaacccg gcgctgctag agaaatgtcc tcttcagaca atccgaatca
    22981 aacagccgag gcttttgcac gtcgatattt aggcaggaaa cctacactaa tggaacgagt
    23041 cgaaggtgaa aaattatacg gaggtaaatg tgtgggctgt tggaaagcgc aaatgccgga
    23101 cggaaccttt gtggtgtata ggccagcagg taatgcttct atgaccaagg gaactactgc
    23161 gtctgttgat attaataacc caacattaaa taacttaaat attaatttaa aaggcaagcc
    23221 aacagagtta aaatttaaat tccctgctag ataaggatgt tttaatgaat ttagagacag
    23281 cagaaaatat tcaatgcgaa tttgagtatt tagcattaga tggttatttt ccaatgcatt
    23341 ttgcatcgca tggtcaagga aacaaagatt ggcaatttgc agttgaattc atatatagat
    23401 tattaatatg tcaattagca actcttgaac ctatcaactt tgaaacgaaa aatgatattt
    23461 tagacttttg ccataattta gcaaaacaaa gtccttttaa taatgataat gaggtgtggt
    23521 atcaggggga aattgtatta acaaaaaaag ggatagattt aataaaggaa tatattccag
    23581 aggcgtttga agagtggaat ggaaagaaat ttgaattaaa cattcctttc attaaaacat
    23641 taaaaaatat atttgttaat tatgaggtag catgggatga gaataatcca ctattcccca
    23701 ttatatcttt aaacctcggc acctgataaa catggggttt atcaagcgac agtggaaatt
    23761 aaaaagcctg atggaagttg ggaaatgaaa aaaggaaggg gcggaaaacc tatgacaaat
    23821 cattctatgt tcccaaaaga ttgggatgag gctagaatta gggctgaagt tacttcggct
    23881 tgggagaaaa gaaaggaatt agaaggtcaa aaatggagag gtacaagtaa atctggtgtt
    23941 gaaatagaag gatttacgga acctaatcga acagcttatc cactttataa tagtaaatga
    24001 agtattgatt aaaaaggtgt ttttaaatgt ataaaataaa atgtttttat gatcagttta
    24061 gttctggtga attatttaac tactgccagt ttttagataa ttcttctaat caaaagataa
    24121 atacacgagg aacggtgtat caatatatta tttatgtttt aacaggagac ctttatctac
    24181 aaaaagacat agatgaaaat ttagaattta tccatcaagc agaaaataat cccaatgaag
    24241 tttattcagg aggtggacaa ggtttttgct gggatatttc tgctgaaaaa gtagttttct
    24301 atcacaacga atttgatgaa gaagacggct ggcctgattt aagctgttcg ctgcatactt
    24361 ttaaaactgc tttaattgct tggaatgctt ttttgcaatt gcctaaaagt attcattcgg
    24421 tggtggagac tgtgattgag gaataagcat aattagctta atgaatagaa tcagcgatat
    24481 agattggact gcaaatccac gcttatacgc tgtgccatga ttaacaggaa gagagcaact
    24541 tggatgggct agtagggatg gtaagcattt aaatatatca attgatggaa agattacaca
    24601 caaatgacta atttaaaatt agatttctac tctgaagtta ttataaaaga ttcttgccct
    24661 aatgatttgt tagaaaatgg tgagactatt aaaggaaaaa aaggagtggt attaggtata
    24721 agcgaagaag atggtataat ctatggctat actattttac tttttgatat aaaatattgt
    24781 atatatatag ataagaaata tattataccg actggaaaaa aattctctcg cgatgatttt
    24841 tattagctaa tttaagtaat ttaagcaagt tttttagata ctgaaactgg taattatgtt
    24901 tatgtaagca taaggagttc agattgaagg attctacagg gcaaatgagg ctggcaacca
    24961 aggatttggc ggaagccatt aaacgaggag aagtacgtag ttctgctttt acaacaaagc
    25021 aactaaaggc aatcgaaaaa ggagaatcta aaataccgga ttacacttgg catcatcatc
    25081 aagacactgg aaggatgcaa ttgattaatg aagacttgca tcatgatacc ggccatatcg
    25141 gttggagagc gatgagtaaa ggaaagtaa
//
LOCUS       DAXHPG010000010        24890 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXHPG010000010 DAXHPG010000000
VERSION     DAXHPG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24890
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.010"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(91..1038)
                     /locus_tag="ACFM6F_000303"
     CDS             complement(91..1038)
                     /locus_tag="ACFM6F_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC6163789.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(1053..1460)
                     /locus_tag="ACFM6F_000304"
     CDS             complement(1053..1460)
                     /locus_tag="ACFM6F_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC6163790.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYAEILRHAGGNRYEVFYRGT
                     HWQAQNTGQDVFEPGTRALIVRKEGNLLIITHP"
     gene            1541..2200
                     /gene="ung"
                     /locus_tag="ACFM6F_000305"
     CDS             1541..2200
                     /gene="ung"
                     /locus_tag="ACFM6F_000305"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC6163791.1"
                     /translation="MDTWHDALGNEKRQPYFQEILNAVRQERLSGQIIYPPAEDVFNA
                     FRLTAFDRVKVVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDINGFSI
                     PDHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLAAHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            2361..2759
                     /locus_tag="ACFM6F_000306"
     CDS             2361..2759
                     /locus_tag="ACFM6F_000306"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC6163792.1"
                     /translation="MIDVTDFNDEDKLGKGKEMIDRLSRLVGIFKKLNLSSNQAEDDD
                     LLGDAYEYLMRHFATESGKSKGQFYTPAEVSRIMAKIIGISADCRPAPAFMTRPAARV
                     RCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            2672..3305
                     /locus_tag="ACFM6F_000307"
                     /pseudo
     CDS             2672..3305
                     /locus_tag="ACFM6F_000307"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            3653..4597
                     /locus_tag="ACFM6F_000308"
     CDS             3653..4597
                     /locus_tag="ACFM6F_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6163793.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAANLTV
                     VFEETETDKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            4748..4873
                     /locus_tag="ACFM6F_000309"
     CDS             4748..4873
                     /locus_tag="ACFM6F_000309"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163794.1"
                     /translation="MLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT"
     gene            complement(5163..5678)
                     /locus_tag="ACFM6F_000310"
     CDS             complement(5163..5678)
                     /locus_tag="ACFM6F_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163795.1"
                     /translation="MKSKLPFILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(6144..8063)
                     /locus_tag="ACFM6F_000311"
     CDS             complement(6144..8063)
                     /locus_tag="ACFM6F_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6163796.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGRRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIIRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRAE
                     KEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQEE
                     LERIETEIEKQFAER"
     gene            complement(8140..8511)
                     /locus_tag="ACFM6F_000312"
     CDS             complement(8140..8511)
                     /locus_tag="ACFM6F_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC6163797.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLSQ
                     CRTLSDLTPENLLSDVKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            8708..10015
                     /locus_tag="ACFM6F_000313"
     CDS             8708..10015
                     /locus_tag="ACFM6F_000313"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC6163798.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFSLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            10008..10442
                     /locus_tag="ACFM6F_000314"
     CDS             10008..10442
                     /locus_tag="ACFM6F_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163799.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(10501..10770)
                     /locus_tag="ACFM6F_000315"
     CDS             complement(10501..10770)
                     /locus_tag="ACFM6F_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC6163800.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(10952..13402)
                     /gene="lon"
                     /locus_tag="ACFM6F_000316"
     CDS             complement(10952..13402)
                     /gene="lon"
                     /locus_tag="ACFM6F_000316"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC6163801.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHVHVP
                     EGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            13502..13678
                     /locus_tag="ACFM6F_000317"
     CDS             13502..13678
                     /locus_tag="ACFM6F_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HFC6163802.1"
                     /translation="MPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVLPPYFQ
                     NMTQVSHVFFYFSR"
     gene            13653..14756
                     /locus_tag="ACFM6F_000318"
     CDS             13653..14756
                     /locus_tag="ACFM6F_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6163803.1"
                     /translation="MSSFIFPDDGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(14828..16573)
                     /gene="recD"
                     /locus_tag="ACFM6F_000319"
     CDS             complement(14828..16573)
                     /gene="recD"
                     /locus_tag="ACFM6F_000319"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC6163804.1"
                     /translation="MERQTDEFAQAAARAAIRFLERYAGSGSEVLANCTERLFQALQN
                     GHSFIRLKKGEASFLSTLKPVVGESASPLILEGEKLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSHKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAISGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPDFEPAFALTVHKSQGSEY
                     REVWLLPPSAGGSDDADDALSGLSRGLLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(16640..17335)
                     /gene="lolD"
                     /locus_tag="ACFM6F_000320"
     CDS             complement(16640..17335)
                     /gene="lolD"
                     /locus_tag="ACFM6F_000320"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC6163805.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAETAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(17328..18575)
                     /locus_tag="ACFM6F_000321"
     CDS             complement(17328..18575)
                     /locus_tag="ACFM6F_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC6163806.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTAKLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            18780..19058
                     /locus_tag="ACFM6F_000322"
     CDS             18780..19058
                     /locus_tag="ACFM6F_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163807.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSDSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(19114..19731)
                     /gene="recR"
                     /locus_tag="ACFM6F_000323"
     CDS             complement(19114..19731)
                     /gene="recR"
                     /locus_tag="ACFM6F_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC6163808.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(19798..21336)
                     /locus_tag="ACFM6F_000324"
     CDS             complement(19798..21336)
                     /locus_tag="ACFM6F_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC6163809.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKLSFEQEKAAVENELKMKKAVADFN
                     KAKEKLSDDAFNHPASLAEAAKNSSLKVETQETWLSRQDAQMSGLPENLINAIFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(21396..21770)
                     /locus_tag="ACFM6F_000325"
     CDS             complement(21396..21770)
                     /locus_tag="ACFM6F_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC6163810.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQMPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKALFSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            21920..23548
                     /locus_tag="ACFM6F_000326"
     CDS             21920..23548
                     /locus_tag="ACFM6F_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC6163811.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGSRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            23620..24873
                     /locus_tag="ACFM6F_000327"
     CDS             23620..24873
                     /locus_tag="ACFM6F_000327"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC6163812.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYAGFSFHTDKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRSQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT         5744 a         6717 c         6487 g         5942 t
ORIGIN      
        1 tttgaagcgg aatacaacct tgcccgggtt taaataaaaa tgccgtctga accccgaaaa
       61 cagagcttca gacggcattt tcatcacggc ttatttggcg gttttgctgc tgtcgataat
      121 tttcataccg gcagaaatca ggctgccgat gtcggcaaca ttggcgggca taatcagcgt
      181 attgctttct ttggcaagat tgttgaacgc agcgacgtat tgttccgcaa tcttcagatt
      241 gaccgcatcc gcgccgcctt gggtttgaag ggcggcggca atttgacgga tggcttcggc
      301 attggcttcg gcaacaaggc gcagggattc cgcttcgcct ttggcgcggt tgatgcgggc
      361 gattttctcg gcatttgacg cattgaccgc ggcctgggcc tcgccttcgg attgttggat
      421 ttcggcttcg cgctgaccac tggcaaggtt gatttgttcg attttacggc cttcggattc
      481 ggcgatacgg gcgcgttttt cgcgttcggc agtaatttgc gcctgcattg agcgaaggat
      541 ttcttgcggc ggaaccaaat ccttgatttc gtaacggaga actttcacac cccaagctcc
      601 ggcggcttca tcgagggcgg aaacgacggt gctgttgatt tcgtcgcgtt cttcaaacgt
      661 tttgtccaac tccatacgcc cgataacgga acgcagcgtc gtttgggcaa gctgggtaat
      721 cgccataatg tagttgctcg aaccgtatga ggcgagtttg ggatcggtta cttggaaata
      781 gatgatgccg tcaaccgtca gctgggtgtt gtcgcgcgtg atgcagacct ggctgggtac
      841 gtctaaaggg atttctttca gcgaatgacg gtaggcgacg cggtcgataa agggaatcaa
      901 aatattcaaa ccggccgtca aggcgcgatg gaaacgtcca agcctttcga caacgtggac
      961 ttcctgttgt gggatgacaa caaaggattt gaaaccgaaa acggcgacgg ctaccaacaa
     1021 gataatgaaa aattccataa ttcctccgag tgttaagggt gtgtgataat aagaaggttg
     1081 ccttccttgc ggacaatgag ggcgcgcgtt cccggttcaa acacatcctg ccccgtattt
     1141 tgcgcctgcc agtgcgtacc gcgataaaaa acttcgtaac ggttgccgcc tgcgtgccgg
     1201 aggatttcgg catattgccc ggcatccaaa tcctgatatg aatccgtttc aacttttccc
     1261 acggcggtct tggcgtgtac gaaccaaata cccagcgcgg aaagcagagc ggcggtcaag
     1321 acggcggcag gcgtgctgcc ggtcagcccg taagcaatgc ccgaacccgc caaagccgcg
     1381 ctgacaacca aaagataaac cgttcccgtc aataattcga tgattaagac ggcaacagcg
     1441 gcaacaaacc atacagtcat acatttcccc acaaagcgcg tcgtttgaca aaatcacgcg
     1501 atatcagcag tatagccgaa tttgaaagga tagggcagat atggatactt ggcacgatgc
     1561 actgggcaac gaaaaacggc aaccgtattt tcaggaaatt ttaaatgcag tcaggcagga
     1621 acgtttgtcg gggcaaatca tctatccgcc ggcggaggat gtgttcaacg cattccgcct
     1681 gacagcgttt gaccgagtca aagtcgtcat tctcggacaa gacccgtatc acggggcagg
     1741 gcaggcgcac ggtttggcat tttccgtcag gcagggtgtc cgcataccgc cgtctttact
     1801 caatatctac aaagagttgg aaaccgacat caacggtttt tccattcccg atcacggctg
     1861 cctgacggct tgggcggagc agggcgtatt gcttctgaac acggttttga cggtgcgtgc
     1921 aggacaggcg cattcgcacg cccttttagg ctgggaacgc tttaccgata ccgtcatcag
     1981 gcagcttgcg gcgcaccgca agcaccttgt cttcatgttg tggggtgggt atgcacaaca
     2041 aaaagggagg ctgatagaca gtcaaaatca tttgatattg accgcaccgc atccgtctcc
     2101 tctgtcggca tatcgcggtt ttttcggctg ccgccatttt tcacaggcaa acagctattt
     2161 gagtcaacac ggtatcgaac cgataaactg gaagctatga atgccgatat agccgttgcc
     2221 gccggcgtgt taaaatcgcg attgagctgc acgccggtac gacttttgac gacatcgtca
     2281 aactcaaaaa caccgccgac atcggcgacc gcctgaataa gattatcgcc caaattgccg
     2341 aagccaacga cttaaaaggc gtgatcgacg ttaccgactt caacgacgaa gacaaactgg
     2401 gtaaaggtaa ggagatgatc gaccgtttga gcaggcttgt cggcattttt aaaaagctca
     2461 acctttcttc caaccaagcc gaagacgacg atttgttagg tgatgcctac gaatacctga
     2521 tgcgccattt tgcgaccgag tcaggcaaat ccaaagggca gttttacacg cctgccgaag
     2581 tctcccgcat tatggcgaag attatcggaa tcagcgcaga ttgccgtcca gcaccagcgt
     2641 ttatgacccg acctgcggct cgggttcgct gttgctcaaa gccgccgccc aagccggcag
     2701 ccaaatcagc ctttacggtc aggaaaaaga tgtggcaacc gcgtcccttg cccgtatgaa
     2761 tatgattttg cacaacaacg aaaccgccga aatcaacacc gggaacacct tgtccgattc
     2821 gtctttccgt gatgaaaacg acgggcttaa gaccttcgat tttgccgttg ccaatccgcc
     2881 ttatcccgcc cgaaaaaaac ggcgattacg cctttttgct gcatctgctc aaaagcctga
     2941 aaccaagcgg caaaggtgcg attattcttc cgcacggtgt gctgtttcgc ggcaatgccg
     3001 aagcgcgtat tcgcacggaa ttgcttaacc ttgaccttat taaaggcatt atcgggctgc
     3061 ctgccaacct gttttacggc acgggcattc ctgcctgcat catcgtcatc gacaaagaac
     3121 acgcccaaac cgcccaattt gccgaagagg gaacaaacca agttatcagc ggcggcagcg
     3181 tgtttatgat tgacgcatcg cgcggcttca ttaaagacgg caacaaaaac cgtctgcgtg
     3241 agcaagacat tcacaaaatc atcgacactt tcacaaacct cgttacagcc gtatggtgca
     3301 tttaagcgaa atcgcagcac aagattacaa ccttaatctg cctcgctata tcgacagcgg
     3361 agaagtcgaa gacctgcaaa atctcgccgc ctatctatct ttatggcggc atacctgcgc
     3421 acgatatgga cgcattggaa gcctattggc aagttttagg ccgtatgaaa aacgagttgt
     3481 ttgccgaaca cgatggccac tttaccacta tacaacggaa tcgattgcaa atctttccca
     3541 ctctcaacag cttaaaatcc tgcgggattg gtgtggaatt tagggctaat ctagtacagc
     3601 cccaaattta atccactata aaatcgaaag cagccaaatc aaagcccata tattggcgca
     3661 ccccgattac gccgccttca aagccggaca cctggcaaag tttgccgcgt ggcacactca
     3721 aaacgacctt gccgccatcc aaccgggcag gcttatccgg aaatggagcg aaagcctgct
     3781 ggacgcgttc aaacccggca gcctgattga agaatacgat ttctaccaaa tcctgacgga
     3841 ctactgggcg gaaaccctgc aagacgatgt ttatctcatc gcccaagatg gctggaaggc
     3901 ggttaaaaac ctggccgaaa tcaccaaaga aagcgatgaa gccgcgaacc tgaccgtcgt
     3961 ctttgaggaa accgaaaccg acaaaaaagg caaagccaaa accaagcgca tcagcaaaaa
     4021 ataccgcagc gaagtcatcg cccccgagct ggttgcccgc cgctactttt cagacggcat
     4081 cgccaagctg gaagaaaaac aaagcgagct ggaacgccta agccaagaat tggaaaacca
     4141 catagaagaa cacggcggcg aagagggtgc gctgaacgac gtattggatg caaaaggcaa
     4201 actttccgcc aaacttctga aaaccgcatt ggaagaaagc ggcatagaag aaggcgaacg
     4261 ggctgtttta caaaccaccc aaacactgat gacgcaggaa aaagccgcga aagacgcagt
     4321 caaaacccaa atcgaagccc tgaaccttgc cgtattcaaa caattcggcc gactttccga
     4381 agccgaaatc aagcagcttg ccgttcaaga caaatggctt gccgatttac aaagtcgaat
     4441 cgaaaatcgc ttggaaaaca gtattcagca gcttatcagc cgcttgaaca cgctggaaga
     4501 ccgctaccgc agcccgatgg ccgagcttgc ccgagaagtg gaaaagtggc aaagcaaagt
     4561 caatgcccac cttgaaaata tgggttttgg aggctgaaat ggcggcacag acaggctata
     4621 aggcgagcgg gttttgagac ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag
     4681 acatttaggg gatcgcggtt cgggtgtccg caccgcttaa tacgtcgtcg tccacgaact
     4741 gacccatttg ctcgaacgcc atcgcaacgc ccgttttatg gcgcatatgg acaactttct
     4801 cccaaactgg caaagcatca aacaacagct taatgccttg gagttatttg cacaaatata
     4861 taatttaaca taatatacat tatgcgaact atcggaaaca gttgtacgtg tccctgtggt
     4921 ctttccaagt aggaaaatta aagtatggac aatgcggctg aatgtatagc ccggagcatc
     4981 cgcgtatccg aaggtactga acgatatgcc ctgcatccct ttcgatgctt ccatccagcc
     5041 ggtattggga aatacgttga ccttcaatat aatatacagg cagccgacac acaggatatg
     5101 ccgctgcttt ttcatcattt cttctgtcaa atccttggaa cggtcgattt gaaaacacgg
     5161 ctttacatac gtcccaattc tctttgcagt gcttggatat tttgctgcct gtccaataca
     5221 ttgctttgta atgcatttat ttcttgatgg ttgatgttgc cgccctttgc cagacgtgct
     5281 tgtgataaca ttttttgggc ttcagtcagg gctttgcgtt cgttgctcaa ttctgcttcg
     5341 agaatggagc gtctgctgtt gtttacaggt gcttgttgag gcgggggcgt attggatttt
     5401 gccggcttgg atactgtttt gaccggggct ttatatttga cagcctgtcc gccgtttgac
     5461 ggtgatggtg tcggttcgga tgtttggggc gggatatagc gttcgctgct gtagttgccg
     5521 attgggggca aatcggttga gaggcagctt ttggacggct tggtggtgta aacggtttct
     5581 ccgttgattg tgcaggtgta gattttggcc gcattcgcac ccaatgggct tgaaatcagg
     5641 gaaaagttga ttaggatgaa ggggagtttt gatttcataa tgtgcttgat gctcgaataa
     5701 ttatttgatt ttttggtatt ttttgtaatg ctttcttggc gttttacggg cttttctttt
     5761 cggttggttg cttttgattt tctttcgggg ctttggcagc ctgcctgttc tttttcccgg
     5821 caggtttttt gtttttcttt ttactttcta tgtgtatttt ggcttcttaa ctgagttttt
     5881 taattttcag gcggtatccg cctccctgat ggctgctgat tttaggtaaa tccgcatcgg
     5941 cgcacgatcc tgctggggct gatgtatata cgcttttgcc atttgagttg caatggtata
     6001 cggaggcttg cgccgccgcg ccagaaaggg acaggagacc caaggcggca aaaagtctga
     6061 tgtttatacc ggtaaaaggc ggtgataacg cccgaattat accgttattg gcaggcaaag
     6121 ataagcaccc tgcccgcgct tctttatcgc tcggcaaact gtttctcgat ttcagtttca
     6181 atccgttcca attcttcttg acaagccagc caaacctctt cgatttgggc aagttgtgtt
     6241 ttgacttttg ccagctggga taaggtattt tgcaattttt ctttgtttgc ttcaaggtaa
     6301 gcgtcttctt gtgctaaaaa tgcttcacat gccgtctgaa tttcggaaag ctgcgccatt
     6361 tctttttcgg cacggtctat tttctgctgt atcggcttgc cgcgtcgggc tttttcctga
     6421 cggatttgcg cttcgatgcg cttggtgtct ttgcggcttt ggctttgtgc ggatgctgct
     6481 ggtgcggcgg cgttttcctg tgccaaacgc cattggcggt aatcgttcaa atcgccgtcg
     6541 aagttcttca gacggccttt gtcgatcagg aggaagctgt cggtagtcgc ttcaagcagg
     6601 ctgcggtcgt gcgatacgat gattaaggcg ccttggaaac tttgcagagc gagcgtcagg
     6661 gcgtggcgca tatccaagtc cagatggttg gttggttcgt caagcagcag caggttcggc
     6721 ttttgccaga taatcatggc gagggcgagg cgggcttttt ctccgccgga aaaaggttcg
     6781 gttttctgta acgccatatc gccaacaaaa ttgaagccgc cgaggaaatt tcggatttct
     6841 tgttcgcgta cttcgggaga aagctgctga atatgccaaa cagggttttg gtcggcgcgg
     6901 atggtgtcga gttggtgttg ggcaaaatag ccgatattga gtttttcgga acggatgatg
     6961 ctgccggaaa gcaaatcgat tttgcctgcc aaggctttga taaaggtgga tttgccgctg
     7021 ccgttgacac ccaatagccc gtagcgtgcg ccgctttcca gcgacagggt aatgtcgtgc
     7081 aggacagttt tgccttcgta acccaaatca gcgtgttcta gctttaacaa aggattgggc
     7141 agatggtcgg gatggtaaaa ctcaaaggaa aactcgctgt ccagatgcgc gggagcgatg
     7201 cgttcgagct tcgccaaagc cttcatgcgg ctttgcgctt gaacggcttt ggtggctttg
     7261 gctttgaagc ggtcgataaa ggattgcaaa tgtttgattt gcgcctgctg tttgacatag
     7321 gcagcttgtt gttgcgcgag acgctgcgca cgttcgtttt ggtaaaaatc gtaattgccg
     7381 ccgtattgcg tgagtttttg ctgcgataat tcaatggttt gggtagttgc cgcgttgaga
     7441 aaatcgcggt catgggaaat gatgatttgc gtgcagggta aagaagcaag gtggttttcc
     7501 agccacaaga cggtttccaa atccaagtgg ttggtcggtt cgtcaagcaa gagcaaatcg
     7561 gcgcggcaaa tcagggcttg cgcaagattc aggcgcatac gccagccgcc ggaaaaggat
     7621 ttgacggggc ggctgtgttc ttcttgcgaa aaacccagcc cgttcaacaa ttttgccgca
     7681 cgcgccggcg cggtataagc gtcgatttct tccaatttag catgatattc cgcctgcttc
     7741 atgccgtcat tttgcgcttc tgcctgcctc aatgccgtct gaaaagcctg caactcggca
     7801 tcgccctgca aaacgtaatc caaagcggaa atatccaaat cgggcgtttc ttgggaaacg
     7861 gaagcgagcc gccagttttt cggaatcgag acatcgccgc cgtcctgagt gatttcaccc
     7921 ttgattaagg caaacaggct cgatttgccc gttccgtttt tgccgatcaa accgacgcgc
     7981 cgaccgggat tgacggtagc gttggctttg tcgagcagga ctttcaaacc gcgttgcagg
     8041 gtgaggtttt tgatttcaat cataacggaa acatcgtcgg gcgggaaaag cccgtatttt
     8101 acctgaaagt cagtgccgat gccgtctgaa acgggaaatt tacggctgaa gccaagccca
     8161 agccctgcgc ccttccgagt gcaggaaaac caatgtcctg aatgccgaat cggtattcat
     8221 gcattccacg ctgattccga ttcgggaaaa atccgccatg attttgggat ggataaactc
     8281 ctgagccgcg cccgtcccga taatcaatat ttccggatag tcaacaggtt tgacgtcgga
     8341 caacaggttt tccggagtca gatcggacaa ggttcggcat tgcgacaggc agaccgaatc
     8401 cttatgtaca agcacgggtt tatggaaact ttgccccgcc agccggattc cgcccgcacc
     8461 gcattcatat tccgcaaact gtccgtctat cggattttct tcaaacaaca tttttttacc
     8521 ccgttgccgc atcatctaca ccgaaaggga tgcaaaatca gacaaattca tgtaggattg
     8581 gcagatttca tctgacccgc ctgccgattt cagacggcat ttgattcaaa gtgcggcaca
     8641 attatatcgg cagcggatat tttcgtcttt caatatttac atttcagtcg gcttacaagg
     8701 agacacaatg aagccagtaa acatcggtct tttaggtttg ggtacggtcg gcggcggtac
     8761 ggctgccgtg ttgcgggaca acgcggagga aatttcccgt cgcttggggc gcgaaatccg
     8821 tatttctgcc gtgtgcgatt tgagtgaaga aaaagcccga caaacctgcc cgtccgcagc
     8881 ctttgtcaaa gacccgttcg aactggtcgc gcgtgaagac gtcgatgtcg tcgtcgaatt
     8941 gttcggcggt accggcattg ccaaagatgc ggtgttgaaa gccattgaaa acggcaaaca
     9001 catcgttacc gccaacaaaa aactgctcgc cgaatacggc aacgaaatct tctcgctggc
     9061 ggaagaaaaa aatgtcatgg tgcaatttga agccgcagta gcgggcggta tcccaatcat
     9121 caaagccctg cgcgaaggtt tggcagcaaa caggattaaa tccatcgccg gcattattaa
     9181 cggcaccagc aacttcatcc tctccgaaat gcgcgaaaaa ggcagcgcgt ttgccgatgt
     9241 actgaaagaa gcgcaggcat tgggttatgc cgaagccgat ccgaccttcg acatcgaagg
     9301 caacgatgcg ggccataaaa tcaccatcat gagcgcactg gcattcggca cgccgatgaa
     9361 cttttccgcc tgctacctcg aaggcatcag caaactcgac agccgcgaca tcaaatacgc
     9421 cgaagaactt ggctatcgca tcaaactgtt gggcattacc cgcaaaaccg gcaaaggcat
     9481 cgagctgcgc gtccacccta ccctgattcc cgaaagccgc ctcttggcaa acgtcaacgg
     9541 cgtgatgaac gccgtgcgcg tcaacgccga tatggttggc gaaaccttat attacggcgc
     9601 gggcgcgggc gcattgccga ccgcttccgc cgtggttgcc gatatcatcg acatcgcccg
     9661 cctggttgaa gccgataccg cccaccgcgt accgcatctg gcgttccaac ccgcgcaagt
     9721 ccaagcgcaa accatcctac ctatggacga aattaccagc agctactacc tgcgcgtcca
     9781 agccaaagac gaaccgggca cgctggggca aatcgccgcg ctgttggcac aagaaaacgt
     9841 gtccatcgaa gcactgattc aaaaaggcgt gattgatcag accactgccg aaatcgtgat
     9901 tctgacccac agcacggtcg aaaaacacat caagtcggca atcgcagcca tcgaagcact
     9961 ggattgcgtg gaaaaaccga ttaccatgat ccgcatggaa agcctgcatg actgagccga
    10021 aacacgaaat gccgacggaa gagcaggttg ccgcgcgcaa aaaagcaaaa gccaaaatcc
    10081 gcaccatccg catttgggcg tgggtcattt tggcgttgct cgctttaacc gccctgctct
    10141 cccaatgcgc gatgtccaaa ccgcaggcaa aacagaaaat tgtcgagtct tgcgtgaaga
    10201 atattccgtt tgccgaaaaa tggcaaaacg atttgcgggc ccgcggttta gattcaaaca
    10261 atacccgcct cgccgtcgac tactgcaaat gtatgtggga gcagcctttg gacagattga
    10321 gcgagaaaca gattagatcc ttcggcaaac tcggcgcaca agaacagctt gacctgctcg
    10381 gcggcgcaaa tgcctttgaa gcacgtgaca agcagtgtgt tgccgatttg aaatcagaat
    10441 aatgtggacc gataaaaaag ccgattcttt aaagaatcgg cttttttcat aaaaaacggc
    10501 ttacagtgcg tctttcaatg ctttacctgc acggaattta ggcgttttgg cggcggcaat
    10561 ggtcagaggc tcgccggttt tggggttgcg gccttggcgt tccgcacgtt cgcccacgta
    10621 gaaagtaccg aaaccgacca aagtaacggt gtcgccttgt ttcagggcgg tggttactgc
    10681 attggtagtg gcatccaaag ctttttgtgc ggcggctttg gaaatgtcgg cttcttgagc
    10741 aatcgcttcg atcaattcag acttgttcac aatcagtccc ttcctgtctt aaaaaatgat
    10801 gaaatgcccg aatactcggg gtttgtactg cttgagcaac tttcgcttta tagcaattct
    10861 gaaattgccg tgtcaagcaa aaaatacgga atcaccctat ttgacaggct ttcaggacga
    10921 aaccgcattt ttacaacaca tttcctgcgt ttcaatgttt ggttgccctg ctgcggggtt
    10981 ttggttttga agcggattcc gccgccgctt ccgcaccaga aggttctgcc caaggctcag
    11041 gctggctttc caaacccaga gccaatacct cgtctatcca tttgaccgga tggatggtca
    11101 ggccggtttt cacgttttca gggatttctt ccaagtcttt gaggttgtct tttggaatta
    11161 aaacgtgttt gatgccgccg cgaagcgcag ccaacagttt ttccttcaga ccgccaatcg
    11221 gcaggacttc gccgcgcagg gtaatttcgc ccgtcatggc aacatcggcg cgcaccggga
    11281 ttttggtgaa tgcagacacc atcgccaaag tcatggcaat gcccgcactc ggaccgtctt
    11341 tcggcgtcgc accttcggga acatggacat ggatgtcttt tttctcgtaa aaatcaggag
    11401 ccaagcccac tgattccgcg cgggagcgga caaccgacca cgctgcggac acggattcct
    11461 tcatcacatc gcccaactgg ccggtgcact gaatcacgcc cttacccggc aatgctgcgg
    11521 cttcgacggt cagcaattcg ccgccgactt ccgtccacgc caaaccggtt acttgtccga
    11581 tacggttttc gctttcggca acgccgtaat cgaagcggcg cacacccaaa tagtcgtgca
    11641 gatttttctc atttacttta accgccctgg gcttggcttt tccggttttt ttggtttttg
    11701 agtcttcgtc caaggtaatc tgcatcacca ccttgcggca gattttggca atctcgcggt
    11761 cgagcgaacg cacgcccgcc tcgcgggtgt agtaacggat aatatcgcgc accgcgcttt
    11821 cttcgattgc caattcccct tcttttacgc cgttgcgctt catttgcttc ggcacgaggt
    11881 actgcatcgc gatattgatt ttttcgtctt cggtatagcc ggacagacgg atgatttcca
    11941 tacggtcgag cagcggtgtc gggatattca ggctgtttga agtcgcaata aacatcacat
    12001 cgctcaaatc gtaatccact tcggcataat gatcggcgaa cttgttgttt tgctcgggat
    12061 caagcacttc gagcaacgcg ctggcgggat cgccccggaa gtcgctaccc aatttgtcga
    12121 tttcgtcgag caggaacaat gggtttttta cgccggcttt tgccatattc tgcaaaatct
    12181 taccgggcat ggagccgata taggtgcggc ggtgtccccg gatttcgctt tcgtcgcgca
    12241 cgccgcccaa agccatgcgg acatatttcc gccccgttgc tttggcgatg gattcgccca
    12301 aagaggtttt gcccacgcct ggagggccga ccaggcacag aatcgggcct ttgagtttgt
    12361 ccatacgttt ttggacggcg aggtattcca aaatccgttc tttgactttt tccaggccgt
    12421 agtggtcggt atccagcacc aatccggctt tggcgatgtc tttgctgacg cgggattttt
    12481 tcttccacgg cagttcgagc agggtgtcga tgtagttgcg tacgacggtg gattcggcag
    12541 acatcggcgg catcattttg agttttttca gttcggacag gcatttttct tccgcttctt
    12601 tgctcatccc cgcctttttg atgcctgctt ccaaggcatc cagttcgccg ttttcgtctt
    12661 cttcgcccaa ttctttgtgt atcgctttaa tctgttcgtt cagataatat tcgcgctggg
    12721 atttttccat ttggcgtttg acgcgtccgc gtatgcgttt ttcggcctgc ataatgtcga
    12781 gttcggattc cagctgtgcc agcaggaatt ccatccgttt gccgatttcg ggaatttcca
    12841 aaatctgttg gcgttgcgcc agtttcaact gcaaatgcgc tgcgaccgta tcggttagcc
    12901 ggctgttttc ggcaatgccg ttaatgctgc cgataatttc ggcggggatt tttttattga
    12961 gtttggcgta ttgttcaaac tgcgccaaca gggtgcggcg cacggcttcg aggtcggtat
    13021 tgccgcccgt gtcttcttcc acgaccgtct ctatatggga aacgaacaga ccgcccgtgt
    13081 cttcaatggt cagaacacgt ccgcgataca gcccttcgac caatactttt accgtgccgt
    13141 cgggcagttt caacacttgc aggacttgtg cgaccgtacc ggtctgatac aggtcggcgg
    13201 caaccggttc ttctaccgcc gcatcggttt gcgccaacag gaaaaccggc tcctcgcggg
    13261 taatggcgtt ttccagtgcg gcgatggatt tcggcctgcc gacaaacagc ggcagaacca
    13321 tatgcgggta aacgacgaca tcccgcaaag gaagggttgc caaggcggca tattcctcaa
    13381 aatgcttttc tttttgtgtc ataggtactc tcttgtgtct gacagattgc cgattttcgc
    13441 gtacattggg gttgaaggta ttatttcaag catatgtggt ttatttatgg agtttgatgc
    13501 gatgccgtct gaaacattcc ggcttcagac ggcatgggct tggaaagaca aggcgggaac
    13561 aaaaaactgt tctgtgttgc cgctccttgc tgtaccatcc gtatggtttg cggttctgcc
    13621 gccctatttt caaaacatga cgcaggtatc ccatgtcttc ttttattttt cccgatgacg
    13681 ggttcgaatg gcgcaacgaa agtctgcaaa agccgcccaa aggttggcat tatgtccgcg
    13741 aaagcggtgc aaacggcatt ttgaaggctg cctatcaaaa tattgcaact gtctggcagg
    13801 gcgatttcca caatgccaaa caggtgcttt ccgcaatgaa gaagagggtt cgcaaacctg
    13861 ccgccgtccg ttccgatgcg gatatcgccg cccttttcca cgcccaccgt atgaagcagg
    13921 cgcagcagag ccgtattctg aatatgcttg ccgttgaaat ccgccccggt tttgtgttgg
    13981 acaacaaacg cgcgcccgat atacgcgccg ccctgctcga cgtgtacgga gaggcggacg
    14041 gcaaaccgtt tttcctgccg ctcaatctgc tgctggggtt tatgggtgcg cacgagtggc
    14101 ataagaaagg ggttgccgtt ccgcagctgg gcggcagcat acacgttcct ttcggcgtat
    14161 tctcgccgtt gcgcggcgaa tacctcgacc tgctcgccca tgcgccgtca acgggttttc
    14221 agacggcatt cgatatcggg acgggctcgg gcgtgcttgc cgccattttg gcgaaacagg
    14281 gcattccttc cgtcatcggc acggatacca atccgaaagc cgtcgcctgc gcccgtgcca
    14341 atattgcccg tttgggcttt gaaaaacagg ttgagatacg ggaaaccgat ctgtttcccg
    14401 aagggtttgc cgatctgatt gtctgcaatc cgccctggct tcccgccaag ccgacttccg
    14461 ccgtcgaatc cgcgttatac gaccccgaat ctgcgatgct ggctgcgttt ttgcgggatg
    14521 cgccgaaaca tctgaatccc gacggagaaa tccgcctgat catttccgat cttgccgaac
    14581 atctgcacct gcgtccgtcc gattttctgg ataaggcatt tgctcaggcg ggtttgcgcg
    14641 ttgcggatgt tttgaaaacc aaaccggttc acaaaaaagc tgccgatccg aacaatccgc
    14701 tggcttttgc ccgaaacagg gaaactactt tcctgtaccg tttgaaaaag gcataagggg
    14761 cggcggtgcg cttccggcgg caggcatcgg aacgcccgcg ccgccggcac ggcatcattc
    14821 gggcggatta ttcttgtgaa aatacccgct cgagcatact gcccaatgcc gtctgacgcg
    14881 ttttgacggt ggcggcagct tgccggaagg cttcttcccc gccgaaaaat acgaactttt
    14941 ccctcgcgcg ggtaatggct gtgtaaagca gccctctgct caagcccgaa agcgcgtcgt
    15001 ccgcgtcgtc cgaaccgccg gcggagggcg gcagcagcca aacttcccga tattccgaac
    15061 cctggctttt gtggacggtc agcgcgaaag cgggttcgaa atcgggcagg cagcttaccg
    15121 ctaccttttt aaatccgtcc gcatcggcaa aataggcggc aaggctgccc tgccgtccga
    15181 catcttccat aatcagtccg atgtcgccgt tgaacagttc aagcgcgtag tcgttctgtc
    15241 tgatcataat cggttctccg gcgaaatagg cctgatgttc cggtatgttc attttgcggc
    15301 gtacatggct gcaataggct tcgttgaagt cttccgcatc ctgccgccac gctgccagga
    15361 ccacgatatc cgagatgcct gcgtatgcgg cttcgatatt gccgtctttt accgcctgcc
    15421 aataggcttt gtgcgcccgg tacaaccttt cgactcgagc gttcggactg cattccgaat
    15481 gttccagttc gtccggaaac cggtcaaaca atgcccacgc cccctcatcg cccgatatgg
    15541 cggcacgggc aaggcagccg atgccgctgt tgtcgccgaa gcggtggctg aacgacagat
    15601 gggcggtgtt ttgcgccagc acgggcggat ttgcgctgac gctgaaaccg tgttccggaa
    15661 ggaagccggc cagcctttgg tgcgtttctc cgtccaaaac ggttttgtgc gacaaaacgg
    15721 acagcaccgc cccgattccg acggacggga gctggttttc atcccccagc agaatcacgc
    15781 gcgcgccggt tttgaccgct tttaaaagtt gcaacatcaa tgcggtatcc aacatggagg
    15841 cttcatcgac aatcagcaca tcgaacggca gcgggtaaac agggttgaac gccgcctgca
    15901 ttttgggcgg gcgcagcttc agcagtcggt ggacggtttg cccttccagt ttgagcaaat
    15961 ggcggcggac ggcctccggc gcgtcaaaac cgttgattgc acggtgcagc gcgcgcgcca
    16021 tatgtgccgc cgctttgccc gtcggtgcgg caagcgcgat gttgggaaga ttttcgtctt
    16081 caccgcaaat cagcgccagc agtttggcaa ccgttgtcgt tttgcccgtt cccggcccgc
    16141 cggtaatcac cataaaagac tgcaacagtg ccaaggcggc ggcatcgcgc tgcccttcgc
    16201 tgcccgtgcc ttgaaaccat tttgcgaggt tttgccgcgc gcctgccgcg tcgggggcgg
    16261 atgtgccggc tgccgccaaa cgttttattt cggcagccaa atcgtattcc aactgccaca
    16321 tcctgcccaa aaacagtttt tccccttcca aaatcagagg ggaggcagat tccccgacta
    16381 cgggcttgag tgtcgaaagg aaggacgcct cccctttttt caaacggata aacgaatgac
    16441 cgttctgcaa tgcctgaaac aggcgttcgg tacagttagc aagcacttcg ctgcccgaac
    16501 ccgcatagcg ttccaaaaaa cggattgccg cccttgccgc cgcttgggca aattcatctg
    16561 tctgccgttc catcgtattc cttgtcgaaa tgctgtctga aggcgcgagg cttcagacgg
    16621 cgcgtgttgt ttcggctgtt taggcatttg cgccctgttt ggggtgcagg ctgccgtctt
    16681 tcatgaccat cacgcgctcg aagcggccgg cgagttcgtc gtcgtgcgtt acgaccacca
    16741 gccccgttcc caattctgtt ttcagttcca gcatcatatc caaaacattc ctggcgttcg
    16801 cacggtcgag attgccggtc ggctcgtcgg caagcaggca tttgggttgc gtaaccaagg
    16861 cgcgcgcaat ggcggcacgc tggcgttcgc cgcctgaaag ttcgcccgcg cggtgcgtcg
    16921 aacggtgttt cagcccgacc ttttccagca tcgccattgc cgtttctgct gcctcttcac
    16981 ggcttttttt gccgatcaga agcggcatca tcacgttttc cagtgccgaa aattcaggca
    17041 gaagatggtg gaactggtac acgaaaccga gatggcggtt acgtaaatcg cccaaacgcc
    17101 gctggtttaa ggcacgcaaa tcctcgccca tcagcagcac cctgccttca gacggcatat
    17161 ccagcccgcc tagaatatgc agcagcgtcg attttccgct gcccgaagaa ccgatgatgc
    17221 cggtgctttc ccccgcgcgg atttccaaat ccaagccgtg cagcacccga acgtccaaac
    17281 cgccgtcccg atagcgtttg cccacgcctt cgcatttcaa aatcaactca ctcataacgc
    17341 aaagcctccg ccggttgggt ttttgacgcg cgccggctcg ggtagagcgt ggcaacgaaa
    17401 gacagtccca aagaaatgca ggcaatcagg gcaacgtcgc ccatatcaac atcgctgggc
    17461 aggtagtcga taaaataaac ctgcgaattg ataaggtgga caccgagcag gttttcaaaa
    17521 aatgccacga ccctgccgac gttccaaccc aaaagcacgc cgcagaccac acccgccagc
    17581 gtgccgaaaa agcctgaaaa cgcgccctgc accataaaaa tcttcatcac gccggcaggg
    17641 gaaagaccca aagtccgcaa aatcgcaatg tccgcctgct tttccgtaac cgccatcacc
    17701 aaggaagaga caaggttgaa cgccgccacg gcgataatca gcgtcaggat gatgaacatc
    17761 atccgttttt ccaactcgac cgcctcaaaa tagctgcggt tgctgaacgt ccaatcgcgt
    17821 acccaaaccg tatccctttg cgcctccgga atcagctttg ccgtcaaggc gggggcgttt
    17881 tgcgggtcgg cgagtttcag ccgcagcccc gcaacttcct tatccaagcg gtacagtacg
    17941 cgcgcgtctt ggatatgcgt catcgccaat gagttgtcca cttcgtaaac gcccgtctta
    18001 accagaccga ccacggtaaa ctgcttcagg cgcggcacga ctccggcagg ggtaacgttg
    18061 ccctccggcg tgatgacggt aactttattg ccgacttccg cccccaaagc ctccgccaag
    18121 ccgacaccga ggataatgtc aaactcgccc ggaatcaggt cttcaaactt gcctgccggc
    18181 attttgtcgc catattccac cactttgcgt tcttcagacg gcaaaatgcc gcgcatctgc
    18241 acgcccctga tttcgcccgc gtttgccagc agagcctgat tggaaacata gggcgcggca
    18301 gccaaaatac ctttgcggtt ttccgtaaac cgaagcaggt tgcgccaatc cgtatccgta
    18361 ttgtcgatat agccgatttc ggcgtgcggc gcgacattca ggagctgccc gcgtatttct
    18421 ttctgaaagc cgttcataac cgacaagacg acaatcagcg cggttacgcc caaggcgatt
    18481 ccggcaatcg aaaccatcgt gataaacgac ataaagccgt tgcgcttttt cgccctgaga
    18541 tacctcaagc ctatccaagc ctctagagaa aacataacgc taccttaaaa atgtctgcaa
    18601 acgtgccgcc ccggacggcg gtttgggggc ggcggcaaaa gtttattgta ccgtaaaacc
    18661 ccggcagcgt ccgaacgccc gagtgcgggg gacggggcgg cagagcgggc ggaaaacttg
    18721 cacaacgcgt caaactgccc tatcctttcc ttcagaaaaa ccgtttcttg aggaaaacaa
    18781 tgaatatccg aactgctttt gctttgtgcg ccatcgcctt atccgccgct gcggctgcct
    18841 acgccaaaga aatcaaaatc gatgccaaca acacgcctta ttccgaagcc gacgcgcaaa
    18901 agctggcggc aacggcagtc ggtatgggtg ttaaggaacc tatcagcctg aacggcggca
    18961 gcgacagcat taccgtgtcc ggcagcagcg cgacgcagtg cgtgttcaaa gtcggcaacg
    19021 gaggcgcatt gcagattcaa gggctaaact gcaagtaaac cgcccggaaa aatgccgtct
    19081 gaaggcttca gacggcattt tgcattggcg gcgttatgcc ccgccttctt taattaggcg
    19141 gcgttcgtac accgcctgcg ccagcgttcc cgcatcgaca tattccaatt cgccgcccaa
    19201 ggggatgccc tgcgacagcc tgctgacttt gtaaggcagg tttttaaaaa actcggacag
    19261 gacatacgcc gtcgcattgc cttctgcggt aaaagcggtt gcaataatga tttcttcgac
    19321 ttccccgccg cccagccgtt gcgccagcct gtccaatgcg atggcggata cgtccattcc
    19381 cagtgccgta ttgatttgcc ccatcaggac gaaatacagc ccgtcgtggc agtttgccgc
    19441 ttccatattc gacacgtcgg caggcatatg caccaccatc agccgccgcc cgtcgcgtgt
    19501 ttcatcggca caaatatcgc acaatccgcc ttcgcaaaac gtgttgcaca tcgcgcaatg
    19561 gtaaacctgc ttcaatgccg tctgcaaggc atccaccagt ttttcagcct ctttgcgctt
    19621 gtgttggagc aaatgatacg ctatccgctg tgccgatttc ggcccgacgt tgggtaaaac
    19681 cttcagcgcg tcgatcaatc cttggaaggc atcttgtttt ttgtggctca tcatattccg
    19741 ccgtatggga aaacggccgg aatattccga ccgttatttt gtcaacaaaa gtgtcaatta
    19801 ctgaccgtcg ccgttgtcga ccgattgcgc tcctttggtc tgtttgattt tgccgttgaa
    19861 ataacgtatc aacaagtcga aagtattggc agactgctgt tgcgccaaag cctgttttgc
    19921 aagcggaagc tgtgcggcga tatcatccgg cggggttaca gcctgtactt cgacaatcac
    19981 gggtgccggc agaccgatca gcctgacgta ggcgggtttg ccgtttgccg gttttgcttt
    20041 cagcagttcc gcataagcct cgggcggcat ggactgcctt gcctgctgtg cgcccaaaac
    20101 ggacacttcc gaccatttca cgtcaacagc cttgccgccg ttcagttggg taagcacgtc
    20161 ttttgccttg ttttcggcaa gtttggcggc ttcggtacgg atataagcct gacgtaccgc
    20221 gtctttggct tcggcaaacg gcagggtttt ctcttcgcgg acttctttgg cgcggacgac
    20281 ccacgcggtt tcgctgttga tggtcagcac ttcggaattg tgttttttct tcaatacgtc
    20341 gtcgctgaat atggcattga tcaggttttc aggcagtccg gacatttgcg cgtcctgcct
    20401 gctcagccaa gtttcttggg tttcgacttt caaactgctg tttttggcgg cttcggcaag
    20461 cgaagcggga tggttgaacg cgtcatcgct cagcttttct tttgccttat tgaagtcggc
    20521 aaccgccttt ttcattttca attcgttttc gacggcggct ttttcctgct cgaaagaaag
    20581 tttggcttca tttgccggca aacgcgccac gcgctcttca aacgcatttt tcacttccgt
    20641 ttcactgacg gtttgcttgt ctgcaaaatc cttcagattc aaggcgacat attccaattt
    20701 gaccgcctgc ggcagcagat agtctttttt gttcgcatta taaaatttct gcaaatcggc
    20761 ttcagacact ttgacttggg cgatgaactc gtcggggttg aaagtgtgcg aacggatggt
    20821 gcggttgacc tgcgtcagcc tgatcagctg ttccgcctgc gcgtcgccga ccaatacgcc
    20881 gttttggacg aggtttacca aattctgcaa ggcaaactga tcgcggattt cttcgacaaa
    20941 ctggtcttca gacatatggc gttgggaaag gtagcggttt aaaagcgcgt ggtcgaattt
    21001 gccgtttgcg tcgtggaaat tgggatcgtc cacgataatc tgcttgattt gttcggaaga
    21061 aaccgaaatg cccatcagct tcgcgccctg tttcaggtag gcgcgttgca gcagggattg
    21121 gaacaccgcg tcgcgcgaag ggccgccgcc gtccgcctgt tcgttctgta tggcgttgtt
    21181 gatggagtgg tcgctgattt tttcgtcgcc cacttggacg atgtagtcgg cacccggatg
    21241 ggataccgtg ctgaccccga agccgacgaa ggttaatgca atcaggccca aaaggacttg
    21301 ggcgggcgtt ctgtattttt cgatggaatg gaacatattt taaatcggga tatagaatgg
    21361 gaacgggaaa ttcaagtcgg gtattgtaac ggtttttatc cctgtctgca cggggcttgc
    21421 cggttgaaga tgccgtcgta ggtttcttcg ctgaagccgg cgtaaacctt gccgccgcac
    21481 tccaatacgg gacgcttgat caggctcggc atttcggaca tcagtttgac ggcctccgct
    21541 gtcgagaaca gggctttttg ctgtgtttcg gcatcgagtt tgcgccagct tgtcccgcgt
    21601 ttgttgagca gggttgccaa aggcacttgt tccagccacg agcagatttc cgcttcggaa
    21661 ggcatctgtt ttttgaaatc ccgaaactca aactccaagc cgtatccggc aagtcggttt
    21721 ttggcttttt tgaccgtatc gcaatttggg atgccgtgaa ggactatcat ttggaaacct
    21781 tttgtctgaa ataataaaac ggatatttta ctataagtgt ctgaaaattt gcccgtctgt
    21841 ttcagacggc ggggcggtta tgttacaatc cgaaaattcg aaaaatttaa tctcttgttc
    21901 aataaaggct ttaccaatca tgatttctac caacggcatc accatgcagt tcggcgcgaa
    21961 gccgctattt gaaaacgtat ccgtcaaatt cggcgaaggc agccgctacg gtttgatcgg
    22021 cgccaacggc tcaggcaaat ccaccttcat gaaaatcctc ggcggcgatt tggaacagac
    22081 cgccggtgaa gtcgcaattg aaaacggcgt gcgtttgggt aaattgcgcc aagaccagtt
    22141 tgcctacgaa gacatgcgcg tgctggacgt ggtgatgatg gggcataccg aaatgtgggc
    22201 ggcgatgacc gaacgtgatg cgatttacgc caatcccgaa gccaccgaag acgactacat
    22261 gaaagccgcc gaactggaag ccaagttcgc cgaatacgac ggctacaccg ccgaagcgcg
    22321 tgccgccgaa ctgttgagcg gcgtgggcat ttccgaagat ttgcacaatg cgaaaatggc
    22381 ggaagtcgcc ccgggcttca aactgcgcgt attgctggcg caagccctgt tctccaagcc
    22441 ggatgtgctg ttgcttgacg aaccgaccaa taacttggac atcaacacca tccgctggct
    22501 ggaaggcgtg ttgaaccaat acgactccac catgatcatc atctcgcacg accgccactt
    22561 tttgaacgaa gtctgcaccc acatggcgga tttggactac aacaccatca ccatctatcc
    22621 tggcaactac gacgactaca tgctcgcttc cgcccaatcg cgtgagcgcg ccctgaaaga
    22681 caacgccaaa gccaaagaga aactgcaaga gctgcaagag ttcgtcgcac gcttctctgc
    22741 caacaaatcc aaagcccgtc aggcaaccag ccgtctgaaa caggccgaca aaatcaaatc
    22801 ggagatggtc gaagtcaaac cttccacccg tcaaaacccg tatatccgtt ttgaagccga
    22861 tgaaaaagcc aagctgcacc gtcaggctgt ggaagttgaa aaactggcga aacgctttga
    22921 aacccagttg tttaaaaacc tgaacttcat ccttgaagcg ggacaacgcc tcgccatcat
    22981 cggcccgaac ggcgcgggca aatccaccct gctgaaactc ttggccggcg cgtacaaccc
    23041 cgaatattca gacggcctgt taccggacga aggcagcatc aaatgggcgg aaaaagccag
    23101 tgtcggctac tatccgcaag accatgaaaa cgacttcgac gtcgatatgg atttgagcga
    23161 atggatgcgc caatgggggc aggaaggcga cgacgaacaa gtcatccgcg gcactttggg
    23221 gcgtttgctc ttcggcagta acgatgtcgt gaaaaaagtg aaggttctct ccggtggtga
    23281 aaaaggccgt atgctttacg gcaaactgtt gctgttgaaa cccaatgtct tagtcatgga
    23341 cgaaccgacc aaccatatgg acatggaaag catcgaatcc ttgaacatgg cactggaaaa
    23401 atacaacggc acgctgattt ttgtctccca cgaccgtcag ttcgtttcct ccttggcaac
    23461 ccaaatcatc gaactggacg gcaaaggcgg atatgaacac tacttgggcg attacgaaag
    23521 ttacttggag aaaaaaggcg tagcataacc gccggttgga acaatgccgt ctgaagccgc
    23581 ttcagacggc attgttgata actttaaaat aggaagcata tgcagactta cctcgtcggc
    23641 ggcgccgtcc gcgattatct tttgggcttg cccgtcaaag accgcgattg ggtggtcgtc
    23701 ggcgcagacg cacaaaccat gctggcgcaa ggcttccagc cagtcggcaa agactttccc
    23761 gtgtttctcc atcccgaaac acacgaagaa tacgccctcg cccgcaccga gcgcaaaacc
    23821 gccaaaggtt acgccggctt cagtttccac accgacaaag acgttacgct ggagcaggat
    23881 ttgatgcgcc gcgacctgac catcaacgcg atggcgcaag atgcggacgg caagattatc
    23941 gaccctttcg gcggacaacg ggatttggcg gcaggcattt tgcgccacgt ttccccggcc
    24001 ttcgccgaag accccgtccg catcctgcgt actgcccgct ttgccgcgcg ttacaagttt
    24061 gaaatcgccg aagaaaccat aaagctgatg cggcagatgg tggaaaacgg cgaagcggac
    24121 gcattggttg ccgaacgcgt ctggcaggag tttgcgaaag gtttgatgga aaaaaatccg
    24181 cgcaaaatga ttgaagtgtt gcgcgaatgc ggcgcgctca aagtcttgct gcccgaagtc
    24241 aatgccctct tcggcgtgcc gcaacgcgcc gactaccatc ccgaaatcga cagcggcatc
    24301 cataccctga tgacgctgca acgcgccgcc gatatgggtt tgagcctgcc cgaacgctat
    24361 gccgccctgc tgcacgactt gggcaaagcc aaaacaccgt ccgacatcct gccgcgccac
    24421 cacggacacg acctcgccgg tgtcgaaccc gtgcgcgaag tcaatcagcg gctgcgtgcg
    24481 ccgaaacatt gcgccgagct tgccgaactt gtctgccgtt ggcacattat tttccaccaa
    24541 gtcggacagc ttaaaagcca aaccattctg aacgttttga aaaaaaccga cgctttcaga
    24601 cgccccgaac gctttcagac ggcattgaac gtctgcattg ccgacacgca aggccgtctg
    24661 aaccgcgaac acacgcccta cccgcaacgc gcgcactggc tcgccttact cgaagccgcc
    24721 aatcaggcgg attcgggcaa aatcgccgcc gaatgccgct cgcagggaaa agcgcacctt
    24781 atcgccgaac aaatcgaccg ggcgcggctg gcacaaatcg ccccattgca aaaagcgttt
    24841 cgagcggcgc aagacaaaac agaaaaacat taaaacgtcc aatgcagcca
//
LOCUS       DAXHPG010000011        24352 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXHPG010000011 DAXHPG010000000
VERSION     DAXHPG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.011"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(130..279)
                     /locus_tag="ACFM6F_000328"
     CDS             complement(130..279)
                     /locus_tag="ACFM6F_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163813.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAVFSCVSYADVSTHRTIQKAS
                     PYVPS"
     gene            complement(297..494)
                     /locus_tag="ACFM6F_000329"
     CDS             complement(297..494)
                     /locus_tag="ACFM6F_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163814.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            complement(505..1521)
                     /locus_tag="ACFM6F_000330"
     CDS             complement(505..1521)
                     /locus_tag="ACFM6F_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6163815.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            complement(1620..2834)
                     /gene="gltS"
                     /locus_tag="ACFM6F_000331"
     CDS             complement(1620..2834)
                     /gene="gltS"
                     /locus_tag="ACFM6F_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC6163816.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            complement(3052..4458)
                     /locus_tag="ACFM6F_000332"
     CDS             complement(3052..4458)
                     /locus_tag="ACFM6F_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFC6163817.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAANKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAETQAHAIGRPADYNAARKNYMEAAEFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAISNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            complement(4825..6084)
                     /locus_tag="ACFM6F_000333"
     CDS             complement(4825..6084)
                     /locus_tag="ACFM6F_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFC6163818.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            complement(6221..8269)
                     /locus_tag="ACFM6F_000334"
     CDS             complement(6221..8269)
                     /locus_tag="ACFM6F_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFC6163819.1"
                     /translation="MIPAYIPLRSFGIRKIPNITQFLPEYSLNKTVFNAQTIIGWGLR
                     PTTHQARAFAQSHGLPFVALEDGFLRSLGLGVAGYPPFALVYDDIGIYYDTTRPSRLE
                     QLILAANMPSEMLNQAQQAIDLILTHRLSKYNHALDFSDGLVQDSKNIVLVIDQTFGD
                     MAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSGKKQGYLTDLAQQPRVRLL
                     AEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVTFGLPWYAGWGVSDDRHPKIDSL
                     VQTQRRAPRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELY
                     CVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLAGVKLSDDARILAWGNGKEAIVRFAE
                     QHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSIYFNAETPSRLEYILQNQNFDDQ
                     DFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYR
                     NLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQTDILTCLQY
                     ADEVHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGT
                     LIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHRGYFAKKLGKIKQLYRSFK
                     "
     gene            complement(8431..8970)
                     /gene="rfbC"
                     /locus_tag="ACFM6F_000335"
     CDS             complement(8431..8970)
                     /gene="rfbC"
                     /locus_tag="ACFM6F_000335"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6163820.1"
                     /translation="MNIIDTRIPDVKLLEPKVFGDERGFFMETFRDEWFRQNVAERTF
                     VQENHSKSTQGVLRGLHYQTENTQGKLVRVVSGEVFDVAVDMRRDSPTFGHWVGEVLS
                     ADNKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPNAEHSLIWNDATVGIDWPLLGE
                     PSLSAKDLAGKMLAEAATF"
     gene            complement(9025..9900)
                     /gene="rfbA"
                     /locus_tag="ACFM6F_000336"
     CDS             complement(9025..9900)
                     /gene="rfbA"
                     /locus_tag="ACFM6F_000336"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC6163821.1"
                     /translation="MKGIILAGGSGIRLYPITRGVSKQLLPVYNKPMIYYPLSVLMLA
                     GIRDVLVITTPEDNDAFRRLLGDGSDFGIQISYAIQPSPDGLAQAFLIGEEFIGDDSV
                     CLVLGDNIFYGQHFSQSLKEAAASVETKGATVFGYQVKDPERFGVIEFDENFKALSIE
                     EKPKQPKSDWAVTGLYFYDNRVVEFAKQVKPSERGELEITTLNQMYLDDGSLNVQLLG
                     RGFAWLDTGTHASLLEAASFVETIENIQNLQVACLEEIAWRNGWLSSEEIRNLAKPMC
                     KNEYGRYLLRLVGKD"
     gene            complement(10113..11189)
                     /gene="rfbB"
                     /locus_tag="ACFM6F_000337"
     CDS             complement(10113..11189)
                     /gene="rfbB"
                     /locus_tag="ACFM6F_000337"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC6163822.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIRNTRDAVVNVDKLTYAG
                     NLKSLTEVADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIDSAGEF
                     IQTNIIGTFNLLEAARAYRRQMPSEQREAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKP
                     LPVYGDGMQIRDWLFVEDHARALYQVVTEGEIGETYNIGGHNEKANIEVVRTICALLE
                     ELVPNKPQGVARYEDLITYVKDRPGHDVRYAIDAAKIGRELGWQPQETFESGIRKTVE
                     WYLNNQTWWSRVLDGSYNRERLGS"
     gene            complement(11190..12233)
                     /gene="galE"
                     /locus_tag="ACFM6F_000338"
     CDS             complement(11190..12233)
                     /gene="galE"
                     /locus_tag="ACFM6F_000338"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC6163823.1"
                     /translation="MNKKYPKINYIGNKEKIASWICDQPPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDECQQLDLYRKNIGKLDNQYKRALAFTLMRRAMIRKMPYTEDMRPGDTANPYGA
                     SKAMVERMLTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAS
                     GRLPQLSVFGGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAY
                     SVLEIIRAFEAASGLNIPYQIQPRRAGDLACSFADPSHTKQQTGWETKRGLKQMMEDS
                     WRWVSRNPNGYGD"
     gene            complement(12246..13226)
                     /locus_tag="ACFM6F_000339"
     CDS             complement(12246..13226)
                     /locus_tag="ACFM6F_000339"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6163824.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSVPDNFIFVAAKLPSSSRLVTPYRLYWQKLLLKVF"
     gene            complement(13257..15530)
                     /locus_tag="ACFM6F_000340"
     CDS             complement(13257..15530)
                     /locus_tag="ACFM6F_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC6163825.1"
                     /translation="MNITQILSQELSATTAQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPQDVEAAAQGYLNENVPDTKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYRPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYKNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKKHGVKLIAIGNGTASRETDKIAGELVR
                     GMPEMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRNENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGTEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEIGDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(15595..16245)
                     /locus_tag="ACFM6F_000341"
     CDS             complement(15595..16245)
                     /locus_tag="ACFM6F_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6163826.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMHQFEDMDKAYEYYNSLP"
     gene            complement(16242..17039)
                     /locus_tag="ACFM6F_000342"
     CDS             complement(16242..17039)
                     /locus_tag="ACFM6F_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6163827.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(17039..18202)
                     /gene="ctrB"
                     /locus_tag="ACFM6F_000343"
     CDS             complement(17039..18202)
                     /gene="ctrB"
                     /locus_tag="ACFM6F_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HFC6163828.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(18217..19392)
                     /gene="ctrA"
                     /locus_tag="ACFM6F_000344"
     CDS             complement(18217..19392)
                     /gene="ctrA"
                     /locus_tag="ACFM6F_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HFC6163829.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            19527..20660
                     /gene="neuC"
                     /locus_tag="ACFM6F_000345"
     CDS             19527..20660
                     /gene="neuC"
                     /locus_tag="ACFM6F_000345"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFC6163830.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGAAVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            20664..21350
                     /gene="neuA"
                     /locus_tag="ACFM6F_000346"
     CDS             20664..21350
                     /gene="neuA"
                     /locus_tag="ACFM6F_000346"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HFC6163831.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            21351..22400
                     /gene="siaC"
                     /locus_tag="ACFM6F_000347"
     CDS             21351..22400
                     /gene="siaC"
                     /locus_tag="ACFM6F_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HFC6163832.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFLDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYESLFGKIAA
                     CDIRKGAQIKKTDIE"
     gene            22484..23971
                     /locus_tag="ACFM6F_000348"
     CDS             22484..23971
                     /locus_tag="ACFM6F_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HFC6163833.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDYFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
     gene            24216..>24352
                     /locus_tag="ACFM6F_000349"
     CDS             24216..>24352
                     /locus_tag="ACFM6F_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6163834.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YL"
BASE COUNT         6432 a         5722 c         5732 g         6466 t
ORIGIN      
        1 taatgtctcc tcgatatgat ggaacttttt aactttacgg caaattgccg attctatcgt
       61 atttcctttt cggttgaaat cccacccttt agggcggcag gatcagactt tagagcggat
      121 taacaaaaat taggacggaa catagggcga cgctttttgt atcgtcctgt gtgttgaaac
      181 atcagcatag gaaacgcagg aaaagacggc tgttttgcgc ctgcgtaccg tattagtcaa
      241 aatgccgtct gaaaccgata acagggtttc agacggcatt tccgtcaatt ttccctttat
      301 ctgccgaaca ggttggcgat attttccatc tgttcttcgg taaaaccgtt ctcgcccaat
      361 ctggcggcaa ctgcccattc gcccgacaaa ccgtgttccg caccgagctg ccgccatttt
      421 tcgactttgg cttcgtctgt ggttttttcg ggcagcggtt tgacgggaat cctgcttttg
      481 gggacggtag tcatcggttt gcccctaatc gatggaatag ccgcgaagat actgtatccg
      541 ttcgcgcgtc atccgcgtgt cgcattgcag cttgaggtat tcggcgtatt cctgccggtc
      601 tgcctgcgcg gcggcttgtc ggcagttgct gattttttcc tgcgcccact tgcgttgttc
      661 gccgaccaac tctttttgca cgtcggtatc gagtcctccc caaagtttgg taatttcgga
      721 ttccgcacgc tggttttgta cgcgcgcctc ttccacttcg ccccgtgata cggtaacggt
      781 atcggcacgc tcgccgtcgt caggatgcag gatttccggt tcgggcgcgc cttctgcggc
      841 ttggggtacg cccgcatcgc cgccggcggc attgtgttcc aaaatgtctt cgggcgtggg
      901 tttggacggt tcttcttcac gggcttttcc gctcaaaatc ctgaccgcgt cttctttttt
      961 caccgccttg ccgtctatca tcacgatgct cttcacgccg taaggcagca acgcgacaga
     1021 cagcgtttgc gccgccatac cgaccgtgtt gtcgacaaat gccgtctgac cgtctttgac
     1081 gggcaggaag cggacggctg ccgtcaatac gccgtcttta aactcgacat tgccgcccgt
     1141 cttctgccgc acaatatccg acaaagcggt ttccccgtac agcagggggc tgtttgcctt
     1201 ggcatcggca agcgtttcag acggcacggt aatgttcaaa tcggcgatac agaacgtgcg
     1261 cccgccttcc tgcgtttccg aagcgtgttc caaagaaaac gccaaaccgt aggcggcggc
     1321 gataattttg tcggcatcga caaactgcct gccgtcttcg cgcgcgaaag aacgcgcttc
     1381 ctgcgtgagc gtttcctgaa tattgccgcg tatgccttgc aacacggcgg ggttggcgca
     1441 ttccaatgcc ttgggcggtt cttccctgcc gcaagcggca agcagcaggg caaacggcag
     1501 cgcaatgagt ttccgataca tgatgttcct tccaaacggt gcagaccgat agcggctatc
     1561 atagcaaaag cacctgcagg cggtaaacct tgcaggtgct ttgtcggaaa ggcggaaaat
     1621 cagcctttaa agaaattcac aaaaccggtg agaatcgcgg cattaatcaa atcgacgaag
     1681 aacgcgccga ccataggcac aatcaaaaac gccttatgcg acgcgccgaa agtatgcgtg
     1741 acggactgca tatttgccac cgccgtcggc gttgcaccca agccgaaacc gcaatggccg
     1801 gcagccaata ctgccgcatc atagtcgcgc cccataaaga cataggtaac aaaagtcgcg
     1861 tacaaaacca tcaccacggt ttgtacggca agaatcacgg ttacaggccc cgccaaaccg
     1921 gtcagctccc acagtttcaa attcagcaac gccattgcca agaaaagcga aagcgaagca
     1981 ttgccgaaca catcgatggc gcggtcgaac atattgacct tgaatgcggc agtgaggatg
     2041 ttgcggatga ccacgccgcc aaacagacac cacacgaatt tgggcaggtc gaacagatat
     2101 tctttgtcga agccgtccat aatctcggca aacgccaaac acgcggcaaa catggcaagc
     2161 gtttcaacgg cagattccgc cgtaatcagg cgggtgcgtt ttgcctgctc gaacacgtcg
     2221 tccgcgttgt cgtcctgatc ctgttttttg ttttcaaccg gtttgcggcc cattttgttg
     2281 atcaggcggc gcgcaaccgg cccaccgatc aggccgccga acaccagccc gaaagtagcc
     2341 gatgcaatac ccaaaccggt tgcgccgacc aagccgtatt gcgtttcaaa attaggtccc
     2401 cacgcacctg acgtaccgtg tccgcccgtc agcgacaccg aaccggtaat cagaccgatg
     2461 agcgggtcca aacccaaagc cgtagccagt ccgaccccga caaagttttg caccaagata
     2521 aatccgccca caatcgcggt aaaaaccact agcggcaaac cgcccgcctt caaacgggaa
     2581 aaatccgcgc tcaagccgat ggacgtgaag aaaatcagca taaacgcatt ttgcagcggt
     2641 ttctcaaatt tgaagctcac gccgtacgcc tcgtgcagcg caaacaggat aatcgcggca
     2701 accaaaccgc cagcgaccgg ctcgggaata ttgaagtctc gtaagaattt gattttttga
     2761 accagaaatt taccaaccag caacacgagc gtggcggcaa tcagtgtgta ataactgttg
     2821 aattcccatt ccatgttgtt tcacctccta gaaatatttt tccgagcatc cgtttttgtt
     2881 ccggatgtag tgtgtttcaa cgcttggcgt tatccgccga gtgtcatggt attgatgcgc
     2941 ggacaaggtg ttttcagaaa gaaatgaaaa caatggcgtg tccgtgaaaa ataactggca
     3001 aatattaggg gaatagttaa agattgtcaa aaaatgcgag tgtaaatctt ttcagacggc
     3061 cttttgtccg atggcatggt gtgcttcgcg tcgccattgt tctaataggg atattaatga
     3121 ttcggccgaa gtgtggccgt tgctgatggc agcctgcagc cagtagcagg cggtcggaac
     3181 atgacagccg acaccttgtc cgtgatagta aaaacaggcg aggtggtatt gggcggaagg
     3241 gtggttttgt tcggcagcga tggcgtacca gtgtgcagcc gcccgaggat cttgcgccgt
     3301 accgaggccg taatggtaga tgcggcctaa ggcagccgcc gcgtttttat ggtggaactc
     3361 agcagcttcc atgtaatttt tgcgtgcggc attatagtcg gcgggacgac cgatggcatg
     3421 ggcttgtgtt tccgccaaac ggtagatggt ttcggtttgt tgctgttgca gggcggcagt
     3481 tttgatttgt tcgtattgtt gcggatggta cagcgcggcg tcgctgatga gtttttgtgc
     3541 tgcggcggca gaacccagcg cggcagcacg gtggtagtaa tcgtgcgcga tatggttgtc
     3601 agctttgatg ccgaggccgt agcggtagag gtcgcccatg attttcaggg cttcaggatg
     3661 gtttttctcg gcagcggctt tggcatagcg cgccgcttgg aacggatcgc gttcggaaag
     3721 ttcgccggtc agggcgtatt gggccagctt ggcttgtgct tccgcttgct ccttattggc
     3781 ggcacgacga taccattcta aggcttcggg tttgcgttgg gcggcaagaa ggtcggccag
     3841 cagggtttgt gcggcgatgt ggccggcttg tgcggctggt tcgagatagt gttcggcttg
     3901 tgcgtggttg gggctgacac ctgtaccgta gaagtagatt tgaccaagtt gccagcaggc
     3961 ggcagttacg ccgagtgctg ccgctttttc gtattgttcg atggctgttt caacgtcgcc
     4021 ttggcgttga tgatggcgtg ccagatggta atacgcaggt gcggcatatc gggcggcagc
     4081 aagttccgcc cagtagagcg attgtggatc gttgcgttgc gcgtggtaac gcatgaggat
     4141 taattgggct tctgagtgcc cctgctcacc cagccaggca tagtcgttga gaagctgtcc
     4201 ggtcggggtg ccgttgtgtt cgcgttgttg cagcagggag aagagtgctt tgggatggcg
     4261 ttgggccgcg gcggcttcca accatgaaag tgcctgctcc ggttggccgt tttgcagcag
     4321 atggttgccc aatacgaaag cagcttccgc ataaccttgc tctgccgcct gttgcaacag
     4381 gggcagggct tgggcggtat tggattgttc caaaagggcg gcggcttgcc ggtagaggat
     4441 atcaggagcg gcggtcatgg tttgcgggcg gatataaagt ggaagacgga tgctgcggca
     4501 tatgggcggc gataccggtt gagggctttg taaaaatatt gctcgccaca gttttgtaaa
     4561 gtgtattgcc ttgctggctt atctttagga tgtttggctt gttatattgt tttgcctggg
     4621 agtacatcca tattttgtgt gttattaagg cctcagcttg agtcggtatg cgctcatggc
     4681 agctcggtca ttatactgtt tttgtaattt attgttaatg ataaatgcag atttttgtgt
     4741 tttatttaca tggcagtagc taaggtattg attgtttcag gccgtctgaa atgttttcag
     4801 acggcctgaa cttccttcgc taagtcagat tactggtgtg gaagagttgg aggttttttt
     4861 gttggggaaa aacacctgac tgtagaagtt gccgttaatt tgggtcacgt tgaggtggta
     4921 cattcggtag gcatggaaca gctctttgtc aggcggtgtc ggatgattcc aaaattctgc
     4981 caaggtattt tggtcagtaa tgccgggaat atcataatag gcacggccga gaaccttaac
     5041 tggcatattg tgaatcagtc cggacaggcc gctggtgctg ttgatggtga ccatgccgag
     5101 accgtggcgc aggaaaacgg gcagggggac atcatggaca taaatcacgc ggcctttgag
     5161 ttcggggtgt tctttgataa agcgtttaat gtcgcgccag tagtcgataa aaccgcggtc
     5221 catcggatga tgcttgatga tgatgttggt atcggcaggc gcgtgctcgg caaatgaact
     5281 caaaacatgg agcaggaagc tgcggacgct gggaaagtcg caatggatac ggacttggct
     5341 gtcgttgaat acctgtaagg gaacaataaa aaacttgccg tatttgcctg cttccacacg
     5401 tttggcgatt tggatgtctt caatatagta gttcaaacgc ttgaggatgg agagcgacca
     5461 cggtttgagg taatggccgg cattgggtgc gcggtggtgg atgtagtcgg ggtatttgcg
     5521 tggattgcgg aacaactcga tatagtaacg gatagcgttt tttgccatgg gcgtaaaacc
     5581 gccgtgtacc ggcgttggcg ctttatattc ttgctgggca agcttaggga attgttcaag
     5641 aaaaaagtcg gcacggcgcg gcaacgggga aaatgcgttg acgccgtctt tttctaaggt
     5701 gatgtagtag gggcggaaat agccttcttc aaacgcccag aaactggctt ggttttcgtt
     5761 tgcaatgcgt tttgcaatga cgtgataagg gcgtgtgtcg ccaaagcaga caacggcctg
     5821 gatgtgatgt tgagtgatgt attcttgcaa aaactcagga aaggcatcgt agttgtcgtt
     5881 aaaaacaacg gtatgcgctt gagtgggcgg ataaaaatag tcgtcgcctg cattaaagtt
     5941 gaatttatgt acggttttgc cgtttgcagt cagccagtcg gcaaggcgca gaaaaaaatc
     6001 gccgacaggg ccttgaagca gcaggatatt ttctgcgctt tcaagcaggt tttgcaggtt
     6061 atttttgagg acggtctgtt tcatgttgca atgtggtttt gttttttatg taatagtttt
     6121 aggttgaact ttcaagcata cgccaagaga attaacgatg caaaggcaat gatgccgcaa
     6181 tagaggcaag tttatgacgc atcgttaact ttgatggtat ttatttgaaa gatcgatata
     6241 gttgtttgat tttacctaat tttttggcaa agtacccgcg atgtaatccg ttgttgtttt
     6301 tttgcatatt tttttgtcgt atcaggattt gggctgcggt ttctgcattt atggcctgat
     6361 gggtttcggg gtggatgtag tcgggatagt ggatgagcgt gccggcaatc agctgccaaa
     6421 gctcaagtct gcggctacgg cgcggaatgg ggagcagatc ttgggtaagt ccccagcctg
     6481 cgtaaaaagg caggccgtag cagctgactt ttttgccgcg caacaaggct tcaaaaccgg
     6541 tcagcgaagt catggtatgt acttcgtctg cgtattggag acaggtcagg atgtcggttt
     6601 gctcggcggt ttggtcggca tatcgtgcag catcttcagg ggaaatatgg ccgatgcggt
     6661 taccgctgac tacatcggga tgcggtttgt agatgatata ggcattgggg tttcgttcgc
     6721 gtacggtacg gagcaaatcc agattgcggt agatttgggg cgaaccgtag cggatagacg
     6781 catcatcttc aacctggccg ggaacgagga tcacggtttt gtcggttgac ggggcggtga
     6841 agtctgagct gccgacgttg tatttactga tgtggttttc ggtcagcatt ttttgcagct
     6901 tcaaggccgt ctgaaagtct tgatcgtcga agttttggtt ttgtaggatg tattcaagac
     6961 gggacggggt ttcggcattg aaataaatgc tcatatcgtc ggtaacgagc gacagcggcg
     7021 gcactaagtt ggagccgagt ccgaccgagc ggataaagcc gtcttccatg cgcagcaggg
     7081 ggatgtggtg ttgttcggca aagcggacga tggcctcttt gccgttgccc caggcaagga
     7141 tgcgtgcatc gtcggacagt ttgacccctg ccagtttttg ggtggaagag ataaatttca
     7201 gacggcaaga gggtacgtta aagaacggtt tggcaaccgc gcgtttccac aaagacatac
     7261 caacgcaata caactcgcca cgcaatttgt cgtttttgcg tttgaccgtc gccagatagt
     7321 cgatgacatc aaagaggctg cctgcttcgc cggtattggg gttgaggtag cggctgtatt
     7381 gcagataggc tgcagcgaag agctgcagca agttgcgggg ggcgcggcgt tgggtttgaa
     7441 caaggctgtc gattttagga tggcggtcgt cactcacgcc ccatccggca taccacggca
     7501 aaccaaacgt taccaaaggc ttgccgcaca acaaggcttc aaaacccatt tgcgaggtca
     7561 cgcaatacac tttgtccacc gcttgcagca gcgaaatcgg attaatgtct tcagccaata
     7621 aacgcacacg cggctgctgt gccaaatcag taaggtaacc ctgtttcttg ccgctcaata
     7681 catcgggatg ggttttgacc cagatttccg catcaggatt ttcggctaag gccgtctgaa
     7741 acatttggcg gaatgtggct tcatctgcgc cgccatattg aatcgccata tcgccgaaag
     7801 tttgatcaat taccaaaaca atgtttttgc tgtcttgtac gaggccgtct gaaaaatcca
     7861 gagcatggtt atatttagac aaacggtgcg tcaaaatcaa atcaatggcc tgctgcgctt
     7921 ggttaagcat ttcagacggc atgtttgcgg ctaaaatcaa ctgctccaaa cgcgatgggc
     7981 gggttgtgtc gtaatagatg ccgatatcgt cgtaaaccag tgcgaagggc ggataaccgg
     8041 ccacgcctaa gcccagtgag cgtaaaaaac cgtcttccaa ggctacaaac ggcaagccat
     8101 gtgattgtgc aaaagcacgt gcttggtggg tggtcgggcg taagccccag ccaatgatgg
     8161 tttgggcgtt gaatacggtt ttgttaagtg aatattccgg tagaaattgc gtaatattcg
     8221 gaattttacg tataccgaaa gagcgaagag gaatgtaggc aggaatcaca ataaatagga
     8281 aaaaatatta ctgacaatat aatatcttat taacagattt tgtgtaaatt ttctgactat
     8341 ttaaatatag tagaaattta gttagtaggc tagctggtaa aaattaggtc atcttgctac
     8401 aaattaccca cgatgaccta aaagtgaaaa ttaaaatgtg gccgcttcag caagcatctt
     8461 gcctgccaaa tccttggccg ataggcttgg ttctcccaat aaaggccagt cgattcccac
     8521 tgtggcatca ttccaaatta gggaatgttc cgcattcggg ttgtaatagt cggtgcattt
     8581 gtagacgaat tccgcttcat cgctcaacac ataaaatcca tgggcaaacc cttccggcac
     8641 ccataattgg cgtttgttgt ctgccgataa aacctcaccg acccagtggc cgaaagtagg
     8701 actatcacga cgcatatcca cggcaacatc aaagacttcg cctgatacca cgcgcaccag
     8761 tttgccttgt gtattttcgg tttgataatg taaaccgcgt aatacgcctt gggtggattt
     8821 gctgtggttt tcctgcacga aagtgcgctc ggctacattt tggcggaacc attcatcacg
     8881 gaaggtttcc ataaaaaaac cacgctcgtc accgaagact ttgggttcga gcaatttgac
     8941 atcgggaatg cgggtatcga tgatattcat catgttttcc taaaaagcgt ttcagacggc
     9001 ctgaaaactg gtaaaagcaa cctcttaatc tttgccaacc aagcgcaata aatagcggcc
     9061 atattcattt ttacacattg gctttgccaa gtttctgatt tcttcgctgc tcaaccagcc
     9121 attacgccat gcgatttctt ccaaacaggc gacttgtagg ttttgaatgt tttcaatggt
     9181 ttcgacaaac gaggctgctt ccaacaggct ggcgtgtgtg ccggtatcaa gccatgcgaa
     9241 accgcgaccg agcaattgga cattgagcga accatcgtcc aaatacattt gattcaaggt
     9301 ggtaatttcc aattcgccgc gttcagacgg cttcacttgt ttggcaaact cgactacgcg
     9361 attatcgtag aaatagaggc cggttaccgc ccaatcggat ttaggttgtt tcggtttttc
     9421 ttcgattgac agggctttga aattctcatc aaactcaatc acaccgaaac gctcgggatc
     9481 tttcacttga tagccgaata cggtagcacc tttggtttcc acggaagcgg cggcttcttt
     9541 cagggattgg ctgaaatgct ggccatagaa aatattgtcg cccaacacca aacacaccga
     9601 atcatcgccg ataaattctt caccaattaa aaaggcttgc gccaaaccat cggggctggg
     9661 ttggatggcg taggaaattt gaataccgaa atcgctgcca tcacccagta agcggcggaa
     9721 agcatcgttg tcttcggggg tggtaatcac cagcacatcg cggattccgg ccaacatcaa
     9781 caccgacaag gggtaataaa tcatcggttt gttgtacacc ggtagtaatt gctttgatac
     9841 gccgcgggta atcgggtaaa ggcggatacc gctgccgcct gcgagaataa tgcctttcat
     9901 cagtttactt tccttatgta gtttttcagg cggccttcat gccgtctgaa cgtattttta
     9961 ttatttgagg cctttgcaaa gccacagatt tgagtacagt tcgaagttat agcagcgcag
    10021 aaagcgcaga catatcacaa agataggcaa gctttcgagc agcgcataac gaagaaatgt
    10081 gccaaagatg gggagtttgc aaaggtctct atttaactgc ctaagcgttc acgattgtag
    10141 ctgccgtcta acacgcgcga ccaccaagtt tgattgttta aataccattc cactgttttg
    10201 cggatgccgc tttcaaaagt ttcttgcggc tgccaaccca actcacggcc gatttttgcg
    10261 gcatcaatcg catagcgcac atcatggccg gggcggtctt tgacataggt aatcaaatct
    10321 tcataacgcg ccacgccttg cggtttgttc ggcaccaatt cttccagcag ggcgcaaatg
    10381 gtgcgcacca cttcaatatt ggctttttca ttgtggccac caatgttgta agtttcgccg
    10441 atttcgcctt ctgtgaccac ttgatacagc gcacgcgcat gatcttcaac aaacagccag
    10501 tcgcggattt gcataccgtc gccatacacc ggcagtggtt tgccatcaag cgcattcaga
    10561 atcatcagtg gaatcagttt ttcaggaaaa tgataagggc cgtagttatt agaacaattg
    10621 gtcacaatgg tcggcaggcc gtaagtacgc agccatgcgc gcaccaaatg gtcgctggag
    10681 gctttggaag ccgaataagg actggaaggg gcgtaaggcg tggtttcggt aaacaaatca
    10741 tccgtgccgt gcaaatcgcc atagacttca tcggtggaaa tatggtggaa acggaaggct
    10801 tcgcgctgtt cagacggcat ttgccgccgg taggcgcgcg cagcttcaag tagattgaac
    10861 gtgccgatga tattggtttg gataaattct cctgcagaat cgatggagcg gtcgacatga
    10921 ctttccgccg ccaagtgcat cacggcatca ggccggtgtt gtgcgaatac gcggtcgagt
    10981 tcggcgcggt cgcaaatatc cacttgttca aaagcatagc ggggattatc ggctacctcg
    11041 gtcaaagatt tcaaattgcc ggcataagtc agcttatcga cattgacgac agcgtcccgg
    11101 gtgtttcgga taatatggcg gacaacggca gaaccgataa agcccgcgcc gccggtaacg
    11161 aggatggttt ttttgtttgc ggtttgcatt taatccccat atccgttggg gttgcggctg
    11221 acccaacgcc acgaatcctc catcatttgt ttcaaaccgc gtttggtttc ccagccggtt
    11281 tgttgtttgg tatgggacgg gtcggcaaag gaacacgcca agtcgccggc gcgacggggt
    11341 tggatttgat agggaatatt taagccggat gcggcttcaa aggcacggat gatttccaaa
    11401 acggaatagg cgcgccccga acccaagttg aacaaatgta cgccggcaac accgcctttc
    11461 gccttcattg ccgcgatatg cccttctgcc aaatccatca catgaatata gtcgcgcata
    11521 ccggtgccgt cgggggtcgg ataatcgccg ccgaataccg acagttgcgg cagcctgccc
    11581 gaagccactt ggcagatata gggcaaaagg ttgttgggaa tgccgttggg ctgttcgccg
    11641 atcaagccgc tttcatgcgc gccgatcggg ttgaaataac gcaacaaaat cacgctccaa
    11701 cgcggatcgg ctttttggat gtcggttaac atccgttcca ccatcgcttt ggacgcgcca
    11761 taaggattag cggtatcgcc cgggcgcata tcttccgtat agggcatttt acgtatcatg
    11821 gcgcgacgca ttaaagtaaa cgccaaagcg cgtttatact ggttatccag tttccctata
    11881 tttttacggt acaaatcaag ttgttggcat tcatcgtgaa aatagaattt ttcggcataa
    11941 cgctgactca taaaaccggc atgcgggctg cctgaaaaaa tcattgcgac atcatcgtca
    12001 tttaatgttt catggttgtt ttctattaat gctaaagcaa tttggtaatt aattgccaaa
    12061 atatcgttag taatcacacg atagccgcgt tttttggctt cataggcaaa ggaacagcct
    12121 ccactaaata catctgcaac tgtatctaca tcagacggag gctggtcaca aatccaggaa
    12181 gctattttct ctttattacc gatatagtta attttcggat atttcttatt cattttcact
    12241 catttttaaa atactttcag caatagcttt tgccaataaa ggcggtacgg cgttaccaac
    12301 ctgctgctgc tgggcaattt tgctgccaca aaaataaaat tatcagggac agattgtaag
    12361 gcagctaatt cacgaacggt taaagcccga ttctgttcat agtgaaaaac tttgcgcata
    12421 tctcctgtaa tacaaacggc tggtttgttg ctgttgtaac ggatgtattt acggatatca
    12481 cctgttttcg gacgtaatgg ttcaggaata tcgttacggt tacctccatt tttaacaaat
    12541 gccatttttt ctaacatttg tgccgaatga ttcatagctt catgatttgc aacgtgtgga
    12601 ttgctttcgc cagcagccag ttttggaaaa tgtcctattg ctgatccaac agtctgatgg
    12661 gaaatctgca aaggttcggg aaaggaaatt ttgcctttat ccctcctccc gataaatatc
    12721 actcggctac gtatctgagg aacaccgaaa tcggctgcac tcagtatctt acattccacc
    12781 gaataaccga tattctgaaa tgcttgaata atctcaatac gtgttttacc tgaattgtgt
    12841 gtatagagtc gcgctacatt ttccataaca aaaaaatatg gttggacaat tttaactatt
    12901 cggacaaact ctttaaataa atggttgcgt gggtcatctg taaatgtccg tccaatcttt
    12961 cctgccatac taaaaccttg acaaggtggt cctccaataa tcaaatcaac tgcttgtccg
    13021 ttaagacaat tgattaaatc ttgttcggtt agtgtggtta aatctttttg cagtaattga
    13081 tgatggggga agttggtacg gtaagtctga caataatcag actccatttc aacagaaagc
    13141 aattgttgga atccggcttg ttcaaaaccc aaggataggc ctcctgctcc tgagaaaagg
    13201 tcaatatagg taagtggtgt gtgcataagt caaaatccat aaactcttcg attattttac
    13261 cgcttcagct tcgcaaacgc atccgccatc gccgaattgg ctggggcgcg gtcgttgcgt
    13321 tggggtttgc ggccggctgt ccgttcgcgg ctgcggtttt cagacggcat tttatgtttt
    13381 gcgccgcccg gttcgtcatc caagcgcatg gtcagcgcga tgcgttttct ggcggcatcg
    13441 acttccagca ctttcacttt cactacatcg ccggctttca ccacttcgcg cgggtcttgg
    13501 acgaatttat tggatagggc ggagatgtgc accaagccgt cctgatgcac gccgatgtcc
    13561 acgaacgcgc cgaagttggc aacgttggaa accacgccct ctaaaatcat accgacttgc
    13621 aagtcgccga tttcgtggat gccttcggca aacgatgccg tctgaaacgc gccgcgcgga
    13681 tcacggccgg gtttttccag ttcggacagg atgtccaaaa tggtcggcag gccgaagcgt
    13741 tcgtcggtga agtcggacgc tttgatttgc ttcacgcgct cgcggttgcc gatgagttcg
    13801 gcggcgctaa tgccttgttg cgccagcatt ttggcgacga cgggataggc ttcggggtgg
    13861 acggcgctcg cgtccaacgg ctctgtgccg ccgttaatcc gcaaaaagcc tgccgcctgc
    13921 tcgaaggttt tttcgcccaa acgcggtact ttcagcaatt ttttgcggct gtcgaacgcg
    13981 ccgttttcat tgcggtaggc gacgatgttt tgggcaaggg tctgattcaa accggaaatc
    14041 cgcgccaaga gcggggcgga ggcagtattc acgtccacgc cgacggcgtt cacgcagtct
    14101 tcgaccaccg cgtccagcga tttggcgagc tggctttggt tcacgtcgtg ctgatactgg
    14161 cccacgccga tggatttggg gtcgattttg accagctcgg caagcgggtc ttgcagcctg
    14221 cgggcgatgg acaccgcgcc gcgcagggaa acgtccaagt cggggaactc gcgcgctgcc
    14281 agttcggacg cggaataaat cgacgcgccg gcttcggaaa cgacgatttt gtgcagcccc
    14341 atttccggca ttccgcgcac cagttcgccc gcgattttgt cggtttcgcg gctggcggtg
    14401 ccgttgccga tggcgatgag cttcacaccg tgttttttaa tcaggcgcga cagcgttgcc
    14461 aacatattgt tttcttgatg taaatagaca atgacggtat ccagcagctt gcccgtgtcg
    14521 tccaccacgg cacatttcac gccgttcttg tatccagggt ctagacccaa agtggtcagc
    14581 cgtccggcgg gtgcggcgag cagcaagtct ttgagatttc gggcaaacac ggtaatcgcg
    14641 tcggtgtcgg cggcttcttt caaacgattt agggcttcga gttccaacga caaaaagatt
    14701 tttgcgcgcc aagtcagacg cacggtgtcg cgcagccatt tgtggccgtc tgaaaccttg
    14761 aaacggcggg cgatgatttg ctcgtattcg ctttgctgcg taatcggcgt gtcgtcgggc
    14821 cggtatttga gcgcgatgtt caacacgcct tcgttgcggc cgcgcaaaac cgccagcgca
    14881 cgatggctgg gcatcgcacg aataggttcg cggtggtcga aataatcgct gaatttttcg
    14941 ccttcggttt ctttgcctcc aacgacttgc gcgtggattt cggcttcgtt ccacaactta
    15001 tcacgcagcg tgccgatgag ttccgcgtct tcggcaaact gttccatcag aatcgcgcgc
    15061 gcgccgtcca atgcggcttt ggtgtcgggg acgttttcgt tcaggtagcc ctgcgcggcg
    15121 gcttccacat cctgcggctg ctcggcaagc aacacgtccg ccagcggctg caaaccgtgt
    15181 tcgcgcgcga tttgcgcttt ggtgcggcgt ttgggtttgt agggcagata caggtcttcc
    15241 agcgcggttt tgttatcggc ggcttcgatt tgcgccctga ggtcgtctga aagcttgcct
    15301 tgctcttcaa tgctttttaa aacaacggct ttgcgctctt ccaactcgcg caggtattgc
    15361 agccgctcgg caagccggcg cagctgcgta tcgtccaacc cgcccgtggc ttccttgcgg
    15421 tagcgggcga taaagggcac ggttgcgccg tcgtccaaaa gctcgatagc ggcattgatt
    15481 tgcgcggtag tcgcggaaag ttcttgggaa agaatttgag taatgttcat caataaaatt
    15541 ccaacggaca ggccgtctga aatttcagac ggcctgattt aaaaaacaat cgctttaagg
    15601 tagcgaatta taatattcgt aggctttgtc catatcttca aactggtgca tatgcccttt
    15661 ttccagcacc atcgcattat cgcaatattg cttcatggcg ctgtggctgt gcgacaccaa
    15721 gatgatggaa cggtctttgc gcttttcaaa caactcgtac ttacatttat cggcaaaacg
    15781 cgagtcacca actgcaatca cttcgtcaat caaatagcaa tcaaactcta ccgccaacga
    15841 caaagcaaat gccaaacgcg ccttcatacc tgaagaataa cgcttgaccg gctcgtaaag
    15901 atattgcccc aactcggaaa actcttcggt aaagttcttc acataatcga tatcgacatt
    15961 gtaaatccgg cagatgaaac gcaaattgtc cataccggtc agactgcctt gaaacgcacc
    16021 ggagaatgcc aaaggccaag aaatactcat tgtccgcttg atttcacccg tggtcggcgg
    16081 ctcaacgcca ctgatcaaac ggatgagcgt cgatttacct gcaccgttgc ggccgagaat
    16141 accgattttc tcgcccttct ccattttgaa gctaatatcg tgcaagactg tccgccaacc
    16201 ttggcgggtc agatagcgtt tggaaacgtg ttcaactgaa atcattgcgg ctcgactcct
    16261 ttactgaatt tactgaccat cgccaagcca aacaacaaca acaccagatt gcacaatacg
    16321 atataccaag gattttcata ggtaattaca tcgctgccaa aatatccggc acggaacatt
    16381 tctgtgccat gcaccatcgg aatcattaat gcatattctt gtaccttggg cggcaaatta
    16441 tgcacaaaaa agaacgcacc ggataacggc atcatcacaa aagtcaatgt gccccaaatc
    16501 ttgccaaacg gctcgaaatt aaaggcaatc gaacaaatca ccaaacccaa accaatcgca
    16561 aaaaaagcca tcaaaagcca agccatcagc atataaaaca tatctgccgg catttcaatc
    16621 cagccaatcg caatcaatac cgccataatc acaatctgcg caatggttgc accagcaatt
    16681 tccaaaatca tgcgcgccaa gatggtatcc aaaactctta cattgcggtg ataaagcaag
    16741 ctggcatttg aagaaatcga cccaactgcc cgttttgagg cattacgcca catcatcaac
    16801 atcggatagc cagtaatcgc aaatgcgaca atattcaaag ttgaatatcg gtctgccctt
    16861 aaaaatttcc acatcaagac gataacgaat gtcatcagca acggctcaac aaacagccat
    16921 aaaaagccaa tattattgcg accgtaacgg gtgataattt cccgcatcaa cagcgcaccg
    16981 attacgcgcc tttgaatggc taaagattcc caaaatgatg ttttatgcaa ggctttcatc
    17041 agtttttatg ctcacgaatg ctggcagtca acaggctcaa aataccataa accatcaagc
    17101 cgataatcag agtggcaaca atgttgtata accgtttagg ctcatgtgcc aaatccggca
    17161 ggctcggttg cgagatcact tccaaataaa gctgctgacg gtctgcttca accttggcac
    17221 tttccaaaga agtcatggcg gctgccaact gctgctctgc caactggttt tccaaataca
    17281 cacgctgata ttcggcagcc tgattagaca acgaagaatg cccaccgccc gaaatggcac
    17341 gtaactgttg gtcaatttct ttacgcaagc tctgctcacg cgcctgcaaa cccggaatct
    17401 gcggattctc cggagtgact gctttcacct gatccagctg ggtttgaatc acaatcaatt
    17461 catcttgcag cttggaaacc aaccccattt gcacttccga ttgcgctttc aaatcaaaaa
    17521 cgccattggc aatccggtaa tccgtcagat tctgagaggc ttcctttacc cgctctgccg
    17581 ccgttttcac tacttcttcc gcatagcgca ccgtatcagc acgtgcacga tcgttcaact
    17641 ggttaatcaa tgcttcacct tgttttaaca aagcctcatt gattttctta gattccagcg
    17701 catcaaagga agttacattc aacgtggaaa tacccgaaac cgtatcaaaa ttgatcatca
    17761 cctgattttt atagtattga taaaaagcct cttcctcgcc acggaaccca aacccattaa
    17821 agcggctgaa cgcatcacct ttggtttcat aaaactcacg caccggcaag attttacgca
    17881 gttcatccaa agacgagcgc gaacgcatat actccccaac cgtgtaaata tcatcttgcg
    17941 cacgggcaaa acctgtgccc tgcaaaatgg cacccaggcc attgagagaa gattggcttt
    18001 taggcgagcg caccacaaag ctcgattgcg acgtaaaacg atcggaagcg aagaagccga
    18061 aatacaccaa cgaaattacc gtagggataa tcaccgttac ccaaaataaa gggcttagct
    18121 ttttaatcca acttttcttt ttcggctttt tacgctcggc tttggtttca gtggcaactg
    18181 ccacaggaag ttgctcagac atcttaatta ctcacattaa ttagttaaat tattaatact
    18241 gttcgcgcca ctggtaaccg gcgagaacac aaacgacaaa aatttctgca cttcagccaa
    18301 cggcgcattc gacacataca atacatcttt attcttcaca ggaaagcgct gcatagaaaa
    18361 tagcgaatgc gcatcagcca tattcacacg atataccgtt ggaatctctg cctcactgcc
    18421 ataaccttga gcaatccatt tatcctgacg ttctgccggc aattccacca atggcgtata
    18481 gcggaacaca aacacaccac gcgcatcaga acggcgatct tgcaaaccgc ccatacggcc
    18541 aatggcttca gaaagcgata agcctctggc tgaaaaaccg atttcttgtg ttctccccac
    18601 cgcacccata gacgtaaagg tatagggatt ggtaatcatg gtaaccacat caccgcgacg
    18661 cagcaaaata ttttgtcgcg gatttgcaac taaatcttcc aaggcaacag ttcgtactac
    18721 attgccacgt gtcagctgca cattcgtatc ctgcacattt gccgttgaac cacctaccgc
    18781 agccaccgca tccaacacac gctcaccggc tgccgtcagc ggcatacgca cactattccc
    18841 agcacgaatc accgacacat tcgccgcatt attctgcacc aaacgcacca tcacttgtgg
    18901 ctgattggcc atttttttca ggcggccttt aataatttcc tgaacctgac caggcgtttt
    18961 accgaccacc gaaatatcgc caacaaacgg cacagaaacc gtaccacgtg ccgtgaccaa
    19021 ctgctctggc aacttagttt gatgcgcact acccgagccc atcgaagaaa ggccaccacc
    19081 aaacaatact gccggcggcg cttcccaaat cataatatcc aatacatcac caatatttag
    19141 cgtaccagcc gaagcataac catcgccaaa ctgagtgaat gactgattta tctgagcctt
    19201 atataataac tgagcaaccg tatgattcac atcaatcagc tccacttcag gaatttgaac
    19261 ttcagattgt tgccctaaag agacaatttt ttttgcgctg gggcctgatg aaggaatcgc
    19321 agagcatcct acaattaaac ttccacacaa taataatact gcgtgacgaa tataaaattt
    19381 cactttaaac acaagccaaa tcctaatata attataaatg gcctaattat agcacttaat
    19441 cgaaataaat ttatgagtac gtagagtata attagtattc ttctttccaa cttccttata
    19501 cttatatact tatagattct aaaatcatga aaagaattct ttgcattaca ggtaccagag
    19561 ccgacttcgg caagctaaaa cctttattag cctatattga aaatcaccca gaccttgaat
    19621 tgcatttgat tgtaactggt atgcatatga tgaaaacata tggcagaacc tacaaggaag
    19681 taactcgaga aaactatcaa catacatatc tgttttcaaa tcaaatccaa ggtgaaccaa
    19741 tgggtgccgt tttaggcaat accattacgt ttatctctcg tctatctgat gaaattgaac
    19801 ctgatatggt catgattcac ggcgaccgtt tagaagcact agcaggcgca gctgtaggtg
    19861 cattaagcag ccgtttagtt tgccatatcg aaggtggtga actatctggt acagtagatg
    19921 actccattcg tcattctatt agtaaacttt ctcatatcca cttggtagca aatgaacaag
    19981 ctgtcactcg cctagtgcaa atgggagaaa aaagaaagca tattcacatc atcggctccc
    20041 ccgatttaga tgttatggcc tcttccaccc tcccatcctt agaagaagtc aaagaatatt
    20101 acggtttacc atacgaaaat tatggtattt ctatgtttca ccccgtgact acagaagcac
    20161 atttaatgcc acaatatgcg gcccaatatt tcaaagcatt agaattaagt ggccaaaata
    20221 tcattagcat ctaccctaat aatgacactg gcactgaaag tattctgcaa gaattattaa
    20281 aatatcaatc tgataaattt attgcatttc cttccattcg atttgaatat tttttagtct
    20341 tattgaaaca tgctaaattt atggtcggca actcaagtgc aggtattaga gaagctcctc
    20401 tctacggtgt cccttcaatt gatgttggta cacgccaaag taaccgccat atgggaaaat
    20461 ctattattca tacagattat gaaactaaaa atatctttga tgcgattcaa caagcatgca
    20521 gtttaggcaa atttgaagca gatgatacct ttaatggcgg agatactcgc accagcacag
    20581 aaagatttgc tgaagtaatc aacaatcctg aaacgtggaa tgtttctgct caaaaacgtt
    20641 ttatcgattt gaatctttaa attatggaaa aacaaaatat tgcggttata cttgcgcgcc
    20701 aaaactccaa aggattgcca ttaaaaaatc tccggaaaat gaatggcata tcattacttg
    20761 gtcatacaat taatgctgct atatcatcaa agtgttttga ccgcataatt gtttcgactg
    20821 atggcgggtt aattgcagaa gaagctaaaa atttcggtgt cgaagtcgtc ctacgccctg
    20881 cagagctggc ctccgataca gccagctcta tttcaggtgt aatacatgct ttagaaacaa
    20941 ttggcagtaa ttccggcaca gtaaccctat tacaaccaac cagtccatta cgcacagggg
    21001 ctcatattcg tgaagctttt tctctatttg atgagaaaat aaaaggatcc gttgtctctg
    21061 catgcccaat ggagcatcat ccactaaaaa ccctgcttca aatcaataat ggcgaatatg
    21121 cccccatgcg ccatctaagc gatttggagc agcctcgcca acaattacct caggcattta
    21181 ggcctaatgg tgcaatttac attaatgata ctgcttcact aattgcaaat aattgttttt
    21241 ttatcgcccc aaccaaactt tatattatgt ctcatcaaga ctctatcgat attgatactg
    21301 agcttgattt acaacaggca gaaaacattc ttaatcacaa ggaaagctaa atgcaaaaca
    21361 acaacgaatt taaaattggt aatcgttcag taggttacaa ccacgaacca ttgattatct
    21421 gtgaaatcgg catcaatcat gaaggctctt taaaaacagc ttttgaaatg gttgatgctg
    21481 cctataatgc aggcgctgaa gttgttaaac atcaaacaca catcgttgaa gacgaaatgt
    21541 ctgatgaggc caaacaagtc attccaggca atgcagatgt ctctatttat gaaattatgg
    21601 aacgttgcgc cctgaatgaa gaagatgaga ttaaattaaa agaatacgta gagagtaagg
    21661 gtatgatttt tatcagtact cctttctctc gtgcagctgc tttacgatta caacgtatgg
    21721 atattccagc atataaaatc ggctctggcg aatgtaataa ctacccatta attaaactgg
    21781 tggcctcttt tggtaagcct attattctct ctaccggcat gaattctatt gaaagcatca
    21841 aaaagtcggt agaaattatt cgagaagcag gggtacctta tgctttgctt cactgtacca
    21901 acatctaccc aaccccttac gaagatgttc gattgggtgg tatgaacgat ttatctgaag
    21961 cctttctaga cgcaatcatt ggcctgtctg accatacctt agataactat gcttgcttag
    22021 gagcagtagc tttaggcggt tcgattttag agcgtcactt tactgaccgc atggatcgcc
    22081 caggtccgga tattgtatgc tctatgaatc cggatacttt taaagagctc aagcaaggcg
    22141 ctcatgcttt aaaattggca cgcggcggaa aaaaagacac gattatcgcg ggagaaaagc
    22201 caactaaaga tttcgccttt gcatctgtcg tagcagataa agacattaaa aaaggagaat
    22261 tgttgtccgg agataaccta tgggttaaac gcccaggcaa tggagacttc agcgttaacg
    22321 aatatgaatc attatttggt aaaattgctg cttgtgatat tcgcaaaggt gctcaaatca
    22381 aaaaaactga tattgaataa tgcttattaa cttagttact ttattaacag aggattggct
    22441 attacatata gctaattctc attaattttt aagagataca ataatgctaa agaaaataaa
    22501 aaaagctctt tttcagccta aaaagttttt tcaagattca atgtggttga caacatctcc
    22561 attttatctt acccccccac gtaacaattt atttgtcata tctaatttag gtcagcttaa
    22621 ccaagtccaa agcctaatta aaatacaaaa attaaccaat aatttactag taattttata
    22681 tacttctaaa aacttaaaaa tgcctaagtt agttcatcaa tcagctaaca agaatctatt
    22741 tgaatctatt tatctatttg agcttcctag aagccctaat aatataactc ctaaaaaatt
    22801 actttatatt tatagaagtt acaaaaaaat ccttaatatt atacagcctg ctcatctcta
    22861 tatgctgtct tttacaggcc actactccta tctgattagt attgcaaaaa aaaagaatat
    22921 tacgactcat ttaattgatg aagggactgg aacatatgct cctttattag aatcattttc
    22981 atatcatcca acaaaattag aacgttattt gattggaaat aatcttaata ttaaaggata
    23041 tatagattat tttgacatat tgcatgtccc ctttcctgaa tatgctaaaa aaatatttaa
    23101 tgcaaaaaaa tataaccggt tttttgcgca tgctggagga ataagcatta ataataacat
    23161 tgcaaactta cagaaaaaat atcaaatatc taaaaatgac tatatttttg ttagtcaacg
    23221 ctaccccatt tcagatgatt tgtattataa gagtatagta gaaatcttaa acagcataag
    23281 tttacaaatt aaaggaaaga tatttattaa actacaccca aaagagatgg gcaacaacta
    23341 tgtaatgtct ttatttctaa atatggtaga aataaaccct cggctggtag ttattaatga
    23401 acctcctttt ctaattgagc ccctaatata cttaacaaat cctaaaggaa ttataggcct
    23461 ggcctctagt tctttaattt atacaccatt actctcaccc tcaacccaat gtctttctat
    23521 tggagagtta attattaact taattcaaaa atattcaatg gtggaaaaca ctgaaatgat
    23581 ccaagaacac ttagagatta ttaagaaatt taattttatt aatatactaa atgatttaaa
    23641 tggggtaata agtaaccctc tctttaaaac agaagaaaca tttgaaacac ttcttaaatc
    23701 tgcagaattc gcatataaat ctaaaaacta ctttcaggct attttttact ggcaacttgc
    23761 cagcaaaaac aatattacct tattagggca taaagcatta tggtactaca atgcacttta
    23821 taatgtaaaa caaatttata agatggaata ttcagatatt ttttatatcg ataatatctc
    23881 cgtagacttt catagtaaag ataaattgac atgggaaaaa attaaacatt attactattc
    23941 cgccgacaat agaattggta gagatagata aaaatactat gacttctgat ctccatacgt
    24001 atgcttgttt tcaaaaagca gcattaagat agataagtga agcttgatga gagccatttt
    24061 tgtggtcatc gccaaggtac acgcggagag taatcgacaa cagagctgct gtattctggc
    24121 ttttaaagcc cagtagtaaa gtttataagg ctgagacctt tgcaaaatcc cccaaaatcc
    24181 cctaaattcc caccaagaca tttaggagat ttctcatgag caccttcttc cagcaaaccg
    24241 cccaagccat gattgccaaa cacatcgacc gtttcccgct attgaagttg gatcaggtaa
    24301 ttgattggca gccgatcgaa cagtacctga atcgtcaaaa aacccgttac ct
//
LOCUS       DAXHPG010000012        24205 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXHPG010000012 DAXHPG010000000
VERSION     DAXHPG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24205
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.012"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..302
                     /locus_tag="ACFM6F_000350"
     CDS             <1..302
                     /locus_tag="ACFM6F_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6163835.1"
                     /translation="RLPDNDSAYSERWRQIKRHSQYLIGGKLRLWQKRFWEHTIRDEA
                     DFACHFDYLHFNPVKHGYVGQISDWRFSTFHRYVKQGIYPHNWGGGNADFSIEYD"
     gene            374..535
                     /locus_tag="ACFM6F_000351"
     CDS             374..535
                     /locus_tag="ACFM6F_000351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163836.1"
                     /translation="MRWLNLKNSTSVHNTQGIRTKEAVEQGLRGNNRPEKGIGNRWNY
                     DGFIKELAK"
     gene            585..869
                     /locus_tag="ACFM6F_000352"
     CDS             585..869
                     /locus_tag="ACFM6F_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163837.1"
                     /translation="MKVITITLFTSCILSGCYGLEYGMSGGIPHDDWARGSHVSQAKA
                     DAAWKECSDKYMRNGGSGLVEQCMNDKGLIRLSEIRRRQKRAETQRAVIK"
     gene            complement(994..1710)
                     /gene="trmB"
                     /locus_tag="ACFM6F_000353"
     CDS             complement(994..1710)
                     /gene="trmB"
                     /locus_tag="ACFM6F_000353"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC6163838.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTSAQQRAIDTLWG
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1832..3235)
                     /locus_tag="ACFM6F_000354"
     CDS             complement(1832..3235)
                     /locus_tag="ACFM6F_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6163839.1"
                     /translation="MNNPDLLYHQALERLSQEQYDFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPHQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGARPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTTAKNNNPHGQYLLAQYCQYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVYRFQQAAQENDPDAHAALADIYLQGKHLERNHKLALHHAEAAAAERHP
                     EGLRILGDICRYSLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPNQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDNEKACRYLQEAINSGH
                     EQKSFLQELLQQWQNAV"
     gene            complement(3354..5240)
                     /gene="uvrC"
                     /locus_tag="ACFM6F_000355"
     CDS             complement(3354..5240)
                     /gene="uvrC"
                     /locus_tag="ACFM6F_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC6163840.1"
                     /translation="MPSETVFQVIDMNKETRFPEHFDIPLFLKNLPNLPGVYRFFNES
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSESEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCSAPCVGHISEEDYHDSVREASTFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPMPDALKEALESEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLMRRYGKMQEAEANGETVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVVAASKEDLEQVEGISKALAETIY
                     EHLH"
     gene            complement(5304..7466)
                     /locus_tag="ACFM6F_000356"
     CDS             complement(5304..7466)
                     /locus_tag="ACFM6F_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6163841.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLTIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMD
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDSGFDEDDLYRI
                     AAAVEQNATHPLARAIVSAAQTRSLEIPAAQNAQTVVGAGIAAEVEDVGLVKAGKAEF
                     AELTLPEFSDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            7605..8555
                     /gene="trxB"
                     /locus_tag="ACFM6F_000357"
     CDS             7605..8555
                     /gene="trxB"
                     /locus_tag="ACFM6F_000357"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC6163842.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            8711..9079
                     /gene="rpsF"
                     /locus_tag="ACFM6F_000358"
     CDS             8711..9079
                     /gene="rpsF"
                     /locus_tag="ACFM6F_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC6163843.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAAAQ"
     gene            9080..9382
                     /gene="priB"
                     /locus_tag="ACFM6F_000359"
     CDS             9080..9382
                     /gene="priB"
                     /locus_tag="ACFM6F_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC6163844.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGISVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            9389..9619
                     /gene="rpsR"
                     /locus_tag="ACFM6F_000360"
     CDS             9389..9619
                     /gene="rpsR"
                     /locus_tag="ACFM6F_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC6163845.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            9636..10088
                     /gene="rplI"
                     /locus_tag="ACFM6F_000361"
     CDS             9636..10088
                     /gene="rplI"
                     /locus_tag="ACFM6F_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC6163846.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADAQARQEKLDGQTITVAQKAGVDGRLFGSVTNADIAAAI
                     VATGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(10281..11087)
                     /locus_tag="ACFM6F_000362"
     CDS             complement(10281..11087)
                     /locus_tag="ACFM6F_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6163847.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(11088..11834)
                     /gene="pssA"
                     /locus_tag="ACFM6F_000363"
     CDS             complement(11088..11834)
                     /gene="pssA"
                     /locus_tag="ACFM6F_000363"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC6163848.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(11908..12312)
                     /locus_tag="ACFM6F_000364"
     CDS             complement(11908..12312)
                     /locus_tag="ACFM6F_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163849.1"
                     /translation="MANRFQQRKSAYRRLSGKPLFSDGIAANLCGGRKTLSYNLSRFK
                     ISIRSEMQKISFNLLKPANSPKIGKRRPGLNGAPHIPMPSPRYLPASPEMPGNNIHPA
                     KIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            12418..12900
                     /locus_tag="ACFM6F_000365"
     CDS             12418..12900
                     /locus_tag="ACFM6F_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC6163850.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFSELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGALR
                     VDNAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(12952..14163)
                     /locus_tag="ACFM6F_000366"
     CDS             complement(12952..14163)
                     /locus_tag="ACFM6F_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC6163851.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSIGEWGLPSTMFGLFAAGFMYFVFAVLIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            14386..16824
                     /locus_tag="ACFM6F_000367"
     CDS             14386..16824
                     /locus_tag="ACFM6F_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6163852.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            17032..18342
                     /gene="tig"
                     /locus_tag="ACFM6F_000368"
     CDS             17032..18342
                     /gene="tig"
                     /locus_tag="ACFM6F_000368"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC6163853.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAQ
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAMFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            18438..19052
                     /gene="clpP"
                     /locus_tag="ACFM6F_000369"
     CDS             18438..19052
                     /gene="clpP"
                     /locus_tag="ACFM6F_000369"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC6163854.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(19109..20374)
                     /gene="ispG"
                     /locus_tag="ACFM6F_000370"
                     /gene_synonym="gcpE"
     CDS             complement(19109..20374)
                     /gene="ispG"
                     /locus_tag="ACFM6F_000370"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC6163855.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLAFSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(20390..21151)
                     /gene="pilW"
                     /locus_tag="ACFM6F_000371"
     CDS             complement(20390..21151)
                     /gene="pilW"
                     /locus_tag="ACFM6F_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC6163856.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(21154..22248)
                     /gene="rlmN"
                     /locus_tag="ACFM6F_000372"
     CDS             complement(21154..22248)
                     /gene="rlmN"
                     /locus_tag="ACFM6F_000372"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC6163857.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(22392..22817)
                     /gene="ndk"
                     /locus_tag="ACFM6F_000373"
     CDS             complement(22392..22817)
                     /gene="ndk"
                     /locus_tag="ACFM6F_000373"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC6163858.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(22876..24027)
                     /gene="zapE"
                     /locus_tag="ACFM6F_000374"
     CDS             complement(22876..24027)
                     /gene="zapE"
                     /locus_tag="ACFM6F_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC6163859.1"
                     /translation="MSSRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHRRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         5712 a         5866 c         6674 g         5953 t
ORIGIN      
        1 ggcggctgcc ggacaatgat tctgcttatt cggaacgctg gcggcagatc aagcggcaca
       61 gccaatattt aatcggcggc aaactcaggc tttggcaaaa acgcttttgg gaacatacta
      121 tccgcgatga ggccgatttt gcctgccatt ttgattatct gcatttcaat ccggtcaaac
      181 atggctatgt aggacaaatt tccgattggc ggttttctac gtttcaccgt tatgtcaaac
      241 agggtattta tccgcataat tggggtggcg gcaatgcgga cttttctatt gaatacgatt
      301 aaagtaatgt cggattcaag aatccgacct acggaaaact aaagatttta tacgggctac
      361 ggcttgctgg cttatgcgtt ggcttaattt gaaaaacagc acatcggttc ataatacaca
      421 aggtattaga acaaaagaag cagttgaaca gggattgcgt ggtaataata gacctgaaaa
      481 aggaataggt aatagatgga attatgatgg atttataaag gaattagcga agtaacaggt
      541 attctaaata tttctgataa atataaagaa caaggagtaa aaaaatgaaa gtaatcacta
      601 ttacattgtt tactagctgc atactatctg gatgctatgg gcttgaatat ggaatgagtg
      661 gcggcattcc tcatgatgac tgggcaagag gtagccatgt tagtcaagcc aaagcagatg
      721 ctgcgtggaa agaatgttct gacaaatata tgagaaatgg tggttcagga ttagtagaac
      781 aatgtatgaa tgataaagga ttaatacgtt tatctgaaat acgtcgtagg caaaagagag
      841 cagaaacaca aagagcagta attaagtaac aataatgaaa tactactgtt cccgaacaaa
      901 gcggcattta cgcaggtgat gacggctata ttttgaaaaa tgccgtctgt aagcaaacgt
      961 gcttcagacg gcattttttt atccggcggt ctgttatccg atccgtttga ataccaagtc
     1021 ccaaacgccg tgtccgaggc gtttgccgcg cgcttcgaat ttggtttcgg ggcggtagtc
     1081 cggggtgggg gcgtagtctg ccgccgtatt ttgcaggctg tcgaagctgc tgaggacttc
     1141 aagcatctgc tgcgcatatt cttcccagtc tgtcgccaag tggatatagc cgccggtttt
     1201 gagtttgggc agcagtttgg cgatgaaggg ggcttgtatc agacggcgtt tgtggtggcg
     1261 ttttttgtgc cacgggtcgg ggaagaatat gtggatgccg tcgagcgagc cgtcttgcag
     1321 catattttcg acaacttcta cggcatcgtg ccgcatcacg cggatgtttt ccaaatggtt
     1381 ttcttctatg agtttgagca gattgcctac gccgggaccg tgtacgtcga tggcaagaaa
     1441 gtcggtttcg ggcagcctgc gggcgatttc tgcggttgcc gtccccatac cgaagcctat
     1501 ttcgaggatt ttgggtcggc tgcttccgaa acgggcatca agatcggccg gtgttgcttg
     1561 gtagtcgatg ccgaagctgc cccataaggt atcgatggcg cgttgctgcg cggaggtcat
     1621 gtggctttgg cggaggacga aactgcggat gctgcggggg tgttcggctt gccagttgtt
     1681 ttgtgtttgg ttttcggcgg tgtctgtcat aatgctttcg tgcgcttgtg caatcaggat
     1741 gccgtctgaa tgtgaaatgc ctgttcggac ggcgggaaag gaaaggggcg attatacctg
     1801 tttcgtttcg gcagggtagg tgtaacgctg ttcagacggc attttgccac tgttgcaaca
     1861 gttcttgcag aaagcttttt tgttcgtgtc cgctgtttat ggcttcttgc aggtagcggc
     1921 aggctttttc gttgtccggc ccgacaccgt gtccgctgta atggatgcag gcgaggttgt
     1981 agaacgccat actgtctttt tttgcggcgg ctttttcaaa ccatttgcgt gcttcattgt
     2041 agtcggcggt cataccctgt ccgaagtaat acatgctgcc cagattggtt tgggctttgc
     2101 tgtggccgag ttctgcggct tctgtgtaga gtttgagtgc ggctgcatat tcgggcgcgc
     2161 attgtaatcc gtaatgcagg gcttgggctt tttgataaag ccgttctgcc ctttgccgcc
     2221 tgatggcgga atctttaatg ccgccgtatt ggttaggatg gtttaacgcg ctgtcggata
     2281 tgagtttctg ataggcggaa aggctgccgg cttcggctgc ctgccgataa taatgccggg
     2341 ctttttccgt atcgggggct atgcccaaac tgtagcggca gatgtcgccc agtatccgca
     2401 aaccttcggg atggcgttcg gcggcggctg cttcggcatg atgcagggca agtttgtggt
     2461 ttctttccag atgcttgcct tgtaggtaga tgtcggcaag tgcggcatgg gcatcggggt
     2521 cattttcctg tgccgcctgt tggaaccggt aaacggcttc atcgggagcc gtaggtagga
     2581 tgagttcggc aagcggggtg taggcgggaa tgtatccttg ttgtgcggcg gcgcgcaaat
     2641 gattgatagc ttgggtcgtg tcggttggtg taccttgtcc gaaacggtat tgaaggccga
     2701 gctgccattg tgccgccgcc aagccttgtg ctgcagcctc gcggtagaga aggtgtgccg
     2761 tttcaaaatc cggtggcgta ccgtactggc agtattgggc aagcagatat tgtccgtggg
     2821 ggttgttgtt ttttgcggtc gtttttgccc atttcagggc ttcttcaaaa tgtcgggacg
     2881 catacagaag gtacatcagg cggacttgtg cttcgggcag tccttcctct gccaacggtc
     2941 tgtaaagcgg tatcagggtt tccgggcgcg cgccttccaa ttcttggata tatgccagat
     3001 tgtaacgcgc gtagggatgc ccctgttcgg cggcgatgcg gagcatttcc ataccttgtt
     3061 cgcggtcttg gtgcgggctg tccgcgtcca tcaggtgttt tgctaactcg aatgcggcgg
     3121 cgggatgacc tgccgaggcc gcttctgtca gcaggcggcg gacttcggta aagtcatatt
     3181 gttcttgaga caggcgttct aaggcttgat gatagagtaa gtcgggattg ttcatatcag
     3241 atcgttttcc gttttgtcaa atcatcgtta ttcgttatga agttaggttt atatcggcac
     3301 gcctgcctac tgtatcatat tttttacaga cggattttgt ctttgaaagc atgctaatgc
     3361 agatgctcgt aaatggtttc cgccaatgcc ttgctgatgc cttcgacttg ttccaaatct
     3421 tctttgctgg cggcaacgac accgcgcagg ccgccgaagc gggtgagcag ggcctggcgg
     3481 cgtttgctgc ctacgccggg gatgtcgctt aaggaggagg taacgcgtgc tttgtcgcgt
     3541 tttttgcggt ggcctgtaat ggcaaagcgg tgtgattcgt cgcgcacggt ttgcaataga
     3601 tgcaaggccg ggctgttggg cggcaggcgg aagacttcgc cggtaaaagg cagtatgagt
     3661 tcttccatac cggctttgcg ctcagggcct ttggcgatgc cgaccaaggg aatgtgcaga
     3721 ccgagttctt cccatacgga tacggcgacg ccgatttgtc ctttgccgcc gtcaatcaat
     3781 acgacatccg gccatttgac ggtctcgccg ttggcttcgg cttcctgcat tttgccgtaa
     3841 cggcgcatca atacttcgcg catggcggcg tagtcgtcgc cgggcttggc ggtcgtgatg
     3901 ttgtagcggc ggtattgcga aggctggatg ttttgctcat cgtacacgac gcaggacgca
     3961 atagtggcct cgccttgtgt gtggctgatg tcgaaacatt cgaggcggtt gaggccgtct
     4021 gaatccatac cgaggatttt ggcgagttcg tcgatacggt gttgctggct gctttgttgc
     4081 aggcggcgtt gggcaattgc catttgtgcg ttttgctccg ccattttcaa ccagactttg
     4141 cgttcgccta tggttttggt aacgaactgc atttgtttgc cgtgttcgct ctctagggct
     4201 tctttcaatg cgtcgggcat ggggaagttg ctgatgatga tgtcgggttt gcttttgccc
     4261 agatagtgtt gggcgacgaa ggcttcggcg tagtcttgtc cgtttggttc gggatcgttt
     4321 ttggtgtcag ggaaaaaact tttatcgccg acgtgccgtc cgccgcggat gctgacccag
     4381 tgtacgcaga cgatgccgtc tgaaaccgcc agcgcgagca ggtcgatatc gttgggattg
     4441 ttcggatttt tactgtcgat aaactgatta ctctgcatga tgccgagtgc ttggatttgg
     4501 tcgcggtagc gggcggcttc ttcaaactgc aaattcgcgg cggcggtctg cattttgtgt
     4561 tgcagggtgc gggtcagttc gtcggtcttg ccgttgagaa aagtcgaggc ctcgcgcacg
     4621 ctgtcgtggt agtcttcttc gctgatatgg ccgacacagg gtgcgctgca gcgtttgatt
     4681 tgatacagca ggcaagggcg gtcgcggtgt tcgaacacgc tgtcttcgca ggtgcgcagc
     4741 atgaagactt tttgcaggac ttggatgctg tcgcgcacgg cgttgctgtt aggatatggg
     4801 ccgaaatatt gattaggctt tttcagcgtg ccgcggtaat acgccatttg cggatattga
     4861 tggccgctga gcatcaaata aggatagctt ttgtcatcgc ggaaaagaat attgtatttg
     4921 ggcgacaggg ctttgatgaa gttgttttcg agaatcaggg cttcggattc ggagcgggtg
     4981 atggtggttt cgatgtggtg aacctgtttc accatcaatg cgatgcgcgg ggaatggtcg
     5041 tttttctgga aatagccgga cacgcgccgc ttgaggttga cggctttgcc gacgtataag
     5101 acgttgccgc tttcgttgaa aaaacggtat acgcctggca ggttgggcag gtttttgagg
     5161 aaaagtggga tgtcgaagtg ttccggaaaa cgggtttctt tattcatatc aattacttga
     5221 aaaacggttt cagacggcat attaccatat cgctggagac agccttatcc gttcggacaa
     5281 ggctgcaggg cggttacatg ctgctaatcg atttttaccc gtttcaggcg caaggcattg
     5341 ctcaacacgg aaaccgagct tgccgccatt gccgcgccag cgatgacggg atttaaaaag
     5401 ccaagcgcgg cgagaggaat gcccaagata ttgtagaaga aggcgaaaaa caggttttgc
     5461 ttgatgtttt tcaaagtggc ttgcgacacc agcagagcat cggcgagttg gttgaccgaa
     5521 tgctgcatca gcgtggcgga tgcggtatgt tcggcaacgt ccgctccgcc tttcatggcg
     5581 aagctgacgt tagcggcggc aagcgcgggc gcgtcgttga tgccgtcgcc gaccatcgcc
     5641 acggttttgc cggcggcttt gagtttttgc acttcggcag ctttatcgcg cggactcatg
     5701 ttgccgaagg cgtgtgcgat gcccagttgt ttggcgacgt attcgaccgt gccttggttg
     5761 tcgccgctca taatatagac atcgatattg tgttttttca gacggcctat ggcttcggcg
     5821 gtatcggctt tcaacgcgtc ggcaagtgcg aatgcgccga tgggtttgtt atcgaccgag
     5881 actgcaacaa tgcttgcaat gtcccaaacg ccgtctgaaa actcgggcaa ggtaagttcg
     5941 gcaaattcgg ctttgcctgc tttcaccaaa cccacgtctt ccacttcggc ggcaatgcct
     6001 gcgccgacaa cggtttgagc gttttgtgcg gcagggattt ccaagctgcg cgtttgggcg
     6061 gcggaaacga tggcacgggc aagcggatga gtggcgtttt gttcgacggc agcggcgatg
     6121 cggtacaaat cgtcttcgtc aaagccgctg tcgggaacac aataaacggc ggcaacctgc
     6181 ggcctgcctt cggtcagcgt gccagttttg tccaacacaa cggcatcgac gtgggcggct
     6241 tcttccattg ccgctgcgtc tttaaaccaa atgccgtgtt tcactgcttt atccatgccg
     6301 accataatcg cggcgggtgt agccagaccg agcgcacacg ggcaggcaat caccaaaacg
     6361 gcgacggcgt gcatcagcgc aaccgtccaa tcgcccttaa tcagccaagt aacaataaaa
     6421 gtcaacaacg caatgcccac gacggcaggc acgaataccg cagccgcttt atcggctacg
     6481 cgcgcaatcg gtgctttact gccttgtgct tcagagagcg cgttcatcat gtcgccgagc
     6541 tgggtttggc tgccgagctg cgtggcgcgg tacaccacac tgccttcggt cattaacgcg
     6601 cccgccaaca ctttgccgcc cgcctttttt tcttcaggat tggattcgcc ggtaagatgg
     6661 ctctcgtccg cccaaccgct gccgctttca atgatgccgt ctgcggcaat gcgttcgccg
     6721 tggttggcgc ggataaggtc gccgatttgc acttggtcga tgggaagctg tttccattcg
     6781 ccgttgcgtt gcacgttgac ttgggttggt gtaagtttca aaagcaggcc caagctgttc
     6841 aggctggatt ttttggtacg gtgttccaaa aatttgccca gcgacacaaa accgatcacc
     6901 atcacgcctg cttcaaaata cacatgcgcc atgccgtgtg ccgcatgcgg actgaaaaat
     6961 aacatataga cggaatacag ataaatcgat accgtgccga tggtaacgag cacgtccata
     7021 ttcgccaatc cacctttaat actcgcccac gcgcttttgt aaaacgggat tgtcagccaa
     7081 agctgcacca cactcgccaa tgcgaactgc cacaacggcg gaatcatcca atcgtgtctg
     7141 ccgatcatca tccccgccat gccgataagg aacgggacgt tgatggtgaa cagcagccac
     7201 agccgccagc cgatatggtg ttctgcttcg ggttgcggca atgtatcttc cgttttttcc
     7261 ttcgcgccgt aaccggtttt ctcaatgatt ttggcaatgt cggctactga ggttttgctg
     7321 tcgtcaaaca ctacctgcgc ctcctcgctg gcgaagttca ctcccgccga ttcgacaaaa
     7381 tcttttttgt tcaacacttt ttcaatacgc gaagcacatg cctgacaggt catgccttcg
     7441 atttggaaac ggattttttg ttgcataagc gtgattcctg ctttgatatt cagacggcag
     7501 tcaaggttgc gggatgccgt ctgaacgccc gtatggtcga aatttctcaa acgcgccgat
     7561 aattcgagta gaatccgaaa acttgacaca gcaaaggaaa aacaatgtcc caacaccgca
     7621 aactgattat tttgggttcc ggccccgccg gatacaccgc cgctgtctat gccgcgcgcg
     7681 ccaatttaaa ccccgtcatt attacaggta tcgcgcaagg cgggcaactg atgaccacca
     7741 ccgaagtgga caactggcct gccgatgccg acggcgtgca agggccggaa ttgatggcgc
     7801 ggtttctcgc ccacgccgaa cgtttcggaa cggaaatcat ttttgaccaa atcaacgccg
     7861 tcgatctgca aaaacgcccg tttacactca aaggcgatat gggcgagtac acttgcgatg
     7921 ccctgattgt cgcaacaggc gcgtccgcca aataccttgg tttgccgagt gaggaagcgt
     7981 ttgcagggaa aggcgtttcc gcttgtgcca cctgcgacgg tttcttctac aaaaaccaag
     8041 atgttgccgt agttggcggc ggcaatacgg cagttgaaga ggcactctac cttgccaata
     8101 tcgccaaaac cgttaccctg atccaccgcc gcagcgagtt ccgtgccgaa aaaatcatga
     8161 tcgacaaact gatgaaacgc gtggaagagg gcaaaatcat cctcaagctg gaaagcaacc
     8221 tgcaagaagt actgggcgac gatcggggcg taaacggcgc attattaaaa aacaacgacg
     8281 gttctgagca acaaattgcc gtcagcggca tttttatcgc catcgggcac aagccgaata
     8341 ccgatatttt caaaggtcag ttggaaatgg acgaagctgg ttacctgaaa accaaaggcg
     8401 gcacggcgga caatgtcggc gcaaccaata tcgaaggcgt atgggcggcg ggcgacgtaa
     8461 aagaccatac ctaccgtcag gcgattacca gcgcggcttc cggctgtcag gcggctttgg
     8521 acgcggaacg ctggctgggc agccaaaaca tttgaatgcc gtctgaaacc gaaattttcg
     8581 gcggaatgca aatttttcgc accaaatcct tgattccgtc ggcaaacccc aatataatgc
     8641 ctgctctttg aaccttgcct tctgctttcg catggcagaa ggctgttttt accatccata
     8701 aggagataac atgcgtcatt acgagatcgt gtttatcgtt catcctgatc aaagcgagca
     8761 agtgcctgct atggttgaac gttacaaaac catgattgcc gaagcaaacg gtaagattca
     8821 ccgtttggaa gattggggtc gccgccaact ggcttacccg attaacaaaa tccataaagc
     8881 ccattacgtt ttgatgaaca tcgaaaccac tcccgaagtg gttgaagagt tggaaaccgc
     8941 attccgtttc aatgatgcgg tattgcgcca tctaaccatc aaaaccaaac acgccgttac
     9001 cgaagcatcc cctatgttgg gtggtgaaaa ggctaagaac ctgctgagcg gtgcgtctga
     9061 agaagcggct gcccaataat tgggattcaa taatcttgtt tcgcttgccg cgttgattga
     9121 aaaggttttc cctattcgat atacgcctgc cggaatctct gtcttagata ttattttaaa
     9181 gcatgaatca tggcaggagg aaaacgggca gcaatgcctt gtccaattgg aaattccggc
     9241 acggatttta ggcaggcagg cggaagagtg gcagtatcgg caaggtgtat atgttcacgt
     9301 cgaaggtttt ttagctcaaa aaagcagacg ttcccttatg ccgatgctca ggatacaaaa
     9361 tattcaagaa tataaaggtt aaacgacaat ggctcgtcaa tcattcaaac gtagaaaatt
     9421 ctgccgtttc acggctgaaa aaatccaaga agtcgattac aaacaagttg atttgctgaa
     9481 agactttatc tctgaaaacg gtaaaatcat tcctgcacgc atcacaggaa cgaaggcatt
     9541 ctaccaacgc caattggctg ttgccgtaaa acgcgcacgc ttcctggctc tgctgcctta
     9601 caccgaccaa cacaaataat tttggagatt gaatcatgca aattattctg ttagaaaaaa
     9661 tcggcggttt gggcaacttg ggcgacatcg taaccgttaa aaacggctat gcccgcaact
     9721 tcttgattcc tgctggtaag gcaaaacgtg ctactgaagc aaacatgaaa gagtttgaag
     9781 cacgccgtgc cgaattggaa gccaaacagg ccgaaatttt ggcagatgcc caagcccgtc
     9841 aggaaaaatt ggatggccaa accattaccg ttgcacaaaa agccggtgtg gacggtcgtc
     9901 tgtttggttc cgttaccaat gctgatattg cggctgcaat cgttgcgact ggcatcgaag
     9961 ccgtgaaagc aaatgtacgt ctgccgaacg gtcctctgaa agccgttggc gagtacgaag
    10021 tggaagtggc tttgcacact gatgccgttg ctaaaattac cgttgccgtc gttgccgcaa
    10081 ccgagtaatt gaaattatca gatgccgtct gaaaccctgc cgattgtcgg aatgtggttt
    10141 cagacggtat ttgttttgtt ttccgtatat tctgcaaagg tctcggtcag gttgtctgtc
    10201 cttttgtcgg aacaggagac tgggcagctt tgtcctgatt aattacttgt gcaaacggta
    10261 aaaaggtttt ttcgtgtgtt ttaaagcagg ttgtacagca tttttccggc aatcaaaagc
    10321 aacataatgc cgaaggattt tttgagtttg gcagaagaaa gtttgtgggc ggttttgaca
    10381 ccgagcgggg caaaggcaat ggttgccgcg ctgaggacgg cgacggcggg caggtaaagg
    10441 aagcccagtg acccttcggg caatcctgca atattcaggc cgttgagcag atacgatatt
    10501 gcgccggaga gtgcaatcgg ccaggcaagg ccggatgatg tgccgatggc tttatgggcg
    10561 gggaagccgc agtggattaa gaaggggacg gaaagtgaac cgccgcctat gccgacccag
    10621 ctcgacattg tgccgaacag tgtggaaacc gcagtcagtc cgggcagtcc gggcagcggg
    10681 cgggatgccg tctgagggtc ggtatgcagt gttttgaatg cgacggcggt taaaaacagg
    10741 atgaagaaaa tttgaagccc gaacgctggg atatattttg cggagagtgc gccagcgaat
    10801 acgccgaata ccatacccgg catcatcgta aatacggttt tccagtcgac cgcctgtttt
    10861 ttgtgctgcc ccagcatact ggaaaaggcg gtgaagacca tgacggcgaa ggatgtgccg
    10921 acggcgaggt gttgcgcgta aggatgttgt gccaaaccct gcaaatcaag cacccataaa
    10981 acgacaggga caatcagcgt gccgccgcct acgccgaaca ggccggcaat aaaacctgcc
    11041 gcactgccta cggcaagcag gattaagata atgtcccaat gccacattta atccgccttt
    11101 ctgtattttt tccagaatcg gcgtgccgcc ataatgtagc cggacaggct gtatccgaga
    11161 aagaacagga agaggacgag cgacggttcc caagtgacca gaagcagcag taagacggca
    11221 agcagcattc cgacaaaggg gacttgtctg cggatgttga tttctttaaa actccaaaaa
    11281 gggatttgga caatcatcga caggccggca aacagtgtga tgcccaatgc ccaccagtgg
    11341 acggcgggga atttttcgac gctgtggttg acccaaatca gcccgacaat cagcgcggcg
    11401 gcagtcggac tgggcacgcc gataaaccag cgtttgtcca ccttgccgat gagcgtgttg
    11461 aacagcgcaa ggcgcagggc ggcgcaggcg cagtagatga aggcgacgga ataaccgatt
    11521 ttgccgaact gccaaagctg ccatttgtag gcaatcagag cgggagcaac gccgaagctg
    11581 accatatcgg caaggctgtc gagctgctcc ccgaacgcgc tttggctgtt ggtcagccgc
    11641 gccacgcgcc cgtccatacc gtccagcaac atagagatga ataccgcgat ggcggcggtt
    11701 tcataacgtc cgtgcatgga ttgggtgatt gcgtaaaatg cggaaaacag cgcggcgatg
    11761 gtaaaggagt tgggcagcag gtagatactg ttttgacgca gggagcgttg cggggtgttg
    11821 gtcggggttt ccatagctga agtgtctcac ggacgtggag aatcgatagg ggattataac
    11881 ccaaaccatt atcgaacaat acgcccttta ggatgaagcc ctcggaggcg gtttgttttt
    11941 ctgatggcgg atgctttgcg gggcggtttg actctgcctg ccaccgggtg tgttgtttcg
    12001 gatttttgcc ggatggatat tgttgccggg catttctggg cttgccggaa ggtatcgggg
    12061 ggagggcatc ggaatatgcg gcgcaccgtt caaaccgggg cggcgtttcc ctattttcgg
    12121 ggagtttgca ggcttcaaca aattgaaaga tattttttgc atttccgacc gtatgctgat
    12181 tttgaaacgg gataaattat aggacaaggt ttttcgcccg ccgcaaaggt tggccgcaat
    12241 gccgtctgaa aacaacggct tgccgctaag gcggcggtat gcggatttgc gctgctgaaa
    12301 tcggtttgcc atacccggcg gtgcaatctg cggggaggga taagccgata aggcgggact
    12361 tatcctgttt tttgtttata atgcaacaga atttggacga accgtcggga atttatcatg
    12421 atgaaaaaga tactggccgt atcggcacta tgcctgatga ctgcggcggc acgggctgcc
    12481 gatacttacg gctatctcgc cgtttggcag aatccgcaga atgcggacga tgttttgcag
    12541 gttaaaacca caaaagaaga ttcgacgaaa agcgaagcgt tttccgagtt ggaagccttc
    12601 tgcaaaggtc aggacacgct tgcgggcatt gctgaagacg agccgaccgg atgccggtcg
    12661 gtcgtgtcgc tgaacaatac ctgtgtcgcg ctggcatacc cgaaagcctt gggcgcgctg
    12721 cgtgtcgaca acgccgtcgt gattacttct ccgcgtttta cgagcgttca tcaggtcgca
    12781 ctcaaccagt gcatcaaaaa atacggcgta cagggacaat gcggcttgga aacagtgtat
    12841 tgcacatctt cttcttatta cggcggaact gtgcgctctt tgattcaaaa tctcaaataa
    12901 aacggaaaat gccgtctgaa agatgttcag acggcatttc tatatcgacg gtcaggattc
    12961 tttcggatcg ggcagcaggc tgttcaacat aatggcaagt acggcgcaca agcccacgcc
    13021 ggcaaagctg aagctgccca atttgagcgt catgccgccg atgcccgtgg tcagtaccga
    13081 gctgacgatg accaggtttt tcggcagcat caaatcgact ttggcatcaa tcagcgtttt
    13141 cacgcccaaa gaagcaatcg tgccgaacag cagcagcata atgccgccca ttactggcat
    13201 cggaatggaa gccaaaaacg cattgaattt gccgaaaaac gccatgcaga cggcaaaaac
    13261 cgccgcccaa gtcatgatga cggggttgct gtttttggta atcatcaccg cacccgttac
    13321 ttcgccgtag gtcgtaaccg gcgggccgcc gatcagaccc gcaacgcaca cgcccaaacc
    13381 gtcgcctgca agggttttgt ccaagcccgg gtctttcgta tagtctttcc ccgtcacatt
    13441 gccgattgcc atgatgccgc cgatgtgttc gatggcgggg gcgacggcaa cgggcagcat
    13501 aaacagtgcc gcctgccagt tgacctgagg cgtttcaaaa tggggaacgg cgaaccaggg
    13561 cgcgtgtgca atgcttgccg tgtccaccag tcccatcagc agtgccaaaa cataacccga
    13621 agcgacaccg atcaagatag gaatcagctt catcatcctg ctgccgaaaa ccgatacgat
    13681 ggcggtaacg gcaaaggtaa agccggaaag aatcagtgaa tcggtatagt cgatgacctg
    13741 tttgccgtcc gcctgaccca ttgccatgct gcttgccgcc acggcaacag acagaccgat
    13801 gaccatgatg acggggccga tgacgaccgg cggcagcagt ttgtgtaccg ccgccagtcc
    13861 gcgccaacga attagcacgg caaacacaaa atacataaag ccggcggcaa acagtccgaa
    13921 catggtggaa ggcagccccc attctccgat ggagtagata atcggtgcga taaaggcaaa
    13981 cgaagaaccg agaaaaatcg gcactttacg tttggttgta atttggaaca gcagcgttcc
    14041 caagcctgcg cccaaaagcg caagagccgg attcagaccg gtcagcaggg gaaccagcac
    14101 cattgcgccg aatgccacaa ataaaatctg tgcaccggaa acggcaagtt tcagttggtt
    14161 cataccatct tctttcaatc ggttcaaacg gcacggatta tactggtttg ccgagggtct
    14221 gcgtaaaaat acatacgtcg ggcaacagat atgccgtctg aaaggcaatg tctgtctgtt
    14281 gcaggatgaa agcaggagaa atggagtata atccgacact ttccatattc aatccgcaag
    14341 ccgtccggct tccttatttc agacggcatt ttccggtaat acggcatgac agaaaaatcc
    14401 cataaaaaaa cagcaaaagg cagggcggga agcccgtccc caacgtccgc acgcaacaag
    14461 aaagccgaca acggcgcacg gggcaataaa gtttccgagc ggctcaaggc ggtcaaagag
    14521 ttgcagaaaa ccgaaaccaa aaaggcgcgt cccgaacatg tcgtcaacct tatcggcgac
    14581 gcactgtggc tgatgggttt ggcggcaacc ctgtatttgg cgatttccct gatcagtttc
    14641 gatatgggcg atccgtcttg gtcgcacagt tcgccggttg tggaagatgt cgccaattgg
    14701 ggcggactgt tcggcgcgta tgttgccgat gtcggctatt atcttttcgg ctggtcgttc
    14761 tggtggtgga tagcggctgc ctgcgtcgtg ctgtataaaa atttccgcct gcacgcaaaa
    14821 cagacggaaa acgaggcata caaccacaaa atcgctgccg ccgcgctgtt tgtcctgacg
    14881 gtcttcagcc ccgtcttgga gtattttgtg ctgggcggaa aatatgccga ctccctgcct
    14941 gtcggagcag gcggtatggt cggcatacgc gtcggcgcag tgtttgcgtg gctgctgggg
    15001 aaatcgggca gcctgctgat tatcttggtt gttctgctgt tgtcgttgtc cctactggtg
    15061 cagatttcat ggctggaatt tttgaacggt gcaggcaggg cggttcaaaa ccgcctgagt
    15121 gccttatccg gcaaggtcat ggctttagga aaacgccggc cgaataccaa aacagacggt
    15181 gtcgataccc aaaatacacg gcgcatggta aaagaagcca agaatattac ggccaaaccc
    15241 gttgccttgc ccgaaggcag cagcagcaac cgcaaatccg tcgcggtttc cgtcgcgccg
    15301 ccgcccaaaa ttcaggtttc tctgtttgaa gatgacgaac ctcggcaggc gggcgaatac
    15361 cacaagccta cattgaacct attgcggatt cccgacagcg agcccgtcag catcaatccc
    15421 gccgaattgg agcgcactgc cgaactaatc gaatccaagc tggcagaatt cggcatcggc
    15481 gtacaagtcg tatccgccac atccggcccc gtcatcacgc gctacgaaat cgaacccgcg
    15541 caaggtgtca aaggcagcca aattgttgcc ttgtcgaaag atttggcacg ctctatgtcg
    15601 ctgcaatccg tgcgtatcgt cgaaaccatc gccgggaaaa acacgatggg catcgagttg
    15661 cccaacgaca aacgccaaga cgtgatgttg agcgaaatct tgtcctcgcc cgtgtttgcc
    15721 gaagccaaat ccaagctgac cgtcgcgctg ggcaaagaca ttgccggtac ccccgttgtc
    15781 ggcgacttgg cgaaaatgcc gcaccttttg gtcgccggta tgactggttc gggcaagtcc
    15841 gtcggcgtga acggcatgat tatgtctatg cttttcaaag ctacgcccga cgaagtccgc
    15901 ttcattatga tagacccgaa aatgctcgag ttgagcattt acgacggcat tccgcacctg
    15961 ctctgtcccg tcgtgaccga tatgcgcgaa gcagggcagg cgttgaactg gtgcgtcgcc
    16021 gaaatggaaa aacgctaccg cctgctttcc cacgccggtg tacgcaatct ggaaggcttc
    16081 aaccaaaaag tcgaagccgc aaaagcggca ggcaagcctt tgcttaatcc gttcagcctg
    16141 aaccccgacg agcccgagcc gctggaaaaa ctgccgttga ttgtggtcgt tatcgacgaa
    16201 cttgccgacc tgatgatgac cgaacgcaaa gccgtcgaac agcaaatcgc ccgtctcgcc
    16261 caaaaagcgc gcgccgccgg tatccatatg attgtcgcga cccaacgtcc cagtgtcgat
    16321 gtcgttaccg gcctgattaa agccaacatc ccgacgcgta tggcgtttac cgtgcaaagc
    16381 aaaatcgaca gccgtaccat cctcgaccaa atgggcgcgg acgaactgct caaatatggc
    16441 gattcgctgt tccttcagcc cggcagtgcc gaaccgaccc gcctgcaagg cgcgtttgtt
    16501 tcagacgacg aagtacatca agtcgtcaac tacgtcaaat cgcaagcccc agccgactat
    16561 attgaaggtc tgctcagcgg cgaggccgcg ctggaaactg ccaatatcgt taatccgaat
    16621 gcagacagcg acgaattgtt cgatcaggca gtcgcctatg ttttggaaag caaaaaaacc
    16681 tccatttcgt ctttgcagcg gcagttgcgt atcggctata accgcgcggc aaacctgatg
    16741 gaggcactgg aaaatgcggg tgtcgtttct cccaccgacc tcaacggcag ccgtaaaatt
    16801 ttggcgcaca aggaccattt gtagcccgta ttgcaaaatg ccgtctgaac ggcggaattg
    16861 gcgtttcaga cggcatatta tgtttcaggc gaaacattgt gatatacttg ccagctaaaa
    16921 tttccccttt gcggcaatgc ggttcaaata tcgtaccgtt gcgctgtttg cttccccatg
    16981 tagggaagaa agtttatcat tttatcaaca caacaaattt aagggcttat gatgagcgta
    17041 actgttgaaa ctttagaaaa tctggaacgc aaagtagtgt tgtccctgcc ttggtccgaa
    17101 atcaacgcag aaaccgataa aaaactgaaa caaactcaac gccgtgcaaa aatcgacggt
    17161 ttccgtccgg gtaaagcacc tttaaaaatg attgcccaaa tgtacggtgc gagcgcacaa
    17221 aacgacgtga tcaacgagct ggtgcaacgc cgcttctacg atgttgccgt tgcccaagag
    17281 ttgaaagtgg caggcttccc ccgttttgaa ggcgttgaag aacaagacga taaagagtct
    17341 ttcaaagttg ccgccatttt tgaagtgttc cccgaagtcg ttatcggcga tttgtctgca
    17401 caagaagttg aaaaagtaac cgcttccgtc ggtgatgccg aagtggacca aaccgtagaa
    17461 atcctgcgca aacaacgtac ccgcttcaac catgtcgaac gcgaagctca aaacggcgac
    17521 cgcgtcatca ttgactttga aggcaaaatc gacggcgaac cttttgcagg cggcgcatcc
    17581 aaaaactacg ccttcgtatt gggcgcaagt caaatgctgc ctgaatttga agccggcgta
    17641 gtcggcatga aggctggcga aagtaaagac gttaccgtca atttccctga agactaccac
    17701 ggtaaagacg ttgccggtaa aactgccatg ttcaccatta cgctgaacaa cgtttccgaa
    17761 gcgactctgc ctgaagtcga tgcagatttt gcaaaagcct tgggtattgc ggatggcgac
    17821 gttgccaaaa tgcgcgaaga agtgaagaaa aacgtaagcc gcgaagtgga acgccgcgta
    17881 aacgaacaaa ccaaagaatc cgtaatgaac gcgctgctca aagccgtaga gctgaaagca
    17941 cctgttgctt tggtaaatga agaagccgca cgcttggcaa acgaaatgaa acaaaatttt
    18001 gttaaccaag gtatggctga tgctgccaac ttggatctgc ctttggatat gtttaaagaa
    18061 caagccgaac gccgcgtgtc tttgggtctg attttggcca aactggttga tgaaaacaaa
    18121 ctggaaccga ctgaggagca aatcaaagcc gttgtcgcca acttcgcaga aagctacgaa
    18181 gatcctcaag aagtaatcga ctggtactac gcagagcctt cccgcctgca agccccgact
    18241 tctttggcga tagaaagcaa cgtcgttgat ttcgttttgg gtaaagccaa agtaaacgaa
    18301 aaagctttgt cttttgacga agtgatgggc gcgcaagcct gattatcctg aaaatgccgt
    18361 cttagggcag tttttgaaag caccgaagcc gcttttaaag cccgctttgg tgcttttaca
    18421 tcatgaaagg agacgaaatg tcttttgata actatcttgt ccctaccgtt atcgagcaga
    18481 gcggtcgcgg tgagcgtgca ttcgatatct attctcggct tttgaaagag cgcatcgtat
    18541 tcttggtcgg cccggtaacc gacgagtccg ccaatctggt ggttgcccaa ctgttgtttt
    18601 tggaaagtga gaatccggat aaggatattt tcttctatat taactcgcca ggcggttcgg
    18661 taacggccgg tatgtcgatt tacgacacca tgaattttat caagcccgat gtatcgactt
    18721 tgtgcttggg gcaggcagca agtatgggcg cgttcttatt gtcggcaggc gaaaaaggca
    18781 aacgtttcgc cctgcccaac agccggatta tgattcacca gcctttaatc agcggcggct
    18841 tgggcggtca ggcatccgat attgaaattc acgcacgcga actgttgaaa atcaaagaaa
    18901 aactcaaccg cctgatggcg aaacattgcg gccgcgattt ggcagatttg gagcgcgaca
    18961 ccgaccgtga taatttcatg tccgccgaag aggctaaaga atatggtctg attgatcaaa
    19021 tcttggagaa ccgtgcttct ttacagtttt aataagaaat ctgagagaaa caaatgccgt
    19081 ctgaaaacag ttcaggacgg catttgtctt atagggactg tatagggatg attttgttct
    19141 ggttgcgttt ggagccgttt tcaccatagt tggttttgac atattcctct acaatagcta
    19201 ggaattcagt tgcaatatta tcacctttca gtgtgacttt gcgctccccg tccacataaa
    19261 ccggcgcgac gggtgtttcc cccgtaccgg gcaggctgat gccgatgtcg gccaatttgc
    19321 tttctccggg cccattgaca acgcagccca ttacggcaac gttcagggat tcaaccccgg
    19381 gataaagggt acgccatata gacatttttt ggcgcaggta attttgaaca tcttgtgcca
    19441 gctcttgaaa tacggtactg gtggtacgcc cgcagccggg gcaggcggta accatcggcg
    19501 taaacgaacg caatcccata gtctgtaaaa tctcttgccc gacgacgacc tcctgagtac
    19561 gcgggctgcc aggttccgga gtcagtgaaa tgcggatggt gtcgccgatt ccttcttgaa
    19621 gcaagacgga taatgccgcc gttgatgcga caatgccttt gctgcccata ccggcttcgg
    19681 tcaaacccaa atgcagcgga taggcgcaac ggctgcccag ttcgcggtaa acctgaatca
    19741 aatcctgaac cgcgctgact ttgcacgaca ggatgatttt gtcttcgggc agtcccaata
    19801 gaacggcttt ttcggcagat tccaaagcgg agacaatcag tgcttccttc gtcacttctt
    19861 cgggcggttt cggcgcggaa aaagcgaggt tggcatccat catacgtttg gcgaggcttt
    19921 gatccaaaga accccagttt acgccgatgc ggacggcttt atcgttttca gcagcagtcc
    19981 gaatcataaa ggcaaatttt tcatcgcctt ttacgccttt gccgacattg ccgggattga
    20041 tgcggtattt ggacaatgct ttgccgcatt cgggaaattc cgccaacagg cgttcgccgt
    20101 tgaagtggaa atcgccgata agcggtgtgg catagcccat atcgtccaag cggcggcgga
    20161 tttcggcaac tttggacgcg gcttcggggc tgttgacggt aatacgcacc atttcggatc
    20221 cggcatcgct caattcctta atctgcaatg cggtggcttt tgcatcggca gtgtcggtgt
    20281 tggtcataga ttggataacg acgggtgctt ctgaaccgac ggtaatatga tcgatgcgga
    20341 cttgatgcgt cttgcggcgt tggagtgtgt tcatatggtt tgaatctgtt tattgaccgg
    20401 tgaggacggt ttgcaattct tccgagtagg ggaaattcgc ctgcaattgt gcttcatatt
    20461 cgtatgccgc ctgtgcgttg ccgagggctt tggcaatttt ccagcctagc agcaaatcat
    20521 cggcctgaag gacttctacc ctgctttggt attttttaaa gtagtaatcg gcatcgccca
    20581 actgcccggc cagcattttg gtgcgcgcca gttctttaaa tgcgggtggg aactgcggct
    20641 gggcggcgag ggaacgtttc aaataggctt ccgccaatcc gaattgcccc tgttttgcgc
    20701 tgcatatgcc tttattcagg ttggcaatat aaggggtcgg gtaggtgggg tcggccaggg
    20761 ctttgtcgaa atatgccata gattcggcag ggcggttgag cctgccgcac aggaaccagc
    20821 cgtagttgtt gttgatttcg gcactgtcgg gtttgatgga gagggcttgc cggaaacttt
    20881 cctgcgcctt gtcgttaact ttcaggtatt gatagatttc ggcacggacc agccaggcaa
    20941 gctcgttttt agggtctgat ttcaaggcgt cttcaatact tgccgtcgcc tgacggtagt
    21001 cctgaccgcg catatattcc attgccaact gggttttgat attggaaacc tgattggctt
    21061 tttctgcccg cgaggggcgg taggaagtgc tgcacgcgcc caaggcaagg acaagtaata
    21121 aagagattcg tttggatggc ttaaaaggca taattacccc tgttgtccga ttaaaatctg
    21181 ctgccatttt tgttggcggc gcgttttatc ctgaacctgc cccgccaact gtccgcaggc
    21241 ggcatcgatg tcgtcgccgc gcgtttttcg tacggtaacg acaaatcctg cctgctgcaa
    21301 aatatcgcgg aacacacgga tgttctcatt gctggagcgt tcgtatccgg agtttgggaa
    21361 gggattgaac ggaatcagat tgaacttgca gggaacatct gtgaccagtt cgatcagttc
    21421 gcgcgcatgt tgcgccttat cgtttattcc gtccaacatg acgtattcga aagtgatgaa
    21481 atccctgggt gctttgacca gatagcgttg gcatgcggcc atcaattctt tcaagggata
    21541 ttttttgttc aacggtacga tttggttgcg gacttcgtca ttggaagcgt ggagggaaac
    21601 cgccaaagcc accggcatga catcgcgcaa cctgtccatt tggggaacca tacccgaagt
    21661 ggaaacggtt acgcggcggc ggctcaaacc gtagccgtgg tcgtccagca tgatgcttaa
    21721 ggcggtaacg acattgtcga agttcgccat cggctcgccc atgcccatca tgacgacgtt
    21781 ggaaataacg cgctcgtttt tcggtgtaac gcccatcgct ttgtttgccc accacaattg
    21841 cccgatgatt tcggcagcag tcaaattgcg gttgaagccc tgccgaccgg tcgaacaaaa
    21901 tgtacattcc aaagcgcagc cgacttgtga ggaaatgcag agcgtgccgc gatccgattc
    21961 ggggatgaag acggtttcca ccccgttgcc cgtaccgaca tccaaaagcc attttcgagt
    22021 gccgtctgaa gatttttgag acatcatcag cttgggaatt tcgatgcctg cctgttcgtt
    22081 cagtttatgg cgcaacgatt ttgccaaatc ggtcatttcg tcaaaatttt gcgcgccgga
    22141 ttggtgcatc caacgcataa cctgtttggc acggaaaggt ttttcgccca tatcggcaaa
    22201 atgtcgggtc agcccttgga ggtcgtagtt gagcagattg gttttcatgt atttgtgttt
    22261 ttcttaaatt aaggcttcaa ataaaaaatt gcagggcagg caagtttgat ttgaagcctt
    22321 ttgcaacaga gttttcagac ggcatggata tattttatgc cgtctgaaga ggagttgtgc
    22381 ggtgtattgt atcaacgggg gcagatttcg gtttggctga aaaagtaggc aatttccaaa
    22441 gcggcatttt ccggagtgtc cgagccgtgt acggcattga tgctgaccga agtggcaaag
    22501 tccgcgcgta tcgtgccttc ggcggcttcg gaaggattag ttgcacccat cagttcgcgg
    22561 tttttcagaa cggcgttttc accctctaat acctgaatca taaccggacc gccggtcata
    22621 aattcaacca atccggcgta gaaggggcgg tctttatgaa ccgcataaaa ttcttgcgcc
    22681 tctttgaggg taagctgctt cattttggcg gcaacgattt tcagaccgtt ctcctcaaag
    22741 cggctgtata ttttgccgat aacgtttttg ccgacggcat cgggtttgat gatggagatg
    22801 gtacgttcaa tcgccatgct atatccttat ttttacttaa gaagaatcaa atcggtattc
    22861 tatcaaaaaa ataacttatc cgcctgaagc cttgggagat agggtcaggt gcggctgttc
    22921 caaataaact tcggactgca tttcgaccat ccggctggcg gtgcgggtaa attcttcggc
    22981 aaaatcgcct tctgtataga tatggttgac atctaccgcg ccggtggcac acagtttgac
    23041 acggaaatcg tagagtacgt caatcagcca agtcagccgc cgtgcctccg ccttttcttg
    23101 cggtgagagt tgttccaaac ctgaaataaa aaccatttca taatgttcgg ccaaatacag
    23161 gtagtcggac tgtgagcggg ggccgaagca cagtgcgcgg aaatcaaacc atatggcacg
    23221 gccggactcg gctttgtggg gaatctcccg accgtggatg gtgctgatgc cggggttcaa
    23281 atcggtaatg cctgtcattt ctttgaacag ttttgccagt tttgcctcat tttcttcatt
    23341 ggcaggcgta aagaaaatct cggcggggcg gagggtacgc agtcggtagt cttcaccgcc
    23401 gtcaacattt aagacggtca ggctggactc gatgagcgcg attgtgggaa gaaaactgct
    23461 ccggttttga ccttgcgggt agagttcgga aggcgcgtag tttgaagtcg ccaccaaaac
    23521 aacgccctcg ttaagcaggt tttccagcag acggcctaaa atcattgcat ccgcaatatc
    23581 gctgacatga aattcgtcaa aacacaatac gcgggtttct ttggcaatct cggcggcaac
    23641 ggatttcaac gggttgcttt cgcttttcag ggttttcagc cgccggtgga tttctgccat
    23701 aaaggcatga aagtggacgc ggcgtttgcg gcggtaaggg aggcagccga aaaaagcgtc
    23761 catcagaaag cttttgccgc gtccgacccc gccatagaaa taaagccctt tggggacttg
    23821 cggggaacgc aaactcctgc ctaaaaaacg gtttcttttg cgtttgaaca tcatcaattc
    23881 ggtccaaagc cgatcgaggt gttcgatggc ggcagcctgc gcgtcgtcgc ggatgaagtt
    23941 gggcagttgt gaggcagcct gataccaggt cagcgggctg tggttttcaa acggcggggc
    24001 tttaaaaagt tggtctctac tactcatatt taaccttttt atacttcttg cggctaagaa
    24061 agaaaatgct tactgtcggt attataaatg atttccttca aacttggcat tttccgtgga
    24121 aaagaataat gacgcgcaca agggggtatg agcaaaggcg gcgtttcaat atggtgcaga
    24181 tatgagctca aatgcctttt atagt
//
LOCUS       DAXHPG010000013        22502 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXHPG010000013 DAXHPG010000000
VERSION     DAXHPG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22502
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.013"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(156..995)
                     /gene="htpX"
                     /locus_tag="ACFM6F_000375"
     CDS             complement(156..995)
                     /gene="htpX"
                     /locus_tag="ACFM6F_000375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC6163860.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            1210..1857
                     /gene="adk"
                     /locus_tag="ACFM6F_000376"
     CDS             1210..1857
                     /gene="adk"
                     /locus_tag="ACFM6F_000376"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC6163861.1"
                     /translation="MKALFLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            2383..3123
                     /gene="pyrF"
                     /locus_tag="ACFM6F_000377"
     CDS             2383..3123
                     /gene="pyrF"
                     /locus_tag="ACFM6F_000377"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC6163862.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            3178..4149
                     /gene="hldA"
                     /locus_tag="ACFM6F_000378"
     CDS             3178..4149
                     /gene="hldA"
                     /locus_tag="ACFM6F_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC6163863.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            4186..5421
                     /locus_tag="ACFM6F_000379"
     CDS             4186..5421
                     /locus_tag="ACFM6F_000379"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6163864.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFSRVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            5423..6541
                     /locus_tag="ACFM6F_000380"
     CDS             5423..6541
                     /locus_tag="ACFM6F_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6163865.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEVLLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            6582..7586
                     /gene="rfaD"
                     /locus_tag="ACFM6F_000381"
     CDS             6582..7586
                     /gene="rfaD"
                     /locus_tag="ACFM6F_000381"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC6163866.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LNWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVTFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            7659..9203
                     /locus_tag="ACFM6F_000382"
     CDS             7659..9203
                     /locus_tag="ACFM6F_000382"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC6163867.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPSQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAIVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGSFFKKETNNNVLTEEHIADIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEIGETVAKIERLRREIDEV
                     IAEIEA"
     gene            9320..9403
                     /locus_tag="ACFM6F_000383"
                     /pseudo
     CDS             9320..9403
                     /locus_tag="ACFM6F_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible D domain protein"
     gene            9534..10701
                     /locus_tag="ACFM6F_000384"
                     /pseudo
     CDS             9534..10701
                     /locus_tag="ACFM6F_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            10688..10909
                     /locus_tag="ACFM6F_000385"
     CDS             10688..10909
                     /locus_tag="ACFM6F_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163868.1"
                     /translation="MPFGRVGFSPPKQQQAATTVKQRQAGRWAEAHPTASGNRPAFRH
                     SVWKPENMAADAQNKEKKPIEMIQTASVG"
     gene            11093..11205
                     /locus_tag="ACFM6F_000386"
                     /pseudo
     CDS             11093..11205
                     /locus_tag="ACFM6F_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            11236..14367
                     /locus_tag="ACFM6F_000387"
     CDS             11236..14367
                     /locus_tag="ACFM6F_000387"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC6163869.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENQLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSTDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFHDHIVDGKLI
                     MKGNALGSESTESVFGRQLHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKK
                     LSAAENRQALLHPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYE
                     AFKTQQAGSLHPLKVATIFSFAANEEQNAVGEIADETFEPEAMDSSAKEFLQAAINDY
                     NACFKTNFGTDSKAFQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNL
                     RYHGLMQAFSRTNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEE
                     YMNGYTDSQTGEARRGYLDVAKELHERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQ
                     NYDEFAALRELQSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYN
                     DIRDWLRRQKAGEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLIFEHHKKIKGKAE
                     LVEEIRRIIRASIGHRAKESLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRNEAAE
                     LIAAEGLNETAAKRYLISSLKRGYASENGTELTETMPKMSPLNPQYLTKKQSVFQKIA
                     AFVEKFAGIGTDI"
     gene            complement(14407..16698)
                     /gene="clpA"
                     /locus_tag="ACFM6F_000388"
     CDS             complement(14407..16698)
                     /gene="clpA"
                     /locus_tag="ACFM6F_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC6163870.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVHNV
                     LKLCGADLKVVSEQLAASVAENTPLIPDHLLDTVETQPTLGFQRVIQRAMVHTQSAGK
                     GLVEPLDVLVALMSETDSHAVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKK
                     VEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(16700..17002)
                     /gene="clpS"
                     /locus_tag="ACFM6F_000389"
     CDS             complement(16700..17002)
                     /gene="clpS"
                     /locus_tag="ACFM6F_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC6163871.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            17245..17448
                     /locus_tag="ACFM6F_000390"
     CDS             17245..17448
                     /locus_tag="ACFM6F_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC6163872.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            complement(17745..19076)
                     /gene="pmbA"
                     /locus_tag="ACFM6F_000391"
     CDS             complement(17745..19076)
                     /gene="pmbA"
                     /locus_tag="ACFM6F_000391"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC6163873.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRASTADFSEKALQDTVKAAIDIARYTAEDDCA
                     GLADASLMAQHIGDPDLYHEWDLDTEAAVELAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHQQGTRHSISCSVVAADENGMQRDYWYDSSCRHQDMDSPTQIGQT
                     AAERTLRRLNSRSIPTGSYPVLFDATVSGGLIGHLVGALSGGSLYRQSSFLIDSIGKQ
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            19231..19743
                     /gene="yjgA"
                     /locus_tag="ACFM6F_000392"
     CDS             19231..19743
                     /gene="yjgA"
                     /locus_tag="ACFM6F_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC6163874.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAVHNAFLQRVE
                     QARVRLLADDGALTQFMSDFPNADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MESQGGTGEA"
     gene            19790..20509
                     /locus_tag="ACFM6F_000393"
     CDS             19790..20509
                     /locus_tag="ACFM6F_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163875.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDKPADIAKLKQLGFEAVELDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFANILYTPPPFLILDA
                     AGAVLALPAAALGAVVDAARK"
     gene            20578..22278
                     /gene="recJ"
                     /locus_tag="ACFM6F_000394"
     CDS             20578..22278
                     /gene="recJ"
                     /locus_tag="ACFM6F_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC6163876.1"
                     /translation="MSAKIQTRSVNTDVFNHLLTAGADPLIAQLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVGRKEKILIVADYDADGATACSVGMSGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDTEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPARDITLEQAQNLAKHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
BASE COUNT         5952 a         5459 c         5815 g         5276 t
ORIGIN      
        1 tgtccggaca cggtttatca gggtatatca atgcggcgta tccggtgcgg aaatggatac
       61 ggttggtgtt ggtatggaaa cctgatgttt tcagacagca tatacaaaaa accgtactgc
      121 ttgcgcgtac ggtttttttg ttgtttcaaa tcggctcaga gcgatttgag gcgggcgatt
      181 cggttgtcca gcgaagggtg ggtgctgagc agggagtcgc gcgtatctcc ggcgatgccc
      241 attgcgttca tttcttcggg caaatcgacc gggttgcctt taagcctttg cagggcggaa
      301 atcattttcg gtgcgccgac cagttttgcc gcgcccgcgt cggcgcggta ttcgcgttgt
      361 cggctgaacc acatgacaat taagctggca aggaagccga ataggatttg gaataccatg
      421 ctgaccagga aataagttcc ctgcgactgg ctgccgtcgt tgtttcgggc aatcaggttg
      481 gcaataatgc gcgacaggaa cacgacgaag gtattgacca cgccttgaat cagcgtcagc
      541 gtaaccatat cgccgttgcc gacgtgtgcc atttcgtgcg ccaatacggc ttccacttcg
      601 tcgcgcgtca tatggtcgag caaaccggtg ctgacggcga tcagggagct gtttctcgat
      661 gcgcccgtgg caaaggcatt gggttcgggg gagtggtaga tggcgacttc gggcgttttc
      721 aggttccatt gccgcgcttg ggcttcgaca gtgttcaaaa gccaggcttc ttcttcggtg
      781 cgcggcgtgt cgataacttc cgcgccgacc gattgtttgg cgatgaattt ggacatcagc
      841 agcgaaataa tcgaaccagt gaagccgacg acggcggaat acgccaacag gctgcccgtg
      901 ccgccccggc tgttgatgcc caaaaccgcc aaaacaatgt tgattacgac caaaacagcg
      961 atattggtag ccaaaaacag aaaaattcgt ttcacggatg ttcctttttg gtagggtgtg
     1021 atgttttgaa attttgggga ttgtcccaaa aagttgccgg cttgtgaata tcagactcgg
     1081 caaaggtatg caaaacattt gcttgcaaat ggcagtttgt gcagttggtt tttgaactat
     1141 tgtgccaagc cgtgtagaat cgtaaaccat ctgtttgatt ccaataaaca catttcaaag
     1201 gatcacttca tgaaagcatt atttttaggc gcgccgggcg cgggcaaagg cactcaggcg
     1261 caattcatca ccgccgcgtt cggcattccg caaatctcta ccggcgacat gctccgcgcc
     1321 gcgattaagg caggcacgcc cttgggtttg gaagcgaaaa aaatcattga tgaaggcggc
     1381 ttggtgcgcg acgacatcat tatcggcatg gtcaaagaac gcatcgcgca agacgactgc
     1441 aaaaacggtt tcctgtttga cggtttcccg cgcacattgg cacaagccga agcgatggtt
     1501 gaagcaggcg tggatttgga tgcagtcgtt gaaatcgatg tgcctgacag cgtgattgtc
     1561 gaccgcatga gcggccgccg cgtgcatttg gcttccggcc gtacttacca cgttacctac
     1621 aacccgccca aagttgaagg caaagacgac gtaaccggcg aagatttgat tcagcgcgac
     1681 gacgacaaag aagaaaccgt gaaaaaacgc cttgccgttt accacgagca aaccgaagtt
     1741 ttggtcgatt tttacagcaa actggaaggc gaacacgcgc ctaaatacat caaagttgac
     1801 ggcactcagc cggtagaagc cgtgaaagcc gaagtattgg gcgcattggg caaataaatc
     1861 gaaaaggtcg tacccacggg caggcttcgc actctgaaaa cagaaaatca ggttttcaga
     1921 cgacctgttt ttgataaaca gcgtgttgca accgaaaaat aatcatttgg cgtcattccc
     1981 gcgcaggcgg gaatccattt ctgaatttgg gcaatcgctg tttaaatctg atgaactgag
     2041 ttttatcaat ggattcccgc ctacgcggga atgacggctg atgtaccggt tcaaatttat
     2101 ccgaaacagt ttgtcggagg cttgagtccg cgtaggtcgg acatcaatgc ccgacctacg
     2161 gtttgaattt acgttcttat agtggattaa caaaaatcag gacaaggcga cgaagccgca
     2221 ggcagtacaa acagtacgga accgattcac tttgtgcttc agcaccttag agaagcgttc
     2281 tctttgaact aaggcgagac aacgccgtac cggtttaaag ttaatccact atactgcgaa
     2341 aaagacgata aaggtcgtct gaaaacccga aacgaaaaca ccatgaatcc tttaatctcc
     2401 gacttccaaa ctccgcaaca acgcaccccc gttatcgtcg cccttgattt ttccaacgaa
     2461 aaagacacgc tcggattcgt ccgcaacctt gacccgacat tgtgtcaaat caaaatcggc
     2521 aaagagctgt ttaccgcgac gggacgcaat ttggcagaaa gcttaatcaa tcaaggtttc
     2581 aaacttttcc tcgatttgaa ataccacgat attccccaca ccgtcgcgca agcctgcaaa
     2641 gtcgctgccg atatgggcgt ttggatggtc gatatgcacg catcgggcgg ccgccgtatg
     2701 atggaagccg cagcagaagc cgttgccgga tacggcacga agccgctctt aatcggcgta
     2761 accgtgttga ccagcatgga acaaagcgat ttggcggaaa tcggtttgaa caccgcccct
     2821 gaagaacaag tcatccgctt ggcgaaactg gcgcaaagtt cgggcttgga cggcgtggtc
     2881 tgttccgccc aagaagccgc accgctgcgc cgcgagttgg gacaagattt tgtcttggtc
     2941 acgcccggca tccgcttgga cgttgccggc aataatgatg accagcgccg catcatgaca
     3001 ccggccgaag ccttggctgc cgcttcgact tatttggtaa tgggtcgtcc tgtaacccaa
     3061 gctgccgatc cggtagccgt attgcgcgaa gtgaaccgcg tggcaaacct tgaagcaaac
     3121 tgattttcag acggccttac aggctgaggc cgtctgaaaa aatacaacgg aggcaatatg
     3181 tccgccaagt tccaacaaga aaccctcaaa tcccgtttcg cgcaagccaa agtcctggtt
     3241 gtcggcgacg tgatgctcga ccgctattgg ttcggcgatg tgtcccgtat ttcgcccgaa
     3301 gcccccgtgc cggtggcgaa aatcggacga atcgaccaac gcgcgggcgg agcggcaaat
     3361 gtcgcgcgca acatcgcttc gttgggcggc agggcagggc tgttgtccgt aaccggcaac
     3421 gacgaagccg ccgacgcgct cgatgcgctg atggtgcagg acggcgtcgc ctcctatctg
     3481 atgcgcgaca aacaaatcgc caccaccgtc aaactgcgcg tcgtcgcccg caaccagcag
     3541 cttatccgtc ttgattttga agaacatccc aactgcgaag tgttggaaca aatcaagcag
     3601 aaataccgcg aaatcttgcc cgaatacgac gcaatcattt tttcagacta cggcaaaggc
     3661 ggcctgtcgc atatctccga tatgatcgat tgggcgaaac acgccggcaa aaccgtctta
     3721 atcgacccca aaggcgacga ttacgaaaaa tatgtcggtg caactctgat tacgcctaac
     3781 cgcgccgaat tgaaagaagt ggtcggcagt tggaaaaacg aaagcgagct gaccgaaaaa
     3841 gcgcaaaacc tgcgccgcca cctcgacctg accgccgttt tactgacccg aagcgaagaa
     3901 ggcatgacct tgttcagcga aggcgaaccg atttaccagc ccacccgcgc ccaagaagtt
     3961 tacgacgtat ccggtgcggg cgacaccgtc attgccggaa tgggcttggg tttggcggca
     4021 ggctgcacca tgcccgaagc catgtacctt gccaatactg cggccggggt tgtcgtggcg
     4081 aaactcggta cggcggtttg ctcgtttgcc gaattgatca aggcattgtc agggcaatca
     4141 acaatgtagt tttcatattg ataagataaa cagaacgata taagtatgac tatttcgaca
     4201 atggcacaga cacacgatac tcgattacaa aaaactttgc tctttcccag tcaacaaggt
     4261 tggaaatcta aaacttttct taaacctgat tcacatatta gattagcaac cgtattcagc
     4321 gggattggtg cggttgaaca ggcattccac cgattaaatt taaaccatac cattgttttt
     4381 gcaggagata ttgatccata cgttaaaaaa agttatcttg gaaactataa attaaatgaa
     4441 gatttttggc ataacgacat tactcaattt gatgcgagaa agtttagaaa tcaagttgat
     4501 attttagttg gaggcagtcc ttgccaagca ttttccatgg ttggcaaacg tgcaggatta
     4561 gaagatacac gaggaacatt attctatgaa ttttctcgcg ttgtggatga agtccaacca
     4621 aagatattta tttatgaaaa tgtaaagggc ttgctcaatc atgataatgg aaaaacttgg
     4681 aaagttgtaa aaagtgtttt ttattcactt ggttatgact tatatttcca aataatgaat
     4741 agtaaggatt atgggattcc tcaacatcgt gagcgtattt ttgttgttgg ctttcatacc
     4801 cctcctataa atggttttca gtttcctgaa aagattgaat tagaacatac tatgcaagat
     4861 tttttggagg actatactga tagcaaatat tttttacgtg aaaagggtgt gaaatttgtt
     4921 accagttcta aaaatagaca aaaacgttat acacagatta atggagaaat tgccttatgc
     4981 caaaaagcaa atcaacaatt taattggcat ggtgatttta tttttcaggc agcccgcgaa
     5041 tctgaatttg atgactttat ttttgatgta aataacgttg aggaaaaata ttatctttct
     5101 gaaaaaatca aaaattatgt tttggcagga ggaacaaaaa attttaaaac cagtacggaa
     5161 actgatttgc ctgtagctcg cccattattg caaactatgc ataaaatgca tcgtgccggg
     5221 gttgataatt atgttactca taatcgtgga cgtattcgta aattaacacc tagggaatgt
     5281 ttgcggctaa tgggttttag agatgatttt aaaattttag ttagtgatac tcaaatgtat
     5341 cgccaagcgg gaaacagtat tgttgttgat gtattaatcg ctatattaaa acaaatggat
     5401 attacgcttt atggagagta aaatgccgga ttttcaaaga attactattg aaaacataga
     5461 gtattttatt gtcgatagta ttcaaaattt acgtgcggaa gatagtttta ttcacaggaa
     5521 taacaagtta tctgtttttg gtggtaatgg ggaagcacgc aaatatgtag gtagttatat
     5581 tgatgatagt ggtcgaaaac taagtacatt ttttgagtat gaaaattggg ggattactga
     5641 gacaaaaaat ggaaggaggg tagaatatcc tctcattcag aaaaattgtt tttttaataa
     5701 gaaaaattta cagcgttatt tggttgatgc aaaagttgaa tatcacaaac aagaacagaa
     5761 atatcatcat gatatttcaa ggttttatga tgattacgtt ttaagtatta ataatctgcc
     5821 tacagaaaat attttctttt caatccatga tgtatcagac catttggaaa attctaaagg
     5881 ttaccgtagg ggttatatcc gttcggagga tgatatatgg tcaatttggc gtaagattgt
     5941 attgcccaaa attagttatt tatcaattct taaactattg cctgtcaaag atatagaaga
     6001 ttcagaacca ttattttatt tccgaatatt tttggattat caatttcgct ctatcgtgca
     6061 cccgcaactc ttatcaagag aaaaattaga aataccggct tcagaattta atcaattcgt
     6121 agagcaagaa atcattaaac agaaaagtag aaaagggcag cagaaatatc gcaaggatgt
     6181 tataaatcat atgccgcaat gtccatttac attaattaca gatgaggttt tgttaagggc
     6241 tagtcatatt aaaccttata tggtttgtat tactgagaaa aatgagaaag aggcattaga
     6301 ttatttaaat gggttagctt taacgccgac ttatgattgg ttgttcgatc aaggttatat
     6361 tactttcttg gatgatggtc gtttgatttg cggtactcga ctaagccgtt acacatggga
     6421 aaaacttaat atcaatccaa atgcaaaaaa taagatgaga atatatcccg aaggaagaga
     6481 aaaatatttg gaatatcatc gcaaatttgt gtttcaggac aatatcgatg atttcttgta
     6541 actaagttta ttaattttac tcaaaacaaa ggaaaccgaa tatgaccatc atcgtaacag
     6601 gcgcggccgg ctttatcggc agcaacatcg tcaaagcact taatcaacgc ggtattaccg
     6661 acatcgtcgc cgtcgataat ttgagcaaag gcgaaaaatt caaaaacctt gccgagtgcg
     6721 aaatcgccca ttatctcgac aaacacgaat tcatccgcca agtgagggaa cacattttac
     6781 cttatcaaaa catcgaagcc gttttccatc aaggcgcgtg ttccgatacg atgaaccacg
     6841 acggtttgta tatgatggaa aacaactacc agtacacgct ggatttgttg aactggtgtc
     6901 aggacgaacg cattcccttc ctttatgcct ccagtgcggc ggtttacggc aaaggcgaaa
     6961 tcttccgcga agagcgcgaa ctcgaaaaac cgcttaatgt gtacggctac tccaaattcc
     7021 tgttcgacca agtattgcgc cgccgcatga aagaaggtct caccgcccaa gtcgtcggct
     7081 tccgctactt caacgtttac ggacaacacg aacaacacaa aggccgcatg gcatccgtca
     7141 ccttccacca tttccaccaa taccgcgaac acggttacgt caacctgttc ggcagcaatg
     7201 acggttacgg caacggcgaa caaacccgcg actttgtcag cgtcgaagac gttgccaaag
     7261 tcaacctcta cttcttcgac catcccgaac tttccggcat ctacaacctc ggtaccggcc
     7321 gcagccaaca gtttaacgaa ctcgccgccg ccaccgtcaa cgcctgccgc gccgccgaag
     7381 gcaagcctga aatgagcttg aaagagctgg tggaagaaga acttatccgc tacatcccct
     7441 tccccgacgc actcaaaggc aaataccaga gcttcaccca agccgacatc accaaattgc
     7501 gtgaggcagg ttatacggaa gaatttttcg atgtcaaatc aggcgtcgag cgctacgtca
     7561 aatggatgct ggaaaatttg gcttaatttg aatgcccgta aaaaatcgtc tgaaaatatc
     7621 aggcgatttt gatttgttta acttttatat ggatttcgat gatgaccgaa atgcaacaac
     7681 gcgcccaact gcaccgccaa atttggaaaa ttgccgacga agtacgcggc gcggtggatg
     7741 gctgggactt taaacaatac gttctcggca cacttttcta ccgctttatc agcgaaaact
     7801 tcaccgacta tatgcaggca ggcgacagca gtattgatta cgccgctatg ccggacagca
     7861 tcatcacgcc cgaaatcaaa gacgatgccg tcaaagttaa aggctatttc atctacccca
     7921 gccagctttt ttgcaatatt gccgccgaag cccatcaaaa cgaagagctc aacaccaagc
     7981 tgaaagaaat ctttaccgcg attgaaagct ccgcctccgg ctatccgtcc gaacaagaca
     8041 ttaaaggcct gtttgacgac ttcgatacca ccagcagccg gctcggcagc acagttgccg
     8101 acaagaacaa acgccttgcc gccgtcctca aaggcgtggc ggaacttgat ttcggcagtt
     8161 ttgaagacca ccacatcgac cttttcggcg atgcctacga atacctgatt tccaactacg
     8221 ccgccaacgc aggcaaatcc ggcggcgaat ttttcacccc gcaaagcgta tccaagctga
     8281 ttgcgcggct ggcggtgcac gggcaggaga aagtaaacaa aatctacgac ccagcttgcg
     8341 gctcgggcag cctgctcttg caggcgaaaa aacagtttga cgagcacatc atcgaagaag
     8401 gcttcttcgg gcaggaaatc aaccacacca cctacaacct cgcccgcatg aatatgtttc
     8461 tgcacaacgt caattacaac aaattccaca tcgaattggg cgacacactg actaatccca
     8521 agctcaaaga cagcaaaccc tttgatgcca tcgtttccaa tccgccttat tccatcaact
     8581 ggataggcag cgacgacccc accttaatca acgacgaccg ctttgccccc gcaggcgtac
     8641 tcgcaccgaa atccaaagcc gattttgcct tcatcctgca cgcactgaac tacctttccg
     8701 gcagaggccg cgccgccatc gtctctttcc ccggtatttt ctatcgcggc ggcgcagagc
     8761 agaaaatccg ccaatatctg gtggagggca actacgtgga aaccgtgatt gcccttgcgc
     8821 ccaatctctt ttacggcacc ggcatcgccg tcaatatcct ggttttgtcc aaacacaaag
     8881 acaataccga catccaattt atcgacgcag gcagcttctt taaaaaagaa accaacaaca
     8941 acgtcttaac cgaagaacac attgccgaca tcgtcaaact cttcgccgac aaagccgatg
     9001 tgccgcatat cgcccaaaac gctgcccagc aaaccgtcaa agacaacggc tacaacctcg
     9061 ccgtcagcag ctatgttgaa cccgaagaca cacgcgaaat tatcgacatc aaacagctta
     9121 acgccgaaat cggcgaaacc gtcgccaaaa tcgaacggct gcggcgcgaa attgacgaag
     9181 tgattgcaga gattgaggcc taggcagttg ttgcatattt tgcaacaact gaatgggaaa
     9241 gaggccgtct gaaaatacgg gcggtaacct gtaagtaatc tttacaagtt caaatcaaga
     9301 aattttcggg gaaaaataca tgacaacaga aaacaacgct tttgaaaacg ccaaacacat
     9361 cgacgaaaca ggcaatgaat attggtcggt gtgcaccttg cagacaaagt agggtgggct
     9421 tcagcccccc gaaacaacgg caaaacagca ggcggattgt tgggctggag cccaccctac
     9481 ggcggataac cgccccacat cccgccgcag cgggaaagaa acggaaaaca accatggata
     9541 tacaaagtaa agcgaaaaaa ttgattgaga tgattcagac ggcaccggtg gagtggaagc
     9601 cgttggggga agtggcaagt ataggaaagg gaaagcagct taataaatca ctacttagag
     9661 aaaaagataa gtatcccgta atcaatggcg gtattaatcc atcaggttat tggcatgaat
     9721 acaataacga agctggcacg attgttatta gccaaggagg agcaagtgcg gggtttgtta
     9781 attggatgga tatccctttt tttgcaggag ctcacgcata tactattaaa gcgaacgaag
     9841 acaaactatt gcaacggttt gcatattatt tttaaaaatg aagcaaatta cattcatgca
     9901 atgtcaatac ggagcaggta taccagcact tgctaaaagt gaattgcaaa acttacttat
     9961 tcccatcccg cccctagaaa cccaacaaaa aattgtaaaa atacttgaca aattcaccga
    10021 gctggaagcg gaattagccc tgcgcaaacg ccaataccgg tattaccgcg actttctttt
    10081 agattttgac aatcaaatcg gggggggata gctgatggct atcaatgccg tctgaaagat
    10141 gtggtttgga agacgttggg ggagattgcc gaatattcaa aaaaccgtat ttgttcggat
    10201 aaactgaacg aacataatta cgtgggcgtg gataatctct tacaaaatag agaaggtaaa
    10261 aagttatccg gttatgttcc aagtgaggga aaaatgacag aatatattgc caatgacatc
    10321 ttgattggaa atattcgtcc gtatttgaaa aaaatctggc aggcagactg cacgggcgga
    10381 acaaacggtg atgttttggt tatccgggta acagatgaaa aggttaatcc aaaatatctg
    10441 tatcaggtat tggccgatga taaatttttt gcctttaata tgaagcacgc caaaggtgca
    10501 aaaatgccgc gtggcagcaa agcagcgatt atgcaatata aaatccccat cccaccactc
    10561 cccgaacagg aaaaaatcgt cgccatccta gacaaattcg acaccctgac ccactccgtc
    10621 agcgaaggcc tgccgtacga aatcgccctg cgccgcaaac aatacgaata ttaccgcgaa
    10681 cagctgcttg cctttcggta gggtgggctt cagcccaccg aaacagcagc aagctgcaac
    10741 aacagtaaaa caacggcagg cggggcggtg ggctgaagcc caccctacgg cgagcggcaa
    10801 ccgccccgca ttccgccaca gcgtgtggaa accggaaaat atggcggcgg atgctcaaaa
    10861 caaagagaaa aaaccgattg agatgattca gacggcatcg gtggggtaga agtcgcagac
    10921 aaagtagggt gggcttcagc ccaccgaaac agcagcaagc tgcaacaaca gtaaaacaac
    10981 ggcaggcggg gcggtgggct gaagcccatc ctacgatgga cacaaccgac ccgcattccg
    11041 ccgcagccaa ccaaagcagc ctgcacattt tcaggcagcc caatccgaac atcaaaccgt
    11101 ttatagtgaa ttaaatttaa atcaggacaa ggcggcgagc cgcagacagt acaaatagta
    11161 cggcaaggcg agccaacgca gtaccggttt aaatttaatt cactataaaa cccgacaccg
    11221 caaaggacga cccatatgaa cctcgaaact aaacccatcg ccgaaacgcc gaatttcatc
    11281 gtgctcgacc aatatgaaaa aatcgaacag tcgggcagct accaatcgga aaaccagttg
    11341 gaagcggagt taatcgccga tttgcagaat cagggttacg aataccgcaa ggatttgaac
    11401 agccaaagca ggctgctgga aaacctgcgc gcgcagttgc agcggctgaa cgatgtggcg
    11461 ttttcagacg gcgaatgggc gcggtttttg acggaatatc tggacaggcc gtctgaaaac
    11521 attaccgata aaacccgaaa aatccacgac gaccatattt acgatttcgc ttttgatgac
    11581 ggccgtctga aaaatattta tctgctggac aaaaaaaacc ttgcccgcaa ccatgtgcag
    11641 cttatcaacc agtttgagca gacgggcacg catgcaaacc gctatgacgt taccgtgttg
    11701 gtaaacggct tgccgctggt gcagattgaa ttgaaaaagc gcggcgtggc ggtgcgcgag
    11761 gcattcaatc aggtgcaccg ttacagcaaa gagagcttca acagcgaaaa ttcgctgttc
    11821 aaattcctgc aaatcttcgt gatttccaac ggcacggata cgcgctattt cgccaacacc
    11881 accaagcgcg acaaaaacag cttcgatttc acgatgaatt gggcgcggtc ggacaatcat
    11941 ccgattaagg atttgaaaga ctttactgcc acgttcctgc agaaaagcgt attgctgggc
    12001 gttttgctgc attacagcgt gttcgatgcg aatgatacgc tgctgattat gcggccgtac
    12061 cagattgccg ccgccgaacg cattttgtgg aaaatcaaca gttcggcgca ggcgaagaat
    12121 tggagcgggc cggaaagcgg cggctatgtc tggcacacca cgggcagcgg caaaacgctg
    12181 accagcttca aggcggcgcg tctggcgacg gaatcggcgt ttatcgacaa ggttttcttc
    12241 gtggtggaca ggaaggattt ggactatcag acgatgaagg aataccaacg tttttcgacc
    12301 gacagcgtga acggttcgga aagcacggcg ggcttgaaac gcaatttgga aaaagacgac
    12361 aacaaaatca tcgttaccac catccagaag ctgaacaacc tgatgaagag tgaagataat
    12421 ctgccggttt atcaccagca agttgtcttt attttcgacg aatgccaccg ttcgcaattc
    12481 ggcgaagcgc aaaaaaacct gaaaaagaaa tttaaaaaat tctgccagtt cggttttacc
    12541 ggtacgccga tttttcatga ccatatcgtt gatggcaaac tgattatgaa aggcaatgcg
    12601 cttggttcgg agagtacgga aagcgtattt gggagacagc tgcattctta tgtgattacc
    12661 gatgccatcc gcgatgaaaa agtattgaaa ttcaaagtgg attacaacga tgtgcgcccg
    12721 cagttcaaag ccgtggaagc ggaacaggac gagaagaaac tgagtgccgc cgaaaaccgc
    12781 caagccctgc tgcatcctga acgcatccgc gaaatcacgc aatatatcct gaaccagttc
    12841 aggcagaaaa cacaccgctt gaatgcgggc ggcaaaggct ttaacgccat gtttgccgtc
    12901 agcagcgtgg atgcggcgaa gtgctattac gaagcgttca aaacacaaca ggcaggcagc
    12961 ctgcacccgc tgaaagtggc caccattttt tcctttgccg ccaacgaaga gcaaaacgcc
    13021 gtcggtgaaa ttgccgatga aacctttgaa ccggaagcga tggacagcag cgcaaaagaa
    13081 tttttgcagg ctgccatcaa cgattacaac gcctgtttca aaaccaattt cggcacggac
    13141 agcaaagcct ttcagaacta ctaccgcgat ttggcaaaac gggtaaaaaa tcaggaagtg
    13201 gatttgctga ttgtggtcgg catgtttttg acgggttttg acgcgccgac gctgaacacg
    13261 ctgtttgtcg ataaaaacct gcgctatcac ggcctgatgc aggcgttttc gcgcaccaac
    13321 cgcatttacg atgccaccaa aaccttcggc aacattgtct gcttccgcga tttggagcag
    13381 gcaaccattg atgcgattac cttgtttggc gacaaaaaca ccaaaaatgt agtgctggaa
    13441 aaaagttacg aagaatacat gaacggctat accgacagcc agaccggcga agcgcgacgc
    13501 ggctatttgg atgtggcaaa agaattgcac gagcgtttcc ccgatcccga caaaatcgaa
    13561 acggaaaaag acaaaaaaga ttttgccaaa ctcttcggcg aatacctgcg ggcggaaaac
    13621 gtattgcaga actacgatga atttgccgcg ctgcgcgagt tgcagagtgt ggacgcggcg
    13681 gacgaagatg cgatgaaggc gtttcaagaa aaatactacc tgagcgatga agacgtgcag
    13741 gaaatgcggc aagtgccgat gccgtctgaa agggcggtgc aggactaccg ttccgcctac
    13801 aacgacatcc gcgattggtt gcgccgccaa aaagcaggcg aacagaaaga acagtcaaaa
    13861 atcgactggg acgatgtggt ttttgaggtg gatttgctca aatcgcagga aatcaatctg
    13921 gattacatct tgcaactgat tttcgagcat cacaaaaaaa tcaaaggcaa agcggagctg
    13981 gtggaagaaa tccgccgtat catccgcgcc agtatcggac accgcgccaa agagagcctg
    14041 attgtggatt tcatcaacga tacggatttg gataaagtcc ccgatgttcc cgccatactg
    14101 gaaaccttct acacctacgc tcaagaggtg atgaggaatg aagccgcaga gctgattgcc
    14161 gccgaaggtt tgaacgaaac cgccgccaaa cgctatttaa tcagctcgct caaacgcggc
    14221 tatgccagcg aaaacggtac ggaactgacc gaaaccatgc cgaaaatgag tccgctcaac
    14281 ccgcaatatc tgacgaagaa acaaagtgtt tttcaaaaga ttgcggcgtt tgtggagaag
    14341 tttgccggaa tagggacaga tatttgacaa aatgccgtct gaacctttca gacggcattt
    14401 ttgattttat gcggaggcgg tttttatttt gaccttgctt ttcttaaact tcaacacggc
    14461 ttcttctttt gccgcatccc agtctatccg cacgaagccg ccgtcggata gtttgccgaa
    14521 caggagttcg tcggcgagcg gtttgcggat tttttcctga atcaggcggt gcatcgggcg
    14581 cgcgcccatt tgcgggtcga aacctttttc cgccagatat ttgtgcaatg ccgacgtgaa
    14641 ttcggcttcg acttttttgt cgaggagctg gtgttcgagc tggagcagga atttgtccac
    14701 gactttgacg atgatgggtt cggacaaggg tgcaaacggg ataatcgcgt ccaagcggtt
    14761 gcggaactcg ggcgtgaaga gcttgttgat tgcctgcatt tcgtcgccgc gttcgcgctt
    14821 ggcggtaaag ccgaggctgg ggcggctgag gctttccgcg cctgcgttgg tggtcataat
    14881 caggatgacg ttgcggaaat cggcactctt gccgttgttg tcggtcagtt tgcctgcgtc
    14941 catgacttgc aggaggacgt tgaaaatgtc ggggtgggct ttttcgattt cgtccaagag
    15001 caatacgcaa tgcggctgtt tgttgacggc ttcggtcaaa aggccgcctt gttcaaagcc
    15061 gacgtagccc ggtggcgcgc cgatgaggcg cgatacggca tggcgttcca tgtattcgga
    15121 catatcaaag cgttgcagcg gtacgcccag cgagtaggca agctgtttgg cggcttcggt
    15181 tttgcccacg ccggtcgggc cggagaagag gaagctgcct atcggtttgt cgggcagggc
    15241 aaggccggaa cgcgacattt tgacggcggc aaccagcgcg tcgatggcgt tttcttgacc
    15301 gtaaaccatg tttttcaaat cgcgaccgag gaattgcagc acttgtttgt cgtcgtgcga
    15361 cacggttttt tcgggaatcc gcgcaacttt ggcgatgacg gtttcaattt gtgctttgcc
    15421 gatgactttt ttctgtttgg atttgggcag aatccgttgc gccgcgcctg cttcgtccat
    15481 cacgtcgatg gctttgtcgg gcaggaaacg ctcgttgatg tagcgtgcgg agagttcggc
    15541 ggcggcttcg agtgcgcctt gcgtatagcg gacttggtgg aaggcttcaa acatcggctt
    15601 caagccgcgc aggatttgta cggtttcgga aacggtgggt tcgaccacgt cgattttttg
    15661 gaagcggcgg cttaaggcgt ggtctttgtc gaaaatggtg cggtattcgt cgtaagtggt
    15721 cgcgccgatg cagcgcagcg aaccttttgc cagcgcgggt ttgagcaggt tggacgcgtc
    15781 catggtgccg ccgctggtgc tgcctgcgcc gatgatggtg tggatttcgt cgataaacaa
    15841 aatggcgtgc gggatttttt cgagctgttt caagacggat ttgacccgcg cttcaaagtc
    15901 gccgcggtat ttcgtgcccg ccaacagcga gcccatatcc agcgcgtaca cttcggcatc
    15961 tttgagcgcg tctggaatgc cgccgttgac gatttgatgt gccaaacctt ccgccagcgc
    16021 ggttttgccc acgcccgctt cgccgaccaa aagcggattg tttttgcggc ggcggcatag
    16081 gatttgcacc agccgttcca tttcgtgttt gcgaccaatc aaagggtcga tacggccggc
    16141 tttgacttcg gcgttgaggt tgacggtgta cgccgataaa gggtttttgc ccggtttggt
    16201 gcggttttca ttatcgtcgt ccatgccgtc tgaagaatag ttgccatcgt cttcatcttc
    16261 atcgggagag ccgtgggcaa tacagcgcaa aacttcaaaa cgcgtaaccg attgcagctt
    16321 gaggaaatag acggcgtggc tatcggtttc gctcatcagc gcgaccaaaa cgtccaaagg
    16381 ctcgaccaag cctttgcctg cggattgggt atgcaccatc gcccgttgga tgacgcgttg
    16441 gaagccgagc gtgggctggg tttcgaccgt gtctaaaagg tggtcgggaa tcaggggggt
    16501 gttttcggca acgctggcgg cgagttgttc ggacaccact ttcaaatccg cgccgcagag
    16561 cttgaggacg ttgtggacgg aggcatcttc ttcgatgagt accaaaagca gatgctcgag
    16621 gctgataaat tcataatgag ccttacgcgc ctcgcggtaa agctgctgca aaatctgttc
    16681 caattcgggt gcaagcatat taaatctcct cgacaataca ttgcagcgga tgcccttcgg
    16741 cttttgcccg ctgcatgact tgttgttgtt tggtttgggc aatatcgcgc gtgtaagtgc
    16801 cgcacaggcc tttgccttcg tgatgaacca agagcattac cgctaccgcc tgttcttgtc
    16861 cgagcataaa gatttcggtc aggatttcga cgacaaattc catcgtggtg taatcgtcgt
    16921 tcaataggaa aacgccgtaa cgtttcggcg gcagggtgtt cagacggtgc aagagcgtgt
    16981 cggattggtg ttgcgcggtc atagtgtgtc ccatttgaaa gccgcgttca gacggcattt
    17041 ttgttgtatt ttcggtactt ttgcctattt tcccactttt ttgaaaacat agcttgacgt
    17101 tttgtcttaa caaatgtaaa aagacggtta agcaggattg ggcatccgcc gagtatatca
    17161 agctggaagg aacgccagac ggctcggttt gccgtatgcc ttgttttgct gattttgtta
    17221 atttttgagt ataggaagtt tctaatggca accggtatcg taaaatggtt taacgacgct
    17281 aaaggttttg gtttcatcac gcctgatgaa ggcggcgaag atttgttcgc tcacttctca
    17341 gcaatcaata tggaaggttt caaaaccctg aaagaaggcc aacgcgtctc tttcgacgta
    17401 accaccggcc ctaaaggcaa acaggccgcc aacattcagg ctgcttaatt cctgatgtac
    17461 ggtcaaatgt atatttgaaa acggcgggac aggcaatgtc ccgccgtttt tgtctgccgt
    17521 tttgccggcg gcggaaaaac cccaatcccc gcacgcctta tcctgaactt gtgtgtaccc
    17581 tgttgtggac aagtggctta gtattttgac ggataaggga aaatcagtgc tgatgaaaaa
    17641 atgtgcaatg ttgtcggcaa aaggcggtgc ggcataaaac ggcaaacggg caggcacggg
    17701 gcaaaacgtg ctgccttcgt cttcagacgg catcggcagg gcgttcagct tccggcaacc
    17761 gtcatccccg caatcagaat cgagccgatt ttgttggacg aacgccgcaa agcgtcatcc
    17821 gccacgccga caatgtcgcg gtacatatcc tgcaagcgtc cggctacggt aatctcgtgg
    17881 acggggtagg caatcacgcc gttttccacc caaaaacccg ccgcgccgcg cgagtagtca
    17941 ccggtaatgg tgttcgcgcc ctgtcccatc agttcggtta ccaacaatcc cgtccccatt
    18001 tctttcagca ggtcggattg cgtttcgtgc gtatggttca aatacaggtt gtgcgcgccg
    18061 ccggcgttgc ccgtggtctg cataccgagt ttgcgcgcgc tgtaactgct gaggaaatag
    18121 ccttcgacaa tgccgttttg aatcacgaag cgcggcgcgg tggcaacgcc ttccgcatca
    18181 aaatagctgc tgcggaaaga gcgggggatg tgcggttctt cgcgcaggtt gaggaaatcg
    18241 ggcaggacct gtttgcctat gctgtcgatc aggaagctgc tttggcggta gagcgagccg
    18301 ccggacagtg cgccgacgag gtgtccgatc agacctcccg aaacggtcgc atcgaatagg
    18361 acggggtagc ttccggtcgg aatgctgcgg ctgttcagac gacgtaaggt acgttcggcg
    18421 gcggtttgac cgatttgcgt cgggctgtcc atgtcttgat ggcggcagga ggaatcgtac
    18481 cagtagtcgc gctgcatgcc gttttcgtcg gcggcaacga cgctgcatga aatgctgtgg
    18541 cgtgtgcctt gctggtgtgc ggcaaaaccg tgggtgttgc cgtaaacgta ttggtaatgt
    18601 ccggtttgca ccgccgcccc ttcggagttt tcgatgcgct catcctcgtt cagggcggct
    18661 tgttcgcatt gttttgccaa ctcgacggcg gcttccgtat ccaaatccca ttcgtggtaa
    18721 aggtcggggt cgccgatgtg ttgcgccata agcgaagcgt ctgccaaacc tgcgcaatcg
    18781 tcttcggcgg tgtagcgggc gatgtcgatg gcggctttga cggtgtcttg cagggctttt
    18841 tcggagaagt cggcagtgct ggcgcggcct ttgcgtttgc cgacgtaaac ggtaatgtcc
    18901 agcgacttgt cctgctggaa ctcgatttgt tcgatttcgc ccagccgcac gctgacgctt
    18961 tgtcccaatg attcgctgaa atcggcttcg gcggcggtcg cgcccgctgc ttttgccaag
    19021 tcgagcgtgc ggcggcagag gtcgagaagt tcggaagcgg tgtggttgaa cagcatagaa
    19081 atctttcttg gtggagcgtt gcggcatttt aaccgtttcg ggcggcaggg gcaaaagcgt
    19141 gccgtttgca gggcggacgg tgcaaaatgc cgtctgaacg cggaatcatt ctgttaaaat
    19201 gcgcccgtcg gataaaattt gagaatgaag atgtttgaac aagaagacga atgggtcagc
    19261 aaaacccaaa tgaaaaagca gatgaacggt ttgcaggatt tgggtatgga gctgaccaag
    19321 ctctcaagcg acacgttgaa aaaaatcggc ttggacgaag atttgtacga agccgtcgtt
    19381 acctataaaa aaatcacatc caacggcgcg ctcaagcggc aggcgcaatt tatcggcaga
    19441 ctgatgcgcg ataccgatcc cgcgcccatc gaggcatttc ttgccaagct gcgcggcgac
    19501 gatgcggtgc acaatgcctt tttgcaacgc gtggaacagg cgcgcgtacg gctgttggca
    19561 gacgacggcg cgctgacgca gtttatgtcc gattttccaa acgccgatgc gggcaagctg
    19621 aggacgctca tccgcaatac caaaaaagaa caggaacaga acaaaccgcc gaaaaatttc
    19681 cgcgccctgt ttcaagagtt gaaaaccgtt atggaaagcc aaggcggaac aggggaagcc
    19741 taggcatatt ttcagacggc atccgccgtc attctgattg gaagataaaa tgttgttccg
    19801 taaaacgacc gccgccgttt tggcggcagc cttgatgttg aacggctgta cggcaatgat
    19861 gtggggcatg aacgacccgt tcagccaaac gaccgcctat aaacacgttg acaaggatca
    19921 aatccgcgcc ttcggtgtgg ttgccaaaga caatgcccaa ttggaaaagg gcagtctggt
    19981 gatgatgggc gggaaatact ggttcgtcgt caatcccgaa gattcggcga agctgacggg
    20041 cattttgaag gcagggctgg acaaaccctt ccaaatagtt gaggataccc cgagctatgc
    20101 tcgccaccaa gccctgccgg tcaaactcga atcgcctggc agccagaatt tcagtaccga
    20161 aggcctttgc ctgcgctacg ataccgacaa gcctgccgac atcgccaagc tgaaacagct
    20221 cgggtttgaa gcggtcgaac ttgacaatcg gactatttac acgcgctgcg tatccgccaa
    20281 aggcaaatac tacgccacgc cgcaaaaact gaacgccgat tatcattttg agcaaagtgt
    20341 gcctgccgat atttattaca cggttactga aaaacatacc gacaaatcca agctgtttgc
    20401 aaatatctta tatacgcccc cccccttttt gatactggat gcggcgggcg cggtactggc
    20461 tttgcctgcg gcggctctgg gtgcggtcgt ggatgccgcc cgcaaatgaa cagcaatgcc
    20521 gtctgaaaag ctttcagacg gcatttcagc ctcatcatat cttgaaaaaa cctcgtcatg
    20581 tcagccaaaa tccaaacccg atccgtcaat accgacgttt ttaatcattt gctcaccgcc
    20641 ggtgccgatc ctttaatcgc ccagctttgt gcttcgcgcg gtgtgcaaag tcctgccgaa
    20701 ttggacgaca aactcgcttc cctcctgcct tatcaatcgt tgacgaattg cgaagccgcc
    20761 gcccgccgtt tggcggatgc ggttgggcgc aaggaaaaaa tcctgattgt tgccgactat
    20821 gatgccgacg gtgcgacggc gtgttccgtc ggtatgagcg gtttggcggc gatgggggcg
    20881 aaagtggatt tccttgtgcc caaccgcttt gagcacggct acggcttaac gcccgagctg
    20941 gctgaaatcg cggcagagca aggtgtggat ttgctgatta cggttgataa cggtatcgcc
    21001 agcatcgcag gcgtggcgcg cgcgcaggct ctgggtttgg atgtcatcgt taccgaccac
    21061 catctgcctg ccgagaccgt gcccgactgc atcatcgtca atccgaacca aaaaggctgc
    21121 ggttttccaa gcaaaagctt ggcgggcgtg ggcgtgattt tttatgtatt gatggctttg
    21181 cgtgccgaat tgcgccgccg caattatttt tcagacggca tcaaagagcc gaatctgggc
    21241 gaacttttgg atttggtcgc actcggcacg gttgccgatg tcgtccctct cgaccacaac
    21301 aaccgcatcc tcgtgtcgca aggtttgaaa cggatgcgct ccggcaaaat gcgccccggt
    21361 atccgcgcct tgtttgaagt ggcgcggcgc gattggcgca aggcgcagcc gtttgatatg
    21421 ggttttgcgt tgggcccgcg catcaacgcc gccggacggc tggacgatat gtcggtcggt
    21481 attgcctgcc tgttggcgcg agatgatacc gaggcgcagg aactggcggc gcagttaaac
    21541 aacctcaata tcgaacgtcg cgaaatcgag cagtctatgc tgcaagacgc gctgaatgct
    21601 tttcctgaaa ccctgccttc aggtcagatg actttggtgg cgtatcgcga cgacttccat
    21661 caaggcgtgg tcggcatcgt tgccagccgc ctcaaagacc gtttttatcg tccgaccatc
    21721 gtgtttgcgc ctgccgacaa cggtgaagtg cgcggttcgg ggcgttccat tcccaatctt
    21781 cacttgcgcg atgctttgga cttggtgtcc aaacgccatc ccgatttgat tttgaaattc
    21841 ggcggacacg cgatggcggc gggcttgagc atactcgaac acaacattcc cgcgtttcag
    21901 acggcctttg aagaagccgt gcgcgaaatg gtgtgcgaag acgatttgtc gcaaaccttc
    21961 atcaccgacg gcagcctgcc tgcacgcgac attacgctgg aacaggcgca aaaccttgct
    22021 aaacacgttt gggggcaggg cttcgcgccg ccgagtttta ccgacgagtt ccacgtcgtc
    22081 cgccagcagc ctttgggcgc ggaaggcaaa cacaaaaaag cgtggctgca aaaagacggc
    22141 tgcgaatttg aagcaatgtt ctggcggtgc agcgaagaca ttcccgaata catccgcacg
    22201 gtgtaccgcc ccgttgccaa cgaatggcgc aacaacctcg aattgcagct ttatatcgac
    22261 tactgggaag cggcgtagag gcggcggaac gccgtttgaa tgtgattttt gttccttcat
    22321 ttgcctgttt gtacgacggg aatgttccca atcggaaaag aaagtaaagg gcgttaattt
    22381 ttgttaatca tcccttctta gggacgcaat atataaggtg catcaaattt cagactccgg
    22441 cggatttgca tttgaagtgc aactttccct aacagaaaaa ggccagtatg cggtagcata
    22501 cg
//
LOCUS       DAXHPG010000014        22342 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXHPG010000014 DAXHPG010000000
VERSION     DAXHPG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22342
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.014"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            1..99
                     /locus_tag="ACFM6F_000395"
                     /pseudo
     CDS             1..99
                     /locus_tag="ACFM6F_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(120..1034)
                     /locus_tag="ACFM6F_000396"
     CDS             complement(120..1034)
                     /locus_tag="ACFM6F_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6163877.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1302..2771
                     /locus_tag="ACFM6F_000397"
     CDS             1302..2771
                     /locus_tag="ACFM6F_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HFC6163878.1"
                     /translation="MDKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3023..3394
                     /locus_tag="ACFM6F_000398"
     CDS             3023..3394
                     /locus_tag="ACFM6F_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163879.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3467..4876
                     /gene="thrC"
                     /locus_tag="ACFM6F_000399"
     CDS             3467..4876
                     /gene="thrC"
                     /locus_tag="ACFM6F_000399"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC6163880.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGSAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLETLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4923..5717
                     /locus_tag="ACFM6F_000400"
     CDS             4923..5717
                     /locus_tag="ACFM6F_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163881.1"
                     /translation="MTLPMQETHFSILLDELAAKQEAAIAPHLLADGTKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDE
                     ENISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSEQP
                     AEIQRLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADIPP"
     gene            5905..6681
                     /locus_tag="ACFM6F_000401"
     CDS             5905..6681
                     /locus_tag="ACFM6F_000401"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6163882.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6757..6863
                     /locus_tag="ACFM6F_000402"
                     /pseudo
     CDS             6757..6863
                     /locus_tag="ACFM6F_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7442..9913)
                     /locus_tag="ACFM6F_000403"
     CDS             complement(7442..9913)
                     /locus_tag="ACFM6F_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6163883.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9910..11088)
                     /gene="hflX"
                     /locus_tag="ACFM6F_000404"
     CDS             complement(9910..11088)
                     /gene="hflX"
                     /locus_tag="ACFM6F_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC6163884.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11093..12373)
                     /locus_tag="ACFM6F_000405"
     CDS             complement(11093..12373)
                     /locus_tag="ACFM6F_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC6163885.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHASYLTLNPEDGHGEHHPDNPHYTAQKLT
                     EHTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            12375..12503
                     /locus_tag="ACFM6F_000406"
     CDS             12375..12503
                     /locus_tag="ACFM6F_000406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163886.1"
                     /translation="MPSSRRGNGVFYQNGKLANAVSDCRLPNRQTFPRADAEPDAV"
     gene            complement(12526..13251)
                     /locus_tag="ACFM6F_000407"
     CDS             complement(12526..13251)
                     /locus_tag="ACFM6F_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC6163887.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13324..14001)
                     /gene="radC"
                     /locus_tag="ACFM6F_000408"
     CDS             complement(13324..14001)
                     /gene="radC"
                     /locus_tag="ACFM6F_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC6163888.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQSVCSF
                     RQLGLMP"
     gene            14028..14150
                     /locus_tag="ACFM6F_000409"
     CDS             14028..14150
                     /locus_tag="ACFM6F_000409"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163889.1"
                     /translation="MPSETDVPTRQISEMPSEPDFRFRRHLYFKTVLRRFFQLD"
     gene            complement(14124..15473)
                     /gene="gshA"
                     /locus_tag="ACFM6F_000410"
     CDS             complement(14124..15473)
                     /gene="gshA"
                     /locus_tag="ACFM6F_000410"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC6163890.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHCPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNVYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15733..17142
                     /gene="leuC"
                     /locus_tag="ACFM6F_000411"
     CDS             15733..17142
                     /gene="leuC"
                     /locus_tag="ACFM6F_000411"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC6163891.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLISVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMESR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17239..17493
                     /locus_tag="ACFM6F_000412"
     CDS             17239..17493
                     /locus_tag="ACFM6F_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163892.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17555..18196
                     /gene="leuD"
                     /locus_tag="ACFM6F_000413"
     CDS             17555..18196
                     /gene="leuD"
                     /locus_tag="ACFM6F_000413"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC6163893.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAGQTLT
                     TPGGETLAFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18365..19636
                     /locus_tag="ACFM6F_000414"
     CDS             18365..19636
                     /locus_tag="ACFM6F_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6163894.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19652..20383
                     /locus_tag="ACFM6F_000415"
     CDS             19652..20383
                     /locus_tag="ACFM6F_000415"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HFC6163895.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNASPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20417..21487
                     /gene="leuB"
                     /locus_tag="ACFM6F_000416"
     CDS             20417..21487
                     /gene="leuB"
                     /locus_tag="ACFM6F_000416"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC6163896.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKEYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21650..22213
                     /locus_tag="ACFM6F_000417"
     CDS             21650..22213
                     /locus_tag="ACFM6F_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC6163897.1"
                     /translation="MKKIIIAALAAAAIGTTSAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPNIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMLKTEVCGGDFSTTIDRT
                     KWGMDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5389 a         5885 c         5798 g         5270 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agtttttcgg atgatgatta ttaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtga cggcttcctt tcggtggccc gaacgcagcc catatcaaaa agaaaagtca
     1261 tgcgcccctt ttgcgaggcg cgatatataa ggaggaagat tatggacaaa cataatggga
     1321 cttatcggga tttgcaccgt ccggcttcgg aatttgcgac gcgggacgaa tatttggaac
     1381 atgaattgca gattatgcaa ccaaaacgct ggcggcccaa cctgcccttt cgcgattacc
     1441 gcttcgagtg ggaggatttg attcctgcga tggcgggaac gattggaaaa gtggtgatgg
     1501 tgggggcggt ggcggcggcg tttgccgcac ctttggggct gcctgacagc tttgtactgg
     1561 aaaatgtgcg ctatgagctt ttaatcgccg ccgcgtttat cttattggta tcgggctttt
     1621 ttctgcccgg cgccaacctg cccggtacgc acgggccgct gattccgatg attcccatcg
     1681 ttgtgtcggc aggcgggcat cctttggcgt tcggcatttc gattgcggtt ttaggtctgc
     1741 tgatggcttt atttcgcggc ggcagtatta tggcgaagct gacaagcaac ggcgtatgcg
     1801 gcggattatt actctatttg ggctttatcg gcacgacggg gcaggtaaaa aaattgtttt
     1861 cgtgggcagg cggttttaat atgccctaca tcgcttttac cgtcattatt gtaacgattg
     1921 tgatgtacgc tttgttggag cattggaaaa aacgctggtt agccgtgcct ttgggatgct
     1981 tgattgccgg tgtggtggca tttgcattgg gtgcgccgtt tgagtttcac accgcccccg
     2041 gcctgcctcc aatgagtcct gcttattggt ggggtgaaaa cagcggctgg catctggggt
     2101 tgccgacggc agaaagtttt ttggttgtct ttccatttgc ggtattggct gttgcaatgt
     2161 ggtcgcccga ttttttagga catcaagtgt tccaaaaaat cagctatccg gaaaaaaccg
     2221 ataaggtatt gatgaatata gacgacacca tgacaagttg ttctgtccgt caagcagtgg
     2281 gttctatttt agggggtgca aattttacct cttcttgggg aacttatatc gtaccggcat
     2341 cgattgccaa acgccccatt ccgggcggtg cggttttaac ggcggtttta tgtattatcg
     2401 ccgggttatg gggctatccg atggacttgg cgatttggca gccggtattg agcgtagcct
     2461 tggtcgtagg cgtatactta ccgcttttgg aagcgggcat ggaaatgacg cgcaaaggca
     2521 aaaccaccca atccgccgcc atcgtggtgt tctcttccgc cttggtcaat ccggttttcg
     2581 gctgggcgtt gacgatgctg ttggataatt tgggcttaat cggctgcaaa gagcgcagtg
     2641 cgcaattagg tttcgccgga cgcgtgttga tacccgcagt aggtttcttg atcttgtgtg
     2701 tggcgatggg tgcggtcggg atgctgcccg gtatcccgcc gtttttggaa cacttcaaat
     2761 ctttgggcta ggctgaaatc ggaaatgccg tctgaaccgc tttcagacgg catttttgca
     2821 aacaggcaaa atgacggcgg cgggattttt tattttcccg attgaagtat aatgttgccg
     2881 ggcttcaacc ggatattcaa aacggtttgt tccaacactc ggaacggcgc ataaaacgcc
     2941 gcccttcgcg ttatcccgaa cggggcggct aatcagattc tatcgccata aaaggcgggg
     3001 tttcaaccga aaaggaattg agatgaataa aaccttgtct attttgccgg tggcaatctt
     3061 actcggcggc tgcgccgccg gaggcggtaa cacattcggc agcttagacg gtggcacagg
     3121 catgggcggc agcatcgtca aaatggcggt tgggagccaa tgccgtgcgg aattggacaa
     3181 acgcagcgaa tggcgtttga ccgcgctggc gatgagtgcc gaaaaacagg cggagtggga
     3241 aaacaagatt tgcgcttgcg tcgcccaaga agcacccgaa cggatgaccg gcaacgatgt
     3301 gatgcagatg ctggctccgt ccacgcgcaa tcaggcactt gccgccctga ccgccaaaac
     3361 ggtttccgcc tgcttcaaac acctgtaccg ctaaccttct tcagacggca tgaggcagat
     3421 gccgtctgaa gccgttttcc tgcaatttcg acagacgaga aaaatcatga aatacatcag
     3481 cacgcgcggc gaaaccgcac acaaaccgtt cagcgaagtt ttattgatgg ggcttgcccc
     3541 cgacggcggt ctgatgctgc cggaacatta tccgcaaatc gggcgcgaaa ccttggacaa
     3601 atggcgcggt tcggcttatc ccgaattggc gtttgaaatt atgcgcctgt tcgttacgga
     3661 tattccggag gacgatttgc gcgatattct gaaccgtact tacacggaag cggcgttcgg
     3721 tactaaggaa atcacccccg tccgcacgct ttcagacggc atcaaaatcc aagccttgtc
     3781 caacggcccg acgctggcgt tcaaagatat ggcgatgcag tttttgggca atgcgtttga
     3841 atatgtttta aacaaagaag gcaaaaaact caatatcttg ggcgcaacca gcggcgatac
     3901 gggttcggct gcggaatatg ccttgcgcgg caaaaaaggc gtgaacgtat ttatgttgtc
     3961 gcccgacggt aagatgagcg cgttccaacg cgcgcagatg tacagcctgc aagacgggaa
     4021 tatccacaat atcgccgtga agggtatgtt tgacgactgt caggatattg tgaaggcggt
     4081 gcagaacgat gccgcgttca aggaaaaata ccacatcggt acggtcaatt cgatcaactg
     4141 gggacgcatc gtcgcacaag tggtttatta ctttgcgggc tatttcaaag cgacacaaag
     4201 caatgacgag caagtcagct tctgcgtgcc gagcggcaac ttcggcaacg tttgcgcggg
     4261 acacatcgcc aaacagatgg gtttgcctat ccgccgcctg attgtcgcga ccaatgaaaa
     4321 cgatgtgttg gacgagtttt tcaaaaccgg tgcataccgc ccgcgcaaca gcgcgcatac
     4381 ttatgttacc tccagcccgt cgatggacat ttccaaagcg tccaacttcg agcgtttcgt
     4441 gttcgacctg atggatcgcg atcctcagga aatcaatacg ctgtgggcgg aagtagcggc
     4501 gggcaaaggc tttgacctgc ggtttgcttt agaaaaagtc ggtggcaaat acggttttac
     4561 ctccggcaaa tccacccacc ccgaccgcct cgccaccatc agacaggttt atgagcaaga
     4621 ccaagaactc atcgacccgc acactgccga cggcgtaaaa gtcgcccgcg aagtgcgcga
     4681 agaaggggaa acggttgtgt gtttggaaac cgcgttggca gcgaaattcg atgcgaccat
     4741 acgcgaagcc gtcggcgatg ccgccattcc gcgccccgcc gcgctggaag gtttggaaac
     4801 attgccgcag cgcgtgcaaa ccgtgccgaa cagtgcggat gcggtaaaag gcatcatcga
     4861 acaaaccctt gcctgatgtt ccgtatgggc ggcattccgt ccgaacttaa gattgaaaga
     4921 aaatgacctt acctatgcag gaaacccatt tttccatact gctggacgaa ctggcggcaa
     4981 aacaggaggc agccatcgcc ccccacctgc ttgccgacgg aaccaaggta tggatacgca
     5041 aagcaggcag gcacaatgcg cgatggcgtt acgcgctgct cggtatggtt gcccgatatt
     5101 tgaaactggg ggtgttgaag ccggttccca gcctcggcgg cgagcctgcc attgcaaccg
     5161 aatcaaaacg cctgtacgaa ttgcgcgcgg cggggatagc cgttcccgaa ttgctcgccg
     5221 tcaggaaaaa tgcccttatg ttcggaaatt tggaaggtat cccgctcgat acgcaaatcc
     5281 gccaagaagc cgaagccgga aaggcggacg cttggcttgc cggtttggaa gccattgcac
     5341 gcgtgcataa aaaacagcag ttcctcagtc aggcgtttgc gcggaatatg atgtcggacg
     5401 aggaaaacat cagctttttg gattttgaag acgacccttc cgaagtcctg accatagcgc
     5461 aatgccaagc ccgcgattgg ctctgctaca tccattcgac cgcgctgata ttgaaaaacg
     5521 gcggactgct ggaagcggct gcggaaaaat ggggcggcgt actgtctgag cagcctgccg
     5581 aaatacaaag gctgattgcc gccaccgtca aaccgatcct cccgatacgc aggctggaac
     5641 acccccgctg gggacgggac gcgctcaggc tggcagcctc gatttccctg atttcccttg
     5701 ccgatatacc gccataagcc gccgcccgcc ccgcgatgcc gtctgaagcc cttcagacgg
     5761 catataacca acgcgcatca agcattgtcg caaccatccg tttcgtttag aattttggct
     5821 ttatcccgaa tcgggcagac atttccactc tgccgcacgt cccgaacaag cactatccat
     5881 ttcttttact gacaggaaac ccaaatggca gcattcaata cccaaaaagt attgtccgta
     5941 caccactgga cagacgcata ttttaccttt acctgcaccc gcgacgaatc gttgcgcttt
     6001 gaaaacggac agttcgttat ggtcggattg atggtggacg gcaagccgct gatgcgtgca
     6061 tacagcgtcg cctccgccaa ctgggaagaa cacctcgaat ttttcagcat taaagtccaa
     6121 gacggcccgc tgaccagccg cctgcaacac ctcaaagtcg gcgacgacgt gttaatcagc
     6181 aaaaaaccga ccggaaccct ggttgccggc gacctgaatc cgggcaaaca cctttacttg
     6241 ttgagcaccg gtaccggcat cgctcctttc ttgagcatca ccaaagaccc cgaaatttac
     6301 gagcaatttg aaaaaatcat cctcgtacac ggcgtgcgct acaaaaaaga tttggcgtac
     6361 tacgaccgct ttaccaaaga attgcccgaa cacgaatacc tcggtgactt ggttaaagaa
     6421 aaactgattt actacccgat tgtttcgcgc gaagaattcg aacaccacgg tcgcctaacc
     6481 gacctgatgg tgagcggcaa actgtttgaa gacatcggcc tgcccaaaat caacccgcaa
     6541 gacgaccgcg ccatgctgtg cggcagcccc gcgatgctga aagatacctg caaagttttg
     6601 gacgatttcg gactgaccgt ctctccgaaa acaggcgtac gcggcgatta cctgattgag
     6661 cgtgcgtttg tagatcaata agctgtttga aaatcccgtc tgaacggcac cagcaatcaa
     6721 aaaccccgaa tcctgccaat caggattcgg ggtttttata gtgaattaaa tttaaatcag
     6781 gacaaggcgg cgaaccgcag acagtacaaa tagtacggca aggcgagcca acactgtacc
     6841 ggtttaaatt taattcacta tatagtgggt aaaaattaga aatgcacatt tccgtcattc
     6901 acgcgcaggc gggaatccag acctatccgt taattagcat ttgaaaataa aagaaaaacc
     6961 aagcatatgg attcccccct acgcgagact gacgacggtg ttctgcagta gccaaagcag
     7021 aaaaccatcc ttattgtcat ttcatcatga tataacaata aggatgttaa aaattagtta
     7081 tcattatatt catgatatat aagaaaaatt gtcccaataa gaaatgttat gcagaaaata
     7141 aaatgaaaag gatggttttc attgaaaatg gttgttctgc caccgtcata cacagaaccg
     7201 gtatttggaa aaaaaataat ccatattgac atcattattc catttatcgc ataaatccac
     7261 tttttaattt ttttcctcat taatacaatc tcttatttaa taaaatcaaa tacctagcaa
     7321 ttcgatctca aattttgaac gccaacgtgt ttgagcggta acaatacata ctttaagttt
     7381 gcaattcttc atcgtcttat ttttgttttc atcaactata cgtctaaagc aacgccgtcc
     7441 gtcattgttc ggacggcatt ttttcagact gcattttccc ccgtttgtga aggcgcaggg
     7501 cgttgcccaa aaccgccagc gaactgaagc tcatacccag tgcggctatc cacggttgga
     7561 catagcccaa aacggcaagc ggtacggcaa tgatattgta cgcgcccgcc catatcaggt
     7621 tttgccggat aatatggcgg gtgcgccgcg cctgatcgag caggtgggcg acggtacgca
     7681 aatcttcgtt caataacaca atgtccgcgc cgtccctcgc aatatccgtc ccgcccgctg
     7741 cggcggcgga tacgtctgcc tgcgccaaaa cgggcgcgtc gttgatgccg tcgcctatca
     7801 tcagcacttt tttcccttct ttttgcaagg ctttgacgta ttccagtttg tcctcgggca
     7861 tggcttgggc gcggtagtgc gcgacaccca gggcgcgtgc ggtttcggca acggcggttt
     7921 cgcggtcgcc gctgaggatg tgcagggtca ggtttttgcc tgccaactgc cgcaccgcct
     7981 ccgccgcgct gtctttcaag gggtcggtca ggtagaacac ggcttggaaa ccgctttgac
     8041 tgccgaggta aaccgcgctg ccgccgcctt ctgtttgcgg ttctttacct gaaatttcgg
     8101 cgacatagga tgccctgccc aatgcccaaa cctgtgtttc gccgttgacg gtcagttgcg
     8161 cgcccacgcc ttcgccgatg cggttgaggc gttgtttaat agcgatgtcg gggacgctgc
     8221 cgtctgaaat gcggcagttg aggatggcgc gggcaagggg atgttcggac tgttgttcta
     8281 aagcctgcgc caccgcgaga acaaaggctt cgtctgtgcc tctcaacaat gagatacggc
     8341 ggacggcggg tttgccttgg gtcagcgtgc cggttttgtc gaagatgatg tcggtggttt
     8401 gggcgagggt ttcgattgcc tgctttccgc cgattaaaat accttcgcgc gccagcgtac
     8461 cggtagaagc tgccagcgcg gtcggcgtgg caagcgataa ggcgcagggg caggtaatga
     8521 ccagcagggc gacggtaatc cacaatgcgg tgtgcgcgtc ggcgtacagc gtccagccga
     8581 tgaagacggg gacggcaagc aggagttcgc cgaatatgaa agacgaggcg tattgttccg
     8641 ccaactcggc agtgcgcggt ttttgcgcta aggcgcggtc gagcaggcgg acgatgtgcg
     8701 acagtcgcgt gccaccgccg gtgcggtcgg tgcgtataat cagggggctt tgcgtgttga
     8761 gtgtgccggc ggttactttt tcagacggca ttttggcgac gggcaggctc tcgccggtca
     8821 gcatagattc gttgacggca ctgcttcctt ccagcaccgt gccgtcaacg gggatggttt
     8881 cgcccggttt gaccagcacg atatcgcccg ctttcaattt gacgacagct gcctcgcagg
     8941 tttcctgcgt atcggggtaa tcgggcatat gatggcaaaa cgcaggaatc agcttcacca
     9001 gcctctcggc ggcatcgcct gccttacggc gggcaatgtg ttccataaag cgtccgccca
     9061 gcaggaaaaa cagcagcatc gcgatggatt cgaaatacat cccctgcccc gcatttgtcg
     9121 caaggctgta aacgccggcg ataaaggtca tgatgatggc gacggtaatc ggcgtatcca
     9181 tgccgacgcg gcggtttttc aagtcgcgca gcgcgccttg ataaaacggg acggcgcaat
     9241 agaatacgac gggcagcacc attaaaaagc cgccccaatg gaggatttgc aggaaatcgg
     9301 gttcgatgtc gccgccgtaa aggtaggtcg gcagcgcgaa catcatcgtc tgcatcatcc
     9361 ccagcccggc aacggcgagg cggacgatgt attgtttgcg ttctttttgg ttggcggctt
     9421 cgattttttg cgcgtcatag ggtgcggcgg tgtagcctat ctgcctgatt ttcaacagaa
     9481 tgtcggaaag gcggattttg ccgtcgtccc agacgacgcg gcagcggtgc gtgctgtaat
     9541 tgaggtcgat gcggacgatg ccgtctgtac gcaaaagctg ctgttcgatc agccagacgc
     9601 aggcggcgca ggtgatgccg ccgagcatta aaaccgcctc gcgcgtgccg ccgtgggttt
     9661 ccacaaagtc ggactggact tcgggcaggt cgtacaggcg gatttggtcg aggatttctt
     9721 ggggcggcag ctcggttttt tgcgcgtcgg cggtgcgttg tttgtaataa ctgcccaagc
     9781 ccgcgtcaat aatgctttgt gcgaccgcct gacagccggc gcagcaggtt tcgcggtctt
     9841 cgttttcgta acggacagtc aggtggaggt gttcgggaac atccagaccg cagtggaaac
     9901 aggttttttt catggcattt cggtttcgtc tgtgtttggt gcggcggcac aagactcggc
     9961 aatggcttcg cgcagtgcgt ctataccggt attttcagca acggaaatgc ggacggcggc
    10021 aatttttccc gcagcgtcgc gccatatgcc cgtgttttgt tcttcagacg gcagcaggtc
    10081 ggttttgttg tacaccttga tgcacggaat atcgccggca tggatttctt gcagtacgtt
    10141 ttccacgtct tcaatctgct gtccgctgtt cggagcggcg gcatcgacga cgtgcagcag
    10201 cacatcggct tgcgcggttt cttccagcgt ggcggaaaag gcggaaatca gtttgtgcgg
    10261 cagatcgctg acgaatccga cggtatcggt caggataatg ctgcattcgg gactgatgta
    10321 cagccgccgc gccgtcgtgt cgagtgtggc gaaaagctgg tctttcgcat atatgcccga
    10381 cttggtcagc cggttgaaca ggctggattt gccgacattg gtatagccga ccagcgcaaa
    10441 cgttttgatt gtgcccgatt cgcgggactt gcggcgcagg gcgcgctgtt ttttgaggtt
    10501 ggcaagctgt tttttcaagg cattgatccg atgggcgatc aatcggcggt cggtttccag
    10561 tttggtttcg ccggggcctt tcatgccgat accgccgcgc tggctctgca gatggccgta
    10621 accgcgtatc aagcgtcccg ccaaatggct caattgcgcc aactcgactt gcagcctgcc
    10681 ttcctgcgtg cgggcgcggc gagcgaaaat cgccagaatc agccctaccc tgtccaatac
    10741 gcggcatttc agttcttttt caaggttgcg ttcctgcgtg ggcgtgagtt cgtggttgaa
    10801 tacgaccaaa tcgatgccgt ctgcggcaac tgcttctgac agctccgccg ccttgcccgt
    10861 gccgacaaac agcgcggtgt gcggacggtc gcgtttggca gtctccacgc gcacggaatc
    10921 gccgcccgcc gctttgacca gctcgacagc ttccgccaat gccgtctgaa aaccgttcag
    10981 acgggcggca ctactgcccg taccatcttt gtccaacatt acgcccacca gcatgacgcg
    11041 ttcgggttgc gcttgggtac tgccgttgcc gcctgttctg cctgtcatgt attcaatcct
    11101 gtaaataaag ggttacgcct cgttccgttt ctccttccgg tgcaagcgtg atgcggctgc
    11161 ccgccattcc gtcaacgctg aagacgatgc cgcccgcctc gtgccttcct atggacaggc
    11221 ggctgccggg tcgctgccgg taggtataac gggtacacgc ttccaaacac cagcgtccgc
    11281 gccagtggat gaaatatttt tcgccttttc taaccgcaag tgtctcgccg ccgtttacct
    11341 caaaataagg gtaatcgggt tttcgggttc taaactgcca tttcgcctgc gctcgccgtc
    11401 cgttgcggac atagtcgaat accgccgtat aaagcgtgcc gtattccaaa ggcttgagcg
    11461 gaaaaagcgc gaattggtac gcggtcagcc tgccgttggg gtcgttgccg gcggttaaaa
    11521 ccctgacggg gcggatttcg tttttaccct gatacagctt gaaacttttc atcgtaattt
    11581 tgcctgccgc ctcggaaaaa tcaatgctgg caggattgcc cgtgatttca tattccggca
    11641 cggggtctgg acgctcgccg tggaaataag gcagtgcgcc gctgccgacg ggataggctg
    11701 tatagagcag ctcctgtgcg ggcatggctt cgtcggtgta cacgaccgca ccgttatggc
    11761 aggcgttgcg gtaatatttc cgtcctgctt ccggctgatt tctgccttgg gcgcaatgcc
    11821 gctcaaacct gccgttgccc tgattgaata cgagaacggt tttaccgttt tcgcgcacaa
    11881 atgccgctcc tgcctcatcc gtatggcggt caagtaggga aaggcggtgg tagattgcgc
    11941 tcattaatcc gtccacttgg cgttgctgcg tgcggatgtc gctgtcggac gattcggcgg
    12001 cttcttcttc cgtgctgatg ttttcatgca cgccgttgta gagatacccg gcaaggcgtg
    12061 tgtgttcggt cagcttttgt gcggtgtagt gcggattgtc gggatggtgt tcgccgtgtc
    12121 cgtcttcggg attgagcgtg aggtagcttg cgtgcctgcg ggcggagttt tccaaaaccg
    12181 gcgcgtgtgc cagcttgtgc aaaccgattt gggctcggat gcggttgagg tagtgcagcg
    12241 catcgccgcc gtccctgatt tgcggtgcgg acggatagac aagttcgccc gcgggcaggg
    12301 attggttttg ggtatggtag aaaacgcctg ccgccgaacc caacaggagc agccgaataa
    12361 aaagggattt cataatgccg tcctcccgac gcggcaacgg ggtgttttat caaaacggca
    12421 aacttgccaa tgcggtttcc gattgcagat tgccaaaccg gcaaaccttt ccccgtgccg
    12481 atgccgaacc cgatgccgtc tgaaccttca gacggcatcg ggtgtttatt tgtccacccg
    12541 gatgggggca ggttcgtatt gtgtcgattc gtcgccgtaa tacagcacgc cgagtttgat
    12601 ggggattctg ccctgtgatt tgcggtgggc attggaatcc ctcagggaat aggcacaacc
    12661 gcaatattcc tgctggtaga agttttcacg tttgctgatt tcaatcatgc gcgcgctgcc
    12721 gccgcctttg cgccagttga aatcccaata caccacatca tcgtaaggcg cggcggcgcg
    12781 gtgtccgcag tcgttgattt gcgccatatt tttccagcgt gaaatgccca gcgaactggt
    12841 aaagacgggg aacccgtgtt catgcgcgta ttgcgccgcc ttttcaaaac gcatatcgaa
    12901 acacatggtg cagcggatgc cgcgttccgg ctcaaactcc atgcctttgg ctttggcaaa
    12961 ccattctttg cggtcgtttt cgtagtcgtc gtctttatcg atgaaaggaa tgccgaactt
    13021 ttccgcaaag cgcacgtttt cctcttttcg gagcatatac tctttgtgcg gatggatatt
    13081 gggattgtaa aaataaatgg tgtagtcgat gccgctggcc agcatggctt ccatcacttc
    13141 gccgctgcac ggggcgcagc aggaatgcag caagactttt ttatgtccac cgggcgggac
    13201 gagtataggg cggtcaatgt cggtaacggt aggtttgttt tgtgtttcca ttaacttaac
    13261 aatatctgtt gaatacggtt gaagcggcta ttttatcaga gccgccgcat gtaaaacaga
    13321 gtgtcagggc atcaacccga gctgtctgaa cgaacagacg ctttgcgagg taacgataaa
    13381 atggtcgagc agcgacacat cgaccagcga cattgcctgt gccagccgcc ttgtgaacat
    13441 gatgtcttcc tgcgaaggtt cgggcgagcc gcccggatgg ttgtgcgcaa taatcaggct
    13501 gtcggcatat tcgtccaatg ccagtttgac gatttcgcgg atgtaaatcg tgttttccgc
    13561 aaccgtaccg cgcgacagct ctctgaccgc aatcagttgg ttttggcggt tcagcagcag
    13621 cgcgacgctg acttcgactt tttcctgccc caaatgaaag cgtaaataat cggccaccgt
    13681 atccggatcg gacaggacga tgctctcctg caattcttcc gccaatatcc gccgcccgat
    13741 ttccctgacc acggcaaact gtgtgaaact tgccgtcccc atccctttgt atgccgacag
    13801 tttgccgacc tccgcgctca tcagcctccc caaactgccg aactcctgca gcaaataacg
    13861 cgccaaatcg accgcactca ttccgcgcgt gccgacgcgc agcaggattg ccaaaagttc
    13921 ggcatcactc aacgccgccg ccccgcgttc caacagcttt tccctgggtc tttcgccttc
    13981 cggccattgc ttgatgctca tgttttccct tttttaaata gtctgagatg ccgtctgaaa
    14041 cagatgttcc cacacgccaa atatccgaaa tgccgtctga accggatttc cgcttcagac
    14101 ggcatctgta tttcaaaaca gtcttacgcc gtttcttcca actcgattga agccgccaga
    14161 agcgacaggc gtgccattac gccgtaaacg tagaggcggt tgtgttcgtt gtccacgtcg
    14221 cagtcttttt cagagcgcgg catacccagc gagtcccatt gttcgaatac gcgcttgcag
    14281 gcttcgggcg cttcttggga attatcgccg ttaccggtag gaatgctgtt agacagcgga
    14341 acaaacacca taccgccggc gttgaggttt tcgtccgcac cgcgcccttc gtgtacgcgg
    14401 aaaaagccgc cgatgacgaa gcggtccatc atatacacga cgggttcgca caccgcgccg
    14461 tttaaggttt cataagtata aatcccttct tggacaatca cttcgctgac ttccaagcct
    14521 tctttgactt tcgccatttt attgcggttt ttacggttca atccgcgcac ttcgtcggcg
    14581 gatttgacgc tcatcacgcc catgccgtaa gtgccggcat cggctttgac gatgacgaaa
    14641 ggtttgtcgg taatgcccga ttcgtcgtat ttggcttgaa ttttcgccag cacacgttct
    14701 accgcttccg ccaacgcgtc ttcgccttca cgcccttgga agtccaaacc gccgattttt
    14761 tcaaaataag ggttaatttg ccattcgtcg atgccgatta acttggcaaa ttcggcggta
    14821 acttggttgt acgcgccgaa atgatttgtt ttgcggcgcg tcgtccaacc gccgtgcaac
    14881 ggcggcaaaa cggtttggct gatgcctttg aggatgtcgg gaatgccggc ggacaaatcg
    14941 ttgttcaaca aaaccacgca aggcgaaaag ccgtctgcaa gatggacgcg atcgcgggta
    15001 cgcaataaag gttccaacag gattttgtcg cccaatgcgg tttcaaattc ggtcggttcg
    15061 gttacttccg gattcaggct gcccaagcgc acttcatacc ctgccgaacg caaaatctcg
    15121 ccgagggcgt aaacgttttg caggtaaaac gtattgcgcg tgtggttttc aggaataatc
    15181 aatacggatt tcgccgtttc gcaggcgcgt tgcaccgcat cttgcgcggc aaccgccgcc
    15241 agcgggataa agttgggatt cagattattg aagccgccgg ggaacaaatt catatcgata
    15301 gacgaaattt tgtaaccggc attgcgtata tcgaccgaac cgtaaaacgg cgggcagtgt
    15361 acgctccact gcgtgcggaa ccacgcctcg attttggcgt gattggacag gatttttgcc
    15421 tcaaacgcct gaagttgcgc cgaatgttcg ggcgacatca ccggtaattt catctttcgt
    15481 ctgcctctgt gtgttttttg acaagatgca gatgataatg cctttgaaag aatgacgaca
    15541 agtattcttt cgtatcgaat catgctttag atataactgc ttactgaaat ggacaatatg
    15601 tctaattttt ttgttaaaat tgtttacaaa ataacaccga ctcaaaaatt agacaaaatc
    15661 tgttgcgcgg tataaagaat acgtctaaaa tccgcccaat ccgcattacc atttatccaa
    15721 aagaacaaca tcatgacagc acaaaccctc tacgacaaac tttggaacag ccacgtcgtc
    15781 cgcgaagaag aagacggcac cgtcctgctc tacatcgacc gccatttggt gcacgaagtt
    15841 accagccctc aggcatttga aggcttgaaa atggcggggc gcaagctgtg gcgcatcgac
    15901 agcgtcgtct ccaccgccga ccacaacacc ccgaccggcg attgggacaa aggcatccaa
    15961 gacccgattt ccaagctgca agtcgatact ttggacaaaa acattaaaga gtttggcgca
    16021 ctcgcctatt ttccgtttat ggacaaaggt cagggcatcg tacacgttat gggccccgaa
    16081 caaggcgcga ccctgcccgg tatgaccgtc gtctgcggcg actcgcacac ttccacccac
    16141 ggcgcattcg gcgcactggc gcacggcatc ggcacttccg aagtcgagca caccatggcg
    16201 acccaatgta ttaccgcgaa aaaatccaaa tccatgctga tttccgttga cggcaaatta
    16261 aaagcgggcg ttaccgccaa agacgtggcg ctctacatca tcgggcaaat cggcacggca
    16321 ggcggtacag gctacgccat cgagtttggc ggcgaagcca tccgcagcct ttctatggaa
    16381 agccgcatga ctttatgcaa tatggcgatt gaggcaggcg cgcgctcagg catggttgcc
    16441 gtcgaccaaa ccaccatcga ctacgtaaaa gataaaccct tcgcacccga aggcgaagcg
    16501 tgggacaaag ccgtcgagta ctggcgtacg ctggtgtctg acgaaggtgc ggtattcgac
    16561 aaagaatacc gtttcaacgc cgaagacatc gaaccgcaag tcacttgggg tacctcgcct
    16621 gaaatggttt tagacatcag cagcaaagtg ccgaatcctg ccgaagaaac cgatccggtc
    16681 aaacgcagcg gtatggaacg cgcccttgaa tacatgggct tggaagccgg tacgccatta
    16741 aacgaaatcc ccgtcgacat cgtattcatc ggctcttgca ccaacagccg catcgaagac
    16801 ttgcgcgaag ccgccgccat cgccaaagac cgcaaaaaag ccgccaacgt acagcgcgtg
    16861 ttaatcgtcc ccggctccgg tttggttaaa gaacaagccg aaaaagaagg cttggacaaa
    16921 attttcatcg aagccggttt tgaatggcgc gaaccgggct gttcgatgtg tctcgccatg
    16981 aacgccgacc gcctgacccc ggggcaacgc tgcgcctcca cctccaaccg taactttgaa
    17041 ggccgtcaag gcaacggcgg acgtacccac ctcgtcagcc ccgctatggc agcagcagcc
    17101 gccgttaccg gccgctttac cgacatccgc atgatggcgt aagtattttc aggcggtctt
    17161 taagaatttg acgcggccgt ctgaaatatg tcccgtaagg cagaaacacc tttaatattc
    17221 aaaccaagga gtttaaccat gaacaaactt ttcattaccg ccctgtccgc cctcgccttg
    17281 tccgcctgcg ccggcacttg ggagggcgcg aaacaagaca ccgcccgcaa ccttgacaaa
    17341 acacaggccg ccgccgaacg cgccgccgaa caaacaggca acgccgtcga aaaaggctgg
    17401 gacaaaacca aagaagccgt caaaaaaggc ggcaatgccg tcggacgcgg catttcccat
    17461 ctcggcggaa aaatcgaaaa cgccaccgaa taaccgattc gcgatgccgt ctgaaaccgc
    17521 ttcagacggc atcgtcagca caagacgcaa acccatgaaa gcctttacca aaattaccgc
    17581 catcgtcgcc ccgctcgacc gcagcaacgt cgataccgac gccatcatcc ccaaacaatt
    17641 tttaaaatcc atcaaacgca gcggcttcgg ccccaatgcc tttgacgaat ggcgttacct
    17701 cgaccacggc gaaccaggca tggacaacag caaacgcccg ttgaaccctg atttctcgct
    17761 gaaccagccg cgttaccaag gcgcgcaaat cctgttgacg cgtaaaaact tcggttgcgg
    17821 ctcttcacgc gaacacgccc cttgggcatt ggacgactac ggcttccgcg ccgtcatcgc
    17881 ccccagcttc gccgacatct tctttaacaa ctgctacaaa aacggccttt tgcctatcgt
    17941 gttgaccgaa gaacaggttg accggctttt caaagaagtt gaagccaacg aaggctaccg
    18001 gctctccatc gaccttgccg ggcaaaccct gaccaccccc ggcggcgaaa cattagcatt
    18061 cgacattacc gaacaccgca aacactgcct cttaaacggc ttggacgaaa tcggactgac
    18121 cctgcaacac gccgatgaaa ttcacgcctt tgaagaaaaa cgccgccaaa gccagccttg
    18181 gctgtttaac ggttaaacct atgccgtctg aagaccgttc agacggcatt cctgataaca
    18241 tataattgct gcaactaatg taaacagagg ggctataaat tgctttcatc tttcaagcgg
    18301 gcttagcaac gctctaaatt aatgcaatac agctctattt caaataaatt tttagatttt
    18361 taatatgcaa cagattaaat ttattgactt attttccggg atgagcggta tcaggaaagg
    18421 atttgaacaa gcctgtcgga aacagtcggt tgcttgcgaa tgtgttttta cctcagaaat
    18481 caaaccggca gctttggaag tattgaagca aaactaccct gatgaagtgc cgtatggaga
    18541 tataacgaaa attgaaacgg gagatattcc cgattttgac atcttgttgg caggcttccc
    18601 ttgccaggct ttttctttcg ccggaaaaag attgggcttt gaagatacgc ggggaacgct
    18661 tttctttgat gtggcaagga ttttaaaggc aaaaaaacca aaaggtttta ttttggaaaa
    18721 tgtggaagga ttggtgacgc acgatagaaa agatccgaca caaaaaatag gacgcaccct
    18781 gaccgttatt ttggaaacct tggaaacatt gggctactat gtttcttgga aagttttaaa
    18841 tgcaaaagat ttcggcattc cccaaaacag gaagcgcatt tatctgacag gcagtttgaa
    18901 atccaaaccc gacttatctt ttgaaacaac cctaagtccg aaattaaaaa atattttgga
    18961 atcgggactg cctaccgaaa gcagtccttt catcaaaaaa ttgctaaaga aatttccccc
    19021 gtccgaactg tacggaaaat cagtaaagga caaacgggga gggaaaaaca atatccacag
    19081 ttgggatatt gaattaaaag gcacagtaac cgaagaagag aagcaattat taaatatgct
    19141 tctaaaagaa cggaggaaga aaaaatgggc ttcagaaatc ggcatcgatt ggatggatgg
    19201 gatgcctttg acaaaagcgc aaatttcaac tttctataaa caccccaatc ttcaaaatat
    19261 tttggacagc ctgacagata aaggctattt ggttttggag catccgaaac aaaaaattgg
    19321 cgggcgaaga atcaaagatg aatccctgcc caaaggctac aatattgttt caggaaaaaa
    19381 atcatttgaa atcaataaaa tattagaccc aaatgatgtt gcgccaacct tagttgcaat
    19441 ggatatggag caccttttcg tcgttgacaa cggcggtttg cgcacactta ccggaaaaga
    19501 aggtttacgc ttattcggct atccggacga ttattcgttt gatattccta aaaaagacaa
    19561 atacgattta ttgggcaata ccgttgccgt tcctgtgatt aaggcggtat ctgaaaggct
    19621 tctgcatact ttataaaccg atagggataa aatggtaaaa cttactgcac aacaaatatt
    19681 tgataagttg ctgaatgagg aaaaaatctt atcggtcaat ggtcaaatta gatttttctt
    19741 gggcgatgtg gatattatcg tcaaacaaaa agatgtcgtc ggcaacatca ttcaggaatg
    19801 gctcggcgga tggttgagaa aaagggaaat tgaatttgat gtttcaacca atacccaaat
    19861 gccccccgat ttctttttaa ataaaaaaga ccgcagtaga gaattgttgg aagtaaaggc
    19921 gttcaaccga aatgccagtc cgggttttga tattgcagat tttaaaatgt attctgatga
    19981 aatcattcat aaaccctata tgctggatgt agattattta atattcggtt acgatatgga
    20041 cgacaacggc aatgtaacca tcaaggattt atggcttaaa aaagtatggc aaattacgag
    20101 aagcatggat gggtgggcaa ttaatcttca agtcaaaaaa ggcgtggtgc ataaaatccg
    20161 cccgggtgtt tggtacagca taaataaaaa gaatatgcct atgtttgaat gcttggaaga
    20221 ttttgtttcc gcaattgaag aaaccgttta tcaaaacccg gcaacacggc ataacgcatc
    20281 cttatggaaa aagaaatttg aggaagccta taaaaaacac tacaaccgat cgatttccat
    20341 acctcgttgg cacgaaattg cacacaaata caaaaagaaa taacccttca accatcaacc
    20401 cataaggaaa cttgaaatga ccaaacatat cgccatcctc cgaggtgacg gcatcggtcc
    20461 cgaaatcgtc gccgaaaccg tccgcgtact cgacaaactc atcgcccaag gtttggatgc
    20521 cggctacgaa tacgccccat tgggcggcga agcctatgac gaatacggtc atccttatcc
    20581 cgaatttacg caaaacctct gccgcaaagc cgatgccgtc ctgcttggtg cagtcggttc
    20641 tcctcaatac gacaacctcg accgtccgtt gcgccctgag cgcggcctgt tggcaatccg
    20701 taaggatttg aacctgtttg ccaatttgcg tcctgctgtt ttatatcccg aattagccaa
    20761 cgcctccacg ctgaagcctg aaatcgttgc cggtttggat attttgattg tgcgcgaact
    20821 caccggcgac atctatttcg gcgaaccgcg cggcatccgc gttctggaaa acggcgaacg
    20881 cgaaggctac aacaccatga aatacagcga aagcgaaatc cgccgtattg cccatgttgc
    20941 cttccaatcc gcccaaaaac gcagcaaaaa agtctgttcc gtaggcaaag ccaacgtttt
    21001 ggaaaccacc gaattgtggc gcgaaatctt tgaagaaatc ggcaaagagt accctgatgt
    21061 cgagctttcc catatgtacg tcgacaacgc cgccatgcag ctcgtgcgtg cgcccaaaca
    21121 attcgacgtc attgccaccg gcaacatctt cggcgacatt ctctccgacg aagcctccat
    21181 gctgaccggc tccatcggta tgctgccttc cgcctcgctg gacgaaaacg gcaaaggcct
    21241 gtacgaaccg tctcacggct ccgcccccga catcgccgga caaaacaaag ccaacccgct
    21301 ggccaccatc ctctcgctcg caatgttgct gcgttacagc ctgaacgacg aagcgcgcgc
    21361 gcaacaagtt gaaaacgccg tccaaaaagt gctgcaacaa ggcttgcgta ccagcgacat
    21421 ttacgaagaa ggcacaaaac tcgtttcctg ctccgaaatg ggcgatgcgg tactcgccgc
    21481 cttgtaaaaa aacaaagcgc ggatcacacc gcgctttttc atccgccctt ttccgcccgc
    21541 cccgttcgtc aaccgcctgc aaacccaccg tttaccggca ggaaatgcca taaatccctg
    21601 cctatgttag gataaacgcc tttaccggcc cacacaaagg attatcatca tgaaaaaaat
    21661 catcatcgcc gcgctcgcag ccgccgccat cggcactacc tccgccgcca cctacaaagt
    21721 ggacgaatat cacgccaacg cccgtttcgc catcgaccat ttcaatacca gcaccaacgt
    21781 cggcggtttt tacggtctga ccggttccgt tgagttcgac caagcaaaac gcgacggtaa
    21841 aatcgacatc accattcccg ttgccaacct gcaaagcggt tcgcaacact ttaccgacca
    21901 cctgaaatcc gccgacatct tcgatgccgc ccaatatcct aacatccgct tcgtttccac
    21961 caaattcaac ttcaacggca aaaaactggt ttccgttgac ggcaacctga ccatgcacgg
    22021 caaaaccgcc cccgtcaaac tcaaagccga aaaattcaac tgctaccaaa gcccgatgtt
    22081 gaaaaccgaa gtttgcggcg gcgacttcag caccaccatc gaccgcacca aatggggcat
    22141 ggactacctc gttaacgttg gtatgaccaa aagcgtccgc atcgacatcc aaatcgaggc
    22201 agccaaacaa taaatcttga tgcgaatcag cacaaatgcc gtcttcatca aaccgccccg
    22261 ccaacggtaa gccgatatgg cggagcacaa tccgccgaaa gatatttcag cggcaggcag
    22321 ggcttgccgg aaagtccgga aa
//
LOCUS       DAXHPG010000015        21716 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXHPG010000015 DAXHPG010000000
VERSION     DAXHPG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.015"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(92..784)
                     /locus_tag="ACFM6F_000418"
                     /pseudo
     CDS             complement(92..784)
                     /locus_tag="ACFM6F_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1043..1858)
                     /locus_tag="ACFM6F_000419"
     CDS             complement(1043..1858)
                     /locus_tag="ACFM6F_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163898.1"
                     /translation="MASIALYRLSFPVAAGGSGSRCTLCTKVHRESMLAIYSINILIY
                     NEKMFFRVTLYFVKNFVSFSKTRKSNEMMQKHKCLIDFILTVASQIAYADLPLSLEEL
                     LTDKGKFKLESSISYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQV
                     FDSQNVKAIQLSQAEMKETQGEFVPIIAAAAFGGALGTWGYHGANLYNHGKLGTAQGA
                     ATAAGIGAATGVAATGLAAAAGGGLAGNLAWRPGIHALGFGANAANNAVNSRK"
     gene            complement(1889..2047)
                     /locus_tag="ACFM6F_000420"
     CDS             complement(1889..2047)
                     /locus_tag="ACFM6F_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163899.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2044..2886)
                     /locus_tag="ACFM6F_000421"
     CDS             complement(2044..2886)
                     /locus_tag="ACFM6F_000421"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6163900.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPTDERVLLAQAIEEKHAAEKAQEQTVSGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            2952..3092
                     /locus_tag="ACFM6F_000422"
     CDS             2952..3092
                     /locus_tag="ACFM6F_000422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163901.1"
                     /translation="MPSEKYGIRAVKLCGGKCAIIRAVLPLLRSCSANTRAAWTYNRM
                     PA"
     gene            3122..4042
                     /gene="pyrB"
                     /locus_tag="ACFM6F_000423"
     CDS             3122..4042
                     /gene="pyrB"
                     /locus_tag="ACFM6F_000423"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC6163902.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4052..4510
                     /gene="pyrI"
                     /locus_tag="ACFM6F_000424"
     CDS             4052..4510
                     /gene="pyrI"
                     /locus_tag="ACFM6F_000424"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC6163903.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4575..5240
                     /locus_tag="ACFM6F_000425"
     CDS             4575..5240
                     /locus_tag="ACFM6F_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163904.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5303..6520)
                     /locus_tag="ACFM6F_000426"
     CDS             complement(5303..6520)
                     /locus_tag="ACFM6F_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6163905.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWSRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAGE
                     RLKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6704..7207
                     /gene="def"
                     /locus_tag="ACFM6F_000427"
     CDS             6704..7207
                     /gene="def"
                     /locus_tag="ACFM6F_000427"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC6163906.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLIADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDTVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            7294..8220
                     /gene="fmt"
                     /locus_tag="ACFM6F_000428"
     CDS             7294..8220
                     /gene="fmt"
                     /locus_tag="ACFM6F_000428"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC6163907.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDVPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDAGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLRKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8299..9558
                     /gene="rsmB"
                     /locus_tag="ACFM6F_000429"
     CDS             8299..9558
                     /gene="rsmB"
                     /locus_tag="ACFM6F_000429"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC6163908.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAILRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIPARALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9524..10123
                     /locus_tag="ACFM6F_000430"
     CDS             9524..10123
                     /locus_tag="ACFM6F_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC6163909.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10123..12240
                     /locus_tag="ACFM6F_000431"
     CDS             10123..12240
                     /locus_tag="ACFM6F_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6163910.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLISTASLSGNMGRVLEHY
                     ANGFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAEQ
                     ILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVKTYA"
     gene            12233..13510
                     /locus_tag="ACFM6F_000432"
     CDS             12233..13510
                     /locus_tag="ACFM6F_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC6163911.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QVRPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKHGAAKIETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13601..14788
                     /gene="dprA"
                     /locus_tag="ACFM6F_000433"
     CDS             13601..14788
                     /gene="dprA"
                     /locus_tag="ACFM6F_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC6163912.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRASARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGSRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14814..15275
                     /locus_tag="ACFM6F_000434"
     CDS             14814..15275
                     /locus_tag="ACFM6F_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC6163913.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15347..17653
                     /gene="topA"
                     /locus_tag="ACFM6F_000435"
     CDS             15347..17653
                     /gene="topA"
                     /locus_tag="ACFM6F_000435"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC6163914.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAETCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17731..18000)
                     /locus_tag="ACFM6F_000436"
     CDS             complement(17731..18000)
                     /locus_tag="ACFM6F_000436"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFC6163915.1"
                     /translation="MIASSEPFPCSACGLCCQKVNLSEQTIYLDRGDGTCRHFDDSTR
                     LCTIYETRPLVCRVEDYYHHYLSEQMSWQDFIQINIEVCKQFQSE"
     gene            complement(17997..18416)
                     /locus_tag="ACFM6F_000437"
     CDS             complement(17997..18416)
                     /locus_tag="ACFM6F_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163916.1"
                     /translation="MGKIQILNKLPTKNPATIPFDAASNSFFRILEARNEYKKIAAQE
                     KTKREVIGAWRDVRLAELKNQQEILRLYLENTFKERRKMIDGLFEALDKGMEAGNIHV
                     IDTAINGIVSIAKDSPLKQVDNLIAALKNDNVKVIDF"
     gene            complement(18416..19363)
                     /locus_tag="ACFM6F_000438"
     CDS             complement(18416..19363)
                     /locus_tag="ACFM6F_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163917.1"
                     /translation="MAIPVLLWGAAAALAATGIVKGAAALSDIDDAKEIGERAERRYK
                     RRKRELNSTKDETNLAFEELGQFKVDIFNNQIRHIVQVLNNTSNKHAKSELSGFNSTF
                     DIAELKEMETMVQTSLELSTNLGSSAITGALAGFGVYGAVPMIATASTGTAISTLSGA
                     AATNATLAWLGGGSLAAGGFGMAGGMVALGGIVAGPALAIGGFMLASKAEEALTEARA
                     YSAEIDEAIDEMDLITEGLEGLKENAREIRHTLEEMVKRFEKVRVDHADHPDFHTMLV
                     IGKAIKNLLNTPIMEEDGSPVANIRHQCEGCLNIELGTV"
     gene            19520..19897
                     /locus_tag="ACFM6F_000439"
     CDS             19520..19897
                     /locus_tag="ACFM6F_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFC6163918.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19986..20555
                     /gene="rsmD"
                     /locus_tag="ACFM6F_000440"
     CDS             19986..20555
                     /gene="rsmD"
                     /locus_tag="ACFM6F_000440"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC6163919.1"
                     /translation="MAAGKHTKHSNQVRIIGGQCRGRKLSFASANGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGRL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20678..20929
                     /locus_tag="ACFM6F_000441"
     CDS             20678..20929
                     /locus_tag="ACFM6F_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC6163920.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            21026..21109
                     /locus_tag="ACFM6F_000442"
     tRNA            21026..21109
                     /locus_tag="ACFM6F_000442"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21060..21062,aa:Tyr,seq:gta)
     gene            21140..21213
                     /locus_tag="ACFM6F_000443"
     tRNA            21140..21213
                     /locus_tag="ACFM6F_000443"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21172..21174,aa:Gly,seq:tcc)
     gene            21223..21297
                     /locus_tag="ACFM6F_000444"
     tRNA            21223..21297
                     /locus_tag="ACFM6F_000444"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21255..21257,aa:Thr,seq:ggt)
     gene            21346..>21716
                     /locus_tag="ACFM6F_000445"
     CDS             21346..>21716
                     /locus_tag="ACFM6F_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6163921.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLAR"
BASE COUNT         5415 a         5588 c         5857 g         4856 t
ORIGIN      
        1 aagtgaatgg gtttcgtact atttgtactg tctgcggctt cgtcgccttg tcctgattac
       61 tatataaaat aaatgggcaa aaatcggttt attatcgttt ttgccgcatt tggatttgtt
      121 ctaccgtaaa acgtgtttga cgaacgggat tcttattaaa aaacatctga tttctaacaa
      181 aatcagtatt ttttggcacg atggctaaaa tttttccttc catttcgcca tcacgtgttt
      241 tccatgcgct caagaattgt gatttgctca ttgagacgtg cccccagcga tggatcagcc
      301 agcaaaacag tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat
      361 tttaaataca caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa
      421 ccttgtgctt caaaacccag ttcgggcatt atgcgttgca tatcatcaaa agaagcacgc
      481 atttgggttt tatccatttt gtctaagatt tccgcttcag aataatgtct gccataaaaa
      541 ttattcagta acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact
      601 atgccggaat ctcgccgtgc tttccaactt cgtacatgga tgttttggta agaagcgggg
      661 ggtcaacaaa cataggccaa gcaaaaacta tatttggggc gaaaccaatc aaagccgcat
      721 aatttatcaa tttataaaga ttttttatca taatatgtat acgcggaata aacgcatgaa
      781 acataaaaaa ataaaatcat atgcaatttt attgcatgaa taaatatgaa taaaatagat
      841 aatgatggga gtaaatacgc catgtatttt ggaagtttaa atttattaat aataaaataa
      901 tttatcgtag cgcaaataaa tcccaaaatt ggaacgatta gaaaaaaaat taatacaccc
      961 atcatcatgc aaactctata ttaagaaata gctaactaat gctattgtgg aaattagtta
     1021 gctaactaaa agttattaat gattattttc gagaattgac tgcattatta gcagcattgg
     1081 caccaaaacc tagtgcatga atacccggtc tccatgccaa attcccagcc aatccgcctc
     1141 cggcagcagc agccagccct gttgccgcta ctcctgttgc cgcaccgatt cctgccgcag
     1201 tagccgcgcc ttgcgcagtt cctaatttac catgattata caaattagca ccatgatacc
     1261 cccatgtacc taatgcaccg ccaaaagcag cggctgcaat aatgggaaca aattcacctt
     1321 gtgtttcttt catttcagcc tgtgataatt gaattgcttt cacattttgg ctgtcaaaaa
     1381 cttggctgtc taaattttgc gccattacag gtgtaatcat catagccatt acagttgcaa
     1441 ttttcgttgc gctggtttgc acataaatag gattagcaaa ttcgctttga ttgcgttcag
     1501 tgttgatgta gctaatactg ctttctagtt tgaatttacc cttgtcagtc aataattctt
     1561 ccaaacttaa aggtaagtca gcataagcaa tttggctggc aacagtcaaa ataaaatcta
     1621 ttagacattt gtgtttttgc atcatttcgt ttgattttct agtttttgag aatgatacaa
     1681 agttttttac aaagtaaaga gtcactctga aaaacatttt ttcattataa atcaaaatat
     1741 tgatagaata aatagcgagc atcgattcac ggtgtacttt agtgcaaagc gtacaacgcg
     1801 agcctgaacc accagccgca acaggaaaag aaagccgata gagtgcaatg cttgccaacg
     1861 tgcaagcgag cttgcaagaa cgcttggctc aacgagagca ggcaagacag aaagcagaaa
     1921 gcaggatagg agcggtaacg caaaggtctc gggctttgat ttcgccgtaa accctgctgc
     1981 cgccttgtcc ggaaagggtg caggcggcga gtgccgacag ggtgcagatg gggagggggg
     2041 ttttcatttg gggtcgcaac ggaagtggta tgcgcagatt tcaaaaccgt ttttgaaata
     2101 caggcggtgc gcgtcggcac ggtcgtggtt gacgtggacg ttgaggtgga ttttggttac
     2161 ccctgtttcc gcgccgattt tgcggacttc ttccaaaagg cgcgaggcgt agcctttgcg
     2221 gcggctttgc ggcagggtaa cgatgtcatc gatgtggatg tggcggccgc cggcgagggt
     2281 gcaggcttcg cggaagccgc agacggcgac ggcgttgtgt ttgccttctt caaaaatgcc
     2341 cagcaggcgg tagccttggg ggcgttggac tttgttgatc tgttcggtaa agcggttgat
     2401 gtcggtcagg gcggaacgca aaacgctcaa ggctgcaaag gcggtggcgg tgtcgtccgc
     2461 gccgatttcg cgcaaaacgt aggatgcgcc cgagacggtc tgttcctgtg ctttctcggc
     2521 ggcgtgtttt tcttcgattg cctgtgccag caggacgcgc tcgtcggtag ggtcgttttg
     2581 tccgccctgt tcgcgttctt cgagcagggc tttgcagtcg atgacgcgca ggtcgttgtc
     2641 ggcggcaaag tccatcagga agcggaacat ctgggggttg tcggtttcca gttttttatc
     2701 gacggcgacg cagcggatgt tgtccaccag catggggcgc acccatttcg agtaggaaag
     2761 cctgttgtct ttggtaaacg aggacagaat gccgcacagg cgttccgccc agtcgctggg
     2821 acggaaaatc ttgccggaac tcgttgtgcc gtggatgacg acttcgtagg ggttgcagac
     2881 taacatggcg gcttcctgaa aagaaatgtc tggcgcgatt atacctgatg cttatgcggg
     2941 cgtgtttgga tatgccgtct gaaaagtacg ggattcgtgc ggtaaaactt tgcggcggca
     3001 aatgtgcgat aatacgcgcc gtattgccgc ttttgcgaag ctgttccgca aacactcggg
     3061 cggcgtggac gtataaccgg atgcccgcct gacgcgggtt ttttacggaa ggggggcaaa
     3121 aatgcctaat ccgctttaca gacagcacat catctccatt tcggatttgt cgcgcgaaca
     3181 gttggaatgc ctgcttcaga cggcattgaa gctgaaggcg catccgcgcg gcgatttgtt
     3241 ggaaggcaaa cttatcggtt cgtgcttttt cgagccgtcc acgcgcacga ggctgtcgtt
     3301 tgaaacggcg gtgcagcggc tgggcggcaa ggtcatcggc ttttcggacg gcgcgaatac
     3361 cagtgccaaa aaaggcgaga cgcttgccga taccgcccgc atcatttccg gctacaccga
     3421 cgcaatcatc caacgccacc ccaaagacgg cgcggcgcgc gtggcggcgg agttttcgcg
     3481 cgtccccgtc atcaacgccg gcgacggcac gaaccagcat cccagccaga ctttgttgga
     3541 tctggtaacg atttatgaaa cgcaggggcg tttggacaag ctcaaaatcg ccatggcggg
     3601 cgacttgaaa tacgggcgca ccgtgcattc gctttgtcag gcgttgaaac gctggggctg
     3661 cgaatttgcc tttgtttcgc cgcccagcct cgcgatgccc gactacatta ccgaagagtt
     3721 ggacgaagcc ggctgccgat accgtatcct cggcagtttg gaagaggcgg cggaatgggc
     3781 ggacatcctg tatatgaccc gcgtccagcg cgaacgtttc gatgaacagg aatttgccaa
     3841 aatccaaggc aaattcaacc tcgaagcgtc tatgctcgcc cacgccaaac cgaacctgcg
     3901 cgtgctgcac cctttgccgc gcgtggacga aatccatccc gatgtcgatg ccacgccgca
     3961 tgcctattat ttcgagcagg caaccaacgg cgtttatgcg cgtatggcga tattgtcgct
     4021 ggtgttgaac gaagaagtgt gaggaaccga tatggaaacc ccgaaactca gtgtcgaagc
     4081 cattgaaaaa ggtacggtta tcgaccatat tcccgctggc agggggctga ccatcctgcg
     4141 ccagttcaaa cttttgcact acggcaacgc agtaaccgtg ggcttcaacc tgcccagcaa
     4201 aacccaaggc agcaaagaca tcatcaaaat caaaggcgtg tgcttggacg acaaagccgc
     4261 cgaccgcctt gccctgtttg cacccgaagc agtggtcaac accatcgaca atttcaaggt
     4321 tgtgcagaaa cggcatttga acctgcccga cgaaatcgcc gaagtgttcc gctgtccgaa
     4381 cacgaattgc gccggccacg gcgagccggt caaaagccgg ttttatgtta aaaaacacaa
     4441 cgggcagacg cggctgaaat gccattactg cgaaaaaacc tacagccggg attcggtggc
     4501 ggaagcctga cggattccct taaaccgagc gggcggcatt tcgtctgccg cttgttttgc
     4561 caatctgaaa tggaatgatg atgcacgctt ctgtccaaag ccgtttcgca ccgatacttt
     4621 atgttttgat tttctttgcc ggttttttga ccgcgcaaat ctggttcaat cagaaagcct
     4681 atactgaaga gctgcctccg cttctgtccg cattgtccgc tgtcgcgctg gtgtggctgg
     4741 cgtgggcgtt cgtgtcggcg cgttcaaagg ctaaggcgga aaagttctac cgcgaaaaaa
     4801 tgatacagaa cgaaagcata caccccgtcc tgcacgcttc tttgcaacac ttggaacaca
     4861 agccgcaaat gctcgccctg ctggtcaaaa accacggcaa agggatggcg gaacaggtca
     4921 ggttcaaggc ggaagtgctg cccgacgacg aagacgcgcg cacgattgcc gccgagttgg
     4981 caaaaatgga tatgtttgca ttggggacgg acgcggtcgc ctcgggcgaa acctatggac
     5041 gcgtgttcgc cgatattttc gagttgtcgg cggctttgga agggcgcgcg ttcaaaggaa
     5101 tgttgaaact gacggcggaa tataaaaaca tcttcggcga tgcctgccgt tcggaaacgg
     5161 cgttggagtt gggcgcgctc aatcaggcgt tgcaggagat ttcaaaaaca tcggaaaagt
     5221 ccaaacggat attttattga agatggaaaa atgccgtctg aaacggaagg tgtttcagac
     5281 ggcatttttg tcggatgatt aattattcgg agcggttgaa gccaaacttc acgcggctgc
     5341 ggccctgatc cggtatattg tccaaatcgc gtcccggatt ggcggcggtg tcgcctacgg
     5401 aaatatcgga gatgttttcc aaaatgatgg cggacgacag gtgttcggag gtgcggtaaa
     5461 ccattgccaa gcccacttct tcggcaggag tggaaatcag ctcgacggta tccctgcttt
     5521 tgaaattgtt ggagaggtcg acctgcatcg ttttcttgcg tttgtagagg ctcaaaaccg
     5581 tgcctttgtc caaaccgtcc gcctcgcctt tgtcgatggt gatggtttga aactggccgg
     5641 caatccttgt gccttcaaac acggaaacga ttttagcctg aaccgggcgg gacggttcgt
     5701 gcggcatcat gttgaagcgg tcggtgtctt ccggcatttt catcaggtag tcgccctgct
     5761 gtatttcgga aatggcggtt tcgaccacca gcggctgtat cgaaggggtg cgcagcgggg
     5821 taatcaaagg atgggtgcgg gtatggtatt cgttgtcttt cagccgttct ccagcctgtt
     5881 tcgagcgttg ttcgagggcg gagtcggtat agtcgaggga gcgcacgatg ccgctgaatg
     5941 cgacttcctg cccgaggaat ttacccgtat ccggatcggt gatgttttta ttgattcggt
     6001 aggtcaggta gcggcccggc tctttcaggc ctttggtgta aaccctggtg cctttggtgt
     6061 acagcagcct gccttccggg cccgagagca ggcgcggcgc ggcagcggtt tctttgcggg
     6121 aaacgatttg cggatgccgc ataaagatgc ggtagaagtt gacatcgatg gcgggaatac
     6181 cgtatccgga cacttcctta tccggactca ttttgacgac ggggatgccg tctgtctgtt
     6241 ccaagccgag gcgcggttcg ccgtcaacgt ggcgcaacac caatacctgg tccggataaa
     6301 tcaggtcggg attgtggatt tgatcccggt tcgcgtccca caggcggctc cattgccacg
     6361 ggctgtacag gtatttgccc gaaatgcccc acagggtgtc gccctgtttg accgtgtagc
     6421 gttccggcgc gttcgggcgc acctccaaat ttgccgccaa agtttgtgtt gagaatgcca
     6481 tacctgccgc gcagagcagg gttataatac gacgttgcat aaccgttccc cttatctgat
     6541 aaatttcggt ttgtcttgct tgattgggtt ggaaaaagcg gcggcagccc ctcgggatgt
     6601 gccgcgtgat aaaaaatgtt ccgcatttta acatcgaatt atccgcacca tcacggtaat
     6661 tatgaaaaac aggcggcgta tccgccgaag gaaagagaaa attatggctt tattgaatat
     6721 cttgcaatat cccgacgagc gtctgcacac ggtggcaaag cctgtcgaac aagtcgacga
     6781 gcgcatccgg aagctgattg ccgatatgtt tgaaacgatg tacgaatcgc gcggcatcgg
     6841 gctggcggcg acgcaggtcg atgtgcacga gcgcgtggtc gtgatggatt tgaccgaaga
     6901 ccgcagcgaa ccgcgcgtgt tcatcaaccc cgtcatcgtt gaaaaagacg gcgaaaccac
     6961 ttacgaagag ggctgcctgt ccgtgccggg catttacgac accgtaaccc gcgccgaacg
     7021 cgtcaaggtc gaggctttga acgaaaaagg cgaaaagttc acgctggagg cggacggctt
     7081 gctggcgatt tgcgtgcagc acgagttgga ccacctgatg ggcatcgtgt ttgtcgaacg
     7141 cctgtcgcag ctcaagcagg gacgcatcaa aaccaagctg aaaaaacgtc agaaacatac
     7201 gatttgaccc ttttgccgtg ccgtctgaac gctgcaaagt tttcagacgg cacggtcttg
     7261 tccgacaacc ttacgcatac gcaggaatat gctatgaaag tcatctttgc cggcacgccc
     7321 gattttgccg ccgccgcctt aaaagccgtt gccgccgccg gttttgaaat cccgctggtg
     7381 ctgacccagc ccgaccgtcc gaaagggcgc ggtatgcaac tgaccgcccc gcccgtcaaa
     7441 caagccgcgc tggaactcgg tttgcgcgtg gcacagcccg aaaaactgcg taacaacgcc
     7501 gaagccctgc aaatgctcaa agaggtcgag gcagacgtaa tggtggttgc cgcctacggt
     7561 ttgattctgc cgcaagacgt gttggatgtg ccgaaacacg gctgcctcaa catccacgct
     7621 tcgctgctgc cccgttggcg cggcgcggcg ccgattcagc gcgcgattga ggccggcgat
     7681 gccgaaaccg gcgtgtgcat tatgcagatg gatgccggtt tggacaccgg tgatgtggtc
     7741 agcgaacacc gctacgccat ccaaccgacc gataccgcca acgaagtcca cgacgcgctg
     7801 atggaaatcg gtgcggcggc ggttgttgcc gatttgcaac agcttcaaag caaaggccgt
     7861 ctgaacgcgg tcaaacagcc cgaagaaggt gttacttacg cgcaaaaatt gcgcaaagaa
     7921 gaggcgcgta tcgattggag cgaaagcgcg gcggttatcg aacgcaaaat ccgcgctttc
     7981 aaccccgtgc ctgccgcgtg ggttgagtat cagggcaagc cgatgaaaat ccggcgggcg
     8041 gaagtggtgg cgcaacaagg cgcggcaggc gaagtgttgt cctgttcggc ggacggtttg
     8101 gtcgttgcct gcggcgaaaa cgcgctgaag attaccgaat tgcagcctgc cggcggcagg
     8161 cgtatgaata tcgcggcgtt tgcagcagga cggcatatcg aagcagggac gaagctgtaa
     8221 atcccttcag acggcattcc gatccgcaaa cgggaatgcc gcctgaaacc atcagttgaa
     8281 gaaagcgaat cacataatat gagtatggca cttgcccaaa aacttgccgc cgacagcatt
     8341 gcggcggttg ccgaaggacg caaccttcag gacgtgttgg cgcaaatccg caccgcgcat
     8401 cccgacctta cggcacagga aaacggcgcg ttgcaggaca tcgcctacgg ctgccagcgt
     8461 tatttgggca gtttgaaaca tatgctcgcg cagatgctga aaaagccgat tgacaacccg
     8521 cagctcgaaa gcctgctttt ggcggcgttg taccaactgc attacacgcg caacgcgccg
     8581 cacgccgtgg tcaacgaagc ggtggaaagc atagccaaaa tcggacgcgg gcagtaccgt
     8641 tcgtttgcca acgcgatttt gcgccgcttt ttgcgcgaac gcgacaagct tgcggcttcc
     8701 tgtaaaaaag acgatgtggc gaaacacaac ctgccgctgt ggtgggtggc ttacctgaaa
     8761 aaccattatc cgaaacactg gcacaacatc accgccgcgc tacaatccca tccgccgatg
     8821 acgttgcgcg tcaaccgccg acacggcaat gccgaaagct atttggaaaa actggctgcg
     8881 gaaggtattc cggccagggc gttggacgaa tatgcggtta cgttggaaga agccgtgccg
     8941 gtaaaccgtc tgcccggttt ttcagacggc attgtttcgg tacaggactt cggtgcgcag
     9001 caggcggcgt atcttcttaa cccgaaagac ggcgaacgga ttttggacgc gtgcgccgcg
     9061 ccgggcggca agacggggca tatcttggaa ctggcggatt gccgcgttac cgccttggac
     9121 atcgatgcag gccgtctgaa gcgtgtggag gacaatatcg cccgtttggg ctttcagacg
     9181 gcatcggcgg cgtgtgccga tgcgcgggat ttggcggcgt ggtatgatgg aaagcctttc
     9241 gacgcaatcc ttgccgacgt gccatgcacc gcttccggcg tggcgcggcg caatcccgac
     9301 gtgaaatggc tgcgccgtcc gaccgacgcg ctcaaaaccg cccgccagca ggaagccctg
     9361 ctggacgcac tttggcagac gctgaaacag ggcggcagaa tgctgcttgc cacctgttcc
     9421 gtgttcgtcg aagaaaacga cgggcaattg caaaaattcc tcaaccgcca tgccgatgcc
     9481 gaaccgatcg aatcgcgggt gcttttaccg aacaaacacc aagatggctt ttattacgcg
     9541 cttattcaaa agcattaaac aatggcttgt gctgctgccg atgctttccg ttttgccgga
     9601 cgcggcggcg gaggggatag atgtgagccg cgccgaagcg aggataaccg acggcgggca
     9661 gctttccatc agcagccgct tccaaaccga gctgcccgac cagctccaac aggcgttgcg
     9721 ccggggcgtg ccgctcaact ttaccttaag ctggcagctt tccgccccga taatcgcttc
     9781 ttatcggttt aaattggggc aactgattgg cgatgacgac aatattgact acaaactgag
     9841 tttccatccg ctgaccaacc gctaccgcgt taccgtcggc gcgttttcga cagactacga
     9901 caccttggat gcggcattgc gcgcgaccgg cgcggttgcc aactggaaag tcctgaacaa
     9961 aggcgcgctg tccggtgcgg aagcagggga aaccaaggcg gaaatccgcc tgacgctgtc
    10021 cacttcaaaa ctgcccaagc catttcaaat caatgcattg acttctcaaa actggcattt
    10081 ggattcgggt tggaaacctc taaacatcat cgggaacaaa taatgcgccg ttttctaccg
    10141 atcgcagcca tatgcgccgt cgtcctgttg tacggactga cggcggcaac cggcagcacc
    10201 agttcgctgg cggattattt ctggtggata gttgcgttca gcgcaatgct gctgctggtg
    10261 ttgtccgccg ttttggcacg ttatgtcata ttgctgttga aagacaggcg cgacggcgta
    10321 ttcggttcgc agattgccaa acgcctttct gggatgttta cgctggttgc cgtactgccc
    10381 ggcgtgtttc tgttcggcgt ttccgcacag ttcatcaacg gtacgattaa ttcgtggttc
    10441 ggcaacgata cccacgaggc gcttgaacgc agcctcaatt tgagcaagtc cgcattgaat
    10501 ctggcggcag acaacgccct tggcaacgcc atccccgtgc agatagacct catcagcacc
    10561 gcctccctat ccggcaatat gggcagggtg ctggaacatt atgccaacgg gtttacacag
    10621 ttgtcccttt acaatgccgc aagcggcaaa atcgaaaaaa gcatcaaccc gcacaagctc
    10681 gatcagccgt ttccggataa ggcgcgttgg gaaaaaatcc aacaggcggg ttcggtcagg
    10741 gatttggaaa gcataggcgg cgtattgtac gcgcagggct ggctgtcggc gggtacgcac
    10801 aacgggcgcg attacgcctt gtttttccgt cagccggttc ccaaaggcgt ggcagaggat
    10861 gccgtcttaa tcgaaaaggc aagggcgaaa tatgctgagt tgagttacag caaaaaaggt
    10921 ttgcagacct ttttcctggc aaccctgctg attgcctcgc tgctgtcgat ttttcttgca
    10981 ctggtcatgg cactgtattt cgcccgccgt ttcgtcgaac ccgtcctatc gcttgccgag
    11041 ggggcgaagg cggtggcgca aggcgatttc agccaaaccc gtcctgtttt acgcaatgac
    11101 gagttcgggc gtttgaccaa gttgttcaac cacatgaccg agcagctttc catcgccaaa
    11161 gaagcagacg agcgcaaccg ccggcgcgag gaagccgcca gacattatct tgaatgcgtg
    11221 ttggaggggc tgaccacggg cgtggtggtg tttgacgaac aaggctgtct gaaaaccttc
    11281 aacaaagcgg cggaacagat tttggggatg ccgcttaccc ccctgtgggg cagcagccgg
    11341 cacggttggc acggcgtttc ggcgcagcag tccctgcttg ccgaagtgtt tgccgccatc
    11401 ggcgcagcgg caggtacgga caaaccggtc catgtgaaat atgccgcgcc ggacgatgcc
    11461 aaaatcctgc tgggcaaggc aaccgtcctg cccgaagaca acggcaacgg cgtggtaatg
    11521 gtgattgacg acatcaccgt tttgatacac gcgcaaaaag aagccgcgtg gggcgaagtg
    11581 gcaaaacggc tggcacacga aatccgcaat ccgctcacgc ccatccagct ttccgccgaa
    11641 cgtctggcgt ggaaattggg cgggaagctg gatgagcagg atgcacaaat cctgacgcgt
    11701 tcgaccgaca ccatcgtcaa acaggtggcg gcattgaagg aaatggtcga agcattccgc
    11761 aattatgcgc gttccccttc gctcaaattg gaaaatcagg atttgaacgc cttaatcggc
    11821 gatgtgttgg cattgtatga agccggtccg tgccggtttg cggcggagct tgccggcgaa
    11881 ccgctgatgg tggcggcgga tacgaccgcc atgcggcagg tgctgcacaa tattttcaaa
    11941 aatgccgccg aagcggcgga agaagccgat gtacccgaag tcagggtaaa atcggaagcg
    12001 gggcaggacg gtcggattgt cctgacggtt tgcgacaacg gcaaagggtt cggcagggaa
    12061 atgctgcaca acgccttcga gccgtatgta acggacaaac cggcgggaac gggattgggt
    12121 ctgcctgtgg tgaaaaaaat cattgaagaa cacggtggcc gcatcagcct gagcaatcag
    12181 gatgcgggtg gggcgtgtgt cagaatcatc ttgccaaaaa cggtaaaaac ttatgcgtag
    12241 cagcgatatt ttaattgtag acgacgaaat cggcatccgc gacctgctgt cggaaatcct
    12301 gcaggacgaa ggttattcgg tcgcattggc ggaaaacgcc gaagaggcgc gcaagctgcg
    12361 ccatcaggta cgcccggcga tggtgttgct ggatatttgg atgcccgatt gcgacggcat
    12421 cacccttttg aaggagtggg cgaaaaacgg gcagctcaat atgccggtgg tgatgatgag
    12481 cgggcatgcc agcatcgata ccgccgtaga agcgaccaaa atcggcgcgc tcgacttttt
    12541 ggaaaaaccg atttccctgc aaaagctgct gtctgccgtc gaaaacgcgt tgaaacacgg
    12601 tgcggcgaaa atcgaaacgg gaccggtgtt cgacaagctg ggcaacagtg cggcgattca
    12661 ggaaatgaac cgtgaggtag gggctgcggt gaaacgtgcc tctcccgtac ttttgacggg
    12721 cgaggcgggt tcgccgtttg aaacggtggc acgctatttc cataaaaacg gtacgccgtg
    12781 ggtcagcccg gcaagggtcg aatatctgat cgatatgccg atggaactgt tgcagaaggc
    12841 ggagggcggc gttttgtatg tcggcgacat cgcccaatac agccgcaaca tccaagccgg
    12901 cattgccttt attgtcggca aggcagaacg ctgccgcgtc agggtggtcg catcgggcag
    12961 cagggcggca ggttcagacg gcattgcctg cgaggaaaag ctggcggaac tgctgtcgga
    13021 atcggtcgtc cgtattccgc cgctgcgtat gcagcatgaa gatattccct tcctgataca
    13081 ggggattgcc tgcaatgtgg cggaaagtca aaagattgcg cccgccgcat tcagtgaaga
    13141 ggcacttgcc gcattgaccc gttacgactg gccgggaaat ttcgaccaac tgcaaagcgt
    13201 cgttgcaacg ctgttgttgg aggcggacgg acaggaaatc ggcgcggcgg cggtttcttc
    13261 ccttttgggg cagaatgtgc ctgccgaggg agcggaagat atggtgggcg ggtttaattt
    13321 caacctgccc ctgcgcgaat tgagggagga ggtggagcgg cgttatttcg agtaccacat
    13381 cgcccaagaa ggtcagaata tgagcaaagt ggcgcagaag gtcggtttgg aacgcacgca
    13441 cctttaccgc aaactcaaac agctcggcat cggcgtttcg cgccgggcgg gggaaaaaac
    13501 cgaagaatag gcccggacgg tcggttttcc ggctgcgggc ttttgttttc agacggcatt
    13561 tggtgcaaat gccgtctgaa atcgtaaggg gacggatttt atgacagagg acgaacgttt
    13621 cgcgtggctg caattggcgt ttacgcccta tatcggcgcg gaaagcttcc tgctgctgct
    13681 gcgccgtttc ggcagcgcgt caaacgccct gtccgcaccg gcggaacagg tcgccccggc
    13741 ggtgcggcac aagcaggcgg ccgaggcttg gcgcaatgcg gaaaaacgcg cctcggcgcg
    13801 gcaggcggca gaagcggcgt tggaatggga aatgcgggac ggatgccgtc tgatgctgct
    13861 tcaggatgaa gatttccccg aaatgctgac gcaggggctg accgcgccgc cggttttgtt
    13921 tttgcgcggc aacgtgcggc tgctgcacaa gccttccgcc gccatcgtcg gcagccgcca
    13981 tgccacgccg caggcgatgc ggattgccaa agatttcggc aagtcgttgg gcgggcaaaa
    14041 tatccccgtt gtgtcgggta tggcttccgg tatcgatact gccgcccatc agggcgcact
    14101 ggaggcagaa ggcggcacca tcgccgtgtg ggggacgggt atagaccgca tttacccgcc
    14161 gtccaataaa aaccttgcct atgaaatcgc cgaaaaagga ttgattgtca gcgagtttcc
    14221 catcggtacg cgcccgtatg ccggcaactt tccgcgccgc aaccgcctga ttgccgcgct
    14281 gtcgcaggtt acgctggtgg ttgaagctgc gttggaatcc ggttcgctga ttaccgccgg
    14341 cttggcggca gaaatggggc gcgaagtgat ggcggtaccc ggttcgatag acaatccgca
    14401 cagcaaaggc tgccacaaac tgattaaaga cggcgcgaaa ttggtggaat gcctggacga
    14461 catcctgaac gaatgcccgg ggctattgca aaatacgggt gcttcatcat attctataaa
    14521 taaggatacg cccgacacgg gcagtcgcgc cgttcagacg gcatacgccc cgccgcctgc
    14581 cgcaaaaatg ccgtctgaag gggcggcagg cggcacagcc gtcggcggca tactggataa
    14641 aatgggtttc gaccccatcc atcccgacgt gcttgccgga cagttggcta tgcctgccgc
    14701 agatttgtat gccgcactgt tggaattgga attggacggc agcgttgccg caatgcccgg
    14761 cggcagatac cagcgtatcc gaacttgaac gcactttata ttaaggaaca cgaatgaccg
    14821 aagtcatcgc ctacctcatc gaacatttcc aagatttcga tacctgtccg ccgcccgaag
    14881 acttgggtat gctgcttgaa gaagcgggtt tcgatacgat ggaaatcggc aacaccctga
    14941 tgatgatgga agtattgctc aacagctccg aattttccgc cgaacctgcc gacagcggcg
    15001 cattgcgcgt gtacagcaaa gaagaaaccg acaacctgcc gcaggaagtg atggggctga
    15061 tgcagtatct gattgaagaa aaagccgtca gctgcgaaca gcgggaaatc atcatccacg
    15121 cgctcatgca cattccgggc gacgaaatta ccgtagatac cgccaaagtg ctgaccctgc
    15181 tgcttttatg ggcaaacaag agcgagctgc ccgtgttggt cggcgacgag ctgatgagcg
    15241 cgcttttact cgacaacaaa cccacgatga actgaagcgg cttcagacgg cccgcccgag
    15301 tccgtctgaa acgtcggcat caaaaccacc atccagagaa cgacaaatgg cgaaaaacct
    15361 attaatcgtc gaatccccgt ccaaagccaa aaccctgaaa aaatatttgg gcggcgattt
    15421 tgaaatcctt gcatcctacg gacacgtccg cgacctcgtc cccaaaagcg gcgcggtcga
    15481 tcccgacaac ggctttgcga tgaaatacca actcatcagc cgcaacggca aacacgtcga
    15541 tgccatcgtc gccggagcca aagaagccga aaacatctac ctcgccaccg acccggatag
    15601 ggaaggcgaa gcgatttctt ggcatctttt ggaaatcctc aaatccaaac gcggcctgaa
    15661 aaacatcaag ccgcagcgtg tcgtgttcca cgaaatcacc aaaaacgccg tgctcgatgc
    15721 cgttgcccat ccgcgcgaaa tcgaaatgga cttggtcgat gcgcaacaag cccgtcgcgc
    15781 tttggactat ttggtcggct ttaacctttc gccattgtta tggaaaaaaa tccgccgcgg
    15841 tttgagcgcg ggtcgtgtgc aaagtcccgc tttgcgcctg atttgtgagc gtgaaaacga
    15901 aatccgcgcg tttgaagcgc aggaatattg gacggtgcat ctcgacagcc acaaaggccg
    15961 cagcaagttt accgccaaac tcgcccaata caacggcgcg aaactcgaac aattcgacct
    16021 gccgaacgaa gccgcgcaag ccgacgtgtt gaaagaactc gaaggcaaag aggccgtcgt
    16081 taccgccatt gaaaagaaaa agcgcagccg caatcccgcc gcgccgttca ccacttctac
    16141 catgcagcag gatgccgtgc gcaaactcgg cttcaccacc gaccgcacca tgcgtaccgc
    16201 ccaacagctt tatgaaggta tagacgtagg gcagggcgcg atcggtctga ttacctatat
    16261 gcgtaccgac agcgtgaatt tggcggatga agccttaacc gaaatccgcc attacatcga
    16321 aaacaaaatc ggcaaagaat atctgccgag tgccgccaaa caatacaaaa ccaaatccaa
    16381 aaacgcccaa gaagcgcacg aagccatccg cccgacttcc gtgtaccgca cgcccgaaag
    16441 cgtcaaaccc ttcctgagcg cagaccagtt caaactctat caaatgattt ggcagcgtac
    16501 cgtcgcctgt cagatgacgc ccgccaaatt cgaccaaacc accgtcgata ttaccgtcgg
    16561 caaaggcgta ttccgcgtaa ccggacaagt gcagactttc gcaggcttcc tcagcgttta
    16621 cgaagaaagc agcgacgatg aagaaggcga agacagcaaa aaactgcccg aaatgagcga
    16681 aggcgacaaa ttgcccgtgg acaaactcta cggcgaacaa cactttacca ctccgccgcc
    16741 acgctacaac gaagccacgc tggttaaagc cctcgaagaa tacggcatcg gccgcccctc
    16801 gacctacgcc agcatcatct ccacgctcaa agaccgcgaa tacgttaccc ttgagcaaaa
    16861 acgcttcatg cccaccgaca caggcgacat cgtcaataaa ttcctgaccg aacacttcgc
    16921 ccaatacgtc gattaccact tcactgccaa actcgaagac cagcttgacg aaattgccga
    16981 cggcaaacgc caatggattc ccttgatgga caaattctgg aaaccgttca tcaaacaagt
    17041 ggaagaaaaa gaaggcatcg aacgcgccaa atttaccacg caggaacttg acgaaacctg
    17101 ccccaaatgc ggcgaacaca aactgcaaat caagttcggc aaaatgggtc gcttcgttgc
    17161 ctgcgccggt tatccagagt gcagctacac ccgcaacgtc aacgaaactg ccgaagaagc
    17221 cgccgagcgc atcgccaaag ccgaagcaga gcaagccgaa cttgacgggc gcgaatgccc
    17281 caaatgcgga ggacggctgg tgtacaaata cagccgaacc ggcagcaaat tcatcggctg
    17341 cgccaactat ccgaaatgca aacacgtcga gccgttggaa aaaccgaaag acaccggcgt
    17401 ccaatgcccg caatgcaaaa aaggcaacct cgtcgagcgc aaatcccgct acggcaaact
    17461 gttttacagt tgcagcacct atcccgactg caactacgcc acctggaacc cgcccgttgc
    17521 agaaacctgt ccaaaatgcg cgtggccggt tttgaccatc aaaaccacca aacgctgggg
    17581 tgtggaaaaa gtctgcccgc aaaaagaatg cggctggaaa gaacagattg aaccgcctgc
    17641 accgaaagag tgaagcggtt ggggttgaga tgaaagaaaa tgccgtctga aaggggtttc
    17701 agacggcatt ttcttcatgt gaaaacaaac ctactcagat tgaaactgtt tacacacttc
    17761 tatattaatc tgtataaagt cctgccacga catttgttct gataagtaat ggtgataata
    17821 gtcctccaca cgacagacca aagggcgcgt ttcatagata gtgcataagc gcgttgaatc
    17881 atcgaaatgg cggcaggtac catctccccg atccaggtat atggtttgtt cgcttaaatt
    17941 gaccttttga cagcacaaac cgcaagcaga gcatgggaag ggttctgatg aggcaatcaa
    18001 aaatcgatta ctttcacatt gtcgtttttc aaagctgcaa tcagattgtc cacctgtttc
    18061 agtggagaat ctttggcaat gctcacaatg ccgttaattg ctgtatcaat gacgtggata
    18121 tttcccgctt ccattccttt gtccaatgcc tcaaataaac cgtcaatcat tttgcgacgt
    18181 tctttgaacg tattttccag atacaagcgc aaaatttctt gttggttttt caattctgct
    18241 aatcggacat cacgccatgc accaatgact tcacgtttgg ttttctcttg tgcagcaatt
    18301 ttcttatatt cgttgcgtgc ttctaagatg cgaaaaaacg aattagaagc tgcatcaaac
    18361 ggaatggttg ctggattctt tgttggtagc ttattaagga tttggatttt tcccattata
    18421 ctgtacctaa ttcgatattc agacaacctt cgcattgatg acggatattg gctacaggag
    18481 atccgtcctc ttccataata ggcgtattca gcagattttt aatcgccttg ccgatgacca
    18541 gcatggtatg gaaatcagga tggtcggcat ggtcgacacg aaccttttcg aaacgtttaa
    18601 ccatttcttc taaggtatgg cgtatttccc ttgcgttttc cttcaaccct tctaatcctt
    18661 ctgtaattaa atccatctcg tcaatagcct catcgatttc agcactatat gctcgtgctt
    18721 ccgtcagtgc ctcttccgcc ttgcttgcca gcataaatcc accgattgct aaagcaggtc
    18781 ctgccacgat gccacccaaa gccaccatgc cgcctgccat accgaatcca cctgctgcca
    18841 atgagccacc tccgagccaa gcaagggttg cattggttgc cgcagcacct gaaagggtgg
    18901 agattgctgt tcctgtggaa gcagttgcaa tcataggtac tgcaccgtat acgccaaagc
    18961 ctgccagtgc gccagtaatg gcactactgc cgagattggt gcttaactct aaagaagttt
    19021 gcaccatagt ttccatttct tttaattcag caatatcaaa tgtactgtta aaacccgaca
    19081 gttcgctttt cgcatgcttg ttgctggtgt tgtttaggac ttgaacaata tgtctgattt
    19141 gattgttaaa aatatcaacc ttaaactgtc ccagctcctc aaaagcaagg ttggtttcgt
    19201 ctttagtcga attcaattct cttttcctgc gtttataacg gcgttctgca cgctcaccta
    19261 tttcttttgc atcatctata tcgctgagtg cagctgctcc tttgacaata cctgttgcag
    19321 caagggcggc ggctgcaccc caaagtaaaa ctggaatagc catgtgtaat ttcctaaagt
    19381 gtataaaaag ttaaaaatct tgaagtttca atgtgtattg taattttaaa attctattaa
    19441 aaatacattt gccattccag caaactaaag tttaaaatct atccaaataa tttaaccatc
    19501 tatacagaaa gttcagctca tggaaaccca cgaaaaaatc cgcctgatgc gcgaattgaa
    19561 caaatggtcg caggaggata tggcggaaaa gctggcgatg tcggcaggcg ggtatgccaa
    19621 aatcgaacgg ggcgaaacgc agttgaatat cccgcgtttg gagcagttgg ctcagatttt
    19681 caaaatcgat atgtgggact tgctcaaatc gggcggcggc gggatggtgt ttcagattaa
    19741 tgaaggtgat agtggtggcg atattgcgtt gtatgcgtca ggtgatgttt cgatgaaaat
    19801 agaattttta aaaatggagt tgaaacattg taaagaaatg ttggaacaaa aagacaaaga
    19861 aatcgagttg ctccgcaagc tgaccgaaac cgtttaaaca gatatgccgt ctgaaaaaag
    19921 ttttcagacg gcatattctt tgacaggtct tgtataatac cgtttgaact tacaggtttt
    19981 tgattatggc ggcaggcaaa cataccaaac acagcaacca ggtacgcatt atcggcgggc
    20041 aatgtcgggg caggaaattg agttttgcat ccgccaacgg actgcgtcca acccccgata
    20101 gcgtgcgtga aaagctgttt aactggctgg gacaggattt gacgggtaaa acggttttgg
    20161 atctcttcgg aggcagcggc gcactcggta tagaagccgc ttcgcgcaac gccaaacgcg
    20221 tgctgatttc ggataacaac cgccaaaccg tgcagacctt gcagaaaaac agtcgcgaac
    20281 tgggtttggg gcaggtgcaa atcgtctgtt cagacggcat cgcatatttg aagaccgtat
    20341 ccgaacagtt tgatgtcgtc tttctcgacc cgccgtttgc atggcaggac tggcaaatcc
    20401 tgttcgatgc cttgaagccg tgcctgaacc cccgggcatt cgtctatctc gaggcgggta
    20461 ggctgccgga tatccccgat tggctgacgg aatatagaga agggaaatca gggcagagta
    20521 cattcgaatt aagggttttc caagtggctg aataatatgc gctttgataa tcatttccga
    20581 gttgtaaaca ttcgtttgca accgtccggt tcaaaaaaac cttgtgctat aatccgcgcc
    20641 cgcccggttt tgataattta gtggaaaagg aaaagaaatg tcgcttttta ttaccgacga
    20701 gtgcatcaac tgcgacgtat gcgaacccga atgccccaat gatgccattt cccaaggcga
    20761 ggaaatttac gaaatcaacc ccaacctctg cacgcagtgc gtcggacact acgatgagcc
    20821 gcagtgccag caggtttgcc cggtggactg catcctgatt gacgaagaac atcccgaaac
    20881 ccatgacgag ttgatggcga aatacgaaaa gattatccag tttaaataaa ttctttttaa
    20941 aacatcaaat tatgtctgtt ttgaaataaa atcaaaaaaa aacttgacgg aaaagcaagc
    21001 cgctaataaa ctaacgttct cttttggagg gattcccgag cggtcaaagg gggcagactg
    21061 taaatctgtt gcgaaagctt cgaaggttcg aatccttctc cctccaccaa aattcttact
    21121 tggggcagta gcgagtaatg cgggtgtagc tcaatggtag agcagaagcc ttccaagctt
    21181 acggtgaggg ttcgattccc ttcacccgct ccaaacaatt aggcccatgt agctcagggg
    21241 tagagcactc ccttggtaag ggagaggccg gcagttcaaa tctgcccatg ggcaccatct
    21301 ctcgattatt catttcttta aggcttagat atataggata ttgccatggc taaggaaaaa
    21361 ttcgaacgta gcaaaccgca cgtaaacgtt ggcaccatcg gtcacgttga ccatggtaaa
    21421 accaccctga ctgccgcttt gactactatt ttggctaaaa aattcggcgg tgctgcaaaa
    21481 gcttacgacc aaatcgacaa cgcacccgaa gaaaaagcac gcggtattac cattaacacc
    21541 tcgcacgtgg aatacgaaac cgaaacccgc cactacgcac acgtagactg cccgggccac
    21601 gccgactacg ttaaaaacat gattaccggc gccgcacaaa tggacggtgc aatcctggta
    21661 tgttccgctg ctgacggccc tatgccgcaa acccgcgaac acatcctgct ggctcg
//
LOCUS       DAXHPG010000016        21596 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXHPG010000016 DAXHPG010000000
VERSION     DAXHPG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.016"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(49..1215)
                     /gene="odhB"
                     /locus_tag="ACFM6F_000446"
     CDS             complement(49..1215)
                     /gene="odhB"
                     /locus_tag="ACFM6F_000446"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC6163922.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPVAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAGARPEE
                     RVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGVKLGFM
                     SFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDADQMSIA
                     DIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAT
                     KERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLDL"
     gene            complement(1315..4143)
                     /locus_tag="ACFM6F_000447"
     CDS             complement(1315..4143)
                     /locus_tag="ACFM6F_000447"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC6163923.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMTLQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNASKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNGSVKRVVLCAGQVYYDLEAGRTERKLEDDVAIVRVE
                     QLYPFPYEEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4348..5631)
                     /gene="gltA"
                     /locus_tag="ACFM6F_000448"
     CDS             complement(4348..5631)
                     /gene="gltA"
                     /locus_tag="ACFM6F_000448"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC6163924.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(5743..5991)
                     /locus_tag="ACFM6F_000449"
     CDS             complement(5743..5991)
                     /locus_tag="ACFM6F_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC6163925.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(5995..6702)
                     /locus_tag="ACFM6F_000450"
     CDS             complement(5995..6702)
                     /locus_tag="ACFM6F_000450"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC6163926.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDDLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6822..8585)
                     /gene="sdhA"
                     /locus_tag="ACFM6F_000451"
     CDS             complement(6822..8585)
                     /gene="sdhA"
                     /locus_tag="ACFM6F_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC6163927.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGEDYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8588..8929)
                     /gene="sdhD"
                     /locus_tag="ACFM6F_000452"
     CDS             complement(8588..8929)
                     /gene="sdhD"
                     /locus_tag="ACFM6F_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC6163928.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            complement(8923..9300)
                     /gene="sdhC"
                     /locus_tag="ACFM6F_000453"
     CDS             complement(8923..9300)
                     /gene="sdhC"
                     /locus_tag="ACFM6F_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC6163929.1"
                     /translation="MSVKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9583..10986)
                     /locus_tag="ACFM6F_000454"
     CDS             complement(9583..10986)
                     /locus_tag="ACFM6F_000454"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6163930.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDDHTQITAKSFVIATGSRPVILPQWQSLGDRL
                     IINDDVFSWDTLPKSVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVYWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIDKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTNPQIGFVGLKYAQVTAQYQPDEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11235..11972)
                     /locus_tag="ACFM6F_000455"
     CDS             complement(11235..11972)
                     /locus_tag="ACFM6F_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC6163931.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPNWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12248..14524)
                     /gene="metE"
                     /locus_tag="ACFM6F_000456"
     CDS             complement(12248..14524)
                     /gene="metE"
                     /locus_tag="ACFM6F_000456"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC6163932.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQVAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARSAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     QYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(14662..15540)
                     /gene="metF"
                     /locus_tag="ACFM6F_000457"
     CDS             complement(14662..15540)
                     /gene="metF"
                     /locus_tag="ACFM6F_000457"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC6163933.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15740..16015
                     /locus_tag="ACFM6F_000458"
     CDS             15740..16015
                     /locus_tag="ACFM6F_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC6163934.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16015..16140
                     /gene="ykgO"
                     /locus_tag="ACFM6F_000459"
     CDS             16015..16140
                     /gene="ykgO"
                     /locus_tag="ACFM6F_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC6163935.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(16246..16446)
                     /locus_tag="ACFM6F_000460"
     CDS             complement(16246..16446)
                     /locus_tag="ACFM6F_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163936.1"
                     /translation="MIGRLFRIFFFFALAALIMNRLFSRRQKRALREVAEISARVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            16589..17728
                     /locus_tag="ACFM6F_000461"
     CDS             16589..17728
                     /locus_tag="ACFM6F_000461"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC6163937.1"
                     /translation="MSQNASVGIVMPQKIPFEVPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPLVTVKDWVKSQAALADYLSIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGDSLTRALQNVKAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKGCQQ"
     gene            17725..18306
                     /gene="metW"
                     /locus_tag="ACFM6F_000462"
     CDS             17725..18306
                     /gene="metW"
                     /locus_tag="ACFM6F_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC6163938.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            18423..19268
                     /locus_tag="ACFM6F_000463"
     CDS             18423..19268
                     /locus_tag="ACFM6F_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
                     /protein_id="HFC6163939.1"
                     /translation="MKNKTSSLLLWLAAIMLTACSPNKEDKTKETGASAASSPASAAS
                     SSAPQTDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPDSGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLTQGSEETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(19317..19877)
                     /gene="efp"
                     /locus_tag="ACFM6F_000464"
     CDS             complement(19317..19877)
                     /gene="efp"
                     /locus_tag="ACFM6F_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC6163940.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(19920..21068)
                     /gene="earP"
                     /locus_tag="ACFM6F_000465"
     CDS             complement(19920..21068)
                     /gene="earP"
                     /locus_tag="ACFM6F_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC6163941.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTALVPDIVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSLQESVQKYFWFMGFSEKSGGLIRERDYRNA
                     VRFDTEALRERLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYSPETASVHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(21087..21365)
                     /locus_tag="ACFM6F_000466"
     CDS             complement(21087..21365)
                     /locus_tag="ACFM6F_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163942.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKLQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
BASE COUNT         5333 a         5492 c         5454 g         5317 t
ORIGIN      
        1 aaattcagac ggccttttca ttaacaaata aaaggccgtc tgaaacgatt acagatccaa
       61 caacaggcgg gctgggtctt ccaacgcgtc tttaatggct accaaagtca atacagcttc
      121 gcgaccgtcg atgatacggt ggtcgtaaga cagagccaga tacatcattg gacggacaac
      181 aacttgaccg ttttcaacca cagcgcgctc tttagtggca tgcataccca aaatcgcaga
      241 ttgaggcggg ttgatgatcg gggtagacat catagaaccg aaagtaccgc cgttggtaat
      301 actgaatgta ccgccggtca gatcttcgat agcgattttg ccatctttgg cttttttcgc
      361 gtaatcaaca attgcttgtt cgatgtcggc aatgctcatt tggtcggcat cgcgcaggat
      421 tggcacaacc aaaccgcgtg ggctgccaat cgcgataccg atgtcgaagt agccgtggta
      481 cacgatgtct ttgccgtcaa cagaagcatt cacaaccggg tattttttca gggcggcgac
      541 agcggcttta acgaagaagg acataaagcc caatttcacg ccgtgttctt tctcgaattt
      601 ttctttgtac ttcgcacgca agtccatgat tggtttcatg ttgacttcgt tgaatgtagt
      661 cagaatggcg ttttcttgtt gagaagccaa gaggcgttct gcaacacggg cacgcaggcg
      721 gctcattggt acgcgttctt caggacgtgc gccggcaggg gctgcggctg cggcaggttt
      781 ggcagcggca ttttgtacgt cttctttcaa tacgcgaccg tcacggccgg aaccttgcaa
      841 tgcgttcacg tcaacaccgg tctccgcagc cagtttggcg gcggcaggca tagcggcgtt
      901 gttttgtgcg gcagcaggag cggcagccgg ggcggcttcg gcaggagcgg ctacgggtgc
      961 ttcggcagcg gcagtagcag ctgtatcgat gcgtgccaaa acttggtcgg caacaacggt
     1021 ttcaccgtct tgcgctacga tttcaaccaa tacgccggct tgtggggaag ggacttccaa
     1081 aaccactttg tccgtttcga tatcgatcag gatttcgtca cgggcaactg cttcgccaac
     1141 ttttttcttc cattccaaga gcgtgccttc agatacgctt tcagacagca taggtacttt
     1201 tacatcaata atcattttgt gtctccaatg gccctttcag acggcctgtt gtgtgttttt
     1261 cattctgcat atgggcattc cgcctgagca gcctcagacg gaatgccact tggcttacaa
     1321 cgccaaagcg tcttcaacca attgtttcaa ttgggcaata tgtttgcttg agtagcccac
     1381 tgcaggcgat gcgctgcttg gacgaccggc ataagacagt ttttgctctt cgctgataac
     1441 gtcttcgatg cggtggcgga tttggtagaa cgcaccttgg tttttcggct cttcttgtgc
     1501 ccaaaccaca gattttgcgt tcggatattt cgccagttcg gctttaacct cctcgtatgg
     1561 gaacggatac agctgctcaa cgcggacaat cgcaacatca tcttccagtt tacgctcggt
     1621 acggcctgct tccaagtcat agtaaacctg accggcacac aataccacac gtttcacgct
     1681 gccgttgctt gcgcgttcgg cggtatcacc gataaccgga cggaaagtcg aaccttcggt
     1741 gaagttttcc agcgggctca ttgcaccttt gaagcgcaac aggcgtttgg acatgaaaat
     1801 caccagcggt ttgcggtatg aacccaaaac ttggcgttgc aagaggtgga acatttgcga
     1861 cgcttcagac ggcatgatga cttgcatatt gttctcggaa cacagttgca accaacgttc
     1921 tacgcgtgca gaggagtgct cggggccttg accgtcgtag ccgtgcggca ggatggtggt
     1981 cagaccgcac aaacgacccc atttggtttc gcctgaagac aggaattggt caatagtcac
     2041 ttgcgcgccg ttggcgaagt caccgaattg agcctcccaa atggtcagct tgtcaggcgc
     2101 ggagcaggca aagccgtact cgaacgccat cacggcttct tcgttcaaaa tggagtcgat
     2161 aaccaggaac tcgcccatac cttcgcccat atggcgcaga gggacataag taccgtcgtc
     2221 ccatttttcg cgtttttgat cgtgcaatac cgcatgacgg tgtgagaatg taccgcgccc
     2281 agagtcctcg ccggaaatac gcacgccatg gcctttggtt acgaggctgg cgtatgcgag
     2341 ggtttccgcc ataccccagt cgatggcttg tttgccggat gccatggctt tacgcgcttc
     2401 aatcacacgt tttgcagtcg gatgcagggc aaagccttcc ggtacggcgg taaacttctc
     2461 agtgagacgc tcaatatctg cggcaggcaa accggtttcg atgtgttcgc gccaatcttt
     2521 gccttggtat ttgctccagt cgatttgcgt gcgttgaaag ttgctcaacg ttgtttgctc
     2581 gacgtgttcg cctttgtcca aagcatcacg gtaggcttgg atgtaaccgt ctgcctcggc
     2641 ttgggttacc acgccttcgg caatcagttg ctcggtgtac aaagcacgcg caccggggtg
     2701 ttgcgatact tttttgtaca tcatcggttg ggtcaaggtc ggatcatcgc cctcgttgtg
     2761 accccattta cggtagcaga caacgtcaat cacgatgtct ttatggaatt ttttgcggta
     2821 atccaaagcg gcttggatgg caaagcaaac acgctcagga tcatcgccgt tcacatggat
     2881 aaccggagcg gaaaccattt ttgcgatatc ggtacagtgt acggttgaac gggtatcgcg
     2941 gatatcggaa gtggtaaagc cgatttggtt gttgatgacg atatgaaccg taccgccggt
     3001 ggtataaccg cgcgttttag acaggttgaa tgtcgcttgg ttgactccca gaccgataaa
     3061 tgcggagtcg ccgtgaatca ataccggcaa gactttgtcg cggccgtttt cgcccaaacg
     3121 tttttgtttg gcgcgcgcag aaccttccac caccgggttg acgatttcta agtgagacgg
     3181 gttgaacgcc aaagaaacat gcatcgggcc gtgcggggta gcaatatcgg agctgaagcc
     3241 catatggtat ttcacgtcgc cgctgggcag tttgatttcg gcacgacctt caaattcggc
     3301 aaacaaatcg ccgggttttt tgcccaaaat gttcaccaaa acattcagac ggccacggtg
     3361 cgccataccg atgatgactt cttccacgcc gtctttactg gcgttttgaa tcaggtagtt
     3421 caaaccggca atcgcgcttt cgccgccttc gacaccgaaa cgtttctgac cgacatattt
     3481 ggtatgcaga taacgttcca aagtttcggc agcagtcatc tctttcaaga tacggcgttt
     3541 ttgatcggca ttgtaatgcg gtgtggacaa tacgctttca aaataattgc gtacccagcg
     3601 gcgctcttcg gtattgggaa tatagatata ttccaatgcg atgtggccgc agtaggtttg
     3661 tttgaggttg ctgatgattt gggacaaagg cagtttgccg cgattggcaa aatcgccctc
     3721 gcccatattg aattgaagcg tcatatcggc atctgacaga ccgtggaatt tcggatcgag
     3781 ggcttcaata tcgcgcgggg ggatacgttt gagcggatca agttgggctg cacccacgcc
     3841 ttggatacga taggcagaaa tcagccgtaa aacgctgact tgctttttca gcattgcctc
     3901 atccgcaccg cccgcaacgg cagatgcaat tttctttttc gccaaagtaa caaatgattc
     3961 gcgaatcggt gtgtgtgcga catcgacagc aaccgtcccc ggctgtttgc tcaaatcggt
     4021 gaaatactgc ttccattttt catcaaccgc atcggggttt tccaaaaaag cctcgtacaa
     4081 ttcctcaatg tagggtgcgt tcgaaccgaa caggtaagag aaattgagtt tttcgtccat
     4141 catggcgtgc gctcttttct ttaaaatata aacaaagggt agaagtccgc gtcctgacgg
     4201 ctgaactgat gccgtctgaa aagacatttt attctaccct attttaaacc ggattgcctg
     4261 tattgatttc atccgactac aatcaaatgt atacagaaag caatccgatt ctctaatatt
     4321 gcctgtttga caatatatta atgtatgtta cctctctcct gccggcacat aatcgcggcg
     4381 ttccgaacca gtataaagct ggcgcgggcg gccgattttc agcgaaggat cgctgatcat
     4441 ctcgtgccag tgcgaaatcc aacctacgct gcgcgacagg gcgaagatga cggtaaacat
     4501 ttcggtcggg atgcccagcg cggacaggac gatgccggaa tagaaatcga cgtttggata
     4561 cagcttgcgt tcgataaaga acgggtcttt cagcgcaatc tgttccaatt ccatcgccag
     4621 tttgaatttc ggactgtctt ccaagcccaa ttccttcaaa acttcatagc aggtttcgcg
     4681 cataatgctg gcacgcggat ccatattgcg gtacacgcgg tgaccgaagc ccatcagacg
     4741 gtatttgcgt tgtttcacac cttccatgta tgcggcaaca ttagacacat cgccgatttc
     4801 gtccaacatt ttcaacacgg cttcgttcgc accgccgtgc gaagcacccc acaggcaggc
     4861 gataccggca gcaatacagg caaacggatt cgcacccgaa gagcctgcca gacggacggt
     4921 tgaagttgag gcgttttgct cgtggtcggc atgcaaaata aagatgcggt cgagcgcgcg
     4981 tgccaaaacg ggattgggtt tgtagtcttc acacggcgtg gcgaacatca tatgaaggaa
     5041 gttttcggaa taagaaagat tattcttcgg ataattgaac ggcagaccgt ttgaatagcg
     5101 gtagcacatt gccgcaatgg tcgggatttt ggaaatcagg cggtaaatcg cgattttgcg
     5161 gtgttcggga ttgctaatgt ccaagctgtc ttggtagaac gcagacagcg cgccgaccac
     5221 gccgaccatc atcgccatcg gatgcgcgtc gcggcggaac ccccggaaga accaagtcag
     5281 ctgttcatgc accatcgtgt ggcggcggac ggtattgtca aattctgcct tttgctcggg
     5341 agtcggcagt tcgccgtaaa tcaacaggta gcagacttcc aaataatcgg atttttcggc
     5401 cagctgctcg atggggtatc cgcgataata aagcaagcct tgatcgccgt cgatgtaagt
     5461 aattttagac tcacagcttg cggttgaaac aaatccgggg tcgaaggaaa acaaacctgt
     5521 attttttgtc agcccccgaa tgtcaaccac atcgtgcccg atgctggctt ccaataccgg
     5581 cagctccaaa cctgcctgac ccggtacgtt gagtttgatt gatttggaca tattattgct
     5641 cctttgcaag ttataaccca cgttactgca tccgcagcac gttctttgcc ttcagacggc
     5701 ttttgcattt tgaaatctgc ctgcaggcag atttcagatg cctcatgccc gtctgatttt
     5761 ttcaagcatc gggataaggt gccctttgtc cgtttccgaa tgcccgttaa tcaaggcaag
     5821 caattcttga tcttgaaatt caaggatttc ggaaaactcg gacagctctt tatcgctcaa
     5881 atgctcgaat tctttttcca taaacctgcc gaagattaaa tctaactcca acaatccccg
     5941 gcgggtttga aaacggattt tccgtttggc aatatcgtca aaaaccatca tttcttaaac
     6001 ggcccgcttc aacataatct ctttaatctt accgatggct cgggtcggat tcaagtgttt
     6061 ggggcatacg tccacacagt tcataatggt gtggcagcgg aacaaacggt atgggtcgtt
     6121 cagatcatcc aaacgctcat tagtgatggt atcacggctg tccgcaatga aacggtaagc
     6181 attcagcaaa ccggacggac cgacgaattt atcagggttc caccaaaatg acgggcaggc
     6241 agtcgaacag caggcgcaca aaatacactc gtacaaaccg tccaactctt tacgctcttc
     6301 ctgagtttgc agacgctctt tgtccgcatc aatcggatta tcgttgacaa cataaggttt
     6361 gacggaatgg tattgtttga agaactgggt catatccaca atcaggtcgc ggataacagg
     6421 cagacctggc agaggacgaa ttttaaccgg ctgtttcaag ccacgcagat cggtcagaca
     6481 cgccaaaccg tttttgccgt tgatgttcat accgtccgat ccgcaaatgc cttcgcggca
     6541 ggaacggcgg aaagacaatg tatcatcttg tgctttcagg cgcactaaag cgtccaaaag
     6601 tttaacgtcg gttggttcca attccaactc gtaacgctgc atataaggtt tggcatcaac
     6661 gtccgggttg taacggtaaa tttcaaaact cattttttcc atgagaagtg ttccttttct
     6721 cataaactgc ccaatgattt tgtattatga attcaagcag ttcaatgtgg gtatggttag
     6781 atttcagacg acctttgagg cgaagaccgt ctgaaaacgc atcaataaac gcgcttggcc
     6841 ggtttgatgt attccacgct caagggcttg gtatgcaccg gtttgtaaga caaggtattg
     6901 gtatctgaat ggtacagcgt atgtttcatc cagttttcat catcgcgctc aggatggtcg
     6961 tctgaagcgt gcgcaccgcg tgattcttta cgtgcttcgg cagacaccaa agtcgctttc
     7021 gccacttcaa tcaggttatc caattccaaa gcctcgatac gcgcggtatt ccacactttg
     7081 ctcttgtctt tgatttcggt acgtttcaca cgctcggcaa tcgccatgac ttctcgaacg
     7141 cctttgctca gaatctcatc agtacggaac acgccggcgt gcagttgtac agagcgttgc
     7201 agttcgcgac gcaatgcatc aacgttttca ccatcggttt ggttgtccaa acgctcgata
     7261 cgttggcggg tcaactcacc tgcattagca ggcaaaggtt tccagtcgct ttgctctttg
     7321 atgaatttaa tcatgctgtc gccggcagct ttaccgaata ccaccaagtc caacagggag
     7381 ttggtaccca agcggttcgc accgtgtacg gaagcacaag cgcactcacc tgccgcatac
     7441 agacctttta caggcacttc gtaatcttca ccttgcggaa cgacaacttc gccgtggtaa
     7501 ttggtcggaa tgccgcccat catatagtgg gtagtcggca caacgggaat cgggtcttta
     7561 atcggatcga taccggcgaa ctgaatggaa atctcgcgga tgcccggcag tttttccata
     7621 attttttctg cgccgatatg gtcgattttc agtaagacat ggtctttgtt tttaccgcag
     7681 ccgcgacctt cgtagatttc catcgccatc gcgcgggaaa caacgtcgcg agaagccaag
     7741 tcttttacgg tcggcgcata gcgttccata aagcgttcgc cgtcggcatt caacagaata
     7801 ccgccctcgc cgcgtacgcc ttcggtaatc aacacgcccg cacccgccac gccggtcggg
     7861 tggaattgcc agaattccat gtcttccaac gggatacctg cacgcgcaca aatacccaaa
     7921 ccatcgccgg tattcatata ggcattggta gaagacgcat aaatacgacc gccgccgccg
     7981 gtagcaaaca tcacagcttt agcgtggaaa atataaactt cgccggtttc catttccatg
     8041 gcggttacgc cgacgacatc gccgttttca tcacgaatca aatcttgtgc cgtccattcc
     8101 acaaagaatt gcgtattggc acggacgttt tgttggtaca aagtatgcag catcgcatga
     8161 cctgtacggt cggcaaccgc acaggcgcgt tctaccgcgc gtttaccgtg ttcggcagta
     8221 tggccgccga aaggacgctg ataaatttta ccgctttcca cacggtcaaa aggcataccc
     8281 atgtgttcca actcaattac ggcttcaggc gcggcgcggc acataaactc aatcgcatct
     8341 tggtcgccca accagtcgga acctttcacg gtatcgtaca tgtgccagtc ccaacggtct
     8401 tcctgcacat tacccagaga ggcggaaata ccgccctgcg ccgctacggt atgcgaacgg
     8461 gtcgggaaca ctttagacaa aacggcacaa ttcagaccgg atttggataa ttggagggct
     8521 gcgcgtaaac ctgcaccacc accgccgaca atcacggcat caaacttgcg aacaggaaaa
     8581 cccatactta cccccaaatc actttaactg aatacacgag acagccgacc agccaaacga
     8641 tggtggcaac ctgcaaaaac aaacgcacgc cgaagggttt gatatagtcc atccacaaat
     8701 cgcggatacc cacccaagcg tgcaagaata cggcgatgaa gctcacttgg gtaaatactt
     8761 ttacccaagt ttgactaaaa aatgcctgcc atgccgaata ttctttaggc agggaaaata
     8821 gaaccactaa aagtgcaacg gtataaatca acataataac cgcagtcgca cgttgcatca
     8881 cccaatcgcg caaaccgtaa tgggcaccgg tcaatttacg ttctaccata acaacgctcc
     8941 caaaacgaca gtcaaaacca atgcagaagc aaatacggct ttagcggtat tgcgcgcagt
     9001 attcagctca aggcctttgt gcgcatccaa aaataaaaag cggataccgg caagggaatg
     9061 gtgcagataa gcccacaata cgccgattaa aaccagcttg accaaaggat gggaaacaat
     9121 ggcacggtaa gtttcaaatg cagactcttg actcagggtg ccggacagga aatacagcag
     9181 gaaaggcagc ataataaaca gcccgacccc gctgatgcgg tgaaggatgg aaactatccc
     9241 gggtatcggc agacggatgt tcggcaaatc cagataaaca ggacgcggtt tgacagacat
     9301 agtcaattcc tctgtagtta tatatcgact caaaaaccgt aaacccattg ctacactggg
     9361 ttacataaac gttaatttac ctgttttatc ccattttgaa cagtgtattt taaaaacaaa
     9421 ctgatagatt ttgacaaaca cgcgtgaaaa cagcagatta aaagaaagat ttttagtttt
     9481 gcccccttcc cgacacggta tcagcatccg acctccaacc ccatcctcaa aaataaaatg
     9541 ccgtctgaaa aaatttcaga cggcattgtt ttgccatatc ggtcaggctt tcaatttcgc
     9601 atcggcatca cgcaacgcag tacgcagacc ttcctcgata acaggatggt agaacggcat
     9661 atccagcatt tgcggaacgg tcatcttcat ttgatgcgcc catgccaaca ggtgcgccaa
     9721 atgttcggcg gcagggccta cgatttccgc accaatgaag cggccggttg ctttttcggc
     9781 atacaggcgc atatggcctt tgtttaccag catcacgcgg ctgcggcctt ggtttttaaa
     9841 cgatacttcg ccgatgacaa attcgtcggg ttggtattgc gcggtaacct gcgcgtattt
     9901 caaaccgaca aagccaattt gcggattggt aaacaccacg ccgatggtgc tgcggcgcaa
     9961 accgccgccg atattcggat agcggcccgc gttgtcgccg gcaatcttgc cttgatcggc
    10021 agcttcgtgc agcaggggca gttgattgga tgcgtcgccc gcaataaaga tatgcggaat
    10081 actggtctgc atggtcagcg gatcggcaac gggtacgccg cgcgcatctt tgtcgatatt
    10141 gatgttttcc aaaccgatat tgtcaacgtt cggacggcgg cctacggctg ccagcatata
    10201 ttcggcaaca aatacgcctt tttcgccgtc ttgctcccaa tagacttcta cattgccgtc
    10261 cgcatcgagt ttgacctcgg ttttggcatc cagatgcagt ttcaattctt caccgaacac
    10321 ggctttcgcc tcgtctgaaa cgacagggtc ggaaatgccg ccgatgatgc cgcccaagcc
    10381 gaaaatctcg actttcacgc ccaaacggtg caatgcctgc cccagttcca aaccgataac
    10441 gcccggtccg aacacggcaa cgctcttagg cagcgtatcc catgagaaaa cgtcgtcatt
    10501 gataatcaaa cggtcgccca aagactgcca ctgcggcagg ataacgggac gcgagccggt
    10561 agcaatcacg aaactttttg ccgtgatttg ggtatggtcg tcgatttgga cggtatgctc
    10621 gtcgataaat ttagccgaac ccatgatgcg tttgtcagca ggccattctt ccacatcggc
    10681 aacgacaaag ccgacaaaac gatcgcgctc ggatttaacg cgctgcatca cttcttcgcc
    10741 attgacgacg atactgtctt tatccaaatg cacgccgaac gggtcggtat gcaatgcgtg
    10801 atggcgcgcc tcggcggcgg caatcaagag tttggaaggc atacagccca cgcgcgcgca
    10861 ggtcgtgccg aacacattgt tttcaatcag gtaaacattg tccgaatgta aacgggcatt
    10921 gcgaaacgcg cccataccgg cagtaccgcc gccgattacg acgacatctg cttgaatttt
    10981 tttcataatc tttgtccttc cgttttcgtc tgaaacactg ccgacacgga atatatcggc
    11041 ttcagacggc atttaaatat tttcccaatc aaaaacatta ttcagataaa taaaaacaaa
    11101 atttcgattg agtgcaacaa tatccaaatc ctcttataaa tatagagtta ttcggagcgg
    11161 tagtgtagaa ccgcccgaat aactctatcc ggtatatcag acagtttaat caaaccgcct
    11221 taagcaaaca gctatcagtt tttagccaag taagcttcca aatcttcgct gccgccgatg
    11281 tatttaccgc cgatgaagac ttgcggggca gtcatcttgc cggtaatggc gcgaacggaa
    11341 gtaacggttg catctttgcc caatacgatt tcttcgtaag acaaaccttt gtcttgcagg
    11401 gcttgtttgg ctttagcaca gaattggcag ccgggtttgg taaagatggc aacagactct
    11461 tgagccttcc aattgggagc aacgaatttc agcatagtat ctgcatcgga tactttgaac
    11521 ggatctcctg gttcttccgg ctcgatgaac attttttcaa ccacgccgtc gttaaccagc
    11581 atggagtaac gccaagaacg tttgccgaag cccaagtctt ctttaccgac caacatgccc
    11641 atgccttcgg tgaattcgcc gttgccgtca ggaatcatgt agatgttgtc ggattcttct
    11701 tcggccgccc aagcgttcat tacgaaagta tcgtttacag atacgcagta gattgcgtca
    11761 acgccgtttt ctttgaacgc gccgaacaat tcgttgtaac gcggcaggtg tgaagaagaa
    11821 caagtcgggg taaatgcacc aggcagggag aatacgacta cttttttgcc tttgaacaaa
    11881 tcatcggtag acacatcttt ccaagtgtcg ccaacgcggg tgcggaatac tacggaaggt
    11941 actttttgac cggtacgatc ttgcaaagcc attttgagct ccttaataga taggtttaag
    12001 aaatgattgt gaatcgctgg gcattctacc ccgaataagt cgatttgtat aatttatagt
    12061 tcaaataata ttaattattc aaaactatta atcaccaacc tcgaccaact tttacagtat
    12121 ataaagccat gcctaaaaaa aacaagtctt atttgagaaa aattcttaga aactccccta
    12181 cgctcatttg aaaacagaaa tgccgtctga aaagtattca gacggcatcc gatcacgtta
    12241 tccctgctta tttagccagt tcggcacgca gtttgtgggt tacgttcatc atcacttgga
    12301 gttgttccag agtttctttc caaccgcgtg ttttcaggcc gcagtctggg ttaacccaca
    12361 gacgttcaac cggtacaacc tcgatggctt tgcgcaacag gtgctccact tcggcttctg
    12421 tcggtacgcg cgggctgtgg atgtcgtaaa cccccgggcc gatgtcgttc gggtattgga
    12481 attcgccgaa cgcggtcaag agttccatgt cggaacgtga agtctcgatg gtgatcacgt
    12541 ccgcatccat tgcagcaatc gcaggcagga tatcgttgaa ctcggagtaa cacatatgag
    12601 tgtggatttg ggtgctgtct tcgcaaccgg cagaggacag gcggaaggat tcgcccgccc
    12661 agttcaggta ggcatcccaa tcggcgcgtt tcagcggcaa gccttcgcgg atggcaggtt
    12721 cgtcaatttg gatgactttg atgccggctt tttccagatc caatacttcg tcgttcagag
    12781 ccagtgcgat ttgtttgcac acggtagagc gaggaatgtc gttgcggacg aaagaccatt
    12841 gcagaatggt tacagggccg gtcaacatac ctttcatcgg gcgtttggtc aggctttgtg
    12901 cgtaagtaga ccaagccacg gtcatggctt caggacggct tacgtcgcca aagataatcg
    12961 gtggtttcac gcagcgtgag ccgtagcttt gtacccagcc gtattgagtg aatgcaaaac
    13021 cgctcaacaa ttcgccgaag tattcaacca tgtcgttacg ctcggcttcg ccgtgtacca
    13081 gtacgtccaa gtccagtttt tcttgctctt caaccaccaa ggcgatttct tttttcatcg
    13141 cggcttcgta atcggcggca gacagttcgc cttttttgaa ggctgagcgt gcctggcgga
    13201 tttcggtggt ttgcgggaaa gaaccgatgt tggtagtcgg tagcagaggt aggttcaacc
    13261 atgcttgttg cgctttgata cggtcggcaa atggagattt gcgttggtct gcgttggcag
    13321 gcaaatcggc caggcgtttg gcaacgtctg cacgatggat ttcgctgctg ttggcacggg
    13381 agtcggcagc agcttggctg gcggcgagtt cttcggcaac agaatcacgg ccttcgttca
    13441 atgcagcttt cagaacgcgc aattcttggg ttttttgcag ggtgaatgcc aaccaagagt
    13501 acaggtcggg tttgttggct ttcagttttt cttcaactga caagtcaaat ggagtgtgca
    13561 gcagcgagca agagctggaa atccacaaac ggtcacccag tttggcttgc agaggctcga
    13621 cagtttccaa aactttgttc aggttggcgc gccaaatgtt gcggccgtca atcacgccgg
    13681 cagacaggac tttgtcgtag tcggcgaacg cgtccagttg ctcgggggcg cgtaccaagt
    13741 cgatgtgcag accgtcaaca ggcagggctt tcaacaatgc ggcgtgttcg gcaacagaac
    13801 cgaagtaagt gctcaacagg attttggcac ttactttgct caaagtagcg taaacgtctt
    13861 tgtaggcttc cacccattct ttaggcaaat cgacagccaa agcaggctcg tcgatttgaa
    13921 tccactcggc accggcttca accaaagcag tcaggatttc aacgtaaaca ggcaacagtt
    13981 taggcaacag gctcagacgg tcgaattcga cggcgccttt ttctttaccc acccacagga
    14041 aagtcaacgg acctacaacg gtcggtttgg ctttcagacc gagggcttgg gcttcttgca
    14101 gttgttgaac atagtgtttg gcattggctt tgaattcggt atcggcgtgg aattcaggca
    14161 ccaagtagtg gtagttggtg tcgaaccatt tggtcatttc gatagcgaat tggtctttgt
    14221 taccgcgcgc cagttggaag aattgttcca aagacaggtt ttggctgtcg aagccgaagc
    14281 gggcgggaat cgcgccggtg gcgacttgca ggtcgaggat gtggtcgtag aaagtgaaat
    14341 cgcctacggc aacgaaatcg gcgttggcag cgacctggtg tttccagttt ttctcgcgca
    14401 agtctttagc aacagccagc aattcttgct cgctgatttc tttgcgccag tatttttctt
    14461 gtgcgaattt caattcgcgg aaggcgccga cacgcgggaa gcctgagaaa tgtaatgttg
    14521 tcatgttgaa ctctccagtt ggattttgta aggaccggtt caatccgaat cggccggtat
    14581 tgtaaatcag aatggctcgg atggggcggt gttttgcttt ttagacacct tagatgccgt
    14641 ctgaaactgt ttgatacggc tttaagggcg cacgcctaaa atatggcaaa tggcgtaagt
    14701 cagctcgctg cggttaagcg tatagaagtg gaaatctttc acgccttcgc gggacaggac
    14761 tttgaccata tcgatggcga tacttgccgc caccagattg cgcgtacctt ggtcgtcgtc
    14821 caaaccttca tacatttgcg acagccagct tgggattttg acgttggtta cttgagccat
    14881 tttaccgagc tgcttgaagt tggtaacagg caaaataccg gggacgattt ccacatcgat
    14941 acccaacatc acgcagcggt cgcggaagcg caggtagcgt tccacatcga agaagaattg
    15001 ggtgatgacg tggttcgcgc ccgcatcgat tttgcgtttc aaattaatca ggtcggcttg
    15061 tgcggatttc gcttcgggat gcacttcggg atatgctgct acagagatgt cgaagtcggc
    15121 gacggagcgt aatagcttaa ccaagtcttc ggcgtaaaac ggttttttct cataaccggc
    15181 cggctcgtct ccgcgcaggg cgacaatgcg gcggatgccg ctgtcccaat aatctttggc
    15241 aatttggcgc aattcgtcgg gagaagcgtc gataccggtc aggtgaggcg cggcttccaa
    15301 gccggtttcc tgtttgatgc gtttgacgat gccgtgtgtg cggtcgcgct cgcctgagtt
    15361 tgcaccgtaa gttacggaaa caaatttcgg gtgcaaggtt tgcaggcgat ggatggaatc
    15421 ccacagcatg gtttccattt gttcgttttt cggcgggaaa aattcgaatg aaacgttgat
    15481 gtcgcctttc aaatcggaaa ggctgttatt taacgcattg atttcttttg cgtaattcat
    15541 ggctgcgtac cttttattat cttttatatg atgtttcagg caacataata aagttgcgcc
    15601 aaacatgagt caatttcaaa attttcagat ttatcatgaa aatttctaat atattacaaa
    15661 aatagttaaa tttccttgta cagcccttct aaactatgct agagtttgtt atgttataac
    15721 actgaaagca aggcaatata tgaaaccgaa tattcatccc gacaattacc gaaccgtcct
    15781 ttttttcgat agcagcgcca atgaaggctg gttaatccgc tcctgtgccg aaacacacgg
    15841 aaaaacgatg gtttggacag acggcaaaga atatccgctg ttttcgctgg atacttcttc
    15901 cgcatcccat cccgtctata ctggcaaaca gcgcaacgtc aacaccgaag gccgtgccag
    15961 caaattcaac caacgtttcc aatccgtaat gtcatcgttt agaaaggaca aataatgcag
    16021 gttctgtctt cactcaaaac cgccaaacaa cgccaccgcg actgccaaat cgtcaggcgc
    16081 aggggcaaag tttacgtcat ctgcaaaagc aatccgcgtt ttaaagcgcg ccaacgctga
    16141 ccccctgccc ttcctgcttc ggttgtaaat cccctcctac cgcaagccct tttcaaaaaa
    16201 tgccgtctga aacagaaaaa tgcgtttcag acggcatttt ttccgtcaat acgaatccgg
    16261 aatgtgtttg aaatacagca taaacagata ccaaaacagc gtcgcggcgg ccgcaccgag
    16321 cagcacccgg gcactgattt cggcaacttc gcgcagggcg cgtttctgcc tgcggctgaa
    16381 gaggcggttc ataatcagcg cggcaagtgc gaaaaagaaa aaaatacgga aaagtctgcc
    16441 tatcatgtcg ggaagcgaaa agtatggttc gggcgcggcg gcatggggaa ggatgtttca
    16501 gcgcgtcagg ctgcttgtcc gaccgtgtca aaaagtcatg ccgtttgcga tataataacg
    16561 ctttttgccg tatttgttgg aacaggatat gagtcaaaat gcctcggtgg gcattgtaat
    16621 gccccaaaaa attccgtttg aggtgccgct ggttttggaa aacggtaaaa ctttgccgcg
    16681 tttcgatctg atgattgaaa cctacggcga gctgaatgct gaaaaaaaca atgcggtttt
    16741 aatctgccac gcgctgtcgg gcaaccatca tgttgcgggc aggcattcgg cggaagataa
    16801 atatacgggc tggtgggaca atatggtcgg acctggcaaa ccgattgata cggaacgttt
    16861 tttcgtggtc ggtttgaata atctgggcgg ctgcgacggc agcagcgggc ctttgtcgat
    16921 caatcctgaa acgggtaggg aatacggcgc ggattttccg ctggttacgg tgaaggactg
    16981 ggtaaaatcc caagccgcgc ttgccgatta tctcagcatc gggcaatggg cggcggttgt
    17041 cggcggcagc ttgggcggca tgcaggcttt gcagtgggcg atttcctatc ccgaacgcgt
    17101 gcgccacgcc ttggtgattg cgtccgcgcc gaaactgtcc acgcaaaata tcgcgttcaa
    17161 cgatgtggcg cgtcaggcga ttttgaccga tcccgatttc aacgaaggac attaccgcag
    17221 ccgcaacacc gttcccgctc ggggcttgcg gattgcccgc atgatggggc acatcaccta
    17281 tcttgccgaa gacggtttgg gcaaaaaatt cggacgcgat ttgcgttcca acggctatca
    17341 atacggctat ggcgttgaat ttgaagtaga atcctatctg cgctatcaag gcgacaaatt
    17401 cgtcgggcgg tttgatgcca acacctattt gctgatgacc aaggctttgg actatttcga
    17461 tccggcggca gatttcggcg acagcctgac ccgcgccctg caaaatgtga aagcgaaatt
    17521 ctttgtcgcc agcttcagca ccgactggcg tttcgcaccc gaacgttcgc acgaactggt
    17581 caaggcactg attgccgccc aaaaatccgt gcagtatatc gaagttaaat ccgcacacgg
    17641 gcacgatgcc tttttaatgg aagacgaagc ctatatgcgt gcggtcgccg cctatatgaa
    17701 caacgtttat aagggatgtc agcaatgaac ctgcgcgatg atttgcaact gatttacgac
    17761 cggatacccg aaggcagccg cgtcttggac ttgggctgcg gcgacggcga actgcttgcc
    17821 gcattggtcg aacacaaaaa atgcagcggc tacggcatcg aaatcgacac cgacagcgtg
    17881 attgccgcga tgtcgcgcgg cgtgaatgtt atccaagccg atttggaaga aggtttaacc
    17941 gcattcaacg atcaaagttt tgatgtgatt gtgttgagcc aaaccatcca agcgatgcag
    18001 aataccgaaa aaatcctgcg ctgcctgatg cgcgttgcca aacaggcgat tgtcagcttc
    18061 ccgaatttcg gctactggcg caaccgtgtc caaatcgcac tcggcggaca tatgccggtt
    18121 tccgaacgta tgccctacca ttggtacgac acgcccaata tccattggtg cacgctcaaa
    18181 gactttgatt tgttgtgcgc caaaaacaac atccgcgtcc tcgaacgcgc cgtgatgacg
    18241 ggcaacagac aggtcaaaca tttccccaac cttttgggca gcctggcgtt ttaccgcgtc
    18301 gggtaagaca ggttgagaac cttgtcggca ataacaggtt cttcggttct attaacaaca
    18361 ggccgtctga aaaatgaaac ggcaggtttt cagacggcct ttttaatatt ggaaacaata
    18421 gaatgaaaaa caaaacctca tcacttctct tatggcttgc cgcaatcatg ctgaccgcgt
    18481 gttccccgaa caaagaagat aaaaccaaag aaaccggcgc atctgccgct tcgtccccgg
    18541 catccgccgc ttcgtcttcc gcgccccaaa ccgatttgca accggccgca tccgcccctg
    18601 ataacgtcaa gcaggcagaa agcgcgccgc cgtcaaattg caccgacctg caccccgcca
    18661 ccggcattga cgatctcatg cagcaaatcg ccgaacacat cgactcggac tgtctgtttg
    18721 ccctttccca tcacgaactg gaaacccgtt tcggcttacc cgacagtggc tatgacaaca
    18781 tacagcggct gctgtttccc gacatccgcc ctgaagatcc cgactaccat cagaaaatca
    18841 tactggcaat tgaagacttg cgttacggaa agcgcacgat cagccggcag gcacaagatg
    18901 ccttgatgga acaggaacgc cgcctccgag aagcgacgct gttgctgaca cagggcagcg
    18961 aagaaacccg cgggcaaggc gaggagccga aacgcacgcg ttattttgaa gtttcggcaa
    19021 cccctgccta ttcgagccgg cacaacaacg gacttggcgg caatttccaa tacatcggcc
    19081 aattgcccgg ctatctgaaa atacacggag aaatgcttga aaaccaatca ctcttccggc
    19141 tgtccaaccg tgaacgcaat cccgacaaac cgtttttaga catccatttt gacgaaaatg
    19201 gcaaaatcac gcgtattgtc gtttacgaaa aaaacatcta cttcaatcca aacttggggc
    19261 gaagataaaa aaatgccgtc tgaaagcctt tcagacggca tttttcaagt aggatatcag
    19321 gcgcgtttgc ggaattcgcc ggtgcgggta tcgatttcga ctttgtcgcc gttttcgatg
    19381 taagacatca cttggatttc ggtgccgccg accagacgcg cggttttcat cactttgccg
    19441 gaagtgtcgc ctttgacagc aggctcggtg tactcgactt cgcgtacgat gatggtaggc
    19501 agttcgacag aaatggggtt gccttcgtag aaggtaactt cgcattggtc ttccataccg
    19561 tcaacgatga atttcaacgc gtcgccgatg ttgtcggctt cgatttcgta ttggttgaat
    19621 tcttcgtcca taaagacgta catcgggtcg gcaaaatagc tgtacgtaca gtttttgcgg
    19681 gacaggatga ccacgtcgaa tttgtcgtcg gctttgtaaa tggtttcgga agccgcgccg
    19741 gtcagcaggt ttttcagttt catgctgact ttggcggaag agcggccgcc tttgatgtat
    19801 tcggtttttt gaacgaccat aggatcgttg ccgaccataa atacattgcc ggcgcgcagt
    19861 tcttgtgctg ttttcattgt gattttccaa tcaaatcggt tgtcgtaaaa cgcgctattc
    19921 tagcgtattt tttgatgctt tgaaacaaag gcggcgagtt tttcggatgc ggaaggctgc
    19981 ccgaaaagat aacggctcca atcctccgcg ccttgccgcc agccgttttg atgttgttgc
    20041 agggtttgcc aacattcgag gcgttgcgtg gcggataaag cctctccgcc gttgaggtcg
    20101 tctgaaaggc ggcggtgtac cgatgcggtt tcgggcgagt aaaatccatg cgccttatcc
    20161 caaaaggcgt ggagtttatc gagatggaca ttctcgtctt gcgggtagat gtgccaaaag
    20221 aagggtttgc cggcaagctg ggcgcgcacg aaactgtctt cgccgcgtat cacggcgcag
    20281 tcggcgaggt gcagcagttt gtcgaagtcc tgttgcggca cgaaagggat tttgacgagg
    20341 cggacggatg ccgtctgaaa aacatcgccg tcgttttgca gggcatcttg cggaataacg
    20401 ccgctttgtt tgaggctgtc gatgatttgc gtccccgcca gcaacagtgt catcgggctg
    20461 cctgcctgtc gccacatttc cagccacttt gcccaaacat cgctccgata gccgaaaagc
    20521 agccattcgg gggcgttttt ttcgggaagc atcagccgct ctcgcaaggc ttcggtatcg
    20581 aaacggacgg catttcggta atcgcgttcg cgtatcaacc cgccgctttt ttcgctgaaa
    20641 cccataaacc aaaaatattt ttgaacactc tcctgcagcg aaggcatcag atgcagcctt
    20701 tcattgcttt cttccgcgct caaatattcc caattcagcc aaagcggctt gtgtcggcgg
    20761 atgatgtgca gcacattttc gggcaggtcg caggcaaaag tttcgataac gatatcggga
    20821 acaagggcag tatcgatatc cgccgcatcg gaatgccaag tgcggacatg aatatcctga
    20881 tgaacgcagg gaacatcggg caaatcaggg caaagcgcac gcaaggcgga cacatcgtcc
    20941 gtccacaaat gcacctgcca accgagttcg cggtgcaaaa cacgggcgag ccgccacgaa
    21001 acgccgatgt cgccgaaatt gtcgatgacc ttgcaaaaaa tccaacagac aaaaggagga
    21061 gtattcatag aaataacgga aacaagctaa aacgcgccga aatagtggaa caaatacaca
    21121 atcagcaaaa taatcggaat aaacagcaac atagtttgac gtttcaaatc aaactcttgt
    21181 ttcaaatgat gcttgatggt gtcgaaaagg acgaatttaa acaaaaacgt cgcttcgcag
    21241 gcaaggatga tgccgttgat cagatgctgg agtttgtctt cgggcggatt ggcggcgaaa
    21301 acggcaagcg tcgccaacgg gatgaggcag acggcaagcc aacggacaag caggtttcga
    21361 gacattgcaa ggctttcaaa aaaagcaaaa cgccattatc gccccaagcg cacgccttgt
    21421 ctaacggacg caccggagat gccgtctgaa gccttgccgc aggaaaacac cgcaccgccg
    21481 aacctttaaa tacgcccgag acgggcgttc cccgaaaaat cggacgctgc cgccgatgcc
    21541 tgaggacaag gcggcagcag gcacgggcat attgaaaacc cggcgcgtcg gtttga
//
LOCUS       DAXHPG010000017        20696 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXHPG010000017 DAXHPG010000000
VERSION     DAXHPG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.017"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(63..239)
                     /locus_tag="ACFM6F_000467"
     CDS             complement(63..239)
                     /locus_tag="ACFM6F_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163943.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVSAR"
     gene            complement(242..1600)
                     /gene="murF"
                     /locus_tag="ACFM6F_000468"
     CDS             complement(242..1600)
                     /gene="murF"
                     /locus_tag="ACFM6F_000468"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC6163944.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLAALQTLAKVWRENVNPF
                     VFGITGSVGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1725..2969)
                     /locus_tag="ACFM6F_000469"
     CDS             complement(1725..2969)
                     /locus_tag="ACFM6F_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6163945.1"
                     /translation="MEILCIGGVFADVKNYKRRSKIWLTILLTLILSCAVMDKIASDK
                     DLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKKASTWHIRHVAPKYKNYVV
                     VIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAHATNLSLPQTLGLPGEPNN
                     NIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPWFTQRGDYGKSAGLSDRLL
                     LPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARRFQYQTEKISCYVSTIAQT
                     DKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLTHGAWKRQSYGVPLVKISS
                     DDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFWGNVPDVPGEGNNLAFIDG
                     LPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(2930..3370)
                     /locus_tag="ACFM6F_000470"
     CDS             complement(2930..3370)
                     /locus_tag="ACFM6F_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163946.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVSGGYLLT"
     gene            complement(3393..4871)
                     /locus_tag="ACFM6F_000471"
     CDS             complement(3393..4871)
                     /locus_tag="ACFM6F_000471"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC6163947.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFQTPWGEGKCHTRLLGRFNAQNLAACVALLCANGYPLDKVLDVLEKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4896..6641)
                     /locus_tag="ACFM6F_000472"
     CDS             complement(4896..6641)
                     /locus_tag="ACFM6F_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC6163948.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6702..6965)
                     /gene="ftsL"
                     /locus_tag="ACFM6F_000473"
     CDS             complement(6702..6965)
                     /gene="ftsL"
                     /locus_tag="ACFM6F_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC6163949.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6962..7948)
                     /gene="rsmH"
                     /locus_tag="ACFM6F_000474"
     CDS             complement(6962..7948)
                     /gene="rsmH"
                     /locus_tag="ACFM6F_000474"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC6163950.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIAGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7945..8400)
                     /gene="mraZ"
                     /locus_tag="ACFM6F_000475"
     CDS             complement(7945..8400)
                     /gene="mraZ"
                     /locus_tag="ACFM6F_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC6163951.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            8706..9896
                     /locus_tag="ACFM6F_000476"
     CDS             8706..9896
                     /locus_tag="ACFM6F_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6163952.1"
                     /translation="MSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLIAEKIGKHGN
                     WIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLARQLQELLSQT
                     AGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQARAPEASQKVV
                     HLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTYSARPLHDLQ
                     LSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQYYHPQRN
                     QGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYLPQSHFLLNL
                     GITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVDWAGFRFGDI
                     CHKL"
     gene            10028..10104
                     /locus_tag="ACFM6F_000477"
     tRNA            10028..10104
                     /locus_tag="ACFM6F_000477"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10062..10064,aa:Met,seq:cat)
     gene            10112..10187
                     /locus_tag="ACFM6F_000478"
     tRNA            10112..10187
                     /locus_tag="ACFM6F_000478"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10145..10147,aa:Ala,seq:ggc)
     gene            complement(10469..11290)
                     /locus_tag="ACFM6F_000479"
     CDS             complement(10469..11290)
                     /locus_tag="ACFM6F_000479"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC6163953.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11323..11967)
                     /locus_tag="ACFM6F_000480"
     CDS             complement(11323..11967)
                     /locus_tag="ACFM6F_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6163954.1"
                     /translation="MKLKHLLPLLLSAALSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYRIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11970..12833)
                     /gene="ftsN"
                     /locus_tag="ACFM6F_000481"
     CDS             complement(11970..12833)
                     /gene="ftsN"
                     /locus_tag="ACFM6F_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6163955.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKQPVADKA
                     DEVEEKADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(12939..14435)
                     /locus_tag="ACFM6F_000482"
     CDS             complement(12939..14435)
                     /locus_tag="ACFM6F_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC6163956.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSHRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14449..14769)
                     /locus_tag="ACFM6F_000483"
     CDS             complement(14449..14769)
                     /locus_tag="ACFM6F_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC6163957.1"
                     /translation="MFGKQLFEEVGSKISETIVNSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15253..16779
                     /gene="putP"
                     /locus_tag="ACFM6F_000484"
     CDS             15253..16779
                     /gene="putP"
                     /locus_tag="ACFM6F_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC6163958.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWI
                     KKPAQAAGESGLLTMYEIVPGFIVCLIIAVLVSLFNKEPSREIQKRFEKADADYRAAR
                     "
     gene            complement(16869..17036)
                     /locus_tag="ACFM6F_000485"
     CDS             complement(16869..17036)
                     /locus_tag="ACFM6F_000485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163959.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            17028..20633
                     /gene="putA"
                     /locus_tag="ACFM6F_000486"
     CDS             17028..20633
                     /gene="putA"
                     /locus_tag="ACFM6F_000486"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC6163960.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDRLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSKKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDNVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4848 a         5963 c         5064 g         4821 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagagacttt cccaatcacg caaatcgggt aactccccgg atttttgccg
      121 ccacccaagt ctaaacataa atcttcataa agatacgcct gcgtctgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcaacaat aaggctttaa aaaaacgtcg acttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gagcatacgc gccgacttcg gcgtgcatgg
      481 cggcggcttc gtcctcaccc agttcgccca tatcgcccat cacgaaaata cgcggcgcag
      541 gcatacgcgc caacacgtca atcgcggctt tcatgctgtc agggttcgca ttataagtat
      601 cgtcaatcag ggttgcgccc ttgattccgg atttgacgtt cagacggcct ttgatattgc
      661 tgaagccttt caaaccttcc gccacatcgt tcaaactcaa acctgcagcc aaagacagcg
      721 cggcggcagc ggcggcgttg tggacattgt ggcggccggg aacaggcagc accacggcgg
      781 cgcgttcgtc gccgcacacc aaatcaaatt cgcacgacaa cggtttcagc acgatatttt
      841 ccgcgtggac atcgccgcta tcgatgccga aagtgcgcgt attcaaatta agcgttgccg
      901 ttttgaagac agccatattg gcatcttctt gaggaatcag tgccatgccg tctgaacata
      961 agccttgata aatctcgctt ttcgctttgg caatatcgcc cactccgtcg aaaccgcagc
     1021 cgacatgggc gcgcatggcg ttgttgacca atgcggcatc gggtttggcg atttgtgtca
     1081 aaaccgccag ttcgccaaaa tggttcatac ccatttcaat cacggcatag cggtgttttt
     1141 cgtttaattt caacaaagtc aacggcaatc cgatgtggtt gttgaagttg cctgccgtcg
     1201 ccaaaacggc attatcgccg aaacggcggc gcaataccgc agccagcatc tccttcaccg
     1261 tcgtcttgcc gaccgaaccg gtaatgccga acacaaacgg attcacattt tcacgccaca
     1321 cctttgccaa cgtttgcaat gcggcaagcg tgtcatcgac tttcaacgcg ccatccattg
     1381 caacgcaatc ttcgcgcgaa accacaaccg ccgccgcacc agcagccaat acgtcttcaa
     1441 caaaatcatg cgcatcaaac cgcccgcccg ccaatgcgaa aaacacatcg cccgcgcgga
     1501 tgtcgcggct gtcggttacg atgcgcgaca cgggtttgct ttcagacggc atcggaagct
     1561 tgagggcttg gcagatgaaa tttaggtcca gtggtttcat atttactttc gttaatattc
     1621 gggcggcgga cacatcggta gcggctgatt tttttatcgc ctgttttgct gtggtaaaac
     1681 acagattatt ttcccattct cattcggcat tttttctgta cgtatcattt tttagacgta
     1741 tttttagtcg atttgccttt tcccgcatac cacggcgcgg ggtcgtcggg cagtccgtcg
     1801 ataaaggcaa ggttattgcc ttcgcccggc acatcgggaa cattccccca aaaatcatag
     1861 ccgtcatcgg gcaactcgtc ggtttcgata cccgtccaac tgccgaatcc gcgtaaaaaa
     1921 ttaaacgcgc tgcgcctcac tttaatcatt tcgcgccgcg tgtcatcgga cgaaatttta
     1981 accagcggca cgccgtagct ttgacgcttc cacgcaccat gtgtcaacgt tcgctcgccg
     2041 cctttaccga catgcatcaa accgtggtcg gaaaagtaaa ccaaagacca gctttcttta
     2101 ttttcattca atatcttaac tgtatcttct aaaaatttat cggtttgcgc gatggtggaa
     2161 acatagcagg atattttttc agtttgatac tgaaaccgcc gcgcatcctt atccaaacgt
     2221 gtgcaaaaat cactgtgcga acccatcagg tgcatcacaa tcagccgagg cttcgtgcct
     2281 gcatttccta tcaaaacccg tttgaacgcc ggcaacaaaa ggcggtcgct caaccccgcg
     2341 cttttgccat aatcacccct ttgggtaaac cacggataat cgctgcgtag ggcataggtg
     2401 gaaatttcgt tggcaaaatg ccccaacatt ccttgattag acagccacgc cgtccgaaaa
     2461 cccgcctgct tcgccaagct gacgatgtta ttgttcggtt ctcccggcag ccccaaagtc
     2521 tgcggcagcg aaagattcgt cgcgtgggcg gtcgattggt aaccgtttat caacagccct
     2581 ttggtctgac tcaaaaaagg cgtatcgggc aatgggaaac cgtaaacatt catataatcc
     2641 gaacgcgcgc tctcaccgat aaccacaaca taatttttat acttgggcgc aacatgacgg
     2701 atatgccatg ttgacgcttt ttttgctgct tccaaaatgt gggcgcgctt ggcggcatat
     2761 tgtgccggcg cggaagccaa atcgtaatac aggtcgaaaa tattcaacaa caggccggca
     2821 tcaggttctc gcaaatcttt atcgctggcg attttatcca tcaccgcgca ggacaaaatc
     2881 aaagtcaata atatagtcag ccatattttg ctgcggcgtt tatagttttt tacgtcagca
     2941 aatacccccc cgatacacaa tatctccaaa ctgtcaagcc aaaaatgaaa aataatgcct
     3001 gcacaaaata aagcgacccg ggaagattgc cgacaaattc acgcgcctcg gcaggattgc
     3061 tttccaatat cgaaccgact atctgataag acggcgcacc atacagccag ccgaccggca
     3121 aatatagggc ggttgtgccg acgtaaatca gtaaaacaac tgaagaaacg cgcgggaaac
     3181 cgcgtgccaa taataataaa aatacaaccg aagccaaagc acccacggca gaataacggt
     3241 agccgtattc atattccaag tgataacctg atgcgatggc cgcgccaaaa caaaaggcta
     3301 aaagcgcgca aatgcccaat gtatttttta atgtctgatc catatttttt attcgggcgg
     3361 attgtttcat acgatgccgt ccgaatatcc cattatttac gagttaacaa agcctgtccg
     3421 acgatttcaa gatcggaaaa acggtgcttc acgccttgta catcctgata gttttcatgc
     3481 cctttgccgg caatcaggat gatgtcgttt gcggcggctt gttcaaccgc ataacggatg
     3541 gcggcggcac ggtcggcttc gacgcattcg ggcgcgggaa cggcaggcag gatgtcgttg
     3601 atgatgtcgt gcggattttc caagcgcggg ttgtcgctgg tgacgacgac tttatccgcg
     3661 ccctgtacgg ctgccgcgcc catcagcggg cgtttgccgc gatcgcggtt accgccgcaa
     3721 ccgaatacgc accacaaagc cgcaccctgc ggtttgattt cctgcaaggt ggcgagtgct
     3781 ttttccaatg cgtcgggcgt gtgggcataa tcgacaacaa ccaaaggttt gccgctgttc
     3841 ataatacaat ccatacggcc tgaagcagga cggatttttt ccagtacatc caatacctta
     3901 tccagcggat agccgttggc gcaaagcaaa gcgacacagg cggcgagatt ttgcgcgttg
     3961 aaccgtccga gcaggcgcgt gtggcatttc ccttcgcccc acggggtttg gaatacggct
     4021 tctatgccgt ctgaagaggc ggtaaagtcg gtaatgcgga tgtcggcgtg ttcgctgaag
     4081 ccgtaactgt aaacggccaa atcggggcag tcttttttca gacgacctgc gagttccgcg
     4141 ccgtattcgt catctacatt gataacggcg tgtttcaagc cgtgccagta aaacaggcgc
     4201 gacttgatgg caccgtaggc ttccatcgtg ccgtggtagt cgaggtggtc gcgggtgagg
     4261 ttggtaaaga ttgcgctgcg gaatgacacg ccgttgacgc gcgactggtc aagcccgtgg
     4321 ctggagactt ccatcgcggc gactgttgcg ccttgttgac ggaaacggta gagcagggtt
     4381 tggacatcga cgggggcggg tgtggtatgc gtggtttctt ccaatgcacc ccaaaagccg
     4441 ttgccgaccg tgccgacaat ggcggttttt tcgcccaaca aatcggcagc ttgcgccagc
     4501 cattgcgtga tggaggtttt gccgttggtt ccggttacgc cccagacttt gagaccgtct
     4561 gaaacgttgc cgtaaacttg cgccgccaat atgccggcac ggtgtttcaa atctttgatg
     4621 ccttgattgg ggactttcca ttcgggattc cacgcgaatt tgccatcgtc gtcccaaaaa
     4681 acaaaagccg caccgttggc aacggcggcg gggatataac tgcgtccgtc ggcatattcg
     4741 cccggacagg caacgaaaat atcgccttgt ttgatttggc ggctgtctga atgcaacaaa
     4801 cgccctgccg cgtttgcaca cgacagggtc gggatgccgg tttcagccaa aggggttaac
     4861 ttgctgaaca taaaacaatc tcgttgatac tcggattaag acggtgtttt gacggctgcg
     4921 gcggtcagtg gcttggtcgg ggaaatgccc aagatgttca ggctgccgcc cataattttt
     4981 ttgaagggcg gccctgccac tacgccgccg taataaccgt gggcagtcgg ttcgtcaatg
     5041 gttaccgcca caatcacacg ggggtttttg gcgggggcaa aaccgataaa ggtagcgatg
     5101 tgtttgttgt cggcataacg cccgttgacg aacttgcgcg ccgtgccggt tttcgcgccg
     5161 acatcgaaac cgtccaccgc acccgccgta ccggtgccgc ccggctcggt cacggaaacc
     5221 atcagattgc gtacctcgcg cgcggtcgat tctttgaata tgcgtttgcc ttgcggcgca
     5281 accgcctgtt tttcaaagct gaccggcagc aaaacgccgt cgtgcgtcag tgcggtatag
     5341 gcgcgcgcca attgcagcag gctcaattgc aggccgtaac cgaaagacat cgtcgcctgt
     5401 tcgatgggcc gccacctgcg ccaatttctc aacaaacctg cagtttcgcc cggaaagccc
     5461 gagtgcatac gcacaccgat gcccaactca tgatagaagt catacatttc ttcggcaccg
     5521 aaacgcgcag acagtttgct tgtgccgacg ttggacgatt tctgcatgat gccgcgcaca
     5581 tccaaagagg ggtaaacatg ggtatcgcgc acgggagacg gtccgatttt ataaggctgc
     5641 gtattcagcc gttcgttcaa atcggttttg cccgcatcca atgctttcgc aatcacaaac
     5701 ggtttgattg ccgaaccggg ttcgatcata tcggttacgg cacggttgcg ccgctgttcg
     5761 ctgtctgccc ggccgggcct gttgggatcg taggcgggcg tattggccaa ggcgaggatt
     5821 tcccccgtgc gggcatccaa aaccaccacc gttccggctt ttgcctgatg gtattcgacc
     5881 gccttgttca actcttcata ggccaaggtc tgaatcctct gatcgaggga aaggatgatg
     5941 tctttgccgt ttttcggggc tttattgcgc ggggagtcca agctgtccac aatattgccc
     6001 tgccggtccc gcaaaacgac ttccgcgccg tcttcgccat gcaggctgtc ttcaagcgaa
     6061 agttccaaac cttcctgacc tttgccgtca atatcggtaa atccgatgac gtgtgcaaac
     6121 aggttgccca tcgggtaatg gcgttttaat tctttttcaa atacaaagtt ttccaaaccc
     6181 aaggctttga cctcttcggc aaccttggga tcgagctgcc gcttaatcca gataaacgac
     6241 ttgcctttct gttcgagctt gttcctcaaa acatcaaccg gcacatcgac aagctcggac
     6301 aggcgttcca attgtgcggc agacggcatt tccttcatct ctttaggcac ggcaaacagg
     6361 gactccgtcg gcgcactcaa cgccaaaacc gcaccgttcc ggtccgaaac cgtaccgcgt
     6421 gtagccggca atgtttgagt ccgcacaatc cggttgtcgc cctgttcttt caaaaagtta
     6481 tacgttaccg tctgcagata cagtccgcgg gcaatcagac cggcaaacaa gaccgctatt
     6541 gccatcagga cgaagctgat ccgtccgtta ctggtcatcg gctttttgac ctgctcttct
     6601 ttgggcagca tccggggctt atattcactc ttaatcaaca tttttacttc tcgttattat
     6661 tatcctgacg caggaatccg attccggcac acaggctgct tctatctttg atgcgccacc
     6721 ataaaggtat tgcccgaaac cggcggatgg aggttttgtt tttctgccgc cgccctgatc
     6781 gcttcgtggt tcgccaaacg cgcctgttgc agcctcattt gcgcataatc ctgctccaag
     6841 gcgatttcct gttttttcgc cttatccaaa gctgtgaagt tgagcctgta ctggttttgc
     6901 tgcatcacaa cggaaaaagc ggaaacgcac accgcaagca gcagaaggaa attcaatttg
     6961 ttcattgcca ttcagacgtt tttctctgtg attgttccgg tatcggaccg gcagtccgct
     7021 ccgccacacg caaaaccgca cttctcgccc tcggattggc ggcaatttcc gcctcacccg
     7081 gctttaatgc cctgcccgcg attttcaggg gcggctcggg caaatccgct tccctgaccg
     7141 ccgcccagcg cggcaggggc gcgtgttgcg aatatttttt gacaaactgc ttcacaatgc
     7201 ggtcttccaa cgaatggaaa gcaatgaccg ccaaacgtcc gccctctttc agacgacaca
     7261 tgacctgcgg caatactgcc cctacttctt caagctcgcg gttaataaag atgcggattg
     7321 cctggaaggt gcgcgtcgca ggatcctgcc cccgctcgcg agtacggacg ttttgtgcca
     7381 cgatctgcgc cagcttgcgg gttgtatcga ttggactttc cgcccgttgc gcaacaatgg
     7441 cgcgcgcaat ctggcggcta aaccgctctt caccataatt cttgattacc tcgtgcaaat
     7501 cctgttccga cgcaaccgct atccactctg cggcagacat accgcgcgtc gtatccatac
     7561 gcatatccaa aggggcatcg aaacggaagc tgaaaccgcg gctgccgtca tcgatttgcg
     7621 gggacgaaat ccccaaatca aacagcgcac cgtccacctt gccgataccc aaaccgtcca
     7681 atgccgtctg aaacgaagca aaaccgccat gcacgacacc gacccgtttg tccgaacgcg
     7741 ccagctcttc tgccacagca atcgcctgcg gatctttgtc gaaaacaatc aaccgccccg
     7801 catcgcccaa acgcgacaaa atcagccggg aatgccctcc cctgccgaac gtaccgtcca
     7861 catagacacc gtcttcgcgc acggcaagcg catccaccgc ctcattcagc aagaccgtga
     7921 tatgccggta actttctgct ccactcacaa ttgcaaatcc gtctgaccca actggaaggc
     7981 aagttcgtca ggatcgtcat ccaaagcctg aaccatctca gcctcccact gctcgcgacc
     8041 ccaaagctcc aaacggttgg cacgaccgac caaaacgact tcacggtcga aatccaccct
     8101 tttcctcagt ccggcaggaa gcagcacccg gccggcgctg tcccattcca aaatttccgc
     8161 gttatgcagc aaaagatttt gaaaccgccg caaaacaggg ttatccgcca cttttaagtt
     8221 taaaagttgc gccgcaacct tttcccactc cgcaacaggg tacatcaaca gcttgtgttt
     8281 cgactcgagc gttaccacta cggcaggcgt atagaggcgc gacagaatgt cacggaattt
     8341 ggcaggaaca gccaaccgcc ccttactgtc gatgcttaat tcgtgtgcgc cgccgaacat
     8401 gacatgtccc aagccgaaat caaaatcaca agggtaaaag agacactttg ccccacaatt
     8461 ccccaccaat cgacactata agaaatttta aacactcggt caaatcaggg catgaaaact
     8521 cattaacata tctgaaattt ttattccttt taaaaacaat aagataaaaa atgacgacaa
     8581 cggcacggcg ggtgcggtac agaataatcg aaccaataaa caactatata tgattaattt
     8641 aataatataa acacaatata tagtattaag ataaagccat gacagcaccc gcaccaacgt
     8701 gtaatatgtc gggaaatcca ataaatttac acaagctaac acttatcatg cccctccccc
     8761 tcccctctcc cgaagcacgg caattctcgc tcaaactgca aaccctcatt gccgaaaaaa
     8821 tcggcaaaca cggcaactgg attccatttt cacgttttat ggaattggtt ttatacgctc
     8881 cgcaatacgg ctactacacc ggcggcagcc ataaaatcgg caataccggg gattttatta
     8941 ccgcaccgac cctcacctct ctgtttgcac agacactggc acgccaactt caagaacttc
     9001 tatcccaaac ggcgggcaat atctatgaat tcggcgcggg aaccggacag cttgccgccg
     9061 atttgttggg cagcatttcg gacggcatca gccgttacta tattattgaa atatcgccgg
     9121 agctggcagc acgtcagaaa aacctgattc aagcacgcgc accggaagca tctcaaaaag
     9181 ttgtccactt gaccgcactt cccgaagcgt ttgacggcat catcatcggc aacgaagtac
     9241 tcgatgccat gcctgtcgaa atcgtccgta aaaatgaagg cggctcattc gagcatgtcg
     9301 gcgtttgcct agataatgac cgttttacct attcggcacg accgctgcac gacttacagc
     9361 tatctgcctt ggcttccctc tattttcctc aaacagatta tccctacacc agcgaactac
     9421 atccgcaaca atatgccttt atccgcaccc ttgcctcaag gcttgaacgc ggaggtatga
     9481 tattcatcga ctacggtttt gatgcagcgc agtattacca ccctcaacgc aatcaaggta
     9541 ctctgatcgg acactaccga catcacgtta tccacaatcc ttttgacttc atcgggttgg
     9601 ccgacctgac cgcgcatgtc aactttaccg acattgccca agccgggacg gatgccggat
     9661 tagatttgat aggttacctt ccccaatccc atttcttatt gaacctgggc attaccgagc
     9721 tattggcaca gacggggaaa acggattcgg cagcctatat ccgtgaagct gctgccgttc
     9781 agaaactgat tgaccagcat gaaatgggcg aactgtttaa agtcatcgca ttcggaaaaa
     9841 atatcggcgt cgactgggca ggattccgct tcggcgacat ctgccacaaa ctctaaccct
     9901 catgccgcct gaatccgctt cagacgacat aaacttttta acatttaaaa acagtcaact
     9961 aattcaaaat taaaaaatac ggcttgtcaa aaaaacagaa aaacatataa tagcgtcttc
    10021 acgaaacggc gaattagctc agtcggttag agcagaggaa tcataatcct tgtgtccggg
    10081 gttcgagtcc ctgattcgcc accaaatttt cgggggtata gctcagttgg tagagcgctt
    10141 gcatggcatg caagaggtca gcggttcgat tccgcttacc tccaccagat aaaaaagcac
    10201 agaccgtaaa aaggtatgtg cttttttatt gcctgattgc cagcaaataa agaataaacc
    10261 actgccttca aaacaggcaa tcgactttaa acctatcgcc ccgcctgtcc tgattttata
    10321 gtgaattaaa tttaaaccgg tacagcgttg gctcgccttg ccgtactatc tgcggcttcg
    10381 tcgccttgtc ctgatttttg ttaattcact atatcagccc gccagacaaa cccgacccga
    10441 ataatgtctt caggtcgggt ttatggtttc attcccaact tatccagcct gacagccaca
    10501 atataatgat ggcaatacca aaaacaatgc ggtaataggc aaaaggaata taatttttct
    10561 tggaaacaaa cctcagcaac gcttttaccg ctaccaagcc tgaaacaaag gcagcaataa
    10621 agcctatcag aatcaaaccg acatcatgca gggtgaaaaa tcggtaatgt ttcaggacat
    10681 cataagccgt tgcggcaacc atcatcggca cagccaagaa aaacgagaat tctgtcgcag
    10741 ttttccgttc gatgccccaa agcatcccgc ccataatcgt actgcccgaa cgggacgtac
    10801 ccggaaccag tgcaaacact tgggcaacgc cgatcatcaa ggcatcaatc ggacgcaatg
    10861 catcaacatc ggcaatttta ggctctgctc ggctttggcg tttctccacc cacaaaataa
    10921 aaaaaccgcc caaaaccagc atgactgcaa cactcaaggg gttaaacaga tactctttga
    10981 tttgtttgcc gaacaacagc cccatcacgg cggcaggtat aaaagcaatg gcaagattaa
    11041 ggacgaagcg gttggctttc cggtcttttc ccaagccgtg caacacattg ctgaaacgtt
    11101 gccggtattc aaacactacc gccaaaaccg caccgagctg gatggcaatt tcaaaaacct
    11161 tgtgattgct gtgaaaacca atcagattgc cgaacacaat caaatgtccg gtgctggaaa
    11221 tcggtaaaaa ttcggtaaaa ccttctacca agcccatcat cagggctttc aggacaatca
    11281 gaaaatccat tgcttgcgct tctttcggat acgggagttc ggctatttct gtacagcagg
    11341 ggtctgacgc ttgcgttctt ccctgacttt ggcaaccaat tctttaatcg tatgaacgcc
    11401 gccgtcaaag ccgttattga agataacgcg gtattttccg ccgacaataa cggtcggcgt
    11461 gctgtcgatg cggtattgtt ccgtcagttt ctgcattttt aatgcggcgg cggcagcttc
    11521 gggggaatca taggcgcgca tcagtttttt gccgtcaaag cctttttgag acaaagccca
    11581 ttttccggca acagccctgt tttccaagcg gattttttgt tcgtaaactg ctttaaacac
    11641 ggcagggttt gcctgatatt tcaaacccga caaattgacg gcagccgcca tcctggccag
    11701 accgagcatt tcaggctgcc agaccacgtg ctccgtcctc aaataggcat cagacggcaa
    11761 tgccttgccc agtttcaata acaaaggatc gaaatgatgg caatgtacgc agaaatagcc
    11821 gaaaaattcc aaaacctcaa ttttacccga ctgttcttga ggaatgggtt tatccaacac
    11881 aagatagtct tccccttccg tcagggcata tgcctgcgcg gacaacgctg ccgacagcag
    11941 cagcggcaac agatgtttga gcttcataat tatttacttt cgatagaacg gatcaggctg
    12001 gcgacttcat gttttttcaa ctcgtcctgc atttttttca ccgcatcggc agacatattg
    12061 ccgctttgca cccggtaaag cgttttatgt cccgcctgat aaccgaccac cttggaagat
    12121 atgcccaaga ttgccagttt ggcacgctgt ccttccgcgc tccggcggtc ggcatacgcg
    12181 cccatttgca gataatgcgt tgcttccgcc ttatcgggcg ttttcatttt ctgcacttct
    12241 ttggcagcgg cactgcgcgc tttttcgatg ctgccgctgt tgaggatttg ttccggggtc
    12301 ggtttgggtg caactttttc cttctccgcc tttttctctt cttttgaagc ttttttctct
    12361 gtttctttag atggttttac cgcttgtttt ttaaccgttt cggcatcttt cttctgcgct
    12421 ttttccccga cggtttgttc acgctcttcc gtcagtgctt tcttgcgcac tgcctgtccg
    12481 tccgactttt cccgctccgg ctcgtccgcc ttttcctcaa cctcgtcggc tttgtcggca
    12541 acgggctgct tgtcggcagc tttttccgca tccgactgct ctgcctcttt cgcagcatcc
    12601 ggttcggaca aggcgttttg atcggccggt tcaggttgga tgtcttcctt aggctggttt
    12661 ttcggtttca ggatttccgt ttctgcaggc tgcttcgacg gaaccgggat tttgaacgca
    12721 ttttgaccgc tctggttcag ataaaacaaa ataccggcaa taatgaccgt cgccagtatc
    12781 aaaccgaaga aaaaaccgga cagacctttt ccggattggg aaaatttgtt cataaacata
    12841 ccttaatgtg tttcagacgg cattaacgcc gtttgcttac gggcggatat tctaacaata
    12901 tcgccatatt tgggcaaaac ctgcttccat tcccattcct ataaagcacg acggaaacct
    12961 atggctttca tgacgtggct tctgccgact tcttcgtcgc ccgccaaatc cgccaatgta
    13021 cgcgccacgc gcataatgcg gtggaagctg cgggcggaaa gggagagttt ttccagcagg
    13081 ccgcccaatg cttcctgcgc ttctttttga atgcgggctt gggaatcgag ttcgctgaca
    13141 ctcaaagcgg cgttgacttt gccctgccgc gcgtattgtt tgtcgcgggc ggcgataacg
    13201 cgttccaaaa cggacgcgct gctttcaccc gcttcctgct gcatcagttc ggcggcggac
    13261 aggctgggga cttcgatggt caaatcgatg cggtcgagca gcgggccgga aattttgctg
    13321 cggtaacgcg cgacgctttc gggcgtgcag cggcagggtt tgacgggatg cccgagataa
    13381 ccgcacgggc aggggttcat ggcggcaaca agttggaatt tggcaggata gacggcttgg
    13441 cgcgccgcgc gggaaatgtg gatttcgccg ttttccaacg gttcgcgcaa aacttccaaa
    13501 actttgcggt cgaactcggg cagctcgtcc aaaaacaata cgccgtggtg cgccaatgaa
    13561 atctcgcccg gacgcgggtc ggaaccgccg ccgaccatag ccgccgcgct ggcactgtga
    13621 tgcggactgc ggaaaggacg gtggctgtcg agttgttgct ggtggttggg caggagcgaa
    13681 cgcaatgccc aaacttcaac caattcgtct tcggtcagcg gcggcaggat gcctggcagc
    13741 cgttgggaga gcatagactt gcccgttccc ggcggaccca tcatcaagag gctgtgtccg
    13801 cctgcggcag cgatttccaa agcaaggcgg gcagtgtgct gacctttcac atcacacaaa
    13861 tcaggttgtc cgccatgttc agacggcatt tgcggtacgg aacattcggt ttgcgccaaa
    13921 ggttcgatac cgttcaaatg ggcggcgact tcgcccaaag agcgcgcgcc gtaaacggta
    13981 atgccgcgca tcacggcggc ttgtcctgcg ttttcttcag gcaaaacaaa tgcacgtttt
    14041 gcctgcatac cctgccacgc catcgccaac gcgccgcgca cggggcgcaa cagccccgac
    14101 agtgccaatt cccccgcaaa ctcgtattcc gccagttttt cgggcgcaac ctgccccgat
    14161 gcggcaagga tgccgattgc aatcggcaaa tcgaaacgcc ccgactcttt gggcaggtcg
    14221 gcgggggcga ggttgacggt gatttttttg gcggggaatt caaaaccgct ttgaataatg
    14281 gcggcacgga cgcggtcgcg actttctttg acttccgtat cgggcagtcc gacgatgttg
    14341 aaatgtggca ggccgttggc aaggtgggct tccacttcga ccaacggcgc attcataccg
    14401 ctcaaggcgc ggctgtaaac caaggcaagc gacatatttc agacggcctt attcgccggc
    14461 ttcggtttgc tgcctgattt cggcgacggc ttcttcggca gcggcttcag ccgcttccaa
    14521 tgctgcccgt tcgggatttt gcgcggcttc gagttttgcc aaacgcgctt ccaaagccgc
    14581 cagtttggta cgggttttga ttaaaacctg ctgctggatg tcgaattctt cgcgcgtaac
    14641 caaatccata cggttgaacg cgccgcccag catcgcctta atattttttt ccacatcttt
    14701 ggcagggctg ttgacgatgg tttcgctgat tttcgaaccg acttcttcaa aaagctgctt
    14761 gccgaacata atctgtatcc ttcctgaaca tatcaaattc aatcggctat tgtataagga
    14821 aaaatgccgt ctgaaaacgg gcggcggata atcggcaaaa cataccgcgc ctcctttgcg
    14881 gttgctaaat tacacctcaa aacacctgcc gcctgaaaac ggatttcata ttccgcgcaa
    14941 cgccagatta caagacacta caaaacaata tactgtttta aatgattttt ccgacgcgca
    15001 tcgtttcaaa ctcggctttt taagccatta agtgctttgc aaacaacaga aattgggtta
    15061 tgctgaaacg gattatttac aatttcatat agttttatta catatcttat tgtgattgaa
    15121 gataatttat ccgaatcccc ctttcgggta tccggatttt ccgttgtact tttatttaga
    15181 aaaacatttc aggcgcaagt tgcttgcaat ttcaaagcct gtaattctga agttttagac
    15241 gaggaatagc acatgaatcc catgtacatt acttttgcaa tctatctggt tgcagtcctt
    15301 ctcatcgggc ttgccgccta tttttctacg cgcaatttcg acgattatat tttgggcggg
    15361 cgcagcctag gcccgtttgt taccgcgatg tcggcaggcg cgtccgatat gtccggctgg
    15421 ctgctgatgg gtctgccggg cgcgatttat ttgagcggtt tgaatgaggc ttggattgcc
    15481 atcggcctct tggtcggcgc gtatttcaac tggctcttgg tggcgggccg cctgcgcgtg
    15541 cataccgaat acgccaacaa tgcgctgacg ctgccggatt atttcttcca ccgcttcggc
    15601 gcgggcggac acttgatgaa agtggtttcc gcactgatta tcctgttctt cttcacgatt
    15661 tattgcgcct cgggcattgt ggcgggcgca accctgttcc aaagcctgtt tgaaggtatg
    15721 acttacaatc aggcaatgtg gctgggcgcg ggcgcgacca tcgcctatac cttcttgggc
    15781 ggcttcttgg cggtcagctg gacggatacg ttgcaggctt ctttgatgat tttcgcgctg
    15841 attttaacgc cggtaatggt ctatctgggc ttgggcggcg cggaacagat gtctgccgcg
    15901 attcaaagcg tcgccgcagg cacgggcaaa gaatacggca gcctgtttgc cggtacgacc
    15961 gtcatcggca tcatttccac cgccgcatgg ggcttgggct atttcggaca gccgcacatt
    16021 ttggcgcgct ttatggcggc cgaaagcgcg aaatcgctgg tatccgcacg ccgcatcggc
    16081 atgacttgga tggcgttgtg ccttgcgggc gcggtggcgg tcggttattt cggcattgcg
    16141 tatttcggtg caaatcccga caaagtttct tctatgagcg gcaaccacga acgcatcttc
    16201 atcgcgcttt ccaccctgct gttcaaccct tggatcgccg gcatcatttt gagcgcgatt
    16261 ttggcggcgg tgatgtccac cctgtcctgc cagcttttgg tttgctccag cgcgattacc
    16321 gaagacttct acaaaggctt tttgcgtaaa aacgcgcaac agtcggaact ggtatgggtc
    16381 ggccgcctga tggtcttggc gattgccgtg atttccatcc tgattgcttc cgatccgaac
    16441 agcaaagtat tgggactggt gtcttatgcg tgggcaggtt tcggtgcggc gttcggcccg
    16501 attgtgattc tgtccgtatt gtggaaacgc atcactgcct acggcgcgtt gtcgggtatg
    16561 gttgcaggtg ccttaaccgt agtcgtatgg gcggaatgga taaaaaaacc ggctcaagca
    16621 gcaggcgaaa gcggcttgtt gacgatgtac gaaatcgtcc cgggctttat tgtttgctta
    16681 atcattgccg tgttggtttc cctgttcaac aaagaacctt cgcgcgaaat ccaaaaacgc
    16741 tttgaaaaag ccgacgcaga ttaccgcgcc gcccgataaa cccctttcag acggcacggc
    16801 atcccggttc gtgccgtctg aacccgtttc cgggtcaaac acaaataaac cttgcctgca
    16861 tacaggcctt atttgtgttt tacccgtttc attttcaaag aaacggaagc acctaccctg
    16921 cccgcatttg aagccttgta tttcatctgc ggatttatta cgatattacc gtattcaggc
    16981 cgcaccgatg ccgcctgccc cccgaaaaac tttggagaat ccaaaaaatg tttcattttg
    17041 catttccggc acaaactgcc ctgcgccaag cgataaccga tgcctaccgc cgtaatgaaa
    17101 tcgaagccgt acaggatatg ttgcaacgtg cacagatgag cgacgaagag cgcaacgccg
    17161 cctccgagct tgcccgccgt ttggttaccc aagtccgcgc cggccgcacc aaagccggcg
    17221 gcgtagatgc gctgatgcac gagttttcac tctccagcga agaaggcatc gcgctgatgt
    17281 gtctggcaga agccctgctg cgtatccccg acaacgccac gcgcgaccgc ctgattgccg
    17341 acaagatttc agacggcaac tggaaaagcc atttgaacaa cagcccttcc ctcttcgtca
    17401 acgccgccgc ctggggcctg ctgattaccg gcaaactgac cgccacaaac gacagacaaa
    17461 tgagttccgc actcagccgc ctgatcagca aaggcggcgc accgctcatc cgccaaggcg
    17521 taaattacgc catgcggctt ctgggcaaac agttcgtaac cggacagacc attgaagaag
    17581 ccctgcaaaa cggcaaagaa cgcgaaaaaa tgggctaccg cttctccttc gatatgttgg
    17641 gcgaagccgc ctacacccaa gccgatgccg accgctacta ccgcgactat gtcgaagcca
    17701 tccacgccat cggcaaagat gcggcaggac aaggcgttta cgaaggcaac ggcatttccg
    17761 tcaaactttc cgccatccat ccgcgctact cgcgcgccca acacggccgt gtgatgggcg
    17821 aactgttgcc gcgcctgaaa gagctgttcc ttttgggtaa aaaatacgat atcggtatca
    17881 acatcgatgc cgaagaagcc aaccgtctgg agctgtcttt ggatttgatg gaggctttgg
    17941 tttcagaccc tgacttggct ggctacaaag gtatcggttt cgttgtccaa gcctaccaaa
    18001 aacgttgtcc gttcgttatc gactacctga ttgacctagc ccgccgcaac aaccaaaaac
    18061 tgatgatccg cctcgtcaaa ggcgcgtatt gggacagcga aatcaaatgg gcgcaagtgg
    18121 acggcttgaa cggctatccg acctacaccc gcaaagtcca caccgacatc tcctacctcg
    18181 cctgcgcgcg caaactgctt tccgcgcaag acgcggtatt cccgcaattt gccacgcaca
    18241 acgcctacac tttgggcgcg atttaccaaa tgggcaaagg caaagacttt gaacaccaat
    18301 gcctgcacgg catgggcgaa accctgtacg atcaagtcgt cggcccgcaa aacttaggcc
    18361 gccgcgtgcg cgtgtacgcc ccggtcggca cacacgaaac cctgctcgcc tacttggtgc
    18421 gccgcctgtt ggaaaacggc gcgaactcgt ctttcgtgaa tcaaatcgtt gatgaaaaca
    18481 tcagcatcga ccgcctgatt aagagcccgt tcgacaccat cgccgaacaa ggcatccacc
    18541 tgcacaacgc cctgccgctg ccgcgcgatt tgtacggcaa atgccgtctg aactcgcaag
    18601 gcgtggactt gagcaacgaa aacgtattgc agcagcttca agaacagatg aacaaagccg
    18661 ccgcgcaaga cttccatgcc gcatccatcg tcaacggcaa agcccgcgat gtcggcgaag
    18721 cgcaaccgat taaaaaccct gccgaccacg acgacatcgt cggcacagtc agctttgccg
    18781 atgccgcgct tgcccaagaa gcggttggtg cagccgttgc cgcgttcccc gaatggagtg
    18841 cgacacctgc cgccgaacgc gccgcctgcc tgcgccgttt cgccgacctg ctcgaacagc
    18901 acaccccagc actgatgatg cttgccgtgc gcgaagcagg caaaacgctg aacaacgcca
    18961 ttgccgaagt gcgcgaagcc gtcgatttct gccgctacta cgcaaacgaa gccgaacata
    19021 ccctgcctca agacgcaaaa gccgtcggcg cgattgtcgc catcagcccg tggaacttcc
    19081 cgctcgccat ctttaccggc gaagtcgttt ccgcattggc ggcaggcaac accgtcatcg
    19141 ccaaacccgc cgaacaaacc agcctgattg ccggttatgc cgtttccctc atgcacgaag
    19201 ccggcatccc gacttccgcc ctgcaactcg tcctcggcgc aggcgacgtg ggtgcggcat
    19261 tgaccaacga tgcccgcatc ggcggcgtga tttttaccgg ctcgaccgaa gtggcgcgcc
    19321 tgatcaacaa agcccttgcc aaacgcggcg acaatcccgt cctgattgcc gaaaccggcg
    19381 gacaaaacgc catgattgtc gattccaccg cacttgccga gcaagtctgc gccgacgtat
    19441 tgaactccgc cttcgacagc gcgggacaac gctgctccgc cctgcgcatt ttgtgcgtcc
    19501 aagaagacgt tgccgaccgt atgctcgaca tgatcaaagg cgcgatggac gaactcgtcg
    19561 tcggcaaacc gattcagctc actaccgatg tcggccccgt catcgatgcc gaagcacagc
    19621 aaaacctgtt gaaccacatc aacaaaatga aaggtgttgc caagtcctac cacgaagtca
    19681 aaaccgccgc cgatgtcgat tccaaaaaat ccacgttcgt tcgccccatc ctgtttgaat
    19741 tgaacaacct caacgaactg caacgcgaag tcttcggtcc cgtcctgcac gtcgtccgct
    19801 accgcgccga cgaactcgac aacgtcatcg accaaatcaa cagcaaaggc tacgccctga
    19861 cccacggcgt acacagccgc atcgaaggca cggtacgcca catccgcagc cgcatcgaag
    19921 ccggcaacgt ttacgtcaac cgcaacatcg tcggcgcagt cgtcggcgta cagcccttcg
    19981 gcggacacgg tctgtccggc acaggcccca aagcaggcgg ttcgttctac ctgcaaaaac
    20041 tgacccgcgc cggcgaatgg gttgccccga ccctgagcca aatcggacag gcggacgaag
    20101 ccgcactcaa acgcctcgaa gcactggttc acaaactacc gttcaacgcc gaagagaaaa
    20161 aagccgcagc ggccgctttg ggacacgccc gcatccgcac cctgcgccgt gccgaaaccg
    20221 tccttaccgg accgaccggc gagcgcaaca gcatctcatg gcacgcgccc aaacgcgttt
    20281 ggatacacgg cggcagcacg gttcaagcct ttgccgcact gaccgaactt gccgcctccg
    20341 gcatacaggc agtggtcgaa cccgacagcc ccttggcttc ctacaccgcc gacttggaag
    20401 gtctgctgct ggtcaacggc aaacccgaaa ccgccggcat cagccacgtt gccgccctgt
    20461 cgcctttgga cagcgcacgc aaacaggaac ttgccgccca cgacggcgca ctcatccgca
    20521 tcctcccttc ggaaaacgga ctcgacatcc tgcaagtgtt tgaagaaatc tcttgcagcg
    20581 tcaacaccac agccgccggc ggcaacgcca gcctgatggc ggtcgccgac tgattttacc
    20641 gaaatccccg ggcgcgggcc gtgaaccaat gccgtctgaa aacctttcag acggca
//
LOCUS       DAXHPG010000018        20625 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXHPG010000018 DAXHPG010000000
VERSION     DAXHPG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20625
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.018"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            99..740
                     /locus_tag="ACFM6F_000487"
     CDS             99..740
                     /locus_tag="ACFM6F_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC6163961.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            1077..1583
                     /locus_tag="ACFM6F_000488"
     CDS             1077..1583
                     /locus_tag="ACFM6F_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163962.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFHYIVCNLSAISSFCLM
                     KVIHSYTILCLLIMIIGIAFGFILRNRVLSIVKNKQNFLSDMFPLEMPFFSRVYKIPI
                     LARFLNRGMKWLYLQNFFCLVMICFSISLILNEERGWMPYFACYWFGLCYPVSTYYVF
                     LSRDYKHI"
     gene            1719..2519
                     /locus_tag="ACFM6F_000489"
     CDS             1719..2519
                     /locus_tag="ACFM6F_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFC6163963.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(2971..3609)
                     /locus_tag="ACFM6F_000490"
     CDS             complement(2971..3609)
                     /locus_tag="ACFM6F_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC6163964.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            3864..4706
                     /locus_tag="ACFM6F_000491"
     CDS             3864..4706
                     /locus_tag="ACFM6F_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC6163965.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVERPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGIKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            4772..5434
                     /gene="exbB"
                     /locus_tag="ACFM6F_000492"
     CDS             4772..5434
                     /gene="exbB"
                     /locus_tag="ACFM6F_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC6163966.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            5455..5889
                     /locus_tag="ACFM6F_000493"
     CDS             5455..5889
                     /locus_tag="ACFM6F_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC6163967.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(6380..6808)
                     /locus_tag="ACFM6F_000494"
     CDS             complement(6380..6808)
                     /locus_tag="ACFM6F_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC6163968.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(6873..8621)
                     /locus_tag="ACFM6F_000495"
     CDS             complement(6873..8621)
                     /locus_tag="ACFM6F_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC6163969.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHADSTALLADRHSLPPFGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTIMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTENLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            8948..10381
                     /gene="ccoN"
                     /locus_tag="ACFM6F_000496"
     CDS             8948..10381
                     /gene="ccoN"
                     /locus_tag="ACFM6F_000496"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC6163970.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            10408..11019
                     /gene="ccoO"
                     /locus_tag="ACFM6F_000497"
     CDS             10408..11019
                     /gene="ccoO"
                     /locus_tag="ACFM6F_000497"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC6163971.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            11024..11194
                     /locus_tag="ACFM6F_000498"
     CDS             11024..11194
                     /locus_tag="ACFM6F_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC6163972.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            11218..12096
                     /gene="ccoP"
                     /locus_tag="ACFM6F_000499"
                     /pseudo
     CDS             11218..12096
                     /gene="ccoP"
                     /locus_tag="ACFM6F_000499"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            12205..12553
                     /locus_tag="ACFM6F_000500"
                     /pseudo
     CDS             12205..12553
                     /locus_tag="ACFM6F_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
     gene            12755..14164
                     /locus_tag="ACFM6F_000501"
     CDS             12755..14164
                     /locus_tag="ACFM6F_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC6163973.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            14266..17475
                     /gene="recC"
                     /locus_tag="ACFM6F_000502"
     CDS             14266..17475
                     /gene="recC"
                     /locus_tag="ACFM6F_000502"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC6163974.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGSRDTLLHALQTDIQNLDMPSEKAGNINTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYTLECLSDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWQQPATVGEWVARCRDLLETLFQTEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGQDFSQTAARIRAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAEMRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTFAAAEKKEEAGAA"
     gene            complement(17545..19113)
                     /gene="mtrF"
                     /locus_tag="ACFM6F_000503"
     CDS             complement(17545..19113)
                     /gene="mtrF"
                     /locus_tag="ACFM6F_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC6163975.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            19445..19657
                     /locus_tag="ACFM6F_000504"
     CDS             19445..19657
                     /locus_tag="ACFM6F_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6163976.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYENIARDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
     gene            complement(19750..20382)
                     /gene="mtrR"
                     /locus_tag="ACFM6F_000505"
     CDS             complement(19750..20382)
                     /gene="mtrR"
                     /locus_tag="ACFM6F_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC6163977.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT         5306 a         4720 c         5374 g         5225 t
ORIGIN      
        1 ttatttattt acggtgcata ggcgggaaga taaacttgtt tcgggtggcg cggggtgtta
       61 aggtagccgg taccgacagg ttgacgagga atattttgat gaaactgtat atttacgacc
      121 attgcccgtt ttgcgtgcgt gcgcggatgg ctgccgggct gtttggcgcg gacgttgaag
      181 aagccgtgct ggcaaacgac gacgaggcaa cgccgatcgg tatgatcggt gcgaaacaag
      241 tgcccgtcct gcaaaaagaa gacggttcgt ttatgggcga gagtttggac attgtgcggc
      301 atttcgaccg cgaagaccgt ctgaaagacg aagtccgccc cgagatacgg gcgtggctgg
      361 acaaggtggg cggatacaac gacaaactgg tgcagccgcg cgtgatcaaa atcgggctgc
      421 ctgaatttgc aacgccggaa gcggtgaaat attttacaga caaaaaagag aaaagcatcg
      481 gcagcttttc cgccaatttg aataaaaccg cccaatacct cgaacggata aacgcagatt
      541 tgcaagagtt ggaaaatttg atggacggtg cttcagacgg catcaacggc ggaatcggta
      601 tggaggatat tttggttttc cccgttttga ggaatctgac cgtggtgcgc ggcatcgcgt
      661 tccctcggaa gacgatggat tatttaatcg gtatgagtga aaaatcgggc gtgccacttt
      721 atttcgacag ggcattgtaa aagtggctgt tattaactga cagatgaagg aggttctacc
      781 ctatttgagg taaactctat ttaccattgg ccgaaatacc agtgcttatc tccaagacga
      841 ccttcccaat cccttttgcc tcttaaaaag aagtgactag tgtgtaaagt tatagttcct
      901 tttatagctt tccaataaaa ttaaagtatt taaaagcttg tttagttcta atttgttaat
      961 ttctgtatct aaataatgtt cagaatagta ttatttttta aataaaccat gaatagtact
     1021 tcacattgtt tgtcgtatta tttagctttt aaatttctaa tgtgaaagat agtttaatgg
     1081 acgcctttga tttttttatg caaggcaatt ttaaaaaaat tgcaactgaa caaggtttag
     1141 accgtaaata ttttatattc cactatatcg tatgtaattt atctgccatt tcttcttttt
     1201 gtttaatgaa ggttatccat agttatacta tcctttgttt attaataatg ataattggca
     1261 ttgcatttgg atttatcttg agaaataggg ttctatctat agtaaaaaat aaacaaaatt
     1321 ttttatctga tatgtttccc ttggaaatgc ctttcttttc tagagtatac aaaataccta
     1381 tcttggccag attcttgaat aggggaatga aatggcttta tctccaaaat tttttctgtt
     1441 tagttatgat ttgtttctcg atatctctta tccttaatga agaaaggggg tggatgcctt
     1501 attttgcctg ctattggttt ggactttgtt atccagtaag cacttattac gtttttttat
     1561 ccagagatta taaacatatc taaactatta tgcgtggcgg tacttccaaa ggtaaatttt
     1621 aacctaatca atttactcaa accatccaaa aattaccaag cagcaatata aacacagatt
     1681 tatttgagcc aacctgatca aaacaaggaa ctaaatagat gaaaaagacc atttttaaca
     1741 ttaccaaaat ggactgcccg tccgaagaac agctcattcg catgcgttta aaagatgtgt
     1801 ctgatattta tgaattacag tttgatattg ctgggcggtg cttaaccgtc tatcacgata
     1861 atcaagatac aacgatttta caagtacttg aaccgcttaa ttttgatagt catatcattt
     1921 ccaccgaagt gattgttgat aagatagtgt ttaacaagcc tgatgagcac ttagaaaaaa
     1981 gattattata tcaagtgctc atgattaact ttgtgttttt tatcatagag tgttcggttg
     2041 gtatttttgc taattctatg gggctgattg ctgatagtct agatatgctg gcggacagct
     2101 ttgtctatat attggcgcta tcagccattg gtatgacgct ggcgtataaa aaacgtgtgg
     2161 catttttggc ggggataacg cagataatat tggcgttatt tggggtgata gaagtgatta
     2221 ggcgttttat cggtgctgag cagctgccca attatcaact gatgattggc acggcatttt
     2281 tggcattgat tgcaaactgg ctgtgcttat atctactgag caaaaatcaa aataaagaaa
     2341 tccatatcaa agccagcatg atttttacat ccaatgacat tatcatcaat atcggtgtga
     2401 tagcggcggg agcattgacg ttattgactc attctagcta tcctgattta atcattggca
     2461 tgattgtctt tgtcattgta ctatttggtg ccagaaatat tttaaaattg gcaaaataat
     2521 tcctttcacc agcaaaggag tttttatgca aatatcggtc aaagctacct acatacgtgg
     2581 cagtacttcc aaaaacttga ttttttaaaa tgtggttaat gcgagtttgt ttatctggta
     2641 tttgattggc caagccttca tccaagcgtt tgacgatttc acgcaaaaat acccctgatt
     2701 tacacacagg gtctaagaat gtcgcctttg gatttgacca aatctcagac ggcaataaat
     2761 ccagcatttg attggcaagt tttggcgatg tgaaaacctc atcattagac agactggagt
     2821 ttcttaatca gtgtttcagt taatgaaagg tattatcatg caacttggtt ctaataatga
     2881 tgaaaaaaat aagattacac tacaggaaaa cgcggcaggg caatatgtcc tgccgcgttt
     2941 tctggagcgt ttatgccgtc tgaaagccag tcagacgaac atctgcacaa acgcaatcac
     3001 cagccgcaca atcggtgcaa taaacgcacc caaaaccccg gtcagcataa gcagcaggat
     3061 aatccacgtc ccataaggtt cgattttgcg gaacgcttgc gaatatttcg ccgacaggaa
     3121 ggtgtcgatg aaaatgccgc cgtcccaagg caggatgggg atgatgttga gcgcgaacag
     3181 aatcgcattg atcagaatac cgtagtttgc catttgagcc aacggcatct gatacgcccc
     3241 accgacatac ggagtcagca ccaaaaccac gccccacaga acagccatcg ccagattcga
     3301 cagcgggccg gacgcggcaa cgcaacgcca ggcaaggcgc gggttgcgga agttgcgcga
     3361 atcgatagga atcggacgcg cccagccgaa caggaagggc gtaaacatca aagtaagcag
     3421 cggtacgatg attgtgccga ccaaatcgat atggggcagg gggttcagtg tcagcctgcc
     3481 gtattgttcg gcagtgttgt ctccccagta gcgcgccgta tagccgcgcg ccacctccct
     3541 gacggtaatg gcgagcagca cgggcaggac ggcaagcaga aacacgccca aatcaaaatt
     3601 ttgaaacatg gttttccttt gaacagatgt ggtgtgcagc ccgaaacggc tgacagacag
     3661 aagcggcaat atatcatcta acctgatttt attggaataa gaaatcagac ggaatcattt
     3721 tttctgccgg cccgttatct gatgatttgc tgtaatacag cgtttaaaca cggtttgcct
     3781 gtgaacggaa cagacattca tatgaccaaa aattgtaaaa ataggttgca aataggaatt
     3841 gttgctatta ttatgcccga attatggata aagaacgaat tttaaccccc gcagtcgtgt
     3901 tttccgtcgc actgctgcat ttggcaatgg tggcattgct ctggcaggcg cacaagctgc
     3961 ccgtgataga gtcaggaaat gttatcgaat ttgtcgattt gggtgatttt ggcggagggg
     4021 acggtgcacc cgaaggcgca ggtgcgcctg ctgcgcctga gccgcaacct gagcctgagc
     4081 cgcccaaacc tgtcgagccg cccaagccgg ttttgaagcc ggtggttacg aaaaaggcgg
     4141 atgcggatat tcagcagcct aaggaagagc cgaaacctga agaaaagccg aaacccgaag
     4201 aaaaaccgaa accagagcct aagccggaag cgaagcctgt cccgaaaccg gcggaaaaac
     4261 cggtcgagag gccgtctgaa aaacctgccg aacatcccag caatgcttct gccaaagcag
     4321 acagcgagca gggtaatggg gaagataagg gaaccggtat caaaggagac ggaacggggc
     4381 gcggagaagg cagcggtaaa ggtagcggcg gtgtcaaagg cgaacacggg gaaggagccg
     4441 gcagcagcaa aggcaatcct ttacgcgcca atggcagcat tccgcgcccg gcttatccca
     4501 cgctttctat ggagaatgac gagcagggta cggttgtttt gagcgttttg gtttctccgg
     4561 gcggtcatgt cgagtccgtt aaaatcgtga aaagcagtgg tttttctcgg ttggacaatg
     4621 cggcacgcaa ggcggcgcaa aacgggcatt ttcaagccaa tgcctggacg gagtttaaag
     4681 tccccgtcaa gtttgaattg aattaggcgg ttgccgcagc ggtttgcggc agcttggatg
     4741 tttgattggt tttttcagtg ttggaatcat tatgaatttg aaattagtat ttgaatcggg
     4801 cgatcccgtc ctgatcggtg tgtttgtgtt gatgctgttg atgagtatcg taacgtggtg
     4861 tttggttgtc ttgcgctgca tcaagctgta tcgggcgcgc aaagggaatg ccgccgtcaa
     4921 acggcatatg cgcgatactt tgtcgctgaa cgacgcggtc gaaaaagtgc gcgccgtcga
     4981 tgcgcctttg tccaaactgg cgcaagaggc attgcagtct taccgcaact accgccgaaa
     5041 cgaagcgtcc gaactggcgc aggctttgcc gttgaacgag tatttggtca ttcaaatccg
     5101 caacagtatg gcgcagatta tgcgccggtt tgattacggg atgaccgcgc ttgcctccat
     5161 cggcgcgacc gcgccgttta tcgggctgtt cggcacggtt tgggggattt accacgccct
     5221 gatcaatatc gggcaaagcg ggcagatgag tattgcggcg gttgccggcc cgattggcga
     5281 ggcactggtg gcgacggcgg cgggtttgtt cgtggcgatt ccggcggtgt tggcatacaa
     5341 cttcctcaat cgcggcacaa aaatactgac ccaggatttg gatgcgatgg cgcacgattt
     5401 gcacgtccgc ctgcttaatc aaaaggatag ctgattataa aaggatagct gattatggca
     5461 tttggttcga tgaattccgg cgacgattct ccgatgtccg acatcaacgt tacgccgttg
     5521 gtggacgtga tgctggtgtt gctgattgtg tttatgatta ctatgccggt gctgacgcat
     5581 tccatccctt tggaactgcc gaccgcgtcc gagcagacaa acaagcagga caaacagcct
     5641 aaagaccccc tgcgcctgac gattgatgcg aacggcggct attatgtcgg cggggattct
     5701 gcaagcaaag tggaaatcgg ggaagtggaa agccgtctga aagccgccaa ggagcagaat
     5761 gaaaacgtga ttgtggcgat tgcggcagac aaggcggtgg aatacgatta tgtaaacaaa
     5821 gctttagaag ccgcccgtca ggcaggaatc accaaaatcg gttttgtaac cgaaaccaag
     5881 gcgcaataaa gttcgacgct tgaacgaatg ccgtctgaaa gtctttcaga cggcatttct
     5941 tatgtttaat cagctatata tagttgatta aaacaaaaat gagacgacag agcgccgtct
     6001 ccatttctgc gcaggcggga atacaaacat ctgatttttc ttttattttc aaacattact
     6061 gaaatgggta agtctggagt cctgcctgcg cgagaataat gaaaatgtgc atttctaatt
     6121 tttatagtgg attaacaaaa atcaggacaa ggcaacgaag ccgtagacag tacaaatagt
     6181 acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctcttt gagttaaggc
     6241 gaggcaacgc cgtactggtt tttgttaatc cgctataccc actgtaaaaa cggagaagga
     6301 cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt tgcggtggaa tgccgcttga
     6361 aggtgttttc ggcttgtaat cagacgcggg cgttgtccag ccgctctctg aaattttcca
     6421 aatttacgcc tgccgcttgg gcgcggcgga tgatttcgtc aaccggcata ccttctgccg
     6481 cccggcggaa gccccacagg agggagcagc gcgtaccggt ccggcaatag gcaaggacgg
     6541 gatattcggc ttgtccgatg agttggcgga aggtttcgac atcgtgtttt tggatgtcgc
     6601 gtgcggtaac gggttggtga tggaatccag taacgcctgc ttgttccagc cactgtttga
     6661 tttgggcgaa gtcgggttgc gattcttctt cgcggtcggg gcggttgcag atgacggttt
     6721 tgatgcccag ttgcgcgatt tgttccgcat cggctttggt cagttgcgga gaaatataga
     6781 ggtgttcgtc aagtttcaga atggccattt tgtttccttt actttgcggt tgtttttcag
     6841 acggcatttt gtgtgcaatg ccgtctgaaa gtttaaccga acaaccagtt gtccaacagg
     6901 ttccggtctg tgatatgtag gatggcagta tccgctgccg ctgcctgaac cgtcaagacg
     6961 gtttgacgca atatgccggc acggaagaag tggatattga tttttgcatt gacgtggtat
     7021 cgggcccatt gtgcggtaaa gtcggtgcag gcataaccgt ctaaagcaat gattttgtct
     7081 tgcggacaca gtgccgcaga ttccgcgctg ccgccgttga agacatgggt caggacgatg
     7141 tggtcggcgt tttgtttgaa acgtgcgcca aaatcgcctg ccgacgggac ggggcagatg
     7201 tgttctgcgt atccgccgcc gtgttgtcgg ggaagcggca ggaaggtcag tcccacgcct
     7261 gcggttgcca ggcattcggc aagcggcaaa ttttcggtac tgtataacgc tttttggaaa
     7321 aaatcttcca aatccaagcc ggtaatttcc tgacagcgga tttgccagtg tttttccgga
     7381 atacccgagt gtgtgtccct ccactcccga tagagtttgt ccattatcgt atcgagagaa
     7441 tgtctgccgt tgcttcggtt gcgtattatc agatcaaggc acaatgcggc aagcgcgcct
     7501 ttctggtagt agctgacgat ggcgttgggg ctgttttcat caggtttgta aaatttgttc
     7561 cacgcggtaa aactcgattc cgccaaggtc tgcctcaaac ggccgcgggt ttgttgtacg
     7621 cgcgtaatgc cttgtgccag caggtttaaa taagattcgg gcgagatggt gcggctgcgt
     7681 gccaaaaaca aatcgtcgta ataggatgta atgccttcga atgcccatag ttgttcggta
     7741 tagttttctt tgtcgaggtc ataaggggcg aacgcggcag gtttgatgga tttgacgttc
     7801 cacgcgtgaa aatattcgtg ggagaaaagt ccgagcaatg tggtgtaggc atcgtcggca
     7861 tcggtcatac cgaacggcgg aaggctgtgg cggtcggcga gcagggcggt gctgtcggcg
     7921 tgttccaaac cgccgtaaat atggtcgccg acgtggagca ggaacaaata tttttgaaac
     7981 ggggcagggg aggaaaacac cgccagttct gtttcgcaga tttttttgat atccgaaacc
     8041 agcctgttgc ggtcgaaatc gggatagatg ccgcttaagg caattgtgtg cggaatgcct
     8101 gccgcctcaa aatctaaaaa ttcaatcaag cccatctcga caggtcggtc aatcaattcg
     8161 gcataagatg ccgcctgaaa gacaaacctc cctgtttccg gcagcgttgt ggcaatacgc
     8221 cattcggacg gaatacccgt caattccaag cggtgcggca gcgtttccgt tccttcgact
     8281 ttcaaaaaca ggcacgatcc gtcaaaaaaa ccgcgttctg tcgtcaggaa agaacctcga
     8341 accgacaaat cgaatgcata tacggtgtag cggatttgcc actcgccgcg tacggcggca
     8401 gcgtgccagc ggttttttgc aatttgttcg agcggcatgg acgtgccgtt acaggatgca
     8461 tggatagaag tgatgtggcg ggaaaaatcc cgaatcagat agctgcccgg aacccagttt
     8521 ggcaggctta tttctatagg aagatcatca tcttgtgtga atgtcagcag gatgtgccac
     8581 tcgtggtcga gaggggacgg ggttatttta taatgaatca taactatcaa atatatcgtt
     8641 aaacgggatg gattaaatac ctttaaaatg cagcttaacc tacatcaata aagtgccaca
     8701 aaaagggaaa attggaaaca aaagcaatgg cggtcgggtt gtaatttatt ataacaatat
     8761 gatttatatg taataaaaaa cagattaagc aaagggctcg ccggcggcgc gaatgtaatt
     8821 ccgcttggcg taataaggca gatatgttaa atttagaaaa aattacgctt gcccggattg
     8881 caccttaaac aggctgcgga agcggcagga taaaaatctt tattcccata aattcatgga
     8941 gactctcatg gacacacaaa cttacaacta caaagtggtg cgccagttcg ccatcatgac
     9001 tgtagtttgg gggattgtgg gtatgttggt cggcgttatc gtcgccgccc agctttttgc
     9061 tcctgccctt gacttgtcta atatcggacc ttggttccac ttcggccgcc tgcgtccgct
     9121 gcacaccaat gcggttattt tcgcatttgg cggttgcggc ctgatcggca catcatacta
     9181 cgttgtccaa cgtacttgta atacccgtct tttcggcggt tggctgccgg catttacctt
     9241 ttggggctgg caggcggtaa tcgttgccgc cgtcgtcagc ttccctatgg gttggaccca
     9301 aggtaaggaa tatgccgaac tggaatggcc gatcgatatt ctgattactt tggtttgggt
     9361 ggcttacgcc atcgtattct tcggtacgat tgccaaacgt aagattaaac atatttacgt
     9421 tgccaactgg ttctacggcg gctttatttt ggcggtcgca cttttacaca tcgtcaacaa
     9481 tatcagcatc ccggcagggt tgatgaagtc ataccccgtc tattccggtg cgattgatgc
     9541 tatggttcaa tggtggtacg ggcataacgc cgtgggcttc ttcctgactg ccggcttctt
     9601 gggtatgatg tactatttcg tacccaaaca agcagcccgc cccgtttact cctaccgcct
     9661 gtccgtcgtt cacttctggg cgttgatttt tacctatatg tgggcgggtc cgcaccatct
     9721 tcactacact gcgctgcctg actggacgca atctttgggt atggttctgt ctttgattct
     9781 gttcgcaccc tcttggggcg gtatgattaa cggcatcatg accttgtccg gcgcgtggga
     9841 caaactgcgt acagacccga ttcttaaatt cctgattgta tccttgtcct tctacggtat
     9901 gtctaccttt gaaggcccga tgatgtcgat taaaacggtc aatgcattga gccactatac
     9961 ggactggacc gtcgcgcacg ttcatgcggg tgcgttgggc tgggtaggct ttgtaaccat
    10021 cggttccgtc tattacatga ttccccgtct gttcggcaaa gaacagatgc acagcaccaa
    10081 gctggtagaa gcacattttt ggattgcgac catcggcgtg gttctgtata tcgctgccat
    10141 gtggattgcc ggtgtgatgc agggtttgat gtggagttct ttgaacgatg acggtacgct
    10201 gacctactcg tttgtcgaat ccgtaaaacg caccatgcct tactacgtga tccgttttgc
    10261 aggtggttta ttgtatctga gtggtatgtg cattatggcg tacaacgtgt accgcacagc
    10321 catcggtggt aaagcagtcg atgccgaaat ccctgcggtt tcccaaacac agcaccacta
    10381 aaatactaag aaagataggc taccaaaatg aaattacaac aattggctga agaaaaaatc
    10441 ggcgttctga ttgtgttcac gctgcttgta gtcagtgtcg gtctgttgat tgaagttgtg
    10501 cccttggcct ttaccaaggc ggcaacacag ccggcgccgg gcgtgaagcc ttacaatgcc
    10561 ttgcaggttg ccggacgcga tatttacatc cgtgagggct gttacaactg ccactctcaa
    10621 atgattcgtc cgttccgtgc ggaaaccgag cgttacggtc attactctgt tgccggagag
    10681 tcggtttacg accatccgtt ccaatggggt tccaaacgta ccggtcctga tttggcacgt
    10741 gtgggcggtc gctattccga cgaatggcac cgtatccacc tgctgaatcc ccgtgatgtc
    10801 gtgcctgagt ccaatatgcc ggcattcccg tggcttgcac gcaataaagt cgatgtcgat
    10861 gcaaccgttg ccaacatgaa ggctttgcgt aaagtaggta ctccttacag tgatgaggaa
    10921 attgcgaaag cacctgaggc tttggcaaac aaatccgagc tggatgctgt agtcgcctat
    10981 ctgcaaggat tgggtctggc tttgaaaaac gtaaggtaac atcatggata ttaacggtat
    11041 tcgtgctctc ttcacggtat ggatctttat ctgtttcctg ttggtactct atatcgtctt
    11101 caacaggcgg aataagaaaa actacgataa tgccgcaaac agcatttttg atgaaaacca
    11161 agatgcgcaa gataagaaaa gcgaaaaccg ttaatattgt gataacggag caaaacaatg
    11221 aacacaacat cccaatttac tagtaatttc tggaatatat atattgcagt tattgtctta
    11281 ctgagcttta tcgctttggc ttggctgctg ctgtctcaaa atgttgtcaa acgtccgaag
    11341 aagggcgaag aagtacaaac tacgggtcat gagtgggacg gcattgccga atacgacaat
    11401 ccgctgcccc gctggtggtt ttggctgtgt gttttgacgt ggctgttcgg tatcggttat
    11461 ttggttatgt atccgggtgt cggcgactac aaaggtctgc tgaaatggac cagccataac
    11521 caatatgaaa aagaggtcaa aaaagccgat gagcaatacg gcaaactgta tgccaagttt
    11581 gcggatatgc cgattgaaaa agtggcaaaa gaccctcaag ccaagcaaat cgcccaaaac
    11641 ctgtttaaca cttattgtat ccagtgccac ggctctgatg ctaaaggctc taaaggtttt
    11701 ccgaatctga ccgatagcga ttggttgtgg ggcggtgatc ccgataaaat tcacgagacc
    11761 atcgaaaaag gccgtgttgc gactatgcct gcctggggcc ctgctttggg cgaagaaggc
    11821 gtgaaagacg ttgcccatta tgtgatgtcc ctttcaaaac ccgaaggtca gtatgatgag
    11881 gaacgtgccg cgcgcggaca agccttgttc agcggtccgc ctgccaactg tttcacttgc
    11941 cacggcgata agggacaagg tatccaaggt ttgggtccga acctgactga tgacgtgtgg
    12001 ttgtggggcg gtacgcaaaa atccattatc gaaaccatta ccaacggtcg cagcagccaa
    12061 atgcccgctt ggggacattt cttggataaa gacaaactgc atattatgac tgcttatgta
    12121 tggggtcttt ccgataaaga cggtaaagct ctggtgaaaa aagccgagcc tgcaccgact
    12181 cccgcaccgg cggcagaacc cgcagcctct gctcctgcag aagcagcaca agccgcgtcc
    12241 gaagccaaac ctgccgcagc agaacctaaa gccgaggaaa aagccgcacc tgctgccaaa
    12301 gcggacggca aataggttta tgaaaccgtt tgtgccgcct gccatggcaa tgcggttccg
    12361 ggtattcccc atgtaggcat caaagccgat tgggccgacc gcatcaaaaa aggcaaggac
    12421 acgttcgttg cacaaacacg cgatcgaagg tttcaatacg atgcccgcaa aaggcggtcg
    12481 cggcgatttg agcgatgatg aagtgaaagc tgcggttgac tatatggtca accagtcagg
    12541 cggcaaattc taacttgact gagtttccga tattatcggt tatgaaaatc cagattcagt
    12601 attgaatctg gattttttgt tttaacgggc atccggtttt aaacgcagac tgcctatttg
    12661 ttgtaatgaa ttttgacttt agtcataaag aattagtctt tattgtttat aatcgcgcat
    12721 catcacgata gccctatttt caaaaggacg attaatggat acacaaatca aaactgaagc
    12781 cgacaatcag agcaaccggc gttatctgac cgtttggcgg tggcattttt atgccggtct
    12841 gctggttatg ccttttctta ccctgcttgc cgttacgggt ttgggtatgc tgctgtttgc
    12901 caatattacc ggtaaggagg gcgagcggat tcatgttgtg ccgcaggcaa cggtacaacc
    12961 tctgtctgtt caggcggaag cggcacgcag tgccgttaat ccggaaactt cgtccgtcgt
    13021 tcagtatatt gcgccgcgtg ccgatgatat ggttgccgtg ttccgtgtca acaatgaggg
    13081 caaggcaacg atggtcgcgg tcgatcctta tacggcaaaa gtggtcagta ccatgccgcg
    13141 caatcagggt tggtattacg cgatggatga aatccacagc gatatgatgc tcggttcgac
    13201 aggtgattat cttttggaaa cggctgcatc gctgacgatt atcatgataa tcagcggttt
    13261 gtacctttgg tgggtgaaac ggcgcggcat caaggcgatg ctgctgccgc caaaaggcag
    13321 ggcgcgttct tggtggcgga atctgcacgg cgcgtttgga acttgggtgt cgttgatttt
    13381 actgttgttc tgcctgtcgg gtattgcttg ggcaggtatt tggggcggca agttcgtgca
    13441 ggcttggagt cagttcccgg ccggcaaatg gggtgtcgaa ccgaaccctg tttcagtcgt
    13501 gccgacccac ggcgaggtat tgaatgacgg caaggttaag gaagtgccgt gggttttgga
    13561 gcttacgcct atgcctgttt cagggacgac tgtgggcaaa gacggtatta accctgacga
    13621 gccgatgaca ttggaaaccg tcgaccgttt tgcgcgggaa atcggtttca aagggcgtta
    13681 tcagttgaat ttgcccaaag gcgaggacgg agtgtggact ttgtcgcagg attctatgag
    13741 ttacgacatg atcagcccgt ttgccgaccg cacggtgcat atcgaccagt acagcggcaa
    13801 aatccttgcc gacatccgct ttgacgatta caacccgttc ggcaaattta tggcggcaag
    13861 cattgcgctg catatgggta ctttgggctg gtggagcgtg ttggcgaacg ttttgttctg
    13921 ccttgccgtc atttttatcg gtatcagcgg ctgcgtgatg tggtggaaac gccgtccgac
    13981 cggagcggtg ggcatcgttc cgccggcgca gaaagtcaag ctgccggttt ggtggatgat
    14041 ggcattgccg ctattggcaa tcgcactgct cttcccgacc gcactgcttg ccattgccgt
    14101 gatttggctg ttggatacct tgctgctgtc gcggattcct gttttgagga gatggtttaa
    14161 atgacccgat gccgtctgaa gcgttcagac ggcattttgt ttgaaggcgg gcgggggaaa
    14221 ggctatataa tcccgaatac ttgaccacag cttctgtttg aaatcatgtt ttatctgtat
    14281 caatccaacc gtcttgaaac gctggcggca ctgtttgccc gcattcaaaa agtcaaaccg
    14341 ctgaaatcgg ttttacagcc cgaacagatt attgtgcaga gtcaggggat gcgccgctac
    14401 ctcaatacct gcctcgcccg cgatttgggc gtggcggcga atttgtcgtt cagcctgccc
    14461 gccggtctga cgtggaagct gatgaaaaaa ctgattcccg gtattccgga actcagcccg
    14521 tttgcgcctg aagtgatgcg ctggcggctg ttggatttgt tccgcagcga ggcatttcgg
    14581 aatacggcag aatttgaaga tgtgaggaat gtgctgcaag actatctggg cagcggcgaa
    14641 tcggcggatt atcagcttgc tggccagctt gcagacatat ttgaccaata cctcgtgtat
    14701 cgtccgcagt ggatagacgc gtggcagcag ggcaggatac tcagtttggg cgacgatgaa
    14761 atctggcagt ccaaactgtg gcgttatctt gatgacggca ggcagagcgc gccgcaccgt
    14821 gttgcgttgt gggaaaagct gttggaatct ttgagcagtg ataagctgcc cgagcgttat
    14881 ttcgtgttcg gcatttccac gatggcgccg atgtatttgc agcttttgca caagctgtcc
    14941 gaacattgcg atgtgttcgt gtttgcactc aatccgagtg ggatgtattg gggcaacgtc
    15001 atcgaagcgg cgcaaatcct caaaggcggc ggcgatcccg atttaactca ggcagggcat
    15061 ccgctgctcg cctcattggg caagcagggg cgcgactttt tcgacttttt gaacgaaatg
    15121 gaaatagaag gagaaacgcc ggtgtttgaa gaaggcagcc gcgatacgct tttgcacgcc
    15181 ctgcaaaccg atatccaaaa cttggatatg ccgtctgaaa aagcgggaaa tattaatacg
    15241 gacgacggat cgatacgcat cgtctccgca cacagccctt tgcgcgaatt gcagatactc
    15301 aaagacaaac tgttgaaaat tctgcatgaa catcccgatt ggcagccgca tgatatagcc
    15361 gtattaaccc cgaacatcga atcctatacg ccttttatcg aagccgtgtt cggacaggcg
    15421 cagcccggtg cgcaggcatt gccgtattcc gtttcagacg tgaaaatcag ccgccgacaa
    15481 ccgttttttt atacattgga atgcctgtcc gacttgttgg aaagccgatt tgaagtcgat
    15541 aaagtgcttg tgcttttaga aaccgcaccc gtgttgcgcc gtttcggact gactgaggac
    15601 gatttgccgc ttttgcacga catggttgcc gatttgaacg tccgctgggg tttggacgga
    15661 gaaatgcgcg gcggcacgga tcagctgttt acctggaagc aggcggtaga acgcatgata
    15721 ttgggctgga tgctgcccga aggcggcaat ccgatgtggc aggatgtcag cgcgtggtat
    15781 gccgacgtga accaaaccgc cctgttcgga cgttttgccg ccctcctcga aaccctgtcg
    15841 gatattgtac ggatatggca gcaacctgcg acggtcggcg aatgggtggc gcgttgccgg
    15901 gatttgcttg aaacattgtt ccaaaccgaa gccgatgacc aaaaggcagt ccaaaacctc
    15961 gaaaacgaat gggtcaaatg gcaggcggaa accgaattgg cgcaattttc cggacagttg
    16021 ccgccgcaca ccgtcatccg ccacatccgc cgctttctcg acagcgaaag cgaggcaggc
    16081 tttttacgcg gcggcatcac cttttgcagt atggtgccga tgcggagcct gccctttaaa
    16141 gtcatctgtc tgttgggttt gaacgacgga gattttcccc gcaataccaa agccgccgta
    16201 ttcgacctga ttgccaaaca tcccgccaaa ggcgaccgcg cccgccgcga tgacgaccgc
    16261 tacctgttcc tcgaagccct catcagcgcg cgtgaaatcc tctacctgtc ctacatcggg
    16321 cgcgacatcc gcaaagacga agagcttgcc ccgtcttccc tgttgggcga actcatcgat
    16381 accgttgccg ctatggcggg catcggtagt cgccaacttg cacaaaactg gatagaacag
    16441 catccgctgc aagccttttc acgccgatat ttccaagaag gcggacgttc agacggcata
    16501 ttcggcacgc gtaccgacta cgccaccgcg ctcggacaaa cgcccgaacc gccgcaaccc
    16561 tttttcgacc aacccgtaga aaacgccgaa cctgttgccg aaatcggaca ggacgaattt
    16621 atccgtttct ggcgcaaccc cgtcaaagta tggcttcagc agcagcttgc gtggagcgaa
    16681 ccccatatcg gcgaagcctg ggagcctgcc gaacccttcg agccgcaaca cgccgatcaa
    16741 atcgccgaaa tctatattga ggcgcgccgc gaaggacaag atttttccca aaccgccgcc
    16801 cgcatcaggg cggaaagcct gctgccgtcg ggagagttgg gcagactttg gcagcaggac
    16861 ttccaaaccg ctgccaaaca aatcgacacg gcggttttaa acagtcccaa acttcctccg
    16921 ctgccctacg ccataccgtc ggacgggcaa atcctgaaag gcagcttggg caatctgtac
    16981 cgctgcggac aagtgtttta cgcctacggc aaacccaacg cgccgcaacg tatcgctttt
    17041 ttgttggaac acctgatatt ttgcgccgtt atgccgtctg aagccgaaat gcggcaaacc
    17101 tttatcgtcc aatccggaga aaccgaaata ttggcggaaa tcgcgcaaga cagggcattg
    17161 cagctattgt cggaatggat ggcgtttttc aatatcgggc aaaaccgccc gctgccgttt
    17221 tttgccaaaa cctcgcttgc cgccgccgaa gcgtttgccc aaaaacaaga ttgggaagcc
    17281 gccctgaaaa aagcccaaac cgcctattac ggcagcaaag tcagcaaagg gcagaaagac
    17341 tataccgaag tcgccctcgt gttcggcaac gcaagccaaa acccgctcga acagcccctg
    17401 tttgaaaacc tcgtccgtct gcttgccgac acgtttgccg cagcggaaaa aaaggaagag
    17461 gccggagcag cctgacggga tggaaaggca aatgccgtct gaaaacagca aaaaggtttt
    17521 cagacggcat tttgtttatc gtgtttaagg tgcgggatag aatgtgggcg cgccgggacc
    17581 gacgggcagg cccaaaacaa atacccaaat gcagaataag gcaatccacg caatcaagaa
    17641 gaaagcggaa tacggcaaca tcatagaaat cagcgtaccc acgcccgcat cttttttgta
    17701 tttgatcacc gtcgccataa tcagcccgaa ataactcatc atcggcgtaa taatattggt
    17761 aacggaatca ccgatgcggt aagcggcttg aatgacttcg ggcgcgtagc cggccaacat
    17821 cagcataggg acgaaaatcg gcgcagttac cgcccattgc gcggaggcgg agcctatcat
    17881 cagattgata aaagcacaaa ttaaaataaa accgataaac aacacgctgc cgcccaagcc
    17941 gacttctttt aagaacgtcg cccctttaac ggcaatatat tgcccaatat tcgtccaatt
    18001 aaaaaatgcg acaaactgtg cggcaaaaaa gatgatgacc aaataaagcc ccagagtact
    18061 catcgattcg gccatcgcat taacgacttc ctgttcgccg cgcaaacttc gggttaccct
    18121 gccataaaca atgcccggca gcgcaaacaa caagaaaata aaaacaacaa tcgattttaa
    18181 aaacggcgaa ccggaaacca atcctgtttc aggatgacgc aaaataccgt cggcagggac
    18241 gatgctccaa gccaataggg cggataaggc aacaaacacc acgccagccc aaattaatcc
    18301 tttatattcc aaaggcgtga tttcattgga atgccgaatg tctttttctt cttgtgacaa
    18361 atctgattga taagggccca attgcggttc gacgattttt tcagtaacaa aataaccaat
    18421 caaagcaatc acaaacgtac tggctaccat aaaaaaccag ttggcttcag ggcctacgac
    18481 gtagtcggga tggatgattt gcgccgcctg ttgggtgatg cctgccaaga gcggatcgat
    18541 tgtgcctaag aacagattgg ccgaataacc gcccgaaacg ccggcgaaag ccgcagccag
    18601 accggcaagc ggatggcggc cgagggaatg aaagatgatg gcggacaaag ggattaggac
    18661 gacatagccc aattcagaag cggtattaga taaaatccct gtaaaaacaa ccataaaagt
    18721 agtgagtttg cgtggagatt ttgtgagcaa taagcgcatt aatgcggaaa tcaagcccga
    18781 tttttccgca atccccacgc ccaataaaga aaccaacacc gttcccaacg gcgcgaaacc
    18841 ggtgaaattt ttaacggtat gcgtcaggat tttgatcaaa ccgtcagcat cgagcaggct
    18901 gacaacgtga atcaaaccgt catcggcacg tcctttcgca ccaacagggc gcggatcggg
    18961 gacggatagt ccgaaatacg caccggcggc agaggcaatc agcaataaca caatgaaaat
    19021 aataaaaagc gtaaccgggt gcggcaacat attgcccagc cattcgactg tgcgtaaaaa
    19081 tcgtccgtcc cgttgcgtat cggtttgact catctgcttc tccttaaaaa atatattgag
    19141 tgcattaaat ttttgaatgt aacatgaagt gttttgtaag caaactaaaa taatcgcata
    19201 tgtatataac cgcaatagat taaaacagaa aatgcccaca tcgttattct cataaaggcg
    19261 gtaatccgaa aacttgacgt taaagcctta tcaggaatga ctggaactca acaaagcggt
    19321 ttcccgcttt cgcggaaatg gcgggatttt cggtttttgt ttgcaaaaat gccggagaaa
    19381 aatgcagttt acattttgaa tggtattgtt attaattgta aataaatatt ggaaggtaaa
    19441 tgatatgaaa aatgaaattc aaaaaattat ggacaaatat gatccatggc atgaagatga
    19501 ttttgagtcg tatgaaaaca ttgccagaga tgtatcgttg atgacagata aaacgttcat
    19561 tgaacattat ttgttagaag tttattcaga agaaaacgga cattttgacc aagagaatgt
    19621 ccatgcaatg atagaagaaa ttaaaaatgc aatttagtga atatgtattt ttagaagaag
    19681 gctttgtgtc attttgatgc cgtctgaaag ggtttgttcg tttcagacgg cattgttacc
    19741 aaggcttgat tatttccggc gcaggtcggg atggttttcc aagttgtcca tcattatccc
    19801 gatgatgcgc ggggcggttt tgcccaaatc gaaacgttcg caggaagaga accaccgcca
    19861 aatcagcccg tccaaggttg atttgatgaa gatgaccgct gtttccttgt ccaaatcgtc
    19921 agccaaatcc tgattttcca ccgcttcggt caaaacggcg gtaattttct cgcgccagat
    19981 tgcctgatgc ttgcgggcaa tggcgataac ggcggcgttt tgttccgtgt gttcgcattt
    20041 taaaaacagg atgttgtgga atttgtagta gatgtcgttg ctttgcagcc gctcgaaaaa
    20101 gtgcagcagc gtgtggcgga ataccgccca agaccctcct tcggcatctt cggcatcttg
    20161 cgcgatgcag ttttcgatgt cgtcgcagat acgttggaac agcgcgtcga acaagtcttc
    20221 cttatttttg aaatgccaat agagcgcgcc gcgcgttacg ccggcggctt gggcgatttc
    20281 gttgagcgag gtgcgcgcaa tccctttgcg gtaaaaggtt tccaaggcgg caagcatcag
    20341 gtgttctttg gttttcaagg cttcggtttt ggtttttctc ataatggcgt tttcgtttcg
    20401 ggtcggtttg atgagggcgg attataaaaa agactttgta accatgcaat cgtgtatgta
    20461 taatgaaacc catgaaattg agactacatc tcaactttga aaacaggcat aacctgcttt
    20521 gcaacccgtt cgaacattcg ggtatcaaag caggcggttt ttatatcctg aaatatagtg
    20581 gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtaca
//
LOCUS       DAXHPG010000019        19937 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXHPG010000019 DAXHPG010000000
VERSION     DAXHPG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19937
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.019"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(133..1266)
                     /gene="nrdB"
                     /locus_tag="ACFM6F_000506"
     CDS             complement(133..1266)
                     /gene="nrdB"
                     /locus_tag="ACFM6F_000506"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC6163978.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGMHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(1327..2514)
                     /locus_tag="ACFM6F_000507"
     CDS             complement(1327..2514)
                     /locus_tag="ACFM6F_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HFC6163979.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(2507..3520)
                     /gene="dcm"
                     /locus_tag="ACFM6F_000508"
     CDS             complement(2507..3520)
                     /gene="dcm"
                     /locus_tag="ACFM6F_000508"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFC6163980.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(3578..5857)
                     /gene="nrdA"
                     /locus_tag="ACFM6F_000509"
     CDS             complement(3578..5857)
                     /gene="nrdA"
                     /locus_tag="ACFM6F_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC6163981.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTSQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPCVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            6386..6701
                     /locus_tag="ACFM6F_000510"
                     /pseudo
     CDS             6386..6701
                     /locus_tag="ACFM6F_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
     gene            complement(6755..7522)
                     /locus_tag="ACFM6F_000511"
     CDS             complement(6755..7522)
                     /locus_tag="ACFM6F_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC6163982.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGALAALDIGLEVGRPTPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPICLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(7610..8437)
                     /gene="mutM"
                     /locus_tag="ACFM6F_000512"
     CDS             complement(7610..8437)
                     /gene="mutM"
                     /locus_tag="ACFM6F_000512"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC6163983.1"
                     /translation="MPELPEVETTLRGITPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDERIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPYRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(8488..9153)
                     /locus_tag="ACFM6F_000513"
     CDS             complement(8488..9153)
                     /locus_tag="ACFM6F_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFC6163984.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSVDMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVRRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            9332..11308
                     /locus_tag="ACFM6F_000514"
     CDS             9332..11308
                     /locus_tag="ACFM6F_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6163985.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDIYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIVRLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            11376..12068
                     /locus_tag="ACFM6F_000515"
     CDS             11376..12068
                     /locus_tag="ACFM6F_000515"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC6163986.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPAGETLCETLKNGVLLHD
                     ENETVYAADAVLESPSALLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKF"
     gene            12594..13909
                     /locus_tag="ACFM6F_000516"
                     /pseudo
     CDS             12594..13909
                     /locus_tag="ACFM6F_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519747.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            14153..14809
                     /gene="cmk"
                     /locus_tag="ACFM6F_000517"
     CDS             14153..14809
                     /gene="cmk"
                     /locus_tag="ACFM6F_000517"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC6163987.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            14965..16650
                     /gene="rpsA"
                     /locus_tag="ACFM6F_000518"
     CDS             14965..16650
                     /gene="rpsA"
                     /locus_tag="ACFM6F_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC6163988.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGDILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            16661..16975
                     /locus_tag="ACFM6F_000519"
     CDS             16661..16975
                     /locus_tag="ACFM6F_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC6163989.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(17326..17733)
                     /locus_tag="ACFM6F_000520"
     CDS             complement(17326..17733)
                     /locus_tag="ACFM6F_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC6163990.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            17881..18990
                     /locus_tag="ACFM6F_000521"
     CDS             17881..18990
                     /locus_tag="ACFM6F_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HFC6163991.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPAKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGTAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFDLMHEGKSIRAVIHY"
     gene            18999..19826
                     /gene="fghA"
                     /locus_tag="ACFM6F_000522"
     CDS             18999..19826
                     /gene="fghA"
                     /locus_tag="ACFM6F_000522"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC6163992.1"
                     /translation="MKLIEQHQIFGGLQQVWAHYAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDAAYDLGQGAGF
                     YLNATEQPWVTNYQMYDYILNELPSLIEEKFPTNGKRSIMGHSMGGHGALVLALRNRE
                     RYQSVSAFSPILSPSLVPWGEKAFSAYLGKNREKWQQYDADSLIRQGCKVQGMRIDQG
                     LEDEFLPTQLRTQDFIETCRAANQPADVRFHKGYDHSYYFIAGFIGEHIAYHAAFLK"
BASE COUNT         5273 a         5164 c         4461 g         5039 t
ORIGIN      
        1 gatttttgtt aatccactat aatccactat aaaaggaaca ataaccgatc ctacccgctg
       61 tttttcccat catacaacat acaaatgccg tctgaaacat ccggcttcag acggcatttt
      121 ttcaaaaaac atttacaact caaaatcgcc caaatcatcc gtattcactt cagaatcgat
      181 ctgaccgatc aaataagagg atatttccac ttcctgcggc gcgacctgta cgttgtcgga
      241 cgacagccac gcattaatcc acggaatcgg gttttgattt gcgccttcaa atccggccgg
      301 caaccccacc gcctgcatac gcagattggt aatatattcg acgtattggg ataagatttc
      361 tttgttcaaa ccaatcatcg aaccgtcttt aaacaaatat gccgcccatt ctttttcctg
      421 ttccgccgct tttttgaaga gttggaaaca ttcgtcctgc aactcggcag caatttctgc
      481 catttcagaa tcatcaacac ccgaacgcat cagattaagc atatgctgcg tgccggtcag
      541 gtgcagggct tcgtcgcggg caatcagttt gatgattttg gcgttgcctt ccatcaactc
      601 gcgctcggca aaagcaaacg agcaggcgaa tgaaacgtag aaacggatgg cttccaacac
      661 gttgacgcac atcaggcaga gatagagttt tttcttcaac ccgcgcaaag acacagtaat
      721 gggttttccg ccgacattat gcatcccttc gcccaacagg ttgtaatact gggtgtattc
      781 gattaagtca tcgtaatagc aggcaatgtc ttcggcgcgg gcggtaatgt attcgttttc
      841 gacaatatca tcaaacacga ccgacggatc attcacaata ttgcggatga tgtgggtata
      901 gctgcgcgag tgtatggttt cgctgaagct ccacgtttca atccacgttt ccaactcggg
      961 aatcgaaacc aaaggcagca aggcaacatt cggactgcgc ccttggatgg aatcgagcag
     1021 tgtttgatat ttcagattgc tgatgaaaat atgtttttcg tgttcaggca ggttggcgta
     1081 gtcgatacgg tcgcgcgaca cgtcgatttc ttccggccgc caaaaaaacg acaattgttt
     1141 ttcaatcagt ttttcaaata cctcgtattt ctgctggtca taacgggcga cattaaccgg
     1201 ctgaccgaaa aacatcggct cattcagcgc gtcgtttttg gttttgggaa aggtgctgta
     1261 tgacatagtt aagtgttcgc aggacataat ctattctcta tattaattaa aaatgatctg
     1321 taagttttaa ctaacctgct gagactttat atatttaata aaattttcta aagttaaaac
     1381 atgggttttc ccaaaagttc caaatgtttt tacggattct ttagggacaa ctaaggttaa
     1441 attctcatca ctcatttcct gaatttgagt atcagatata ccttcttgta gagtaaataa
     1501 atgcttatga ggaattcgat ctgcttcatt taaaacttgg cgccagcgat ccttacaagt
     1561 agtctttgaa cctagcattg tcagcttttc agtttgaaat atgaaattcc ctgattcatc
     1621 catcgcatgg tactcttcac ttccaggaaa taaaaaatct ggtttctttt tcccttctgt
     1681 cttcgcttgc gtctcaaatt ttaaggaaaa ttcgtcaaat attcttgata aatgtaactc
     1741 taaagatttt cctgctcttg cttttctgcg atttgtaaaa gaatgtgcaa aattaatgaa
     1801 gctatccaaa tcagtaattt ttgatttaat aatttcaaat tcacgttttt caaaaatgga
     1861 aaataattca tattcctttg aaacccattg ttttaacaca ttactaacgt tagttttatt
     1921 taatgcaata ttttcccttg ccaaaactgc catttcttca gttttgggga agttaggaaa
     1981 tagtgaaaat aattttgtta ggttatcctc tacttcttgt tttttaggta aatataaact
     2041 tcctggtaaa atgttagttt ctgcgataaa tatttcaata tcttcatctg aagataaaac
     2101 aaaagcatgg aataataaat ctttataaca agctctgcac aataccaata aggaaccact
     2161 atatttatca cttaaaaatt caaaattctt gccaaaaccc gtaattctcg cctcatttct
     2221 cgtgccttgg ccgtaatatt taaaattgct attagtgacg cttccatctt gccaattaat
     2281 tttgattgaa atagttttat ttgttccctt ttggcaaact acatcaaaga ggtcgtctga
     2341 aaaatgtttt tgaatataaa atcctgattg gtgactacca gtagtaccaa catcattggg
     2401 acgaatataa cgacagtata ctgcaattga tttatttgct gtctgtattg ctgaagctac
     2461 taagtcatcg gatttattta ctacttgtat tgtctgagct gccaagtcat tcatttttta
     2521 actcatttat aatttgttta gcaatagctt gaaccaacgg tacagcaatt gaattgccaa
     2581 actgcttgta tgcagctgtc ttggatactg catcaataac aaaatcttta ggaaatccca
     2641 ttaaacgcga gcactcccta ggtgtcagct tcctaggatt ttttcctttc tgagggatga
     2701 gtatttcgga accatctttg taatatcgtg cagatagagt tcgtgatatt ccatctaaat
     2761 caactaatcc aaaaccaaat ccattaccct ttgccttatg ttttttagcg taattttgaa
     2821 ggtaaagcca taagttatca gaaagagtaa aagaattatc tacatcatct tccaaaattt
     2881 gctttaattt tggttgtgat tctggtgggg aaggaaaatt gaaatttatt tccttattaa
     2941 aatattgtct atcaaaacct acaataaaaa tacgctccct attttgagga acataatatt
     3001 ttgcattcat aacttgataa aatatctgat agtcaagctc ttctaaagtc cctttaatta
     3061 ctttaaatgt atttcctttg tcatgcgaaa caaggttttt cacattctct aaaagaaaaa
     3121 ttttaggtcg atgttttcca ataatttcag caacatcaaa aaatagagtt ccctgcgcct
     3181 tatctaagaa gcctgtttct cgtcctaggc tttttttctt tgaaacacca gctatagaga
     3241 atggctgaca cgggaatcct gctgttaata catcaaactt acttggaata gctgctttgg
     3301 tttcctttaa tgtaatatct ccataaggaa tatcattaaa atttacttgg taggtttgac
     3361 gggctttatc atcccattca ctagaaaata cacatcgccc accaacattc tccattgcaa
     3421 tgcgaaaacc acctattccc gcaaataaat caataaaagt aaatttctca ttatttaagg
     3481 taactatatt atctaattga ttttgtattt tatttttcat attttatatt tcagatgatt
     3541 tattaatcta aaggccatct gaaaactccc cctttcatca aatcttacaa gccccgcccg
     3601 cgcagccgtc atcctgaata tcggtctgcg tatcgtccgc gccgtcgcgg gtgttatggt
     3661 agtacagggt tttgacgccg tatttgtagg cggtcagcag gtctttgagc atttgtttca
     3721 tagaaacttt gccgccttcg aatttgcccg ggtcgtaggc ggtattggcg gaaatcgatt
     3781 gatcgacgaa tttttgcatc acgccgacaa gtttcaggta gccttcgttg ccgggaagct
     3841 gccacagggt ttcataggca tttttcaggg tttcaaactc cggcacgact tgtttcaaaa
     3901 tgccgtcttt cgatgctttg accgttacca atccgcgcgg cggctcgatg ccgttggtgg
     3961 cgttggcgat ttgcgaactg gtttcagacg gcatgagcgc ggtcagggtg gagttacgca
     4021 ggccgtattt gacgatgtcg gcacgcaggc tttcccagtc gtaatgcaaa ggctcgccgc
     4081 agacggcatc caaatctttt ttgtaagtgt cgatgggcag tttgccttgc gaataaacgg
     4141 tttggttaaa gagcgtgcac gcaccgtatt ctttggcaag gtttgccgat gctttgagca
     4201 ggtaatactg tatggcttca aaggtacggt gggtcagacc gagcgcggag ccgtcgctgt
     4261 agcggacacc gtttttcgcc agataataag catagttaat cacgccgatg ccgagcgaac
     4321 ggcgacccat agtagaggta cgcgcggctt ctaccggata tccctgataa tctaaaagtg
     4381 catcgagcgc acgcacggtc aagtcggcaa gcccttccaa ttcgtccaag ctgtttaatg
     4441 cgcccaagtt aaaggcagac agtgtacaca gggcgatttc gccgttcgga tcgttgatat
     4501 tgtccagcgg tttggtcggc agggcgattt ccatacacaa gttggactga tgaacaggcg
     4561 cgacgcacgg atcgaacggg ctgtgcgtat tgcagtgatc gacgttttga atgtagatgc
     4621 gcccggtccc ggcacgctcc tgcatcagcg tggaaaacag gtcggcagcc ggaatgatac
     4681 gcttgcgtat gttcgggtct tgctcgtatt tcgtatagag ccgctcaaat tcgtcttggt
     4741 cggcaaaaaa cgcttcgtac aaccccggaa cctcgttggg cgaaaacagc gtaatgttgc
     4801 cgcccttaat caggcgggtg tacagcaggc ggttgatttg cacgccgtaa tcaagctgac
     4861 ggatacggtt gtcttccaca ccgcggttgt ttttcaacac cagcaggctt tcggcttcga
     4921 tatgccacaa ggggtagaac aaagttgccg ctccgccgcg cacgccgcct tgcgaacagg
     4981 atttgaccgc cgcctgaaac attttgaaaa acggaatgca gccggtatgc cgcgcttcgc
     5041 cgccccggat ttcgctgtcc aaaccgcgga tacgtccggc attgatgccg atgcccgcac
     5101 gctgggaaac gtatttcaca atcgcgctgg tggtggcgtt gatggaatcc aggctgtcgt
     5161 cgcattcgat cagtacgcag cttgagaact ggcgcgtagg cgtacgcacg ccgctcataa
     5221 tcggggtcgg cagcgatact ttaaatgtgg aaacggcatc gtaaaaccgt ttgacgtaat
     5281 cgaggcgtgt ttctttggga tatttgctga aaagacacat cgccaccaga acatataaga
     5341 actgcggtgt ttcataaatc tggctggtaa cgcggttctg taccagatat ttgccttcga
     5401 gctgtttgac agcggcatag gaaaaggaca tatcgcgttc gtggtcgata taggcgttca
     5461 gttcatcaaa ttcttcgcgg ctgtaatcct caaggatatg cctgtcgtat tttccggcat
     5521 cggtgagctt tttaacgtgg tcgtaaaggt gcggcggctc gtattcgccg taggctattt
     5581 tacgcagatg gaaaatcgcc aaacgcgcgg caaggtattg gtagtccgga gtatcttccg
     5641 aaattaaatc ggcagcggcc ttgatgatgg tttcgtggat gtcgtcggtg cggatgccgt
     5701 tgtagaactg gatgtgcgat ttcaactcga cctgcgacac ggaaacattt tccaatcctt
     5761 ccgccgccca agtaacgaca cggtgaatct tatccaaatc aatggcttct aatcttccgt
     5821 ctcgtttggt tactttcaaa tcagtcggtg tattcatcgc ttcctcttcc actcttgata
     5881 ttcaagacac ggtcttttca aataaattaa gacagacaat atagtggatt tttggcattt
     5941 tctccagtct tgacaacggt tgcatttttc agtttcaggc aatgcgcgat aaaacccgcc
     6001 tgtcgtattt ttcctataat atttgtttta tctgataatt ctttaccgat aaaaaacggg
     6061 taaatttttt gccttttgac cgactccggc tacaaggcgg tgaaataagg attttccgac
     6121 gaaaaaggaa aacttccggt tttctgcccc tgcaaattgt taaattttgc aaagtatgat
     6181 tttgccgagc cgccgccgac aaattccatt ttcttaccga ttggaattta ttattgagat
     6241 taatgtgtta tttgaatccg catatcaaac ggcaagtttt gtcggctgaa tgagtctgaa
     6301 actgccgaca atttgcccgt tccatccttc catcctatac tggaaagaat gacaaactga
     6361 aaggatcgtc atatctgtca aaaagatgct tgccatactg ttgtctgcaa tattgggact
     6421 ggtatcgaca actgctgctg ccggtacgtc agaacccgcc caccgccata ccaaacatat
     6481 ccgcaaggca aacaagcaga tgctccaccc cgaatgcagg aaatatttgg aacgccgtgc
     6541 cgcgtggtac cgatcgcaag gcaacgtgca ggaattgcgc gaaaacaaaa aggcgcgcaa
     6601 agcattccgc tccctgcctt atttataagg aacagaaaac ccaatgccgg gcggcttatg
     6661 aggctttcga tgatttcgac ggcggcaggt tcagccgtta atccactata catccgcaaa
     6721 agcgaatcat actgccacat tccgacaacc ggtttcagac agcgatatcc gcatcgtcgg
     6781 cgacaacggc acggatgctt tcttcgattt tatcttttaa agcataacgg tcttcgcttt
     6841 ccgccgcatc cgccacgcaa acgaaatcaa cttttatcgt caattttttc atagacacaa
     6901 tgcgccacag gcagatcggc aaaccgacat cggcatacga cggacgagcc gtcctttttc
     6961 ccgtttcgtc ataataacgc agcgcgaccg ccaaaacctt tgcccccgca tcgatggcgg
     7021 attggaacag cgcggctttg aacggcagga gccccagtcc ggaggaagta cgcgcttcgg
     7081 ggaaaaaact gacgttttga ccgcgttgca aggtttcgca gacggcgcgg ttaatcggtt
     7141 cgatgtcgcg ccgcgaattg cggttgatga acaccgttcc cgcgttctgc cccatcttgc
     7201 ccaataccgg ccagcttttg atttcctgct tggcgataaa gctgctcgga taaaccgcgc
     7261 tcatcgcgaa aatatccagc caggacacgt ggttggcggc aaccaagaca ccgttcggat
     7321 gttcgggtgt gggtctgccc acctccaatc cgatatccaa agccgccaaa gcccccctac
     7381 ccaactcgat taccgcccga ttacgcgact cggggcagcc gccgtcaata ccgcgcaggt
     7441 ttttgccggt tttgaacagc cagaccgtca agcggcacaa gcggcgcaga cgtgtaaaaa
     7501 atgaagcttt atttgaagac atgagcaatt ctttcccgat aatcggtgtt tgttgttgaa
     7561 acgttcggac ggcataccga taaaatgccg tctgaaaccg ttttcagccc tatttctgac
     7621 agttcgggca ataaaacgtg ccgcgctgcc ccaaagtttc tttcacaacc aaaccgccgc
     7681 actgggggca cggctgattg tgccgcccgt acactgtata ttcctgttgg aagtagccgc
     7741 ttttgccgtc gctgtccaca aaatccctca gcgtactgcc gcccgtttca atggcgcgct
     7801 gcaacaccgc tttgacggtt tcaaccaaaa gcgcgcactc tttctttttc aggcggttgg
     7861 caggacggta gggcgaaatg cccgctctga acaggctctc gttggcataa atgttgccca
     7921 caccgaccac gaccgcattg tccatcaggg caagtttgac cgcgcgcttc tgcgccttca
     7981 gccttgcata cagataatcc gcacaaaatg cctcagacaa aggctccggc cccagttttt
     8041 ccaacagcgg atgatgttct tcgattccct cataccaaag tatcgcgccg aactttcgcg
     8101 gatcgcggta acgcatgacc gtgccgtctg aaaacacaat atcgacgtga tcgtgtctgt
     8161 ccggcctgcc gatacgttcg tccgacggcg taaaaatccg caagctgccc gacatcccca
     8221 agtgaatcag caaaatcccc gtttggaaac ggacaatcag atatttcgcc cgcctgccgc
     8281 aggacaaaac ctgccggccg gacaaaatct cccccaaatc gggattaatc tgccagcgca
     8341 gcttcagttg gcgcaatacc acggcttcca ccgtttttcc ttcaatatgc ggcgtgatgc
     8401 cgcgcaacgt cgtttccact tccggcaatt ccggcataat ccctcccgac actttctccg
     8461 gcaaatgccg tctgaaacaa aacccgccta atccgcaacc cttgccgcac ccgccgcaag
     8521 ggctttgccg cccaaatacc cgtcccacgc gggcaggggc gttacgcccg ctgcccttct
     8581 gaccaaaacc aaaccgtaca ctgcggcaca ctgcggccac caacggtcgc ccgccttttc
     8641 cataaaccgc caaaagcgta tttgcccgag cgacgaaacc ggcggcagat acaccataaa
     8701 tttcccaaat tcaatatcga aaccgacatc cgcaagccgt cttttcaact cgggcagcgg
     8761 cagacaaaac cgtttttccg gcaggcgttc gccgtcaaac caacggctga atccccagag
     8821 cgaatacgga ttgaaacccg tcagcatcaa gcgtccgcac ggtttcaata tccggtgcgc
     8881 ctcggacagg atttgcgaag gaacaccgcc ttcaagcgta tgcggaaaaa gcagcatatc
     8941 cacagaaaca tccgccaaag ccatattttc cgccgacatc gacatatctc gcggcacaca
     9001 gacaacatct tcagacaggc tcagccacgg accgcccacc tgaaccgcac acattcccga
     9061 aaaacggtat gaatccagat accgcccgaa gaaatcctgt tccaattttg caacataccg
     9121 ccccatcgcc gtatcttcaa accatgcatc catattgcgt ccgtttcaaa cagattgcct
     9181 gccgatattc tattccaaac aggatttctg tcaaaaaaca catcggccgc ccatttccga
     9241 atccgcataa agttcctgta aaacttgacg ctttttcagt caaacagtac catcggacga
     9301 taaaatatgt ttattccgcc gaatataaat catgtccaaa ctcaaaacca tcgctctgac
     9361 cgcatcaggt ctgtccgttt gtccgggttt cctatacgcc caaaacacct catcacacca
     9421 aatcggtttg gcgattatgc gcttaaactc ttcaatactc gacctgcccc cgacaaaaca
     9481 atatttccaa tccggcagcc tgtggagcga gctgcgccaa ggcttccgga tgggcgaagt
     9541 caatcccgaa ctggtacgcc gccacgaaag caaattcatc gcaagccaca gctatttcaa
     9601 cagggtcatc aaccggagta gaccctatat gtaccatatc gccaacgaag tcaaaaaacg
     9661 caatatgccc gccgaagccg ccctgcttcc cttcatcgaa agcgcgttcg tcaccaaagc
     9721 caaatcacac gtcggcgcat cgggcctgtg gcagttcatg cccgctaccg gcaggcatta
     9781 cggcctggaa aaaacaccgg tttacgacgg caggcacgac atttacgccg ccaccgatgc
     9841 cgcactcaac tatctgcaat acctctatgg actgttcggc gactggccgc tcgcctttgc
     9901 cgcctacaac tggggtgaag gcaacgtcgg acgcgccatc aaccgcgccc gcgcccaagg
     9961 gctcgaaccg acctacgaaa acctgcgtat gcccaacgaa acgcgcaact atgtccccaa
    10021 gctgctcgcc gtgcgcaaca ttattgccac tccccaatct ttcggtatga atatcagcga
    10081 catagacaac aaaccctatt ttcaggcagt cgaaccggat cgtccgctcg acaacgaagc
    10141 catcgtccgg cttgccggca tcacgcaaag cgagctgctc gccctaaacc ccgcattcaa
    10201 cgtccccgcg tttatcccca aaagcaaacg caaactgctg cttcctgtcg catccgtcca
    10261 aaccttccaa agcaactacc tcaacgccgc acccgacagc ctgttttcat gggaagtcta
    10321 tacgcctgcc gccaaaacca gcctgtccga catctcgacg gcaaccggca tgagcattgc
    10381 cgacatcaaa cgcctcaaca acctgaacgg caaccttgtc aacgcaggac gcagcatcct
    10441 tgtcgccaag aacggcaaaa cccttcagac ggcatcggaa tccgtcgttt ccatcgacat
    10501 cgacaatacg cccgacacct accgttccaa tatgccggca ggcacggtga acgtcggcat
    10561 tgcccgaatc cgacccgccg ccgcacagac agcggacatt accgtcgcac ctttgccgca
    10621 gaaaaccgtc cgtacggaac ccgatcccct tgtccgtatt gccgaacctg cccttgcgac
    10681 agccgcagcg caacctcaaa ccgaaaaaca gaccgccatg ccgtctgaaa cccaaaccgc
    10741 aacactcgcg cagatcatcc cccaaaacga catgcaggcg gcagacgaac tcatgcagct
    10801 tgttgcccga aacaacctgc gccgacaggc tgaagaaacc atctccgccg tcatcggcac
    10861 gcctgacaca gttgccgaac acaacatttc ctcatctccg caacataccg ttgccgccga
    10921 cggcaaacgc cgggcacgtt tggaaacgcg cgtagccaaa gctgccgacg gcgaagccga
    10981 aatctccccg ctccatgcca gcatccaccg cgttgtagaa ggcgacaccc tgttcaacat
    11041 tgccaaacgc tacaacgtca gcgtagccga cctgattgtc gccaacaaca tcaaaggcaa
    11101 caccatccaa aaaggacagg tactccgcct ggcgcaggca gcccctgccc aaacccgtat
    11161 tgaaaaagta tcctacaccg cgcgcaaagg cgacaccttc aaaagtatcg ccgcgcgctt
    11221 caatatccat atcgacgaca tccgccggct caatcccaac ctgaacacca tcaatccggg
    11281 acagagggtc aaactgattg gaagctgatt cggatacggc acatgacagg actttctcag
    11341 tcctgtcttt ttcatcccac atttcccata ccatcatgaa acttatcaaa tacctgcaat
    11401 atcaaggcat aggaagccgc aagcagtgcc aatggctgat tgccggcggt tatgttttca
    11461 tcaacggaac ctgcatggac gacaccgatg cagacatcga ttcctcatcc gtcgaaacgt
    11521 tggatattga cggggaagca gtaaccgtcg ttcccgaacc ctatttctac atcatgctca
    11581 acaagcctga agattacgaa acttcgcaca aacccaagca ctaccgcagc gtattcagcc
    11641 tgttccccga caatatgcgg aacatcgata tgcaggcggt cggcaggctg gatgcagata
    11701 cgaccggcgt attgctgatt accaacgacg gcaaactgaa ccacaacctg acttcgccga
    11761 gcagaaaaat tcccaagcta tacgaagtta cactcaagca ccctgccggc gaaacgcttt
    11821 gcgaaaccct gaaaaacggt gtcctgctcc acgacgaaaa cgaaacggtt tacgccgccg
    11881 atgccgtatt ggaaagcccg tccgcattgc tcctgaccat taccgaggga aaataccacc
    11941 aagtcaaacg tatggtcgcc gccgcaggca accgcgtgca acaccttcat cgccggcgat
    12001 tcgcacattt ggaaacagaa aacctgaaac ccggcgaatg gaaatttatc gattgtccga
    12061 aattctaaaa taacatccaa gaattccatt tatattcatc cacatactca ttaaatatat
    12121 ggtttaaccc aatttaacgc aaaaaatcaa tttacgatat aatccatttg tctgagtaac
    12181 gcctgttcaa gcaggcttat gagtaagacg ttttccccgt aatgtgtttt gccatctata
    12241 cttctcccct tgtatagatg gttttttatt tcaaattccc tctctttcat cattattaaa
    12301 atgctatcat taatgtgatt aacaaaattt catttccata caccgatgta gaagaataac
    12361 aaattaatct atttataaac tccgattctt cctcatcggg ttatactcat cagaaatact
    12421 cactgaaata tatgcctttt acatggaaaa tcactagaca gttgaaaaac aatcagtcaa
    12481 ttcctgttaa aaaacgcgtg tagaatttcc gctttatctc aatcggacac ggtttcaata
    12541 tgtcttcccc ttcaaatacc aatcgtcaaa cctgatccag ccgattaacc tatatcctga
    12601 ctgttgccgg cgcgactgtc ggcttcggcg cgacgtggcg tttcccgtat ttggtcggtg
    12661 aaaacggcgg cggcgcgtat gtgtttttat tctgtatcgc catgctcgtt atcggcatcc
    12721 cgatgatttt ggtggaaaac gtcatcggac ggcgcaaagg cgtgaacgcg ctggatgcgt
    12781 tcggcggtcc gatgaacggc aaacccattg ctaaaatttg gaaactggtc ggctggatgg
    12841 ggctgctcgg cgcgttcggc atcatggctt attacatggc actcggcggc tgggtaatca
    12901 gctatatcgt taatattatt ggaggaaatt tgaatatttc cagccccatc gacggtgtgg
    12961 ttacaaaagg cttctttgcc gaacacattg aaacagccct tgggaaattg cgttttatac
    13021 gctgcttttt gtcgccgtga accaatggat tttggtcaaa ggcgttatcg gcggcattga
    13081 aaaagcggca aaatagctga tgccgctgct gtttttgttc ctaatcgcga tggtcgtccg
    13141 caacgttacc cttccgggcg caatggaagg ggttgctttc tatctgaaac ctgatttcag
    13201 caagattacc gccgaactgt tcgtcttcgt tttggggcag gtattttttg ccctgagcct
    13261 gggtttcggc gtgatgatta ccttgtccag ctatttggat aaaaacgaaa atctggttca
    13321 gacggcagtt atcacggcaa ttaccaatac catcatcgcc atacttgcgg gctttatgat
    13381 tttcccgtcg ctcttcagct tcggcgttgc ccccgattcc ggcccgactt tggtattcca
    13441 aagtttgccg attgtgttct cgcatatgtg ggcaggatct gtgttcgccg tcattttctt
    13501 ctcgctgctc ctgattgccg cgttgacaac ttcgctgacc atttatgaag tgttgattac
    13561 gaccattcag gaaaaaacca aaatccgccg taccgccgcg attacgattg tattggctgc
    13621 catcttcatt ttcggcaaca tcccgtccat tctgagctat ggtccgtgga aagacgtttc
    13681 cgtgttcggc aaaaatattt tcgatgcctt cgactacatc agcggcaaca tcttgtttat
    13741 gctgaccgcg ctcggttccg cgctgtttgc cggttttgtg atgaaggacg aagcgaagga
    13801 cgaattgctt tataaaggca accatacgac ggtcaatatt tggtttgctt atgtgaaata
    13861 tcttgtgccg ctggtgattc tgctgatttt cgtcagcaac ctgttctaat ctgcagcaat
    13921 cgatgccgtc tgaaggtcat acctctttca gacagcattg ccttgatgcc cgccacaata
    13981 cccgcaacgc cggaatccga accggcttgc cggcttctgt cggatgtttt cgggcaggcg
    14041 gcgttccgtc tgcttagaca atcgtccttt aaaacaggta gaatccgccc caacgggaaa
    14101 cacatccttc agacggcaaa acccataccc caaaccatca ggaatccccc ttatgaacag
    14161 acaaaaagtc atcgccatcg acggcccggg cgcatcgggc aaaggcacgg ttgccgcccg
    14221 cgttgccgcc gcattgggat acgattatct cgataccggc gcactctacc gcctgaccgc
    14281 cctatatgca caaaaacaag gcgtgggatg gcacgatgaa gaaaacgttt ccgaactggc
    14341 aaaaaaactg cccgccgtat tttcaggcag ccgcatcctg ctcggcggcg aagacgtttc
    14401 agacggcatc cggacagaag ccatcggcat gggcgcatcc gcagtcgcac agttgcctaa
    14461 agtccgcgcc gccctgctgc aacgccaacg cgattttctg accgaaaaag gactggtcgc
    14521 cgacggacgg gacaccggat cggtcgtctt cccccaagcc gaacttaaaa tcttcctgac
    14581 ggcagaatcc aaaatccgtg ccgaacgccg cgccaaacaa atcggcatcc cctgcgaagg
    14641 tttggcattc gagcgcatcc tgtccgacat cgaagccaga gacgaggcag accgaaaccg
    14701 caaggttgcc cccctgaaac aacaacccga tgccctgctt ttggatacga gccgcctgac
    14761 tatagaagaa actgtaaaaa aagtgcttga ttggtatcgt aaagtttaaa ttttcaggta
    14821 taatcgcccg cattatgttt cagacggcat aaaaatcccc catatgccgt ctgaaacctt
    14881 atttacccgt ctgccctgcc aaggacggca gacacccacc aacccacccc gcaccccttg
    14941 gcggtgtacc gaaaagagtt atatatgtct atggaaaatt ttgctcagct gttggaagaa
    15001 agctttaccc tgcaagaaat gaacccgggt gaggtgatta ccgctgaagt agtggcaatc
    15061 gaccaaaact tcgttaccgt aaacgcaggt ctgaaatcag aatccctgat tgatgtagct
    15121 gaattcaaaa acgctcaagg cgaaattgaa gttaaagtcg gcgacttcgt taccgttacc
    15181 atcgaatccg tcgaaaacgg cttcggcgaa accaaactgt cccgcgaaaa agccaaacgc
    15241 gctgccgatt ggattgccct ggaagaagcc atggaaaacg gcgacatcct gtccggcatc
    15301 atcaacggaa aagtcaaagg cggcctgacc gttatgatta gcagcatccg cgcattcctg
    15361 ccgggttctt tggtcgacgt acgtcctgta aaagacactt ctcacttcga aggcaaagag
    15421 atcgaattca aagtgatcaa actggacaaa aaacgcaaca acgtcgttgt ttcccgccgc
    15481 gccgttctgg aagccacttt gggtgaagaa cgcaaagccc tgctggaaaa cctgcaagaa
    15541 ggctccgtca tcaaaggcat cgttaaaaac attaccgatt acggtgcatt cgttgacttg
    15601 ggcggcatcg acggtctgtt gcacatcacc gatttggcat ggcggcgcgt gaaacacccg
    15661 agtgaagtct tggaagtcgg tcaggaagtt gaagccaaag tattgaaatt cgaccaagaa
    15721 aaacaacgcg tttccttggg tatgaaacaa ctgggcgaag atccttggag cggtctgacc
    15781 cgccgttatc ctcaaggcac ccgcctgttc ggcaaagtat ccaacctgac cgactacggc
    15841 gcattcgtcg aaatcgaaca aggcatcgaa ggtttggtac acgtctccga aatggactgg
    15901 accaacaaaa acgtacaccc gagcaaagtc gtacaactgg gcgacgaagt cgaagtcatg
    15961 attttggaaa tcgacgaagg ccgccgccgt atctctttgg gtatgaaaca atgccaagcc
    16021 aatccttggg aagaatttgc cgccaaccac aacaaaggcg acaaaatctc cggcgcggtt
    16081 aaatccatta ccgatttcgg cgtattcgtc ggcctgcccg gcggcatcga cggtttggtt
    16141 cacctgtccg acctgtcctg gaccgaatcc ggcgaagaag ccgtacgcaa atacaaaaaa
    16201 ggcgaagaag tcgaagccgt cgtattggca atcgacgtgg aaaaagaacg catctccttg
    16261 ggtatcaaac aactggaagg cgatccgttc ggcaacttca tcagcgtgaa cgacaaaggt
    16321 tctttggtta aaggttccgt gaaatctgtt gacgccaaag gtgctgttat cgccctgtct
    16381 gacgaagtag aaggctacct gcctgcttcc gaatttgcag ccgaccgcgt tgaagatttg
    16441 accaccaaac tgaaagaagg cgacgaagtt gaagccgtca tcgttaccgt tgaccgcaaa
    16501 aaccgcagca tcaaactttc cgttaaagcc aaagatgcca aagaaagccg cgaagcactg
    16561 aactccgtca atgccgccgc caatgcgaat gccggcacca ccagcttggg cgacctgctg
    16621 aaagccaaac tctccggcga acaagaataa ggttgcagac atgacaaagt ctgagttaat
    16681 ggttcgtttg gcagaagtgt ttgccgccaa aaacggcacg catcttctgg caaaagacgt
    16741 agagtacagc gtaaaagtct tggttgacac catgactaga tcgcttgccc gaggtcaacg
    16801 catcgaaatc cgcggtttcg gcagcttcga tttgaaccat cgtcctgccc gcatcggtcg
    16861 caatcccaaa accggcgagc gtgtggaagt acctgaaaaa catgtacccc acttcaagcc
    16921 cggtaaagaa ttgcgcgagc gggtcgactt ggctttaaaa gaaaatgcca attaaacctt
    16981 agcatcaaaa cgccgctgtt acgcggcgtt ttttctgtgg tttaacttca tccgctgctt
    17041 caataccttg agccaagcaa gcaacggatt agagcgtgga tttttttata gtggattaac
    17101 aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga acaattctct aaggtgctga
    17161 agcaccaagt gaatcggttc cgtactattt acactgtctg cggcttcgtc gccttgtcct
    17221 gatttttgtt aatccactat atcattgctt acaatccgct ttttaaacaa caaatttttg
    17281 atttctatta cgaacaggac aaaaatcctg cttattgcac taaaactaag ccgtttcagg
    17341 aatttgcggc aaatttacgg cttttaccga acctaatgct ttcgcttttt ggtaaaacgc
    17401 caatttgtat tcaagcaaat ctaaatagcg ttttaattcg gcaatttgac acttcacatt
    17461 ttctatttga ttttcaaaca aggaaaggcg ttcttcaatg gtatcgtcgc caatgacggt
    17521 acattccgca aagcgtttga tgtcttttaa gctcattccc gtatttttca agcattgcaa
    17581 taagcccaac cattgcaaat cgttatcggt aaaacagcgg ttaccgtatt catcacgtcc
    17641 gatattaggc aacaagcctt ctttgtcgta aaaacgtagg gtgtgggcgg agatgcctat
    17701 tttttcggcg gctttggcag tggtataagt cattttccct ccttctaaac aaaaacagta
    17761 aaaaacactt gccttagagt taactctaaa gtgtaaactg ttgctatgtt gctcaggtaa
    17821 ggcaactttg tcaatgaatt aagaggaaag acaatggaaa tgaaacaaac cgattcaacc
    17881 atcaaatctc gtgcggcggt ggcattcgcc cctaaccaac ccttacaaat tgtggaaatc
    17941 gacgtagaaa tgccgcgtaa aggcgaggtg ttaatccgca atacccacac tggcgtgtgc
    18001 catactgatg cgtttacgtt atcaggaagc gatcctgaag gcgtattccc tgtggtgctt
    18061 ggacacgaag gtgcgggtgt ggtcgttgct gtgggcgaag gtgtgtcaag cgtaaaaccg
    18121 ggtgatcacg tgattccgct ttacaccgcc gaatgtggcg aatgtgagtt ttgttgttca
    18181 ggtaaaacca acttgtgcgt ctcagtgcgt gatacacaag gtaaaggctt aatgccggac
    18241 ggcacgacgc gtttttctta tcaaggtcag ccaatctatc actatatggg ctgttcgact
    18301 ttcagtgaat actccgttgt tgccgaagtt tcactggcga aaatcaaccc tgaagccaac
    18361 catgaacaag tatgtttgct cggctgcggc gttaccacag gtattggtgc ggtacataat
    18421 acggcaaaag tgcaagaagg cgactctgtt gccgtgtttg gcttaggcgc gattggtttg
    18481 gcggtggtgc aaggtgcgcg tcaagccaaa gccggccgta ttatcgccat tgataccaat
    18541 cccgcaaaat tcgagttggc aaaacagttt ggtgcaaccg attgtttgaa cccgaacgat
    18601 tacgataaac cgatcaaaga tgtgttgtta gacatcaaca aatggggcat cgaccacacc
    18661 tttgaatgta tcggcaatgt aaatgtaatg cgtcaggcat tagaaagcgc acaccgtggt
    18721 tggggacaat ccattatcat cggcgtagcg ggtgctggcc aagaaatttc cactcgtccg
    18781 ttccagttgg taaccggccg tgtttggaaa ggcactgcat ttggcggcgt gaaaggccgc
    18841 tccgagcttc cgaaaatggt ggaagattca atgaaaggcg acatcgagtt agaaccgttt
    18901 gtgacccaca caatgacgct cgatcaaatc aatgaagcct ttgatttaat gcatgaaggt
    18961 aaatcgatcc gcgctgttat tcattactaa ggtacgcgat gaaactgatt gagcaacatc
    19021 aaatttttgg cggtttgcag caagtttggg cgcattatgc ccaaacgctg cagtgcgaaa
    19081 tgaaatttgc cgtctatttg ccaaataatc cggagaatcg accgcttggt gtgatttatt
    19141 ggctttccgg cttgacttgt acggaacaaa atttcattac caaatcaggt tttcagcgtt
    19201 atgcggcaga acatcaagtg attgtggttg cccccgatac cagccctcgc ggagagcaag
    19261 tgccgaacga tgcggcttac gatttagggc agggtgcggg cttttatctg aatgcgaccg
    19321 agcagccttg ggtgacgaat tatcaaatgt atgattatat tttgaatgag ttgcctagtc
    19381 tgattgagga aaaatttcct accaacggca aacgttccat tatgggacat tcaatgggcg
    19441 gacatggcgc attggtattg gcactgcgaa accgggaacg ttatcaaagt gtttctgcct
    19501 tttcgcctat tttatcgcca agcctcgtgc cttggggaga aaaagccttt tctgcctatt
    19561 tgggaaagaa tcgcgaaaaa tggcagcaat atgatgccga ctcgctcatc cgacaaggct
    19621 gtaaggtgca aggtatgcgc attgatcagg gcttggaaga tgagtttttg ccgacacaat
    19681 tgcgtaccca agattttatc gaaacctgtc gtgcggcaaa ccaaccggcc gatgtgcgct
    19741 tccacaaagg ctatgatcac agctattact tcatcgccgg ttttattggc gagcatattg
    19801 cctatcacgc ggcatttttg aagtaagcca aagagcgttc agtgttcaaa gcagttttgg
    19861 gatagccggc acgagggcgg taagaagtgc cggcataaac gtatgccgtc tgagccggaa
    19921 gaagccgact ctacgga
//
LOCUS       DAXHPG010000020        19814 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXHPG010000020 DAXHPG010000000
VERSION     DAXHPG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19814
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.020"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(199..274)
                     /locus_tag="ACFM6F_000523"
     tRNA            complement(199..274)
                     /locus_tag="ACFM6F_000523"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(239..241),aa:Asn,seq:gtt)
     gene            complement(367..726)
                     /locus_tag="ACFM6F_000524"
     CDS             complement(367..726)
                     /locus_tag="ACFM6F_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC6163993.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(749..1393)
                     /locus_tag="ACFM6F_000525"
     CDS             complement(749..1393)
                     /locus_tag="ACFM6F_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFC6163994.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADV
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1395..1931)
                     /locus_tag="ACFM6F_000526"
     CDS             complement(1395..1931)
                     /locus_tag="ACFM6F_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HFC6163995.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1943..3040)
                     /locus_tag="ACFM6F_000527"
     CDS             complement(1943..3040)
                     /locus_tag="ACFM6F_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6163996.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3043..4479)
                     /locus_tag="ACFM6F_000528"
     CDS             complement(3043..4479)
                     /locus_tag="ACFM6F_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6163997.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4699..5481)
                     /gene="bioC"
                     /locus_tag="ACFM6F_000529"
     CDS             complement(4699..5481)
                     /gene="bioC"
                     /locus_tag="ACFM6F_000529"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC6163998.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFITHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVRLTYHPLFFIARYSAEGRQ"
     gene            complement(5469..6116)
                     /locus_tag="ACFM6F_000530"
     CDS             complement(5469..6116)
                     /locus_tag="ACFM6F_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC6163999.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDVNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPFA
                     VFKGTLENLTEDTRLKFERRICGDKASFEDYQQFPARPFDEIHQELTSLFKMIGQDRC
                     TDLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6126..7268)
                     /gene="bioF"
                     /locus_tag="ACFM6F_000531"
     CDS             complement(6126..7268)
                     /gene="bioF"
                     /locus_tag="ACFM6F_000531"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC6164000.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEAALAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            7491..8567
                     /locus_tag="ACFM6F_000532"
     CDS             7491..8567
                     /locus_tag="ACFM6F_000532"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFC6164001.1"
                     /translation="MICRKPIGHKPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8774..10240)
                     /locus_tag="ACFM6F_000533"
     CDS             complement(8774..10240)
                     /locus_tag="ACFM6F_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC6164002.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFIGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10438..11361)
                     /gene="speB"
                     /locus_tag="ACFM6F_000534"
     CDS             complement(10438..11361)
                     /gene="speB"
                     /locus_tag="ACFM6F_000534"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC6164003.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11460..13352)
                     /gene="speA"
                     /locus_tag="ACFM6F_000535"
     CDS             complement(11460..13352)
                     /gene="speA"
                     /locus_tag="ACFM6F_000535"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC6164004.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13519..13803)
                     /locus_tag="ACFM6F_000536"
     CDS             complement(13519..13803)
                     /locus_tag="ACFM6F_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6164005.1"
                     /translation="MKKLLLVAVVSLSAAAAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13954..15762)
                     /gene="aspS"
                     /locus_tag="ACFM6F_000537"
     CDS             complement(13954..15762)
                     /gene="aspS"
                     /locus_tag="ACFM6F_000537"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC6164006.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGTKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(15820..16521)
                     /locus_tag="ACFM6F_000538"
     CDS             complement(15820..16521)
                     /locus_tag="ACFM6F_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC6164007.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16664..17812)
                     /locus_tag="ACFM6F_000539"
     CDS             complement(16664..17812)
                     /locus_tag="ACFM6F_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC6164008.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17972..18235)
                     /gene="rpsT"
                     /locus_tag="ACFM6F_000540"
     CDS             complement(17972..18235)
                     /gene="rpsT"
                     /locus_tag="ACFM6F_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC6164009.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18538..19680
                     /locus_tag="ACFM6F_000541"
     CDS             18538..19680
                     /locus_tag="ACFM6F_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC6164010.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDVCLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5102 a         5115 c         4613 g         4984 t
ORIGIN      
        1 gccgtactgg tttttgttaa tccactataa aatcgagttt atcctagctg tccaggacag
       61 cccctatttt ttcataacca tcacgaaagg aattttgcaa agattttatt ccatctcaaa
      121 acaatcatct caaaaatgcg tttctgaccg ccggtaaaac aaaaccctct aagaaaatac
      181 ttagagggtt ttgaaatttg gctccccgac ctgggctcga accagggacc tgcggattaa
      241 cagtccgtcg ctctaccgac tgagctatcg gggaataagc gcgaataata aaacagaaat
      301 accgaacgtg tcaatataaa ttgacaatcg attgcacttt atctgcaagc ccgcaatctt
      361 tatatgttat atggcttccg caatatgcct tgccgcacgt ttggcatcca ataggattaa
      421 accaagtctg ccggtttctt ttgccaccag caccaacacg gcatctttac ccgcctgact
      481 taaaaggata tagcctgatt ttcctttaat catcacttgt tccaattccc cgcaggcgag
      541 ttcctgcacc gagcgactcc ccaaagcaag caaagtggca gaaatcgccc ctaccctgtc
      601 cgaattcaaa tgtgaaggaa gcattgtcgc catcggcaat ccgtcggttg agataacggc
      661 agacgcgata atatccgtag atgtattgtt taaatcttca aggattgaaa tcaataattg
      721 ctgcataacc cctcctctct cccaagtttt acacgcggtt gctgtaacgg cggtataaaa
      781 tccttaccaa agtaacaaat gcctctttgc ccaaatcggg aatgccgccg ataaccaaaa
      841 taaatttggt tgaaccgata tacaatggga aaaatgtcaa ttcgctctga ccggaaggat
      901 cgcaaacgcc ccaagcgtta ttgttgatat acaggttgtt cttaatcagc agccggtatt
      961 tcttttccat ctgtgcgact tctgccgcca acaaccccaa ctcttccgcc gcctcatgat
     1021 ggaaattggc gttggcaaga tacagaccgt tccgatcgac caataacgcc ttaccgctgc
     1081 cggacaattg ttccatcagc aacggcaatt gctcgtccga caaattgatg ccgtctgaat
     1141 gaccgttttc atcgccatag aggaattcga gtttttgcaa acggtacaac aggttcaaag
     1201 cggtatcgat gtcggcggtg tccgcccagg taagcagctt ctcactgctg accatttcgt
     1261 ccacatccgc tttcaacagg ctgtgcaaca aagttttacc ggcactgggg gcatcgctgg
     1321 atacggcata aaatgcaccg gcaggagtca ggcggggata taaatttgct tgtagtgaaa
     1381 gtgttgattc catattaaac ctccagtccc ggatcaatag aaaataacat tgcgctaacc
     1441 aattgtttta cgtcatcttc cttacgggca tcaatttcaa aaaccggaac attaagatta
     1501 tgttttgcaa gatatttgtg atacacgtcg ataccgggct gagagcgtat atccatcttg
     1561 gtaataccga caacgacggg tgccttctcc agcagccctc gaaacgaatg taaaaagaat
     1621 tccaaatctt tcaacggatt ggttcgggca ttatctaaaa gcaagaccaa acccatactg
     1681 ccttggctta agatttccca cataaagttg aaccgttcct gaccgggcgt accatataaa
     1741 tggactttgg tatcctcatc caagctgatg gccccgtagt ccatcgccac tgtcgtattc
     1801 cttttcctat ccaaagtcat atcggatgcg gaagcatcgg tctgaacgag tgcttcgtcc
     1861 gaaatagccg caatggcagt ggttttccct acgccgacag gtcctgtgaa aataatttta
     1921 ttttctctca tctctccgcc tcttagctgc ccaacagttt tttcatcagc ctttgcagaa
     1981 gaccgcgcga ctgggattgg gatggtgttg tgattttttc cgcttttttc atcatttcac
     2041 tatcagaggc agaatcggct ccgatattta ttttatccgc catatcggag tatgcctgtt
     2101 gtgaaaccgt ttttaaatct accgacaaaa acccggttgt ataggttgcc gcaagataat
     2161 tcagaatatc attgaggttt aaaggcatca ctttatacaa cacgttaagg ttgacggatg
     2221 ccttggtcag aaatgccgac aagcgtatcg accccggcac atttgccaac cgggtcaggt
     2281 ttggccaaga tttcaacgta aacggagtat cgggagaaat cggataaatc aacctgccct
     2341 gcgccgtcca aatggaaaac tgccacatac aggacataat gcctacttta gccttttcgc
     2401 gccattgcgg gttatcggga acagtcttgc agctgacctg caaattttcg tctttgcaca
     2461 attcttcgag tttttgcaca ctttctgtca gcaaaacccg ttgtatcgag gggaaaacaa
     2521 taaggaccgg cttatttcca tgcaagatag cgatgtcctg cctgttcttt tccgcaaacc
     2581 gcaacgcccc caataatcct ttattcgggt taaactggcg tatcgtaacc gtacgctgca
     2641 cattcccgtt atttttcgcc gaccggtctg cgggtgcaat aaacgatttc ccataaacat
     2701 tctcgccctg caacaatttg cggagcatag gaaacaatgt ttcaaaccga atcggtttgg
     2761 gcaggtaggg aacttcagaa tcgggaactt tctccgaaca gacggcgacg ggtatatcct
     2821 tataacgctc ggcaagctct ttccaaagtt caaaaccgcc ctcggcatcg gtatccgcca
     2881 aaaccaaatc gggcacggca ctgccgtctg aaggggatac tgtttcataa cgggtggtat
     2941 tgtgcatttt gaatgccatt ttgaaaacgg attcctgctg cgccgtcatc cccgccaaca
     3001 ttacgcgtac tgttttaatt ttcggcagtt gaacttccat ttttattttc cgtaccgttt
     3061 tattttttta atattttgat tcatacgctg cagcagccgg ctcatcagca taacgacctc
     3121 ttcaggaagc ctgtccgcac gttccctcaa tacccttaaa aactgcccca acctatccca
     3181 atcttcagta cgttcataaa tatcgatcaa cgtaatataa agctgggact cgtcgggata
     3241 tttcaatacc gcctgctcca acacatccat tgccgcttca atctgaccat acatcagcaa
     3301 cgactctact tccttaaccg catcgtctgc cggagacgaa ccggtgttga tcaacgaaga
     3361 atcttgaagc accaaatccc gatgttgcga tttgaatttc tgtatatttt tcggcagata
     3421 cccgtgcccc ataccgatgt ctttgatttg ccggtcgttc ggcccttttt ccaaatcatc
     3481 gaaaacttca tggtaaccca agctgtaccc ccaccccagc atccgctctt taacctgcct
     3541 gccgtagtta cccaacgttt ggtaaagttt ccacaaatgt ccggcaaaac ggtctatgtc
     3601 cgcgtgttgg taatcgagtt tcaacgcatc gataatcagg tttgcaggtt tttcggaagt
     3661 ttggatggca cggtcgtatt gtttcgatgc cgtttcatag ctgactttgt ctttaaggat
     3721 tttcgcacct tgatcggcgc gaaccaaacc ggcaatcgca ccgatttcct cttgactgat
     3781 tccggacacg tcttttttgc cccgcacaat cgggatgcgc ttgatttctt cggcttcata
     3841 agccttaccg ccggcatccg gcgggggcga tgcggaagct gccgtcgcag aaccgccttc
     3901 cgcacgtttt tcaatctcct gagtgcccca tcccaaacct tcttccgcca agacacggat
     3961 acgcaaatgg tttgaatcgc gctgtaatgc ctgttcgata tattttgcca aaagttcgga
     4021 aggaatcagt ttgccgtatt tttgcagatt gtctgccaaa acatcgacat cccccacttc
     4081 gagattgata tcgagcagct cgcggataag gttttcaggt ttcgcttcac catccggaat
     4141 gccgtccaga taggcagcca aagattcggc agccttgccc tgataaccga attgcttata
     4201 aacctgatac tccgtaagcg gatcgacttc ttgcgcggat acggcggcgg atgcggtctc
     4261 ggcactttcg ttccaagacc agtcgggttg cgtgccgtct gaaaccgatt gtccaatctg
     4321 atcgacccag tccgaatcat tgcttccggg tgtctgcccc tgcgtattgc cgacaccttg
     4381 ggatttgcgc ttcggtgttt tcttaccctg cttcgcacgc ataaccaaaa gcatcagcaa
     4441 taccgccagt gccaaaccga taattaatga gttttccaaa ggatactccc gataccgtaa
     4501 cgagcggata gctgaccgcc tgcaacggcc attgtccgct tttattatta acgatttact
     4561 gaaatagact tgtaagtttt aaatcatacc ataatttaac gtttaacaat atgccttctg
     4621 cacaagcctc gccatattac cttttaccca catcagtatc aatacccgat ataaaaataa
     4681 ctttgcccat aagctgcctt attgcctgcc ctccgcagag tagcgcgcga taaaaaataa
     4741 cggatgataa gtcaggcgca ctttgcccga cggcatactg aacgccgaca agtaatcgcc
     4801 aataaatcca ttgagatttt ttcttgtcca atttttttgg ttcgtggcgg taacgcctgt
     4861 atatttaagg tgtttgagta catctaacgg cgtatcaaag tctagtatta ccttaaaatc
     4921 ctcacaccat aaaagctcaa aatctttggc taaccaagtc tgccattggg ataaagtcgg
     4981 gtaatttaag cctatatttg taatttggcg gacttctttt aaattgtctt tgccaaaggt
     5041 tgcaaccgcc aataatccgt ttgctttcaa gcctgttttg caatgggtga taaaagcgtc
     5101 gggttgatga aaccattgca cggcagatgc gcttgcgatt aagtcaaatt gtcgttgaaa
     5161 aggaaagttt tccgcatcgc cgcaataaaa atcaaaggat tgcggcagtt tttcagccag
     5221 ttggggctgc acatcgcaca aatcattaaa taaccaataa ttcgctgaaa tctgtttttg
     5281 cagcaaggca ctcaacattc ctgagccaca gcccaattcc aacacgtttt ccaatggcat
     5341 atccggcaaa taatcttgca aatgcgccat taaattaatc gtcatttttt gttggattaa
     5401 ggcgtgccgg tcataatcgt ttaatgcttt ttggaaagcc tgccgaatgc gcgatttatt
     5461 tatggcagtc agtgattcca tagtgccgac caatgggtga atcttgaaaa caggtaatgt
     5521 ccgccgtcaa tttcccgaac ggtgcaacgc ggtgtccaat atcggtgctg attggcaggc
     5581 ataaaaattt tatcgcccga tccgaccaag gcatttgtcc agcggataag atctgtacac
     5641 ctatcttgcc cgatcatctt aaaaagtgag gtaagttctt gatgaatttc gtcaaacggg
     5701 cgggcgggaa attgttggta atcttcaaaa gatgctttat cgccacagat tctgcgttca
     5761 aattttaaac gggtatcttc cgtgaggttt tccaatgtgc ctttaaaaac ggcgaaaggg
     5821 ataccgaaat tatcatcgca aggcaaacct gtgccattca ctgccgttgc ggattttaat
     5881 cttattcctt gcaatgccct ctctgccgcc caaacgccca ttgaccacgc caccaaacgg
     5941 atgtgccgat aggcggaaaa atcaaaatcc aaatttacat cttgataatc atagcaaatc
     6001 aataaatcgt gattttccgg caaaatcaaa tgatttacag catcgggcgg cgttccccaa
     6061 cctgcaaaat acaggattaa atgtccgcct tgatgattgt aaaattttgt tttcatatca
     6121 tatccttaca ggcacgccgc aaactgccgc acttcatccg ttgtcatatc tgccgttaaa
     6181 gacaggcgga ttctggatgt gtttttgggt actgtcggcg gtctgatggg caggcaataa
     6241 taaccctgcc tttgcaggta ttccgctttg gcaagggcgg cttcattccc gcctaaaata
     6301 taggggacga tgcaggtttg gctcggcatt atttgcgtcc gatgcgccac ttcccgccgt
     6361 aaaaatgcac ttaactgctc aagatggctt ctttcttttg agaattgcgg caatcgttca
     6421 aaaataaaat aagtccaagc cacattaaac ggcggcaatg cggttgaaaa aatcaatggg
     6481 cgcatttgat taatcaaaca ttctttcaat acttggttgc agacggcata cgcccccacc
     6541 gaggctaagg ctttaccgaa agtgccgacc aataaatcaa tctcggcaat cagattatcc
     6601 cgttcggcaa tccccaatcc gttttgccca taaacaccga ttgcgtgggc ttcatccaca
     6661 taaagataag tattgggaaa ctgttttttt aattggacaa gctgtttcaa atccgccaca
     6721 tcgccgtcca tactgaaaac agattcggta acgataaaag tgcggtcaaa ttttccgacg
     6781 tttttttcaa gcagattttt caaatgttca taatcattat gacgataacg gaaaaacgca
     6841 caccggctca aacggatgcc gtcaatcata ctggcgtgaa caaatttatc tgccaaaatc
     6901 aaacttttcg tcgtcgtcaa agcaggcaaa ataccgagat tggcgtgata gccgctgttg
     6961 aacaataacg cgctttcccg ttggaaacgt tgtgcgacaa gctcttccaa atcggtataa
     7021 ataggaaagt tgcccgttaa taaacgcgat gaagaactgg taaaagaggg aaaattaccg
     7081 ccgtattgct gcaaaaaaga ccggcgcaag ttttcatctg atgccaaacc caaataatca
     7141 ttagacgaca tattcagcat tttgcggttt tcccgcgtaa tataccgccc ttgatgaatc
     7201 aaatccggaa tcgaacgata ttggttttgc gcgccgagtt gttcaagctg ttgtttaaaa
     7261 accttcatta tgatgtaaat attcctgagt taaagcctga accaacccag catcaaacaa
     7321 tcaagtaatg cccttttaat aaaaaccgtt ttgccgccga cctttgccac acccctgcct
     7381 tcataatcca aggcggaaat ttccgcgaca tttgtttccg ttgccattac ccaatccgtc
     7441 cgcactgtac cgaagcgcat attttcgcac aaattggtac ggtttcaggt atgatttgcc
     7501 gaaaacctat cggacacaaa ccatattttg atatgaataa aacaatctgc cgcactgctg
     7561 cactattgat aagcggtttt tcatacgcaa acaccgtcat tcccgatgtc tcccccgtcg
     7621 cacaaggtca acatgtcttc attaacatcc ctcagcaacg cctgttcctc tacaccgacg
     7681 gcaaactgac caaggtttat cccgttgcag taggtcgggc gatgacgcaa accaatctag
     7741 gcgaacataa aatcggtgcg aaagcctata accctgtttg gtacatcccc aaatccatac
     7801 agaaagaacg cggcgacggc gtaaaaacca tagccgccgg cccggacaac cctttggggc
     7861 cggtttttgt ccgcttaggc gaccctaaac tcggtttggg catacacggg accaatgcgc
     7921 cggccagcgt ccccggggtt cggagtcacg gctgcgtccg catgaaatcg cccgacgcgc
     7981 tcgagtttgc caaaaccatc gccagcggct cgcccgcctc cgtcatctat caaatggcgg
     8041 gtctcaatga agatgcggat cgcaacctgt ggcttgccgc cttccgcgac ccttacggta
     8101 agaacaacct tgacatcgcc tctctgaaaa aaagtattgc gcaatgggca aaaacacagg
     8161 gtaaaaccat cgcgcccgag aaagtcgatg ctgtactcaa agaccgcacc ggatcggccg
     8221 tctgcctgac ctgcggcaaa aacggcaaga tgaagatgcc gctcaaatcg ctggcgtgga
     8281 tacagggttc ttcctcatac agccaagctg aagtcattga acaaactgag gaaaccaact
     8341 ctgccgaaat atccgagaca cgcacgcccg aagtgcctga tgtacacacg cccgaggcac
     8401 aaccgcattt aaatacccaa tccgacggca cgccgactgc ctataccgaa ccggctgccg
     8461 attcatcgcc gcaagtagaa acacctgatc aggctgcttc cgagccggtt gatgtattat
     8521 tttcaataga tgtgatacgg cagggaaatt tgcgtttagg taattgaata accttctgat
     8581 tatttaatgc ttatgtttat cagaagttga taggcggttt ggtttttgtt tggcttttcc
     8641 tacaccgccg cctgccgctt tctgcaacat tcaagcgcac aaatatgcca tctgaaggct
     8701 ttagacggca tatttcacga taatagtcgc ccaatcaaat aaacacagat tggaaattta
     8761 ttttattttt cccttaccaa taccccagaa ctttccacca aatgctgccg ataacgaaaa
     8821 aaatcagaaa attgaccacg ctcatgataa aacccgcctt ccaccattct cccattgtgg
     8881 tgtagcccga accgaaaatc acaggcgaag taccggtcgc ataatgagtg agggtcatca
     8941 taatgttgga tgcggccgcc atcatcagcg cggtcggcat cgccggggca ttcagtgaaa
     9001 cggcagcagc gaaaaatgcg ccgaacatag cggtaatatg tgcagtagta ctggcaaaca
     9061 tataatgcgc atacatataa gcaagcacga ggattacgcc cgcagccgtg ccgctaacgc
     9121 ccaaaccgcc gacactttcc gccaacactc cggagaacca tttaatcagt ccgagtttat
     9181 ttaaaaatgc agccatcata atcaatgcgc caaaccaaat aatcgtatcc cacgcgcttt
     9241 tttctttcaa aacatcgtcc caagtcaata caccggaaag caaaagcagg cttaatccga
     9301 taaatgcggt ggcggtggcg ttgatactaa aagcgtgatt gccggtaata agggcgggaa
     9361 catctgccca caacagcaac aagataccga aaatgaccgc cataatgatt tcgtctgccg
     9421 acattttacc catctccctc agacggtctt tggcaaattg aacggcattg ggcgtttctt
     9481 taatttcagg cggatacaaa aaatataaaa tcaaaggcat aacgaaaaag gcgataacgc
     9541 cgggaacagc cattgcccac gcccacgccc cccaagaaag acggaaacta ctgcctaaat
     9601 tttcggcaat caagttgacg attaaagggt tgggggcagt tgcagtaata aacatagccg
     9661 acgaaatggg attggaatga tagttgacca aagccaaata tttacccatc ttgccttctg
     9721 tgccttttgc gggattggag ccgtaactgc cggcaatcga ctgcataatc ggatgtataa
     9781 tgccgccgcc gcgcgcggta ttggaagggg taacgggagc cagcagcagt tcggaaagag
     9841 cgagactgta accgatgccc agcgtttttc ttccaaaaac ggcgataaac aaatatccga
     9901 tacgcatccc cagccctgtt ttgagcaaac cgcgcgaaat cataactgcg atggcaatca
     9961 gccaaatcaa cggattggcg aacgcactca acgcatcgct catcgccgca cccggtttgt
    10021 cggcggttac gccggttact gcgaccaacc cgacggcaat aatcgacagc gcacccaacg
    10081 gcatggcctt gccgataatg gcggcaatca caccgataaa catggccagc agcgtccaag
    10141 cctgaggctt gaccccgtcg ggtacgggca gtgccaaaac caaggcgcac aatactgcgg
    10201 caatggcgag gggtatcggt ttgaaaccca atttcatcat attgacctcc gtaaaaaaga
    10261 ccgtcccgaa aaatcggaaa aataatattt aactaattgt tttataatat atattccgat
    10321 atttcaccgt ctttccgata tgcggctccg ggcaactttt gtttcagtat ttgaattttc
    10381 attagactga atacgccgtt tgaacggcac ggcgaaaacc cgggggatgc cggacgttca
    10441 gtcctttttc gcaccttgaa ggtaaagcat ttccaaggca attgtggcac cggccaaagc
    10501 ggtaatgtcg gattggtcgt aagagggggc aacttctaca acatccatac cgacgatgtc
    10561 gagatccgtc agcccacgta ggatttttaa tgccctgtcg ctgctcaagc cgccgcatac
    10621 gggcgtaccg gtcccagggg cgaacgacgg gtccaggcag tcgatgtcga aagtcaggta
    10681 aacgggcata ttgccgacgg tttctttgat tttacggacg gtctcttcaa cactgtcttc
    10741 attgacttgg ggggcggtca acacagtaaa aggcaatttt ttactgtgtt cggtacgtat
    10801 gccgatttgt acggaacggg acgggtcgat gaggccttcc ttgggggcgg tatagaacat
    10861 cgtaccgtgg tcgtattcgc tgccgttgtc gtaggtgtcg gtgtgcgcgt caaaatgaat
    10921 cagtgcgagt ttgccgaaat agcgggcgtg ggcgcgcaac aacggtaggg taatgaaatg
    10981 gtcgccgccc aaactcaaac agcgtttgcc ggaagaaagt aatttgccgg cgtgcgcttc
    11041 cattttttcg acaaaatccc tgctgtcgcc aaaagaaaaa accaagtcgc cgcaatcaat
    11101 aatgttcagg cgttcgcgca catcaaatgt ccatggaaac ctgcggtgct cccaagcgag
    11161 gttgacggag gcgcgccgga tggcttcagg accgaaacgc gcgccggaac gccctgaaac
    11221 cgccatatca taaggcacgc cggtaataac ccaatcggca tgactttcat acggcataaa
    11281 attaagcggc aggcgcaaaa acccgaaatt attggaaacg agggagttgt cggtttgtcc
    11341 tgccagtgtg ctgtattgca tcgtaatgat tccttgtaat tggtttcaat cggtcgtgat
    11401 gattggtgtt tgagtaagaa aatcgggctt cagacgacat atccgatgcc ttgatgcgtc
    11461 tattcgtctt ccaaataggt ataaccatta agccccgctt cgagttcttt taagaaagac
    11521 atagcctgcg aggcaggaag gtctgaatgt tcgatttgtt cgcgatagcg tttcatcagc
    11581 tctttcggat cttgataaac gtattcgagc atatcggcaa cggtgtttcc ttcatcgtaa
    11641 tcgatgacgg taaattgtcc gtcttcccct acaacaacat cggcagtggc agtgtcgccg
    11701 aaaagattgt gcatattgcc gagtatttcc tgatatgctc ccaccataaa aaagcctaaa
    11761 agcggcggct cttcttcggg ataatcaggc ataggcatcg taccggcgat gccgtcgccg
    11821 tcgatgtagt ggtcaatcgt accgtctgaa tcgcaggtaa tgtccaacaa cacggcgcgg
    11881 cgcgcaatcg gttcattcaa accggtaatg ggacaaacag ggaaaagttg atctatgccc
    11941 caagcatcgg gcaaagattg gaagagtgag aaattgacat acagcttatc ggcaaaacgt
    12001 tcttgcaatt cgtcaataat ggttcggtga gaccggtgtt tttcattaaa caattcgccg
    12061 acttcatgac agatatttaa atacagttgc tccgcccacg cacgttgcgc caaactcaac
    12121 agcccgacat tatactgatt atgcacatcg gcaagatcaa actgcccttc gtgtatccag
    12181 ctgcgtaagg aacgtttttc ccgcgaggcg gaaatatccg tccaagtttc ccacatactg
    12241 tgcaacacac gcggtgcttc gggcgatggc gcatccagcc ggcgcggttt gtaacgttca
    12301 acgcctataa cattagcaac caaaacggcg tgatgtgcgg taatgccgcg cccgctctcg
    12361 gtgatgattg tcggatgcgg cagcccgtgt tcgagacaag cctggctgat gccccatacg
    12421 actgtggcgg catattcgtt gaggctgtaa ttaacggaac aatccgattg tgtgcggttt
    12481 ccttcgtaat ccacgccaag cccgccgcct acatcaaaac agcggatatt tacccccagt
    12541 ttgtgcaact caacataaaa ccgagccgat tcgtgtacac ctgtggcaac atcacggatg
    12601 ttcccaagct gcgagcccaa atggaaatgc aaaagctgca ggcagtccag cctgtttttt
    12661 tgtttcaaaa tatcgaccag ttgcaaaact tgggaagccg acaagccgaa ttttgatttt
    12721 tccccacccg aagactgcca ttttcccgaa ccttgggaag ccagtctggc gcgcacaccc
    12781 aaacggggct tgatgccgag tttttccgcc tcttccaata ccatttgtat ttcggacagc
    12841 ttctcaatca ccaaataaac ctgatgcccc agtttttcgc ccatcaaggc gaaacggata
    12901 tattcacggt ctttatagcc gttgcagacg attaatgttt gccgggtgcc ggcgtgtgcc
    12961 aaaaccgcca tcagttcggc tttagaacca gcttccaaac catgcggttg tccgcttgac
    13021 ataagcgatt cgatgacgcg gcggtgttgg ttgaccttga tagggtaaac caaacaataa
    13081 ccgcccttat agccgcactc ttcccgtgcc gtctgaaagg cgcggttaat gtcgcggagg
    13141 cggtgttcga ggatttgcgg aaaacaaaac aaaacaggca ggcgcgcctg atgtttttgt
    13201 tgcacggctt cagtcagttt ttgcagtgaa acagtttgat tgtgttgcga gggattgggg
    13261 cggacgatga tttcgccgga atcgtcaaca tcataataac ctatgcccca atgattaatg
    13321 ttgcacactt cacggatggt aaggataggc ataataaacc tgctccgtct gtcgtgttga
    13381 aaaggaatga ttataacaaa tcagcgtgaa atgtcatttt tttaataaga aaagcctgcc
    13441 tcatacctga tgagaaacag gcaaaatgcc gtctgaacgc ttcagacggc attttggttc
    13501 agctttccat caaaggaatc agcgatcgag ctgctctttg atgattttca ggtcggggta
    13561 agacaacacg atgtcgtatt cgcggccgtc tttataggct tccacttcca aaacaggttt
    13621 gccccaatgg tcgtcggcat cgacatcgtg aacctgataa ccgcgctgtt ccaacatttt
    13681 cacggctttt gtgcggtttt gttcaaaatg gggatcgccg taaatctgac gctcggcaga
    13741 gtcgccggca aatgcggctg cggcactcag ggaaacaacg gcgaccaata acagtttttt
    13801 cattttcagt ccttttttta tcggttgatt gaacaagatg tgtttttcaa taccgccatt
    13861 aaaacacagc aaaattaggt ttgaattaga gggaagtcaa gaagcgtaaa tgccgtctga
    13921 aaaaaacaga gccgtcaaac ggctctgttt tccttatgct tctttggttt cagccgcctt
    13981 ttgacgcagg cgcaaactca attcgcgcaa ctgcttgtcg tccacgctgt tgggcgcgtt
    14041 ggtcagcagg cattgggcgc gttgtgtttt cgggaaggca atcacgtcgc ggatagattc
    14101 ggcgccggtc atcagggtaa ccaaacggtc gaggccgaat gcaaggccgc cgtgaggagg
    14161 cgcaccgaat ttcaggttgt ccaagaggaa gccgaatttc tcttgttgct cttcagggct
    14221 gattttcagc gcggcaaaca ctttctcttg tacgtctgca cggtggatac ggatagagcc
    14281 gccgccgatt tcccagccgt tcaataccat gtcgtaggca cgggccaggc agtttgcagg
    14341 gtcggaaacc atcaggtctt catgaccttc tttaggcgcg gtaaacggat ggtgtacggc
    14401 aacgtagcgg tcggcttctt cgtcgtattc gaacattggg aaatcgacaa cccacaaagg
    14461 tttccattcg tctgtgaagt agccgttatc tttgccgtgt tccaagccga ctttgatacg
    14521 caatgcgccg atggcttcgt tcacaacttt ggttttgtct gcaccgaaga agataatatc
    14581 gccgttttgt gcgccggtac gcgcgatgat ttccttcagg gcattctcag acaggaattt
    14641 tacgattgga gattgcaggc cgctgtcttc gccgttggag aggttgccca catcatttac
    14701 tttgatgtat gccagacctt tcgcaccgta gatgccgaca aatttagtgt attcgtcgat
    14761 ttctttgcgg ctgaatttcg taccgttcgg cacacgcagg gcaaccacgc ggccgccttt
    14821 catgtcggct gcgccacgga agactttgaa ttcttccgtt ttcatcaggt cggtcaactc
    14881 ggtgaatttc aagttgatac gcatgtccgg tttgtcagag ccgtagtaga acatggcttc
    14941 agagtaaggc atgcgtggga agtcgcccaa gtccacgccc agcgcgtctt tgaagacttg
    15001 tttcgccata ccttcggtga tgtccatgat ttcatcctcg tttaagaacg aggtttccaa
    15061 gtcgatttgc gtgaattcag gctgacggtc ggcacgcaag tcttcgtcgc ggaagcactt
    15121 ggtgatttgg tagtaacggt cgaaacccgc caccatcaac agttgtttga acaattgcgg
    15181 cgattgcggc agcgcgaaga actcgcccgg atgaacgcgg ctcggcacga ggtagtcgcg
    15241 cgcgccttcg ggcgtggagc gggtcagcat cggggtttca atgtcgatga aaccttgcgc
    15301 gtccaagtag cggcgaacgc ccatagcgac ttggtaacgc aggcgcaggt tgcgctgcat
    15361 caccggacgg cgcaggtcga taacgcggtt ggtcaggcga acgttttcgc tgatgttttc
    15421 gtcgtcgatt tggaacggag gcgtagcggc agcgttcaac acttcgattt ctttggcaag
    15481 gatttcgatt ttgccggaaa tcattttgtc gttggtcgtg ccttcgggac ggttgcgtac
    15541 gcggccggta atgctcaaaa cgtattcgtt gcgggaggaa tcggcagcgg caaacgcttc
    15601 gggcgtgtcg ggatcgatca cgacttggac gatgccttcg cggtcgcgca ggtcgataaa
    15661 aatcacaccg ccatggtcgc gtcgacggtg tacccagcct ttgacggtaa cggtttggtc
    15721 taagtattgc tcgctaatca ggccgcaata gttggtacgc ataaaatcac cttttattga
    15781 tttaaactga aaacagaaaa tgccgtctga acggcggctt tattgttgtt cgggcaaatc
    15841 cgccttttca gacggcatag gtcctgccaa tgttttgacg ggcaggtcgt cagggatgac
    15901 catacccagc gaaatcacat atttcaacgc ttcgtccacg ctcatatcga gttcgcgcac
    15961 atcacttttc tttaccataa tatagtaacc gccggtcgga ttcggcgtgg tcggaacata
    16021 cacggaaaga taatcgccgt ccttcggcaa tgcggcctta accgcattcg acacctgacc
    16081 ggacacgaat gcgattgtcc aaatacccga ttggggaaac ggcacgagta ctggtgtttt
    16141 aaacgaacgg ctgctgtcgg acagcaacga ttcggatact tttttcacac tcgaatagat
    16201 ggacttcaca accggaatcc gccccaacag gctgtcccac gcggcaagaa tctgccggcc
    16261 caatacgttt gcggcaaaca atccggttac aaacaatacg gcaatggcaa caataacgcc
    16321 cagccccggg atattaaacc ccaaaacata ttgcggccgc cattgcttcg gcagcaggtt
    16381 gacaagctgg tcggacgcgg aaacgatata ggaaaccacc caaaccgtta ccgcaatcgg
    16441 cagccagacc aaaatgcccg taatcagata tttttttaac gccttggcag ctttgccgcc
    16501 ttcggccgca ggttccgtca tcttgcttga ttccgacaaa gtccgtacaa accgcacatt
    16561 atacgcgttt gcccggattc aaacgaaatt tttatcccgc cccgccaaac cgccggcggc
    16621 ttcagacggc acggcaactt gatatatcgt ctgaacacgc ggttcagatg ccgtccaagt
    16681 cgttgaacat caacccgata ccgataccgt tctgcttgtg gttgtagtcg atcaggctct
    16741 cgccgtaacc gtggaatccg cgtaccacgc ctttgagttt gcccttaatc ggaaacgtgt
    16801 aggcggcttc aatcgcgccg tagcccgttt tggggttgta gcgcaatacg gaatacacat
    16861 tctgcctgtc gttcaggcgg tactgcagct tcacgtcgcc ataccccata tagtcggcaa
    16921 tatcgggatt gtcgttttta tcgccgctct gatcgaacgc acgcacccac acgcgcggaa
    16981 tcaccgtcaa tttgccccat tccatgcctg ccatggcgta aatcctgttc cacgaacgcg
    17041 attcgggacg gctctgtccg ttggactggt ggacaaaacc cgcaccgagc atacgcagcc
    17101 tgccgccgaa cggcaaatcc gccttcacag gctgggtcag gaaaatttca ggtttgtaat
    17161 ccgtattgcg gaacggcgcg gatttcctgc cttggttgta aatctgccaa tcggatcttt
    17221 gggtgtagcc gaaccacaga tccgcgcggg ttttaaacaa atcttcggca attttgcttt
    17281 tgaacgaaac ctgcaatttg gtttccgcac gtttctgctg tccgaatttt tcctgtacag
    17341 tcgtaccgcg cgtcggcgaa cccggggcat agttgggcga attgttgtac cagagcggca
    17401 taaggtacat cggattgtgt tcgcgtacgc ccaacagccc gcgcaaatcg tttttgtcca
    17461 agtcgtacat caggctcaaa ggcgtataga tgtcggcggt ttcgcccgca ctgtcggcag
    17521 gaagcgcatc cccgcctttt tcaacaacaa tgaccgcctc gcccttatcc aggctgctgc
    17581 ggacggtttc cgtcagattg agtacggctt tcgactcctg cccttcctgc cctgccgaag
    17641 acggaagctg tgccgcaaaa atcctgtcgt aacacgccaa acgcgtaaca ttgtccgtca
    17701 aagcggcgca ttgcagcgcg gtctctccaa aagcggatgc catcggcaac agtcctgtca
    17761 aaagaatata gcgcatattc cgtgtattca tctccgcccc cattgtcggc atattggttt
    17821 tcaaacggca ttttatagcg gattcggata aaaaccgcac cctttccgcc atttcgggat
    17881 tttgcccccg caatacagaa aaccccgaaa ccgtcaggct tcagggtttt ccgcttatcg
    17941 cgtatcaacc gccttggcgg ttttgcaaaa atcaagccag tgcttttact tttgcagaca
    18001 gacggctttt gtggcgagcc gctttgtttt tgtggaacac gcccttgtcg gcgatgcggt
    18061 cgatgacttt gacagactct tggtaaaccg cttgtgcggc agctttatcg cctgcttcga
    18121 ctgctttcaa tacttttttc actgcggtgc ggaatgcggt acgcaggcta gcgttgtggg
    18181 cgcgttgttt gacggactgg cgggcacgtt tgcgtgcttg tgcgctgttt gccatattgg
    18241 aatctcctgg aagaaaaata aattctgtaa acgcgcaatt ttaaaggggg tttacattat
    18301 atgcaagcct tttctctcta tccgcccgtc ggacaccctg acagcgcaaa aaaacggcaa
    18361 aatcgtgcgg acgttttccc cctcttttga aaacagggta attttttagg gaaatcagtc
    18421 caatatcact ttgagtttgc acaaatttac tttacaatgc ccaaaccttg cgccggggtg
    18481 tttatggggc gcggggtttt ctttttttcc aacctctttc atcaggaatc tgcccgtatg
    18541 aaaaaatccg tattagccgt tttggccgcg ctgtctctgg cagcctgcgg cggcagcgaa
    18601 aaaaacgccg tgcaacctca agcagacgct gcgtctgctg ccaatgccga agccgctgcc
    18661 accgatactc tgaacatcta caactggtcg aactatgttg acgagagtac ggtcgaagac
    18721 tttaaaaaag ccaacaatct gaagctgact tacgacttgt acgaaaacaa tgaaacgctg
    18781 gaagccaaaa tgctgaccgg caaatccggt tacgatttgg ttgtgccggg catcgccttc
    18841 ctgccgcgcc aaatcgaggc gggcgcgtat caaaaagtca acaaagacct gattcccaac
    18901 tataaaaaca tcgatcccga actcttgaaa atgctggaaa ccgccgaccc gggcaaccag
    18961 tatgccgtcc cctatttctc cggcgtgaac acgattgcga ttacggcgaa gggcaaagag
    19021 cttttgggcg gcaagctgcc cgaaaacggc tgggatttgc tgttcaaacc cgaatacacc
    19081 cacaagctga aatcctgcgg catcgccctg tgggacaccc cgagcgaaat gttcccgatt
    19141 ttgctgaact acttgggcaa agaccccaaa ggttcgaatc ctgaagattt gaaggcggcg
    19201 gcggaagtat tgaagtctat ccgtccggat gtcaaacgtt tcagcccgtc catcatcgac
    19261 gagctggcgc gcggcgacgt ctgcctggcg gcaggcaacg gcggcgattt gaacttggcg
    19321 aaagcacgtt ccgaagaagt gaaaaacaac gtcggcatcg aagtgctgaa tccgaaaggc
    19381 atgggcttct ggattgagtc ttggctgatt cccgccgatg cgaaaaacgt cgccaatgcc
    19441 cacaaataca tcaactacac gctcgacccc gaaatcgcgg cgaaaaacgg catcgccgta
    19501 acctttgccc ccgccagcaa accggcgcgc gaaaaaatgc ctgccgagct ggtaaacacc
    19561 cgttccatct tcccgaacga gcaggatatg aaagacggtt tcgtgatgcc gcaaatgagc
    19621 acggatgcga aaaaactgtc tgtcagcctg tggcagaaaa tcaaagtcgg caccaactga
    19681 tttgaagcat taaaaatgcc gtccgaacat cgttcggacg gcattttata ttggattgaa
    19741 atagaaatat ttatatagtg gattaacaaa aatcaggaca aggcgacaaa gccgcagaca
    19801 gtacaaatag tacg
//
LOCUS       DAXHPG010000021        19746 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXHPG010000021 DAXHPG010000000
VERSION     DAXHPG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19746
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.021"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            153..389
                     /locus_tag="ACFM6F_000542"
     CDS             153..389
                     /locus_tag="ACFM6F_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164011.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            361..780
                     /locus_tag="ACFM6F_000543"
     CDS             361..780
                     /locus_tag="ACFM6F_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164012.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKAAFAKQQAVID
                     KMEHDKAQALLLSAQNYARELELARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
     gene            677..982
                     /locus_tag="ACFM6F_000544"
     CDS             677..982
                     /locus_tag="ACFM6F_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164013.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(1027..1197)
                     /locus_tag="ACFM6F_000545"
     CDS             complement(1027..1197)
                     /locus_tag="ACFM6F_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFC6164014.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            1210..1428
                     /locus_tag="ACFM6F_000546"
     CDS             1210..1428
                     /locus_tag="ACFM6F_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164015.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            1425..1763
                     /locus_tag="ACFM6F_000547"
     CDS             1425..1763
                     /locus_tag="ACFM6F_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164016.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            1775..2041
                     /locus_tag="ACFM6F_000548"
     CDS             1775..2041
                     /locus_tag="ACFM6F_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164017.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKADAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            2038..2235
                     /locus_tag="ACFM6F_000549"
     CDS             2038..2235
                     /locus_tag="ACFM6F_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164018.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            2238..2744
                     /locus_tag="ACFM6F_000550"
     CDS             2238..2744
                     /locus_tag="ACFM6F_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HFC6164019.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     AEVEKKFG"
     gene            complement(2741..3253)
                     /locus_tag="ACFM6F_000551"
     CDS             complement(2741..3253)
                     /locus_tag="ACFM6F_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164020.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELAPGSFVYRLKTEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDVEVFSVGG
                     SRYGRLIDGY"
     gene            3265..4887
                     /gene="terL"
                     /locus_tag="ACFM6F_000552"
     CDS             3265..4887
                     /gene="terL"
                     /locus_tag="ACFM6F_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFC6164021.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAVSARRAKVFD
                     PIGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWRGVKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYQANKDEMERGAVTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIVIGGYQRSMG
                     RLYVVEAQIKKRLPDLIIEDVICLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFGNEIKSKWSR"
     gene            4887..6455
                     /locus_tag="ACFM6F_000553"
     CDS             4887..6455
                     /locus_tag="ACFM6F_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFC6164022.1"
                     /translation="MAKKDNKTKIQKPEAALQTDAAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDDGLLLRTRENPEGEALWPLGWVVHTQKSRSVRQARNGLFRSLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTTATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPVNRTTLAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            6442..7737
                     /locus_tag="ACFM6F_000554"
     CDS             6442..7737
                     /locus_tag="ACFM6F_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFC6164023.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDSYPTTAYKAPDGSLYTADKGFGYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            7847..8263
                     /locus_tag="ACFM6F_000555"
     CDS             7847..8263
                     /locus_tag="ACFM6F_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFC6164024.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            8494..9555
                     /locus_tag="ACFM6F_000556"
     CDS             8494..9555
                     /locus_tag="ACFM6F_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFC6164025.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPEGAALSADVFAQLAEK
                     DSRIAALSAQNGTPDLTRYAPVSLVRDLQNQVAALTAEREADKGGELITAALSAGKLL
                     PAQKEWAEGVLKQPGGFAFLSGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            9617..10126
                     /locus_tag="ACFM6F_000557"
                     /pseudo
     CDS             9617..10126
                     /locus_tag="ACFM6F_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10116..11261
                     /locus_tag="ACFM6F_000558"
     CDS             10116..11261
                     /locus_tag="ACFM6F_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFC6164026.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            11258..11926
                     /locus_tag="ACFM6F_000559"
     CDS             11258..11926
                     /locus_tag="ACFM6F_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFC6164027.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRTAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            12027..12374
                     /locus_tag="ACFM6F_000560"
     CDS             12027..12374
                     /locus_tag="ACFM6F_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFC6164028.1"
                     /translation="MDKELNPSIGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            12388..13443
                     /locus_tag="ACFM6F_000561"
     CDS             12388..13443
                     /locus_tag="ACFM6F_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFC6164029.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLRRRNPTTASGTLTVSGIAQSMLSDG
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADELGAAANVRDGEAQLMAAPAGVSTECR
                     LTVQGGTDRESDASLLARLLEIIRRPPAGGNRYDYKNWALSVDGVTSAYVYPLRRGLG
                     TVDIAITSADGVPSEETVRRVQAYIDEMRPVTAKNALVLKPTVTAVPVTVQVKLDGID
                     LDDAKRRIRTALKEYFDTLIPGDGLTVSQIEAAISNVDGVIDRRLTAPTANRAADTVN
                     RIEWFKAGAINVTEMPS"
     gene            13440..14000
                     /locus_tag="ACFM6F_000562"
     CDS             13440..14000
                     /locus_tag="ACFM6F_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFC6164030.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPSSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            14011..15984
                     /locus_tag="ACFM6F_000563"
     CDS             14011..15984
                     /locus_tag="ACFM6F_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
                     /protein_id="HFC6164031.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGAAADNAHIVFGDNTLRLHGANNRISYNNHDIFHKANKPRFAEDIKGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTQPGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPAGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDTA
                     AVGYEDHNERQRSALVSTWTDGDLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(16667..16969)
                     /locus_tag="ACFM6F_000564"
     CDS             complement(16667..16969)
                     /locus_tag="ACFM6F_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFC6164032.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIVEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(16941..17228)
                     /locus_tag="ACFM6F_000565"
     CDS             complement(16941..17228)
                     /locus_tag="ACFM6F_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFC6164033.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            17308..17913
                     /locus_tag="ACFM6F_000566"
     CDS             17308..17913
                     /locus_tag="ACFM6F_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164034.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            17892..18188
                     /locus_tag="ACFM6F_000567"
     CDS             17892..18188
                     /locus_tag="ACFM6F_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFC6164035.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            18185..18394
                     /locus_tag="ACFM6F_000568"
     CDS             18185..18394
                     /locus_tag="ACFM6F_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164036.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(18564..19646)
                     /locus_tag="ACFM6F_000569"
                     /pseudo
     CDS             complement(18564..19646)
                     /locus_tag="ACFM6F_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
BASE COUNT         4969 a         5264 c         5540 g         3973 t
ORIGIN      
        1 ttttacgaaa agatgattga gctgtggtcg agcaaaagcc gtgaggcaag cgaacgggct
       61 gatttggcgg cgtttgaatt tgcggagggc gaactggcca attatcggga aatgctgaaa
      121 cggcacctgc aaaccaaaag tgtggaatag caatgcgtat tttggatatt tttaaaaacc
      181 cggcgacagg caatgtgtcg cactcgaagc tgtgggcaaa cgtcgcctgc gcggcgggga
      241 cgtttaaatt tgtgatgttg cccgacccgt cggcggagat ttgggcggtg tatttgggca
      301 ttgtcggcgg ctatgccgtg gcgcgctcgt ttgtcagcgt caaacgtcag gaggtcgaga
      361 atgaatctcg tgaaactgct ggcgaataac tggcagccga ttgccatcat cgcgcttgtc
      421 ggcacgggtt tggcggtgtc acaccatcaa ggctacaagg cagcctttgc gaagcagcag
      481 gcggtcatcg acaagatgga gcacgataag gcgcaagccc tgctgttgtc ggctcaaaac
      541 tatgcgcgcg aactggaact ggcacgcgcg gaagcgaaaa aatatgaagt caaggcgcac
      601 gctgtcggca tggctttggc gaaaaaacag gcggaagtca gccgtctgaa aacggaaaat
      661 aaaaaggaaa tcgaaaatgt ccttactcaa gaccgtaaaa atgcaggcgg cggttgtatt
      721 gacggctttg gccatcacgg cttgcagctc tacaagcgcg ccctcggcta cggaaattaa
      781 ggttgtcgaa aaggcggtca tgccgacacc gcctgccgca ttgatggtcg cgccggtacg
      841 cccaaatccg ccgaaagacg gcaagacggc cacgctgttg gaacacgccg ccgagtttgg
      901 cggctatgtg gcggagttgg aaaatcaaaa tcaggcttgg cgcgactggg cgggcaatca
      961 ctcccgcaaa gtcggaaact gacaaaaaag cccgcgtagg gcgcgggctg agggtgaaag
     1021 cggattttat acctctttta caggggtagc ggcggtagtg cttttcagca aatcgactgc
     1081 gtgctgacag ttttgcttgc tggtgtagcc ttcgccctga gcgatgattt catggttggc
     1141 tgctttcaaa cgccaacggt attcgccttt tgcgtcttta tagatttcaa aatacataag
     1201 gtttctccta tgaatgagta cacgttttct taccgcttta acggcaagtc ctggtcattg
     1261 agcatttggg cggacaaccc tgaagaagcc agggcgaaat ttcgggctgc acgagaaaat
     1321 gcgcactatg acggcgaagt tgtagcaaag gtttatacat ttgtaaatat ttcgtgggtt
     1381 aagaaattgt acaagcggac aaaatattta atgggtatca aagaatgacc taccgtgaat
     1441 tagttgaacg tcagttggct gtgcgccatg ccgatttgga attgggctta agccgcgcac
     1501 gcgagcaaga gccgtttgtc attcatgttt ccgatctgtt ggataaggca ggcattgagt
     1561 acgcggtacg catggataag gattttcaga cgacgtttca ccttgaatat ccaattacga
     1621 actatgacac ctttaaacgt gcggtttggc aaactttggg ggcgtattac tgtgtttgta
     1681 atgatggtga tggactggag attgccagca atcgccctga cggttacgcc gtccgtatcg
     1741 tattcggcga tgtgccggtt taaaggggtt ttaaatggac tttgaatttg gtttcagaac
     1801 cctgtggccg attgcgacgg cggcattttg gttttgggtc aacggcattt caggccgcct
     1861 gaaagaggcg gacaagcgta tcgacgacct taaagaggag ttgcacgcgg tcaagctctc
     1921 ttatcacacc aaggcggacg ccaaggcaga cagcactaat attgcggcgg ccttggagcg
     1981 aattgaaaac aagttagaaa aagtaaacga aaaactggac aggaaagcag acaaatcatg
     2041 agcgacccga ttttggaagc cttggcgcgt attgaaaaca agactgatca aacgctgaaa
     2101 aatcagaaag aaatgcaggc ggaaattgcg caaatccgcc aagacacgaa acgcacggcc
     2161 attacattcg gcgcactggg cggcggcgtg attacggtcg gctgggaatt gcttaaagcg
     2221 aaaatgggac tgtaattatg gctcacccgc aagaaatccg tgaaaagtta cgtcggctct
     2281 atgtgagcgg cgagcaaact ttggaaacgg cggccttgat gtgcgaaatc ccgcagacca
     2341 ctgcgcgtgc gtggaaacgt gcggataagg aaaaaggcga cgactgggat aagatgcgcg
     2401 ccgcctacac tttggccggc ggcggtattg aggacttgag ccgtgcgatg ttggccggtt
     2461 ttatggtgca gtacaacagc acgatgacga tgctgcagga ttcgagtacc gaagatttgc
     2521 ccccgtccga ccgcgccaag ctgttggcca gcctggccga tgcgtttacg aaaaccgtat
     2581 cggccaatgc gcgtgtgatg ccggaaacgt caaaactggc gacggctttg gaattgattg
     2641 agttcttgat ggcgtttgtg caagaaaaac atcccaaaca tttgcctgcc tttgtggagg
     2701 tattggagcc gtttggggcg gaagtggaga agaagtttgg ttaatagccg tctatcaatc
     2761 tcccataacg tgatcctcct actgaaaata cttcaacatc tgactttaaa cgctctccca
     2821 atattatggt ttggcaacgg gaacaggaat atttatgaaa agcaccttca tacccattga
     2881 attgaagaat cgatttgaca ccctgctcgt aacattgggg acaaatatca tgaattggct
     2941 cgctgttcgc catctctgtt ttcaatcgat agacgaaaga accaggagca agttcataca
     3001 attggtagcg ttgcttctct gtagaccaat tttttactgc caccagttct ttttccaact
     3061 catctatacg gctaattgcc gccgtataag aagattgggc ttcaaataaa tcagtttgaa
     3121 taccagcaat gatttgatta agttcgatgg ttttttgcgc tacctctgcc tctgttttga
     3181 gctggttaaa cccttttaaa agtgatcctg cttcttttag gcctataaat gcgttagaaa
     3241 tgaatccagt cattataaat actcatgaaa acaaaagaat tcctcaaatc ccttgccgaa
     3301 ctggccgcca gtttgcgcca agtcatcgaa gcggaagtgg acggcttcga tgcgtcgccc
     3361 aaggctgttt ctgcacgccg tgccaaggtg tttgacccga taggcggtta cgaatatttc
     3421 gtcaacacct acttccccca ttatatccgc tcgcctgaga aatccgaact gcatgcgttt
     3481 ttattcagcc gtctgccgga gattatccgc tcccccaaag gggaaaatga ggcggtgggc
     3541 gcaccgcgtg gcgagggtaa atcgacgcag gttacccagt tgtttacgct gtggtgtatt
     3601 gtgaccggcc aaaaacatta tgccgttatt gtgatggaca gcatagacca ggcatacccg
     3661 atgctcgaag ccatcaaggc ggaattggaa tttaatccgc gcctgaaaac cgactttcct
     3721 gaagtctgcg ggcaaggccg tgtatggcag gccggtacga ttgtgacggc caatgacgtt
     3781 aaggtgcaag tggccggtag cggtaaaaag ctgcgcggtt tgcgtcacgg cccttaccgt
     3841 cctgacttaa ctgttttgga cgatattgag aatgacgaac aagtccgcaa ccccgaacag
     3901 cgcgacaagc tcaatgcgtg gctgactaaa acggtattgc ctttgggcgg ggtcggtcag
     3961 aaatacgatg tgatttatat cggcacgatt ttgcattacg acagcgtact taaccgcact
     4021 ttgaataacc cgttttggcg gggtgttaag tttaaggcga tgaaacgctg gcccgaccgc
     4081 atggatttgt gggacaggtg ggaggaactt ttccgaaacg acggcgagac ggtggccgag
     4141 gcgttttatc aggcaaacaa agacgagatg gagcgcggcg cagtcacttc ttgggcggcg
     4201 cgtggcgtac tcgcgctgat gaaaatccgt gcgcgtgacg gccatgcgac gtttgattcg
     4261 gaatatcaga atgatccggt cagtggcgaa gatgcgccgt ttgccaagtc gatgaagttt
     4321 tggaacgacc tgccgtccga tttggtgtat ttcggtgcgc tcgacccgtc gctgggtaaa
     4381 gcgggggcga gccgtgaccc gtcggcaatc gtcatcggcg gctatcagcg gtcaatgggc
     4441 aggctttatg tcgtggaggc gcaaatcaaa aaacgtctgc ccgacctgat tatcgaagac
     4501 gttatctgcc tgcaccgtca atatcgttgc aaactgtggt ttgttgagac ggtgcagttt
     4561 caggagtttt taaaagacga gctggtcaag cgcagcgcgg cgcgtggaat acctgtcccg
     4621 gcgcgggcgg tcaagccggt atcggacaaa ctcttgcgga ttgagacttt gcagcctcac
     4681 atggcaaacg gtttgattct gttgaatgag agccaacaga cgctgataca gcagttccgc
     4741 cattttccaa aggctgatca tgatgatggt cctgatgccg tgcatatgct ctggtcgggg
     4801 gcggtggcca attgtgtgcc gatagaatgg caaagcccta ccgataacga ttttggtaac
     4861 gagataaaaa gtaaatggag ccgataatgg caaaaaagga caataaaact aaaatccaaa
     4921 agcccgaggc tgcattgcag acggatgcgg ctcaaattac ggcgaccggt cgggttatcg
     4981 ccgagcatcc gtccaatttt attacgccgc aaaagatgcg cgccctcttc gaggacgcag
     5041 aaagcggcga catccgcgcc caacacgagc ttttcgcgga cattgaggag cgcgacagcg
     5101 acatcgcggc aaatatgggg acgcgcaaac gcgcgctgct gacgctcaac tggcgcgtcg
     5161 ccccgccgcg aaatgcgacg cccgaagaag aaaagctgtc cgaccaagcc tacgaaatga
     5221 tggacagcct gcctaccctc gaagacctga ttatggattt gatggacgcg gtagggcacg
     5281 gattttctgc gttggaggtc gagtgggtat tttcagacgg cctttacctg ccccgaaact
     5341 ttatccaccg cccgcaaagc tggttcaaat gggacaaaga cgacgggctg ctgctgcgta
     5401 cccgcgaaaa tccggaaggc gaagcgttgt ggccgctggg ctgggtcgtt catacccaaa
     5461 aatcgcgcag cgtccggcag gcgcgcaacg ggcttttccg ctcgctttcc tggctgtata
     5521 tgttcaaaca ctacgccgtc cacgattttg ccgagttttt ggagctgtac ggcatgccca
     5581 tccgtatcgg caaatacggc gcgggcgcaa ccaaagagga aaaaaacacc ctgcttcgag
     5641 cggtggcgga aatcggtcac aacgcggcag gcatcatgcc agaaggtatg gaaatcgagc
     5701 tgcacaacgc tgcaagcggt acgacggcaa ccagcaatcc gtttttgcag atggccgatt
     5761 ggtgcgaaaa atcggcggcg cggctgattt tggggcaaac gctgaccagc ggtgcggacg
     5821 gaaaatccag caccaacgcg ctgggcaata tccacaacga ggtacgccgc gatttgctgg
     5881 tgtcggacgc aaaacaggtg gcgcaaacca tcacaagcca aatcatcgga ccgttcctgc
     5941 aaatcaacta tccccatgcc gacccaaacc gcgtgccgaa atttgaattt gacacgcgcg
     6001 agccgaaaga catcgcggtc tttgccgacg ctatcccgaa actggtggat gtcggcgtac
     6061 aaatccccga aagctgggtg cgcgacaaac tggtcattcc agatgtgcag gagggtgagg
     6121 ctgtgttggt gcggcaggta ccggacaatc cggtaaacag aactacattg gcggctttat
     6181 ccgcccacac cgtaccatct aaggctacgg gcaggcatca ggaaatattg gacggcgcgt
     6241 tggatgacgc gctggttgag cccgatttca attctcagct caacccgatg gtgcgtcagg
     6301 cggttgccgc acttaatgct tgcaacagct acgaggaggc agatgccgca ctgaatgcgc
     6361 tttatccgaa tttggacaac gcgaaactgc gtacctatat gcagcaggcc ttgtttatca
     6421 gcgatatttt gggacaagac catgcccgcg cctgatttgg gatttgcctt aagtctgccg
     6481 ccaaaaaagg caatcgagtg gctggaaagt aaaaaggtta cggcggagag ctaccgcaat
     6541 ctgacagcct ccgaaattgc caaagtctat acgattgccc gcatgaccga cttggatatg
     6601 ctcaacgaca tcaaaacttc gatggttgaa tcggcaaaaa gtggacagtc gtttgacgat
     6661 tggcgaaaag gtatcttgaa tctgctcagc aacaagggct ggctgcatcc gaacgggcat
     6721 aacggtaagg atatcatcga cccagccacc ggcgaggtat tcggttcgcc gcggaggttg
     6781 gagacgattt accgtaccaa catgcaaact gcctacaacg ccggtcaata tcaaggatat
     6841 atggcaaata ttgatgcacg accttattgg atgtatgacg cggtaggcga cagccgcacc
     6901 cgtccggcgc attcggcaat agacgggctg gtgtaccgct acgacgaccc gttttgggca
     6961 acgttttacc cgcccaacgg ctacaactgc cgctgctcgg tcatcgcgct gtcggagcgg
     7021 gatgtggaac gccaggggcg gattgtcggg caaagcacgg cggataatct ggtcgagacc
     7081 cataaaatct ataacaaaaa aggcgacagc tacccgacga ctgcctacaa ggccccggac
     7141 ggcagcctct acacggctga caaaggcttc ggctacaacg ccgggcgaat gaactaccgc
     7201 cccgatttag acaagtacga ccgcgcgctg gcgcatcaat ttgccaaagc ggaaatgggt
     7261 ggtgcagatt ttaaaaccag ctttaaacag cttgaaaaag agttttatga agtcaagcaa
     7321 cgtttggata ttgatggcaa gcccgataaa gagcagaaaa tcaaaatccg aaatgcgcta
     7381 tcaagacagc ttaaatttgc tgcgggtgta ttgagcaagg aaacgcaaga attggcaggt
     7441 atgacacgag cgacggtgtg gctgtctgat gatacgttgg ttaaacaggt agacagccgt
     7501 gaggggcaga atttcgatga ctcctactat gcttttttgc cggatatgct gcaaaaccct
     7561 gaacatgtca tccgcgacaa tcgtgaattg attttcacag ctcgctataa aggctcggca
     7621 ttgtgggcag ttttaaaata tattaaggag gtggatgaga tttatctaca gtcgtaccga
     7681 atcagcaacg acaaagagat tgccaaattt atggcgaaga agaaagtatt gaaatagacg
     7741 ttgggcaagg ctcgaattca cttgcacacg ctctcgtaca ccctaaaggg caggctgcgg
     7801 tatcgagatt atcaccgctt ttccaacgcc tataaggaat cataccatga ttgatgtcaa
     7861 aatagacaat atctttgtcg tcctaaacca aatcgagcgg cttggcaacg ggatcgaaaa
     7921 ccgctacctg ctgatgcgcc gactgtccga aaccatgcac acggcggtca agctcaattt
     7981 ccgctacgca ggccgtccga aatggttggg gctaaaatac cgcgacggca agccgctttc
     8041 ggattcgggt cgtctgaaag acagtttttc cacactgtca gacaacgata cagcccttgt
     8101 cggtacgaat atcgtctatg ccgccatcca caacttcggc ggtatggcgg ggcgcaaccg
     8161 caaagttcgg attccgcaac gggaattttt gacgctgacg gacgacgaca aacaggcttt
     8221 gatggacgat gtgcaggatt atttttcggg tctgataccg tgaatttata aaaccctcaa
     8281 aaacgcgctt tttagcgcgt ttttttatgc gggtaataca aacccctgcc caagatataa
     8341 aaatcaatcc tagacgcttc taaaaagccc ctgaaaacga ttaattgtgt atcgcgcgga
     8401 caggttttaa aaaaatggcg ggagggtttg aagcacgcct actctttgtt gttttttcaa
     8461 ataggcaaaa tgaccgtatt gagagaggta cacatgtcca aaaatgcaca aaaaacccta
     8521 cttgccgtgt gcagtttcga ggtgcagcca aaagacgggc gaatccaact gctgccatat
     8581 ggcgaatttc gcgcagtaga cggtcgtccg actgatgtcc ctgcgtggta tctgaccgaa
     8641 gaaaacggtc atgatgtcgc gttgttggcc aacagctcgc gcaatcagtt ggttgtcgat
     8701 tatgaacacc agacgctcta caaagagaaa aacggacaac ctgcacctgc cgccggttgg
     8761 atgcgttggc tggagttcac gcctaaaggc atgtttgccg aagtggagtg gacggacaag
     8821 gcggctgcgg caattgccgc aaaagagtat cgctacatct ctgctgtgtt ttcctatgac
     8881 acaaagggat atgtaagcaa aatttttcac gcagcgctga caaatttccc cgcgttggac
     8941 ggtatggacg aggtgctggc ggcagcgtcg gcgcaaattt taaaaccgga aacggagcaa
     9001 aaccctatga aagagttgtt acagcaactg ttcggtctgc ctgatgcggg cgaagaagaa
     9061 ctgaaggcgg cattgtccgc gctcgtggaa gccaagccgg aaggtgcggc tttgtccgcc
     9121 gatgtattcg cgcagctggc ggaaaaagac agccgcattg cggctttgtc ggcgcaaaac
     9181 ggcacgcccg acctgacgcg atacgcgccg gtgtcgctgg tgcgcgacct gcaaaaccaa
     9241 gttgccgccc tgaccgccga gcgcgaagcc gacaaaggcg gcgaactgat taccgccgca
     9301 ctgtctgctg gcaagctgct gcccgcacaa aaagaatggg cggaaggcgt gctgaaacag
     9361 ccgggtggct ttgccttttt gagcggcttt atcgaaaacg cccagccggt cgctgcactg
     9421 gcaggctcgc aaacgggcgg caaagcaccc gacgaacgcg tcgccgcact gactgcggaa
     9481 gaggcagccg cagcaaaaat gctgggcatg tccggcgaag aatttgtaaa aatcaaagaa
     9541 agcgaaggta agtaatggac aaatcagcga ttttgaccgc aatcacggca gcattccgca
     9601 aagaatttca aacgggggcc tgcaaaaccg cctgaaccgt ttaaccgcca aataggtgca
     9661 gccggtagcg caggcagtgc gcctgttatc cacgtcatcg ctgttgtcgg tggcaacggc
     9721 attaatcgag gcgcatggcg aagagatgac cgcgcccgat ttgattgagg ttaaccgcgc
     9781 catgcgccgc cgtatgcagg ccgagattgc cgccttgcgg gcggtgcaga cggctgctgc
     9841 cgagtctggt gggctgacgg ccaacgccgt gtataccgag gcttaccaaa cggcagaatc
     9901 cctgcgcgcg gcggcaggca gtctgaatgc gttggttgcg gcggccatca accaaaagcc
     9961 gccgctgatt gtgcgccaag ccccaatcga cggtacgata caccaaatcg cccacgagtt
    10021 ttacggcgat atagcccgcg cagcagagct ggtgcgactc aatccccata tccaccaccc
    10081 cgcgtttatc aagcgcggca ctttggtcaa cagctatgca aaataattca tacggctatg
    10141 ccgtgtcggt gcgcgtgggc ggtaaagagc accgccactg ggagcgctac gacatcgaca
    10201 gcgacttttt aatccctgcc gacagcttcg attttgtcat cggcaggttg gggccggagg
    10261 cggccatacc cgatttaagc ggagagagct gcgaggtagt gatagacggg caaatcgtga
    10321 tgacgggcat catcggcagc cagcgccacg gcaaaagcaa gggcagccgc gagttgagct
    10381 tgagcgggcg tgatttggcc ggttttttgg tggattgctc cgcgccgcag ctcaatgtaa
    10441 agggcatgac ggtattggat gcagccaaaa agctggccgc gccgtggccg cagattaaag
    10501 cggtggtgct taaggccgaa aacaaccccg ctttggacaa aatcgacatc gagccgggcg
    10561 aaactgtatg gcaggcatta acccatattg ccaactcggt cgggctgcat ccgtggctgg
    10621 agccggacgg cacgttggtg gtgggcggtg cggattacag cagcccgccg gtggcgacat
    10681 tgtgttggag ccgcaccgac agccgctgca atatcgagcg catggacatt gagtgggata
    10741 ccgacaaccg cttttccgag gttacttttt tggcgcaatc gcacggccgc agcggcgaca
    10801 gcgccaaaca cgatttaaag tgggtgtaca aagacccgac gatgacgctg caccgcccta
    10861 aaacggtggt ggtgtccgat gccgacaatt tggccgcatt gcaaaagcag gctaaaaagc
    10921 agctggccga ctggcggctg gagggattta cactcacgat aaccgtgggc ggccataaaa
    10981 cccgcgacgg cgtattgtgg caacctggcc agcgtgtgca tgtgatcgac gacgagcacg
    11041 gtatcgatgc ggtgtttttt ctgatggggc ggcggtttat gctatcccgc atggatggca
    11101 cgcaaaccga gctgcggctc aaagaggacg gtatttggac acccgacgct taccccaaaa
    11161 aggccgaggc ggcgcgcaag cgcaaaggca aacgcaaagg cgtgagccat aagggcaaaa
    11221 aaggcggcaa aaaacaagca gaaacggcgg tgtttgaatg agtttgagta aattggcgaa
    11281 aaaaacggca caaactgcta aaaatatcgg cgaaaccctg cgcacggcct ttcggggaaa
    11341 aatcacgctg gtggtgtcgt ccgagccgat acagcgcgtg cagttgagcg gcttggccga
    11401 cgaaaccctg caagaccttg aacatttgca ggaatacggc tttgccagcc atccgcccga
    11461 cggcagcgaa gcggtagtga taccgctggg cggcaatact tcgcacggtg tgattgtgtg
    11521 cagccagcac ggcagctacc gcatcaaaaa ccttaagccc ggcgagacgg cgatttttaa
    11581 tcatgagggt gcaaaaatcg tgattaagca aggcaaaatc attgaggccg attgcgacgt
    11641 gtaccgggtt aactgcaaac aatacgaggt taatgcggcc acggatgcca aatttaacgc
    11701 tccgttggtg gagaccagtg cagtgttgac ggcgcaaggc caaatcaacg gcaacggcgg
    11761 catggccgtc gagggcggcg acggagccac ctttagcggc gatgttaacc aaacgggcgg
    11821 cagctttaac accgacggcg acgtggtggc cggcaatata tcgttgcgcc agcacccgca
    11881 taccgacagc atcggcggca aaaccttacc ggcggaaccg gcatagacaa gcagaccttt
    11941 ggcagccttc gggctgcttt ttttgtgcgt gtgggattga agcccgtgta ctccgtgagg
    12001 ccgtctgaaa acggcaaaat gccaacatgg acaaagagct aaaccccagc atcggcgact
    12061 ataccggccg caccgtcgat acgctgcaaa atgccgtgta tatccgcttg atgacaccgt
    12121 tgggcagctg gtgggcggat aaaacgctcg gctcgctgct gcatttgttg cagcgcgaaa
    12181 aagacctgca acgggtcagc ctgttggccg agcaatatgc cgatgaggca ctgcaaccga
    12241 ttgttaagag cgggcgtgcc gacaagatta ccgtgcgcgc agagcagccg cacgacggcc
    12301 gcctgatcct gcatatccgg gtggatacgg cggcgggcgg gtttgattac cgccacgaag
    12361 tgcccgtgat ttaaagaggt tttaaacgtg tttgaaacgc cgacatttga gcaaatccgc
    12421 gagcgtatcc tgcgcgatac caaaagcctg tggccggatg ccgatatcag ccccgacagc
    12481 gaccattatg tgcacgccag ccgtttggcc agctgcgccg aagggcaata tgcgcatcaa
    12541 agctggattg tgcggcagat tttccctgat accgccgacc gcgagtattt ggagcggcat
    12601 gcctccatgc gcggcttgcg ccgccgcaat cctaccacgg ccagcggcac gctgaccgta
    12661 agcggtattg cgcaatccat gctttcagac ggcctgcaag tgcgtatcgg ccagcgtttt
    12721 taccgcacta ccgcccgcgc cgttatcggc agcggcggca cggcggaaat accggcaatc
    12781 gccgacgagc tgggcgcggc cgccaatgtg cgcgacggcg aggcgcaact gatggccgcc
    12841 cccgccggtg tgtccaccga atgccgcctt accgtacaag gcggcaccga ccgagaaagc
    12901 gatgcctcac tgctggcgcg tctgttggaa atcatccgcc gaccgcccgc aggcggcaac
    12961 cgttacgact ataaaaactg ggcgttgagt gttgacggcg taaccagcgc atatgtttat
    13021 ccgctgcgcc gcggcttggg tacggtggat attgccatta cctccgccga cggtgtgccg
    13081 tcggaagaaa ctgtgcgccg cgtacaggct tatatcgacg agatgcgccc ggtaacggca
    13141 aaaaatgcgc tggtactcaa gccaaccgta acggcggtgc ctgttaccgt gcaagtcaag
    13201 ctcgacggca tcgacttgga cgatgccaag cgccgcatac ggacggctct aaaagaatat
    13261 ttcgacaccc tgatccccgg cgacggtctg actgtgtcgc aaatcgaggc ggctatcagc
    13321 aatgtggatg gtgtgatcga ccgccgtctg actgcgccga cggccaaccg tgccgccgat
    13381 acggttaacc gcatcgagtg gtttaaagcg ggcgcgatta atgtaacgga gatgccgtca
    13441 tgagctatca agacatcttg cggggtctgt tgccccccgt gtcgtatgcc cgcaatgccc
    13501 cgcgtgtgcg ggcgcaggca gaaatagacg gcgcagcgct ggatgcggtg gcggaatcgg
    13561 ctcaaagcgt tgccgatgcc gtcgacccga gcagcgccgg ccaaatgctg gccgattggg
    13621 agcgcgtatt aggtttggac ggtacgggca aaaaccgcca gcgccgtgtg ttggccgtca
    13681 tggccaagct aaacgaaaca ggcggcttga gtattcctta ttttgtgcgt ttggccgagg
    13741 cggcgggcta tcaaatccaa atcgacgaac cgcagccgtt ccgcgccggt gtaaaccgcg
    13801 ccggcgaccg tcttgcgccg caggaaatca tgtgggtgtg gcacgttaac gtgcgcggcg
    13861 gcaacaaccg cattacccga ttccgcgccg gtatctcggc ggcgggcgac aggctgaccg
    13921 attacagcga tgccgtgatc gagagcctgt tcaaccgcct caagcccgcc cacaccgcta
    13981 tccgatttac ctaccgataa ggactaaacc atgcacccca tcgaaacccc cgacaagacc
    14041 ttccacgacg gcgacggcgt gtccgaattg ggcaccatcc tgcccgcgtg gtggctcaac
    14101 caagtgcaat ccgagctgct ggccgtgctg actgcggccg gtatccagcc ggataagtcg
    14161 cagcctaatc aattactggc agcactaaat aggctggctg tagttaccac cggcgaccaa
    14221 accatcaccg gccaaaaaac ctttactgcc caaacccaat tccaaagcgg catccattta
    14281 tccgccaacc agacgaactg gaacggcggc cacaaagcct acatcggcgc ggctgccgac
    14341 aacgcccaca tcgtcttcgg cgacaacacc ctccgcctgc acggcgcaaa caaccgcatt
    14401 tcctacaaca accacgacat cttccacaaa gccaacaaac cgcgttttgc cgaagacatc
    14461 aaaggcaaac cgaacacact gtccggatac ggcatcggca atttcaaagt cgaaacattc
    14521 cggggcgatt tgaacaccct caaaacagac ggcatctatt ccctgccgac ggcggtcggc
    14581 agctccaacc tgcccgttga aaacaccgcc tgccatatcc aagtcatcgc cggaacgcaa
    14641 cccggctggt gcaggcagtt gggttatccc gcctacacgt ccgacgtgta cgaacgccac
    14701 caaacgagca gcgcaaacga caactggtcc gcatggaaaa aactcaattc ggacggcatc
    14761 cccgccggcg cgatcgtatc ctttcccaaa gccgtacaaa accccgcagg ctatctcaaa
    14821 gccaacggca cgacctttgc gcaaaacacc ttccccgacc tctaccgcgc cttgggcaac
    14881 agcaaccgcc tgcccgattt gagccgtacc gacatcggca tcaccgcgtg gtttccgtcc
    14941 gaccaaatcc cgaccggctg gctggcgttt gacgacatcc gcacgcgcgt aaccgaaacc
    15001 gcttatcccg agctgtaccg tctgctgacc ggaaaatacg gcagcatcca aaacgtcccg
    15061 caggcggaag accgctttat ccgcaacgca ggcaacagct tggcagtcgg aacgaagcag
    15121 gaagacgaaa tcaaacggca cgtccacaaa gtattttcac actggacaaa ccacacagac
    15181 acggcagccg tcggttacga agaccacaac gaaaggcaga gaagcgcgct cgtatcgact
    15241 tggacggacg gcgatttaaa cgacaacggc tttttaaccc cgcgctcgga cagcaaaatg
    15301 gcaacgggcg gcgacgaaaa ccgccccaaa gccctggttt taaaactgtg catcaaagcc
    15361 gccgacacct tgggcgaagc cgtgttttgg ataaagtccc acggcgaaac catcaacgca
    15421 ggcgcgctgg acgcgggcac gctggcgcaa ggtttgcaag acaaagccga ccgcgaccac
    15481 acccacaccg ccgcccaaat ccaagggctg gacgaaaaaa tcagcaccgc cgttgccgcg
    15541 caattcacac gccaaaccat cggcggcgtg gatattgtca gattccccga cggcacaatg
    15601 atacagaccg gcagttacag gtttacaaga agcggcggcc ccatcgaaaa cgaagtcgtc
    15661 ttccccgtcg cctttgccga cggcaacgtc aaatgcttcg tatccgaacg ccattcggga
    15721 cgcgttaccg gcgatcgaag gcaacacaac tggctgttta tccgcgcaaa aaaccacgcc
    15781 gccgccatta ttaccaactg gtacgaaggc agttgcgact ggatggccat cggcaaagcc
    15841 gcctcgggaa acgccgccag ctccccgata ggccccgaaa tacctgaaac caacgaagaa
    15901 ccgcaaagag agagtggaag aacatcaacc ggaccccgaa accgccgccg ccgagacggc
    15961 ttgctcgagg cactgcaaga ctagcgggct gtagagatgg ctgtagagac gggctgtaga
    16021 gatgggctgt agagatgggc ttcagcccgc cgatccaagc aatccgaccg aaaccggccg
    16081 cgccgccaat cccccgaaac ctatgccccg ccaatcctgc cactcttcgt cattcccgcc
    16141 gctttcgtca ttcccgcgaa agcgggaatc cagcccctaa acgcggcagg aatctatcgg
    16201 aaaaaacaga aacccccgcc accgtcattc ccgcgaaagc gggaatctag accccaaacg
    16261 cggcaggaat ctatcggaaa aaacagaaac ccccgccgcc gtcattcccg cgaaagcggg
    16321 aatccagacc ccgacgcaac aggaatctat cggaaaaaac agaaaccccc gccgccgtca
    16381 ttcccgcgca ggcgggaatc cagaccccaa acgcggcagg aatctatcgg aaacggctga
    16441 aaccgaacgg actggattcc cgcctgcgcg ggaatgacgg cggcaggggt ttcgggattc
    16501 ccgccttcgc gggaatgacg gaaagtggcg ggaataacga aaggcgggaa tgaccgcgca
    16561 aaaagccgct gcccccttcg gacggcaccg gcaacaaaaa accgcacggc cgaaaccgcg
    16621 cgggaaagga tagtcgggcg cgcccgataa gcagcggccg cccccgttat ttcaattggg
    16681 cgacatattg gcgcaaaacc tcgttgatgc gcgtctgcca gcccttgccg ccggcgcgga
    16741 atttttcgac cacatcggcg gacaggcgta tggtaacgag ttgtttcggg gttttgcctg
    16801 tgtttcgttt ttgcattacg cccttttctt ccaattgttt ttgatgggaa aaaagcacct
    16861 gtgccaagtc ttcgggcagg gcttcgacaa tggggcgggc aagcgcaaaa tcttcggcgg
    16921 caagttcccg cacttcgccg tcagcgtttg ttaaggattg acgttgcata ttttttaacc
    16981 tctcttttat tcgctttgcg aaaactgatg acacggatgc cgtcttttat cggcgtaaaa
    17041 cagacaatgt gcaggcgttg cgtatcgcct agataagcag cggcaacata acgcggttcg
    17101 gggtaatcaa agcagacatc gggcacaata acggccgttg tccagcgtat ttgcccgact
    17161 gattcaaagg gcaaattccg ctcttcgata ttgcgttgat ttttttcgga gtcaaattca
    17221 atcttcattg cagcttgcag cgtattttgt cgttacatta taagcggcaa aaaaccaaaa
    17281 tgtaaataca aaaaaggaaa ccccgaaatg accatctatt tcaaaaacgg cttttacgac
    17341 gacaccttgg gcagcatccc cgaaggcgcg gttgccgtcc gcgccgaaga atacgccgcc
    17401 cttttggcag gacaggcgca gggcgggcag attgccgcag attccgacgg ccgccccgtt
    17461 ttaaccccgc cgcgcccgtc cgaataccac gaatgggacg gcaagaaatg ggaaatcggc
    17521 gaagccgctg ccgccgcccg tttcgccgaa caaaaaaccg ccacggcatt ccgcctcgcg
    17581 gcaaaggcgg acgaactcaa aaacagcctc ttggcgggct atccccaagt ggaaatcgac
    17641 agcttttaca ggcaggaaaa agaagccctc gcgcggcagg cggacaacaa cgccccgacc
    17701 ccgatgctgg cgcaaatcgc cgccgcaagg ggcgtggaat tggacgtttt gattgaaaaa
    17761 gttgtcgaaa aatccgcccg cctggccgtt gccgccggcg cgattatcgg aaagcggcag
    17821 cagctcgaag acaaattgaa caccatcgaa accgcgccag gattggacgc gctggaaaag
    17881 gaaatcgaag aatggacgct aaacatcggc tgaaaaaata cgtttaccac ctgttggtag
    17941 ccatcgacca actgttcaac gccctaaccg gcggcgcggc ggacgaaacc ctctcaagcc
    18001 gcacctatcg cggcgcgcgg ctcgcccaaa agcccaaaac ccgctggaag gttttataca
    18061 ccctgatcaa cggcgtattt ttcgaccgcc aacactgccg gcaggcgtat atcagcgaac
    18121 tgaaaggcag gcagcacgac gcgcggttca accaaagccg cgccgccggg gaaaagggga
    18181 cgcgatgaac tacttcggca tggtagagtt tctgcgcctg atggcaatgg tgcggccgcc
    18241 atttgtcttc ttctccggca cccgcagcga actgcctact tatcttgacc tcgtggcaga
    18301 actgcgcctg accggatggg agcgttttgc aggcagccaa accttgactg tgagcagcac
    18361 atcaacagca attcaagcta cgacgaccac ctgatttata agttctgacc gcaagtagcg
    18421 tactactttt aaaggcataa gataatcccc gtttaacaga ccattaaacg gggataaatt
    18481 tgtgcaaaag ctaatacaat ttcctacgct tcggcggtgc aaaagctgcc gccaattcgt
    18541 gcaaaagctg ccgccgcctt acattccagt gcaatgccgt ctgaaacttc gctaatctcg
    18601 ggttgccgcg cgctgtgttg ttcttcggta ctcagcaaaa agccgtacag acgctccaat
    18661 cgggcgcggt aggcggtgaa tttgttgtag aacatccgga aaaaagatag cgcgttttgc
    18721 agtcgcgcaa aggcttggac ggtctgctgg atgtcgccga ttttgatttg tccggcaaac
    18781 aggcgcggcg cttgcaaaat aatggggaag agtttgatgc cgttggtgaa catatcgtta
    18841 aagccgctta agcagacgct ttgccgcgcg atgcgccaac ggttgcggat aatggcttta
    18901 aagcggtcgg aaagccggcc gtgttcgtgt tgttcgccgt tgtaaaacgc tacgctttcg
    18961 gcgtggtcgc gtacgcggat gagggaataa cggtagtcgc cgttgagttt ttcgttttca
    19021 tagttgtaac gaatcaaggg gttgcctatc cacatcgcga taaaggtcgc caaaatcaca
    19081 aaaataaaga cgagccaaac aatgccgcgc ggaatgtcga agccgaacac ggtcaggatg
    19141 ccagccaagc cccacaaaac aacggcaaat tccaaagagg taacgaccga attgaccatg
    19201 ccgcgtacaa attcgatggt cgaagcgatg aaatcctgcg cgtcctgttg gatacgttgg
    19261 tcgatgttgt ccggcgcgtg gcggcgcatt tgcaggcggt agtagttttt gtcggcaagc
    19321 cagcgcgtcg tcagcacttc attgagctgc tccgaccatt taatcgccaa gccttgatcg
    19381 aggaagtcgt tgacgacatt gttaaacgcc cgtatcagca ccacgcccgc gttcatcgcg
    19441 gcaaacatcc aaaacgcgga ggcgttcaaa tcctgcatcg agtcgtaaag tcctttggac
    19501 ataaaggtac tcaacacatt cagccgtact tcggtcaaca gcagcgtaac cattaccgta
    19561 atcagcagca agactttgac cgtgcttttc ggtgtcaggc aaagccaaag cggtgtggaa
    19621 taaagctcgg tttgccattt ctgcatggga aatttcttac ggtatcaatg ccgtctgaaa
    19681 aagacgggta cagttgattt tttgatgaag tttggggaag ttttgccggt cagggtacat
    19741 tgcgtg
//
LOCUS       DAXHPG010000022        19539 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXHPG010000022 DAXHPG010000000
VERSION     DAXHPG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19539
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.022"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            47..1225
                     /locus_tag="ACFM6F_000570"
     CDS             47..1225
                     /locus_tag="ACFM6F_000570"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC6164037.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASIASV
                     KYCVDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMEKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(1292..1540)
                     /locus_tag="ACFM6F_000571"
     CDS             complement(1292..1540)
                     /locus_tag="ACFM6F_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6164038.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(1631..2548)
                     /gene="ftsX"
                     /locus_tag="ACFM6F_000572"
     CDS             complement(1631..2548)
                     /gene="ftsX"
                     /locus_tag="ACFM6F_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC6164039.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITIYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(2545..3195)
                     /gene="ftsE"
                     /locus_tag="ACFM6F_000573"
     CDS             complement(2545..3195)
                     /gene="ftsE"
                     /locus_tag="ACFM6F_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC6164040.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(3192..3383)
                     /locus_tag="ACFM6F_000574"
     CDS             complement(3192..3383)
                     /locus_tag="ACFM6F_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164041.1"
                     /translation="MPSESASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            complement(3391..3873)
                     /locus_tag="ACFM6F_000575"
     CDS             complement(3391..3873)
                     /locus_tag="ACFM6F_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC6164042.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            3997..4350
                     /gene="arsC"
                     /locus_tag="ACFM6F_000576"
     CDS             3997..4350
                     /gene="arsC"
                     /locus_tag="ACFM6F_000576"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC6164043.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPNLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            4347..4991
                     /locus_tag="ACFM6F_000577"
     CDS             4347..4991
                     /locus_tag="ACFM6F_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC6164044.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPFLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGRDFFN"
     gene            5195..6589
                     /gene="gltX"
                     /locus_tag="ACFM6F_000578"
     CDS             5195..6589
                     /gene="gltX"
                     /locus_tag="ACFM6F_000578"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC6164045.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFERYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNEKLAELVKPRLA
                     LRDIHETEKPALEDVLAQVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            6610..7005
                     /locus_tag="ACFM6F_000579"
     CDS             6610..7005
                     /locus_tag="ACFM6F_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164046.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDYAWNAVYGAGGTKASGN"
     gene            7009..7500
                     /locus_tag="ACFM6F_000580"
     CDS             7009..7500
                     /locus_tag="ACFM6F_000580"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6164047.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(7623..10217)
                     /gene="mutS"
                     /locus_tag="ACFM6F_000581"
     CDS             complement(7623..10217)
                     /gene="mutS"
                     /locus_tag="ACFM6F_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC6164048.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPAIHIENGRHPVVEQQVRHFTANHTNLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPRKALSELYRL
                     KDLCKSVS"
     gene            10659..11663
                     /gene="gap"
                     /locus_tag="ACFM6F_000582"
     CDS             10659..11663
                     /gene="gap"
                     /locus_tag="ACFM6F_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6164049.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(11733..11852)
                     /locus_tag="ACFM6F_000583"
                     /pseudo
     CDS             complement(11733..11852)
                     /locus_tag="ACFM6F_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11921..12601)
                     /gene="tsaA"
                     /locus_tag="ACFM6F_000584"
     CDS             complement(11921..12601)
                     /gene="tsaA"
                     /locus_tag="ACFM6F_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC6164050.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEHFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            12716..13351
                     /locus_tag="ACFM6F_000585"
     CDS             12716..13351
                     /locus_tag="ACFM6F_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC6164051.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYDY
                     CVWKGIDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            13388..14356
                     /gene="waaC"
                     /locus_tag="ACFM6F_000586"
     CDS             13388..14356
                     /gene="waaC"
                     /locus_tag="ACFM6F_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC6164052.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            14583..15332
                     /locus_tag="ACFM6F_000587"
     CDS             14583..15332
                     /locus_tag="ACFM6F_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC6164053.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDAKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLATDILPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            15343..16278
                     /locus_tag="ACFM6F_000588"
     CDS             15343..16278
                     /locus_tag="ACFM6F_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC6164054.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGSGASAVV
                     ESAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQANAEKLVLTFRATAFDAAAVQGGN
                     AEVINIETTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            16341..16952
                     /locus_tag="ACFM6F_000589"
     CDS             16341..16952
                     /locus_tag="ACFM6F_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC6164055.1"
                     /translation="MITTYKTITSPTQAGFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGMQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVSGLIHAYKEATAQALAIAEVIEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILEQDLQLDCRLTVSLSLANYEVCVAAWKNTRQIEVNTEKPFE"
     gene            complement(17004..17321)
                     /locus_tag="ACFM6F_000590"
     CDS             complement(17004..17321)
                     /locus_tag="ACFM6F_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC6164056.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            17479..18750
                     /gene="purD"
                     /locus_tag="ACFM6F_000591"
     CDS             17479..18750
                     /gene="purD"
                     /locus_tag="ACFM6F_000591"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC6164057.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGKLDSVAAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            18784..19386
                     /locus_tag="ACFM6F_000592"
     CDS             18784..19386
                     /locus_tag="ACFM6F_000592"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6164058.1"
                     /translation="MTDMLQTAFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         4719 a         4443 c         5467 g         4910 t
ORIGIN      
        1 cattaattgt ttctgtatcg caaacactga ttgaaaagga ctgattatgg catttttaaa
       61 actgaccgaa caaaacgtgc agggcaaaac cgtcctcatc cgcgccgata tgaacgtgcc
      121 gttcaaagac ggcaaaatca gcgacgacac ccgtatccgc gcctcgattg cgtccgttaa
      181 atactgcgtg gacaacggcg cgtccgtcat cgtgatgacc cacttgggcc gcccgaccga
      241 aggcgagttt caccccgaag acgacgttgc gcccgttgcc gcgcatttgg gcggcctgtt
      301 gggcaaaaac gtgaaagtat tgaacgactg gcgcgaaaac aaaccggctt tgaacgcggg
      361 cgatgtcgtg atgctgcaaa acgtgcgtat caacaaaggc gagaagaaaa acgatttgga
      421 actgggcaaa gcctacgctt ctttgtgcga cgtgttcgtc aatgacgcgt tcggcaccgc
      481 gcaccgcgcc caagcctcga ccgaagccgt cgcccaagcc gctcccgttg cctgcgccgg
      541 cgtattgatg gcgggcgaac tcgacgcttt gggcaaagcc ttgaaacagc ccgcgcgccc
      601 gatggttgcc atcgttgccg gcagcaaagt gtccaccaag ctgaccatcc tcgaatcgct
      661 ggcggacaaa gtcgaccaac tcatcgtcgg cggcggtatc gccaacacct tcctgttggc
      721 ggaaggtaaa gccatcggca aatctttggc ggaacacgat ttggtggaag aatccaaaaa
      781 aatcatggag aaaatggcgg ccaaaggcgg ttctgtgccg ctgccgaccg atgtcgtcgt
      841 tgccaaagcc tttgctgccg atgccgaagc ggtcgtgaaa gacattgccg acgttgccga
      901 agatgagatg attttggaca tcggcccgaa atccgccgcc gcgcttgcgg agttgctcaa
      961 agccgccggc acggtcgtat ggaacgggcc ggtcggcgtg tttgagtttg accagttcgc
     1021 aggcggtacg aaagcccttg ccgaagccat tgcccaaagc aaagcattct cgattgcagg
     1081 cggtggcgac acgctggcgg cgattgctaa attcggcgtt accgaccaaa tcggctatat
     1141 ttctaccggc ggaggcgcgt tccttgagtt cttggaaggc aaagagctgc ctgccgtagc
     1201 tgctttggaa aaacgcggcg cgtaagcggt ttgatataaa aaatgccgtc tgaaacgttg
     1261 tgctgtttct gacggcattt ttgtgtggcg gttattgtgc tttggctgcc cattccgccg
     1321 gagtggcggc aaccgaaatg gaaagtgcgt gcagttcgtt actttccagt tgggctttga
     1381 gtccgtcttt aatcaggcgg tggcgcgcga ggcgtgcctt gccttcaaat tctgatgaaa
     1441 cgatgacggc gaaaaaatgg tgtccgtcgc cttctacttc gatatgttcg cattttgcca
     1501 cgccttcaat catggctttt acttgttccg aggtcagcat ggtaggttcc ttgttggaaa
     1561 aatgcgccat tatactggca aattgaaatg ccgtctgaaa acgggttcgg acggcatttt
     1621 tgacggtgtt ttattttttg gctttgaagc cgagcaggtg ctgggtggtg gcaagccacg
     1681 cgccgaatac gcccaacgcg atgacgaagc cgaacaccag tgcgatttct cccgggtaga
     1741 agaaacgcca gccgatatta agtccgtagg gtttgaagat ggcatcgacc aaggggcgca
     1801 cggcagagag cagccaaccg caaagcccca agctgacggc ggcggaaagg atgctctgcc
     1861 acatggcttg ataaaggaat gggcggcgga taaacgacgc gggcgcgccc aagagtttgg
     1921 tgatttcgat ttcttctttg cggctgagga tttgcagtcg gatggtgttg tgcgcgacaa
     1981 ggacgaacgc catccccagc gtcagggaaa gaaaccataa gattttacgg atgaattcgt
     2041 tgatttggta cagtgtctgt acccattccg tatccatgtt ggcggattcg accataggca
     2101 gtttggtaat gtcccggtag attgcctgca tttgggcggg cgtggttgcc gggtcggggg
     2161 taacgataaa gacatccggc agggggttac cgtcaagcat ggaaatcaga ttttggtcga
     2221 gattggactg taattcttcc agaccgtctt ctttgcctat gaagcggatg ttgtcgagcc
     2281 gtttgtcgcg cgccagcagg ctgcggacgg tatcgctgtc gctttgtgcg gcggcggttt
     2341 ccatatagat tgtgatttgc ggcgactcat tgagtttgcc caacacgctt tgcccgcttt
     2401 ggatgcccaa atacataaac agcggcaggg tcatggcgac ggcgagcatt atgagggtaa
     2461 gcagtgtgcc gaaaggttgg cgcagaagct gtttgagcgc ggtgcgcgcg gattcgacgt
     2521 gcagcgagag gtagtggatg atgctcatgc gagtcgtcct ttcgagaggc gcaggatgcg
     2581 gtgtccgtag tccgccatca gggtttcgtc atgtgcggcg acgatgacgg ttgttcccgc
     2641 ttcgtggaag gttttgaaca attccataat atcgagcgcg taggcgcggt cgaggttggc
     2701 ggagggttcg tcggcaatca gcaggccggg ctggtgaacg acggcgcggg cgatgcacag
     2761 gcgttgttgt tcaccgccgg agagggttac gggatcgtcc aattctcgtc ctttcaggcc
     2821 gactttttcg atggcgatgc gggcacgctc ttcggctttg cgcggcggat agccgataat
     2881 ccgaagcggc aggatgacgt tttgcaggac gttgcggtcg tagaggattt tgtggtcttg
     2941 gaacacgatg ccgatgtgtt ggcgcataaa gccgatttgg ttgtcggaca atgtgccgag
     3001 gtcctgcccg ttaaacagga ttttgcccct gctcggcttg gtgatgcccg aaatcagctt
     3061 gaggacggtg gatttgcccg aaccggagtg tcccgcgata aatatcattt cgcctttgtt
     3121 gatttggaag ctgacgtttt tcagggcttc aaaaccgccg ggataggttt tggaaacttg
     3181 ttcgaaacgg atcataggag gtcctgtaag gaatgggcgg catatcgttt cgggcggttg
     3241 cgtccgcagg gatatgccgt ctgaacaata ggtaagacag tgccgattat aaagattccg
     3301 cctgttttta gatagtggat aagtttggcg gataccgaag gtcgggcggg aaaaatgccg
     3361 tctgaagcgg attcagacgg catccggcgt ttaacggcat tttgaatggg cgagtttgac
     3421 ggcttcggtc aggctttttt cgttcacttc cccggtaatg gtttgtttgt atccgcattt
     3481 cggggcttcg acgacggtaa agggcagtac gccgacattg tttccgtagg atttcataaa
     3541 gtttcggctg ttcgccccgg tgtaacgcca aatcgggtag gaaacagggg tttgcttgag
     3601 gaagttgccg atattgtcgg atgtgtcgag cgcgatgccg accatatcga cactgccttt
     3661 tttctgcgct ttgtaccatt tggacatggc aggcatctct tttcggcacg ggccgcacca
     3721 ggttgcccaa aggttgacga tgcgtacggg ggctttgagt gattgcaggc tttgcggggt
     3781 gttgtctttc catccggcca gttcgtctgc cgaggcggta tgtgcggcaa atgtggcggc
     3841 aagggtgatt gcggcaagat tcaagtattt catcgttttg tcctttgtat gtcgattcgg
     3901 gtattgtaaa ccgtttggta cggttgtagg cgggatattg agtaaaaccc tgtgtaagcc
     3961 gataataagg ggattgtcga gaagtaagga aatgtcatgt ctgaaatcaa aattttccac
     4021 aacccccgtt gcagcaaatc gcgtgccgcc ttgtccctat tggaagaacg cggcattgct
     4081 gccgaagtgg tcaaatattt ggatacgccg cccgacttgt ccgaattgaa ggatattttc
     4141 aacaaattgg gcttggcatc ggcgcgcggg atgatgcgcg tgaaagatga tttgtataag
     4201 gaattgggtt tggacaaccc caatttggac aatgacgcgc tgctgcgtgc catcgccgat
     4261 catcccgccc tgttggagcg tccgattgtt ttggcgaacg gtaaggcggc tgtcggcagg
     4321 ccgttggaaa atatcgaagc ggtattgtga acaaacggct gttttgcagc cgcaacggct
     4381 tgcgctatta cctgttgggc ggtttctgtt tgagtgtttt tccgtttttg ctggtgtttg
     4441 cctcatccgt ttgggcggtt tatcggacgg gcgggcaggt tttgccgccg tatgtccggg
     4501 cggatgccgc gctggtgttg ggcgccgccg cgtgggacaa acgcccttct ccggtttttc
     4561 gggaacgcat caaccacgcc atcgcgcttt atcagagccg cagggtaggc aaaatcgtgt
     4621 ttacgggagg gcgaaccaaa aaaggctata tgaccgaagc cgaggtcggg cggcggtacg
     4681 cgctcaaaca gggggttccg gcgcgcaaca tcctgtttga aaacacttcg cgcaacactt
     4741 atgaaaacct gaacaacatc cgtccggttt tgcgtgccaa cggtattgcc agcgtggtga
     4801 ttgtcagcga tccctaccat ttggcacggg cggcggaaat ggcggaggat ttgggtgtcc
     4861 gcgtttatat gtctgccacg ccgaccacgc ggtttgatgc gggaaataag aaaaaaatct
     4921 ttatgctgca agagggatat gcgttatccc tctaccgttt ggaaaaatgg gggcgggatt
     4981 ttttcaatta gctgtttcat tgaacttttt tttggacgca atcccgaaga aatccggtcg
     5041 gcggcgcgtg atacactgtt ccgcgtgccg aagcattgaa aacaaggaaa atgccgtctg
     5101 aaacttatcc gcaggcaggg ttcggacggc atttttaatg cccaagcacg gtaaaaacag
     5161 taaaataagc cgttttaaag attctggagc catcatgacc gtcaaaaccc gtttcgcccc
     5221 cagccccacc ggctacctgc acattggcgg cgttcgcacc gccttgtttt catgggcgtt
     5281 tgcccgccat cacaaaggcg agttcctatt gcgtatcgaa gacaccgact tggcgcgttc
     5341 caccgccgaa tccgtcaaca tcatcctcga cggcatgaaa tgggtcggtc tcgattacga
     5401 taacgccgac aacgtggtgt accaaacccg ccgcttcgaa cgctataaag aagtcatcgc
     5461 cgaactcttg gcaaaaggcg atgcctacta ctgctattgc agcaaagaag agctggaagc
     5521 gatgcgcgag aaagccgaaa aagaaggcac ggcgacttac gaccgccgct ggcggccgga
     5581 agcaggcaaa accctgcccg aaatccctgc cggcgtgcaa cccgtcgtcc gcttcaaaac
     5641 gcctttggat ggcgtaacca agtggacgga cctggtcaaa ggcgaaattt ccatccccaa
     5701 cgaagccctc gacgacctga ttattgcacg cgccgacggc acaccgacct acaacttctg
     5761 cgttgtcgta gacgactacg acatgggcgt tacccacgtt atccgcggcg acgaccatgt
     5821 gaacaacacc ccgaaacaaa tcaacatctt aaaagccatc ggcgcaaacc tgcccgaata
     5881 cggtcatctg cccatgattc ttaacgaaca aggcaaaaaa atctccaaac gcagcggcga
     5941 tactgttgcc attaccgatt tcggcgcgat gggtatcctg cccgaagcca tgctcaacta
     6001 tctggcgcgc ttgggctggg cgcacggcga cgacgagttc tttacgatgg aacaattcat
     6061 cgaatggttt gatttgaaag acgtttcccc gtccccaagc cgtatggatt tgaaaaagct
     6121 ctattggatc aacggcgaac acatcaaaat cacgcccaac gagaaactcg ccgaactcgt
     6181 caaaccgcgt ctcgctctgc gcgatatcca tgaaaccgaa aaacctgcgt tggaagacgt
     6241 gttggcacag gttaaagacc gcgcccaaga tttgaacgcc ttggcagacg aatgtctgta
     6301 tttctacaaa aaacaagtcc ctgccgaagc cgatgttgcc aaacactggg acgacgaagc
     6361 cgccgctcgt atgctgcgct ttgccgaacg cctcgagggt ttggaagact ggaatgccga
     6421 agccatccac gacctcttca aacctttctg cgacgaagaa ggcatcaaaa tgggtaaact
     6481 cggtatgccc ctgcgcctcg ccgtctgcgg tacggcgaaa accccaagcg tcgatgctgt
     6541 gttggcatta atcggcaaag aagaagtgtt gaaacgcatc cgcgcttaag ttatccacag
     6601 gcgtgggcga tgccgtctga tgtcggaatg cggcttcaga cagcattcaa acggaagttg
     6661 aacatgaaaa aaatattgta ttttctgatg tttgtttttt ctacaagcgt atgggcgggg
     6721 gatgcggaag acaatctgct cagcatccaa tccggctatc gtgccttact gcaaaaacaa
     6781 aacaatctgg acggaaaaat catcgggatg cagtcggatt tggaagatgc gcgccggcgt
     6841 ttgcagacgg ctcaggcaga catcgcccgt ttggaagcgg aaattcctgc agcaatggcg
     6901 caaaaagccc ggcaggctga agagctgagg caaatcggag tgcgtttgga ctatgcttgg
     6961 aatgcggttt acggcgcagg gggaacgaaa gcgtcgggga attgaccgat gaattccctc
     7021 tttgtggaca atactgtttt cattacacgg ctgaaagccg ggcatatcgg caggttggtt
     7081 caggcgttgt ttgaggagtg gcacggattt gaaccgtggt cttctgtgga taagattcat
     7141 gcctattacg gcaggtgttt gaaggatgac gaactgccgc tggcttttgc ggctgtggat
     7201 gattccggaa ccctgttggg ttcggctacg gtcaagcggc atgatatgga aagttttcca
     7261 cagtatgaat attggttggg tgatgtcttt gttttacctg aatatcgcgg aaaaggcatt
     7321 ggcaggaggc tggtcgccca ctgcataggc gcagcgcgtt cgctggggat aaagttcttg
     7381 tatctttata cgcctgatgt gcaaatattt tatgaatcat tcggctgggc ggttgtcggg
     7441 cgacatttcc ataacggtga atgggttacg gttatgcgtt tggatatgga taaggtttaa
     7501 gccgaatgga caaatgccgt ctgagctgtt cggacggcat ttttatgttt ggaaacttct
     7561 tgtcagataa tgtttatcca cagatttttc catatggttt gatgctgttc cgccaacgga
     7621 aattaagata cggatttgca caaatctttc agacggtaca gttctgacaa tgctttgcgc
     7681 ggggtcaggc tgtcgggatc gagtttttcc aaggctgctg ccaatatacc ttcaaaatgt
     7741 ttttcctctg ctttatccac aaagttgccc acattcggtt catctccttt ttcagacggc
     7801 atggtactga aaatatccag ttggggacgg ttcgcggcgg cttggttttc cagttcgttc
     7861 aaatgctttt gggcggattt caatgcgcgt acaggcaggc cggcgagttt ggcgacggca
     7921 atgccgtagc ttttaccggc gggacccggt tggatttggt gcaggaaaac gatgtcctgt
     7981 ccctgttcga gcgcggaaag gtgcatattg acggcggcgg cgtgggcttc gggcaggtag
     8041 gtcagctcga aatagtgggt ggcaaacagg ctgaaggatt tgtttttttg cagcaggtgt
     8101 tcggcaacgg cgtgcgcgag ggcgaggccg tcgaaagtgg aagtgccacg tccgacttcg
     8161 tccattaaaa caaggctttg ttcggtggcg tgatgcagga tgtaggcggt ttcgctcatt
     8221 tcgaccatga aagtggagcg gttggaggcg aggtcgtccg atgcgccgat gcgggtgaag
     8281 atttgatcga tgggcccgat tgtggcggca tcggcaggca caaaacagcc ggtgtgtgcc
     8341 aataaaacaa tcagcgcgac ttggcgcatg taggtggatt tgccgcccat attggggccg
     8401 gtgagcagca tgaggcggtg tttgtggtca aggttggtgt ggttggcggt gaagtggcgt
     8461 acctgctgtt cgacaacggg atggcggccg ttttcgatgt ggatagccgg atagtcggca
     8521 aactcggggc ggacgaagtt ccgctctttt gccaaggctg aaaatgtgga caatacgtcc
     8581 agcgcggcgg cggctttggc ggctttttga agctgcggca atgccgtctg aaggtttttc
     8641 aatacgccgt caaagagttg tttttctaag gcgagggctt gctcttgagc agtcagcact
     8701 ttgtcttcaa aggctttcaa ctccggcgtg atgaagcgtt cggcgttttt gagggtttgc
     8761 cggcgttggt agtcggcagg tgcttgttcg gcttgggttt tggacaattc aatgtaaaag
     8821 ccgtgaacgc ggttgaactc gactttgagt gtggacaaac cggtacgttc gcgttccttg
     8881 gcttccaaat ccagcaaaaa ttcgtcgcca tggttttgaa tgcggcgcaa ttcgtccagt
     8941 tcgggatgaa aaccgtggtt gatgacattg ccgtctttca gccagacgga aggttcgggc
     9001 aaaatggctt ggcggagctg ttcggctgtg gataggtttt ccgggaaaac ggctttgagg
     9061 gtttccaaca ggctgctgcc gtttgcggac aagtcgattt gtgccaactc gaacagactg
     9121 tcgcgtaaag atgccaaatc gcgcgggcgg gcgttgccga cggcgatgcg ggcagcaatg
     9181 cgttcaatgt ccgcaatgtt tttcagacgg cattggaggg gtttgtattg gctttccagc
     9241 gcggcaacgg cttcttggcg cgctcggatg tgggcgcggt tgcgtaaagg gtggtgcagc
     9301 cagagagcca agaggcggct gcccatatgg gtagcgcaaa ggtcgagcgt ggacatcagg
     9361 gtcggcgatt ttttgccgga gagggtttgc gtgatttcga gattgcggcg cgtggcagca
     9421 tccataccga tatattggct gtcggtttcg agcgacaggc cgtccaaatg ttgcggcatc
     9481 aggttttgcg tcagacggat atagttcaac agtgcacctg ccgcgccaat cgcaacggcg
     9541 tgttctttgc cgtccaaacc gaagccgcgc aaatcctggc agccgaaata ttccgtcagc
     9601 agtttttccc ccgcgtcggc ggcaaactgc cacgcgttca ggcgcgtaac acccgatgcc
     9661 gtctgaagtt gcggtgcgtt tttactgtcc ggcaacagaa tttccgccgc ctgcaggcgc
     9721 gccagttcgt cgtccaattt atccacagtt gtcagcttgg ttttgaattc gccgctttgc
     9781 agcgatgccc acgccaaacc gatgtatttt ttgtcggggg acacggcaac gatacggttg
     9841 gtttccttgt cttccagcaa tgcggaatcg gtcagcgtgc cgggcgttac gatgcgcacg
     9901 actttgcgct ccacaggccc tttgcccgcg ccgacttcgc cgacctgttc gcaaatcgcc
     9961 acgcttttgc ccaacttgac taggcgcgcc agatattgtt cggcggcgtg aaacggcacg
    10021 cctgccattt tgaccggctc gccatccacc tgtccgcgcg tggtcagggt aatatccaaa
    10081 agttttgccg cttctaccgc atcgtcgaaa aacatctcgt aaaaatcgcc catacggtaa
    10141 aacaccagtt tgtcggtatg ttgcgctttg atgccgaggt attgctgcat cattggggaa
    10201 acggcggatt tggacatggg gctgccttat cattaggaaa acggtattgt agcaaaaggt
    10261 tttgtcttcg acggaatgag actgaccgta agcctgtcaa taaggttgtg gagacaacta
    10321 ggaaaacagc aaagttatcc acagaaatat tattaagtca aaaaacatcc acgcttatct
    10381 gtcttataca gcaagtctta aactcaaact tatttgacaa aaagataatg aaaatattgg
    10441 atatttgcta cttacgcaca agcaggttgg caatcatcaa aatcatcatt aaaatttaaa
    10501 ttcaaatatt gatggtaaag gttttggaaa tctgtgcaaa cagcaaaatt catctatatc
    10561 aaataagcgt caagtagtta gatgtaatag tggaatgtaa ctttatttca gaatacaatg
    10621 caggcatcgg actttatttc acaacctgga gacaaaatat gagcattaaa gtagcgatta
    10681 acggtttcgg ccgcatcggc cgcctcgcat tgcgtcaaat tgaaaaagcc cacgacatcg
    10741 aagtcgtcgc cgtcaacgac ctgacccctg ccgaaatgct gctgcacctt ttcaaatacg
    10801 acagcacgca aggccgtttc caaggtactg ccgaattaaa agacgatgcc atcgtggtaa
    10861 acggcaaaga aatcaaagtt tttgccaatc cgaatcccga agaattgcct tggggcgagt
    10921 tgggtgtgga tgtcgtcctc gaatgtaccg gcttctttac caataaaacc aaagccgaag
    10981 cacacatcag ggccggcgcg cgcaaagtcg tgatttccgc acccggcggc aatgatgtga
    11041 aaaccgtcgt atatggcgta aaccaagaca ttttggacgg cagcgaaacc gttatctccg
    11101 ctgcttcctg caccaccaac tgccttgccc cgatggcggc agtcctgcaa aaggaattcg
    11161 gcgtagtcga aggcctgatg accaccatcc acgcctacac cggcgaccaa aacacccttg
    11221 acgcgccgca ccgcaaaggc gatttgcgcc gcgcccgcgc cgccgcgctc aacatcgtgc
    11281 ccaacagcac cggtgccgcc aaggccatcg gtctggttat ccctgaattg aacggcaaac
    11341 tcgacggctc cgcccaacgc gtccctgttg ccaccggctc gctgaccgaa ttggtttccg
    11401 tcctcgaacg ccctgtaacc aaagaagaaa tcaacgccgc gatgaaagcc gccgccagcg
    11461 aatcttacgg ctacaacgaa gatcaaattg tttcttccga cgttgtcggc atcgaatacg
    11521 gttcgctctt cgatgcgacc caaacccgcg tgatgaccgt gggcggcaaa caattggtga
    11581 aaactgttgc ctggtacgac aacgaaatgt cctacacctg ccaactcgtc cgcactttgg
    11641 aatactttgc aggcaaaatc taaggcttga acaaacctgt ggataagtaa atgccgtctg
    11701 aacacattgc gttcagacgg cattgtttat ctgtatagtg gattaaattt aaaccagtac
    11761 ggcgttacct cgccttgccg tactatttgt actgtctgcg gcttcgttgc cttgtcctga
    11821 tttaaattta atccgctata acttgatatt gcggcttatg gcgaacaaac aacagccgtt
    11881 tgttcaactg ctatgcggca aaaccggatt tttgcccgat ttaaagcggg gttggggaaa
    11941 gatcaataac ggttgcacgg ttttcctcga tttgaaatct gacttcgtaa tctgcaatat
    12001 tcatcacata aatccgttcg ggaatattct gataggcggg gcgcggatct tgggcaatgc
    12061 tttggctgat aaggtttttg gtgtttgcag ataaattttc cgcgccgatg ttttcctgcc
    12121 aaacgacttc caactctacg ggtttgccgc tgacgaaacc agatgcggca tcgggtttgg
    12181 attcgacaaa ggggatataa ggcttgatgt ccacaatcgg cgtgccgtcc agcaggtctg
    12241 cgccgctgca atagaggcgg acgggtttgc cggtttcgat gcgttcgagt ttcaggagcg
    12301 agagtccgag atggttgggg cggtgggggc tgcgcgtggc gaatacgccc attttttgtt
    12361 tgccgccgag ccgtggcggg cgcaccattt gtgcccagcc ttcatccaat acgccgtgaa
    12421 aaataaaact tatccacaca tagtcgaagt gttccagtcc gcgtacgctg tctgcggtaa
    12481 attcgggatt cagctcgatg catacttctg cggcagagac caaaccgggc tggcgggcga
    12541 tgccgaattt ctgtttgtag ggcgatcggg cggtggcgat agggacgatg gtgtaagtca
    12601 tgaggattgt ggataagttt ttcggaaaaa ccttatctta tcacagcctg ttttaccgct
    12661 tttgggcagt aatttgttat aatacagggc attttaattg cgttgaaatg atgagatgat
    12721 tgtttccatt gatgttgatg cacaaaaaac ttttacgcct ttgtgtccta aagagctgcc
    12781 tgtaaacgag gggcatttga ttgtcgaaga attgaatgcc caagccgctt tggcggattt
    12841 gcgcgtgatg acgaaagatg cgcatcatgc ggcagcaaaa tggcttgtgg ataaccctgt
    12901 tgatatgttg aagccgacag gtttgcccga tgcggatttg acttgggtgg ctcatgcgat
    12961 ggttggtacg cgcggctatg aattgttgga cgggctgcct tgtgccaaag aatacgatta
    13021 ttgcgtttgg aaaggtattg atcctgaatt gcatccttac ggcgcgtgtt ttcacgatat
    13081 tgaggaaaaa ctaagcacag ggctgattga atggctgcgt tgtcaaaata cggatacggt
    13141 tattatcggt gggttagcta cggattattg tgttaaaaca acggttttgc agttactcaa
    13201 aggcggtcgt tggcaggttg tcgtcaatga agcggcttgt cggggtattg cgccggaaac
    13261 catcgaaaca gcatggcagg aaatgcattc tgccggggca atcatcttaa aaaacgctga
    13321 tgaaataaat aaatatatta ataatcaata agttaaaatt gtttcacgtg aaaccccctt
    13381 cctaaatatg aaaattttgc ttgtccgctt gtctagtatg ggcgatttaa ttcacacttt
    13441 gcccgcaatc gaagatttgg cgcgacaatg tcctgatgta gaactgcatt ggctatgtga
    13501 ggctggattt gcagatattg cgcgcctgca tccgtttgta aaaaaaatcc atgtgatgaa
    13561 atggcggcaa tggcgcaaac atctctttcg ggctgaaact tggcgggaaa tgggtcatct
    13621 gaaacagact ttgcggcagg aagtatttga tttcgtattg gacagtcaag gtctgattaa
    13681 aagcgcgtgt ttcgctaaaa tggcgaaatc ccctatttat ggtttggata aaaacagtgc
    13741 gcgcgaggga tgggctgctt tggcgtatgt aaaaacatac gctgtaccga agggaaaaaa
    13801 tgccgtttgg cgcaaccgtg aactgtttgc ccaagtattt gggtatgtaa tgccggaaac
    13861 gcaggtattt ggcttgactg ttcctgaagc aggccgtctg aaaaatttag cgcagccgta
    13921 ttatgcggct ttgcatgcga ccagtcggga cagtaagtta tggcctgtgg aaaactggcg
    13981 gtcgttgctg caaaagttga atgaagaaca gcaatgcaat atttacctgc cttggggaaa
    14041 tgaagatgaa aaaacgcgtg ccaaacaaat tgcagacgga ctgccgtttg ctattgtgtg
    14101 cgacaaaata aatttattgc aggcagcgta tctgctgaaa tacgcggtcg gaattgtcgg
    14161 cgtggatacc ggtttgctgc atttggcaaa tgccttggaa aaacctgtgg tcggcattta
    14221 taccgatacc gatccgatta aaacaggcgt tcaggtttcg ccaattgcaa aaaatttggg
    14281 caatatcggg cagattccga ccgcagattt ggtttatcaa gcgctgatgg attgtgttgc
    14341 agcagacaaa ggctaaaggg ttgtctgaaa ctgatatggc ggcatgatat tttccaattt
    14401 attaaataca ggaatgtgta tccgctagtc tgagtaaatg ctttaaaaat ggtataatcg
    14461 gcaagcatta tccgaaagat ttttgaatga attcgttttt aaggaaaacg gatttggttt
    14521 tatagggaag aaggtttggt ttcgctggat ggtgaaagca gcgttctttt taggagggtt
    14581 caatgaaagc actggtcgca gtaaagcgcg tagtggacta caacgtcaaa gttcgtgtaa
    14641 aagccgatgg ttcggatgtg gatatcggca atgtcaaaat gtcgatgaat ccgtttgacg
    14701 aaatcgctgt ggaagaagcc gtccgtttga aagaagccgg aaaagtaagc gagatcgtag
    14761 cggtttcttt gggcgaaaag aaatgcgaag aaacgttgcg taccgctttg gcgatgggtg
    14821 ccgaccgtgc aattcatgtt gaaaccgatg cgaaattgga gccgctggca gttgccaagt
    14881 tgctgaaagc cgttgcggac aaagaaaatc cgcagatttt ctttttgggc aaacaggcga
    14941 ttgatgatga tgccaaccaa gtggcgcaaa tgctggctgc tttgctgaat gcggcgcaag
    15001 gtacgttcgc gtccaaagta caaattgaag gcgacgaagt gcagattgtg cgggaaatcg
    15061 atggcggcga agaaaccatc gcattgaaac tgcctgctgt tatcagtgct gatttgcgtt
    15121 tgaacgagcc gcgctttgtc aaactcccca atattatggc ggcaaagaaa aaacctctgg
    15181 aaaaactggc gcctgccgat ttggctaccg atattttacc tcgtctgaaa acggtgaaat
    15241 ttgccgaacc taaggcgcgt caggcaggcg taaaagtagc aagcgttgcc gaattggttg
    15301 aaaaattgaa aaacgaagcc aaagtgattt gaggaggctg aaatgagcgt attgattatt
    15361 gtcgaacacg acaacaaaca gttgaatcct accactttgc atgctgttac cgctgccgcc
    15421 aagctgggta aagtcgattt attggttgcc ggaagcggtg catctgccgt agtagagtcc
    15481 gcaaaacaag tggcgggtgt ggaaaaagtt ttggttgcag atgctgccca ttatgccgaa
    15541 ggtttggctg aagagttggc tccgctggtt gttaaattgg cagcggatta ccgctatgtt
    15601 gcagcaacgg caaccacatt cggtaaaaac cttttgcccc gcgtagccgc cttattagac
    15661 gtaccgcaaa tttctgattt gaccgagatt gtagataaca cgacttttgt acgtccgatt
    15721 tatgcaggta atgcatttga aaccgtgcaa gccaatgcgg aaaaattggt gctgaccttc
    15781 cgtgcgacgg cttttgacgc agcggcggtg caaggtggta atgctgaggt aattaatatt
    15841 gaaacaaccc ctgcccaaaa cctgagtcgt tttgttaacc gtcagctttc ccattccgat
    15901 cgtcccgaac tgactcaggc aaaagtgatt gtttctggtg gtcgtgcgtt gggtagtgcg
    15961 gaaaaattta atgaagtgct gacaccgttg gcggatgttt taggtgcggc aatcggtgca
    16021 tcccgtgcgg cagttgatgc cgaatatgcg ccaaacgatg ctcaagtcgg acaaaccggt
    16081 aaagtagttg cgccgcaact ctattttgca atcggtattt caggtgcgat tcaacacgtt
    16141 gccggtatgc aagacagtaa ggtgattgtt gcaatcaata aagatgctga tgcgccgatt
    16201 ttcaatgtag ccgattacgg attggttggc gatttatttg aaatcgttcc acaattaaca
    16261 gaagcattga aaaattaatg tttcacgtga aactttcaga cgactttgtt attaatgagg
    16321 ttgtctgaaa acttttcaac atgattacga cttataaaac cattacttcc ccgacgcagg
    16381 ctgggtttaa agataaaggc agccgtttta tcgcatttgc ctatccgatt cggacattgg
    16441 ctgatgtgaa aaaatacctc gatccgttaa aggaagagca ccataaagca cgacactggt
    16501 gttatgccta tcgtttgggc gtggatggta tgcaatttcg tgccaacgat gatggagagc
    16561 catcaggaag tgctggacga ccaattttgg gacagattga ttcggtgggc atcaccgatg
    16621 ttttggtcgt ggtcgtccgc tatttcggtg gtactttatt gggcgtttca ggtctgatac
    16681 atgcgtacaa agaggcgacg gctcaggcgt tggcgattgc ggaagtgatt gaaaagaata
    16741 ttgaaaaaac cgtttggctg aaatgtgaat atccattttt gaatgaagcg atacgcatcg
    16801 ccaaacaata tcaggcggat atattggagc aggatttgca gttggattgc cgattgacgg
    16861 taagtttgtc gttggcaaat tatgaagtct gtgttgcggc ttggaagaat acacggcaga
    16921 ttgaagtaaa tacggaaaag ccttttgaat gaaaggtcgt ctgaaatgct ttgtgatggt
    16981 atttcagacg acctcttcgc tgactaatct tcttgtgtat atttgtctaa ggcatcgacg
    17041 ctggagcgca aatataacca agccagttga tggaattcgc ccagcgcgtt ccatagcgca
    17101 tcttcactca taatgctgta ttcgccttct gtgcgcaaat ccacctccgc tccatcttca
    17161 atagcccgat gacaagcctg atattcgtgg gtataaaaat tgtccatatc gcgaatcaaa
    17221 gcgacggcat gtttccaacg gcggatgtct tcttccaatt gcggtagtaa atgacaccat
    17281 tcgcggtatt tgctttggat ttcatcaata cgtttttgca tggttgcttg tcctttttta
    17341 atttgtgaat aagttgaata ggttgcagag tggaatgaag cggtcttttg tttgatttta
    17401 ctaaaatttc ttcagatggg ataaaagtcc ctttgtatcg agttttcgtt aaaagtacat
    17461 atttgtcaag gagatggcat gaaactgctg gttatcggta atggtggtcg cgaacacgcg
    17521 ctggcttgga aattggcgca gtcgcctaaa gtggaaacag tatttgttgc gcctggtaat
    17581 gccggtacag cgattgaacc caagttgcaa aacatcgact tgactgcgca tcaggatttg
    17641 attgaattct gtcgtaaaga aaatattgtt tttactgtcg tcggccctga agcgcctttg
    17701 gcggcaggtg ttgtggatga tttccgtgcc gcaggactaa aaatattcgg tccgacacaa
    17761 tatgcggcgc agttggaaag ttccaaagat ttcgccaaag catttatggc gaaatacaat
    17821 attccaaccg cgcaatatca aacctttgaa aacgccgatg ccgcacatga ttacgtcaat
    17881 cagaaaggtg cgcctatcgt tatcaaagcc gatggtttgg cggcaggtaa aggcgtgatt
    17941 gtggcgatga ctttggatga agcgcatgct gcgattgatg atatgctgct ggacaacaaa
    18001 atgggcaatg ccggcgcgcg tgttgtgatt gaagatttcc tgcaaggcga agaagcgagt
    18061 tttatcgtca tggttgatgg caataatgta ctgcctatgg caaccagcca agaccacaag
    18121 cgtcttttag acggcgacaa aggtctgaat acgggcggta tgggtgcgta cagccctgcg
    18181 cctgtggtaa cgcctgttgt gtacgaacgc gcgatgaatg aaattatttt gccgaccgta
    18241 gcagggatga aagcggaggg gcatgagttc accggtttcc tgtacgcagg tttgatgatt
    18301 gatcaaagcg gtgcacccta tacgattgag tttaactgcc gtttcggcga tcctgaaacc
    18361 cagccgatta tgagtcgtct gaacagcgac ttggcggatt tggttgaagc ggcaatagac
    18421 ggtaaacttg atagtgttgc tgccgaatgg aatccacaaa ctgccgtagg tgtagtgttg
    18481 gcggcgcaaa actatcctga aacgcctaaa aaaggcgatg ttatttccgg tctggatgct
    18541 gccaaccagg taggcaaagt tttccatgca ggtacaacgg caaacgagaa aggtgacgta
    18601 ttgaccaacg gcggacgcgt attgtgtgtt gtgggattgg gcgacaatgt ggcgcaagcg
    18661 aaagctaagg catatggcgc gttggaaaaa atcagcttcg acgggatgca ataccgtaaa
    18721 gacattgccg ataaagcgat taaccgttaa ttagaaagag gatgatgccg tctgaagcca
    18781 agtatgactg atatgcttca gacggcattt tatattatgt tatttccgag aattatcgct
    18841 gcatcagcgc aacgtaagtt aagcgtttat ttaaaaaaag gcggtttggt cgcttatccg
    18901 accgaatctt gttatggctt ggggtgtctt ccgacattag ccaaagcact cggtaagttg
    18961 gcgcatttga aaaaaagacc gcaacacaag ggtatgattg tcatcgggaa tcaatttgaa
    19021 caattgcagc ctttacttca aatgccgtct gaaaatcttc aggatatgct tagaaaagaa
    19081 tggcccgccc ctaaaacgtt cctattatcc gccaaatcct gtgtcttacc tgagttgcgc
    19141 gggaaacagc gcagcaagtt ggcggtcagg gttcccgccc atgtaggtgc gcgccgcttg
    19201 tgtcaggctt tgcaaacgcc tttggtttcg acttcgtgca accgcgcagg caaacgcgcg
    19261 tgtcggacgg agagggaagt cagaaggcaa ttcggacgcg atgtgtggat tgttggtggg
    19321 agaatcggca ggcagaaatc gccgagtcag attattgacg gggaaacagg taaacgtttg
    19381 cgttgagacc tttgcaaaat tcctttccct cccgacagcc gaaactcaaa cacaggtttt
    19441 cagctgtttt cgccccaaat accgcctaat tctacccaaa taccccctta atcctccccg
    19501 gatacccgat aatcaggcat ccggtctcct tttaggcgg
//
LOCUS       DAXHPG010000023        18392 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXHPG010000023 DAXHPG010000000
VERSION     DAXHPG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18392
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.023"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            138..572
                     /locus_tag="ACFM6F_000593"
     CDS             138..572
                     /locus_tag="ACFM6F_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC6164059.1"
                     /translation="MNFALSVITFTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(621..1964)
                     /locus_tag="ACFM6F_000594"
     CDS             complement(621..1964)
                     /locus_tag="ACFM6F_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC6164060.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTYTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIRLANDSPFGLGACIYTADTGCA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1969..2622)
                     /locus_tag="ACFM6F_000595"
     CDS             complement(1969..2622)
                     /locus_tag="ACFM6F_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC6164061.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQVRPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIVAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2773..3063
                     /gene="gatC"
                     /locus_tag="ACFM6F_000596"
     CDS             2773..3063
                     /gene="gatC"
                     /locus_tag="ACFM6F_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC6164062.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3126..4571
                     /gene="gatA"
                     /locus_tag="ACFM6F_000597"
     CDS             3126..4571
                     /gene="gatA"
                     /locus_tag="ACFM6F_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC6164063.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGVKIGLPKEYFGEGNSADVQTA
                     LQNTINLLKTQGAELVEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4568..5500
                     /locus_tag="ACFM6F_000598"
     CDS             4568..5500
                     /locus_tag="ACFM6F_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC6164064.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5543..6973
                     /gene="gatB"
                     /locus_tag="ACFM6F_000599"
     CDS             5543..6973
                     /gene="gatB"
                     /locus_tag="ACFM6F_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC6164065.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7065..8075
                     /locus_tag="ACFM6F_000600"
     CDS             7065..8075
                     /locus_tag="ACFM6F_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC6164066.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMDGHSEQALSEAEKAQGKILMCRTTAQSDISINIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRKNGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQNSSRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8204..8836)
                     /gene="pdxH"
                     /locus_tag="ACFM6F_000601"
     CDS             complement(8204..8836)
                     /gene="pdxH"
                     /locus_tag="ACFM6F_000601"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC6164067.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9067..10116
                     /locus_tag="ACFM6F_000602"
     CDS             9067..10116
                     /locus_tag="ACFM6F_000602"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6164068.1"
                     /translation="MSKQPTSKRQWRDGVAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(10185..11726)
                     /locus_tag="ACFM6F_000603"
     CDS             complement(10185..11726)
                     /locus_tag="ACFM6F_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC6164069.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVSTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVVETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYIAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(12019..13374)
                     /gene="xseA"
                     /locus_tag="ACFM6F_000604"
     CDS             complement(12019..13374)
                     /gene="xseA"
                     /locus_tag="ACFM6F_000604"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC6164070.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVAGKISIYETRGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAEERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGTGSEFQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKDTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            13571..14392
                     /gene="nadE"
                     /locus_tag="ACFM6F_000605"
     CDS             13571..14392
                     /gene="nadE"
                     /locus_tag="ACFM6F_000605"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC6164071.1"
                     /translation="MMRIFSTGGTMDTQAVITHIARWLDEYATQANAKGFVIGVSGGI
                     DSAVVSTLAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTADLTDTF
                     QTFEQTVGIHQTAFDNQPLSLANAKSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(14463..14936)
                     /gene="dnrN"
                     /locus_tag="ACFM6F_000606"
     CDS             complement(14463..14936)
                     /gene="dnrN"
                     /locus_tag="ACFM6F_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC6164072.1"
                     /translation="MTDFSVWETAPFGATVDYILQRYHNVHRAQFEELVPLAQKVVQV
                     HADTFPAEIARLLADMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            15234..15566
                     /gene="trxA"
                     /locus_tag="ACFM6F_000607"
     CDS             15234..15566
                     /gene="trxA"
                     /locus_tag="ACFM6F_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC6164073.1"
                     /translation="MSSELIIHTSDAAFEKDVLNSDTPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(15619..16566)
                     /locus_tag="ACFM6F_000608"
     CDS             complement(15619..16566)
                     /locus_tag="ACFM6F_000608"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6164074.1"
                     /translation="MTDITPFANRLGKNIKHLMKWVKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMLDILDIQRDHKKLIDGAVKLLASDGILYFSNNLSSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            16894..18282
                     /locus_tag="ACFM6F_000609"
     CDS             16894..18282
                     /locus_tag="ACFM6F_000609"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6164075.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
BASE COUNT         4629 a         5380 c         4647 g         3736 t
ORIGIN      
        1 gggaatctgt ttcaccccaa tatataaagc accatattaa ggcggagttt cttccccact
       61 ttgacccgaa cccggaaaag acaccgccca agccaatcct gatgctgccc cgacagccaa
      121 cccttaagga aaccctaatg aactttgctt tatccgtcat cacatttacc ctcgcctctt
      181 tcctgcccgt cccgcctgcc ggagccgccg tctttacttg gaaggacggc ggcggcaaca
      241 gctattcgga tgtaccgaaa cagcttcatc ccgaccaaag ccaaatctta aacctgcgga
      301 cgcgccaaac caaaccggcg gtcaaacccg cccaagccga cgcagggaag cgcacagacg
      361 gcgcggcaca ggaaaacaat cccgacactg ccgagaaaaa ccggcagctt gaggaagaaa
      421 agaaaagaat tgccgaaacc gaacggcaga acaaagaaga aaactgccgg atttcaaaaa
      481 tgaacctgaa ggcggtggga aattcaaatg caaaaaacaa ggatgatttg attcggaaat
      541 acaataacgc cgtaaacaaa tactgccgtt gagcagcctc atcgaaaacc ccatgccgtc
      601 tgaagcggca cggggtttgt ttatttctgc cagtaggttt tgacgttgac gaactcgtac
      661 agcccgaatt cggacaattc gcgcccgtaa ccggaatctt taacccctcc gaaaggcagg
      721 cgcaaatcgc tgctggtatg gcggttgata aacaccgatc ccgcctgtat tttttcggca
      781 aaccgccagg cgcatccggt atcggcggta taaatgcagg caccgagccc gaacggggaa
      841 tcattggcaa ggcggatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc
      901 ccgaatactt cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg
      961 ggataaaacc agcctcgccc ttgtgggatt tttccgccgg tcaggcatac cgcgccgttt
     1021 gaaacggcat cttcaacctg cccgtgaacc ctgtcccgca aatcttcgcg gtgcagcggt
     1081 gcaagcgtag tatcgggatg tttggggtcg cccattttca atttagcgca ttcggcaaga
     1141 aacagcgtga taaaacgatc ggctgcggct tcggttacga tgatgcgctt ggcggcgtta
     1201 cacgattgcc ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc acgctccaaa
     1261 tcagcatcgg gcatcacgat aaaggcgttg ctaccgccga gctccaacac ggttttctta
     1321 aggtttgcgc ccgcgtgtgc cgcaaggatg cgccccgtat acgttgaacc ggtaaacgcc
     1381 attgcatcgg tatcttcaac cgccttgagc gtgcccgcct catccagcca cacgcctgcc
     1441 agaggaatgc cgtctgaagc caaatcgaac agtgcctgac tgacgcgtgc cacgctgggc
     1501 gcgggtttga cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc
     1561 tgccagacgg gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcgaagcgc
     1621 acctgactca aactcgcctg cgtcgcgatg gttttgtggg caagcagttc gggggcaagg
     1681 cgggcgtaat agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcaag
     1741 cagcgtccga cttcctcaca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg
     1801 tcggcaaatt tttgcaggcg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg
     1861 ccgcgcattt tcaaatccgc cagccgccgt tcaaactccg cataatcttg agctggacgg
     1921 cggtaaagcg tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag
     1981 ttgcgggaat atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat
     2041 catacccatg atacggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt
     2101 cccggcaacg attaaaatgg cataacaaat cgcactgacc accaaaccgg ccacgataat
     2161 cagcgcgatg tcgctgtacg ttttggctgc cgaagcgtaa ataatcacgg tcgaaatacc
     2221 gcccgggccg atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg
     2281 ggcggggcgc acctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc
     2341 gttgccgttc atcatcgaaa tggcaatcag caacaccaaa attccgccgc cgacctgaaa
     2401 cgaaccgacg ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc
     2461 aatcacggca aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct
     2521 gtgcccgttg gtcaggtcaa ggtaaagcga caacgcgcta aacggattaa tcagcaccaa
     2581 aaaagccaca atcagcttgc cgatttccat gcccaatccc attatttccc cctccttcaa
     2641 acccgtgcgg caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa
     2701 ttaaaaattc aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca
     2761 aggatgcttt tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga
     2821 ctgcacctga ctgcggaaga aaaagaaaaa tcgcttcaag aattaaacga cattttcact
     2881 atggtcgaac agatgcaaac catcaacaca gacggcatcg aaccgatggc gcacccgcac
     2941 gaggccgccc tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat
     3001 caggcgggtg ctccggaagt acgcaaccgt ctgtacatcg taccgcaagt tatcgaagaa
     3061 taatccgaat atgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa
     3121 gatttatgac ccaatacaca ttgaaacagg caagcgtcct gttgcagtcc aaacagattt
     3181 ccgccgtcga actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca
     3241 acggctatat caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac
     3301 gtatcgcgca gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt
     3361 tctgccaaac cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc
     3421 cctacaccgc caccgtcgtc caaaacctgc tcgacgaagg tatggtaacg ctcggccgca
     3481 ccaatatgga tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggtgcagcca
     3541 aaaacccatg gaatcttgag cacgtccccg gcggttcgtc aggcggttcc gccgccgtcg
     3601 ttgccgcgcg cctcgcccct gccgcgctcg gttcggacac cggcggctct atccgccagc
     3661 ctgcatcgca ctgcggcatt accggcatca aacccacata cggcacggtt tcccgcttcg
     3721 gtatggtcgc ctacgcctcc agcttcgacc aggccggtcc gatggcgcaa accgccgaag
     3781 actgcgcgat tctgttgaac gcgatggcag gtttcgaccc caaagactcc accagcctcg
     3841 aacgcgaaaa agaagactac acccgcgatt tgaaccaacc gctcaaaggc gtgaaaatcg
     3901 gcctgcccaa agaatatttc ggcgaaggca acagcgccga tgttcagacg gcattgcaaa
     3961 atactatcaa cctcctcaaa acccaaggcg cggaattggt cgaagtttcc ctgccgcaaa
     4021 ccaagctgtc catccccgcc tactacgtcc tcgcctccgc agaagccagt accaaccttt
     4081 cacgttacga cggcgtacgt tacggacacc gtgccgccca attcggcgat ttggaagaaa
     4141 tgtacggcaa aacccgcgcc gaaggtttcg gcagcgaagt taaacgccgc atcatgatcg
     4201 gcacttatgt actgtcacac ggctactacg atgcctacta tctcaaagcc caaaaactgc
     4261 gccgcctcgt tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc
     4321 cgaccgcacc cactgcagcc ccaaaaatcg gagcggatgc ttcgccggtt gaaacctact
     4381 tgagcgatat ctacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg
     4441 caggcttcag cggcggcgga ctgcccgtcg gcgttcagct tgtcggcaac tacttcgccg
     4501 aagccaaaat cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac
     4561 gacccgaatg aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta
     4621 tgcgccttta tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa
     4681 agaaatttac gccttcgagc agtttgtcaa cgcatcccct atccgtcagg ggctgttcct
     4741 ccactgcccg caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg
     4801 caaacgccgt ttagatgcga tgacggcaga ttttctcatg gcggaaaaac tgttcggcac
     4861 agacatcctg caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga
     4921 cggcatcagc ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt
     4981 atctttgcgc gacgaagcag gcaaccggct gtatatggcg accttcgcct ttgtcggcac
     5041 acacctgctg acagcctccg tacaagggcc ggcgggtgaa gaagccaaag acaccgtccg
     5101 ccgcataacc aaacaactcc acggcttgcg tccccaacaa ctgatggtaa ccgccctgca
     5161 atatttcgcc gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt
     5221 caaactgcgc tggaaactta aaaagcgtgt caaaatgaat tacgacgcat tctggcagga
     5281 atacggcgca agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa
     5341 agaccttacc gacatcgaaa gcaaaaaacg ctcgatgtac cgcaagcgtt atgaaatgct
     5401 ggacaatatg gttgcagaga tgaaggacgg tctgaaaaca gaagcacgcg gcatttcaga
     5461 cggcatccaa acggaaaaac cgctccgccg gacagcctga cgcgaagact atcgaattga
     5521 tattttagag aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt
     5581 ccaattgaac accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc
     5641 caacgcgcac gccggcgtag tggaatgtgc tttgccgggc gtactgccgg taatgaaccg
     5701 agaagtcgtt gaaaaagcca tcaaattagg cttggctttg gatgcgaaaa tcaaccggaa
     5761 aaacgtgttc gaccgtaaaa actacttcta tcccgactta ccaaaaggct atcaaatcag
     5821 ccagttggac ttaccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt
     5881 gaaaaccatc aacgtaaccc gcgcccatat ggaagaagac gcgggcaagt ccgtacatga
     5941 aggcttgaac ggcgcgaccg gtatcgattt gaaccgcgcc ggcacgcctt tattggaagt
     6001 cgtatccgaa cccgaaatgc gctccgccgc cgaagccgtt gcctacgcca aagccttgca
     6061 cagcttggta acctggctga acatttgcga cggcaatatg gcggaaggtt cgttccgcgt
     6121 cgatgccaac gtatccgttc gcccgaaagg tcaagcggaa ttcggcacgc gccgcgagat
     6181 taaaaacctc aattccttcc gtttcttgga gcaggcgatt aattacgaag cagaagcgca
     6241 aatcgagatt ttggaagacg gcggcaaagt acagcaggca acgatgctgt ttgatcccga
     6301 aaaaggcgaa acccgcgtaa tgcgcctgaa agaagacgcg caagactacc gctacttccc
     6361 cgaccccgat ttgctgcccg tcatcatttc agatgcccaa atgcaaaaag ccaaagcaga
     6421 aatgcccgag ctgccgaaag aaatggcagc gcgtttcgtg gcggactacg gcgtgtccga
     6481 atacgacgcg cgcctgctca ccgcaagccg cgcacaggcc gcctattttg aagaagccgc
     6541 caaagagagc ggacaaggca agccgactgc caactggatg aacggcgaac tcgccgccgc
     6601 actgaacaaa gaaggcatgg aacttgccga cagcccgatt accgctccgc gcctcgccgc
     6661 gctggtaggc aaaatcgccg acggcacatt aagcagcaaa ttagcgaaaa aagcctttga
     6721 agccatgtgg gcagaacccg aagccactat tgccgaaatc atcgaaaaac acggtttgca
     6781 gcaaatgacc gataccggcg cgattgaagc catggtggac gaagtgctgg caaacaacgc
     6841 caaagccgtg gaacagttca aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca
     6901 agtgatgaag gccagcaaag gcaaagccaa ccccgcgcag gttcaagagc tgattaaagc
     6961 caaacttgcc tgacaagcaa gatgccgtct gaaacccaaa tccgacgttc agacggcatt
     7021 ttcacagtct gaccacatta aaacagcata aaaaacaata ttccatgaac cacaccgtta
     7081 ccctgcccga ccaaaccacc tttgccgcca acgacggcga aaccgttttg gccgctgccg
     7141 cccgacaaaa cctcaacctg ccccattcct gcaaaagcgg tgtctgcgga caatgcaaag
     7201 ccgaactggt cagcggcgat attcaaatgg acggacactc ggaacaggct ttatccgaag
     7261 cagaaaaagc gcaaggcaag attttgatgt gccgcaccac cgcgcaaagc gatatcagca
     7321 tcaacatccc cggctacaaa gccgatgccc tacccgtccg caccctgccc gcacgcatcg
     7381 aaagtattat tttcaaacac gatgtcgccc tcctgaaact tgccctgccc aaagccccgc
     7441 cgtttgcctt ctacgccggg caatacattg atttactgct gccgggcaac gtcagccgca
     7501 gctactccat cgccaatccg cccgaccaag aaggcatttt ggaactacac atccgcaggc
     7561 gcaaaaacgg tgtctgctcg gaaatgattt tcggcagcga acccaaagtc aaagaaaaag
     7621 gcatcgtccg cgttaaaggt ccgctcggtt cgtttaccct gcaggaagat agcggcaaac
     7681 ccgtcatcct gctggcaacc ggcacaggct acgcccccat ccgcagcatc ctgctcgacc
     7741 ttatccgcca aaacagcagc cgcgccgtcc atttctactg gggcgcgcgt catcaagatg
     7801 atttgtatgc cctcgaagaa gcacaagggt tggcatgccg tctgaaaaac gcctgcttca
     7861 cccccgtatt gtcccgcccc ggagagggct ggcagggaag aaaagggcac gtacaagaca
     7921 tcgcggcaca agaccacccc gacctgtcgg aatacgaagt atttgcctgc ggctctccgg
     7981 ccatgaccga acaagcaaaa aatctgtttg tgcaacagca taagctgccg gaaaacttgt
     8041 ttttctccga cgcattcacg ccgtccgcat cataattccc cggtataaaa aagattcagg
     8101 ctttccgttc ggaacacaaa aaacttcccg tccgtatttt ccccataaaa aatgccgtct
     8161 gaagcccgat tccggttttc agacggcata tgttttttcc tgttcaaggc gacagccgct
     8221 cgcgtatcca gccaccatcc agcaaacggt attggatgcg gtcgtgcagc ctgctcggcc
     8281 tgccctgcca aaactcaatc aaatcgggaa tcacaatata gccgccccaa tgcggcggac
     8341 gcggcacgtg caaaggatgt ttgagtccga ccgccgccgc ctttgccacc aataccgcct
     8401 tgttcggaat cacctcgctc tgcgcacttg cccacgcacc caaacggctc tgatacgggc
     8461 ggctctcaaa atattcgtcc gacaacttct ccgccagcct ttcaacacgc ccttccacgc
     8521 gcacctgacg ctccagctcc ggccaaaaaa acgtcatcgc cgcaaatgga tgagcatcca
     8581 gcgaacgtcc cttacggctg tgataattcg taaagaaaac aaaaccttca gaattaactt
     8641 ccttcagcag caccatacgg ctgttgggtc tgccgcgtcc gtcgaccgcc gccacattaa
     8701 ccgccgtcgg ctcgttgacc tgtgcgcgta ccgcctcgtc caaccaccgc tcgaactgct
     8761 cgatcggatt gtcggcgcaa tcgccttccg acaattcccg tttactgtaa tcttcccgaa
     8821 tattgtgcaa atccatttac cgctcctctt gtcatttgaa agattctacc cccgcacgca
     8881 aaccgatttc aaccgttgca caaactttgc ctcgcttctc caaacctcag gacacaacga
     8941 tttcatccgc aaaaccgccg cataaggtac aatatcgaac cgtccgaccg agggcggcat
     9001 tttatcaacc cgtcctgccg cacacgccgc agaagaaccg ccttatcagg cgagttagga
     9061 aaaatgatgt ccaaacagcc caccagcaaa cgccaatggc gcgacggcgt agccccgtct
     9121 gccaagaaaa ccgccaaacc tttcaaaagc aaagcccgtc ccaaagacga aacggacaaa
     9181 accgcatccc aaccttacgg acaaaaagct tcagacggca tcaaacctca aaacctcccc
     9241 aaacagcgcg ccgccaaagc caaaaaactc gtcgtccgca atcccaacca aaaaattatg
     9301 gaacacgcgc gcgatttaaa agaacgccgc agcgacctat cgcgcatgga acccgaacgc
     9361 ctgcaaaaag tgcttgccgc gtccggcgtc ggctcgcgcc gcgaaatgga agaatggatc
     9421 aacaacggct gggtaacggt caacggcaaa accgcgcaac tgggcgacaa agtcaccccc
     9481 gacgaccacg ttaccgtcaa aggcagcatc atcaaactca aatgggccga ccgcctgccg
     9541 cgcatcatcc tatattacaa acaagaaggc gaaatcgttt cccgcgacga cccgcaaggc
     9601 cgcgtcagca tattcgaccg cctgccgcag gccgccagca gccgctgggt cgccatcgga
     9661 cgcttggaca tcaacaccag cggacttctg attctcacca cctccggcga actcgtccaa
     9721 cgtttcgccc accccagctt cgaagtcgaa cgcgaatacg ccgtgcgcgt cttgggcggg
     9781 ctgaccggcg aacaaatgcg cgtcctcacc gaagaaggcg tgatgctcga agacggcttg
     9841 gcaaaagtcg aacgcatccg cgaacaaggc ggcgaaggcg cgaacaaatg gtacaacgtc
     9901 gtgattaaag aaggccgcaa ccgcgaagtg cgccgcattt ttgaaagcca agggctcacc
     9961 gtcagccgcc tcgtgcgcgt cggcttcggt cccatcggac tgcccaaccg cctcaaacgc
    10021 gggcagttct acgaactcaa ccccgccgaa gtcgccaaca tcatcaaatg ggcggacatg
    10081 ctgctgccgg gcgaacgccg ccgcaaaaaa gcctaaaccc gccaaaacac aaaaatgccg
    10141 tctgaaacat ctgctgtttc agacggcatt ttattcgggc gttttcagga gaaaaggtcg
    10201 agtgctttga caaagaccat caccacgccg taggcgagcg gtatgacggc caatgcccaa
    10261 cgccaccaga ccgatatgcc gccgccggaa actttttcgc ccacaaggta agcgtcggat
    10321 atggcggttt cgtcatcggg gttgccgctg tgtgcggcgg ttttgatgtc tttttcgtgg
    10381 tgtttttcgt gtacggattt gacggcgagg ttgcacaaca aaccgataat cagcagaccc
    10441 gccatgatgt acatggttac gctgtatgcc tgtgccgccg gtatgccgct gtcgatttgg
    10501 ctttggcgta tgtaattgac cagtaccggg ccgatgacgg cggcggttga ccaggccagc
    10561 aggatgcgcc cgtgaatcgc gccgacctga taggtgccga acaggtcttt caggtaggcg
    10621 ggaatggcgg caaatccgcc gccgtacatg gaaataatca cgcaaaagcc gatgatgaac
    10681 agggctttgc tgccgccctc gccgatggag ggaacggcga aatacagcag cgaaccgagt
    10741 acgaagaaga tggtgtaggt gtttttgcgt ccgattttgt cggaaacgct cgaccacaaa
    10801 aagcgtccgc ccatgttaaa caggctcagg aggctgacga agcctgccgc cgcacctgcg
    10861 ccgactgctg cctgcctgcc tatggaggtt tcggaaaaga gttcctgaat catcacggat
    10921 gcctgaccca atacgccgat gccggcagtt acgtttaggc acaataccca gaacaacagc
    10981 caaaactgcg gcgttttcat ggcttgggac acgttgacat gattgctgct gaccagcttg
    11041 ttttgcgttt tcggcgcgat atagccttca ggtttccagc cgtcggcagg tacgcggatg
    11101 gtaaacgcgc cgaacatcat cagtgcgagg taaagcagtc ccaatacggc aaaggtttca
    11161 acaaccccga ccgaagcggc gtttgaaaag gcgttcatca gtgatacgga aagcggtgag
    11221 gccagcattg cgccgccgcc gaaacccata attgccaaac cggtcgccat acccggcttg
    11281 tcgggaaacc atttcatcag tgtggaaacc ggcccgatgt agcctaagcc taagcctacg
    11341 ccaccgatga cgccgttgcc caaataaagc aggaagaggt tgtgcgtact cacgccgaat
    11401 gcggatacga agaagcccag gctgaaacag caggcggcgg caaatatggc tttgcgcggc
    11461 cctacccgtt ccatccatgt gccgaacagg gcggccgacg cgcccagcat cgcgagtgcg
    11521 atactgaaaa tccaacctac ggtcgtcagc ttccaatctc cggccgccga ttcggttatg
    11581 ccgataagtt tggtcagcgg cgcgttgaat acggaatagg cgtaaatctg cccgatggca
    11641 aggtgtaccg ccaatgctgc gggcggtacg agccaacggt tgaaacccgg cttggcaatg
    11701 cttgcctcac ggtctaaaaa cttcataaca tcctctttct gtcagttgaa aaataaaatt
    11761 tcatttgctc aatggaaact tattgaaaat tataaaaaaa tatcgggtcg ggtttttatc
    11821 cgccccaaga tgcgccgtct gaaacatttc gggtgtacgg aaaggtttct gttttttccg
    11881 acaaattcct gcggcttttc gcttccggat tcccgctttt gcgggaatga cgaattaaag
    11941 attatcttaa ggtcaaggga ctggattccc gctttcgcgg gaatgacggc ggagggggag
    12001 cggtttttcc gattgggttt aaatgcaatc gaacaaatcc tgctgccctt gttctttgct
    12061 tacgcgcacg tcggtttcgc cgtcggcgaa ggtaatgtgc agcttctgcc cctgcttcaa
    12121 aacatcggcg ttgcggatga cttgtccgcg tgtatctttg acgacggaaa agccgcgttc
    12181 caaaatgtgc tgcggcgaaa cggcttcgag caatgcggct tgggcggtca ggctttggcg
    12241 gcggtgggta agcagttggc tgaaggcggc gggcaatgcc gtctgaaggc ggacgatgtt
    12301 gtttttgcaa acggaaacat caggacggta atgttgcagg gcttgggttt ggcgttcaaa
    12361 acgggcggtg tgggcacgga ggttttgctt catagagtaa gacagcgttt gcgccagctt
    12421 gccgattgaa gcgcgctgct catcaagttt ttggcgcgga tgacggattt gccgcgcaag
    12481 ccagtcgagt ttttggctgg catcgaaata gcgttgttcc aaaacggttt tcagacggcc
    12541 ttgcgcctgg gcgagacgat gtagcgattc ttggcggttg gggctgacca gttccgccgc
    12601 gccggtcggc gtgggcgcgc gcacgtcggc gacaaaatcg gcaagcgtga aatcggtttc
    12661 gtgtcccacg ccactgacga cgggaaccgt gcaggcttcg attgcgcgta cgaccggttc
    12721 ttcgttaaac gcccacaaat cttcaatgct gccgccgccg cgacagacaa tcaacacgtc
    12781 gcattcggcg cgttgcgatg cggttttaat cgcttgggca atctgaaact cgctgcctgt
    12841 gccttgaaca ggtgtcggat aaacgataac gggaatttcg ggcgcgcggc gtttcaaggt
    12901 ggttacaaca tcgcgcaatg ccgccgccgc caaactggtt acgatgccga tacattgagg
    12961 gcggacgggc aacggttttt tgcgttcctc cgcaaacgcg ccttccgcct gcaactgcgc
    13021 cttcaaccgc tcataggctt cgtaaagctg ccccaaacct ttgagccgca cttcgttcac
    13081 ggtaatctga aattcgcccc gcgtttcata aatactgatt ttgcccgcca cttcgatatg
    13141 gtcgccttct ttcaaaggtt tcgccaaacg cgccgccgca cccttgaaca tcgcgcaacg
    13201 cacctgcgcg cggctgtctt tgagcgagaa ataataatgc ccgctggcgg cacgggtcag
    13261 gttggacact tcgcccgcaa tccacagacc ggcaaggtgg ttttccaaaa tacttttggc
    13321 aaatgcgttc aactcggaaa cggacaacac gtcagaatgg aaaaaatcag acatcgaatc
    13381 aatcaaatag taaaaaatat gaatatgttt tgaagcccaa ggcggcatag agccgcctaa
    13441 attgtcaaca atattataac acgcgccatc ttgccgcccg actttgcccg tatgactttt
    13501 ttaagcgggg aatgggaaaa atattcatca acctgcctgc aatctattca aattgaaccg
    13561 ccgacaagct atgatgcgga tattttcgac aggaggaacc atggatacgc aggcagttat
    13621 cacacatatc gcccgatggt tggacgaata cgccactcag gcaaatgcaa aaggattcgt
    13681 cataggcgtt tccggcggca tcgattccgc cgtcgtctcc acactcgccg cccgcaccgg
    13741 ccgccccacg ctgcttctgg atatgccgat acgccaacac cccggccagc ttgagcgggc
    13801 aaggctgcac atccgcaatc tgcaacggca atatgccaat gtaagcgcgc aaacggccga
    13861 tttgaccgac accttccaga cctttgaaca aaccgtcggc attcatcaga cggcatttga
    13921 caatcagccg ctttccctcg ccaacgccaa aagccgcctg cgtatgctga ccctgtacta
    13981 ctacgggcag atacacggac tgctggttac ggggacaggt aataagattg aagatttcgg
    14041 cgtgggattt tttaccaaat acggcgacgg cggcgtagac atcagcccga ttgccgacct
    14101 gaccaaaacg caggtttacc ggcttgccga agcattgggc gtggacgagg cgattcaaaa
    14161 agccccgccg accgacggcc tgtgggatac ggaacgcacc gacgaagaac agatgggcgc
    14221 aagctatccc gaactggagt gggcaatggg cgtgtacggc acgcgcaaac ccgaagattt
    14281 tgaagggcgg cagcgcgaag ttctggaaat ctatacgcgg ctgcaccgcg cgatgcagca
    14341 caaaatcaac ccgattcccg tgtgccgcat tccgcccgaa ttgttgggct gaaacgtgga
    14401 aatgccgtct gaagcggaaa aaccgtattt cagacggcat ggaaatatcc ggctcccgtt
    14461 ccttaagaat cgagtacgcg ggcaaacaga atatcgtttt ccaaatgaat gtggtcgttc
    14521 aaatcctcca ccatttcttt ggccaaagcg taaaggcgcg tccagcttcc gcaagcccct
    14581 tctggcggtt ggaaattgtc ggtcagctct ttgagccgtg cgatggcgcg gtcgtgttct
    14641 tcgtgttcgt gcatcatcac gctgatgggc attgccgcac cgcgtccgac accctgatta
    14701 atcatcggaa acagcatcct ttcctctttc atcatatgca tcagcagttc gttctgcata
    14761 tcggcaagca gcctggcaat ttccgccgga aaggtatcgg catggacttg gaccactttc
    14821 tgcgccagcg gcaccaattc ttcaaattgt gcgcggtgga cattgtggta gcgttgcagg
    14881 atataatcga cggttgcgcc aaagggggct gtttcccaaa cggaaaaatc agtcattgca
    14941 gtgttccttt tatagggttt cgggtttggt tttgaacatt catactttaa gaatctattt
    15001 aaacggagca tacaccgccc gcgcgtttct gtacagcttc aaaaacattc cttacacttt
    15061 gataataaaa gtaattttca aaaataaaat actgtccgaa ccgtttttta gaatttgcaa
    15121 aggcgattgg ggcggtacag aaaaactatt atcccgcccg cccacttgaa atttttatgc
    15181 ccaagcccta tcctgcacgc tatcgtgcca atcccaaccg aaaaggaaaa ataatgagca
    15241 gcgaattgat tatacacacc agcgatgccg cttttgaaaa agacgtttta aattcagata
    15301 cccccgtcct gctggacttt tgggctccgt ggtgcggccc ctgcaaaatg attgccccga
    15361 ttttggacga catcgccgcc gaatttgaag gccgtctgaa agtggtcaaa atcaacatcg
    15421 acgacaacga agccaccccg tcccgtttcg gcgtgcgcgg cattccgacc ctgatggtgt
    15481 tcaaaaacgg cgaagtcgtg gctaccaaag tcggcgcatt ggcaaaaggt cagctgaccg
    15541 cctttgtcga agcctccatc gcctgataaa gcgcaatcga aaaagccgcc ggaagattcc
    15601 ggcggctttt tcgcaccctt aagatttgtg gcggatttcc cagcacctat ggattttttt
    15661 gttgcggaaa tcttcgggaa cggattgttt ggaaatgtct ttgacggcgt attgttccga
    15721 taccaagtcg tctaagacga agctgctcag gttgttggaa aagtacaaaa tgccgtctga
    15781 agcgagcagc ttcaccgcgc cgtcaatcag ctttttgtgg tcgcgctgga tgtcgagaat
    15841 gtcgagcatc tttttgctgt tggagaagct gggcgggtcc atcacaatga ggtcgaaccg
    15901 cctgccttcc ccatatgccg tctgaagata ttgaaacacg tcggcgcgga cgattttgtg
    15961 tcgttccgta tcgatgccgt tcaattcaaa attgcgtttc gcccaatcaa gatatgtgtt
    16021 ggacaaatcg acggtttcgc tggatgccgc gccgccggtg gcggcataga cggtgaagct
    16081 gccggtgtag gaaaacaggt ttaaaaaacg tttgcccgcc gccgtttcgc cgactttttt
    16141 gcgcgtgttt cgatgatcca aaaaaagccc cgtatccaaa tatttatcaa ggttgaccca
    16201 aaacttgcgg ccgttttcgg tgatgacgaa atcgtcgccc gccttgccgg ttttctcgta
    16261 ctgctgcaaa cctttttggc gttcgcggcg tttgaggcgg atttgttcgg gcgcaaaacc
    16321 ggtaacgaaa gcgacggctt ccaagacttc ggcaagccac gcttcgtatt cttcgggccg
    16381 catcagccag ccggtatcgt attcctgaag gtggattcga tcgccgtaaa catcggcggc
    16441 aaaggggaat tgggggatgt cgcggtcgta aatgcgccag gcttcgatgc cgttgcgttt
    16501 cacccatttc ataaggtgtt tgatgttttt gcccaagcgg ttggcaaacg gtgtgatgtc
    16561 ggtcattggt ttcaggcgga ataaagtgga aaacggcaat tttactgtaa ttaacgcccg
    16621 attgcttgac cgtttcgggc aaaccctata ccatccgtcg cttatcttgt catacgaagc
    16681 catcgccttc caacctaaac cgcccttacg ggcgcgtttc ttctgttgct ttgattttgc
    16741 aaagcatatc tgtgcaggtt gccgtcgatg taaaccacaa gcaagccgct tgcgacaacc
    16801 ctgtaacttc acattccccg tatcgttacc cttccctgct tcaggccgtc tgaacctttc
    16861 ggacgcgggc gttgttgtct tccaaggata gccatgtcta ttaaatttgc cgatttgaac
    16921 cttgataaaa acattttgtc cgccgtcagc agcgagggtt acgaaagccc gaccccgatt
    16981 caggcgcaag ccattccgtt tgctttggaa ggccgcgaca tcatggcttc ggcgcaaacc
    17041 ggctccggca aaaccgccgc ctttctgtta ccgactttgc aaaaactgac caaacgcagc
    17101 gaaaaaccgg gcaaaggccc gcgtgctttg gtgttgaccc cgacccgcga actggcggct
    17161 caagtcgaga aaaacgcgct ggcgtatgcc aaaaatatgc gttggttccg caccgtcagc
    17221 atcgtcggcg gcgcgtcttt cggctaccaa acccgtgccc tgagcaaacc ggtcgatctg
    17281 attgtcgcca cgccgggccg tctgatggac ctgatgcaaa gcggcaaagt tgattttgaa
    17341 cgtttggaag tgctgatttt ggacgaagcc gaccgtatgt tggatatggg ctttatcgac
    17401 gacatcgaaa ccatcgtgga agcaacgccg agcgaccgtc agactttgtt gttctccgcc
    17461 acttgggacg gcgcggtcgg caaactggcg cgcaaactga ccaaagaccc tgaaatcatc
    17521 gaagtcgaac gcgtggacga tcaaggcaaa atcgaagaac aactgctgta ctgcgacgat
    17581 atgcgccaca aaaaccgcct gctcgatcat atcttgcgcg atgccaatat cgatcaatgc
    17641 gtgattttca cgtccaccaa agccatgacc gaagtcattg cggatgaact gtacgaaaaa
    17701 ggtttcgccg caaactgcct gcacggcgat atgccgcaag gctggcgcaa ccgcacgctg
    17761 atggatttgc gtaaaggccg ctgcaaaatt ttggttgcca ccgatgttgc cgcacgcggt
    17821 atcgacgtac cgaccattac ccacgttatc aactacgacc tgccgaaaca ggcggaagac
    17881 tacgtccacc gcatcgggcg caccggccgc gcaggccgca cgggtattgc gattacgttt
    17941 gccgaagtga acgaatacgt caaagtccac aaaatcgaaa aatacattaa ccgaaaactg
    18001 cccgaactga ccatcgaagg catggaaccg acccgcaaac gcaaatccgc aggcggcaag
    18061 ccgaaaggca aaggcggctg gggcgatcgt aaatccggcg gttggcgcgg cgatcataaa
    18121 ccgagcaaag aaggcttcgg cggcaaaacg cgcggcgaag gtttcaagaa agaaggcttt
    18181 aagagagacg gtttcaaaaa aaccggcgaa ggcttcaaag gcaaacgcaa agccggcgat
    18241 tcttttgcag gcaaaggcga acgccgttac aaagaccgct aagccccaac ctgccgcata
    18301 aaccaatgcc gtctgaaacc gatttcgagt ttcagacggc atttttgcaa tgtttcagca
    18361 ccgcccggct ttgataccca aaggattaat ct
//
LOCUS       DAXHPG010000024        18302 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXHPG010000024 DAXHPG010000000
VERSION     DAXHPG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.024"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(31..1557)
                     /locus_tag="ACFM6F_000610"
     CDS             complement(31..1557)
                     /locus_tag="ACFM6F_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC6164076.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVFRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPKYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(1669..3075)
                     /locus_tag="ACFM6F_000611"
     CDS             complement(1669..3075)
                     /locus_tag="ACFM6F_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6164077.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADM
                     NESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFSAMNTLTLAGLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            3359..4138
                     /locus_tag="ACFM6F_000612"
     CDS             3359..4138
                     /locus_tag="ACFM6F_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC6164078.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            4135..4836
                     /locus_tag="ACFM6F_000613"
     CDS             4135..4836
                     /locus_tag="ACFM6F_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC6164079.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNIE
                     PIVFEREIDTWKTEFFTNINAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            4833..6287
                     /locus_tag="ACFM6F_000614"
     CDS             4833..6287
                     /locus_tag="ACFM6F_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC6164080.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACEII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(6470..6955)
                     /gene="purE"
                     /locus_tag="ACFM6F_000615"
     CDS             complement(6470..6955)
                     /gene="purE"
                     /locus_tag="ACFM6F_000615"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC6164081.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARERGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7003..7719)
                     /locus_tag="ACFM6F_000616"
     CDS             complement(7003..7719)
                     /locus_tag="ACFM6F_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC6164082.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGKIVRLSNEVETLNGKVKALEHAKTHPSGRTYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAAALLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQAYPGSPAAKRAASAIRKR"
     gene            complement(7719..8387)
                     /locus_tag="ACFM6F_000617"
     CDS             complement(7719..8387)
                     /locus_tag="ACFM6F_000617"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6164083.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTVLALALPKHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(8398..8583)
                     /locus_tag="ACFM6F_000618"
     CDS             complement(8398..8583)
                     /locus_tag="ACFM6F_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC6164084.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            8726..10702
                     /gene="mutL"
                     /locus_tag="ACFM6F_000619"
     CDS             8726..10702
                     /gene="mutL"
                     /locus_tag="ACFM6F_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC6164085.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIGLALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCTTMLERLALAHPHIAFSLKRDGKQVFKLPTQSLPERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASDYPTGNKPDTRNAFGASGKTAPMP
                     YQAARAPQQHSLSLRESRAAMNTYAELYKKTDDIDLELSRLEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGKLQSQRLLIPVTFATSHEECAALSDHADALAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLSELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            10797..12917
                     /gene="dnaX"
                     /locus_tag="ACFM6F_000620"
     CDS             10797..12917
                     /gene="dnaX"
                     /locus_tag="ACFM6F_000620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC6164086.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYAPPALQLLGRAAAGSMRDAL
                     SLLDQAIAMGSGSVAEQDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAATSCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPETETAQTSVQTASAAAMPSESKTAEPVSNQENNDVPPWEDAPDEAQTAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETNAPLSETPSENPIQATPNNEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDNAEIPPPDWEHAAPADAEEENNADEGSNDENNTPS
                     APPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARATADK
                     KRLDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADPA
                     AQKILQAFGAQWQPESLELAANRP"
     misc_feature    11986..12025
                     /gene="dnaX"
                     /locus_tag="ACFM6F_000620"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            12997..13332
                     /locus_tag="ACFM6F_000621"
     CDS             12997..13332
                     /locus_tag="ACFM6F_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC6164087.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(14164..15210)
                     /gene="recA"
                     /locus_tag="ACFM6F_000622"
     CDS             complement(14164..15210)
                     /gene="recA"
                     /locus_tag="ACFM6F_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC6164088.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            15464..16228
                     /gene="aroD"
                     /locus_tag="ACFM6F_000623"
     CDS             15464..16228
                     /gene="aroD"
                     /locus_tag="ACFM6F_000623"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC6164089.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPIVTMSMGQTGTVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            16248..18263
                     /gene="rep"
                     /locus_tag="ACFM6F_000624"
     CDS             16248..18263
                     /gene="rep"
                     /locus_tag="ACFM6F_000624"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC6164090.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAEMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVVKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIIKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGVRIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQTEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGGPED"
BASE COUNT         4512 a         5094 c         4943 g         3753 t
ORIGIN      
        1 cgtactggtt tttgttaatc cgctacattt ctataataaa ccttctatgg gcagcaggga
       61 taggattttt gcggcgatgc gtttccaaag tttggcttcg ggttcgttcg ggtaggtttt
      121 tcgggtggcg ggatcgtgcc attgcaggcg gttgtgcctg tcgagggtaa cgcggtaggc
      181 gtatttgggt gtggtatcgg caagggtgcg ctccatctgt tctgcgattt tggggctttc
      241 gataacaacg cccatttcag tattgagccg tgcggaacgg gggtcgaggt tgaatgagcc
      301 gatgaagatg cgtttgccgt ccacaatgaa ggttttggca tgcaggctgg ttacggagct
      361 gccggtcagg cctttgtctt ttgtggcagg gacggcatgg ttgggttgca gctcgtagag
      421 tttgatgccg gctttgagca gcggttttcg gtatttgaca tagccggaat ggacggcggc
      481 aacgtcggtc gcctgtagcg agttggtcag gacggtaacg tctatgccgt cctgcaccag
      541 ttttgccagt gcgtctgtgc cggattttgt ggggacgaaa tagggtgaaa ccagatagac
      601 gcttttttcg ggctgtttga gcgcgtcttg cagccgcccg gcaatcggcg gtttgcggcg
      661 gtcgcggtcg agtccttttg cagggtcgtc gctgatgagg cgggtttgga cgctctgcca
      721 gtcgatgcgt cctgtctgta ttttttggta gaggggcgac tgttcgacgg tttcgcggta
      781 gcgcaggagc gcgtgtctgg acgtttcgtc gttgtatccg agtgcttgaa gacccttgcc
      841 gatgttgccg ctgcggatga tgcgcgtggc gttgtgggcg gaatggcttg cccagtagcg
      901 atcgaagtcg tgcgatactt cgccgacgac gctgccggta gcgaggatat ccaggtcggc
      961 gaaaacggtg tcctcaccga ctttgaagta ttcgtcgccg atattgcgtc cgccgagtat
     1021 ggtggcgcgg ttgtcggcgg taaaggattt gttgtgcatg cggcggttga ggcgggggaa
     1081 atcagtcagg tagccgagtg cgcgccattt gcggaagacg aaggggttga acaggcgcac
     1141 ttcgatattg ggatggctgt cgagggcgag caggaggtcg tccaatccgc gcgtgttgtt
     1201 gtcgtccaac agcaggcgta cgcgcacacc gcgttctgcg gcaaggtaca cgaggttgaa
     1261 cagcagtcgg ccggaaatgt cgttgcgcca gatgtagtat tgcaaatcga ggctgtgttc
     1321 ggcagattcg ataagggcgg cgcgggcggc aaaggcttcg tgggggtcgt tcagcagata
     1381 gatatcggat agcccgttgg tatgaggggt gtgccggatt tgcaggatgt tgtccaggcg
     1441 gacgggtttg gaagtattga aatgacggct ttccgtccgt tcttccagtg ggggcaacca
     1501 tgaagaacat gaacagagaa ggaggcataa aagggaaatt aggctgcgtg ttttcatcag
     1561 ggatatggtt tcagacggca ttgcctgtgt tttggggttg gcgcgcatgg aagtgcggca
     1621 tcataatcca aacgttgaaa cgggtaaaag ttttgcgtgc ggaccgcttt aggacggtgt
     1681 gttccgtgtc aggtttgcgc cgtctgaaac gtgcagacgc ttgaaaacca acgacgaggc
     1741 aagggtgatg ccgccgatgc tgagcagggt catacggaag gcggaatgca gacctgaaga
     1801 agccggtatc agaaatgtcc agtttttaag gattaatgcg ccgacaacaa tgcccatgct
     1861 gatggcaagc tgttggttga ccgccatcag gctgttgccg ctgccggtct gttgcgggcg
     1921 caaaccggcg agggtcagtg tattcatggc agaaaactgc agggagttgc acgcgccgat
     1981 cgccagcgag aggaaaatcc aaatccacag cgacgagttt ccgtcaggca gggcgagcag
     2041 catgatgaag gcggcaagca gcttggtgtt ccaaagcagt accgtgcggt agccgaaacg
     2101 tttcatgagc ggtgcaatca gcggtttgac cagcagcgaa gacagggcga cgggggcgac
     2161 cagccaaccc gacaggcttg cgccgaagcc gaaagcgact tgaaacatca ggggcatcag
     2221 aaaaggaatc gagctgatgc cgagacggct gaacagattg cccgccagtc ccagacggaa
     2281 agtgcgtatc agaaacaggt cggcggaata aatcggtttg gacgcggttt tcatatgtcg
     2341 gaaataacgg cgtgcaaaca gcagtccgcc gcacagcggc aacagtgcaa aatacggagg
     2401 cagcgcgtgc gacaggcttt ctgccgaaag taacaagagg cacgcggcgg cagaaaaaat
     2461 cagataacct ttgaagtcta aagagatatt gctgccttta atatcgggca tgatgttgcg
     2521 tcccaatatg aaacccaaaa gaccgatggg caggttgagc aggaaaatcc agtgccacga
     2581 agcgtattcg accagataac cgcccgccaa aggccctaaa accggtccga ttaatgcggg
     2641 cataaccgca taattgatgg cgttgagcag cttggacttg tcgtacacac gcaagatggt
     2701 cagacgcggt atcggaacca gcatcgaacc gccgatgccc tgaacgacac gggaaagcgt
     2761 caattcaaac agcgaacccg atgcggcgca caatgccgat ccgagcataa aaacggcaat
     2821 cgaaccgaaa aagacttttt tcgttccgaa cctgtccgcc aaataaccgc tcaaaggaat
     2881 cagcagggca accgtcagcg tataggcaac gactgccagt tgcatatcca gaggcgactc
     2941 attcatgtcg gcggcaattt caggcagtgc ggtatttaaa atggtcgcat ccaacatctg
     3001 cataaaaatg gcaattgcca gcagaagcgg cagccaaggg gatggtgcgc gggcggatag
     3061 ggtgtttttt tccatagggc gattgtaccc catccttgtg ccgttattgt tttcagatgc
     3121 tgtctgaatg ccgtcagagt cggcgtcctg aatgttcaca agcaaacgaa tcagcatttt
     3181 attgtaatga taattattat cgaaaattat cagagtaagg tacagtaagc gttatggggt
     3241 agtttgtaag aaaaaccgga ttatttttta aaattagact tgacccgcaa cagtcaatta
     3301 cttaaagtaa acgcttacct ttctacagag aaaaacgggt ttcccgttat caaaaaacat
     3361 gagcgcaacc attcccccaa aaatcatccg atacgacagc aatccgaccg atgtctattt
     3421 tttcggcact tgcgtccttg atctttttat gcccgaagca ggcatggatg ccattgccct
     3481 aatcgagcag cagggcatac gcgtccattt cccgatggcg caaagctgct gcggccagcc
     3541 tgcctattca tccggccatc cgaccgaagc ctttgatgtc gccagagcgc aactcgacct
     3601 tttccccgag gagtggccga ttgtcgtacc gtccggttcg tgcggcggta tgatgaaaca
     3661 ccattggccg acgctgttta aaggcagcga gtacgaggaa agggctgtgg attgcgccgg
     3721 ccgcatcatc gagtttaccc atttcctgct tgccatcggt ttcaaacccg aagacaaggg
     3781 cgaaccgctc aaagtcgccg ttcacacttc ctgcgccgcc cgccgcgaaa tgaatgtcca
     3841 tctttcaggc tggcaactga ttgacggtat ggaaaacgtc gaacgcatcg tccacgacca
     3901 cgaaagcgaa tgttgcggct tcggcggcac attctccgtc aaacaagccg atatttccgg
     3961 cgcgatggta accgacaaag tcgccgcgct gaaagaaacc ggcgcaaccg aaatcatcag
     4021 cgcggactgc ggctgtatga tgaacatcgg cggcaaaatc gccaaggacg agccggatat
     4081 gccgcgtccg aaacatatcg catccttctt gttggaacgc accggaggca aagcatgagc
     4141 gcgcgtgaaa atattttggc aaaactgaaa aaagccgatg ccttgccgat ggaagagcct
     4201 gcggttttcg attattaccg cgaaaagggc gtttcttggg acagcgaagc cgagcgtctg
     4261 aaacattggg ctgccgcaat gcgcgcggtc aaaaccgaaa tttattgggt gacgaaaagc
     4321 aattggatgc aggttttccg cgaagcggca gaaggcaagg gtttgaaaaa catcctgctg
     4381 cccttggcga ccgaacacgg acaaattgcc cgtgccgcat tggcgggcag caatatcgaa
     4441 ccgattgtct tcgagcgcga aatcgatact tggaaaaccg agtttttcac gaacatcaat
     4501 gcgggcttca gcggcgcgca atgcggcatc gcccgcaccg gcacgctgat gctgttttcc
     4561 agccccgaag aaccgcgtac tttgagtctc gttccgcccg tgcatttctg cctgttcgat
     4621 acgtccaaga tgtacaacga gtttcacaat gccgtcgaag gcgaaaaact ggtggaaaac
     4681 ggtatgccga ccaatgtatt cctgatttcc ggcccgtcca aaaccgcaga catccaactg
     4741 acgcttgctt acggcgcgca cggcccgcgc gatttggtca tcctcgccat cctgcccgac
     4801 cacatttccc ctgccgattt ggaggaaaac gcatgactac gcaaaccatc aaatttcaca
     4861 tgaagccgga aactttcaag caaaacgccg caatttccct tcaagacaag cctttgcgta
     4921 aaagcctgcg taccgcgatg gatatgctga tgaccaaacg caaagccgtt ttgaccgacg
     4981 aagaagagct gcaaagcttg cgcgatttgt gcgaacacgt ccgtcagcgc tcattgtcta
     5041 aattgcctgc cctgctggag cagctggaag aaaacctgac taagttgggc gtaaaagtac
     5101 actgggcgga aacaccggcc gaagcctgcg aaatcatcca cgacatcatc acagccaaaa
     5161 acggcaagct gatggtcaaa ggcaaatcga tggtcagcga ggaaatcgag ctgaaccatt
     5221 atcttgaagc aaaaggcatt aaagcggtag aaagcgactt gggcgagttc atcgtccaaa
     5281 tggcaggcga aaaaccgacc catattgtga tgcccgcaat ccataaaacc aaagaacagg
     5341 ttagcgaact gttccaccaa aacctcggta cgccgctgac agacgatgta gaccaactga
     5401 ccggcttcgc ccgtaaagca ctgcgcgata tttacagcac tgccgatgtc ggtttgagtg
     5461 gcgtaaactt tgccgttgct gaaacaggta cgctgtgtct ggtggaaaac gagggcaacg
     5521 gtcgcttgag taccaccgta ccgcccgtgc atatcgctat taccggcatt gaaaaagtgg
     5581 tggcgaaatt gtccgacatc ccacccttgt acagtctgct gccgcgctcc gccatcggtc
     5641 aaaacatcac aacctatttc aatatgatta ccggcccgcg ccgcagtgaa gaattagacg
     5701 gtccgcaaga aatgcacttg gttctgctcg acaacggccg cagccaggct tatgccgaag
     5761 accaaatgcg ccgcaccctg caatgtatcc gttgcggcgc gtgtatgaac cattgcccgg
     5821 tttatacccg catcggcggc gcggcatacg gcacaaccta tcccggtccg attggcgaga
     5881 ttatttcccc gcacctgtta ggcttggatg ccactcgcga cctgccgacc gcctgcacga
     5941 tgtgcggcgc gtgcgtggaa gtttgtccgg tacgcatccc gattaccgaa caaatgcagc
     6001 gtttgcgcgt tgaagcgcaa cgttcgccga ccgaaaccgt gccgcacccc atccgggggc
     6061 aaggcgcatc gcataccttc ggcgaacaaa tggcgtggcg cacattcaac ggtattttca
     6121 gcggcagcaa aacctaccgc gccttcggtt gggcagccac caagttccgc aacctgaccc
     6181 cgcgcaaaca gttgggttgg acgcaaaacc gcgtgccgat gaaaccggcg aagaaaaccc
     6241 tgcacgaact gatggcagaa aaaatgcgcc aaaaagaaca ggcataaaaa gttgttcgca
     6301 aaaatgccgt ctgaaacccg aaacagggct tcagacggca tttgtatagt ggattaacaa
     6361 aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggcaag gcgaggtaac
     6421 gccgtactgg tttaaattta agccactata tattcgcgga cggcgggttt taaatctgtt
     6481 ccaattccat actcaaaaca gcctgttcct gtttggctcg gaagtctgcc agtttttgcg
     6541 ccagttcggg ggtttcgttg gcgagcatgg aaacggcgaa caatgcggca tttgccgcgc
     6601 ctgcctcgcc gatggcgaat gtggcgacgg gtacgccttt gggcatttgt acaatcgata
     6661 aaagcgaatc ttcgccgcgc aggtatttgc tggggacggg tacgcccaaa acggggacgg
     6721 tggtcttggc ggcaaccatg ccgggcaggt gcgccgcgcc gcccgcgccc gcgataatgg
     6781 ctttgatgcc gcgctcccgt gcggtttcag cgtattggaa cattaaatcc ggggtgcggt
     6841 gtgcggaaac gacgcgcgcc tcatattcta cgccgaactc ttcaagaaac tgcgctgcct
     6901 gccgcataac gggccaatcg ctgttgctgc ccatgatgat gccgatttga atcataaatc
     6961 ctccttggtg cggatggggt aaaaagcgga aaaatggaaa aactatcgtt tgcgtatggc
     7021 tgaggcggcg cgttttgccg ccgggctgcc cgggtaagcc tgtatcagac tgcgccaagt
     7081 cgcccttgca atgtctttct gctgcaacct gtattggcat tcgccgattt tgaatatggc
     7141 ttcgggcgcg gttgggctgt ctttgaaacg gttggcgtaa cgccctccga tttcgatgac
     7201 ggattcgcag ttccccatac gcgccctgct ttgcagcaac aggtacatac tgcgttgcgc
     7261 gatgctgccg ccgtcgcctc cgtccgcgcc tttcaacagg gcggcagcgg cagaaaacct
     7321 gccgctttta tagtgtttga gcgcctgatt gtagaggttt tgtgcggttt caacggtatg
     7381 tgcggatgcg ctgccgcctt cggtattgag gtaatgctct ttcaacttgc ggtcatcgag
     7441 tttttggacg tatgtcctgc cggaagggtg tgtttttgcg tgttccagtg ctttgacttt
     7501 gccgtttaag gtttccactt cgttcgacag ccggacgatt ttgccttcca gatagtccaa
     7561 acggtcttgc agagtgggaa cgggataggg aatagcgtcc gaactacctg cctctgcctg
     7621 cggttcgggc cgcatatcct gaatattgcg ggaaacaggg gaagaacagg cggcggacac
     7681 agacagccaa atgataaaaa gcggtaattt ggtcttcatt attttttcag aagcagggtc
     7741 aaaccgtcgc cgacgggcag ggtgatgggg acgatgcgcg ggtcgttcgg caggttttga
     7801 ttaaaatctt tgaggatgcc gacgctgggc ggcgcatcgg aagccgcttc gcgcatcacc
     7861 cttccgttca acaaaatatt gtcgatggca atgatgccgc cttgccggac aagcttgagg
     7921 caacgctcga aatattgcgg cgtgggcggt ttgtctgcgt ctatcagtgc caaatcgtag
     7981 cttccggctt caccctgtgc aatcaaatca tccaatgtca gcaatgcggg ttgcagatgc
     8041 aggctgattt tatgtgccac gcctgcctcg ttccaaacct gacgcgccgt atcggtaaag
     8101 gttacattga tgtcgcaggc ggtaatccgc ccgtgttttg gcaacgccaa tgcaagcacg
     8161 gtgctgctgt atccggtaaa tacgccgact tccagatatt tttccgcgcg aatcagcttt
     8221 gccagccaaa ccaaaaccgc cgcctgttcg cgcgcaatcg ccattttgcc catacggtga
     8281 tgctcggtct tctcacgcag ccgcgtcaaa acgggatgtt cgggttcgcc gatggcgttc
     8341 aaatagtttt gcaggtccgg tgcgacattg gacagatggg tcgtcatttc ggtgggttca
     8401 gtcttggtaa taggtataag gttttttcgc tacttttgcc gcctcgaagt tttcctgttc
     8461 ttcgggattg agttcgacat cccacaaaag cccccggttt tccaaacgct gctgttccaa
     8521 ctcaggtttt tcttcaatca gacggttgag gaattgtgtg gcttcggatt ggtagtgata
     8581 catctttgcg ctccaatttt acgggatacg gcgtgattat actggtattt tccaaacggg
     8641 ataaacggct tttatcaaga atacaggcag aaaagtaagg ggttttatta tagaataaag
     8701 cgtttttgca acggaagcct accttatgcc acgcattgcc gccctgcccg accatcttgt
     8761 caaccaaatc gccgccggcg aagtggtcga acgccccgcc aacgccttaa aagaaatcgt
     8821 tgaaaacagc atagacgcag gcgcgacggc gattgatgtc gaactggcgg gcggcggcat
     8881 ccgtctcatc cgcgtcagcg acaacggcag cggtatccat ccagacgaca tcgggcttgc
     8941 gctccaccgc cacgccacca gcaaaatcaa aactctaaac gatttggaac acgttgccag
     9001 catgggcttt cgcggcgaag gtttggccag tatcgcctca gtcagccgcc taaccctgac
     9061 cagccgtcaa gaagacagtt cgcacgcgac ccaagtcaaa gcagaagacg gaaaactcag
     9121 cagccctacg gcagccgccc accccgtcgg caccaccatc gaagccgccg aactcttctt
     9181 caatacccct gcgcggcgca agttcctcaa atccgaaaac accgaatacg cccactgcac
     9241 caccatgctc gaacgccttg cactggcgca tcctcacatt gccttctcac tcaaacgcga
     9301 cggcaaacaa gtattcaaac tcccaaccca aagcttgcct gaacggattg ccgccattgt
     9361 cggcgatgat tttcagacgg cttcattgga aattgacagc ggcaatggcg cattgcggct
     9421 ctatggcgca attgccaagc cgacttttgc caaaggcaaa actgacaaac aatactgctt
     9481 cgtcaaccat cgttttgtcc gcgacaaagt gatgctccac gccgtcaaac aggcataccg
     9541 cgacgtattg cacaacgccc ttacccctgc tttcgtcctc tttctcgacc tgccgcccga
     9601 agccgtggat gtcaacgtcc acccgaccaa aaccgaaatc cgcttccgcg acagccggca
     9661 ggtgcaccaa ctcgtgttcc acacgctcaa caaagccctt gccgacacac gcgccgactt
     9721 gaccgaaagc gtcagcaacg caggcgaagt gttgcatgac attaccggcg ttacgcctgc
     9781 cccaatgccg tctgaaaacg acagcgaaaa tctgtttgat agcgcatccg actacccgac
     9841 aggcaacaaa cccgatacac gcaatgcctt tggtgcatca ggcaaaaccg cgcccatgcc
     9901 ttaccaagcc gcgcgtgcgc cgcaacaaca cagcctgtcc ctgcgcgaaa gccgcgcggc
     9961 catgaatact tacgccgagc tttacaaaaa aactgacgac atcgaccttg agttgagccg
    10021 attagagcag gcacgtttcg gcaatatgcc gtctgaaacg cctgctcccc aaacagatac
    10081 gccgctttca gacggcatcc catcccaatc cgaactaccg ccgctcggtt ttgccattgc
    10141 ccaattacta ggcatctaca ttcttgccca agccgaagac agcctgttgc tcatcgatat
    10201 gcacgccgcc gccgaacgcg tcaactacga aaaaatgaaa cgccaacgtc aggaaaacgg
    10261 caaactacaa agccaacgcc tgcttattcc cgtaaccttc gccacgtccc acgaagaatg
    10321 cgccgccctc tccgaccatg ccgatgcact ggcaggcttc ggcttggaat tgtccgacat
    10381 gggcggcaat acccttgccg tccgcgcagt tcccgccatg ctaggtaaag ccgacgtcgt
    10441 ttcactggcc aaagatgtat tgagcgaact tgcccaagtc ggcagcagcc aaaccatcga
    10501 agaacacgaa aaccgcatcc tcgccactat gtcctgccac ggctcgatcc gcgccggccg
    10561 ccggctcacc ctgcccgaaa tgaacgccct tctgcgcgat atggaaaata cgccgcgcag
    10621 caaccagtgc aaccacggca ggccgacttg ggtcaaactg actttgaaag aattggacgc
    10681 actgttcttg cgcggacagt aagccgaaag tgctagaata cgccgcccga gaccgccgtt
    10741 cagacggcat tccgacgcac cgacagaaac atcacgaccg aaaccaagag aaaaacatgg
    10801 cctatcaagt tctcgcccga aaatggcggc ccaaaacctt ttccgaccta gtcggtcagg
    10861 aacacgtcgt caaagccctg caaaatgcct tggacgaagg caggctgcac cacgcctacc
    10921 tgctgaccgg tacgcgcggc gtgggcaaaa ccaccatcgc ccgcatcctt gccaaaagcc
    10981 tcaactgcga aaacgcgcaa cacggcgaac cttgcggcgt atgcgaaagc tgtacgcaga
    11041 tcgatgccgg acgctatgtc gacctgctcg aaatcgacgc cgcctccaac acaggcatcg
    11101 acaacatccg cgaagtcttg gaaaacgccc aatacgcgcc gaccgccggc aaatacaaag
    11161 tctatatcat cgacgaagtg catatgcttt ccaaaagcgc gttcaacgct atgctcaaaa
    11221 cgctggaaga gccgcccgaa cacgtcaaat tcatccttgc cacgaccgat ccgcacaaag
    11281 tccccgttac cgtattaagc cgctgcctgc aattcgtctt acgcaatatg accgcgcagc
    11341 aggttgccga ccaccttgcc cacgtcctcg acagcgaaaa aatcgcctac gccccccccg
    11401 ccctgcaact tttgggacgc gccgccgccg gatcgatgcg cgatgccttg agcctgctcg
    11461 accaagccat cgccatgggt tcgggcagcg ttgccgaaca agacgtccgc caaatgatcg
    11521 gtgcggtcga taaacaatac ctgtacgaac tgctgacagg catcgtcaac caagacggcg
    11581 aagccctgct ggcaaaagca caggaaatgg ctgcgtgtgc cgtcggcttt gacaacgcct
    11641 tgggcgaact tgccatactg ctgcaacacc tcgccctgat acaggcagtg ccgaatgcct
    11701 tggcgcacga cgaccccgat tccgatattt tgcaccgcct cgcccaaacc ataagcggcg
    11761 aacaaatcca gctttactac caaatcgccg tccacggcaa acgcgacctg agcctcgccc
    11821 ccgacgaata cgccggcttt atgatgaccc tgctgcgtat gctggcgttc gcacccttgg
    11881 cggcaacatc gtgtgatgca aacgccgtga ttgaaaatac cgaacttcaa tccccatcgg
    11941 cacaaaccgc cgaaaaggaa accgccgcaa aaaagcccca accgcgccct gaaacggaaa
    12001 ccgcccaaac atccgttcag acggcatccg cagcggcaat gccgtctgaa agcaaaactg
    12061 ccgaacccgt ttccaatcaa gaaaacaacg atgttccgcc ttgggaagac gcgccggacg
    12121 aagcccaaac cgcagccggc acggcgcaaa cgtcggcaaa aagcattcag acggcatccg
    12181 aagccgaaac gccgcccgaa aaccaagttt ccaagaacaa ggcagccgac aacgaaacca
    12241 atgccccctt gtccgaaacg ccgtctgaaa accccattca ggcaacaccg aataatgaag
    12301 ccgttgaaac ggaaacattt gcacacgaag cccctgcaga acctttctac ggttacggct
    12361 ttccggataa tgactgcccc gtagaagaca acgcagaaat cccgccgccc gattgggaac
    12421 acgccgcccc tgccgatgcg gaagaagaaa acaacgccga cgaaggcagc aacgacgaaa
    12481 acaacacgcc gtccgccccg ccgcccgaat tttccaccga aaactgggca gccatcgtcc
    12541 ggcatttcgc ccgcaaactc ggcgcggcgc aaatgccggc gcaacactcc gcgtggacgg
    12601 aataccatcc cgacaccggc ctgatggttt tggcgatgac cgccgaagcc cgcgccaccg
    12661 ccgacaaaaa acgcctcgac aaaatccgcg acacccttgc ccaagcctac gggctgcaac
    12721 tcaccctgca aacccaagac tggcgcgacg aagccggcag ggaaaccccc gcgatgcagg
    12781 acaagcgcgt ccaagccgaa gacaggcaaa aagcacaagc attgctcgaa gccgaccccg
    12841 ccgcacaaaa aatcctccaa gcattcggcg cgcaatggca gcccgaatca ctggaattgg
    12901 cagcaaaccg gccgtaaaca gatataatgc cgcccgaacc cttcggacgg cattgccgtt
    12961 tccctcattc aatcaaaaca gacaggagta ttcagtatgt tcggaaaagc cggattaggc
    13021 ggcctgatga aacaggcgca gcaaatgcag gaaaatatga aaaaagcgca agccaaactt
    13081 gccgaaaccg aaatcgaagg cgaagcaggc aacggcctgg tcaaaatcac aatgacctgc
    13141 gcgcacgaag tacgcaaaat cgacatcagc cccgatttga ttcaagaagc cgccgacgac
    13201 aaagaaatgc tcgaagacct catcctcgcc gccctcaaat ccgcccgggg caaagccgaa
    13261 gaaaccgcaa acaaaacaat gggcgcattc acgcaaggtc taccccccgg agtgggcgac
    13321 ttcttccgct gatccccgac cgtcattccc acgcaggcgg gaatctagaa cgtagaatct
    13381 gagaaaccgt tttactcgat aaatttccgt gccgaggggt ctggattccc gccttcgcgg
    13441 gaatgacggc atcagtttgc aagattcggc gtgaacggta aaaacagtga gaatgataag
    13501 aacgcaaaaa cggcaagaat agcgggaatc ggcaggctga agcccaccct accattattt
    13561 acacatccgt accgcttaaa tgccgtctga aacttcgtca ttcccgtgaa aacgggaatc
    13621 caaccccgtg ggagcagaaa cttacacccc gtcattcccg cgaacgcggg aatccagtaa
    13681 ccgaaaaacc acaggaatct atcggaaaaa cagaaaccct cgaccgtcat tcccgcgaac
    13741 gcgggaatcc agtaaccgaa aaaccacggg aatctatcgg aaaaaacaga aaccctcgac
    13801 cgtcattccc gcgaacgcgg gaatctagaa cgtagaatct gagaaaccgt tttactcgat
    13861 aaatttccgt gccgacaggt ttggattccc gccttcgcgg gaatgacggc atattttttg
    13921 catttgatat aaaggggcgt ttgaattttg ttcagcaagt gcaaagtgtt gcacataaaa
    13981 aggcgcaggc ttcatccgta aagtcggcgc aggatagagg caaagcgggc gtaggtcggg
    14041 ctgtagcaac tgtatttttc accccgtcgg gcaaaaatac caaaactcaa atcaagccgt
    14101 tcggaggcgg ctcaaaaaaa cggtacttcg cagcagaagt accgtttatc gggatttcag
    14161 gttttattct tcggggcgtt cgccgtcggt ttcgtcctgc gtcccttcgg tgatgtgcat
    14221 ttctacgccg ttgagggcgc ggatttttgc gtcgatttca ttggcgactt cgggattttc
    14281 cttcagccag acgcggacgt tgtctttgcc ctgaccgatt ttcgcgccgt tgtagctgta
    14341 ccacgcgccg gatttgttga tgatgtcgtt tttcacgccg atgtcgatca attcgccttc
    14401 ccaactgatg ccttctccgt agaggatgtc aaactctgcc tgacggaacg ggggggcgac
    14461 tttgtttttg atgactttga cgcgggtttc gttgcccaat acctcttcgc cttttttgat
    14521 ggatccggtg cggcggatgt cgaggcggac ggaagaatag aatttcagcg cgttgccgcc
    14581 ggtggtggtt tcggggctgc cgaacattac gccgatcttc atccggattt ggttgatgaa
    14641 cacaaccagc gtgttggttt ttttgatgtg tccggtcagt ttgcgcaaag cctggctcat
    14701 caggcgcgcc tgcagtccga catggctgtc ccccatatcg ccttcgattt cggctttggg
    14761 gacgagtgcg gctacggaat cgacgactac catatctatg ccgcccgaac ggacgagtgt
    14821 gtcgcagatt tccaaagcct gttcgccggt atcgggctgg gacaggtaaa gctcttcgac
    14881 ttttacgccg agtttgcggg cgtaaacggg atcaaaggcg tgttcggcat cgacaaaggc
    14941 gcacacgccg ccgtttttct ggcattgggc gacggcttcg aggcagaggg tggttttgcc
    15001 ggaggattcg gggccgaaga tttcgacgat gcgcccgcgc ggcagaccgc cgactccgag
    15061 ggcgaggtct aatccgagcg atccggtgga aatgacttcg aggttttctt cctgctggct
    15121 gccgtccatt ttcatgatgg cgcctttgcc gaaacttttt tcgatttgcg ccagtgcggc
    15181 ggcaagggct ttgcttttgt cgtctgacat tggggttact ccggaacaaa tgcggtatgt
    15241 gggatgcggc gcaacacggg ctgcggcgcg ggatgtgtat cgttttcccg atgtgcgggc
    15301 tatcggtaat gctgcttcac ggggttgcca ttatcgcata tttccttgct tgccgatatg
    15361 cggcaggacg cggcggcttg tgccggaatg gaatctggat gccgtctaaa aggcggccgg
    15421 ctttgttata atggcggctg ttttttctgt gtgtgcctgt tttatgtgtt cctgccttgt
    15481 tgtcaaaaat accgttatcg gaagcggacg caccaaaatc gccgtgccgc ttgtcgcccg
    15541 cgatgctgcc gaactttccg ccgtacttga gcaaatcaaa aatatgccct tcgatattgc
    15601 ggagttccgc gccgactttt tggaatgcgc gggcagcatc ggcgaagtat tgcgccacac
    15661 gcagaccgtc cgcgacgcgc tgcccgacaa accgctgctg tttacgttca gacggcatgg
    15721 cgaaggcggc tcgttcccgt gttcggacga ttattatttt gaactgctcg acgcgctgat
    15781 cgaaagccgc ctgcccgaca tcatcgacat cgagctgttt tccggcgaaa ccgccgtccg
    15841 gcgcgccgtg gcaaatgctc aaaaaaacgg catcgccgcc ctactctgca atcatgagtt
    15901 tcaccgcacg ccgccgcaag aagaaatcgt atgccgtctg aaacagatgg aggactgcgg
    15961 cgcggacatc tgcaaaattg cggtgatgcc gcaaagcgcg gaagatgtgc tgactttgct
    16021 ttccgccacg ctcgaagcga aacggcttgc cgccaaaccg attgttacaa tgtcgatggg
    16081 acagacgggg acggtcagcc ggcttgccgg acaggtgttc ggctcaagca tcacgttcgg
    16141 ttcgggaacg caaaactccg cgccggggca aatcggcgta tccgccctcc gtgcggcact
    16201 cgactgtctc gaaagcggcg cagactgatt tcagacggca tcaaaacatg atgaaactca
    16261 atccccaaca gctcgaagcc gtccgttacc tcggcggccc cctgctcgtc cttgccggtg
    16321 caggcagcgg caaaaccggc gtaattactc aaaaaattaa gcatttgatt gtcaatgtcg
    16381 gctatctgcc gcataccgtc gccgcaatta cctttaccaa caaagccgct gcggaaatgc
    16441 aggagcgcgt cgccgaaatg ctgcccaagt cgcaaacgcg cgggctgaca atttgcacgt
    16501 tccattcttt gggcatgaag attttgcgcg aagaggcgaa ccatatcggt tacaaaaaaa
    16561 acttttccat cctcgattcc accgacagcg cgaaaatcat cggcgaactt ttgggcggta
    16621 cgggcaaaga agccgtattc aaggcgcagc accagatttc cttgtggaaa aacgatttaa
    16681 aaacgcctga agatgtcgtt cagacggcct ctaatgcgtg ggaacaacaa accgcacgcg
    16741 tgtatgcgag ctatcaggaa accttacaaa gctatcaggc agtggacttc gatgacttaa
    16801 tccgcctgcc tgccgtgctg ttgcagcaaa acagcgaagt gcgcaacaaa tggcagcggc
    16861 ggctgcgtta tctgctggtt gacgaatgcc aagatacgaa tacctgccaa tttgcgctga
    16921 tgaaactctt gaccggtgcg gaaggtatgt ttaccgccgt cggcgacgac gaccagtcca
    16981 tctacgcgtg gcgcggtgcg aatatggaaa acctgcgcaa aatgcaggaa aactatccgc
    17041 agatgaaggt cgtcaaactg gagcaaaact accgctccac cgcgcggatt cttaaaatcg
    17101 ccaacaaagt catcgaaaac aaccccaagc tgtttaccaa aaaactttgg tcgcaattgg
    17161 gcgaaggcga gccggtcaaa gtcgttgcct gccaaaacga gcaacacgaa gccgactggg
    17221 tcgtcagcca aatcatcaaa caaaaactca tcggcggcga caaaacccaa tacgccgatt
    17281 tcgccgtgtt ataccgcgga aagcatcagg cgaggatttt cgaggaagca ttgcgcggcg
    17341 tgcgcatccc ctaccggctc tccggcggac aaagcttttt cgacaaagcc gaaatcaaag
    17401 acgtgttgtc ttatgtgcgg ctgcttgcca accccaacga cgatcccgcc tttctgcgcg
    17461 ccgtaaccac gcccaaacgc ggcatcggcg atgtaacgct gggcaagctc aacgcctacg
    17521 cgcacgaaca cgaatgcagc ctgtatgaag ccgcgcaaac cgaagaagcc cttgccacgc
    17581 tgaacaatac caaccgccaa cacctgcaag cctttatgga tatgttcggc agctaccgcg
    17641 ccaaagccga aacaggcgaa gcaggcgagt tcatcaacag cctgctcgaa gaaatcgact
    17701 atgaaaacca cctgcttgcc agtgaagaag gcaaagctgg cgaaatcaaa tggcgcaacg
    17761 tcggcgattt ggtatcatgg tttgcgcgaa aaggcgggga agacggcaaa aacatcatcg
    17821 aactcgccca aaccgtcgcc ttgatgacgc ttttggaagg aaaagacgaa gaagaaaccg
    17881 atgccgtctc gctatccacg ctacacgccg ccaaaggttt ggagtatccc tatgttttcc
    17941 ttgtcggttg cgaagaaggt gttttgccgc acaacgacag catcgaagaa ggcaacgtcg
    18001 aagaagaacg ccgcctgatg tacgtcggca tcacccgcgc caaacgccag cttacgctga
    18061 cccactgcgt caaacgcaaa aaacaaggca tatggcagtt ccccgaaccc agccgattca
    18121 tagacgaaat gccgcaggaa gatttgaaaa tcctggggcg caaaggcggc gaaccgattg
    18181 tcagcaaaga agaaggcaga cgcaaccttg ccgatataat cggaaggctc gacagcatga
    18241 aaaaaggcgg tccggaggat taaaccaaga tagggaaacg aagccggaga caatgtatag
    18301 tg
//
LOCUS       DAXHPG010000025        17829 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXHPG010000025 DAXHPG010000000
VERSION     DAXHPG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.025"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..619
                     /locus_tag="ACFM6F_000625"
     CDS             <1..619
                     /locus_tag="ACFM6F_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6164091.1"
                     /translation="VSDYFRNIRAHSIHPRVSVGYDFGDWRIAADYASYRKWNNNKYS
                     VNTKEVGRNNNNNIDIWKELKTENQENGSFHAASSLGLSAIYDFKLNDKFDKFKPYIG
                     ARVAYGHVKHQVHSVRKETTTVFSKPSGSVTKPGEIVETPTSKPAYHESHSISSLGLG
                     VIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYRF"
     gene            complement(593..802)
                     /locus_tag="ACFM6F_000626"
     CDS             complement(593..802)
                     /locus_tag="ACFM6F_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164092.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKCA
                     GKGLMPSEGSDGIGIGESEAVAHAQ"
     gene            complement(820..1377)
                     /locus_tag="ACFM6F_000627"
     CDS             complement(820..1377)
                     /locus_tag="ACFM6F_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6164093.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1453..3087)
                     /locus_tag="ACFM6F_000628"
     CDS             complement(1453..3087)
                     /locus_tag="ACFM6F_000628"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6164094.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3365..4471)
                     /gene="serC"
                     /locus_tag="ACFM6F_000629"
     CDS             complement(3365..4471)
                     /gene="serC"
                     /locus_tag="ACFM6F_000629"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC6164095.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4836..5267
                     /gene="rimP"
                     /locus_tag="ACFM6F_000630"
     CDS             4836..5267
                     /gene="rimP"
                     /locus_tag="ACFM6F_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC6164096.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTISFDGKTVQIELGNIDKARLRPEFKF"
     gene            5295..6797
                     /gene="nusA"
                     /locus_tag="ACFM6F_000631"
     CDS             5295..6797
                     /gene="nusA"
                     /locus_tag="ACFM6F_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC6164097.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVDENQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6809..9697
                     /gene="infB"
                     /locus_tag="ACFM6F_000632"
     CDS             6809..9697
                     /gene="infB"
                     /locus_tag="ACFM6F_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC6164098.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKTAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQITGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHARLIRNNVVIHTGE
                     LSSLKRYKDDVKEVRMGFECGLMIKGFNEIMEGDQLECFDIVEVARTL"
     gene            complement(9908..11302)
                     /locus_tag="ACFM6F_000633"
     CDS             complement(9908..11302)
                     /locus_tag="ACFM6F_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HFC6164099.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELIILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDATRLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(11364..12713)
                     /locus_tag="ACFM6F_000634"
     CDS             complement(11364..12713)
                     /locus_tag="ACFM6F_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC6164100.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLIL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWVVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIILNDAPKWAVMLETEWQDGA
                     WFEGRLAQEWLDKGVAANREQVAALEAELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKTNDRT"
     gene            12856..13482
                     /locus_tag="ACFM6F_000635"
     CDS             12856..13482
                     /locus_tag="ACFM6F_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC6164101.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLVLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13546..14964)
                     /locus_tag="ACFM6F_000636"
     CDS             complement(13546..14964)
                     /locus_tag="ACFM6F_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6164102.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            15291..16019
                     /locus_tag="ACFM6F_000637"
     CDS             15291..16019
                     /locus_tag="ACFM6F_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC6164103.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(16078..16566)
                     /locus_tag="ACFM6F_000638"
     CDS             complement(16078..16566)
                     /locus_tag="ACFM6F_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC6164104.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(17324..17788)
                     /locus_tag="ACFM6F_000639"
     CDS             complement(17324..17788)
                     /locus_tag="ACFM6F_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC6164105.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         4692 a         4962 c         4492 g         3683 t
ORIGIN      
        1 agtaagcgat tatttcagaa acatccgtgc gcattccatc cacccccggg tgtcggtcgg
       61 ctacgatttc ggcgactgga ggatagcggc agattatgcc agttacagaa aatggaacaa
      121 caataaatat tccgtaaaca caaaagaggt gggaagaaac aataacaata acattgacat
      181 ctggaaagaa ctgaagacgg aaaatcagga aaacggcagc ttccacgccg cttcttctct
      241 cggcttatcc gccatttacg atttcaaact caacgataaa ttcgataaat tcaaacccta
      301 tatcggtgcg cgcgtcgcct acggacacgt taaacatcag gttcattcgg tgagaaaaga
      361 aaccacgact gttttcagta aaccaagtgg tagcgttaca aagccaggcg agatcgtaga
      421 aactccgacc agcaaacctg cctatcacga aagccacagt atcagcagct tgggtcttgg
      481 tgtcatcgcc ggtgtcggtt tcgacatcac gcccaagctg accttggaca ccggataccg
      541 ctaccacaac tggggacgct tggaaaacac ccgcttcaaa acccacgaag tctcattggg
      601 catgcgctac cgcttctgat tccccgatac cgatgccgtc tgaaccttca gacggcatga
      661 gacctttgcc tgcgcacttg gtgcgctggt cgcctccgaa catggcgcga cacccgacat
      721 ttccgccgaa cgcatcgggc gtttcatgaa tccggtttaa aacgcatgga aaaatgccgt
      781 ctgaaagcct ttcagacggc attgtgcttg agattccgtt taccaatgac tgacaaacgc
      841 ttccaaatcg gtattcttgg gcttatgcac ttcctctgtc ggcgtgccga ccatcatcag
      901 cccgatgatt ttatccttat ccgcacatcc gaaagcctca cgcaacaggg ggctgttgac
      961 ccacatcccc gtaatccaga cattgtcgaa accttgcgcc gttgccgcca gttgcagcgc
     1021 atacgccgca caacccgccg tcagcatctg ttcccattcc ggtttcggct taggcacatc
     1081 gcggttcggc gcaaacgtta ccccgataac catcggcgcc atattgccca ctttttccgc
     1141 ctttttcatc gcatcgtcgc cgaaattcaa ttcggcaacc gtttgcttca acacatcgcg
     1201 aaaacgttgc aatcctacct cgccttgaat cacggtaaaa cggaaggggc gcatattgcc
     1261 gtgatcggga acttgggttg ccgcctgaaa tatttgttcc aactccgccg catcgggggc
     1321 ggggggcttc agctttttgg aagatcggcg gttcgtcaat aattttaaag catccatatc
     1381 gttatactcc ggtcatcggt cgggtgttcg gacacatatg ccgtccgaag gcttcagacg
     1441 gcatatccgg catcagcgcg gacggcggca ggctgccaat atatccattt ccttccgata
     1501 ggtttggcta ttggaaatgt ccatcagccc caataccgtg ctgaaatagt ggtcgtgcga
     1561 atattcgttt tccgccgctt tttgtttgag gcattggaaa tctatgcccc cgtgttggcg
     1621 gaaggctttg gaaaaccaca taaccatcgg gatatgcgtc tgcccggaag gcgcgatggc
     1681 gtaaggcgcg gcgtgcaggt acatcccgtt ttcgcccaaa ctttcgccgt ggtcggaaac
     1741 ataatgcacc acgctttcca aatcgtcgcg gttttcaagt ttgcggataa ccttgtcgat
     1801 aaactggtcc acatacaaaa ccgtattgtc gtaagtgttg accagcgtgg cgcgggtgca
     1861 tttgttgatt tcgttggtgt cgcaggtcgg cgtgaatttg cgttcggctt cggtatagcg
     1921 ttcgtaatac gtcggcccgt ggctgccgat ggtatgcagg attaaaaccg cgtctttatc
     1981 gtttttgttg aggacttcgt cgaacttggt cagcaggata ttgtcgaggc actcgccgtt
     2041 gcggcagtat tcgggcaggt tgagcgaggt aacgtcggta ttcggcactt tgccgcacac
     2101 gcccttgcag ccggaatcgt tttccaacca agtaacttcc acaccggcgc gctgcacgat
     2161 gtccagcagg ttgtcttggt gttcggcttt gatttcgtca taatccgtgc ggtcgaaggt
     2221 tgagaacata cacggcaggg agtgcgcggt cgatgtgccg cagcttctga cctgcgggaa
     2281 attgacaatt tcatcgccgc gcgcggcaag cagcggcgta gtttggcggc tgtaaccgtt
     2341 caaaccccag ttggcggcac gcgtggtctc gcccacgacc agcaccacga aacggcgcag
     2401 gctgccggcc ggccggtttt gcacgaccgc catatccaat tgcgtataag gaatattgga
     2461 acgcttccaa tctttgtatt tcgacacgcc cgcgccgatg aaattagatg gcacaatcag
     2521 atgggttact gatttattgt tgcggaaaaa cgaggcgtaa tcctgatatt gcaacattgc
     2581 gatgcccaac gcgcacaaaa aggaaacggc ggcaagcaca aggcgcgtca aaagctcctt
     2641 ataccaaacg cggtatttaa ccttgacggc gatatacgcc agcgcgggca atacgcccaa
     2701 acatacaatc cacagcacat agcccggcgt aatcaggcgc gcgctttcgg cagccgtagt
     2761 ttgcaagaca ttattcaaca tcgacttgtt gaaatagata ttgaaaaata tttcttggta
     2821 agacaccgcc gcactgataa ccaatatcaa cggaatcaat accttatgca cgaaaggcag
     2881 ggcaatgacg tgaaaaacga aattacttaa aaaaaacagc accaccggca tcgtatagag
     2941 gaagatatcc gccccggtgc cgttaaaagg atgaagctcg acaactttgg caaaaaaggc
     3001 gtaattcaat accagcgagg aatacaggga aaggaaggca atcagcgcgg aagagccgag
     3061 cttcggcctc aggttcggtt ttatcatttg gaatgtgtcg gataagggtt ggaaaaggca
     3121 tccggcattt ggaatccgga ttattgaaaa agattcttaa ttataaggca acggagcaaa
     3181 gcagggcaag aaaacggaag gcgacgcagg ctgccggata cacatctcgg cacaaacagc
     3241 atcacgcccg gattgaaaag gacggatagc cgccggcagc acaatcttac cacctccaaa
     3301 acgccgccgg agaacgccga aacagccgat gccgtctgaa gccgcttcag acaacatcgg
     3361 gacatcaacc gtaacgccgt tggaaatcgc gcataaaatc tgccaaagcc cgcacgcctt
     3421 cgagcggcat cgcattgtag atgctggcgc gcatcccacc gacagactta tagcccttca
     3481 acaggcacaa gccctgcaat tcggcttcca gcacaaaacg gcggtcaagc tcctcatccc
     3541 ccgtttggaa cacgacattc attttagaac gcgcattcgg acggatacgg ttgatataga
     3601 aaccgccgct gccgtctatc gtctcataca aggtttgcgc cttcagccga ttgaccgctt
     3661 caattttttt cacaccgccc tgcgcctgca gccagcggaa caccagcccc gacatataaa
     3721 tcgcgtaagt tgacggcgtg ttgtacatac cttcgcggtt caaatgcgaa cggtagttga
     3781 acacatcggg aatatcgttc ggacaacgct cgagcaaatc ctcacgcaca atcaccaccg
     3841 taacccctgc cggcccgatg tttttctgtg cgcctgcgta aatcagtccg tagtcggcaa
     3901 catcaaactc gcgcgacaaa atctcgctgg acatatcgca caccagcggc ggcatgcctt
     3961 ctgaaaggcg cggcacttca cggtattgca gcccgttgac cgtttcattg acggcaaaat
     4021 ggacaaacgc cgaatcgggt gcaacatccc acgtttccac aggcggcagg tcgagatagt
     4081 cgaactgctc gccgccatgc gccgccaaac ggatttccgt atcggtcaaa cggctcatct
     4141 gttcataagc gatacggctc cagttgcccg ttaccaccgc gtcggcagtg cggaaaccgt
     4201 gtgccagatt catggctgcc atattaaatt gggttgttgc tccgccctgc agaaacaata
     4261 tcttatagtt gtcaggcact ttcaaaagct gcctcaaatc ctgttccgca tgatgcagga
     4321 tgctcaaaaa catttccgaa cggtggctca tcgccatcac aggaaaaccc gtaccgttgt
     4381 agtccaacat ttcctgccgc gccgtttcca acacggcttc gggcaatacg gcagggccgg
     4441 cggaaaaatt gtaaatcgga taaagagaca tgatgcagcc ttgattctga acaataaccc
     4501 gcccgatttt aggcttgcag caggctttgt gcaaggatgg aaaatacctg tcctccgccc
     4561 ggcttcatgc cgcccgaaca cggaaaacaa catcaatata ttgatttaca aacataaaaa
     4621 tcatgctagc gacaaataga tacatttgtt ttgtcaacaa tattcacgat ttcccattac
     4681 aaacctccct tacacccgct tttttccgtc ccaaaaacac aaaataaatc aacactttca
     4741 tttctccgca aaagcggtta taattacgcc gatttttcaa ctttgacgaa aaatggccac
     4801 ggggccattt ttttattatg tacataggga gcagcatgga tatccaaacc atcctcgaaa
     4861 aaaccctgcc cggcctgggc tacgaactgg tcgatttcga actgaccgcg caaggaacat
     4921 tgcgcgtgtt catcgacaaa gaaagcggca ttaccgtcga agactgcgca accgtcagca
     4981 accatttgag ccgcgtcttc atggttgaag acatcgacta caaaaacctg gaaatttcca
     5041 gccccggact cgaccgtccc ttgaaaaaag ccgccgactt cgtgcgcttt gccggtcaga
     5101 atgccaaaat caaaacccgc ctgccgatag acggtcagaa aaactttatc ggtaaaatcg
     5161 aaggctgcga aaacgatacc gttaccatat ccttcgacgg caaaaccgta caaatcgaat
     5221 tgggcaacat cgacaaagcc cgcctgcgcc ccgaattcaa attctaaaac acaacaatat
     5281 tggagatgtt caaaatgagt cgtgaaatgt tacagctggc agaagcactg gcaagcgaaa
     5341 aaaacgttga tgcggaagtc gtcttccaag cactggaatt cgccctgtct accgccgcca
     5401 agaaaaaggc agaccgcgaa cacatggatg tccgcgtcca aatcaaccgc gataccggcg
     5461 aataccaaac cttccgccgc tggctgattg tcgccgatga agactatacc tatcccgatg
     5521 tcgaaaaaac catcgaggaa atccaagagg aaattcccgg caccgccatc caaatcggcg
     5581 aatactacga agagcagctg cccaacgaag gcttcggccg ccaagccgcg caaaccgcca
     5641 aacaaatcat cctgcaacgc atccgcgatg ccgagcgcga gcagaatctg aacgagtttc
     5701 tcgccgtcaa agaagacatc gtgtccggca cggtcaaacg cgtcgaacgc cacggcatca
     5761 tcgtcgaagt cgttgccggc aaactggacg cgctgattcc gcgcgaccaa atgattccgc
     5821 gcgaaaactt ccgcagcggc gaccgcatcc gcgccctctt cctgcgcgtc gaagaaatcg
     5881 gcaacaccgg ccgcaaacaa gtcatcctga gccgtacttc cggcgatttc ctcgtcaaac
     5941 tgtacgccaa tgaagtacct gaaattgcag acggcatgct tgaaatccgc gctgtcgccc
     6001 gcgacccggg ccagcgtgcc aaagtcgccg tcaaagccaa cgaccagcgc atcgatccgc
     6061 aaggcacctg tatcggcgtt cgcggttcgc gtgtcaatgc cgtcagcaac gaattgtccg
     6121 gcgagcgcat cgacgttgtc ctttggtcgc ccgagcctgc ccaattcgtc atgagcgcgc
     6181 tctcacccgc cgaagtcagc cgcatcgtga ttgacgaaga caaacacgcc gttgacgtga
     6241 ttgttgatga aaaccaactg gcgctagcca tcggacgcgg cggtcaaaac gtgcgccttg
     6301 cttccgacct gaccggctgg cagctcaaca tcatgacttc cgccgaggca gacgaacgca
     6361 atgcggcaga agatgccgcc atccgccgcc tgtttatgga tcacttgaac gtggacgaag
     6421 aaaccgccga cgtactggtt caagaaggtt ttgcaacctt ggaagaagtc gcctatgttc
     6481 ctgccgccga actgcttgcc attgaaggat ttgacgaaga aatcgtcgat atgctccgca
     6541 accgcgcccg cgatgccatc ctgaccatgg cgattgccgc cgaagaaaaa ctgggcgaag
     6601 tgtccgacga tatgcgcaac ctcgaaggca tagatgccga tatgctccgc agccttgccg
     6661 aagcaggcat taccacccgc gacgacttgg cagagcttgc tgtggacgaa ctgattgaaa
     6721 tcaccggtgt aaacgaagaa accgcaaaag ccgtcatcct gaccgcacgc gaacactggt
     6781 ttaccgaaga caaataaagg gggtacagat gagtaacaca accgtagaac aatttgccgc
     6841 cgagctgaaa cgccccgtcg aagacctgtt gaaacagttg aaagaagccg gcgtcagcaa
     6901 aaacagcggc agcgattccc tgacgctgga cgacaaacag cttctgaacg cctacctgac
     6961 caagaaaaac ggcagcaacg gcggcaccat cagcatccgc cgcaccaaaa ccgaagtcag
     7021 caccgttgac ggcgtaaaag tcgaaacacg caaacgcgga cgcactgtca acattccttc
     7081 tgccgaagaa ttggcagcac aggtaaaagc cgcccaaacc caagccgcac ctgtccggcc
     7141 ggagcagacg gcagaagacg cggcaaaagc ccgagccgaa gctgccgcac gtgcagaagc
     7201 ccgtgccaag gcagaagcgg aagcggcaaa actgaaagcg gcaaaagcag gcaacaaagc
     7261 caaacctgcc gcgcagaaac ccaccgaagc aaaagccgaa accgcacctg ttgcggcgga
     7321 aaccaaaccc gccgaagaaa gcaaagcgga aaaagcccaa gccgacaaaa tgccgtctaa
     7381 aaaacccgcc gagcccaaag aaaaagccgc caagccgaaa cacgagcgaa acggcaaagg
     7441 caaagatgcc aaaaaaccgg cgaaacctgc cgcacctgcc gtgccgcaac ccgtggtcag
     7501 cgcagaagaa caggcgcaac gcgatgaaga agcacgccgt gccgccgcac ttcgcgccca
     7561 ccaggaagcc ctgttgaaag agaaacagga acgtcaggca cgccgcgaag ccatgaaaca
     7621 acaggcagaa caacaggcaa aaaccgcaca ggaagccaaa accggcagac agcgtcccgc
     7681 caaacctgcc gagaaaccgc aggcagccgc gccggccgtc gaaaacaagc ctgtcaatcc
     7741 ggcaaaagcg aaaaaagaag accgccgcaa ccgcgatgac gaaggtcaag gccgcaacgc
     7801 caaaggcaaa ggcggaaaag gcgggcgcga ccgcaacaat gcacgcaacg gcgacgacga
     7861 gcgcgtacgc ggcagcaaaa aaggcaaaaa actcaaactc gagccgaatc aacacgcctt
     7921 ccaagcaccg accgaacccg tcgttcatga agttttggtt cccgaaacca ttaccgttgc
     7981 cgatttggcg cacaaaatgg cggtcaaagg cgtggaagtg gtcaaagccc tgatgaagat
     8041 gggcatgatg gttaccatca accaatccat cgaccaagac accgccctga ttgtggtgga
     8101 agaactcggc cacatcggca aacctgccgc agccgacgac cctgaagcat tcttggacga
     8161 gggcgcggaa gcagtggaag ccgaagcatt gccgcgtccg cccgtcgtta ccgtgatggg
     8221 ccacgtcgac cacggcaaaa cctcgctgct ggactacatc cgccgtacca aagtggtaca
     8281 gggcgaagcg ggcggcatta cgcagcacat cggcgcgtac cacgttgaaa cccctcgcgg
     8341 cgtgattacc ttcttggaca ccccgggcca cgaagccttt accgctatgc gcgcacgcgg
     8401 tgcgaaagca accgacatcg tgattcttgt ggtcgccgcc gacgacggcg tgatgccgca
     8461 aaccatcgaa gcgattgccc acgccaaagc ggcaggcgta ccgatggtgg ttgccgtcaa
     8521 caaaatcgat aaagaagccg ccaacccaga gcgtatccgc caagagctga ccgcacacga
     8581 agtcgtgcct gacgaatggg gcggcgatgt acagtttatc gacgtttccg ctaaaaaagg
     8641 cctgaacatc gatgcattgc tcgaggcagt cttgctcgaa gccgaagttt tggaactgac
     8701 cgcacctgtc gatgcgcccg ccaaaggcat catcgtcgag gcgcgcttgg acaaaggccg
     8761 cggcgcggtt gccacattgc tggttcaaag cggcacgctg aaaaaaggcg atatgctgct
     8821 ggccggtacg gcattcggca aaatccgcgc gatggtcgat gaaaacggca aatccattac
     8881 cgaagccggt ccgtccatcc ccgtcgaaat cctcggcttg tccgacgtac cgaatgcggg
     8941 tgaagacgcg atggtattgg cggacgagaa aaaagcgcgc gaaatcgccc tcttccgcca
     9001 aggcaaatac cgcgacgtgc gccttgccaa acagcaggcg gcgaagctgg aaaatatgtt
     9061 caacaatatg ggcgaaaccc aggcccaatc tttgtcggtc atcatcaagg cagacgtgca
     9121 gggctcttac gaggctttgg cgggcagcct gaaaaaactg tccacagacg aagtgaaagt
     9181 gaacgtgttg cacagcggcg tgggcggcat taccgaatcg gatgtcaact tggccatcgc
     9241 ttcgggcgca ttcattatcg gctttaacgt gcgtgcagat gcctcttcgc gcaaacttgc
     9301 cgaaaatgaa aacgtggaaa tccgctacta caacatcatc tacgatgcca tcgacgacgt
     9361 gaaggcggcg atgagcggca tgctttctcc ggaagagaaa gaacagatta ccggtacggt
     9421 cgaaatccgt caggtcatct ccgtttccaa ggtcggcaac attgcaggct gtatggttac
     9481 cgacggtgtg gtcaaacgcg attcccatgc ccgcctcatc cgcaacaacg tggttatcca
     9541 taccggcgag ctgtcttctt tgaaacgcta caaagacgat gttaaagaag tccgtatggg
     9601 cttcgagtgc ggcctgatga ttaaaggctt caatgaaatt atggaaggcg accaactgga
     9661 atgcttcgac atcgtcgaag ttgcacgcac cctgtgattt tgatgcggaa aaatgccgtc
     9721 tgaagcgttc agacggcata cgaaacgggt tctgtatcat acagaacccg ttttttgtcg
     9781 caaatcggct tcagacagcc ctcttgcctt atcccgattt gaatctgact tgccatacaa
     9841 acaggcttca gacggcatta tagtgaatta aatttaaacc ggtacagcgt tggctcgcct
     9901 tgccgtacta tttgtactgt ctgcggctcg ccgccttgtc ctgatttaaa tttaattcac
     9961 tatattttcc cgacaggcgg atttgaagtt tctccacata tccggcgcgt tcggcgcggc
    10021 tggtttcggg gcggtgcgta ttgagcgacg accatttcca atgactgcgg gctttgttga
    10081 gttcgggcgg gagtctggtg gcgtcccacg gggctttgcg gctgtgcagc tcgatatccg
    10141 actgtgccgc gtgtccgcgc gtttgcagga cgtggagcaa atcgagggcg cgggcggcga
    10201 gcagggtcag ggtttcaggg tcggtgtgca aggtttggcg gccggagagt ttgtcggaaa
    10261 tggtgcgggt attgggcagg atgccgccca aaaagccgcc gattgccgta cccaatccga
    10321 gcgagccgcc gagtgtggcg atgtccagcc ccaagccgat gagtgcgccg gttgccgcgc
    10381 ccgtgccggt gcggatgccg tattgtttga gcaattcgct gtcgaacggg tcttggcgga
    10441 aggcttgcgg catccagtcg ccgccgtcga tttcgctgtg gtagaaacgg tagagggcaa
    10501 acagccgctg ctgcatctgc cgttcgagtt ggcgtatttc cgcctgcatg gtttgcagca
    10561 cggtggcggt atcctcgttt tcgtccactt cctgcctgaa ggcggcggca tcgagcagga
    10621 aatcggcgat ttcgcggcgc gcttcgccgt ccagccgctg ccattcgcgc cggcgcatgg
    10681 ctgtcaggcg gtcaagtgtg ctgcgttcgg gcaacatggt ggcgaggttt tcccacaggc
    10741 gtagttcgcc ttcaaaatca aaggcgacgg tgtcgaaccc tgcgaaaacg tgcaggtttc
    10801 tcctcgccag catggttgtc cacgattcgg gaggctgtcc gccggtaaag ttgaacacgg
    10861 gcataaccgg tttggcgcac catgaaagga taatcaattc atcccgatat ttgtcgagaa
    10921 cgggttcgcg cgcgtcgatg acgtacattg ccatatcgct ttgcaagact tgccgtaaga
    10981 ctttggcttc ctgattgaaa tcatggtgcg cgccgtggct gccgagaaac tgttgcagcc
    11041 gttcgatgcc gtctgaacga ttgtccgtat ggttttccag ccattccagc acgccgcccg
    11101 cgtcttcgag tccgggcgtg tcgtacagga aaaccagcgt gtctgcgccg tcgctgatgg
    11161 cggcttcttc gacatgacgc gtggtcgatg gggcgtttct gacttcgccg aaaccgctgt
    11221 cgcgcaaaag ggtacgcagg agcgaggttt tgccggtgtt ggtatgcccg acgacggcga
    11281 gggaaagggg ttgtttgttc atgatgtttt tgaagaatgg attttcagac gaccttttaa
    11341 ggatggcggc tttatggggc gtttcaagtg cggtcgttgg ttttcagacg gccttcctgc
    11401 gccgctctgt cgggttccag ccacgccgcg ccgcattcgg ccagcgcgtt acgccaatgt
    11461 tccagctttt ccgaaaggtc gtctgaaagc ccctgttccg ccaaaagctg caccaccgcg
    11521 ccgccctgcg ccgcttccga gagtcggaca atctgccgca acacaccccg gtccggcaca
    11581 gtttgggcgc gcacgccgat aagcagttgc gccggtttct gcttcaactc cgcttccagc
    11641 gcggcgacct gttcccgatt ggcggcaaca cctttatcca gccattcctg cgccagcctg
    11701 ccctcgaacc acgcgccgtc ctgccactcg gtctccaaca tgaccgccca tttcggtgca
    11761 tcgttcaaga tgattttcgg tgaaacggcg gacacggttt cccgacgcgt atccgcatcg
    11821 gtgattttgt tctgccagcg gcggatgacc gcctgataat agggcttttc caaatccaag
    11881 ccgttctcgc ttgttttcaa aaggatttta cacactaccc aagccagcag gcgcggcagg
    11941 atgccgtagc aggcgatact gccgaccagc agccccgacc aagcccgcgc atcggcaata
    12001 ttgccgttca gacgaccttc gatgaccgct cgcgcatcag gcacgggaaa acccagtttc
    12061 gcaggcagcc atgccaacat ttccaccagc cgtaccgaag acgaatcgcc caacagcgtg
    12121 ctttcccagt tgaacgtata ttgccgcacc aaaagcagca acaataccga caccagcatt
    12181 ccgagcagcg tgcagagcca caggctgtgc gacgttgcgc ctattttcca acgtaccgaa
    12241 ggttgccgcc actcgtccgc atacagccgc aacaccgcct gatttacagg gtctttgccc
    12301 cgaaaccacg tcgccggact gctgaaaaaa cgccccactt tcacgcgcag gaacaacatt
    12361 gccaaccata ctgccaacat aagcgtattc atgcccaaca cgcccgccaa aatcaggaag
    12421 aaattcagcc cctgattgtc cattagaaga taagtgcctg aaaatccggc ggtaaacatc
    12481 atcgatgccg ccaccaccca caaccagaac gaccccgcac gcacacgttc caacgtctcc
    12541 cgcagcatac ggttcctgtc aatcatctcc gcccgacgga tgattttttc ctccgtactg
    12601 ccgtccacgc ggcgcaaagc ctccgtcgcc tgcacgggat cgccgctgaa aataaagccg
    12661 ccttcttcca aaatacggac cagctcaacc agttttcggg atggattcaa cataaaatgc
    12721 cgtctgaaaa taaaaaacag attttaacac acgaattttc aagaatattc acagtgtagg
    12781 caaagagtaa atcccacaca gaggcaaaag tatcggcgta aactgactgc ctctactttc
    12841 ccgaaagatt gtgcgatgta tacaggcgaa cgcttcaata cttacagcca tttgagcggt
    12901 ttgattctgg cggcggcagg tttggtgctg atgctgctga aaaccatagg acacggggac
    12961 ggctaccgta tcttcagcgt atcggtttac ggcatcagcc ttcttctgct ctatttgagt
    13021 tcctcgctgt accacggaat tgcagccgga aaactgaaaa gcattttgaa aaaaaccgac
    13081 cactgcatga tttatgtcct gattgccgga agctacacac cgtttgcact ggtttcttta
    13141 agaaacgggc cgggctggac ggtattttca ctgtcctggc tgctggcggc tgcaggaatc
    13201 gcacaagaac tcaccatcgg acggaaaagc gaaaaacgtc tgctgtctat tgtgatttat
    13261 gtcgtcatgg gttggatggt cttggcggta atgaaatccc tgacagcctc actcccgtcg
    13321 gcaggactgg cttggctggc ggcaggcggt atgctgtaca gcgtcggcat ttactggttt
    13381 gtaaacgatg aaaaaatccg acacgggcac ggaatctggc atctgttcgt attgggcggc
    13441 agcatcaccc aatttgtcag tgtgtacggt tatgtaatct gaatgccgtc tgaaaaacaa
    13501 aacctcccga tcctgaagat tgggaggttt tctgtttgcc ggacatcagc ccttgtcgtg
    13561 gaactcgtgg aattcatacg gataagacaa atcccgaccc gcttttttct gtgccaaata
    13621 atcatcataa atggcacgga tttccttacg caacaaaaac agggctatca agttcgggat
    13681 aaccataaag ccgttgaaca tatcggacag gctccaaacc aaatcgactt tgccgagcgt
    13741 acctaaaacg atggcaagca gaaccaatgc gcgatagatg cccaagtgtc ttcccctgaa
    13801 aagaaaacgg atgttggact caccgaaata ataccagccg ataatggtcg tcaaggcgaa
    13861 gaaggtcaga cacacggcaa gcaattgcga accgaagccc ggaaatgcct tgttaaaggc
    13921 aaattgagta accgccgcgc cctgttcgcc cgaaaggttg gcatcggtca gcaggataat
    13981 cagcgcagtt gccgtacata ccaaaatcgt atcgataaac acaccgacaa atgccgccat
    14041 accttgctgc acaggatgct tcacatccgc agtcgcgtgg gcgtgcggag tcgaacccat
    14101 ccccgcctca ttggaaaaca gaccgcgcgc cacgccgaaa cgtatcgctt cgcgcatacc
    14161 gatacccgcc gcaccgccca aaacggcttc gggattgaag gcggcggtaa agatgtggtt
    14221 gaacatcggc acaatatggt cggaaaattc aaacaggata acgacggcgc acaaaatata
    14281 aacaaccgcc ataaacggca cgacaaattg ggcaatattg gcaatacggt tcacgccgcc
    14341 aatcacaacc atgcccgcaa ggacggcaag cacaataccg actgccaaag aaggcacatc
    14401 aaatgcaatg gtaacggcag aagcaatgga gtttgcctgt gtcgcattac cgataaagcc
    14461 caatgcgata atcaacgcaa tggaaaagaa accggacaaa aaacgcgccg ctcccctgcc
    14521 gattttcgga gtcagaccgt gggtgatgta gaacgccggc ccgccgatgt atttgccgtg
    14581 gctgacgacg cggtatttct gcgccagcag tgcctccgca aaaatcgtgg acatccccaa
    14641 aacggcagaa acccacatcc aaaaaatcgc gcccggcccg cctgcggtga tggcggtcgc
    14701 cacgccggcg acgttgcccg taccgatttg cgcggatatg gcaaccgcca acgcctgaaa
    14761 ctgcgataaa gacttgtcgt ctttatcgcc tttggcaaac aagccgccga atacggattt
    14821 gaatcccgcg cccagcttgg taatctgcgg cgcaccaaga tacagcgtaa aaaacaggcc
    14881 gatacccaaa agcgcgtaaa tcagcaggta gtcccaaagg aaccgattga ctgtacccac
    14941 cagaacagac aatatatttt ccataaaata aaccttatct tacaattaaa atgactgcct
    15001 tccaaaagac attccaataa ggaaacacgg cgagcagacc gtatttgccg caacagatgc
    15061 cttaaattgt caacaatcgg ggagaagctg cgccatcata ccgtaaaata tcgttaatta
    15121 aaacatttct ttatttttaa gcggaaagcg gaggaaatcg ctttcagaca gcatagacaa
    15181 cggcacggca taaaacagga tattttgggt acttgcaact tatgttaaaa tgccgaccgt
    15241 aaaaaatctg acaaaaacag attaattatt tgaaataaga aaggaaattt atggcaggcc
    15301 atagcaagtg ggcgaatatc cagcataaaa aagcccgtca ggatgccaaa cgcggcaaaa
    15361 tcttcacccg tttaatcaaa gaaatcaccg ttgcggcgcg tatgggcggc ggcgaccccg
    15421 gtgccaaccc gcgcctgcgc ctggctttgg aaaaagcagc cgaaaacaat atgcccaaag
    15481 acaatgtgca acgcgccatc gataaaggta cgggcaactt ggaaggcgtg gaatacatcg
    15541 agttgcgcta cgaaggctac ggcatcggcg gcgcggcttt gatggtggac tgcctgaccg
    15601 acaacaaaac ccgcaccgtt gcggacgtac gccacgcgtt taccaaaaac ggcggcaact
    15661 tgggtaccga cggctgcgtg gcgttcaact tcgtgcatca gggctatttg gtattcgaac
    15721 ccggcgttga cgaagacgcg ctgatggaag cggctttgga agccggtgcg gaagacgtgg
    15781 ttaccaacga cgacggttcc atcgaagtca ttaccgcgcc aaacgattgg gcgggcgtaa
    15841 aatccgcttt ggaggcggca ggttacaaat ccgttgacgg cgacgttacg atgcgcgccc
    15901 aaaacgaaac cgaactctcc ggcgacgatg ccgtcaaaat gcaaaaactg attgacgcgc
    15961 tggaagactt ggacgacgtg caagacgttt acacttccgc cgtattgaat ctggactgat
    16021 acgcagcaca gcagacatac aataaaatgc cgtctgaacc tttcagacgg cattgattta
    16081 tttagccctt gcccacgccc accaaaccgt caggacaacc gccagaaaat acgccgcgct
    16141 ccaaacaggc aaagcaaccc ctaacagata atccggttcg gcacaatttc cgaacccgcg
    16201 cacgacaggc tcgaaccaat caaacaaaga ccagcccttc aagcggaacg tccacggcgc
    16261 accgcacgaa ggagccgtac ccggcggcag cgactgcaac cacaactgat atgccgcaac
    16321 agaaataccc gtaacggccg gaatgctgat aaagacagca ccgaacaaac cgcccgccct
    16381 tcttcttggt ctgcacatca ggacaattgc cgtacacaat gcggttgcca aaacgcataa
    16441 ccgctgactg atacacaaaa cgcaaggctc catacccaaa acatactgtg ccgccaaaga
    16501 accggcaaat gcacagaccg aaacggcaaa cagcagccaa acggcttttc taaataacgg
    16561 ggtcattttc tcaacacacc aatcaaaata ccgatatgcc gattttgctg gatatatccc
    16621 gagatggcaa gggacgaatc gacaccggcg caggtgcgga cgatttcggt tttcccgata
    16681 tacacaagac ctgacaaggg acaaaacggc gatttgcccg cacaatgccc gcaaaaaaat
    16741 acatccggag gatttgaatt tcggcaaatt tagcgaatca aaagtttatt tcaatgaaat
    16801 tatatgattt ttttgaataa gcggattgac aggtttttga aggaatttgt taccggatag
    16861 ccatcgggca agttttttgc aaaatttgaa tcggtcgggt aaattttcaa aaaataattg
    16921 acagcggata agaaacggcg gataattctc gccgtcgagt tgcttgatgc agcttgattt
    16981 ttctcctcta tttctccttt gtagacttgg cacacattca accggatgtg tgcatttttt
    17041 tatctccgct ttttttgaaa tttaattact tttattgttt gaaattccat tctttaagaa
    17101 atttaacaag aaaaacgctt gctttttgac cggaaaagct gcataattcg ttccgttagc
    17161 tggttcgagt agtcagttaa tagtttctcc tctatttctc ctttgtagac ttggcacaca
    17221 ttctattgaa tgtgtgcatt tttttatctg aagcaacaag cctctgtgcg tgatgttgtt
    17281 atgtttcatt taggtgtcaa accgcatatc cggtctgaaa tattcaatcc aaatccaaaa
    17341 ccggattttc tttgacctcc tccatcacaa cataactcct actctccgaa gcggcaggca
    17401 gttgcaatag gatattgcct agcatatccc gataggcaga catatcgggc aaacgtactt
    17461 taatcagata gtcgtattcg cccgacacca agtggcattc cataatttgc ggaattttca
    17521 gcacttcttt tttgaaatct tcgaaaatat tgcccgattt ggattgcagc ttcagctcga
    17581 caaaaaccaa tagtggtttg cccaacagat ggggattgag gtgggcgtga tagccggaaa
    17641 tataatgttc ccgctccaaa cggcgcaccc tctctgtaac gggcgtggtg gacaagccta
    17701 ccttctcggc aagctccgtc atcgggatgc gggcattctg ttgaaggatc ttaagaatgc
    17761 ggaaatcgat tttatctagt tctttcattt ggattgcctt gtatttatta ttgattttaa
    17821 caaatagag
//
LOCUS       DAXHPG010000026        17726 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXHPG010000026 DAXHPG010000000
VERSION     DAXHPG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.026"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..228
                     /locus_tag="ACFM6F_000640"
     CDS             <1..228
                     /locus_tag="ACFM6F_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6164106.1"
                     /translation="HTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVEL
                     IAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
     gene            246..557
                     /gene="rpsJ"
                     /locus_tag="ACFM6F_000641"
     CDS             246..557
                     /gene="rpsJ"
                     /locus_tag="ACFM6F_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC6164107.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            678..983
                     /locus_tag="ACFM6F_000642"
                     /pseudo
     CDS             678..983
                     /locus_tag="ACFM6F_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1247..1891
                     /gene="rplC"
                     /locus_tag="ACFM6F_000643"
     CDS             1247..1891
                     /gene="rplC"
                     /locus_tag="ACFM6F_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC6164108.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1891..2511
                     /gene="rplD"
                     /locus_tag="ACFM6F_000644"
     CDS             1891..2511
                     /gene="rplD"
                     /locus_tag="ACFM6F_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC6164109.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2508..2822
                     /gene="rplW"
                     /locus_tag="ACFM6F_000645"
     CDS             2508..2822
                     /gene="rplW"
                     /locus_tag="ACFM6F_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC6164110.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            2828..3661
                     /gene="rplB"
                     /locus_tag="ACFM6F_000646"
     CDS             2828..3661
                     /gene="rplB"
                     /locus_tag="ACFM6F_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC6164111.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQVGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3668..3946
                     /gene="rpsS"
                     /locus_tag="ACFM6F_000647"
     CDS             3668..3946
                     /gene="rpsS"
                     /locus_tag="ACFM6F_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC6164112.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3955..4284
                     /gene="rplV"
                     /locus_tag="ACFM6F_000648"
     CDS             3955..4284
                     /gene="rplV"
                     /locus_tag="ACFM6F_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC6164113.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4294..4986
                     /gene="rpsC"
                     /locus_tag="ACFM6F_000649"
     CDS             4294..4986
                     /gene="rpsC"
                     /locus_tag="ACFM6F_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC6164114.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4970..5386
                     /gene="rplP"
                     /locus_tag="ACFM6F_000650"
     CDS             4970..5386
                     /gene="rplP"
                     /locus_tag="ACFM6F_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC6164115.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5386..5577
                     /gene="rpmC"
                     /locus_tag="ACFM6F_000651"
     CDS             5386..5577
                     /gene="rpmC"
                     /locus_tag="ACFM6F_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC6164116.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5577..5840
                     /gene="rpsQ"
                     /locus_tag="ACFM6F_000652"
     CDS             5577..5840
                     /gene="rpsQ"
                     /locus_tag="ACFM6F_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC6164117.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            6063..6431
                     /gene="rplN"
                     /locus_tag="ACFM6F_000653"
     CDS             6063..6431
                     /gene="rplN"
                     /locus_tag="ACFM6F_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC6164118.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6443..6766
                     /gene="rplX"
                     /locus_tag="ACFM6F_000654"
     CDS             6443..6766
                     /gene="rplX"
                     /locus_tag="ACFM6F_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC6164119.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6776..7315
                     /gene="rplE"
                     /locus_tag="ACFM6F_000655"
     CDS             6776..7315
                     /gene="rplE"
                     /locus_tag="ACFM6F_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC6164120.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7318..7623
                     /gene="rpsN"
                     /locus_tag="ACFM6F_000656"
     CDS             7318..7623
                     /gene="rpsN"
                     /locus_tag="ACFM6F_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC6164121.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7639..8031
                     /gene="rpsH"
                     /locus_tag="ACFM6F_000657"
     CDS             7639..8031
                     /gene="rpsH"
                     /locus_tag="ACFM6F_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC6164122.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            8045..8578
                     /gene="rplF"
                     /locus_tag="ACFM6F_000658"
     CDS             8045..8578
                     /gene="rplF"
                     /locus_tag="ACFM6F_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC6164123.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRTFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8592..8945
                     /gene="rplR"
                     /locus_tag="ACFM6F_000659"
     CDS             8592..8945
                     /gene="rplR"
                     /locus_tag="ACFM6F_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC6164124.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8964..9482
                     /gene="rpsE"
                     /locus_tag="ACFM6F_000660"
     CDS             8964..9482
                     /gene="rpsE"
                     /locus_tag="ACFM6F_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC6164125.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9475..9660
                     /gene="rpmD"
                     /locus_tag="ACFM6F_000661"
     CDS             9475..9660
                     /gene="rpmD"
                     /locus_tag="ACFM6F_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC6164126.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLCRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            9662..10096
                     /gene="rplO"
                     /locus_tag="ACFM6F_000662"
     CDS             9662..10096
                     /gene="rplO"
                     /locus_tag="ACFM6F_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC6164127.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            10108..11418
                     /gene="secY"
                     /locus_tag="ACFM6F_000663"
     CDS             10108..11418
                     /gene="secY"
                     /locus_tag="ACFM6F_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC6164128.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11423..11641
                     /gene="infA"
                     /locus_tag="ACFM6F_000664"
     CDS             11423..11641
                     /gene="infA"
                     /locus_tag="ACFM6F_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC6164129.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11662..11775
                     /gene="rpmJ"
                     /locus_tag="ACFM6F_000665"
     CDS             11662..11775
                     /gene="rpmJ"
                     /locus_tag="ACFM6F_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC6164130.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            11841..12203
                     /gene="rpsM"
                     /locus_tag="ACFM6F_000666"
     CDS             11841..12203
                     /gene="rpsM"
                     /locus_tag="ACFM6F_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC6164131.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12223..12618
                     /gene="rpsK"
                     /locus_tag="ACFM6F_000667"
     CDS             12223..12618
                     /gene="rpsK"
                     /locus_tag="ACFM6F_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC6164132.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12638..13258
                     /gene="rpsD"
                     /locus_tag="ACFM6F_000668"
     CDS             12638..13258
                     /gene="rpsD"
                     /locus_tag="ACFM6F_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC6164133.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13284..14270
                     /gene="rpoA"
                     /locus_tag="ACFM6F_000669"
     CDS             13284..14270
                     /gene="rpoA"
                     /locus_tag="ACFM6F_000669"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC6164134.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14294..14662
                     /gene="rplQ"
                     /locus_tag="ACFM6F_000670"
     CDS             14294..14662
                     /gene="rplQ"
                     /locus_tag="ACFM6F_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC6164135.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14809..15522
                     /gene="minC"
                     /locus_tag="ACFM6F_000671"
     CDS             14809..15522
                     /gene="minC"
                     /locus_tag="ACFM6F_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC6164136.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15551..16366
                     /gene="minD"
                     /locus_tag="ACFM6F_000672"
     CDS             15551..16366
                     /gene="minD"
                     /locus_tag="ACFM6F_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC6164137.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            16370..16633
                     /gene="minE"
                     /locus_tag="ACFM6F_000673"
     CDS             16370..16633
                     /gene="minE"
                     /locus_tag="ACFM6F_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC6164138.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16637..17557
                     /gene="oxyR"
                     /locus_tag="ACFM6F_000674"
     CDS             16637..17557
                     /gene="oxyR"
                     /locus_tag="ACFM6F_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC6164139.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
BASE COUNT         4845 a         3421 c         4377 g         5083 t
ORIGIN      
        1 cataccccgt tcttcgctaa ctaccgtccg caattctact tccgtaccac cgacgtaact
       61 ggcgcggtta ctttggaaga aggtgtagaa atggtaatgc cgggtgaaaa cgtaaccatc
      121 accgtagaac tgattgcgcc tatcgctatg gaagaaggtt tgcgctttgc gattcgcgaa
      181 ggcggccgta ccgtgggtgc cggcgtggtt tcttctgtta tcgcttaatt gaaggatatt
      241 gataaatggc aaaccaaaaa atccgtatcc gcctgaaagc ttatgattac gccctgattg
      301 accgttctgc acaagaaatc gttgaaactg caaaacgtac cggtgcagtt gtaaaaggcc
      361 cgattccttt gccgaccaaa atcgagcgtt tcaacatttt gcgttctccg cacgtgaaca
      421 aaacttcccg tgagcaattg gaaatccgca cccacttgcg cctgatggac atcgtggatt
      481 ggaccgataa aactaccgat gcgctgatga agctggattt gccggccggt gttgatgtag
      541 aaatcaaagt ccaataattc ggactattga aaatccccaa gcaatcaatg cttggggatt
      601 ttttatgtta tgccgagacc tttgcaaaat tccccaaaat cctctaaatt cccaccaaga
      661 catttagggg atttctcatg agcaccttct tccagcaaac cgcccaagcc atgattgcca
      721 gacacatcga ccgtttcccg ctattgaagt tggaccaggt gattgattgg cagccgatcg
      781 agcagtacct gaaccgtcaa aaaacccgtt acctccgaga ccaccgcggt cgtcccgcct
      841 gtcccctgtt gtccatgttc aaagccgtcc tgctcggaca atggcacaac ctctccgatc
      901 ccgaactcga acacagcctc atcacccgca tcgatttcaa cctgttttgc cgttttgacg
      961 aactgagcat ccccgattac agtcatcaac catattccgg tttgtcggag aaagatgcat
     1021 acgctgtgat gaccggatac cgacccttta aaagagtccg accctatgcc gtctgaaaat
     1081 tcaaaacgct tcagacggca tattgaagat atttctgata tttctgttga tatttcttta
     1141 acttgtcaga tataatgccg agcttggtac atttgtgcca agtttaactt tgtctgaaag
     1201 acaggccaat cgcagcctgt ccctttactt taaaaggaaa ataatcatga ctttaggtct
     1261 ggttggacgc aaagttggta tgacccgcgt gttcgacgaa cagggtgttt ctgttccggt
     1321 aaccgttttg gatatgtcag ccaaccgcgt cacacaagta aaatccaaag atactgacgg
     1381 ctatactgcc gttcaagtta cctttggtca gaaaaaagcc aatcgtgtca acaaagccga
     1441 agccggacac tttgcaaaag caggtgttga agccggtcgc ggtttgattg agtttgcttt
     1501 gactgaagaa aaattagctg aattgaaagc cggtgacgaa atcaccgttt ctatgtttga
     1561 agttggtcaa ctggtcgatg taaccggtac ctctaaaggt aaaggtttct ccggtacgat
     1621 caaacgtcat aacttcggcg cccagcgtac ttcccacggt aactcccgtt ctcaccgtgt
     1681 tccaggctct atcggtatgg cgcaagaccc gggtcgcgtg ttccccggta aacgcatggc
     1741 cggccaatac ggcaacacca aagcaactgt tcaaaaattg gaagttgtcc gtgttgacgc
     1801 agaacgccaa ctgctgttgg ttaagggtgc tgttccaggt gcggtcaaca gcgatgttgt
     1861 agttcgtccc agcgtgaaag taggtgcgta atggaattga aagtaattga cgctaaagga
     1921 caagtttcag gcagtctgtc tgtttctgat gctttgttcg cccgcgaata caatgaagcg
     1981 ttggttcatc agctggtaaa tgcctacttg gcaaacgccc gctctggtaa ccgtgctcaa
     2041 aagacccgtg ccgaagtaaa acactcaacc aaaaaaccat ggcgtcaaaa aggtaccggt
     2101 cgcgcccgtt ccggtatgac ttcttctccg ctgtggcgta aaggcggtcg cgcgttcccg
     2161 aacaaacccg acgaaaactt cactcaaaaa gtaaaccgca aaatgtaccg tgccggtatg
     2221 gcgactattc tgtcccaatt ggctcgtgac gagcgtttgt ttgcgattga ggcgttgact
     2281 gccgaaactc ctaaaaccaa ggtttttgct gaacaagtga aaaatctagg tctggagcaa
     2341 gtgttgtttg taaccaaaca gctcgacgag aatgtttact tggcttcacg caacttgcca
     2401 aacgtgttgg ttttggaagc tcaacaagtt gatccttata gcttgctgcg ttacaaaaaa
     2461 gtaatcatca ctaaagatgc agttgcacaa ttagaggagc aatgggtatg aatcaacaac
     2521 gtttgactca agtgattttg gcacctatcg tttctgaaaa aagcaacgta ttggctgaaa
     2581 aacgcaacca aatgacgttt aaggttttgg caaatgcaac caaacctgaa atcaaagctg
     2641 ctgttgagct gctgtttggt gttcaagttg cttctgtaac taccgttaca actaaaggca
     2701 aaactaagcg ttttggtcgt actttgggcc gccgcagcga tgttaaaaaa gcttatgtaa
     2761 gcttggctgc cggtcaagag ttggatttgg aagccgctgc tgcagctgca gataaggaat
     2821 aaacaaaatg gcaatcgtta aaatgaagcc gacctctgca ggccgtcgcg gcatggttcg
     2881 cgtggtaaca gaaggtttgc acaaaggtgc accttatgcg cctttgcttg aaaagaaaaa
     2941 ttctactgcc ggccgtaaca ataatggtca tatcaccacc cgtcacaaag gcggcggtca
     3001 taaacaccat taccgtgttg tagactttaa acgtaacaaa gacggcattc ctgccaaagt
     3061 agagcgtatc gaatacgatc ctaaccgtac tgccttcatc gcactgttgt gctatgcaga
     3121 cggcgagcgt cgttacatca tcgctcctcg cggtattcaa gtcggtgccg tattggtttc
     3181 cggtgctgaa gctgccatca aagtaggcaa caccctgccg atccgcaaca ttcccgttgg
     3241 tacgactatc cactgtatcg aaatgaaacc cggtaaaggt gctcaaatcg cacgttctgc
     3301 cggtgcttct gcggtattgc tggctaaaga aggcgcgtac gctcaagtcc gcctgcgctc
     3361 tggcgaagtc cgtaaaatca acgtagattg ccgtgcaacc atcggtgaag tcggtaacga
     3421 agagcaaagc ctgaaaaaaa tcggtaaagc cggtgccaat cgttggcgcg gtattcgtcc
     3481 gactgtacgt ggtgttgtca tgaaccctgt cgatcacccg catggtggtg gtgaaggccg
     3541 tacgggcgag gcccgcgaac cggtcagccc atggggtact cctgctaaag gctaccgcac
     3601 tcgtaataac aaacgcacgg ataacatgat tgttcgtcgc cgttactcaa ataaaggtta
     3661 atttagtatg gctcgttcat tgaaaaaagg cccatatgta gacctgcatt tgctgaaaaa
     3721 agtagatgct gctcgcgcaa gcaacgacaa acgcccgatt aaaacctggt ctcgtcgttc
     3781 taccattctg cctgatttta tcggtctgac cattgctgtg cacaacggcc gcacccatgt
     3841 gcctgtgttt atcagcgaca atatggttgg tcataaatta ggcgaattct cattgacccg
     3901 tacctttaaa ggccacttgg ccgataaaaa ggctaaaaag aaataaggtg aatcatgaga
     3961 gtaaatgcac aacataaaaa tgcccgtatc tctgctcaaa aggctcgttt ggtagctgat
     4021 ttgattcgtg gtaaagacgt tgcccaagct ttgaatattt tggctttcag tcctaaaaaa
     4081 ggtgccgagc tgattaaaaa agtattggag tcagctattg ctaatgccga gcacaataac
     4141 ggtgcggaca ttgatgaact gaaagtggta actatctttg ttgacaaagg cccaagcttg
     4201 aaacgttttc aagctcgcgc caaaggtcgc ggtaaccgca tcgaaaaaca aacttgtcat
     4261 atcaatgtga cagtgggtaa ctaaggaaaa gctatgggac aaaagattaa ccctacaggc
     4321 tttcgcctgg cggtaactaa agactgggct tcaaaatggt ttgctaaaag caccgacttt
     4381 tctactgttt tgaagcagga tatcgatgtt cgcaattatt tgcgtcaaaa attggccaat
     4441 gcttcggttg gtcgagtggt tattgaacgc cctgcaaaat ctgcacgcat taccattcac
     4501 tccgctcgtc cgggtgtggt tatcggtaaa aaaggtgagg atatcgaggt tttgaaacgt
     4561 gacttgcaag tcttgatggg tgtacctgtt catgtaaata ttgaagagat tcgccgtcct
     4621 gagttggatg ctcaaattat tgctgacggt attgcccagc agttggaaaa gcgcgttcaa
     4681 ttccgtcgtg ctatgaaacg agcaatgcaa aatgcaatgc gttctggtgc taaaggcatt
     4741 aagattatga cttcaggccg tctgaatggt gcggatattg cccgtagtga atggtatcgt
     4801 gaaggtcgcg tgccactgca tactttacgt gcaaatgtag attatgcaac cagcgaagcg
     4861 cacaccacat atggtgtatt gggtctgaaa gtttgggttt atacggaagg caatattaaa
     4921 tcttccaaac ctgaacatga gagtaaacaa agaaaggcag gtagacgtaa tgctgcagcc
     4981 aactagactg aaataccgta agcaacaaaa gggtcgcaat actggcatcg ctactcgcgg
     5041 taataaggta agtttcggtg agttcggctt gaaagccgta ggtcgtggtc gtttgactgc
     5101 ccgtcaaatc gaagctgctc gtcgtgcaat gacccgtcat atcaaacgtg gtggtcgtat
     5161 ttggattcgt gtattccctg ataaaccgat tactgaaaag cctattcaag ttcgtatggg
     5221 tggcggtaaa ggtaacgtgg aatattacat tgccgaaatt aaaccaggta aagtgttgta
     5281 tgaaatggat ggcgttccag aggaactggc tcgtgaagca ttcgagttgg ctgctgccaa
     5341 attgcctatt cctacaacct ttgtagtaag acaggtgggt caataatgaa agcaaatgaa
     5401 ttgaaagaca aatccgttga gcagttgaat gcagatttgt tggacttgtt gaaagctcag
     5461 tttggcttac gtatgcaaaa cgctaccggt caattaggca aaccaagtga attgaaacgt
     5521 gtacgtcgcg atattgctcg tattaaaacc gttttaactg aaaaaggtgc taagtaatga
     5581 gcgaaactaa aaatgttcgt actttgcaag gcaaagtagt aagcgacaaa atggataaaa
     5641 ccgtaacagt attggttgag cgtaaagtaa aacatccgct gtatggtaag attattcggt
     5701 tatctactaa aatccatgcc catgatgaaa ataatcaata tggaattggt gatgtggttg
     5761 ttatatcgga atcccgtcca ttgtcaaaaa ctaaatcttg ggttgtcagt gagctggttg
     5821 agaaagcacg ttctatttaa gaattaaagc aacgtgcttg gaatgggaaa cgaagtattg
     5881 cagcaaattt aatttgcgtg taaacttcgt ttcctgtctt tcagtttctt ctggaagttt
     5941 cttccctttt cggggtccaa gactggttta cttgaaccgc aaggtttcat ttaataagca
     6001 gcggctttgc tgtaagttat ctgaaagtgg taaattaagt tggttaattt aaaggtaata
     6061 acatgattca aatgcagacc atcttagatg tggctgataa ctctggtgcg cgtcgcgtaa
     6121 tgtgtatcaa ggtattgggc ggatctaagc gtcgctacgc ttctgttggc gatattatta
     6181 aagtggcagt taaagatgcg gctccgcgtg gccgtgtcaa aaaaggcgat gtatataatg
     6241 cggtagttgt tcgtactgct aagggtgtac gtcgtcctga tggtgcgtta attaaattcg
     6301 ataacaatgc cgccgtgtta ctgaataata aacttgaacc tttgggtact cgtatctttg
     6361 gtccggtaac ccgtgaattg cgtactgagc gatttatgaa aatcgtttca ttggcacctg
     6421 aagtattata aggaatggca cgatgaataa aatcattaaa ggcgataggg ttgtagtaat
     6481 tgctggtaag gataaaggta agcagggtca agtagttcga gtgttgggtg ataaagttgt
     6541 tgttgagggc gttaatgttg taaaacgcca tcaaaaacct aatccaatgc gtggcattga
     6601 gggcggtatt attactaaag aaatgccttt ggatatttct aatatcgcaa tcctgaatcc
     6661 ggaaactaat aaagcggacc gtgttggtat taagctgatt gaaaatgaag gcaaagttaa
     6721 acgcgttcgt ttcttcaaat caaatggctc tatcattggg gcataaggag ataacatggc
     6781 tcggttgaga gagttttata aagagacagt tgttcctgaa ttggttaaac aatttggtta
     6841 caaatcagta atggaagtcc cgcgtattga aaaaattacc ttgaatatgg gtgtgggtga
     6901 ggctgttgct gataaaaaag ttatggaaca tgctgtttcc gatttagaga aaattgccgg
     6961 tcaaaaaccg gttgttactg ttgcccgtaa atctatcgca ggttttaaaa tccgtgataa
     7021 ctatccggtt ggttgcaaag taacattgcg tcgtgatcaa atgtttgaat tcttggatcg
     7081 tttgattact attgcattac ctcgcgtacg tgacttccgt ggtgtgagcg gtaaatcatt
     7141 tgatggccgt ggcaattaca atatgggtgt tcgtgagcaa attatttttc cggaaattga
     7201 atacgataaa attgatgctt tgcgtggttt gaatattact attactacta cagcaaaaac
     7261 cgatgaggaa gcgaaagctt tattgtcatt gtttaaattt ccgttcaaag gataatcatg
     7321 gctaagaaag cacttattaa tcgtgatctg aaacgtcaag ctttggctaa aaaatatgcg
     7381 gctaaacgcg cggcaattaa agcggtaatc aatgattcga atgcaactga ggaagagcgt
     7441 tttgaggctc gtttgaggtt tcaatccatt cctcgtaatg cggcacctgt gcgtcaacgt
     7501 cgtcgttgtg ctttgacagg tcgccctcgt ggtactttcc gtaaatttgg tttgggtcgt
     7561 attaaaatcc gtgaaatcgc catgcgtggc gaaattccgg gtgttgttaa agccagctgg
     7621 taataggagt aattaagaat gagtatgcat gatcctattt ccgatatgtt gactcgtatc
     7681 cgcaatgcgc aacgtgctaa taaagcagcg gttgcaatgc cttcttcaaa attaaagtgt
     7741 gctattgcaa aggtattgaa agaagaagga tatattgagg acttcgcagt ttcatctgac
     7801 gtaaagtcta tattggaaat tcaattaaaa tactatgcag gtcgtcctgt aattgaacaa
     7861 atcaagcgtg tatctcgccc tggtttgcgt atttataaag cgtctagtga gattccaagt
     7921 gttatgaatg gcttgggtat tgctattgtt agtacttcta aaggtgtaat gactgatcgt
     7981 aaagcacgtt ctcaaggtgt tggtggtgag ttgttatgca ttgtagccta gtggaggaaa
     8041 agaaatgtca cgtgtcgcaa aaaacccagt gactgttccc gctggtgtag aagtaaaatt
     8101 tggaacagag gcattagtta ttaagggtaa gaacggtgaa ttgtcttttc ctttgcattc
     8161 tgatgtagcc attgaattta atgatggcaa attgactttt gttgcgaata acagcagtaa
     8221 acaagcaaat gcaatgtctg gtactgctcg cgcattagtc agcaatatgg ttaaaggtgt
     8281 ttcagaaggt tttgagaaaa gattgcaatt gataggtgtg ggttatcgtg ctcaagcaca
     8341 aggtaaaatc ttgaatctgt ctttgggttt ttctcatccg atcgtatatg aaatgcctga
     8401 aggtgtctcc gttcaaactc ctagccaaac agagattgtt ttaaccggct cggataaaca
     8461 agttgttggt caagttgctg ctgagattcg tacgttccgt gctcctgagc cttataaagg
     8521 taaaggtgtt cgctatgtag gcgaagtagt ggtaatgaaa gaagccaaga aaaaataatt
     8581 gaggttcact aatggataaa catacaaccc gactccgtcg tgcacgcaaa acccgtgctc
     8641 gtattgcgga cttgaaaatg gtaagattat gtgtgttccg aagcaataat catatttatg
     8701 ctcaagtaat tagtgctgaa ggtgataaag tattggctca agcctctaca ttggaagctg
     8761 aggtgcgcgg tagtctgaaa tctggaagca atgttgaagc agctgcaata gttggtaaac
     8821 gtattgctga gaaggctaaa gcagcaggtg tagaaaaggt tgcttttgat cgttcaggtt
     8881 tccaatatca cggtcgtgtg aaggctttgg ctgaagctgc tcgtgaaaat ggtttaagct
     8941 tctaaatatt tggagacttt cagatggcaa aacatgaaat tgaagaacgc ggtgacggtc
     9001 tgattgaaaa gatggtcgca gttaaccgtg taaccaaagt agttaaaggt ggtcgcatta
     9061 tggctttctc tgcgctaact gttgttggtg atggagatgg tcgtattggt atgggtaaag
     9121 gtaagtcaaa agaagtgcct gttgcagtcc aaaaggcgat ggatcaagca cgacgctcta
     9181 tgattaaggt accattaaaa aatggtacga ttcatcatga ggttattggt cgacatggtg
     9241 ctactaaagt atttatgcag cctgctaaag agggtagtgg cgtaaaagcc ggtggaccta
     9301 tgcgtttggt ttttgatgct atgggcattc ataatatctc cgccaaagtg cacggatcta
     9361 ctaacccata taatatcgta cgtgcaacat tagatggttt gtctaagttg catactcctg
     9421 ctgatatcgc agccaaacgt ggcttgacag tggaagacat tttgggagtt aaccatggct
     9481 gaacaaaaaa agattagggt tacattggtt aaaagcctga ttggtacaat tgaatctcat
     9541 cgtgcatgtg cacgcggttt aggtttgtgt cgtcgcgagc atacggtaga ggttttagat
     9601 acccctgaaa accgtggtat gattaataaa atcagctact tgttgaaagt ggagtcttga
     9661 tatgtttttg aatacaattc aacctgctgt tggtgctacg catgctggtc gtcgtgttgg
     9721 acgcggtatt ggtagtggtc ttggcaaaac gggtggtcgt ggtcataaag gtcaaaagag
     9781 ccggtctggt gggtttcata aggtgggttt cgagggtggt caaatgccct tgcaacgacg
     9841 cctccctaaa agaggtttta aatctttaac agcatcagct aatgcacagc ttcgtttaag
     9901 tgaactggaa tcaattgctg ttaatgagat tgatattttg gtcttaaagc aagcgggtct
     9961 gattgcatct acagtctcta atgttaaagt tattgcttct ggtgaaattt ctaaggcagt
    10021 tgctttgaag ggtattaaag ttaccaaagg tgcgagagct gctatcgagg ctgttggtgg
    10081 taagattgaa atgtaaggtt taatattgtg gctaatcaac aaacgtcatc aggttcatcc
    10141 aaatttggag atcttaagaa acgtcttttg tttctatttg gagcattgat tgtttttcga
    10201 attggtgccc atatacccgt acctggagtt gatgctgttg ctttagctaa attatacgaa
    10261 agcgctggaa acggcatcct gggaatattg aatatgtttt ccggtgggtc gttagagcgc
    10321 tttagtatat ttgcaatagg aattatgcca tatatttcag cttctattat tgtacagctc
    10381 gcttctgaaa ttttgccatc attgaaggct ttaaaaaaag aaggggaggc tggtagaaag
    10441 gtaattacga aatatactag gtatggtact gttttgttag caattcttca aagtctaggt
    10501 gttgcatctt tcgtatttca gcaaggaatt gttgtaacaa gttcatttga gtttcatgtt
    10561 tccacggtag tttctttggt aacgggaacc atgtttctta tgtggcttgg ggagcaaatt
    10621 actgaaaggg gtatcgggaa cggtatttct ttaatcatta cggcaggtat tgcttcaggt
    10681 attccttcgg gtattgcaaa gctggttaca ctgacgaacc aaggttctat gagcatgctt
    10741 acggcgttgt ttattgtatt tggtgcctta ttattaattt atttggttgt atactttgaa
    10801 agtgcacagc ggaagattcc tattcattat gcaaaacgcc agtttaatgg tagggcgggt
    10861 agtcaaaata cgcatatgcc tttcaagttg aatatggctg gtgttattcc cccaattttt
    10921 gcttccagta ttattctatt tccatctact cttttaggtt ggtttggttc ggctgataca
    10981 aatagtgttt tgcacaaaat agctggattg ttacaacacg gtcaattgct gtatatggct
    11041 ttatttgcag cgacagttat tttcttttgt tatttttata cggctttggt ttttagccct
    11101 aaagaaatgg cagagaattt aaaaaagagt ggtgcttttg ttcctgggat tagacctggt
    11161 gagcagacct ctaggtattt agaaaaagtt gtattacgtt tgacattgtt tggagctctt
    11221 tatattacaa ctatttgttt aattccagag ttcttaacta cggttttaaa tgtacctttt
    11281 tatttgggtg gcacgtcttt gttgattcta gttgttgtaa cgatggattt tagtacacaa
    11341 ataaattcgt ataggcttac tcaacagtat gataagttaa tgactcgttc agaaatgaaa
    11401 tcattttctc ggaaatagaa ttatggcgaa agaagatact atccaaatgc aaggtgaaat
    11461 tcttgaaact ttacctaatg caacatttaa agtaaaactt gagaatgacc atattgtatt
    11521 gggtcatatt tctgggaaga tgcggatgca ttacattcgt atttctccgg gagataaggt
    11581 cacagtagag ctgacacctt atgatctaac tagggctcga atcgttttca gagcaagata
    11641 aaccaataaa aggaaaataa aatgcgtgta caaccatctg ttaagaaaat ttgccgaaat
    11701 tgcaagatta ttcgtcgaaa tcgtgtagtt cgtgtaattt gtactgatct ccgtcacaaa
    11761 cagcgtcaag gttaatggaa tatttctttt aatgtgattc tgtgatatag tgacacactt
    11821 tgccctaaaa aggaaaaaat atggctcgta ttgcaggggt aaatatccct aataacgcac
    11881 acatcgtaat tggtcttcag gctatttacg gtattggtgc tactcgtgct aaattgattt
    11941 gtgaggctgc aaatattgcg cctgatacta aagcaaaaga tttggacgag actcaattag
    12001 atgctttgcg tgaccaagtt gccaagtatg aagtagaagg tgatttgcgt cgtgaggtaa
    12061 ctatgagtat caagcgattg atggacatgg gctgctatcg tggcttccgt catcgtcgcg
    12121 gcttaccatg ccgcggtcaa cgcactcgta caaatgcgcg tacccgcaaa ggtccgcgta
    12181 aagcgattgc tggtaagaaa taaattttaa ggaattttat taatggctaa agcaaacaca
    12241 gcttcacgtg tacgtaaaaa agtacgtaaa accgtgagtg agggtattgt gcacgttcat
    12301 gcatctttca acaataccat cattacaatc actgaccgtc aaggcaatgc gttgtcttgg
    12361 gctacctctg gcggcgctgg ttttaaaggt tctcgtaaaa gtacaccatt tgcagcacaa
    12421 gttgcagcag aagcagctgg taaagttgcc caagagtatg gcgttaaaaa tttagaggtt
    12481 cgtattaaag gtccaggtcc aggtcgtgaa tcctctgtac gtgctttgaa tgctcttggt
    12541 ttcaagatta ccagcattac tgacgttacc ccgttgcctc ataacggttg ccgtccgcct
    12601 aaaaaacgtc gtatttaata ttggagtgat ttgaaacatg gcacgttata ttggccctaa
    12661 atgtaagttg gcacgtcgcg aaggtacgga tttgtttttg aagagtgcgc gccgctcttt
    12721 ggattccaaa tgtaaaattg attccgctcc cggtcagcat ggtgcaaaaa aaccgcgttt
    12781 gtcagactat ggtttgcagt tgcgtgaaaa acaaaaaatc cgccgtattt atggcgtatt
    12841 agaacgtcag ttccgtcgtt atttcgcaga agctgatcgt cgtaaaggtt ctaccggcga
    12901 gttgctgttg cagttgctgg aatctcgttt ggataatgtc gtttatcgta tgggtttcgg
    12961 ttctacccga gctgaagcaa gacagcttgt ttctcataag gcgatagttg tgaatggaca
    13021 agttgtcaat attccttctt tccaagtgaa agctggtgat gttgtctcag ttcgtgaaaa
    13081 agccaaaaaa caggtacgta ttcaagaagc attgggtttg gcaactcaaa tcggcttgcc
    13141 gggttgggtt tctgtagatg cggataaact tgagggtgtg ttcaaaaaca tgccggatcg
    13201 ctcggaattg accggtgata ttaatgaaca gctggtggta gagttctact ctaaataatg
    13261 ctagctcagt gagggacagt taaatgcaga atagcacaac cgaatttttg aaacctcgtc
    13321 aaattgatgt aaatactttt tctgcaactc gtgcaaaagt atctatgcag ccatttgaac
    13381 gtggtttcgg tcatacctta ggtaatgctt tgcgccgtat cttactgtca tccatgaatg
    13441 gttttgctcc tactgaagta gctattgccg gtgtattaca cgaatattct actgttgatg
    13501 gtattcagga agatgttgtt gacattttgc tgaatattaa aggtattgtg tttaaactcc
    13561 atggtcgtag ccaagttcaa cttgtgttga agaaatcagg ttcaggtgtc gtatctgccg
    13621 gtgatattga gttgccgcat gatgtagaaa ttctgaatcc tggtcatgtc atttgtcatt
    13681 tggctgataa cggtcaaatt gagatggaaa ttaaagtaga gcaaggtcgt ggttatcaat
    13741 ctgtttcagg tcgtcaggta gttcgtgatg agaaccgtca gattggtgca atccagttgg
    13801 atgcgagctt ttcgcccatc agccgtgtta gctttgaggt tgaacctgca cgtgtagagc
    13861 agcggacgga tcttgataag ttggttttgg atatcgaaac cgacggttct attgatcctg
    13921 aggaagctgt acgcagtgcg gcacgtattt tgattgatca gatgtctatt tttgctgatt
    13981 tgcagggtac tcctgtggag gaggttgaag aaaaagcacc tcctatcgac cctgttcttt
    14041 tgcgtccggt ggatgatctg gaattgacag tacgttcagc taattgtttg aaagctgagg
    14101 atatttatta tattggcgat ttgattcaac gcactgaaac cgagcttctt aaaacgccga
    14161 atttgggacg taaatctttg aatgagatta aggaagtatt ggcatctaaa ggtttgacac
    14221 tgggttctaa gttggaagca tggccacctg taggcttgga aaagccttaa tgaagaatta
    14281 aaggataatt gatatgcgtc atcgtaatgg caatcgcaaa ttaaaccgta ccagcagtca
    14341 tcgtgctgca atgctgcgta atatggcgaa ttcattattg actcacgaaa ctattgtaac
    14401 aactctgcct aaggccaagg aattgcgccg tgtagtagag ccgttgatta cattgggtaa
    14461 aaagccgtca ttggcaaacc gccgtttggc atttgaccgt actcgcgacc gtgatgttgt
    14521 agtaaaactg tttggcgatt tgggtcctcg ttttactgct cgtaacggtg gttatgttcg
    14581 ggtgttgaaa tacggattcc gtaaaggtga taatgcacct ctggcactgg ttgaattggt
    14641 tgacaaaccg gctgctgagt aattttagtc atataacgcc atctgccgaa aagcaggtgg
    14701 cgttattttt gcaatatctg ataggtaata gggtattggc tatcatgttt aaaatattaa
    14761 ttgaatagct aaggtttgcg cggtaaactt acatcattaa aaaattctat gatggtttat
    14821 ataatgaatg ctttcgatat aaagtcgaca aagatggacg tattgtctat atctttgcat
    14881 acgtcagact tgtttgattt ggaagatgtg ctggtcaaat tgggcaagaa gtttcaagag
    14941 tctggtgttg ttccatttgt gctggatgtt caagagtttg attatcccga gtctttggat
    15001 cttgctgcat tggtttcgtt gttttcaagg catggtatgc aaattttggg tctgaagcat
    15061 tctaatgaac gttgggctgc tgcggctatg aagtatcatt tgctgttttg tctgtctcat
    15121 tcggaaaatg ttaaagaact gggtcaggtt gaggtgcaga acacggagga tggtcagaaa
    15181 gcaaggaaaa cagtattgat tacatcccct gtccgtaccg gtcagcaggt ttatgccgaa
    15241 gatggcgatt tgattgttac gggggcggtc agccaggggg cggaattgat tgcagatggc
    15301 aatatgcata tttatgcgcc gatgagaggg cgtgctttgg ccggtgccaa aggcgatact
    15361 tctgcccgca tatttatcca ctccatgcag gcagaactgg tttctgtggc gggtatttac
    15421 cgtaattttg aacaggattt gccggaccat ctgcacaagc agccggtaca gatattgttg
    15481 caggataacc gattggttat cagtgcaatt ggctcagaat aattgtttga tattttaaaa
    15541 aggaaatatt gtggcaaaaa ttattgtagt aacttcaggt aagggcggtg tcggtaaaac
    15601 gactaccagt gccagtattg cgacaggttt ggcattacgc ggatataaaa ctgcggtaat
    15661 tgattttgat gtgggtttgc gtaacctcga cctcattatg ggttgcgagc gtcgtgtcgt
    15721 ttatgacctg atcaatgtca ttcaggggga ggcgacgctc aaccaggctt tgattaaaga
    15781 taaaaattgt gaaaacctgt ttattttgcc ggcttcccag actcgggata aagacgcttt
    15841 gacacgcgag ggagtagaaa aagtgatgca ggagctgtcc ggcaagaaaa tgggctttga
    15901 gtatattatt tgcgactctc ctgccggtat tgagcagggt gcattgatgg cgttgtattt
    15961 tgctgatgaa gccattgtaa cgaccaatcc tgaggtttcc agtgtgcgtg actccgacag
    16021 gattttggga attttgcaaa gcaaatccca taaggcagag caaggcggtt cggttaaaga
    16081 acatctgttg attacgcgtt attctcccga acgtgtggca aaaggcgaaa tgctgtctgt
    16141 acaggatatt tgcgatattc tgcatatccc tttgctgggt gtgattcctg aatcccaaaa
    16201 cgtcttgcag gcatccaatt ccggagaacc ggtcatccat caggacagcg tggcggcttc
    16261 cgaggcatat aaggacgtta ttgcccgtct tttgggcgag aaccgtgaaa tgcgcttctt
    16321 ggaagctgag aaaaaaagct tcttcaaacg tctgtttgga ggataaggta tgtcattaat
    16381 cgaattttta ttcggcagaa agcagaaaac ggcaaccgtt gcccgcgacc gccttcaaat
    16441 catcattgcc caagagcgcg cccaagaagg tcaggctccg gattacctgc cgactttacg
    16501 taaagagttg atggaagtcc tgtccaaata tgtgaacgtt tcattagaca atatccgtat
    16561 ttcccaggaa aaacaggatg gtatggatgt gcttgagttg aacattactt tgccggaaca
    16621 gaaaaaggta taggacatga ccttaaccga attgcggtac atcgtcgcag tcgcccaaga
    16681 acgtcatttc ggcagggcgg cgcggcgttg ttttgtcagt cagcccactt tgtctattgc
    16741 cattaagaaa ttggaagaag agcttgccgt ctctttgttt gaccggagca gcaacgatat
    16801 tattacgacc gaggcggggg aacgtatcgt tgcacaggcg cgtaaggtat tggaagaggc
    16861 ggagcttatc aggcatttgg caaatgaaga acaaaacgag ctggagggtg cgttcaaact
    16921 cgggctgatt tttacggttg cgccgtacct gctgccgaaa ctgattgtct cgttgcgccg
    16981 tactgcaccg aaaatgcctt tgatgttgga agagaattac acgcatactt tgaccgagtc
    17041 gctcaaacgc ggggacgttg atgcgattat cgttgccgaa ccgtttcaag agccgggcat
    17101 tgttaccgaa cccttgtatg acgaaccgtt tttcgtgatt gtcccgaaag ggcattcatt
    17161 tgaggaactg gatgccgttt cgccccggat gctgggtgag gagcaggttt tgctgctgac
    17221 ggaaggcaac tgtatgcggg atcaggtact ctcaagctgt tccgaattgg cggcgaaaca
    17281 acgcatacag gggttgacca atacattgca gggcagctcg atcaatacaa tccgccatat
    17341 ggtcgccagc ggtttggcaa tcagcgtgtt accggcaacc gcgctgaccg aaaacgatca
    17401 tatgctgttc agcattattc cgtttgaagg tacgccgcca agccggcggg tcgtattggc
    17461 gtaccgccgc aattttgtcc gtccgaaggc gttgtcggcg atgaaggcgg cgattatgca
    17521 gtcgcagctt cacggggtaa gttttatccg cgactaggcg caggcattgt tttcaaaacg
    17581 ccatttccct gagccgacaa cacggtatgc caagatattg ccgtcatcat cgattttgag
    17641 tatagcatcg ccacggaaac tgccgtcctg aagatattcg acttttgcat cactgtgaat
    17701 gttttcatca gtgccgatgc aatgcc
//
LOCUS       DAXHPG010000027        16987 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXHPG010000027 DAXHPG010000000
VERSION     DAXHPG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16987
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.027"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(51..1220)
                     /gene="metK"
                     /locus_tag="ACFM6F_000675"
     CDS             complement(51..1220)
                     /gene="metK"
                     /locus_tag="ACFM6F_000675"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC6164140.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1403..1513)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1566..2462
                     /locus_tag="ACFM6F_000676"
     CDS             1566..2462
                     /locus_tag="ACFM6F_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6164141.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2506..3570)
                     /gene="tsaD"
                     /locus_tag="ACFM6F_000677"
     CDS             complement(2506..3570)
                     /gene="tsaD"
                     /locus_tag="ACFM6F_000677"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC6164142.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            3577..3702
                     /locus_tag="ACFM6F_000678"
     CDS             3577..3702
                     /locus_tag="ACFM6F_000678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164143.1"
                     /translation="MPLKTEKRILADFGTTAVSQKRNMPSEDRSDGIVAPYQSVP"
     gene            complement(3687..4874)
                     /gene="ccsB"
                     /locus_tag="ACFM6F_000679"
     CDS             complement(3687..4874)
                     /gene="ccsB"
                     /locus_tag="ACFM6F_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC6164144.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4867..6819)
                     /locus_tag="ACFM6F_000680"
     CDS             complement(4867..6819)
                     /locus_tag="ACFM6F_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC6164145.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGKGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7096..7719)
                     /locus_tag="ACFM6F_000681"
     CDS             complement(7096..7719)
                     /locus_tag="ACFM6F_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6164146.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7925..8554
                     /gene="yihA"
                     /locus_tag="ACFM6F_000682"
     CDS             7925..8554
                     /gene="yihA"
                     /locus_tag="ACFM6F_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC6164147.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8675..11071)
                     /locus_tag="ACFM6F_000683"
     CDS             complement(8675..11071)
                     /locus_tag="ACFM6F_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC6164148.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11223..12338
                     /gene="pilM"
                     /locus_tag="ACFM6F_000684"
     CDS             11223..12338
                     /gene="pilM"
                     /locus_tag="ACFM6F_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC6164149.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12341..12940
                     /locus_tag="ACFM6F_000685"
     CDS             12341..12940
                     /locus_tag="ACFM6F_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC6164150.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKVEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12941..13588
                     /locus_tag="ACFM6F_000686"
     CDS             12941..13588
                     /locus_tag="ACFM6F_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC6164151.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13606..14151
                     /locus_tag="ACFM6F_000687"
     CDS             13606..14151
                     /locus_tag="ACFM6F_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC6164152.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14170..16479
                     /gene="pilQ"
                     /locus_tag="ACFM6F_000688"
     CDS             14170..16479
                     /gene="pilQ"
                     /locus_tag="ACFM6F_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC6164153.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQQ
                     AAAPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRS
                     LDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESG
                     GVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWD
                     QALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKY
                     KNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDE
                     LDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKW
                     GAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAK
                     IESGYEIPFTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQC
                     ASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFK
                     TRGKKTDRRELLIFITPRIMGTAGNSLRY"
BASE COUNT         4580 a         4822 c         3882 g         3703 t
ORIGIN      
        1 aaaatgccgt ctgaactgtt cagacggcat ttttgatttt caaaccggaa ttacagcccc
       61 gctgccgccc tcaatgcagc agctttgtcg gtgcgctccc aagtgaactc aggttcttcg
      121 cggccgaaat gtccgtaagc ggcggattta ctgtaaatcg ggcgcaagag atcgagcatt
      181 tggacgatgc ctttggggcg caggtcgaaa tgttcgcgaa ctaaggtaat cagtttttcc
      241 tcgctgattt tgcctgtgcc gaaagtgtcg atggaaatcg aagtcggttc ggcaacgccg
      301 atggcgtagg aaacttggat ttggcattgg gttgccaatc ctgcggcgac gatgtttttc
      361 gccacatagc ggcaggcgta ggcggcggaa cggtcaacct tggacgggtc tttgccggaa
      421 aacgcgccgc cgccgtgcgg agccgcgccg ccgtaggtat cgacgatgat tttacggccg
      481 gtcaaaccgc agtcgccttg cgggccgccg atgacgaagc ggccggtcgg gttgatcagg
      541 tatttggttt cgtcggtcag cagttcagac ggcaggacgg gcttgataat ctgctcgatg
      601 acggcgtttt tcagttcttc gtaagcgatg gacggatcgt gctgggtaga gaggacgacg
      661 gtatcaatgc gttttacctt accggtttcg ctgtcgtaaa ccacggtcaa ctgcgctttt
      721 gcgtcgggac gcagccacgg caggcggccg tctttacgca attcgctttg gcgttgcatc
      781 aggcggtggc tgtaatagat ggcaaacggc atcagggtcg gggtttcgtc gcaggcataa
      841 ccgaacatca aaccttggtc gcctgcgcct tggttcaagt cgataccttc gccttcgttt
      901 acgccttggg cgatgtcggg ggattgttgg tcgtagtaca cgccgaccgc gcagccgttg
      961 gcatcaaagc ccagctcgga ggagttgtag ccgatgcgtt tgatggtttc gcgtgcgact
     1021 ttgatgtagt ccacttgggc ggtggtggtg atttcgcctg ccaatacgca caagcctgtg
     1081 ttgaccaaag tttctgcggc gacacgcgct ttggggtctt gcgccaagat ggcatccaaa
     1141 atcgcatcgg atacttggtc ggcaactttg tccggatggc cttcggatac cgattcggaa
     1201 gtaaacagat attcgctcat tgagattttc cttgaaaaac aaaccatctt cttcagacgg
     1261 catgttgtat gaacataatg tcgacagcgg gaaatatagc aaaatttccc tattcatacc
     1321 attcagttga gaaatattcc catttgaatg gcactttgga atctctgccc gtacgtttct
     1381 tacaggcaaa aaaattcccg catcaagcgg gtttggattg ctctggtgag ccacatcggc
     1441 ttttcaaccg tccaccttac ttttcctttt gaaaagcagg ttggcatgga attcccaact
     1501 cttaatgcag cgcggatggt agcagaaaag cctttccgcc gcaatacttc cctttttcag
     1561 acggcatgtt tcgtttacaa ttcgggctgt ttcccccttt gcgaaccgcc atgcacatcc
     1621 tgttgaccgc cctgctcaaa tgcctctccc tgctgccact ttcctgtctg cacacgctgg
     1681 gaaaccggct cggacatctg gcgttttacc ttttaaagga agaccgcgcg cgcatcgtcg
     1741 ccaatatgcg tcaggcaggc atgaatcccg accccaaaac agtcaaagcc gtttttgcgg
     1801 aaacggcaaa aggcggtttg gaacttgccc ccgcgttttt cagaaaaccg gaagacatag
     1861 aaacaatgtt caaagcggta cacggctggg aacatgtgca gcaggctttg gacaaacacg
     1921 aagggctgct attcatcacg ccgcacatcg gcagctacga tttgggcgga cgctacatca
     1981 gccagcagct tccgttcccg ctgaccgcca tgtacaaacc gccgaaaatc aaagcgatag
     2041 acaaaatcat gcaggcgggc agggttcgcg gcaaaggaaa aaccgcgcct accagcatac
     2101 aaggggtcaa acaaatcatc aaagccctgc gttcgggcga agcaaccatc gtcctgcccg
     2161 accacgtccc ctcccctcaa gaaggcgggg aaggcgtatg ggtggatttc ttcggcaaac
     2221 ctgcctatac catgacgctg gcggcaaaat tggcacacgt caaaggcgtg aaaaccctgt
     2281 ttttctgctg cgaacgcctg cctggcggac aaggtttcga tttgcacatc cgccccgtcc
     2341 aaggggaatt gaacggcgac aaagcccatg atgccgccgt gttcaaccgc aatgccgaat
     2401 attggatacg ccgttttccg acgcagtatc tgtttatgta caaccgctac aaaatgccgt
     2461 aacgaaaata aaaatgccgt ctgaacaatt tcagacggca ttttgtcatc tgacgatttc
     2521 cgacagcggc cagcgcggac ggacattgaa cgcaccgacc tccctgccct tgcccaggtg
     2581 catcgcaccg gcaaaggcaa tcatggcacc gttgtccgtg cagtatgccg tcggcgggaa
     2641 aaacacgctg actttttcgg acggatgttt cggcttgcct tttggggtcg ggatttgcac
     2701 cgtcatattg ccgaaagttt cacggagttt gcggtttgca ccgaccccgc cggcgaccac
     2761 tacggttctg aaccctgtct gcaacagggc ttttttcact ttcgccgcca acacatcgac
     2821 taccgcatct tgaaacgcac ggcaaatatc gttgcgggtt tgctcgggaa tggtttccga
     2881 accgtttgcc tcacgcactt tttcaacggc agtcaaaaca gcggttttca aacctgaaaa
     2941 actcatctgc aaatcgtcgg aatgaatcat cgggcgcgga aaaacaaacg cttcgggcct
     3001 gcccgattcc gcaagttccg acagtttcgc accgcccgga tacggcaagc ccagcagttt
     3061 cgccgttttg tcgaatgcct cgcccgccgc atcatcgacg ctctcgccca aaagcgcgta
     3121 gtcgcctatg cccctgaccg ccataatctg cgtatgcccg cccgaaacca gcagcgcgac
     3181 aaaaggaaag tcgggttttt cctccgccaa cagcggcgac agcagatgtc cttccaaatg
     3241 atggacggga ataacaggct tgtccaacgc taaagccagc gcgttggcgt agctcgaacc
     3301 cgccagcagc gcaccgccca aaccgggccc ctgcgtaaag gcaaccgcgt caatgtcgcc
     3361 atacgatgcg cccgcctgcg ccaaacagcc ttcagtcaac ggaacaaggc ggcggatatg
     3421 gtcgcggctt gccaattccg gcacaacccc gccgtattcg gcgtgcattg ccatttgagt
     3481 gtgcaggcag tgcgcccgca atccacgttc cgtatcgtaa agcgcaacac ctgtttcgtc
     3541 gcaagaagac tcgattccta ataccaacat ggtctgatgc cgttaaaaac tgaaaaacgt
     3601 attttagcgg atttcggcac gactgccgta tcccaaaaac ggaacatgcc gtctgaagac
     3661 cgttcagacg gcatcgtcgc accgtatcaa agcgttccgt aagaatgcag cccgctcaaa
     3721 aacatattca cgccgataaa ggcaaatgcg gttacgaaca aaccgataat cgcccaccac
     3781 gccagcactt tgccgcgcca accggcaacc agccgcaagt gcagccaaac ggcgtaattg
     3841 agccagacga tgaacgccca cgtctctttc ggatcccaac tccaatagcg tccccaagca
     3901 tctgccgccc acagcgcacc caaaatggtg gcaatagtaa agaacagaaa gccgacggca
     3961 atcgccttat acatcacctc gtcgatcaat gccgacggcg gcagccacag ttttccgcct
     4021 tttccttccg cacgcaggga aaccagttcg gcaataccga gcatcgcgga aatgcaaaac
     4081 gcgccgtaac cgataaagtt tgccggaacg tggattttca tccaccagga ctggagcgcg
     4141 ggaatcagcg gctggatggt atgcgcctcg cgggacacgc tgtaccacaa gacaaatcca
     4201 accacgaccg ccataaagcc gaacacgaag ccgcccaatt tctgtatggc gaacttacct
     4261 tcataataaa gatacatcag cgcggtaatc accaaaaaca ggatgaacac ttcatacagg
     4321 ttggaaaccg gaatatgccc cgcatcggga cggagcagat agctttcgtg ccaacgtacc
     4381 agcagaccgg taaagcctgc tacggcagac acccatgcaa acacggttcc catacccaac
     4441 agcgtgttgg tcggcacatt ttttacgctt gccaaaaccg cgcccgaaat ataagcgaac
     4501 agggcgaaaa agacaaaggc gcactgccac atgatcgccg attggctgct gaggaaatac
     4561 cgcaacagga aaatctctgc cgatttgatg ttgccgccat acagaccgac ggcggcatag
     4621 gcaagcaata cgcttaaagg aacaaaccag cgcatcggtt tgaaaaacca acccaaaaac
     4681 acggcaatac cggcactcgc ccacaacatg accgtttcgt aaatgtccat atgcataccg
     4741 gaacgggtct gtacgaaaac cgtagccgca aaaaccagca cggcaaatac ccaatcccaa
     4801 agattcagat tgctgatcaa agacttctga atcagcagct cgtgttccgg aagggtttta
     4861 tagtgttcag tcatgattca agtccttgcc gagccgttgc agactctcga cgtgttttgg
     4921 aaattccttc tgcaaatccc gttcactgcg ggccgaagac atggcaaaac ggattttgcc
     4981 gtctgaaaac aatacccacg cccgtttttc gcgcacataa aacatcaata ccgtacccaa
     5041 taccaacagc accgagccga gatagaccaa aagcgcaccc ggggaacggg tcatctgcaa
     5101 acccgacgaa cgcacctcgg aaaacccatc aagttgcagc agcataggcg cgggatattc
     5161 ggtcaaaccc gtgtacgcat ccatactgtg cagcaggaaa cggttccgcg cttcatcctg
     5221 ctgccattcg ggcaagccgt accggcgtat ggtttcatcc aaagcagcgt tcatcacgcc
     5281 gtaaagcatt tcgtagaaat agccctgcat cttatcctgc tgctctttcg ggatattgga
     5341 cgtaataaat tcgtccaatc ccaaatagcc tttttgtgca aagatgttca gcgtgttttc
     5401 cgcagccagc atgaattgtt cgcggatttc ggcaggtgcg tctttggttg cgtcggcaac
     5461 cagacgtttg cgccccttcc cgtctttcag aaactcgcgc aacgccataa aggtgtccgc
     5521 tttcaactgc ttgtccaagg ggatacgcag ccagcggtat tgctgctgca agccgctgcg
     5581 cgtgccggta atccaaaaat aatcctgttc ctgcaaaacc ggcagcatat agtttttata
     5641 ttcgaccgcc tgccctgccg catcacggat acggtaaaca atggaagggc cgatattggt
     5701 gtatttttta ccttcctgag taacggcgcg gacatcgttc agcgtggatt tcaggctttt
     5761 ttcccgttcc gcgccctcgc tcatgtcctc cacattcata gaagtgaact gatcgaactc
     5821 aagacgatat ttgtgtttgc caatttccaa cggaaactgg tgtatggatg ttgccttcaa
     5881 cacgacaggc tcgcgcgaag catcacccaa attccacgcc ttgaatgtca aatccgaacc
     5941 gccgtcggca aaactcgcct gataaatcgt gatgccgtgc aaggtcaaag gatggttcac
     6001 gcggatggtg cgctcgagtt tctcaccggt tgccttgtcc gtcacttcaa tatcactggc
     6061 aaaatcgcgc ggcataccgg tattgtaaaa gtcgatatga aattttttca gtttgacttc
     6121 aaaaggcaag tcctgaacca atatcccgtt gtcggcattc aggaaaacca catccgcact
     6181 ctgcccctcg gaaatattga cgttgcccct aaatgagaga ttggacgcac ccaaaatact
     6241 ttcgggcttg aaatccttgg cataaaccgc ctgattgtcc ggaacaatcc gaccggtcag
     6301 catacccagt ttcaacagca ggttactgtc tatcaacccg cccaggcaaa tgacaatcaa
     6361 agcaacatgg gcaaagatat agccccattt gttcattgtg ccttttttgg cggcaatcag
     6421 aaccgacccg tcttcacggt taatggtttt tccctgaaaa ccttgtactt ccagataacg
     6481 tttggcaacc tcgggcgcaa tttttacatc caacagcgaa gaatggcgca tcgccgccag
     6541 agatttttct ttaacctttt cccgaaaaga cttcatttcg cgccagaacg gcggcacatt
     6601 gcgaatcagg cacaaactgg tagaaaccac caaaaacatc atgataacga caaaccatgc
     6661 cgaagcatag acgtcataca gtcccagaaa accaaaaatc tgcgcccaaa acgatccgaa
     6721 tttgaccaaa taatccgtct gcggctggtt ttgctgcaac accgtaccga taaccgatgc
     6781 aatacccagc agactgagca aagcgactgc aaagcgcatg gagctgaaaa aagcgaacca
     6841 cggacgggaa agaagtgggg gagatctacg ggatttactc attgtgtgtt tattccgcca
     6901 tcaggaatat gggaaagcag aattgggcaa acagaaaaca acgtcccgat tctactgtct
     6961 tgatgctttt tatttcaaga caatgaagac agcctgcatc gattccaacg gttgcgattg
     7021 aaaaaactta tcgcagaatt gcctgaagcc gtctgaaaac ttttcagacg gcctctaaaa
     7081 cagactattg cggaattaac gcaaaccttg gataaagttg gcgaccgctt tcaaatcttc
     7141 ttcagacata cggtttgcaa tatcttccat gatggtattt ttacgctgac cggacttgta
     7201 ggcattcatc tgttcaacaa tatatgcctg atgctgaccg cccaaacgcg gataagcctg
     7261 aatttcgctt ccgcctcccg gcatacccgc accgctcgga ccgtggcagg acatacacgc
     7321 cggcactttt ttatcgctca aaccgccgcg atagattttc gcacccaatt cgggattttc
     7381 cttaggattg gcttcaccgg atttgggctg ctgtttggca tagaatgcgg atacgttcaa
     7441 aatatcctga tcgctcaaat tcattaccac cggtttcatc acagctgccg aaccgtgggt
     7501 gcgtttaccg tcgcggatgc cgatagtttg atgatagatg taagcagtat gctgtgccgc
     7561 caaacgcgga tacatcgcaa tgccgctgtt accgtctgct gcatggcaag ccgcacaaac
     7621 cgttgcggca acctgtttgc ctttttccac gtctgctttg ggagaggcgg aaaccgcacc
     7681 ggcagccaaa acaaaggcca ataaagtcaa tcgtttcatg gagtgctcct gattacagca
     7741 ttggataacg caacaatgct ctttttatat tcaaatacgg gatttttgac ccgattaaaa
     7801 ccgatgattc tgtaaacgtg ttattctata ctaaatttac attaaattac cactgtgttt
     7861 cacataaaac caaccgcata tttttgctgt cggacaaacg gcggcggaaa acaaggatat
     7921 gcccatgaac ctttttcaaa acgccaaatt cttcacgacg gtcaaccacc ttaaagacct
     7981 gcccgacacc cctctcgaaa ttgcctttgt cggcaggagc aatgccggaa aatccagtgc
     8041 catcaatacc ctgaccaacc atgtccgtct tgcctacgtt tcaaaaacac ccggacggac
     8101 gcagcatatc aacttcttcg agctgcagaa cggcaatttt atggtcgatt tgcccggcta
     8161 cggttatgcc caagtccccg aagcagtacg cgcacattgg gtcaatctgc tcggcgacta
     8221 tctgcaacag cgcaaacagc ttatcgggct ggttttgatt atggatgccc gccatccttt
     8281 aaaagaactc gacatccgta tgctggactt tttccacacg accggcagac cggttcacat
     8341 cctgctgtca aaagccgaca aattatccaa aaacgaacag ataaaaaccc tgtcccaagt
     8401 caaaaaactg ctcaaacctt attccgacag gcaaaacatc agcgtacagc tgttttccag
     8461 cctgaaaaaa caaggtattg acgaggccaa ccgaactgtc ggaagctggt ttgacgcagc
     8521 agatgccgcc gcttcctctc cagaggaaaa ctgaccccaa ttatacggaa accgtatttc
     8581 ccccacttga ccgaccgcaa acatttaaaa aattgccact gccaaatcta aaatgccgtc
     8641 tgaaagactt ttcagacggc attttgcgga gtctttaaaa cagagaatcc aactgctgct
     8701 gtttggaacc agtattactc ggaagcaccg gcgtttcctg catatcttgg cggacttcgt
     8761 catccgccgc ctgccgtccg ccttctgccg cgcccccgtc atcttctttt gcccgtcggg
     8821 aaggttgcgg cgcaataccg ctgttgtcca gcgtcaagcc cggatcggtt accatacgtt
     8881 ccttcatata gtattcgcca ttgctgctga ccacaccttc aggcattttc atccccttgc
     8941 cctgctttcc tttcaacgca aaacgcatat agtccaccca aaccggcacc gcaatcgtac
     9001 cgccgtagcc gacacgcccc atactcttag gtttgtcgaa gccgatatat acggcagtaa
     9061 ccacatcagg gttaaaaccg acaaaccacg catccttatt gtcattggtc gtacccgttt
     9121 taccggcaat atccgttctt cccaacgcag ctgcccccct tgccgtacca acacggacca
     9181 catcctgcat aatcttatac ataatatagg cattgcgcgg atcgattgcc tgaggcgcat
     9241 tttgcccggc caccaaaggt tgcatttggg cgcgcaacct gccgtctctg tcataaatct
     9301 tatcgattac gtgcgaagaa accctatatc cgccgttcgc aaatacgcta tatgcctccg
     9361 ccactttcaa cggcgttgtc tcgcccgtac ctaaagccat agacaggctt gccggcagct
     9421 cggacgacct gaagccgaaa cgccggatat actgttgcgc gtaaccgaca ccgatagaca
     9481 tcaaaatacg gatggaaacc atattcttgg aagccgtcag agcctgtctc aaagtaatgt
     9541 agccggaata tctgccgtct gaatttttag gtgtccaaac cgaaccgttc ggccctttcc
     9601 ccggcaggga aatcggcgca tcgttaacca ctgtggacgc ggtcatcccc ttagataatg
     9661 ccgccgaata gacaaacggc ttaaaggtcg aacccggctg ccgcattgcc tgaacggcac
     9721 gattgaatgt tttgctgtga aaatcataac cgccgaccag cgcgcgcaca gctccggttt
     9781 ttgcatccag cgaaaccaaa gccccctgca gcaacggctc ttgaaccacc gcccaacgcc
     9841 cgccgttgtt tttgacacgg atgaccgcgc ccctgcggat acggtcctcc cccatttttt
     9901 cattattgac cgcgcgggcc gcaaaaccca aggcgcgcct gtcaagcgta acccgcctgc
     9961 cgccgggcag ctgtatgacg acatttttct ttttagtcac atccaacaca acggcgggaa
    10021 ccattttatc gacggtatag agtcccgaca gatactggct gacagtctcc tcgacatctt
    10081 cactcttact caaatcgata tagttttccg caccgcggta gctgctgccg cgatcgaaat
    10141 tccgtagagc cttgcgcaat gcctcggttg ccaccttctg atgatcggcg cggaccgtgg
    10201 tataaacctt aaaaccctgc gtataggcat cttcaccgta tttctcatac agttcctgac
    10261 gcaccatttc cgccacatat aaagcactct gatcgatttt ccgaacaaac cgctcgtaat
    10321 gcagttcctc attcaacgcc tgatcgcgct gttgcacggt aatcatcttc tcctcgagca
    10381 tattgttcaa aatatacttc tggcgcaact tggcacgttc cggattaaca atcggattat
    10441 aggcagacgg agccttgggc agtcccgcaa gcatggcggc ttccgccaaa gtcaaatctc
    10501 ggacattctt attgaaatag atttgcgcgg cagatgcaaa accataggcg cgctgaccga
    10561 ggtaaatctg attgaaatac aactcgagga ttttgtcttt gcttaaagac tgctcgattt
    10621 tataggcaag caacacctca ttgaatttgc gtgtgaacgt tttttcactg ctcaaataaa
    10681 aatttttcgc cacctgctgc gtaatcgtac tcgcacccga ctgcacgctg ccggacacga
    10741 cattgccgac ggcagcgcgg gcaacacccc aaacatccac cccccaatgc cggtaaaagc
    10801 gtttatcctc ggcggcgata accgcattcc gcaacacctc tgggaaatcg ccgatttttg
    10861 taaattcgcg ccgctgctcc ccatacatac cgatgacttc cccatccgcc gaataaatag
    10921 tcaacggcat tttaggctgg taatgctgca aagaatccaa agacggtagt ttcggatacg
    10981 ttaccaaaat agcaatggca accaaaccca ctccaaatac acaaaaccca aaaaccaaac
    11041 caaaacaagt cgttaaaatc tttttaatca tagctgaata ataatttacc attattggta
    11101 ttaaataaag taaaatagca accgatttct acaaagcacg gtttcaatgt gcaaagaaca
    11161 aggaatccat tacggatacc gaaacggtta ctcactgtac aaataaagca ggaactttca
    11221 tcatgcgctt gtttaaaagc ttgaaaaacc ctaaaaaaac agatgccaag ctccctaaaa
    11281 aatcttcggg actcaataac cgcgcggcaa tcggcatcga tatcgaccag cattccatca
    11341 aaatggtcca attgtcagga cgtagtttaa accaaattca attggaaaaa tacgtcattg
    11401 ccaaattacc aaagaatatc attcaaggca ataaagtcca aaattacgat caacttgtta
    11461 catatttgca acaagcctat gccaaactgg gtacttcgtg caaaaacatc atcgcgtccg
    11521 tcccgcaaaa tctggcaacc atcgaacaat tgacctacac agacaaagat gcagaattag
    11581 acctgcaggg gttcgtggag tcctccatct ccgaagtcag ctcgatatcg ctcgaagaag
    11641 ccaattacga ctatcaggtc ttgtcccaat cggccgccgg cgaagctgtg ttggccgtcg
    11701 catcgagaaa ggatgaaatc gaacccctga ttgacgcatt caacgccgcc ggtatgaaat
    11761 tatccgcgct tgatgtggac attttcggac aatacaacgc ctacgcgcta tggataaacc
    11821 atttcgcccc cgagcttgca gccgaaaaag tcgccatttt cggcgtatat gccgcacaga
    11881 cctacgcctt ggtcatccaa gacggaaaaa tcctatacaa acaggaaacc tccgtcagcg
    11941 aagaacagct caaccaactc atccagcgca cctatcaggt aacagaagaa aaagcggaag
    12001 aaatcatcaa ctccccgcaa aaaccttccg attaccaaga aagcgtggca aactatttca
    12061 accagcagat tacccaagaa atacaaaggg tcttgcagtt ttattacacc acgcagaccg
    12121 cagacgatat gaccgacatc aagcatatcc tgctgaccgg ggaagcggcg cgccaggaag
    12181 gtatcgccca aaccgtcgcc tcacaaacca atgcagatgt acaatgcgtc catcccgcgc
    12241 gttattttgc ggacaacctc aaaacagaca aacaacaatt cgaacttgat gcgccgacac
    12301 tgaccagggc gttcggtttg gcggtacggg gattataatt atgaacaatt taatcaaaat
    12361 caacctcctc ccctacaggg aagagatgaa caagcgcaaa cagcagcagt ttaaaacgct
    12421 gatgtacggt gccgtgctga cgggcgttgc cgccgttgcg gcaacctacc tgtttatcga
    12481 caatatgatc aataaccagt cggaaagaaa cacgctgctg gaaacctcca tcgcacactt
    12541 ggataccgag ctgtcggaaa tacaaaagct caaacaggaa aaagatgcct tcctgattaa
    12601 gaaaaacaaa gtcgaggagc tccagctcaa acgcctccaa gccgcaaaaa tcctcgacag
    12661 cctgaatgag gccgtccccg gaagcaccta cctgacctcg ctggatgccg ttaccgccga
    12721 ctcttatcgg ctcagcggca ggacatccag cgacaaccgc gttgccgcca tgatgagggc
    12781 gatgcccaat accggcatat tcaagcaacc cgaattgtta agcatcaaga aaaacaattc
    12841 gcatcaagaa tttacccttc aggcaacatt acaacccatc gtaaaggcgg ccgaatccaa
    12901 agagaatccg gcttcgggaa acgcacagga ggcaaactga atggcttcta aatcatctaa
    12961 aaccaacttg gatctcaaca accttcacct gctcaacctt cctgccaggc tttttatcgc
    13021 cctgctggcc gttgccgccg tgctggggct cggttatgcc ggattgttca aaagccagat
    13081 ggaatccctt gaggaatacg aagcaaaaga aaccgaactg aaaaacacct acaaacagaa
    13141 aagtatcgac gcggccagcc tgaacaacct gagggacgaa cttgcctcaa tccgctctgc
    13201 cttcgatatc atgttgaaac agctgccgac agatgcagaa attcccaatc tggttcaaga
    13261 gcttcatcag gcaggttcga gcaacggcct gcgcttggac agcgttatgc cccaacctcc
    13321 cgtagatgac ggccccatca aaagattacc ctattccatt tccattaccg gaaattacga
    13381 acagatcagc caatttaccc gcgatgtcgg cagcctctcc cgaatcatta cccttgagtc
    13441 gctgaaaatc gcccaatctc cggaaaacgg cggcaatcct gacggcaaga gcagcatcct
    13501 gaacctcagt gccattgcca ccacctacca agcaaaatcc gtagaagagc ttgccgcaga
    13561 agcggcacaa aatgccgagc aaaaataact tacgttaggg aaaccatgaa acactatgcc
    13621 ttactcatca gctttctggc tctctccgcg tgttcccaag gttctgagga cctaaacgaa
    13681 tggatggcac aaacgcgacg cgaagccaaa gcagaaatca tacctttcca agcacctacc
    13741 ctgccggttg cgccggtata cagcccgccg cagcttacag ggccgaacgc attcgacttc
    13801 cgccgcatgg aaaccgacaa aaaaggggaa aatgcccccg acaccaagcg tattaaagaa
    13861 acgctggaaa aattcagttt ggaaaatatg cgttatgtcg gcattttgaa gtctggacag
    13921 aaagtctccg gcttcatcga ggctgaaggt tatgtctaca ctgtcggtgt cggcaactat
    13981 ttgggacaaa actacggtag aatcgaaagc attaccgacg acagcatcgt cctgaacgag
    14041 ctgatagaag acagcacggg caactgggtt tcccgtaaag cagaactgct gttgaattct
    14101 tccgacaaaa acaccgaaca agcggcagca cctgccgcag aacaaaatta agaagaggat
    14161 tactccatta tgaataccaa actgacaaaa atcatttccg gtctctttgt cgcaaccgcc
    14221 gcctttcaga cagcatcggc aggaaacatt acagacatca aagtttcctc cctgcccaac
    14281 aaacagaaaa tcgtcaaagt cagctttgac aaagagattg tcaacccgac cggcttcgta
    14341 acctcctcac cggcccgcat cgccttggac tttgaacaaa ccggcatttc catggatcaa
    14401 caggtactcg aatatgccga tcctctgttg agcaaaatca gtgccgcaca aaacagcagc
    14461 cgtgcgcgtc tggttctgaa tctgaacaaa ccgggccaat acaataccga agtacgcggg
    14521 aacaaagttt ggatattcat taacgaatcg gacgataccg tgtccgcccc cgcacgcccc
    14581 gccgtaaaag ccgcgcctgc cgcaccggca aaacaacagg ctgccgcacc gtctaccaag
    14641 tccgcagtat ccgtatccaa accctttacc ccggcaaaac aacaggctgc cgcaccgttt
    14701 accgagtccg tagtatccgt atccgcaccg ttcagcccgg caaaacaaca ggcggcggca
    14761 tcagcaaaac aacagacggc agcaccagca aaacaacagg cggcagcacc agcaaaacaa
    14821 caggcggcag caccagcaaa acaaaccaat atcgatttcc gcaaagacgg caaaaatgcc
    14881 ggcattatcg aattggctgc attgggcttt gccgggcagc ccgacatcag ccaacagcac
    14941 gaccacatca tcgttacgct gaaaaaccat accctgccga ccacgctcca acgcagtttg
    15001 gatgtggcag actttaaaac accggttcaa aaggttacgc tgaaacgcct caataacgac
    15061 acccagctga ttatcacaac agccggcaac tgggaactcg tcaacaaatc cgccgcgccc
    15121 ggatacttta ccttccaagt cctgccgaaa aaacaaaacc tcgagtcagg cggcgtgaac
    15181 aatgcgccca aaaccttcac aggccggaaa atctcccttg acttccaaga tgtcgaaatc
    15241 cgcaccatcc tgcagatttt ggcaaaagaa tccgggatga acattgttgc cagcgactcc
    15301 gtcaacggca aaatgaccct ctccctcaaa gacgtacctt gggatcaggc tttggatttg
    15361 gttatgcagg cacgcaacct cgatatgcgc caacaaggga acatcgtcaa catcgcgccc
    15421 cgcgacgagc tgcttgccaa agacaaagcc ttcttacagg cggaaaaaga cattgccgat
    15481 ctgggcgcgc tgtattcaca aaacttccaa ttgaaataca aaaatgtgga agaattccgc
    15541 agcatcctgc gtttggacaa tgccgacaca accggaaacc gcaatacgct tgtcagcggc
    15601 aggggcagcg tgctgatcga tcccgccacc aataccctga ttgttaccga tacccgcagc
    15661 gtcatcgaaa aattccgcaa actgattgac gaattggacg tacccgcgca acaagtgatg
    15721 attgaggcgc gtatcgtcga agcggcagac ggcttctcgc gcgatttggg cgttaaattc
    15781 ggcgcgacag gcaagaaaaa gctgaaaaat gatacaagcg cattcggctg gggggtaaac
    15841 tccggcttcg gcggcgacga taaatggggg gccgaaacca aaatcaacct gccgattacc
    15901 gctgccgcaa acagcatttc gctggtgcgc gcgatttcct ccggtgcctt gaatttggaa
    15961 ttgtccgcat ccgaatcgct ttcaaaaacc aaaacgcttg ccaatccgcg cgtgctgacc
    16021 caaaaccgca aagaggccaa aatcgaatcc ggttacgaaa ttcctttcac cgtaacctca
    16081 atcgcgaacg gcggcagcag cacgaacacg gaactcaaaa aagccgtctt ggggctgacc
    16141 gttacgccga acatcacgcc cgacggccaa atcattatga ccgtcaaaat caacaaggac
    16201 tcgcctgcgc aatgtgcctc cggtaatcag acgatcctgt gtatttcgac caaaaacctg
    16261 aatacgcagg ctatggttga aaacggcggc acattgattg tcggcggtat ttatgaagaa
    16321 gacaacggca atacgctgac caaagtcccc ctgttgggcg acatccccgt tatcggcaac
    16381 ctctttaaaa cacgcgggaa aaaaaccgac cgccgcgaac tgctgatttt cattaccccg
    16441 aggattatgg gtacggccgg caacagcctg cgctattgat gcgtcaaaat aagggcatat
    16501 gttttacggc atatgccctt tctttatgct ttttgccgcg accgaaatgc cgtcattccc
    16561 gcgcaggcgg gaatccagtc cgttcagttt cggtcatttc tgataaattc ctgttgcttt
    16621 tcatttctgg attcccactt ttgtgggaat gacggcggaa ggggtaaatc ctcgcaatcc
    16681 aaagcctcgt catttccaca aaaaacagca acccggaaca gcaacttaaa accccgtcat
    16741 tcccgcgcag gcaggaatcc aacttgtccg gttccggttg tttttcgtct cgtaactttt
    16801 gagccgtcat tcccgcgcag gcgggaatct aggtctgtcg gttcaggaac ttatcggata
    16861 aaacggtttc tccaaccctg cgttctagat tcccactttc gtgggaatga cgggatatgg
    16921 gtttccgtgc ggacgtgttc ggatttccgc ctgcgcggga atgacggcga cagatgccca
    16981 acggtct
//
LOCUS       DAXHPG010000028        16946 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXHPG010000028 DAXHPG010000000
VERSION     DAXHPG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16946
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.028"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(93..932)
                     /locus_tag="ACFM6F_000689"
     CDS             complement(93..932)
                     /locus_tag="ACFM6F_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC6164154.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADGAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1217..1292
                     /locus_tag="ACFM6F_000690"
     tRNA            1217..1292
                     /locus_tag="ACFM6F_000690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1250..1252,aa:Arg,seq:ccg)
     gene            complement(1357..2550)
                     /locus_tag="ACFM6F_000691"
     CDS             complement(1357..2550)
                     /locus_tag="ACFM6F_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC6164155.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVRRAETARAATPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="ACFM6F_000692"
     CDS             complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="ACFM6F_000692"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC6164156.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASMIRCDRFEAASEAVNRLMPELIAAAAQSPELKKRW
                     YAVEFLSTLSGEMLVTMIYHKRLDAEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSISAAQWNIEANRIGNIKIARLSAEEFTEAYTRKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3806..4906
                     /gene="aroC"
                     /locus_tag="ACFM6F_000693"
     CDS             3806..4906
                     /gene="aroC"
                     /locus_tag="ACFM6F_000693"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC6164157.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIRVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRSAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            4998..5423
                     /locus_tag="ACFM6F_000694"
     CDS             4998..5423
                     /locus_tag="ACFM6F_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC6164158.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETLSVEAEAAESSAAE"
     gene            complement(5497..7482)
                     /gene="parE"
                     /locus_tag="ACFM6F_000695"
     CDS             complement(5497..7482)
                     /gene="parE"
                     /locus_tag="ACFM6F_000695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC6164159.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVAGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7549..8097)
                     /locus_tag="ACFM6F_000696"
     CDS             complement(7549..8097)
                     /locus_tag="ACFM6F_000696"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC6164160.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINNRDEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQSSDNR"
     gene            8239..9534
                     /gene="serS"
                     /locus_tag="ACFM6F_000697"
     CDS             8239..9534
                     /gene="serS"
                     /locus_tag="ACFM6F_000697"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC6164161.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYKFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLEAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9612..10610)
                     /locus_tag="ACFM6F_000698"
     CDS             complement(9612..10610)
                     /locus_tag="ACFM6F_000698"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6164162.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGRYVELNELYARSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSSAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10735..11811)
                     /gene="prfA"
                     /locus_tag="ACFM6F_000699"
     CDS             complement(10735..11811)
                     /gene="prfA"
                     /locus_tag="ACFM6F_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC6164163.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKINTLDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITNALIAEHQAELLAAMGD"
     gene            complement(11901..12671)
                     /locus_tag="ACFM6F_000700"
     CDS             complement(11901..12671)
                     /locus_tag="ACFM6F_000700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6164164.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATRPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILWIAEIAAQIRGVGVADIAAITERNADSLLRP"
     gene            complement(12674..13666)
                     /locus_tag="ACFM6F_000701"
     CDS             complement(12674..13666)
                     /locus_tag="ACFM6F_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC6164165.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSEGWGADEVKHELQRLGLV"
     gene            complement(13733..14416)
                     /locus_tag="ACFM6F_000702"
     CDS             complement(13733..14416)
                     /locus_tag="ACFM6F_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6164166.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14605..15939)
                     /gene="glmM"
                     /locus_tag="ACFM6F_000703"
     CDS             complement(14605..15939)
                     /gene="glmM"
                     /locus_tag="ACFM6F_000703"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC6164167.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVIQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLVGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLAAVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16069..16920)
                     /gene="folP"
                     /locus_tag="ACFM6F_000704"
     CDS             complement(16069..16920)
                     /gene="folP"
                     /locus_tag="ACFM6F_000704"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC6164168.1"
                     /translation="MARHGWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAKTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSSEEEWARVEPVLVEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQTDTGICLMHMQGLPETMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMIGELTGEADAAARVHGSVAAAVAAVARGAQIVRVHDVKATADALKVW
                     EALGINL"
BASE COUNT         3757 a         4742 c         4547 g         3900 t
ORIGIN      
        1 tcctgatttt tgttaattca ctataaattc ccataaaaaa acggagcaga tacctgcccc
       61 gtttttattt aatccgaaat tttaatctaa atttaaaatt ttgcaccgga ttggtttgcc
      121 atataatcaa cagccgcttt gacttcgtca tcgctcaaac cggcattgcc gcctttggca
      181 ggcatcgcgt taaagccttc aagggcgtgt ttgtgcaagg tttctttgcc ttttttgata
      241 cgcggtgccc aatcgtcttt tttgcctatg ccgggaatac cgggaatcga accgccgtgg
      301 cacacctgac aggttgcttc gaagactttt ttaccgtcaa cgccgaccgc aggggctgcc
      361 gcacccttgt cttctgcctt cgcttctgcc ggagctgcac catcggcagg agcagaagct
      421 gttcctgaag cggcattgtc ggcaggcgca gcctcatcag gattcgggaa agaaccgccg
      481 cttttgttcg ccatgtaagt aatcgcccgt ttgagttcct gatcggtcag gtctgccgca
      541 ccgcctttgg caggcatggc gttaaagccg ttcagcgcgt gttggaacaa ggtatcgaag
      601 ccttgcgcga tacgcggtgc ccaatcgccg ttgtgttcca gtttcggagc gttcggcaca
      661 ttgctgtccg ccgcgtggca ttggatacag attttgccga aaatctgttc gccttggcgt
      721 tcgccgacgg ggatgccgtc gcccatcgtc aattgtccga cgggctggat acgggtctgc
      781 gttgccgctt ccgtagtggc atcgacatcg ccgaacgagc cgctgcccgc cagcttaatc
      841 aggaaataaa ggactgcaat aacaataacg ataccgctca caagggtaaa cagtgcagag
      901 ccttgggctt tgttgtcgcg gagttgtttc atttggtagg cctcgccgtc aggttaggtt
      961 gtgctgtaaa ttatagtttg gtgtgttaaa cgcagttaac aatattttgc tggattatac
     1021 tgaattcaca gggtctttcc aatcgctatc attgaaaata tgaaaaaatt tgccaacggt
     1081 atctgtataa aacaaataat cctttgaaaa taatggttta tcctcaagaa aactctcctt
     1141 atgccgccat acgccgcctg ccggcgcaag ataacctttg ccaatttgca gaatttacgt
     1201 taaccttacg ttttacgcac ccatagctca gttggaagag tgtcagtttc cgaagctgga
     1261 ggtcacaggt tcgatccctg ttgggtgcgc caattataaa gagaccgtct gaaagataaa
     1321 tatttttcag acggtctttt gacttacttc aaactcttat ttcaagactt ccgcaaatgc
     1381 gcgggcaaca tagtcggtat tcgacgtatt cagtccggcg acgcacatcc tgccggaatc
     1441 cagcaggtaa acggcaaatt cgtcgcgcag cctgcggact tgttccacgc tcaatcctgt
     1501 gtagccgaac atgccgcgct gtttgatgaa ataagtgaaa tcgcgatcgg ggatttgcgc
     1561 agttaagaca tcataaagtt tctgccgcat cgcacggatg cggtcgcgca tcatataaac
     1621 ctcgttttgc cacaaggcgt aaagttcggg gctgttcatc acgtcggcgg cgatatacgc
     1681 gccgtgcgcg ggcgggctgg agtagatgcg gcggacagtg aatttgagct gtccgaacac
     1741 caaatccgct tcttccttat tcgggcaaac cacgctcagg ccgccgacgc gctcgccgta
     1801 gagcgacagg ttttttgaga aggaattgct gacgaacaag ggcaattcca tttccaccgc
     1861 tttgcggacg gcgtaggcat cgctgtccaa atcgccgccg aagccttggt aggcgatgtc
     1921 cataaacgga atcagtttgc gcgttttgat gatgtgcaac acttcgtccc attgttgttc
     1981 cgacatatcc acgccggtcg ggttgtggca gcagggatgg aggatcagga cgctgttttc
     2041 gggcagggtg ttgaaaaacg cggtcatttc gtcgaatttc acgccaacag tggcagggtc
     2101 gtaatatggg taagtgccga cctcgaaacc tgcgccttca aaaatgccgc gatggttgtc
     2161 ccaagtcggg tcgctgacgt aggcgcgcgc ttcgggaaac cagcggtgca ggaagtccgc
     2221 cccgactttg agtgcgcccg agccgcccaa ggtttgtacg gtagcgatgc gtccttgcgc
     2281 gagcgcaggg ttgtctttgc cgaacaataa atgctgcacc gcgctgcggt aagtgtccaa
     2341 gccttccatc ggcaggtagg gcgacggcgc aggcgtggcg gcgcgggcgg tttcggcacg
     2401 gcgcacggat tccaacacgg gcattttgcc ttcgtcgtcg aaataaatgc ctatgctcaa
     2461 attgactttt tcagggcgcg ggtcgttttt gaaggtttcg accaaactca aaatcgggtc
     2521 gcccggatag tattcgatgt gtcggtacat agtccttacc ttttactttt tcaaaggatt
     2581 ttctttttca acaatacacc actttcgata tggtgcgtaa acgggaattg gtcgaacagg
     2641 gcggcgcgtt cgaccgcatg ggtttccacc aaggtgtcca aattggcacg cagcgtttcg
     2701 ggattgcagg aaatgtagat gatgttgtca aactgcgaca ccagcttcaa agtttcctca
     2761 tcgataccgg cacgcggcgg atcgacgaaa atagtggaaa atgcgtaatc cgtcaaagca
     2821 ataccgccat ccttaaggcg tttaaactca cgttttctgg tataggcttc ggtaaattct
     2881 tcagcagaca gacgggcgat tttgatgttg ccgatgcggt tggcttcgat attccattgc
     2941 gccgcgctga tggaggtttt ggagatttcg gttgccaaaa cacgttcaaa cttttcagat
     3001 aaaggaagcg taaagttgcc gtttccgcaa tacagttcga gcaaatcccc atccaatcct
     3061 tctgctgcac cgcaggccca ttcaagcatt ttctgacaca cggcggcatt cggttgggta
     3121 aaactgcctt caatttgccg ataacggaaa tcccggttgc cgaccttcaa agtttccgtt
     3181 acatagtcct gttttaagac tattttctgt cccctgctcc gcccaataac ggaaatatcc
     3241 aactgttgct gtaacgcttg cgccgcctgc atccactcag catcaagcct tttgtggtaa
     3301 atcatggtaa ccagcatttc cccgctgagc gtggacagaa attcgacggc ataccagcgt
     3361 tttttgagtt cgggggattg cgcggcggcg gcgatcagct cgggcatgag gcggttgaca
     3421 gcctcggaag ctgcttcaaa acggtcgcag cgtatcatgc ttgcgccgct ggctttctgc
     3481 cctttttcaa acatggcata aaacatttcc ccgccttcgt gccaaatacg gaactcggca
     3541 cgcatacggt aatgtttgtc cggagattcg tacacttccc actcaggaac atccaaacct
     3601 gcaaaaaggg ttttaaggta gtctttttta ccttgaagct gctgcgtgta gtcattcata
     3661 tcgctatcct gaaaaatcag accggattat aagccgattt gcctcgccgc acgcaaactc
     3721 tttgactgcc gccccttcaa tgacggacgg gcttttgtgc taaaatccgc catctttcca
     3781 cactataccg ataaagggaa aaatcatggc aggcaacact ttcggacaac tcttcaccgt
     3841 taccaccttc ggcgaaagcc acggcgcggg tttgggctgt atcatcgacg gctgcccgcc
     3901 cggcttggaa ttaagcgaag cggatatcca atttgacctc gaccgacgca aacccggcac
     3961 cagccgccac gttacccaac gccgcgaagc cgaccaagtc gaaatcctct ccggcgtatt
     4021 cgaaggcaaa accaccggca cgcccatcgc cctcttaatc cgcaataccg accagcgcag
     4081 caaagactac ggcaacatcg ccaccagctt ccgccccggc cacgccgact atacctattg
     4141 gcacaaatac ggcacgcgcg actaccgcgg cggcggcagg agctccgccc gtgaaaccgc
     4201 cgcccgcgtt gctgccggag ccgttgccaa aaaatggttg aaagaaaaat tcggcacgga
     4261 aatcaccgcc tacgttaccc aagtcggcga aaaagaaatc cggtttgaag gctgcgaaca
     4321 catttcccaa aatccttttt ttgccgccaa ccatagccaa attgccgagc tggaaaacta
     4381 tatggacggc gtgcgcaaat ccttggattc cgtcggcgcg aagctgcata tcgaagcagc
     4441 caatgtccct gtcggcttgg gcgaacctgt ttttgaccgc ctcgatgccg aaatcgccta
     4501 cgcgatgatg ggcatcaacg ccgtcaaagg cgtggaaatc ggcgcaggtt ttgacagcgt
     4561 aacgcaacgc ggcagcgaac acggcgacga actgaccccg caaggcttcc tgtccaacca
     4621 ctcaggcggc atcctcggcg gcatcagcac cgggcaagac atccgcgtca atatcgccat
     4681 caaacccacc agctccatcg ccaccccgcg ccgcagtatc gacatcaacg gcaaccccat
     4741 cgaactcgcc acgcacggca ggcacgaccc ctgcgtcgga ctgcgctccg cgccgatcgc
     4801 cgaagccatg ctcgcgttag tcctcatcga ccacgccctg cgccatcgcg cgcaaaatgc
     4861 cgacgttcag gttaatacgc ccgacattac cctttcaaac aaataaaaat ttagccaaaa
     4921 cacagacttt ataatagaat atcgagcatt gccgtgcagc cttgacgcac gggtttgttt
     4981 agaggaatac aaccgaaatg acacaagaaa ccgctttggg cgcggcacta aaatccgccg
     5041 tccaaactat gagcaaaaag aaacagaccg aaatgatcgc cgaccacatc tacggcaaat
     5101 acgatgtgtt caaacgcttc aaaccgttgg cgctcggcat cgatcaggat ttgattgccg
     5161 ctttgccgca atacgatgcc gcactgattg cacgcgtcct cgccaaccac tgccgccgtc
     5221 cgcgctatct gaaagccttg gcgcgcggcg gcaaacgttt cgatttgaac aaccgtttca
     5281 aaggcgaagt tacccccgaa gaacaggcga tcgcgcaaaa ccatcctttt gtgcagcagg
     5341 ctttgcaaca gcagtccgcc caagctgccg ccgaaacgct gtctgttgaa gccgaagcag
     5401 ccgaatcttc cgcagcagaa taaatcccca aacgaaatgc cgtctgaaaa ccgatttggt
     5461 ttcagacggc attttttcgt atgcggcaat cacggttcaa atatcgagtt ccgccgtatc
     5521 gccttcgcgt tccatccagg cacggcgggc ggcggcttcg cctttgccca tcagtttgac
     5581 gaagatgtcg cgcgtctcgt catccgcgcc ttctgggatt tgtacctgca acaggcggcg
     5641 ggtgtcgggg tgcatggtag tatctttgag ctggtctggg ttcatctcac ccaagccttt
     5701 gaaacggctg atggaataag cagtttcttt aacgccttct ttttgcagcc gctctaaaat
     5761 gccgtcaagt tcgttttggt cgagggcgta gaatttgcgg gcgggtttgc ttttaccttg
     5821 tgcgttgaca tctacgcgga acagcggcgg ctgggcgacg tagatgtgtc cgtcagcgac
     5881 cagtttcggg aagtggcggt agaacagggt cagcagcaaa acttgaatat gcgagccgtc
     5941 cacgtcggca tcggacagga tggcgatttt gccgtaacgc aggccgctta aatcgggatg
     6001 gtcgttgatg ccgtgcggat cgacgccgat ggcaactgaa atgtcgtgga tttcggcgtt
     6061 gccgaagagc tggtcggggt ggacttcaaa gctgttgagc actttgccgc gcaggggcag
     6121 gatggcctgg gtggctttgt cgcgggcgag tttggctgag ccgccggcgg aatcgccttc
     6181 gacaaggaag agttcgtttt cgcggatgtc ttcgctttcg cagtcggtca gcttgccggg
     6241 caggacggcg acgccgctgc cttttttctt ttcgattttc ttaaccgaac gcatccgcgc
     6301 ctgcgcctga cggatggcga gttcggcaat ttttttaccg aagtccacgt tttggttcag
     6361 ccacaattcc aaagggtcgc ccgctacagc ggcgacaagt ttcagcgcgt cgcggttggt
     6421 cagcttgtct ttggtctgac cttggaactg cgggtcgagg acacgggcag agaggacaaa
     6481 ggcggttttg ccgaacacgt cgtcgctttg tactttcacg ccgcgcggca agaggttgtg
     6541 cagattgatg aaattgttga cggcgttgaa cacggcttgt ttcaagcctg cttcatgcgt
     6601 gccgcccaaa ggggtgggga tgaggttgac atagctttcg ttggcacacg agccttcttc
     6661 cagccaagtc agggcaaacg ccgctccttc gccgatgctg aaatcgccgt tgtgttcgtc
     6721 tgaaatgtag ttttcgcaag agaacagcgg cacagcttct tgcgcgtcgg caatcaggtc
     6781 ggtcagatag cttttcaggc cgtcggggta gtgccaggtt tgggtgtgcg cttcgtcttc
     6841 gcctttgacc ggacgggtca gggaaacgcg cacacccggc agcagcacgg ctttggcacg
     6901 cagcaggcgt tcgagttcgg gaatgctgta attcgggctt tcaaaatatt tgccgtccgg
     6961 ccagacgcgc acttcggtgc cgctgtcttt gacggcgcat ttgcccactt gtgccaacgg
     7021 ttcgaccacg tcgccgccgg caaacacgat acggtggatt ttgccttcac gtttgaccgt
     7081 tacctcaagg cgggtggaga gggcgttggt gacggatacg cccacgccgt gcagtccgcc
     7141 tgaaaaggca tacgcgctgc cgccgtcttt tttgttgaac ttgccgcccg cgtgcagacg
     7201 ggtgaacacg agttcgacta cgggtacgcc ttcttcaggg tgcaggccga cgggaatacc
     7261 gcggccgtta tcacggacgg aaagcgaacc gtcttcatga atttgcacgt cgattgcagt
     7321 cgcgaaacca cccaacgcct cgtccgccgc attatcaatc acttcctgac agatgtgggt
     7381 cgggctgtcg gtgcgggtgt acatgccggg acgttctttg accggctcca agcctttgag
     7441 gacggtgatg ctggattcgc tgtattggtt gtttttagcc ataggaataa tctgaaagta
     7501 agaaaaacaa cgctttcaga cggcctgaaa gcgttgcgtt ccgttgtttt agcggttgtc
     7561 ggaagattgg cgggcggcaa agtcttcata actttccata ccgcgcagga agcgggaaga
     7621 gagttctttc aacgccccca aataaacgtc gcgtttgaaa tcaatcactt cgtcaacggg
     7681 cgcccaatat tgatgccaac gccagccgtc aaattcgggg tggcgggtgg cgcgcaggtt
     7741 gacatcgcaa tctcggccgg tcaggcgcag gagataccaa atctgcttct gcccgcgata
     7801 ggatccgcgc cattcgcggc gtacccagtt gttcggcacg tcataacgca gccagtcgcg
     7861 cgtgcggccg ataattttga cgtgttgcgg caaaagcccg acttcttcgt acaactcgcg
     7921 gtacatggcg gtttcggggc tttcgcccgg cttgatgccg ccttgcggaa actgccaaga
     7981 atgttcgcgc acgcgcttgc cccaaaagac ttcgtcgcgg ttgttgatta agatgatacc
     8041 gacattgggg cgatagcctt ccctgtccaa cacggtgtcg ccctccgtta aattcaatct
     8101 tgggattttc ccacaaatcg gacggttttg acaaatcgga cgatagggtt caggcatgtc
     8161 gaaacacaga cggattcggg aaaatatctt aaatttggtt tacaataata cgtttcaaat
     8221 taattcggga atcagaccat gttagatatc caattgctcc gcagcaacac cgccgctgtg
     8281 gcggaacgct tggcggcacg cggttacaag tttgatgccg cgcgtttcaa tgcgctggaa
     8341 gagcagcgaa aagccgtcca ggtgaaaacc gaagagctgc aagcctcgcg caacagcatt
     8401 tccaaacaaa tcggcgcact gaaaggtcag ggcaaacatg aagaggcgca ggcggccatg
     8461 gatcaagtcg cccaaatcaa aaccgatttg gaacaggcag ccgccgattt ggaagccgtg
     8521 caaaaggaac tggacgcatg gctgttgagc attccgaacc tgccgcatga ggacgtgcct
     8581 ttcggtaagg atgaaaccga aaacgtcgaa gtccgcaaag tcggcacgcc acgcgaattt
     8641 gactttgaaa tcaaagacca tgtcgatttg ggcgaacctt tgggtttgga tttcgaaggt
     8701 ggcgcgaaac tttccggcgc acgatttacc gtgatgcgcg gacaaatcgc ccgcctgcac
     8761 cgcgcgctgg cgcaattcat gctcgatacg cacacgctga aacacggcta caccgagcat
     8821 tacacaccct atatcgtgga cgacaccacc ttgcaaggca cgggtcagct gccgaagttt
     8881 gcagaagatt tgttccacgt tacccgtggc ggcgacgaga gcaaaaccac gcaatatctg
     8941 attccgacag ctgaagttac cctgaccaat accgttgccg gcagcattat cccgtccgaa
     9001 caactgccgc tgaagctgac cgcgcattcg ccctgtttcc gcagcgaggc gggttcgtac
     9061 ggcaaagaca cgcgcggtct gattcgccag caccagttcg acaaagtgga aatggttcaa
     9121 atcgttcatc ccgaaaaatc atacgaaacg ctggaagaaa tggtcggcca tgccgaaaac
     9181 atcctgaagg ctttggaact gccctaccgc gtgattaccc tgtgtaccgg cgacatgggc
     9241 ttcggcgcaa ccaaaacgta tgacttggaa gtttgggtac cggcgcaaaa cacctaccgc
     9301 gaaatctcaa gctgctccaa ctgcgaagat ttccaagccc gccgcatgaa ggcgcgtttc
     9361 aaagacgaaa acggcaaaaa ccgcttggta catactttga acggctccgg cttggctgtc
     9421 ggcagaacgc tggtcgccgt attggaaaac catcaaaacg ccgacggcag catcaacatc
     9481 cctgccgcac tgcaaccgta tatgggcggc gttgccaagt tggaagtgaa ataagtttgc
     9541 aagctgcctg aacgtcaaat gccgtctgaa acctggttca gacggcattt cctttaaact
     9601 tttaaaacac atcaaccgtc ggcacgaacc gcattgccgc aatcgccggt ctgtccgacc
     9661 tcgcggatat tggacagcgt aacttccgaa atattaccca acgcctcttc cgtcaaaaat
     9721 gcctgatggc cggtaaacag cacattatga caagacgaca ggcggcggaa cacgtcgtcg
     9781 gtaatcacat cgttggattt gtcttcaaaa aacaattcgc gttcgttctc gtacacgtcc
     9841 atacccaatg cgccgatttt ccggcgtttc aacgcctcaa tcgcggcaga actgtcaatc
     9901 agaccgccac ggctggtgtt gataatcatc acgccgtctt tcattttgtc gaacgccgct
     9961 tcgttcagca tatagtggtt ttccggcgtg gaggggcaat gcagcgtgat gatgtccgac
    10021 cgggcataca gctcgttcaa ctccacatac ctgccgccga ttttttccgc ttcggggttg
    10081 caaaacggat cgtaagccag caggttcatg ccgaaaccct ttaaaatccg catggttgca
    10141 ataccgattt tccccgtgcc gataacgccc gccgttttgc cgtacatatt gaaaccggtc
    10201 agaccttcca gcgaaaaatt cgcatcgcgg gtacgctgat aggctttgtg gatacggcgg
    10261 ttcaacgtca gcatcagacc gaccgtatgt tccgcaaccg attcgggcga ataggcaggc
    10321 acgcgcacga ctttcaagcc caactcttca gccgccttta aatccacatt attgaagccg
    10381 gcacaacgca acgccacagt tttcacgcca atttgcgcca atttttccaa cacgggccgg
    10441 ctgccgtcgt cgtttacaaa aatacagacc gcttccgcgc cttccgccat tttcgccgtt
    10501 ttcgcatcca gcataaaatc aaaaaactcc agctcgaagc cgaaatgccg gttggcgcgg
    10561 gtaaaatgtt cgcggtcata gcttttcgta ccgtaaatcg caatcttcat caatatgtcc
    10621 agttgtcgcc tatggttgag aaacggcata atacactgaa ttcaaacaaa tcagtagaat
    10681 atggcagatt aaaattgatt gcatacacgg catttccatt tcaaaacaca aaactcaatc
    10741 gcccattgcc gccagaagct ccgcctgatg ctcggcaatc agggcattgg tgatttctgc
    10801 caagtcgccg tccatcacaa aatccagctt gtgcagggta aggttgatgc ggtggtcggt
    10861 taccctgcct tggggatagt tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat
    10921 gagggatttg cgctcggcgg cttctttggc ttgggcttcg cgtttttgcg cgtcgttcag
    10981 gcgggcggcg aggactttca ttgcctgcgc tttgttggca tgttggctgc ggccgtcttg
    11041 gcattcgacc accatgccgg tgggcaggtg ggtgatgcgg acggcggagt cggttttgtt
    11101 gatgtgctga ccgcccgcgc cggatgcgcg gaaggtgtcg atgcgcaggt cggctgggtt
    11161 cagttcgatg tcttccagtt cgtccgcttc gggcatgacg gcaacggtgc aggcggaggt
    11221 gtggatgcgg ccttggcttt cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc
    11281 aaatttcaaa cggctgtacg ccccgagtcc gacaatacgg gcgatgactt ctttatagcc
    11341 gcccaattcg ctttcgttgg cggacacgat ttcaacctgc caacggttgc gctcggcgta
    11401 gcggctgtac atacgcagca aatcgccggc aaacagcgcg gcttcgtcgc cgcccgttcc
    11461 ggcgcgtatt tcgatgaaga tgtttttgtc gtcgtcggca tctttgggca gcagcagttt
    11521 ttgcagttcg gtatcgagcg tgttgatttt ggcttttgcc gcttcgattt cttcggcggc
    11581 aaaatctttc atttcggggt cggacaacat ttcttcggca tccgccaagt cgctttgggc
    11641 aagccgatag ttttggaaca cttcgacgac gggggtcagt tcggcgtgtt cgcgcgtgag
    11701 cttgcggtag ttgtccatat ctgccgtggc ttcgggttgc cctaaaaggt gggtaacttc
    11761 ttccaaccgg tcgccgagtt gttgcagttt ttctaagata gacggcttca taattcttcc
    11821 ataacaaacg ccgcctgaat ttcagacggc atcaaaacgc ggtcattata atatattttc
    11881 tgaatattca aaaagataat ctagggacgg agcaggctgt cggcattgcg ttccgttatc
    11941 gcggcgatgt ccgccacgcc gacaccgcgt atctgcgcgg caatttcggc aatccaaaga
    12001 atattggcag gcgtattaat ctcttttttc agcataaacg ggctgtcggt ttccaatacg
    12061 aaatccccgt cgttcaaggc tttaagcgta tcgcgcactt tacgcgcgtt cggattgagc
    12121 aacaacgaac cgatgccgat tttgaaaccc agtttcgtca acacgcgcgc ttcttccgcg
    12181 ctgcctgaga aggcgtgaac gatgccgcct tgggcaaagc ctgtctgttt gacggcggcg
    12241 gcgatgtcgg cggtggcttt gagattatgg ataatcacgc ggcggcgcag ggtttgcgcg
    12301 atggcaagct ggcggacgaa aacttggatt tgccgttcgc gctgcggcgg cgtttgggtt
    12361 ttgtcgtaaa aatccaaacc gatttcgccg acccatgcct gcggataacg tgccaacatc
    12421 gcttccaggc ggacgcaatc acgctcggca atgccgtctg aaaaccaagg atggatgccg
    12481 agcgcaatac ggatttgacc gtgttcggac ggcatttccg acagttccgc cacgctttgc
    12541 caatcctgcg ggcgcgtcgc gggaacgata aaccgccaca cacccgcctg tcgcgcttcg
    12601 gcaagcacct gcgccagatt gccgcgcaaa acagaatcgg caagatggca atgtgtgtcg
    12661 gtcagcctca tcttcacacc agccccaacc gctgcaactc gtgtttcacc tcgtccgcgc
    12721 cccatccttc agaaacggca agcgtcatca atgcggttgc ggtttccaaa ttgcatttgc
    12781 cgccgttgat gatgcctgag ttgcggaacg cgttgccttg cgcgtaaacg gcagcggttc
    12841 tgccttgtcg gacttggctg atgttgagca gcagtttgcc ttgctgcgta aagtcttgca
    12901 cggcgcggat gaaaccttcg tctgcgggcg tgttgccgtg tccgtaactt tgcaggataa
    12961 gggcgtgggc gggaagctgt ccgagtccgt cggcaagttc ttggacggca aagccgggga
    13021 taagcgtgcg gacggcgatt tttgcctgcg ggtcgggata acggattttg aggccgtctg
    13081 aaacatctgc cgcgtcctga ttcgggatgc ggacattgtg ccagccctgg gtttcgtccc
    13141 actccgccag tgcgccgaaa tgcggattgt cgaaaccggc ggcggtttcg gtgctgactt
    13201 tgctgctgcc gacggcggga taaagtttgc cgtcaaaggc aataagcgtc tgcttcaatc
    13261 cgagtgggaa tgcggcaacg gcagtcgcca gattgcgcgg cgcgtcgctg cccacggcgt
    13321 tgtacggcca ttgggagccg gtcaggacga ttggcttgtc caaaccctgc agggcgaggg
    13381 cgaacagatt ggcggtatag gcgagcgtgt ccgtgccgtg cagcaccaaa acgccgtcgt
    13441 actgcggcag cgcggcggca atgatgtcca gccaagcgca ccagttttgc agttggacgg
    13501 cggaagaatc aatcagcgtc ccacaaacgt gccaatcgaa atccatgccg tttgaaaacg
    13561 gcgcgagtgc ctgcccgacc agcgcggtat cggggcgcag gccttcgctg ctttgcgtca
    13621 tgccgatggt gccgccggta taaaggacaa aaattttctg tttcatggct catcctgaaa
    13681 atgcaaagac cgcctgaaac gcgaaatgcg tccggacggt cgaaaatctg atttactgct
    13741 gcttctgtgc cgcttttttc gctgcctttt ccgccttgcg tttggcgcgt ttttcgctca
    13801 attgtgcgcg gtaaagctga taatgtcggc gtttgcgcca ccagaaccac gccagcgccg
    13861 ccgccagcac gcccaatgcg atgaagatgc cggattgcag gctgtgcatt ttcgccatca
    13921 gccaatcgat gttgtgcgcg ccgtactcgc ccaagtaaat ccaaacgggc acggaaatca
    13981 gcgcggcaag cccgtccata atcagaaagc gcagatacga tactttgcgg ctgatgccgg
    14041 cggtaacgaa aacggcagtc cgcaaacccg gcaggaagcg ggcgacaaac agaacccagt
    14101 tgccgtattt gtcgaatttt tcctgaacct gtgcgtaacg tttcggcgtc atgatgcgcg
    14161 caatcggttt gaacttgagg attttctgcc cccagatgcg tccggcggcg aacatgatgc
    14221 cgtccccgac caatacgccg agcataccga ctgcaaacat aatatgcgga ttggtataac
    14281 ccatacccga aatcacgccg cctgttacca aggtcaaatc ctcgggaatc ggcacgccga
    14341 aaccgcagat gaccaaaacg aaaaacacgg ccgcgtagcc gtattcgaca aaaaaggctt
    14401 ccaaaagggc aaacatagcg gatattccat tgtcggagat aaaaagtcag aacaagccga
    14461 aacattttct gcgcgaaacg gcattctatc aaagattatg ccgtctgaaa gcggcagggg
    14521 gcagattcgc gccctctgcc ggacgttcag acggcattta aacaggaaaa ccgccgctgt
    14581 tgaaacggtt ttgcctgatt ttgcctaaag gctgccgatg gcggaagcaa tacgttccgc
    14641 accgtcgcgc gcccaatccg cctgccgcgc ctccaccatc acgcgcacga cgggttcggt
    14701 tcccgaagcg cgcaacacga cacgcccttt gccttcgagt tctttttcca cttccgccaa
    14761 cacgtctttt gaggcttcct gccagttttg ccctttgccg atacggacat taatcatcgt
    14821 ttgcggatac ggctgccagt cggcgcaaac ggcggcaagg tcttggttca gcgtttgcaa
    14881 tgccgccaaa acctgcaagg cggaaataat gccgtcgccg gtgttgtgtt tgtccatgca
    14941 caaaatatgg ccgctggctt cgccgccgac gagccagccg cgttggttca gctgttccaa
    15001 cacatagcgg tcgccgactt tggcgcggca gaaatccacg ccctgctctt tcagggcgat
    15061 ttccatcgcc atattggtca tgaccgtgcc gaccacgccg ccgatgttga taccttctcg
    15121 ggcgcgggct ttagcaatga cgtaaatcag gctgtcgccg tcgtaaacct taccgttttt
    15181 atcgaccatc atcaggcggt cgccgtcgcc gtctaaggcg atgccgtagt cggcttcatg
    15241 ctgtaaaacg gcggcctgga gtgtcttggt ataagtcgca ccgcattttt cgttgatgtt
    15301 gtagccgttg ggttcgtcgc cgatgctgac gacctgtgca cccagttcgt gaaacacctt
    15361 gggtgcgaca ccgtaccccg cgccgttggc ggtatcgata accaacttca aaccacgaag
    15421 gtcggaatgg ctgggaaagg tggatttgca aaattcgata tagcggtcgt ccgcaccgct
    15481 gatacggcgt gcgcgcccga ggcgtgcaga aggctgggtt ttcatttctt cgtcgatttt
    15541 ggcttcgatt tccaactcga tttcatcgga aagcttcacg ccaccctcgg caaagaattt
    15601 gatgccgttg tcggaatagg cattatgcga cgcggaaatc atcacaccgg cggacaaacg
    15661 cagcgcacgg gtcagataag ccacgccggg cgtaggcagc ggaccggttt ggataacgtt
    15721 gacacctgcg gcggtaaaac ctgccaccaa agcggcttcc agcatataac cggaaatacg
    15781 cgtatccttg ccgattaaaa cggtcggttt ctggtcagtg tcgtgctgaa ccaaaacctg
    15841 ccccgccgca tagccgagtt tcaatacaaa atcgggcgta atcggaaact gcccgacttc
    15901 gccgcgcacg ccgtccgtgc cgaaatattt ttttgccatg tgttgctccg agaatgtgaa
    15961 ccgttgtccg agattataca gtctgtttgt gccctgctgt ctgcaccgtt gatgccgtct
    16021 gaagccgccc cgtccttttc agacggcatg aagtatgcga accgctgttt acagattgat
    16081 gcccaacgct tcccacacct tcaacgcgtc cgccgtcgcc ttcacgtcat gcacccgcac
    16141 aatttgcgcg ccgcgcgcca cagccgccac agccgccgcc acgctgccgt gtacgcgcgc
    16201 cgccgcgtct gcctcgccag tcagctcgcc tatcatacgt ttgcgcgaca cgccgatgag
    16261 cagcggaaaa cccgtttccg ccatcaattc gggtaaatgc cgcatcagtg cgatattgtg
    16321 ttgtaaggtt ttgccgaaac cgaaaccggg gtcgagtgtg atgcgttgcg gggcgatgcc
    16381 tgccgcgatg cattccgccg cccgcgcttt caaataccgc gccacttcac caacaacatc
    16441 ctgatatttc ggattaatct gcatggtttc aggcaaaccc tgcatatgca tcaggcaaat
    16501 acccgtgtcc gtctggcgcg ccagcaattc gaccgcgcct tcgtcggtca acgccgccac
    16561 atcgttaata atatcgatgc cgccgagtgc caacgctttt tccataacca ccgtgcggcg
    16621 cgtgtccaaa ctgacgggaa cgccccatcc cgcgacttct accaatacag gctcaacccg
    16681 cgcccattct tcttcggacg aaacataatc cgcacccggc cgcgtcgatt cgccgccaat
    16741 gtcgagaatg tccgcaccct ctttcaaaag ccgttcggcg tgcgccaaag cggttttggc
    16801 attttgcgaa tacgcgccgc cgtcggaaaa agaatcaggc gtgagattca cgatgcccat
    16861 aattttcggc ttgtccaaac cgatttcaaa ccgtcctgcc tgccaaccgt gtcgtgccat
    16921 ctgaactcct cccaaaataa aaaaac
//
LOCUS       DAXHPG010000029        16746 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXHPG010000029 DAXHPG010000000
VERSION     DAXHPG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16746
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.029"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..371)
                     /locus_tag="ACFM6F_000705"
     CDS             complement(<1..371)
                     /locus_tag="ACFM6F_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6164169.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLAR"
     gene            complement(459..2564)
                     /gene="fusA"
                     /locus_tag="ACFM6F_000706"
     CDS             complement(459..2564)
                     /gene="fusA"
                     /locus_tag="ACFM6F_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC6164170.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            complement(2583..3053)
                     /gene="rpsG"
                     /locus_tag="ACFM6F_000707"
     CDS             complement(2583..3053)
                     /gene="rpsG"
                     /locus_tag="ACFM6F_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC6164171.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            complement(3171..3542)
                     /gene="rpsL"
                     /locus_tag="ACFM6F_000708"
     CDS             complement(3171..3542)
                     /gene="rpsL"
                     /locus_tag="ACFM6F_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC6164172.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            complement(3721..3870)
                     /locus_tag="ACFM6F_000709"
     CDS             complement(3721..3870)
                     /locus_tag="ACFM6F_000709"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6164173.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            complement(3867..4124)
                     /locus_tag="ACFM6F_000710"
     CDS             complement(3867..4124)
                     /locus_tag="ACFM6F_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164174.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRIGMPINIG"
     gene            complement(4416..8591)
                     /gene="rpoC"
                     /locus_tag="ACFM6F_000711"
     CDS             complement(4416..8591)
                     /gene="rpoC"
                     /locus_tag="ACFM6F_000711"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC6164175.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            complement(8748..12926)
                     /gene="rpoB"
                     /locus_tag="ACFM6F_000712"
     CDS             complement(8748..12926)
                     /gene="rpoB"
                     /locus_tag="ACFM6F_000712"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC6164176.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YGNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAAEKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAEDDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRQDLGDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(13116..13487)
                     /gene="rplL"
                     /locus_tag="ACFM6F_000713"
     CDS             complement(13116..13487)
                     /gene="rplL"
                     /locus_tag="ACFM6F_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC6164177.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            complement(13545..14045)
                     /gene="rplJ"
                     /locus_tag="ACFM6F_000714"
     CDS             complement(13545..14045)
                     /gene="rplJ"
                     /locus_tag="ACFM6F_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC6164178.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            complement(14275..14970)
                     /gene="rplA"
                     /locus_tag="ACFM6F_000715"
     CDS             complement(14275..14970)
                     /gene="rplA"
                     /locus_tag="ACFM6F_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC6164179.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            complement(14970..15404)
                     /gene="rplK"
                     /locus_tag="ACFM6F_000716"
     CDS             complement(14970..15404)
                     /gene="rplK"
                     /locus_tag="ACFM6F_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC6164180.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            complement(15505..16041)
                     /gene="nusG"
                     /locus_tag="ACFM6F_000717"
     CDS             complement(15505..16041)
                     /gene="nusG"
                     /locus_tag="ACFM6F_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC6164181.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            complement(16043..16321)
                     /gene="secE"
                     /locus_tag="ACFM6F_000718"
     CDS             complement(16043..16321)
                     /gene="secE"
                     /locus_tag="ACFM6F_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC6164182.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            complement(16437..16512)
                     /locus_tag="ACFM6F_000719"
     tRNA            complement(16437..16512)
                     /locus_tag="ACFM6F_000719"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16477..16479),aa:Trp,seq:cca)
     gene            complement(16519..>16746)
                     /locus_tag="ACFM6F_000720"
     CDS             complement(16519..>16746)
                     /locus_tag="ACFM6F_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6164183.1"
                     /translation="HTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVEL
                     IAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
BASE COUNT         4208 a         4321 c         3755 g         4462 t
ORIGIN      
        1 cgggccagca ggatgtgttc gcgggtttgc ggcatagggc cgtcggctgc ggaacatacc
       61 aggattgcac cgtccatttg tgcggcgccg gtaatcatgt ttttaacgta gtcggcgtgc
      121 cccgggcagt ctacgtgtgc gtagtggcgg gtttcggttt cgtattccac gtgcgaggtg
      181 ttaatggtaa taccgcgtgc tttttcttcg ggtgcgttgt cgatttggtc gtaagctttt
      241 gcagcaccac cgaatttttt agccaaaata gtagtcaaag cggcagtcag ggtggtttta
      301 ccatggtcaa cgtgaccgat ggtgccaacg tttacgtgcg gtttgctacg ttcgaatttt
      361 tccttagcca tgagctaatt cctttacatt aaagatcgat taaagaacaa tgccgtctga
      421 aaaatcattt cagcctttcg gacggcctat ttgcctgatt agcctttacg ggcttcagtt
      481 acagcagcag ctatgtgagc aggagcttca gaatatttct tgaactccat agagtaagta
      541 gcgcggcctt gggttgcaga acgcaggtcg gtcgaataac cgaacatttc tgccagaggt
      601 acttcggcac ggactttttt accgccgata ccgtcatcat ccatacccaa tacaacaccg
      661 cgacggcggt tcaagtcgcc cattacgtcg cccatgtatt cttccggagt ttccacttca
      721 actgccataa tcggctcaag cagggcagga gatgcttgac gcataccttc tttaaacgct
      781 tgagaagcgg ccaattcaaa tgccaattgg gaagagtcga catcatggta agaaccgaat
      841 accagacgga tacgtacatc aactacagga tagccggcaa cgataccgtt aggcaatgta
      901 tcgcggatac ctttatcgac agacggaatg aattcgcgag gaatcacgcc acctttaatt
      961 tcatcgataa actcgtaacc ttcgcccccc ggttccatag gttccatttc aatcacaacg
     1021 tgaccatatt gacctttacc accggattgt ttcgcgtgtt tgtattcagc tttaacggct
     1081 ttgcggatag tttcacggta agccacttga ggtgcaccga tatttgcttc cacaccgaat
     1141 tcgcgtttca tacggtcaac aataatttcc aagtgcagct cacccatacc ggaaataatg
     1201 gtttgaccgg attcttcgtc tgtacggaca cggaaagaag ggtcttcttt agccaagcgg
     1261 ttcagggcga tacccatttt ctcttggtcg gctttggttt tcggctcaac ggcaatatgg
     1321 attaccggct cggggaattc catacgttcc aagataatcg gcgcgctttc cgcacacaag
     1381 gtttcaccgg tagtaacgtc tttcagacca atagcggctg cgatgtcgcc ggcgcgtact
     1441 tcttcgattt cagtacggtc tgcggcagtc atttgtacca aacgaccgat acgttcgcga
     1501 gtgcctttta cggagttcaa tacggtatcg ccggatttta ctacgcctga gtaaacgcgg
     1561 ataaaggtca gctgaccgac gtatttgtcg ttcaacattt tgaacgccaa tgcagagaat
     1621 ttctcttcat cgctggcttg acggctgtcg gcttcctcgg tattcgggtt gacaccttga
     1681 accggaggaa tatcggtagg agctggcagc aattctacaa ctgcgtccaa catacgttga
     1741 acacctttgt ttttaaatgc agaaccacac agcataggct gaatttcgcc tgccaaagta
     1801 cgttgacgca acgcgcctac gatttcttct tcggtcagct cgtcgccgcc taagtatttg
     1861 tccatcagtt cttcgctggc ttcggctgcg gcttcaatca tattttgacg ccattcttcg
     1921 gcagtttcga ccaattcggc aggaatatcg ccataggtaa aggttgtacc tttatcggct
     1981 tcattccaaa tgatggattt cattttcaac aaatcaacca caccgctgaa gttgtcttcc
     2041 gcaccaaccg gaatgacgat aggtacaggg tttgcgcgca aacgggtttt catttgctcg
     2101 acaacgcgga agaagttggc accctgacgg tccattttat tgacaaacgc caagcgcggc
     2161 acttggtatt tgttggcttg ccgccatacg gtttcagatt ggggttgaac accgcccacc
     2221 gcgcagtaaa ccattaccgc gccgtccaat acacgcatag aacgctctac ctctacggta
     2281 aagtcaacgt gtcccggggt gtcgatgatg ttgaagcggt gctcggggaa ttgtttcgcc
     2341 ataccggacc agtaggaagt aacggcagcg gaggtaatgg taataccgcg ctcttgctct
     2401 tgttccatgt agtcggtagt agccgcaccg tcatgcactt cgcccagctt gtgggtcaaa
     2461 ccggtataga acaaaatacg ttctgtcgtc gtggttttac ccgcatcgat atgggcggaa
     2521 ataccgatgt tacggtacag gctgatcggg gtcttacgag ccattttatt agcctttcaa
     2581 aattagaaac ggaagtgaga gaatgctttg ttggcttcag ccatacggtg tacttcttca
     2641 cgttttttca acgcaccgcc acggccttcg gacgcatcaa tcaattcgcc tgccaaacgc
     2701 aggtccatgg atttctcacc acgtttgcgg gccgcatcgc gaacccagcg cattgccaaa
     2761 gccaaacggc gtgaaggacg aacttcaaca ggaacttggt agtttgcacc acctacacgg
     2821 cggcttttca cttccacgat aggtttggcg tttgcaatgg cttcgttaaa tacttcgatt
     2881 gctactttgc cggttttttt ctcaatctgt tccaacgcac cgtaaacgat acgctcggca
     2941 acggattttt taccgtcaat catcaatacg ttcatgaatt tggtcaactc gacgctgccg
     3001 aatttaggat ctggcagtac gtcgcgcttg gggacttctc tacgtcttgg cattttaatt
     3061 tcctttaatc tattcagtcg ggtctattcc catgaatacc caattgagta ttcacttact
     3121 cggccgttgt tcagcttagg cggccgacgt gcctatttaa gtcccagtaa ttatttagga
     3181 cgcttagcac cgtatttgga acgggcttgt ttacggtctt taacgcctgc agtatccaaa
     3241 gaaccgcgta cagtgtggta acgcacacct ggcaagtctt ttacacgacc gccgcgaatc
     3301 aatacgacac tgtgctcttg caggttgtga ccttcgccgc cgatgtatga aatgacttca
     3361 aaaccgttgg tcaggcggac tttacatact ttacgcaatg cagagttagg ttttttaggg
     3421 gtagtcgtgt atacacgggt gcacacgccg cgtttttgcg ggcaggcttc cagtgcgggc
     3481 actttgttta cgtacacggg cttttgacgg cctttgcgta ccaattggtt gatagttggc
     3541 atattttctc gtcctgttga gttaaatact tgccgacacc atgtcggcaa gagtggaatt
     3601 atatttttat tgcaagtata cagtcaagca taacgacaag aagaaagatg attttcttgt
     3661 ttgccgataa atatttaccg acatccctca caatcagtcg gacgactgaa gaaaatactg
     3721 tcaatacgtt ttttctatac tgacaaaccg ttccttgccg ctgcgcgaat aaaaggcggg
     3781 catattgctg ttgatcttgt tgtaacggaa atttatttgg ggtgtaaatc ctttccacga
     3841 cagtttttta tgccataaag ccgcgctcat ccgatgttaa tcggcattcc tatccggtca
     3901 ccccgaacat cgccgcctgc cgcgctttta ggaacttcag acggcattgc atcgcgaacc
     3961 ggcggtacat cctcggcaaa cggcaggagg gcgaggcttt ccccttcaga cggcatcgca
     4021 atccgattac cgacgttaat cagttcgaca ggtacgccga tatcctcggc aaacgtcgcc
     4081 gaagtcagca ggcagcctgc aaaaaagaga tttctgggtt tcatatttat tttttaaagg
     4141 aaatgccgtc tgaagtttca gacgacattt gtttaaatgg aaaacattaa gacgctactt
     4201 tttttgaccg ccgaaaccaa catccctagc aaagttattt tggtctccgg acgtaccgac
     4261 gacacccgcc acctcgttgg tctccggccc gaaaatacct aaggaataat ccacacgcct
     4321 caaaatgacg aaaacgcttg tttttgcctt aatcagacaa agaaaaaccg caagcttaaa
     4381 gcttgcggtt ttgtattgaa taaacgcacc aaagattatt catccgttac ttgggtttca
     4441 gcagtctcct gttccacctc ttgccattgt tgatgacggc tgcggtggta agtcaaaccg
     4501 gtaccggcag gaatcaggcg gccgacgatg acgttctctt ttaaaccgcg cagttcgtct
     4561 tgtttgccca taatcgcggc ttcggtcaga acacgggtcg tttcttggaa cgatgcggca
     4621 gaaatgaaac tgtcggtgga cagggaggct ttggtaatac ccagcaatac gttttcgtaa
     4681 cgtgccggct ctttgccttc ttccaaagct ttttcattgg ccgccatcac atcgccgcgt
     4741 tcgacctgct ctccggtaat gaacccggtt tcgccggcat ccgcaatgtt cacacggcgc
     4801 aacatttgac ggatgatgac ttcgatgtgt ttatcagaaa tcttcacacc ttgcagacgg
     4861 taaacctctt gcacctcttg gacaatgtag cgtgccagtg cttcgatacc ttgcaaacgc
     4921 agaatatcat gcggatcaac agctccgtcc acgatggttt caccgcggtt taccacttga
     4981 ccgtcgtgta ccagaatttg tttctctttg gaaatcaagg tctcgtatgc tacaccgtcc
     5041 acgtcagtaa caatcagacg ttgcttgcct ttggtctctt tgccgaagga aacggtaccg
     5101 gtaatttccg ccaacatacc ggcatctttc ggcacgcgtg cttcaaacaa ttcggcaacg
     5161 cgcggcagac caccggtaat gtcgcgggtt ttggaagagg cttgcgggat acgcgccaat
     5221 acgtcgcctt taccgatttc ctgaccttcg cgtacggtaa tcaccgcacc aacggggaat
     5281 gccatcgaaa ccggagtgga cgtaccggga atacagattt ccacgccgtt ttcatccaac
     5341 aattttacag tcggacgcag cagtttggac gcgctgctgg aacggcgttt accgtcaatc
     5401 accaccaaag tggacaaacc ggttacatcg tcagtttgtt tcgcaacggt aacaccctct
     5461 tccacgttct caaatttcac catacctgca tgttcggtga tcatcggacg ggtatgcgga
     5521 tcccaggttg ccaaggtttg accggcttta atggccatac cgtcttgtac cagcaggatg
     5581 gcaccgtaag gtactttgtg gcgttcgcgt tcacggccga tatcgtcgtg aatcacgact
     5641 tcacaagaac ggccgatgac aaccaactcg cctttattgt tggcaacata acgcatttgg
     5701 ctgctgaatc gtgccgtacc gttggatttg gcttccactt ggctggctgc tgccgcacgg
     5761 gatgccgcac caccgatgtg gaacgtacgc atagtcagct gggtacccgg ttcgccgatt
     5821 gattgggcgg caatcacacc aactgcctca ccggcattga ccagcttacc acgtgccaag
     5881 tcgcgaccat aacagtgcgc gcacaaaccg tggcgggttt tacaagtaat cggcgtacgg
     5941 actttgactt catcgacacc ggattggtca atcatatcca ccagtttttc agtcaacaac
     6001 gtaccggctt caaccaaggt ctcgccgctt gacggatcga caacgtcaga cgcagtaaca
     6061 cggcccaaaa tacgatcgcg caatgcttca atcacatcac cgccttgtac cactgccttc
     6121 ataacaaagc cgtctgaagt accgcaatcg tcttcaacaa cgaccaaatc ttgagttacg
     6181 tctaccagac gacgggtcag gtaaccggag ttcgcggttt tcaatgcggt atccgccaaa
     6241 cccttacgcg caccgtgggt cgcaataaag tattgcaata cggtcagacc ttcgcggaag
     6301 tttgaggtaa tcggcgtttc aataatcgag ccgtcaggtt tcgccatcaa accgcgcata
     6361 cctgacaact gtttaatctg agccgcagaa ccacgggcac cggagtctgc catcatatag
     6421 atggagttga atgactcttg atcgacttcg ttgccggcac ggtcgataac tttttgtttg
     6481 gacaggttgt ccatcatcgc cttagcgatt ttatcgccgg cacgacccca aatatcgacc
     6541 accttgttgt agcgttcgcc gttggtaacc aaaccttgac ggtattggtc ttcgatttct
     6601 ttaacctcgg cattggcttc agccagcaag gccgcttttt cttttggaat ttccatatcg
     6661 tcaacggcaa tggaaatacc gccttttgcc gcaaatccga aaccggtgta catcaggtgg
     6721 tcagcaaaga taaccgtatc gcgcaagccg cacaggcgga acgatgcgtt aatcagttta
     6781 gaaatttctt ttttcttcaa cgctttgttg acatattcaa acggcaggcc tttcggcagg
     6841 atttcgctca acaatgcacg gccgacggtt gtttcgtaac ggttaacgac aggctcaaac
     6901 tcacctgctt cgtttttcac ccattcgcgc agacgtacgg tgatttttgt acccagctcg
     6961 acctgtttgg tatggtatgc gcgatgcact tctttcacat cggcaaacag gctgccttcg
     7021 cctttggcat tgatacggtc gcgggtcatg tagtacagac ccaatacgat atcttgggaa
     7081 ggtacaataa tcggttcgcc gttggcagga gacaatacgt tgtttgaagc cagcatcagc
     7141 gtgcgtgctt ccatttgtgc ttccaagctc aatggaacgt gtaccgccat ttggtcgccg
     7201 tcaaagtcgg cgttgaacgc ggcacacacc aatgggtgca actgaatcgc tttaccttca
     7261 atcaagatag gttcaaacgc ttgaataccc aaacggtgca gggtcggcgc acggttcagc
     7321 ataatcggat gttcgcggat gacttcttcc aagatgtccc atacttccgg tacttcttgc
     7381 tctaccaatt ttttcgctgc tttaacggtg gaagccagac cttgtttttc caatttgtgg
     7441 aaaatgaacg gtttgaacag ttccaaagcc atttttttcg gcagaccaca ttggtgcaga
     7501 cgcaggtatg ggcctacggt aatcacggaa cggccggagt agtccacacg tttgcccaac
     7561 aggttttgac ggaagcgacc gcctttacct ttaatcatgt ctgccaatga tttcagcgga
     7621 cgtttgttgg caccggtcat ggctttaccg cgacgaccgt tatccaacag cgagtcaact
     7681 gcttcttgca acatacgttt ttcgttgcgg acgataatgt cgggcgcgtg cagttccagc
     7741 aggcgtttca gacggttgtt gcggttgatg acgcggcggt acaaatcgtt caaatcggaa
     7801 gtggcaaaac ggccgccgtc caacggaacc aaaggacgca aatcgggagg taataccggc
     7861 agcacatcca taatcatcca ttccaatttc ataccggaac gatggaaggc ttccaatact
     7921 ttcaagcgtt tggcgatttt tttgattttg gtgtcggaac cggtcgattc cagctcttga
     7981 cgcaggattt cgatttcgcc tgctacattc aaggtacgca gcaattcgcg gataccttct
     8041 gcacccattt tggcatcgaa gtcgtcgccg tattcgtcca gcttgttgta gtaatcgtct
     8101 tcagtcagca attggcggcg ttgcagcgga gtcataccgg gatcggttac cacaaatgct
     8161 tcaaagtaca atacgcgctc gatgtcgcgc aaagtcatgt ctaacaccat acccaagcgg
     8221 gaaggcaggg attttaagaa ccaaatgtgt gcgacgggcg cggccaattc aatgtgaccc
     8281 atacgttcgc ggcgtacttt ggacagggtt acttccacgc cgcatttttc acaagttaca
     8341 cctttaaatt tcaagcgttt gtatttaccg cacaagcatt cgtagtcttt gaccgggcca
     8401 aagattttgg cgcagaacaa gccgtcgcgc tcgggtttga acgtacggta gttgatggtt
     8461 tcaggttttt taacttcgcc ataagaccat gagcggatgg tttcgggaga ggcaataccg
     8521 attttaatgg catcaaactc ttcttccatg ccggcagttt gcaacggatt aaataagttc
     8581 aacaaattca tttttgctcc ttgaaggaag tatttttacc gcgtggcgga ttttcgatat
     8641 ggggaaacgg tacttttgat ttcagacggc ctaaatgccg attgcttcat tcttattctg
     8701 aaaccacttt ttcagacgac cctgaaaggc cgtctgaaaa cttttgttta gtaacgttcc
     8761 aaatcgatat ccaagcccag tgagcgaatc tctttgacca atacgttgaa ggactcgggc
     8821 ataccggcat cgattttgtg ttcgcctttg acgatgtttt cgtacatttt ggtacggccg
     8881 ttcacgtcgt cagacttcac agtcagcatc tcttgcagcg tgtatgccgc gccgtatgct
     8941 tccaatgccc aaacctccat ctcgccgaaa cgttggccgc cgaactgggc tttaccgccc
     9001 aaaggctgct gggtaaccag actgtacgga ccggtagaac gggcgtgcat tttttcgtca
     9061 accaagtggt gcagtttcag atagtgcatc acacctactg taaccttgcg atcaaatgct
     9121 tcgcctgaac ggccgtcata cagcgtgatt tgggttttac tgtcgttgaa gcccagtttt
     9181 tcaacctcag gatcgtcgct cggataagcc aagttcagca tttcgcggat ttcagactct
     9241 ttcgcaccgt cgaatacagg agaggcgaaa gatgcacctt tgcgcaggtt ggaggccagt
     9301 tcgatgattt cttcatcagt cagggcatcc aaatcttctt tcttaccgct gccgttgtag
     9361 agtctgttca agaactcgcg caactcgcct gctttgcgtt gctctttcag catacggtcg
     9421 atacgctcgc cgataccttt tgctgcccaa cccaagtgaa cttccaaaat ctgaccgatg
     9481 ttcatacggg aaggtacgcc caatgggttc agtacgatgt ctaccggacg gccgtccgcc
     9541 atgtaaggca tgtcttccac tggcagaatg cgcgatacca cgcctttgtt accgtggcgg
     9601 cccgccattt tgtcgccggc ttgcagacgg cgtttgatgg cgataaaaac tttcaccatt
     9661 ttttgtacgc cgggttgcaa ttcgtcgcct tgggtcagtt ttttcttctt gatttcgtat
     9721 aactcgtccg cttcttcgcg tttttgttgc aggctcactt taatcagttc caactgcttg
     9781 gccaaatctt catcggtcag acggatatcg aaccaatcgt gcctgctcgg cagacccgcc
     9841 agatattcgg tcgtgatttc actgcctttg gccagcttca tcgggccgcc gttggctttc
     9901 tgaccgacaa tcatacgctc gatacggtcg aatgcgtcat tatcgaaaat acgcaattga
     9961 tcgcctaaat cttgacggta acgtttcaat tcagaatcaa taatggattg agcacgtttg
    10021 tcgcgttgaa taccttcacg agtgaagact tgaacgtcga taacggtacc gctcatgccg
    10081 gtaggcatac gcaatgaagt atcttttacg tcagatgctt tttcaccgaa gatggctcgc
    10141 agcagttttt cttccggagt cagttgggtt tcgcctttag gcgttacttt accaaccaat
    10201 acatcgccgg cttctacttc cgcaccgata taaacgatac cggattcgtc caaacggttt
    10261 tgcatacgct cggacaagtt cggaatatcg cgggtaatgt cttccgcacc cagcttggta
    10321 tcgcgggcaa caacattcaa ttcctcaatg tgaatcgaag tatagcggtc gtcctcagcc
    10381 actttttcgg aaatcagaat cgagtcttcg tagttgtaac cgttccacgg catgaaggcg
    10441 atggtcatgt tttgacccaa agccaattcg cccaagtcgg tggacgcgcc gtcggccacc
    10501 aaatcaccgc gttgcaaaac atcgccggct ttgacggcag gacgctgatt gatattggta
    10561 gactggttgg aacgggtgaa tttaaccaag ttgtaaatat cgacacccac ttcaccggca
    10621 gtcgcttcgt cgtcatggac acggatcaca acgcggttgg catcgacata ctcgaccacg
    10681 ccgcctcggc gggcaacgat tgcagtagca gagtcaacgg caacggaacg ctcgataccg
    10741 gtaccgacca tcggtttttc aggacgcaag caaggcactg cctgacgttg catgttggca
    10801 cccatcaatg cgcggttcgc gtcatcatgt tccaagaatg gaatcaggga tgctgcaacg
    10861 gataccactt gaccagttgc cacgtccata tattggacgc ggtcgggcgt tgccataatg
    10921 gtttcgcctt tttcacgaca ggtaaccaaa tcgccaatca gattgccatc tgaatccaaa
    10981 tcggcattcg cctgtgcaat cacatagcgg ccttcttcga tggcagacaa gtaatcgatt
    11041 tcctcggtta ctttgccgtc gataacgcgg cggtaaggcg tttccaagaa accgtaatca
    11101 ttggtgcgcg cataaacgga caatgagttg atcaaaccga tgttcggacc ttcaggcgtt
    11161 tcaatcggac atacgcgacc gtagtgggtc ggatgcacgt cccgcacctc aaatcctgca
    11221 cgttcgcggg tcaaaccgcc cggacccaat gcagatacac ggcgtttatg ggttacttca
    11281 gacaaggggt tggtctgatc cataaactga ctcaattggc tggagccgaa gaattcttta
    11341 atagcggcag aaacaggttt tgcattaatc aaatcgtgcg gcatcaagtt ttctgattcc
    11401 gcctgattca aacgttcttt tacagcacgt tccacacggg ccaaaccgct gcggaattgg
    11461 ttttcagtca gctcgcctac cgaacgtact cggcggttgc ccaagtgatc gatatcgtca
    11521 acttcaccat ggccgttacg caactcgacc aaagtcgcaa tcgaggcgac aatatcttcg
    11581 acgctcagga cataaccgcc tttttcggca gcaccggcaa acgtttcgtt cagcaggcgg
    11641 ccgtaccaag agttttgttg ggcttcggac agtttttgtt cgtatgtgcg cgtattaaat
    11701 ttcatacggc ctacgcggga caaatcgtag ctgtcttcac tgaagaacaa gcggttaaac
    11761 aattgctcga ccgcctcttc ggtgggcggt tcgcccggac gcatcatacg gtaaatcgca
    11821 acacgcgccg cctgccggcc ggcagtctca tccgtacgca aggtgttgga gatataagca
    11881 ccctgatcca actcattgat gtaaagggtc gtaatttctt ttacgccgtt gatatcaaat
    11941 ttggccaaca actcttctgt aatttcatca ttggcagaag ccaatacctc gccggtttcc
    12001 gaatcaatca gatcggcagc caatgcttta cccagcaggc tttccggttc tacatccaaa
    12061 cgggtcaggc ctgcattggt aatatcacgg atatttttcg cagtaatgcg cttacctttg
    12121 gcaaccaata cattgccttc tttatccaag atatcgacct tggcagtttc gcctttcaga
    12181 cggcctgcga ccaaatcggt ttgaacaccg tttgaagaca aatagaacgt ttctttgtcg
    12241 tagaaaatat ccaagatttg ctcattgccg tagcccaaag ccttcaacaa aatcgttacc
    12301 ggcattttac ggcggcggtc gatacggaaa tacagcaaat ctttcgggtc aaattcaaaa
    12361 tccaaccatg aaccgcggta gggaatgata cgggcggaga ataacaactt gccggaagag
    12421 tgcgtcttac ctttgtcatg ctcgaagaat acgccgggcg aacggtgcaa ctgggagaca
    12481 atcacacgct ctgtgccgtt aatcacaaaa gaaccgctcg gggtcatcaa cggaatttcg
    12541 cccatataca cttcgttttc acgaacttct tttaccgtcg gtttagatgc ttccttatcc
    12601 aaaatcacca aacggatacg cgcgcgcaag ggggctgcat aagtgattcc gcgcaactga
    12661 cattcgggaa tatcgaacaa aggctcgccc aatgtgtaat gcacaaactc caatcgcgca
    12721 taaccgttat ggctcacaat cgggaaaata gaattaaatg ccgcctgcag accgtcatcg
    12781 gtacgtttgt caaaagcatt ttccagctgc aaaaacttcg cataagaatc aatttgggtt
    12841 gctagcaaga aaggaacttc caaaacattt tcccgctttg caaaactctt acggatacgt
    12901 tttttctcgg taaacgaata gttcatacac actccgaaaa gtaattttca ataataggcc
    12961 gtctgaaaca acggtacgca tcaatctgat atttacattt atttgcaatg acttgataaa
    13021 aactatgcaa ataaatgtaa acaatacttg atgtttattt taagaagcaa aataaggctg
    13081 gcagaaaact gccagccttc ataagaagca tcaaattatt tgatttcgac tttagcgcct
    13141 gcttcttcca gttgtttttg gatgtcttcg gcttcagctt tagaaacacc ctctttaatg
    13201 gttttaggtg cgccgtcaac gatgtcttta gcttctttca gacccaaacc ggtaattgca
    13261 cggacaactt taatcacgcc gactttttga tcaccggcag aagccaaaac gacatcaaat
    13321 tcggtttttt cttcagcatc ggcagcaccg gcaccagcag gacctgcaac tgcaacagca
    13381 gcagcagaaa caccgaattt ttcttcaaaa gctttaacca agtcgttcaa ttccattacg
    13441 gtcaaagaac caactgcttc caaaatgtct tctttagtaa tagccatgct attttactcc
    13501 aaatattgta ttaaaaaata attgattaac agaaacaaaa tcgattaagc ggcttcttcg
    13561 ccggcttttt tctctgccaa agcagccaaa ccgcgcgcaa agcccgatac aggagcttgc
    13621 ataacgaaca acagtttgga caacagctct tcgcggctcg gaatagaagc caactcagca
    13681 acctgagcag cattcattac ttcgccattg taagaaccgg ctttaacgac aattttgtca
    13741 tcttttttcg cgaattggtg caacacttta gcagcagcaa cagcatcttc agaagcagcg
    13801 taaaccaacg gaccaaccat ttgatcggcc aattctgcaa atgaagtacc ttgcactgca
    13861 cgacgagcca aagtattttt cagaacacgc aaataaacgc cttctttacg tgcattcgca
    13921 cgaagctcag tcatactgga aacactgata ccgcgatatt cagcgactac gagggtttga
    13981 gcgttagcaa ttgccgcgct aatttcctcg accgccactt tcttggtttc aatattgaga
    14041 ctcaaggtct acctcccact gtttataaac aggataccaa aacgatatcc caccagcgcg
    14101 gtaacctaaa aagacatctt acaagcaatc ttgcaatgtg tttcaggact accgtctgcg
    14161 tagggcagtt acgattaaat cttacgatcc ctacggtctt ggacatctac ttaattttaa
    14221 gtagcccaaa ttcaagcagc ccagaaaacc aaactgcttg aaaattcctt aagattagtt
    14281 atttacgctt gatgtatcaa cgcgaatacc caaacccatg gtgctagaca cagcaacttt
    14341 tttcagatac tgacctttag cggcagcagg cttggcttta acgatagcat ccagcaacgc
    14401 atcaaagttc tctttcaaat cagcttcagc gaatgaagca cgaccaatcg ttgcatgaac
    14461 aatacctgcc ttgtcggtac ggtactgcac ttgacctgct tttgcatttt ttactgcttc
    14521 ggcaacatta ggggtaaccg tacctacttt agggtttggc atcagaccac gaggacccaa
    14581 gatggtaccc aattgaccaa caatacgcat tgcatcggga gaagcaataa caacgtcaaa
    14641 gttcagattg cctgctttga tttcagcagc caaatcttcg aaaccgacga tatctgcacc
    14701 agcttcttta gcagcttctg catttgcacc ttgagtaaat acagccacac gggttatctt
    14761 accggtgcct ttaggcagaa cgaccgaacc acggataact tggtcagatt tacgcggatc
    14821 aacgcccaag ttgaaagata cgtcaacaga ctcgtcaaat ttagcagtcg ctgctttttt
    14881 taccaaagca attgcttcat caattgcata taatttattg gcttccacag aagagcgaag
    14941 agctttcaag cgtttagata ctttagccat tatacaacac cctccacatc caagcccatt
    15001 gagcgagcag aacctgctat agtacggaca gccgcatcca agtcagcagc agtcaaatca
    15061 ggatctttag ttttagcaat ttcttccaac tgggcacggg tcaatttacc cactttgttg
    15121 gtcagaggat tagaactacc tttttgcaaa ccggcagcct ttttcaacaa gatagaagct
    15181 ggcggggttt tcatcacaaa tgtgaatgat ttatctgcaa atgcagtaat cacaaccgga
    15241 atcggtaagc caggctccat accttgggtt gcagcattaa atgccttaca aaattccata
    15301 atattcaaac cgcgctgacc caaagcagga ccaaccggag gagatggatt ggctttacct
    15361 gcaggaattt gcagtttaat atagccgata attttctttg ccacttaacg aactcctaaa
    15421 aacgggtaag acgcggaacc atttccgctc cccaagttaa tttttgattc tatcctctta
    15481 ttgctttttt ttcaagtata aaaatcagtt aatcttttca acctggctga actccagctc
    15541 aacgggtgtt tctctaccaa atatctgaac agacacgcgt aacttattcc gttcataatt
    15601 gacctcctca accaccccgt taaaatccgc aaacggccct tcatttacac gaacctgttg
    15661 accgacctca aattcaactt ttggtttcgg cttctctatg ccggtctgaa cctgctgtaa
    15721 aataatttca gcctctctct gactaatcgg cgtaggtcta ttagccctcc ctccaataaa
    15781 accggaaaca cggggggtgc tttttacaag atgccaagag tcatctgtca tttccatctc
    15841 aactagcaca taaccaggat atgactttct ttcactaata gtcttacgac cattgcggat
    15901 atcaacaact ttctctacag gcaccagaat ttgtccgaaa taatctccca tctcctcacg
    15961 ggcaatgcgc tcttccaata ttcgttggac attcttctca aaccccgaat acgcctgtac
    16021 aacataccat tttttcgaca tctcaacctt cccttctcag caatacatca aaaaataacc
    16081 acgaaattgc tgtatctgcc gcatagataa atatagaaag cacagcaaca aacactataa
    16141 caaatacagt cattctgaca gcatcttcac gcttaggcca aaccaccttt ttgaattcgg
    16201 accaagaatt tgagaaatat gcaaaaaacc cttccttacc ggaattagat gcagattctt
    16261 tatcttgaac aaccagttga tccactttaa cgttcttttt ttcaggcgta tgttctgtca
    16321 tattatttat ccatcatagc atctgtctat tctcaatcca tgtaaatggc aagagagttt
    16381 actaaataac aaatacaaaa aaaattaacc ggcacaaggc cggttaattt tttatttggc
    16441 aggccaagag ggtctcgaac ccccaaccct cggttttgga gaccgatact ctaccaattg
    16501 agctattggc ctctaaactt aagcgataac agaagaaacc acgccggcac ccacggtacg
    16561 gccgccttcg cgaatcgcaa agcgcaaacc ttcttccata gcgataggcg caatcagttc
    16621 tacggtgatg gttacgtttt cacccggcat taccatttcc acaccttctt ccaaagtaac
    16681 cgcgccggtt acgtcggtgg tacggaagta gaattgcgga cggtagttgg cgaagaacgg
    16741 ggtatg
//
LOCUS       DAXHPG010000030        16478 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXHPG010000030 DAXHPG010000000
VERSION     DAXHPG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16478
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.030"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            209..1273
                     /locus_tag="ACFM6F_000721"
     CDS             209..1273
                     /locus_tag="ACFM6F_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC6164184.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1414..1737
                     /locus_tag="ACFM6F_000722"
     CDS             1414..1737
                     /locus_tag="ACFM6F_000722"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC6164185.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1809..2132
                     /locus_tag="ACFM6F_000723"
     CDS             1809..2132
                     /locus_tag="ACFM6F_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC6164186.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2179..2382
                     /gene="tatA"
                     /locus_tag="ACFM6F_000724"
     CDS             2179..2382
                     /gene="tatA"
                     /locus_tag="ACFM6F_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC6164187.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2386..3072
                     /gene="tatB"
                     /locus_tag="ACFM6F_000725"
     CDS             2386..3072
                     /gene="tatB"
                     /locus_tag="ACFM6F_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC6164188.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLSDGISDVMPSERSYTSAEILGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3086..3856
                     /gene="tatC"
                     /locus_tag="ACFM6F_000726"
     CDS             3086..3856
                     /gene="tatC"
                     /locus_tag="ACFM6F_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC6164189.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKISVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3909..4517
                     /locus_tag="ACFM6F_000727"
     CDS             3909..4517
                     /locus_tag="ACFM6F_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC6164190.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPADINETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRSPLSALKP"
     gene            4526..4879
                     /locus_tag="ACFM6F_000728"
     CDS             4526..4879
                     /locus_tag="ACFM6F_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC6164191.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4869..6683
                     /locus_tag="ACFM6F_000729"
     CDS             4869..6683
                     /locus_tag="ACFM6F_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC6164192.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIIPFTFALRQQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6873..7550
                     /gene="misR"
                     /locus_tag="ACFM6F_000730"
     CDS             6873..7550
                     /gene="misR"
                     /locus_tag="ACFM6F_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC6164193.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7563..8969
                     /gene="misS"
                     /locus_tag="ACFM6F_000731"
     CDS             7563..8969
                     /gene="misS"
                     /locus_tag="ACFM6F_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC6164194.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9087..11513
                     /locus_tag="ACFM6F_000732"
     CDS             9087..11513
                     /locus_tag="ACFM6F_000732"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6164195.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDIPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFDSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFLLKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            11558..11803
                     /gene="rpsP"
                     /locus_tag="ACFM6F_000733"
     CDS             11558..11803
                     /gene="rpsP"
                     /locus_tag="ACFM6F_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC6164196.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11819..12328
                     /gene="rimM"
                     /locus_tag="ACFM6F_000734"
     CDS             11819..12328
                     /gene="rimM"
                     /locus_tag="ACFM6F_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC6164197.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12328..13077
                     /gene="trmD"
                     /locus_tag="ACFM6F_000735"
     CDS             12328..13077
                     /gene="trmD"
                     /locus_tag="ACFM6F_000735"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC6164198.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13093..13458
                     /gene="rplS"
                     /locus_tag="ACFM6F_000736"
     CDS             13093..13458
                     /gene="rplS"
                     /locus_tag="ACFM6F_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC6164199.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13478..13783
                     /locus_tag="ACFM6F_000737"
     CDS             13478..13783
                     /locus_tag="ACFM6F_000737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164200.1"
                     /translation="MPSEPSDGISYCRNIRNGTAPKSTPLPAHPESPSEPNKIYRLNV
                     IQYHISHITINALPPSLSKPQIIMTPSLLLSGLTFRLILALIAVSLLWGVYFWAVSA"
     gene            13780..14505
                     /locus_tag="ACFM6F_000738"
     CDS             13780..14505
                     /locus_tag="ACFM6F_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC6164201.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDVRTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14529..15404
                     /locus_tag="ACFM6F_000739"
     CDS             14529..15404
                     /locus_tag="ACFM6F_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC6164202.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15481..16395
                     /locus_tag="ACFM6F_000740"
     CDS             15481..16395
                     /locus_tag="ACFM6F_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFC6164203.1"
                     /translation="MKHPKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4292 a         5271 c         3621 g         3294 t
ORIGIN      
        1 ccgtctgaag ccgattcggt tttcagacga catcattatt tgacctgacg caaacagaaa
       61 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
      121 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
      181 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg ataaaggcga
      241 catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcactgtcg gcatcaatgt
      301 cgcctggtgc ggcatctgcg gtactgacct gcacgaattc atggaaggcc cgattttcat
      361 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtaa cgatgggaca
      421 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca aagtcggcca
      481 acacgtcgtg gtcgaaccct acatcatccg cgatgacgta ccgaccggag aaggcagcaa
      541 ctaccacctc tccaaagata tgaactttat cggcttgggc ggctgcggcg gcggtctgtc
      601 cgaaaaaatc gccgtcaaac gtcgttgggt gcatccgatt tccgacaaaa tcccgttgga
      661 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
      721 gaaagcaggc gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc tgttggctgc
      781 cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagtaaag cacgcaaaga
      841 caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt
      901 cgaagaggtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag
      961 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat
     1021 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagagct
     1081 ggatgtgcgc ggcacgattg cctactgtaa cgaccacgcc gaaaccatca aactggtcga
     1141 agaaggcaaa atcaacctcg aacccttcat cacccagcgc atcaagctgg acgaactggt
     1201 ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa ttattgtgag
     1261 tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac
     1321 ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
     1381 tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
     1441 caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc
     1501 ttgcataagg gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
     1561 atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt
     1621 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt
     1681 gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
     1741 tatattaaaa tccgcacttt cccacattca atccgtctga ccgcttttca gacggcatcg
     1801 gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
     1861 ccgtctatga agacggcgaa atggtttgtt tcaaagacat caaccccgcc gctccggttc
     1921 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc
     1981 agcccctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg
     2041 cagacggctt caaaaccctg atcaacaccg gaaaaggcgg cggacaagag gtcttccacc
     2101 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata
     2161 cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt
     2221 atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt
     2281 gcggttcatg acttcaaaca ggggctgaac gaaggtacag acggcaaaga agcccaaaaa
     2341 gacgatgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt
     2401 ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg
     2461 cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc
     2521 gtcaaacagg aatttgacac tcaaatcgaa ctggaagaac taaggaaggc aaagcaggaa
     2581 tttgaagctg ccgccgctca ggttcgagac agcctcaaag aaaccggtac ggatatgcag
     2641 aacagtctgc acgacatttc cgacggtctg aagccttggg aaaaactgcc cgaacagcgt
     2701 acgcctgctg atttcggtgt cgatgaaaac ggcaatccct ttcccgatgc ggcaaacacc
     2761 ctatcagacg gcatttccga cgttatgccg tccgaacgtt cctacacttc cgccgaaatc
     2821 cttggggaca gcgggcaaac cggcagtaca gccgaacccg cggaaaccga ccaagaccgc
     2881 gcatggcggg aatacctgac tgcttctgcc gccgcacccg tcgtacagac cgtcgaagtc
     2941 agctatatcg ataccgctgt tgaaacccct gttccgcata ccacttcgct gcgtaaacag
     3001 gcaataagcc gcaaacgcga tttgcgtccc aaatcccgcg ccaaacctaa attgcgcgtc
     3061 cgtaaatcat aaagagagca atccggtgtc cgaaacacaa aacgaacaac ccgtccaacc
     3121 gcttgtcgag catctcatcg agctgcgccg ccgcctgatg tggacggttg tcggcatctt
     3181 agtctgcttt ttcggcctaa tgccgtttgc ccaacaactc tatactttta tcgccgaccc
     3241 gctgatggca aacctgccca aagacaccag catgattgcc accgatgtca tcgcaccatt
     3301 tttcgtgccg gtcaaagtta ccctgatggc ggcattttta atttcgctgc cgcatacgct
     3361 ctaccaaatc tgggcatttg tcgcgcccgc actctatcaa aacgaaaaac gcctgattac
     3421 cccgcttgtc ctctcaagcg tcagcctgtt tttcatcggc atggcatttg cctactttct
     3481 ggtttttccc gtcattttca aattccttgc cagcgttacc cctgtcggtg tcaatatggc
     3541 gacagacatc gacaaatacc tatcttttat cttaggcatg tttgttgcgt tcggcacaac
     3601 gtttgaagtt cccattgtcg ttatcctgtt aaccaaaatc agcgtggtaa caaccggaca
     3661 gctcaaacgc gcccgcccct atgtgattgt cggcgcgttt gtcattgccg ccatcatcac
     3721 gccgcccgat gtgatttcac aaaccctgct tgccattccg ctgattctct tatacgaagc
     3781 aggtatttgg ttcggacgct ttttcacgcc acgttcagaa caggatggcg acatacagcc
     3841 gcctgcaaca acctgacact atgccgtccg aacctccgcc tcataccgca acagattaag
     3901 gaataccttt gaataccctc tatttaggtt caaacagccc gcgccgaatg gaaatcctga
     3961 cacagttggg ctatcaggtc gtcaagctgc ctgccgacat caatgaaacg gtcagacaga
     4021 acgaagaccc tgcccgttac gttcaaagga tggcagaaga aaaaaaccga accgccctga
     4081 ccctcttttg cgaaaccaac ggcacaatgc ccgatttccc cctgattacc gccgacacct
     4141 gcgtcgtttc agacggcatc atattgggca aaccccgctc ccaagctgaa gcaatcgaat
     4201 ttttaaaccg attgtccggc aaacaacata ccgtcctgac tgctgtctgc attcattatc
     4261 gcggcaaaac gtcaagccgc gtccaaacca accgcgtcgt tttcaagccc ctgagttcgg
     4321 aagaaatttc cgcctatgtg caaagcggcg aaccgatgga caaagccggc gcctacgccg
     4381 tacaaggcat aggcggcatc tttatccaat ctatcgaagg cagcttcagc ggcattatgg
     4441 gactgcccgt ttatgaaacc gtttcgatgt tgcaggattt gggataccgc tcccccttgt
     4501 ccgcccttaa accgtaaaga ccgccgtgaa cagacaaacc gcttacctcc ttacctcttt
     4561 cagcctgatc gcactgataa tcctgtccct ttcctgggaa ctgtggattg caccgttgcg
     4621 cccgggcggc tcgtggctcg cgctcaaagc cctccccctc tgtctgccgc tttcaggtat
     4681 cttgaaaaag aaaatctata cctaccaata cagctccatg ctggttctga tttattttgc
     4741 cgaagccgtt atgcgcctgt tcgacgccta tccagcagaa cagatttgcg ccgcgctttc
     4801 cgcagtattc agcatcatct tcttcatatc ctgcctgtcg ttcgtcaaac aatacaagga
     4861 aacaaacaat gcccgctgaa acgaccgtat ccggcgcgca ccccgccgcc aaactgccga
     4921 tttacatcct gccctgcttc ctttggatag gcatcatccc ctttaccttc gcgctcaggc
     4981 agcaaccgtc gcccgacttt taccacgatg ccgccgccgc agccggcctg attgtcctgt
     5041 tgttcctcac ggcaggaaaa aaactgtttg atgtcaaaat ccccgccatc agcttccttc
     5101 tgtttgcaat ggcggcgttt tggtatcttc aggcacgcct gatgaacctg atttaccccg
     5161 gtatgaacga catcgtctct tggattttca tcttgctcgc cgtcagcgcg tgggcctgcc
     5221 ggagcttggt cgcacacttc ggacaagaac gcatcgtgac cctgtttgcc tggtcgctgc
     5281 ttatcggctc cctgcttcaa tcctgcatcg tcgtcatcca gtttgccggc tgggaagaca
     5341 cccctctgtt tcaaaacatc atcgtttaca gcgggcaagg cgtaatcgga cacatcgggc
     5401 agcgcaacaa cctcggacac tacctcatgt ggggcatact cgcctccgcc tacctcaacg
     5461 gacaacgaaa aatccccgcc gccctcggcg taatctgcct gattatgcag accgccgttt
     5521 taggtttggt caactcgcgc accatcttga cctacatagc cgccatcgcc ctcatccttc
     5581 ccttctggta tttccgttcg gacaaatcca acaggcggac gatgctcggc atagccgcag
     5641 ccgtattcct taccgcgctg ttccaatttt ccatgaacac cattctggaa acctttactg
     5701 gcatccgcta cgaaactgcc gtcgaacgcg tcgccaacgg cggtttcaca gacttgccgc
     5761 gccaaatcga atggaataaa gcccttgccg ccttccagtc cgccccgata ttcgggcacg
     5821 gctggaacag ttttgcccaa caaaccttcc tcatcaatgc cgaacagcac aacatatacg
     5881 acaacctcct cagcaacttg ttcacccatt cccacaacat cgtcctccaa ctccttgcag
     5941 agatgggaat cagcggcacg cttctggttg ccgcaaccct gctgacgggc attgccgggc
     6001 tgcttaaacg ccccctgacc cccgcatcgc ttttcctaat ctgcacgctt gccgtcagta
     6061 tgtgccacag tatgctcgaa tatcctttgt ggtatgtcta tttcctcatc cctttcggac
     6121 tgatgctctt cctgtccccc gcagaggctt cagacggcat cgccttcaaa aaagccgcca
     6181 atctcggcat actgaccgcc tccgccgcca tattcgcagg attgctgcac ttggactgga
     6241 catacacccg gctggttaac gccttttccc ccgccactga cgacagtgcc aaaaccctca
     6301 accggaaaat caacgagttg cgctatattt ccgcaaacag tccgatgctg tccttttatg
     6361 ccgacttctc cctcgtaaac ttcgccctgc cggaataccc cgaaacccag acttgggcgg
     6421 aagaagcaac cctcaaatca ctaaaatacc gcccccactc cgccacctac cgcatcgccc
     6481 tctacctgat gcggcaaggc aaagttgcag aagcaaaaca atggatgcgg gcgacacagt
     6541 cctattaccc ctacctgatg ccccgatacg ccgacgaaat ccgcaaactg cccgtatggg
     6601 caccgctgct acccgaactg ctcaaagact gcaaagcctt cgccgccgcg cccggtcatc
     6661 cggaagcaaa accctgcaaa tgacccgcgc cggcggatgc ggataccgcc cgaaatgtaa
     6721 agctccatgc aagacattgc aaaaaacagc aaaccggtag ggaaatacgc tatcaaaaaa
     6781 cattgcagcc gtgttaagat aaaccgtcaa acaatctttt cacgccccgc ccgaaacagg
     6841 gtcggggcat acccttacga aaaggaaaca ccatgagccg cgtattactc gtagatgacg
     6901 atgccctgct aaccgaactg ctgaccgaat acctgagcgc cgaaggtctg aacgtccgca
     6961 gcgttcccga cggggaagca ggcgtacagg aaatcctgag cgggcagtac gatgtagtcg
     7021 tattggattc catgatgccc aaaatgaacg gcttggatgt cttgaaaaac gtacgcgccc
     7081 gaagcaccgt ccccatcatc atgctgaccg ccaaaggcga cgacatcgac cgaatcatcg
     7141 gcttggaaat gggcgcggac gactatgtcc ccaaaccctg cacaccacgc gaactcttgg
     7201 cacgcatcaa tgccatcctg cgccgcgcac aacacagcgg cgaacagaac aacgcaccca
     7261 acagcatctc cgtcagcgat gtcgtcctgt accccgccaa acgccaggca tccgtcaaag
     7321 acatgccgct cgaactgacc agcaccgaat tcaacctgct cgaagtcctg atgcgccatg
     7381 ccggacaggt agtcagcaaa gaaaccctgt ccgtcgaagc actcgaccgc aagctggcaa
     7441 aattcgaccg cagtatcgac gtacacatct ccagcatccg ccacaagttg ggcgatgcct
     7501 ctctgattca aaccgtacgc ggcttgggct acctgtttgt caaaaactga agcaacgggc
     7561 aaatgaaact gttccaacgc attttcgcca cattttgcgc agttatcgtc tgtgcaatct
     7621 ttgtggcgag tttttctttt tggctggtgc agaacaccct tgccgaaaac cagttcaacc
     7681 aacgccgcac catcgaaacc actttgatgg gcagcatcat ttccgcattc cgggcacgcg
     7741 gggacgcggg cgcgcgcgaa atcctgacgg aatggaaaga cagccccgtc tcatcgggcg
     7801 tgtacgttat acagggcgac gagaaaaaag atatcctgaa ccggtatatc gacagctaca
     7861 ccatcgaacg cgcccggctt ttcgccgccg gacacccgca ttccaacctc gtccatatcg
     7921 aatacgaccg cttcggcgaa gaatacctgt tcttcaccaa agactgggac aaactccaag
     7981 cccgccgcct gcccagcccc ctgttgatcc ccggtctgcc gctcgccccg atttggcacg
     8041 aactcatcat attgtccttc atcatcgtcg tcggactgct gatggcgtac atcctcgccg
     8101 gcaatatcgc caaacccatc agaatcttag gcaacggcat ggacagggtg gcaaacggag
     8161 aacttgaaac ccgtatctcc caacaggtcg acgaccgcga cgacgaattg tcccatcttg
     8221 ccatccaatt cgacaaaatg gtggaaaaac ttgaaaaact cgttgccaaa gaacgccacc
     8281 tgctccatca cgtctcccat gaaatgcgtt ctccccttgc gcgcatgcag gcaattgtcg
     8341 gactgattca ggcgcagccc caaaaacagg agcaatatct caaacggctg gaaggcgaac
     8401 tgacccgcat ggatacgctg gccggggaac tgttaaccct gtcccgtctc gaaacttcca
     8461 atatggcttt ggaaaaagaa agcctgaaac tcctgccctt cctgggcaac ctggtagaag
     8521 acaatcaaag cattgcccag aaaaacggac aaacggttac cctgtctgcc gacggaaaaa
     8581 tccccgaaaa tacaaccatc cttgccaacg aaagctacct gtaccgcgcc ttcgacaacg
     8641 tcatccgcaa cgccgtcaac tacagtcccg aaggcagcac catcctgatc aacatcggac
     8701 aagaccacaa acactggata atcgacgtta ccgacaacgg ccccggcgtg gacgaaatgc
     8761 agctcccgca catcttcacc gctttctacc gtgcagactc cagtgcgaac aaacccggaa
     8821 caggactggg gcttgcattg acccaacata ttattgaaca gcactgcgga aaaatcatcg
     8881 ccgaaaacat caaaccgaac ggtctgcgga tgcgcttcat cctgcccaag aaaaaaaccg
     8941 gttccaaaac agaaaaaagt gcgaactgac cataatgccg tctgaaccgg cttcagacgg
     9001 cattgcacaa acagttaccc caaaacaaac acacagcggg tgcatctcca acaatcacgc
     9061 acataccgtc cgcaaaggaa tatatcatgt cggcacaaac cgatccgggc tactttttca
     9121 tgcccaacca cattattctg ataggcgcga gcgaacgtcc gtacagcctg ggcgaacgcg
     9181 tactcagcaa cctgctgagt acgccctttc aaggaaaaat cacccccgta aacccgcgcc
     9241 accacaccat agccgggctt cccgcctaca ccagcctcaa caaaatcccc ggcaatgcag
     9301 acctgattat tgccgttacc ctacccgaca gttacgacac cctcttcaaa acctgccgca
     9361 aaaagcagct ccgacacatc atcctcatac aggactggga cagcctgtct gccgcagaac
     9421 tgcacaccgc cgaaactgcc atccgcaaac accacggcaa cggactcaac atcaccgcct
     9481 gcaccaccgc aggcatccaa ctgccctcac tcggactcaa catcagtacc caagacggat
     9541 acgccgcagg ccataccgcc atactgaccg gcaatgccgc cgtcagccgt caaatcgaca
     9601 acatcctgaa caaactccgt caaggcacat ctcgccacat cagcctgcat cccggcatca
     9661 gccccatcac atccgccgat tggctcaacc gcttcggaca cagcctgcac accaaaaccg
     9721 ccgtcctaca ccacaaccct gaagaggatc agcgcaaact gttcagcgca atccgccaat
     9781 ttacccgcca tacgccgctg attctccaca tcacctgccg cacgacagaa accgaccgtg
     9841 ccgtactgca ctgcctcgcc cgacactgca acttcctcat cagtttcaac gccgacgacc
     9901 tcgaagccgc actgcgcgcc caactgtccg acattccacc cctgtcccga ctcgacatcc
     9961 tgtccgacac gcctgccgaa tggctgaacg cgcacgcgcc aaaaaccctc gccctccact
    10021 ttcccaacct tccccaccac atccgcaacg gacacctgac cggcacaccc acaccctcaa
    10081 tctgccacga catcgcctca cgtcagcttg cccgccccga cacccaagcc gtcctgacca
    10141 tcctcagccc ctccggacac gaggattaca aaaaaacagc acgcgccctt atccgcctgt
    10201 ccgaacagac cgccaaaccc ctgctcgtca gcagcccctt ttcagacggc ataacccatt
    10261 tcgacacccc cactcaggca atccgcaccc tttcctaccg caacaccgcc gccgccctga
    10321 aacaggcaca gctcgacatt gcaccgccgc aaccgtgccg tctgaaaaca ccgcaacccc
    10381 aaaacatcaa aaaagccctt gcagcggcaa acccctccct gctcgccgaa gccctgcacc
    10441 tcccccccta ccggcacacc acccataacg ccgtacaatt ccaattcgac agccaccccc
    10501 tctatggcga catcctgacc gcacgctgca acggacaaac cactgccgta ctcccgccgt
    10561 ttaccacgct cgacagccgc caccttgccc gctttgccga actcgacggc acacaaaccc
    10621 tcgaccagtt cctgcacaca ctgaccgtca ttcccgaata ccgccaacac attctcggca
    10681 tcaccctcaa cctcaacggc ggacaataca gcagcgattt cctcttaaag acacccgaaa
    10741 cacacgacac gcccaaacgc aagaacacag gcaaagccgc ccaaaccctc gaacatgccg
    10801 ccgcaaaaat gcagagtgcc gccgcatacc tgaaacacaa aaacccgaca gccgccgaat
    10861 ttctccgcca cacaagcgaa gccgccgcag aactgctcgg cagcaaaacc gaaaccggag
    10921 cagccgtacc caacgtactt gccccctatc ccgcagcata ccccaaaaca ctgtccctaa
    10981 gaaacaacac gaccgttacc attaccccca ttttgcccga agacgcagaa gccaaacagc
    11041 agttcgtccg cagcctcggt cccgaagcac ggtacacacg cttcatgacc cacaccaacg
    11101 aactgcccgc agccacgttg gcacgcctgt gcaaccccga ttaccactgt gaagccgcat
    11161 ggacggcaaa ggatgccgac agcaacatcg tcgccgtcgt ccgccacagc cgcctgaatc
    11221 gcaacgaatg cgaattcggc atcacactgg cggaacatat gcgcggcagc ggactggcac
    11281 agaaaatgat ggaactcatc atccaaaccg ccgcacagca aggctaccgg actatgagcg
    11341 ccgacattct caaaaccaat acccccatga tcaaacttgc tgaaaaatca ggatttaccc
    11401 tcaaggaatc ggacaccgaa aaaaacctgt accgcgcata tctgaacctt gcggcagaca
    11461 aaacaacaga aaaaacaaat aaaaacttgc acaccgacca caaaataacc taaaatcggc
    11521 agtttccata tatccgattt tctcaaaagg actcaaaatg gtagttatcc gtttggcacg
    11581 cggcggctcg aaacaccgcc ccttctacaa cgtcatcgtt actgactcac gcagccgccg
    11641 cgacggccgc ttcatcgaac gcgtaggctt ctacaacccc gtagccaacg aaaaacaaga
    11701 gcgcgtccgc ctcaatgcag accgcctgaa ccactggatt gcacaaggcg cgcaagtcag
    11761 cgactccgtt gcaaaactga ttaaagaaca aaaagccgcc taatccacat ttgccgccat
    11821 gacagacact caaaaccggg tagccatggg ctacatcaaa ggcgtattcg gcataaaggg
    11881 ctggctgaaa attgccgcca acaccgaata ttccgacagc cttttggact accccgagtg
    11941 gcatttggtc aaggacggca aaaccatcag cgttaccctt gaagccggaa aagtcgtcaa
    12001 cggcgaactc caagtcaaat tcgaaggcat aaacgaccgc gacttggcat tctcattgcg
    12061 cggttacacc atcgaaatac cccgtgaagc attcgccccg acagaagaag acgaatacta
    12121 ctggacagac ttggtcggca tgaccgtcgt caacaaagac cataccgttt taggcaaggt
    12181 aagcaacctg atggaaaccg gcgcaaacga cgtattgatg attgacggag aacacgggca
    12241 gattctgatt ccgttcgttt cccaatatat cgaaaccgtc gataccggca gcaagattat
    12301 tactgccgac tggggtttgg actactgatg cttatccagg cagttaccat tttccccgaa
    12361 atgttcgaca gcattacccg ctacggcgta acgggacgcg cgaacagaca gggaatttgg
    12421 cagtttgaag cagtcaatcc ccgaaagttt gccgacaaca gattgggcta catcgacgac
    12481 cgcccgttcg gcggcggccc gggaatgatt atgatggctc cgccgcttca tgcagcaata
    12541 gaacacgcga aagcacaatc ttcccaaacc gcaaaagtca tctacctcag cccccaagga
    12601 aaaccgctga cacaccaaaa agcggcagaa ctggcagaac ttccgcatct gattctgctg
    12661 tgcggacgct atgagggaat agacgaaaga ctgctgcaaa ccagcgtcga tgaagaaatc
    12721 agcatcggag acttcgttgt ttccggcgga gagcttcccg ccatgatgct gatggatgcg
    12781 gtattgaggc tcgtacccgg cgtattgggc gatatgcagt ctgccgaaca ggattcgttc
    12841 tcaagcggca ttttggactg cccccactac accaaaccct tagaatttca aggtatggct
    12901 gttccggaag tattgcgttc cggcaatcat ggcttgatag cggaatggcg gttggaacaa
    12961 tcgctgcgcc gcaccttgga gcgcagaccc gatcttttgg aaaagcgcgt tttaatccca
    13021 aaggaatccc gcctcttaga aaccatccgg caagagcaac gggaaatcca atcataattt
    13081 aggaaaaaaa caatgaacct gattcaacag ctcgagcaag aagaaattgc ccgcctgaat
    13141 aaagaaatcc ccgaattcgc accgggcgac accgtagtcg tatccgtacg cgtcgtggaa
    13201 ggtacccgca gccgtctgca agcctacgaa ggcgttgtca ttgcacgccg caaccgtggt
    13261 ctgaacagca acttcatcgt ccgcaaaatc tccagcggcg aaggcgttga acgtactttc
    13321 caactgtact ctccgaccgt cgaaaaaatc gaagtcaaac gtcgtggcga cgtacgccgt
    13381 gccaaactgt actacctgcg cggtctgacc ggcaaagctg cacgcatcaa agaaaaactg
    13441 cctgcacgca aaggttgatt caaacccgtt ttccccaatg ccgtctgaac cttcagacgg
    13501 catttcttat tgccgcaaca tccgcaacgg aacagcacca aaaagcacac cccttccggc
    13561 acatcccgaa tccccgtccg aaccgaacaa aatttacaga ctaaacgtta tacagtatca
    13621 tatcagccat ataacgataa acgctttacc gccttccctt tcaaaaccgc aaatcatcat
    13681 gacaccttcc cttttactat caggattgac cttccgcctc atccttgccc tgattgccgt
    13741 atccctttta tggggcgttt acttttgggc agtatccgca tgagcatcat tgtcgaaaac
    13801 ctgaccgtca gctaccgccg ccgacctgcc gtgcaccatg tggacattac ttttgaagaa
    13861 catagtatgt gggcggtttt cggtcccaac ggcgcgggga aatccacctt tctcaaatcc
    13921 ttaatgggat tgcagcctat cgatacgggc agcatccggc tggacggatt gacccgtcag
    13981 aacattgcct accttcccca gcagtccgac atcgaccgct cgcagcctat gaccgttttc
    14041 gacttggcgg caatggggct gtggtatgaa atcggttttt tcaaagggat aaataccgct
    14101 caaaaacaac gcgttcacga agccttggag cgtgtaggaa tgcggcagtt tgccggccgc
    14161 caaattgccc acctttccaa cggacaattc cagcgtgtcc tttttgcccg aatgctggtt
    14221 caaaacgcca aattcctact tctggacgaa cctttcaacg ctgttgatgt acggacaacc
    14281 tacgagcttc tcgacgtgtt gcaaaaatgc cattgcggcg gacacgccat catcgccgta
    14341 ctgcacgatt acgaacaagt ccgtgcctac tttcccaata ccctgctgct cgcccgagaa
    14401 aaaattgcgg caggcgcaac cgagaccatt ctgacagaac cctacctcgc ccaagccaac
    14461 gccaaaatgc agcaacagga aagccccgag tggtgcgcgt cataaatgcc gtctgaaacc
    14521 gaaaaaccat gaacctctac gacctgctcc ttgccccctt tgcagaattt gactttatgc
    14581 gctacgccct cgcatccgtc ttctgcctgt ccctcagtgc cgcacccgtc ggcgtattcc
    14641 tcgtcatgcg ccgtatgagc ctgataggcg acgcattgag ccacgccgtc ttgcccggtg
    14701 ctgccgtcgg ctacatgttt gccggcttaa gcctgcccgc catgggtttg ggcggcgtag
    14761 ccgcaggtat gctgatggca ctgcttgccg gactcgtcag ccgcttcacc accctgaaag
    14821 aagatgccaa ctttgccgcc ttttatctca gcagcctcgc catcggcgta gtcctcgtca
    14881 gcaaaaacgg gagcagcgtc gatttgctcc acctcctttt cggctctgta cttgccgtcg
    14941 atattcctgc cctgcagctc atcgccgccg tctccagcct cacgctcatt acccttgccg
    15001 tcatctaccg cccgctcgta ctcgaaagca tcgaccccct gtttctcaaa tctgtcggcg
    15061 gcaaaggcgg gctttggcac gtcctctttc tcgtcctggt cgtcatgaac ctcgtatccg
    15121 gctttcaagc cctcggcaca ctcatgtccg tcggacttat gatgctgcca gccattaccg
    15181 cccgcctatg ggcgaagcac atgggcgcac tcatcctcct atccgttctg acagccctgc
    15241 tgtgcggctt gagcggactg ctcatttcct accacatcga aattccttcc ggtcccgcca
    15301 tcatcctctg ttgcagcgtc ctttatctct tttccgtcat actcggcaaa gaaggcggca
    15361 ttctgaccaa atggctcaaa aaccaccgcc accacaccac ctgaccccgc aagccgcttg
    15421 aacaaacagg cggcttgttt taccataaca gatgttacgt tatatcacag gagaacaatt
    15481 atgaaacacc ccaaactcac ccttatcgcc gcattgctga ccactgccgc aactgccgcc
    15541 cccctgccgg ttgtaaccag cttcagcatt ttaggcgacg tagccaaaca aatcggcgga
    15601 gagcgcgtat ccatacaaag tttggtcgga gccaaccaag atacgcacgc ctatcatatg
    15661 accagtggcg acattaaaaa aatccgcagt gcaaaactcg tcctgctcaa cggcttagga
    15721 cttgaagctg ccgatgtaca acgtgccgtc aaacagagca aagtatccta tgccgaagcg
    15781 accaaaggca tccaacccct caaagccgaa gaagaaggcg gacaccatca cgaccacgat
    15841 catgaccacg aaggacacca ccatgaccac ggcgaatatg acccccacgt ctggaacgac
    15901 cccgtcctta tgtccgccta tgcccaaaac gttgccgaag ccctgataaa ggccgatccc
    15961 gaaggcaaag tttattatca acaacgcttg ggcaactacc aaatgcagct taaaaaactg
    16021 cacagcgacg cacaagccgc atttaacgcc gtccctgccg ccaaacgcaa agtcctgacc
    16081 gggcacgatg ccttttccta tatgggcaaa cgttaccata tcgaattcat cgccccgcaa
    16141 ggcgtgagca gcgaagccga gccttcagcc aaacaagtcg ccgccatcat ccgccaaatc
    16201 aaacgcgaag gcatcaaagc cgtatttacc gaaaacatca aagacacccg catggttgac
    16261 cgtatcgcca aagaaaccgg tgtcaacgtc agcggcaaac tgtattccga cgcactcggc
    16321 aacgcgcccg cagacaccta catcggaatg taccgccaca acatcaaagc cttgaccaac
    16381 gcgatgaagc aataaaaccg tttgattggt ttgaatttga aaaggcaaaa ggccgtctga
    16441 aaattatagt ggattaacaa aaatcaggac aaggcgac
//
LOCUS       DAXHPG010000031        16325 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXHPG010000031 DAXHPG010000000
VERSION     DAXHPG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.031"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..311
                     /locus_tag="ACFM6F_000741"
     CDS             <1..311
                     /locus_tag="ACFM6F_000741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164204.1"
                     /translation="DTTLVLVSVISSFFSSREYIDSRRQVLDTAFSYKSDGRMVLFKS
                     IKQESISFLSEDLKKDWMFHKVKTGEKDGYGSDEMLSVPRVYLEMMSRKTGVPYSSIL
                     "
     gene            complement(758..1120)
                     /locus_tag="ACFM6F_000742"
     CDS             complement(758..1120)
                     /locus_tag="ACFM6F_000742"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFC6164205.1"
                     /translation="MKAARRQLYYTFANRIAEQSRIICIKKLNLLNLQCIQIEVNKLH
                     QKIVAPSPLLHTLKEGVAKNGVHIEEIKANATSQSCYSCGRLNKRIGVAATRTNMVCE
                     GFGRHYDTDENASSNIRK"
     gene            1493..2920
                     /locus_tag="ACFM6F_000743"
     CDS             1493..2920
                     /locus_tag="ACFM6F_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6164206.1"
                     /translation="MKISRPLEFTLLQQEYMQHLTERMTQIAKLLNSSANNPDIDIPD
                     FLTEIKDYSEFSVTDENGTYLHWDKFRQIHTEDTRMKWRAVKESRKKIQKPIDFPFEH
                     QFWFCIPDSLQARLHLIDKSCGSSIGTSSLGGFGRSEQNRFLLKSLIMEEAITSAQLE
                     GAATTRKVAKDMLKSQRKPKTKDEIMIVNNYHLMKKAVELKNTPLSVEMILDLHRIAT
                     SNAIENKADPGQFRRDDEIFIADNDGNSLYQPPPHGQVHTLMEAVCTFANNTYDGVEN
                     PFIHPVVQAIILHFLIGYIHPFGDGNGRIARALFYWFMLKNSYWLFEYISISRLLKNA
                     PAQYAKSYLYAETDDSDLTYFIYYQCDIIKRAVADLEHYISDKQKHQQEFKAAIAQYT
                     EKIGKLNQRQIGILQKAVEESGKIFTALEIANQYGISLNTARSDLSKLGEYRFLVPFK
                     SGNALEYVAPQDLLERLEKKNSLLA"
     gene            3309..3773
                     /locus_tag="ACFM6F_000744"
     CDS             3309..3773
                     /locus_tag="ACFM6F_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164207.1"
                     /translation="MLHIPPTEPDRYVSSIVALNVHSSNGTGDWHSAAALNDDAYPQD
                     FYIYGKDQTRSTLHLLGNVGVIDGTNRFNEMGYYPENIPVWLADHPRACVDYLYTAVL
                     QTGSIGRVILDDWFPSDEDKQSVYDLLNQIEPHLNTQEWENLQLWKRKNPIM"
     gene            3900..5189
                     /locus_tag="ACFM6F_000745"
     CDS             3900..5189
                     /locus_tag="ACFM6F_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFC6164208.1"
                     /translation="MMKSILTVSGNRMRKPRITHLDVWANDERIGTLEKGAMYRFTYD
                     NPGSPLLGLHYQDRGKVYISNNMPHIFAQYFPEGFLDAHITSKYAFHDAPFEDNEMLR
                     LAILGRETLGRIHVRCNDPLFNEWIDGLEMKNPRILTERDLLGINARQVFQQYMAEIF
                     HHGRFVSVSGIQQKMSLDAISRNTKQTASYIAKGFDASEYPCLAANEFLCMQTIKQAG
                     IPVAQTSLSEDSSVLLVRRFDVSEQEYFLGMEDFTSLRQYSAEDKYKGSYAAIAQIIR
                     QISGRPDEDLIHFFNQLAASCILKNGDAHLKNFSVLYHDEYDVRLAPTYDVLDTSIYR
                     VGTQGIFDAYDDTLALNLTNHGKKTYPSKNTLLDFAEKYCDLGREDASFMIDTIVQAK
                     EQVLVKYSDVLRENEWLAQKWHFIPDENEEGLPFTFR"
     gene            complement(5526..7313)
                     /locus_tag="ACFM6F_000746"
     CDS             complement(5526..7313)
                     /locus_tag="ACFM6F_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFC6164209.1"
                     /translation="MKLPLSYLPNIRFLSWCCLLAGIIAPATLLASPNPAEIRMQQDI
                     QQRQREEQLRQTMQPESDVRLHQKNTGETVNQLMGDDSSQPCFAINEVVLEGEHHDRF
                     QFALKRALRETGFQAGKCLHAGNINQIMSLAQNALIGRGYTTTRILAAPQDLNSGKLQ
                     LTLIPSYLRSIRIDRSNDDQTHAGRIAAFQNKFPTRSNDLLNLRDLEQGLENLKRLPT
                     AEADLQIVPVEGEPNQSDVVVQWRQRLLPYRVSVGMDNSGSEATGKYQGNITFSADNP
                     FGLSDMFYVNYGRSIGGTPDEESFDGHRKEGGSNNYAVHYSAPFGKWTWAFNHNGYRY
                     HQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLGVKLWTRETKSYIDDAELTV
                     QRRKTAGWLAELSHKEYIGRSTADFKLKYKRGTGMKDALRAPEEAFGEGTSRMKIWTA
                     SADVNTPFQIGKQLFAYDTSVHAQWNKTPLTSQDKLAIGGHHTVRGFDGEMSLSAERG
                     WYWRNDLSWQFKPGHQLYLGADVGHVSGQSAKWLLGQTLAGTAIGIRGQIKLGGNLHY
                     DIFTGRALKKPEFFQSRKWASGFQVGYTF"
     gene            complement(7368..7826)
                     /locus_tag="ACFM6F_000747"
     CDS             complement(7368..7826)
                     /locus_tag="ACFM6F_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFC6164210.1"
                     /translation="MPIISTLNILSPKLYPSEPYNESEALGAITWLWYQSPTHRQVPI
                     VEMMTYILPVLKNGQFALFCKGTQPIGYISWAYFDEVAQAHYLESDRHLRDNSDWNCG
                     DNIWLIQWFAPLGHSHQMRSAVRQLFPSTTVRALYHKGSDKGLRILTFKT"
     gene            complement(7992..8327)
                     /locus_tag="ACFM6F_000748"
     CDS             complement(7992..8327)
                     /locus_tag="ACFM6F_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164211.1"
                     /translation="MKQILFKYFMLITCVFGLSSCGYLLSFGQLMFDGSCADWNCSKP
                     TPRWRKAVDDCENRNLEIIKQRFGYKDYAEVIFSEVGQQIQIPYQCRQSNGGYDIRKD
                     KQYGHLFVQ"
     gene            complement(8308..15837)
                     /locus_tag="ACFM6F_000749"
     CDS             complement(8308..15837)
                     /locus_tag="ACFM6F_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6164212.1"
                     /translation="MNKTLYRVIFNRKRGAVVAVAETTKREGKSCADSDSGSAHVKSV
                     PFGTTHAPVCRSNIFSFSLLGFSLCLAVGTANIAFADGIIADKAAPKTQQATILQTGN
                     GIPQVNIQTPTSAGVSVNQYTQFDVGNRGAILNNSRSNTQTQLGGWIQGNLWLARGEA
                     RVVVNQINSSHSSQMNGYIEVGGRRAEVVIANPAGIAVNGGGFINASRATLTTGQPQY
                     QAGALTGFQVRSGHIAITGQGLDARDTDFTQILSHAVKIDGPVWGKDVRVSAGKNDVS
                     ADGSIQSSHSPATNINSDNSLYAIDTGALGGMYAGKITLISTDRDASVRNQGQWFASA
                     GNVAIDANGRLVNSGTMAAANAKDTDNTAEHKVNIRSQGVENSGTAVSQQGTQIHSQS
                     IQNTGTLLSSGEILIHNSGSLKNETSGTIEAARLAIDTDTLNNQGKLSQTGSQKLHID
                     AQGKMDNRGRMGLQDTAPTASNGSSNQTGNSYNASFHSSTTTPTTATGTGTATVSISN
                     ITAPTFADGTIRTHGALDNSGSIIANGQTDVSAQQGLNNAGQIDIHQLNAKGSAFDNH
                     NGTIISDAVHIQAGSLNNQNGNITTRQQLEIETDQLDNAHGKLLSAEIADLAVSGSLN
                     NQNGEIATNQQLIIHDGQQSTAVIDNTNGTIQSGRDVAIQAKSLSNNGTLAADNKLDI
                     ALQDDFYVERNIVAGNELSLSTRGSLKNSHTLQAGKRIRIKANNLDNAAQGNIQSGGA
                     TDIGTQHNLTNRGLIDGQQTKIQAGQMNNIGTGRIYGDNIAIAATRLDNQDENGTGAA
                     IAARENLNLGIGQLNNRENSLIYSGNDMAVGGALDTNGQATGKAQRIHNAGATIEAAG
                     KMRLGVEKLHNTNEHLKTQLVETGREHIVDYEAFGRHELLREGTQHELGWSVYNDESD
                     HLRTPDGAAHENWHKYDYEKVTQKTQVTQTAPAKIISGNDLTIDGKEVFNTDSQIIAG
                     GNLLVQTEKDGLHNEQTFGEKKVFSENGKLHSYWREKHKGRDSTGHSEQNYTLPEEIT
                     RNISLGSFAYESHRKALSHHAPSQGTELPQSNGISLPYTSNSFTPLPSSSLYIINPVN
                     KGYLVETDPRFANYRQWLGSDYMLDSLKLDPNNLHKRLGDGYYEQRLINEQIAELTGH
                     RRLDGYQNDEEQFKALMDNGATAARSMNLSVGIALSAEQVAQLTSDIVWLVQKEVKLP
                     DGGTQTVLVPQVYVRVKNGDIDGKGALLSGSNTQINVSGSLKNSGTIAGRNALIINTD
                     TLDNIGGRIHAQKSAVTATQDINNIGGILSVEQTLLLNAGNNINSQSTAASSQNTQGS
                     STYLDRMAGIYITGKEKGVLAAQAGKDINIIAGQISNQSEQGQTRLQAGRDINLDTVQ
                     TGKHQATHFDADNHVIRGSTNEVGSSIQTKGDVTLLSGNNLNAKAAEVSSANGTLAVS
                     AKNDINISAGINTTHVDDASKHTGRSGGGNKLIITDKAQSHNETAQSSTFDGRQVVLQ
                     AGNDANILGSNVISDNGTRIQAGNHVRIGTTQTQSQSETYHQTQKSGLMSAGIGFTIG
                     SKTNTQENQSQSNEHTGSTVGSLKGDTTIVAGKHYEQIGSTVSSPEGNNIIHAQSIDI
                     QAAHNKLNSNTTQTYEQKGLTVAFSSPVTDLAQQAIAVAQSSKQVGQSKNGRVNAMAA
                     ANAGWQAYQTGKSAQNLANGTTNAKQVSISITYGEQQNRQTTQVQASQAQASQIQAGG
                     KTTLIATGAAEQSNINIAGSDIAGKAGTILIADNDITLQSAEQSNTERSQNKSTGWNA
                     GAAVSFGQGGWSLGVTAGGNVGKGYGNGDSVTHRHSHIGDKGSQTLIQSGGDTTIKGA
                     QVRGKGVQVNAKNLSIQSVQDRETYQSKQQNASAQVTVGYGFSASGDYSQSKIRADHA
                     SVTEQSGIYAGEDGYQIKVGNHTDLKGGIITSSQSAEDKGKNRFQTATLTTSDIQNYS
                     QYQGKSFGLGASVAVSGKTLGQGAQNKPQNKHLTSVADKNGASSSVGYGSDSDSQSSI
                     TKSGINTRNIHITDEAGQIRLTGKTAAQTKADIDTNVTTDTAERHSGSLKNTFNKEAV
                     QSELDLQREVTQEFDKTRQGVKQELYAVVDSKREQATKERLKNGGYDNDKSRALNKEA
                     NELDEKIRWLDAGLGLVWGAGSSDMAWSMFATTQADRAVRSATAPKEMWFHKKIIDEK
                     TGKVSFDTRQIWSLNDLSKEELASIQDTNGKVITVSNPGIFNNREDSLSNAAKQNRNS
                     TNGSGVIAVMNPPTGKYKSDSNNKIKDSLWLSSSLVSELMYVGYDQLNNKVFQGYLPK
                     TNSEKLNQDIYREVQKMDNGWSVDTSNHSRGGITASVSLKDWVNNKEQNGIAPIRKAR
                     FYGTATNVKNDYADVLQKNGYTYTGADGKTYNSGAYSIVHDKDFVGNKWIPFLLGTNN
                     TTQGACKGLCYSHSSYFAEVPEQYQRDENGNIKMENGKPVETENWEDYTKKWGIPKTG
                     ADGKYINHAIPKLVNPNNPNGEKYETNPF"
     gene            15993..16145
                     /locus_tag="ACFM6F_000750"
                     /pseudo
     CDS             15993..16145
                     /locus_tag="ACFM6F_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4157 a         3518 c         3553 g         5097 t
ORIGIN      
        1 cggatacgac cttagttttg gtatccgtaa tatcatcatt tttttcgtca agggagtata
       61 tcgactctag aagacaggta ttagatactg ccttttctta caagagtgat ggtaggatgg
      121 ttctcttcaa gtcaatcaaa caggaaagta tttcttttct gtctgaagat ttgaaaaagg
      181 actggatgtt tcacaaggtt aaaactgggg aaaaagatgg atatggttca gatgaaatgc
      241 tgagcgtacc ccgtgtctat ttggaaatga tgtcgcggaa aacgggagtc ccctactcca
      301 gtattcttta aagtctaagt agaagacttc ttcgtcggtc tttttttgtt gtttggtttg
      361 catggagtaa aactgtacaa ctgttgaagc ataaaaccag caagaaggta aaaaagaaag
      421 aagcagttct ttggatttta gatgttaccg caattagttt cctttcctaa aatttgttta
      481 aattatttgc aatattaata taaacgagat attaatgatg agaaatcaaa aaggcatact
      541 aaatacaatt tgtacaaaat aattgcagta tttaaaaatg ttgattcgta tatgaaaagt
      601 taaaaatgcc aaaatgtaca gttgctaaac tgtaaaactg ctaaagcaac aaaacataaa
      661 aaggaatgca gggatgcaat cactacatct ttttattccg taagcattta tgactttacg
      721 gtcaactgct actctatgtg cctagctttt cagctcccta ttttcgaata ttggacgagg
      781 cattttcatc agtgtcgtaa tgccggccga aaccctcaca aaccatattg gttcttgtgg
      841 cagcaacacc tatccgtttg ttcaagcgac cacaagagta acatgattgg ctggtggcat
      901 tggctttaat ctcttcgata tgaactccat ttttagctac accttctttc agcgtatgca
      961 acagtgggga tggggcaact atcttctggt ggagtttgtt gacttcaatc tgtatgcact
     1021 gtaggttgag cagattcagt tttttgatac agattatccg gctttgttcg gcaattctgt
     1081 tggcgaacgt atagtagagc tgtcgtcttg ccgctttcaa tttgcggtag gtattttacc
     1141 attccatacg tagccgctcg gtacgtttga gccaaatggt atcttcgcca tttgtaccaa
     1201 ttggttttta cattaggctg tgttttagta atttattaat ttcaattatt tgcaagggaa
     1261 aagacaatta ttttccggtt aggaataaac ctatcctgtt gaatacctta aagccaaata
     1321 cgcctatcaa caccatatta aaacacagcc ttttttaata tagtagacac aatctttccc
     1381 tatttatgaa ggtgatcgct tctttctgat tcgtatttta atgcttccta tttttataaa
     1441 aattgactag aatagctcaa ttattaaaaa ttgcgcgatt ttggtattta tcatgaaaat
     1501 ttccagacct ctggaattta ccctgttgca acaggaatat atgcagcatc tgactgaaag
     1561 aatgactcaa attgccaagc tgctgaattc ttccgcaaac aatcctgata tagacattcc
     1621 cgattttctt actgaaatca aagattattc agaattttcc gtgacagatg aaaatggaac
     1681 ctacctgcat tgggacaaat tccgccagat tcacacggaa gatacgcgga tgaaatggcg
     1741 cgccgttaag gaaagccgca aaaaaatcca aaaaccaatt gatttcccgt ttgaacatca
     1801 gttttggttc tgcattcccg actctttgca ggcacggctt catttgattg acaaaagctg
     1861 cggcagttct atcggcacgt ctagcttggg tggcttcggc agaagcgagc aaaacagatt
     1921 cttgctcaag tctctgatta tggaagaagc gattacatcc gcccaactgg aaggtgcggc
     1981 taccacgcgt aaagtggcca aggatatgct caaatcgcag cgtaaaccca aaacaaaaga
     2041 cgaaatcatg atagtgaaca actatcactt gatgaaaaaa gcggtagaat tgaaaaatac
     2101 gccgttaagt gttgaaatga ttttggattt gcaccgcatt gctaccagca acgctattga
     2161 aaacaaggcc gaccccgggc aattcaggcg ggacgacgaa atctttatcg ccgataacga
     2221 cggcaacagc ctgtaccaac caccgccaca cgggcaggtt catacgctga tggaagcggt
     2281 atgcacgttt gccaacaaca cttatgacgg cgtggaaaat ccgtttatcc atccggttgt
     2341 ccaagctatc atcctgcatt tcctgattgg ctacatccat ccgttcggag atggcaacgg
     2401 gcggatagcg cgggctttgt tctattggtt tatgctcaaa aacagctact ggctgtttga
     2461 atacatatca atcagccgtc ttctgaaaaa cgctccggcc caatacgcca aatcctattt
     2521 gtatgcggaa actgacgatt cagatttaac ctatttcatc tattaccaat gcgatattat
     2581 caagcgggcg gttgccgatt tggagcacta catttccgac aaacaaaaac accaacagga
     2641 attcaaagca gcgattgccc aatatactga aaagatagga aagttgaacc aacggcaaat
     2701 tggtatcctg caaaaagcag tggaagaaag cggaaaaatc tttactgcac tagaaattgc
     2761 caaccaatac ggcatctccc tgaatactgc ccgtagcgat ttgagcaaac tgggagaata
     2821 tagattccta gtaccgttca aatcaggaaa tgctttagag tatgttgctc ctcaggattt
     2881 attggaaagg ttagaaaaaa aaaatagttt gctagcctaa aatgcagctt taaccgagtc
     2941 aaaatcaatg cagtccgcac ctttaaaaag aagctgcgga ctgcttgctt tttgctctac
     3001 aaatgatctt tgtagctgat ttaaccaaga ttgtagcaat tttgctttcc aagcaagcag
     3061 ggttagaaaa ttcgatactt ttaattattg gctgtgtttt aatatgatgt tgataggcgt
     3121 gtttggcttt aaggtattca acaggatagg tttattccta accggaaaat aattgtcttt
     3181 tcccttgcaa gtaattgaaa tcaacagatt actaaaacac agccttacat tattggggtg
     3241 actatcctgt aaaatatgtc ctaaaacgtg gaaaccactt ttgctctgct aaattttaag
     3301 gaatctttat gttacatata cccccaacgg aacctgatcg atatgttagc agtattgtag
     3361 ccttaaacgt gcatagttct aacggtacag gcgattggca cagtgcagca gcccttaatg
     3421 atgatgctta cccccaagat ttctatattt atggcaaaga tcaaacacgt agcacgttac
     3481 atttgcttgg taacgttggt gtgattgatg gaacaaaccg cttcaatgag atgggctatt
     3541 atcctgaaaa catcccagtt tggctcgcag atcacccccg tgcttgcgtg gattatcttt
     3601 acacagcagt gttacaaact ggctcaatcg gtcgggtgat tttagatgat tggtttccaa
     3661 gcgatgaaga caagcaatca gtttatgact tacttaacca aatcgaaccg cacttgaata
     3721 ctcaagaatg ggagaattta cagctatgga aacgcaaaaa cccaataatg taatgctaac
     3781 cgaacgagat aaaaggcacg agaagcggta ttactgaatt tcatccgcaa tacgccatgt
     3841 acaattaaat cagcagatgt gtctaaacgt accgctgttc ccgtttgaaa gaaattttca
     3901 tgatgaagtc tatcctcacc gtatccggaa atcgtatgcg taaacccaga atcacccatt
     3961 tggatgtttg ggcaaacgat gaaagaatcg gtactttgga aaagggggca atgtaccgtt
     4021 tcacatatga caaccccggt tctccattgt tgggtttgca ctatcaggat aggggtaaag
     4081 tatatatcag caacaacatg ccgcatatct ttgcacagta ttttccggaa ggctttttgg
     4141 atgcacacat cacaagcaaa tatgcttttc atgatgcgcc ttttgaagac aatgagatgc
     4201 tgcgcttggc aattctgggc agagagactt tgggtcggat acatgtgcgc tgtaatgacc
     4261 cgctttttaa tgaatggatt gacgggttgg agatgaaaaa tccaagaata ttgactgaac
     4321 gggatttgct gggcataaat gcccgacagg tttttcagca atatatggca gaaatcttcc
     4381 atcacggtcg tttcgtcagt gtatccggga tacagcagaa gatgtcctta gatgccatca
     4441 gcagaaatac caagcaaact gcctcatata ttgccaaagg ttttgatgca tccgaatatc
     4501 cttgcttggc tgccaatgaa tttttatgca tgcagaccat caaacaagca ggtattccgg
     4561 ttgcacaaac cagcctgtcg gaagattcat cagtcttatt ggtacgtcgg tttgatgtca
     4621 gtgaacagga gtatttctta ggcatggaag actttaccag tctgcgtcag tattcagcag
     4681 aagataaata taaaggcagt tatgcggcta ttgcacagat tatccgacag atatccggca
     4741 ggcccgatga agatttaatc cattttttta accagcttgc cgccagttgc atattgaaaa
     4801 acggggatgc acaccttaaa aatttttcag tactctatca tgacgaatac gatgtacgtc
     4861 ttgcacctac ctatgatgta ttggatacat caatatacag ggttggaaca caaggaattt
     4921 ttgatgctta tgacgatacg ctggcattaa acctgactaa ccacggtaag aaaacatatc
     4981 cttccaagaa tacattgttg gattttgctg agaaatattg cgatttggga agagaagatg
     5041 catcctttat gatagataca atcgttcaag ctaaagaaca ggttcttgtt aaatactcgg
     5101 atgtattgcg tgagaatgaa tggttggcgc agaagtggca ttttatcccg gatgaaaatg
     5161 aagaaggtct accgtttaca ttccggtagc tgccgggttt gaaccggctg tcagagatgg
     5221 ccggtcgact ttcaccctga aaatcacttc atcttatggt gtttgaaacc gagaaattag
     5281 aagaatcgta ttcggtagga gatatactag gaagattgga aaactggtaa atcattctat
     5341 tgattgagtt ggcggcctat atgtttaatg taggcaaacg agaaggaatc atgtatttca
     5401 tgattccttc ttaaattcct gtgtcaatct aagatcaaaa cacagccatc tcttaccata
     5461 atcatgatag gtgttttatt atgaaaagct atatctataa ttaccgttga tttgactatg
     5521 ccgttttaaa acgtatagcc tacctgaaaa ccgcttgccc atttccttga ttggaaaaat
     5581 tcgggctttt tcaatgcgcg gccggtaaat atatcgtaat gcaggttgcc gccaagcttt
     5641 atctgcccgc gtatcccaat tgctgtgccg gctagagttt ggcccaataa ccatttggcg
     5701 gattgtcctg aaacatgtcc tacatcagcc ccaagataaa gctgatggcc tggtttaaat
     5761 tgccagctca aatcgttgcg ccaataccat ccccgctcgg cagacaaact catttcaccg
     5821 tcgaagccac gtacggtgtg gtgtccgccg atagccagtt tgtcttgcga tgttagcggg
     5881 gttttgttcc attgtgcatg aacggatgtg tcataggcaa atagctgttt accgatttga
     5941 aaaggagtat ttacatcagc cgatgccgtc caaattttca tacgtgacgt gccttctcca
     6001 aaggcttctt caggcgcgcg cagagcatct ttcatgccgg tgccgcgttt atatttcaac
     6061 ttaaaatctg ccgtactgcg gccgatatat tctttgtggg aaagttctgc caaccaaccc
     6121 gcagttttac gccgttgtac agtcagttcg gcatcatcaa tgtaactttt tgtttccctc
     6181 gtccacagtt ttacaccaag ataggttttg cgtttggcat cacgatacaa caggcggttg
     6241 aagccgaaat cagtattgta actttttcca ttatagtcat agacttccga taatccggaa
     6301 accgcctgat ggtaacggta gccattgtga ttgaatgccc atgtccattt accgaaaggg
     6361 gctgaataat gtacggcgta attgtttgat ccgccttctt tgcgatggcc gtcaaaactt
     6421 tcctcgtcgg gcgtaccgcc aattgaacgt ccataattta catagaacat atcactcagt
     6481 ccaaaaggat tgtcggcaga gaaagtgata tttccttggt attttcctgt cgcctcacta
     6541 cccgaattat ccatccccac actcacacgg tagggcagca gacgttgccg ccattgcacc
     6601 acgacatcac tttggtttgg ttctccctct acgggaacga tttggagatc ggcttccgca
     6661 gtcgggagac gtttgagatt ttccagtcct tgttccaaat cacgcagatt caacagatcg
     6721 ttcgagcggg tgggaaattt gttctggaat gctgcaatac gtcctgcatg ggtttgatca
     6781 tcgttagacc gatcgattcg tatggagcgc agatagctcg gtatcagggt taattgaagc
     6841 ttgccactat tcaaatcctg tggcgcagcc aagatacggg tcgtggtata tcccctgccg
     6901 atcaaagcat tttgtgctaa ggacatgatt tgattaatgt tgcccgcatg cagacacttg
     6961 ccagcctgaa aacccgtttc gcgcaaggca cgttttaggg caaactgaaa ccgatcatgg
     7021 tgttcacctt ccaacaccac ttcgttaatg gcaaaacacg gttggctgct gtcatcgccc
     7081 atcaactgat taaccgtttc ccccgtgttt ttttgatgca aacgcacatc gctttcaggc
     7141 tgcatggttt ggcgcaactg ctcttcgcgt tggcgttgct gaatatcttg ctgcatacgg
     7201 atttcggcag ggttggggga agccaacaaa gtagcaggag cgatgatacc tgccaataag
     7261 cagcaccaag acaaaaagcg aatattaggc aaataggata aaggaagttt catggcatgg
     7321 tcgcaaaatc aataaaatca tcaatgggca ttcaatacac gattgcatca agttttaaaa
     7381 gttaaaattc tcaaaccctt atcgctccct ttatgataca aggcgcgtac tgtcgtacta
     7441 ggaaataact ggcgcacagc tgagcgcatt tgatgactgt gtcccaatgg cgcaaaccat
     7501 tgaatcagcc aaatattgtc gccacagttc caatcgctgt tgtcgcgcaa atggcggtca
     7561 gattctaaat aatgcgcctg cgccacttca tcaaaataag cccatgagat ataaccgatt
     7621 ggttgggtac ccttgcaaaa caaagcgaac tgcccgtttt ttaacacagg caatatatac
     7681 gtcatcatct ccacaatagg tacttggcga tgcgtaggcg actgatacca tagccaagtg
     7741 atggcaccga gtgcttcgct ttcgttatat ggctcactgg gatagagctt gggtgagagg
     7801 atgtttaggg ttgagatgat gggcatattt taagttaggg ttggtgttgg ttaaaacaaa
     7861 aaatggtttc agcctgaaat gaaacatttc acgttaaaac atagctttag catacgagac
     7921 tgaattgtag ggggcaataa gcaacgaccc taccattcaa caattctcgt taattgcaca
     7981 ggttacgctg gttattgcac aaacaaatgc ccatactgtt tatctttacg aatatcataa
     8041 ccaccattac tttgcctaca ttgataaggt atttgaattt gttgtccaac ttcactaaaa
     8101 atcacctctg cgtaatcttt atacccaaat ctctgtttaa taatctccaa gtttctattc
     8161 tcacagtcat caactgcctt tctccatctt ggcgtaggtt tagaacaatt ccaatctgca
     8221 caactgccat caaacattaa ctgcccaaat gataataaat aaccacaact gcttaatcca
     8281 aaaacacaag taattagcat aaaatattta aaaaggattt gtttcatatt tttctccatt
     8341 cggattattg ggattgacta attttggaat agcatgattt atatatttac catcagcacc
     8401 tgttttaggg attccccatt tttttgtgta atcttcccag ttttctgttt caacaggttt
     8461 cccattttcc attttaatat tcccattttc atctctttga tactgctcag gcacttccgc
     8521 aaaataactg ctatgcgaat agcacaaccc cttacatgca ccttgtgtgg tgttattggt
     8581 tcctagcaag aaaggtatcc atttgttccc cacaaaatct ttatcatgca cgattgagta
     8641 ggctccgctg ttataagttt taccgtctgc acccgtatag gtatagccgt ttttctgtaa
     8701 aacatcggcg taatcattct tcacatttgt ggctgtacca tagaaacgtg cttttctgat
     8761 tggggcaatg ccattttgct ctttattgtt tacccaatct tttagggaaa cgcttgctgt
     8821 aattccccca cgactgtggt tactggtatc aacagaccag ccattatcca ttttttgaac
     8881 ctctcgataa atatcttgat tcagtttttc tgaattggtt ttgggtaaat agccttggaa
     8941 cactttatta tttaattggt cgtaaccgac atacatcagt tcagaaacaa gacttgaact
     9001 gagccataaa gaatctttta ttttgttatt agaatcagat ttatatttcc ctgttggagg
     9061 attcataact gcaataacac cactaccgtt tgtactatta cgattttgtt ttgctgcatt
     9121 gcttaatgaa tcttctcgat tattgaaaat accaggatta gacacagtaa taactttgcc
     9181 atttgtgtct tgaatgcttg ccagttcttc cttgcttaaa tcattcaatg accaaatttg
     9241 tctggtatca aaggatactt tacctgtttt ttcatctatt atcttcttat ggaaccacat
     9301 ttctttaggt gcagttgcag accttaccgc tctatcggct tgggttgtgg caaacatact
     9361 ccaagccata tcagaactcc ctgctcccca aacgagtcct aatcccgcat caagccaacg
     9421 aattttttca tctaattcat tagcttcctt atttaatgct ctagatttat cattgtcata
     9481 tccaccattt ttgagtctct cttttgtggc ttgctctcgt ttgctatcta ctactgcata
     9541 aagttcttgt ttcactcctt gtctagtttt atcaaactct tgggttactt ccctttgtaa
     9601 atccagttca ctttgcaccg cttctttgtt gaaggtgttc ttcaagctgc ccgaatgtcg
     9661 ttcggcggtg tctgtggtta cgtttgtatc aatatcggct ttggtttgtg ccgctgtttt
     9721 gcctgtcagc ctgatttgtc ccgcttcgtc ggtgatgtgt atgttgcggg tgttgatgcc
     9781 gcttttcgtg atgctgcttt gactgtcgct gtcgctgcca taacccactg atgaacttgc
     9841 gccgttttta tcggctacgc ttgtcaggtg tttgttttga ggtttatttt gtgcgccctg
     9901 tcccagtgtt ttgccgctta cggcaacgct ggcacccaat ccaaaacttt ttccttggta
     9961 ttggctgtaa ttttgaatgt cgctggtagt aagggtggcc gtctgaaagc ggtttttacc
    10021 cttgtcttct gcgctttggc tgctggtgat gatgccgcct ttgaggtctg tatggtttcc
    10081 gaccttgatt tgatagccgt cttctccggc ataaataccg ctttgctcgg ttactgaagc
    10141 atggtcggct cggattttgc tttgactgta atcgccactg gcactgaagc cataacctac
    10201 ggtcacttgt gcactggcgt tttgttgttt gctttgataa gtttctctat cttgtacgct
    10261 ttgaatactt aggtttttgg cattgacttg tacgcctttg ccgcgtactt gcgcgccttt
    10321 gatggtagtg tcgccaccgc tttggataag ggtttggctg cctttgtcgc caatatggct
    10381 atggcggtgg gttacgctat cgccattgcc gtagcctttg ccgacattgc cgcctgcggt
    10441 aacgcctaat gaccagcctc cttgtccgaa tgatacggca gcacctgcgt tccaacctgt
    10501 cgatttgttt tggctgcgtt cggtattgct ttgctcggct gattggagtg tgatgtcgtt
    10561 atcggcaatc aggattgtgc ctgctttgcc ggcaatatct gagcctgcga tgttgatatt
    10621 ggattgttct gctgcgcctg tggcgattaa tgtggtttta ccgcctgctt gaatttgact
    10681 cgcttgggct tggcttgctt gaacttgggt ggtttgtcgg ttttgctgtt cgccgtaggt
    10741 tatggagatg ctgacttgtt tggcattggt tgtaccattg gctaagtttt gtgcactctt
    10801 acctgtttga taggcttgcc agcctgcatt ggcagctgcc atggcattga cacgaccatt
    10861 tttgctttgt ccgacttgtt tgctgctttg tgcgacggca atcgcttgtt gtgccaaatc
    10921 ggtaacgggc gaactgaatg ccaccgttaa gcctttttgt tcataggttt gggtggtatt
    10981 actgtttaat ttgttgtgtg ccgcttgaat gtctatgctt tgggcatgga tgatattgtt
    11041 gccttctggg ctggaaacgg tactgccgat ttgttcgtag tgtttgcctg caacaatggt
    11101 ggtatcgcct ttcaagctgc ctacggtact gcctgtatgt tcgttgcttt gggattggtt
    11161 ttcttgtgtg tttgtcttgc tgccaatagt gaagccgata cctgcactca tcaatcctga
    11221 tttctgggtt tgatgatagg tttcgctttg gctttgagtt tgggttgtac caatgcgaac
    11281 atgattgcct gcttgaatcc gggtgccatt atcggaaata acattgctgc caaggatgtt
    11341 ggcatcgttt cctgcctgca atacaacttg cctgccgtca aaggtgctgc tttgagcggt
    11401 ttcgttgtgg ctctgggctt tgtcggtaat aatcaattta ttgccaccac cgcttctgcc
    11461 tgtgtgtttg gacgcatcat caacatgggt cgtgttgatg cctgcgctga tgttgatgtc
    11521 atttttggca gacacagcga gtgtaccgtt tgcgctgctg acttcggcag ctttggcatt
    11581 gaggttattc cctgacaata gggtaacatc gccttttgtt tgaatgctgc tgccgacttc
    11641 gttcgttgaa ccgcgaataa catggttatc ggcatcaaaa tgggttgctt gatgtttgcc
    11701 ggtttgtacc gtatctaggt taatgtcgcg ccctgcttgc agccgggttt gcccttgctc
    11761 tgattgattg ctgatttgac cggcaatgat gttgatgtct ttgcctgcct gcgctgctaa
    11821 aacacctttt tctttgcctg tgatataaat acctgccatt cggtctaggt aggtgctgct
    11881 gccttgtgta ttttgactgc tggcggcggt gctttggctg ttgatgttgt tgcctgcgtt
    11941 gagcaataat gtctgttcga cagaaagaat gccgccaata ttattgatgt cttgtgtggc
    12001 cgtaaccgct gatttttgcg catgaatacg cccaccgata ttgtctagcg tatcggtatt
    12061 gataataagc gcattgcgcc ctgcaatcgt gcctgagttt ttcaggctgc ctgaaacatt
    12121 gatttgtgta ttgctgcctg acaacaatgc acctttaccg tctatgtcgc cattcttaac
    12181 gcgtacataa acctgtggca ccaatacggt ttgtgtgccg ccatcaggaa gtttaacttc
    12241 tttttgtacc aaccaaacaa tatcgctggt cagttgcgct acttgctcgg cacttaatgc
    12301 aatgccaacg ctgagattca tggagcgtgc cgcagtcgcg ccattatcca ttaaggcttt
    12361 aaattgttct tcgtcgtttt gataaccgtc taaacgacga tgccctgtca gctctgcgat
    12421 ctgttcattg attaaacgtt gctcgtaata accatcaccc aaacgtttat gtaaattgtt
    12481 tgggtctagt ttgaggctgt ccagcatata gtcactaccc aaccattgac ggtagttggc
    12541 aaagcgtgga tcggtttcaa caagatagcc tttattgaca ggattgataa tatataagct
    12601 gctgctgggt aatggggtaa aagaattgga cgtatagggt agcgaaatac cgttgctttg
    12661 cggcaactca gtgccttggc tgggcgcatg atggcttaat gctttgcgat gcgattcata
    12721 ggcaaatgaa cccagtgaaa tgttgcgtgt gatttcctcc ggcaaagtgt aattttgttc
    12781 gctatgtccc gttgagtctc gtcctttatg tttctcacgc caatagctgt gtaatttgcc
    12841 attttcactg aatactttct tttcgccaaa ggtttgctcg ttatgcaaac cgtctttttc
    12901 tgtttgcaca agcaaattgc cgccggcgat gatttggcta tcggtattaa atacttcttt
    12961 accatcaatg gttaaatcat tacctgaaat gattttggct ggcgcagttt gggtaacttg
    13021 ggttttttgg gtgacttttt cataatcgta tttatgccaa ttttcatgcg ccgctccatc
    13081 aggggtgcgt aagtggtctg attcatcgtt atagacagac cagcctaatt catgttgtgt
    13141 gccttcccgc aataattcgt gtcgtccaaa tgcttcgtaa tcaacaatat gctcgcgccc
    13201 tgtttctacc aactgcgttt tcaaatgctc attggtattg tgcagctttt ctacgcctaa
    13261 acgcattttg ccggcggctt caatggttgc gccggcattg tgtatccttt gggctttgcc
    13321 tgtggcttgg ccattggtat ctaatgcgcc gccaaccgcc atatcgttac cgctgtaaat
    13381 cagactgttt tcacggttgt ttaattgtcc gatgcctaaa ttcaggtttt cacgtgccgc
    13441 aatggcggca cctgtaccgt tttcatcttg attgtctaag cgggtagccg caatagcgat
    13501 attgtcgcca taaatccgac ctgtaccgat attattcatt tgcccggctt ggattttggt
    13561 ttgttgtccg tcaatcaagc ctctattggt taaattgtgc tgcgtgccaa tgtctgttgc
    13621 accgccggat tgaatgttgc cttgtgctgc attatcaagg ttatttgctt taatccgaat
    13681 gcgttttcct gcttgcaaag tatgtgaatt tttcaggctg cctcgtgtac tgagcgacaa
    13741 ttcattgccc gccacgatat tgcgttctac ataaaaatca tcttgtaacg caatatccag
    13801 tttattatca gcggcaagtg tgccgttgtt ggataacgat tttgcctgaa tagcaacatc
    13861 acggcctgat tgtatcgtgc cattcgtatt atcaatgaca gcggtagatt gctgaccatc
    13921 gtgaataatc agttgttgat tggtcgctat ttcgccattt tgattgttca ggctgcctga
    13981 aacggctaaa tccgctattt ctgctgataa taacttgcca tgagcgttat ccagttgatc
    14041 ggtttcaatc tctaactgtt ggcgtgttgt gatgttgcca ttttgattat tcaggctgcc
    14101 ggcttgaatg tggaccgcat cactgataat tgttccattg tgattgtcaa acgccgaacc
    14161 ttttgcattt aactgatgaa tgtctatttg tcctgcatta tttaaacctt gttgcgcact
    14221 aacatctgtt tgaccattgg caataatact gcctgaatta tccagtgcac catgagtgcg
    14281 aattgtccca tcagcaaagg taggcgcagt tatgtttgat atagaaacgg ttgcagtacc
    14341 cgtacctgtt gccgttgttg gtgtggtagt ggatgaatgg aaagatgcat tgtaactatt
    14401 gccggtttga ttgcttgaac catttgacgc ggttggtgcg gtatcttgta aacccatgcg
    14461 gccacggtta tccattttgc cttgtgcatc aatatggagt ttttgtgaac ctgtttgaga
    14521 gagtttgcct tgattattaa gtgtgtcggt atcaatagcc aaacgagcgg cttcaatggt
    14581 gcctgatgtt tcatttttca ggctgcccga attgtgaatc aatatttcgc ctgaggacaa
    14641 taatgtgcca gtgttttgaa tcgactgact gtgaatttga gtgccttgtt gcgataccgc
    14701 cgtaccgctg ttttcaacgc cctgactgcg gatattgact ttgtgttccg ctgtattatc
    14761 cgtatctttc gcattggcgg cagccatcgt gccactattg actaaacggc catttgcatc
    14821 aatcgccaca ttgccggctg aggcaaacca ttgcccctga ttgcgcacgg atgcatcacg
    14881 atcagtgctg atcagggtga tcttaccggc atacataccg cccaatgccc ctgtatcgat
    14941 ggcatacagt gagttgtcgg aatttatatt cgtagcaggg gagtgtgaag actggataga
    15001 gccatcagct gagacatcat ttttccctgc cgacacacgc acatctttac cccacacagg
    15061 cccatcaatc ttaaccgcat ggctcaatat ctgcgtgaaa tcggtatcac gtgcatccaa
    15121 accttgtccc gtgatcgcaa tatgacccga acgaacctga aagcctgtta atgcgcctgc
    15181 ttgatattgc ggttggcctg tcgtcaaagt agcacgggaa gcattgataa aaccaccacc
    15241 attgactgca atccctgccg gattggcaat aacgacttct gcacgtcgtc cgcccacttc
    15301 aatatagcca ttcatttgtg aagaatggct gctgttgatt tggtttacaa ccacacgtgc
    15361 ttcgcccctt gccaaccaaa gattgccttg aatccaaccg cctagctgtg tttgggtgtt
    15421 gctgcggctg ttgtttaaaa tcgccccgcg attacccaca tcaaactgtg tatattgatt
    15481 aacagaaacc cctgccgaag taggggtttg aatattgact tgcggtatgc cgttacctgt
    15541 ttgcagaatc gtggcttgtt gggttttagg agcagcttta tcagcaataa tgccatcagc
    15601 aaaagcaata ttggccgtac ctacagccaa acataaagaa aagcccaata aagaaaaaga
    15661 aaagatattt gaacgacaaa caggtgcatg agtagtacca aaaggaacag atttcacatg
    15721 agcgctgcct gaatcactat cggcacagct tttaccttcg cgcttggtag tttcagcaac
    15781 ggctaccaca gccccacgtt tgcggttgaa aattacacga tagagagttt tattcatgat
    15841 ttcagttatt tgatttttat agagttatta gaaaaaattg gatagtctga ccattctaga
    15901 tcaaggattt tggcgagtca attaccgcca ttttactgcc atttgtttat taaattaggg
    15961 actttactag ataacggtta aaaatcccat tcgaacgaaa tggcaaggtt tataccgtcg
    16021 ttgtccctaa tgcgcaatca gcaacattat tgccgattat ccgagagaaa gttaagtctg
    16081 atggcattgt gtatacggat acctttcgta gttatgatgt acttgatgtc agtgaattta
    16141 gccatttacg caagttttcc agtatttgac tggcaattta aaacagtcgg attttgtccc
    16201 atttgttggc caagtcttta cttgcttggc cgtttgaatt taaaaagcag tctttctact
    16261 ttccgacctt tttttctgtt gtaaggtcta taatccaata gcattcccaa agagcatttt
    16321 ggacg
//
LOCUS       DAXHPG010000032        15379 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXHPG010000032 DAXHPG010000000
VERSION     DAXHPG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15379
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.032"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="ACFM6F_000751"
     CDS             complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="ACFM6F_000751"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6164213.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAPAAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAK
                     SRKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASG
                     RAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIH
                     PHPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1909..2346)
                     /locus_tag="ACFM6F_000752"
     CDS             complement(1909..2346)
                     /locus_tag="ACFM6F_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164214.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2440..4038)
                     /gene="aceF"
                     /locus_tag="ACFM6F_000753"
     CDS             complement(2440..4038)
                     /gene="aceF"
                     /locus_tag="ACFM6F_000753"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC6164215.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGASAAEAAPAVAAEAQPAPAAAPV
                     AAGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGV
                     VKAVFLKVGDKVSEGSAIIEVETAGSVAAAPAQAAQAAAPAPTAAAAPAAAPAPSAPA
                     AAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGA
                     AKPAAAGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTV
                     HEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLML
                     KNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACF
                     TISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVI
                     DGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4188..6851)
                     /gene="aceE"
                     /locus_tag="ACFM6F_000754"
     CDS             complement(4188..6851)
                     /gene="aceE"
                     /locus_tag="ACFM6F_000754"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC6164216.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7545..9257
                     /locus_tag="ACFM6F_000755"
     CDS             7545..9257
                     /locus_tag="ACFM6F_000755"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC6164217.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERTAAQAELTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGG
                     SLGPVSFAGKVYADFATEKGADWVIGANEDGYHYTSFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     LLNG"
     gene            9320..9976
                     /locus_tag="ACFM6F_000756"
     CDS             9320..9976
                     /locus_tag="ACFM6F_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC6164218.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9986..10441)
                     /gene="ruvX"
                     /locus_tag="ACFM6F_000757"
     CDS             complement(9986..10441)
                     /gene="ruvX"
                     /locus_tag="ACFM6F_000757"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC6164219.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10434..10982)
                     /locus_tag="ACFM6F_000758"
     CDS             complement(10434..10982)
                     /locus_tag="ACFM6F_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC6164220.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10984..11544)
                     /locus_tag="ACFM6F_000759"
     CDS             complement(10984..11544)
                     /locus_tag="ACFM6F_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC6164221.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11872..13677
                     /locus_tag="ACFM6F_000760"
     CDS             11872..13677
                     /locus_tag="ACFM6F_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6164222.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSVRKGHEGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13797..15281
                     /locus_tag="ACFM6F_000761"
     CDS             13797..15281
                     /locus_tag="ACFM6F_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC6164223.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELLKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3684 a         3780 c         4255 g         3660 t
ORIGIN      
        1 ccggaaaaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacgacc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggaggctt
      421 tggcggacaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgcccg
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcagcg gcaacgtggc
      541 cttcgtgaac ggctttgtgc gccaacatcg gttgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac gttggtacgc atttgtttgt ccacttcgat gaagccgcga tcggttacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttcg gtgcatttgc accttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttca ccaagtcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aatgccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagtgg
     1141 ctgcctgttc gtacgcgtcg ccggcagtca gcgacacttc caagtggtgc gggtctaaga
     1201 attgcccgtc gccttggata acgtccactt tacggctttt cgccatacct gccaaaccgc
     1261 ccgtgaggcg ggaaactacg ccgtctttgt aggcgcgaag catatcgatg tcgagttccg
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacaa ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaagttt
     1441 tgtaacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg accgccaccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 caggtgcttc agctttggga gcggctgccg tgccttcagc ttcaacgacg acaatcaagc
     1681 cgccttcgga gattttgtcg ccgactttga ctttcacttc tttgaccacg cccgcaactt
     1741 cggcaggcac gtccatcgtc gctttgtcgg tttccagggt aatcagggta tcgtcaacgg
     1801 cgatggtgtc tcctacgttc acttcaactg cgataatatc tacattttcg tgtccgccaa
     1861 tgtcgggcac tttcaattca actaaagcca ttatattgac ctttcaactt agttaatttc
     1921 tatcaactct ttagcaataa tcacttcttc aggaatacag ccacaatctt cagctctgat
     1981 tgttacttcc ttctcatttg ggttttctgg atgttcaacc tcatattcaa aaatagaata
     2041 ttgaccaaac aaatccctat ttaaaacata tatatagcca ttttcgatgc cggaacttgt
     2101 tgcaaatttc ttggcaattt ccttgtctgt tgtcgtagat atataacatc cgtcatatag
     2161 acctgtttca atttgatggg cgtaaactgc attcttcaca cttggaccat gtgtagcttt
     2221 accatcatat ttaaacttac catcataacg aattgcaact tcagctttat tacctttagg
     2281 ttttaactgt ccattatttt gctcatcttg ttggcaacta atgcctctat ataagaaatt
     2341 tcccataagt tattaattta aaggttgaat ttaacaccat tactcatcaa tctcaagcaa
     2401 tccaaacatt atcagactgc ttgagaggga tgttttattt tataaggtaa tgcggcggaa
     2461 gtctttcaac agcttcgcca agaatacggt gaagcgcata ccggccgcac cgtcgatgac
     2521 gcggtggtcg aaggacaggc tcaacgggca catcaggcgc ggggcaaact ctttgccgtt
     2581 ccaaaccggt ttgatttggg atttgcacac gcccaagatg gcgacttcgg gagcgttcac
     2641 aatcggcgtg aagcctgtgc cgccgatgcc gcccaagctg gaaatggtaa agcatgcgcc
     2701 ttgcatttct tgtggtttga gcttgccttc gcgggctttt ttggacaatt ctgtcagctc
     2761 ttggctgatt tgtttcaagc ctttttgatc cacgtctttg atgacgggaa caaccaagcc
     2821 gttcggcgta tcggctgcga aaccgatgtt gaagtagttt ttcagcatca ggttgtcgcc
     2881 gtccagagaa gcgttgaatt cggggaaggc tttcagcgcg gaaacggagg ctttgatgat
     2941 gaacgccaga ggagacagtt tcacgccttc gcgttcccat tctttgttca gttgtttgcg
     3001 gaattcttcc agctcggtca tatctgcttc ttcgtgtacg gtaacgtggg gaatcaccac
     3061 ccagttgcga gacaggtttt gaccggagat tttcttaatg cgggacaatt ctttaacttc
     3121 gacattgccg aatttggaga agtccacttt aggccacggc agcaagtcca gaccgctgcc
     3181 caaagatgcg ccggctgcgg caggttttgc cgcgccaccc tgcattacgg atttcacaaa
     3241 ggctttgatg tcgtcgccca cgatacggcc tttcaagccg gtacctttga cttggcccaa
     3301 atccacgccc aattcgcgcg ccagtttgcg tgcggaaggg ccggcgtgtg ctttggcgaa
     3361 agcggcctcg tcgattttgg cagcggcagg tgcagaaggt gcaggcgcgg cggcgggtgc
     3421 ggcggcagca gtcggagccg gtgcggcagc ttgagcggct tgagcaggag ctgctgcgac
     3481 agagccggcg gtttccactt cgataatggc agagccttcg gacactttgt cgccgacttt
     3541 taagaatacg gctttaacga cgccggcagc ggtacaaggt acgtccattg tcgctttgtc
     3601 ggtttccaaa gtaatcagcg tgtcgtcttc ggcaacggtg tcgcccactt tgatttcaac
     3661 ggcgattaca tccacatcgg tatgaccgcc gatatcggga acggctactt gaacggttgc
     3721 accgcctgcg gcaacgggtg cggcggcagg tgcaggttgt gcttcagcgg caacagccgg
     3781 agcggcttcg gcggcggagg caccggtttc aacggtcaaa attacgccgc cttcggagat
     3841 tttgtcgccg actttgactt ttacttcttt cacgacaccg gcagcatcgg caggcacatc
     3901 catcgtggct ttgtcggttt ccagtgtaat cagggtgtcg tcaacggcga tggtgtcgcc
     3961 cgctttaact tctacggcga tgatgtcgac gttttcgtga ccgccgatat cggggacttt
     4021 gatttctacg atactcattt gagttccttt aatcttgttc agttggatgc cgtctgaaag
     4081 ctgcttcagg cggcatcggt acggtttgtc tgctcaaacc attcgggcat tcggttttca
     4141 gacggcctga tgtcggaatg gggcaaacag gccgtctgaa acatcaatca gcgtttccag
     4201 ctaggagctg aatcggcttt gatgccgtat ttctcaatgg cttgttgaac ggtttctttg
     4261 ctgactttgc cttgttccgc caatgcggcc aatgcggcca cggcaacgtt gtagcgatcc
     4321 acttcaaaga agcggcgcag gttggcgcgg ctgtcggagc ggccgaagcc gtcagtaccc
     4381 aagacgtggt agtcgttcgg gatgtaggcg cggatacggt cagcatagct gcggatatag
     4441 tcggtagcgg caataaccgg accgtcatga ccttgcaatt tggaagtaac gaaaggtact
     4501 ttttcggctt ccagcggatg caggcggttg aagcgttcta cctcgacagc gtcgcggtgc
     4561 agcaggttga aggatgggca agaccaaatg tctgcttcca cgccgaagtc ggctttcagc
     4621 agttcggcac cggcaatcac ttcttgcaaa atcgtaccgg aacccatcag ttggactttc
     4681 ttgtcgcctt tgccgccggc tttcagcagg tacatacctt tcaagatgtc ttgttccgca
     4741 ccttcgggca tatccggatg ggtgtagttc tcgttcatca gggtgatgta gtagaacacg
     4801 tcttcattat tggcatacat acggcgcaga ccgtcttgta cgatgacggc gacttcgtat
     4861 tggaaagtcg ggtcataaga tacgcagttc ggaatcaggt cggcctggat gtggctgtgg
     4921 ccgtcttcgt gttgcaggcc ttcgccgttc agcgtcgtac ggccggcagt accgcccagc
     4981 aggaagccgc gcgcgtgcat atcgcccgcc gcccaagcca agtcgccgat acgttggaaa
     5041 ccgaacatag aatagtaaat gtagaacgga atcatggcga agttgctgtt ggcgtagctg
     5101 gttgcagccg caatccagtc ggccatcgcg cccggttcgt taataccttc ttgcaagatt
     5161 tgaccgtcaa cggattcttt atagaacatc agttggtctt tgtcttgagg ggtatattgc
     5221 tgacctttcg gattccaaat accgtattgg cggaacatac cttccatgcc gaaagtacgg
     5281 ctttcgtcgg gaacgatagg tacgacgcgt ttgccgattt ttttgtcttt cagcaaagtg
     5341 gacaggatgc ggacgaatgc catcgtggtc gagaactcgc gttcaccgct ggatttgagt
     5401 tgtgcgtcga atgctgacag ctcgggcact tccaatactt cctgcgtcgg tttgcgttgc
     5461 ggcaggtagc cgcccaaagc atcgcggcgt gcgtgcaggt atttgtattc ttccgtatcg
     5521 ggggcaaaag tcaggtaagg cagatcgccg ctttcgattt gttcgtcggt aaccggaatg
     5581 tcaaagcggt cgcggaattg tttcagggac gctttgtcca tttttttggc ttggtgggca
     5641 acgttctgac cttcgccgga tgcgcccata ccgtaacctt taatggtttt cgccaagatg
     5701 acggtaggtt tgccgtcggc atggttcgct gcgcggtcgt aggcgttgta cactttttgc
     5761 gggtcgtggc cgccgcggtt caatgcccag agttgctcat cggtcatatc ggcaaccaat
     5821 gctttcagtt cgggcgtatt gaagaagtgt tcgcgcacat acgcgccgtc tttggatttg
     5881 taagtttggt agtcgccgtc caaacattct tccatacgtt ggcgcaggat accgtctttg
     5941 tctttcgcca agaggcggtc ccagcggcgg ccccaaatga ctttgacgac attccagccg
     6001 gcgccggcaa agttgccttc caattcttgg atgattttgc cgttgccgcg taccggaccg
     6061 tccaagcgtt gcagattgca gttgatgacg aaaatcaggt tgtccaagcc ttcgcgtgca
     6121 gccagtgcga ttgcaccttg agattcgggt tcgtccattt cgccgtcgcc gcagaaacac
     6181 catactttac ggcctttggt ttttgccaaa ccacgcgatt ccaagtattt caggaaacgc
     6241 gcctgataaa tcgccatgat gggccccaag cccatggata cggtcgggaa ctgccaaaag
     6301 tcgggcaaga ggtgggggtg cggataggaa ggcagaccgt gtccgtccac ttcttggcgg
     6361 aagttgttca gctggtcttc ggtcagacgg ccctcgacga atgcgcgtgc atagatgccc
     6421 ggggcgacgt gaccttggaa gaagaccaaa tcgccttctt cgccttcgcc tttggctttc
     6481 caaaagtggt tgaaaccgac ttcgtacatg gtggcggcag attggaaaga tgcgatgtgc
     6541 ccacccagtt ccaaatcttt cttgccggcg cgcaatacga tggcggcggc gttccagcgc
     6601 acgaatgcgc ggatgcggtg ttcgatgttt tggtcgcccg gaatgccttt ttcgttttca
     6661 accgaaacgg tattcaaata cggggtggtc gtgccgtgtg gcatacgtac gcccttgtcg
     6721 cggcagtatt tgaccaggtt ttccaagagg tattgcgcgc gttcgccgcc ttcatattcg
     6781 aggacggagc ttaacgcgtc cagccactct tgggtttcga tagggtcaac atcgtgtaat
     6841 tgggtggaca tagtatctat cctttatgtt gagtgtttaa tcggcaggga gggtgtagcc
     6901 aaccccttta ttttcgtttg tgaaaaataa taagcaaaaa cgaaaatttc taaatcaaac
     6961 cgaaaaaaat aatttaagtt taatgttttc aaaaaattat aaaagttttg gaaacaaggg
     7021 aaaacttgaa aaaaatgttc gatctgctcc acacgaaatc ctatcagcaa tcgggtctgc
     7081 tggcaaataa ccatgcctgt aaaagcaggt tttatactga agttggcgtt tggtaaggtc
     7141 aagtcttttt gatttcgaaa tattctgttt gattttggcg atattttttc ggaattgtgg
     7201 tatatcgaat cattatgtct ctgtcggcaa ttttcctatt gtagaaaatc tatacacaag
     7261 ctttgtggat aaccgtgttg ataagccacg gcttgtgtta aaaaagtctt acgcaacaaa
     7321 tgtacgctta ttttgattaa aaaataggca catttaaatc tataaaaatc atgtgcttat
     7381 gttcaatatg atgtttctgg tcatgttttt gccgtctgtt atgggatttg tcccgagctt
     7441 ggggcagggg gtgaattttg gaggttggtg caaaacctgc ttttttcgcg ttacaatctg
     7501 cacccgattt acttttgaat ttgcctgttt ggaaaaatac ttttatgaaa gccagccaat
     7561 tctttatctc tactttaaaa gaagcccctg ccgaagccgc gcttgccagc cacaagctga
     7621 tgattcgcgc cggtctgatt aaagccaacg cgtccggcct ttatacttgg atgccgatgg
     7681 ggctgcgcgt gttacgcaaa gtcgaaaacg tcgtgcgcga ggaaatggcg cgtgcgggca
     7741 gcgtggagtt gctgatgccg gtggtgcagc ctgccgagct gtggcaggaa tccggccgct
     7801 gggagtttta cggtaaagag ttgctgcgcc tgaaagaccg ccacgaccgc gatttctgca
     7861 tgggtccgac ctgtgaagaa gtcatcgccg acatcgtgcg caaagaaatc aacagctaca
     7921 aacagctgcc gaaaaacttt taccacatcc aaaccaaatt ccgcgacgaa gtgcgcccgc
     7981 gtttcggcgt gatgcgcgcg cgcgagttcg tcatgaaaga cgcttattcc ttccatgccg
     8041 actacgcctc gcttcagacg acctatgatg ccatgtatga cgcttactgc cgcatcttca
     8101 cccgtctggg cttggcattc cgccccgtcg ccgcagacac cggcagcatc ggcggtacgg
     8161 gttcgcacga gtttcaagtg ttggcggaaa gcggcgaaga tgtgattgcg tacagcgaca
     8221 cttccgatta cgccgccaat atcgagttgg caccgacctt gccgcttaaa ggcgaacgca
     8281 ccgctgctca ggccgagttg accaaagtac atacaccaaa cgtcaaaacc attgagtctt
     8341 tggttgaatt cctgaatatt ccggttgaac aaaccctcaa atccatcgtg gttgaaggcg
     8401 aaaacgaagg cgaaatcgtc ttattactgt tgcgcggcga ccatgagttc aacgacatca
     8461 aggcagaaaa actcgcaggc gtgagatcgc cattgacgat ggcaagtcct gccgcgattg
     8521 tcgaacagtt cggcgcaaac ggcggctcgc tgggtccggt cagctttgca ggtaaagttt
     8581 atgccgattt tgctactgaa aaaggagcgg actgggtcat cggtgcgaac gaagacggct
     8641 accactacac cagcttcaac ttcgggcgcg atgccgccga gcctgaattc gtcgatttgc
     8701 gtaatgtggt cgaaggcgac gaaagccccg acgggcaagg ccgtctgaaa ctcgcgcgcg
     8761 gcattgaagt cggacacgtc ttccagttgc gcgacaaata cacccaagcc atgaacgtaa
     8821 gcttccttga caacaacggc aaatcgcaaa ttatggaaat gggctgctac ggcatcggca
     8881 tcacccgcgt cgttgccgcc gccatcgagc agaataacga cgaaaaaggc atcatctgga
     8941 ccaaagcaat ggcgccgttt gaagtcgtta tcgtgccgat gaactacaaa aaatcagaca
     9001 ccgtacgcga agccgccgac aaaatctatg ccgaattgct ggcggcaggc gcggatgtgc
     9061 tgctggatga ccgcgacgaa cgcgcaggcg tattgctgaa cgattccgag ctgttgggta
     9121 ttccgcaccg catcgtcatc ggcgaccgcg ctttgaaaga aggcaatgtc gaatacgccg
     9181 aacgccgcaa caacgaagcg caggcagttg caatcggaga aattgttgcg cgtgtaacag
     9241 ctttattaaa tgggtaagct tggtcaaatg ccgtctgaaa ataatttcag acggcatttt
     9301 tatttgggga gaaagtgaag tggcttcagt atttttagaa ggagaagcag tcgaggtcgg
     9361 caatattttc tgtatcggca ggaactatgc cgcacatatt gaagagttaa aaaacgaaat
     9421 accttccgaa cctgtggtgt ttatgaagcc gtcaggcagc attttgaaca gcggcggtac
     9481 aatcttgctg cccgagttta gtagggatgt gcagtttgag tgcgaactcg tgctgctggt
     9541 cggtaaggat tcagacggca cgggcgaggg taaagatatt ttgggatgtg tcgcaggata
     9601 tggtgtaggg ctggacctta cggcacggga tatccaatgc cgtctgaagg aaaaagggct
     9661 gccctggctg aaggcaaaag ggttcaggca ttccgcgtgc gtgtcggatt ttgcggcggc
     9721 aggcagaatc ggcaatcccg agaatgtgtt gttttcatta aaacaaaacg gcgtattgaa
     9781 acagcgcggg gataccggtt tgatgattta cccgattcgg gaaattttac acaaactggc
     9841 ggcagattac ggtttgggga aaggggattt ggtgtttacc ggaacgccct ccggcgtggg
     9901 cgcaatcggt gcgggggaca atttggcatt ggagctggac ggcttggtgc gcgcctcttt
     9961 cacggtaggg tgttaaccgc gccgcttaac cctcacgccc gttgaaacat tccgccggac
    10021 cgccctcgaa aaaaccgtgc aagatggctt gcgccgccac ttggtcgagc accgatttgc
    10081 gttttttgcc gaagacctgt gcttccgaaa gcaggctttc ggcatagacg gacgacagcc
    10141 gttcgtcaac ccaatagacg gggagattga acctgccgtt cagcctgcgt ccgaacttgc
    10201 gcgacaggtg cgtcatttca tgtttcgtgc cgtcggtatg cacgggcagt ccgacgacaa
    10261 aataacgcgg ctgccattct tgaaccagct tggcgattgc cgcgaacttt tcatcgttgc
    10321 tgccgccggt aacggtgctc aaaggatggg atagccctaa ttccgcgtct ccttgtgcca
    10381 cgccgatacg cgcttcgccg aagtcgaatg ccagtgccgt tccttttgga attttatgca
    10441 tggccggctc ccgaaaacag ggcgagcggg tcgatgccga gtttggcgaa tgcggcggcg
    10501 taacggtgtt cgtaggggat gtcgaacagg atgtgttcgt cggcgggaac agtcagccac
    10561 gcattgtcgg caagttcgcg ttcgagctgg ccttttcccc agcttgaata gcctatgctg
    10621 atcaaggctt tgtcaaccgc accttcgcgt gaaatatttt caatcacgtc tcgggaagaa
    10681 gttagcgcga tattgtctga aacgccgata ctgctttgcc agttgccgat cggggtatgc
    10741 acgacataac ctcgctcgac ttgcaccggc ccgcccatca tcacgctgtc gtgctgcatc
    10801 cgcatgggga tgtttttgcc ggtggcggaa aaaatcatgt ccatcgtaat cggagagggt
    10861 ttgttgatgg cgatgccgag tgcgccgtct tcatcgtgtt tgcagatata gacgaccgat
    10921 tgtgaaaaaa acgcgtcttc catatcgggc atggcgacga gaaagtggtt ggagaggttc
    10981 atatcagcgt ttgtcgggat tttctatcgg gttggaaatc atgcaggcgc cggcaagcag
    11041 gttgccggaa gtatcggctt gcaccccatc ggtttgcggt atgccgccgc cgaaacagga
    11101 aaccgcgctt aaggaatttt tcggcgagcc ttggatgatt ttatcgctgt acaccaaata
    11161 ggcgaaggtt ttgcgtttgg ggtcgtaata acggacaatc tgccggctct tgaacgcgaa
    11221 gctcgcaccg tgtttgaaaa cttctttcgg tttgcgcacg gcggtttcgt caaaagaaat
    11281 cgaagatgcc gtctgaacgc acgaaaccga tgcgtcggac gcgtcctctt ccaaattgac
    11341 catttccttc aagccgcctt tttttgcata cgaaatataa caggcaaccc cttgaacgtc
    11401 gggatcgtcg aatccttcca cttcgatacg gtcgtttttg cccagtatgt tgaaaacggt
    11461 acttgcccgt ccgattttat cggtttcgcc gctgccgcag gcagtcagca ggacggcggc
    11521 agacagcagt agcagtctgt tcatcaaaat ttccttatcg gttgaatccc cgttttccgt
    11581 gaaggcggca gggcttgctt tgtttggagg ggcgcacgtt cccaaaatcg ggcaacatac
    11641 ggtatgcggc ttaatgtgct accctgtgtg tcggaacaca atatccgttg cggcattgaa
    11701 aatatgatgc acaatataag gctgtttgag ccgattgcaa gtatccgcgc aaagaaggcg
    11761 caagattggg caaacggcat tttcagacgg cataactgac agtataatcc aaacgtccgg
    11821 ctgctttcgt gcagccttca gtattatcca actttttccg aaggtcgaac catgcgctac
    11881 aaaccccttc tgcttgccct gatgctcgtt ttttccacgc ccgccgttgc cgcccacgac
    11941 gcggcacaca accgttccgc cgaagtgaaa aaacagacga agaacaaaaa agaacagccc
    12001 gaagcggcgg aaggcaaaaa agaaaaaggc aaaaatggcg cagtgaaaga taaaaaaaca
    12061 ggcggcaaag aggcggcaaa agagggcaaa gagtccaaaa aaaccgccaa aaaccgcaaa
    12121 gaagcagaga aggaggcgac atccaggcag tctgtgcgca aaggacacga aggggataag
    12181 aaatcgaagg cggaacacaa aaaggcacat ggcaagcccg tgtccggatc caaagaaaaa
    12241 aacgcaaaaa cacagcctga aaacaagcaa ggcaaaaaag aggcaaaagg acagggcaat
    12301 ccgcgcaagg gcggcaaggc ggaaaaagac actgtttctg caaataaaaa agtccgttcc
    12361 gacaagaacg gcaaagcagt gaaacaggac aaaaaataca gggaagagaa aaatgccaaa
    12421 accgattccg acgaattgaa agccgccgtt gccgctgcca ccaatgatgt cgaaaacaaa
    12481 aaagccctgc tcaaacaaag cgaaggaatg ctgcttcatg tcagcaattc cctcaaacag
    12541 cttcaggaag agcgtatccg ccaagaacgt atccgccaag agcgtatccg tcaggcgcgc
    12601 ggcaaccttg cttccgtcaa ccgcaaacag cgcgaggctt gggacaagtt ccaaaaactc
    12661 aataccgagc tgaaccgttt gaaaacggaa gtcgccgcta cgaaagcgca gatttcccgt
    12721 ttcgtatcgg ggaactataa aaacagccag ccgaatgcgg ttgccctgtt cctgaaaaac
    12781 gccgaaccgg gtcagaaaaa ccgctttttg cgttatacgc gttatgtaaa cgcctccaat
    12841 cgggaagttg tcaaggattt ggaaaaacag cagaaggctt tggcggtaca agagcagaaa
    12901 atcaacaatg agcttgcccg tttgaagaaa attcaggcaa acgtgcaatc cctgctgaaa
    12961 aaacagggtg taaccgatgc ggcggaacag acggaaagcc gcagacagaa tgccaaaatc
    13021 gccaaagatg cccgaaaact gctggaacag aaagggaacg agcagcagct gaacaagctc
    13081 ttgagcaatt tggagaagaa aaaggccgaa caccgcattc aggatgcgga agcaaaaaga
    13141 aaattggctg aagccagact ggcggcagcc gaaaaagcca gaaaagaagc ggcgcagcag
    13201 aaggctgaag cacgacgtgc ggaaatgtcc aacctgaccg ccgaagacag gaacatccaa
    13261 gcgccttcgg ttatgggtat cggcagtgcc gacggtttca gccgcatgca gggacgtttg
    13321 aaaaaaccgg ttgacggtgt gccgaccgga cttttcgggc agaaccggag cggcggcgat
    13381 gtttggaaag gcgtgttcta ttccactgca ccggcaacgg ttgaaagcat tgcgccggga
    13441 acggtaagct atgcggacga gttggacggc tacggcaaag tggtcgtggt cgatcacggc
    13501 gagaactaca tcagcatcta tgccggtttg agcgaaattt ccgtcggcaa gggttatatg
    13561 gtcgcggcag gaagcaaaat cggctcgagc gggtcgctgc cggacgggga agaggggctt
    13621 tacctgcaaa tacgttatca aggtcaggta ttgaaccctt cgagctggat acgttgatcg
    13681 gattgccgaa acggtaggct ttatgcaaat cgtgccgata tggtgcgctg ttccgtgaag
    13741 tttgccgtac ctgtattttc cggtttttgg aaatcaagag agaaagttaa agaagaatgt
    13801 cgaaacctgt ttttaagaaa atcgcacttt atactttggg tgcaatcagc ggcgtggccg
    13861 tcagtctggc ggtgcagggt tttgccgccg agaaggacag gcgggataac gaagtcctgc
    13921 cggtgcaatc catccgcaca atggcggagg tttacggtca aatcaaggca aactactatc
    13981 aggacaaacc cgatgccgat ttgtttgaag gtgcgatgaa gggtatggtg gccggtttgg
    14041 atccgcattc cgaatatatg gataaaaaag gttatgccga gataaaggag tccaccagcg
    14101 gcgaatttgg cggcttgggg atggaaatcg ggcaggaaga cggatttgtc aaagtggttt
    14161 cgccgattga ggacacgcct gcggaacggg cgggggtgaa aagcggcgat ttcattgtga
    14221 aaatcgataa tgtttcgaca cgcggcatga cggtcagcga agcggtgaag aaaatgcggg
    14281 gcaagccggg tacgaagatt actttgacgc tgtcgcgcaa aaatgccgac aagccgatag
    14341 tcgtcaacct gacccgtgcc attattaaag tgaaaagcgt ccgccatcac ctgatcgaac
    14401 ccgattacgg ctatatccgc gtgtcgcagt tccaagagcg gacggtcgaa agcgtcaata
    14461 ccgccgcaaa agagctgcta aaggaaaata aaggaaaacc gctcaagggg ctggtgttgg
    14521 atttgcgcga cgaccccggc gggcttttga ctggcgcggt cggcgtgtcg gcggcatttc
    14581 tgccgtctga agcagtcgtc gtcagcacca agggacgcga cggcaaagac cgcatggtac
    14641 tgaaagccat tcctgaagat tatgtgtacg ggatgggcgg cgattcgttg gcgggcattc
    14701 ctgccgagtt gaaaaccata ccgatgacgg tattggtcaa ttccggttcg gcttccgcgt
    14761 cggagattgt cgcaggtgca ttgcaggatc ataaacgcgc ggtcatcgtc ggtacgcaga
    14821 gcttcggcaa aggttcggtt cagactttga ttcctttgtc caacggcagc gcggtcaagc
    14881 tgacaacggc actgtattat acgccgaacg accgttctat tcaggcgcag gggattgttc
    14941 ccgatgtcga agtaaaagat aaggaacgca tttttgaaag ccgcgaggcg gatttggtcg
    15001 gacacatcgg caatcctttg ggcggcgagg atgtgaacag tgaaaccctt gccgtgccgc
    15061 ttgaaaaaga tgcggataag cccgctgtaa aagaaaaagg taaaaagaaa aaggacgagg
    15121 atttgtcttc aaggcggatt cccaaccctg ccaaagacga ccagttgcgg aaagctttgg
    15181 atttagtcaa gtcgcccgag cagtggcaga agtctttggg gctggcggcg aaaaagccgg
    15241 tttcaaataa agataagaaa gataaaaaag ataagaagta ggttttgacg atgccgtctg
    15301 aaaggatttc agacggcata ttatgtttga aaggacaggt tatgagcgat tttttaccgg
    15361 aatttgaacc gtgcggcat
//
LOCUS       DAXHPG010000033        14925 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXHPG010000033 DAXHPG010000000
VERSION     DAXHPG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.033"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(97..3582)
                     /gene="smc"
                     /locus_tag="ACFM6F_000762"
     CDS             complement(97..3582)
                     /gene="smc"
                     /locus_tag="ACFM6F_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC6164224.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNRQQDLLDYAQWQQSLAAADKATEQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATGHAAAPQLWQHITAPAEWQHALSVILAERLHARDVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLSPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHASDGLSDDIATLQEAAAELEHQQQTAAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIARLEQQTLDWQERQQELVLAYETEFQNDEQHIKLDELT
                     ESVHTLDEEYIAVQEKLTQIQEQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQTAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAESV"
     gene            3831..4553
                     /gene="lpxH"
                     /locus_tag="ACFM6F_000763"
     CDS             3831..4553
                     /gene="lpxH"
                     /locus_tag="ACFM6F_000763"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC6164225.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAIYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            4822..6408
                     /locus_tag="ACFM6F_000764"
     CDS             4822..6408
                     /locus_tag="ACFM6F_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC6164226.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6576..6779)
                     /locus_tag="ACFM6F_000765"
     CDS             complement(6576..6779)
                     /locus_tag="ACFM6F_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC6164227.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6895..7392
                     /locus_tag="ACFM6F_000766"
     CDS             6895..7392
                     /locus_tag="ACFM6F_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC6164228.1"
                     /translation="MTALTLPEDILQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADEGRDIPSLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIRSNWETFA
                     HIGQA"
     gene            7474..8667
                     /locus_tag="ACFM6F_000767"
     CDS             7474..8667
                     /locus_tag="ACFM6F_000767"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC6164229.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIATEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQNFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9070..10005)
                     /gene="hemC"
                     /locus_tag="ACFM6F_000768"
     CDS             complement(9070..10005)
                     /gene="hemC"
                     /locus_tag="ACFM6F_000768"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC6164230.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGVVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVILQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10124..10639
                     /gene="ybeY"
                     /locus_tag="ACFM6F_000769"
     CDS             10124..10639
                     /gene="ybeY"
                     /locus_tag="ACFM6F_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC6164231.1"
                     /translation="MKRAKQYPFLSLQRQRFHLNFENASSTANLPSERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            10641..11465
                     /locus_tag="ACFM6F_000770"
     CDS             10641..11465
                     /locus_tag="ACFM6F_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC6164232.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11608..12987)
                     /gene="nhaC"
                     /locus_tag="ACFM6F_000771"
     CDS             complement(11608..12987)
                     /gene="nhaC"
                     /locus_tag="ACFM6F_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC6164233.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNGVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13279..14502)
                     /locus_tag="ACFM6F_000772"
     CDS             complement(13279..14502)
                     /locus_tag="ACFM6F_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC6164234.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3525 a         4189 c         3844 g         3367 t
ORIGIN      
        1 gctgtaccga tttaaattta attcactata ccgtctgaca acccgtatcg caacacttca
       61 ttaaaaacgg caagattcag ccgttctgca acccgttcaa acggattccg ccatttccaa
      121 cgcctgtttg atgtccacgg cgacgacgcg cgatacgcct ttttcctgca tggttacacc
      181 gaccagctgt tccgccattt ccatggtcag gcggttgtgg gagatgtaga gaaactgggt
      241 ttgcgccgac atttctttga ccaggttgca gaaacgcgaa gtgttggcat cgtctagcgg
      301 agcgtcgact tcgtccaaaa gacagaacgg agcggggttg aggctgaaca gggcgaacac
      361 gaggctcatc gcggtaaggg ctttttcgcc gccggaaagg aggtggatgg tgctgttttt
      421 cttgccgggc gggcgcgcca tgatagacac gccggcggtc aaaaggtcgt cgcctatcat
      481 tttgagggtg gcttcgccgc cgccgaacag ggtcgggaag aaggtttgga ctttgccgtt
      541 gacggcgtcg aaggtttctt tgaaacgcgc tttggttttg tcgtcgattt gggcgatggc
      601 ttcttccaaa agggtgatgg cggtctgcac gtcttcgctt tggctgcggt agtagccgtc
      661 gcgttcgcgc gcttcttcga gttcttgcag ggcggcgagg ttgaccgcgc cgagggcttc
      721 gatttgttgc gagaggctgc cgatgctgct gttcaatact ttcggcgatt ctttcgccaa
      781 cgcttcgagc gcatccaaat cggcggcgcg ttcggtcagg ttttgatggt agcgtttggc
      841 gttgatcagg gcttcctgtt gttgcaacag ggcggtttgg gtggcggcct gaagctgtgg
      901 caactgggtt tgcagggttt gtacgcgggc gtattgttcc ctaccctgct cctgaatctg
      961 cgtgagtttt tcctgcacgg cgatgtattc ttcgtccagc gtgtgtacgg attcggtcag
     1021 ctcgtcgagc ttgatgtgct gctcgtcgtt ttggaactcg gtttcatagg cgaggacaag
     1081 ctcttgctgg cgttcctgcc agtctagggt ttgctgttcg agtcgggcga tttgctgctg
     1141 gtagttttgt ttttgctggt tgagtttgtg gacggcgact tcggcaagcc catattggcg
     1201 gttggcttcc agcagggcaa gctgcgcctg tttcagacgg ccttgctgct cttggcggct
     1261 gtgtgcggcg gtttgctgct ggtgttcgag ttcggcggcg gcttcctgca aagtagcaat
     1321 gtcgtctgaa agcccgtcgg acgcgtgttg caacacggtc tgctcttccg ccaactgcgc
     1381 cagttcgcgc tcgatgtgtt cgcggcggat ttgcccttgg ttggtacgcg ccagaagttc
     1441 ggcggcgcgt tgctgcgctt ggctgtattg gcgcgtgtgc tgctgttgct gctgcatcag
     1501 gtttttatgc tgcacttcgg acgagcgcac ggcggcttcc gcctgtttga acgcggcttc
     1561 ggcggcagaa agttcggggg agaggttttc cagttcggac gcgatgccgt cgaggcgcgc
     1621 tttttgggca atcaggcttt cctgcgcggg tttggcatag agcaggacgc tgaccttatc
     1681 gacctgatgg ccttcgggcg tgagccagat ttgatgtgcg cccaaatcgt tttgatgcgc
     1741 gagggcgtaa ctcaaatcag gcgcacacaa tacgccgtcg agccagtggt gcaatgccgt
     1801 ctgaaacggc ggctgcgctt ggatttggtt cagcaatgcc tgtacgggca gggatttttt
     1861 gatgccgccg gagagaccgt ctgaaagcca cgccgcctgc ccctgcggca aaggttcggg
     1921 cggtacgaaa ccttgcggca cgtcgcgggc gtgcaggcgt tcggcaagga tgacggacag
     1981 ggcgtgctgc cactcagcag gcgcggtaat gtgttgccac agttgcggcg cggcggcgtg
     2041 gccggttgcc tgccagaaat cggctgcttc ctgctgttgc gacaggattt gcgacaacgc
     2101 ctgctgctgc gcctgcaagg tgatgtgttg ctgcttcagg ctttggaagc ggtttgaggc
     2161 cgtctgaaac gcctcgcggg cggcgtgtaa ggcttcttcg gcggcgatga tttgttcttc
     2221 gtaatgctct tgccggcttt gcaacaaggc agcggcctcc tgcgcggcgg cggtttccgc
     2281 ttcgtcgggc aggttcaggg cttggttctc ctgtttcaga cggcctttgc gctcttcgtg
     2341 cttggcaacg gtttgttcgg catgggcaag ctgctgctgc ttcaacgcca gttcgcggcg
     2401 gatgcggttt gcctcgtcct gctgggtttg gaaggcggca ttgagcgcgg tttgggtttc
     2461 ttccaattcg ggcagacgct cttcgtgttc ggcaacctgc atcgcccact ccgccagctc
     2521 ggtttgcttt tcttcgacgt ataactcgtt ttcttcaagc tgcacgcgga tttgctgctg
     2581 ctcttgatga atacgctgca actgcgcctg cgccgcctgc ttgtcgcgtt cgatgcgttg
     2641 gtgcaggttt tgctgatggc ggatttgttc ttccaaacgg gcaatctgct cgcgcaacac
     2701 gccgcgtttg ttgctcaatt catgcaccgc ctgttgctgc gactgttcgg cggtctgcaa
     2761 ggcgtgtact tcgtcgttta acgcctgaac ctgcgcggcg gtttcgtcct gctgcgcctg
     2821 caaagactga tgctgctcgg tcgccttgtc ggcggcggca agcgattgct gccattgggc
     2881 gtaatcgagc aaatcctgtt gccggttcaa ctgcgcggtc agggatttgt agcgttcggc
     2941 ggtttccgcc tgtttctcca gcttttccac ctgacgcgcc aattcgttct gcaaatcgcc
     3001 caaacgctgc aaatgctcgc gcgtgtcttt cagacggcct tccgtctcct tgcggcgttc
     3061 cttatatttg gacacacctg ccgcctcctc gatataggcg cgcaattcct ccggccgcgc
     3121 ttcgatgatg cgcgaaatca tcccctgctc gataacggca taaccgcgcg cgcccacgcc
     3181 cgtacccaaa aataaatcgg taatgtcgcg gcggcgcacg gtctgattat tgatgaaata
     3241 agtcgattcg ccctgacgcg tcagctgccg cttgatactc acctcggcat actgccccca
     3301 cgccccctgc aaactgtggt cgctgttgtc aaacaccagc tccaccgaag ccctcggcgc
     3361 aggacggcgc gtcgccgcac cgttaaaaat cacgtcctgc atactctcgc cacgaagctg
     3421 cttcgccgaa gcctcgccca acacccagcg caccgcgtca atcacattcg acttgccgca
     3481 gccgttcggc ccgataaccg caacaagctg ccccggaaca tgaatcgtgg tcgggtcggt
     3541 aaaagatttg aagccggaga gtttgatgtg ggtcaggcgc ataatggtcg gaaaaaataa
     3601 aaaagaagcg tattttaacg gaaatcccgc cgcaccaccc catatcttgc cggcaaaacc
     3661 ttaccgcatc ccgcacccgc gatgccgtct gaagccttca gacggcattt ttaacgcgcc
     3721 cgaaccccgg ttccgccaat gcccgatgct tggcgcggca tttgccgtat tgccccgccg
     3781 ccgggcgcgt tataatccgc cccgacatcc gcccgattgg aaacctgcct atgaaacccg
     3841 cctatttcat ctccgacctg catttgagcg aaaagcaccc cgaactgacc gcgctgctgc
     3901 tgcgtttttt acgttcttcc gccgccgggc aggcgcgggc gatttatatt ttgggcgatt
     3961 tgtttgattt ttgggtgggc gatgacgaag tttccgagtt gaatacttcg gttgcgcggg
     4021 aaatcaggaa attgtccgac aaaggcgttg ccgtgttctt cgtcaggggc aaccgcgact
     4081 tcctgatcgg tcaggatttt tgccggcagg cgggtatgac gctgctgccg gattactcgg
     4141 ttttggactt gttcggctgc aaaaccctca tctgccacgg cgacactctg tgtacggatg
     4201 acagggcata ccaacgtttc cgcaaaatcg tgcatcggaa gcggctgcaa aaactgttcc
     4261 tgatgctgcc cctgaagtgg cgcacgcgcc ttgccgccaa aatcaggcgc gtcagcaaaa
     4321 tggaaaaaca ggtcaagccc gccgatatta tggatgtcaa tgccgccttt accgcgcggc
     4381 aggttcgcgc ctttaacgcg gaaaggctga tacacggaca cacccaccgc gagcatatcc
     4441 atcacgaaaa cggctttacc cgcatcgttt tgggcgactg gcataacgac tatgcttcaa
     4501 tcctccgcgt ggacggggac ggcgcggtat tcgtgccgct ggaaaaatgc tgaaaatgcc
     4561 gtctgaagcc tttcgggcgg ctttttttac acgccccttt cccacactcc ctccgccgca
     4621 tcatcaccat ccttaacccg cattaatggg cgttatcgat aaaatgcttg accgattaca
     4681 taagattacg taaagtgtgc aaacgcataa caattggtct taccaattta tcttttaacc
     4741 aaaatatcgg gtgtacggga tttgaacgtc tttccaaacc tcgacgcgcc ttatgatgca
     4801 aatgcctaaa aaggattcgt tatggcactt ttcctcagca tattccccat cgtcctgctg
     4861 atttggctga tggtgaaaaa aaacagtatg ccctcctacg tcgcgctgcc gattaccgca
     4921 gtgctgattt acgccatcaa acttttctac ttcggcgatg cgggcatgct gctcaacgcc
     4981 accgccgctt ccggcctcgt caaaacgctc acgccgatta ccgtgatttt cggcgcgatt
     5041 atgttcaacc gtatgatgga aaccacgggc tgcattgatg tcatccgcaa atggctggcg
     5101 accatcagcc ccaaccccgt agcgcaactg atgattatcg gctgggcttt tgcctttatg
     5161 attgaaggcg catccggctt cggcacgcct gccgcgattg ccgcgccgat tctgatgagc
     5221 ttgggcttca acccgttgaa agtggcgatt ttcactttgg tgatgaactc cgtccccgtc
     5281 tctttcggcg cggtcggtac gccgacctgg ttcggtttcg caccgctgaa cctgagtgcc
     5341 gaagacatcc tcgccatcgg caggcagacc ggcgtaatgc acttcttcgc aggtttcgtc
     5401 atccccgtca tcggcttggg cttcatcgta ccttggtctg aaatccgcaa aaacttgggc
     5461 ttcgtcgcca ttgccgtctt ctcctgcacc attccttatg tcgcattggc gatggtcaac
     5521 gaagaattcc cgtcgctcgt cgccggcgcg atcggcctga tggtgtccgt attcgccgcc
     5581 aaccaaggct ggggcttgag caaggaccac gccaaagacc cgaatgccga aaaagtgccg
     5641 ttcgcccaag tcgccaaagc actcgcccct ttgggtatgc tgatcggcat gctggtggtt
     5701 acgcgcatca aacagctcgg tatcaaaggc attttgacca gcaaagaaga atggttcagc
     5761 ttccaactgc cgtttgattt gtccaaaatc accgtcagcg actccctgac gattaccttc
     5821 ggcaatattt tcggacaaga tgtcagcgcg tcttaccaaa cgctgtacgt cccggcttgg
     5881 attccgtttg tgctgaccgt ttggatttgc atcctgctgt ataaaaccaa attcaaagat
     5941 gcctggacga tttatgccgt aaccttcaat caaaccaaaa aaccgctgct tgccctgatg
     6001 ggcgcgctga ttatggttca gctgatgctg gtcggcggcg acaattcgat ggtgaaaatc
     6061 atcggtaagg aatttgccgc aatggcgggc gaacactggg tttatttctc gccgtatctg
     6121 ggcgcgatcg gtgcgttctt ctccggctcc aacaccgtgt ccaacctgac tttcggcccg
     6181 attcagcagc aaatcgcctt ggataccggc ctgtccgtta ccctgattct ggcgttgcag
     6241 tccgtcggcg gcgcgatggg caatatggtg tgcctcaaca acatcatcgc cgtatgtacc
     6301 gtattggatg tgaaaaattc cgaaggtgcg attatcaaga aaaccgttat cccgatggcg
     6361 atttacggcg tgattgccgt cgtcgcggca atgattttct tcctctaggc gaaacgccac
     6421 catccatagc ggcagcacgc ggggaacgga aaccgccgtt ccccgtctgg gcggcgcgtc
     6481 gggcagaatc ccggtgccgg aacagcccgc cccgccaaac aaatgccgtc tgaaaccgga
     6541 aaaggcttca gacggcattt ttccgccgtt tgcattcagg cgtaatgatg atgcggcatc
     6601 ttgccgtcaa ggcggtagcg cgtgccgcaa tacgggcagg caacgctgcc cgattctcct
     6661 tcgcacaaag gcaaaaacac tctcggatgc ccgttccact gctcgttgtc gggtcccgag
     6721 caatacagcg gcagattttc cggcaacacg gaaatttcct gcagattcag attgtccatt
     6781 tgattttcct ttgcgtggtt ggcgtgcgcc cattttacgc catcggcagg ctaaaggata
     6841 ttttcggcgc aaagccgcaa tccgctataa tccccacttt tcagacggca taccatgact
     6901 gcgcttaccc ttccggaaga catcctccaa caagaaccat ccgttctgct ctataccctc
     6961 gtttccgcct acctcgaaca caccgcccaa accggcgacg aatccctctc ctgcctgtcc
     7021 gacgaccagc acacgctgac cgcattctgc tacctcgaca gccaagtcga agaaggcggc
     7081 ttcgtgcaac tcatcgcctc cggttacggc gaatatattt tccgcaaccc gcttgccgac
     7141 agcctgcgcc gctggaaaat caaagccgtg ccgaaagtct tggacaaagc caaagccctc
     7201 tacgaacaac acggcgaaac catcgaaacg cttgccgacg aagggcggga catcccttcc
     7261 ctgcgcaaac agttccccga ctttgaagaa tgggacggcg catactacga agccgccgaa
     7321 caagacctgc ccctgcttgc agaacacata cggtcaaact gggaaacctt cgcccatatc
     7381 gggcaggcgt gattgcgtct gtttccagcc cgtgtaaaac agcgtaaaat cggcaaaccc
     7441 gaatgacctt ccgtccccat caaggagcaa gctatgttct tcaagcacat cgaagccgcc
     7501 cccgccgatc cgattctcgg tttgggcgaa gcattcaaag ccgaaacccg ccccgaaaaa
     7561 gtcaacctcg gcatcggcgt ttataaagac gcatccggcg cgacacccct cgtcaaagcc
     7621 gtcaaagaag ccgaaaaacg cctgttggaa agcgaaacca ccaaaaacta cctcaccatc
     7681 gacggtgttg ccgactacaa cgagcaaacc caaatcctgc tgtttggcaa agaccacgaa
     7741 atcatcgcca gccgccgcgc caaaacagcg caaagccttg gcggtacagg cgcattgcgt
     7801 attgcgaccg agtttgccaa acgtcagttg aacgcgcaaa ccatctggat ttccaatccg
     7861 acttggccaa accacaacgc catcgccaaa gcggtcggta tccaagacaa accttatcgt
     7921 tactatgatg ccgccaaaca cggtttggat tgggacggta tgattgaaga cttgagccaa
     7981 gcgcaaaaag gcgacatcgt cctgctgcac ggctgctgcc acaaccctac cggcatcgac
     8041 cctacgcccg aacaatggga aactctggca aaactttctg ccgaaaaagg ctggctgccg
     8101 ctgtttgact ttgcctacca aggcttcggc aacggtttgg aagaagatgc ctacggcttg
     8161 cgcgtgttct tgaaacacaa tacagaattg ctgattgcca gctcttattc caaaaacttc
     8221 ggtatgtaca acgagcgcgt cggcgcgttc actttggtgg ccgaagatga agaaacagca
     8281 gcccgcgccc acagccaagt caaaaccatc atccgtacct tgtattccaa cccggcttca
     8341 cacggtgcga acaccattgc gctggtgttg aaaaatgatg atttgaaagc acaatggatt
     8401 gccgaactcg atgaaatgcg cggccgcatc aaagccatgc gccaaaaatt tgtcgagttg
     8461 ctcaaagcca aaggtgcaag ccaaaacttt gatttcatta tcgaacaaaa cggtatgttc
     8521 tctttcagcg gattgactcc cgaacaagtc gaccgtttga aaaacgagtt tgccatttat
     8581 gccgtccgct ccggccgcat caacgttgcc ggcattaccg acgacaacat cgattatctg
     8641 tgcgaaagca tcgtgaaagt cttgtaaaac aaaagcgcat acgaaatgcc gtctgaacct
     8701 taaccagttc agacggcatc tgttttttgt taaaaaacaa taaaccataa caatttcaaa
     8761 caagctaaaa acatctataa aatcaaccca atatgatgaa ctcaaataaa aaatacgaat
     8821 atcatcattc tcaaattcaa aatttgaacg ccacagtatt atccgaagcc acaaaaacct
     8881 ttccgccgtc attcccgcgc aggcgggaat ccgggaaccc aatgctgcaa gaattgatta
     8941 gaaacaactg aaaccgagca aactggattc ccgcctgtgc gggaatgacg aaataaggcg
     9001 agtaaaactg atgaaacaaa ttgattaaaa acaataactt atattatttt atttaaattt
     9061 tcgataaaat caattttccg tattcaatac tgctccaatc aattcccgcg caccgtcgtc
     9121 cgccaatttc ttagcgacag cgcgtccgag cgcgtcggca tattcggcag gggcttgcgc
     9181 gtccgcctgc aaaatgaccg aaccgtcggg gtgtccgacc agtccgcgca aggtcagcaa
     9241 gccgttttct tccgtgcaat atgcggccaa aggcacttgg cagcttccgc ccaaagcgcg
     9301 cgccaaagcg cgttcggccg taacgcaggc gtgtgtggta tcgtggttta gcggttttaa
     9361 aacctcatac aaatcttcgc ggtgcgcggc aatttcgata cccaatgcgc cttgtccggc
     9421 ggcaggcagg ctgtcggatt ccgacaaaat catgcggatg cgtccgtcca attccagacg
     9481 ctgcaaaccg gcggcagcca agataattgc gtcgtattcg ccgttatcga gtttagacaa
     9541 acgggtttgc acattgccgc gcaaaggttt gataagcaaa tgcggatagc gcgcacgcaa
     9601 ctgggcttcg cggcgcaggc tggatgtgcc tacgacgacg cctttgggca tttcttccaa
     9661 acgcgcgtat tggttggaca caaacgcgtc aaacggattg gcgcgttcgc cgatggctgc
     9721 aagcgcgaaa ccttcaggca aatccatcgg cacatcttta atcgaatgca cggccaagtc
     9781 agcgcgcccg tcatataaag cctgttccaa ctctttgaca aacaagccct taccgccgac
     9841 ttttgacaaa gttttgtcca aaatctggtc gccgcgcgtg gtcatgccca aaatctcgac
     9901 ttcgcaatcg ggatacaggg ctttcagacg gccttggata tgctttgcct gccacatggc
     9961 aagcaggctt tcgcgactgg cgataacgag ttttttcggg ttcataatgg ttgcctgaaa
    10021 gatggaaaac ggcatgaagt ctatcatatt ttgggcggtc tttcagacgg catgagggtg
    10081 aatatgttat atttacgcct aaaaaccaaa ttttccggac aacatgaaac gcgccaaaca
    10141 atatcctttt ttatccttac agcgacaacg ttttcatctg aactttgaaa acgcttcttc
    10201 caccgccaac cttccgagcg aacgcgattt ctaccgctgg gcgtggtctg ccttgaaaaa
    10261 tgaataccgc cgcgccgaca tcagcctgat tcttctggac gaagaagaag cccgcgctta
    10321 caaccgcgac taccgcggca aagattacgc caccaatgta ttgagtttcg cgctcaacga
    10381 aggcgaaatc ctgccctgcc aagtttcgga aaaactgtac ggcgatttga ttatctgccc
    10441 gcaagtggtt ttgaaagaag ccgccgaaca gggcaaaacg cccgaacggc attttgccca
    10501 cctgaccata cacggcgttt tgcacctgat gggctacgac cacatcgaag acgatgaagc
    10561 cgaaataatg gaagccgaag aaatccgcct gatgctggcg gcaggcttcc ccaaccccta
    10621 ccaagaggac gaatattaaa atggacggta cgcagtcgaa acccaagttt ttcgaacgcc
    10681 tgatctcccg acttgccggc gagcccgact ccgccgaaga cgtattgacc ctgctgcgcc
    10741 aagcgcacga gcaggaagtt tttgatgcgg atacgctttt aaggttggaa aaagtcctcg
    10801 atttttccga tttggaagtg cgcgatgcca tgattacgcg cagccgtatg aacgttttaa
    10861 aagaaaacga cagcatcgag cgcatcaccg cctacgtcat cgataccgcc cattcgcgct
    10921 tccccgtcat cggcgaagac aaagacgaag ttttgggcat tttgcacgcc aaagacctgc
    10981 tcaaatatat gttcaacccc gagcagttcc acctgaaatc cgtcttgcgc cctgccgttt
    11041 tcgttcccga aggcaaatct ttgaccgccc ttttaaaaga gttccgcgaa cagcgcaacc
    11101 acatggcgat tgttatcgac gaatacggcg gcacgtcggg tttggtcacc tttgaagaca
    11161 tcatcgagca aatcgtcggc gaaatcgaag atgagtttga cgaagacgac agtgccgata
    11221 atatccacgc cgtttcttcc gaacgctggc gcatccacgc ggctaccgaa atcgaagaca
    11281 tcaatacctt cttcggcaca gaatacagca gcgaagaagc cgataccatc ggcggcctgg
    11341 tcattcaaga gttagggcat ctgcccgtac gcggcgaaaa agtccttatc ggcggtttgc
    11401 agttcaccgt cgcccgcgcc gacaaccgcc gcctgcacac gctgatggcg acccgcgtga
    11461 agtaagcaga gcctgcccgc accgccgttt ctgcacagtt taggatgacg gtacggacgt
    11521 tttctgtttc aatccgcccc atccgccaaa cataaaaatg ccgtctgaaa ccggaaaaca
    11581 ggttcagacg gcatttcgct tatccgctta ttttttgctc aaagtcagcc ccgtccagcc
    11641 gaacagaagc gtcagtgcca aactcaaata gcagaaaaag gcatacggca gatattccca
    11701 caccggcaca cccagcgcgt ggctgataaa tacgccgcac acgctccacg gaacaagcgg
    11761 gttgatgacc gtacccgcgt cttcgagcgt acgcgagagg ttgcgggaat gtagtccgag
    11821 cctgtcgtaa acgggtttga acgtttctcc cgaaagcagg atgctcaaat attgttcgcc
    11881 aatcaggaaa ttgaccccga ccgaagtcat ggcaacactg aacgtcgcgc gtccggcatt
    11941 cgtcaaaaag ctgcggacgg catccagcag ggaaggaatc gcgcccagcg caaacagcag
    12001 tccacccaaa ctcatcccga gaatcacgat ggtttgcgtg aaaaacatac tttccaaacc
    12061 gccgcgcgaa atcagtttgg caacatcttt aaacgcttcg ccttcgagtt tgtaaccgcc
    12121 gtaaaaccac gcgccgagct gacgcagatc gggcgtgctg tgcagatacg ttacggcaac
    12181 ggcggcaatg acggtaaaga gcatggcgac cacggcattg acgcgcatca atgccaaaac
    12241 gaccaacagt gcaaacggaa tcagcgaata gccgtgcacc aatcccgtgg cttcaagctg
    12301 gctgcggaag gattcgacgc cgttcaaatc ctgcgcggcg acattcggca aaagccaaag
    12361 catcagtgcc gcactaatga gccacgcggg gatggtggtg tacatcatat ttttgatgtg
    12421 ctcaaacaag tcgatgccga cgatggacgc ggaaatgccc gtggtgtcgg aaagcgggga
    12481 cattttgtcg ccaaaaaatg cgcccgaaac aatcgcgccc gccgtcatcg ccatatcggc
    12541 atgaaacgcc gccgccatcc ccataaaggc aacgccgacg gtggcgcagg tggtcaggct
    12601 gctgccgatg gacacgccga tgacggaaca cagcgcgaaa gcggaaaaat aaaaataagt
    12661 cggggaaatc agcccgaaac cgtaatacat cagcgtcgga atcgcaccgc tcatcatcag
    12721 cgcgctgacc atcagcccga tgaagaaaaa caggtaaatc gcgcccatac cctgattcaa
    12781 cgcgcctatc atgccctgct gcatatcgtt gtatttcaaa ccgcgcgcca agccgtacaa
    12841 aatcagcacg acgatggcgg caataatgga catatgcggc agccactcca atgaaatgat
    12901 ggtatagccc atcgcggcaa tcagagcgac gacgacggca agtgcctcac cgcgcggcat
    12961 atcgagtaag gatttgaaag caaacatgat tctttgcccg atttgatata aagttaacgg
    13021 attctaacac accataccga atccgcccaa cgttttcaga cggcatttta gcgttttaca
    13081 aaagccggac agaaacaaaa cggcaatttt ctcaatttaa attaattttt tttgaaatat
    13141 aattttttat ttttttgttt gttggcatat aagactttta tggattgaaa tattaaaacc
    13201 tcggcacaca cgccgcaaac accggtacgc aaaaatgccg tctgaaagat gttcagacga
    13261 catttctcaa gaaaaagatt atttgtccgc cctatatccg tacaccgcaa aaaacacgat
    13321 atacagataa cagatgacgg aaacgacaaa cgaagacatc agggtgtaag tatctgccac
    13381 ccagccctgc acgacaggaa cgaccgcacc gccgacaatc gcggtacaca gtacaccgga
    13441 agccgcgttg gtaaattttc ccaatccttt ggttgccaaa gagaaaatcg tcgggaacat
    13501 aatcgagttg aaaaaaccga tggcaagcag cgaccacatc gccacatccg cattgccgct
    13561 acccgtcgcc atcgcgacgg caagcagtac gaccgcagcc gatgcgttaa acgccaaata
    13621 acggttgggc gcgaatttcg ccatcaccgc cgaaccgagg aaacgtccga ccatcgcgcc
    13681 gccccaatag aacgacaggt aatgcgcggc agaagcatga tccagccctt tcagataacc
    13741 caatacgttg accatcaacg aaccgataga cacctccgcg ccgacatagc agaaaatgcc
    13801 tgccgtaccg aacacgagat gtttgtattg ccatacgctg gttttgccgt cgtggttgtg
    13861 cgcgctttcc tcggcggcaa ttttgcgcgc gtcgggcagc cggatcattt tcacgaaaac
    13921 ggcaaggata atcagcagcc ccgccagtcc caaataggga atctgtacgg aagaaatctg
    13981 ttccgccttg ctgacggttt gggttgcgtc cgccaaaatc aggaacgcgc cgatttgcgg
    14041 cgcaatggtc gtacccaaag cgttaaacgc ctgaaccagc gtcagtgttg ccgattcctt
    14101 gccgggtttc gccagcaggg taacataagg attaccggca acctgaagca gcgttacgcc
    14161 ggaagccaaa ataaacaacg cgcccaaaaa taccgcgtaa gaatggctgc ccgcagcagg
    14221 ataaaacagc aggcatccga ccgccgtcag cagaaagccg ccgataacgc cgtttttgta
    14281 gccgactttg ccgacaaaag cccccatcgg gatggacatc accgcatagg cggtaaagaa
    14341 acagaattgg atcagcatcg cctgaacgta agacaggtcg aaaatttctt tcaaatgagg
    14401 gataaggatg tcgttcatgc aggtaataaa acccatcatg aagaacagcg tggtcaagac
    14461 gaccaatggg gacgtatgat tgttttgtga ttgcgcagac atgatttgcc tttcgtggtg
    14521 tcggaatatt acccgtttca agaaaataga aattgacatt tcttaacggc aatgcaaatc
    14581 atactgatat ttgaacattt tgcaaacttt atcggatgca ggcaatgcgc cgatctgaaa
    14641 ttcaagcagc agaaattaat attttacagt tgcttgcata gattttaata gattttaaaa
    14701 aattccggcg gacttgcaaa aaccgttccc aataaaatga aaatccgccg ccgtatccgg
    14761 aaaattcgga aatgccgcat cgtggcatca aaaccggagc ggaaagaatg tcgtatgccc
    14821 gacaaaaaag gggcatacgg ctgtgccgta ttttttgcac cgtcctccgc ccttcggcgg
    14881 gggcgtgaaa atatagtgga ttaacaaaaa tcaggacaag gcgac
//
LOCUS       DAXHPG010000034        14892 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXHPG010000034 DAXHPG010000000
VERSION     DAXHPG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.034"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            23..1126
                     /gene="dnaN"
                     /locus_tag="ACFM6F_000773"
     CDS             23..1126
                     /gene="dnaN"
                     /locus_tag="ACFM6F_000773"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC6164235.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1095..1364)
                     /locus_tag="ACFM6F_000774"
     CDS             complement(1095..1364)
                     /locus_tag="ACFM6F_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164236.1"
                     /translation="MPSETFPFQTAWFYSNLNRADWNQKHTFAIPAQALPLYAPNPNP
                     PHSRRHCLSDSRTKPRIRLHGSGHISNTGIVLRKTVKSASASQYI"
     gene            complement(1389..3446)
                     /gene="ppk1"
                     /locus_tag="ACFM6F_000775"
     CDS             complement(1389..3446)
                     /gene="ppk1"
                     /locus_tag="ACFM6F_000775"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC6164237.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLNVDEEDVQNLRAAVQNELHDREYGDGVRLEVADTCPARIRD
                     FLLSQFRLTADELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPAVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKIHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIGRIRQETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(3615..4058)
                     /locus_tag="ACFM6F_000776"
     CDS             complement(3615..4058)
                     /locus_tag="ACFM6F_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC6164238.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAINAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(4065..4580)
                     /locus_tag="ACFM6F_000777"
     CDS             complement(4065..4580)
                     /locus_tag="ACFM6F_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6164239.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            4850..7480
                     /gene="leuS"
                     /locus_tag="ACFM6F_000778"
     CDS             4850..7480
                     /gene="leuS"
                     /locus_tag="ACFM6F_000778"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC6164240.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQEFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYNLPKKQVIAVGDNAFDENQWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            7710..8501
                     /locus_tag="ACFM6F_000779"
     CDS             7710..8501
                     /locus_tag="ACFM6F_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC6164241.1"
                     /translation="MNLFFDTKLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCSNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNKHIKDKIRQQLQILRDQNTIEFIGRGLYKKIDKLPPTSKAF"
     gene            8468..8638
                     /locus_tag="ACFM6F_000780"
                     /pseudo
     CDS             8468..8638
                     /locus_tag="ACFM6F_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            8631..8762
                     /locus_tag="ACFM6F_000781"
     CDS             8631..8762
                     /locus_tag="ACFM6F_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HFC6164242.1"
                     /translation="MRNFDKLAEEAAEHAAEGAVKFIDGKPAKKIIAVPGRLANIVV"
     gene            8985..9719
                     /locus_tag="ACFM6F_000782"
     CDS             8985..9719
                     /locus_tag="ACFM6F_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFC6164243.1"
                     /translation="MNAAAAKDYMKTVELNKSAGNVDTTSRTARRVQAVFRRMLPYAD
                     AANNTGHRFDWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDDEIAAIMGHEMTH
                     ALHEHGKNKVGQQILTNTAAQIGTQIILDKKPDTNPELVGLGMDILGTYGLTLPYSRS
                     LEEEADEGGMMLMAQAGYHPAAAVRVWEKMNQENDQNGFIYAITSTHPTNNARIENLK
                     RLLPTVMPVYEQSVRNKGRVNKKRRR"
     gene            complement(10102..10413)
                     /locus_tag="ACFM6F_000783"
     CDS             complement(10102..10413)
                     /locus_tag="ACFM6F_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6164244.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(10410..10778)
                     /locus_tag="ACFM6F_000784"
     CDS             complement(10410..10778)
                     /locus_tag="ACFM6F_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFC6164245.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            complement(11588..11673)
                     /locus_tag="ACFM6F_000785"
     tRNA            complement(11588..11673)
                     /locus_tag="ACFM6F_000785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11637..11639),aa:Leu,seq:gag)
     gene            complement(11687..12037)
                     /gene="secG"
                     /locus_tag="ACFM6F_000786"
     CDS             complement(11687..12037)
                     /gene="secG"
                     /locus_tag="ACFM6F_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC6164246.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(12044..12817)
                     /gene="tpiA"
                     /locus_tag="ACFM6F_000787"
     CDS             complement(12044..12817)
                     /gene="tpiA"
                     /locus_tag="ACFM6F_000787"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC6164247.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            13001..13504
                     /locus_tag="ACFM6F_000788"
     CDS             13001..13504
                     /locus_tag="ACFM6F_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC6164248.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVNA
                     YPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            13606..14262
                     /locus_tag="ACFM6F_000789"
     CDS             13606..14262
                     /locus_tag="ACFM6F_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC6164249.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLASRV
                     VSDDIDAEQQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            14342..14665
                     /locus_tag="ACFM6F_000790"
     CDS             14342..14665
                     /locus_tag="ACFM6F_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6164250.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(14676..>14892)
                     /locus_tag="ACFM6F_000791"
     CDS             complement(14676..>14892)
                     /locus_tag="ACFM6F_000791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164251.1"
                     /translation="SLHIENAAQANFQFASLVLALSHGERGLGCRLPLRFRQILTSLI
                     PRFSFAEAALSDDLLEHHRYTHLRNAV"
BASE COUNT         3352 a         3721 c         4213 g         3606 t
ORIGIN      
        1 aaaccgattt aaaggagcga acatgttgat tttacaagcc gaacgcgaca gcctgctcaa
       61 gccgctgcaa gccgttaccg gcatcgtcga acgccgccac accctgccca tcctgtccaa
      121 cgtcctgatt gagggaaaag gcggtcagac caaactcttg gcaaccgatt tggaaatcca
      181 aatcgacact gcgggtcccg agggcggcgc ggacgacttc cgcatcacca ccaatgccaa
      241 gaaatttcag gacattttgc gcgcgctgcc tgccggtgcg ctggtgtcgc tggattggga
      301 cgacagccgt ctgacgctga aggcgggcaa atcgcgtttt gccctgcaga ccctgcctgc
      361 cgccgatttt ccgatgatga atgtcggcga ggacatcagc gcgactttct cgctggagca
      421 ggagcgtttc aaaaccatgc tgtcgcaagt gcagtacagc atggcggtgc aggacatccg
      481 ctattatctc aacggtctgc tgatgcaggt tgagggcagc cagttgcgcc ttgtggcgac
      541 cgacggacac cgccttgcct atgcagcctg cgcgattgat gcggatttgc cgcgcgccga
      601 agtgattttg ccgcgcaaaa cggtgctgga actgttcaaa ctgttgaaca accccgacga
      661 tccgattcaa atcgagctgc tggacaagca ggtgcgtttc caatgcaacg gcacgaccat
      721 cgtcagcaag gttatcgacg gcaaattccc cgatttcaac cgcgtcatcc ctttggacaa
      781 cgacaagatt ttcgttttgt cccgtgccga acttttgggc gcgctggaac gtgtgtccat
      841 tcttgccaac gaaaaattcc gtggcgcgcg cctgttcctg caatccggcc tgttgagcgt
      901 cgtgtgcagc aacaacgagc aggaagaagc gcgcgaagaa atcgagattg cctatcaggg
      961 cggcgaactc gaagtcggtt tcaatatcgg ctatctgatg gacgtgttgc gcaacatcca
     1021 ttccgacgat atgcagcttg ccttcggcga tgccaaccgc tcgacgctgt ttaccgtgcc
     1081 gaacaatccg aatttcaaat atattgtgat gccgatgcgg atttgacggt tttccggagc
     1141 acgatgcccg tattggagat atgccccgaa ccgtgcagac ggattcgggg ttttgttcgg
     1201 ctgtcggaaa ggcaatgccg tctggaatgc ggcggattgg ggttgggagc gtatagggga
     1261 agtgcttgtg cgggaatggc gaaggtgtgt ttttgattcc agtccgccct gtttagattg
     1321 ctgtaaaacc atgccgtctg aaacggaaag gtttcagacg gcatggtttt tgattggggc
     1381 ggtgcgggtc agcctccgag cagtgtccac aaatcgtttt gcaggtcggc ttcgctttcg
     1441 ccctcggcag gcgcggcgcg gatatagccg ccgtcgggct gcatcagcca ggcgtgggtg
     1501 ttgtctgcca atgccatttc caaaccttcg cggatcacgc gttttttgag ttcgggcgcg
     1561 gtaatcggcg cggcggtttc gatgcggcgg aagaagttgc gccccatcca atccgcgctg
     1621 gaaataaagg tatcgtccgc gccgttgttg tggaagcaat acacgcgcgc gtgttcgagc
     1681 tgcctgccga cgatggagcg gacgcggatg ttttcggaca agcctttcac gccggggcgc
     1741 agagtgcaca taccgcgcac aatcaaatcg atttgcacgc ctgccgcgct tgcccgatac
     1801 agggcttcga tgacggtcgg ttcgatgagc gaattcatct tggcagtaat ccgtgccggc
     1861 ttgcctgctt tggcgtgttc ggtttcctgc ctgatgcgtc cgataaccat tttgtgcagg
     1921 gtaaacgggc tttgatagag cttgttcaga cggccgggtt tgcccaaacc tgtgatttcc
     1981 ataaacaatg tgttcacatc ggcggtgatt tgttcgtcgg cggtaatgat gccgaagtcg
     2041 gtgtagatgc gcgatgtgcc ttggtggtag ttgcccgtgc cgaggtgggc gtaacgcttg
     2101 agcacgccgt cttcgcggcg gatgaccagt gccatcttgg cgtggatttt gtagccgaac
     2161 acgccgtaca cgacgtgcgc gcccgcctct tcgagctgct tcgcccagtt gacgttgttg
     2221 gcttcgtcaa aacgcgccat cagttcgacg acgacggtta cttgtttgcc ggcgagtgcc
     2281 gccttcatca gggcgcggac gagttcggag cgcgtgccgg tgcggtaaat cgtcattttg
     2341 acggcaagca cggcggggtc ggcggcggct tcgcgcatca tttcgacaac ggggtcgaag
     2401 gattggtagg ggtggtgcag caggatgggc gattggcgca ccaaatcgaa tatggacgcg
     2461 gttttgccca aggctttcag cctgcccggc gtgtgcggcg ggaatttcaa atcggggcgg
     2521 ttgaccaaat cggggacggc gttgaggcgc acgaggttca cggggccttt gacttggtaa
     2581 agttcgtcgg cggtcagcct gaattgcgac agcaggaagt cgcggatgcg ggcgggacag
     2641 gtgtcggcga cttcgaggcg cacgccgtcg ccgtattcgc ggtcatgcaa ctcgttttga
     2701 acggcggcgc ggaggttttg cacgtcttct tcatcgacgt tcaagtcgct gtcgcgcgtc
     2761 aggcggaact ggtggcagcc tttgacgttc atgcccggga agagtttgcc gacgtgggcg
     2821 tgcaggatgg aggagaggaa gacgaagccg tgtccgccgc cacacagttc ggacggcagg
     2881 ggaacaacgc gcggcaggat gcgcggtgcc tgcacaatcg ccatccccga aggcctgccg
     2941 aacgcgtccg tgccgtcgag ttcgacggcg aagttgagcg atttgttcag cggtcgcggg
     3001 aaggggtggg acgggtcgag tccgatgggg gtcaggatcg gcagcagttc gcggtcgaaa
     3061 tagtcttcga tccatttttt ctgcgcgccc gtccagttgc ggcggcggta aaaatggatg
     3121 ctttcccgcg ccaactcggg ctgaaggacg ttgttgaaca ggtcgtactg gtgccgtatc
     3181 agggagcgcg ccgcttcggt aacgtcggcg atggtttcag acggcatttt gccgttgtcc
     3241 agcctgcgcc gggggtgcag cttgttttca cgcttgagcc acgccatgcg gacttcgaaa
     3301 aactcgtcaa gattggacga cacgatgcac aggaagcgca ggcgttccaa aagggggacg
     3361 tttttgtctt ccgcctgtgc caacacgcgg cggttgaatg ccagcaggct tagttcgcgg
     3421 cagaggatgc ggttttgttc gggcataagg ttctcccaaa gatggttgtt gttcggtcgg
     3481 agcgggcagg caatgccgtc tgaaggcttg gcggcatctg cgccggtttc agacggcatg
     3541 acgtggcgga cagcctgttt caggcatcgg ggcaatgtga aaacagatac cggcgcgtgc
     3601 ggtatccgtc tgtttcaaat cacttccaac acaaaataat gacgcagttt ttcgtacacc
     3661 gcatcgctga cgttgatgca gccgttggtc ataatcctgt cggacacgga cggggaggcg
     3721 atgcgttcgt tccgcctttc cgacggtatc tgattccaaa cgcggtgcag ggcgaaaaga
     3781 aaatcaccct cctgcttgaa accgatgact tcgccgccgt atccaggctt gtcggtactg
     3841 ttcagcgtca gcccgaacgt ccccttgggc gtggcaacac cgatgaggac ggggtggcac
     3901 tgacggtcgt cagcgaaaca gagttccgct ttggatgtat cgacgatgac ttttttcttt
     3961 tggatatagg cattgatcgc atccggttgc ccctgtgcat atacgggggc aaccattgac
     4021 aggccgcata atatccctaa aaacaggcgg aacggcatgt cggattattg gcggatacgt
     4081 ttcggttttg ccggcacgat ttcgcggatg atgacttgcg gctcggcttt gggtgcgggc
     4141 ggcggacagg cggcatcttt cgggaagacg gggttccagt agaagctgcg ggcaaatttg
     4201 tctttatcga aaatcacttt gtattggcag gtagtaacat cttctacgcc ggaagtgttt
     4261 tcagggtcga tacctacgcc cggggtatgg aagtggaaca ggtaatccca ttcgcgcacg
     4321 ccgtacatac cttcgtcgta atgcgggcgg cccaggattt tgtagatgtc gtctttggtc
     4381 aggccggggc gcatctgatc cagttcgtca taagtcggga atgtgccgcg cttgttgtcg
     4441 agcgttacgg aatagggttt cgggaaaacc ggattgtcgg tcgtgccgtc ggctttgacg
     4501 ttgcttttgg ttgcgcaggc agacagaacg cccgccgcca atactgccaa gcccggtttg
     4561 acgatttgtt tgattttcat atgcagaatc ctcttttacc cgatgcccgt ctgccttttg
     4621 ttcggaagag ccgcatcagg agagttttaa acatttgatt ttggtttgta atattagcat
     4681 aaaaaacgtg cgtatcaata aacgcggtgt atttgtacgg catacggaat aatgcgcgtg
     4741 cgaatttacg catcctgccg gcaatctgcc gattcgccgc ttccggcaac ctgttataat
     4801 tgatgccgac tttatttttc caacgttttt aagcgaaaac agaatgacta tgcaagaaca
     4861 atatcaaccc gccgccatcg agcctgcggc gcagaaaaaa tgggacgacg cccgtatttt
     4921 caacgtctcc gaagacgctt ccaaacccaa atattattgc ctctccatgt tcccctatcc
     4981 cagcggcaag ctgcatatgg ggcatgtgcg caactacacc atcggcgatg tattgagccg
     5041 cttcaaactt ttaaacggct tcaacgtcat gcagcctatg ggttgggacg cgttcggcat
     5101 gccggcggaa aacgcggcga tgaaaaacaa cgtcgcccct gccgcttgga cgtacgataa
     5161 catcgaatac atgaaaaccc agctcaaaag cctgggtttt gcgattgact gggcgcgcga
     5221 aaccgcaacc tgcaaacccg aatactaccg ctgggaacaa tggctgttta ccaagctgtt
     5281 tgaaaaaggc atcgtctatc gcaaaaacgg cacggtaaac tgggatccgg tcgaccaaac
     5341 cgtccttgcc aacgagcaag tcatcgacgg acgcggctgg cgttccggcg cattgatcga
     5401 aaaacgcgaa atcccgatgt attacttcaa aatcacggat tacgccgaag agctgctcaa
     5461 cgacttggac aagctggaac actggccgga acaagtcaaa accatgcagc gcaactggat
     5521 cggtaaatct cgcggcatga ccgtgcgctt cgccgtttca gacgacagca aacaaggttt
     5581 ggaaggcgat tacgcgaaat tcctgcaagt ttacaccacc cgccccgaca cgctgatggg
     5641 cgcgacttat gtcgccgttg ccgccgagca tccgctggca accgccgcag ccgccgacaa
     5701 acctgaattg caagagttta tcgccgaatg caaagccggc tcggtcgccg aagccgatat
     5761 ggcgacgatg gagaaaaaag gcgtgccgac cggccgctac gtcgtcaacc cgctcaacgg
     5821 cgacaagctg gaagtgtgga ttgccaacta tgtattgtgg ggctacggcg acggcgcggt
     5881 gatggcggtt ccggcgcacg acgaacgcga ttttgagttc gccaccaaat acaatctgcc
     5941 gaaaaaacaa gtcattgccg tcggcgacaa cgcatttgac gaaaaccaat ggcaagaatg
     6001 gtacggcgac aaagaaaacg gcgtattggt caacagcggc gacttggacg gcttggattt
     6061 tcagacggca ttcgacgccg tcgccgccaa gctgcaaagc caaggcgcgg gcgaaccgaa
     6121 aacccaatac cgcctgcgcg actggggcat ttcgcgccaa cgctactggg gctgcccgat
     6181 tcccatcgtc cattgcgaac aatgcggcga cgtacccgtc cctgccgacc aattgccggt
     6241 cgttttgcct gaaaacgtcg tacccgacgg catgggttcg ccgctggcaa aaatgcccga
     6301 gttttacgaa actgcctgcc catgctgcgg cggcgcggcg aaacgcgaaa ccgacaccat
     6361 ggacaccttc atggaatcga gctggtattt cttccgctac atgtcgccaa aattttcaga
     6421 cggcatggtc gatcctgccg ccgcgaaata ctggggcgcg gtcgaccaat acatcggcgg
     6481 catcgaacac gcgattttgc acctcttgta cgcgcgtttc ttcaccaaac tgatgcgcga
     6541 cgaaggtcta gtcaatgttg acgaaccgtt tgaacgcctg ctgacgcaag gcatggtcgt
     6601 ttgcgaaacc tactaccgcg aaaacgacaa aggcggcaaa gattggatca accccgccga
     6661 tgtcgagctg acgttcgacg acaaaggccg ccccatttcc gccgtcctca aagccgacgg
     6721 gctgcccgtc gtcatcagcg gcacggaaaa aatgtccaag tccaaaaaca acggcgtcga
     6781 tccgcaagaa ctgattaacg cctacggcgc ggacaccgcc cgcctgttta tgatgttcgc
     6841 cgcaccgccc gaacagtccc tcgaatggag cgacagcggc gtcgaaggcg cacaccgctt
     6901 cctgcgccgc ctgtggcgta ccgtttacga atacttgaag caaggcgaag cggtcaaagc
     6961 gttcgcaggc agccaagacg gtttgtctaa agaactcaaa gacctgcgcc acaaactgca
     7021 cgcgaccact gccaaagtca gcgacgacta cggccgccgc cagcagttca acaccgccat
     7081 cgccgccgtg atggaattgc tcaaccaata cgacaaaacc gacaccggcg gcgaacaagg
     7141 acgcgccgtc gcccaagaag tattggaaac cgccgtacgc ctgttgtggc ccatcgtgcc
     7201 gcacatctgc gaaaccctgt ggagcgaatt gaacggcgcg aaactgtggg aagcaggctg
     7261 gccgacagtc gacgaagccg ccttggtcaa atccgaaatc gaagtgatgg ttcaagtcaa
     7321 cggcaaactg cgcggcaaaa tcaccgtcgc cgccgacgcc tccaaagccg acctcgaagc
     7381 cgccgccctt gccactgaag gcgcggtgaa attcatggaa ggcaagcccg cgaagaaaat
     7441 catcgtcgta ccgggcagac tggtgaacat cgtcgtctaa accgctctta aggtttagcc
     7501 acacggataa aggccgtctg aaacttggaa acagggtttc agacggcctt tttaaggcag
     7561 attgggttgt ctgcattaca gacctcaaat atagtggatt aactttaaac cggtacgacg
     7621 ttgccccgcc ttgccctgct gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
     7681 actaaaactg aaatctcaaa atccaaaaca tgaatttatt tttcgatacc aaattgggaa
     7741 agcaacaaaa taaagcaacc cataaaatcc gtgtaatgag tgaggcttgg ctggaaaaaa
     7801 acggctactg cccctgttgc ggaagcaagc cgatgcagag atttgccaat aacaaacctg
     7861 ttgcagacct cttttgctca aattgccacg agcaatatga attaaagagt aaaaatcaaa
     7921 aaaccatagg taacagtgtg cctgacggtg catatcgcac tatgttggag cgcatccggt
     7981 cagataccaa ccccaacttt ttctttcttg catataaaaa agcggattac tctatacggc
     8041 aattggtgct tgtacccaaa catttcatca cgccggacat gattattccc agaaataaag
     8101 gcattaaaaa ccggccgaac cacattatgt gttccatcaa tctcgcccct ttgcctgaaa
     8161 gcggaaaaat attcttaata gacgattctc gcattatcga acccgaaacc gttctgaaaa
     8221 aatggcaatc caatctgttt ttacgcaacc aaaatgcgga gcgcaaaggc tggcttttgg
     8281 ctgttatgaa atgtatcgac caactccccg aagaattcac attgtcgcaa atgtatgaat
     8341 ttgaaaacaa actatccatc caatttcccc aaaacaagca tattaaagac aaaatccgcc
     8401 aacagttgca aattttgcgc gaccaaaata cgatcgaatt cattggtcgc ggactttaca
     8461 aaaaaatcga caaattgccc ccgacttcca aggcgtttta atttcaaatc atgatactga
     8521 attcacaagg gatatttatt cttctgctat tctgaaaaca gttgaagtac agcgcaatat
     8581 tgccgcaaaa ggcagcagcc gtaaaaaagt cggggaatta ttggcaattt atgcgtaatt
     8641 tcgacaaatt ggctgaagaa gctgccgaac acgccgctga aggcgcggtg aaattcatag
     8701 acggcaagcc tgccaagaaa atcatcgccg taccgggcag actggcgaac atcgtggtgt
     8761 aaatcttttt aaaaacatca aacaatgtta agatgccgtc cgaaatccgt tttagattca
     8821 gacggcattt tttaaattta attttttaag gggtaagcct aatatgaaat ttccttcctt
     8881 ccttccttcc ttcctttaaa cggatactct gcctgtcggc agtaatctcg gtattggggg
     8941 cttgtacggt cgttgctgat gtttacggtc aggattccgc cacaatgaac gctgcggctg
     9001 ccaaagatta tatgaaaacg gttgagttga acaagtctgc cggcaatgtc gataccacat
     9061 ccagaacagc ccgcagggtg caggcagtat ttcgacgtat gctgccttat gccgatgcgg
     9121 caaataatac cggccatagg tttgactgga aaatgacggt tttcaaaaac gatgagctga
     9181 acgcgtgggc gatgcccggt ggaaaaatgg cgttttatac ggggatagtc gacaaactca
     9241 agctgaccga tgacgaaatt gccgccatta tggggcatga aatgacgcac gccctgcatg
     9301 aacacggtaa aaataaggtc gggcagcaaa tcttgaccaa tacggcggcg cagataggca
     9361 cgcagattat attagacaaa aaaccggata ctaatccgga attggtcgga ttgggtatgg
     9421 atattttggg gacgtacggt cttaccttgc cttatagccg cagcttggaa gaagaagccg
     9481 atgagggggg aatgatgttg atggcgcagg caggctatca tccggcggcc gctgtcaggg
     9541 tttgggaaaa aatgaatcag gaaaacgacc aaaacggctt tatttatgct attacctcta
     9601 ctcatccgac aaacaatgcc cgtatagaaa atctaaaacg gttgttgccg accgttatgc
     9661 cggtttatga gcaaagtgtc agaaataagg ggcgcgttaa taaaaaacgt cggcgttaag
     9721 ggaaggggat cgagaatttg agccattgtt tcaaaatgcc gtctgaaatc gtttgagatt
     9781 cagactgcat ttaaacagtt ctgtgcccca cccgttttgc cttcaagccc gcgccgctta
     9841 aatccaatcc ccccctgcaa cgggcgcaaa aaatccccgt acctcgcggt cggtgatgtt
     9901 tcgggtttcg cgcccgtctg cgccgtgcct tgctgtcggg ctatgtcgtc tgcaccgccg
     9961 ccgtttcggc ttggtttgat gcggtcgggc atttatgcgg ctacgccctg atattcagtt
    10021 cggcggtctt gcctgtcttg gcaggcatat tcagcagcgc gtctattgtg aacttgctga
    10081 ttttgccgtt tgccaagtcg gctacgcaat ggcttcgatg cccttgtttt tgacgatggt
    10141 cagcagcttc aacgccgcgc cgctcggctt tttaacgccc cgctcccaag ccgaaacgtg
    10201 attttttccc acgttgagat agatggcgaa agcggcttgc gatagtgcct ccttctccct
    10261 gattgccttg atgtccccgc cgctcaacgg tttgatttcg gtcaggcagg acttgtcaaa
    10321 gccgcgcata gtctttttgt cgattgcgcc gatgttgtaa gccccttcca tcatttcatg
    10381 aatggcggct aatgcctcgc ttttgtattt catgattcta tctccgtgat aatgccgtct
    10441 tctttcaaag ccgccagctc tgcccgcgtg tatttcagat aatatgcggc ggcttttcgg
    10501 taaacgtcca gttctttatc cgaaatgttt tccctgtcgt tcttggcaaa ggcgtaaacg
    10561 aaaaatgccc tatctgcctg tttgaacaat atcaggctgc gatagccgcc gcttctgcct
    10621 tgtccttgcc ttgctatgcg ctgcttaatc acaccgccgc ctaaatctgc gtctatcagc
    10681 ccgttatccg cccgctctac cgcttccagc agctcggaat cgttgatttt gtgcttcttg
    10741 gcaaatttca ctatccattg gtttttgaat atccgcattg tgtagccgtt gattagtgat
    10801 acttggaatt ataccagaga caggcaaggc ggcaagtcgc ctttttatac cctgcaattt
    10861 ctccgtttat gccgcctgaa acaccggcgg ctaacctgat tattttttaa tcaatcgctt
    10921 ttaatggctc aaattgcgtt tttagcgcgt ttcgctgtct tcctatgcca gtatataacc
    10981 aagcggttta accgccattc ctgctcaaaa taggcgcggt tccgtgcgtt ttggcttttc
    11041 attccgctac gctcccactg ccaagacggt gaaaaaccgg aaaaccccaa aagttgttac
    11101 cgattcgtta ccatttgtta ccgcatttgt taccaatttt ttatcttttt cttattttat
    11161 agtggattaa atttaaaccg gtacggcaag gcgaggcaac gccgtaccgg tttaaattta
    11221 atccactata gtataggttt tttccaaaaa cccaaaaaaa accgctctgc atcttggacg
    11281 gtttccccct cattccctac ggtgcgtcag tattccccgc aagctatttc ccgcttggca
    11341 ttcctgattt ggtggtcgtt cagctttatt tttgcggtat tttttcgagg tatttttcag
    11401 ataccgcaaa agataccgta aattttaggt tacttcaatt aggctgaatt aaacggaatt
    11461 atacaatcag gaagaagaca ataggatagc aaccagtctg aaagccttgt attgattggg
    11521 attggttgac ggcattagac aggattaaac gaaaaaaacg cttagaattt ctaagcgttt
    11581 tgtgtgttgg tgccgacagc gagatttgaa ctcgcacagc ctacggccac taccccctca
    11641 agatagcgtg tctaccaatt tcaccatgtc ggcatttgaa aaactgttat ttctgctgct
    11701 gaggaacagg ggcagaaggt tcggtattgc ttacgggttt gggtgcttgc tgagtttgtt
    11761 gtacgttgct gaagtccaaa ccgtgttttg ttgtgtgggt gtgaatatac accatagcca
    11821 tgcaggttgc aaagaaaaat gttgctgcaa cggcggtcga gcggctgagg aagttagcgt
    11881 tgccggcaga gccgaatacg ccttgcgcgc tgccgcttcc cgatccgaaa gtcgcgccgg
    11941 catccgcgcc tttgccgtgt tggagcaata ctaacacgat gacggccaaa gcggaaatta
    12001 tattaacaat ccaaattagg gttttgaagg cttccatatt tttctacgca ttttgtgcgg
    12061 cactgatgat ggcggtaaag gagtcgtacg acaatgacgc gccgccgacg agtgcgccgt
    12121 ccacataagg tactgcgaag atgtcggccg cgttgtccgc tttcacactt ccgccgtaaa
    12181 ggacgcggat tttaacatcg cttccgcaca aagacaagat ttctttgtag atgaatgcgt
    12241 gcatatcggc aatctgttcg acggtggcga ctttgcctgt gccgatcgcc cagacgggtt
    12301 cgtaggcgac ggcgatgttt ttggtgtcca gtccttggag gatagaaagc tgatgggcga
    12361 tgacttcgtg ttctttgccg gcttcgcgct cttcgaggct ttcgccgacg cacaataacg
    12421 ggatgagtcc gacgttgagg acgttttcca ttttgcggcg ttggatttcg tttttttcgc
    12481 cgaaataaag gctgcgttcg gagtgtccga tgaggacgat gtctgtgccg gtgtcggcga
    12541 gcatttcggc ggacacttcg ccggtgtacg cgccgttgtc ggggaagcgg ctcacgtctt
    12601 gggcgcaggt gaggatgcgg ttgtttaaga cgatttgcat ggcgttgtgc agttgcagca
    12661 ggtaaacggt cggggcggcg agtccgatga ggacgcgttc ggcggtgggg aggatgcgga
    12721 agcggtgcat gagcgaattg ttgttctgga gccggccgtt cattttccag ttgccgatga
    12781 cccatttttg atcccacatt ccgatttggt gatacatctt ttttgctccg tgtcgtattt
    12841 tttctgtctg ccgcgtgtgg cgcgttgcaa tgtgaagttt agtggatatg cggcgggttc
    12901 gcaacttggg gcgggcggct gcgggggcgg tttggaatgt tgtttcgggc aggttgtttt
    12961 ataatggccg cctgatatgt atgcaactat aggagatgtg atgcacgcgc ttcatttttc
    13021 ggcttcggac aaggccgcgc tttatcggga ggtgttgccg cagattgagt ctgtggtggc
    13081 ggacgagacg gattgggtgg cgaatttggc gaacacggcg gcggttttga aggaggcgtt
    13141 cggttggttt tgggtgggtt tttatttggt cgatacgcgt tcggacgaat tggttttggc
    13201 accgtttcag gggcctttgg cgtgtacgcg gattccgttc ggacgcgggg tgtgcggtca
    13261 ggcttgggcg aagggcggga cggtggtcgt cggggatgtc aacgcgtatc ccgaccatat
    13321 tgcctgttcg tctttgtcgc gttcggagat tgtcgtgccg ctgttttcag acggccgctg
    13381 tatcggcgtg ttggacgcgg acagcgaaca tttggcgcag tttgatgaga cagatgcttt
    13441 gtatttgggc gagctggcga agattttgga gaagcggttt gaggcttcgc gtcaggcggt
    13501 ttgagtgagg gaaaggggcg ggcggcgcgt gccgaagttg gcgcggcggg agtgtggttt
    13561 tataatgcct gctattgttg gaacaattat ttgacggagc actaaatgga ttttgaaaaa
    13621 gcgcggttca atatggtcga acagcaaatc cgtccgtggg atgtattgga ttttgacgtt
    13681 ttggacgctt tggcggagat tccgcgcgag ctttttgtcg atgaggactt gcagggtttg
    13741 gcgtatgcgg atatggcgct gccgcttgcc aacggtcata agatgctgga gccgaaagtc
    13801 gtggcgcggc tggcgcaggg cttgaagctg acgaaaaacg atacggtttt ggaaatcggc
    13861 acgggttcgg gctatgcgac cgcgctgttg gcaaaattgg ctagtcgtgt ggtttcggac
    13921 gacatcgatg ccgaacagca aaagcgcgcc aaagcggttt tggacggctt gtctttggaa
    13981 aacattgatt atgtgcaaaa caacgggctg accgaacttt ccgcaggcgc gccgtttgat
    14041 gcggtttatg tcggcggcgc ggtaaacctt gtgcctgaag tgttgaaaga acagttgaaa
    14101 gatggcggac gcatggcggt tatcgtaggc cgcaagcctg ttcagcgcgc gcttttgatt
    14161 acgcgcaagg gcgatgtgtt tgaagagaag gtgctgttcg atactttggt ggcgcatttg
    14221 gatgataagg atgccgatcc gtttggcggt ttcgattttt aagatgttga caggtcatgc
    14281 cgtctgaaag cgggtttggg gcttcagacg gcattttgct tggttttttt gggggggtgc
    14341 gatggatatt gtgcaacttg ggcccgcgga attgaaagcg tggatggacg aagggcggat
    14401 gttttgtttg ttggacgtgc gtacggatga agaaacggcg gtttgttcgc tgccaaatgc
    14461 gctgcatatc ccgatgaatc tgattccgct gcggcagaac gagctgccgg atgatgtgcc
    14521 gcttgtggtg tattgccatc acggtatccg cagtctgcat acggcgatgt atttggcgga
    14581 cgcgggtttt gaaaacctgt acaaccttca gggcggcatc gatgcatggg cggccgagat
    14641 tgacggcaac atgatgcgtt attgaggcag gcatttcaga cggcattcct taaatgcgtg
    14701 tacctgtggt gttccaaaag gtcgtctgaa agtgcagctt ctgcgaagct aaagcgcggt
    14761 atcaacgaag ttaaaatttg cctgaacctt aaaggcagcc tgcaccccaa tcccctctcc
    14821 ccgtgggaga gggctaggac gaggctggca aactgaaagt ttgcttgggc ggcattttca
    14881 atgtgcaggc tg
//
LOCUS       DAXHPG010000035        14775 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXHPG010000035 DAXHPG010000000
VERSION     DAXHPG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14775
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.035"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            43..675
                     /locus_tag="ACFM6F_000792"
     CDS             43..675
                     /locus_tag="ACFM6F_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC6164252.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="ACFM6F_000793"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="ACFM6F_000793"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC6164253.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQVQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGSLEIPVEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2197..4065
                     /locus_tag="ACFM6F_000794"
     CDS             2197..4065
                     /locus_tag="ACFM6F_000794"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6164254.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVEFSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
     gene            complement(4160..4773)
                     /locus_tag="ACFM6F_000795"
                     /pseudo
     CDS             complement(4160..4773)
                     /locus_tag="ACFM6F_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            complement(4810..6960)
                     /gene="dinG"
                     /locus_tag="ACFM6F_000796"
     CDS             complement(4810..6960)
                     /gene="dinG"
                     /locus_tag="ACFM6F_000796"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC6164255.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            7117..7287
                     /locus_tag="ACFM6F_000797"
     CDS             7117..7287
                     /locus_tag="ACFM6F_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164256.1"
                     /translation="MLLYRLLGGAEIECGRCPVPPMTDWQHFLPAMGTVSAWVAVIWA
                     YLIIESEKNGRY"
     gene            complement(7355..8656)
                     /locus_tag="ACFM6F_000798"
     CDS             complement(7355..8656)
                     /locus_tag="ACFM6F_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC6164257.1"
                     /translation="MPSETARPLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPNLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDSPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(8646..9557)
                     /locus_tag="ACFM6F_000799"
     CDS             complement(8646..9557)
                     /locus_tag="ACFM6F_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6164258.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLIRNGVPKTPEEVTRLPAVLPTYTNQQKLDITHKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAVEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            9665..10063
                     /locus_tag="ACFM6F_000800"
     CDS             9665..10063
                     /locus_tag="ACFM6F_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6164259.1"
                     /translation="MSNCCNRIQPVLLSVLRIVTAYLFLLHGTSKFFAFPIEMGSGSP
                     GGLMLLAGILEVVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFFYIAAAGGGAWSLDRLFFKRKA"
     gene            10113..10718
                     /locus_tag="ACFM6F_000801"
     CDS             10113..10718
                     /locus_tag="ACFM6F_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6164260.1"
                     /translation="MTRQSLQQAAESRRSVYLLNKNLPIGKDEIVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            10904..13015
                     /locus_tag="ACFM6F_000802"
                     /pseudo
     CDS             10904..13015
                     /locus_tag="ACFM6F_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            13213..13872
                     /locus_tag="ACFM6F_000803"
     CDS             13213..13872
                     /locus_tag="ACFM6F_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFC6164261.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            14043..14738
                     /locus_tag="ACFM6F_000804"
     CDS             14043..14738
                     /locus_tag="ACFM6F_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6164262.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPAP
                     LTEGVNYTVLSTPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEASESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         3336 a         3809 c         4095 g         3535 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcggtc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 atggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttccgaa tacggcctga
      841 tgaaagcgcg cgtcaaagtc gaattaaact ggctcaaagc cctcgccgcc gagccgaaga
      901 ttgccgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccacgacc aatcatgatg
     1021 tcaaagccat tgaatattgg ctgaaaaaac gttttgccga agtgccggaa gtcgccgccg
     1081 tgagtgagtt catccacttc gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa gctggccgaa atcatcgaaa
     1201 aactgaccgc tatggcgcac gaccttgccg ccgtcccgat gatgagccgc acccacggcc
     1261 agcccgccac gccgaccact ttgggcaaag aaaccgccaa cgtcgtttac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gtgcaagagt tcctcggcaa aatcaacggc gcggtcggca
     1381 actacaacgc ccatatggtt gcttatcccg atgtggattg ggaaacccac tgccgcaact
     1441 ttgtcgaaat cagcctcggt ctgaccttca acccctacac catccaaatc gaaccgcacg
     1501 actatatggc ggaattcttc caaaccctca gccgcatcaa cacgattctc atcgacttta
     1561 accgcgacgt ttggggttat atttcattgg gttacttcaa acaaaaagtc aaagcgggcg
     1621 aagtcggttc ttccaccatg ccgcacaaag tcaaccccat cgactttgaa aactccgaag
     1681 gaaacctcgg tatggcaaac gccgtattgg gctttttgtc cgaaaaactg ccgatttccc
     1741 gttggcagcg cgacctgacc gacagcaccg tcttacgcaa tatgggcgtg ggcgtgggct
     1801 atgccgtgtt gggtttcgcc gcacacctgc gcggtctgaa caagctcgaa cccaaccctg
     1861 ccgcgcttgc cgccgatttg gatgccactt gggaactgct cgccgagccg attcaaaccg
     1921 tgatgcgccg ttacggtgtc gccaatcctt acgaaaaact gaaagacctg acgcgcggca
     1981 aaggcggcat tacgcccgaa gtgctgaaag gctttatcgg atcgctggaa atccccgtcg
     2041 aagccaaagc aaaattgctt gagctgaccc ccgcactgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacggggcg atatttatgc aagctgtccg atacaggcct
     2221 gaaattgacg gattgcgggc cgttgccgtg ctatccgtca ttattttcca cctgaataac
     2281 cgctggctgc ccggagggtt tttgggggtg gacattttct ttgtcatctc gggattcctc
     2341 attaccggca tcattctttc tgaaatacag aacggttctt tttctttccg ggatttttat
     2401 acccgcagga ttaagcggat ttaccctgcc tttattgcgg ccgtgtcgct ggcttcggtg
     2461 attgcctctc aaatcttcct ttacgaagat ttcaaccaaa tgcggaaaac cgtggagttt
     2521 tctgcggttt tcttgtccaa tatttatctg gggtttcagc aggggtattt cgatttgagt
     2581 gccgacgaga accccgtact gcatatctgg tctttggcag tagaggaaca gtattacctc
     2641 ctgtatcctc ttttgctgat attttgctgc aaaaaaacaa aatcgctacg ggtgctgcgt
     2701 aacatcagca tcatcctatt tctgattttg actgccacat cgtttttgcc aagcgggttt
     2761 tataccgata ttctcaacca acccaatact tattaccttt cgacactgag gtttcccgag
     2821 ctgttggcag gttcgctgct ggcggtttac gggcaaacgc aaaacggcag acggcaaaca
     2881 gcaaatggaa aacggcagtt gctttcatca ctctgcttcg gcgcattgct tgcctgcctg
     2941 ttcgtgattg acaaacacaa tccgtttatc ccgggaatga ccctgctcct tccctgcctg
     3001 ctgacggcgc tgcttatccg gagtatgcaa tacgggacac ttccgacccg catcctgtcg
     3061 gcaagcccca tcgtatttgt cggcaaaatc tcttattccc tatacctgta ccattggatt
     3121 tttattgctt tcgcccatta cattacaggc gacaaacagc tcggactgcc tgccgtatcg
     3181 gcggttgccg cgttgacggc cggattttcc ctgttgagtt attatttgat tgaacagccg
     3241 cttagaaaac ggaagatgac cttcaaaaag gcatttttct gcctctatct cgccccgtcc
     3301 ctgatacttg tcggttacaa cctgtacgca agggggatat tgaaacagga acacctccgc
     3361 ccgttgcccg gcgcgcccct tgctgcggaa aatcattttc cggaaaccgt cctgaccctc
     3421 ggcgactcgc acgccggaca cctgcggggg tttctggatt atgtcggcag ccgggaaggg
     3481 tggaaagcca aaatcctgtc cctcgattcg gagtgtttgg tttgggtaga tgagaagctg
     3541 gcagacaacc cgttatgtcg aaaataccgg gatgaagttg aaaaagccga agccgttttc
     3601 attgcccaat tctatgattt gaggatgggc ggccagcccg tgccgagatt tgaagcgcaa
     3661 tccttcctaa tacccgggtt ccaagcccga ttcagggaaa ccgtcaaaag gatagccgcc
     3721 gtcaaacccg tctatgtttt tgcaaacaac acatcaatca gccgttcgcc cctgagggag
     3781 gaaaaattga aaagatttgc cgcaaaccaa tatctccgcc ccattcaggc tatgggcgac
     3841 atcggcaaga gcaatcaggc ggtctttgat ttgattaaag atattcccaa tgtgcattgg
     3901 gtggacgcac aaaaatacct gcccaaaaac acggtcgaaa tatacggccg ctatctttac
     3961 ggcgaccaag accacctgac ctatttcggt tcttattata tggggcggga atttcacaaa
     4021 cacgaacgcc tgcttaaatc ttctcgcgac ggcgcattgc agtagcctgc cttgccgtcc
     4081 gatatcgttt gtgccgccgt ttgcctttcg gggcggcggc ggtttttatt ttccttcccc
     4141 tgcgggaggg aattttgaat caaaacccca attgcctttc caagttttcc accagattgt
     4201 catccagttc caaagcctgc gacaggcggg cgaggaagac ggtttctttc cgcgacaaat
     4261 cggcacatac cagtcttgcc gccagatagg cctccgccgc caacgcctca tcgttgccga
     4321 cggcggcggc gatgtcttcg atgcttgcgg gcaggcggta ttcggcggcg agccatgcag
     4381 cagtttcggg gtctgtgccg ctttcctgtt cgatagtccg gcgttcggct tcgtctatca
     4441 tgccgtctga agcggcggtg gctatcatgg tgcgcaatac ggtgcggctg tatgtttctt
     4501 cggtttctcc ggcaggttgg aaatcgcttt gtgttacggt tgcccgccct ttgttttgct
     4561 gccacatctg atagccccgg taggcgaggt agcccaaagc ggcggtcgaa ccgattttgg
     4621 tgatggtttt gcggttttta ccgttcagca gcatggaggc gacaccggca accagcgcgc
     4681 ctccgccgaa tgaattgagc gggctgtcgg agaatgtgtt gccttttttt gaaccgtgct
     4741 taagacttgg ttgagcagtc gggtaaagtt catgaatttt tcctttctgt tgcgggaagg
     4801 gcggtatgtt taccctatcc ttttaaacgg cggcaggccg gccaataatt gttggccgta
     4861 ccgctgtgtt ttgatgcggt tgtcgaggat ggttacgcgg ccgtagtctt gttcggtgcg
     4921 gatgaggcgg ccgacggcct ggatgagttt gatgccggct tcggggacgg tgatttcaat
     4981 aaaaggattg ccgccgcgct gttctatcca gcggttttgg gttttttcga tggggttgtc
     5041 aggcatcgca aacggcagct tggcgatgat gacttgcacg caggcggtgc cgggcaggtc
     5101 gagtccttcg gcgaaactgt cgagtccgaa gatgatgctg gctttgcctt cttctatggc
     5161 ccggtggtgt ttttgcagga ggacggcttt gggtaattcg ccttgtacga gcaagagcgg
     5221 caggtagtct ccgggcaggc gcagggcgac atcctgcatt tgtttgcgcg aggaaaacaa
     5281 gacgagcgtg ccgatggctt cggtgggcga aataagcttg ggcagccatt cgatgacggc
     5341 ggcggtgtgg gcttcggggt ctttggggct ggcgtatatg ggggggatgt agagttcgcc
     5401 ctgtttttca aagtcaaagg ggcttttgag ggcgagggtg gtggtttcgg gcagccattg
     5461 cagcccggtt tggcgcagca tcaggttgaa gttgcccaag gattgcaggg tggcggaagt
     5521 caataccgcg cctgccgcac gccgccacag gctgttggca aggtgggatg cgctgctgat
     5581 ggggctggcg ttgaaaatgt agtcgttttt gtcgtcggca cggcgggtta tccattttgc
     5641 cagcggctct tcgccttcga gggggacggt ggagagcaaa tcccaaaccg cgctgatttg
     5701 ttcgatacgg acgatgaaga tgccgaactc gccggtcagg cggtcgagga gcgcgccgtc
     5761 ctgttctttt tcgcggcgcg cggcggaaag cgcatcgttc agcccgacaa cgtgtttgag
     5821 caggctgcgc gcagcaatgg ccgtattgga aacggtggtt tcgaggcctt cggggatttt
     5881 gccgtcttcc cacagccaag tcggttcgct gttggttcgt ctgtcgtttt cagacggccc
     5941 catacttaaa gacggctctt ccgccaaatg gaactgccat tcgtgcaggc tgtcgagcaa
     6001 ggatgcggcg gcttcgtcgg ccaggttggc aagttcggct ttatcggtaa gcgcggcaat
     6061 tttgccggtc agctgcggca gtttttccag cgtccaaacg gcaatattcc atgaatgttc
     6121 ggcggcaaaa cggctgaggg cttttttggg caggtggtgc gcttcgtcta tgcaatagaa
     6181 actgttttcg ggcgcaggca gaatcacgcc gccgcccata ctgatgtcgg caagcagaag
     6241 atcgtggttg gcaacgacga catcgacggt ttccaagaca tcgcgtgcta ggtaaaacgg
     6301 acattccgga cggttgggac aggcggtttt caggcagccg tggcggtcgt tggtcacttt
     6361 gagccaaatc gcgtcatcga ttttttccgg ccaagtgtcg cggtcgccgt tgaaccgtcg
     6421 ggcggaaaat tcgtcggcga tgtcgcgcag cagcttcaat tcttcgggct tgggtttgct
     6481 gtcccacaag acggcggggg cttcaaagcc gagcaggttt tgctgggcat tgctttgcgt
     6541 cagttgatag agtttgtagg ggcagagata gcggccgcgc cctttggcaa gcgcgaagct
     6601 cagttccaaa ccgctttttt cgaccaaaaa cggcaggtcg cggtctacca actgctcctg
     6661 caaggcaacc gttgcgctgc tcacaatcag ccgcttgccg cgcgtctgcg ccatgatgcc
     6721 gcccgccagc aggtaggcaa gcgatttgcc cacgccggtc ggcccttcga ttacggcgat
     6781 gctctcgccc tcgcgcttgg gcggctcgcc gccttcttcg cgcgccaacg tccgcgaaaa
     6841 agcgttggca accgccgcaa tcatttcccg ctgcgaagcg cgcggacgga aaccgggcag
     6901 gtttttgccg atgttttggt aatggtcgcg gatggcgttt ttttctaaat cggtgagcat
     6961 ggcgttttgt acggcggtag aagtgggctt attttaacat tgcacggaag cggtacaata
     7021 tcgttgtcgg aatggggggt gaggtgaatc gtgcggacgt ggttggtttt ttggttgcag
     7081 cgtttgaaat acccgttgtt gctttggatt gcggatatgt tgctgtaccg gttgttgggc
     7141 ggcgcggaaa tcgaatgcgg ccgttgccct gtgccgccga tgacggattg gcagcatttt
     7201 ttgccggcga tgggaacggt gtcggcttgg gtggcggtga tttgggcata tctgattatt
     7261 gaaagtgaaa aaaacggaag atattgagtc atccggacgc aatgccgtct gaaacggaag
     7321 ttcagacggc atttgtttta ggttgccgta ccgcttaggg aataccggcg acaggatagg
     7381 cgggatagcc gtgggtatcg accgaacagg caaaccgcca aggcgtgtgg acggtgtcgg
     7441 cggacaggtg ggcgagttcg ggaatgtgcc gtttgataaa agtaccgtcc gggtcggtac
     7501 ggtatgaggc ggcggcaatg tcggggcagg tgtgccgtga ggcggcaagc caccagttgc
     7561 cttggttgat tgccgcatcg aaatcggtca gctgtcgggc aaaccatatc tcgccttcgc
     7621 ggcgggggag gtttaaaacg tggcagaaaa aatccgcgct caagcgtctc agggcggggt
     7681 ggaggctgcc ggttttgtgc aaacagcgca tcgcggcatc gataatcgga atgccggtct
     7741 ggccctgctg ccaaagcgtc aggcgcaggg tgtgttccgg attgccgtct gaagggctgt
     7801 catccgtgtg ctgcaaggca agttgaagga aaaaatcgcg gcggatgatg ttgtccgccc
     7861 acgcgttcag acggcgttcg aggctttccc gcgcgagcag gcgcggcgag atgcagccgg
     7921 cactcaaata cgcgcccatc agcgaagtgt gtttgcgcga ggggaaatcc tttaaaacgg
     7981 agtaggaatc cgcctgttcg agaaaccgcc gccactgccg ccaagccgcc gtttcgccgc
     8041 tgttttgcgg caggaagatg ccgtctgaaa gggcggcagg cggcggggcg gaaaggtttt
     8101 cggggaaggg ttggcggtat gccgcgaata ggttcggacc ggcggggggc tgcttggaaa
     8161 agcggtcgag ccatacttcg cggtagcggt cgaaatcggt gtgtgccgtg ccgccgtcgg
     8221 gtatcaggtc ggttttgccg aaaacggcgc ggtcattgac gaaggttaac gcgatgccgt
     8281 gtttgtccaa ttcatgccaa agggcgttgt cggcgagttt gtcggcaaaa gtatgggatt
     8341 cgtcggcgat gacggcgcgg atattgaggc ggacggcgag ccggacgagc tcggcaggcg
     8401 atgccgccgt gtagagcggg atgccgcgcc ctgcaagccc ttgggcgagt tcggcggcgg
     8461 attggcggta gaacgcggcg cggcgagggt tgtctgtttc ggcatcgtca atccaaatac
     8521 cgataacggg caaaccttcg gcaacggcgg cgcataaggc ggcgttgtcg cggatgcgga
     8581 ggttttggcg gaaccagacg agcgtgtgtg cggcgcgcgt gtccgcataa agggggcggg
     8641 cggtttcaga cggcatttcg gcagcctttc ctgctggcga ttttttcgtt cagaaaatcg
     8701 atgaagctgc ggactttcgc gcttaagaat gccctgtctg cataaacggc attcagccgg
     8761 tcggtcggga cggcgtatcc gggcagcagc ctcaccagcg tgccgcagcg caaatcgtgt
     8821 tccaccgccc aaagcggctg ataaccgatg cacgcgcccg ccttaatcat ttcgcgcatc
     8881 atcagcgtgt tgtcggtacg gatgacgggg gtcagctcaa gccggtattt tttgccgtcc
     8941 gatttgtggg tgatgtcgag tttctgctga ttggtgtagg tcggcaggac ggcgggcagc
     9001 ctcgttactt cttccggcgt tttcggtacg ccgttgcgta tcaggaagtc gggcgaggcg
     9061 agcagggcaa attcgatttc cgccagcggg cgcgcaatca gcgacgggga cagggtttgg
     9121 gaaacgcgca acgccaaatc cacgccttcg gcaatcaaat cgacgtgccg gttgtccaaa
     9181 atcagttcta atgttacttc gggataacgt tcgcggtatt ccgccagcca gttgcatatc
     9241 tggctgccgg caaaccacag cggcatcgtt acgcgcagca gcccctgcgg tttttccgtc
     9301 ccccctgcgg ctttttgcgc ggcatcgtcg agcgtgtcga gcgcgtaact gcattgtcgg
     9361 tagtattctt cccccgcttc ggtcaggctg agattgcggc tgttgcggtg caggagtttg
     9421 gcttggacgg tgttttccaa gtggctgacg tgtttgcttg ccattgcggt ggagatgccg
     9481 agcgcgtcgg cggcgcgggt aaagccgccg ctttggacga cttggcggaa aactttgagg
     9541 ctgaacaggg tgtccatatt ttcttgtgtg gaaaagttgt atcaataaaa gcagtatata
     9601 tttgaaaagg ggaaacatct atactctgcc gcctgaaatg aagataaata ccgaaggagc
     9661 ttttatgtcc aattgctgca accgtatcca acctgttctg ctttctgttt tgcgtatcgt
     9721 aaccgcctac ctatttttgt tgcacggtac gtcaaaattc ttcgccttcc ccattgaaat
     9781 gggcagtggt tcgcccggcg ggctgatgct gcttgccggt attttggaag tcgtcggcgg
     9841 cattttgctg gtgttgggcc tgtttgcgcg ccctgccgcg tttgttttgt ccggccagat
     9901 ggcggttgcc tattttatgg cgcacgcttc cggaaatgct ttgtttccga ttgccaacgg
     9961 cggcgagtcc gcggtgctgt tctgcttcgt attcttctat atcgcggcgg cgggcggcgg
    10021 cgcgtggtcg ctggacaggc tgtttttcaa gcgtaaagcc tgaactatgc cgtctgaagc
    10081 gtattttgcg aatatttttg aaaggaaaag aaatgacccg tcaatccctg caacaggctg
    10141 cggaaagccg ccgttccgtt tatttgctaa ataaaaatct gcccatcggc aaagatgaaa
    10201 tcgtccaaat cgtcgaacac gccgttttgc acacgccttc ttcgtttaat tcccaatctg
    10261 cccgcgtggt cgtgctgttt ggcgaagagc atgataaggt gtggcaattt gtcgaagacg
    10321 cgctgcgtgc cgtcgtgcct gccgacagtt ttgaaccgac cgcgcaaaaa ttgaacctgt
    10381 ttaaggcggg tgcggcaact attttgtttt atgaagatca aaatgttgtc aaaggtttgc
    10441 aggagcagtt ccctgcttat gccgccaact ttcccgtttg ggcggaccag gcgaacgcta
    10501 tggtgcagta tgccgtttgg acgacacttg ccgcggtcgg cgtaggtgca aacctgcaac
    10561 attacaatcc cttgcccgat gcggcgattg ccaaagcgtg gaatatcccc gaaaactggt
    10621 tgttgcgcgc acaaatggtt atcggcggta ttgaaggggc ggcaggtgaa aagacctttg
    10681 aacccgttgc agaacgtttg aaagtgttcg gcgcataatt tcgcggtcaa aaaaatgccg
    10741 tctgaaccct gttcagacgg catttttcag tatcaggcgg cgagttttcc gcattctgag
    10801 acctttgttt acaaatatca tgttcaatat agttaaaaga aattattctc atttcctccg
    10861 tgaggcaata taattcggtt gttttgttaa attgagtata aaaatgaaaa tatcatttca
    10921 tttagcttta ttacccacgc tgattattgc ttccttccct gttgctgccg ccgatacgca
    10981 ggacaatggt gaacattaca ccgccactct gcccaccgtt tccgtggtcg gacagtccga
    11041 caccagcgta ctcaaaggct acatcaacta cgacgaagcc gccgttaccc gcaacggaca
    11101 gctcatcaaa gaaacgccgc aaaccatcga tacgctcaat atccagaaaa acaaaaatta
    11161 cggtacgaac gatttgagtt ccatcctcga aggcaatgcc ggcatcgacg ctgcctacga
    11221 tatgcgcggc gaaagcattt tcctgcgcgg ttttcaagcc gacgcatccg atatttaccg
    11281 cgacggcgtg cgcgaaagcg gacaagtgcg ccgcagtact gccaacatcg agcgcgtgga
    11341 aatcctgaaa ggcccgtctt ccgtgcttta cggccgcacc aacggcggcg gcgtcatcaa
    11401 catggtcagc aaatacgcca acttcaaaca aagccgcaac atcggtgcgg tttacggtta
    11461 gtgggcaaac cgcagcctga atatggacat taacgaagtg ctgaacaaaa acgtcgccat
    11521 ccgtctcacc ggcgaagtcg ggcgcgccaa ttcgttccgc agcggcatag acagcaaaaa
    11581 tgtcatggtt tcgcccagca ttaccgtcaa actcgacaac ggcttgaagt ggacggggca
    11641 atacacctac gacaatgtgg agcgcacgcc cgaccgcagt ccgaccaagt ccgtgtacga
    11701 ccgcttcgga ctgccttacc gcatggggtt cgcccaccgg aacgattttg tcaaagacaa
    11761 gctgcaagtt tggcgttccg accttgaata cgccttcaac gacaaatggc gtgcccaatg
    11821 gcagctcgcc caccgcacgg cggcgcagga ttttgatcat ttctatgcag gcagcgaaaa
    11881 tggcaactta atcaaacgta actacgcctg gcagcagact gacaacaaaa ccctgtcgtc
    11941 caatttcacg ctaaacggcg actacaccat cggccgtttt gaaaaccact tgaccgtagg
    12001 catggattac agccgcgaac accgcaaccc gaccttaggt tacaaccgcg ccttttccgc
    12061 ctccatcaac ccctacgacc gcgcaagctg gccggcttcg ggcagattgc agcctattct
    12121 gacccaaaac cgccacaaag ccgactccta cggcatcttt gtgcaaaaca tcttctccgc
    12181 cacgcccgat ttgaaattcg tcctcggcgg ccgttacgac aaatacacct ttaattccga
    12241 aaacaaactc accggcagca gccgccaata cagcggacac tcgttcagcc ccaacatcgg
    12301 cgcagtgtgg aacatcaatc ccgtccacac actttacgcc tcgtataaca aaggcttcgc
    12361 gccttatggc ggacgcggcg gctatttgag catcgatacg ttgtcttccg ccgtgttcaa
    12421 cgccgacccc gagtacaccc gccaatacga aaccggcgtg aaaagcagtt ggctggacga
    12481 ccgcctcagc actacgttgt ctgcctacca aatcgaacgc ttcaatatcc gctaccgccc
    12541 cgatccaaaa aacaaccctt atatttatgc ggttagcggc aaacaccgtt cgcgcggcgt
    12601 ggaattgtcc gccatcgggc aaatcatccc taaaaaactc tatctgcgcg gttcgttggg
    12661 cgtgatgcag gcgaaagtcg ttgaagacaa agaaaatccc gaccgagtgg gcatccattt
    12721 gaataacacc agcaacgtta ccggcaacct gtttttccgt tataccccga ccgaaaacct
    12781 ctacggcgaa atcggcgtaa ccggtacagg caaacgctac ggttacgact caagaaataa
    12841 agaagtgact acgcttccag gctttgcccg agttgatgcc atgcttggct ggaaccataa
    12901 aaatgttaac gttacctttg ccgcagccaa tctgttcaat caaaaatatt ggcgttcgga
    12961 ctctatgccg ggtaatccgc gcggctatac tgcccgggta aattaccgtt tctgatgaaa
    13021 tcaggcaaag gccgtctgaa aatcctgata atccgtaaat tcagcatacg aactttttga
    13081 caggctttat ctacatcagt ataatccaaa aagaaatagt gcccttggca gtacgcatta
    13141 cccattaaat catagcgggt atgccgatcc caagggcttt tttatatctt tgacaaatat
    13201 aggggaagca atatgaaaac cgccattttg attgacggtg cattttttat taagcgggta
    13261 cggtcattag gcctgtctca aaatccacac gatgcaaaaa gaattgctga tttggctttc
    13321 cgatattcga tgctgcatct gaaagataaa atcaacggca cagatcaaat ggatgattta
    13381 tatagaattt tcttttacga ctgcgtgccg tttgaaaaga aaatgcacaa tcccgtatct
    13441 catcgtgcca tagatttttc aaagactccg gaagccatat ttcgttgcaa tctgcatacc
    13501 gaattgaaga agaagcgtaa attagcgtta cgtttaggca agctgtcgga caatacagca
    13561 tggatattaa aaccccaagt catgaaaaat cttctgaaaa acccgtcaac tcaaattacg
    13621 gaaaacgatg tcgtgctcga tgttaaacaa aaaggtgtag atatgcgtat aggcttggat
    13681 atttcatcta ttaccttaaa aaaacaagcc gataaaatca tcttgttttc tggtgattcc
    13741 gattttgtcc cagcagccaa attagccaga cgggaaggta tcgattttat tcttgatcct
    13801 atgtggaatc atgtcccacc tgacttaatg gaacacatcg acggattgaa gacaaaatgt
    13861 ccgaaaccgt aaattactag aacaaaggcc gtctgaaaat aaggctaatt atctaagcca
    13921 tatttcagac ggcctttata aaggctgaaa taaaactaaa cacatttttt cactcaaatc
    13981 gaacacgcct tcaataaaat gtcataaaat ccgcacatta atctgacaca caagagatac
    14041 ctatgaaact gaaaacctta gctttgactt cattgaccct gttggcattg gccgcttgta
    14101 gcaaacaggc tgaaaccagc gttccggcag acagcgtcca aagcagctca tctgctccgg
    14161 cagccccagc cccattgacc gaaggcgtga actacactgt attgtccacg cctatcccgc
    14221 aacagcaggc cggcaaagtc gaagtcttgg aatttttcgg ctacttctgc ccgcattgcg
    14281 cccatcttga gccggtcttg agcgagcaca tcaaaacgtt taaagacgat acctatatgc
    14341 gccgggagca tgtcgtgtgg ggtgatgaaa tgaaaccttt ggcacgtttg gcggccgcag
    14401 tggaaatggc cggtgaatca gataaagcca acagccatat tttcgatgcg atggttaatc
    14461 aaaaaatcaa tctggccgat accgataccc tgaaaaaatg gctgtccgag caaacagcgt
    14521 ttgacggcaa aaaagtattg gctgcatttg aggcttctga aagccaagcg cgtgcggctc
    14581 aaatggaaga gttgaccaat aaattccaaa tcagcggcac accgactgtg atcgtcggcg
    14641 gcaaatacca agttgaattt aaagactggc agtccggtat gaccacgatt gaccagttgg
    14701 tggataaagt acgcgaagag cagaaaaagc cgcaataagt tgaggattga atgagtaaag
    14761 gccgtttgaa aatag
//
LOCUS       DAXHPG010000036        13994 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXHPG010000036 DAXHPG010000000
VERSION     DAXHPG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13994
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.036"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(103..1428)
                     /locus_tag="ACFM6F_000805"
     CDS             complement(103..1428)
                     /locus_tag="ACFM6F_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC6164263.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1643..2482
                     /gene="efeU"
                     /locus_tag="ACFM6F_000806"
     CDS             1643..2482
                     /gene="efeU"
                     /locus_tag="ACFM6F_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFC6164264.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            2479..3645
                     /gene="efeO"
                     /locus_tag="ACFM6F_000807"
     CDS             2479..3645
                     /gene="efeO"
                     /locus_tag="ACFM6F_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC6164265.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3909..5174
                     /gene="efeB"
                     /locus_tag="ACFM6F_000808"
     CDS             3909..5174
                     /gene="efeB"
                     /locus_tag="ACFM6F_000808"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC6164266.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPVHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5244..5573)
                     /locus_tag="ACFM6F_000809"
     CDS             complement(5244..5573)
                     /locus_tag="ACFM6F_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC6164267.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAVLQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNISCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5648..7018)
                     /gene="glmU"
                     /locus_tag="ACFM6F_000810"
     CDS             complement(5648..7018)
                     /gene="glmU"
                     /locus_tag="ACFM6F_000810"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC6164268.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLKTLLEAAGNKVGLLTDVPADPTGLGRIIRDSNGSVTAIVEEKDADAV
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKLQLTELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGDIELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     AKLADDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVNKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(7073..7345)
                     /locus_tag="ACFM6F_000811"
     CDS             complement(7073..7345)
                     /locus_tag="ACFM6F_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC6164269.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7321..8052)
                     /locus_tag="ACFM6F_000812"
     CDS             complement(7321..8052)
                     /locus_tag="ACFM6F_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC6164270.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFHLINRAMTRYMAHRLKLSES
                     AASDLRQEYWHRYGATLAGLQIHHPEIDIADFLRESHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTPPRKYERMLCVKPSLS"
     gene            8111..9112
                     /locus_tag="ACFM6F_000813"
     CDS             8111..9112
                     /locus_tag="ACFM6F_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6164271.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9206..10054
                     /locus_tag="ACFM6F_000814"
     CDS             9206..10054
                     /locus_tag="ACFM6F_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC6164272.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10080..10565
                     /locus_tag="ACFM6F_000815"
     CDS             10080..10565
                     /locus_tag="ACFM6F_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC6164273.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10667..12235)
                     /gene="msrAB"
                     /locus_tag="ACFM6F_000816"
     CDS             complement(10667..12235)
                     /gene="msrAB"
                     /locus_tag="ACFM6F_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC6164274.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALISKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12380..13645
                     /gene="ftsY"
                     /locus_tag="ACFM6F_000817"
     CDS             12380..13645
                     /gene="ftsY"
                     /locus_tag="ACFM6F_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC6164275.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKEAVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     IPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         3006 a         3569 c         4202 g         3217 t
ORIGIN      
        1 cgtcaaataa gaaaggaatt ttgtgcctgc ggtatcgcaa aacttcgcct taatgcgccc
       61 gattgactag ggatgggctt cagatggcat tgttttgccg gtttacgggc ggtattcggg
      121 cttcataccg ttgggtagga gctgccagac gtaacccgtg gttttctgtt tgccggcaag
      181 ttcgccggct tcgtcgccgt atccccaaaa ataatccacg cgcaccgcgc ctttaatcgc
      241 gctgccggta tcctgcgcca taatcaggcg gttgagggct ttgcgggtaa ccggatgggc
      301 ggtggcgaca aataagggcg cgcccaaggt aatgtagtgc cggtcgactg cgccggcata
      361 ttcccccatc aacggcgtgc ccagtgcgcc gacgggaccg tcattgctgc ttccggcaag
      421 ctcgcggaaa aagatatagc tggggttttg acccaaaact tcggcgaggc gttgcggatt
      481 ttgccgcata taggctttga tgccctgcat ggaggtctgc ccgagcttga ggtagccttt
      541 gtccgccata tagcgtccga tggaaacgta gggatgttcg tttttgtcgg catagccgat
      601 gcggatgtat ttgccggacg gggttttcag acggcccgag ccttggatgt gcataaaaaa
      661 aagttcgaca gggtcttcgg cgtaaccgag tatcggtgct ttgccgtcaa gcgcgccgcc
      721 gttgatttgg ttgcgcgtgt ggtaggggag gaagcggctt ccttcaaacc tgcctttgat
      781 tgccgttgtg cgcgcagtga tggggaattg ggagaggtcg gcggtatgtg tgccgccggt
      841 attgtcgatt gtgccgctgt tttttcccgt ctgcctgatg cggacaaggg cttttccgct
      901 ccgcaaaccg gcaggcaggg ggacggagat aaaatcgtcg ggaataccgt aaatcgggaa
      961 gcgggcttgt gccgtccgcc tgtcgtcgcc cttcagcacc ggctcgtaat agccggtaac
     1021 cgtaccggca aggcttccgt tgcctgcaac ctgccacggc gtgaaatagc gttcaaaaaa
     1081 ctgttttgcc tgaaaggaat ggacgggggt ttgaaaggct tgggcgcaca catcctgcca
     1141 gccttggcgg tttttcaaat tggcgcagcc gaggcggaag gattgcaggc ttttggcgaa
     1201 atcctgcgcc gcccagtggg gcagggacag gtgcggcaca acggtataaa cggccccgcc
     1261 gccgccgacc gtcgttccgg cggggtcggg gatgccgacc ggccggtccg ggccgttgat
     1321 gacggatgtg tcgggttgcg gaaaggtttg gatgctcttg ctttggcagg cggcgaggat
     1381 ggcggcggcg atgccgcaca gggcggcgcg gaacaggtat tttttcataa tggacaatgt
     1441 tgccggcagt aataaaaaaa aaagatggtt tcgggcggcg ttgcggcagc cgtggagagg
     1501 ggattttaac acagggcgca gctgcagcct gcggaacttt ccgccgcgcg gcaatgcaga
     1561 taaaaataac ttgcatttgt atttacaagc aatgaaaata ttccgataat atattattca
     1621 tcatccttgt tcgttcgcgt ttatgctggt cgctttttta attatgttgc gcgagggtat
     1681 cgaagccgcg ctcatcgtcg gcatcgttgc cggttttctg aaacagtccg gacattccaa
     1741 actgatgcct aaggtctggt tcggggtcgt ccttgcttct ttgatgtgtt tgggcatcgg
     1801 atacggcatc cattcggcaa cgggcgagat tccccagaag cagcaggagt tcgtcgtcgg
     1861 cattatcggt ttggttgctg tcgcgatgct gacttatatg attttatgga tgaaaaaggc
     1921 ggcgcgttcg atgaagcggc agcttcagga ttctgtacag gcggctttga accgtggcag
     1981 cggtcaagga tgggccttgg tcggtatggc gtttcttgcc gtggcgcgcg aaggtttgga
     2041 gagcgttttt ttcctgcttg ccgtattcaa acagagcccg acgtggcaga tgccggccgg
     2101 tgcggtagcg ggggttttgg ctgccgccgt gattggcgcg ttgatttatc agggtgggat
     2161 gcgcctgaat ctggctaagt ttttccgttg gacgggggcg tttttgattg tcgttgccgc
     2221 cggcctgctt gccggctcgc tgcgcgcgct gcacgaagca ggcatttgga acgcgcttca
     2281 ggacattgtg ttcgactcat caaaatattt gcacgaagac agtccgttag gtgtgctgct
     2341 cggcggattt ttcggctata ccgaccatcc gacgcagggc gaggccttgg tttggctgct
     2401 gtaccttatt cccgtcatga cttggttttt gtgcggcagc aggccgtctg aaactttaac
     2461 ccgtaaagag gagctgaaat gagaaaattc aatttgaccg cattgtccgt gatgcttgcc
     2521 ttaggtttga ccgcgtgcca gccgccggag gcggagaaag ctgcgccggc agcgtccggt
     2581 gaggcgcaaa ccgccaacga gggcggttcg gtcagtatcg ccgtcaacga caatgcctgc
     2641 gaaccgatgg aactgaccgt gccgagcgga caggttgtgt tcaatattaa aaacaacagc
     2701 ggccgcaagc tcgaatggga aatcctgaaa ggcgtgatgg tggtggacga gcgcgaaaac
     2761 atcgcccccg gactttccga taaaatgacc gtcaccctgt tgccgggcga atacgaaatg
     2821 acttgcggtc ttttgaccaa tccgcgcggc aagctggtgg taaccgacag cggctttaaa
     2881 gacaccgcca acgaagcgga tttggaaaaa ctgtcccaac cgctcgccga ctataaagcc
     2941 tacgttcaag gcgaggttaa agagctggtg gcgaaaacca aaacctttac cgaagccgtc
     3001 aaagcaggcg acattgaaaa ggcgaaatcc ctgtttgccg acacccgcgt ccattacgaa
     3061 cgcatcgaac cgattgccga gcttttcagc gaactcgacc ccgtcatcga tgcgcgtgaa
     3121 gacgacttca aagacggcgc gaaagatgcc ggatttaccg gctttcaccg tatcgaatac
     3181 gccctttggg tggaaaaaga cgtgtccggc gtgaaggaaa ttgcagcgaa actgatgacc
     3241 gatgtcgaag ccctgcaaaa agaaatcgac gcattggcgt ttcctccggg caaggtggtc
     3301 ggcggcgcat ccgaactgat tgaagaagtg gcgggcagta aaatcagcgg cgaagaagac
     3361 cggtacagcc acaccgattt gagcgatttc caagccaatg tggacggttc gaaaaaaatc
     3421 gtcgatttgt tccgtccgct gatcgaggct aaaaacaaag ccttgttgga aaaaaccgat
     3481 accaacttca aacaggtcaa cgaaattctg gcgaaatacc ggactaaaga cggttttgaa
     3541 acctacgaca agctgggcga agccgaccgc aaagcgttac aggcctctat taacgcgctt
     3601 gccgaagacc ttgcccaact tcgcggcata ctcggcttga aataagccgc aagcgttcag
     3661 acggtatttg gcggcagata ccgtctgaag ttttatagtg gattaacaaa aaccagtacg
     3721 gcattgcctc gccttgcctt gccgtactat ttatactgtc tgcggcttcg tcgccttgtc
     3781 ctgatttttg ttaatccact atatccgcca tatattgcag ggcaggattt caacctgccg
     3841 ctatcggtta atggaaaaac ggcgtgcagg gatacccatc ctgctgcacg gatattgaag
     3901 gaaacaccat gagcaaaaac caacccgcac aaccgaccag gcgcactctt tttaaaaccg
     3961 cgatcgcagc cggagcagtc ggcgcaatcg gaggttatct cggcggcaaa aaacagggcg
     4021 aaaccgccga acgcaccgcc gaaagccaac actcgcccca agcctatccc tgctacggcg
     4081 aacatcaggc aggcatcgtt acgccgcagc aggcgttttc gattatgtgc gccttcgacg
     4141 taaccgcgca aagtgccaag cagctggaaa acctgttccg cacgctgacc gcccgcatcg
     4201 agtttctcac ccaaggcggc gaataccaag acggcgacga caaacttcca cccgccggca
     4261 gcggcatttt gggcaaagcc ttcaaccccg acgggttgac cgttaccgtg ggggtgggca
     4321 gcagcctgtt tgacggccgg ttcggactca aagacaaaaa accggttcat ttgcaggaaa
     4381 tgcgcgactt ctccaacgat aagctgcaaa aaagctggtg cgacggcgat ttgagcctgc
     4441 aaatctgcgc cttcaccccc gaaacctgcc aagccgccct gcgcgacatc atcaaacaca
     4501 ccgtccaaac cgccgttatc cgttggagta tcgacgggtg gcagcctaaa tccgaacccg
     4561 gcgcgatggc ggcgcgcaac ctgttgggct tcagggacgg cacgggcaac cccaaagttt
     4621 ccgaccccaa aactgccgac gaggttttgt ggacgggtgt ggccgccaac agcctcgacg
     4681 aaccggagtg ggcgaaaaac ggcagctatc aggcagtccg ccttatccgc cactttgtcg
     4741 agttttggga caggacgccg cttcaagagc aaaccgacat tttcgggcgg cgcaaataca
     4801 gcggtgcgcc gatggacggc aaaaaagaag ccgaccaacc ggattttgcc aaagaccccg
     4861 aggggaatac cacgcccaaa gacagccata tacgcctggc gaatccgcgc gatcccgaat
     4921 tcctcaaaaa acaccgcctc ttccgccgcg cctacagcta ttcgcgcgga ctcgcctcaa
     4981 gcggacagct tgatgtcggg ctggtgttcg tctgctatca ggcaaacctt gccgacggat
     5041 tcatcttcgt gcaaaacctc ctcaacggcg aaccgctgga agaatacatc agccccttcg
     5101 gcggcggcta tttcttcgtg ctgcccggcg tggaaaaagg cggcttcttg ggacaagggc
     5161 tgctgggtgt ataaacctgc ctgacggaca aatgccgtct gaatccgata aggggttcag
     5221 acggcatttt agtgtttggg cggtcaggct tttttgacga attcagattt taaattcatc
     5281 gcgctgccgt cgattttgca ggagatgttg tgatcgcctt cctgtaggcg tatgcctttg
     5341 acttttgtac cttgtttgat cgtcatcgag ctgcctttta ccttgaggtc tttgatgagg
     5401 atgacggtgt cgccgttttg cagcactgcg ccgttggcat cgcgcacttg agccgcaagg
     5461 tcggcggcgg attcggtttc attccattcg tgggcgcatt cggggcagat gtattgtccg
     5521 ccgtcttcat aagtgtattc ggaggcgcat tgtgggcatg ggggtaatga catggtttgc
     5581 agtccttatc ggatgtttgt tttggggtac cgtctgaaac ctgaaaccgg cttcagacgg
     5641 catggcttta ttgcttgtct ttttcgggac gcacccagcc ttcgatgacg gtttggcggg
     5701 cgcgggcgag ggcgagtttg ttgtcttcga tattgcgggt aatcgtgctg cctgcgcctg
     5761 tggttacttt gttgcccagt gttacagggg caaccagtac gcagtttgaa ccgatgcgca
     5821 cttcgtcgcc gatgacggtt ttgtgtttgt tcacgccgtc gtagttggca ataatcgtac
     5881 ctgcgccgaa gttggttttg ctgccgactt cggcatcacc aatgtaggtg aggtggttgg
     5941 ctttggtgcc tttgccgatg gcggcgtttt tgatttcgac gaagttgccg acgtggacat
     6001 cgtcagccag tttggcttgc ggacgcaggc gggcgtacgg gccgattcgg ttgttttcgc
     6061 cgacttcgca gctttcgagg tgggagaagg gggcgatttt gctgtttgcg ccgattttgg
     6121 cgtttttgat gacgcagttt gcgccgattt cgacgttgtc gccgagctcg atgtcgcctt
     6181 caaagataca gttcacatca atcacgacgt cttgcccgtg tttcagacgg cctcgtaaat
     6241 cgaaacgtgc cggatcgcgc agggttacgc ctgctttgag caattcttgc gcctgttcgg
     6301 tttggaagat gcgttcgagt tcggtgagct ggagtttgtt gttcacgccg gcggcgaggt
     6361 gggaggcgcg cacttggacg ggatgaactt taataccgtc ggcaacggct ttggcgatga
     6421 ggtcggtcag gtagtattcg ccttgtgcat tgttgctgga aaggctgttc agccagtttt
     6481 cgagtttggc gttgggcagg acgaggatgc cggtattgat ttctttcacg gctttttgga
     6541 cggcgtcggc gtctttttct tcgacgatgg cggttacgct gccgttgctg tcgcggatga
     6601 tgcgccccaa gcctgtcggg tcggcgggca cgtcggtcaa cagcccgact ttgttgcctg
     6661 cggcttcgag cagggttttg agggtttcaa cgtcaattaa aggaacgtcg ccgtacaaca
     6721 ccagcgtgcg gccttcggcg gcaaggtggg gcagggcggt tttgacggcg tggccggtgc
     6781 cgagttgttc ggtttgttca acccagacga catcgcgttt gacggtgtcc aaaacttgct
     6841 ctttgccgtg tccgacgacg acgcagatgt tttgcggatt cagcgcggcg gcggtgtcga
     6901 taacgcgccc gagcatgggc ttgccgccga tgcggtgcag cactttgggc attttggaat
     6961 acatgcgcgt gcctttgccg gcggcgagga tgacgatgtt taaagtgttt tgcggcatag
     7021 cgttttcctg tgcaatgccg tctgaaaggg cttcagacgg catagggtgg gtttatcggt
     7081 tttgaaattt gggcttttgc cagtgttggc gatgctcttc gtcggcgttg ttgccggttt
     7141 ggttgggtaa cacggcatgg cgttcgggac ggtattggtt gtagttcata tttttcgagt
     7201 agctgccgtc ttggtaataa acgggcgtgc cggcgggata tttttgacgg acggcggtct
     7261 tgccgttgcc gtcttgataa gtttcccacg cgcagcccga caaaagggcg gcggcagtca
     7321 tcaggataag gaaggtttta cgcataacat tctttcgtat tttcgggggg gtgtgcgggt
     7381 attgtaatga ttttggcggt gttctgacaa agtttctgca taccgagcca gttgcgccat
     7441 atcgcttacg gaagcatcga taaagggcag gtcgtgcact tttgcaccga accggacggt
     7501 tttcatgccc aatgcttttg cctgatgcag gttgtccgcg ctgtcgtcca ccataatgca
     7561 gcattcgggc ggtacgtcca aaaggcggca gacattgaga tacgcttgcg gattgggttt
     7621 gtacagcagt ccgaaatcat ccgtgccgaa cagtgcgtcg aaacggtttt ccaaaccgag
     7681 tgcgccgaca acggcgcgga cgtaaaacga cgggccgttg gaaaaaactg ccttgcgccc
     7741 ttttaggcgg ctcagggtgt tttgtgtttc aggcatgccg tgcagcctag tcaggattgc
     7801 atcgatcgga tggctttcgc gcaaaaaatc ggcgatgtcg atttcgggat ggtggatttg
     7861 cagtccggca agcgttgcgc cgtagcggtg ccaatattcc tgacgcaggt cggacgcggc
     7921 ggattcggag agtttgaggc ggtgtgccat atagcgtgtc atagcgcggt tgatgaggtg
     7981 gaagatgccg gcatcggcat tgtgcagcgt gttgtcgagg tcgaacagcc acacggtcgg
     8041 gttttcttgc atgttgaacc gtgaaaattt gttagaatgt tattttacag cgaatagagg
     8101 aggactcgga atgaaacgga aaatttggct gctgccgctg ctggcggttt cggcatacct
     8161 gcaggcgcag acggaagtca ggctggcggt gcataagtcg ttcagcctgc ccaaagggtt
     8221 gattgcgcgc ttcgagcggg caaacgatgc gaaggtgtcg attattcagg cgggcggcgc
     8281 gaacgaaatg ctcaacaaac tgattttgag ccgcgccaac ccgattgccg acgcggtgta
     8341 tggtttggac aacgccaata tcggcaaggc gcgggaaatg ggcattttgg cggcggcgca
     8401 acccgaatcc gcccccgtcg cggtcgggct gccttcggct ttggcggtcg attacggcta
     8461 tgtgtccatc aattacgaca aaaaatggtt tgaaggcaaa aagctgcccc tgccgcaaac
     8521 cctgcaggat ttgacccgcc ccgaatataa aaacctattg gtcgtgccgt cccccgccac
     8581 gtcgtccccg gggctgggct tcctgatggc gaacatcagc ggtctgggcg aagaaggcgc
     8641 gttcaaatgg tgggcacaga tgcggcagaa cggcgtgaag gtcgccaaag gctggagcga
     8701 ggcgtattac accgactttt cgcacaacgg cggcgcgtat ccgctggtgg tcggttatgc
     8761 cgccagcccg gcggcggaag tgtatttttc caaaggcaaa tacagcgagc cgccgacggg
     8821 caacctgttt ttaaaaggcg gcgtattccg ccaggtcgaa ggcgcggcgg tcttgaaggg
     8881 cgcgaaacag ccggaattgg cggcaaaact ggtgcaatgg ctgcaaagtc gggaagtgca
     8941 gcaggcggtt ccgtccgaaa tgtgggttta ccccgccgtc aaaaacacgc gcctgcccga
     9001 cgtgttccgc ttcgcccaag ccccgacgca caccaccgcc cccgcgcagc gcgatattga
     9061 tgcgaaccag cgcggatggg tttcccgttg gattagaacg gttttgaaat aaaacaaaca
     9121 taccgccccg cagggcttca tacggcattt ttacacctgt gccgattacg ccgcacgggg
     9181 cggatgttcg atcaagagga aaacaatgga cttcaaacaa tttgattttt tacacctgat
     9241 cagtgtttcc ggttgggagc atctggctga aaaggcgtgg gcgttcgggc tgaaccttgc
     9301 cgccgcgctg cttatttttt tggtcggaaa atgggcggcg aaacgcattg tcgccgtgat
     9361 gagggcggcg atgacgcgcg cgcaggtcga tgccacgctg attagttttt tgtgtaatgt
     9421 tgccaatatc ggcttattga ttttggtgat tattgccgca ttgggcagat tgggcgtttc
     9481 cacaacatcc gtaaccgcct taatcggcgg cgcgggtttg gcggtggcgt tgtccctgaa
     9541 agaccagctg tccaattttg ccgccggcgc gctgattatc ctgttccgcc cgttcaaagt
     9601 cggcgatttt atccgcgtcg gcggttttga aggatatgtc cgagagatta aaatggtgca
     9661 gacttctttg cggacgaccg acaacgaaga agtcgtgctg cccaacagcg tggtgatggg
     9721 caacagcatc gtcaaccgtt ccacactgcc gctgtgccgc gcccaagtga tagtcggcgt
     9781 cgattacaac tgcgatttga aagtggcgaa agaggcggtg ttgaaagccg ccgtcgaaca
     9841 ccccttgagc gttcaaaacg aagagcggca ggccgccgcc tacatcaccg ccttgggcga
     9901 caatgccatc gaaatcacat tatgggcttg ggcaaacgaa gcagaccgct ggacgctgca
     9961 atgcgacttg aacgaacaag tggtcgaaaa cctccgcaaa gtcaatatca acatcccgtt
    10021 cccgcaacgc gacatacaca tcatcaattc ttaaacgccg tctgaaagag gagtgggaaa
    10081 tggacgcgct gcacaccatc gcccgaaacc tgacgaaaaa acgtcaaacc gtaagctgtg
    10141 ccgaatcctg cacgggtggg ctgcttgccg ccgtattcac aagcgttgca ggcagttcgc
    10201 aatggttcga tcaaagtttt gtaacataca gcaacaaagc caaagaagac cgcttgggcg
    10261 tgttgcccga aaccctgctc gaacacggcg cggtcagccg ccaaaccgtc tatgagatgg
    10321 cgcgcggcgc gaaagccgtg gcgcaggcgg attacgccgt cggtatttcc ggcatcgccg
    10381 gtccgggcgg cggcagcgaa agcaaacccg tcggcacggt ttggttcggg tttgcctttc
    10441 cgggcggaag ttgcgaagca atgcgccgtt ttgacggcaa ccgcgaatcc gtccgcgcgc
    10501 aggcggtcgc cttcgcgttg gaacggttgg cggggctgat tgaaaacggc ggcgatgctg
    10561 tctaaacaaa atctccgtct gaacaaaatc cccatcggat aaaaaatgcc gtctgaaacg
    10621 tttcgggttt cagacggcat tttgtcgggt taggcggcgg tgcggcttat ttcactttac
    10681 tcttcaacgc gccgtagcct gccgcgtcca tttgttccag cgggatgaat ttcaagctcg
    10741 cgccgttgat gcagtagcgc agtccgcctt tgtcgcgcgg gccgtcgggg aagacgtgtc
    10801 ccaaatgcga atcggcggcg tggctgcgca cttcggtgcg gcgcatgttg tagctgaaat
    10861 catcgtgttc ggtaacggat tttgcatcaa tcgggcgcgt gaagctcggc cagccgcagc
    10921 cggaatcata tttgtcggcg gagctgaaca aaggttcgcc gctgacaacg tccacataaa
    10981 tgccgggttt gaacaaatgg tcgtattcgt ggctgaaggc atattcggtc gcgctgtttt
    11041 gggtaacttg gtattgctct tcggtcaggg tgcgtttgag ttcggcgtca ctcggttttt
    11101 tatacgttgc cgcatcgaag cctttgcctt gcggggcggc tttggttttg cccggcagcg
    11161 gttcgtcggc tttgcggata tcgatgtggc agtagccgtt ggggttttta atcaggtagt
    11221 cctgatggta ttcctcggca tcgtagaagt ttttcagcgg ctcgttttca acaacgaggg
    11281 gcagttggta tttttgctgc tcgcgtttga gggcggcggc gatgacggct ttttcggcgg
    11341 ggtcggtgta gtacacgccg ctgcggtatt gcgtaccggt gtcgttgccc tgtttgttga
    11401 ggctggtcgg atcaacgacg cggaagaaat attgcaggat gtcgtctagg ctgagtttgt
    11461 cggcatcgta ggtcacttta acggtttcgg cgtggcccgt atggcggtag gacacgtctt
    11521 catagctcgg atttttcgtg ttgccgttgg cgtagccgga taccgcgtca accacgccgt
    11581 cgatgcgttg gaaataggct tccaagcccc agaagcagcc gccggcgagg taaatggtgc
    11641 gcgtgttcat gatttttgaa tcctttttct gagtgtcggg tttgtagaac gaatgtttca
    11701 agctgcccaa atcggcattc gggtcgcgga ttaacgccaa cgcctgcgct tcgttgatgc
    11761 tgcctttgac gatgcgctgc acgtcgccgt ctttactgat taacgcccac gaagggtaaa
    11821 cgctgatatt caggctttgg gcgatcgtgc cgccgttgtc ggttacgacg ggcagcttgg
    11881 gataattcaa accggcatac catttttgga agtcgccgtc ttttttctcg tgcaaaaagc
    11941 ccggagacgc gacggtaatc aggttggcgg agctgaattt tgcatcttgc gcccattttt
    12001 cggtctgtcc caattcggac aaacacaaag gacaccagct cgcccaaaat ttaatcagcg
    12061 tcggtttgtc ttttttcaag taaacactgg cggggcggtt gtccgcagtt ttcaaagtgg
    12121 ataaagtgtg cggcacggtc gcggctccgg catcgacgat tttgggcgaa caagcaccca
    12181 gcgcaagcag gcagccgaac ttggcgcaaa gggaaaagaa agtacggtgt ttcattttga
    12241 gtttcctgtg tggacggttt gcatgattag acgtttgaga tgccgaaacc ttacagctcg
    12301 gattttcaga caaccttgcc gcgtaaaata cgctacaata cgccctgttt caagtttcta
    12361 aaattaaaag gaaaattcaa tgttcagctt cttccgtcgc aagaaaaaac aggaaacgcc
    12421 ggctctcgag gaggctcaaa ttcaggaaac cgcagcaaaa gcagagtccg aaaccgatca
    12481 agtaattgaa aatattaaag aagatgctga atctttagca gaaagcgtca aagggcaggt
    12541 cgaatctgcc gttgaaaccg tcagcggtgc ggttgaacaa gtaaaggaag ccgttgccga
    12601 gatgctgtct gaagcagagg aagcggcgga aaaagcagcg gaacaagtcg aagcggcaaa
    12661 agaagccatt gccgaaaccg tcggcgaggc tgtcgggcaa gttcaagaag ccgttgcaac
    12721 aactgaagaa cacaagctcg gttgggcggc gcgtttgaaa caaggtttga ccaaatcgcg
    12781 cgacaaaatg gcgaaatcgc tggcgggcgt gttcggcggc ggacaaatcg acgaagattt
    12841 gtacgaagag ctggaaaccg tgctgattac cagcgacatg ggcatggaag ccaccgaata
    12901 cctgatgaaa gacgtgcgcg accgtgtcag cctcaaaggg ctgaaagacg gcaacgaatt
    12961 gcgcggcgcg ttgaaagaag ccttgtacga cctgattaag cctctggaga aacctttggt
    13021 tttgcccgaa accaaagagc cgtttgtcat catgcttgcc ggcatcaacg gcgcgggcaa
    13081 aaccacgtct atcggcaaac tcgccaaata tttccaagcg cagggcaaat ccgtattgct
    13141 ggcggcaggc gatactttcc gcgccgccgc gcgtgagcag cttcaagctt ggggcgaacg
    13201 caacaacgta accgtgattt cgcaaaccac gggcgattcc gccgccgtgt gcttcgatgc
    13261 cgtccaagcc gccaaagcgc gcggcatcga cattgtgctt gccgacaccg ccggccgcct
    13321 gcccacgcag cttcatttga tggaagaaat caaaaaagtg aaacgcgtat tgcaaaaagc
    13381 catacccgac gcgccgcacg aaatcatcgt cgtgctcgat gccaatatcg ggcaaaacgc
    13441 tgtcaaccaa gtcaaagcct ttgacgacgc attggggctg accggcttaa tcgttaccaa
    13501 actcgacggc acggcaaaag gcggcatcct cgccgcgctt gcctccgacc gccccgtccc
    13561 cgtccgctat atcggcgtgg gcgaaggcat agacgacctg cgcccgtttg acgcgcgcgc
    13621 gtttgtggac gcactgctgg attgagccga aatgccgtcc gaaaacagca gaccgatgcc
    13681 gtcattcccg cgcaggcggg aatccagacc ttgggataac ggcaatattc aaaggttatc
    13741 tgaaagtccg agattctgga ttcccacttt cgtgggaatg acgggatgta ggttcgtggg
    13801 aatgacgtgg tgcaggtttc cgtatggatg gattcgtcat tcccgcgcag gcgggaatct
    13861 agaacgtaaa atctaaagaa accgtgttgt aacggcagac cgatgccgtc attcccgcgc
    13921 aggcgggaat ctagaccatt ggacagcggc aatattcaaa gattatctga aagtccgaga
    13981 ttctggattc ccac
//
LOCUS       DAXHPG010000037        13481 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXHPG010000037 DAXHPG010000000
VERSION     DAXHPG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13481
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.037"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(144..234)
                     /locus_tag="ACFM6F_000818"
     tRNA            complement(144..234)
                     /locus_tag="ACFM6F_000818"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ser,seq:gga)
     gene            complement(273..359)
                     /locus_tag="ACFM6F_000819"
     tRNA            complement(273..359)
                     /locus_tag="ACFM6F_000819"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Leu,seq:cag)
     gene            complement(395..481)
                     /locus_tag="ACFM6F_000820"
     tRNA            complement(395..481)
                     /locus_tag="ACFM6F_000820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Leu,seq:cag)
     gene            570..2945
                     /gene="rnr"
                     /locus_tag="ACFM6F_000821"
     CDS             570..2945
                     /gene="rnr"
                     /locus_tag="ACFM6F_000821"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC6164276.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIEALVRELSIKEEEYEFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQL
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3284..4747)
                     /gene="guaB"
                     /locus_tag="ACFM6F_000822"
     CDS             complement(3284..4747)
                     /gene="guaB"
                     /locus_tag="ACFM6F_000822"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC6164277.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4948..5028)
                     /locus_tag="ACFM6F_000823"
                     /pseudo
     CDS             complement(4948..5028)
                     /locus_tag="ACFM6F_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(5074..5535)
                     /locus_tag="ACFM6F_000824"
     CDS             complement(5074..5535)
                     /locus_tag="ACFM6F_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164278.1"
                     /translation="MNIIMDSTRKFGILWEENSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNAVFNSLKSSFEEKYYAGGNNGLDFGEQLFDIDKYNSLELCNIFSIDTTYMSG
                     GGNCEIDCLVLEMGYSGEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(5626..8184)
                     /gene="glnD"
                     /locus_tag="ACFM6F_000825"
     CDS             complement(5626..8184)
                     /gene="glnD"
                     /locus_tag="ACFM6F_000825"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC6164279.1"
                     /translation="MPANLSSALETFKRQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTVDKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNPENRRRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIIGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSLDLKNPKIQSSLKQTLLEQLS"
     gene            complement(8232..8552)
                     /locus_tag="ACFM6F_000826"
     CDS             complement(8232..8552)
                     /locus_tag="ACFM6F_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6164280.1"
                     /translation="MMGNKLTPPAKLPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(8877..9350)
                     /gene="bfr"
                     /locus_tag="ACFM6F_000827"
     CDS             complement(8877..9350)
                     /gene="bfr"
                     /locus_tag="ACFM6F_000827"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6164281.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(9378..9842)
                     /gene="bfr"
                     /locus_tag="ACFM6F_000828"
     CDS             complement(9378..9842)
                     /gene="bfr"
                     /locus_tag="ACFM6F_000828"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6164282.1"
                     /translation="MQGNQAVLDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(10305..11288)
                     /gene="lipA"
                     /locus_tag="ACFM6F_000829"
     CDS             complement(10305..11288)
                     /gene="lipA"
                     /locus_tag="ACFM6F_000829"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC6164283.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYKKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQATEALRESHGGCGHH"
     gene            complement(11281..11904)
                     /gene="lipB"
                     /locus_tag="ACFM6F_000830"
     CDS             complement(11281..11904)
                     /gene="lipB"
                     /locus_tag="ACFM6F_000830"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC6164284.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11906..12181)
                     /locus_tag="ACFM6F_000831"
     CDS             complement(11906..12181)
                     /locus_tag="ACFM6F_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC6164285.1"
                     /translation="MTEQENKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            12366..13436
                     /locus_tag="ACFM6F_000832"
     CDS             12366..13436
                     /locus_tag="ACFM6F_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC6164286.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLFALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
BASE COUNT         2944 a         3185 c         3808 g         3544 t
ORIGIN      
        1 ataccacaaa acataccata aaaaaacggt aacggatagt tgtaagcggt tgatgacgat
       61 tatagacaat aacgggtttt cccaatgaaa ttattgtttg aataataaaa aatcccaaac
      121 cgtaaaagtt tgggatttgt atttggcaga gaggaaggga ttcgaaccct cgatacgcta
      181 ttcacgtata cacgctttcc aggcgtgcga cttaaaccac tcatccacct ctctaatggc
      241 ggaaattatc ccaatcggga taatttatta tttggtgccc gggagaagac tcgaacttcc
      301 acacccgtga ggataccagc acctgaagct ggcgcgtcta ccaattccgc cacccgggca
      361 atctaaatat tgaaataaag caaagcattt gatttggtgc ccgggagaag actcgaactt
      421 ccacacccgt gaggatacta gcacctgaag ctagcgcgtc taccaattcc gccacccggg
      481 ctttgcttta tttgctgttt tgcggtacag tgtcctgccg caaagaagtc gctattatat
      541 agttcataaa gagaatgtca acagtccaaa tgaataaaaa tattaaatct ttaaatttac
      601 gggaaaaaga cccgttttta agtcgtgaaa aacagcgtta tgaacatcct ttgcccagtc
      661 gggaatggat aatcgagctg cttgaacgta aaggcgtacc atccaagatt gaagctttgg
      721 tacgcgaatt gtcgattaag gaagaagagt acgaattttt cgaacgtcgt ctgaaggcga
      781 tggcgcggga cggtcaggtt ttaatcaacc gtcggggcgc ggtttgcgcg gcggacaaat
      841 tggatttggt caaatgccgt gtcgaggcgc acaaagacgg cttcggtttc gccgtgccgc
      901 tcacgcccgc caaagacggt gattttgttt tgtacgaacg ccagatgcgc ggcattatgc
      961 acggcgatat tgtcactgtt cgtcctgccg gcatggaccg taggggccgc cgcgaaggga
     1021 cggttctgga tattgtcgaa cgcgcgcaaa gcaaagttgt cggccgtttc tatatggata
     1081 ggggcgtggc gattttggag ccggaagaca agcgtctgaa ccaaagcatc gtattggaac
     1141 cggacggcgt ggcgcgtttc aaacctgaat ccggtcaggt catcgtcggc gaaattgagg
     1201 tttatcctga gcaaaaccgg ccggcagtgg caaaaatcat cgaagttttg ggcgattatg
     1261 ccgacagcgg catggagatt gaaattgccg tgcgcaagca tcatttgccg caccaattca
     1321 gtgaagcgtg tgccaaagcc gcgaaaaaaa ttcccgacca tgtacgcaaa agcgatttga
     1381 aaggccgcgt cgatttgcgc gatttgcctt tggtaacgat agacggcgaa acggcgcgcg
     1441 attttgacga tgccgtgttt gccgaaaaaa tcggacgcaa ttaccgtctg gtcgtggcga
     1501 ttgccgatgt cagccattat gtccgccccg atgacgctat cgacacggac gctcaggaac
     1561 gcagcaccag tgtttacttc ccgcgccgcg tgattcccat gttgccggaa aacctgtcca
     1621 acggcatctg ctcgctcaat cctaatgtcg agcgtttgtg tatggtgtgc gatatggtca
     1681 tcacttacgc gggcaatatt aaagaatacc gcttctaccc cgccgtgatg cgctctcatg
     1741 cccgcctgac ctacaaccaa gtttggaaat ggctttcaga cggcatcggg catccgttca
     1801 aagcacaaat cgacacgctt tacaaactct tcaaaatcct tcagaaaaag cgtttcgaac
     1861 gcggggcggt ggagtttgac agcatcgaaa cccaaatgct tttcgacgac aacggtaaaa
     1921 ttgaaaaaat cgtccccgtt gtccgcaacg atgcccacaa gctgattgaa gaatgtatgt
     1981 tggcggcaaa cgtttgcgca gcggattttc tgttgaaaaa caagcatacc gcattgttcc
     2041 gcaaccattt ggggcccacg cccgaaaaac tcgccgcctt gcgcgagcag ctcggtctgt
     2101 tggggcttca acttggcggc ggcgacaacc cgtcgccgaa agactatgcc gcgcttgccg
     2161 gacagttcaa aggcaggccg gatgccgaat tgctgcaagt catgatgttg cgctccatgc
     2221 aacaggcggt ttacgaaccg cattgcgacg gacactttgg tcttgcctac gaagcatacg
     2281 cccacttcac ctcgcccatc cgccgctatc ccgacctgac cgtacaccgc gccatcaaag
     2341 ccgtgttgaa tcagcaaacc tacacgccaa aaaaaagctg gcaggctttg ggcgtgcata
     2401 cctcgttctg tgagcgccgt gccgacgacg ccagccgcga cgtggaaaac tggctgaaaa
     2461 cctattatat gcgcgataag gtcggcgaag tattcgaagg taaaatctcc ggcatgacca
     2521 gttttggtat ctttgtaaca ctggacggca tccacattga cggcttggtg catatcagcg
     2581 atttgggcga agactatttc aacttccgcc ccgaaatcat ggcaatcgaa ggcgaacgca
     2641 gcggcatccg tttcaacatg ggggacaggg ttgccgtccg ggtcgcccgt gccgatttgg
     2701 atgatggaaa aatcgatttt gtcctgattg ccgaggggag cggcaggggg cggaaagtta
     2761 aatcatccgc gtctgccaaa ccggcaggga cggcggggaa agggaaaccg aaaaccgccg
     2821 ccgagaaaaa aacagcccga ggcggcaaag taaggggaag gggcgcgtct gccgccgcag
     2881 aatcggggaa aaaggcaaag aaaccggttc cgattaaggt aaaaaaacgg aaaggcaaat
     2941 cataatgctg acggggtggc ttggggaggg cggggcatac tttcccgaag ccgccgcccg
     3001 attttttgat atggcggccg gcgcatcatt tcaggccaat cgaaacgccc gtattgaaag
     3061 attgcgttta tttccaccgc cgttttaaag gccggcggta ttcggcagac ggggcgcaaa
     3121 cggcgttcag acggcatttt cattctttcg gcgtgtccgt ccgaatggct ttgcccgtcc
     3181 gcgcaatcag cccgtgcggc ttgccttgaa cggacaaaaa atgccgtctg aaacccgaaa
     3241 atcaggtttc agacggcatt ttgccttgaa aaggctgttc aaatcagcgg tggtagttcg
     3301 gtgcttcttt agtgatttga acgtcgtgaa cgtgcgattc gctcatacct gcggaagtga
     3361 tttccacaaa ttcggctttt tcgtgcattt cggcaatatt ggcgcaaccc aagtaaccca
     3421 tactggaacg cagaccgccg gtcagctggt ggatgatgtt cacaatcggg cctttgtaag
     3481 gaacgcggcc ttcgatgcct tcggggacgt atttgtcggt gctgtcggtt ttgtcttgga
     3541 agtagcggtc ggcagaacct tggctcatcg cgcccaatga acccataccg cgataggatt
     3601 tgtacgagcg gccttgatag agttcgattt cgcccggcgc ttcttccgtg ccggcaaaca
     3661 taccgccgag catgacgctg tacgcgcctg cggcgagggc tttggcgatg tcgccggaga
     3721 agcggatgcc gccgtcggca atcaggggaa cgcctgtgcc tttgagggct tcggcaacgt
     3781 tgtggatggc ggtcagttgc ggcacgccca cgcctgccac gatacgggtg gtgcaaatcg
     3841 atcccgggcc gataccgact ttgacggcat ccgcaccggc ggcgaccaaa tccaatgcgg
     3901 ctttggcagt ggcgatgttg ccgccgatga cttggatgtg cggataggtt tctttcaccc
     3961 atttcacgcg gtcaatcacg ccttggctgt gcccgtgggc agtatcgacg acaatcacgt
     4021 ccacgccggc ttcgaccaag gctttgacgc gctcttcggt atcgccgccg gtgccgactg
     4081 ccgcaccgac gcgcagacgg ccttcggagt ctttgttggc gttgggaaac tcggtggttt
     4141 ttaaaatatc tttaacggta atcagacctt tgagttcgtc tttttcgttc agaaccaaaa
     4201 cgcgctcgac tttgtgcgtg tgcatcagtt cgcgcgcttc gtctatgctt gtgccttcgg
     4261 ggacggtaac caaacgttcg cgcggggtca taatggcgga aacgggcaaa tcgacgcggt
     4321 tttcaaaacg caggtcgcgg ttggttacga tgccgacgac tttgccgttt tcaacgacgg
     4381 gcaggccgga cattttgcgt ttgcgctgcg cacgcatttc caagacttcg cggatgagcg
     4441 tcgtcggtgc aacggttacg gggtctttga ccacgccgct ttcgtggcgt ttcactttgg
     4501 aaacggcgcg cgcctgcatt tcgggcggca tgtttttatg gatgatgccg atgccgcctt
     4561 cctgtgccat cgaaatggcg aggcgcgcct cggtaacagt gtccatcgca gcggaaagca
     4621 gggggaggtt gagtgtgatt tcgcgggtga gcttggtttg aagtttaacg tctcgcggca
     4681 gcacggtcga atgtgcggga accaacaaaa catcgtcgaa agtataggct ttttctacga
     4741 tacgcataat gctcggtctt tcagtttgtg caagatgcac ggcattatag cacgttaccg
     4801 gcggcttgac agtttatcag gtttaatttt ggtccccttt gaatagcccg gttttccttt
     4861 gccgaccact gttgctcccg ttctttcaat ttcaggaaaa gcttttttct aatttttggt
     4921 aagtggctca gttattgaag ccctatatcg ggcggtaact tccagcacaa aaaaacggag
     4981 tagttcttta tttatttttt cctttaattt tcagtatatt atcttaatat ttcgagggta
     5041 acatatctgc taatctagtt acagccccat atattataga ttcaattgaa aaataacaga
     5101 ttcaactgta cctttcttat acctgatttc tttaaagttt ttcccattgt caaaactata
     5161 aaaaagtctc tcttcctcac cagaatatcc catttctaat actaaacaat ctatttcaca
     5221 attaccccct ccactcatat atgtagtatc aatagaaaat atattgcata attccagaga
     5281 attatattta tctatatcaa atagttgctc tccaaaatct aaaccattgt ttccacctgc
     5341 ataatacttc tcttcaaagg atgacttcaa actattaaaa actgcattta gcgtaaaata
     5401 tccatatggg catatcttag gatatatatt ctctcctatt attatctgaa tttttccata
     5461 aataaaacca ttacattcag agttttcttc ccataaaatc ccaaattttc ttgttgagtc
     5521 cataataata ttcatataaa tccttatatt aataaattat ttacaatatc ccccgctttc
     5581 agacggcata cggcgtggcg gcggaatgcc gtctgaaggc gggcgttatg ataattgttc
     5641 cagcagcgtc tgcttcaatg aggactggat ttttgggttt ttcaggtcta ggctaaaaac
     5701 ggtaaaactg tcttcggcgc gttcatccag tgtggagatt ttggcatagc gcaggctgac
     5761 gttgtgggcg aaaaagactt ccgccatatc ggcgagcagg aaggggcggt tgacggcggt
     5821 gatttcgacg gagtaccaat cggggtagtc ttcttcgggg gtgatggtga tgcttggcgc
     5881 gatcggcata tagcggctgc ggcggctgat gcggcggcta tggctggaga tttcggcaac
     5941 ggtgtgtccg tggataaagc tgttgagttc ggcttcgagc gcgctttgaa tgtcggggta
     6001 gtcttcggga gcgtgctgcg aggggatttg cacgatgaag gtgtcgagga tgtagtcgtg
     6061 ttcggtgatg aaggcgcggg cggcgagaat gtcgaagccg tggcggctga agatgcggca
     6121 gaggcgggcg aacaggcgcg ggccgttggg cataaagacc atcacttgga agctgtcgct
     6181 ttggggcagg atgcggctgc ggacgatggg ggtttcaaaa tcgtgtacga ggttggcggc
     6241 gtgccacagg atttcgcggg actggtggcg ggcgaagtag gcggaaccga gcgcgttcca
     6301 tagttttttc tgctgttttt cggggacggc ggcgcgggtg agtaagtcgg cggcttcctg
     6361 ccggcggcgg ccgaagaggg tgtgcgggtt gccgccgttg cctgtaaggt agcgtccggc
     6421 ggcatggaag aggctttcca gcaggctggc gcgccatgcg ttccacagct tgggattggt
     6481 accgcgtatg tcggaaatgg tcagaaggta gagcgcgctg aggcgttcgt gggtttggac
     6541 gcgtttgcag aaagcatcga gtacgtcggg gtcttggatg tcttcttttt gggcgacggc
     6601 agacatcagc aggtggtttt cgaccagcca agcgagcagg tcgctttctt ctccggtcag
     6661 gaagtggtca gcggcaaatt ggcgcgcgtc tgcgatgcct tgtatggcat ggtcgccgcc
     6721 gcgtcctttg gcgatgtcat ggaaaaaggc ggcaaggtag aggatgtctt gtttttcaaa
     6781 ggactgcatc agtgcagagg cgtagggcag ctcgtggctg tgcatatcca aggcaaggcg
     6841 gcggacgttg cggacgacgg tgaggatgtg gtcgtccacg ggatagatgt ggaacaggtc
     6901 gtgttggagc aggccgatga ttttttccca cgcgggcagg tagcggccca acacgccgta
     6961 gaggttgaga aagcgcaggg tctgggtcag cccgttgccg ttgcggaaaa aaccggcgaa
     7021 gcggcggcgg ttttcgggat tttggtagaa gctgcggttg attttgcgcg tcgcgcccca
     7081 ccaggcgcgc agggtttgcg gttcgagcgc ggtaatgtcg ttgcgctgct gcatgatttc
     7141 gacgattttg aaaatgtgtt cgggccgtct gaaaaaaata tcggtgtgcc gcgcggcgat
     7201 ttggttgttg acttggatgt agtcgtcgtc aatccgcagg gtaacgcgca acggggtgga
     7261 ggaaacgcgg ctttgcagca taggcgtgag gatgccgccc agttgtttga cggttttaat
     7321 cgcgcggtaa aacacgcgca tcagttcttc gctttggcgg cggaggttca agccttcata
     7381 acccatgctt tcggcgactt gcggctgcaa atcgaacagc aggcggtctt cggcgcgatt
     7441 ggcgtttaaa tgcagatgga tgcggatgtg ggcgaggcgg cggtagccgt gcgaaagcat
     7501 accggcttcg gcacgcgtca aaatccgctg tttgagcagg tcgggcaggt cggtcgccaa
     7561 gccttgcgcc ttcgctatcc aaagcagggt gtggatgtcg cgcagaccgc ccggacagct
     7621 tttgatattc ggctccaata ctgcccccga accttgcgat ttggcgtggc ggtgttccat
     7681 ctccaccagt tttgcctcga caaacgccgc cacattgcgc tgcgcgttca ttttttccgc
     7741 cagtttatcc accgtttggc ggttgccaaa caaaaaccta gcctctaaaa acgctgtgtc
     7801 ccccgtaata tcattgcgca cgctttcaca tagttcatca acgctgccgc tttttacaga
     7861 cggcatcagt ttgcagtccc acagggtttg aacaaaccgg gcaatctgtt cctgaatgcc
     7921 gtctgaaagc ggggcagggg agacaaccgc caaatccaca tccgaacagg gatacagttc
     7981 gccgcgcccg aagccgccca ccgccatcag gcataacgcg ctgttttgaa aatgttctac
     8041 ccacaatgcc gccagtaagg tttcgactgc cgccgtatat tccctgaaaa ataccgacac
     8101 gcggttggct ttcaaataat gcgcttcggc ggcatcgcgc tgccgtttga aggtttccag
     8161 tgctgaagac aggtttgcag gcatttttat tctttcgatt ggcgggaaaa gggaggtgga
     8221 tggttcggcg gtcaaatacc gctttcagac ggcatttgtc gggtatcggc ggaattggtc
     8281 atcagcttca accgttcctg cggcggcagc agcaactgat acgccgccac gcccaaagcc
     8341 gccgccattt tttcgatatt cgacagggcg atgttccagc gtttgcgctc gactgccgac
     8401 acataagtcc tgtccaaacc gcattgccgc gccaattctt cttgcgacca acccttgttc
     8461 acgcggaaaa gccgcatatt gtatgccaat accgcccgca aatcctgttc gtcaggcagt
     8521 ttggcaggcg gggtcaattt gttgcccatc atttgcttcc agataaatgg ttaaagttaa
     8581 gcatttgctg ttacggattt tacttcacat aaaagccaaa aaaaaggggt ggcggcagcc
     8641 acacccaaac acacacacat caagaggaaa gaggtaaaat caagacagat tgggaaacag
     8701 caaacaagca atctagattt catatcggta ttatcaaaca aaaaccgtta tcgagtattg
     8761 attcaaatca atttagattt gattcgcaaa ccggcaaaga aaaacggcat acccgtttat
     8821 acggatatgc cgtctgatga tgcaggggac tgctatattt ggcagtggtt tgtgttttag
     8881 tcctcttgcg ccgctgtttg caggtagttc ggcaaaccaa ttttgccgat cagttcctgc
     8941 tgggtttcaa gccagtcgat gtgttcttcg ttggtgtctt tttgtttttc caacaaatca
     9001 cggctgacgt aatcctgttg cgcttctgct gtggcgatgg cggcaagcag ggcttcgtgt
     9061 ttttcctgtt ctttggtcaa atcgcaggcg atgatttctt cggtggactc gccaatcaga
     9121 agcttgccca gttcttgcag gttcggcaga ccttcgagga acagaatgcg ctcgatcaaa
     9181 tcgtcggcag ctttcatttc aacgatggac tgtttgaaga aatgttcgcc cagttcttca
     9241 aagccccagt ttttcaaaat acgggcgtga aggaaatatt ggttgatggt taccagcagc
     9301 aagcctaagt ttttgtttag ctcgcggata accagacggt cgcctttcat acggactcct
     9361 tttattccga atacggatta cagttggctt tggtagtagt tgccttcgcc aattaactcg
     9421 atcaggcgaa gctgctgttc cagccagtgg gcgtggtctt cttcggtatc tttcagttgg
     9481 gcaaccatca ggtcgcgcgt aacatagtct tgagcctctt cgcacagttt gatgcctttt
     9541 ttcaacgcat cacgtacttc atattcggtt tgcaggtcgg ctttgaggca ggaaaccacg
     9601 tccgtgccga tattcagttc ggcgcgtgcc attttcggcg tgccgcccag catcaggatg
     9661 cggcggatga agtcttcggc gtgtgtggtt tcttcttcca tctcgtggtt gagacgttca
     9721 aaaagtttgg tgtagcccca ttcggagtag aggcgggagt ggataaagta ttggtcgcgt
     9781 gccgccagct cgccagacag caattcgttc atgtaatcta aaactgcttg attgccttgc
     9841 ataatatttc tcttttctta atttgggttt cgtgtagtta aacagtggat gtagcactgt
     9901 tcggaatgcg tgcattgtat gcaaaagctg tcggcatgac aaattttcta tttaaaatac
     9961 aaacaattat caaaagaaat agggcgtatt ccccgatgcc ttccaaatca gtatgctgtt
    10021 ttttatcggt tttttgtgct gttaaaacta ataatccata ttatccataa agaacattta
    10081 cacttgttaa ccatagctaa aagcaaatac ggtacggttt tttatcaaaa tttataatga
    10141 atcgttctca ttaaccgaca gattcttttg agattatcgg attttgggaa taaattatgc
    10201 cgtctgaggg gctttcagac ggcatagaca attaaagctg attaaacaac gcggcagaca
    10261 gcagcgtagg aaattatttt ttacttcgga acggtatttt gggtttaatg atgcccgcaa
    10321 ccgccgtggc tttcgcgtaa ggcttcggta gcctgttcgt cggcgtggta gctggaacgt
    10381 accatcgcgc cgatggcggc attgctgaag cccagttcgt atgcttcttt ttcaaagatt
    10441 ttgaactgct cgggcgtaac gtagcgcagg acgggcaggt gtccgtctga aggctggagg
    10501 tactgtccga tggtaatcat ttcgatattg tgcgcccgca tatcgcgcat aatttcgcgc
    10561 acgtcttcgt ctgtttcgcc caagccgacc atgatgccgg atttggtcgg gatgtgcggc
    10621 atcatttctt tataacgttt taataagtct aaagaatgtt gataattggc accgggacgg
    10681 gcttttttgt acaggctggg atgggtttcc aagttgtggt tcatcacgtc gggcggagtt
    10741 tcggcaagga ttttcagtgc gatgtccaag cggcctcgga agtcggggac gaggatttcg
    10801 attttggtgt tcggactagt ctcgcggatg gctttgatgc agtcggcgaa atgctgtgcg
    10861 ccgccgtcgc gcaggtcgtc gcggtcgacg gaggtaatga cgacataacg caggttcatg
    10921 gctttgacgg attcggcgag gtttttcggt tcgtcggggt cgagcatatt cgggcggccg
    10981 tgtcccacgt cgcagaacgg gcagcggcgg gtgcagatgt cgcccataat catgaaggtc
    11041 gccgtgcctt tgctgaagca ttcgccgatg ttggggcagg aagcttcctc gcaaacggtg
    11101 tgcatttttt gttcgcgcaa aatgtctttg atttcaaaga atttgcgcga tggaagtttg
    11161 gcgcgtatcc attcgggctt ttttaatttt tcctgaaggg ggacgacttt gatggggatg
    11221 cgggcggttt tgtccgcgcc tctgagtttg atgccgcgtt tggggtcgtc ggttttgatt
    11281 tcactcattg ttgtctgctt tcggtgtgaa ttgtgtttca aggtgtgcgg tgagtttggc
    11341 ggcgacttcg tccggcgtgg ggcagggttg gacaaaatcc gcgatttgcg tcatttccat
    11401 accggcgtag ccgcaggggt tgatgtgggt aaacgggctt aaatccatat tgacgttgag
    11461 cgcaagcccg tgatagacgg agccgttttt gatacgcagc cccagtgagg cgattttgcg
    11521 ttctccgaca taaacgccgg ggcgtttggg gtctgccgcc gcttcgatgc cgtattctgc
    11581 caatgtggcg atgatgctgt tttcaagcgc ggaaacgatg tttctaacac tggttttgcg
    11641 ccgtttgaaa tcaatcatcg tataaacgac caattgcccg ggcccgtgat aggtaatctg
    11701 cccgccccgg tcgatttgga cgacgggaat gtcgtcgcga atcagcaggt gttcgggttt
    11761 tcccgccagt ccttgtgtga acacgggcgg gtgttcgacg acccacagtt cgtcttcggt
    11821 gtcggcattc cgtccggcat taaaggtttt catcgcttca aaagtcggca gatattcgac
    11881 caaacctttg tgtatgattt tcatctcaaa gtaccacttt gaccagttcg tgcgaagtca
    11941 gcgcacggta gatgttgtcc aattgttctt ggttttcaac ctttacctgt acggtggcgc
    12001 cagtatagtt gcctttgctg ctcggacgcg tggtgatgtg gtgcgcctgc gtgtcggggg
    12061 cgtgaaggcg gacggtgtcc aataccgcct gctcgaactc gggatgcacc gcgcccatta
    12121 ctttcaatgg gaaggtgcag ggaaattcga tgagggatgt tttgttttct tgttcggtca
    12181 tgatgtgctg ccttgtcgtg tacggtatgc cgtctgaagg cgggtttgcc tttcagacgg
    12241 catcggatgt gcgttatttt agcctaaacc gcgataacag gctatcggga aggcggaggc
    12301 ttttttgacg gcgcggcggt tctgctatac tggcgcacaa tattattttt agaagggtgg
    12361 tttttatgta tcggaggaaa gggcggggca tcaagccgtg gatgggtgcc ggtgcggcgt
    12421 ttgccgcctt ggtctggctg gttttcgcgc tcggcgatac tttgactccg tttgcggttg
    12481 cggcggtgct ggcgtatgta ttggaccctt tggtcgaatg gttgcagaaa aagggtttga
    12541 accgtgcatc cgcttcgatg tctgtgatgg tgttttcctt gattttgttg tttgcattat
    12601 tgttgattat tgtccctatg ctggtcgggc agttcaacaa tttggcatcg cgcctgcccc
    12661 aattaatcgg ttttatgcag aacacgctgc tgccgtggtt gaaaaataca atcggcggat
    12721 atgtggaaat cgatcaggca tctattattg cgtggcttca ggcgcatacg ggcgagttga
    12781 gcaacgcgct taaggcgtgg tttcccgttt tgatgaggca gggcggcaat attgtcagca
    12841 gtatcggcaa cctactgctg ctgcccttgc tgctttatta tttcctgctg gattggcagc
    12901 ggtggtcgtg cggcatcgcc aaactggttc cgaggcgttt tgccggtgct tatacgcgca
    12961 ttacgggtaa tttgaacgag gtattgggcg aatttttgcg cgggcagctt ctggtgatgc
    13021 tgattatggg tttggtttac ggcttggggt tggtgctggt cgggctggat tcggggtttg
    13081 caatcggtat ggttgccggt attttggttt ttgttcccta tttgggcgcg tttacaggac
    13141 tgctgctggc aaccgtcgcc gccttgctcc agttcggttc gtggaacggc atcctatcgg
    13201 tttgggcggt ttttgccgta ggacagtttc tcgaaagttt tttcattacg ccgaaaatcg
    13261 tgggagaccg tatcgggctg tcgccgtttt gggttatctt ttccctgatg gcgttcggac
    13321 agctgatggg tttcgtcgga atgttggcag gattgccttt ggccgccgta accttggtct
    13381 tgcttcgcga gggcgtgcag aaatattttg ccggcagttt ttaccggggc aggtaggcgg
    13441 ttccgaaaca tatttgaagc gggatacaac cctgcccggg t
//
LOCUS       DAXHPG010000038        13447 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXHPG010000038 DAXHPG010000000
VERSION     DAXHPG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13447
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.038"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(125..217)
                     /locus_tag="ACFM6F_000833"
     tRNA            complement(125..217)
                     /locus_tag="ACFM6F_000833"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(181..183),aa:Ser,seq:gct)
     gene            complement(374..574)
                     /locus_tag="ACFM6F_000834"
                     /pseudo
     CDS             complement(374..574)
                     /locus_tag="ACFM6F_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            complement(676..993)
                     /locus_tag="ACFM6F_000835"
                     /pseudo
     CDS             complement(676..993)
                     /locus_tag="ACFM6F_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            complement(1016..1777)
                     /gene="kdsB"
                     /locus_tag="ACFM6F_000836"
     CDS             complement(1016..1777)
                     /gene="kdsB"
                     /locus_tag="ACFM6F_000836"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC6164287.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTVCQAHGVEVVMTSNRHESGTTRLAEAAAALKLPQHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYLRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRIRAVFQTI"
     gene            complement(1774..1956)
                     /locus_tag="ACFM6F_000837"
     CDS             complement(1774..1956)
                     /locus_tag="ACFM6F_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC6164288.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARTLSEEELKA"
     gene            complement(2025..2606)
                     /locus_tag="ACFM6F_000838"
     CDS             complement(2025..2606)
                     /locus_tag="ACFM6F_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC6164289.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            complement(2812..3846)
                     /gene="lpxK"
                     /locus_tag="ACFM6F_000839"
     CDS             complement(2812..3846)
                     /gene="lpxK"
                     /locus_tag="ACFM6F_000839"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC6164290.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAASRAEDAGDEPLLLFSKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLDSVDTVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            complement(3977..4156)
                     /locus_tag="ACFM6F_000840"
     CDS             complement(3977..4156)
                     /locus_tag="ACFM6F_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164291.1"
                     /translation="MKEMETVRYHIGDMPETSKQTAPVMTTGQWVLTMIVFMIPLVNF
                     CLGVRQRQPEPRQFL"
     gene            complement(4239..5519)
                     /locus_tag="ACFM6F_000841"
     CDS             complement(4239..5519)
                     /locus_tag="ACFM6F_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC6164292.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATSKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLVDAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRSCVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADFEAYAAKLGEWKL"
     gene            complement(5741..6361)
                     /gene="tmk"
                     /locus_tag="ACFM6F_000842"
     CDS             complement(5741..6361)
                     /gene="tmk"
                     /locus_tag="ACFM6F_000842"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC6164293.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            complement(6421..7416)
                     /gene="mltG"
                     /locus_tag="ACFM6F_000843"
     CDS             complement(6421..7416)
                     /gene="mltG"
                     /locus_tag="ACFM6F_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC6164294.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLYTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            7500..8072
                     /gene="ampD"
                     /locus_tag="ACFM6F_000844"
     CDS             7500..8072
                     /gene="ampD"
                     /locus_tag="ACFM6F_000844"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC6164295.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            8266..9552
                     /locus_tag="ACFM6F_000845"
     CDS             8266..9552
                     /locus_tag="ACFM6F_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC6164296.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVCDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            9700..12225
                     /gene="ligA"
                     /locus_tag="ACFM6F_000846"
     CDS             9700..12225
                     /gene="ligA"
                     /locus_tag="ACFM6F_000846"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC6164297.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVANIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETTDKTVAESDQTPSEG
                     SSVGASGKHKKQPVKWAENILGGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            12282..>13447
                     /gene="hemW"
                     /locus_tag="ACFM6F_000847"
     CDS             12282..>13447
                     /gene="hemW"
                     /locus_tag="ACFM6F_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC6164298.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALESAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQNRPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQ"
BASE COUNT         3177 a         3921 c         3346 g         3003 t
ORIGIN      
        1 tgttaatcca ctataacaaa attcagaatc aagacagggc aataccatac tggttttgca
       61 ccaacccgct ataaaattgc ataaaaaaaa tagacgctgt aaaacacagc gtctattttt
      121 tatctggcgg agtaagaggg attcgaaccc tcgatgcagg ttgaccccac atgcttcctt
      181 agcagggaag tgccttcagc ctctcagcca ttactcctaa ggaagctgta actataatca
      241 acttttccga taccgtcaag gaaaaatgga aagcaaatat taatcttttg aaataaaata
      301 agatttaccc tgattatacc caatcaggca cttcttttct tcctgaatgg aaatattcct
      361 gactgccaca tcctcaagca aaatgctttt gtgcatcctg tttgatttcc tgaaaccacg
      421 cgtcttcagg aaaacgtgca agcgcggcat ccaatgttga cagtaggttt tgcgacaggg
      481 ctttggtcag gtattcctcc tcgttggctt ctgccgcttt gtcgggctgt tgtgccaaac
      541 cgtcggcacg ggctttgtgt ccgtgtgcca aatatagtgg attaacaaaa atcaggacaa
      601 ggcgacgaag ccgcagacag tacagatagt acgacaaggc gaggcaacgc cgtactggtt
      661 taaatttaat ccactataat tgagcgcggt aattttttgc gcccaaagct gcggtatgtc
      721 gggattgtct tcaatcatga cggaaaggat ggatacggcg gtcgccagcc tgccgccgtt
      781 cattctaagg tcgagggaac ggttgggcgg cagggtgctg agtgtttctg ccattctgaa
      841 ccaaaattcg ccactggctt cgtcgggtgc gttcaatttg agcatttcat agtgaacctc
      901 ctgatcatcg ttttgcacga gaccccatac ttcagcaagg attttctgtt cttcccggct
      961 caagtgttgc catttttcaa tatgttgctg catatctttt ccctttgaat ctattttata
     1021 tagtctgaaa tacagcgcga atcctgtcca aatcctcctg cgtatctacg ccggcggcag
     1081 gagcttcttt ggcggtttcg acggcaatcg ggtagccgtg ccacaggacg cgcagttgct
     1141 ccagcgattc aatggtttcc agcggcgaaa cgctcatttc ggtgtagcgt tgcaggaagc
     1201 cgacgcggta ggcgtagatg ccgatatggc gcaggacggc ggtttcaaac ggcatttcgc
     1261 gttttccggc acgcattgca tcgcgcagat agggaatcgg ggcgcggctg aagtagatgg
     1321 cgttgcggtt tttgtcgagg acgactttga cggcattggg attcatcaat tcgtcgaaat
     1381 cgtgcaattc gtgggcggcg gtcgccattt ggacgttgtt ttcgacgagt acttcggcgg
     1441 tgcggtcgat gagttcgggg gcaatcagcg gctcgtcgcc ctgtacgttg acaacgacca
     1501 gatgttgcgg cagcttcagc gcggcggcag cttcagcaag gcgcgtcgtg ccgctttcgt
     1561 gccggtttga agtcatgacg acttcgacgc cgtgcgcctg acagaccgtc tgaatatcgg
     1621 gatggtcggt ggcaacaacg acgcgcgcag ctttactttt tgccgcctgt tcggcaacgc
     1681 gcacgaccat cggtttgccg tggatgtccg ccaaggcttt tccgggcagg cgcgacgaat
     1741 ccagccgcgc cggaatcaat acgacgaatt cggtcatgct ttgagttcct cttcgctcaa
     1801 cgttcgcgct tcgttttcca gcatataggg aatgccgtct ttaatcggat aggcaagctt
     1861 cgcctgacgg ctccacaatt cctgtttgtc ctgatgatat tccagcctgc ctttggtaac
     1921 ggggcagacg aggatgtcta agaatttttt ttccataggg ttgctttcgg gtttgatggt
     1981 tcaatcaatc ggatgccctc ttcaatattt cagacggcta tcatttattt ctgatgcctt
     2041 ttcccaacct gtccggcttg tttacagccc gcatcgggat ttttaccgcc gcagatgatg
     2101 cgccgcaact cttccgtaaa cgcgggcaga tgatgtcgcg cgattttccc tgacaatgca
     2161 gtccaagata ctgctatttc actcatcgat ttcttgatta agcgcggatt tttggcaacg
     2221 acggactgac cgacaggcga accgtaaaag gcaatcatgc cgtcaatttc ttcttgcgtg
     2281 tatatctcac gggcattctt caataaagta ttgcggacag cctgtttcac ttcgggcgta
     2341 atcaaatctt tcaaaacatt ctcacgatag cggttaaagg cttttgccgc ctgatctttt
     2401 ttcgcttccg gcatttcggc aagggctttg tccgcatacg gtttaaatcc ggcattaaag
     2461 ccctcaatca tatttttttc tatatcccgg tcaaaattct gcgtatccag ccaacgcgcc
     2521 aacgacgcgt cgctgggcgg ggcggcaaaa gcgttggtgc acaatgccag cgtggcgaag
     2581 ggcaataaca aggttttcag tttaatattc aatcctttaa cgaatgatta atgcagataa
     2641 aaaatacgac cgattttctc attcctgtcc aagtggaaat ccggagattt gggatttctg
     2701 aagcctccta ggcattcctg aaaattcgag acttagattc ccgcctgcgc gggaatgacg
     2761 gcgcgaggcg ttcttattca aatccgtaat cactccgccc aaaccgtgct ttcagacggc
     2821 cttcggtaca tcttccaacc gctccaacac aaacgccgcc aaatcaggtt cgattatcgc
     2881 acaaacgggc aacacccaaa cattatcggt gcaaatgccg tctgaaaatt tgaccgcatc
     2941 tttctccgta ataatgaccg cgtccgcatc gggcaaatct gccgccgaaa tgtcggcgtg
     3001 gtcgggcagc gcgacggttc gcttcacggt aatgcccata ttccgcaacg aatcgaaaaa
     3061 acgcgccggc ttggcaatac ctgccacggc gacgacggtt tgatttttca gacggcctgt
     3121 gtccagtatt tcggacggat tgttcaaacg gtaaattcgt cccgcttcga tacggctgtg
     3181 aaacatattt tcagacggcc taaacagcgc atccgccttg ccgccgctga cgacgaccgt
     3241 atccaccgaa tccagccgcg acaaaggttc gcgcaaattg ccgttgggca gcaaatccaa
     3301 atccgtgcgc cccgtatccg ccgccggaaa caccgcgatt tccacatctc gccgcagggc
     3361 gtaatgctgc aaaccgtcgt ccgccacaat cagtccgatg tcgggatgcg ccgccagcaa
     3421 cgccctgcct gcctctgcac ggctgctgcc caccgccgtc ggcgcaccgg ttttgctgaa
     3481 cagcagcaaa ggctcgtcgc ccgcatcttc cgcacggctc gcggcattca atacatgaac
     3541 cgccttgctc ttgcgcccgt agcctcggct gatgatgccg accttgacac ctttttcctg
     3601 caaacccgac accagcgcgg cggcaatcgg cgttttcccc gtcccgcccg cgtggatgtt
     3661 gccgaccacg accacaggca cgggcagctt ttcgctttgc cgttttcccg ataaaaaatc
     3721 cgtccgccgt tttgccgcaa ttttggcaaa cagcccggag agcggcttaa gcagaaaaga
     3781 caaaaccgga taaggttttt gccaatgccg ttcgataacg gtatgaaatt tgaaacaatt
     3841 cggcatattc gatacaaatc ctcgaagcaa aatcagcccg gggcattaat catgcgtacc
     3901 cgatacagac ccacctataa acaacgcgaa gaccaaaccg ataagcgaac caatcaggta
     3961 aataagcaac tgcgctttac agaaattggc gcggttcggg ttgcctctgc cgaacaccca
     4021 aacaaaaatt gaccaaagga atcatgaaaa caatcatcgt caacacccac tgccctgtcg
     4081 tcatgacagg ggcggtttgt tttgaagtct cgggcatatc gccgatatga taacggacag
     4141 tctccatctc tttcattttc cagtatcttt catttatcaa tctgacagca aaatgccgcc
     4201 tgaaaacagt tcagacggca ttttaaaccg caaacggctt acagcttcca ttcgcccagc
     4261 ttggcggcgt aggcttcaaa gtcggcaatc ggacgggttg ccacgccgct ttccatcgct
     4321 gctttggcgg cagccgtagc aacgcgcggc agcaggcggg aatcgaacgg agtcggaatc
     4381 aggtattccg cgccgaattc aaatttctta ccgtaagcgg caaccacttc ttcggttact
     4441 tcttccatcg ccaaatccgc caaagcatac acgcaggagc gtttcatttc ttcgttgatg
     4501 gtcgtcgcgc cgacatccaa cgcgccacgg aagatgaatg ggaagcacaa tacgttgttc
     4561 acttggttcg ggaagtcgga acggccggta ccgataacca cgtccggacg ggtttctttt
     4621 gccagcggcg gcaggatttc cggattcggg ttggccatgg cgaacacgat gggtttttcg
     4681 ttcatcgtgt tcaacatttc gggggtcagc aggttcgcgc cggagaggcc caagaagatg
     4741 tctttacctt tgacggcatc gacaagtacg cgctggccat tgtcttcaac ggcgtagaat
     4801 tttttggatt cgtccatgcg atctttgtct tcgcgggttt ggtaaatcac gcctttggag
     4861 tcgcaaacgg tcacgttttc gcgtttcaag cccaaatcca gcaattggtt caagcaggca
     4921 atcgcggccg cacctgcgcc ggaacacacc aaagtcgctt cttcgatttt acggccggta
     4981 aaacgcaggg cgttcaatac ggcagcggcg gtaatgatgg ccgtgccgtg ctggtcgtcg
     5041 tggaatacgg ggattttgca gcgtttgcgt aattcgcgtt cgatgtagaa acactcgggt
     5101 gctttgatgt cttcgaggtt gatgccgccg aaggtcggct ctaaagcggc gatgatgtcc
     5161 acgagttttt gcgggtcttt ttcatcgatt tcgatgtcga acacgtccac accggcgaat
     5221 tttttgaaca atacgccttt accttccatc acgggtttgc ccgccagcgc gccgatgtcg
     5281 cccaagccca aaacggccgt accgttggaa atgacggcga ccaagttgcc tttggcggtg
     5341 tatttgtagg cattttgcgg atcggcatgg atttccatac aaggagcggc tacgcccgga
     5401 gagtacgcca acgccaggtc tttagacgtg gccagagatt tggtcggggt aacggagatt
     5461 ttgcccggaa cgggtaattc gtggaacttg agggcggctt ctttcaatga gttttccatg
     5521 tgtcaatcct gttgcgagaa aaagatttga atcggactgt ccgaaacgct ttcgggtcat
     5581 catgttacgg gcggttcgtt aatattgccg acgcatcctg ccttgtaacc gagcgtaatt
     5641 ctatcatgcc gaaagtttgg cgcataccgc cattttcacc tgcggcactg ccgtttgatt
     5701 ttcggcgcgg gtggatgcgc ttgtttcaat atttgccgca ttaacagccg aaatgtccgt
     5761 ccaacacttt ttctatgttg tttctgactt catccaagct gcggttactg tcgataatgg
     5821 cgtaccgttc gggacaggcg gcggcacggt tcagataaac gctgcgcaca cgcatgaaga
     5881 aatctgcctg ttcctgctcg aaccggtctt tctcgcgcga ctgtccgata cgcgccatcg
     5941 acacttccag cggcacatcc aacagcaggg tcaaatcggg gcgcaaaccg ccctgcaccc
     6001 aatgttccaa aatttcaatg tcttcagacg gcatcccccg cccgccgccc tgataggcga
     6061 aggttgcgtc ggtaaaacgg tcggacacga catggatgcc gtctgaaagt gcaggcaata
     6121 tcacgtcttc aatatgctgc attctggcgg cgaacatcat cagtgtttcc gcacgcaaac
     6181 cggctttggt ttcagggttg agcaggattt cgcgcaaggc ctcaccgacc ggcgttccgc
     6241 ccggttcgcg cgtgaacagc acgggcagcc ccctccgttc aaaccatgcc ttgatgacgg
     6301 caaggttggt ggatttgccg gcaccgtcta tgccgtccaa agtgatgaat tgcggtttca
     6361 tgcttgcagt tccggtaagg gtatgccgtc cgaaaacaca aacttttcag acggcatggt
     6421 ttattttttc aaaatatatt tgcgaacggc ggcgttgtgt tcggtcaaat catggctgaa
     6481 ctggctcaag cccgtaccgt ccattttgga cacgaaatac aggtattttt caccggacgg
     6541 atgggcggcg gcatcgagtg ccgccttgcc gggcagcgcg atcggggttg gcggcagacc
     6601 gccgcgcgtg taggtgttgt acggcgtgtc gcggcgcagg tcggctttac ggattttgcc
     6661 cttgtatgcc gcacccatgc cgtaaatcac ggacgggtcg gtttgcaggc gcataccgat
     6721 tttcaggcgg ttgacgaaga cggaagcgac atggtcgcgg tcggcttcat gccctgtttc
     6781 cttttcgatc aggctcgcca taatcagcat ttcataaggg tttttataag gcagcccgtc
     6841 ctgcctgctt tcccatgcct cattcagtcg gcgttgcatc gccttgtagg cgatttggta
     6901 aatccgtaaa tcgctgccgc ccgcatcgat ttcgtagctg tcggggaaaa actgcccttc
     6961 aggattgccg ctgaaggcat caggggcaac ttccgccatc agtttttcat tgctccagcc
     7021 tttggtgtcg tgttcgatgt cgggcgttgc gtcgatgact ttcctcatat gcgaaaaacg
     7081 cgaaccttcg ataatctgca cggtaacgga atccggcctg ccgccgcgca ttttctgcaa
     7141 gatatcccaa gcagacactt ccgaaggcag tctgtacgtc cccgtataca gcctgttgtg
     7201 cacacccaaa acgtaggccg ccgccgtcaa aacatgcctg ctgaacacga tgcggtcttc
     7261 ggcaagtttc ctgccgaccg acgaaatacc ctggtttttg gcaattttaa tcctgtatgc
     7321 cctgccgttg tctttaggga cgaaaagcag cgcggcgaaa acggctgccg atacggtcaa
     7381 aaaaacggca gaccatttca acaattttct caacatggta ggattcccag cattcggaat
     7441 acgtcttaaa attcgggcga cagtataacc caaattgccc gccggacagg aagaacatca
     7501 tggacaatca cgccgaagca cactggcaaa acggctggct tcaaagcata cgccataccc
     7561 cgtcgcccaa tttcagcccg agggaaacgg gggaaacggt ttccctgatc gtgttgcaca
     7621 acatttcgct gccgccgttc gaatatggta cggatgcggt ggaaaagctg tttgccaacc
     7681 ggctcgaccc cgacggacat ccgttcttca gcctgataca cactttgcgc gtatccagcc
     7741 attttttaat caaacgcgac ggcgaaacgg tacagttcgt ttcctgcgac aatatggcat
     7801 accatgccgg cgtatcctcg tttcgcggac gggaaaaatg caacgcattt tccatcggca
     7861 tcgaactgga aggctgcgat ttcgaaccct ttaccgaagc gcaataccgt tcgctcgaag
     7921 ccttgttgga cgcaatctgc cgccgctacc ccatcacggc ggtaacgggg caccaagaca
     7981 tcgcgcccgg ccgcaaaacc gaccccggtc attttttcga ctggcggcgg atacggggga
     8041 aagggtttcc cgtggacaga aatgccgtct gaaaaccaca gattttagat ggcatttcgg
     8101 aaaatttccg cttatttcga cacactgctg tcagaccctg caattttcgt tataattcaa
     8161 acgttataac cggccgttcc gtcagactgc cggaaacgca agcccgattt aaccgcagta
     8221 cccgcacttt gcgcatcctg catcccgaaa tttaaagtga ttgccatgat ttacatcgta
     8281 ctgttcctcg ccgccgtcct cgccgttgtc gcctacaata tgtatcagga aaaccaatac
     8341 cgcaaaaaag tgtgcgacca gttcggacac tccgacaaag atgccctgct caacagcaaa
     8401 accagccatg tccgcgacgg caaaccgtcc ggcgggtcag tcatgatgcc gaaaccccaa
     8461 ccggcggtca aaaaaacggc aaaaccccaa gaccccgcca tgcgcaacct gcaagaacag
     8521 gatgccgtct acatcgccaa gcagaaacag gcaaaagcct ccccgttcaa aaccgaaatc
     8581 gaaaccgcct tggaagaaag cggcattatc ggcaactccg cccacaccgt ttccgaaccc
     8641 caaaccggac attccgcacc gaaacctgcc gacgcgccgg caaaacctgc acccgttccg
     8701 caaacacctg caaaaccgct gattacgctc aaagaactgt caaaagtcga attaccctgg
     8761 tttgacgtgc gcttcgactt catctcctat atcgcgctga ccgaagccaa agaactgcac
     8821 gcactgccgc gcctttccaa ccgctgccgc taccagattg tcggctgcac catggacgac
     8881 catttccaga ttgccgaacc catcccgggc atccgctatc aggcatttat cgtgggtatt
     8941 caggcagtca gccgcaacgg acttgcctcg caggaagaac tctccgcatt caaccgccag
     9001 gtggacgcat tcgcacaaag catgggcggt cagacgctgc acaccgacct tgccgccttt
     9061 atcgaagtgg cttccgcact ggacgcattc tgcgcgcgcg tcgaccagac catcgccatc
     9121 catttggttt ccccgaccag catcagcggc gtagaactgc gttccgccgt aacgggcgtg
     9181 ggtttcgtcc tggaagacga cggcgcgttc cactataccg acacgtcggg ctcgaccatg
     9241 ttctccatct gctcgctcaa caacgagccg tttaccaatg cccttttgga caaccaatcc
     9301 tataaaggct tcagtatgct gctcgatatt ccgcactctc cggcaggcga aaaaaccttc
     9361 gacgatttgt ttatggattt ggcggtacgc ctgtccggcc agttgaacct gaatctggtc
     9421 aacgacaaaa tggaagaagt ttcaacccaa tggctcaaag acgtacgcac ttatgtattg
     9481 gctcgtcagt ccgagatgct caaagtcggt atcgaaccgg gcggcaaaac cgcattgcgc
     9541 ctgttctcct aaaatcgaat gcaatcaaag cggatatgtc gttacggcat atccgctttt
     9601 tttcgggtaa aattcagctg ttttcaacct atcatttttc agacgacctt tgcccgtttc
     9661 atcccgtatc agacaaaggt cgtctgaaaa ccttctctca tgaatccaaa ctcaaaacat
     9721 aacactaact ttaccaatct tcttaaaccg tctgatagtg atataaaaca atttgccgca
     9781 caacacatcc gccacctcac cgacctgctc aaccgctatg cctacgaata ctacaccctc
     9841 gacgcgccca gcgtacccga tgccgaatac gacaaattat tccgcgagct cgaagcgttg
     9901 gaattaaacc atcccgagct caaactgccc gacagtccga cccagcgcgt cggcggcgag
     9961 cctttagcgg gatttgccga agtgcgccac gaagtgccga tgctgtcgct gaccaacgct
    10021 ttttccccgc aagatgaaaa cggcgtgttc gaccatgccg aaatgtacgc tttcgaccaa
    10081 cgcgtgcgcg acggtttgga cggcggcaaa cccgaatacg ttatcgaacc caaattcgac
    10141 ggcctcgcca tcagcctgct ctaccgcgac ggcgtattgg tacaggcggc aacgcgcggc
    10201 gacggcacaa cgggcgaaga cgttacccaa aacatcaaaa ccgtcgccaa catccccctg
    10261 cggctgcacg gcgaaaatac gcccgaactc atcgaagtac gcggcgaagt actgatgcaa
    10321 aaagccgatt ttgccgccct caataaaaga caagccgaaa acgggcaaaa accctttgcc
    10381 aatccgcgca acgccgctgc cggcagcctg cgccaactcg attcgcgcat cactgcgcaa
    10441 cgcaaactgc actttttccc ctactccgtc gcccgccagc aaggcggttt cgtcgcggaa
    10501 gaacacatcc aagaactcgc ctatttccaa gcactcggct tcagtctgcc aaatggcaat
    10561 ttcggctgtt tcaaaaatat cgacgaagta ttggcgtttt acgaacaaat gcagcaaaaa
    10621 cgccccgaac tgccctacga aatcgacggt atggtggtca aagtcaacag cttggcgcaa
    10681 cagcgcgaac tcggcttcat ctcacgcgcg ccacgctggg cgattgccca caaattccct
    10741 gccgaagaag ccctgaccat tgtcgaagcg attgacgtgc aaatcggcag gacgggcgcg
    10801 gtaacgccgg ttgcccgatt acaacccgta ttcgtcggcg gcgtaaccgt taccaacgcc
    10861 accctgcaca atcaggacga agtttcccga aaagacgtgc gcgtcggcga taccgtcgtc
    10921 gtgcgccgcg ctggagacgt gattcccgaa gtcgtgcgtg tgatttttga acgccgcccg
    10981 atgcaggaaa ccgccgttgc tgtttcagac ggcatcgggc atcggcagga cgatttgttt
    11041 gccgaaacac cgtccgctaa tcaaacccaa tccgttccgc tccacaagcc ctaccgcctg
    11101 ccgacccatt gccccatctg ccgcagcgaa atcgaacgcg aagaaggcga agccgtcgcc
    11161 cgatgcagcg gcggtatgct ttgtcaggca caacgcgcgc aaggcttaat ccacttcgcc
    11221 tcgcgcaaag ccatggacat cgacggcttg ggtgaaaaac aaatcgaaca gcttgtcgcc
    11281 caagacctcg tccgccactt cgccgacctc taccgcctcg atattccgac cctgcaaaaa
    11341 atgaaagaaa cgacggacaa aaccgttgcc gaatcggatc aaacgccgtc tgaaggcagt
    11401 tcggtcggcg cgtcgggcaa acataaaaaa caaccggtca agtgggcgga aaacatcctc
    11461 gggggcatag aatccggcaa aacgcccgaa ctcgcgcgct tcctgttcgc gctcggcatc
    11521 cgccacgtcg gcgaacgcac cgccaaaacg ctggcgcagg cattcggcac attggaacgc
    11581 gtccgccgcg cccccgaacc cgtcctcgcc tgcctgcccg acatcggtac ggtagtggcg
    11641 cattccattg cccacttctt cgcccaagcc gaacagcagg cgatgataga cgaactgctc
    11701 gccgcaggcg ttgccccgca aacccaagcc gttaccatcc cgcccgcccg ccacgccgaa
    11761 ccgcaacgct ggatcgcccg cctgcccgac ttcaaaatta gcgaaaacaa agcccaagcc
    11821 ttatgggaac tcgctggtaa aaacatagaa ggtctacaaa acgacaaagc cctccccgcc
    11881 gactggcaag catggcgcag cgaaccacaa aacgccgccc tactcgaaaa cctgaaaacc
    11941 ttcttcgcgc aaatgccgtc tgaagacgag gcggcgcaag gttcagacgg catcaataaa
    12001 gccgtagcag gcaaaacctt tgtattaacc ggcaccctgc ccacgctcaa acgcgaccaa
    12061 gcccaatccc taatcgaagc cgcaggcggc aaagtttccg gcagcgtgtc caaaaaaacc
    12121 gactacgtcg tcgccggaga agccgcgggc agcaagctgg aaaaagccaa tgccttgggc
    12181 gtttccgtcc tcagcgaagc cgaactgctt accctgctcg gctgaaccta tgccgtctga
    12241 accgccttca gacggcattg tccacttttc cgaaccacac catgcacacc atttcctttc
    12301 caaaccgcac ccgactcacc gccctgccgc ccctgtcgct ttacatccac atcccgtggt
    12361 gcatcaaaaa atgcccgtat tgcgacttta attcccacag cctgaaaaac ggattgcccg
    12421 aagccgccta tatcgacgcg ctgctgaccg acttgcagct cgaattgccc aatatttggg
    12481 gcaggccggt ggaaacgatc tttttcggcg gcggtacgcc cagcctgttt caggcggaat
    12541 cgattgaccg tttattgagc ggcgtgcgtt cgctgttgcg cttgcagccg gaagcggaaa
    12601 ttaccttgga agccaatccg gggacatttg aaatcgagaa gtttcaagga tttaaagacg
    12661 caggcatcac gcggctttct attggtgtac agagtttcaa cgacgatatg ctctcaaggc
    12721 tggggcgcgt ccacaacggc aaggaagcct taaccgctat cgataccgcc ttgaaattat
    12781 ttgataaagt caatatcgac ttgatgtatg ccctgccgaa ccaaaccgtt cagacggcat
    12841 tggacgacgt acaaaccgct atcgctacgg gcgcgaccca catcagcgcg tatcatctga
    12901 cgatggaacc gaacacgccg ttcggtcata cgccgccgaa aggtttgccg caagacgaag
    12961 ccgccctcga catcgaagac gcggtacacg gcgcgctgga aagcgcgggt tttgtccact
    13021 acgaaacatc ggcttttgcg aaaccagcca tgcagtgccg ccacaatttg aactactggc
    13081 agttcggcga ttatttaggc ataggcgcgg gcgcgcacgg caaaatttcc tatcccgacc
    13141 gcatcgagcg caccgtccgc cgccgccacc ccaacgacta cctcgcctta atgcaaaacc
    13201 gaccgagcga agccgtcgaa cgcaaaaccg tcgccgccga agatttgccg tttgagttca
    13261 tgatgaacgc cctgcgcctg accgacggcg tacccgccgc gatgttgcag gaacgcacgg
    13321 gcgtaccgag tgccaaaatc atggcgcaaa tcgaaacagc aaggcaaaaa ggcctgctgg
    13381 aaaccgaccc cgccgtattc cgcccgaccg aaaaaggacg cttgttttta aacgatttgc
    13441 tgcaatg
//
LOCUS       DAXHPG010000039        13099 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXHPG010000039 DAXHPG010000000
VERSION     DAXHPG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.039"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(310..1206)
                     /gene="argB"
                     /locus_tag="ACFM6F_000848"
     CDS             complement(310..1206)
                     /gene="argB"
                     /locus_tag="ACFM6F_000848"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC6164299.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1376..2506
                     /locus_tag="ACFM6F_000849"
     CDS             1376..2506
                     /locus_tag="ACFM6F_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC6164300.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2577..3428
                     /gene="lgt"
                     /locus_tag="ACFM6F_000850"
     CDS             2577..3428
                     /gene="lgt"
                     /locus_tag="ACFM6F_000850"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC6164301.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3511..4173)
                     /locus_tag="ACFM6F_000851"
     CDS             complement(3511..4173)
                     /locus_tag="ACFM6F_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC6164302.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4271..6064)
                     /locus_tag="ACFM6F_000852"
     CDS             complement(4271..6064)
                     /locus_tag="ACFM6F_000852"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC6164303.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKRHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNTESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6303..7412
                     /gene="proB"
                     /locus_tag="ACFM6F_000853"
     CDS             6303..7412
                     /gene="proB"
                     /locus_tag="ACFM6F_000853"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC6164304.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPAKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     NRTDFADKRRYQNAGGALSVLLQRRAIPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDRIEHINHEIIEMAGGAGSSNGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGVAGIEGHFSRMDTVTVYSNATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7425..8687
                     /locus_tag="ACFM6F_000854"
     CDS             7425..8687
                     /locus_tag="ACFM6F_000854"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC6164305.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRLDLEAAAGKIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KAGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIKAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIIRGVGQVRE"
     gene            complement(8759..11791)
                     /locus_tag="ACFM6F_000855"
     CDS             complement(8759..11791)
                     /locus_tag="ACFM6F_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6164306.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPTGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDTEADE
                     GAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETILGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            11956..12636
                     /locus_tag="ACFM6F_000856"
     CDS             11956..12636
                     /locus_tag="ACFM6F_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFC6164307.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            12665..13024
                     /locus_tag="ACFM6F_000857"
     CDS             12665..13024
                     /locus_tag="ACFM6F_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFC6164308.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT         2869 a         3744 c         3379 g         3107 t
ORIGIN      
        1 aatctaaaga aacctttttt cccgataagt ttccgtgccg acaggtctag attcccgcct
       61 gcgcgggaat gacgaaattt caaagttatg gcgttatcgg aaaaacagaa aatcaaagcc
      121 ggagaattta tcccaaacaa ctggatttca aaaaaaccag attcccgcct tcgcgggaat
      181 gacggatttt aggtttctgt tttggtttct gttttcgcgg gaataacaac ttagaaattg
      241 cccgaaaccc caaaaacaga aaccggacaa acgggttttc cgccaaagcc ggcattttcc
      301 gactttgctt caggcatctt ccccaccgcc caaaatcatc gaaccgatac cggcatcggt
      361 aaagatttcc agcaaaagcg cgttgggcaa cctgccgtcg atgatatgcg tggctttcac
      421 accgttgacg gcggcttcga ccgcagaagc gattttcggc agcataccgc catacagcgt
      481 gccgtcggca atcagttcat caatccgttt cggcgtgagt ttggtcagca gattgcccgt
      541 tttgtccatc acaccggcga tattcgtcat catcaagagt ttttcagcgt ttaattcttc
      601 cgccaatttg cctgccacca aatcggcgtt gatgttgaac gcttcgcctt tttcacctac
      661 gccgacgggg gcgacgacgg gaatgcagcc acgttctatc agccctttaa ccaaacctgt
      721 atcaatgctt tccaccgtac cgacctgtcc gatgtccacg ccattctgtt cgggcgtatc
      781 gatcaaaagt ttcttcgcct taatgaaatg atcgtcgcgt ccgctcacgc cgaccgcgtg
      841 tccgccatat gtgttaatca tcgacacgat ttctttattg acatgcccac ccaacaccat
      901 ttcgacaata tccatcgcct ctttgtcggt aacgcgcatt ccttggacaa actccccctt
      961 tttgccgact ttttcaagca tcgcattgat ctgcggcccg ccgccgtgaa cgatgacggg
     1021 atgaatgccg accagcttca gcagtacgac atcgcgggca aacccttctt tcaaggcagg
     1081 ttcggtcatc gcgttgccgc cgtacttgat gacggcgacc gaaccggaaa accggcggat
     1141 gtaaggcagc gcttcggcaa ggatacgcgc tttgtcggcg gcggaaataa tgttttcaga
     1201 ctccataacg gctccctatt gttgacaatt tcggcttatg ttaacgccaa acgccgtctg
     1261 aaacaatctt cagacggcat ccggcagacc ttcgggcagg ttgaagcctt gcgatacggg
     1321 cggtaaaatc acagtttatc cggttcgaga aaccggcgac agcaaggact ttcccatgat
     1381 taaaatcagc acccgcttcg atgccggctc ggtcgtcgtc aaagacctga ccgatccttc
     1441 caacatccgc ctcgccctgc gtcccgacaa cgcctccgat tttgcacaat ggttctactt
     1501 ccggctgcaa ggcgcggcat atcaaaactg catcatgcat tttgaaaacg cggcagaagc
     1561 cgcctacccg aaaggctggg aaggctatca ggcgtgtgcc tcatacgacc gccgcaactg
     1621 gttccgcgta ccgacttcct acgaaaacgg cgtactgacc gtcaatcata cgccgctgtc
     1681 caacagcgtg tattacgcct atttcgaacc ttactccgaa gaacagcatt taaacctcct
     1741 cggcgacgcg caaggcagcg gcctgtgccg catcgacgac ttgggcagca ccgtgcaagg
     1801 gcgcgacatc aatctgctga ccatcggcaa tcaggtcgaa agcgatctga aaatctggat
     1861 taccgcccgc cagcatccgg gcgaaaccat ggcggaatgg tttgtcgagg ggctgctcgg
     1921 caggctgctc gatccgcaag acccgacagc gcgcaccctg ctcgaccgcg ccacgttcta
     1981 tatcgtcccc aatatgaacc ccgacggttc ggcactgggc aaccttcgta ccaacgccgc
     2041 cggtgcgaac ctcaaccgcg aatgggaaaa tccgactttg gaaaaaagcc ccgaagtgtt
     2101 cttcgtgcgc gaaaaaatgc tggaaaccgg cgtggatttg ttcttggaca tccacggcga
     2161 cgaagggctg ccctttatct ttgttgcagg aactgaaggc gtgccaaact acaatccgcg
     2221 catttccgcg ttggaaacac agttcaaaac cgcccttttg aacgccagcc ccgatttcca
     2281 agacgaatac ggttacgaaa aagatgcgcc cggtcaggca aacatgacct tggcaacaaa
     2341 ctgggtcggc aaccgtttca actgccttgc ctatacgttg gaaatgccgt tcaaagacaa
     2401 tgccaacctg cccgacgacg atttcggatg gaacggtcaa cggtcgttgc gcttgggcga
     2461 agccgcgttg tccgccatcc tcaacatcat cggcgatttg cgctgaaacc cttattgcag
     2521 aaatgccgtc tgaagcatca tgcctttcag acggcatttg aaaacgaaag cccgttatga
     2581 ttacccatcc ccaattcgac cccgtcctta tcagtatcgg cccgcttgcc gtccgctggt
     2641 atgccctaag ctacatcctc ggatttattc tttttacctt tctcggcaga aggcgcatcg
     2701 cgcaaggctt gtccgttttt accaaagaat cgctcgacga cttcctgaca tggggcattt
     2761 tgggcgtaat tttgggcggg cgtttgggtt acgtcctgtt ttacaagttt tccgactacc
     2821 tcgcccatcc gcttgatatt ttcaaggtat gggaaggcgg aatgtcgttc cacggcggct
     2881 ttttgggtgt agttattgcc atatggttgt tcggccgcaa acacggcatc ggcttcctca
     2941 aactgatgga cacggtcgca ccgctcgttc cgctgggtct cgcttcggga cgtatcggca
     3001 acttcatcaa cggcgaactt tggggacgcg ttaccgacat caacgcattt tgggcaatgg
     3061 gcttcccgca ggcgcgttac gaagatgccg aagccgccgc gcacaatccg ctttgggcag
     3121 aatggctgca acaatacggt atgctgccgc gtcatccctc gcagctttat cagtttgcac
     3181 ttgaaggcat ctgcctgttc gccgtcgttt ggctgttctc taaaaaacag cggccgaccg
     3241 gacaagtcgc ctcgctcttc ctcggcggct acggcatatt ccgcttcatt gccgaattcg
     3301 cacgccaacc cgacgactat ctcgggctgc tgaccttggg gctgtcgatg gggcaatggt
     3361 tgagcgtccc gatgattgtt ttgggtatcg tcggctttgt ccggttcggc atgaaaaaac
     3421 agcactgacc gcctgtgaaa aataccgtct gaaacatgga aatccggttt cagacggcat
     3481 ttttacgttt ggttcatgga aacaaacgct tcaattctta ccgcaacacg ccttgtattt
     3541 tctgccgctg ccgcacggac agggatcgtt cctgcctgtt ttttcgcctt ccctgcggac
     3601 ggtttgcggc ttgttgatga ccgcctgcca gtagcggtag atatccgcca atgcataagg
     3661 caactcggat tccagttccg ccagctcgcc ttctgtgaat tgcagacgga tagtgccgtt
     3721 gtcctcttcg tcgtaaatac cgcccaatgc catgatggga taaaacaact cttcaaacgc
     3781 ttcatcatcg acggcttcaa accaatcggt cggcacaata tccaaaccgt aaagataagc
     3841 attgcaccat gtgtaaaaat cgctgccgcc gtcttcgttt tcatacagcc acaaatcggg
     3901 cagtttttta tccgacatcg cggcggttgt ttccatcgcc attgccaaaa ccagccgttc
     3961 gatttcggaa cgttcggcgg cggtaaattg cgattcgtcg cccaacactt cgggcagcca
     4021 gtcgagcggt gtcaatttgt ccggcccgct caacagcgcc gtcataaaac cttgaacctc
     4081 gtcgcaacgc atcgtgttgc cttgttcgct tttggcatcc aacaattcgc tcaaccgccg
     4141 tttggatgct tcggtaaatt tttgggaatc catcatttaa ccttttaaaa tgggtttaat
     4201 acctaattat aatcgcaata tgccgtctga agcctttttc tgcttcagac ggcatcgtct
     4261 gttttcacgc tcaaatcgta ccgctgcctt gcgctttcgg tttggcggca ctaaattcca
     4321 gtttgctcaa ggcggaaagg taggctttgg cggtggcaac caaaacgtcg gtatccgcgc
     4381 cctgaccgtt gacgacgcgg ttgccgcgtg ccagacggac gctggtttcg ccttggcttt
     4441 ccgtaccttg cgtgacggca ttgacggaat aaatctgcaa agtcgcgccg ctttgcgcca
     4501 cgctttcaat cgctttgaaa attgcatcaa ccggaccgga accggttgcg gaagcgcgtt
     4561 tttcttcgcc tttgatgctg aacacgatgt cggcgcgcgg ctcttcgccg gtttcggtgc
     4621 tgattttttg ggaaatgaat ttgtagcttt cggtattcat attgcccatt tcgtccgaca
     4681 ccagcgcgtg caggtcttca tcgaagattt cgcgtttttt gtcggcgagt tctttgaagc
     4741 gtgcaaatgc cgcgttcagt gcctcttcgc tttccaactc gatgcccaaa tccgccagct
     4801 tggttttgaa ggcgttgcgg ccggacaatt tgcccaagct caatcggtta gccgaccaac
     4861 cgaccgattc ggcagacata atctcgtaag tttcgcggtg tttcagcacc ccgtcctgat
     4921 ggatgcccga ttcatgcgaa aaggcattgg caccgacaat ggctttgttg ggctgcacgg
     4981 gatagcccgt aatggtggac accagtttgg acgaaggcac gatttgcgtg gtatcgatgc
     5041 cggtttccaa gccgaacaag tcgtggcgca ctttcaacgc catcacgatt tcttcaaccg
     5101 aagcattgcc tgcacgttcg cccaagccgt tgacagtaca ttccacctga cgcgcaccgc
     5161 cttttaatgc ggcaagcgaa ttggcaaccg ccaagcccaa atcgttgtgg cagtgtgccg
     5221 accaaacgac tttgccgccg ttgggcgttt tggcaatcag ttcgcggaaa aattcttcgg
     5281 ttttgtacgg gatggaatag ccgacggtat cgggaatatt gatggtggtc gcgcccgctt
     5341 caattaccgc gccgcagatt tcggcaagga aatcgatttc cgaacgcaac gcgtcttcgc
     5401 aggaaaattc cacatcgtcg gtgtattcac gagcgatttt caccgctttg accgccgcct
     5461 caatcacctg cttcggcttc atcttcaatt tgtactccat atggatgggg ctggtggcga
     5521 tgaaggtgtg gatgcgtttt ttcggcgcgg gcgcaacggc ctcacccgcc tgacggatgt
     5581 cccgctcgat ggcgcgggac aatgaacaga ccgttgattt ggtaatggtt ttcgcaatcg
     5641 cattgaccgc ctcgaaatcg cccgggctgg cagcggcaaa acccgcttcg atgatgtcca
     5701 cacccaattt ttccagctgg cgggcgacgc ggattttttc ctctttggtc atagcagcgc
     5761 cgggcgactg ctcgccgtcg cgcagggtgg tgtcgaaaat gataacgcgg ttggcctgtg
     5821 tcatttctgt tctctccaga gattcgtttt tttgtaaaaa agcattcaaa tccagcggcc
     5881 tgccttgcga ttcggcaagt gcagtcaatt tttgcagctg cgccaaaggc agcgagccgc
     5941 gcgtgcgcca tttgtagatg gcggcagttg tcgcggcatt ttcgggatcg tgccgtttca
     6001 acgcttcggc aagcgcgttc acgccgccga aataggcgac cagacggtct atatctaatt
     6061 gcatggtagt ccttgtctaa aatcaatctg cccgcagcag aaatatggac aggattatac
     6121 gcattttaga caaaacggca atatcaattt gaaaaaatct caataactca tcatagattt
     6181 tattaatttt atacattttg tctattttat tgcaggccca gcgcaattgt tgacaattgc
     6241 ggcagaacag ggcaaattcg acagcaacaa accaaacgct acaaacgcaa agaacagaaa
     6301 caatgaaata caagagaatc gtatttaaag tcggcacatc ttcgattacc cattcggacg
     6361 gcagcctctc gcgcggcaaa atccaaacca ttacccgcca gcttgccgca ttgcatcatg
     6421 cgggacacga gctggtcttg gtttcatccg gcgcggttgc ggcagggttc ggtgcgctgg
     6481 gtttcaaaaa acgtccggcc aaaatcgccg acaaacaggc ttccgccgcc gtcgggcagg
     6541 ggctgctgat ggaagaatat acggcaaacc tgtcttcaga cggcatcgtg tccgcgcaaa
     6601 tcctgctcaa ccgcaccgac tttgccgaca aacgccgcta ccaaaatgcc ggcggcgcgc
     6661 tttccgtgct gctgcaacgc cgcgcgattc ccattattaa tgaaaacgac acggtttcgg
     6721 ttgaggagtt gaaaatcggc gacaacgaca cattgagtgc gcaagtggcg gcgatgatac
     6781 aggcagacct cttggtgctg ctgaccgaca tagacggtct ttacacgggc aacccgaaca
     6841 gcaatcccga tgccgtacgg ctggacagaa tcgaacacat caaccatgaa atcatcgaaa
     6901 tggcaggcgg cgcgggctcg tcaaacggca cgggcggtat gctgaccaaa atcaaagcgg
     6961 cgacgattgc gaccgagtcc ggcgtaccgg tctatatctg ttcctcgctc aaacccgatg
     7021 cgcttgccga agcggcagat aatcaggcgg acggctcgtt tttcgtcccc cgcgccaaag
     7081 gtttgcggac gcagaagcaa tggctggcgt tctattccga aagccggggc ggcgtttatg
     7141 tggacgaagg tgcggaacac gctttgtccg aacagggaaa gagcctgctg atgtcgggcg
     7201 ttgccggaat cgaagggcat ttttcccgta tggacaccgt aaccgtgtac agcaacgcaa
     7261 ccaaacagcc tttgggcaaa gggcgcgtcc ttttcggctc tgccgccgcc gaagacctgc
     7321 tcaaatcgcg caaagcgaaa ggcgtgttca tccatcggga cgattggatt tccatcacgc
     7381 ccgaaatacg cctgcttctg accgaatttt agaaaaggaa acccatgtca aacacacaaa
     7441 aacagcttgc ccttgccaaa gcggcaaaaa aatccgtcaa cacggcggat acggaagaaa
     7501 aaaaccgcgc gctgcttgct atggcggaca gtctggaagc ggcggcggca gatattttgg
     7561 cggcaaaccg tttggatttg gaagcggcgg caggcaaaat tcccgaaagc atgaccgacc
     7621 gccttttgtt ggacggcaaa cgcatttgcg cgatggcgga cggcatcagg gcggttgccg
     7681 cgctgcccga ccccgtgggc gaaatactgg aaacctcgac tctgcccaac ggcttggaaa
     7741 tcgtcaaaaa gcgtgtggcg atgggcgtta tcggcattat ttacgaaagc cgcccgaacg
     7801 ttacgtccga tgcggcggct ttggcactga aaagcggcag cgcggtcgta ctccgcagcg
     7861 gcaaagatgc attccaatcc gcacgcgcca tcgttgccgc actcaaagcc ggtttggcgc
     7921 aaacccgcat cgaccccgac gcgctccagt tgattgaaga caccgggcgt gagggcagtt
     7981 acgaaatgat gagggcgaaa gattatctag acctgctgat tccgcgcggc ggggcggggc
     8041 tgatacgggc ggtggttgaa aatgccgtcg tgccggttat tgaaacgggg acgggcatcg
     8101 tccacattta tatcgacaag gacgcggatt gggacaaggc gctccgtatc gtgtacaacg
     8161 ccaaaaccag ccgtccgtcc gtgtgcaact cgatggaagt gctgctggtg catgaagaca
     8221 ttgctgccga cttcctgccc aagctcgaac ggctgttggt tcgcgaccgt ataaaagccg
     8281 gactgccgcc cgtccgcttc cgtttggatc cgcaggcggc acggcatatc ggcggcgaag
     8341 cggcgggtgc agacgatttc gataccgagt ttttagacta catcctcgcc gtgaaaaccg
     8401 tcgcttcggt cgaagaggcg gtcgggcaca tcgaaacgca cggcacgcac cattcagacg
     8461 gcatcgttac cgaaaaccgc cacgctgcgg actatttcac gacccatatc gattctgccg
     8521 ccgtgtatgt caacgcgtcc acgcgcttta ccgacggcgg cgaattcggc ttgggttgcg
     8581 aaatgggcat ctccacgcaa aaactccacg cccggggtcc gatgggtttg aaagagctga
     8641 caagctataa atacatcatt cggggcgttg ggcaggtcag ggaataatcc gcattcaaac
     8701 gtccgataaa aatgccgtct gaaacagtct tcagacggca tttccatttg caaaaaaatc
     8761 aagcattgtc caaggggacg agaatcgtac ggttgccgtt gtgttccggc gcggatacga
     8821 tgccttccgc ctccatctgg tcaatcagcc gcgcggcgcg gttgtagccg atacgcaatg
     8881 cgcgctgcac gccagaaatg ctggctttgc gcgttttcaa aacaacggac acggcctcat
     8941 cgtacatcgg atcggtttcg ccgtcgccgc tgcgcccgat gccgggcagc tcttcgctgc
     9001 cgccgccgct caaaatatcg tcaacatagt ccggttcgcc aaactgcttc agatattcga
     9061 ccacgcggtg cacctcttca tccgaggcaa acgcgccgtg aacgcgctgc ggataggcag
     9121 tacccggcgg caggaacagc atatcgccct gaccgagcag gttttccgcg cccatttggt
     9181 cgagaatcgt gcggctgtcg attttgctgg acacttggaa cgcgatacgc gtcgggatgt
     9241 tcgccttaat cagacccgtg atgacatcga cgctggggcg ttgtgtggca aggataagat
     9301 ggatgcctgc cgcgcgggct ttttgggcga ggcgggcaat cagttcctcg attttcttgc
     9361 ctgccgtcat catcaaatcg gcaaactcat cgaccaccac cacgataaac ggcagttttt
     9421 ccaaaggttc gggatcgtcg ggcgtgaggc tgaacgggtt gccgattttc tccccccttg
     9481 ctgcggcttc ggcgattttt tgattgaaac ccgcaagatt gcgcacgccc ataaagctca
     9541 tcaggcggta gcgtttttcc atttcgttaa cacaccagtt cagcgcgttt gccgccagct
     9601 tcatatcggt aacgacaggg gcgagcaggt gcgggatgcc ttcgtaaatg ctcaattcca
     9661 gcattttcgg atcgatcata atcatacgca cgtcttccgg cgcggctttg aaaagcatag
     9721 acagaatcat cgcgttgaca cccaccgatt tgcccgaacc ggtcgtgccg gcaaccagca
     9781 aatgcggtgc tttgcccaag tcggttacga cgggctgtcc ggtgatgtcc tgaccgagcg
     9841 cgagcgtcag cttggatttg gattcggcaa actcgggcga attgaagatt tcgctcaggc
     9901 gtatcatttg gcgtttcggg ttcggaagtt ccaaacccat acaggttttg ccgaggatgg
     9961 tttcgacaac gcggatggaa gccacgccga gcgaacgcgc caagtctttt tccagattta
    10021 gaacggaatt gccgcgcacg ccgacatcgg gttcgatttc ataacgcgta atcacggggc
    10081 cggaataaga atcgacaacc ttgaccttga ctttgaactc cgccaatttt tcttcgatgg
    10141 tgatgctgtt ttccaacagt tcttcttcgg tttgcgtcgc ctcgggattg aacagcggcg
    10201 gcagaagcag gtcggttgtc ggcaggtgtt cggataccgc accggtttct tcagatggga
    10261 acgccccttc atccgcttcc gtatccgata cccggcagga cgggcgttca gacggcacat
    10321 tttcaaacgg acaaaccgcc tgactgtcat gcccgaacgc ttcggtttcc gacagatatt
    10381 gcccgtttga ccgctctgcc gcaccttcac tgccgtcatc tgcaatagcg gcggtttcct
    10441 cctgccaacc tccattcaaa acatcatcgg caagatggtc ggtttcagca atgcggctgc
    10501 gttgcgcctg ctcgaatcct gtcggcggct cataggtacg gttgtagatt tccgatacgg
    10561 gaggcggcgg cggaatatcg attgcgggca tgggaacttt cggcacttcg ggtggttcga
    10621 ccacccaagc atcgggcgca gcaggttctt caatatagat atcggcagct tcatcataaa
    10681 ccggaccgcc gtatcccaca gacgaaacgg tttccgtgaa aacattttcg gtttcagacg
    10741 gcatttcgac atcccgtttc ccgacaaccg tccgactttc cggaatcagg cgcgaaatcc
    10801 gcgcctccgc agaaaccgtt gccttgttcg gttctttcaa attgacggaa acgcggcggc
    10861 aggcatcgcg gacggctgtg ccgtctgaaa cggactgtcc ttgggaaaca tccggggatt
    10921 ttgcctccgc ctgccctgca tgacggcgga acggcgcatt ccgtgcattt tcggctttga
    10981 ctgcagaaaa ggcagacgga tgctgcttct caaaatacgg ctcaaatccg taatccgcag
    11041 actcggaaaa cgcttctttg tccgcatcga acatacggga atacgtgcgt tgaagcacag
    11101 ggtcgtccaa accgataatc tgtagccctt ccatcggggt atcggacacg cggactttgg
    11161 ggaccgctgt ttcgtttttc tcaaatgcat cgatatagcg ttttttcgtt tcccttaaag
    11221 ccgcgctgtt taaagcacgc gttgcttctt ccaaagtgat ttccttaaaa accggacgga
    11281 cgggcgaaat ttcgctttca gacggcatca acccttcagc aatactccgg tcgaatggga
    11341 tgcggcggtt gtcgattacg gcagttgcaa tgtcttccgt atcggcagcc tcttcctccg
    11401 cagcttctgc ttcttccgtt tccgcttctt ccgtcccgtt tccactgtct gaatatccgt
    11461 cggatgcggt cggaacatcc gcacggtttg cagaagcagg cggaacgggc ctgtccggtt
    11521 cggattcgtg cgccagatag tttcggcaga aacggacgac accgacaaac agccaataca
    11581 ctgccgtttt gacggcatgg aaaagcatca gggcaagttc gggaaaatcg ggcataccgt
    11641 ctttgatttc aggcagctcc gcctgttttt cccctttctt ttcctgccac gcagaaacct
    11701 cgcgcatcca ctcgcgttcg gattgtgcgc ggacaaaaaa caagccggca agcgcaagca
    11761 acaaaataac gatcaaaact atccaaaaca tatgccgttt cccgcaagac aaacgattaa
    11821 acgggtttat tttaacgtgt ttccatccca aaaaaaacaa ccggtcggca attctgccga
    11881 aaccgattcg gctttcggtt tgcccccgcc gacagtataa tagccgtcct ttcccttaat
    11941 ctttgcggca ttgccatgat tttccaaaca gtctggtttt cagatatggt actgtcggtt
    12001 tcgtggattg tccttatcct gatactggca gcttccgcgc cgtccgcatt ccgctcgctt
    12061 gcccgctacc gttccgccct tcccctgtgt accgtaattt tttccgcagc atggtgtctg
    12121 aacgcctctg ccggcggcgg acagcttgcc cagatgaact accatctgct cgccgtcaat
    12181 ctggttgcac tgatggtggg cacttccgcc gccttttggc ttgccgcgct gctgatgctg
    12241 ccctactgcc tgctgtttgc cggttctgcc ggcgcatatc cgccgaacgc tttggtgctg
    12301 attctgcccg cgcttgccgt caaccgcctg tcgcgtatgc tggtcaaccg cctgccgccc
    12361 aatattttca tctttatttt cgtcaacggc tttctcgctt ccgccgccgg cattctgttg
    12421 accgggctgg tgctgaccgg tattttggat gctgcaaacg cttttccgtc cgaaatattg
    12481 tggacgaccg ccctgcccgt ctttattctg ctggcgtggg cggaagcctt cctcagcggc
    12541 atttcaactg ccatatttgt tgcactgaag ccccattgga tcaacacttt cgacgacaac
    12601 cgctatctga agtccgaccg cggcatatgg cggtagcctt cagacggcat aggcagacaa
    12661 aaccatgctt tccaacatca tccccctttc catcggtgcg gcactcggtg caacggcgcg
    12721 ttggctgctc aacctcgccg ttcctgccgc catgtctccc gccacgggca acctgtttgc
    12781 caactggacg ggcgcgctgc tcatcggcat ctttgccgaa accatcaatc acccgcaatg
    12841 gaagctgctg ctgattaccg gttttctcgg cagcctgacc acgctttccg gattttcact
    12901 ggaaaccgta accctgctcc aatcgaatcg tcctgcttca gcacttgcca atattttcct
    12961 acacacggca ggttcgctgc tgctgacttg gctcgggctg aagatagggg cggcagtcaa
    13021 ataaccgcaa caaattgcct ccctttgttt tgacataatc tataaattag cttcgcttgt
    13081 ttaaatactg gctgtgcca
//
LOCUS       DAXHPG010000040        12947 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXHPG010000040 DAXHPG010000000
VERSION     DAXHPG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.040"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            109..933
                     /locus_tag="ACFM6F_000858"
     CDS             109..933
                     /locus_tag="ACFM6F_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC6164309.1"
                     /translation="MNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLD
                     HKDKSLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIE
                     VDGQLITLESGEFQVYKQSHSALTALQTEQVQDSEHSGKMVAKRQFRIGDIAGEHTSF
                     DKLPEGGRATYRGTAFGSDDASGKLTYTIDFAAKQGHGKIEHLKSPELNVDLAASDIK
                     PDKKRHAVISGSVLYNQAEKGSYSLGIFGGQAQEVAGSAEVETANGIRHIGLAAKQ"
     gene            1073..1750
                     /gene="tsaB"
                     /locus_tag="ACFM6F_000859"
     CDS             1073..1750
                     /gene="tsaB"
                     /locus_tag="ACFM6F_000859"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC6164310.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETRLFHQEVGSRQSALI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERRARL"
     gene            1747..2187
                     /gene="rimI"
                     /locus_tag="ACFM6F_000860"
     CDS             1747..2187
                     /gene="rimI"
                     /locus_tag="ACFM6F_000860"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC6164311.1"
                     /translation="MNIRRAVYADCAALAALDAVCNPSAWTQRQFESALVSLSEQVFL
                     AEKDGGLAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWFAHLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRATDGQTEDAVLMEKIC"
     gene            2181..2915
                     /locus_tag="ACFM6F_000861"
     CDS             2181..2915
                     /locus_tag="ACFM6F_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6164312.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNPMP
                     SEQAVANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKHALQAGGS"
     gene            2977..3618
                     /gene="pyrE"
                     /locus_tag="ACFM6F_000862"
     CDS             2977..3618
                     /gene="pyrE"
                     /locus_tag="ACFM6F_000862"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC6164313.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            3692..4126
                     /locus_tag="ACFM6F_000863"
     CDS             3692..4126
                     /locus_tag="ACFM6F_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC6164314.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTLHDTPPATAATP
                     AAAPQVAVPPAAPARQEGFNWTIATLFALIVLIMQLSYLVIL"
     gene            4123..5433
                     /gene="argA"
                     /locus_tag="ACFM6F_000864"
     CDS             4123..5433
                     /gene="argA"
                     /locus_tag="ACFM6F_000864"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC6164315.1"
                     /translation="MIVPDLFVAHFREAVPYIRQMCGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASAAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARSMGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKAYRSNGRNSHILVRRLHR"
     gene            5529..7544
                     /locus_tag="ACFM6F_000865"
     CDS             5529..7544
                     /locus_tag="ACFM6F_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC6164316.1"
                     /translation="MKSYPDPYRHFEDLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDTEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKDAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSESEAKPLNLPNDCDVAGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEAELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDMPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPRHIGLQGGSNGGLITAAAFVCEPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRWLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLHETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(7658..8614)
                     /locus_tag="ACFM6F_000866"
     CDS             complement(7658..8614)
                     /locus_tag="ACFM6F_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6164317.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            8850..9815
                     /locus_tag="ACFM6F_000867"
     CDS             8850..9815
                     /locus_tag="ACFM6F_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6164318.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKAAFEKAEPVAAGKE"
     gene            complement(10122..10274)
                     /locus_tag="ACFM6F_000868"
     CDS             complement(10122..10274)
                     /locus_tag="ACFM6F_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164319.1"
                     /translation="MRFVVLASFSGRSCNESVIDFDVSLRLTALSLTLSHRERGRVAV
                     ASLFAA"
     gene            10305..10628
                     /locus_tag="ACFM6F_000869"
     CDS             10305..10628
                     /locus_tag="ACFM6F_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6164320.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            10742..12919
                     /gene="tdfF"
                     /locus_tag="ACFM6F_000870"
     CDS             10742..12919
                     /gene="tdfF"
                     /locus_tag="ACFM6F_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
                     /protein_id="HFC6164321.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKALDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNAEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT         2903 a         4039 c         3581 g         2424 t
ORIGIN      
        1 aggcaattga aaattttttg gcgcattttt atgcgtcaaa tttcgttaac agactatttt
       61 tgcaaaggtc tcatattatc tattctgcgt atgactagga gcaaacctgt gaaccgaact
      121 gccttctgct gcctttcttt gaccgccgcc ctgattctga ccgcctgcag cagcggaggc
      181 ggcggtgtcg ccgccgacat cggcgcgggg cttgccgatg cactaaccgc accgctcgac
      241 cataaagaca aaagtttgca gtctttgacg ctggatcagt ccgtcaggaa aaacgagaaa
      301 ctgaagctgg cggcacaagg tgcggaaaaa acttatggaa acggcgacag cctcaatacg
      361 ggcaaattga agaacgacaa ggtcagccgc ttcgacttta tccgtcaaat cgaagtggac
      421 gggcagctca ttaccttgga gagcggagag ttccaagtgt acaaacaaag ccattccgcc
      481 ttaaccgccc ttcagaccga gcaagtacaa gattcggagc attcagggaa gatggttgcg
      541 aaacgccagt tcagaatcgg cgatatagcg ggtgaacata catcttttga caaacttccc
      601 gaaggcggca gggcgacata tcgcgggacg gcattcggtt cagacgatgc cagtggaaaa
      661 ctgacctaca ccatagattt cgccgccaag cagggacacg gcaaaatcga acatttgaaa
      721 tcgccagaac tcaatgttga cctggccgcc tccgatatca agccggataa aaaacgccat
      781 gccgtcatca gcggttccgt cctttacaac caagccgaga aaggcagtta ctctctaggc
      841 atctttggcg ggcaagccca ggaagttgcc ggcagcgcag aagtggaaac cgcaaacggc
      901 atacgccata tcggtcttgc cgccaagcag taaccattgt gaaaatgccg tccgaacacg
      961 ataatttacc gttcggacgg catttttgcg ccataccgcc ctaggatacg acggcacggc
     1021 aggcaagccg gtttcactat ataatgccgt ctgaaccaac gagaaccaca ccatgcaagc
     1081 cgatttcaac cgccccgtcc tcgccgtcga taccggaact tcctatttgt cgctcgcgct
     1141 gcgcgccgac ggcgaaaccc gtctgttcca tcaggaagtc ggcagccgcc agtccgcact
     1201 gatcctgccg gaaatccgca ccctattccg aaatgcaggc attaccgccg ccgatttggg
     1261 ggcgattgtt tatgcgaaag gccccggcgc gtttacggga ctgcgtatcg gtatcggcgt
     1321 ggcgcaaggt ttggcaacgc cgtttgatac gcctttaatc ggcatcccca cgctcgatgc
     1381 cgccgcctcg ctgccgccgc cgcaaagctg catactcgcc gctgcggacg cgcgtatggg
     1441 cgaagtgttt tatgcgtggt tcgacacgct gaaccgccgc cgtttgagcg attatcaggt
     1501 cgggcgggcg gcagacatcc ggctgccgga gggatgtgcc ttttcagacg gcataggcag
     1561 cgcgttcgcg ttggaagaaa cgccgccgtt ttcaggcaaa cccgatatgc cgaccgccgc
     1621 cgacttcctc aatctggcgg ctgaaggcgg ttttcccgcc gtcgctgcgg cacacgccga
     1681 gctgctctac gtccgcaaca aaatcgccct gactgccaaa gaacaggcgg aacggagggc
     1741 gcgcctgtga acatccgccg cgccgtttat gccgattgcg cggcactcgc cgcactcgat
     1801 gccgtctgca acccgtccgc gtggacgcaa cgccaatttg agtctgcatt ggtttcgctg
     1861 tccgaacagg ttttcctcgc ggaaaaagac ggcgggttgg ccgcctttat cgtttggcag
     1921 aacctgcccg acgaatccga actgcacctg attgccaccg cgcccgaatg ccgccgccaa
     1981 ggaattgcgt ccgccctgct cgaatattgg ttcgcacatc tgcccgaagg cacgcaacgc
     2041 ctgctgctcg aagtccgcgc aggcaacacc gccgcgcagg cactgtacgc ggcgcacggc
     2101 ttcagcattg cgggcaggcg gaaaaactat taccgcgcca ccgacgggca aaccgaagat
     2161 gccgtcttaa tggagaaaat atgttaagcg cgcgctacct ccacctgcac gaagctttgg
     2221 gtttgggtcc gatgtggctg aaacaggccg ctgccgtcct gccgcccaaa aacacacccg
     2281 caaccccggc acaggcacgt cctcaaaccg tccgcgccgc cccgatccgc ccttcccaac
     2341 cccataacgg tcaggcgcgg ctcgaaacga tgaaagcgtt ggaaaccgcc gccgtaccta
     2401 cgcgcaaacc cgcgcctgaa accgaaacgc ctctgcccgg cctttcagac ggcatcgccc
     2461 ccgttcccgc cgcttcgggc atcaccaagc ttgccgtcgt cagcctttgc ccaccgatcg
     2521 aggatgcggt ttacgggcaa ctgttccacg gcaaagcggg tgtcctgctt gacaacatac
     2581 tcaaagccgt aggactggat gccgcttatg tacacaagac cagctgggtg aaaaccgccg
     2641 ccgtcggcaa cccgatgccg tctgaacagg ccgtcgcgaa tgcgctgggt caaatcgcca
     2701 gggaactcga cggctgccgt gcccccgccg tccttttcct cgggcaggct tttgtccagc
     2761 ctgaacggca aacgatgatt gaaactttgt gcggcagccg tcccttcttc atcatcgacc
     2821 atcccgctcg gcttttacgc caacccgaac ttaaagcccg aaactggcag gtgttgaaac
     2881 agttgaaaca cgccttgcag gcaggcggca gttgaagcag gccgcacggg gcggtagaat
     2941 cgcaactgcg tcccaatatc tgacagaaag cacaaaatga ccgatttccg ccaagatttc
     3001 ctcaaattct ccctcgccca aaatgttttg aaattcggcg aattcaccac caaagccgga
     3061 cggcggtcgc cctatttctt caatgccggc ctctttaacg acggcttgtc cacgctgcaa
     3121 ctggcaaaat tctatgcaca atccatcatt gaaagcggca tccgattcga tatgctgttc
     3181 ggtcccgcct acaaaggtat tattttggcg gcggcaaccg cgatgatgct ggcggaaaaa
     3241 ggcgtgaacg tcccgtttgc ctacaaccgc aaagaagcca aagaccacgg cgaaggcggc
     3301 gtgttggtcg gcgcgccgct taaagggcgc gtgctgatta tcgacgacgt gatttccgcc
     3361 ggcacatccg tacgcgaatc gatcaaactg attgaagcgg agggtgcaac ccccgccggt
     3421 gtcgccatcg cgctcgaccg catggaaaaa ggcacgggtg aattgagcgc ggttcaggaa
     3481 gtggaaaaac aatacggtct gcccgtcgcc cccatcgcca gcctgaacga tttgtttatt
     3541 ctgttgcaaa acaaccccga attcggacag ttcctcgaac ccgtccgagc ctaccgtcgg
     3601 cagtacggcg tagaataaaa acaaagcata tgccgtccga accgccttac gcctcagacg
     3661 gcatcaaacc tgacacacac gaggaaatac catgcccgcc tgtttctgcc cccactgcaa
     3721 aacccgtctc tgggtcaaag aaacccaact caatgtcgcc caaggcttcg tcgtctgcca
     3781 aaaatgcgaa ggactgttta aagccaaaga ccatctggca agcacgaaag aacccatatt
     3841 caacgatttg cccgaggctg tttcggatgt caaactcgtt caccgcatcg gcacgcgcgc
     3901 cattggcaag aaacagattt cccgcgacga aatcgccggc atcctcaacg gcggtacaac
     3961 cctgcacgat acgccgcccg caaccgccgc cacccctgcc gccgcaccgc aggttgccgt
     4021 accgcccgcc gcgcctgccc gtcaggaagg gttcaactgg acgattgcaa ccctgtttgc
     4081 ccttatcgtc ctcattatgc agctttccta cctcgtcatc ctatgatcgt gcccgacctc
     4141 tttgtcgccc acttccgcga agccgtcccc tacatccgcc aaatgtgcgg caaaacgctg
     4201 gtcgccggca tagacgaccg cctgctcgaa ggtgatacct taaacaagct cgccgccgac
     4261 atcgggctgt tgtcgcaact gggcatcagg ctcgtcctca tccacggcgc gcgccacttc
     4321 ctcgaccgcc acgccgccgc gcaaggccgc acgccgcatt attgccgggg cttgcgcgtt
     4381 accgacgaaa cctcgctcga acaggcgcag cagtttgccg gcaccgtccg cagccgtttt
     4441 gaagccgcat tgtgcggcag cgtttccggg ttcgcgcgcg cgccttccgt cccgctcgta
     4501 tcgggcaact tcctgaccgc ccgtccgata ggtgtgattg acggaaccga tatggaatac
     4561 gcgggcgtta tccgcaaaac cgacaccgcc gccctccgtt tccaactcga cgcgggcaat
     4621 atcgtctggc tgccgccgct cggacattcc tacagcggca agaccttcta tctcgatatg
     4681 cttcaaaccg ccgcctccgc cgccgtctcg cttcaggccg aaaaactcgt ttacctgacc
     4741 ctttcagacg gcatttcccg ccccgacggc acgctcgccg aaaccctctc ggcacaggaa
     4801 gcgcaatcgc tggcggaaca cgccggcggc gaaacgcgac ggctgatttc gtccgccgtt
     4861 gccgcgctcg aaggcggcgt gcatcgcgtc caaatcctca acggagccgc cgacggcagc
     4921 ctgctgcaag aactcttcac ccgcaacggc atcggcacgt ccattgccaa agaagccttc
     4981 gtctccatcc ggcaggcgca cagcggcgac atcccgcaca ttgccgccct catccgcccg
     5041 ctggaagaac agggcatcct gctgcaccgc agccgcgaat acctcgaaaa ccacatttcc
     5101 gaattttcca tcctcgaaca cgacggcaac ctgtacggtt gcgccgccct gaaaaccttt
     5161 gccgaagccg attgcggcga aatcgcctgc cttgccgtct cgccgcaggc acaggacggc
     5221 ggctacggcg aacgcctgct tgcccacatt atcgataagg cgcgcagtat gggcataagc
     5281 aggctgttcg ccctctccac aaataccggc gaatggtttg ccgaacgcgg ctttcagacg
     5341 gcatcggaag acgagttgcc cgaaacgcgg cgcaaagcct accgcagcaa cggacggaac
     5401 tcgcatattc tggtacgtcg cctgcaccgc tgaccgcaac ggaaagccgc cgcagaaatg
     5461 ccgtctgaac cccgtttcag acggcatttc cccgattata aagacctgcc caaccctcaa
     5521 ggaccccgat gaaatcctac cccgacccct accgccattt tgaagacctc gattccgccg
     5581 aaacgcaaaa cttcgctgct gaagcgaatg ccgaaacgcg cgcgcgtttt ttaaacaacg
     5641 acaaggcacg cgcattgtct gacggcattt tggcgcagtt gcaggacacg cggcaaattc
     5701 cgttttgtca ggaacaccgc gcgcggatgt accatttcca tcaggacacg gaatatccga
     5761 agggcgtgta ccgcgtgtgt acggcggcga cttaccgttc cggctatcct gagtggaaaa
     5821 tcctgttttc ggtggcggat ttcgacgaat tgctcggcga cgatgtgtat ttgggcggcg
     5881 tgtcgcactt ggtggaacag cccaaccgcg cgttgttaac actgagcaaa tcgggcggcg
     5941 atacggcgta cacgctggaa gtggatttgg aagcagggga attggtagaa ggcggttttc
     6001 actttccggc aggtaaaaac catgtgtcgt ggcgcgatga aaacagcgta tgggtgtgtc
     6061 cggcttggga cgaacgccag ttgaccgaat cgggctatcc gcgcgaggtg tggctggtgg
     6121 aacgcggcaa aagtttcgag gaaagcctgc ctgtgtatca aatcgataaa gatgcaatga
     6181 tggtgaacgc gtggcgttat ctcgatccgc agggttcgcc gattgatttg attgaagcgt
     6241 cggacggttt ttacaccaaa acctatttgc aggtgtcgtc ggaaagcgag gcgaaaccgt
     6301 taaacctgcc caacgattgc gacgtggccg gctatctggc cggacatctt ttgctgacgt
     6361 tacgtaaaga ctggaaccgc gcgaaccaaa gctatccgag cggcgcattg gtggcggtga
     6421 agctgaatcg gggcgaactc ggggcggcac agcttttgtt tgcgcccgat gaaacgcagg
     6481 cattggaaag cgtggaaacg accaagcgtt ttgtggtggc gagcctgctg gaaaacgtac
     6541 aaggccgtct gaaagcgtgg cggtttgccg atggcaaatg gcaggaagct gagttgccgc
     6601 gcctgccttc gggcgcgttg gaaatgaccg atcagccttg gggcggcgac gtggtttacc
     6661 ttgccgccag cgatttcacc acgccgctga cgctgtttgc gctggatttg aacgtgatgg
     6721 aactgaccgt catgcgccgc cagccgcagc agtttgattc agacggcatt aacgtgcagc
     6781 agttttggac gacttcggct gacggcgagc gcattcctta tttccacgtc ggcaaaaacg
     6841 ccgcgcccga catgccgacg ctggtctatg cctacggcgg tttcggcatt cccgaattgc
     6901 cgcattatct gggcagcatt ggcaaatatt ggctggaaga gggcaatgcc tttgtattgg
     6961 cgaacatccg cggcggcggc gagttcggcc cgcgctggca tcaggcggcg cagggcatca
     7021 gcaaacataa aagcgttgat gatttattgg cagtcgtgcg cgatttgtcc gaacgcggca
     7081 tgagttcgcc cagacacatc ggtttgcagg gcggcagcaa cggcggcctg attaccgccg
     7141 ctgccttcgt gtgcgaaccg caaagcatcg gcgcgctggt gtgcgaagta ccgctgaccg
     7201 acatgatccg ttatccgcta ttgtccgccg gttcaagttg gacggacgaa tacggcaatc
     7261 cgcaaaaata cgaagtctgc aaacgctggc tgggcgaatt gtcgccgtat cacaatcttt
     7321 cagacggcat cgattatccg cctgcgctca ttaccaccag cctcagcgac gaccgcgtcc
     7381 atcctgccca cgcgctcaag ttttacgcca aactgcatga aacctcggcg caatcttggc
     7441 tctactcgcc tgacggcggc ggccataccg gcaacggcac ccaacgcgaa tccgccgacg
     7501 aactcgcctg cgtcttgctg tttttgaaag agtttttggg ctaagggcgg ggagcggcac
     7561 tgccgccgcg aatgaaaaaa ggtcgtctga aactgctttt tcagacgacc ttttttaatg
     7621 gttgtttcaa atcaaaatat ctatgccgcc ggccccatca gcacttcttc acatccgaag
     7681 gcaaaaatcc gtaatgccgt ctgaacgctt cgttgaaccg tcccgcgtgg cggtagccgc
     7741 aaaagtgcat ggcggcttgg acggtgctgc cggattcgat gagggcgagc gcgtgttcca
     7801 gccgcaggcg gcgcaggcat ccggcgacgg tttcaccggt ttgcgctttg aaatagcgtt
     7861 tcaggtagca ttcgttcagt ccgacgcggc gggcgatttc ggcgatggtc agcggacggg
     7921 cgaattcgtg ttgcaggatg tcggcggctt cgtctatgcg ccggcggcgg taaccgttgt
     7981 cgtggcggcg gaaggtgaag cgcaataatc gggcggagag ttccagcgcg gcggcttcgt
     8041 cggcaagcag gccgaagccg tccgattcga acgggcgttg cagcagtggg caggccgccg
     8101 ccgtcagtgc cgctgcgttt tgcgccagcc gttgcagggc gaatcggcct attgtttgcg
     8161 gcgaaaacag gcgttcgtcc agcaagcctt cgtcgtgcca gcggcgcagt ttttccagcg
     8221 aaaaatccaa atgcagcgcg cacatgccgc tgttgtcggg cagcagggtt tcggatacgt
     8281 ccgccaactc gccgcgtacc agccagattt cgccggcaga cgggcggtat tccctgccgc
     8341 ccatttgtaa ccggttctgc cccgacacca tgacgaacaa ggcgcagttg tggctgaaat
     8401 tgtggatttc ggtgggaaac gcgcccgttc cgccgccgcg catccgcgac aaggtgatgc
     8461 ccgagtcgaa gcggttgatg cacatttcca gatgcaaacc gggctgtttt gcctgcgcaa
     8521 tgagcgcgct gtcggaacag ccgtccaacg cccagccgga tttatcggag cggacatagg
     8581 tttggtactg gcggtagatg gcggcggtgt tcatgattgg ataggaacga gttgtctaac
     8641 aaatgaatta aataggaatt attaccaata atcaagcgca gggattggtt gaaacggaaa
     8701 aggtcgtctg aaagggtgtt tcagacgacc ttttccgtat cgggaatttg ttttgccgta
     8761 tcgggaattt tgcgttttgc ggcgtggttt ctgcaggttg tttgcttaat aataaacatt
     8821 cttattcgta tgcaaaggaa ccgcacaccg tgaaaccgcg tttttattgg gcagcctgcg
     8881 ccgtcctgct gaccgcctgt tcgcccgaac ctgccgccga aaaaactgta tccgccgcat
     8941 ccgcatctgc cgccacactg accgtgccga ccgcgcgggg cgatgccgtt gtgccgaaga
     9001 atcccgaacg cgtcgccgtg tacgactggg cggcgttgga tacgctgacc gaattgggcg
     9061 tgaatgtggg cgcaaccacc gcgccggtgc gcgtggatta tttgcagcct gcatttgaca
     9121 aggcggcaac ggtggggacg ctgttcgagc ccgattacga agccctgcac cgctacaatc
     9181 ctcagcttgt cattaccggc gggccgggcg cggaagcgta tgaacagttg gcgaaaaacg
     9241 cgaccaccat agatctgacg gtggacaacg gcaatatccg caccagcggc gaaaagcaga
     9301 tggagacctt ggcgcggatt ttcggcaagg aagcgcgcgc ggcggaattg aaggcgcaga
     9361 ttgacgcgct gttcgcccaa acgcgcgaag ccgccaaagg caaaggacgc gggctggtgc
     9421 tgtcggttac gggcaacaag gtgtccgcct tcggcacgca gtcgcggttg gcaagttgga
     9481 tacacggcga catcggccta ccgcctgtag acgaatcttt acgcaacgag gggcacgggc
     9541 agcctgtttc cttcgaatac atcaaagaga aaaaccccga ttggattttc atcatcgacc
     9601 gtaccgccgc catcgggcag gaagggccgg cggctgtcga agtattggat aacgcgctgg
     9661 tacgcggcac gaacgcttgg aagcgcaagc aaatcatcgt catgcctgcc gcgaactaca
     9721 ttgtcgcggg cggctcgcgg cagttgattc aggcggcgga gcagttgaag gcggcgtttg
     9781 aaaaggcaga acccgttgcg gcggggaaag agtagggagt cgtctgaaaa cggagcttcc
     9841 gaaggaagcg gggggtttct gcgaagctaa agtgcggttt caacgaattg aaaagcagcc
     9901 tgtattttga aaataccgct caagcaaacc tacggtttgc cgccctctcc ctagccctct
     9961 cccacaggga gaggggattg ggttgcaggc tgcctttaag gtttaggcaa atttttaact
    10021 tcgttgaagc tccgttttca gacgaccttt tggaacacca caggcacacg catcgaacgg
    10081 ctgaatcaaa gattcagacc gatggcagtc cgcacccgag tttatgcggc aaacagcgag
    10141 gctacggcaa cccgccccct ctccctgtgg gagagggtta gggagagggc ggtaagccgc
    10201 aggcttacat caaagtcgat aacgctttcg ttacaactcc gcccactgaa cgaagccaaa
    10261 acgacaaacc gcatcgtaaa ccacccaacc cataggagaa ccccatgcaa aacgaaacca
    10321 tcaacctgaa acagcacctt gccgccatca aagaatactg gcagcccgaa atcatcaacc
    10381 gccacgggtt ccaattccgt ttggtcaaac ttttgggcga ttacggctgg catacgcacg
    10441 gatacagcga caaagtgctg tttgccgtgg agggcgacat ggcggtggac ttcgccgacg
    10501 gcggcagcat gacgatacgc gagggcgaga tggcggtcgt gccgaagtcg gtgtcgcacc
    10561 gcccgcgttc ggaaaacggc tgctcggtgg tgctgattga gttgtccgac ccgtccgagg
    10621 ccgtctgaaa acgaagtttc cgaaggaagc tgagtttctg cgaagctaaa agcagcctgc
    10681 accttcaatc aatatgccga aaatacaacc ccaccgcaca ccaacacaca aaggaaatcc
    10741 catgacacgc ttcaaatatt ccctgctgtt tgccgccctg ttgcccgtgt acgcgcaggc
    10801 cgatgtttct gtttcagacg accccaaacc gcaggaaagc actgaattgc cgaccatcac
    10861 cgttaccgcc gaccgcaccg cgagttccaa cgacggctac actgtttccg gcacgcacac
    10921 cccgctcggg ctgcccatga ccctgcgcga aatcccgcag agcgtcagcg tcatcacatc
    10981 gcaacaaatg cgcgaccaaa acatcaaagc gctcgaccgc gccctgttgc aggcgaccgg
    11041 caccagccgc cagatttacg gctccgaccg cgcgggctac aactacctgt tcgcgcgcgg
    11101 cagccgcatc gccaactacc aaatcaacgg catccccgtt gccgacgcgc tggccgatac
    11161 gggcaatgcc aacaccgccg cctatgagcg cgtagaagtc gtgcgcggcg tggcggggct
    11221 gctggacggc acgggcgagc cttccgccac cgtcaatctg gtgcgcaaac gcccgacccg
    11281 caagccattg tttgaagtcc gcgccgaagc gggcaaccgc aaacatttcg ggctgggcgc
    11341 ggacgtatcg ggcagcctga atgccgaagg cacgctgcgc ggccgcctgg tttccacctt
    11401 cggacgcggc gactcgtggc ggcagcgcga acgcagccgc gatgccgaac tctacggcat
    11461 tttggaatac gacatcgcac cgcaaacccg cgtccacgca ggcatggact accagcaggc
    11521 gaaagaaacc gccgacgcgc cgctcagcta cgccgtgtac gacagccaag gttatgccac
    11581 cgccttcggc ccgaaagaca accccgccac aaattgggcg aacagccgcc accgtgcgct
    11641 caacctgttc gccggcatcg aacaccgctt caaccaagac tggaaactca aagccgaata
    11701 cgactacacc cgcagccgct tccgccagcc ctacggcgta gcaggcgtgc tttccatcga
    11761 ccacaacacc gccgccaccg acctgattcc cggttattgg cacgccgacc cgcgcaccca
    11821 cagcgccagc gtgtcattga tcggcaaata ccgcctgttc ggccgcgaac acgatttaat
    11881 cgcgggtatc aacggttaca aatacgccag caacaaatac ggcgaacgca gcatcatccc
    11941 caacgccatt cccaacgcct acgaattttc ccgcacgggc gcctatccgc agccatcatc
    12001 gtttgcccaa accatcccgc aatacggcac caggcggcaa atcggcggct atctcgccac
    12061 ccgtttccgc gccgccgaca acctttcgtt gatactcggc ggcagataca gccgttaccg
    12121 caccggcagc tacgacagcc gcacacaagg catgacctat gtgtccgcca accgtttcac
    12181 cccctacaca ggcatcgtgt tcgacctgac cggcaacctg tcgctttacg gctcgtacag
    12241 cagcctgttc gtcccgcaat cgcaaaaaga cgaacacggc agctacctga aacccgtaac
    12301 cggcaacaat ctggaagccg gcatcaaagg cgaatggctt gaaggccgtc tgaacgcatc
    12361 cgccgccgtg taccgcgccc gtaaaaacaa cctcgccacc gcagcaggac gcgacccgag
    12421 cggcaacacc tactaccgcg ccgccaacca agccaaaacc cacggctggg aaatcgaagt
    12481 cggcggccgc atcacgcccg aatggcagat acaggcaggt tacagccaaa gcaaaacccg
    12541 cgaccaagac ggcagccgcc tgaaccccga cagcgtaccc gaacgcagct tcaaactctt
    12601 cactgcctac cactttgccc ccgaagcccc cagcggctgg accatcggcg caggcgtgcg
    12661 ctggcagagc gaaacccaca ccgaccctgc cacgctccgc atccccaacc ccgccgccaa
    12721 agcccgcgcc gccgacaaca gccgccaaaa agcctacgcc gtcgccgaca tcatggcgcg
    12781 ttaccgcttc aatccgcgcg ccgaactgtc gctgaacgtg gacaacctgt tcaacaaaca
    12841 ctaccgcacc cagcctgacc gccacagtta cggcgcactg cggacagtga acgcggcgtt
    12901 tacctatcgg tttaaataag gtcgtctgaa aacggagttt ctgcgaa
//
LOCUS       DAXHPG010000041        12924 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXHPG010000041 DAXHPG010000000
VERSION     DAXHPG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12924
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.041"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..127)
                     /locus_tag="ACFM6F_000871"
     CDS             complement(<1..127)
                     /locus_tag="ACFM6F_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6164322.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWR"
     gene            complement(202..1545)
                     /gene="argG"
                     /locus_tag="ACFM6F_000872"
     CDS             complement(202..1545)
                     /gene="argG"
                     /locus_tag="ACFM6F_000872"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC6164323.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMDVAFWDENVEIKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRVKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            1670..2470
                     /locus_tag="ACFM6F_000873"
     CDS             1670..2470
                     /locus_tag="ACFM6F_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC6164324.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFVFFKSLLESKGLKLNQVQYLP
                     DEPDLLVKQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHAQAAGFIEG
                     VTLKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTQRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            2510..3496
                     /locus_tag="ACFM6F_000874"
     CDS             2510..3496
                     /locus_tag="ACFM6F_000874"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC6164325.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            3545..4072
                     /locus_tag="ACFM6F_000875"
                     /pseudo
     CDS             3545..4072
                     /locus_tag="ACFM6F_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684692.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            4152..4414
                     /locus_tag="ACFM6F_000876"
                     /pseudo
     CDS             4152..4414
                     /locus_tag="ACFM6F_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4452..4826)
                     /locus_tag="ACFM6F_000877"
     CDS             complement(4452..4826)
                     /locus_tag="ACFM6F_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6164326.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEDWLELAIKDTW"
     gene            complement(4823..4951)
                     /locus_tag="ACFM6F_000878"
     CDS             complement(4823..4951)
                     /locus_tag="ACFM6F_000878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164327.1"
                     /translation="MPQTWGEAIQFRIKKQIENELAPPNWSTQFPNGSIYDPKVTK"
     gene            complement(4999..5355)
                     /locus_tag="ACFM6F_000879"
     CDS             complement(4999..5355)
                     /locus_tag="ACFM6F_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC6164328.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWGLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     ALEYAIKNEKTVIFHF"
     gene            complement(5360..5464)
                     /locus_tag="ACFM6F_000880"
     CDS             complement(5360..5464)
                     /locus_tag="ACFM6F_000880"
                     /inference="COORDINATES: protein motif:HMM:NF018014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164329.1"
                     /translation="MDDIRGIIQGAVNPLIYKVSKQKIIYNNKLISGT"
     gene            complement(5498..5842)
                     /locus_tag="ACFM6F_000881"
     CDS             complement(5498..5842)
                     /locus_tag="ACFM6F_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164330.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(6001..6402)
                     /locus_tag="ACFM6F_000882"
     CDS             complement(6001..6402)
                     /locus_tag="ACFM6F_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164331.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(6406..6858)
                     /locus_tag="ACFM6F_000883"
     CDS             complement(6406..6858)
                     /locus_tag="ACFM6F_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164332.1"
                     /translation="MQTKALGTVDEIGDTVQQVGKQASGQKTSGGNPAIDSDPYSPSS
                     VAARIEAGKARSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDFNSLNVRNVQ
                     TRPNGTITGNLPDGRAVNARNDSSGGEPTLEITISNNRKIKIRYGNTR"
     gene            complement(6853..7146)
                     /locus_tag="ACFM6F_000884"
                     /pseudo
     CDS             complement(6853..7146)
                     /locus_tag="ACFM6F_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(7216..7533)
                     /locus_tag="ACFM6F_000885"
     CDS             complement(7216..7533)
                     /locus_tag="ACFM6F_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164333.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(7530..7790)
                     /locus_tag="ACFM6F_000886"
     CDS             complement(7530..7790)
                     /locus_tag="ACFM6F_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6164334.1"
                     /translation="MRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDGVKIDIRPN
                     GEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
     gene            complement(7788..8132)
                     /locus_tag="ACFM6F_000887"
                     /pseudo
     CDS             complement(7788..8132)
                     /locus_tag="ACFM6F_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(8558..8902)
                     /locus_tag="ACFM6F_000888"
     CDS             complement(8558..8902)
                     /locus_tag="ACFM6F_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164335.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(8940..9284)
                     /locus_tag="ACFM6F_000889"
     CDS             complement(8940..9284)
                     /locus_tag="ACFM6F_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164336.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(9287..9571)
                     /locus_tag="ACFM6F_000890"
     CDS             complement(9287..9571)
                     /locus_tag="ACFM6F_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164337.1"
                     /translation="MAGGNKSLKIGTQSVEKSTGRTIPNNLKEQLAMEEVKANPQGKT
                     PARIPPMSDTKNGWLAKDGWVKRVQNVNKIEIHYIENSRTGEKTDFKFKD"
     gene            complement(9545..9889)
                     /locus_tag="ACFM6F_000891"
                     /pseudo
     CDS             complement(9545..9889)
                     /locus_tag="ACFM6F_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(9973..10275)
                     /gene="imm40"
                     /locus_tag="ACFM6F_000892"
     CDS             complement(9973..10275)
                     /gene="imm40"
                     /locus_tag="ACFM6F_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm40 family immunity protein"
                     /protein_id="HFC6164338.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(10301..10567)
                     /locus_tag="ACFM6F_000893"
                     /pseudo
     CDS             complement(10301..10567)
                     /locus_tag="ACFM6F_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10588..10794)
                     /locus_tag="ACFM6F_000894"
     CDS             complement(10588..10794)
                     /locus_tag="ACFM6F_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC6164339.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(10760..11200)
                     /locus_tag="ACFM6F_000895"
     CDS             complement(10760..11200)
                     /locus_tag="ACFM6F_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB"
                     /protein_id="HFC6164340.1"
                     /translation="MAGGNKPPKPSTQQHPTHSDNNIGLPASYVKPDTSISPTGTIQD
                     RIRWTKSKFPTEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSDKILGYQTEHR
                     RVRYDINNNIYVLANPKTFKIKTMFKPNLGKKYYDGEFKKDMGN"
     gene            complement(11180..11509)
                     /locus_tag="ACFM6F_000896"
                     /pseudo
     CDS             complement(11180..11509)
                     /locus_tag="ACFM6F_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(11615..11986)
                     /locus_tag="ACFM6F_000897"
     CDS             complement(11615..11986)
                     /locus_tag="ACFM6F_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6164341.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(11983..12338)
                     /locus_tag="ACFM6F_000898"
                     /pseudo
     CDS             complement(11983..12338)
                     /locus_tag="ACFM6F_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12366..12572)
                     /locus_tag="ACFM6F_000899"
     CDS             complement(12366..12572)
                     /locus_tag="ACFM6F_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164342.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(12582..>12924)
                     /locus_tag="ACFM6F_000900"
     CDS             complement(12582..>12924)
                     /locus_tag="ACFM6F_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164343.1"
                     /translation="PPKSITSNSKADASTQPSLQAQLIGEQISSGHAYNKHVIRQQEF
                     TDLNINSPADFARHIENIVSHPTNMKELPRGRTAYWDDKTGTIVIRDKNSDDGGTAFR
                     PTSGKKYYDDL"
BASE COUNT         3490 a         2737 c         2553 g         4144 t
ORIGIN      
        1 cccgccagcc ttcgggggtt tgtttgttga ggaacgcgct gaaattgacg gggttgacct
       61 ttgccgcgcc gaaaaacagg ctggacaaca ggctttcctg ataaatgacg actttgtatt
      121 ctttcatagg attcctttta acataaggga tcatcttttc agacgacccc ttaagttgct
      181 aatgatgtaa aacagcaaac cttattgctt attgcccaac tgcggcaata ccgaaccttc
      241 gcccagcgag agcaaaccgc tttgcgagta gatacccagt ttgacgcggg tgtcggtgat
      301 gtcgaggttg cgcatcgtga gctgtccgat gcggtcgagc ggagtgaacg cagcgtcttc
      361 gactttttcc atactcaggc gttcaggttg gtaggtcagg ttgggcgatt cggtgttcag
      421 aattgagtag tcgttgccgc gccgcagttc gagggtaact tcgccggtaa cggctttagc
      481 gacccagcgt tgggcggttt cgcgcaacat cagggcttgg ctgtcgaacc agcggccttg
      541 gtagagcaaa cgtccgaggc gcaggccgtt gatgcggtat tgttcgatgg tgtcctcgtt
      601 gtggatgccg gttaccaagc gttcgtaggc gatgtggaac aacgccattc ccggggcttc
      661 gtagatgccg cgcgatttgg cttcgatgat gcggttttcg atttggtcgc tcatacccag
      721 accgtggcgg ccgccgatgc ggttggcttc gaggaagagt tcgacgggat cagcgtattc
      781 tttgccgttc agtgcaaccg gcacgccttc ttcaaagcgc acgctgactt cttcgggttt
      841 gatttcgacg ttttcgtccc agaaggcaac gtccataata ggtttgacga ttttgatgcc
      901 gctgttgagg aattctaaat ctttggcttc gtgggtcgcg ccgagcatat tggaatcggt
      961 ggagtaggct ttttcaaccg acattttgta gttgaagccg ttggcaatca gaaattcgct
     1021 catttcgtgg cggccgccga gttcgtcgat aaattgctgg tcgagccagg gtttgtagat
     1081 tttcagcgcg ggattggtga gcaaaccgta gcggtagaaa cgctcgatgt cgttgccttt
     1141 gtaggtgctg ccgtcgcccc agatgtttac gtcgtcttct ttcattgcgg aaacaagcat
     1201 cgtgccggtt acggcgcggc ccagaggcgt ggtgttgaaa taggcgatgc cgccggtgga
     1261 aacgtggaac gcgccgcatt ggatggcggc gatgccttcg tgtgccaact gcgcgcggca
     1321 gtcgattaag cgggcgtttt ccgcaccgta ttccatcgct tttttgggaa tggcgttgta
     1381 gtcgtcttca tcgggctgac cgaggttggc agtgtaggca taaggcagtg cgcctttgag
     1441 tttcatccac aacagcgcgg cggaggtatc aagtccgccg gagaaggcga tgccgacttt
     1501 ctgaccgacg gggaggtttt gtaaaatagt atggttttgg ttcatgaaaa aatcctcggt
     1561 agcttgaaaa atcaaagcca ttatcttata aataggaaat attgtcagcc atcatgccgt
     1621 ctgaacgggt attccgccgt ttcaggcggt atttaaaacc gaaaacatta tgaacgcgtt
     1681 caaccttatc atcatcggcg acgaaatctt acacggcagc cgccaagaca agcatttcgt
     1741 ctttttcaaa tccctgctcg aatccaaagg gctgaagctc aatcaggtgc aatacctgcc
     1801 cgacgaaccc gatttgttgg tcaaacaact gcgccgcagc ttttcagacg gcatgccaac
     1861 tttcgttacc ggcggtatcg gcgccacgcc cgacgaccac acccgccaag ccgcagccgc
     1921 tgctttggat ttgcccgtcg tccgccacgc gcaagccgcc gggtttatcg aaggcgtgac
     1981 ccttaaacgc ggtgagccgc tcgattcgcc cgaacacgcc caacgcctga agatggcgga
     2041 ttttccccaa ggcgcggaat tggtgcctaa tccgtttaat aatatcgccg gattttccat
     2101 tcgcgagcat tatttcttcc ctggttttcc tgtgatggcg cacccgatgg cggaatgggt
     2161 attggaaact tattatgccg accgcttcaa ccaaacccaa cgcggcagcc gcagcgtgta
     2221 tgtgttcgag cagcccgaat cgcgcattac gccgcttatg gagcatatcg agcaaactta
     2281 tcccggcgtg cgttcgtaca gcctgcccag cgttggttgg acgcattcag acggcacgca
     2341 ggtcaaaccg cacattgagt tcggcatcaa agccgaagga gaagccgtca accttttgga
     2401 cgcggcgtgg gcggaagtgt tgcacagctt ggacggattg ggagcagagc tgaaaaatcg
     2461 ggtaaactga aaaccgtttg cccaaatcgg acgatattta aggaaaacca tgcaataccg
     2521 aatcagaaga gaaaacgaag cacccgaagc aaaaaatacg ggggaaacct tatgggagcg
     2581 cgacatcatg cgcgaagtgc tgctgtccgc ctatcaggac aggcgcaggg agcggatgtg
     2641 gaaaaacatc tggcgcgccg tcagcaccct gattctggtt gccctgattg caggcatttt
     2701 ccgaaaagac gaagccgcat tgcagttggc gggcaacacg ccgcataccg cagtcgtcaa
     2761 tctgtacggc gaaatcggca acggtgtaga agaccaggtc aaaaaactca aagacggtat
     2821 ggaggccgcc tacaaaaacc cgcaggcaaa agccatcgtc atccgcgcca acagccccgg
     2881 cggttcgccc gtcgtctcca acaccgcttt tgaagaaata cgccgtctga aggcgcagca
     2941 ccctggcatt cccgtttatc tcgtggcgga agatatgtgc gcgtccggct gctactacat
     3001 cgcggcggcg gcggacaaaa tctatgccga cccgtccagc atcgtcggca gcatcggcgt
     3061 tatcggcagc agtttcgacg cgaccggcct gatggaaaaa atcggcgtga aacgcagggt
     3121 taaaattgca ggcagcaaca aaggcatggg cgatccgttt tcgcccgaaa cgcccgaaca
     3181 gtcaaaaatc tgggaagaaa tgctgaccgg catacacggt gagttcatca aagccgtcaa
     3241 aaccggacgc ggcggccgcc tcaaattccg acaatatccc gacgtgttca gcggtcgcgt
     3301 gtacacaggc gcggatgcgc tgaaagtcgg gctggtggac ggactcggca atatttacag
     3361 cgtggcgcga gacgtggtca aagcccccga tgtggtggat tacactccga aggacgattt
     3421 cggcagaatc ttgggcagac gcttcggcgc agaactcaaa gcaagcgtgc gcgaagccct
     3481 gcaggccgtc cggtagcgtt gtttacagta tttctaaggg aataccaaaa taaaaatata
     3541 cacaaaaaca ctcacctccc cagtatgctg ccactaagtc agccgcgtca ctatttaccg
     3601 tgcacttaaa gccgtaaaag gcaggttgtc ccgggaggtt ttgttcatcc tgataccgat
     3661 cggttgttgt tgctcaaagg acagaaggcc gccgataaac gagattacct gtttgtcgct
     3721 attgacgatt tttatactct gccattttgc cggacaaaac cgcagacagt gctgccaagt
     3781 ttctgaccga acatctggct gacccctgct tgtacctgat tgagtacgtt tactctgaca
     3841 atgataggta atataaagag ccgtccaaca tgctttcggt gcagcttgtt ataataatgg
     3901 gattggttgg aggcttgccc gatttgcttg tccgcagacc aacggtaagg cggagcgggt
     3961 tatccgtacc ttgatggaga tgtggcatga ggaacagtcg tttgacagtc cggaacatcg
     4021 gcaaaaggag ttgtgctgct ttattaattt aggggctgcc ctagatcact agggaaattc
     4081 aaattaagtt agaattatcc ctatgagaga aagtcgtcta agacggtata aacaaaataa
     4141 actcattttg ttatcgtggc tatgatgtat tggatgcggg cgaatttagc catttccgta
     4201 tcaatcacag cacacatttt gccgaacgac aaaaccatat taatggaact gagaactttt
     4261 ggaatcaagc aaaacgtcat ttacgcaagt ttaacggcat tcccaaagcg cattttgagc
     4321 tgtatttaaa ggagtgcgaa tggcgtttta acaacagtga aataaaagtt cttgttccat
     4381 tttaaaacaa ttagtaaaat cgagtttatc ctagttgtcc aaggcagcct catctcttta
     4441 tagatagctg cttaccaagt atccttaatt gctaattcaa gccaatcttc aaaactatta
     4501 aaaaattcat ggttttctat attatctaaa ttaatgacaa taaccctagg atttcctgtt
     4561 acatcattac catcaaaata agcttcccat tctccattac tagcgaatgg gataagattt
     4621 aaatcaggca ggatagaatt tctaaatata taagctactt ctgccccttc tgttccataa
     4681 ttctcaaacc accagtgaaa attatgtggc tgaatataag gtattaaaga tgtatcgtta
     4741 gagatacgaa catacttttc aggataacaa aacctttcag gaagcatatt actaggatat
     4801 aaattaaatt cattttgaat aatcatttcg ttaccttagg atcataaata ctaccattag
     4861 gaaactgggt agaccaattt ggtggtgcta gttcattttc aatttgtttt ttaattctaa
     4921 attgaatagc ttctccccat gtttgcggca tctaatgctg cttgctctgc atcaatatta
     4981 ctaaaaattg gattagtctc aaaaatgaaa aattactgtt ttttcatttt ttatagcata
     5041 ttcaagtgca tgaataatat ttttcactaa gggctctgta gaaaaatctg aattttgtaa
     5101 tttttctttt acagccaaaa tactttcctt atctattagc caatccccct cacttggtcc
     5161 aaataaccaa ttatcggtaa tttcagaaac aagcccccat aatccacgat tttgatattc
     5221 aataatttga ttttccgtaa tttctacaac tgtttcagat tgaatgtttt tataagccca
     5281 atcaatcatt tcagtatatt cactaattga aacattgtct tttaaacaga aaattaacag
     5341 gttgtgttca ttcatttgtc taagttcctg atatgagttt gttgttgtaa ataatctttt
     5401 gcttgcttac cttgtaaatt aaaggattaa ccgcaccttg gatgatgccg cgaatatcat
     5461 ccatacggtt accccaccaa tttttattgg tgtatcgtta atcatttatt tcaaaaaagt
     5521 tatttattat catattatca gggagcaggt cgttaaaaaa aaccccctca aattccatta
     5581 aattagtatt tatccagcac ataaacgaac caaagcgttc tttccctgag tgtaaatatt
     5641 taaaattaaa ctcaaaataa gtttttttgc ttagaaaatt taatggtttt tctatagtga
     5701 aaaaaaagcc cttactcgtt ttcgttcgag atactatttt aaataaattt ttattttcaa
     5761 taaaataaga atattcattt actaagttgt tatgataaaa aagtaatttt attaaaatgt
     5821 tttcataatt ttctaggttc atttctagct caatttaaaa tgatttcatt gttgccgtac
     5881 caatgcgaaa tttaccatca acaaaatttc ccttcactga tacaggtaca ccatcaactt
     5941 gggtagtttt attttcaggc tgcctgaaag aaagataggc aacttattac tcatcttacg
     6001 ctagccctca ataattgaat acgttgcact agaaccaatt atttctatga atgtttgctc
     6061 aaacataaaa atatagtatt taaagaattc tctattcaaa ggaaaataac tttctttcag
     6121 tatccattcc gcataacact catcaagaag ttcaaaaaaa gattgaccac cattttcatg
     6181 aagcaaagct ctatctgcat cttccgaaat taaatccata aaagtatcta taaaatacga
     6241 ttcatcaaca accctaaaat gaataacatc ttcaataaaa atttttatgg ttttatccgt
     6301 atttctataa ccaactgcta taacttctac tttatcagag tttattgctg cattatcaaa
     6361 atagaaatag ccagttggga aatctaagct ttttaatttc ataatttatc gtgtatttcc
     6421 atatctgatt tttatttttc ggttattact aattgttatt tcaagtgttg gttctccacc
     6481 actactatca ttacgagcat taaccgcacg cccatcaggt aaattgcccg ttatcgttcc
     6541 attaggacgt gtttgtacat ttcgaacatt cagactatta aaatcattta gtgcgtcatt
     6601 gtaatccccc actttatcat actgaacagc gggacctttt gttttactcc tttgagtagt
     6661 attgctcaaa atgtctttga tttgtaaatc actgcgcgcc ttaccggctt ctatgcgagc
     6721 tgccacacta ctcgggctat aggggtcgct atcaatcgca ggattaccac cgctggtttt
     6781 ttgtccgcta gcctgtttcc caacctgctg tactgtatcg ccaatttcat ctaccgtacc
     6841 taacgcctta gtctgcatat ggcgaacagc aggtttttta tagccggtat ttttaaccca
     6901 atcccatttg gtcgggttaa gttctacttt tttacctctc caaaccgtac ctgcggcctc
     6961 tgctacggca agggcagttt gggctgttgc tgttatattc ggatgggcat cagcccattg
     7021 tctggcggaa ttgatgccgt cttttaccgc aaaagcactg tcctgtaata tgctcgcggc
     7081 agcaagttgt gcttccgggc ttaattttcc tgaattatta ataccttgta gagtctgctg
     7141 cgcagagatg tagcaactgt atttttaccc cgtcgggtaa aaatacagtt gctacggctg
     7201 ctgggaaagc agttactatt ttcccagtgg ctcaaaatta attgtttcag cagatatttt
     7261 tataataatc cctacatcaa taataatttc actataaaat tggtatttat atagctctat
     7321 ttcgggtata aacaagggaa acaagctatc ttccttatcc tcataatcta cgaaattatt
     7381 tgtatctaat ttaaaattca aaatattact gcatttgata tatccgaaat aattaccttc
     7441 aattgtatct gtaaaagata atgtaagggt atttccatta tcggaaaaga ttatttcata
     7501 aaggcttaca ttgtgaaaaa tatttttttt catttgacaa aatatccaga atgatgatga
     7561 ttatttggca atggattgcc ttcataatct tgtcgttgcg gatattttcc ctgtacacca
     7621 tcggttcgcg gcactctttg ggttcttata acctctccat ttggacgaat atctattttc
     7681 acgccatcag gacctttcca tgtttcatat tgagccgcat ctgaaccagt acgtctaaaa
     7741 ccttgttctt gcaatgcttg acgtaattca tctcgcgtag ttggtctgat agatttaaca
     7801 actggcttgt ttcctcctgc catctcaccg tccaacgtct gcatagggcg ggcagcaggt
     7861 gttttatagc cgttattttt aacccaatcc tatttggtcg ggttaagttc tactttttta
     7921 cctctccaaa ccgtacctgc ggcctctgct acggcaaggg cagtttgggc tgttgcagtt
     7981 atattcggat gggcatcagc ccattgtctg gcggaattga taccgtcttt taccgcaaaa
     8041 gtactgtcct ataatgcgct tgcggcagca agttgtgctt tcggacttaa atttcctaaa
     8101 tcattaatac cttgtaaagt ttgctgcgca gagatgtagg tcggatattt gtatccgaca
     8161 aaaatatttg acgcatctat cgtttccgaa atgccgctgt tggaaaatgt cggattcgag
     8221 aatccgacct acggctattt cgtttaagtc ataaatagcc attcatgttc cttctgaatt
     8281 tgcttttgct atccttagaa ccaatcatat aaacattatc atcattaatt aatgttctaa
     8341 gtttatgaag cggtcaaatc tgtcaaacta ccagagatga agcatatcct cctggaagta
     8401 attctgttcg tgctgtttcc atatcttctt tgccatcatc agttacttct ctgtctaaat
     8461 aaaatcttac gataagtttt tgtctaattt catcaaattc tgccgtaatt aatcttatat
     8521 tgatatgaat acacggttca tacctgctat tagccagtca ggtgtagttg gatggggatt
     8581 catttttaat aatatcttca agcaagtgaa atgtaagaaa agcgtagata cttgaaggcc
     8641 ggctatctga ttggcaaaaa gaaatttcgg agataaagaa tttccgttta tatttttcat
     8701 aaattaaatc aacaccttgt tttacataaa aactaacttc ctcaggaagg atatttataa
     8761 cattctctcc ttttactttt atagttaatg gtttgatttt tatgttttca tgaatatctg
     8821 ataggattat ttctaaaaaa ttatttttat ttccagtcat tctggctatt ttatgtatat
     8881 ttccattgtg ttcgattatc attttatttt ttccaaggat ttccttctat attgatagtt
     8941 tatctaaaga tttcatttaa aatatcctga ctaatttctt gtttatataa ttctacaatt
     9001 ttctgtttct gtgtatttgt ataaaatttt tcgttggtat ctgcttgata ttttaattgt
     9061 ctcaatattt ccatgatttc atattctgat ttttcatcat attcttcaat catgtaatca
     9121 acaaattgtt ttagaatatc aaaggcttcg taactttcca aaattttaat atgtttatcg
     9181 gcaaaattgg acatatacca ttcgtcagtg attggattag tatttaaatc atctaaaaaa
     9241 acatttaaat catctaaaaa aacatttaca tcatctaaaa acatgactaa tccttaaact
     9301 taaaatctgt tttctcaccg gttcttgagt tttcaatgta atgtatttca attttgttta
     9361 cgttttgaac acgcttaacc caaccgtctt ttgctaacca accattttta gtatcggaca
     9421 taggaggtat tctcgcagga gttttgccct gtgggtttgc cttaacttct tccattgcta
     9481 attgttcctt taaattatta ggtattgtac gaccggttga tttttcaaca gattgtgtcc
     9541 ctatttttaa tgatttgttt cccccagcca tttccccgtc tacagtctgc atagggcggg
     9601 cagcaggttt tttatagccg gtatttttaa cccaatccca tttagtcggg ttaagttcta
     9661 cttttttacc gcgccaaacc gtacctgcgg cctctgctac ggaaagggca gtttgggctg
     9721 ttgcagttat attcggatgg gcatcagccc attgtctggc ggaattaatg ccgtttttta
     9781 ccgcaaaagc actgtcctgt aataggctcg cggcagcaag ttgtgcttcc ggacttaaat
     9841 ttcctaaatc attaatacct tgtagagtct gctgagcggc tgtatctgag ccggtcagtg
     9901 taggttggat taaaacccag cattttcaac atcttgatgt tttactataa atctattctt
     9961 gtgcttcctt tgttagataa atttcaacac aaaagatacg tatgcattct ctaattttat
    10021 ttgagataaa taatgcattg ctttgtcgat actgttaaat aaattacttc cctcgtaata
    10081 ccaattatca tatgtttgat aaaaataatt atctttcttg atataaatat ctccccccaa
    10141 tacaagtatt ctctcattga taaataaatt taatatatct attgcaatag ggacgggtaa
    10201 tgcggcttct ctaattccaa ttcctatcaa agattcccct tgtctcgaat aaatttcaaa
    10261 aatttcattt aacatgttaa tctctatcta ctatttttca atcggtataa accctattca
    10321 cagtttcatc ttgtatttct ttatgaatac ttaatatatt aagatagctt aggaatttta
    10381 ttttctcaga ttgaggcaac tcatcaaaac caagccaaca ataaaaattg ttatatgatt
    10441 ttaaaaaatc aacaattttt atttttccta aaaccaaatc ttcgactaac tgggttgcat
    10501 aaataatatc atttatgatt ttaacaggta aattatttga taggtaacca tgggattccg
    10561 tcatcattga tttctctagg tgcttattta tctgattggt aagccttttg cgtaagcttc
    10621 tttcgcctcc gcaagtgtca tttcattagg gccaccatct atattagttt ctcctgtatt
    10681 ttgccactga caaacgtcac agatatcata gtccataact tcagttccgc aaacaggaca
    10741 atgtagccat atttctccgt caatttccca tgtctttttt gaattctcca tcataatact
    10801 tctttcctaa gtttggttta aacattgttt tgattttgaa tgtttttgga ttggccaaaa
    10861 catagatatt gttattgata tcatagcgca ctcgtctatg ttccgtctga taacctaata
    10921 tcttgtccga tgtctgtttt gataacaaat cagacgccat tttttggtag tcttcaatgg
    10981 ttatatcgcc aaattctttt ccatgagctt tgaaatgtcc atttaaagat ttctcagtag
    11041 gaaacttgga ctttgtccat ctgatgcggt cttgaattgt tcctgtcgga gaaatagatg
    11101 tatcaggttt aacatatgag gcaggtaagc cgatattgtt atcagagtgt gtagggtgtt
    11161 gctgcgtact tggttttggt ggcttattcc cacctgccat ctccccatct aaagtctgca
    11221 tagggcgggc agcaggtgtt ttatagccgg tatttttaac ccaatcccat ttggtcgggt
    11281 taaggtttac ttttttaccg ccccaaaccg tagttgcggc ttctgctacg gaaagggcag
    11341 tttgggctgt tgcagttata ttcggatggg catcagccca ttgtctggcg gaattaatgc
    11401 cgtcttttac cgcaaaagca ctgtcctgta ataggctcgc ggcagcaagt tgtgcttccg
    11461 gacttaaatt tcctaattca ttaatatctt gtaaagtttg ctgcgcagag atgtaggtcg
    11521 gatatttgta tccgacaaaa atatttgacg cgtctatcgt ttccgaaatg ccgctgttga
    11581 aaaacgtcgg attcgagaat ccgacctacg gctgctacca accatcattt tcagcttctt
    11641 ttagccaagt atcaaaattt tcaaaagtct cgtaatattt tgtattatct aaattaacta
    11701 ctataatttt aggattacct gatttatctg aaatatcaaa acaggcagcc cactcttggt
    11761 ttctagcaaa aggtaatagg tttggaatgc cagttatttc aaaataaatg tcaattactt
    11821 ctgatatatt gctttctgca ttttcaaacc accaaggaaa agaatattgt tcatcgtagt
    11881 taatggcatg agtggattga gctaatttta aataactttg cggatattta aatcctttag
    11941 gaagcattgc ttcaggatac ggaacaaaac tattttgttt tatcatttcc taagtacctt
    12001 tacatcataa atacttccgt taggaaatct tttgctccac ccttctggaa ctcctttttg
    12061 tgtttcttgc tttctaattc gtagctggat agcttctccc caagtttgag gctcaatacc
    12121 atcatgctta aacctgattc tctcttttga tgataatcgg tcattaaaat tatgaacatt
    12181 cgcttccact tttacccctg cttcacgtgc tgccataata cgcgtgttat ccatactagt
    12241 aggtgctcca tcaggcatat taacaacatc aaccctgtcg ccaacccatg atttttttct
    12301 ttcatttcgg ctacaataga atcatagttg taatttgcac taggctctga caaaaatttg
    12361 ggttgttaag ttgtcatttt tttataaatt ccagcaaact gttgttgtaa attatctact
    12421 tcatgttttc taacaccaga tacattttca aattctcttg aatctacaca tacgcccgca
    12481 tatatctcat tcaagatagc tcgtaaaaca aaaacttcat cttgagttag tgaaatagtt
    12541 gagttttgat tatttatgga tagtatattc atggcttttt cctataaatc atcataatat
    12601 tttttacctg atgttggtct aaatgctgta cctccatcgt cagaattttt atctcggata
    12661 actattgtcc ctgttttatc atcccaatac gcagttctac cgcgaggtaa ctctttcata
    12721 tttgttggat ggctaacaat attttcaata tgccgagcaa aatctgctgg tgaattgata
    12781 tttaaatccg taaattcttg ttgtcttatg acatgcttgt tataagcatg cccactacta
    12841 atttgttctc caattagttg cgcttgtaaa gacggttgtg tggaagcatc tgctttgctg
    12901 ttggacgtta tagatttagg cggt
//
LOCUS       DAXHPG010000042        12823 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXHPG010000042 DAXHPG010000000
VERSION     DAXHPG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.042"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            389..1105
                     /locus_tag="ACFM6F_000901"
     CDS             389..1105
                     /locus_tag="ACFM6F_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC6164344.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            1208..1633
                     /locus_tag="ACFM6F_000902"
     CDS             1208..1633
                     /locus_tag="ACFM6F_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164345.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYDPTD"
     gene            1877..2077
                     /locus_tag="ACFM6F_000903"
     CDS             1877..2077
                     /locus_tag="ACFM6F_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164346.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            2145..2504
                     /locus_tag="ACFM6F_000904"
     CDS             2145..2504
                     /locus_tag="ACFM6F_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164347.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVSNTSSTLKMVRSN"
     gene            2529..3383
                     /locus_tag="ACFM6F_000905"
     CDS             2529..3383
                     /locus_tag="ACFM6F_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFC6164348.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTPLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGVIA"
     gene            3453..3668
                     /locus_tag="ACFM6F_000906"
     CDS             3453..3668
                     /locus_tag="ACFM6F_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164349.1"
                     /translation="MTITEEYSIDIRVTSEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRKALICLTKDNLKNAA"
     gene            3703..3963
                     /locus_tag="ACFM6F_000907"
     CDS             3703..3963
                     /locus_tag="ACFM6F_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO1622 family putative holin"
                     /protein_id="HFC6164350.1"
                     /translation="MKYLIRTALLAVAAAGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            3960..4253
                     /locus_tag="ACFM6F_000908"
     CDS             3960..4253
                     /locus_tag="ACFM6F_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164351.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLVALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            4256..4447
                     /locus_tag="ACFM6F_000909"
     CDS             4256..4447
                     /locus_tag="ACFM6F_000909"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HFC6164352.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            4447..4587
                     /locus_tag="ACFM6F_000910"
     CDS             4447..4587
                     /locus_tag="ACFM6F_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HFC6164353.1"
                     /translation="MDTLLKIIIALSFSGAEALAVWLFITAADAVLRRFPKPSHYRRV
                     KK"
     gene            4584..4793
                     /locus_tag="ACFM6F_000911"
     CDS             4584..4793
                     /locus_tag="ACFM6F_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HFC6164354.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDGRIP
                     CRLKTPLTSPVWDTREVLEALGLQQ"
     gene            complement(4851..5768)
                     /locus_tag="ACFM6F_000912"
     CDS             complement(4851..5768)
                     /locus_tag="ACFM6F_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFC6164355.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMSRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVSYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPILPEIRNLSSV"
     gene            complement(6637..7116)
                     /locus_tag="ACFM6F_000913"
     CDS             complement(6637..7116)
                     /locus_tag="ACFM6F_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFC6164356.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(7552..7932)
                     /locus_tag="ACFM6F_000914"
     CDS             complement(7552..7932)
                     /locus_tag="ACFM6F_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6164357.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADYHHKMSGNPLPDKPHIQCWAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAIKTAVAKAFKLY"
     gene            complement(8089..9285)
                     /locus_tag="ACFM6F_000915"
     CDS             complement(8089..9285)
                     /locus_tag="ACFM6F_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC6164358.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWVYPTGGRSWK
                     LSFVQDGRQQTISLGRYPDFSLADAREWREEVRRKRAHGENVVNKKVRADFAFEKVTR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMEINPVAQIGSGVFERAKTGNMAALSPSELPRLIDFLE
                     QRNEFAVYAGRVRIHPVTRLCIYWLLLTMTRIQEAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMQWVLDQARALNVNGRFVFESVNFQGHINKESPRVAMQRAGLDTTAHG
                     LRSLARTYLREVLKVDNDVAEKLLAHSLGTRTQTAYNRSELWEERKDALERWGNDVLR
                     LAKNGK"
     gene            complement(9476..9565)
                     /locus_tag="ACFM6F_000916"
     tRNA            complement(9476..9565)
                     /locus_tag="ACFM6F_000916"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9529..9531),aa:Ser,seq:cga)
     gene            complement(9669..9759)
                     /locus_tag="ACFM6F_000917"
     tRNA            complement(9669..9759)
                     /locus_tag="ACFM6F_000917"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9723..9725),aa:Ser,seq:tga)
     gene            complement(9842..10621)
                     /gene="yaaA"
                     /locus_tag="ACFM6F_000918"
     CDS             complement(9842..10621)
                     /gene="yaaA"
                     /locus_tag="ACFM6F_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC6164359.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AVEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(10785..11972)
                     /gene="dapC"
                     /locus_tag="ACFM6F_000919"
     CDS             complement(10785..11972)
                     /gene="dapC"
                     /locus_tag="ACFM6F_000919"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC6164360.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLDPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            complement(12049..12501)
                     /gene="dut"
                     /locus_tag="ACFM6F_000920"
     CDS             complement(12049..12501)
                     /gene="dut"
                     /locus_tag="ACFM6F_000920"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC6164361.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPSYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFAVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
BASE COUNT         3510 a         3295 c         2933 g         3085 t
ORIGIN      
        1 atattaaaaa ttatcgtcat caatgcggct tccccagccc gcggcgagct ttccacgaca
       61 ggcttttttt atttgggttg gtcgtctggt ttctttgagt tcaaaaacag gtcgggatac
      121 ttaagtttta ttcgtgcagg tatcccgcgc ttcgtccaat tgaaaacgca ttgagggcta
      181 ttccctgtta ttcgaccaac ttccgcgtaa ctgccgattg attgcaacag gcgtttgtct
      241 tcattgactc ttttatccat aaataaacca aatgtttaaa tctaatgcta atattaaaca
      301 ctatgtttag ataaaaatca agtcttgtgt aacaacattt tgtttaaata tgggagaata
      361 attaagccaa ccgcgaataa gattaaaaat gacaatgcac gagacaactg acagactttt
      421 tgagatagcc aaagagcagg gagttttaaa gccggctgac atagcagagc gtctgaatat
      481 cagccaacag gctttgaaaa actgggaaag tcgtggcata gcggcaaagg cgctgcctga
      541 agtagcaaaa gcattcggtg tatctgaaac atggctgaga acaggtgaag gcagccgaac
      601 cgcgcccgtc cttattgacc ccgacctacc ccacgaagtc aaagacatcc accgcccgat
      661 gacgtggagc agcaacgacc cgctgcccga cgatgattat gttttcgtcc cctacctcaa
      721 agagagctgc ttcaaaggcg gagtaggcac gtatgaaatc cccgattaca acggctaccg
      781 cctgccgttc ggcaaatcca cgcttaaacg caaaggcatc aatcccgaca acgtgttttg
      841 ctgcaccctg accggcgaca gtatggagga aaaaatcgca gaagacgcgg caattgccgt
      901 agatacgggc gaaaccgcca tacgcgacgg caaaatctac gccttcgccc aggacggtat
      961 gttccgcgtg aagtacctga tacggcagcc tggcaacagc gttctgatac gcagccacaa
     1021 cagcggtttc tatggcgacg aaaacgcccc cttggacagc ctgaccgtta tcggcagggt
     1081 attttggtgg agcgtgctgg attgaaaaat gttgctatta cgacgagata atattgatag
     1141 ggctttcaag attgtcaaaa ataggcgttt tgattcccct tggtggcctg gtgagtacga
     1201 tgccggtatg aatttccttg gcgtacaagg agagttgaag gttcacgagc tgcatcacag
     1261 aacagcaacc ctgtgctttg agtggcttgg cgaagtatct gcgccgcgca gaaaagagaa
     1321 ctacaaagac ctcaagccaa atgtattgta tgactttgat ggttccggta agcattttgc
     1381 caaccctgac gcaaggtatc tattgcccgt cggttcaagc ggcttaatct taaaacatat
     1441 ccaaatcgat gacgaagaca ctctgttgcg gctatggtgc gcacgaaata tcccaatgcc
     1501 acaccggcta tctaaaatcc cgatgcttcg gcagtattat ctaagcaagg catggcacga
     1561 aatttatgct atcaaccaac acctaaggaa aacgaagctg atagttgatg tggcatacga
     1621 ccccacagat taaacaaccc gcccgcatta tgcgggcttt tttcatgccc cgccgaacct
     1681 gaaaacaaca caaaaccgac aaagccgcct taataggcgg tttttttgcg cctgcgaaat
     1741 ctcaaaaata aattctttta ataatcaaaa tgtttataaa ccttaaacaa aaatcttaaa
     1801 cagattgttg catattatta aacgctatgt ttaaatacac ccatcgaaac aaacaacagc
     1861 cacatggagt accgacatgg aaaccatcac catccctaaa gaaaatttcg acgctttgct
     1921 aaatctcgca agaaccgccg tcgccctgaa cgaaaatttt attccaaaaa taaattacgg
     1981 agcatccacc ataccagcag ccggctttgc cgcacttaac gagtttggaa tagcccgctc
     2041 caccgtggaa tctttggaaa ttctcaaaat taaataacct cctcggtatt gtcatcccgt
     2101 gataccggca acctaactaa cctctcttta aaggaaaatc aaaaatggaa acccaagaaa
     2161 agaaatttgt tcctgcctcc aagcaaatac cgaccgtaaa tagcggctgg ttagaccgtg
     2221 aattaggcga agccatgagt gaagcggtgc gtgcctgcct tgctcacggt aaacagtcag
     2281 aaattactgt caagctcaaa atccaacccc aaaacatcca aagcggaacg gtaaaaatca
     2341 gccacgatgt agcaaccaaa ctgcccaaag aaaaacgcga aggcggcatc gtctttgcta
     2401 cacctgacgg caacatccaa gccgacgatc cggcacaagg aaaattgaaa ctcaaacaag
     2461 tttccaatac gtcaagcacg ttgaaaatgg tcagatccaa ctaactcaaa cacaaaagga
     2521 aatctaaaat ggaaacccaa gaaaacatga ttaaaaccgc cttacaagca gctcaaaaac
     2581 ctttttttga gtttgcaccg aacaatactc cgcttgtatt cacaccagac caagacggtg
     2641 gctggagata caaatcacac cccgaattga tgcaaaaccc gtaccgcaag tgcggcaaat
     2701 tcctcatgca cgataccgcc agtcttatta agtttgtaca aaaacacaaa caggacggca
     2761 cacaaatcta cattgatgcc gatttcaaat cagggcgtat tgatgtcacc gccgtcatca
     2821 atggccatac gcaacaagct ccgggctggc tcgattttta tgccgtatac accccgcaac
     2881 acactacatc ggcatccaac tggctgaaca acaatgcgca ccgcatgaat cagatggagt
     2941 tttcccattt cctgaccaac aatgcccgca acatcgtatc caaaaatccc ggtaacgaaa
     3001 attccgttta cccgaccgcc gccgaagtat tggatttcgc gctcaatctc gagtacaccg
     3061 aaaaaaccac cttcaaacaa ggctatcgtg aacaggacgg cagaatcaac tttaccttcc
     3121 aatccgaaga ctcagggcaa acagagaaaa acctcaaaat gtttgaacgt ttcggcatcg
     3181 agttcacccc gcatcaaggc ggtgcatcct actttgttga agccctgttg aaattccgca
     3241 tcgacaaaaa cagcggcgca ctcgtcctgt ggtacgaatt gcagcaaatc gatgctgtaa
     3301 tcgaacaagc ggcaaaagac atttccgaag ccgtacaaaa agccttccct gaaatcgaca
     3361 tctatttcgg cgtaatcgcc taaaaaaaag tcgtctgaaa tcgtgaacct tcagacgacc
     3421 tccacaaaaa cccaaatccg aaagacccca aaatgaccat cacagaagaa tactccatcg
     3481 acatccgagt aacctcagaa caggggaaaa acgactacgg ctaccccacc gaacgctacg
     3541 gatgcgacat catcaacaca gacggggagc tacttgtcgg catagagcca gagtacaaaa
     3601 ccccctttgc cgccgtcaga aaggcgctca tctgtctgac aaaagacaac ctcaaaaacg
     3661 ctgcctgata ttaagccgtt accccaataa tgaggaatca aaatgaaata cttgatccgc
     3721 accgccttac tcgcagtcgc agccgccggc atctacgcct gccaaccgca atccgaagcc
     3781 gcagtgcaag tcaaggctga aaacagcctg accgctatgc gcttagccgt cgccgacaaa
     3841 caggcagaga ttgacgggtt gaacgcccaa atcgacgccg aaatcagaca acgcgaagcc
     3901 gaagaattga aagactaccg atggatacac ggcgacgcgg aagtgccgga gctggaaaaa
     3961 tgaactttca ggaagaaaac gagaacatca agaatgacat agagattaat ctcttgcatt
     4021 ccgctgaagc cgacagcgtg gaggcaatga tggacttggc ggtttacggg ctggcggcga
     4081 tggttgccaa acacaacaaa acgcgcggcg agcagcttgt gttacaggcg attgccggtc
     4141 atatgcggaa gcttttggtc gcgcttcccg aaaccgagtc attggtgcag accgggaagg
     4201 tttaccgccg acttgaagat ttgattttta aaacgtattt gccggagcag taaagatgaa
     4261 cacgaatcaa gatacgattc catttggcgg caaccttgtg atttgctgtt cgacgggaaa
     4321 cggcggggac gggcggtttt cttgtctgat taccgatcag ataccggatt taaatcttgt
     4381 cagaagcggg caggcgttcc cgatgttcat ttatgagtac gaaactagag aaaaaaggaa
     4441 agcctgatgg acaccctatt aaaaatcatc atcgcgctgt cattttccgg cgctgaggca
     4501 ttggcggtat ggcttttcat cacggccgcc gatgccgtat tacgccgctt cccgaaaccg
     4561 agtcattaca ggagagtaaa aaaatgaaca tcaacgaact gggcgcaagg atagaccgcc
     4621 cgaccatccg cgaactgatt gcctacgcaa cctgccgcaa ccgccccatt tcaaactcga
     4681 cactgctgcg tatggaaaaa gacggacgga taccgtgccg tctgaaaacc ccactcacat
     4741 cccccgtatg ggacacccgc gaagttttgg aagccctggg gttacagcaa taaaaaaacg
     4801 cccgattagg gcgttttcac attcagacgg cttttgtatt ccctactgca tcaaaccgac
     4861 gacagattgc ggatttcggg cagtatcggg cggatttttg ccgcgtgttc cgcgtcggcg
     4921 tgtgcgttta aggcttcgag ggcgtttgca gcggctttga ggcggctgcg cgtttccgcc
     4981 cagaccgtcc acatcgtaac cgcctgtttg cagccgagct gcttcagcgg cagggaaacg
     5041 tctctgccca tttggatggc ccacgcgccg tagctgacgg caacggcgag gtcgtacagg
     5101 gcgttgccgc ttatgggcag ggcaggttgc ggcgcgggta acggttcgcg gtcgaggact
     5161 tcgccgtaca gcacgccgtt ttcaactgcc acgcggctca tcacgcttaa aacttcgggg
     5221 aactgttcgg caggtacttc tttgtagctg catccgaact tgcttttgac ggcagaccaa
     5281 agggtaatgg cgatacgcgc ctgcgcttct ttgggtgcgg atttggtcag ggcgttgtgc
     5341 agttttttga cggcttcgat ttgttcgccg gtcaagccgt ccggaagggc gcgggtgggt
     5401 ttgggggtaa ctgtttttat gccgtctgaa attttgccgt taagtaaaat ttcaatctgc
     5461 tcatcgcacc aaaccgcaaa tttcggatta agccaacggg caaagtgaat agcgagtttg
     5521 ggatgcagcc atgtgccttg ctcactgcca ccacgcttca cgataactat ttgatttgct
     5581 tccgttagga tttttctcct aacgcttaaa ttctcagcaa gtgcagagat atattgttga
     5641 gtttgttcac tttttaggta gtctttaggt aacttgccaa agtgagatgc aatggcggtt
     5701 gcatttaaaa aaccgtcttg acggaaagat acaggggtgt taccaaaatt gagaacagat
     5761 acgttcatga tagtttccta gtttagtttc gaaagaccca aatgggtggt cgggaggttc
     5821 gaaaacctac tagaaaaggc cggcgttatt ccccttgcgg gtattgtatt ccgcaccctc
     5881 ccgaccatag gaaactagtt cggatatatc gaaacaaaca accaaacaag aaaacttatg
     5941 gacgtaaaaa attcacgctg acggggtgaa tgccgttcta gtagaggttt tcgacgcctc
     6001 gtgtttcgta atatatccaa tctttctatt ttctgcaagc ataaaaaagc cgccatgtgg
     6061 cggatagagt cgcggtcttc aacagcatgt aggatttagg aatgcccaaa ttgggcgtag
     6121 gggtgttgaa aacacgcaaa cagacgcgcc gccagcctta cgggtaggcg cacccccaca
     6181 taggagtaaa tcgatttgat acgtatagac gtaaaaaatt ccactctatc gtgttggata
     6241 tacgctgttt gtcaggtgtt ttcaagcacc gtgggaaata ttatatagag ttatggaaat
     6301 gtgtcaagag aattagccca aatgggcgga cgcgcggtta agaacccaca taggagggcg
     6361 gacttattcc cctttcgggt cttgtattcg tcgcccacgc ggtcatagaa acttcctgct
     6421 atcgaaacaa acaacaagga aagaaaacta tagacatgaa aaaatcacat tgacggagtg
     6481 attgccgctg tagtgtggtt tctgacacca cgaacaggaa tataaaacaa aaccccctgc
     6541 acatgcaagg ggtttcccaa aaaaccgtag gcggcaaact gaaaggccgt ctgaatttca
     6601 gacggcctat gttgcggcgg atgttaagct attaagctat ttggttttca gaggcttttt
     6661 cttccatttt cgggctacgg cttcaaattc ttgaaaggcc gtcggtgcgc tcctgatacg
     6721 gcgtatttca aaaatgaagc tgcttaggtt gtcccagtca cgtaacagca tggagtattc
     6781 gaaatcttta tacattttct cgtgcagtgc tccgcccaat acgcccgcac agacaaattc
     6841 gcgctggttt aaaactgtca gtatggcttc acggtctttc ttcttgtccg gggtatccga
     6901 tgtataggtg gcgagtatgc atccgtctgt ttttgccagc ccgtttacta tggttatggc
     6961 ttcttgaagg gcggcattgt tacgttcggc catgatcatg tcgatggtgg cgcgcttttt
     7021 ggcgttttct gtattcctca tgatgccgta agcggcaaca aaaacgccga taacagtcaa
     7081 tatcggcgtt gctatctgta ttaggttgtc agtcattgcc gtcccagccg tctgaaaggc
     7141 ggaaatcacg gctttccgaa taaatatgtc ccattgtcat ccccttaagc tgatgttgaa
     7201 aataaatcct tgcgtcggaa tatacggcat tgcttgacta ttttcaagat tctgtacctc
     7261 gtgaaccgga ctatacaaaa aaaccgcctt cgtgtaaagc ggttcttaaa tcgcccgtta
     7321 aggcggacac aacgatagcc aaactggaac aggaggcggc aaaggcgggt ttgtccaaaa
     7381 cagccttgat tgaaaggctg attggggatt tgtaggcgtt tgcagatacg acaaggggcg
     7441 cattccgcgc cccatcacac gcacggacgg cttgtcatcg cctgtcgccc gtgcgcggct
     7501 gcccatattt cagaacagtc ttgcaagccc ctttaaggga acggttttat tctagtacag
     7561 tttgaatgcc ttggcaacgg ctgttttaat cgcctgaaaa tcctcttcac tgattattgg
     7621 aatgcagcgg tcgcgccctt tgggtttgta tcggtctaat cgtgccaatc cgactgttgc
     7681 cgtcatgtcg cattttgccc aacattggat gtgcggcttg tccggtaagg ggtttccact
     7741 cattttgtgg tggtagtccg ccaaagggac aggttctgtg ctgcttaagg gtacgaccgt
     7801 taccagtttg ccgttgtgcc tgtttcgcgc tatgacgacg acagggcgtt tcttgaccat
     7861 ttccggttct tcataaccgc gaaagtcgca catgataacc gaacgttccc ttggttggaa
     7921 ttttaaaggc attagccgct cctgacgatg acgggctgct attatagcag ttcttacaca
     7981 aaaaaccgcc tttgtgtaaa gcggttgcaa aaaagccttc caataaaaat gcggaaacgg
     8041 ttttttattt gcgtttccgc ataatacaaa cacacacaca aaaaaaaatc attttccgtt
     8101 tttggcaagc ctcaaaacat cattccccca ccgttccagc gcatccttac gctcctccca
     8161 aagctccgag cggttgtaag ccgtttgcgt cctcgtcccc aacgaatggg caagcagctt
     8221 ttccgccaca tcattatcca ccttcagaac ctcgcgcaaa taagtacgcg caagcgagcg
     8281 caaaccgtgc gccgtcgtat ccagccccgc ccgctgcatc gccacgcgcg gactttcctt
     8341 attgatatgc ccttgaaaat tcacactttc aaacacaaac cgcccgttca cattcaacgc
     8401 ccgcgcctga tccaacaccc actgcatcgc ccgcgacagc ggcacatcat gcccccgccg
     8461 ctcctttttc cgttcggcgg ggatacgcca aacctccccg tccaactccg accactccat
     8521 caacgccgcc tcctgaatcc gcgtcattgt caacagcagc caatagatac aaagccgcgt
     8581 tacaggatgg atacgcaccc tgcccgcata aaccgcaaat tcattgcgct gctccaaaaa
     8641 atcaatcagg cgcggcaatt cagacggact caacgctgcc atattccccg ttttcgcccg
     8701 ttcaaacaca cccgaaccga tttgcgcgac agggttgatt tccatcattc ccgaaccgac
     8761 cgcaaacgca aacaccatct tcagactgtt tttcgttttg cgcaacgtat caacgatacc
     8821 gcgcgcctcc atcacacgga ggcagccgac cacatccgcc gtcctgacct ggcgaatatc
     8881 aagattgccg atagccggaa aaacccaccg ttcaaaattc cgcataacct gtccggcata
     8941 cttttcagac cgccccttcg accaacgcac aaaccaatca cgcgtcacct tctcaaaagc
     9001 aaaatccgcc cgcaccttct tattgacgac attttccccg tgcgcccgtt ttcggcgcac
     9061 ctcctcccgc cattcccgcg catcggccag cgaaaaatca ggataccgcc ccagcgaaat
     9121 tgtctgctgc cttccatcct gcacaaacga cagcttccaa ctccgcccgc ccgtcgggta
     9181 gacccacaaa gccagcccgc ccccgtctga caacttatac agcttatcgc gcggcttcgc
     9241 atttttaacc tgatttgccg acagcggcgt aatgattttc gccattatgg taatttccca
     9301 cttatccaaa aattaccata tacattacca taaaaaaatg caataagaag ataccgcaag
     9361 attgcaaatg atttactttg acgcgttttg atagggcaaa ccgatgattt ttatatggta
     9421 attgacacaa cttgaacaaa aaagaaccgc cctgaatcag ggcggttttg ttttctggcg
     9481 gaaacggtgg gattcgaacc cacggaggat ttgcaccctc agcggatttc gagtccgctg
     9541 cattcagcct ctctgccacg tttccgaaag atgtttcaac ctaaaaggaa acacgatgaa
     9601 gtaaaaccaa ataaaaatgc cgatgcttta tgctacttca tttttatttg gtttttgacc
     9661 tcaaggattg gcggaagcgg tgagattcga actcacggag ggctatcaac cctcgacggt
     9721 tttcaagacc gttgcattaa accgctctgc cacgcttccg tcttcttgaa gagtaggaat
     9781 aataatgaag tttatacatt ttgccaagca cttttttcgg aaaatactta atttattgtt
     9841 ttcattttat ttgttccgaa cgcataaaaa cccattcgct ttcatttgaa gccgcgtcat
     9901 tgaatgcgta gccttcgtaa ttaaaatttt tcagcatttc agggtcggta atatgatgtt
     9961 ctaccgcata gcgcaccatt aatccgcgcg cgcgcttggc atagaaactg atgattttat
    10021 atttaccgtt tttttcgtct ttaaatatcg gggtaatcag ccgcgcccga agttttttcg
    10081 tgttgacaga cttgaaatat tcctgtgagg caaggttgac aagcgtattg ctgcctgctt
    10141 gggcaagcgt gtcatttaaa aggttggtaa tgatgtcgcc ccaaaactca tacaaattct
    10201 tgccgcgcaa attggcaaat gccgtcccca tttccaaacg atagggctgt atcaggtcta
    10261 acgggcgaag aagaccgtac agaccggaca gcaggcggac atggttttgc agatagcgta
    10321 tctgtccaat atccaatgtg tttgcatcca taccttcgta aacatcgccg ttgaacataa
    10381 agaccgcctg tttggcgttt tccggcgtaa acggcgtgtg ccattctgca ttgcgctgcg
    10441 cgtttaagag ggcaattttg tcggaaacgt gcatcagttc ggcaatctgt tgcggcgcaa
    10501 gctcgcgcaa ctgctgcatt agaatgtcgg actctgccag caggtcgggt tgggtaaact
    10561 cgctgacagg ggcagggtct ttttcgttaa ggtttttcgc aggggaaagg acaaaaaaca
    10621 taatctgctc ggtatagtgt caaacacggg gattttaagt ttaaacgtgt ttgcgggcaa
    10681 gccgattaga ttaaaagtga agtaaatacc gttacagcaa attcaaccgt aagatattga
    10741 aaaatgccgt ctgaacaaaa gttcagacgg cattttttta ttctttaccg atatagggaa
    10801 acaatggttt ccgcagcttt gacacaagtt gcgacatcgg caaccaaagc gatacgcaca
    10861 taaccttccc cgggattgcc ctgttcggta tcccgtgcca aaaagcgtcc gggcaatact
    10921 tggatagcgg ctttttgcca taaattgcgt gcaaatgcca aatcgtcgcc atcggggact
    10981 ttcaaccaaa tgtaaaacga ggcatctggt aatttaacat caaatacctg ttgcaaaatg
    11041 ggaataacgc gctcaaattt ttcctgatac aggcggcggt tgtcgataac gtgctgttcg
    11101 tcatcccaag cggcaatgct tgcgcgctgc acgggaatgc tcattgcact gccgtgatag
    11161 gttctgtaaa gcagaaagtt tttaagcagt tcggcatcgc cggcgacaaa accggaacgc
    11221 aggcccggaa cgttggaacg cttggacaaa ctggtgaaca taagcagttt ttgcctgctt
    11281 cgacccaact gtgcagcggc ctgcaggcag cctaaaggtt tgttgccgtc gaaatagatt
    11341 tcggaatagc attcatccga ggcaataatg aaaccatatt tatcctgtaa atcaaaaact
    11401 tctttccatc catccaaatc cagcacgctg ccgctggggt tgttgggcga gcagacgaac
    11461 accagtttgg tgcgtttcca aacctcttcg gaaatactac gccaatcggg gttgaaagac
    11521 ggcgcggggc aattggcaaa atggatttca ccgccgccca aaagtgtcgc accttcgtaa
    11581 atctgataaa agggattcgg gctgacaatt gcgggtttga tgccgtctga aacagggtcc
    11641 aacacggttt gaacaaaaga aaacaacgcc tccctactgc ctaaaaccgg cagaatttca
    11701 ttatccgcat ccactgtcaa gccatcataa cggcgtttta accagttcgc acacgcctga
    11761 cgcagttcag gcaaaccggc cgtcagcgga tatttttcca actcgtgcaa tgaggcggtc
    11821 agcgcatctg taatgacttt cggtgttgga tgtttcggtt cgccgatatg cagtgggacg
    11881 gcttccagac cttcgggggc ggaaatgccc tgcatcgctt cacgcagtcg ggcaaaggga
    11941 tagggtttga gttggttgag taaggtattc ataatgttct ccggttgtcg gtatatcgga
    12001 agatatgccg tctgaacctg acggcaccca tcagacggca ttttatattc aatgcaaacc
    12061 cgtactgccg aaaccgcctt ctccacggct gcttccgaca aattcttcga cacgtttgaa
    12121 acgcgcctgc acgattggaa cgataaccat ctgcgcgata cgctcaaacg gcttgacggc
    12181 aaacggttcg ctgcttctgt tccataacga caccttcaat tccccttgat aatcggagtc
    12241 aatcaaaccg accaaattgc ccaaaacaat gccgtgttta tgccccaagc cggaacgggg
    12301 cagcaaaacg gcggcatatg aaggattggc caaataaatt gccaaacctg tcggcacaag
    12361 aaacgtttcc cccggctgca agacgacttc ctcatccaaa caggcgcgca agtccaaacc
    12421 ggcagaaccc gctgtcgcat aggtagggat aaaatccgcc atcctctcgt ccaatacttt
    12481 catttctact tcaatattca tcgtcttgct cctgcatacg gccggtttca aagatgggag
    12541 attatacaca agccatgttt acgctgaaac acagcaaatc ggttttgttt tgaaatccgt
    12601 tgccaatgta taatacccgc ccttccggtt atgaagacga acattatgca cacactaagg
    12661 caacttatca gaaatgactg aaactcaaaa aactggattc ccactttcgt gggaatgacg
    12721 tggtgcaggt ttccgtacga atggattcgt cattcccgcg caggcgggaa tctagacctg
    12781 tcggtttcgg tttttttggc tagtgccgca acattaaatt tct
//
LOCUS       DAXHPG010000043        12309 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXHPG010000043 DAXHPG010000000
VERSION     DAXHPG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.043"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(16..852)
                     /locus_tag="ACFM6F_000921"
     CDS             complement(16..852)
                     /locus_tag="ACFM6F_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC6164362.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLDLRLINGVEISVTWRGRTIHVVGLDFDERDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIRAGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGLLASAGSDFHRLNDFSGGILGACPELPENCRPVWEHFS
                     RH"
     gene            complement(1160..1561)
                     /locus_tag="ACFM6F_000922"
     CDS             complement(1160..1561)
                     /locus_tag="ACFM6F_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164363.1"
                     /translation="MAIYDLNEIVTSAALGMYKGKWQNMDFEQVLQDIVQETDFTYSW
                     EYGENWAIFCLNEKSVGMLCKQIPFAIIENEKLSQKIYQEYPWILTQQVYHFDTDKIL
                     LNKYVCKLTIGIMSAISQEPISLQDLYVDTV"
     gene            complement(1666..1869)
                     /locus_tag="ACFM6F_000923"
     CDS             complement(1666..1869)
                     /locus_tag="ACFM6F_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC6164364.1"
                     /translation="MDTTLKNKAEAILGEPLLDEPIRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(1847..2080)
                     /locus_tag="ACFM6F_000924"
     CDS             complement(1847..2080)
                     /locus_tag="ACFM6F_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6164365.1"
                     /translation="MEAIVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEE"
     gene            2618..6052
                     /gene="dnaE"
                     /locus_tag="ACFM6F_000925"
     CDS             2618..6052
                     /gene="dnaE"
                     /locus_tag="ACFM6F_000925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC6164366.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLVAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSSDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVRDKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQMARERYARSLSLALAPHHDIGKLVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            6095..6349
                     /locus_tag="ACFM6F_000926"
                     /pseudo
     CDS             6095..6349
                     /locus_tag="ACFM6F_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6486..7418)
                     /locus_tag="ACFM6F_000927"
     CDS             complement(6486..7418)
                     /locus_tag="ACFM6F_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6164367.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEMPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMPNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFSAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLNGIDVCLNIGALERGSGCKRREERDMAGLSERMAAELDRHG
                     AAVFFREYPNADHGNVSFYSLTDCVEYLRKAWQR"
     gene            complement(7488..9644)
                     /locus_tag="ACFM6F_000928"
     CDS             complement(7488..9644)
                     /locus_tag="ACFM6F_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFC6164368.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVCKRPAKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRHETFYAAADWDINPDTVLGAGYLYQQRHLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMHSHDVFADLKHYFGNGGYGKVGMRYSDRDADSNYAFAGSKLGMKT
                     PAGRPGCNTADDKACAVGLGTEIKQKALAFDASYSRPFRLGNTANEFVIGADYNRFRS
                     TNEQGRTTLYARGGLALNEFRSIPQVDLIANARKGVRGYSHTVATENLDEFGIYGKST
                     FHPADGLSLIGGGRLGHYKIESGEGKTLHKASKTKFTGYAGAVYDLNDNNSLYLSLSQ
                     LYTPQTNLDADGKLLKPRQGNQFEVGYKGSYMDDRLNARVSFYRMKDKNAAAPLNPNN
                     KKTRYAALGKRVMEGVETEISGAVTPKWQIHAGYSYLHSQIKTASNSRDDGIFLLMPK
                     HSANLWTTYQVTPELTIGGGVNAMSGITSSAGMHAGGYATFDAMAAYRFTPKLKLQIN
                     ADNIFNRHYYARVGGANTFNIPGSERSLTANLRYSF"
     gene            9804..10463
                     /locus_tag="ACFM6F_000929"
     CDS             9804..10463
                     /locus_tag="ACFM6F_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC6164369.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRDLLEMPSETAIADITRTYSAHYLNPNNRNMTLFPDALPCLDKLKAQ
                     GYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGFDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDGLSELPGFLAQHY
                     A"
     gene            complement(10550..11518)
                     /gene="ispH"
                     /locus_tag="ACFM6F_000930"
                     /gene_synonym="lytB"
     CDS             complement(10550..11518)
                     /gene="ispH"
                     /locus_tag="ACFM6F_000930"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC6164370.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAAGIGTDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     QEVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(11548..12042)
                     /gene="lspA"
                     /locus_tag="ACFM6F_000931"
     CDS             complement(11548..12042)
                     /gene="lspA"
                     /locus_tag="ACFM6F_000931"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC6164371.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     GKKT"
BASE COUNT         2792 a         3427 c         3256 g         2834 t
ORIGIN      
        1 catttttatc cgcattcaat gccgggaaaa atgttcccac accggcctgc agttttccgg
       61 cagctcggga cacgcgccga ggataccgcc gctgaaatcg tttaagcggt ggaaatcgct
      121 gcccgcgctt gccaacaatc cgaagcgttc tgccaaaagc gcgtagttga ggcggtcgtt
      181 tttgcaacag ttgccgctgt ggacttcgat gcctgcgccg ccgaggtttt taaattcttc
      241 aaacagattg cgcttggcgg tggcggacaa atcgtagcgc atcggatggg cgatgaccgc
      301 cagcccgccc gcgccgttga cggcggagac gcagtcttcc agcgtcgccc attcgtgtcg
      361 gacggcgcag gatttgccgt cgcccaagta tttggtgaac gcctgctgct tgtttttgac
      421 atgtcccgcc cggatgagga actcggcgac gtgggtgcgg ctgaccattt ctttgtttgc
      481 cgccagcgcg agcgcgccgt cgtatgcgcc gccgatgcct ttcttttcga gcttggcggc
      541 gatggcttcg aggcgtttca gacggccttg ccgcacttgc gccaacaggt tttgcaggtt
      601 ttcgtcccgt tcgtcgaaat ccaaaccgac aacgtgtatg gtgcgtccgc gccaagtgac
      661 ggagatttcc acgccgttaa tcaggcgcaa atcgagcctg tcggcttcgg cacgcgcttc
      721 ggcgatgccg ccggtgtggt cgtggtcggt caacgccagc agcgtgcagc cgttttgatg
      781 cgcgaggcgg acgacttcgg cgggggaaag cataccgtcg gaaacggtgg aatggcagtg
      841 caggtctatc atggggtgtt atatgtgtgg tgaatgaagg tcggggggca atattgtcgg
      901 gcggtgtggc ggcggcggtc attttaaaaa acaggtgtat ggcaaatgca aaggaaaagc
      961 cgctatgccg tcattcccaa cccccgaatg tgcggataga gccagtgggg atgggtacat
     1021 tatttgtttg cattagttag ttatcctgct gtatccatta aatcatatgc ttcaattaat
     1081 tgttgttcta gctcttgtac caattttgga ttgcgaattc ctaactcaat ttcaaacagc
     1141 gagttctcac tatgtacatc tacacagtat ctacataaag atcttgtaaa gatataggtt
     1201 cttggctgat ggcactcatt attccaatag ttaatttgca tacatatttg tttaataaaa
     1261 ttttatctgt atcaaaatga taaacttgtt gagttagtat ccaaggatat tcttggtata
     1321 ttttctgtga taatttttca ttctctataa tcgcgaaagg tatctgttta cataacatac
     1381 caacagactt ttcgtttaga cagaaaatag cccaattttc cccatattcc caagagtatg
     1441 taaaatctgt ttcctgaaca atatcttgta aaacttgctc aaaatccatg ttttgccatt
     1501 tacctttata catacctaat gcggcactgg ttactatttc gtttaagtca taaatagcca
     1561 tttatgttcc ttgctaattt ccaggtgaga catgtatagg actgagacca gtatgcccat
     1621 taggtttccc tctatacaca ttaatagtag atgttggttc cgttttcaga cggcatcgtc
     1681 cgaccaaccc gcttctttta atttccgccg ttgcgcgtcg tagcgggcct tatccgccca
     1741 ataaatcgtc tgaatgcacg cctcgccgca gtcgctgccg cagcattccc acgactcggg
     1801 gcggataggt tcgtctaaaa gcggttcgcc caagatggct tcggctttat tcttcagggt
     1861 cgtatccatc ggcaatttcc aagcgtgcgc cgtcaaactc gatgacttcg ccgccgcgta
     1921 ttttggcggt tttacgggtt tcggtttcgc cgttgcgcga caccaaccct tcggcaatca
     1981 acgccttcgc ctgtccgccg ctttcggcaa gtccggccaa tttcaacagg tcgcacaagg
     2041 cgatgtattc gttgtcttcg agatagacga tggcttccat gatgtcccct tgcagaaaga
     2101 gaccgttatt gtagcacctg ccgccgccgt acccaaaatt accgcaaaac cagcgatgta
     2161 tccgcaccgc ctgttccgta aaagtaaaaa tgccgtctga aaccccgtat gccgcaatcc
     2221 gttcaaagaa atcctgccca acggcagact gcaaatcctg ttccccgacg aatccgcatt
     2281 gacgctgatg cacatcctca aacacgaact gcccgataca ccggcaatcg gcatcaaaac
     2341 gaaatcaaaa acctgtttga acgtaaaatt aatttaactg cttgccatcc gtccgaataa
     2401 ggtatatagt tctttataac tagtttgata gttctttata tctatcaata ctccttggga
     2461 agcctccacc acacggtagg aggcattttt ttgccgtcgt aaaagctcaa aaacatttgc
     2521 aggtcatgcc gtctgaatcc gaaacagcat tacctacacc gccatctaaa gacaaccctg
     2581 ctacaatacg ccttttattg ttcacgccga ttttgccatg accgagccga cctacatccc
     2641 cttgcgcctg cataccgagt tctcaattac cgacggtatg gtgcggatta aaaaactggt
     2701 tgccaaagcg caggaatacg gtttgcctgc tttgggcatc agcgatttga tgaacgaatt
     2761 cggcttggtg aaattctata aagcctgccg cggcgcaggg attaaaccca tcggcgcggc
     2821 ggacgtgtgg ataggcaatc cgaatgcgcc cgacaagccg tttcgcgcca tgctgattat
     2881 ccgcaacgat gcgggctatc tgcgcttgag cgagcttctg acggcggctt atgtcggcaa
     2941 agaccgcaat atccatcatg cggaactcaa tcccgaatgg ctggaaaacg gcgacaacag
     3001 cggcttgatt tgtttgagcg gcgcacatta cggcgaagtg ggcgtgaatc tgttgaacgg
     3061 caatgaagac gcggcgcgtg cggcggcgtt gaaatatgcg gcatggtttc ccgatgcgtt
     3121 ttatttagag ctgcaacgcc tgcccgaacg ccccgaatgg gaggcttgcg tttccggcag
     3181 cgtgaagctg gcggaggaac tgggtttgcc ggtggtggcg acccacccga cgcagtttat
     3241 gagcagcgat gatttcaacg cgcacgaggc acgggtgtgt attgcgggcg gctgggtgct
     3301 gacggacaaa aaacgtccgc gcgatttcac accgggccag tttttcattc cgccggaaac
     3361 gatgctggag cgtttcgccg atttgcccga ggccttggaa aacacggtag aaattgccaa
     3421 acgctgcaat ctgcacatca cattgggcaa aaacttcctg ccccttttcc ccacgcccga
     3481 cggtttatcg cgcgatgact atctcatcaa actctcaaac aagggcttgc aggaacgcat
     3541 ggttcagctt tatcccgacg aggcggagcg tgcggcaaaa atgccggaat atcaggaacg
     3601 tttggatttt gagctgaaca tcatcatcca gatgaaattc cccggctatt tccttatcgt
     3661 acaagacttt atcaactggg cgaaaacgca cggctgtccg gtcgggccgg gacgcggttc
     3721 gggcgcgggt tcgctggtgg cgtattcgct gaaaattacc gacctcgacc cgctgaaata
     3781 cgcgctgctg tttgaacgtt tcttaaaccc cgaacgcgtc tccatgcccg acttcgacgt
     3841 tgacttctgt caggcaaacc gcggccgcgt gatcgaatat gtgcgcgata aatacggcgc
     3901 tcaggcggtc agccagattg ttaccttcgg cacgatgtcg tccaaagcgg tcatccgcga
     3961 cgtggggcgc gtgttggagc tgccgtttat gctgtgcgac aaactgtcca agctgattcc
     4021 gttggaagcc aacaaacctc tgggtttgga cgacgcgatg aaggcggagc cgcagattca
     4081 ggaattaatc gaagcggaag aagcggacga actgattacg ctggcgaaaa agctggaaga
     4141 cctgacgcgc ggtttgggta tgcacgcagg cggcgtgttg attgcgccgg gcaagatttc
     4201 cgattacagc cccgtgtatc aggcggatga atccgcctct cccgtatcca tgtacgacaa
     4261 gggcgacgtg gaagacgtgg gtttggtgaa attcgacttt ttaggtctgc gtaacctgac
     4321 cattatcgaa atggcgcaga acaacatcaa aaacaccacc ggcgacatca tcgatgtcgg
     4381 caaaattccg ctcgacgacc aagccgccta ccaaatcttc cgagatgcga acaccaccgc
     4441 cgtgttccag ttcgagtcga ccggcatgaa aaaaatgctg aaaacggcgc acacgaccaa
     4501 gtttgaagaa ctgattgcct tcgtatcgct ctaccgcccc ggcccgatgg acaacatccc
     4561 cgacttcgtc gcgcgcatga aaggccagga attccaatac atccatccgc tgctcgaagg
     4621 catcctcgcg ccgacctacg ggattatggt gtatcaggaa caagtgatgc aggcggcgca
     4681 aattatcggc ggctactcgc tcggcggcgc ggacctgctg cgccgcgcga tgggtaagaa
     4741 aaagcccgaa gaaatggtga aacaccgcga aatctttgcc gaaggcgcgg caaaacaagg
     4801 catttcgcgc gaaaaatccg acgaaatctt caactacatg gaaaaattcg ccggctacgg
     4861 tttcaacaaa tcccacgccg ccgcctacgc cctgatttcc taccagaccg catggcttaa
     4921 agcccactat cccgccgaat ttatggcggc gaccatgtcg tccgaattgg acaacaccga
     4981 ccagctcaag catttctacg acgactgccg cgccaacggc atcgagttcc tgccgcccga
     5041 catcaacgaa tccgactacc gcttcacgcc gtatccggac atgaaaatcc gctacgcgct
     5101 cggcgcgatt aaaggcacgg gcgaagccgc cgtcgaatcc atcatcgccg cgcggcaaag
     5161 cggcggcaag tttaccggcc tcttggactt ctgcgagcgc gtcggcaaag aacacatgaa
     5221 ccgccgcacc cttgaagccc tgatacgcgg tggcgcgttc gacagcatcg aacccaaccg
     5281 cgccatgctc ttggcgaaca tcgacctcgc tatggacaac gccgaccaaa aagccgccaa
     5341 cgccaatcag ggcgggcttt tcgacatgat ggaagacgcc atcgaaccgg tgcggctcat
     5401 cgacgcgccg atgtggagcg aatcggaaaa actcgccgaa gaaaaaaccg tcatcggctt
     5461 ttacctgtcc ggccacccgt tcggcccgta tgcccaagaa gtccgccaaa tcgccccgca
     5521 gaaactgtcg aaactcaaac cgcaagacag cgtgcgcctc gccggattcg ttaccgccgt
     5581 acgcaccatg atgggcaagc gcggcaaaat cgccttcgtc agccttgaag atttgagcgg
     5641 acaggttgaa atcatggtcg gcggtcagac gttggaaaac tgcgccgact gcctcaaagc
     5701 cgaccaagtg ctgattatcg aatccaaagt cagccgcgac gactacggcg gcggcgacgg
     5761 gctgcgtatt ctggcaaacc aagtcatgac cctgcaaatg gcgcgcgaac gctacgcccg
     5821 aagcctcagc ctcgccctcg ccccgcatca cgacatcggc aaactggtac aactgctcgc
     5881 cgcccaccaa ctgcccgaca cgccgcgcat cccgctgcaa ctgtcgtatg ccaacgaaaa
     5941 agcgtcgggc aggcttcaag tgccgccgaa atggacggtt acgccgagtt ccgcattgtt
     6001 cggcgaactg gaaacattgc tcggcagccg gtcggtgcgc gtcaactggt aacccaaaat
     6061 ataaatgccg tctgaagccc aaaaaccggt ttcattcgta ctttattcga atgattgaat
     6121 aaaagtaact gccaagaaaa acgtattttt tggttatttc gccagtctaa atagagcaac
     6181 cgggacgatt gatatccgtg tgcatgacac agacagcacc aaagggaaaa acggcatttt
     6241 ccaaagtatc ggtatcaaaa ccgccctttc actccaaaaa taccaaatcg acaaaccggg
     6301 caaagaaatc agaccgtgcc gcctgaaaaa acgcccaccc gtccgttaaa ttaacctatc
     6361 cctgtttcag acggcctgaa gcagggattt ttatatcaaa ataaaatgag aaagggagca
     6421 ataaccctta ggtagctctt gttattttcc gatgcaaaac aaagcagtca tatatttaat
     6481 tccccctacc tctgccaagc cttcctcaaa tattcgacgc aatcggtcag cgagtagaac
     6541 gagacattgc cgtggtcggc attcggatat tcccggaaaa agacggcggc cccgtgcctg
     6601 tccaactctg ccgccatgcg ttcggacagt cctgccatat cgcgttcttc cctgcgtttg
     6661 caaccgctac cccgttcgag cgcgccgatg ttgaggcaga catcgatgcc gtttagccgg
     6721 ttttcagacg gcataaaatc gagtatccgc ctgttgtgcc accaaatcga tggggatacg
     6781 agccaatgcc gtctgaaacg gcggtgggaa agcagggaat acagtccgaa cagtgcgccg
     6841 aacgagtgtc cgaatacggc ggtttcattg cggttgaggg tgtagcggct ttctaaaaag
     6901 gcggtcagct cgctgtcgat aaaggcggag aagcggtctg cctgtccgaa ctgctgccgt
     6961 tcgtctgctg tggcgttgtc tccaagcggc ggcgtgtagt cggcggcacg ttgtgccaaa
     7021 tcgcgcacac tgcctgtcgt gtagccgata ccgacaatca ggcagggggc gttgcttcgg
     7081 gtaacggggt tgggcatcag cgactgcatg atgttgaaaa gtgcggggaa aaaggcttcg
     7141 ccgtccaaaa cgaagaggac gggatagcct tcagacggca tttcgccgag tgtcgccgtc
     7201 tgaatccgat agattcgccc cgtgcaggtg gatttgattt cggtttcaaa ggcttggggc
     7261 agtatggcag gttggaatgt gtcggtcggt atgggtttca tgatgttcgg cttgtgggtc
     7321 agactggcgg caagcggtaa aaccgaaccg gcaaagcggt ctgcctgccg gttcggtctg
     7381 ttttccttcg caatgccata ctccagttgt gagagcatag ggtatgcagc gcagcttgtt
     7441 gtggtttgat gatgctgcgg ctgccgtttc agacggcata ttggtcttta aaaactgtaa
     7501 cgcaggtttg ccgtcaggct gcgctccgaa ccgggaatgt taaaggtgtt cgcgccgccg
     7561 acgcgggcgt agtaatggcg gttgaagatg ttgtcggcgt tgatttgcag cttcagcttg
     7621 ggcgtgaagc ggtatgccgc catcgcatcg aacgtggcat aaccgcctgc atgcatccct
     7681 gcagatgaag taatgccgct catcgcgttc actccgccgc cgatggtcag ctcgggcgta
     7741 acttggtaag tcgtccacag gtttgcgctg tgtttgggca tcaataggaa gatgccgtcg
     7801 tcgcgtgaat tggaggcggt tttgatttgg ctgtgcagat agctgtaacc tgcatggatt
     7861 tgccatttcg gtgtaaccgc gccgctgatt tcggtctcaa cgccttccat cacgcgtttg
     7921 cccaatgcgg cgtaacgggt ttttttgttg ttcgggttca acggtgcggc ggcgtttttg
     7981 tctttcatgc ggtagaacga aactcgggca ttgaggcggt cgtccatgta gctgcctttg
     8041 taaccgactt caaactggtt gccttggcgc ggtttgagca gcttgccgtc ggcatcgagg
     8101 ttggtttgcg gtgtgtagag ctgggacagg ctcaggtaga ggctgttgtt gtcgttcaag
     8161 tcgtaaaccg cgcctgcgta gccggtgaac ttggttttgc tggctttgtg cagggttttg
     8221 ccttcgcccg actcgatttt atagtgtccc aaacgtccgc cgccgataag cgacagcccg
     8281 tcggcaggat ggaaggtgga tttgccgtaa atgccgaatt cgtcgaggtt ttcggtagcg
     8341 acggtatggc tgtaaccgcg cacgcctttg cgcgcgttgg caatcaaatc aacctgcggt
     8401 atgctgcgga actcgtttaa agccaggccg ccgcgtgcat ataaagtagt acggccttgt
     8461 tcgttggtgc tgcggaagcg gttgtaatcg gcgccgatga caaattcgtt ggccgtattg
     8521 cccaagcgga aaggcctgct gtagctggcg tcaaacgcga gggctttttg tttgatttct
     8581 gtacccaaac ccaccgcgca ggctttgtcg tcagccgtat tgcagcccgg gcggcctgcc
     8641 ggggttttca tgcccagctt gctgccggca aaggcatagt tggagtcggc atcgcggtcg
     8701 gaatagcgca taccgacttt gccgtagccg ccgttgccga agtaatgttt caaatcggcg
     8761 aacacgtcgt ggctgtgcat tttaaatttg ttccaatccg cgccgacaaa tacgtgttgc
     8821 ggcagggacg gtaatttgtt attggcatcg gctggcaagc cgttgtacgg cgcgaggtgg
     8881 cgttgctggt aaagatagcc cgcgcccaaa accgtatcgg ggttgatgtc ccaatccgcc
     8941 gccgcgtaga aggtttcgtg ccggttgttt ttctcggcgg gacgcggaga cgcgccgacg
     9001 gtctgcgcca tcacgcggcc gcgcacgctg ccgtctgaat tgaggctgcc cgatacgtcc
     9061 gcctcggctt tatattgttt gtgcgtaccg aaccctgccg cagcatgacc ttggaacgct
     9121 ttggccgggc gtttgcacac cagatttacg atgccgccca tctcgccgct gctgtcgaac
     9181 aagccgctcg gcccgcgcat cacttccaca cggtcgaagg caaacagatt gggcagcgtg
     9241 ccgttgatac tctgcatctg cgcgggcagg ccgtcgatgt tgtattcgct gtattcgtaa
     9301 ccgcgcgcgt aaaccgaaga gcgtccgtcg tcgttgctca acacgcgcag gccgggcgtt
     9361 ttgcgtgcca actggtcaaa cgtatcaaca ttgcggtctt tgacctgttg attggtgatg
     9421 atgctgacag attgcggaat ttcgcgcaaa gaagcgggga tttttgtgcc gacggtggcg
     9481 gcaaacgagc tgtaatcgcc gtttttctcg gtggcaatcg cgttgtacga acgctgaccc
     9541 ttaatatgga cggtttccaa atccgccgtt tgtgcggcaa aaaccgaaga cgaaagtgct
     9601 gccaaaaccg tggcggcggt catattgatg cggaaaactg acataaactg tcccattcac
     9661 ataaatgata atggttctat tttaataaag cacaacgcgg cttgttcgga aaaacatatc
     9721 gcgcagccga caaattttgt cgaaaatgcg acacgtctgc gttttccgca taaaatctga
     9781 cttttcaccg taaacaacct gccatgacca cgcccaaact catcatcttc gactgggacg
     9841 gcacgcttgc cgatacgacc caacccatca tcgacaccat gcgccgcagc ttcgccgaat
     9901 gcggttttcc gccgcccgaa gcggaacgcg tccgcagcct gattggctac agcctgcccg
     9961 aaatcatccg cgacctgctc gaaatgccgt ctgaaaccgc cattgccgac atcacacgca
    10021 cttattccgc acattacctc aatcccaaca accgcaatat gacgctcttt cccgatgccc
    10081 tgccctgtct ggacaagctc aaagcacaag gatactggct tgccgtcgcc acgggcaaag
    10141 ggcgggcggg tttggacaac gccatcagcc aaaccgccac cggcggctat tggctcgcca
    10201 ccgcctgcgc gggggaatat ccctccaaac cctcgcccga aatggtattc ggaatctgcg
    10261 gcgaactggg gttcgacccg aaagaggcat tggtcgtcgg cgatacggcg catgacctgc
    10321 atatggcggc aaacgcaggc gcggcggcag tcggcgttgc caccggcgca cattcgcgcg
    10381 aacagctcct tagcgcaccg catctcgccg tattggacgg tttgtccgaa ctgcccggtt
    10441 ttcttgcaca gcattacgcc tgattggttt ccgcatccgg cactcgaaac aaatgccgtc
    10501 tgaaggttgt tcagacggca tttgttttgc ccaaacattc aacgcttgct caacgtttgc
    10561 acaaatcggg tttggtttcg ccctcgcggc gcaactcttt gggcaggacg aacacgatgc
    10621 tttcttccgc gccctcccct tcgcgtacgg tttcatgccc ccacccgcgt atggttgcca
    10681 atacttcctg caccaacact tcgggcgcgg acgcgcctgc cgttacgccg actttgttct
    10741 tgccctcaaa ccacgcgcgt tgcaggtagc ctgcattgtc caccatatac gcatcggttc
    10801 cgatgcctgc cgccacttcg cgcaggcggt tgctgttgga cgaattgggc gaaccgacca
    10861 caatcacgat gtcgcactgt tctgccaact ctttgacggc ggtttgccgg ttggtcgtcg
    10921 catagcagat atcttccttg tgcggattgc ggatattggg gaaacgcgcg ttcagcgcgg
    10981 cgatgatgtc tttggtttca tcgaccgaga gcgtggtttg gctgacatag gcgagtttgt
    11041 cggggtttct gacttcgagt tttgccacat ctccgaccgt ttcgaccaaa agcattttgc
    11101 ccggcgcaag ctgccccatc gttccttcaa cctcgacgtg ccccttatgc ccgatcatga
    11161 tgatttcaca gtcttgggca tccagtcggg cgacttcctt atgcactttc gtcaccagcg
    11221 ggcaagtcgc atcaaacacg cggaaaccgc gctccgccgc ttcttgccgc accgccttcg
    11281 atacgccgtg tgccgaataa accagtgtcg cgcccggcgg cacttccgcc aagtcttcaa
    11341 taaacaccgc acctttttca cgcaggttgt ccacgacgaa tttgttgtga acgacttcgt
    11401 ggcgcacata aatcggcgcg ccgaattctt ccaaagcacg ttcgacaata ctgattgccc
    11461 gatccacgcc ggcgcagaag ccgcgcggat tggcaaggat gatggttttc tcgttcataa
    11521 gttttgcatt ccgtgttcag acggcattca cgtttttttg ccatctttgc gatggacgat
    11581 attgtcaagc actgccaata ccgcgccgac gcagataaaa ctgtcggcaa tattaaaggc
    11641 gggataaaac caattttgcc aataaaacaa taagaaatcg acgacatgac cgtgtatcag
    11701 gcgatcgatg acattgccta acgcaccgcc gataatcatc gccgcacccg ttttgccgag
    11761 ggctgcaaac tcatcgcgca agatggcgcg taccaaatac gcgctcaccg ccaccgccag
    11821 caccaaaaaa aagtattttt gccagccgcc ctgatcggca aggaagctga acgccgcacc
    11881 cgggttgtac accagcgtca gatcgaaaaa ggaaggaatg acattgacgc gttcccgata
    11941 ctgaaacgac gacagcaccg cccacttcga ccactggtcc agcacgatgg cggcaagtgc
    12001 caatacccaa tagcgcgttt tacttgaaac agatgaagac atatttttca acagccggta
    12061 aaagagtacc attttacccg aaaaccccct ttcctgtacc cgaaacggca aatgccgtaa
    12121 tcttaaaacc cgtcattccc gacaacaccg taatctcgaa acccgtcatt cccgcgtagg
    12181 cgggaatcca gacctgtccg cacagaaact tatcggataa aaacagttgc tcaaacccct
    12241 cgttctagat tcccactttc gtgggaatga cggttcagtt gcgttccaac aacaccgcaa
    12301 tctcgaaat
//
LOCUS       DAXHPG010000044        12235 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXHPG010000044 DAXHPG010000000
VERSION     DAXHPG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12235
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.044"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            246..983
                     /locus_tag="ACFM6F_000932"
     CDS             246..983
                     /locus_tag="ACFM6F_000932"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC6164372.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGCELIRED"
     gene            986..1672
                     /locus_tag="ACFM6F_000933"
     CDS             986..1672
                     /locus_tag="ACFM6F_000933"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC6164373.1"
                     /translation="MAGLTFEQTVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1831..2694
                     /locus_tag="ACFM6F_000934"
     CDS             1831..2694
                     /locus_tag="ACFM6F_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6164374.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2756..3325)
                     /locus_tag="ACFM6F_000935"
     CDS             complement(2756..3325)
                     /locus_tag="ACFM6F_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC6164375.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3483..4343
                     /locus_tag="ACFM6F_000936"
     CDS             3483..4343
                     /locus_tag="ACFM6F_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC6164376.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            4479..4817
                     /locus_tag="ACFM6F_000937"
     CDS             4479..4817
                     /locus_tag="ACFM6F_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164377.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            5185..5538
                     /locus_tag="ACFM6F_000938"
     CDS             5185..5538
                     /locus_tag="ACFM6F_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC6164378.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5528..6394
                     /gene="atpB"
                     /locus_tag="ACFM6F_000939"
     CDS             5528..6394
                     /gene="atpB"
                     /locus_tag="ACFM6F_000939"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC6164379.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6451..6687
                     /gene="atpE"
                     /locus_tag="ACFM6F_000940"
     CDS             6451..6687
                     /gene="atpE"
                     /locus_tag="ACFM6F_000940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC6164380.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6758..7228
                     /locus_tag="ACFM6F_000941"
     CDS             6758..7228
                     /locus_tag="ACFM6F_000941"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC6164381.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7233..7766
                     /locus_tag="ACFM6F_000942"
     CDS             7233..7766
                     /locus_tag="ACFM6F_000942"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC6164382.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAIIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7777..9324
                     /gene="atpA"
                     /locus_tag="ACFM6F_000943"
     CDS             7777..9324
                     /gene="atpA"
                     /locus_tag="ACFM6F_000943"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC6164383.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9349..10224
                     /gene="atpG"
                     /locus_tag="ACFM6F_000944"
     CDS             9349..10224
                     /gene="atpG"
                     /locus_tag="ACFM6F_000944"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC6164384.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10262..11659
                     /gene="atpD"
                     /locus_tag="ACFM6F_000945"
     CDS             10262..11659
                     /gene="atpD"
                     /locus_tag="ACFM6F_000945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC6164385.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRAIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11670..12092
                     /locus_tag="ACFM6F_000946"
     CDS             11670..12092
                     /locus_tag="ACFM6F_000946"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC6164386.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTILADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         2996 a         3137 c         3160 g         2942 t
ORIGIN      
        1 ttttcaatta tttgccgttt tggtgcgaac cgctgccttt gcccgtttca gacggcattg
       61 tccgaaatgg ttgcctgctt cctgccttat tgacaaaaaa atgctttccc gataatatcc
      121 tacgaaaatt aacctgccga tttgacacag cttgcgggca taacagctaa agcgttccga
      181 caattccagc tttatcttcc gcgcccgttg tgtccgacat cgggctttgt tgtatgggaa
      241 agacaatgat tattttggac aaggtttcca agcattacca aacgcgcgac aagacccgtt
      301 ttgccgccgt cgagccgacc agcctcgaaa tccgcgacgg cgaaatcttc gggctgatgg
      361 gctattccgg cgcgggcaaa tccaccctgt tgcggctgat taacctgttg gaacgccccg
      421 acacgggcaa ggtcaacgtc tgcggacaag agctgaccgc gctcgatgcc gccgcattgc
      481 gtcaggctcg gcagaatatc ggcatggtgt ttcagcagtt caacctgttg aacaaccgca
      541 ccgtcgccgg caacgtcgcc ttcccattgg aaatcgccgg atggccgtct gaaaaaatca
      601 aagcgcgcgt tgccgaatgt ctcgaaatcg tcggactgac cgaacgcgcc ggccactatc
      661 ccgcccagct ttcaggcggg cagaaacagc gtgtcggcat cgcccgcgca ctcgcgccca
      721 aaccccaagt catcctcgcc gacgaaccca cttccgccct cgaccccgcc accacgcgca
      781 gcgtcttgga atgtttggaa gacatcaaca aacgattcaa cgtaaccatc gtcatcgtaa
      841 cccacgaaat gagcgttatc agacgactgt gcgaccgcgc cgccctgttg gataaaggca
      901 aagtcgtcga aatcgtcgaa gtacgcggca accaaatcca cgcccgatcc gacatcgggt
      961 gcgaactgat tcgggaggac tgaatatggc aggcttaaca tttgaacaaa ccgtttccaa
     1021 catcatcggt atgaaaggcg aaatcgtccg cgcattgggc gaaaccttcg taatggtcgg
     1081 tttgtccacc acattcgccg tcgttttcgg cacgttgctg ggcgtgttgc tcttcgtaac
     1141 ttccagccgc caactgcatt acaacaagcc ggtgaacttc ctgctcgaca acctcgtcaa
     1201 cctcatgcgc gccttcccct tcgtcatttt gatgattgcg atgatacccg ccacacgcgc
     1261 catcgtcggc agcaccatcg gcccggttgc cgcctcgctg gtgttgagcg tgtcgggatt
     1321 gttttatttt gcccgactgg tggaacaaaa cctgcgcgaa gtccccaaag gcgtaattga
     1381 agccgccgcc gcgatgggtg cgccgccggt tgccatcgtc cgcaaagtcc tcttaaacga
     1441 agcgcgcgcg ggcatggttt ccagcattac cgtgcttgcc atcgggcttt tgtcatacag
     1501 cgcggcggca gggatgatag gcggcggcgg cttgggcgac ctcgccatcc gctacggcta
     1561 ctaccgctac caaaccgaag tcatcgtctt catcgtcgcc ctcctcgtgc tgctggtcat
     1621 cctgattcaa agcaccggca acgcattggc gcggaaattg gataaacgtt aaacccgaat
     1681 gccgtctgaa cgccaaaacc cccaccgcta tccgaaaaat gctataaaat ccccctgttc
     1741 gcggcaaatg ccgtctgaac gccgaatctg gacggcagga ctccctgccc gtcatttttg
     1801 tttgaaactg ccacaacatc aggagaaaat atgaaaacct tcttcaaaac cctttccgcc
     1861 gccgcactcg cgctcatcct cgccgcctgc ggcggtcaaa aagacagcgc gcccgccgca
     1921 tccgcttctg ccgccgccga caacggcgcg gagaaaaaag aaatcgtctt cggcacgacc
     1981 gtcggcgact tcggcgatat ggtcaaagaa cacatccaac ccgagctgga gaaaaaaggc
     2041 tacaccgtca aactggtcga gtttaccgac tatgtgcgcc cgaatctggc attggctgag
     2101 ggcgagttgg acatcaacgt cttccaacac aaaccctatc ttgacgactt caaaaaagaa
     2161 cacaatctgg acatcaccga agtcttccaa gtgccgaccg cgcctttggg actgtacccg
     2221 ggcaagctga aatcgctgga agaagtcaaa gacggcagca ccgtatccgc gcccaacgac
     2281 ccgtccaact tcgcccgcgt cttggtgatg ctcgacgaac tgggttggat caaactcaaa
     2341 gacggcatca atccgctgac cgcatccaaa gcggacattg ccgaaaacct gaaaaacatc
     2401 aaaatcgtcg agcttgaagc cgcgcaactg ccgcgtagcc gcgccgacgt ggattttgcc
     2461 gtcgtcaacg gcaactacgc cataagcagc ggcatgaagc tgaccgaagc cctgttccaa
     2521 gaaccgagct ttgcctatgt caactggtct gccgtcaaaa ccgccgacaa agacagccaa
     2581 tggcttaaag acgtaaccga ggcctataac tccgacgcgt tcaaagccta cgcgcacaaa
     2641 cgcttcgagg gctacaaatc ccctgccgca tggaatgaag gcgcagccaa ataaggcagt
     2701 cgtataaaat gatgccgtct gaaccgtatc cgtgttcaga cggcattttt gtcctttaat
     2761 ccgccattcc ctgccattcc gccgaatccg gcgtatcgat tccgaacagc gacaaagcgt
     2821 gtgcaacact gtgcgccact atgtcgtccg ccgtctgcgg tttgcggtac atcgcaggaa
     2881 cagggggaaa caccacgccg cccatttccg ttacccgctt catattgtcc aaatgggcaa
     2941 ggttcagcgg cgtttcgcgc accatcagca ccagccgccg cctttccttc aaaaccacat
     3001 ccgccgcacg cgtcaagagg ttgtcgccga agccgtgcgc gaccgaggca agcgtccgca
     3061 tcgaacaggg ggcgaccagc atcccgtccg ttttaaacgt accgctggca atgcacgccc
     3121 cgatattgcc gatcggatgc acgaagtccg ccaaggcata tacctcatct ctcgcataag
     3181 ccgtttccga agcgcgcgcc atctccgcac ctttcgatac cacaaggtgc gtttcgatat
     3241 cttgcgcgcg taaaagttcc aaagccttca cgccgtattg gaaaccgctc gccccactga
     3301 tgccgattat caaacgccgt accatcatcc gcctttccca taaaaccgcc tgcaacggca
     3361 aaccggctat tatagtgaaa aaacagaaat ccgataaata cggatacaaa ttgtcggcaa
     3421 cacccaatat ccgataaaat atccgattta acatcctatc tgaataggca cgggagggcg
     3481 gtatggcaaa agtaaaaggc ggattggggc gcggtttgga ttcgctgctc gccaacggcg
     3541 cagacaacag cagcggcgac cgattgacca cggttgcggt taaagatatc cggcccggcc
     3601 gctatcaggc gcgtgttcaa atcgatgacg aagccttgca ggagctggcg gattcgatta
     3661 aggcgcaagg cgtgatacag cccgtcatcg tgcgcgaaca cggactgtcc cgatacgaac
     3721 tgattgcggg cgaacgccgt tggcgcgccg cacagattgc cggcctgacc gaaatccccg
     3781 ccgttatcaa aaccatcagc gacgaaaccg cattggcaat gggtttgatc gaaaacctcc
     3841 agcgcgaaaa cctcaacccc atcgaagaag cacaaggctt gaaacgcctt gccgacgagt
     3901 tcggactgac ccacgaaacc atcgcccagg ccgtcggtaa aagccgaagc gcgatttcca
     3961 acagcctgcg ccttttaagc ctgcccgaac ccgtgcagga aatgctttac caacgccgcc
     4021 tcgaaatggg acacgcccgc gcattgctga ccctgcccgt cgtcgaacag ctcgagttgg
     4081 cgcaaaaggc cgtcaaaaac ggctggtcgg tgcgcgaagt cgagcgccgc agccaagccg
     4141 cccttcaaaa caaacgtccc gagcccaaaa agactgccgc cgccgacatc ggccgcctga
     4201 atgatttgct gactgaaaaa ctgggtgtca acgccgaaat caaaaccgcc aaccataaaa
     4261 aaggcagaat catcctgcat ttcgacacgc ccgaaacctt cgaccatatc ttgaagcagt
     4321 tgggcataga ttaccgacct taattttgcg ggatataccg tctgaaatat agagaatagc
     4381 tttccagatt ttaagtggga aatataattc tattgacatt ttttctgctt cacgtaagaa
     4441 tcgttttcct gttttcattt ttaattttcg aggaaattat gaacacacgc atcatcgttt
     4501 cggctgcgtt cgttgcgttg gcattagcag gttgcggctc aatcaataat gtaaccgttt
     4561 ccgaccagaa acttcaggaa cgtgccgcgt ttgccttggg cgtcagccca aatgccgtaa
     4621 aaatcagcaa ccgcagcaat gaaggcatac gcatcaactt taccgcaact gtgggtaagc
     4681 gcgtgagcca atgctatgtt accagtgtaa tcagcacaat cggcgttacc acttccgatg
     4741 caatttgttt gggaggcgga acgcacaaag gcaaaagtca atgcaatgct ttgcttaaag
     4801 cggcaggccg ttgctaatcc tttattcgga aaaggtcgtc tgaaaatatt ttcagacgat
     4861 cttttattta ttgagcaaat tccccaaact gccgacaatc cgaccgataa actgctacaa
     4921 tttcgcgttg attttttatt atttccacgt aaagcaaaca aatcacgtaa gaacattaaa
     4981 agtgtaaaga aattgcggac aaactcctta atatcctgta attatatgta atgtgtgtta
     5041 gaatgacacc tgtcgccgtt attcaggcgg cgagcgcggc ggattgggat acgttgtttc
     5101 acaaagatta actgtcgctt gacgtaataa ggcttctaaa ttatattagc cgcgccccaa
     5161 atccgaggcg gaaacattcc ctttatgaag cagattatca tcctacaatc cgccgtattg
     5221 tccatatgcg ccgcagttgc ctttgctgta tggggttttg ccggattcct ttcggcggtc
     5281 ggtggcggtt tgtcctacct gcttcccacg tttgttgcag ttttactttt aaaacttttc
     5341 aggggaaacc ctttcctgca aagcaggatg ttcgtcttcg gagagatttt aaaagtagtg
     5401 ctgtcgctgt tgtccatgct cgccgtattt gctgtatggc atcaatcgtt ggtgttcgcc
     5461 ccgtttctga tggggctgct cggtgtcagc catttggttt ttttagtatt gttgagagtg
     5521 aaagattatg gcaggtgaaa ccattaccgc tgccgactac atcaagcacc acttgcagag
     5581 cttgaccagt ttgtcggatg ttactcaggg tcagggactg aaaaacattg ctgatttttc
     5641 gtttattaac cttgatgccg tcttttttgc cgtcctgttg ggcgtaatcg gcagcttcct
     5701 gttgtggcgc ggtgcgaaaa aagcaacggc aggcgttccc ggacgtttcc aggctgccgt
     5761 ggaaatcttg tttgagtttg tggacgatat gtgtaagagc atcattcaca acgaaaagtc
     5821 tagaaaagcc gtcgcgccgt tgggtctgac gctgtttgtc tggatttttc tgatgaacgc
     5881 gatggatatg ctgccggtcg atttgctgcc gatggtatgg cagggcatta ccggcaacca
     5941 tcacgccctg ctgcgcgtcg taccgactgc cgatttgaac accactttgg cactcgctgt
     6001 cggcgtgttg ctgatttgta tctattacaa catcaaaatc aaaggattgg gtggctggtt
     6061 tcacgagttg ttcagcgcgc cgttcggtgc aaaactcgca cctgcgaact ttctgttgaa
     6121 tttggtcgag tttctttcta aaaccgtatc ccacggtatg cggttgttcg gcaatatgta
     6181 tgcaggcgag ttggtattct tgttgatagc cttgcttggc ggtgcttggg cggcttccgg
     6241 cagcgtcgaa gtcatggatc cgattctgtt tgtattccac attattgccg gtttggcatg
     6301 ggcgattttc catattttgg tgattaccct gcaggcgttt attttcatgg cgttggcgtt
     6361 cgtctatatc ggacaggcac atgatgcgca ctgattttcc taagtagttt gtttttgtag
     6421 tttaaccttt gtttctttaa ggagtttaaa atgggtttga ttgctatcgc atgtggtttg
     6481 atcgttgcat tgggtgcatt gggtgcctct atcggtatcg caatggtcgg ttctaaatat
     6541 ttggagtctt ctgcccgtca gcccgaactg atcggtccgc tgcaaaccaa actgttcctg
     6601 attgccggtt tgattgatgc cgcattcttg attggtgtcg ccattgcact gctgttcgcc
     6661 ttcgttaacc cgtttgcagg tgcataatca ggacggattc gtccgttccg tacaggcgca
     6721 gcctgtttga ttaaccccag tacgaaggtt aagtaacgtg aatatcaatg caacattatt
     6781 cgctcaaatc atcgtctttt tcggtttggt atggtttacc atgaaattcg tgtggccgcc
     6841 gattgcaaaa gctttggatg agcgtgccgc aaaagtcgcc gaaggcttgg ctgccgccga
     6901 gcgtggtaaa agcgattttg agcaggctga aaaaaaggtt gcagaactct tggcagaagg
     6961 gcgtaatcag gtttccgaaa tggttgccaa cgccgaaaaa cgtgccgcca aaattgtcga
     7021 agaagccaaa gaacaggctt cttccgaggc ggcgcgcatt gcagctcagg caaaggccga
     7081 tgtggagcag gaattgttcc gcgcacgcga atccctgcgc gagcaggttg ccgtgttggc
     7141 tgtcaaaggt gccgaatcta ttttgcgcag cgaagtcgat gcttccaaac acgcaaaact
     7201 gctcgatacc ctgaaacagg agctgtaatc ttatggcaga gttcgcaacg attgccagac
     7261 cttatgcaaa ggcattgttc ggtctggctc aggaaaaaaa ccaaattgag tcttggttgg
     7321 gcggactgga aaaacttgcg gcggttgttc aggaagggaa ggtggcttca ttgattgacc
     7381 gtcctgaaac gaatgcttca gaaaaagcag atatcctcat cgatttggtc ggtttgaaag
     7441 acaaggagtt gaaaaacttt gttatcgtct tggccgggca gaaacgtttg tcgatattgc
     7501 cggaagtata tgctcaatat caagacttga ccttatcatt caaccatatc aaatctgcca
     7561 tcatttacag tgcctatccg ttgaccgaca aacaggtcgg cgagttggcg caaatgctga
     7621 ataagcgttt cgacagcgag ctgaaaatct ctgtcgaaat cgaaccggag ctgattggcg
     7681 gcataaaagt tgaagtgggt gatcaggttt tggatttgtc tgtacaaggc aaattgagtg
     7741 ccttgtacac gactatgacg aattaggaga gttttcatgc agcttaatcc tgctgaaatt
     7801 agcgatttga ttaaagccaa gatcgaaaat ctgtctgtaa atgccgaagt gcgtacccgt
     7861 ggtacggtga tttcggtaac tgacggtatc gttcgcatcc atggtttgtc agatgcaatg
     7921 caaggtgaga tgctcgaatt cccgggtaac actttcggct tggcgatgaa cctggagcgc
     7981 gactccgtcg gcgccgtagt gttgggcgag tacgaacata ttaaagaagg cgacacggtt
     8041 acctgtaccg gtcgtatctt ggaagtgccg gtcggacgcg aactggtcgg acgcgtcgtt
     8101 gatgcattgg gtcggcctat cgacggcaaa ggcccgatta atacaacttt gaccgcccct
     8161 atcgaaaaaa tcgcaccggg cgtgattgcg cgcaaatcgg ttgaccagcc gatgcaaacc
     8221 ggtctgaagg cgattgactc tatggttcct gtcggtcgcg gtcagcgtga gttgatcatt
     8281 ggtgaccgtc aaaccggtaa aactgccgta gcactggatg ccatcgtcaa ccaaaaaggt
     8341 acgggtgtta tctgtatcta tgtcgctatc ggtcaaaaag catcttctat tgccaacgtg
     8401 gtccgcaaat tggaagagca tggcgcgatg gaacacacga ttgtggttgc tgcaactgct
     8461 tctgaagccg ctgcactgca atacatcgca ccttactccg gttgtacgat gggtgaattc
     8521 ttccgcgacc gcggcgaaga tgccttgatt gtttatgacg atttgtccaa acaggctgtg
     8581 gcttaccgtc aaatttccct gcttttgcgc cgtccgcccg gccgcgaagc ctatcccggc
     8641 gatgtgttct acctgcactc ccgtctgttg gaacgtgcgg cacgtgtcaa tgaacacgaa
     8701 gtggagaaat tgaccaacgg cgaagtaaaa ggcaaaaccg gttctctgac tgctttgccg
     8761 attatcgaaa cccaagcggg cgacgtatct gctttcgttc cgactaacgt catttcgatt
     8821 accgacggtc agattttctt ggaaaccgac ctcttcaacg ccggtatccg tcctgcaatc
     8881 aatgccggta tttccgtatc ccgcgtaggc ggtgctgcac aaaccaaagt gattaaaaag
     8941 ctgggtggcg gtatccgttt ggcgttggcg caatatcgtg aattggcggc gttctcgcaa
     9001 tttgcatccg atttggatga agctacgcgc aaacaattgg agcatggcga agtcgtaacc
     9061 gaactgatga aacagaaaca gttcagtacg ttgaatacgg ctgaaatggc tttgaccctt
     9121 tgggcaatca acaacggttc gtattctgat gttccggttg ccaaagctct ggcttttgaa
     9181 tctgaatttt tgagctttgt ccgtacccaa catccggaag ttttggaagc cgtcaatgct
     9241 tcaggtgcaa tgtccgacga gagcgagaaa acgcttgaag cagccatgaa atccttcaaa
     9301 tcttcttacg cctatcaggc ataaggcttg aagtgaaagg agtctgaaat ggcagtagga
     9361 aaagagattc tcaccaaaat ccgcagtgtt cagaataccc aaaagatcac taaagcgatg
     9421 cagatggtgt caacctctaa aatgcggaag actcaggaac ggatgcgttt ggcgcgtccg
     9481 tatgccgaaa aagtgcgtat ggtgatgagc catcttgcgc aaaccaatac cgatcatggt
     9541 attccgttac tggaatctca tcgggaaatc agacgtgtcg gttttatttt gattacgtct
     9601 gataagggtt tgtgtggcgg tttgaacgcc aacgtgctga aaaagttttt ggcacaagtt
     9661 caagagtatc ggaatcaagg tattgaagag gaaatcgtat gccttggcag taaaggtctg
     9721 atggcgtgtc agagcattgg tctgaatgtg gttgccagtg ccgtaaattt gggcgatacc
     9781 ccaaaaatgg aaatgctgct cggacctttg acagaactct tccaacggta tgagaaacat
     9841 gaaattgaca gaatccatct ggtgtattcg ggttttgtca ataccatgcg tcaagaaccg
     9901 agaatggaag tattgctgcc tatcggtgag aacgtgattg gcgattcagc tcccaaatca
     9961 ccgttcagct gggaataccg ctacgaaccg actgcacttg cagtgttgga atatctggtt
    10021 cgccgctatt tagagtctgt ggtttatcag gcgttgagcg acaatatggc atccgaacag
    10081 gcagcccgca tggttgccat gaaagctgca acagacaatg caggcaatgc catcaaagag
    10141 ttgcgtttgg tatataacaa atcgcgtcaa gctgcgatta ccacggaatt gtcagaaatt
    10201 gtagcaggtg cggcggctgt ctgatgccgt ctgaatcctg tacggaaatt aggatacgat
    10261 aatgagccaa ggcaaaatcg tacaaattat cggtgcggtt gttgacgtgg aatttccacg
    10321 cgacatgatt ccgcgcgttt acgacgcttt gaaattagac gaaaacggtc tgactttgga
    10381 agtccaacag cttttgggcg acggcgtagt ccgtgccatt gcgatgggca gctcggacgg
    10441 tttgaaacgc ggcatgactg tgagcaatac cggtgcgccc attactgtgc cggtaggtaa
    10501 aggtacgttg ggacgcattg tcgatgtatt gggaacgcct gttgacgaag caggtccgat
    10561 cgataccgac aagagccgtg ccatccacca agctgcccct aagtttgacg aactgtcttc
    10621 cacaaccgaa ttgctcgaaa cgggcattaa agtgattgac ttgctgtgtc cgtttgccaa
    10681 aggcggtaaa gtaggtctgt tcggcggtgc cggtgtgggt aaaaccgtga acatgatgga
    10741 attgatcaac aacatcgcca aagcgcacag cggcttgtcc gtgttcgcag gcgtgggtga
    10801 gcgtacccgc gaaggtaacg acttctacca cgagatgaaa gattccaacg tattggataa
    10861 agtggcaatg gtttacggtc agatgaacga acctccgggc aaccgtttgc gcgtcgcatt
    10921 gaccggtttg accatggcgg aatacttccg tgacgaaaaa gacgaaaacg gtaaaggccg
    10981 cgacgtattg ttcttcgtgg acaacatcta ccgttacacc ctggctggta ccgaagtatc
    11041 cgcattgttg ggtcgtatgc cgtctgcagt gggctaccaa ccgacattgg cagaagaaat
    11101 gggtcgtttg caggagcgta ttacctctac ccaaaccggt tctattactt ccatccaagc
    11161 cgtatatgta cctgcggacg acttgaccga cccgtctccg gcaacaacgt tcgcccactt
    11221 ggacgcaacc gtcgtattga gccgcgatat tgcctctttg ggtatttacc cggcagtcga
    11281 tccgcttgat tctacttcgc gccaactgga tccgatggta ttgggtcaag agcactacga
    11341 cgtagcgcgc ggcgtacagt ccaccctgca gaaatacaaa gaattgcgcg acatcatcgc
    11401 catcttgggt atggacgaat tgtctgacga agacaaactg accgtaatgc gtgcgcgtaa
    11461 aatccaacgc ttcctgtccc aaccgttcca cgttgccgaa gtgtttacag gttctccggg
    11521 caaatatgtc gccctgcgcg ataccattgc cggcttcaaa gccatcttga acggcgaata
    11581 cgaccatctg cccgaacagg cattctacat ggtcggcagc atcgaagaag cggttgagaa
    11641 agcgaaaacc ttaaactgag gaggtcggca tgagcatcat gcaagttgag gtggtcagta
    11701 gcgagcaaaa catctattcg ggcgaagcca gctttgtagt ggttccgacc gttcaaggtg
    11761 agcttggtat ttacccgcga cacgagccga ttatgagttt ggtgcggccg ggggctttgc
    11821 gtttgaccgt tccgggcgag gataaagagg ttttggttgc tgtttccggc ggtgttttgg
    11881 aagtacagcc tgataaagta actatcttgg cggatgttgc cgtccgcagt gcggagatgg
    11941 atcaggcacg tgcggaagag gcgaaaaaag ccgcagaagc gggcatttcc caagctaaag
    12001 acgataaggc tttggcggag gcgcataaag cattggctgc tgcgattgca cagctcaaaa
    12061 ctttggacta tatccgttcg cacaagaaat aaacttgttt ggtttggaaa agcacgaccg
    12121 tttggtcgtg ctttttattt tccggattta aattaaagac tgtgtttgga tatggcgttg
    12181 gcaggcatag cctgctttaa ggtattcaat agggcagcct gccgcctaac cgaaa
//
LOCUS       DAXHPG010000045        12101 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXHPG010000045 DAXHPG010000000
VERSION     DAXHPG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.045"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(170..1099)
                     /locus_tag="ACFM6F_000947"
     CDS             complement(170..1099)
                     /locus_tag="ACFM6F_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6164387.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHDLLPQVTDAERDLARIIEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNDIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1230..1979
                     /locus_tag="ACFM6F_000948"
     CDS             1230..1979
                     /locus_tag="ACFM6F_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC6164388.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            2102..2683
                     /gene="petA"
                     /locus_tag="ACFM6F_000949"
     CDS             2102..2683
                     /gene="petA"
                     /locus_tag="ACFM6F_000949"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC6164389.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2702..4051
                     /locus_tag="ACFM6F_000950"
     CDS             2702..4051
                     /locus_tag="ACFM6F_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC6164390.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            4066..4854
                     /locus_tag="ACFM6F_000951"
     CDS             4066..4854
                     /locus_tag="ACFM6F_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC6164391.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            5193..6977
                     /gene="rmuC"
                     /locus_tag="ACFM6F_000952"
     CDS             5193..6977
                     /gene="rmuC"
                     /locus_tag="ACFM6F_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC6164392.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQEQKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHIDLQEKAQGLAVENERLTTQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVRQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAAAVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYAAEDEAV"
     gene            complement(7184..7816)
                     /locus_tag="ACFM6F_000953"
     CDS             complement(7184..7816)
                     /locus_tag="ACFM6F_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC6164393.1"
                     /translation="MTLRYEILSVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNHWLEEGETLKVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7907..8731
                     /locus_tag="ACFM6F_000954"
     CDS             7907..8731
                     /locus_tag="ACFM6F_000954"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC6164394.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKDQEIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQTKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            8800..9363
                     /locus_tag="ACFM6F_000955"
     CDS             8800..9363
                     /locus_tag="ACFM6F_000955"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC6164395.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            9445..9882
                     /locus_tag="ACFM6F_000956"
     CDS             9445..9882
                     /locus_tag="ACFM6F_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC6164396.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            9951..10220
                     /locus_tag="ACFM6F_000957"
     CDS             9951..10220
                     /locus_tag="ACFM6F_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC6164397.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            10220..11995
                     /gene="ptsP"
                     /locus_tag="ACFM6F_000958"
     CDS             10220..11995
                     /gene="ptsP"
                     /locus_tag="ACFM6F_000958"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC6164398.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIAKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
BASE COUNT         2960 a         3031 c         3394 g         2716 t
ORIGIN      
        1 ttgggcaaaa atcaaaaaca gtatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
       61 cgcagacagt acaaatagta cggcaaggcg aggcaacgct gtactggttt ttgttaatcc
      121 actatacatg aaaaaataga aaaactgccg ggtatggttg aataaggggt cagaccggtt
      181 ccaattcgct cagtccgggc aaatctgcaa aaccgcgttc gcgtatgatt tggcaaaaat
      241 tgttcagata gctcttgtcc gtatcttcgg tacggatggc ggcatacagt ttgctttgca
      301 atccgtcggc agtaatttgg cggtggacga catagccttt ttcaaggtag ggcatgactg
      361 tccaataggg aagggcggca atgccacgtc tgctggcaac cagttggata atggcgatgg
      421 tcagttcgct gtgtcggcgc ggcgggttga tgtttttcgg aatcaggatt tttttgggca
      481 aatccagcat ctcgtcggga acgggataag taatcagggt ttccccgata aagtcttccg
      541 ccgtccaaac gtttttggcg gcaagcggat ggtctggtgc gcaaatgccg accatttcgt
      601 aggcaaacag cggttggaaa gaaatatcgt tttgtttttc cgcttcggaa acaatggcaa
      661 ggtcggcacg gtgttgcagc agcagtccga cgggatccgc ttggaatccc gatacgatat
      721 ccaattcgac ttggggccac atcgggcgga attcgcccat ggcgggcatc agccagtcga
      781 aacaggtatg gcattcgacg gcaatccgca attctcctgc ctctccttct ataatgcgtg
      841 ccaaatcacg ttctgcatcg gtaacttgag gcaacaggtc gtgagcgagg cgtaacagcc
      901 tttctccaac cggggtaaag cgcaagggcg tggattttcg ttcgaacaac ggtgcgccgt
      961 agtggttttc aagcatacgg atttggtggg aaagtgcaga ttgggtaagg aaaacccgtt
     1021 tggcggcaag ggagacgctg ccggtttctt caagtgccag cagggtcttg aggtggcgca
     1081 gttcgatgat ggaatccatg aggtttcaga cggcatattg aaccggcgca tattaaaata
     1141 attcatatgc gggtgcaaat cggaaaaatg tgaattccag acattttgcg attagaatgc
     1201 ccgcttgttt aaagcgatta ggaaaaaaga tggtattgcg cagggatttt ctgacttggt
     1261 gtaatgaaac attgcagaca gcgttgttta aagattacgc ccctaacggt ttgcaggttg
     1321 aagggaggga atatatcggg aaaatcgtta cgtcggtaac ggcaagcagg gcagcgattg
     1381 attttgctgt ggagcagaag gcagatttgc ttttggtaca tcacggtatg ttctggaaaa
     1441 acgagttgcc gaccgttact ggttggaaaa aagaacggat tgccgcactg ttacggcacg
     1501 acatcaatat ggcaggctac catctgcccc tggatgcaca tcccacactg ggcaacaatg
     1561 cccaactcgc cgacagattg ggttttgcga cagaaaaacg gttcggcgaa caaaacctgc
     1621 tcaactcggg cagcctgaaa caagccaaga cactcggcgc attggcggcg catattgaaa
     1681 cagttttgca acgtaagcct gtcgtcatcg gcaaacccga acgcgaaatc cgacgggttg
     1741 catggtgcag cggcggggcg caggggtttt ttcagacggc aatagacgaa ggtgtcgatc
     1801 tgtatttgac gggggaaatc tctgaagctc aataccacct tgccaatgaa acgggtacgg
     1861 ctttcatttc ggcagggcat cacgcgacgg aacgttacgg cgtacgcgcg ctggcagaat
     1921 cggcggcaga ggttttcggg ttggaagtgt gccattttga cgaaaacaat ccggcttgag
     1981 tcttgagaaa tatcataaaa ctttacctta ttttaaataa tgctttgagt atcactgcaa
     2041 actgggtaaa attgcaacgt ttaatggctc ggtattccca aaatattagg acgactgaat
     2101 aatggataat caagaaatca acaacggccg ccgccgtttc ctgacactcg cgacctgcgg
     2161 cgcgggcgga gtggcagcat tgggtgtggc aacgccgttt gtggccagtt ttttcccttc
     2221 ggaaaaagcc aaggccgccg gtgctgccgt cgaggtggat gtcagtaaaa tcgaagcggg
     2281 tcagctgctg accgccgagt ggcaaggcaa accgatttgg gtgctcaacc gtacagatca
     2341 gcagcttaaa gacctgaaag gcctgaacgg cgaacttacc gatcccaatt ccgatgcgga
     2401 acagcagccg gagtatgcta aaaacgagac ccgttcgatt aagccgaaca tccttgtcgc
     2461 catcggtatc tgcacccatt tgggctgctc gcccaccttc cgtcccgaca ttgcccccgc
     2521 cgatttgggt gcagactgga aaggcggctt cttctgcccc tgccacggtt cgaaattcga
     2581 cttggccggc cgcgtatata aaggtgttcc tgccccgacc aacctggttg tcccgccata
     2641 taaatacttg agcgacacaa ctatcttggt gggcgaagac taagaataag gaacgataat
     2701 tatggcaaac caaaccaata gcaaagcaaa agcattgtta ggctggatgg acgctcgttt
     2761 ccccctgtct aaaatgtgga atgagcattt ggctcaatac tacgcaccga aaaactttaa
     2821 tttttggtac tacttcggct ctttggccct gcttgtcctc gtgattcaaa tcgtcagcgg
     2881 cattttcctg accatgaact acaaacccga cggcaacctg aacgcctacc atctgcctgc
     2941 tgcctttact gcagtcgagt acatcatgcg cgacgtgtcc ggcggttgga ttatccgcta
     3001 catgcattcc accggcgcat ctttcttctt cattgttgtt tatctgcaca tgttccgtgg
     3061 tttgatttac ggttcgtaca aaaaaccccg tgagttggtg tggattttcg gttctctgat
     3121 tttcttggca ttgatggcgg aagcctttat gggctacctg ctgccttggg gtcagatgtc
     3181 cttttggggg gcgcaggtaa ttattaacct gttctccgcc atccctgtta tcggtccgga
     3241 tttgtccact tggattcgcg gcgacttcaa cgtttccgac gttaccctga accgtttctt
     3301 tgccctgcac gttatcgcgg tacctctggt attgctcggc ttggttgtgg cgcacatcat
     3361 cgccctgcac gaagtgggtt ctaacaaccc tgacggcgtt gaaatcaaga aaaacaaaga
     3421 cgaaaacggt atcccgcgcg acggtatccc attccatcct tactataccg ttaaagacat
     3481 cttgggtgtg gttgtattcc tgattgtctt ctgtgccgta ttgttcttcg caccggaagg
     3541 cggcggctac ttcctcgaag cgccgaactt cgatgcggca aatgcgctga aaacaccccc
     3601 gcacattgcg ccggtatggt atttcacacc gttctacgcc attttgcgtg cgattccctc
     3661 gttcttggga acgcaggttt ggggtgtaat cggtatgggc gcggcggtcg tgttgattgc
     3721 cctgctgcct tggctggata ggggcgaggt gaaatccgtc cgttaccgcg gcccgatttt
     3781 caaaaccgca ttggtcttgt tcatcatttc ctttatcggt ttgggtattt tgggcgcgat
     3841 ggtggcaacc gatacgcgta ctttggtcgc gcgcatcctg tccttcgtct atttcgcatt
     3901 cttcctgggt atgccgttct ataccaagct ggataaaaac aaaccggttc ccgaacgcgt
     3961 aaccatgagc accgccaaac aaaaaatcat gttctttgtt tacgtcggca ttaccgccgt
     4021 gggtgcttac ctgtttgcaa ccaacatctg atgagggcag tcaaaatgaa acaagctatg
     4081 aaaaattggt ttgctgcctt gctgttggca gtacccatgg gtgcggcatt tgcttcgggc
     4141 ggtcatgcac actatgagaa agtcgatatc gatttgcgcg accaagtcag cctgcagcgc
     4201 ggcgcacaaa tctttaccaa ctattgcctg tcctgccact cggcaagcgg tatgcgtttc
     4261 aaccgtttga aagacatcgg tttgactgac gaagaaatca agaaaaacct gatgtttacc
     4321 accgataatg tcggcgatgt catgcattcg gcgatgaacc cgaaagatgc ggcaaaatgg
     4381 tttggtgctg ctccgcccga tttgacattg attgcgcgtt ccaaaggtgc agactacctt
     4441 tacgcttata tgcgcggctt ctataaagat ccgacccgtc cgagcggctg gaacaatact
     4501 gtattcgata aagtcggtat gccccacccg ttgtgggaac agcaaggcgt tcaagccgtt
     4561 gagttggatg ccaaaggtca gccggttatg gtaaaagacg aacacggcga gatgaagcct
     4621 aagctgtatt gggaatctac cggtttgcac agccgccgtc tgcctaacgg caaagtgatc
     4681 caaaaagagt acgacgcata tgtacgcgat ttggtcaatt accttgtgta catgggcgaa
     4741 cctgcacaac tgcaacgcaa acgtataggc tatgtcgtga tgattttcct atttgcggtt
     4801 atgctgcctt tggcgtattt cctgaaaaaa gaatattgga aagacgtaca ctaagcgttt
     4861 ggaacaaaag ggcaaatcct ttagggtttg cccttttttc attttgcctg ccgtttgaaa
     4921 agcctgaatc cgtatgccgt ctgaacatag ctgcaacatt tcagacggca tcattctaaa
     4981 aatgtcagac atcgggaact taatcagggt ttgtggcaga ttgatattga attaggaaaa
     5041 ggtctgaaac cgcaagcagc atggtaaggc gatgtcgaac aggtttaaag ccaatccgct
     5101 atattttttg gagctatgtc gaacaaacag gtagaatcgg aataagcggc ggttttgtcc
     5161 ggtgtgaaaa tgtttgaata ttaggatgat tgatggaact gatgactgtt ttgctgcctt
     5221 tggcggcgtt ggtgtcgggc gtgttgttta catggttgct gatgaagggc aggtttcagg
     5281 gcgagtttgc cgatttgaac gcgcaactgg cggaaaaggc ggcaagatgt gattttgtcg
     5341 aacaggcaca cgcagaaatt gcatcggaat tggccgtttt ggacgggaaa taccgacatt
     5401 tgcaggacga aaattatgct ttgggcaacc gtttttccgc agccgaaaag cagattgccc
     5461 atttgcagga aaaagaggcg gaatcggttc ggctgaagca gtcgtatatc gagttgcagg
     5521 aaaaggcgca gggtttggcg gttgaaaacg aacgtttggc aacccagctc ggacaggagc
     5581 agaaggcgtt tgccgatcaa tatgccttgg aacgccaaat ccgccaaaga atcgaaaccg
     5641 atttggaaga aagccgccaa actgtccgcg acgtgcaaaa cgacctttcc gatgtcggca
     5701 accgttttgc cgcagccgaa aaacagattg cccatttgca ggaaaaagag gcggaagcgg
     5761 agcgtttgag gcagtcgcat atcgatttgc aggaaaaggc acagggtttg gcggttgaaa
     5821 acgaacgttt gacaacgcaa atcgaacagg aacgccttgc ttctgaagag aaactgtcct
     5881 tactgggcga ggcgcgcaag agtttgagcg atcagtttca aaatcttgcc aacacgattt
     5941 tggaagaaaa aagccgccgt tttaccgagc agaaccgcga gcagcttcat caggttttga
     6001 acccgttaaa cgaacgcatc cacggtttcg gcgagttggt caggcaaacc tatgataaag
     6061 aatcgcgcga gcggctgact ttggaaaacg aattgaaacg gcttcaaggg ttgaacgcgc
     6121 agcttcacag cgaggcaaag gctttaacca acgcgctgac cggtacgcag aataaggttc
     6181 agggaaattg gggcgagatg attctggaaa cggttttgga aaattccggc cttcagaaag
     6241 ggcgcgaata tgtggtgcag gcggcggctg tccgcaaaga agaagacggc ggcacgcgcc
     6301 gccttcagcc cgacgttttg gttaacctgc ccgacaacaa gcagattgtg attgattcca
     6361 aggtttcgct gacggcttat gtgcgctata cgcaagcggc ggatgcggat acggcggcgc
     6421 gcgaactggc ggcacacgtt gccagcatac gcgcgcatat gaaaggcttg tcgctgaagg
     6481 attacaccga tttggaaggt gtgaacacat tggatttcgt ctttatgttt atccctgtcg
     6541 aaccggccta cctgctggcg ttgcagaatg acgcgggatt gttccaagag tgtttcgaca
     6601 aacggattat gctggtcggc ccgagtacct tgttggcaac cttgcggacg gtggcgaata
     6661 tttggcgcaa cgaacagcaa aatcaaaacg cgctggcgat tgcggacgaa ggcggcaagc
     6721 tgtatgacaa gtttgtcggt ttcgtacaga cgctcgaaag cgtcggcaaa ggcatcgatc
     6781 aggcgcaaaa cagctttcag acggcattca agcaacttgc cgaagggcgc gggaatctgg
     6841 tcggccgcgc cgagaaactg cgtctgttgg gtgtgaaggc aaacaagcag cttgcacgcg
     6901 atttgaccga acgtgccaat gaaacaacgg cgttgtcgga atctttggaa tatgcggcag
     6961 aagatgaagc agtctgactt gtgcggaaaa atattgtttc agcaggggcg ggaatgccga
     7021 aagcgcggcg taactgtacc ggttctgatt tggggtttgt ttgaagtact taccaaagcc
     7081 ttgtccggcg aggtacaccg gcaaggcggc ggttgtctga gccttttgcg tttcagacgg
     7141 catcagtgca gatgacgccg tctgaagacc gtagtgaagt cggttagaaa aacggattgt
     7201 gccgtttttc gtgtccgatg gaagtcatac gcccgtgtcc ggcgacaact tgcacggttt
     7261 cggggagggc gaataatttg ttgcggatat tattgattaa gtcggcgtgg ttgccgcgcg
     7321 gaaaatcggt tctgcctatg gtttcgtaaa acagcacgtc gcccgcaatc agcagctccg
     7381 cttcggcaca atagaaaacc acgtgtcccg gcgtgtgccc cggaatatgc agcacttgga
     7441 aggcgtagcg tccgactttg agcgtttcgc cttcttcgag ccaatggttc ggcgcaaagg
     7501 cgggcgagac ggggaatccg tattgcgcgg tggtttgcgg cagcgattgg agcaggaatt
     7561 catcgtccgg atgcggcccg aggacaggga ctttatgcgt tttcaacatt tcgaccacgc
     7621 cgcccgcgtg atcgagatgg ccgtgcgtca gccagattgc cgtgagcgta agtttgcggt
     7681 ttgccaacgc ttgcagcagg aacggcacgt cgccgccgac atcggtcagg acggcttcgc
     7741 cgctttcgtc gtcccaaatc agggtgcagt tttggcggaa gggggtaacg gagaggattt
     7801 cgtaacgtag ggtcatggcg tgttcctaaa cggtttttca gacggcatcg ggtttgccgt
     7861 ttgttttatg gcggtttgcc gcctgtttgg atattggggg aatcagatga ttaagaagac
     7921 aatcggcggc atcataccga tttttacggc ggttttcatc cctgcatcag caggcgcggc
     7981 ggatttgatg ctggcgcagg aatacaaaga ccaagagatt gcaggctggg caatgagcga
     8041 gaaactcgac ggcgtgcgcg cctattggga cggaaagcac ctgataagcc gtcagggcta
     8101 cgcgtttacg ccccccaaag gctttaccgc gcagtttccg ccttatcctt tggacggcga
     8161 attgtatagc ggacgcggtc agttcgagca gatttccgct accgtgcgtt ctgtttcttc
     8221 agactggcgc ggcatccgcc tgcacgtttt cgatgtaccc aaggcgcagg gcaatctcta
     8281 ccaacgtttg gcagtcgcaa cgcagtggct gaaaacgcat ccgaacgcgc cgattaccat
     8341 catcccgcaa accaaagtgc gcgaccggca gcacgcgatg gactttttaa aacaaatcga
     8401 agcgcagggc ggcgaaggcg tgatgctgcg tcagcccgaa tcccgttata gcggcggcag
     8461 gagcagccaa ttattgaagc tgaaaagcca atacgacgac gaatgcacgg taacacggca
     8521 ctatgagggc aaagggcgaa acgccggacg gctgggcgcg gtcggctgca aaaaccgaca
     8581 cggcgaattc cgcatcggca gcggtttcaa agacaaagac cgcgacaacc cgcccaaaat
     8641 cggcacactg attacctacc gctaccgtgg ttttacgcgg aaaggcacgc cgaaatttgc
     8701 cacatttgtg cgcgtgcgta ccgaccgctg agcaggacgg ttcagacggc agccgatact
     8761 ttggtttata attttccttt taccgaccga ttccgacata tgaccgattt agaaaccaaa
     8821 cgccttgaaa cacaggcgat gcttgaaaac gccgatcttt tgttcgacca aggccaatgc
     8881 cgtgccgcac tgcaaaaagt ggcggacgag attacgcgcg atttgggcgg caaatatccg
     8941 ctgctgctgc ccgtgatggg cggcgcggtg gtgtttacgg ggcagttgct gccgctgttg
     9001 cgttttccct tagattttga ttatgttcac gtttcccgtt acggcgacaa gctggagggc
     9061 ggcgcgttca actggaagcg tatgcccgat gcggaacaaa tccggggcag gcacgtcgtc
     9121 gtgctggacg atattttgga cgaagggcat acgatgtccg ccattcaagc caaacttttg
     9181 gaaatgggtg cggcaagctg ccgtgcggcg gtgttcgcca acaagctgat cgacaaaccc
     9241 aagcctatcc gagccgatta tgtcggactg gatgtgccga accgttatgt tttcggttac
     9301 ggcatggatg cggcgggctg ctggcgcaat ctgggcgaga tttacgcatt ggacggaaaa
     9361 taaggaagcg atgccgtctg aaggcctttc agacggcatc gcgcccatac gccggtagga
     9421 taatgaagaa caggacgcat caatatgata gggcttttaa tcatcacaca cgaaaccata
     9481 ggcgaagcct accgcaagct ggcacatcat tttttcccgg gcgggctgcc tgaaaacgtc
     9541 cgcatactcg gcgtgcagcc gacggaagac caagacgaca tcatcaacaa cgccattgcc
     9601 gcgcttcagg agtttcccga caacgacggc gtgctgatta tgaccgatat tttcggcgcg
     9661 accccctgca atgccgcccg ccgcctcgtg cgcgaaaaca aatcggcgat tttgaccggg
     9721 ctgaacgcgc cgatgatggt taaggccgtc caatattcgc cggcggcgga agaccttgcc
     9781 gcctttaccg aatgcgtcag ggaggcggcg gtaaaaggca ttttcgccat cacgtccgcg
     9841 cccgaagatt tggtgtgccg gcgcagcggc gatgccgtct gaagaagcgg cagggcagga
     9901 aaacatttta aacggcgtct gctgccgata tacataacac gggaatcgaa atgctcaaac
     9961 aatccatcga aatcatcaac aaactcggac tccacgcccg cgcgtccaac aagttcaccc
    10021 aaaccgcgtc ccaattcaaa agcgaagtct gggttacgaa aaacgacagc cgcgtcaacg
    10081 gcaaaagcat tatggggctg atgatgctcg ccgccgccaa gggtacggtc atcgaactgg
    10141 agacggacgg cgcggacgag gcggaagcga tgcgcgccct gaccgactta atcaacggct
    10201 acttcggcga gggcgaataa tgagtatcgt gctgcacggc gtggcggcgg gcaaaggcat
    10261 tgccgtcggt tgcgcccacc tgattgcgcg cggtacggag gaagtgccgc agtatgatgt
    10321 tgcggaggcg gacaccgatg ccgaagccga acgtttcgat gccgccgtca aagccacgcg
    10381 caaagagttg gaacagctcc gcagcgcaat tcccgaaaac gccccgaccg agttgggcgc
    10441 gttcatctcg ctgcacctga tgctcttgac cgatgttacc ttgtcgcgcg aacccgtcga
    10501 tattttaagg gaacaaaaaa tcaacgccga gtgggcattg aagcagcaga gcgacaaact
    10561 cgccgcccaa ttcgacaata tggacgatgc ctatttgcgc gaacgcaagc aggatatgct
    10621 gcaagtcgtc cgccgcatcc acaacaacct gatcgggcag ggcaacgagt tggaagttgc
    10681 cgacaacctg tttgacgaaa ccgttctgat tgcaaacgac ctttcgcccg ccgacacggt
    10741 tttgtttaaa gagcagcgca ttgccgcctt cgttaccgat gccggcggcc ccaccgggca
    10801 tacggcgatt ttgggcagga gcttggacat cccgtccgtc gtcgggctgc acaacgcgcg
    10861 caaactgatt accgaaggcg aaacggtcat tgtggacggt atcaacggcg tgttgattat
    10921 cgcgccggat gagtcggtgt tgaacgaata ccgccgccgt gcccgcgaat accgcagcca
    10981 caaacgcgat ttgaacaagc tcaaaaaaac cgccgccgcc accgccgacg gggtctgcat
    11041 cgagcttgtg ggcaatatag aatccgccga agacgtgaaa ccgctgcaca acctcggcgc
    11101 agacggcatc gggctgttcc gcagcgagtt tctttacctg aaccgcgata cgatgccgtc
    11161 tgaagacgag cagtacgaag tgtacagcgc gattgtcaaa aaaatgaaag gcaaaagcgt
    11221 aacgatacgg acagtcgatt tgggtgtgga caaaaacccg cgctggttcg ggaaaaacag
    11281 cacgcccaac ggcagcctca accccgcgct gggcatgacc ggcatccgcc tgtgccttgc
    11341 cgaaccggtc atgttccgca cccagatgcg cgccatcctc cgtgcggcgg tacacggccc
    11401 cgtgcggatg atgtggccga tgattacctc cgtatccgaa gtgcgccagt gcctcatcca
    11461 cctcgacacc gcgcaacgcc agcttgccga acgcggcgat gccttcggta aagtcggcat
    11521 cggctgtatg attgaaattc cgtctgccgc gctgaccgtc ggcagtattt tgaaactggt
    11581 cgatttcatc tccgtcggta ccaacgacct gattcaatac atcttgtccg tcgatcgcgg
    11641 cgacgacagc gtcagccacc tctaccagcc cggccatccc gccgtgctga aaatgctgca
    11701 acacgtcatc cgtaccgcca accgcatgga caaagacgta tccgtatgcg gcgagatggc
    11761 gggcgatacc gcgtttaccc gcgttttatt gggtatgggg ctgcgccgtt tttccatgaa
    11821 ccccaacaac atcctgcccg tcaaaaacat cattctgcac agcaatgtcg gacagctcga
    11881 aagcgatatt gcgaaagtca tccgctgcga agacgaagag aagtcggaaa agctgatcaa
    11941 acagatgaac agcgtgtctg tcgaggaaga agccgacttc aaggggcgga aataaatacg
    12001 gcaggtaaaa aatagaaata cttaacaatg cccgcaatct gaaattttgc cattcttgca
    12061 aaatagaaaa ccgaaacaga aacccaaaat cggtcattcc c
//
LOCUS       DAXHPG010000046        12099 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXHPG010000046 DAXHPG010000000
VERSION     DAXHPG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.046"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(454..1599)
                     /gene="dapE"
                     /locus_tag="ACFM6F_000959"
     CDS             complement(454..1599)
                     /gene="dapE"
                     /locus_tag="ACFM6F_000959"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC6164399.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNKYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(1712..2332)
                     /locus_tag="ACFM6F_000960"
     CDS             complement(1712..2332)
                     /locus_tag="ACFM6F_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFC6164400.1"
                     /translation="MLTSKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQVWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTDKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     KLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(2357..2866)
                     /locus_tag="ACFM6F_000961"
     CDS             complement(2357..2866)
                     /locus_tag="ACFM6F_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6164401.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQITRYFNVAAPLHHEDEEEDFFPLLLQYAPQAREGVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDAEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(2961..3512)
                     /gene="azu"
                     /locus_tag="ACFM6F_000962"
     CDS             complement(2961..3512)
                     /gene="azu"
                     /locus_tag="ACFM6F_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC6164402.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(3793..4215)
                     /locus_tag="ACFM6F_000963"
     CDS             complement(3793..4215)
                     /locus_tag="ACFM6F_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC6164403.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDVSIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAAKLEKRHPELAEALANLVRRHGA"
     gene            4347..7097
                     /gene="secA"
                     /locus_tag="ACFM6F_000964"
     CDS             4347..7097
                     /gene="secA"
                     /locus_tag="ACFM6F_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC6164404.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            7112..7264
                     /locus_tag="ACFM6F_000965"
     CDS             7112..7264
                     /locus_tag="ACFM6F_000965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164405.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            7242..9014
                     /gene="dnaG"
                     /locus_tag="ACFM6F_000966"
     CDS             7242..9014
                     /gene="dnaG"
                     /locus_tag="ACFM6F_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC6164406.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            9202..11130
                     /gene="rpoD"
                     /locus_tag="ACFM6F_000967"
     CDS             9202..11130
                     /gene="rpoD"
                     /locus_tag="ACFM6F_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC6164407.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            11298..12099
                     /locus_tag="ACFM6F_000968"
                     /pseudo
     CDS             11298..12099
                     /locus_tag="ACFM6F_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250515.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         3190 a         3386 c         2952 g         2571 t
ORIGIN      
        1 cctaaccctc tcccacgaga gagggaatgg attgccgttg aaataaatcg ctctacataa
       61 aaaatcaatg tgttatctca aacccacgtt aggtctaata aaatggtcgg acatccgtat
      121 ccgacagccc atcttctttt cagacggcat tgcaaattta agtttgacgt gcgttcaaaa
      181 taaggcagtt aatgcgaagc gaaattccgt cggcgtacct gcaacttgac tccctcccct
      241 gtgggggagg gttggggaga gggtaaaacg gggcagatac agacaatatt tccgttaccg
      301 ccccgatgcc ctctccctaa ccctctccca cgggagaggg aatggattgc cgttgaaata
      361 aatcgctcta cataaaaaat caatgtgtta tctcaaaccc acgttaggtc taagcaaatg
      421 gtcggacatc cgtatccggc aagcaagctg ctttcagacg gcatttccag ccaacaaccg
      481 cgccaatatc ccctcataca ccgcagacag cttcggaatg tcgtttagcc gcacgttttc
      541 gttgatttgg tggatggtcg cattggacgg gcctaattcg atgagttctt ttgcaatggc
      601 tttgatgaag cgtccgtccg aagtgccgcc ggtggtggac aattcggtct ctacgccgca
      661 gatttcggca atggcggcgc gtgccacgtc ggtcagtttg cccgcgtggg tcaggaaggg
      721 ctgccccgaa cacgaccact gcaaatcgta ttgcacaccg tgtttgtcca aaatggcgtg
      781 gacgcgttgt ttcagccctg cttcggtgga ctcggtggag aagcggaaat tgaatttgac
      841 gctcagctcg cccggaatga cattggtcgc gcctgtaccg ccgttgatat tggaaatttg
      901 aaagctggtc ggcgggaagt atttgttgcc ttcgtcccag acttcctgcg tcagctctaa
      961 caaggccggg gcaaaagtat gcacgggatt gattgccaaa tgcggatagg caatatggcc
     1021 ttgcttgcct ttgacggtca ggttgcccga cagcgagccg cgccggccgt ttttaatcat
     1081 atcgcccaat ttgtccacgg ctgtcggttc gccgacgatg cagtagtcga taagctcgtc
     1141 gcgcgctttc aatacatcga cgactttggt cgtgccgtcc aacgcgtcgc cctcttcgtc
     1201 ggaagtaatc agaagcgcaa tgctgccttg gtggtcggga tgttcggcaa cgaagcgttc
     1261 acaggcggta acgaagcagg cgatgctggt tttcatgtct gccgcgccgc gcccgtataa
     1321 tcttccgtcg cgctcggccg gttcgaacgg gggcgaatcc catttttcga caggacctgt
     1381 cggtacaacg tcggtatgcc ctgcaaaaca gacgacggga gctttcgtgc cgcgtcgcaa
     1441 ccagatgttt ttggtgtcgc cgaaatggag ttcttcagcc gcaaaaccga ttttgtgcag
     1501 gcgttcggca aggagttttt ggcaatccct gtcgtcaggg gtaacggatg gtcgggaaat
     1561 cagctctttg gcaagctcta gggattgagt ttcggtcata tttgttcact tttgaaatta
     1621 gaccgtctga aacgttctga atgtgatttt cagacggcat ttaggttagg ttggcatacg
     1681 gggtgggtat tttacccatc agtcttctga atcatttgcc gtggcaggct tcgtaaagcg
     1741 gcagcaaatc ttccaccgtt tccgctatcc atttcgcgac atcctgcttg cccaaatcgt
     1801 cgcgctcgat gtgtttgccg atgcagaaaa agtcttcgtc gttttgcaac tttcggtcgg
     1861 attcgctttg ttgtgcgacg gtgcggtagt cgtcatattc gctttccgcg ccgtgccaca
     1921 tatcgaatgc ggcgtatttg tcggtatcga aatcatccaa ccagcggttg tattcgggca
     1981 gcgcaatcgg cgacacgtcg gctttgtagc agtgccaatc caagctgacg ctcaggcggc
     2041 ggcggttcaa taaaatcgat aaaatcgcgg cggaattttt atattgttcg tatttgaagt
     2101 aggcaaagaa atgggcgcgc acctgccagc cgttacacca gcgttcgata tgcggcggcg
     2161 cgaacggcgc acccaattcg gcggcaacct gctggatcag ctgctgccat acctgccagt
     2221 tttctttata gtctgccttg atttgcggaa tgctttcagg ctggtatttt ttaagctggg
     2281 aaaattggaa aaacgggata ttgaacaaat cgcaactttt cgaggtaagc atagtgtatt
     2341 ccttgagacg attaccttat ttgcgccgac gcgccgccat gatttcaccg atttcggtca
     2401 gtttttcttt agggataaag gtgttgccca tatcaaacag cggctcttca atcgccaaat
     2461 gaacatcata tcccgccaca aaacgtttga acgcttccgc atcgggaaca taagcattgt
     2521 ctgcttcgag cttggcaaat tcggcggaaa cagccgccca gttgtcgtgc agcccgatat
     2581 gttggcgtaa aagctcgtcc acaccttcac gtgcttgcgg cgcgtattgc agcagcagcg
     2641 ggaaaaagtc ttcttcttcg tcctcatgat gcagcggcgc ggcaacgttg aaataccggg
     2701 tgatttggcg gatggtttgc aaaacaattt gattgcagcc gttttcggcg atgtagtccg
     2761 acagcatggc gacttgtccg caaaaacggc gcactttgcc gtggcaggca tacagcattt
     2821 caatcggttc ggcaaaggta acgcttttgg tttcaaacgg attcatgttt tcgttctcaa
     2881 cgggcacttt tcaagcagtc attttataat aaaacagcct gcacaaagca ggctgtccgt
     2941 cttttgagac tttaagcgga ttaatcgacc aaagtcactt tgccgttcat caaagcaccg
     3001 tgacctggga aggtacaagc gaatttatat tcgccgtcgg ccaatttagc aggatccaga
     3061 gtcagggaag cttcttcgcc gccgccgatc agtttggtat gggcaacaac gcgtgcatca
     3121 tcaggtttga catagtcggt atcggcagca cctacgccgt ctttaaatac gccgtccatg
     3181 tcttcagctt tggcaatcac gagattgtga cccatgctgg ctttgggttg cgtaccggta
     3241 tgtttcagag tgatggtaaa ctctttacat gctttgctga cttggatgtc tttggtgttg
     3301 aactgcatat tgtcgttgga ttcgacagtt gccgcacagt tgccggcagc aggggcttcg
     3361 gcagcatctg caggagcagc ttcggcggca ggcgcttcgg aagcgggtgc ttcagcagca
     3421 ggagttgcct cggcagcagg cgcggcaggt tcttgagagc aggcagccaa accgataacg
     3481 gcggcagaaa tcagagccag atacgctttc ataacaaatc tccaatcgat aaaataatat
     3541 tcggttttac ggaaatcaaa gtgcaaccgc cattaacaaa accttgaaaa agattccgcc
     3601 gcgttgcaca aacagatgtt tcggagcggc attttgctac aaatttcatt tgaaatcaaa
     3661 gcctgtctgc aagtttacaa tcgtttactt taaaaggggc aattttaccc cgaacctatt
     3721 tctttagtat tagacccatt atcctttact tcttaatatt aacggatgtt tacacaaatt
     3781 cccgtataca ttttatgcgc catgccttct aaccaagttt gccaatgcct ccgccaattc
     3841 gggatgccgt ttttccaact ttgccgccgc cgaaccgaaa ctctccagcg cagccttact
     3901 caaatgcagg gtattggttt tcggcggttt ttccggtttc gggaccagcc tgaccgaaac
     3961 agagcgtatc gaaacatcaa gccctgccaa ctgcggcaat accgacggtg caatcatttt
     4021 caaacgcgat gccgccatat tgtttgccgc caaaaggaca agcctgccgt cttcgataca
     4081 tgccgtctga aaatgcgggt gcaggttggc aggcagcagt tttttcacgg cggcatccaa
     4141 ccgccgccac tgtcccgcct gtttcaaaag tccggaaagc agcgcgtccc gcctgcccaa
     4201 ctgttccaaa ttcataaaac atacacccaa aaagattgaa ataccgcaaa cgcgccttta
     4261 tttcagacgg cattagcact ttgcacaaac gcttgtgtta aaatcgcgtt ttcgccccac
     4321 tattatatca ggcgcaggaa ttattcatgc tgacaaacat tgccaagaaa atcttcggca
     4381 gccgcaacga ccgcttgctg aaacaatacc gtaaatccgt tgccagaatc aacgcgctcg
     4441 aagaacagat gcaagcccta agcgatgctg atctgcaagc caaaactgcc gaattcaaac
     4501 aacgcctcgc cgacggtcag actttggacg gcattttgcc cgaagccttc gccgtctgcc
     4561 gcgaagcgtc ccgccgcacc ctcggtatgc gccacttcga cgtgcagctt atcggcggta
     4621 tggtgctgca cgacggcaaa atcgccgaaa tgcgtaccgg cgaaggcaaa accctggtcg
     4681 ccaccctcgc cgtctatctc aacgcgctgg ccggcaaagg cgtacacgtc gttaccgtca
     4741 acgactacct cgcctcacgc gatgcgggca ttatggagcc gctctacaat ttcctcggcc
     4801 ttaccgtggg cgtgattatt tcagatatgc agccgttcga ccgtcaaaac gcctatgccg
     4861 ccgatatcac ctacggcacc aataatgaat tcggcttcga ctacctgcgc gacaatatgg
     4921 ttaccgacca atacgacaaa gtgcagcgcg aattgaattt tgccgttgtc gatgaagtgg
     4981 attccatctt gattgacgaa gcgcgcactc cgctgattat ctccggtcag gcggatgaca
     5041 acatccagtt gtaccaaatc atgaacaccg ttccgcccca cctcgtccgt caagagacag
     5101 aagaaggcga aggcgactat tgggtcgacg aaaaggcaca tcaggtcatc ctgagcgaag
     5161 caggtcacga acacgccgag caaatcctga cccaaatggg attgctggca gaaaacgact
     5221 ccctctattc cgccgccaat atcgccctga tgcaccacct tatggcggca ttgcgcgcgc
     5281 attccctctt ccacaaagac caacattacg tcatccaaga cggcgaaatc gtcatcgtgg
     5341 acgaattcac cggccggctg atgtccggcc gccgctggtc ggagggtctg catcaagccg
     5401 tcgaagccaa agaaggcgtg gaaatcaaac gcgaaaacca aacccttgcc tccatcacct
     5461 tccaaaacta tttccgcctg tacaccaagc tctccggcat gaccggcaca gccgataccg
     5521 aagctttcga gttccaaagc atctacaacc tcgaaaccgt catcatcccg accaaccgcc
     5581 ctgtacagcg caaagacttc aacgaccaga ttttccgttc cgccgaagaa aaattcgaag
     5641 ccgtcgtcaa agacatcgaa gaatgccaca aacgcgggca gcccgtcctc gtcggcacca
     5701 ccagcatcga aaactccgaa ctggtctccc accttctgca aaaagccgga ctgccgcaca
     5761 acgtcctcaa cgccaaagaa cacgaacgcg aagccctgat tgtcgcccaa gccggcaaag
     5821 tcggcgcgat taccgttgcc accaatatgg cgggacgcgg tacggacatc gttttaggcg
     5881 gcaacctgaa gcaccaaacc gatgccatcc gtgccgacga aaccttgagc gacgaagaga
     5941 aacaggcaca aatcgccgca ctcgaaaacg gctggcaggc agaacacgat aaagtaatgg
     6001 aagcaggcgg cctgcacatc atcggtacgg aacgccacga aagccgccgt atcgacaacc
     6061 aattgcgcgg acgttccggc cgtcagggcg accccggatc cagccgtttc tacctttcgt
     6121 ttgaagaccc gctgctgcgc ttattcgcac tcgaccgcgc cgccgccatc ctcaaccgtc
     6181 tcgcccccga acgcggcgtt gccatcgaac acaacctgct gacgcgccaa atcgaagggg
     6241 cgcaacgcaa agtcgaaggc agaaacttcg atatgcgcaa acaggttttg gaatacgacg
     6301 acgttgccaa cgaacagcgc aaagtcattt acagccagcg caacgaaatt ctgaccagca
     6361 aagacatcag cgacctgatg caggaaatcc gttctgatgt cgtcagcgac ctcgtggata
     6421 cctatatgcc tcccgacagc atggaagaac aatgggacat cccgactttg gagaaccgtc
     6481 tggctgccga attcagactg cacgaagaca tccaatcctg gctgaaggcg gacaatgcga
     6541 ttgacggtca agacatcaaa gaacgcctga tcgaacgcat cgaaaacgaa tatgccgcca
     6601 aaaccgaact ggtcggtaag caggcaatgg ccgatttcga gcgcaacgtg atgttgcagg
     6661 tcatcgacaa ccaatggcgc gaacacctcg ccgctatgga ctacctgcga caaggcatac
     6721 acctgcgcag ctatgcccaa aaaaatccga agcaggaata taaacgtgaa gcctttacca
     6781 tgttccaaga cctgtggaac ggcatcaaat tccatattgc ctccctgctt acctcggttc
     6841 aaatcgaaca aaaccctgtc gcggtggttg aagagcaacc catcggcaac atccagtcca
     6901 tccattccga atcgcccgat atggaagaac ttttgggtca gtcgcaaacc gatctggtta
     6961 ccgaagcctt taatcccgat gggacagatt tcagccccga agccttggaa gcgcgggggc
     7021 aaatcgtcca ccgcaacgac ccctgcccct gcggcagcgg tttgaaatac aaacaatgcc
     7081 acggcaaact ggcttaagcg tttgaacgca aatgccgtct gaacatcccg ctcccgtttc
     7141 agacggcatt ttgcctgaac cgccacatcc gactgccatt ccgaaaaatc ccgatttcgt
     7201 accgtccgta ccaaaaacag acatcccgtc cgccccacat catgattcca tccgacttca
     7261 ttgacgagct tttagccaaa accgatattg tcgatattat cgacgagcag gttccgctga
     7321 aaaaaggcgg ggcgaactat atggcgtgtt gcccgttcca caaggaaaaa acgccgtcgt
     7381 tttcggtcag tccaaccaag cagttttacc attgtttcag ttgcggggcg catggttcgg
     7441 cgattggttt tgtgatggag catcaggggc tgtcgtttcc ggaggctgtg cagtttcttg
     7501 ccgaccgtgt gggcatggtt gtgcctaaag tgcgtgggca gaacgataat cccgaagtcc
     7561 gtgccgaacg taagaaaaaa cagcagacgc tggaagaaac gacggcggcg gcggctgatt
     7621 tttacgcgca acagctaaaa ttcaatccgg cggcaaaagc ttatttggac aagcgcggct
     7681 tgagtgcaga agttatcgcg cattatggtt tgggctacgc gcccgacggc tggcagcctt
     7741 tggcgcaagt gttccaaccg tatccgaata ccgcgttggt ggatacgggg atggtgattg
     7801 acaatgaggg gcggtattac gaccgcttcc gccatcggat tatgttccct atccgcaatc
     7861 cgcgcgggca ggtcatcggt ttcggcggca gggtgctgga cgactccaaa cccaaatatc
     7921 taaactctcc cgatacacct ttgtttgaca aagggaaaaa cctttacggc ttgtatgagg
     7981 ggcgtgccgc tgtcaaggaa gcggagcgga ttctggtggt cgaaggctat atggacgtgg
     8041 tcgcgctggc acagttcggc gtgggctacg gtgtggcggc cttgggcacg gcaacaacag
     8101 ctgaacacgt caaaatccta atgcgtcagg cagacagtat ttatttctgt ttcgacggcg
     8161 acagcgcggg gcgaaaagcg gcttggcgcg cgctggaaaa cgcgttgccg cagttgaaag
     8221 acgacaaatc gctgcatttt ttgttcctgc ccgaggaaca cgaccccgac agttacatcc
     8281 gtgcctacgg caaagcgcaa tttgaagacg cgcttctgaa tcaaagcaag cctttgtcgg
     8341 agtatttctg ggaacacctt tcagacggca ttcatctcaa tacgcaggaa ggcaaggcgg
     8401 aattggtgaa aaccagttcg ccgcttttgg cgcagattac cgcgccggca ttggcttatt
     8461 tgttaaaaca acggcttagc gaactggtcg gcatcgaccc cgacaacctc gcgcaactgc
     8521 taggacagga agcgccgaag cggcacgtca aacaaaaaaa ctacaaactg cctccgattt
     8581 ccgtcaaaca gcccgtcatg ctgacgctgg tacagcggca aatccgcagc ctcttgataa
     8641 atccggattg ggctgcatat atagacctgc ccgattatct ggcgttggac ggtgatttcg
     8701 cctgccttgc caatcttgcc gaatcgatta aaaaccatgc cgccgtaccc gaaaccgctc
     8761 aggttttaga gtatatgcgc ggctcgcctt acgaagaaac gataacccga atcttccatt
     8821 caacgcacca atcggaagaa atgaacagca gcagtgaaga agattgcgag aatttccaaa
     8881 tcggcatgaa aaaactgctc aatgagttaa aatacagcca aatcgaagca ttgaaacaaa
     8941 aaagcctgca atccggctta aatgaaagcg agaaaaaact tttgctgtcg ttgctgaccg
     9001 caaaacaaaa ttgaccggcg gattccgcca cccgtaaacc cgttatgccg tctgaaaagc
     9061 acttaccccg gctgcaacaa cgacacctgc agaacaccca tccccaaaag ccttcagacg
     9121 gcatcagagt accctactct gccacgcctt caggtgcgtc caaacgcaaa ccgtcggcat
     9181 cttgccaaca gaaagcagac aatgtccaaa aaccaaaatc acgaagaata ccaagacgat
     9241 gcccgacccc tgacgattga ggaacaacgc gcgcgcctgc gccaactcat cattatgggc
     9301 aaagaacgcg gctacatcac ctactccgaa atcaatgacg cactgcccga cgatatgtcc
     9361 gatgccgatc aaatcgacaa catcgtcagt atgatttccg gtttgggtat ccaagttacc
     9421 gaacacgccc ccgatgcgga agacatattg ttaagcgaca atgccgccgt taccgacgat
     9481 gatgccgtcg aagaagccga ggccgccctt tccagcgccg actccgagtt cggcaggacg
     9541 accgaccccg tccgtatgta tatgcgcgaa atgggacagg tcgacctgct gacccgcgaa
     9601 gacgaaatca tcatcgccaa aaaaatcgaa aacgccctga aaaatatggt tcaggccatc
     9661 tccgcctgcc cgggatccat tgcggaaatc ttagaactca tcgaaaaaat ccgcaaagac
     9721 gaaatccgcg tcgacgaagt cgtagaagcc attatcgacc cgaatgaagt attgctcaac
     9781 gaattgggct tggggcactt ggaaaccaca gcgcccgaga aaccttccaa cgacaattcg
     9841 gacgaaaacg aagacgacga agaatcggaa gaagatgcgg atgaaatctc ggcagccaat
     9901 ctcgccgaat tgaaacaaaa agtcatcggc cactttgccc aaatcgaaaa agactacaaa
     9961 aaaatgatcg gtcgtttgga aaaacaccac agccggcaca aagactatct cgcctaccgc
    10021 gacgcgattg ccaacaaact gctggaagtc cgtttcgcca cccggcaaat cgacagcctc
    10081 agcagcagcc tgcgcggaaa agtagaaaac atccgcaaac tcgaacgcga aatccgcgac
    10141 atctgcctcg accgcgtcca tatggagcgc gactacttca tccaaaactt cctgcccgaa
    10201 atcaccaatc tggaatggat tgaagaagaa atcgccaaag gcagggtttg gagcgacgcg
    10261 ctcgaccgct tccgccacgc catcctcgaa aaacaaaccg agttggcaga tatggaaaaa
    10321 gaaacccgca tttccatcga agagttgaaa gaaatcaaca aaaatatggt gtcgagcgaa
    10381 aaagaaaccg cagccgccaa acaggaaatg attcaggcaa acttgcgcct cgtcatttcc
    10441 attgccaaaa aatacaccaa ccgtggcttg cagttccttg atttgattca ggaaggtaac
    10501 atcggcctga tgaaagcggt cgacaagttc gaataccgcc ggggctacaa attctccacc
    10561 tacgcaacct ggtggatccg ccaggcaatc acccgttcga ttgccgatca ggcgcgtacc
    10621 atccgcattc cggtacatat gattgaaacc atcaacaaga tgaaccgcat ctcgcgccaa
    10681 caccttcaag aaaccggcga agaacccgat tccgccaaac ttgccgaact gatgcagatg
    10741 cccgaagaca aaatccgcaa aatcatgaaa atcgccaaag agccgatttc gatggaaacc
    10801 cccatcggcg acgacgacga ttcgcacttg ggcgacttca tcgaagatgc caacaatgtt
    10861 gcgccggccg atgcggcaat gtacaccagc ctgcacgaag taaccaaaga aatcctcgaa
    10921 agcctgacac cgcgtgaggc aaaagtcctg cgtatgcgtt tcggcatcga tatgaacacc
    10981 gaccacacgc tggaagaagt cggcagacag tttgacgtaa cgcgcgaacg catccgacaa
    11041 atcgaggcaa aagcactccg caagctgcgg catccgacaa gaagcgaccg tttgagaagt
    11101 ttcttggaca gcgaagacag caagctgtaa accaaaaaac cgcaggtttc aaatacctgc
    11161 ggttttttct tacacaataa acaacgcttc cacatatccc acactcctat cccgagacct
    11221 ttgcaaaatc ccccaaaatc ccctaaattc ccaccaagac atttaggagc accttcttcc
    11281 aacaaaccgc ccaagccatg attgccaaac acatcgaccg tttcccgcta ttgaagttgg
    11341 accgggtgat tgattggcag ccgatcgagc agtacctgaa ccgtcaaaaa acccgttacc
    11401 ttagagacca ccgcggccgt cccgcctacc cgctgttgtc catgttcaaa gccgtcctgc
    11461 tcggacaatg gcacagcctc tccgatcccg aactcgaaca cagcctcatc acccgcatcg
    11521 atttcaacct gttttgccgt tttgacgaac tgagcatccc tgattacagc accttatgcc
    11581 gctaccgcaa ttggctggcg caagacgaca ccctgtccga attgctcaaa ctgatcaacc
    11641 gccaactgac cgaaaaaggt ttaaaaatag agaaagcatc cgctgccgtc gttgacgcca
    11701 ccattattca gaccgccggc agcaaacagc gtcaggccat agaagttgac gaataaggac
    11761 aaatcaacgg ccaaaccaca ccgagtaagg acagcgatgc ccgttggatc aagaaaaacg
    11821 gcctctacaa actcggttac aaacaacata cccgtaccga tgcagaaggc tatatcgaga
    11881 aactgcacat tacccccgcc aatgcccatg agtgcaaaca cctgtcgccg ttgttggaag
    11941 ggttacccga aggtacgacc gtctatgccg acaaaggcta cgacagtgcg gaaaaccggc
    12001 aacatctgga agaacatcag ttgttggacg gcattatgcg caaagcctgc cgcaaccgcc
    12061 cgctgtcgga agtgcaaacc aaacgcaacc gatatttgt
//
LOCUS       DAXHPG010000047        12076 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXHPG010000047 DAXHPG010000000
VERSION     DAXHPG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.047"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(332..1108)
                     /gene="lpxA"
                     /locus_tag="ACFM6F_000969"
     CDS             complement(332..1108)
                     /gene="lpxA"
                     /locus_tag="ACFM6F_000969"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC6164408.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVIGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(1195..1644)
                     /gene="fabZ"
                     /locus_tag="ACFM6F_000970"
     CDS             complement(1195..1644)
                     /gene="fabZ"
                     /locus_tag="ACFM6F_000970"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC6164409.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(1678..2724)
                     /gene="lpxD"
                     /locus_tag="ACFM6F_000971"
     CDS             complement(1678..2724)
                     /gene="lpxD"
                     /locus_tag="ACFM6F_000971"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC6164410.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGNDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(2757..3257)
                     /locus_tag="ACFM6F_000972"
     CDS             complement(2757..3257)
                     /locus_tag="ACFM6F_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC6164411.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(3323..5716)
                     /gene="bamA"
                     /locus_tag="ACFM6F_000973"
     CDS             complement(3323..5716)
                     /gene="bamA"
                     /locus_tag="ACFM6F_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC6164412.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMFGGEVGIAGGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(5773..7113)
                     /gene="rseP"
                     /locus_tag="ACFM6F_000974"
     CDS             complement(5773..7113)
                     /gene="rseP"
                     /locus_tag="ACFM6F_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC6164413.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPITSWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(7148..8383)
                     /gene="ispC"
                     /locus_tag="ACFM6F_000975"
     CDS             complement(7148..8383)
                     /gene="ispC"
                     /locus_tag="ACFM6F_000975"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC6164414.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAVQCRTFRPEYAVVADAEHAARLEALLKGDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(8388..9185)
                     /locus_tag="ACFM6F_000976"
     CDS             complement(8388..9185)
                     /locus_tag="ACFM6F_000976"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC6164415.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMSGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAIMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVIGLVWVADVCAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(9188..9934)
                     /locus_tag="ACFM6F_000977"
     CDS             complement(9188..9934)
                     /locus_tag="ACFM6F_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC6164416.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(9990..10547)
                     /gene="frr"
                     /locus_tag="ACFM6F_000978"
     CDS             complement(9990..10547)
                     /gene="frr"
                     /locus_tag="ACFM6F_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC6164417.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(10728..11855)
                     /locus_tag="ACFM6F_000979"
     CDS             complement(10728..11855)
                     /locus_tag="ACFM6F_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC6164418.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(11931..12006)
                     /locus_tag="ACFM6F_000980"
     tRNA            complement(11931..12006)
                     /locus_tag="ACFM6F_000980"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11971..11973),aa:Thr,seq:cgt)
BASE COUNT         2275 a         3083 c         3761 g         2957 t
ORIGIN      
        1 tttaaaacac cacttttgta gtgtccagta atttcagcac agaatattca ataagacaat
       61 atgttctttt gaaaaatact tttggttttt tcgccgaaag caggtcggtt caagctgcgg
      121 aaattgtttg caattcttta aaagcagcgg cggaggtcac aatgaaatgt ccgaatgggg
      181 atgtggtcgg cggcagaaat catcaatgct gccgactgcc atacttctga aatctacaaa
      241 atgatgcatc gatcaaacaa tataccgctt taaaaaaacc gatgccgtct gaaacgcttt
      301 cggggtttca gacggcatca aaagggtacg gtcagcggat gatgccgcgc gccgattgtg
      361 cgaaaaagtc tcggaatacg gcaagctcgg cttgggtttc ggcgcggcgg aggatgtctg
      421 ccttggcctc ttcaaacgga atgccgcgat ggtagagggt tttgtacacg tctttgacgg
      481 cggaaatctg ttctgcggta aaaccgttgc ggcgcatgcc ttcgctgttg agccccgccg
      541 gttcggcgcg gtagcccgat gccataaagt agggcggtac gtctttgtgt acgcctgcgg
      601 cgaaggcggt catggcgtag tcgccgatgt ggcagaattg gaaaaccagc gtgtagccgc
      661 ccaaaacgac gtagtcgccg atggtaacgt gtccggcaag cgaggcgttg ttggcgaaga
      721 tggtgtggtt gccgatgacg cagtcgtgcg cgaggtggca gtacgccata atccagttgt
      781 cgtcgccgat acgggtttcg ccgatgccgg ttaccgtgcc taaattaaag gtggtgaatt
      841 cgcggatggt gttgccgttg ccgataatca gcttggtcgg ctcgtcgcgg tattttttgt
      901 cctgcgggat ttcgccgagg ctggcaaatt ggaaaatgcg gttgttttcg ccgatgctgg
      961 tgtggccgtt gatgacggcg tgcggaccga tttcggtatt cgcgccgatt tggacgttgg
     1021 ggccgataac ggtgtacgcg ccgactttga cgccggagtc gagttcggct ttggggtcga
     1081 tgacggcggt cgggtggatg agggtcatgt ttttcctttc ctgtcgtgtt gccgcgaaga
     1141 tgcgcgacgg caacaggttg tctgaaaact ttcagacgac ctttttctga acactcaaac
     1201 tacgcgtttg gcacacatga tgatggcttc gacggcaact tgcccgtcca ctttggcgac
     1261 ggcgttgaat ttgccgatgc cgcgccgact ggtcagcagc tcgacttcaa agacgagttg
     1321 gtcgccgggg atgacttggc gtttgaaacg ggcttcgtct atgccggcga agaagaagaa
     1381 ttcgttttct ttgcgcccgc cttcgctcaa aatcgccaac gtgccgcacg cctgcgccat
     1441 cgcttcgatg atgagtacgc cgggcatcac gggcaggtcg ggaaaatggc cttggaactg
     1501 gggttcgttt atggtgacgt ttttaatcgc ggtcagggtt ttcatcggct cgaaggcggt
     1561 gatgcggtcg agctggagaa acggatagcg gtgggggatg agtttttgga tgtctttggc
     1621 ttcgatgggg agttgtacgt ccatgtctgt cgtattcctt gaataaagtc ggtttggtta
     1681 tttgctgtct tgaccggcat ctgaaagctg ctgctccagt gttttgagcc gtttgttcat
     1741 ttcgcttaag cggtggatgt aaacagcgtt gcgcgcccat tctttatggg tggacatcgg
     1801 gaagatgccg gcgaggtgtt tgccgctttc ggtaatgctg tgggtgacgg acgtgccgcc
     1861 gccgatggtg gttttgtcgg cgatttcgat gtgtccgacc gtaccgacgc cgccgccgat
     1921 gatgcagtag ctgcctatgg ttacgctgcc tgagatgccg gttttggcgg cgatgacggt
     1981 gtgcgaaccg attttgcagt tgtgtccgat ttggacttgg ttgtcgattt tggtgccgtt
     2041 gccgacggtg gtgtcgctca tcgcgccgcg gtcgatgttg gtgttcgagc cgatttctac
     2101 gtcgttgccc agcgttaccg cgccggtttg cgggatttta aaccatgaat cgccggcaaa
     2161 agcgagtccg aaaccgtccg cgccgatgac cgcgccgctg tggatttcga cgcgtctgcc
     2221 cagtgtgcag ccgtaataaa cgacggcgtt gggatgcagg acgacttcgt cgcccagttt
     2281 gcaatcgtgt tggacgacgg cgtttgccaa gatgcggcag ccttcgccga gcacggtgtt
     2341 tgcgccgatg tagacgttcg cgccgatttc gcagctggcg ggaacggtcg cgcccggttc
     2401 gacgacggcg gtcggatgga tgccgccgcg cgctttgacg acgggtgaaa acaggcgggc
     2461 gactttggcg aaatagagat aggggtcgtc ggcgacaatc aggttgcgcc cttcaaatcc
     2521 gtctgccgct ttggcggaaa cgatgaccgc gcccgcgctg ctgtcgtgga cttcggcttt
     2581 gtatttcgga ttggcaagga agctgatgtg ttccgcctgc gcgtctgcga gcgggcgcac
     2641 ggcggtaacg gaaatgtcct cgccgcgcca ttcgccgccg agccgcgcgg tgatttggga
     2701 cagggtgtag gtggccggaa tcatggtttt cctgttcggt atgccgtctg aaagggtcag
     2761 cgggcgttca tttctttaat gacgctgtcg gtaacgtcgt attgggtgtt gacgtaaatc
     2821 acgttctgca aaatgacatc gtaaccttcc tgtttggcga ttttgacgat gacgcggttg
     2881 gcgttttgct ggagggaggc aaactcttcg ttgcggcgga ggttgtagtc ttcttcaaac
     2941 tgcgcctgtt ttttgcggaa cgctgcgacc agcccgcgcc atttttcttc ggcttgcgcc
     3001 ttttttgcgt ttctgagttt gccttcggca agctgccttt ccaaatccag accttcgcgt
     3061 tgcagttttt gcaattcgtc ctgacgggcg gaaaattcgc tgtccagcgt tttttgaatc
     3121 ttgcgcgcct gcttggattc gaggtagatg cgctcggtgt tgataaagcc gattttttgg
     3181 aaggtgtcgg cgtgcgcgcc tgcggtgcag cacaaaccga tcagagccgc ggcaaacgcg
     3241 cgggtcaaac gggtcatggt aaaactcctt cgaatgttgc cgcgaaatgc cgtctgaagg
     3301 gcttcagacg gcatttgcgg gattagaacg tcgtgccgag ttggaattgg aagcgttgga
     3361 tttcgtcttc cggttttttc ttcagcgggt aggcgtagct gaatttcatc gggcctaaag
     3421 gcgagagcca ggtaaccgcg ccgccggcgg aatagcgcaa ttcgttggta aaggtggatt
     3481 tatgggtatt gccggcgccg taaatgtttt gaaccctgcc gccggtcgcg gaactgctgt
     3541 tgtcgtcgta ggttttgccg tcccacacgc tgcctgcgtc ggcaaacagg ctcaggcgga
     3601 cggtgcgcgc gtctttcgcg ccgggcatcg ggaagagcag ctcggcggag acgttggctt
     3661 ttttgttgcc gccgtagctg attttttcgc cgtattcgtc atacactttc ggaccgagcg
     3721 tgccgctttc gtatccgcgc accgaaccca ggccgccgcc gtagaagttt tcaaagaagg
     3781 ggatttcttt ggttttgccg tagccgcccg caatgccgac ttcgccgccg aacatcagcg
     3841 tgaaggtttt gcttaagggg aagaaccagg tttggttgtg ggtggcggag tagtattgca
     3901 gtttgctgcc gggcagggcg atttcagcgt tcacgcccgt caggtagccg cgcgtcggcc
     3961 ataacgcgct gtcggttttg ttgcgccccc agccgacggt acctttgtac agccagcctt
     4021 tgaagctgcc gtctgtgccg tcggttttgc cgtatttctt gataaagtcg gcatagtgtt
     4081 tgggcgcttt gttgtaggtg ttgacggtca ggtgttctgc caccaaaccg aaattcacgc
     4141 ggtcgtattc ggtaacaggc acgctcatgc ggatgcctgc gcctgccgtg gtggttttat
     4201 attgtttgat gctggtcgat gctttgcgcg ggtcgaaggc ttttccgtaa acatcgtagc
     4261 ctaggctgac cccgtctgcc gtgaagtacg ggtcggtaaa cgacagcgag ccgttgagcg
     4321 tggttttgct tcgtgaggcg cgcagggcgg ccgacttgcc cgtaccgaac aggttgtctt
     4381 gggaaacgcc tgcggacatg accaggccgg tatcctgtac ccagcccgcg ctcaagtcga
     4441 gcgagccggt ggaacgttcg gtcaggctca tgttcaaatc gactttgtcg ggtgtgccgg
     4501 caagcgggac ggcatcaaac tgtacgttgt cgaagtagcc caaaagctcg acgcgctctt
     4561 tggagcgttg cagcttggag gtgtcgtaag gcgcggattc catttggcgc aattcgcggc
     4621 gcacgacttc gtcgcgggtt ttgttgttgc cggtgatgtg gatttcgttg acgtagattt
     4681 tccggcccgg ttcgatgtgc aggacgaaat cgacggtttt ggtttcggcg tttggcagcg
     4741 gctgtacgct gatttcgctg tatgcgtagc ctgccgagcc catgcggttc tgaatctcac
     4801 ccaaaacggc ggtcatctgc tggcgttcgt accatttgcc gggcttcatg gtcagcagtt
     4861 tttccagttc ggctttgggg acttcgttgg tgtcgccttc gatggagact ttgccccaac
     4921 ggaaacgtcc gccttcgtgg acggtgattt tgatggtctg cttggttttg tcttcgttgg
     4981 tttggatgtc ggtatcgagg atgcggaaat cgaagtagcc gttgttctgg tagaagtcgg
     5041 ttactttttc catgtcttgg gcaaatttct gctcgttgaa ttggttgctt cgtgtcagcc
     5101 atgtccaaat gccgccttcg gtcagcgaca tctgccgcat cagtttgcgg tcggaataga
     5161 cttggttgcc ttcaaattcg atgtcggtga ttttggcgga tttgccctcg tcaatcgtga
     5221 tgtcgatgtc gacgcggttg cgggcgagtt tggttacttt gggcgtgatt tggatattga
     5281 gtttgccgcg cccgaggtat tcttctttca ggccggcgac tgcctgattg agtgtcgcct
     5341 gattaaagta ttgcgactgc gccagcccga acgattcgag gtttttctta atggcgtcgt
     5401 tttgcagcat ttttgcgccg gtgatgttga gcgagccgat ggtggggcgt tcgataacgg
     5461 tcagcaggag ctgcccgtcc gcagtttcga cgcgtacgtc gtcaaagaaa ccggtggcgt
     5521 acaggctttt gatgatggca ctgccgtgtg tgtcgttgta ggtgtcgccg actttgacgg
     5581 gcaggtagtt gaatacggta ctcggctcgg tacgctgcaa gccttcgacg cggatgtctt
     5641 ggatggtgaa gtcggcaagt gccaaaggcg atatgcccaa gaccatcagt gcggaagcaa
     5701 tctgtttcag tttcattgtc agttccttgt ggtgcggaat gcggtttcag acggcattcc
     5761 gaaacgtaaa atctaaccga gcagccgggt aacgtcgttg aagaaggcga ccgccatcat
     5821 cagcatcatg agggcaagcc cgaagcgcaa accgatgttt tggacgcgtt cgcccaaagg
     5881 tttgccgcgt atccattcgg cagtataaaa cacgaggtgg ccgccgtcca aaacggggac
     5941 gggcagtagg ttcagcacgc cgaggctgat gctgaccagt gccaaaaatt ccaaataact
     6001 ttgcaagccg agttcggcgg actgtccggc aatgtcggca atggtcagcg gcccggaaat
     6061 atggctgacg gaggcgttgc cgctgattag tttgccgaaa aatttgaggg ttgtccacga
     6121 gtgggaaacg gttttttccc agcccatgcc gaatgcgcgg acaacagacg gacggtagct
     6181 gcggcggatt tgcgcgtccc acgccctgtc cggctgcgga cggaggccga cgcgcccgat
     6241 cagggtgtgg tcgggctgtt cgacagtatc ggggcggatg tcggcggtat gggtttgtcc
     6301 ggcgcgttcg tagttcaggg tgattttttt gccggggctt tggcgggtca ggtttgccca
     6361 ttcttgccat gaggtaatgg gtttgccgtc ggcggcagtc agcctgtcgc ccggtttcag
     6421 gcctgctttt tcggcggggc tgcctttttc cacgccaccg gcaacggttg tgattttaaa
     6481 gggcatcagt ccgatgtagc cttggttttt tgcgatttta ccggcttccg gcgtgcctgc
     6541 ggcatcgatg gtgcggacgg tttgcgcgcc tgatgccgtc tgaacgccga cggcgacttt
     6601 gccggcttcg aggttgagga cgatttcggt ttgcgcgctg ccccaatctg caacgggtgt
     6661 gccgttgacg gattgtattt tgtcgccgct ttggaagccg gcgcgggcgg caatggtgtc
     6721 gggttcgact gtgccgacat aggggcgcag ttcggttacg ccgaaggaaa agctcagtcc
     6781 gtacagcaaa accgccagtg cgagattggt cagcgggccg gcggcgacga tggcgatgcg
     6841 tttggcgggg tgttgtttgt caaaagcgta gggtaaatcg gcttctgata cttcgccttc
     6901 gcgcgtatcg accattttga cgtaaccgcc caacggaatc ggggcgaggc accattcggt
     6961 gtcgccgcgc tttcgggtga aaaacggttt gccgaagccg acggaaaagc gtacgacttt
     7021 gacgccgcac aatctggcaa cgatgtagtg tccgaactcg tgcaggctga ccaaaatcag
     7081 gatggcgaag ataaaagcta gaagggtgtg caaatggttt tcctttgata acggtgttca
     7141 gacggcatca gcgcagtgtg ccgataaatg cccgcgcttg tgcgcgtgtc cgggcatctt
     7201 gcgccaagag cccccctatg tcgcctatgc cgtctgaaaa gtcttgtgaa agacaatggg
     7261 cgacggtttt ggcaatgtcg gtaaacttaa tctgtccgtc caaaaaggcg gcgacggcgg
     7321 cttcgttggc ggcgttcaat acgcagggcg cggctccgcc tgcgttcatg gcttcatagg
     7381 cgagcttcag gcaggggaag cggtcaaagt cgggcttttg gaaggtcagc gcggacaatg
     7441 cgtcgaaatc caggtcgccg acacccgaat cgatgcgctc gggcaaaccc aaacaataag
     7501 caatgggcgt acgcatatcg ggattgccca gttgcgccaa cacggagccg tcgcggtagc
     7561 gcaccatgct gtgtatcacg gattgcggat ggatgacgac ttcgagcttg tcaggcggac
     7621 agttgaacag ccaatgcgct tcaatcagct ccaaaccttt gttcatcatg gtggcggaat
     7681 cgacggagat tttgcgcccc atgctccaat tggggtgttt gaccgcttgg gcgggcgtaa
     7741 tgctgtcgaa cgtgtttaaa tcggcggtca gaaacgggcc gccggaagcg gtcaggataa
     7801 tcgaagcgat gccgtgttcg ttcagacggc cggcgtaatc gcgcggcaaa acttggaaaa
     7861 tggcgttgtg ttcgctgtcg acgggcagca ctgccgcgcc gtttgcacgg gcggtttcca
     7921 taaacaacgc gccggaaacc accagcgtct ctttgttcgc cagataaatg gttttgcctt
     7981 tttgcgccgc tgcgagcgcg gaaggcagcc ccaccgcccc gacgatggcg cacatgacac
     8041 cgctgacttc gtcggcagag gcaacgtcaa ccaatgcctg cgcgccgtgt aaaacctgcg
     8101 tcgccgtgcc gtcgcctttc aacaaagctt caagccgggc ggcgtgttcg gcatcggcaa
     8161 cgacggcata ttcggggcgg aacgtccggc attgcaccgc cagtttctcg acctgcttat
     8221 gccctgccag cgcgaatacg cggaattttt cggggtggcg ggagacaacg tccagcgtgc
     8281 tttcgcctat gctgccggta ctgcctaata tggtcaggac ttgtggtgtc ataatgggga
     8341 taactttata ccggatgccg tctgaagcgt tttcagacgg catagaatca atttaaaacc
     8401 gacatcatcg ccgcatagac gctgataacg gcaatcaggc tgtcggtacg gtcgaacacg
     8461 ccgccgtgtc cgggcagcag gttgctgctg tctttgatgc ctgccgcgcg cttgagccag
     8521 ctttccaaaa ggtcgccgca tacgctgaca acggtcaaca ccaaaccgat taacacggta
     8581 tcgaaccagc ctgtatcgaa tgccagccag ccggcacttc gtacggcggt catgtacacg
     8641 gccacgcaaa ccgcgccgcc gattgcgcct tcccagcttt tgccagggct gattgccggt
     8701 gcgattttgt gtttaccgaa cgccttgccg ctgaaatacg cgcaaacgtc ggcaacccag
     8761 accagaccta tcacggcgag cagcggcagg gcatcatcgg gatgcgggcg cagggatacg
     8821 agcgcgaacc aaaacggcat gaccagaagc caaccgacgg cataaacctg ccaaccgccg
     8881 ttgagcctcc atttgaatct caaccataaa ggcatgatgg cgagccaaaa tgccaaaaca
     8941 acataccaaa ccaaattagg cagcatccag ccgcccgcat aggcaaccac gccgaaaacc
     9001 aaggttgcgg cgaggtaatg gttggtttta attttgcaca aaccgctcat acgggcatat
     9061 tcccacaagg caatcagggc aatcagtccg caaaatgcag cccacaacca ttgcggcgcg
     9121 taaaacagca tgcccagcat cagcggcagc agccacatag cagttatcac ccgttgtttc
     9181 agcatattca gttcctttgc tgttcgatag gcagttgctc ggaggtgcgt ccgaaccgcc
     9241 gttcgcgttt ttggaacgaa gcgacggcat cgtccaaagc cttgccgtca aaatcgggcc
     9301 acaaaatatc ggtgaaatac agttctgcat atgccatctg ccagagcagg aaattgctga
     9361 tgcgcgtttc gccgccggtg cggatgaaca aatccggttc cggtgcatcg cccagcatca
     9421 agtgtttcgc cagcgtgtct tccgtaatct cggatacgcc ttcggcaatc agtttgtttg
     9481 ccgcctgcaa aatatcccaa cgtccgccgt aatcggcggc aatgctcagg gtcaggccgg
     9541 tattgtttgc cgtcaaggct tccgcctctt cgatgccttg cagaatctgc cgattaaagc
     9601 gttcgcggct gcccagtatt tttaggcgca tattgttttc gtgcagacgg cgtacctgtt
     9661 tttgcaaagc ctgtaaaaac agccccatca ggaacgaaac ttcgtcttcg gggcggcgcc
     9721 agttttcggt tgaaaaggca aacacggtca gatattgcac acccagtttg gcgcaatgct
     9781 tcaccatatt ttccaatgcg tccaaaccgc gtttgtgtcc cattatgcgc gggaggaaac
     9841 gttttttcgc ccaacggccg ttgccgtcca taatcacggc gatatgcttg ggaatggcgg
     9901 tgtgttccaa aacggcctgc gtgctgcttt tcatgtctgc ctttcgcggt tcggcattca
     9961 aatgccgtct gaacgccgaa ccgtgcaggt taaattgcca tcaaatcttc ttctttggca
    10021 gtcaggagtt tgtcggcttc ggtaatgtat ttgtcggtca gtttttgaac cgcttcttcg
    10081 ccgcgacgtg cctcgtcttc ggaaatttct ttgtctttga ggagtttttt gatgtggtcg
    10141 ttggcatcgc ggcgcacgtt gcggatagag acgcggcctt cttccgcttc gccgcgtacg
    10201 actttaatca ggtctttgcg gcgttcctcg gtcagcatgg gcatcggcac gcggatcagg
    10261 tcgccgacag ctgccgggtt cagccccaag tttgaatcgc ggatggcttt ctcgactttg
    10321 gccgccatat tgccctcaaa cggtttcacg ccgatggtgc gcgcgtccag aagcgttacg
    10381 ttggcaactt ggctgacggg gaccatgctg ccccagtatt cgacttccac ttggtcgagc
    10441 aggccggtat gcgcgcggcc ggtacgcact ttcgccagat tttctttcag tacttcgacc
    10501 gaacgctgca ttttgccttc ggctgttttt tgaatatcgt tgatcatatt gttctttcgg
    10561 tgggataagg tgggcgggca gccgtctgaa acgcgcttca aacctttcag acggcatgaa
    10621 aactgttaac tgcgaatagt accgttattc gggcatgacg acaaggcagg aggattggag
    10681 atgccgtctg aagcggcagg cgtttcagac ggcatcgtgt ccgaccgtca gccgtgttcc
    10741 cgtgtttcaa gcaggctttg gcgcaggtgt tggcgttcgt gggcatccag ccatttgcgg
    10801 cgggtgcgtt gcagcaggat gacgagggcg gaaatttcct gacgcatatt ggtgctgagc
    10861 cagaggaagc cctgccattg gtagtggagg tgttcggcga gggcttccag ttcggggttg
    10921 atggcggtgt cgatgcggat gcggcgggcg tgtctgccgt tgataagggc gacggtttgt
    10981 tgcaggtcgg tttggagcag tgtgaagtgg cggtcgagca gccggatttc gctgccgttg
    11041 agtttgggag attgcagctt ggcggcagtg gtcaggagca gctcggtggt gttgacaatt
    11101 ttacggtggg cgtgctgcat ggcttccatc atggcggggc tgatgcggct ttcgcccgat
    11161 gtggcggcga ggtggctgcg gcttttgacc atgcgtgcgt tgatttggcg cattttaacc
    11221 atattctgct ccaaacgttc gcgcgtcata cgcctgccgt tgctgatttc ggcaatcatt
    11281 ttgctgcagt cggtcaggtt gtcggcaagc atgaaacgcc acatcagtgt ggatttcagc
    11341 ggcagcagtt tggcggcggc aatggcgatg gccgcgccga tgaggacgtt catggcgcgc
    11401 atcaggccgc tgtcgaacca ttcgctgccg ttgtcgccga tgagcatgca catcgtcagc
    11461 cccgccagca tagggacgta gccgtttttg ccgaccgccg cccagccggc cagtgcgctt
    11521 gccgtgccga cggtgaggta gaagaggagg ttgccgtgga aataatgctg gttcagccat
    11581 aaaacgccca aacccgcgcc cagcccgatg accgtgccga gcatacgttc caccgccttg
    11641 gagtaaatcg ccccttgaaa ctggagcatg ccgaggacga cgaagacggt catccctatc
    11701 cactcgccgt gttggaggtg gagcagccgg gcggaggcgg tggcgaacag gacggccccg
    11761 ccgagccgga cggcgtggat gaggcggcgg tagcggtagc gttcgtagga gttgagccag
    11821 cggctgacga ggcggttgcg ttgcgaggtg ttcatatcgg ttgtgccgtc tgaagcggaa
    11881 atgtgaaaaa gcacaggctt cccgaggaag ggagggtctg tgcttggtat tggtgccgga
    11941 gaagggaatc gaacccccga ccttcgcgtt acgaatgcgc tgctctaccg actgagctac
    12001 accggcgttt tttcgtcatg atatatatga acggttgttt gtgcaacttt tcgggcgggc
    12061 ggcaaggcag tgcgcg
//
LOCUS       DAXHPG010000048        11850 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXHPG010000048 DAXHPG010000000
VERSION     DAXHPG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.048"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            26..1510
                     /gene="tldD"
                     /locus_tag="ACFM6F_000981"
     CDS             26..1510
                     /gene="tldD"
                     /locus_tag="ACFM6F_000981"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC6164419.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRMNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDSAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLMGTQSTGNGRRESYASVPMPRMTNTFMENGGYEPDEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     gene            1575..2036
                     /locus_tag="ACFM6F_000982"
     CDS             1575..2036
                     /locus_tag="ACFM6F_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164420.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAALWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAASAADGIKNDV"
     gene            2026..2847
                     /gene="prmC"
                     /locus_tag="ACFM6F_000983"
     CDS             2026..2847
                     /gene="prmC"
                     /locus_tag="ACFM6F_000983"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6164421.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            2933..4321
                     /locus_tag="ACFM6F_000984"
     CDS             2933..4321
                     /locus_tag="ACFM6F_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC6164422.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLVFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            4322..4546
                     /locus_tag="ACFM6F_000985"
     CDS             4322..4546
                     /locus_tag="ACFM6F_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC6164423.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(4944..5837)
                     /locus_tag="ACFM6F_000986"
     CDS             complement(4944..5837)
                     /locus_tag="ACFM6F_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6164424.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGAER"
     gene            5880..8582
                     /gene="ppc"
                     /locus_tag="ACFM6F_000987"
     CDS             5880..8582
                     /gene="ppc"
                     /locus_tag="ACFM6F_000987"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC6164425.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEELARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPQSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            8705..9694
                     /locus_tag="ACFM6F_000988"
     CDS             8705..9694
                     /locus_tag="ACFM6F_000988"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC6164426.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            9749..10078
                     /locus_tag="ACFM6F_000989"
     CDS             9749..10078
                     /locus_tag="ACFM6F_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164427.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            10089..10394
                     /locus_tag="ACFM6F_000990"
     CDS             10089..10394
                     /locus_tag="ACFM6F_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC6164428.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            10400..10579
                     /gene="ssrS"
                     /locus_tag="ACFM6F_000991"
     ncRNA           10400..10579
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFM6F_000991"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            10757..11188
                     /gene="rplM"
                     /locus_tag="ACFM6F_000992"
     CDS             10757..11188
                     /gene="rplM"
                     /locus_tag="ACFM6F_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC6164429.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            11198..11590
                     /gene="rpsI"
                     /locus_tag="ACFM6F_000993"
     CDS             11198..11590
                     /gene="rpsI"
                     /locus_tag="ACFM6F_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC6164430.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            11741..>11850
                     /locus_tag="ACFM6F_000994"
     CDS             11741..>11850
                     /locus_tag="ACFM6F_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6164431.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQ"
BASE COUNT         2788 a         3316 c         3257 g         2489 t
ORIGIN      
        1 tttttgttaa tccactatat aacaaatgcc gtataatcaa gcccctgatt ttttatgcgc
       61 cgacactatg catccgacct attccgccgt acaggcgcgg ctgctcgaag ccaaccgcct
      121 ttcccccgaa ctgctcgcca aaagcctgtg catcatcggc gcgcaccacg tcgattacgc
      181 cgacatctac tgccagcgca ccgcttatga aagctggcat ttggaagagg gcatcgtcaa
      241 atcgggcagc ttccaaatca atcagggcgt gggcgtgcgc gccgtttcgg gcgataaaac
      301 cgcctttgcc tacgccgaca gcctgtgcat cgattcgata aaccgttccg cccgcgccgt
      361 ccgcgcgatt ggggcggcag gcggcaaggt gtccgccaaa atgccgtctg aaacgcgcgg
      421 caagccggtt tgttccgcgt ccgaccccat tgccggcctc gattccgccg ccaaagtcgc
      481 gctgttgaac aaagtggaag caatcgccaa agccgccgat ccgcgcatcg tgcaagtgat
      541 ggccggtttg acctgcgaat acgacatggt ttacctcgcc cgtctggacg gtaaacacgc
      601 tgccgatatc cgcccgatgg tgcgcatgaa cgttaccgtt atcgccaaac agggcgaacg
      661 ccgcgaacag ggcagcgcgg gcggcggcgg acgctacgac ttggcttatt tcgatgaaaa
      721 cttggtgcat cggtttgtcg attccgccgt caaacaagcc ctgaccaatc ttgaatcccg
      781 tcctgcgcct gccggcgaaa tgaccgtcgt tttgggcaac ggctggccgg gcgtgttgct
      841 gcacgaagcg gtcggacacg gtttggaagg cgatttcaac cgcaagggaa ccagcgtctt
      901 ttccggccga atcggcgagc gcgtcgccgc caaaggcgtt accgttgtgg atcaaggcga
      961 tattgccggc cggcgcggtt cgctcaacat cgacgacgaa ggcaacgaaa cccgccgcac
     1021 cgtattgatt gaagacggca tcttggtcgg ttatatgcag gacgaaacca atgcccgcct
     1081 gatgggcacg caatcgaccg gcaacggccg ccgcgaaagc tacgcctccg tccctatgcc
     1141 gcgcatgacc aataccttta tggaaaacgg cggctacgaa cccgacgaaa tcatcgcgtc
     1201 catcgacaaa ggcatttacg ccgtcaactt cggcggcgga caggtggaca ttaccagcgg
     1261 caaattcgtg ttcagcgcgt ccgaagcgtg gtgggtggag ggcggcaggc tgcaataccc
     1321 cgtcaaaggc gcgaccatta tcggcaacgg ccccgaagtg ctgaaacacg tttccatgat
     1381 aggcaacgat actgcgctcg acagcggtgt cggcgtgtgc ggcaaagaag ggcagagcgt
     1441 ccccgtcggc gtggggcaac cgactttgcg gattgatgcc ggactgaccg tcggcggcag
     1501 cgcaatctga cgcggaatcc ggcacgatgc cgtctgaaag gttttcaggc ggccttttac
     1561 attaggataa aaccatgcag gaacagaatc ggaaatcaag ttttcccata gtgatgttgc
     1621 tggtgtcggt tgccctgtgg atagcgtctt tatccaatgt tgcattttat ttgggcaatc
     1681 atggaagcat ggagggtttg accgttttga ttttggggtc gatatttgct tctttggata
     1741 tcaggtattg tgcggtctat gcgaattatg tttggttggc ggccattgtt ttgctggcgt
     1801 tgcggaagaa ggtcgtgcct gtccatgcgg cactttgggg cttggcgttg gtggctttca
     1861 gtgtgaaagc cgtatacgtc gatgaagcag ggaatacatc ggatattgtg cgctacggtg
     1921 caggatttta tttgtggtat gccgcatttg cggttgccac catcggtacg tttgccggaa
     1981 agaataagga aagaaaagcc gcatcggcgg cagacgggat aaaaaatgac gtttgacgaa
     2041 tggttgggct tgtcaaaact gcctaaaatt gaagcaagaa tgctgctgca atatgtttcg
     2101 gaatatacgc gcgtgcagtt gttgacgcgg ggcggggaag aaatgccgga cgaagtccga
     2161 cagcgggcgg acaggctggc gcaacgccgt ctgaacggcg agccggttgc ctatatttta
     2221 ggtgtgcgcg aattttatgg cagacgcttt acagtcaatc cgagcgtgct gattccgcgc
     2281 cccgaaaccg aacacttggt cgaagccgta ttggcgcgcc tgcccgaaaa cgggcgcgtg
     2341 tgggatttgg ggacgggcag cggcgcggtt gccgtaaccg tcgcactcga acgccccgat
     2401 gcgtttgtgc gcgcatccga catcagcacg cccgcccttg aaacggcgcg gaaaaatgcg
     2461 gcagatttgg gcgcgcgggt cgaatttgca cacggttcgt ggttcgacac cgatatgccg
     2521 tctgaaggga aatgggacat catcgtgtcc aacccgccct atatcgaaaa cggcgataaa
     2581 catttgttgc aaggcgattt gcggtttgag ccgcaaatcg cgctgaccga cttttcagac
     2641 ggcctaagct gcatccgcac cttggcgcaa ggcgcgcccg accgtttggc ggaaggcggt
     2701 tttttgttgt tggaacacgg tttcgatcag ggcgcggcgg tgcgcggcgt gttggcggag
     2761 aatggttttt caggagtgga aaccctgccg gacttggcgg gtttggacag ggttacgctg
     2821 gggaagtata tgaagcattt gaaataattg tttgcaaaat tggcgggttt tccattattg
     2881 ggttatgatg tgtttcgata tgtaaacatt tataggtttg gagcgataaa caatgaatgc
     2941 agtcgtagtt gccgtaatcg ttatgctggt gctgtcgctg tcgcgcgtgc acgtggtatt
     3001 gagcctgacg gtcggcgcgt ttgtcggcgg cgcggtggcg ggtatgccgc tgcaaaacat
     3061 tgccgatgcg gcgggacagg tcagtcaggc ggggattatc cccgtgttca acaaaggttt
     3121 ggaaggcggt gcgaagattg cgctttctta tgcgatgctc ggcgcgtttg caatggcgat
     3181 tacccattcc ggcctgccgc aacagcttgc cggcgcggtc gtccgcaagc tgaaccgggg
     3241 cggtatgccc gacagcgtgc gttcgggcga gggcgcggtc aaatggctgc tgctttccat
     3301 catccttgtg atgggcatta tgagtcagaa catcatcccc atccacatcg cctttatccc
     3361 gatgattgtt ccgccgctgc ttttggtgtt caaccgcctg aaaatcgacc gccgcctgat
     3421 tgcgtgcgtc atcactttcg ggctggttac gacttatatg ttcctgcctt acggcttcgg
     3481 cgcgattttt ttgaatgaaa tcctgttggg caacatccat tccgccgcgc cgcagcttga
     3541 tgtgaaaaac atcaacgtaa tggcggcaat ggcgattccc gcgttgggaa tgctggccgg
     3601 actcctgctg gtgtttgtcc attaccgcaa accgcgcctg taccaaagca acaatgccga
     3661 tacggcgggc aacgccgatg cggcaaaccg tccgcagccg tccgcctacc gcagcctggc
     3721 cgccgccgcc gccattgccg tatgctttgc catccagttg atgtatgaag actcgctggt
     3781 gttgggcgcg atgctcggtt tcgccgtatt tatgatgttg ggcgtcatca accgcgacaa
     3841 ggcaaacgac gtattcggcg aaggcatcaa gatgatggcg atggtcggct tcattatgat
     3901 tgccgcgcag ggttttgccg ccgtgatgaa tgcgaccggg catattcagc cgctggtgga
     3961 aagcagtatg gcgatattcg gcaacagcaa aggtatggcg gcgttggcga tgctggtggt
     4021 ggggcttttg gtaacgatgg gcatcggttc gtccttttcc actttgccga ttattgccgc
     4081 gatttatgtg cctttgtgtg tcggtttggg tttttcgccg cttgccaccg tcgccattgt
     4141 cggcacggcg ggggcgttgg gcgatgccgg ttcgcctgcg tccgattcca cgctgggccc
     4201 gacgatgggg ctgaacgccg acgggcagca cgaccacatc cgcgattccg ttatcccgac
     4261 cttcatccac tacaacatcc cgctgctgat tgccggctgg attgccgcga tggtgctgta
     4321 aatggacgcg gttcaagagt tggaacgccg tattgtcgaa ctggaaatcc aatccgcgct
     4381 tcaggaggac gtaatcgccg gcctgaacgc gatggtggcg gaattgcggc agacgctgga
     4441 tttgcagcag gctcagttga ggctgctgta tcaaaaaatg caggacagga atcccgacgc
     4501 gcaagagccg tattccctgc gcgacgagat tccgccgcat tattgatgcg ccgccgtatc
     4561 cggatttcct taaaaaaggc tgtgtttgaa tattttgccg atgcaaacct aagatgtaac
     4621 tttaatcagt cggacaaaat gccttttatc cgatggaacc gttttccgcc cgaacggaaa
     4681 taagcggttt tcccctgtaa acagataaaa ccaaccgggt attcatacac agccaaaaaa
     4741 aagccgtccg aacccaaagg ggacagacgg ccaaacacat tacggggaaa acgtcttact
     4801 caatgagtct gcgaaacaga cattgctaaa acaactgcaa ttataaggta agtttatttt
     4861 tttgcaaaga aatgtaaatt tttaaatttt taaaaccgtt ccgccgcatc cgtttggata
     4921 ccgtctgaaa cggcggcagg gttttaccgt tccgcgccgc aaatcgggca ttcggggttg
     4981 cgcggcaggt cgaaatattg ccagccccct tccaaggcac ggtaaaccgc cagcctgccg
     5041 tgcgacggtt cgcccgcatc cagcaggatt ttcagagcct ccgccgcttg ggtactgccg
     5101 atgatgccga ccagcggcga gaacacgccg aagagggaac agatgccgtc tgaagccgat
     5161 ccgccgtcaa acaggcaggc gtaacacggc gagtcgggca agtcgggacg gtacacggca
     5221 agttgccctt caaagcgcac cgctgctcct gaaaccagcg gtgttttcgt ttgtacgcag
     5281 acacggttga cggcttggcg cgtggcgtag ttgtcgcagc agtctaaaac gatgtcggcg
     5341 gtttgaacca aaccggtcag gcggcagccg tcgagttttt cgttgacggt gcggacatcg
     5401 acgttatggt tgatgcgttt caggcggtct gccaaggctt cggctttggg cttgccgaca
     5461 tcgccctcgt caaatgcgac ttggcgttgc aggttgtgca gctcgaccgt gtcggaatcg
     5521 gctatggtca gcgtgccgat gccggaagcg gcgagatagg gcagggcggc ggcacccaaa
     5581 ccgccgcagc cgacgaccaa aatatgcgcg gcggaaagtt tctgctgccc ttcgatgccg
     5641 atttcgtcca agaggatgtg gcggctgtac cgcagcagga atgcatcgtc gttgtcgtgt
     5701 tcggtcgtgg tcatgatgat gttcggaaaa aacagttgtg ggcgattgta acgctgccgt
     5761 cgggcggcgt tcaacttcag acggcatttc gggacacggg cggttaaagt gtgaacggtt
     5821 tggcacggat gcggcatttg aggtacattt acagtattta cggcaatgag agaaaaatca
     5881 tgcaactgca tattctgaac aatccaaagg acgcggcttt ggcggcggac gcggaatttc
     5941 tgaaacaatc cctgttcaac ctcctgcacg aagaagcctc gccattggtt gtcgaaacgg
     6001 tcaaactctt gtccacttcc gacgacagcg cggcattgat tgaaaaagta ttgccgcaat
     6061 tggacgaaca acaaacccac gatttaacct tggcctgcgg cctgttcgcc cagattttga
     6121 atatcgccga agacgtgcac cacgaacgcc gccgccaaat ccacgaagaa gccggacgcg
     6181 gcggcgcgga aggcagcctg acggaaaccg tccgcaggct caaagcgggg aaagccgacg
     6241 gcaaatcggt gcagcggcag ttggacaata cgtccgttac cgccgttttg accgcgcacc
     6301 cgaccgaagt gcaacgccaa accgtcttaa gcttcaaccg ccgcatccgc gcactgttgc
     6361 cgcaacgcga acgctgcacc aatgccgacg cgctggcacg gctgcgccgc gaaatcgaca
     6421 ctatcctgct gggcttgtgg cagaccagcg aaacgcgccg ccacaaactc agcgtcaacg
     6481 acgaaatcaa caacggcgtg tccatcttcc cgatgagctt tttcgaagcc ctgcccaagc
     6541 tctaccgcaa tatggaacac gactttcaga tggtctatcc cgatgtctgc gttccgaaca
     6601 tcctcaaaat cggcggctgg atcggtggcg accgcgacgg caatccgttc gtttctgccg
     6661 aaaccttgcg ttttgccttc cgccgccacg ccgatgccgt gttccgtttt taccgtggcg
     6721 aactcgacaa actttaccgc gaactgccgc tctccatccg ccgcgtcaaa gtcaacgacg
     6781 acgtaatggc actggcagcc ctctcgcctg acgaagaact tgcccgcacg gaagaaccct
     6841 accgccgcgc cattgcttac atcatggcgc gcgctatggg caaagcgcgc gcgctcggtt
     6901 tgggcatggg ctgcaaattc ggctttctcg agccttatgc ttcggcacaa gaatttctcg
     6961 atgatttgaa aaaattgcaa cgttccctta tcgacaacgg cagccgcctg cttgccgaag
     7021 gccgtctggc agacctcatc cgttccgtgt ccgtgttcgg cttccacatg atgccgctcg
     7081 acctgcgcca acacgcaggc aaacacgccg atgtggttgc cgagcttttc caacacgcag
     7141 gcttggaaga ctacaacagc ctgaatgaag agcaaaaaca agccgccctg ttgcgcgaat
     7201 tgagccatca acgtccactg tacagtccgt tcatcacata cagcgaccat actcgccacg
     7261 aactggcgat tttcaatgaa gcgcgcaaaa tcaaagacga attcggcgaa gatgccgtta
     7321 cgcaaagcat tatttccaac tgcgaacaac ccagcgacct gctcgccttg gcattgctgc
     7381 tgaaagaaag cggcctgttg gcggtagaaa acggcaaacc gcaaagccgc atcaatatcg
     7441 tgccgctgtt tgaaaccatc gaagcgttgg aaaacgcctg tccggtcatg gaaaccatgt
     7501 tccgcctcga ctggtacgat gccctgctcg aaagccgcgg caatatccaa gaaatcatgc
     7561 tgggctactc cgactccaac aaggacggcg gctacgttac cagctcatgg tgcctctatc
     7621 aggcggaatt gggcttggtc gaactcttca aaaaatacga tgtccgcatg cgcctgttcc
     7681 acggacgcgg cggcagcgtc ggacgcggcg gcggcccttc ttaccaagcc attttggcgc
     7741 aaccggccgg cagcgtggcg ggacaaatcc gcattaccga acaaggcgaa gtcattaccg
     7801 ccaaatacgc cgaccccggc aatgcccaac gcaacttgga aaccttggtt gccgcgactt
     7861 tggaagccag catcctgccg gataaaaaag accctgatgc caaactgatg caggcattgt
     7921 cggacgtatc gttcaaatac taccgcgaac tgattaccca ccccgacttc atcgactact
     7981 tcctgcaaac cagcccgatt caggaaatcg ccaccctcaa cctcggcagc cgtcctgcca
     8041 gccgcaagac cttagcgcgg attcaagact tgcgcgcgat tccgtgggta ttttcctgga
     8101 tgcaaaaccg cctcatgctg cctgcttggt acggtttcgg cagcgcggtg gaaaccttgt
     8161 gcgaagacaa acccgaaacg ctcgccgccc tgcgcgaaca cgcccaaagc aacccgttct
     8221 tccaagccat gctttccaat atggagcaag tgatggcgaa aaccgacatc accctcgcgg
     8281 aaaactatgc cggcttgagc gaatcgccgg aaaaagccgc cgtcatcttc ggcatgatta
     8341 aggaagaata ccaacgcagc cgcaaagccc tgctcgacct gctgcaaacc gaagaacttt
     8401 tacgcgacaa ccgcagcctc gcccgttcgc ttgccttgag gattccctac ctgaacgcgc
     8461 tcaacggcct gcaggtcgcc atgctcaaac gcctgcgtaa agaacccgac aatccgcacg
     8521 cgctgctgat ggttcacctg accatcaacg gcgtggcgca aggtttgcgc aatacaggct
     8581 gattgtgccg catcggggca aaatgccgtc tgaacacctt tcagacggca tttccctgac
     8641 cgcacttgca gagaaacacc gattgtttta aagtgaacgg cagtgatatg ttgaaagacg
     8701 accaatgaaa attaccgtta tcggcgcagg ttcgtggggt acggcgctcg ccctgcactt
     8761 ttcccaacac ggcaaccgcg tatccctgtg gacgcgcaac gcagaccaag tccgccaaat
     8821 gcaggaagcg cgtgaaaaca aacgcggact gcccggcttt tcctttcccg aaaccttgga
     8881 agtgtgtgcg gatttggcag acgcgctcaa agacagcgga cttgtcctta tcgtaacctc
     8941 cgttgccgga ttgagaagca gcgcagagct gctcaaacag tacggcgcgg gacacctccc
     9001 cgtcctcgcc gcctgcaaag gattcgagca ggatacaggg ctgctgacct ttcaagtatt
     9061 gaaagaagta ttgcccgaca ataagaaaat cggcgtactc tccggcccga gttttgcaca
     9121 ggaactcgcc aaacaactgc cctgtgccgt cgtcctcgcc tccgaaaacc aagagtggat
     9181 tgaagaactc gtaccgcagc tcaacacgac tgtcatgagg ctttacggca gtaccgacgt
     9241 tatcggcgtg gcggttggcg gcgcggtaaa aaatgttatg gcgattgcca ccggattgtc
     9301 cgatggccta gagtacgggc ttaacgcccg tgccgcactg gttacgcgcg gattagctga
     9361 aattacccgc cttgcctccg caatgggcgc acagcccaaa accatgatgg ggctggcagg
     9421 catcggcgac ctcatcttga cctgcactgg cgcactctca cgcaaccgcc gcgtcggctt
     9481 gggtttggca gaaggcaagg aactgcatca ggtgctggtc gaaatcggac acgtttccga
     9541 aggggtcagc acgatagaag aagtcttcaa tactgcctgt aagtaccaaa tcgatatgcc
     9601 gattacccaa accctgctgc aactcatccg taaggaaatg acccctcagc aggtcgtcga
     9661 aagactgatg gaacgcagcg cgcgtttcga ataaacaaca gacagatgcc gtctgaggcc
     9721 ttcagacggc atacggacag gtaaggttat gaaacaaaat atcgaaaaac tcgaaagcag
     9781 cgtttatacg ctggtacaaa aattcgaaac cctcgtcagc gaaaaccgcc gcctcaaaga
     9841 aaccgtcgcc gaactcaaac gggcgcacga gcggcaaaaa ctcgaacacg aaaccgccgt
     9901 cgacgaactc agcgaagccc tgctcgtcca agtcggcaaa ctcaaagaag acctgcaaaa
     9961 caaaattgac agcctgacag aagaaaatac acgataccgc agcctgctcg aacagagcag
    10021 ggaaaaaatc agcgcactgg cagcgcgcct cccccaatgg caggaaacgc agcaataagg
    10081 attaaaggat gaacatcgaa caagtccaca tcgaagtcat gcacgcccgg ctgaccgtca
    10141 acacgccggc agaagaaaaa gacacactgt tgcaggcagt cggaatgctc aacggcaaag
    10201 ccgaagccat ccgcgaaggc ggacgcgtcg cggacagcga aaaaatcgtc attatggccg
    10261 cgctcaacgt cgtccacgac ctgttgaaaa cctccctgaa cggcggcgat ttggcaatcg
    10321 gcgattttgc gcgtaaaata gccgatatgg acaacgcctg ccaaaaagca ctatcccgct
    10381 tggcgcagga ataacccctt tttccctgcg gtgtccgcga aggcatatat tcctttgaac
    10441 caataagttc gcttaaggtt gccgggagca gtagtgggcg cgagcgtcct tttgcggacg
    10501 cacccgaaac tacccgaggc agccgccttg tcagcaaggt tcaagcggat tcggccgaaa
    10561 cggctctcgc ggggattccc attcaaaacc gcatcgcaac gatgcggttt ttcacattgg
    10621 ccgccgcccc gcccgaaaaa gcagtatgca gtctgccgaa atccggaaaa tccttccgta
    10681 acgcggtaat gcgattgaca aaatcgaatc ctgtctttaa aatttacaac tttctatatc
    10741 tatttggatt tccactatga aaaccttttc agcgaaaccc cacgaggtga agcgcgaatg
    10801 gttcgtcatc gatgcccaag acaaagtctt gggtcgcgtt gcggccgaag tcgccagccg
    10861 tctgcgtggc aaacacaaac ctgaatacac cccccacgtc gataccggcg attacatcat
    10921 tgttatcaat gcggacaaac tgcgtgtaac cggtgccaaa ttcgaagata aaaaatactt
    10981 ccgccattcc ggttttccag gcggtatcta cgaacgcact ttccgcgaaa tgcaagagca
    11041 attcccgggc cgcgctttgg aacaagctgt aaaaggtatg ctgcccaaag gtcctttggg
    11101 ttacgccatg attaaaaaac tgaaagtgta tgcgggtgcg gaacacgccc atgctgcgca
    11161 acaacccaaa gttttggaac tgaaataagg acgcgacatg aacggtaaat actactacgg
    11221 cacaggccgc cgcaaaagtt cagtggctcg tgtattcctg attaaaggta caggtcaaat
    11281 catcgtaaac ggccgtcccg ttgacgaatt cttcgcacgc gaaaccagcc gaatggttgt
    11341 tcgccaaccc ttggttctga ctgaaaacgc cgaatctttc gacatcaaag tcaatgttgt
    11401 tggcggcggc gaaaccggcc agtccggcgc aatccgccac ggcattaccc gtgccctgat
    11461 cgacttcgat gccgcgttga aacccgcctt gtctcaagct ggttttgtta cccgcgatgc
    11521 ccgcgaagtc gaacgtaaaa aaccgggtct gcgcaaagca cgccgtgcaa aacaattctc
    11581 caaacgttaa tgttggaaat tcaaaaaacc ctgcttatcg cagggttttt tatttgtagt
    11641 agacggtttc ccattggcaa tctaaagatt acagattgag acctttgcaa aattccccaa
    11701 aatcccctaa attcccacca agacatttag gggatttctc atgagcacct tcttccagca
    11761 aaccgcccaa gccatgattg ccaaacacat cgaccgtttc ccgctattga agttggatca
    11821 ggtgattgat tggcaaccga tcgaacagta
//
LOCUS       DAXHPG010000049        11816 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXHPG010000049 DAXHPG010000000
VERSION     DAXHPG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.049"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(53..2353)
                     /gene="recQ"
                     /locus_tag="ACFM6F_000995"
     CDS             complement(53..2353)
                     /gene="recQ"
                     /locus_tag="ACFM6F_000995"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC6164432.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSSTSADGAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFISSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     TREDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLCY
                     GLNDWVLLHERIAEGNSDEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSNKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNAADFSHDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKHKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
     gene            complement(2418..3200)
                     /gene="trpC"
                     /locus_tag="ACFM6F_000996"
     CDS             complement(2418..3200)
                     /gene="trpC"
                     /locus_tag="ACFM6F_000996"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC6164433.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRAGDIEAEVGKLF"
     gene            complement(3250..4206)
                     /locus_tag="ACFM6F_000997"
     CDS             complement(3250..4206)
                     /locus_tag="ACFM6F_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HFC6164434.1"
                     /translation="MILTPPDTPLFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRDRGWHGVVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTFAARYREIYAVGVSLGGNALAKYLGEQGKKALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKAKSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPIPDEVSEAV
                     TLFQPAHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNSR"
     gene            complement(4339..5877)
                     /gene="murJ"
                     /locus_tag="ACFM6F_000998"
     CDS             complement(4339..5877)
                     /gene="murJ"
                     /locus_tag="ACFM6F_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC6164435.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEAAEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAQDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFGIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQVSLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRKHGIYRPGKGWAAFLAKMLL
                     ALAVMGGGLYAAQIWLPFDWTHTGGMQKAARLFILIVIGGCLYFASLAALGFRPRHFK
                     RVES"
     gene            complement(5877..6068)
                     /locus_tag="ACFM6F_000999"
     CDS             complement(5877..6068)
                     /locus_tag="ACFM6F_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164436.1"
                     /translation="MLEINRNPAILLHFRVLIQFAAGKGRFRFGKPLQTASNPMPSEA
                     VSVVQYAPLPQTGTTVEEQ"
     gene            6095..6793
                     /locus_tag="ACFM6F_001000"
     CDS             6095..6793
                     /locus_tag="ACFM6F_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6164437.1"
                     /translation="MKSRQLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(6850..7854)
                     /gene="amgK"
                     /locus_tag="ACFM6F_001001"
     CDS             complement(6850..7854)
                     /gene="amgK"
                     /locus_tag="ACFM6F_001001"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC6164438.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDFGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            7924..10332
                     /locus_tag="ACFM6F_001002"
     CDS             7924..10332
                     /locus_tag="ACFM6F_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC6164439.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMEIEQGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            10437..11489
                     /locus_tag="ACFM6F_001003"
     CDS             10437..11489
                     /locus_tag="ACFM6F_001003"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6164440.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKVAKVAKVAKVAKV
                     AKVAKVAKVAATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLP
                     KEAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSPAQR
                     RDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILI
                     KADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPA
                     FEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQAT
                     VNLLRDLHSGAYVDIR"
BASE COUNT         2718 a         3397 c         3316 g         2385 t
ORIGIN      
        1 aaacaggaat ttttaaatag aggcaatgcc gtctgaaact tggtaacggg cttcagacgg
       61 catttcgttc caataccgcc aacaccgccg caccgtaacg tgcggctttt tcttcgccta
      121 cgccatatac ggcggcaagc tccgccaaac cttccggctg tttggcggca atggcgcgca
      181 gggcggcttt gctgagaatg cggtagggtt cggactgttc gtgttttgcc gtttcgccgc
      241 accattgaat cagggcgcgc atcaggcggc gtttgtgttt ggcggcttca tcgatgccgt
      301 ctgaaaacgg acggcagacg gcgaggatgt cccgtccgta tttggcggcg cgtacgctgc
      361 ccaagccgtg cacgccttcg aggtcggttt cggtttcggg cgtggcggca agcatatcgg
      421 caaggctttc gtcggagagg acggcatgca gggcgcagtt ttccgccctt gcctgttcat
      481 accgccaggc ttcgagtttt tgacgcagtt gttgttcgcg ttcggtttgc ggacggatga
      541 cggcatcgtg gctaaagtcg gcggcgttgc ggcagacttc gaggataccg tgtccgaaac
      601 ggtcgatttt ggcttcgccc aagccgtaga tgtcgtgcag accgttgagg tcttgcggca
      661 ttttttcgac aaggtcgcgc agggttttgt cgccgaaaat catataggcg gggatgcctt
      721 cggcttctgc ctgtttcata cgccaaacgc gcaatgcctg ccacaggcgt tcttcgcgtt
      781 cggtacgcag ccagttgtct ttgagggtgc gggcggcggg cttgtcgcgc ttgagcggac
      841 gcagcatcac ttcggtttcg cctttgagga cttttttggc ggcttcggtc agttgcaatg
      901 cctgatatcg ggtaatgttg acggtgaggt agccgaggct gatacactgg cggatgacgc
      961 tgcgccattc tttgttggac aactccgtac cgatgccgaa tgtggacagt tgttcgtgcc
     1021 ggttgccgcg tatccaatcg tcgcttttac ctcgcaaaag gttggtgatg taaccggcgg
     1081 caaaacgttg tccggcgcgg tacacgcagc tgagtaattt ttgcaccaac accgtgccgt
     1141 caaaccgtac gggcggatgc aggcagttgt cgcaatggcc gcagggttcg gatgcttcgc
     1201 cgaaatgttt gagcagcagt acgcggcggc aggcggcggt ttcgcagacg gcaagcatgg
     1261 catcgagttt ttgcatttcg atttgctttt gcacctcgtc gctgttgcct tcggcaatcc
     1321 gttcgtgcag caacacccaa tcgttcaaac cgtaacacag ccaactcacg gcaggcagcc
     1381 cgtcccgtcc ggcgcgcccc gattcctgat agaaatgttc gacactctgg ggcatatcga
     1441 gatgggcgac aaagcgcacg tcgggtttgt ctatgcccat accgaacgcc acggtcgcca
     1501 ccacgataat attgtcttca cgcgtaaagc ggcgttggtt ttcctcgcgc acgtccatgc
     1561 tcaaaccggc atgatacgga atcgcgttta atccgttttc acgcaaaaac tgcgccacat
     1621 cttcgacttt tttgcggctt aggcaataca caatgccgct ttgccccgtc atttctttgc
     1681 ggatgaattc aagcagttgt tttttgccat tatttttttc gataacctga taataaatat
     1741 tcgggcggtc aaagctggag ataaattcgg gcgcatcgtc caagtgcaga taatgcttga
     1801 tgtcggcgcg cgtggcggca tcggcggtgg cggtcagggc gatgcgcggg acgttcggat
     1861 agcgttcggc aagcatgccg agctgttggt actcggggcg gaaatcgtgt ccccattggc
     1921 tgacgcaatg cgcctcatca atggcgaaca ggctgacggt ttgttggtcg agaaaacgca
     1981 aaaagcggtc ggtaaccaag cgttccggcg cgacataaag cagtttcaga cggccttggg
     2041 caagcttgtc ggcaatctcg cgcgccccat ctgccgatgt gctgctgttg actgccgccg
     2101 cttcgatgcc ggcggcgtgc aggtttgcca cttggtcgtt catcaacgca atcagcggcg
     2161 atacgacaac cgccacgcct tcgcgcatca gcgcgggaat ctggtaacac aaagacttgc
     2221 cgccgcccgt cggcatcagc accgtcaaac tcccgccgcc tgccaaagta ttgatgacag
     2281 cctcctgcct gccgcgaaat tcgggataac caaatacttc gtgcagaatc tgtttggcgg
     2341 tcggtcggtg catgatggtt ccgtgctcgg taagggtgtt ggtcggttgg cggcaatatg
     2401 ccgtctgaaa tcgggattta gaagagtttg cccacttccg cctcaatatc gccggcacgc
     2461 atgaacgttt cgccgatcag gaaggtgtgc acaccgcgcg attgcataaa ttccacatcc
     2521 gccttgcctg taatgccgct ttcggttacg acggttttgc cttccagcgc gggcagcagc
     2581 gacagggttt ggtcgaggga gacttcaaaa gtcctcaggt tgcggttgtt tacgccccac
     2641 aacggcgtgg tcaggttgcg gcatttttcc aattcggttt catcgtgcag ctcgagcagg
     2701 acggtcatgc ccaattcgtg cgccaccgct tcaaagcgtt ccaattgttc ctgttccagt
     2761 gctgcggcaa tcagcaggac ggcatccgcc ccccatgcgc gcgcctgata aacctggtat
     2821 tcgtcgatga tgaagtcttt gcgcagcacg ggcagcgata cggcttcgcg cgcctgtttg
     2881 aggtattcgg gcgaaccttg gaaatagggt tcgtcggtca gtacggacaa acacgccgct
     2941 ccggcgtttt cataggcgcg tgcaatctcg gcagggcgga agtccggacg gattaaccct
     3001 ttgctcgggc ttgccttttt gatttcggct atgacggcgg gcaggtttag gcggtgtttg
     3061 ccgcgtatcg aatcgatgaa gctgcggacg ggcgcggctt ctgcggcaag tgcgcggatg
     3121 tgttcggcgt tgacggcggc tttttgagcg gcaacttcct gtgctttggt ggcaaggatt
     3181 ttattgagga tgtcggtcat gtcgggttcc gtattcgtct gggaaagggg gaatattagc
     3241 atcaaaccgt taacgactgt ttgtgcggaa gctgtcgaaa taggacagga cggtctgcgg
     3301 caaccattgc aggtgcagcc tgccgccggt gctgccgaca aagccgacat gaccaccgtg
     3361 tgcaggttgg aacagggtaa cggcttcgga cacttcgtcc ggtatcggca gggcttcggg
     3421 cggcaggaag gggtcgttga cggcattgag caggagcagc ggtttggcaa cgtgtttgag
     3481 cagcggtttg caggaagttt ggcggtagta gtcgtgccgg tcggcaaagc cgtgcagcgg
     3541 tgcggtgaag cggtcgtcaa actcgcccag tgttttgcac cctgcggcaa atgccgtctg
     3601 aaaaccttgg agcgattttg ctttgggtat cagggtgcgg aggaagtagc gcgtgtagag
     3661 cagccgcgtg atgccgctgt cgaagcgtct gcctgccgcc tctgcatcga cgggggcgga
     3721 gatgacggca gcggcttgcg gcaatgcctt tttgccctgt tcgcccaaat attttgccag
     3781 cgcgttgccg cccagcgata cgccgacggc gtatatttca cggtaacgcg cggcgaacgt
     3841 gtccaaagta aaggcgattt cggcggtatc gcccaagtgg tagaacaccg gagcggtgtt
     3901 ggcaatgccg ccgcagctgc ggaaatggac gactacgccg tgccaacccc gatcgcgtac
     3961 cgcaagcatc agttcgaccg cgtaatggct gcggctgctt ccttccaaac cgtgaaacag
     4021 cacgaccagc ggcgcatcgg gcgaaatgcc gtctgaaaag tcgtaggcga ctttggtttt
     4081 acccgtgctg tcgggaagca gctcgcggcg gtatgcaggt gcggggcgtt gcaggaattt
     4141 ggcggcaatc gtgtcggcat tgccgttgcg gaggaaaagg ggcgtgtccg gcggtgtcaa
     4201 aatcataagg tatcggtttt cttgttttca gacggcattg atgatgcggc agcccgtccg
     4261 gccggtgcgg acgcggggga tgcgcgcccg aatataggcg tggaaaaagc gtttgccgaa
     4321 aaaggatatc ggcatcggtc agctttccac ccgtttgaaa tggcgcggac ggaagcccag
     4381 tgcagccagt gatgcgaaat acaggcagcc tccgatgaca atcaggatga agagccgggc
     4441 ggccttttgc attccgccgg tgtgtgtcca gtcgaacggc agccagattt gggcggcata
     4501 caggccgcct cccatcacgg cgagcgcgag cagcattttt gccaagaacg ccgcccaacc
     4561 cttgccgggc cggtaaatac catgtttgcg caacaggtaa aacaacaatc cggcattgat
     4621 acacgcgccc agaccgatgg caagcgaaag tccgacgtgt ttcagtgggc cgataaaggc
     4681 aaggttcatc aactgcgtgc aaatgagcgt gaagatggcg attttgacgg gcgttttgat
     4741 gttttgccgc gcataaaagc cgggcgccaa cactttaatc atgattaaac cgattaaacc
     4801 gaaagaatag gcaatcagcg cgtgttgcgt catttgcgcg tcaaacagcg tgaattcgcg
     4861 gtacataaac agcgtcgcca ccagcgggaa cgacaacacc gccagtccga ccgccgccgg
     4921 cagcgtcagc agcatacaca ggcgcaaacc ccagtcgagc agggcggaaa actgttccgt
     4981 atcttggttt gccgagtgtt tggacaaagt cggcagcaaa atcgtaccga gtgccgcccc
     5041 cagcacgccg ccgggcagtt ccatcatgcg gtcggcgtaa tacatccatg aaacgctgcc
     5101 cgattgcaga taagacgcga aaatcgtgtt gatcaccaaa gaaacctgcg ccacgctcac
     5161 gcccaaaatc gccggcgcca tctgtttcat cacgcggttg accgccgcat ctttgaaatt
     5221 cagtttgggc agtttcaaaa agcccagttt cgccagccag ggcagttgga agccgagttg
     5281 caaaataccg ccgacaaaaa ccgcccatgc cagagcggta acgggcggat cgaaatacgg
     5341 cacgaaaaac agcgcgaata cgataaacga cacgttcagg aatgtgggcg taaacgcggg
     5401 aatgccgaac ttatgatagg aattgagtac cgagccgaca aaagaggaaa gtgaaatcaa
     5461 taggatataa ggaaacgtaa tccgcagcaa atcgatggaa agttggaact tgtccgcgtc
     5521 ttgggcaaag ccgggtgcgg aaacataaat cacccaaggc gcggcgagta tgcccagcgc
     5581 ggtaacgatg accagcacaa acgacagcat ccccgccaca tggcggataa aagcctccgc
     5641 cgcctcttta gaacgcgttt ccttatattc cgccaaaatc ggcacaaacg cttgggcaaa
     5701 cgccccctcc gcaaacacgc ggcgcagcag gttgggcagt ttgaacgcga caaaaaacgc
     5761 atccgtcgcc atacccgcgc cgaatgcccg cgcaatgacc gtatcgcgca caaatcccaa
     5821 aacgcgcgac accatcgtca ggctgccgac ttttgccaaa gctcccagca tattcatcat
     5881 tgttcctcaa cagtcgtacc cgtttggggc aacggcgcgt attgtacgac agaaaccgct
     5941 tcagacggca tcgggtttga tgccgtctga agcggtttcc cgaaacgaaa acgtcctttt
     6001 ccggcggcaa actgtatcaa cacgcggaaa tgcaataaaa tagccggatt ccgattgatt
     6061 tccaacatct gtttccaaca tcacggagag ccgtatgaaa tccagacaac tcgccctcgg
     6121 cgttgccgcc ctgttcgccc ttgccgcgtg cgacagcaaa gtccaaacca gcgtccccgc
     6181 cgacagcgcg cctgccgctt cggcagccgc cgccccggca gggctggtcg aagggcaaaa
     6241 ctataccgtc cttgccaacc cgattcccca acagcaggca ggcaaagtcg aagtccttga
     6301 gtttttcggc tatttctgtc cgcactgcgc ccacctcgaa cctgttttga gcaaacacgc
     6361 caagtctttt aaagacgata tgtacctgcg taccgagcat gtcgtttggc aaaaagaaat
     6421 gctgccgctg gcacgcctcg ccgccgccgt cgatatggct gccgccgaca gcaaagatgt
     6481 ggcgaacagc catattttcg atgcgatggt caaccaaaaa atcaagctgc aagagccgga
     6541 agtcctcaaa aaatggctgg gcgaacaaac cgcctttgac ggcaaaaaag tccttgccgc
     6601 ctacgagtcc cccgaaagcc aggcgcgcgc cgacaaaatg caggagctga ccgaaacctt
     6661 ccaaatcgac ggtacgccca cggttatcgt cggcggtaaa tataaagttg aatttgccga
     6721 ctgggagtcc ggtatgaaca ccatcgacct tttggcggac aaagtacgcg aagaacaaaa
     6781 agccgcgcag taagcccgtt tgaaaaatgc cgtctgaaac ttggttttca gacggcattt
     6841 tgattgggtt taaaacgtaa agcccgtttc cagttcttca tcgccgacca gttcgaccaa
     6901 gagcgcgtag agcggggcga gttcggcata acggcgcgat acgcggcgca ggtaattgag
     6961 gaaacgcggg atttcgggac ggtatttgtc tttgccgtcg cggtagtaca ggcgtgcgaa
     7021 gatgcctgca accttcaagt gccgctgcac gcccatccat tcgaaccagc ggtaaaactc
     7081 gtcaaacgct tcggggacgg gcaggccggc agcccgcgcc ttttcccagt agcggataac
     7141 caagtccaag acaaattctt cttcccattc gataaaggca tcgcgcaaca gcgacaccaa
     7201 atcgtaggaa atcgggccgt aaagcgcgtc ttggaagtct aaaacgcccg gcctgccgcg
     7261 cgtcagcatc aggttgcgga cgataaagtc gcggtgcaca tagactttgg gctgcgccaa
     7321 caggggcggc agcagcgtat cgacggtttg ctgccaaagt tggcgttgtt tgaatgttaa
     7381 ttcgcgcccc aattcttttg cgacaaacca ttccgggaac aggttgattt cgcgcaacat
     7441 cgtttcacgg tcatattcgg gcaaaacccc ttcacggctc gccttctgca attcgaccaa
     7501 ctcgccgatt gcctccaaaa gcagggcttt gtgcgccgtt tcgccctgtt cctgaagcat
     7561 tgcggtcaaa aacgtcgtat tgccaaagtc gttcaatacc acaaacccca gatccgtgtc
     7621 cgcgtgcaat acctgcggca cattgaccat gtcaaacagt ttctgcactt tcaaataagg
     7681 tgcgacactc atcttgtcgg gcggtgcatc catgcagacg acactgctgc cgtctgaaaa
     7741 cgttgcacgg aaatagcggc ggaaatcagc atccgccgcc gcaaaagtca gatcgaagtc
     7801 ccgttcggga taaacggtct gaagccaatt tttcagttcg gtttgccgtt gcataacagt
     7861 actaaagcat ttcaggttac aataaacgct attctaactg gcaaaccgac ttgaggggcg
     7921 attttggctc gtttattttc actcaaacca ctggtgctgg cattgggcct ctgcttcggc
     7981 acgcattgcg ccgccgccga tgccgttgcg gcggaggaaa cggacaatcc gaccgccgga
     8041 ggaagcgttc ggagcgtgtc cgaacccata cagcctacca gcctgagcct cggttcgacc
     8101 tgcctgtttt gcagtaacga aagcggcagc cccgagagaa ccgaagccgc cgtccgaggc
     8161 agcggcgaag catccatccc cgaagactat acgcgcattg ttgccgacaa ggtggaagga
     8221 cagtcgcagg tgcaggtgcg tgccgaaggc aacgtcgtcg tcgaacgcaa ccggacgacc
     8281 ctcaataccg attgggcgga ttacgaccag tcgggcgaca ccgttaccgc aggcgaccgg
     8341 ttcgccctcc aacaggacgg tacgctgatt cggggcgaaa ccctgaccta caatctcgag
     8401 cagcagaccg gcgaagcgca caacgtccgc atggaaatcg aacaaggcgg acggcggctg
     8461 caaagcgtca gccgcaccgc cgaaatgttg ggcgaagggc attacaaact gacggaaacc
     8521 caattcaaca cctgttccgc cggcgatgcc ggctggtatg tcaaggcagc ctctgtcgaa
     8581 gccgatcggg aaaaaggcat aggcgttgcc aaacacgccg ccttcgtgtt cggcggcgtt
     8641 ccccttttct atacgccttg ggcggacttc ccgcttgacg gcaaccgcaa aagcggcctg
     8701 cttgttccct cactgtccgc cggttcggac ggcgtttccc tttccgttcc ctattatttc
     8761 aaccttgccc ccaatctcga tgccacgttc gcgcccggcg tgatcggcga acgcggcgcg
     8821 gtctttgacg ggcaggtacg ctacctgcgg ccggattatg ccggccagtc cgacctgacc
     8881 tggctgccgc acgacaaaaa aagcggcagg aataaccgct atcaggcgaa atggcagcat
     8941 cggcacgaca tttccgacac gcttcaggcg ggtgtcgatt tcaaccaagt ctccgacagc
     9001 ggctactacc gcgactttta cggcaacaaa gaaatcgccg gcaacgtcaa cctcaaccgc
     9061 cgtgtatggc tggattatgg cggcagggcg gcgggcggca gcctgaatgc cggcctttcg
     9121 gttctgaaat accagacgct ggcaaaccaa agcggctaca aagacaaacc gtatgccctg
     9181 atgccgcgcc tttccgccga ttggcgcaaa aacaccggca gggcgcaaat cggcgtgtcc
     9241 gcacaattta cccgcttcag ccacgacagc cgccaagacg gcagccgcct cgtcgtctat
     9301 cccgacatca aatgggattt cagcaacagc tggggttacg tccgtcccaa actcggactg
     9361 cacgccacct attacagcct caaccgcttc ggcagccaag aagcccgacg cgtcagccgc
     9421 actctgccca ttgtcaacat cgacagcggc gcaacttttg agcggaatac gcggatgttc
     9481 ggcggcggag tcctgcaaac cctcgagccg cgcctgttct acaactatat tcctgccaaa
     9541 tcccaaaacg acctgcccaa tttcgattcg tcggaaagca gcttcggcta cgggcagctc
     9601 tttcgcgaaa acctctatta cggcaacgac aggattaaca ccgcaaacag cctttccgcc
     9661 gccgtgcaaa gccgtatttt ggacggcgcg acgggggaag agcgtttccg cgccggcatc
     9721 ggtcagaaat tctatttcaa ggatgatgcg gtgatgcttg acggcagcgt cggcaaaaaa
     9781 ccgcgcaacc gttccgactg ggtggcattt gcctccggca gcatcggcag ccgcttcatc
     9841 ctcgacagca gcatccacta caaccaaaac gacaaacgcg ccgagaacta cgccgtcggt
     9901 gcaagctacc gtcccgcaca gggcaaagtg ctgaacgccc gctacaaata cgggcgcaac
     9961 gaaaaaatct acctgaagtc cgacggttcc tatttttacg acaaactcag ccagctcgac
    10021 ctgtccgcac aatggccgct gacgcgcaac ctgtcggccg tcgtccgtta caactacggt
    10081 tttgaagcca aaaaaccgat agaggtgctg gcgggtgcgg aatacaaaag cagttgcggc
    10141 tgctggggcg cgggcgtgta cgcccaacgc tacgttaccg gcgaaaacac ctacaaaaac
    10201 gctgtctttt tctcacttca gttgaaagac ctcagcagtg tcggcagaaa ccccgcagac
    10261 aggatggatg tcgccgttcc cggctacatc cccgcccact ctctttccgc cggacgcaac
    10321 aaacgaccct gaccgtcgga aacccggcag gagcaccgtt cccgcacaag acggcattcc
    10381 accgacaacc ccaaacccgc catcaaaggt aggattcaaa cgataaggaa agaatgatga
    10441 aaatcaaagc cctgatgatt gccgccgcat tgctggcagc agccgatgtc cacgccgcac
    10501 cgcaaaaggc aaaaaccgca tccgccaaag ttgccaaagt tgccaaagtt gccaaagttg
    10561 ccaaagttgc caaagttgcc aaagttgcca aagttgccgc cacggcgcaa aaagaagccg
    10621 cacccgcaca acagcagggc ggtatccgct tttcagacgg cattgccgcc gttgccgaca
    10681 acgaagtcat cacgcgccgc cggcttgccg aagccgttgc cgaagccaaa gccaacctgc
    10741 ccaaagaggc gcagataagc gaatccgagc tgtcccgaca ggtgctgatg cagcttgtca
    10801 accaatccct gattgtacag gcgggcaaac gccgcaacat tcaagcaagc gaagcggaaa
    10861 tcgatgccgt cgtcgcaaaa aattccgccc tcaaaaacct cagccccgcc caacgccgcg
    10921 attttgccga caacatcatt gccgaaaaag tccgccagca ggcagtgatg cagaacagcc
    10981 gcgtgagcga agctgaaatc gatgccttcc tcgagcaggc gcaaaaacaa ggcatcaccc
    11041 tgcccgaagg cgcaccgttg cgccaatacc gcgcccaaca catcctgatt aaagccgaca
    11101 gcgaaaacgc cgccgtcggc gcggaaagca ccatccgcaa aatctacgga gaggcccgca
    11161 gcggcacaga cttttccagc ctagcgcgcc aatattcgca agacgcgagc gcgggcaacg
    11221 gcggagattt gggctggttt gccgacggcg tgatggttcc cgcctttgaa gaagccgtcc
    11281 acgcgctcaa acccggacag gtcggcgcgc ccgtccgcac ccaattcggc tggcatatca
    11341 tcaaattgaa cgaagtgcgc gatgccggca cacctcagga acgtatccgc aattccgtgc
    11401 ggcaatacat cttccaacaa aaagccgaac aggcaaccgt caacctgttg cgtgacctgc
    11461 attccggcgc gtatgtcgac atccgctaag gcggtttgaa gcaaaaagcc ataccgatcg
    11521 gcaaaaatcc gggcggtatg gctttttgga tttcgagtta cttttacacc gtcattcatc
    11581 attcccgcga aagcgggaat ctagaaacga aaagtaacag gaatttatcg ggaatggctg
    11641 gagtttaaag gactggattc ccgccgtcgc gggaatgacg ggattttggg ttgtggtaat
    11701 ttatcggaaa aacaaaaaaa cctatgccgt cattcccgag caggcgggaa tccggttatt
    11761 taaaactgca gaaatttatc cgaagcaaca acaatctttc catcgtcatt cccgcg
//
LOCUS       DAXHPG010000050        11744 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXHPG010000050 DAXHPG010000000
VERSION     DAXHPG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.050"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(354..776)
                     /locus_tag="ACFM6F_001004"
     CDS             complement(354..776)
                     /locus_tag="ACFM6F_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC6164441.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(894..1565)
                     /locus_tag="ACFM6F_001005"
     CDS             complement(894..1565)
                     /locus_tag="ACFM6F_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC6164442.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1581..2207)
                     /gene="purN"
                     /locus_tag="ACFM6F_001006"
     CDS             complement(1581..2207)
                     /gene="purN"
                     /locus_tag="ACFM6F_001006"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC6164443.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSVLPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2314..3132)
                     /locus_tag="ACFM6F_001007"
     CDS             complement(2314..3132)
                     /locus_tag="ACFM6F_001007"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6164444.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3391..3831)
                     /locus_tag="ACFM6F_001008"
     CDS             complement(3391..3831)
                     /locus_tag="ACFM6F_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC6164445.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3894..5300)
                     /locus_tag="ACFM6F_001009"
     CDS             complement(3894..5300)
                     /locus_tag="ACFM6F_001009"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC6164446.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5470..6585
                     /gene="lptF"
                     /locus_tag="ACFM6F_001010"
     CDS             5470..6585
                     /gene="lptF"
                     /locus_tag="ACFM6F_001010"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC6164447.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6582..7652
                     /gene="lptG"
                     /locus_tag="ACFM6F_001011"
     CDS             6582..7652
                     /gene="lptG"
                     /locus_tag="ACFM6F_001011"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC6164448.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7952..10537
                     /gene="acnB"
                     /locus_tag="ACFM6F_001012"
     CDS             7952..10537
                     /gene="acnB"
                     /locus_tag="ACFM6F_001012"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC6164449.1"
                     /translation="MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10679..11674
                     /locus_tag="ACFM6F_001013"
     CDS             10679..11674
                     /locus_tag="ACFM6F_001013"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC6164450.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         2692 a         3264 c         3056 g         2732 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttttgt taatccacta taattcaata aattaatata
       61 tggcttaaaa taacgggatg cccgcctccc gtcctcctgc agaagcgggc gggtatcatt
      121 ttaaaacgcg gcatttaaaa tttgaccgaa aattgttgac aatccggaat caagtctgca
      181 caataccccg acaagtccaa gtattataaa ggctgaataa agaggaaaca gcaggcagat
      241 atattcggga ggtgcagtcc gaatatatct gcttttttat gcgcctccgg attgcctgcc
      301 gcacctttcc cttcagacgg catcagccgt ttccccataa tgccgcccga tgcctattta
      361 tctgccccgg caatttcaaa actgtgggta atctttgccg ctttgcccaa cataatcgaa
      421 gccgaacagt atttttcggc agacatctga acggcgcgct caatggccga ttctttcaaa
      481 tcatgcccga atactttgaa atggatgtgg atttcggtaa acacgcgcgg cgcatcgtcc
      541 gcccgtttcg ccgtaaccgt cgcacggcag tcagtcactt tctgacgctg tttttcggca
      601 atcatcacca catcgatgct cgaacagccc gccacgccca acagcagcat ttccaaaggg
      661 ctgggcccgc gcttagcctt accttctgcc gccgacccct ccataacgac gctgtgcccg
      721 ccttccgttg ttccgacaaa gcacattccg tctatccatt ttgatgtaac ctgcatggtg
      781 tcattcctga aaatagcgtt aaaaccgctt tgcatatggc gttattgtaa acaatttcaa
      841 gcggcttatg cagaaatatg gacaaaacgg caaaaaaacg cctgaaaatc gatttacggt
      901 ttggcggact ggccattgat ctgcaccgat ttgagtttca gcgtataggt tttgccgtcg
      961 tcggtatagc cgatttgtgc cggaatattg ttcagggacg gtgcgaagaa atacattacc
     1021 gtatcgtcgc cgcgccgcac ccgatatttg acgacttcgg tttccacgcc gcctatgctg
     1081 tattttcctg tacccgcctt attcaaaccg ccgacggaat aaagtttttt gccgttggtg
     1141 attttcagcc ccggggggag tttcgcgtca tttgccgcca actgccaggc aagcgtgaac
     1201 aaatccatag ccttggggct ttgctcggtt ttgctctcgc ccgctttgcc gtaggttacg
     1261 ctgccgtcgg cgaatttggc ttccgcatac agtttgcccc tgcgtatgtc tctatagtag
     1321 gtagggtgca gggtattgcc gacaaccgta ccgccggact cgaaacggat attgtatagc
     1381 ggcactttaa tcgtcgaaac gattttgtaa gcattgccgc tgcgttcaaa tgtcatcgtg
     1441 gcgggaatgc cgtagctgcc ggaatagtgc agcacggcgg attggggcag ccctgccgca
     1501 tacgcgcacg gcagggcggc ggacaaaatg gcggcggaaa atatattttt aaaagtcttc
     1561 atcatttgct cccgcccggt ttacgccgtc aggaaacggg cggcatcggc gttttccgaa
     1621 tttctgacgc ggtttccctc gataatcagg cggccggcgg caaaatcggc aacggctttc
     1681 ggatagagtt tgtgttcgac agccaaaacc cgggcggcaa catcgtctgc cgtatcgccg
     1741 tcgagtatcg gcacaatccc ttgcgataca atcgggccgc aatccagttc ggcagtaacg
     1801 aaatggatgg tgcagccggc aacgcggcag cccgcctcca aagcgcgttc gtgcgtatga
     1861 agtccggtaa acgagggcag gacggacggg tgaatgttca tcagcctgcc ttcgtaatgg
     1921 gcgcaaaact caggggtcag aatccgcata aaacctgcca aaaccaccaa gtcgggttga
     1981 tatgcgtcga ttttttccat catggcggta tcgaaggcaa gtcgtgattc aaagttttta
     2041 tgattcaggc tgtcggtcgg aataccctga ccggccgccc attgcaaacc ggcagccgtt
     2101 tcgctgttgc tcaacacggc ggcaatacgg acgttgggaa tggcggcatt gacgattgcc
     2161 tgcatattgc tgccgcgtcc ggaaatcagg atgacgatgt ttttcataat ggtgcgcttt
     2221 tgaaagggat gccatctgaa ccgctgtttg gtggtttcag acggcatttg ccgtaaaaat
     2281 gcccgaaaac ctgtttcggg catggattcg gacttaattt acttttttga tgtcgacttg
     2341 agccggctgc ttggcgggcg cgttttcggg tgcgccgatt ttgaccagtt tcacatcaaa
     2401 taccaaagtg gcgttcggac cgattttgtc gcccgcaccc tgttcgcggt aggcaaggtt
     2461 ggacgggatg tagaacgtgg cttcgccgcc ttctttcaga agctgtacgc cttcggtcca
     2521 acccggaatc acttggctca aagggaaggt gaccgggccg ccgttggctt tgctgctgtc
     2581 gaataccgta ccgtcaatca ggcggccttc gtattccacg gtaacgatgt cgtctttggt
     2641 cggctgtttg ccttcaccct gtttggtgat tttgtattgc aggccggaag cagtggtctt
     2701 cacgccgtct ttggcggcat tttctttcag aaaggcttcg cctttttctt tattggcctt
     2761 cgcgtccgcc ttgtgttttt ctacggcttt agcctgttgt tcctgaagga atttcatcat
     2821 gacttcctga gcctgctctt cggtcatttt gatttctttg ccgtcataca ctgcctgcat
     2881 ggcttcggta aagactttca aatcgatttc cgcgccctgt tccttcattt gcttcaggga
     2941 gcgtccgatg tccacgccca tcgcatagct tgcctgctgc atcgtgctgc cgatcgaaga
     3001 ggtgtcgccc tgcgcggaag aagcggcggc aggttcggat gcagatgcgg gggcggcttc
     3061 ttttttgccg caggcggaaa gtgccaaagc ggcggaaagg gtcagtgcgc tgattttgaa
     3121 aatggtgttc atgatggatc ttcgctgtcg ataaggtcgg aaaaacggga ttatagccga
     3181 gtttgaatgt ttcaacacac aggatgacac ataaagcgtc aatcgtgtgt tgccctgttt
     3241 tggaaaggat tgaaccttcc aaaataagtt ttgattctac cgccccgagg gacagatgtc
     3301 caagtggcgg ggttcaaccg ataaggaaat tttaatcaaa tagaatcaag cctgtttaaa
     3361 ttttgtaaat gcggcatttc agacggcatt ttatgccttg ccctccatgc cgtgatgttc
     3421 gatggcaaaa ccgcttcggc ggtaggcggt aaagcgttcg cgcgcgtcgg cgagatcttc
     3481 caagctgttg ccgacgattt ccaaaacgcg cgcgggcaag accggagcgg tgttccaaaa
     3541 accgtcggac aggttcaaaa cagtcatgcc ttcgggaatt cggggcggat tgccgccgca
     3601 agcaagcagg acggatgttt cagacggcat ggcttcttcc atttcccaaa tttcgtgcgg
     3661 aataaaactc tcggcctcgt attgccaaag cattttgtcc aattcctgaa gctgcccgaa
     3721 cgaatcggac cacaccagta tcctgccgcc gtcccgaatc gcacgggcaa tcaggcggca
     3781 ggtgaaaatc ggaacttggg caacgtgcgt gtaaaaggtg gctttcggca tattgtttga
     3841 gcatttggca ggacgatgcc gtctgaaagg cttcagacgg cattgtggca aaattaaaga
     3901 ttccgcagat agttcagcag caagggaacg ggacggccag tcgcaccttt ttccgctccg
     3961 gatttccacg ccgtacccgc gatgtcaagg tgcgcccacg ggtagccttc ggtgaagtaa
     4021 gacaggaatg ttgcggcggt aatcgtgccc gcgccgggcg tgccgatgtt tggaatgtcg
     4081 gcaaagttgg atttgagttg gtctttgtag gtctcaaaga gcggcagttg ccatgctttg
     4141 tcgtccacgt tgtaggaagc ggcaagcagg ctgtcgatca aatcctgatt gttgcccatc
     4201 acgccgctga catcgtgccc caaggcaaca atacacgcgc cggtcagggt ggcgacgtcg
     4261 atgacggctt tgggtttgaa ctgctcggcg taagtgagcg cgtcgcacaa aatcagacgg
     4321 ccttcggcat cggtgttcaa cacttcgatg gtcagacctt tcatactttt cacgacatcg
     4381 cccggtttgt ttgccgcgcc ggaaggcata ttttcacaag tggcgacgac ggcaatcagg
     4441 ttaatcggca gttgcagttt gacggcggcg cagaaggtgc tgatgacggt tgccgcgccg
     4501 cacatatcaa acttcatttc gtccatgttc aggccgggct tgagggagat gccgccggtg
     4561 tcgaaggtaa tgcctttgcc gaccaatacc acaggcgcgg cttctttgtc ggctgcaccg
     4621 aaatagctca gttcgaccaa atagggggct tccgcgctgc ctttggcgac cgaccaaaac
     4681 gaacccatgt tttctttgat gtagtctttt tcgatgattt tggcgtgcgc gcccagtttt
     4741 tcggcttcgg ctttggcggt gcgcgctaaa aattcgggcg tgcattcgtt gggcgcggcg
     4801 ttgcccaagt cgcggcagag gctttgtccg taaacttgcg cttcggcgac gcgcaaggct
     4861 tctttaacga cggcttcgtg tgcgctgtgg aacacggcgg tttcaaattt ggcgggcttg
     4921 gcttcttttt tgtagcggtc gaaacggtag gcggcgttgc cgaacgcaat cgcaaacgct
     4981 tcggctacgg cggcggcttg cgcttcatcg aaggcatgga tgtccacatt gaccgtttcc
     5041 tgattttgcg cccatttggc ggcttcggcg gcggccttgt tcaatgcggc gcggccggtg
     5101 cttttcagac agcatacggc aacagcctgc aaaccgttgc ctgtcgggat ttttgtgtcg
     5161 gcaaaatttt gaccttcttc aagcgaagac aaaagggcaa gggcggtcgg atggtttaat
     5221 tgcgcctctt cggtgcagac aaataactgt gcgcctgcct gctgttcctg caagatttcg
     5281 gtttttgtgc taaattccac gtttattctc ctgattgaga cggttgtcgg tagttttcgg
     5341 acggcctttc gctcaaaaga ccgtctgaag acggctggca cgattgtacc ccatttgaag
     5401 caccgtctga aaccttgcgc ggacaatccg cctgcgccga accgcttacc gccccctgac
     5461 cgcgattcta tgatttatca aagaaacctc atcaaagaac tctcttttac cgccgtcggc
     5521 attttcgtcg tcctcttggc ggtattggtc tccacgcagg caatcaacct gctcggccgt
     5581 gccgccgacg ggcgtgtcgc catcgatgcc gtgttggcat tggtcggctt ctgggtcatc
     5641 ggtatgacgc cgcttttgct ggtgttgacc gcatttatca gtacgttgac cgtgttgacc
     5701 cgctactggc gcgacagcga aatgtcggtc tggctatcct gcggattggc attgaaacaa
     5761 tggatacgcc cggtgatgca gtttgccgtg ccgtttgccg ttttggttgc cgtcatgcag
     5821 ctttgggtga taccgtgggc agagctacgc agccgcgaat acgctgaaat cctgaagcag
     5881 aagcaggaat tgtctttggt ggaggcaggc gggttcaaca gtttgggcaa gcgcaacggc
     5941 agggtttatt ttgtcgaaac cttcgatacc gaatccggca tcatgaaaaa cctgttcctg
     6001 cgcgaacagg acaaaaacgg cggcgacaac atcatcttcg ccaaagaagg taacttctcg
     6061 ctgaacgaca acaaacgcac gctcgaattg cgccacggct accgttacag cggcacgccc
     6121 ggacgcgccg actacaatca ggtttccttc caaaaactca acctgattat cagcaccacg
     6181 ccaaaactca tcgaccccgt ttcccaccgc cgtaccattc cgaccgccca actgattggc
     6241 agcagcaacc cgcaacatca ggcggaattg atgtggcgca tctcgctgac cgtcagcgtc
     6301 ctcctactct gcctgcttgc cgtgccgctt tcctatttca acccgcgcag cggacatacc
     6361 tacaatatct tgattgccat cggtttgttt ttaatttacc aaaacgggct gaccctgctt
     6421 tttgaagccg tggaagacgg caaaatccat ttttggctcg gactgctgcc tatgcacatc
     6481 atcatgttcg tcatcgcaat cgtacttctg cgcgtccgca gcatgcccag ccagcccttc
     6541 tggcaggcgg ttggcaaaag tctgacattg aaaggcggaa aatgaacctg atttcacgtt
     6601 acatcatccg tcaaatggcg gttatggcgg tttacgcgct ccttgccttc ctcgctttgt
     6661 acagcttttt tgaaatcctg tacgaaaccg gcaacctcgg caaaggcagt tacggcatat
     6721 gggaaatgct gggctacacc gccctcaaaa tgcccgcccg cgcctacgaa ctgattcccc
     6781 tcgccgtcct tatcggcgga ctggtctccc tcagccagct tgccgccggc agcgaactga
     6841 ccgtcatcaa agccagcggc atgagcacca aaaagctgct gttgattctg tcgcagttcg
     6901 gttttatttt tgctattgcc accgtcgcgc tcggcgaatg ggttgcgccc acactgagcc
     6961 aaaaagccga aaacatcaaa gccgccgcca tcaacggcaa aatcagcacc ggcaataccg
     7021 gcctttggct gaaagaaaaa aacagcatta tcaatgtgcg cgaaatgttg cccgaccata
     7081 cgcttttggg catcaaaatt tgggcgcgca acgataaaaa cgaattggca gaggcagtgg
     7141 aagccgattc cgccgttttg aacagcgacg gcagttggca gttgaaaaac atccgccgca
     7201 gcacgcttgg cgaagacaaa gtcgaggtct ctattgcggc tgaagaaaac tggccgattt
     7261 ccgtcaaacg caacctgatg gacgtattgc tcgtcaaacc cgaccaaatg tccgtcggcg
     7321 aactgaccac ctacatccgc cacctccaaa acaacagcca aaacacccga atctacgcca
     7381 tcgcatggtg gcgcaaattg gtttaccccg ccgcagcctg ggtgatggcg ctcgtcgcct
     7441 ttgcctttac cccgcaaacc acccgccacg gcaatatggg cttaaaactc ttcggcggca
     7501 tctgtctcgg attgctgttc caccttgccg gacggctctt cgggtttacc agccaactct
     7561 acggcatccc gcccttcctc gccggcgcac tacctaccat agccttcgcc ttgctcgccg
     7621 tttggctgat acgcaaacag gaaaaacgtt gaaccaatgc cgtctgcccc ccttcggacg
     7681 gcattggttt gtattatcat attcccgtag acagatagcc gttccctatt atagtggatt
     7741 aacaaaaacc agtacggcgt tgcctcgcct tgccgtacta ttgtactgtc tgcggcttcg
     7801 tcgccttgtc ctgatttaaa atacattacc tgtcataaca gttccatttt tgttaaaact
     7861 agtctatgat agcggtacaa atattgttta caatatttaa cgcaaatcat ttgcaacccg
     7921 acaaaagaaa aacagaaaaa ggaacaaaga gatgttagaa gcctatcgta aagccgccgc
     7981 cgagcgcgcc gcccttggta ttcccgccct tcctctgact gctcagcaaa ccgccgattt
     8041 ggttgagctg ctgaaaagcc cgcccgcagg cgaaggcgag ttcttggtcg aactgcttgc
     8101 ccaccgtgtt ccgcccggtg tggacgatgc cgccaaagtc aaagcctcat tcctggctgc
     8161 cgttgccgaa ggcagcgcgt ccagcccgct gatctccccc gaatatgcga ccgaactctt
     8221 aggtacgatg ctcggcggtt acaatatcca cgccttaatc gaactcttgg acgacgacaa
     8281 actcgcgccc attgccgcca aaggcttgaa acatacgctt ctgatgttcg attccttcca
     8341 cgacgttcag gaaaaagccg aaaaaggcaa caaatacgcg caagaagttt tgcaatcttg
     8401 ggcagatgcc gaatggttcg cctcacgcgc caaagttccc gaaaaaatca ccgttaccgt
     8461 tttcaaagtt gacggcgaaa ccaatacaga cgacctctcc cccgcgcccg acgcgtggag
     8521 tcgtcccgat attccgctgc acgcgctggc catgctgaaa aacccgcgcg acggcatcac
     8581 gcccgacaaa ccgggcgaag tcggtccgat taaattgttg gaagaactca aagccaaagg
     8641 ccatccggtt gcttacgtcg gcgacgtggt cggtactggt tcttcacgca aatccgcgac
     8701 caactccgtc atttggcata ccggcgaaga cattccgttc gtgccgaaca aacgcttcgg
     8761 cggcgtatgt ttgggcggca aaatcgcgcc gattttcttc aatacccaag aagattccgg
     8821 cgcgctgccg attgaagtcg atgtatctgc tctaaaaatg ggcgatgtcg tcgatatcct
     8881 gccttatgaa ggcaaaatcg tgaaaaacgg cgagactgtt gccgagtttg aattgaaatc
     8941 acaagtattg ctggacgaag tgcaagccgg cggccgtatc aacctgatta tcggtcgagg
     9001 tctgaccgcc aaagcgcgcg aagccctgaa actgcctgcc tctactgcat tccgcctgcc
     9061 gcaagcgcct gccgaaagca aagccggttt caccttggcg caaaaaatgg tcggccgcgc
     9121 ctgcggtctg cccgaaggac aaggcgtgcg cccgggtact tactgcgaac cgcgtatgac
     9181 gacggtcggc tcgcaagaca cgaccggccc gatgacccgc gacgagttga aagacttggc
     9241 ttgtttgggc ttctccgccg atatggtgat gcagtctttc tgccacaccg ccgcctatcc
     9301 gaaacctgtc gatgtaaaaa cccataaaga actgcccgcc tttatttcca cccgtggcgg
     9361 cgtgtcactg cgtccgggcg acggcgtcat ccactcgtgg ctcaaccgcc tgctgctgcc
     9421 cgataccgtc ggcaccggcg gcgacagcca tacccgtttc cccatcggta tttccttccc
     9481 cgccggctcc ggcttggttg cctttgccgc cgcaacgggc gtaatgccgc tcgatatgcc
     9541 cgagtctgta ttggtacgct tcagcggcaa gctgcaaccg ggcgtaaccc tgcgcgattt
     9601 ggtgaacgcc atcccgctgt acgcaatcaa acaaggtttg ctgaccgttg ccaaagccgg
     9661 taagaaaaac atcttctccg gccgcatcct cgaaatcgaa ggcctgcctg atttgaaagt
     9721 ggaacaagcc tttgaattga ccgacgcatc cgccgaacgc tccgccgccg gctgtaccgt
     9781 gaagctcaac aaagagccga ttatcgagta catgaaatcc aacgtcgtgt tgatgaaaaa
     9841 catgattgcc aacggctatc aagacccgcg cactttggaa cgccgcatca aagctatgga
     9901 aaaatggctg gcaaatcccg agttgctcga agcggataaa gatgccgaat acgccgccgt
     9961 gattgaaatc aacatggacg acatcaaaga gccgattatc gcctgcccga acgacccgga
    10021 cgacgtgtgc ttcatgtccg aacgctccgg caccaaaatc gacgaagtat tcatcggttc
    10081 gtgtatgacc aacatcggcc acttccgcgc cgcctccaaa cttttggaag gcaaggcaga
    10141 cacccccgtc cgcctgtgga ttgcgccgcc gaccaaaatg gacgcgaaac aattgtccga
    10201 cgaaggacac tacggcgtac tcggacgtgc cggcgcgcgt atggaaatgc cgggttgctc
    10261 cttatgtatg ggtaatcagg cgcaagtacg cgaaggtgcg accgttatgt ccacctccac
    10321 ccgcaacttc ccgaaccgtt tgggtaaaaa cacctttgtt tacctcggtt cggcggaatt
    10381 ggcagcgatt tgctccaaac tgggtaaaat cccgaccgtt gaagaatatc aagccaatat
    10441 cggcatcatc aacgaacagg gcgataaaat ctaccgctat atgaacttca acgaaatcga
    10501 cagctacaac gaagtagccg agaccgtgaa cgtttaatcc ccgtcatccg tatgaagtaa
    10561 gggattgacc gcaatgccgt ctgaacaacc ttcagacggc attgcaacat tccgctaacc
    10621 cttctttccg caaacgctgc aaatacggcg ttcacgcccc cacataaagg aaacgacagt
    10681 gaacctgaaa aaccgccatt ttctgaaact tttagacttc acgccggaag aaatcaccgc
    10741 ctacctcgac cttgccgccg aattgaaagc cgccaaaaaa gcagggcgcg agattcagcg
    10801 gatgaaaggg aaaaacatcg ccctgatttt tgaaaaaacc tctactcgga cgcgctgcgc
    10861 gtttgaagtc gccgcgcgcg atcaaggcgc gggagtgact tatttagagc cgtccgccag
    10921 ccaaatcggg cataaggaaa gcatcaaaga caccgcccgc gtgttgggca ggatgtacga
    10981 tgccatcgaa tatcgcggtt tcggtcagga agttgttgaa gaattggcga aatacgcggg
    11041 cgtacccgtg ttcaacgggc tgaccaacga gttccatccc acacaaatgc ttgccgacgc
    11101 actgactatg cgcgaacaca gcggcaaacc tttgaaccaa accgcgtttg cctacgtcgg
    11161 cgacgcgcgt tacaacatgg gcaattccct gctgatttta ggggcaaaat tggggatgga
    11221 cgtgcgtatc ggcgcaccgc aaagcctgtg gccgtctgaa ggcattattg ccgccgcaca
    11281 cgccgccgcc aaagaaaccg gcgcaaaaat taccctgacc gaaaacgcgc atgaagccgt
    11341 gaagaatgtt gattttattc ataccgatgt gtgggtcagc atgggcgagc cgaaagaagt
    11401 ctggcaggaa cgcatcgatt tgctgaaaga ttaccgcgtt acgcccgaac tgatggcggc
    11461 atcgggcaat ccgcaagtca aattcatgca ctgcctgccc gccttccaca accgcgaaac
    11521 caaagtcggc gaatggattt acgaaacctt cgggctgaac ggtgtggaag ttacagaaga
    11581 agtattcgaa agccccgcca gcatcgtgtt cgatcaggcg gaaaaccgta tgcacacgat
    11641 taaagcggta atggtcgcgg ctttgggcga ctgacagaac tgtgcctgtt taaattcatc
    11701 cgcaacacag ataccgtctg aacacgatgt tcagacggta tcca
//
LOCUS       DAXHPG010000051        11426 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXHPG010000051 DAXHPG010000000
VERSION     DAXHPG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.051"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..186
                     /locus_tag="ACFM6F_001014"
     CDS             <1..186
                     /locus_tag="ACFM6F_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6164451.1"
                     /translation="NRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAM
                     CLNLLKAANRLSAPAAA"
     gene            complement(439..651)
                     /locus_tag="ACFM6F_001015"
     CDS             complement(439..651)
                     /locus_tag="ACFM6F_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC6164452.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            complement(663..944)
                     /locus_tag="ACFM6F_001016"
     CDS             complement(663..944)
                     /locus_tag="ACFM6F_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164453.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(1273..1845)
                     /locus_tag="ACFM6F_001017"
     CDS             complement(1273..1845)
                     /locus_tag="ACFM6F_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFC6164454.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            complement(1820..2164)
                     /locus_tag="ACFM6F_001018"
                     /pseudo
     CDS             complement(1820..2164)
                     /locus_tag="ACFM6F_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2157..3032)
                     /locus_tag="ACFM6F_001019"
     CDS             complement(2157..3032)
                     /locus_tag="ACFM6F_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HFC6164455.1"
                     /translation="MSNEVEVMDAVSVQNYQVAHSVMVMEQWGNGEVYNEATWIERGR
                     LAVRQTMEGMFELGRALIVLKEHTEHGRFMEIVKGQFGIGHNETARLMSATQRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(3078..3368)
                     /locus_tag="ACFM6F_001020"
     CDS             complement(3078..3368)
                     /locus_tag="ACFM6F_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6164456.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRRQHERLQSKIAEIGKRVNAD"
     gene            complement(3380..3625)
                     /locus_tag="ACFM6F_001021"
     CDS             complement(3380..3625)
                     /locus_tag="ACFM6F_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6164457.1"
                     /translation="MINHECINQPYGRILKFSRVQAKEMLTADELKENFAQNGQTLAQ
                     WARENGFKPRDVYLVIGGQRKGNYGKGYEIAKKLGLK"
     gene            complement(3618..3842)
                     /locus_tag="ACFM6F_001022"
     CDS             complement(3618..3842)
                     /locus_tag="ACFM6F_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6164458.1"
                     /translation="MEKPLNFKPIPYPQTRESAAGWFKRNGVCKAHWAKYFNLERTTV
                     EHLLRGKLKGNFGKSHEAAVLLGMKEKSDD"
     gene            4003..4485
                     /locus_tag="ACFM6F_001023"
     CDS             4003..4485
                     /locus_tag="ACFM6F_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6164459.1"
                     /translation="MSFQSVHFFVLSRDDLGCLCFDVEIIGRILPILQGIFMNNSSFF
                     GNRLKEERKKLKMTQAEIAEKCGVSGRMWGDYERGISQPKAELFFQFEKVGIDVQYVM
                     HGRRGETAVMPSETLNAEEQELLALFREAAAADREMILMVARRAEKKAQTALGKVSNG
                     "
     gene            4478..5110
                     /locus_tag="ACFM6F_001024"
     CDS             4478..5110
                     /locus_tag="ACFM6F_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164460.1"
                     /translation="MDKMNQFELCQKEHVNPFALSKQYLLVVTFVKSSSKNFQAALLW
                     ARSAKLFENLEIGKETIYCCAFDKTAEQAGMAGVFLNYIENWNGKQIYINGRIHSGSI
                     YDLLGVLDCYQKSQSCPNPKSHCCFVSDDIFLWHGSRPTFEISLDLTGKKKETSSAKK
                     FVMPCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENFRQYE"
     gene            5103..5297
                     /locus_tag="ACFM6F_001025"
     CDS             5103..5297
                     /locus_tag="ACFM6F_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6164461.1"
                     /translation="MNNYYLYRNCSSDVLWVKRIQRQIDGSLLLISDNSTYPPMPLAL
                     AEHPDIQIIGQVVQVSKDLN"
     gene            5321..5881
                     /locus_tag="ACFM6F_001026"
     CDS             5321..5881
                     /locus_tag="ACFM6F_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164462.1"
                     /translation="MKILALLIAATCALSACGGQAEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSVANKGLADQKTGLTLPEHVVPADNAEGKNLLHDFSDGLTLTVDTDKADKITA
                     VRVVWNTDAMPQKAEKLSKAAAALIAATAPGDRTLLRDTGDQIKMAIDSHNAQKEPTR
                     EWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            6022..7080
                     /locus_tag="ACFM6F_001027"
     CDS             6022..7080
                     /locus_tag="ACFM6F_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164463.1"
                     /translation="MKNKLTLRYFKITRCGYWSGSSKEPNHYEDLLKTLTHLETYVKG
                     KTIEETGISRDDKEPSTFLLDICKKNGLWLLAFWNPAAESSSDEIVSLNMKSKLGQVD
                     SSPVTAGNGKTFGFVSYFLIWPEHNFYSTILSPNATLAGRTEFEEYMLNFLWIDPNFI
                     EKTTYEETSTDGIIHTMERTNFKLPLKINGTPVPRFAGKLAKSPSEKEYVISNYDQVT
                     QVHNQCTVKVTSGQSSSGILKKMLPSFFGSTELFAPLKTIRVNHSVSAAFENKSNLKD
                     WIEDWEKNSFDLDKDDCGFKLKNDDKIYWIRGNVIKCELDVNLHKENGIYPAAKILDI
                     IDQRKDYVLNEFKLSDDS"
     gene            7109..7576
                     /locus_tag="ACFM6F_001028"
     CDS             7109..7576
                     /locus_tag="ACFM6F_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164464.1"
                     /translation="MIGVAWYLGANIRFEEQWVLYEALRTTASIIFAVVGVWLAIVYP
                     ERLKSPFSQTFSSPVYRRGFKQLFSPVISSIFILSIVLLVGLLAPLLKHIEAIIQYIP
                     LCRKISFSLLASLTMWQIYTVLSVLVPLLNILSKNENDCHASNIINKLKGEQN"
     gene            7680..8183
                     /locus_tag="ACFM6F_001029"
     CDS             7680..8183
                     /locus_tag="ACFM6F_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFC6164465.1"
                     /translation="MSTKFNQFINRVLSHEGGYVSHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAVRMLQRAAG
                     VADDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLSTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            8197..8370
                     /locus_tag="ACFM6F_001030"
     CDS             8197..8370
                     /locus_tag="ACFM6F_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164466.1"
                     /translation="MSKKSLIALMTAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKKRHK"
     gene            8295..8627
                     /locus_tag="ACFM6F_001031"
                     /pseudo
     CDS             8295..8627
                     /locus_tag="ACFM6F_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8624..9034
                     /locus_tag="ACFM6F_001032"
     CDS             8624..9034
                     /locus_tag="ACFM6F_001032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HFC6164467.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAKQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMAALKKLDALIYGKEVRQKERNSSQG"
     gene            9039..9587
                     /locus_tag="ACFM6F_001033"
                     /pseudo
     CDS             9039..9587
                     /locus_tag="ACFM6F_001033"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9603..9788
                     /locus_tag="ACFM6F_001034"
                     /pseudo
     CDS             9603..9788
                     /locus_tag="ACFM6F_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(10107..10469)
                     /gene="ssrA"
                     /locus_tag="ACFM6F_001035"
     tmRNA           complement(10107..10469)
                     /gene="ssrA"
                     /locus_tag="ACFM6F_001035"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(10532..11137)
                     /locus_tag="ACFM6F_001036"
     CDS             complement(10532..11137)
                     /locus_tag="ACFM6F_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC6164468.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKPPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            complement(11181..>11426)
                     /locus_tag="ACFM6F_001037"
     CDS             complement(11181..>11426)
                     /locus_tag="ACFM6F_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164469.1"
                     /translation="SDFRVSRFRQFKIGKTRQPASQGRFLKLYQAFGHIGSTGPHFRQ
                     STLPTVAAFLFDIQSHFQKTYAGNKIILPRLANIPAN"
BASE COUNT         2830 a         2590 c         2860 g         3146 t
ORIGIN      
        1 aaccgatatt tgtcgaagac ccgttatgtg gtcgaacaga gcttcggtac gctgcaccgt
       61 aaattccgct atgcgcgggc agcctatttc ggactgatta aagtgagtgc gcaaagccac
      121 ctgaaggcga tgtgtttgaa cctgttgaaa gcggccaaca ggctaagtgc gcccgctgcc
      181 gcctaaaagg cagcccggat gcctgattat cgggtatccg ggcaggatta aggggatatt
      241 tgggtagaat taggcggtat ttggggcgaa cctgtgtttg ggtttcggct gtcgggaggg
      301 aaaggaattt tgcaaaggtc tcagagtgag tttattttgg ggcggcggcg ggtcgggggc
      361 aagcggcgtg gggcttttag gtttgggggt gggtaaaaaa tgccgtaccc ggcgggcagg
      421 cttcgcactc tgaaggtttc agacggcgtt tgttttttct atccaatcga ggaactgccg
      481 ccatttttcc agcggcatat cggcccgacg ggtttcggta tcgggttcgg cttcccagcg
      541 gcctacctgt atgtagtgct tcaccccgac gatttgcgcc aactcggcct gtgtcagttt
      601 gcagcggttg cgaagggtgc gaaggttgta aggcgtgtag ccgagttcca tgtcgttgcg
      661 gtttattttg tttcgcatat ttttttgact gccctgcggc aggtttcggt aaggacggcg
      721 gcaaatcggg cttcgtctgc cggtttgccg tcgaaaaaaa taaaatcgta aagggtgatg
      781 ccgttttcgg aatggcggta atgcccgccc gggcattcgc cgtctgtctg cctgtggatt
      841 ttggcaatca ggcggggata gcggcaatgg gtaatgaaac ggcttgcgcc gtcttgcccg
      901 ataatccatt cggggtagcg gttgaatagt gcggctctgt tcattttgtt cgggttgttc
      961 gggggataaa gcccctcgcg gggcttgtgg tcaggcaaat ttgaatatca gtgccaacac
     1021 ggcggcgatg gcggtaacca gcccggtggc cgccatcatg ggataccatc gggattcttg
     1081 ggctatttta cggattcggc gttgattttg tgcgcgtctg caatgatttt ggcgatttcg
     1141 gcatctactt ttctcagact ggagtgtttc atttcttgtt cgattatgtt catcgtactt
     1201 cctttcgttt ttggcggttg ccgccgcttg tcggatggta ggatgtctgc catgtgtata
     1261 tattgatacc ttttaggttt tattgcaagt gttttgggcg gcggcttcgt atgcttggcg
     1321 gtggcggcgg ctgtaccatt cggcgagttc gcgccgctct tggaggcggt agctgtcggc
     1381 ggtcaggtag tggtgcaggc ttgagaagcc ggcgcgttgg agggcggcgc ggctgatgtg
     1441 gccgagggcg aggtctgttg tgagggtgtc ttttccggcg gtcaggctga tgttggtgaa
     1501 gaggtggcgg aatccgtgca ttgtgtgttt ggatttgccg ggggtgctgc cgtcatagcc
     1561 cagtcgtcgg atggcgttgt gggcgaattt gatgctgatg tggtcgggat ggggggcggg
     1621 tttgcgccgt gggcggatgc cggggaaaag gtggatgttg tcgccggtct gtgtgtgcag
     1681 ctctcggagt atttctaccg cccagtccga cagtgggacg gtaaaggggt gtttggtctt
     1741 catgtcggcg gcggggatgt gccataaccg ggcggtgagg tcgatgtctt gccagcgggc
     1801 ggaaagcagt gcggagggac gggatgactt ccccggtttc cccatcaatc cgcgccgcct
     1861 cgatttttcc gttggcgcgc agcatttcca ccgcctgccg caccgacatc aaccgcttgc
     1921 cgttgcgcct catcgcctcc accaaaaccg ccgaaatcaa tttggcttct tccggtttaa
     1981 gctccgtctt gcccgcatcg ctgcgccgtt tgcgcgtcgg cttgacgctg accgcctcca
     2041 gcttgcggta tagcgtggca aggctgatgc ccaattcctg cgcctgctgc ttaagatatg
     2101 cagagcgtgc gccgcgttcc attgcttccg cctgattctc gactgcctta agacgctcaa
     2161 tcattgccgg attcatcgcc ttctcccgtt tcaccgccca accattccgg cacattgtct
     2221 gtcggtgctt cagtcggcag ggcatagctt tcgcgcagtt gctcgcagtc caaaataatt
     2281 tgattgagcg tgccgaccat ctttgcctga tggctgatct cgtgtgcctc actgtgcgca
     2341 ttaagttggt cgaacaaatc tttcaaacgg ctcacttgac tgcggatacc gacctcaagg
     2401 cttgttaact gcatcgccaa ctcactgccc acgtcttccg ccttcggctc tctgacaacg
     2461 gtttgcttct tagccagctt ctcggtcagc tcatcaattt tggctgtttt ggttttcatc
     2521 acttcgtctt tggcggcgag gttttcacgg ctttcgcgca gggcgacgcg cagctcgcgc
     2581 accgtcattc ggtccacatc gtcaaaggtc atgccgttga cttcttctcc ttcggcaagt
     2641 cccaccaacg taacgtcttc ttcgaccaag agttccagca gctttgactt gcccaaatcc
     2701 atcagcttcg gcgcggcttt ctgcatttgc ggcgtggcga aacgttgggt agcagacatc
     2761 aatcgggcgg tttcgttatg gccgatacca aattgacctt ttacaatttc cataaatcgc
     2821 ccgtgttctg tatgctcttt cagtacaata agtgcgcgcc ccaattcaaa catgccttcc
     2881 atcgtctgcc gtaccgccaa gcggccgcgt tcgatccaag tggcttcgtt gtaaacctcg
     2941 ccgttacccc attgctccat caccatcacg ctgtgcgcga cttggtagtt ttgcactgaa
     3001 accgcatcca tcacttcaac ttcattgctc atttttcact ctccaaattt ccttacgtca
     3061 ggaaatttca aaattcgtta atctgcattc acccgcttgc cgatttcggc aatcttgctt
     3121 tgcagccgtt catgctgccg cctgaaccgc tcggcgattt gcagggtttt gataccgtag
     3181 gcgtagttgc cgttttcaag cttgatgacc aatcccgatg caaccaaatc atcaatatcc
     3241 ctgctgactt gtgatggcgt cagccccagt ccgaccgata aatccttatt gctcagaccg
     3301 ataatcggat gttcgtcaag cgcgataaag accctcaata gccgttgtac ccttttactt
     3361 tctgccatcc gcatcctcct tatttcagtc ccagcttctt ggcaatttcg taccccttgc
     3421 cgtaattgcc tttgcgctgt ccgccgatca ctagatacac atcgcgcggc ttaaagccgt
     3481 tctctcttgc ccactgcgcc agcgtctggc cgttttgggc aaaattttct ttcaattcgt
     3541 ctgccgtaag catttctttg gcctgtacgc gggaaaattt cagtatccgt ccgtacggtt
     3601 ggttaataca ctcatgatta atcatcgctt ttctccttca tccccagcaa aaccgccgct
     3661 tcgtggcttt tgccaaaatt gcctttcagc ttgccgcgca agaggtgctc caccgtggtg
     3721 cgctctagat tgaaatattt cgcccaatgc gccttgcaca ccccgttgcg cttaaaccac
     3781 ccggccgcgc tctcgcgcgt ttgcggatag ggaataggct tgaaattcaa aggtttttcc
     3841 atattattta tgcctttcgt gtgatataat gtgtttgttt ttaggtctct ttgcacgata
     3901 aacatccggt ctgttgtgca gcagcagggt cttggcttgt tttaaagtaa acaagctttt
     3961 tctcgtagct gggtgtatgt gatccatcat gagctgtcgc gcatgagctt tcaaagtgtt
     4021 cattttttcg tcctttctcg tgatgattta gggtgtttgt gtttcgatgt ggaaattata
     4081 ggaagaattc ttcctatttt gcaaggaata tttatgaata actcttcctt ttttggcaac
     4141 cgattgaaag aagaaagaaa aaaattaaaa atgactcaag ctgaaatcgc tgaaaaatgt
     4201 ggggtttcag gaagaatgtg gggggattat gaacgtggca tcagccagcc aaaagcggaa
     4261 cttttcttcc aatttgaaaa ggtgggtata gacgttcaat acgtcatgca cggcagacgc
     4321 ggcgaaacag cggtcatgcc gtctgaaacc ctgaacgccg aagaacaaga actgcttgcc
     4381 ctgttccgcg aggcggcagc tgccgaccgt gaaatgattc tgatggttgc gcgcagggca
     4441 gagaaaaaag cccaaactgc gcttggtaaa gtgagtaatg gataaaatga atcaattcga
     4501 attgtgccaa aaagaacatg tcaatccatt tgccttgtca aagcaatatc tattggttgt
     4561 aacattcgtc aaatccagca gtaaaaattt tcaggcagca ttactttggg caagaagtgc
     4621 caaattattt gagaatcttg agattggaaa agaaaccatc tattgctgcg ctttcgataa
     4681 aacagcagaa caggctggga tggccggggt atttttgaat tatattgaaa attggaatgg
     4741 caaacagatt tacatcaatg gccgaatcca tagtggcagt atttatgatt tgttaggggt
     4801 tttagactgc tatcaaaaat cacagtcctg tcccaaccct aaaagccact gttgctttgt
     4861 ttcagacgac atttttctat ggcatggatc aagaccaacg tttgaaatca gtcttgatct
     4921 aactggaaag aaaaaagaaa catcctctgc aaagaaattt gtgatgcctt gtattaattt
     4981 ccgtcaccat aggattgaaa aagaaaccta cttaggaaat tggaatgaac aaattgccgc
     5041 attggcagta aaacaaaata tagattggtg tccaagtttt gatattgaga attttagaca
     5101 gtatgaataa ttactatcta tataggaatt gcagcagcga tgtgttatgg gtcaaacgta
     5161 tccaacgcca aatcgacggc agcctactct tgatttctga caattcaacc tatccaccca
     5221 tgcccttggc actggcggaa caccccgata ttcaaatcat cgggcaggta gtgcaggtat
     5281 caaaagactt gaactagaca caatcaaaaa gggaaataga atgaaaatac tcgctttatt
     5341 aattgccgct acctgtgctt tatctgcgtg tggcggccaa gctgaagaac aaccggcatc
     5401 tgcccaacct caagagcagg cacaatccga attaaaaacc atgccggtaa gctataccga
     5461 ctatcaatca gtagccaata aagggcttgc cgaccaaaaa actggtctga cccttccaga
     5521 acatgttgtt cctgccgaca atgcggaagg gaagaatctg ctgcatgact tttcagacgg
     5581 cctcacatta accgttgata ccgataaagc cgacaaaatt actgctgtcc gagtagtctg
     5641 gaatacagat gcaatgcctc aaaaagcgga aaaactgtcc aaagctgccg cagccttgat
     5701 tgcggcaacc gccccgggag accgcacact gctgcgtgat accggcgacc aaatcaaaat
     5761 ggcaattgac agccataatg cgcaaaaaga gccaacccga gaatgggcgc gtggtgggat
     5821 tgcttataaa gtcactgtta ctaatttgcc gagcgtggtt ttgacagcaa aagctgaata
     5881 acctgcatta cagccacaat taggagcaga cggagatgat gtttatagag cctgctgaga
     5941 ataaataact gataaaaaag ctgtctgaaa aggtttcgga tagttaggat agctatctat
     6001 ttttaatata ttagagagaa tatgaaaaat aaactgactt tacgttattt caaaataact
     6061 cgttgcggtt attggtctgg ttcttcaaaa gaacctaatc attatgagga cttattaaaa
     6121 actttaactc atttagagac atatgttaaa ggtaaaacta ttgaagagac tggaattagt
     6181 cgtgatgata aagagccaag cactttcttg ctcgatatct gcaaaaaaaa tggtctgtgg
     6241 ttgcttgctt tttggaatcc tgccgcagaa agctcttcag atgaaattgt ttctttaaac
     6301 atgaaaagta aactaggtca agttgattct agcccagtaa cagcaggaaa tgggaaaact
     6361 tttggcttcg ttagttattt tcttatttgg ccagaacata atttttactc aacgattctt
     6421 tctcctaatg caactttagc tggacgaaca gagtttgaag agtacatgct taatttcttg
     6481 tggattgatc ctaattttat agaaaagact acctacgaag aaacttcaac agacggaata
     6541 attcatacca tggagcgaac taattttaaa ttgcctctta aaattaatgg aactcctgtg
     6601 ccacgttttg cggggaaact agctaaatct ccttcagaaa aagagtatgt cattagtaat
     6661 tatgaccaag taacccaagt gcataatcaa tgcacggtaa aggttacctc tggacaaagt
     6721 tcaagtggaa ttttgaagaa aatgttacca agcttttttg gttcaacaga attgtttgct
     6781 ccattaaaaa ccattcgtgt aaatcattct gtttctgctg catttgaaaa caaatcaaat
     6841 cttaaggatt ggattgaaga ctgggaaaaa aattcttttg atttagataa agatgattgt
     6901 ggctttaagt taaaaaacga tgacaagatt tattggattc gaggaaatgt catcaaatgt
     6961 gaattagacg tcaaccttca taaggaaaat gggatttatc cagctgctaa aattctagat
     7021 ataatagacc aaaggaaaga ttacgtttta aacgaattca aattaagtga tgattcttag
     7081 aaatattttc ctaatatgct ctgcattcat gattggtgta gcctggtatt taggtgctaa
     7141 tatccgattt gaagagcaat gggtattgta tgaagccttg agaacaactg catcaattat
     7201 ttttgcagtt gttggggttt ggttggctat tgtctatccc gaacgattaa aaagcccttt
     7261 tagtcaaact ttctcctctc cggtatatcg gcgtggattc aaacagcttt tttcccctgt
     7321 tataagttcc atttttattt taagtattgt gttacttgtt ggtttgttgg ctcctttgct
     7381 caaacatatt gaggcaataa ttcaatatat cccactttgt cggaaaatct catttagcct
     7441 gttagcaagc ttgacgatgt ggcaaattta tacggtttta tctgtattag tgcctttgtt
     7501 aaatatttta agtaaaaatg aaaatgactg tcatgcatcc aacattatta acaagctaaa
     7561 gggtgaacag aattagctat gtgattttta gtgtaaactg cttttaactc gtattaaaag
     7621 gccactcttt taatacctcc taaaatccct gtacctatca atagatacag ggatttttta
     7681 tgtccaccaa attcaaccaa ttcatcaacc gcgttctctc ccacgagggc ggttacgtca
     7741 gccatcccaa agaccccggc ggcgaaacca attggggcat cactaagcgc accgcccagg
     7801 caaacggcta caacggctcc atgcgtgcca tgacgcgtga acaggctatc ggcatttacc
     7861 gaaaggcgtt ttgggagcgt taccgcgccg accaaatgcc ggaagcggtc gcgttccaat
     7921 tttttgatgc ctgcgtcaac cacggttacg gcaatgccgt ccgtatgctg caacgcgccg
     7981 caggcgtggc ggacgatgga atcatcggcg aaatcagcct caaagccatc aattccctcc
     8041 ccgaaaacga ccttttattg cggttcaacg ccgagcgtct ggtcttttat accaagctca
     8101 gtacgttcac ctctttcggc aagggctggg tacgccgtgt ggcgcaaaac ctgattcacg
     8161 cgtctgcaga taacactgat taaagggaga taaaccatgt caaaaaagtc actcatcgcc
     8221 ctaatgaccg cagccatgca gcccgatttc agccacagcg acctaggcat tcgctacgcc
     8281 atgccgactc agggatgttg gacgcaagcc caccgcaaga gcggggtagc cgccgcgaaa
     8341 cgcgcagcca aaaaaaagcg tcacaaataa ccgccttttt ccgatggctg ggcggcttgg
     8401 tctctaatcc ggccacagga aaaatcagcc ataccaaact atgggcaaac gtcgccgcag
     8461 ccgccatgac ttggaagttc gtgcaggcgg cggacgcgcc cgaatggctc tagtgggctt
     8521 atggcgcatt ggtcggcggg tatgcattaa tcaaacgcgg catcgcggcg attccgcagt
     8581 tggcagaaat caaaaaatcc gcaaatcagg aaggggggcg gcaatgattg aatttgtccg
     8641 agccaaaaaa cggctgcttt gggcatttgt gcttttgctt gtgtggacgt gcggttaccg
     8701 atacgccgcc gacaaggccg aagcgaaaca aaccgccctg attgccacct atcggcattc
     8761 ttctatggtt gcggcggaac aatacgcctt gcagcttaaa aaagcgcagg acgaaaggca
     8821 gcggtggtac gacttttccc aaaaacaagg aagaaagccc gtgaaaaaac agtatccgcc
     8881 gcaaacgaaa aaagccggct atctgaaaac caaggaagaa ctgcttgcgg aattggcttg
     8941 ccttaaagcg gaaatggctg ccctaaaaaa gctcgatgcc ttaatctatg ggaaagaagt
     9001 gcggcagaaa gaacgcaact cgtcgcaggg ttaaggcaat gccatccgtt gaactgctgt
     9061 tggtgattgt cggactgcca cgcggcacct tctattacca attggccgtc caatcggcag
     9121 aagacaaata tgccgatttg aaacggcata tccatgatat ttatcgacga cataagggaa
     9181 gatacggcta ccggaggatt gcggcagcca tccgtcacgc aggaacaccg gtcaatcaca
     9241 agaaagtcag ccgtctgatg gcgaagacgg ggctgaaggc agtgatacgg cggcgcaaat
     9301 accgctcgtt caaaggagaa gtcggcaaaa ttgcgccgaa tatcctgcga cgctgtttcc
     9361 atgcagaaaa gccgaatgag aaatgggtaa cggacgttac cgagttcaat gtaggcggag
     9421 aaaagatata cctttctccg attatggatt tgtttaacgg ggaaatcgtc agttaccgta
     9481 ttcaaacccg cccgactttc gatttggccg acgagatact gaaaggtgcg ccggagaaac
     9541 cgggatcgtc tgaaaagccg atactgcatt cggatcaagg ttggcaatat cagatgtttt
     9601 tttatagtgg attaaattta aatcaggaca aggcgatgaa gccgcagaca gtacaaatag
     9661 tacggcaagg cgaggcaacg ccgtactggt ttaaatttaa tccactatac aacaacgata
     9721 gaatcaagtt gaaattaaaa gggctgagcc ttgttcagta cagaattcag tccctgaaag
     9781 ccgcttgatt aaactgtccg actttttggg gtcagttcgg cttcggcatt tttttatccg
     9841 tttgggggta acttgtttgg aaagctgcaa gcttataaat aaaggattac atttaagttt
     9901 tgggtaacct ttttaaaaaa atgcgtgatg acttttgcat ttttaaggcg ttttttgggg
     9961 taattcgtga aaagttaccc caaaagttac cccataaatg gcgaaaactc aagcatacgc
    10021 cagcatattc aaggcaacaa aaaagccttg aaactgttga agttcaaggc ttttttgtgt
    10081 tgcaggatgc tgctgaaaat agggtatggt ggaggcgggg ggaatcgaac ccccgtccga
    10141 aagtcctcta caaagcgttc tacatactta gttgtgtcta tttgaaaatc ttatttccat
    10201 catgccgacc aacaggcctt ttggaaacca gttaccttaa atctcatttc ctgccaagta
    10261 acccggtagg aaaccagtca atgtaagatg acgttgcggt ggctttcgcc acacagccca
    10321 ttgaccgact gctgcaacgg ctagccttaa gcggctaaag cgtaagtttc gtcgtttgcg
    10381 actatttgaa ttcagtgttt tacgggaatc tgagaccccg gtatgcccgc atctgcttcg
    10441 caacccccgt cgaaaccaag gtcgccccca gaaatggttt gcaaattata cggatattgt
    10501 gcggtgctgc caagtctgtc ggagaaattt gtcagtcttg ctgccttaat ttgcgtttga
    10561 gcaggatgcg gacgcagccg tcgttgcctt cccggggttc ggcgtaggcg agtacgtcgg
    10621 ggtgttgcat cagccagttt cgggtcatat ttttcagaac gggtttgtag cctttggaac
    10681 ctaatccgct gccgtggatg atttcgccgc atacgccgcg tttttgggtg aatgcgatga
    10741 attcgttgag gactttttgg gcttcttcct gtgtgtagcc gtgcaggtcg acatcggtaa
    10801 cgacgggata gtatccgttt ttcaggcgtt ggatgtcgtt ttttccctgt ccgtttttgc
    10861 tgaagctggc gggcgggtcg ttgtatgtgc tgccgatgta gaagtagttt tcttcgtcgg
    10921 cgcggttgtc tttgggacgg actttgatgg ggggtttgtc gggcggcgca taatattgct
    10981 gccggttttt taatggggag acttgtccga ctgcttgtga aaaatcgaaa tcctgttctt
    11041 gtttttgctt gtttttttcg gctgcctgtt tttctgccgc ttctttttgg gcttgtttgc
    11101 ccagttgttt gaggatgttt tggaagtcgg tattcatatt ttttcctgtt atttgtccga
    11161 tggctgtttc gggcggggtt ttaatttgcc ggaatgtttg ccaatcgggg gaggatgatt
    11221 ttgttgcctg cgtatgtttt ttgaaagtgt gattgtatat caaaaagaaa tgcggcaacc
    11281 gtcggcagtg ttgattgccg gaaatgcgga ccggtcgaac cgatatgccc gaacgcctga
    11341 taaagtttta aaaacctgcc ttgcgaagca ggctgacgtg ttttgccaat cttgaattgc
    11401 cggaaacgcg aaacacggaa atctga
//
LOCUS       DAXHPG010000052        11352 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXHPG010000052 DAXHPG010000000
VERSION     DAXHPG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.052"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            204..1637
                     /gene="lpdA"
                     /locus_tag="ACFM6F_001038"
     CDS             204..1637
                     /gene="lpdA"
                     /locus_tag="ACFM6F_001038"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6164470.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDIKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLNIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            1762..2049
                     /locus_tag="ACFM6F_001039"
     CDS             1762..2049
                     /locus_tag="ACFM6F_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164471.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            2103..3314
                     /gene="sucC"
                     /locus_tag="ACFM6F_001040"
     CDS             2103..3314
                     /gene="sucC"
                     /locus_tag="ACFM6F_001040"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC6164472.1"
                     /translation="MEANSGHSTNLKEKSMNLHEYQAKELLASYGLPVQGGILAHNGE
                     EAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTD
                     ANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKI
                     FKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLA
                     VRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIG
                     CMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINI
                     FGGIVRCDMIAEAIVAAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLND
                     AAEKIVAAVNA"
     gene            3325..4215
                     /gene="sucD"
                     /locus_tag="ACFM6F_001041"
     CDS             3325..4215
                     /gene="sucD"
                     /locus_tag="ACFM6F_001041"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC6164473.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVSKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKTKGLA"
     gene            4244..4384
                     /locus_tag="ACFM6F_001042"
     CDS             4244..4384
                     /locus_tag="ACFM6F_001042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164474.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFLPFEAGRIFIGQTGA
                     NP"
     gene            complement(4450..4779)
                     /locus_tag="ACFM6F_001043"
     CDS             complement(4450..4779)
                     /locus_tag="ACFM6F_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6164475.1"
                     /translation="MDVQKQYSFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPYTKNLSAGKQRQHERGIWQRRFYGHTVRDETDLQRCADYIHSNPIKQKMSRA
                     KTESCKK"
     gene            5028..5327
                     /locus_tag="ACFM6F_001044"
     CDS             5028..5327
                     /locus_tag="ACFM6F_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164476.1"
                     /translation="MKRQTVNQATACVRTAHTLHVDFEVPCNSQVGCVAQPRTRCWVC
                     RLRLAAAETPEEKAKDAAEEAKEEAKDAAEEAKEEAKDAAEDAKEAAEDAKEEAK"
     gene            complement(5554..6495)
                     /locus_tag="ACFM6F_001045"
     CDS             complement(5554..6495)
                     /locus_tag="ACFM6F_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HFC6164477.1"
                     /translation="MEYNAIMKTIAPSHIKPLMPELNQSQRNRIIFIDFYLCFLGKIN
                     RKDLMQAFQISPAVATRDFALYCSSFSKNLRFDTKSKFYLSTEDFEPQFPHSFENAIK
                     ILNGSLIFDIDSQHQPLLPFDLPPVLINPDVLIFSKISKALSLKKPLKISYHSISSGL
                     SEKVIVPLALVNDGFRWHLRAFDRTKNRFADFVLTRIIQANILLAENIEENETINNDI
                     DWNRIVKIELVPHPNHDEAKKVIEMDFGMTNGKLELALRASNIGYVLQQWRVDCSSDH
                     HIDDPAFRLWLLDNLVLYGINNAEHLAPAFDKKRSKP"
     gene            6633..6902
                     /locus_tag="ACFM6F_001046"
     CDS             6633..6902
                     /locus_tag="ACFM6F_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164478.1"
                     /translation="MKIKFAHLRHPAVGGGYINFAVFDAKSTNGNHSVVLKELTTEAR
                     RNGLKIDQSALIFEQHGRTQFYGDKNLVDFLSANWYPQWTHTLTI"
     gene            6918..7103
                     /locus_tag="ACFM6F_001047"
     CDS             6918..7103
                     /locus_tag="ACFM6F_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164479.1"
                     /translation="MTNKIKPGQNTGNNGGIYQEIGSRGGRKDNYATIKDNQKAPPTQ
                     NPGSSWIQIKRTPDSSR"
     gene            7118..7288
                     /locus_tag="ACFM6F_001048"
     CDS             7118..7288
                     /locus_tag="ACFM6F_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164480.1"
                     /translation="MFLGIFIALSGILTLLEMFNVISSDMKWGMPLMFTCLGFSIIFD
                     SFKAKQNRKQKE"
     gene            complement(7320..10166)
                     /gene="uvrA"
                     /locus_tag="ACFM6F_001049"
     CDS             complement(7320..10166)
                     /gene="uvrA"
                     /locus_tag="ACFM6F_001049"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC6164481.1"
                     /translation="MCNHHPRHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVITGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAIEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIDRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIASGSPEEVAKVKGSYTGKYLKNIL"
     gene            10321..11118
                     /locus_tag="ACFM6F_001050"
     CDS             10321..11118
                     /locus_tag="ACFM6F_001050"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC6164482.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTEVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLAAP
                     QIESEPESEPALQTAPVETAATPSAEQRQIEAAAAKIQAAVQDVLKD"
BASE COUNT         2938 a         2578 c         2982 g         2854 t
ORIGIN      
        1 gatgtagcgt aatgtagtat cgtgctacaa taggctcagc gaacgattga ggccgtctga
       61 aacatttgat tcgaatgaat cggcagatat agactttcag acggcctttt cttaaaaccg
      121 tcaaaatgca gtcattcaaa ataaaaaaga aacaaaaagt atcgttttta ttttgagata
      181 ctgcaaaagc aaaggatgac acgatgtctc aatatgatgt agtagtgatt ggtgcaggcc
      241 cgggcggata cgttgccgcc atccgtgccg cgcaactggg tttcaaaacc gcttgcgttg
      301 acgcaggggt taacaaagca ggcaatgccc ctgcattggg cggtacttgc ttgaacgtag
      361 gctgtattcc ttccaaagcc ctgttgcaat ccagcgaaca tttccacgcc gcgcaacacg
      421 agtttgccga acacggtatc actgtcggcg acataaaatt cgacgcggcc aaaatgattg
      481 agcgcaaaga tgccatcgtg accaagctga ccggcggcgt gaaattcctg ttccaaaaaa
      541 acaaagtaac cagcctgttc ggtactgctt cttttgccgg taaaaatggc gatgcttacc
      601 aaatcgaagt cgataacaaa ggcgagaaaa ccgttatcga agccaaacac gtcatcgtag
      661 caaccggttc cgtaccgcgt ccgctgccac aagtcgctat cgacaacgtg aacgtattgg
      721 acaacgaagg cgcattgaac ctgaccgaag tgcctgccaa actgggcgtc atcggttccg
      781 gcgtgattgg tttggaaatg ggttccgtat ggaaccgcgt gggtgcagaa gttaccattc
      841 ttgaagctgc gccgaccttc ctggctgccg ccgaccaaca aatcgccaaa gaagccttca
      901 aatacttcac taaagagcag ggcttgaaca tcgaactggg cgtgaaaatt ggcgacatca
      961 aatctgaagg caaaggcgtt tccgttgctt acgaaactgc tgccggcgaa gctaaaaccg
     1021 aagtatttga caaactgatc gttgccattg gccgtattcc aaacaccaaa ggtctgaacg
     1081 ccgaagccgt aggcttggaa aaagacgagc gcggtttcat caaagtagat ggcgaatgcc
     1141 gtaccaacct gcctaacgta tgggcaatcg gcgacgtggt tcgcggcccg atgttggcac
     1201 acaaagccag cgatgaaggc gttgccgttg ccgagcgcat cgccggtcaa aaaccgcata
     1261 tcgacttcaa caacgtaccg ttcgtgattt acaccgatcc tgaaatcgct tgggtaggta
     1321 aaaccgaaga gcagctcaaa gccgaaggcg tggagtacaa aaaaggtact tcaggtttcg
     1381 gtgcaaacgg tcgcgcattg gcaatgggca aagccaaagg tacggttaaa gtattggcag
     1441 atgccaaaac cgaccgcatc ttgggcgtac acatgattgg tccggttgtc agcgaattgg
     1501 ttaccgaagg cgtgactgcg ctcgaattct tcgccagcag cgaagacatc gcccgcatta
     1561 tccatgccca cccgaccttg tccgaagtgg ttcacgaagc tgcattggcg gccgacaaac
     1621 gcgctttgca cggttgatag atattaaggc cgtctgaaac ttttcagacg gccttaaggc
     1681 tttcgacaaa ttgaatgttc cgagagcttc gttttctgat ttataattcc gtcagacaaa
     1741 caaacagcat ttacattcgt tatgaacaaa gaaatagtcg gtattttctt tataccgatg
     1801 ggcatcatca gcatgtgtat ggccgcattg tggcagatgt atgtgatgat gaccgaaact
     1861 tatacgctca accgtttcaa agataaagaa ttggtttggc gcgtggcatt gttgtttatc
     1921 agtttcagcc ttgccgttta tctgctctgt ccgaattcgc gtaaaaaagg catcgtcttt
     1981 tttattctcg ggggaggcgg tgcagccatg tatctgctgg cgcggatgtg gttgcctttc
     2041 agcaagtgaa acgacgattt tccgaccgcc gaaaggtagt ctgaaacgca cgggcttgcc
     2101 atttggaggc aaactcgggg cattccacta atctaaagga gaaatccatg aatttacacg
     2161 agtatcaggc taaagaactg ctggctagct acggtttgcc cgtacaaggc ggtattttgg
     2221 cacacaacgg cgaagaagcc gctgcagctt acgacaaatt gggcggcaaa ttcgctgttg
     2281 tcaaagcaca agtacatgcc ggcggccgcg gtaaagcggg cggcgtaaaa gtcgttaaaa
     2341 gccgcgaaga agctaaagaa gtggctgaaa gcctgattgg caccaacttg gtaacttacc
     2401 aaaccgatgc caacggccaa cctgtcaaca gcgttttggt ttgtgaagat atgtatccgg
     2461 ttcaaaccga gctgtacttg ggcgcagtgg ttgaccgttc tacccgccgc gttacattca
     2521 tggcttctac cgaaggtggc gtagaaatcg aaaaagttgc tgccgaaact ccagaaaaaa
     2581 tcttcaaagt aaccgttgat ccgctggtcg gcctgcaacc ttgccaagct cgcgaagttg
     2641 ccttccaact gggcttgaaa gacaaacaaa tcaacgagtt cgtcaaactg atgaccggcg
     2701 cgtacaaagc gtttgtcgaa aacgacttcg ccctgtttga agtcaacccg ctggcagttc
     2761 gcgaaaacgg cgcgctcgcc tgcgtggacg gcaaaatcgg catcgacagc aacgcgctct
     2821 accgcctgcc aaaaatcgct gaattgcgcg acaaatctca agaaaacgaa cgtgaactga
     2881 aagcttctga attcgacctg aactatgttg ccctggaagg caacatcggc tgtatggtta
     2941 acggtgccgg tttggcgatg gccaccatgg acatcatcaa actcaaaggc ggtcaacctg
     3001 ccaacttctt ggacgttggc ggcggcgcaa ccaaagaccg cgtggttgaa gcgttcaaac
     3061 tgattttgga agacaaatcc gttaaaggcg tattgatcaa catcttcggc ggtatcgtac
     3121 gttgcgacat gattgcggaa gccatcgtgg cagccgttaa agaaatcaac gtcgacgttc
     3181 ctgtcgttgt tcgtttggaa ggcaacaacg ccgaactcgg cgcgaaaatc ctgaacgaat
     3241 caggtctgaa actgacttct gcagacggcc tgaatgacgc agccgaaaaa attgttgcag
     3301 ccgtaaacgc ctaaggagaa aagaatgagc gtattgatta ataaagacac caaagtattg
     3361 gttcaaggtt tcaccggtaa aaacggtact ttccactccg aacaagctct ggcttacggc
     3421 actaaagttg tcggcggcgt taccccgggc aaaggcggtc aaacccacct gaacctgcct
     3481 gtattcaaca ccatgaaaga agccgttaaa gaaaccggcg cggatgcatc tgtaatttac
     3541 gttcccgctc cgtttgtgtt ggattctatc gttgaagccg ttgattccgg cgtaggcttg
     3601 gtggttgtga ttaccgaagg cgtgccgact ctggacatgc tcaaagccaa acgttacttg
     3661 gaaaccaacg gcaacggtac ccgcttggtc ggccctaact gcccgggcgt gattacccca
     3721 ggcgagtgca aaatcggtat tatgccgggc cacatccaca ctccgggccg catcggtatt
     3781 atttcccgtt ccggtacatt gacttacgaa gccgtggcac aaaccaccaa actgggcttg
     3841 ggtcaatcaa cctgtatcgg tatcggcggc gacccgattc ccggtatgaa ccaaatcgac
     3901 gcactgaaac ttttccaaga agacccagat accgacgcca tcatcatgat cggtgaaatc
     3961 ggcggtactg cggaagaaga agcagccgaa tacatccaat ccaacgtaag caaacctgtt
     4021 gtcggctaca tcgccggtgt taccgctcct aaaggcaaac gcatgggcca cgccggtgcg
     4081 attatttccg gcggcaaagg tactgccgag gaaaaattcg ccgcgtttga aaaagccggt
     4141 atcgcttaca cccgcagccc tgccgagttg ggcactacca tgctggaagt gttgaaaaca
     4201 aaaggtttgg cataatcagg tttgacaact gattgaacat caaatgccgt ctgaaaccgg
     4261 aaatcgggtt tcagacggca ttttgtttgt catttcaaaa agaggcagcc tcaacatacc
     4321 cacattattt ttgccctttg aggcaggcag aatatttatc ggtcaaacag gagcaaaccc
     4381 gtaagtcagg ttgcacacgc cttgtttgaa gttggggaat gataactatc gcatttgaaa
     4441 agactgatat tattttttgc aggattctgt tttagcacgc gacatttttt gtttgatagg
     4501 attggaatgg atgtagtccg cgcaacgctg caaatccgtt tcgtcgcgca cggtgtgtcc
     4561 gtaaaaacgc cgttgccaaa taccgcgttc gtgctgccgc tgtttgccgg cggataagtt
     4621 tttagtataa ggggaatatg cggagaattt ggttttaatc agccgccgga gtagggaata
     4681 atccgcatcg tcgggcggca gcgtccaaat ggcgtgaata tggttcggca gcacgcatac
     4741 ggcgacggtt tcaaaggaat attgtttttg cacatccata taagccgccc gcgaagtcga
     4801 tatgttcgac aagcaggcgc gatttcgggt cggcgagttt gacggtgaaa aagaatgtgc
     4861 cgccggcaat gaagtttctg cgataacgcg ccataggatt tttgtttttg gtttgagcgt
     4921 tggagaagga atggcttgtt gtttaaggtt tttaacttcg ttgaagctag cgctttcaga
     4981 cgacattgga ggccgtctga aagcgctagc ttcaacgaag ttaaaagttg aaacggcaaa
     5041 ccgtcaatca agcaaccgcg tgcgtgcgta ccgcacacac cctacacgtt gattttgaag
     5101 ttccgtgtaa ttcacaggta gggtgtgtgg cgcagccacg cacgcggtgt tgggtatgca
     5161 ggctacggct tgctgctgca gaaactcctg aagaaaaagc caaagacgcc gctgaagagg
     5221 ctaaagaaga agcaaaagac gccgctgaag aggctaaaga agaagcaaaa gacgcagcgg
     5281 aagatgccaa agaagctgct gaagacgcta aagaagaagc taaataattc ggatagcttt
     5341 acagaaaaac ggattccaat gattgggaat ccgttttttg ttttgtgagt tagggtagtt
     5401 gttttagata aagctttttc agttttcaga cggccatttt tcatgccgtc tgaaagtgtt
     5461 agttttaact aagttcaatt tacaacagaa agccatcaat caaacaaccg cgtgtgtggc
     5521 gcagccacgc acgcggtcgg ttggatatgt aggctacggc ttgctacgtt ttttatcaaa
     5581 ggcaggcgct aagtgttcag cattgttgat gccgtataaa actaaattat ctaacagcca
     5641 taatctaaaa gcaggatcat cgatatgatg atctgacgaa caatcgactc gccattgctg
     5701 taaaacatat ccgatatttg aagctctaag cgccaattct aattttccat ttgtcattcc
     5761 aaagtccatt tcaatcactt ttttagcttc atcatgatta ggatggggaa ccaactcgat
     5821 tttaacaatt ctattccaat caatatcatt gttaatcgtt tcattttctt caatattttc
     5881 agctaataaa atatttgctt gaattattcg tgttaggaca aaatctgcaa atcggttttt
     5941 tgttctatcg aatgcccgta aatgccatcg aaatccgtca ttaactagag caagaggcac
     6001 aataactttc tcagataatc cagatgaaat tgaatgatat gagattttta gaggtttttt
     6061 taaggataat gctttcgata ttttagaaaa gattaagaca tcaggattta ttaaaacagg
     6121 aggtaaatca aacggaagaa gaggttgatg ttgggaatca atatcaaata ttaaactgcc
     6181 attaagaatt ttaattgcat tttcaaatga atgggggaat tgaggttcaa agtcttcagt
     6241 agagaggtaa aatttacttt tggtatcaaa acggagattt ttcgaaaaac tagagcaata
     6301 tagcgcaaaa tccctagtag caacagctgg ggaaatttga aatgcctgca tcaaatcttt
     6361 tctatttatc tttcctagaa agcatagata gaaatcaata aatataatcc tattgcgttg
     6421 agattggttc aactctggca taaggggctt tatatgggaa ggagcgattg ttttcataat
     6481 tgcattatat tccatcaatc tttttcatgg caatatgaat tcaaaaaaat tgcttgacgt
     6541 aatcaaaaag agtgcatata atgcaaaaag ctacttcata gtagctctct gacttaaaat
     6601 ttctgttatg taatttttta taggatttat ttatgaaaat aaaatttgct cacttaagac
     6661 atccagccgt aggtggagga tatattaact ttgctgtttt tgatgctaaa tcaactaatg
     6721 gtaaccatag tgtcgtactg aaggaattga ccactgaagc tcgtagaaat ggattaaaaa
     6781 ttgaccaatc tgcgttaata tttgaacaac atgggcgtac acaattctat ggagacaaaa
     6841 atctagtaga ttttttatct gcaaattggt atccgcaatg gactcatact ttaactattt
     6901 aaattatgga gatgataatg acgaacaaaa tcaaacctgg tcaaaataca ggaaataacg
     6961 gtggaattta tcaagaaatt ggttcacgag gtggtcgaaa agataactac gcaaccatta
     7021 aagataatca aaaagcacca cccactcaaa atccaggaag ctcatggatt caaattaaaa
     7081 gaactccaga tagctccaga taataaggat agttctaatg tttttaggta tttttatagc
     7141 tttatcaggc attcttacat tattagaaat gtttaatgtt atttcttcgg atatgaaatg
     7201 ggggatgcct ttaatgttca cctgtttggg atttagcatt atttttgata gcttcaaagc
     7261 caaacaaaat cgaaaacaga aggaataaaa aaacgggatg gtttaaacca tcccgttttt
     7321 taaagtatat tcttcaaata cttcccagta taactcccct taaccttcgc cacttcctcc
     7381 gggctacctg aagcaataat cctccctccg ccgtcgccgc cttctggccc taagtccaca
     7441 atccaatcgg cggttttaat cacatccaga ttatgctcga taatcactat cgagttgcct
     7501 ttgcctttca gacggtctat gacttccagc agcagggcga tgtcggcgaa gtgtaggccg
     7561 gtggtgggtt cgtcgaggat gtagagcgtt ctgccggtgt cgcgtttgga gagttccaag
     7621 gcgagtttga cgcgctgggc ttcgccgccg gagagggtgg tggcggattg gccgagacgg
     7681 atgtagccta agcctacgtc catcaaggtt tgcagtttgc gcgatacggt ggggacggcg
     7741 tcgaaaaatt cacgggcttc ttcgacggtc atgtcgagga cttggctgat gtttttgcct
     7801 ttgtattgga tttcgagcgt ttcgcggttg tagcgtttgc cgtggcagac ttcgcagggg
     7861 acgtacacgt cgggcaggaa gtgcatttcg actttgatga cgccgtcgcc ttggcaggct
     7921 tcgcagcggc cgcctttgac gttgaaggag aatctgccga cgttgtagcc gcgttcgcgc
     7981 gagaggggta caccggcgaa gagttcgcgg atgggggtga acaggccggt gtaggtggcg
     8041 gggttggagc gcggggtgcg gccgatgggg gattggtcga cgttgatgac tttgtcgagg
     8101 tgttcgaggc cgcggatgtc gtcgtatggg gcgggttctt cttgggcgcg gttgagttcg
     8161 cgggcggtga ttttggcgag ggtgtcgtta atcagggtgg atttgccgct gccggatacg
     8221 ccggtgatgc aggtaatcaa accgagcggc agctcaaggg taacgttttt gaggttgttg
     8281 ccgcgcgcgc ctttgaggac gagcattcgg tcgggattga ctggcgtgcg ttcagacggc
     8341 acggcaatgg attttttgcc gctgaggtat tgtccggtaa cggagttttc gcatttggcg
     8401 acgttttcgg gcgtgtcggc aatcaatacg ttgccgccgt gttcgcctgc gcccgggccc
     8461 atatcgacga cgaaatcggc ttcgcggatg gcgtcttcgt cgtgttcgac cacgatgacg
     8521 ctgttgccca aatcgcgcag gcgtttgagg gtggcaagca ggcggtcgtt gtcgcgctgg
     8581 tgcaggccga tggagggttc gtccaaaacg tacatcacgc cggtcaggcc gctgccgatt
     8641 tggctggcga ggcggatgcg ctgggcttcg ccgccggaaa gggtttcggc ggaacgggag
     8701 agattcaggt aatccagccc gacattaatc aaaaagccga gacgttcggt gatttctttg
     8761 aggatttttt cggcgatttg ttttttgttg ccgtccaaat ccagcgtttc aaagaatcgg
     8821 tgggttttgg tcagcggcca ggcggaaact tcgtgcaacg gctcgccgcc gacgtaaacg
     8881 tagcgggctt ctttgcgcaa acgtgcgccg ccgcagctcg ggcaagcgcg gtggttttgg
     8941 tattcgcgca gtttttcgcg cacggtttcg ctgtcggttt cgcggtagcg gcgttcgaga
     9001 ttggggatga tgccttcgaa ggcgtggctg cggttgaagg tggtgccgcg ttcggacagg
     9061 taagtgaaat caatgacttc tttgcccgag ccgtgcaaca cgactttttt gatttttgca
     9121 ggtagggttt cccaagcagc ctgcacgtcg aaaccgtaat gccgcgccag cgactgaatc
     9181 atttggaaat agaactggtt gcgcttgtcc cagccgtcaa tcgcgcccga agccagcgac
     9241 aattcgggat gcgcgaccac tttttcggag tcgaagaaat tggtgttacc caagccgtcg
     9301 caagtcgggc aggagcccat cgggttgttg aacgaaaaga ggcgcggctc caattcgggc
     9361 aggctgtacg aacatacggg gcaggcgaaa cgcgcggaaa accaatgctc ttcgccgctg
     9421 tccatctcta tcgccagtgc gcgttcgttg ccgtggcgca gagcggtttc aaaactttct
     9481 gccagccgtt gcttgatgtc agcttttact ttcacgcggt cgatgacgac atcgatattg
     9541 tgcttgatgt ttttttccag cttcggcact tcgtcaagct gatagacctc gccgtccact
     9601 cgcacccgtg caaagccctg cgcctgcaag tcggcaaaga aatcgacaaa ctcaccttta
     9661 cgctcgcgta ccgccggcgc cagaatcatc acgcgcgtgt cttccggcag cttcaacacg
     9721 gcatcaacca tctgcgacac ggtctggctg gatagcggca gattgtgttc ggggcaatac
     9781 ggcgtgccga cgcgggcgta caaaaggcgc aggtaatcgt ggatttcagt taccgtgccg
     9841 acggtggaac gcggattgtg gctggtggat ttttgctcga tggaaatcgc gggcgagagg
     9901 ccttcgatca aatcgacatc gggtttgtcc atcatctgta aaaattgacg ggcataggcg
     9961 gaaaggctct cgacataacg ccgctgccct tcggcataca gcgtgtcaaa tgccagcgat
    10021 gatttgccgc tgcctgacaa tcctgttatg accacgagct tgtggcgcgg aatgtccaaa
    10081 tcgatgtttt tcaaattatg cgtgcgtgcg ccgcgtatgc ggatggtgtc gttgtcgtgc
    10141 gaatgtcggg gatgatggtt gcacataatg gatgccgcct gaaaaataaa ggaaaaccga
    10201 tattgtagca ctttctcgga tgctgtctga agccgcgttc agacggcatt tgtcggcgga
    10261 atgcggcgga ttccgttata atggcggcaa ttttaacccg cttgaataga aggatgacaa
    10321 atgaaccgtc tttaccccca cccgattatc gcccgtgagg gctggccgat tatcggcggc
    10381 ggtttggctt tgagcctgct ggtgtcgatg tgctgcggct ggtggtcttt gccgttttgg
    10441 gtgtttaccg tatttgcatt gcagtttttc cgcgaccctg cgcgtgagat tccgcaaaat
    10501 cctgaagcgg tgttgagccc tgttgacggc cgcattgtgg tggtcgagcg cgcacgcgat
    10561 ccgtatcgtg atgtcgatgc tttgaaaatc agtattttta tgaacgtgtt caacgtgcat
    10621 tcgcaaaaat cgcctgccga ttgtacggta acggaagtgg tctataacaa aggcaaattc
    10681 gtaaatgcgg atttggacaa agccagcacg gaaaacgaac gcaacgcggt tttggcgacg
    10741 acggcttctg gtcgtgaaat tacttttgtt caagtggccg gtttggtggc gcgccgtatt
    10801 ttgtgctaca cccaagcagg tgcgaaactg tcccgcggcg aacgctatgg ctttattcgc
    10861 ttcggttcgc gcgtggatat gtatctgcct gtcgatgcgc aggcgcaagt ggcgattggc
    10921 gataaagtaa ccggcgtcag cactgtattg gcgcgtttgc cgctggctgc gccgcaaatc
    10981 gaatccgagc ctgaatctga gcctgcttta caaactgctc cggttgaaac agcggcaacc
    11041 ccatctgccg aacaacggca aatcgaggca gcggcggcta agattcaggc ggctgtgcaa
    11101 gatgtgttga aagattaatt ttgcgaactg aaatagaaaa tatcagtacc atcattcaca
    11161 cgaatgagga agtttggttt tttgaatttt tgctaatgtt cacaccgtta tgttcacgaa
    11221 agtggaaatc tgggaactta acgttacggt gatttatcag aaataacaga aaccgaacga
    11281 attgtattcc cgcctgcgcg ggaatgacga cttattagtt acctaaaact taaaaaacag
    11341 aaacctttcc gc
//
LOCUS       DAXHPG010000053        11171 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXHPG010000053 DAXHPG010000000
VERSION     DAXHPG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.053"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            142..1539
                     /gene="aspA"
                     /locus_tag="ACFM6F_001051"
                     /pseudo
     CDS             142..1539
                     /gene="aspA"
                     /locus_tag="ACFM6F_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
     gene            complement(1749..2624)
                     /locus_tag="ACFM6F_001052"
     CDS             complement(1749..2624)
                     /locus_tag="ACFM6F_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC6164483.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQIAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRAESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2626..3348)
                     /locus_tag="ACFM6F_001053"
     CDS             complement(2626..3348)
                     /locus_tag="ACFM6F_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC6164484.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQAHREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3371..3850)
                     /gene="ybaK"
                     /locus_tag="ACFM6F_001054"
     CDS             complement(3371..3850)
                     /gene="ybaK"
                     /locus_tag="ACFM6F_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC6164485.1"
                     /translation="MSKHTYPITPAVRVLRENGIKFEPFIYPYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKRIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3925..4287
                     /locus_tag="ACFM6F_001055"
     CDS             3925..4287
                     /locus_tag="ACFM6F_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6164486.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKKDAATA"
     gene            4322..5185
                     /locus_tag="ACFM6F_001056"
     CDS             4322..5185
                     /locus_tag="ACFM6F_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC6164487.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPNLAFAAQQDSDGICSIALPHGGILRIAPKNTLPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALAVIKFSAAQKELAAPNGGIFKA
                     VHLFFKTENAE"
     gene            5279..6238
                     /gene="ttcA"
                     /locus_tag="ACFM6F_001057"
     CDS             5279..6238
                     /gene="ttcA"
                     /locus_tag="ACFM6F_001057"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC6164488.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(6332..9271)
                     /locus_tag="ACFM6F_001058"
     CDS             complement(6332..9271)
                     /locus_tag="ACFM6F_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFC6164489.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRAADENPELLFAA
                     KQYADNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTRQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFAKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSGGKEAKFELNEKDKKQTFKLAKGEDLAKIHPAVSDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIHAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLGLLNKLFSDGIIDDNENFTE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMTYRELLEKLAQTAKIRMQTLHQAFYRVRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYQLVGSKIHPTKFTDKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTQSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9284..>11171)
                     /locus_tag="ACFM6F_001059"
     CDS             complement(9284..>11171)
                     /locus_tag="ACFM6F_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6164490.1"
                     /translation="PDLIDTAGVNTEAIYTSDGITANSTQLEQLKKLFPACFDADGNF
                     LIDRLQAEIAPQTDIGREFYEMNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQ
                     NLLIRGDNLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELACLANI
                     DEDEAARILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLL
                     CDEVFGEGNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKK
                     IVINDELYWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSY
                     GKTIIGKLRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPL
                     DLLKRLVLSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTA
                     EKSEARKAGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDF
                     SPEQPDLPLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGAYTAYLCEKRLYLMN
                     AGFTSADLLAFIRKLDDDADFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNV
                     IIRV"
BASE COUNT         2697 a         2976 c         2624 g         2874 t
ORIGIN      
        1 cccgaaaaca acgtttcccg ttttcagacg gcatacgata tggacaattt catccggccg
       61 gtatagtgtt cgtcctgcgg cggcatcttt ccgatggcgc attttttcaa tattaattat
      121 ccgacttaca aaggtaaaaa catgactgtc cgtatcgaac acgatttatt gggcgaccgc
      181 gagattcccg ccgaagtgta ttggggcatc cacaccctgc gcgccattga aaactttaaa
      241 atctccacac aaaaaatttc tgacgtgccg cagtttgtcc gcagtatggt gatggtgaaa
      301 aaagcaaccg cgcaggcaaa cggcgaattg ggtgcagtaa aacccgaaat cgccgccgcc
      361 attgaaaagg cttgcgacga agttctgctg aacaaccgct gcctcgacca attcccctcc
      421 gacgtgtatc agggcggtgc gggtacttcg gtcaatatga acaccaacga agtcatctcc
      481 aacctcgcat tggaagcctt gggatacgaa aaaggccgct acgacatcgt caatccgatg
      541 gatcacgtca acgccagcca atccaccaac gatgcctacc ctaccggctt ccgccttgcc
      601 gtgtattaca gtatcggcga attgctcgac aaactggtcg ttctgaaaaa cgcctttgcc
      661 gccaaagccg aagaatttaa agacgttttg aaaatgggcc gcacccagct tcaagatgcc
      721 gtgccaatga ctacaggaca agaattccaa tctttccaag tgttgttgga agaagaaatc
      781 ctcaacctcg accgcacgcg ccaactgctt ttataagtca atttgggcgc aacggcaatc
      841 ggcacgggcg tgaacacgcc caaaggctac gccgaactgg tggtcaaaaa actctccgaa
      901 gtcagcggct tgccttgcaa actgaccgaa aacctgattg aggcgacctc cgactgcggc
      961 gcatatgtga tggtacacgg cgcattgaag cgcacggcgg tcaaactctc caaaatctgc
     1021 aacgacttgc gcctgctctc ttccggcccg cgtgccggct tgaaagaaat caacctgccc
     1081 gaattacagg ccggttcttc cattatgcct gccaaagtca atcccgtgat tcccgaagtg
     1141 gtcaaccaag tctgcttcaa agtcatcggc aacgacacga cgattacctt cgccgccgaa
     1201 gccgggcaac tgcaattaaa cgtgatggag ccggtcatcg cccaatgtat gtttgaaacg
     1261 atttccctct tgggcaacgc cgcagtcaac ctgtccgaca aatgcgtcaa aggcattacg
     1321 gtcaaccgcg aaatctgcga acgttacgtt ttcaactcca tcggtttggt gacttatctg
     1381 aatccgtaca ttggccacca caacggcgac ttggtcggca aaatctgcgc ccaaaccggc
     1441 aaaggcgtgc gcgaggttgt tttggaacgc ggactgttga gcgaagaaga actcaaccgc
     1501 atcctctctc ctgaaaacct gatgaacccc catctgtaac gttgcagaca aatgccgtct
     1561 gaagcagcat ccctgcttca gacggcattt tcggcattca aggctgtttt agccgccgcc
     1621 cgattccggc ggtttgtcgg aaaaagccgg ttatttccgt tcgggcaaaa atctatctta
     1681 ttaataaaaa acaattattt aaaaccattc cttaaagcgc gcccgcgcgg tatgcggaca
     1741 cggcgggatt atatgccgaa cagtgccttg acctccgccc aataaaacag gcaggcgatg
     1801 gaacacagtg ccaatacgat tcccgccgta ttgattgtat tgattttttc cttaaaaaac
     1861 agtgcgcccg aaagcgtacc caaaacaatc acaccgatat tcataccggc aaaaaccaag
     1921 gttggattgt ccttcatcat ttggtgcgcg gtgatgtagg ttacgatatt cataaaattc
     1981 aaaccgccca agagtatgcc gccgaccaca ctctctgcgc gccatctgac cgatttggca
     2041 aacaggcagg caaacatcag cacacccgcc agcacaaatg caaccagcag gttgcccgca
     2101 aatgccgtgc cgcttttggc aatctgtttg aacaggatat cgataatgcc gtaacccgcc
     2161 cacacgccca gcagcaatgc cgcctgccgc cacgcgcttc ctgatttttt gccaccgctg
     2221 tgtttccaaa gcaggcagaa cagtgcggca aatgcgaggc acagcccgat tagtctgcct
     2281 tcgctgagtt tttcgccaaa cagcgtcaag gcggcaacaa tcggcaaaaa cagcgacaaa
     2341 cgctgcgccg cgtcggattt gacgataccg gcggattcta cggatttgcc cataatgaca
     2401 aatacggacg gcagcagcac gcccaatgcg gcaaacagcc accatgtcgg caaaaatgcg
     2461 ccgatattgc cgacatccgg cttcaatacc agcagggtca gtatgaccgc gaccacataa
     2521 ttgacggcga ccgcctgcgc gatgtcgatt tttttcttcc ttgccatttt cagcaaaatg
     2581 gaaaccgaca cgctgaacac gatgctgatt aacaggtaag ccatactata tccatttctc
     2641 gggtgcaacc gccctgccgc cgttgaagcg gataaagccg gcgatgatgc ggtagaaata
     2701 ccagacgttc gttgcaataa taatcagcac gccgataccc aaaagggcag tcagcgcacc
     2761 caaaatataa agccaaaatg tgcgccaaaa ggttttaatc aggtattcgg tatgtgcggc
     2821 atagacaatg ctgtccaaac tgttccgttt catataggcg agaatcacgc cgactatggg
     2881 catataggcg aaaatcacgc cgactatggg catgaacagc atgattgcac ccaaaccgaa
     2941 caggatgtag gcggcaagga catagttgcg cccgcttccg ccgccggacg gttcgtaagt
     3001 atggcacgaa cgtgatgcct gccgttcaaa cgcctgtctg aacgcctgtt cgcggcgcat
     3061 ctgttcgcgg taaaactgct ccctgtgcgc ctgttcgcgg cggaatgcct cttcctgccg
     3121 cccgcgttcc tcatgccgtc tgaaggacgc gtcgtattgc atccgtttcg acaggtcgga
     3181 aagcgtatcg taagcccgtt ggatttcttt gaacttttct tccgccttcg gattgcccgg
     3241 attgcggtcg ggatgatatt tcatcgccag cttgcggtag gcgcgtttga tttcgtccgc
     3301 tcccgcctgc ggcgacacgc ccaatacggc atacaaatct ttgtccatat tgttccttta
     3361 tactttcccg tcaaaccgcc gcctgtattg ttttcgggtt caaaatattt aaatctccgg
     3421 gacggatgcc gataatgaac ccgcgttttc cgccgttgat atagatggtt tccaaatcca
     3481 tcaccgactg ttcgacgtaa atatccaatt ttgtccggat gccgaacggc gttgtgccgc
     3541 cgaccaaata gcccgtccac ttgtttgcct gtgcgggcgt agcgggttcg atgcgtttcg
     3601 cacccaaatg ccgagccaga ttgcgcgtgg aaatctgttt gtcgccgtgc atcaagacaa
     3661 tcagcccctt accgttttca tcttgcaaaa caatggtttt aatgaccaag tgttcgtctt
     3721 tgccgaatag tcgggcaaac tgcgccgtgc cgccgtgttc ctcgtaggga tagatgaaag
     3781 gttcgaactt gatgccgttt tcacgcaaaa cgcgtacggc aggtgttatc ggataagtgt
     3841 gtttactcat ttttcgtcat gcaaaacatg gaaatcggtc aagcgcaatg ttaccatata
     3901 tcccctttat cgaaggaaac cgatatggat atccgatatt tcggcacaac gccccgctat
     3961 tccgaagccg tcggcgcaaa cggcttgatt ttcctctccg gcatggttcc cgaaaacggt
     4021 gaaacggctg ccgaacagac tgccgacgtg cttgcccaaa tcgaccgctg gctggcggaa
     4081 tgcggctcgg acaaagcgca cgttttggat gcggtcatct atctgcgcga tatgggcgac
     4141 tatgcggaaa tgaacggcgt gtgggacgct tgggttgccg ccggcagaac gcccgcccgc
     4201 gcctgcgtgg aagcgcggct cgcccgtccg gagtggcgtg tcgaaatcaa aataaccgcc
     4261 gtgaagaaag atgcggcaac cgcataaaca gatttccttt atatttcaga gtacgcaaat
     4321 gatgaaaacc ttactgccct ttttcggcgt ggtacgcgtc agcggcgaag accgccaaac
     4381 cttcctgcac ggacagcttt ccaacgacat caataatctt cagaccgggc aggcgtgtta
     4441 tgcgacttac aacacgccca aaggccgcgt catcgccaat atgattgtcg tcaaccgagg
     4501 cggcgatttg ctgctgatta tggcgcaaga cttgcttgaa gcaacggtca aacgtttgcg
     4561 gatgtttgtg ttgcgtgcca aagccgtttt tgaaatcctt gaagattacg ccgtcggcgc
     4621 ggaattggaa gcatccgccg aaccgcttgc cgcgcaagaa cccaaccttg cctttgccgc
     4681 acaacaagat tcagacggca tctgctctat cgctctgcct catggcggca tccttcgcat
     4741 cgcccccaaa aacaccttgc cgccttatga tgccgccgcc gaaaacgcgt ggcggctgca
     4801 cgaaatccgt agcggttatc cgtggatatg cgcggctacc aaagaaactg ccgttgccca
     4861 aatgctcaac cagcacatca tcggcggcgt acacttcaaa aaaggctgct atcccggtca
     4921 ggaaatcatt gcccgtgccc agtatcgggg gcaggtcaaa cgcggcttgg cggtattgtc
     4981 gggaaattcg gccgtcgaag caggaaccct gctgacggcg gacggcgaag aggccggcat
     5041 cgtactcgac agcgttcaag attcggaaaa ctttaccgcc cttgccgtga ttaaattctc
     5101 cgccgcacaa aaagaactcg ccgccccaaa cggcggtatc ttcaaagcgg ttcatctctt
     5161 ctttaaaacc gaaaatgcag aataatccct ctgatgccgt ctgaagccct tcagacggca
     5221 ttgccgcccc gtcaatcggt tacaatgccg cctttctaat tttcaaaccc aaaccgccat
     5281 gtccaaaaaa accaaacaag aactcgaaaa caacaaactc agcaaacgcc tgcgccacgc
     5341 cgtcggcgac gcgattaacg atttcaatat gatcgagccg gacgacaaaa tcatggtctg
     5401 cctctccggc ggcaaagaca gctatgccct gttagacatc ctgcgccagc tccaagccag
     5461 cgcgccgatt gatttccaac tggttgccgt caatctcgac caaaagcagc cgggcttccc
     5521 cgaagaagta ttgccgacct atctcgaaag cataggcgtg ccgtacaaaa tcgttgaaga
     5581 agacacctac tccaccgtca aacgcgtatt ggacgaaggc aaaacgactt gttcgctgtg
     5641 cagccgcctg cgccgcggca tcctctaccg cactgcaaaa gaattgggct gcaccaaaat
     5701 cgccttggga caccaccgcg acgacatcct cgccaccttg tttttaaata tgttttacgg
     5761 cggcaaactc aaagccatgc cgcccaagct ggtgagcgac aacggcgaac acatcgtcat
     5821 ccgtccgctg gcgtatgtaa aagaaaaaga cttaatcaaa tacgccgaat taaaacaatt
     5881 ccccatcatc ccgtgcaacc tctgcggctc gcaacccaac ctgcaacgcc aagtcatcgg
     5941 cgatatgctg cgcgattggg acaaacgctt ccccggccgc atcgaatcga tgttctccgc
     6001 cctgcaaaac gtcgtccctt cccaccttgc cgataccgaa ctcttcgact ttgtcggatt
     6061 ggaacgcggt caaaccctga aacacggcgg cgatttggcg tttgacagcg aaaaaatgcc
     6121 cgaacgtttt tcagacggca gcgaagaaga cgaaagcgaa atcaaaatcg agccgcaaaa
     6181 agccgaacgc aaagtcatca acattctggc gaacaagccg aaaacctgcg gcgcgtaaac
     6241 ctccaccgtc aaaaatgccg tctgaagccc gtttggcgtt tcagacggca ttttaaaatc
     6301 ccgaagcaga cggcttccaa acatttgttt ctcagtgtcc gttttcagaa tgcccgcctg
     6361 cgggcatatt ttgcccgatc agttcggcta tcctctcgcc gtcaaactgc gttttgaaca
     6421 ccaccttgat ttctttggaa atctcggcaa acagcttttc ggcatgtttg attttccgct
     6481 cttcgctttt tcgcaaatct tccgccccgt cagtcccttt ggcttcaatc acaaagttca
     6541 gaatctcgcc gctttgggtt ttcacgatat aggcaaaatc gggcgaatac gtgccgccgc
     6601 cggcaacggg gattttgatg gagtttctcg gtattttagt gaatacggtt acgccttcaa
     6661 tttggttgtt ggcgacattt tcatgttcta tatccgaatc gtagaaaatc tcgtcgaaga
     6721 gatagccggc ggcaggccgg tgctccgtat cttcaaatct tcccaaatct gcttttttca
     6781 ccgcgcgcgg tttgccgtct ttatcggtaa atttggtcgg atggattttg ctgccgacaa
     6841 gctggtaatc cagttcgaat ttatggaagg aatgatgaag caaaaaccgg ttgaagccgt
     6901 ttttgatttg ggcgatggtc tgcatattca aaaaatcgcc aatgttcagt tcgttgcgga
     6961 cgcggtaaaa cgcctgatgc aaagtctgca tgcggatttt tgccgtttgt gccagttttt
     7021 ccagaagctc acggtaagtc attgtgttga aacggataaa atcttcatct tcaaagctgt
     7081 ctatgcggcg ggaaagcata agcccgttgt tgatataagc ttcgtttacc gccgtgcgta
     7141 tgcctgcctg cgggaatttg gcggcgtttt cacgcaaata ggcagtaaat aaatcgacaa
     7201 attcggcttc atctttgatt ttgtactgca aaacggcttt atggtgaatc agctcccaca
     7261 aggctttgag ttcttcatat ttgccttcgc gcatgatgat ggtgtctttg ccttcgtctt
     7321 tggcgttgct gactttgcct ttgtccaaac ctttggggaa ggcttcggga taggcggctt
     7381 ttaatttgtc atagccgtct tcggtaaagt tttcattgtc gtcaatgatg ccgtctgaaa
     7441 acagtttgtt taacaatccc agaggtttga tatcggggta tttttgcaat attttttgtt
     7501 tcagctcttc ggtaaacttt tttgagattt cttcctgaaa agaattatcg ttgatttcgc
     7561 cgacaagctg cttcacaaag tctttttcgc tgctatcgac aaaataattc agtttgtacg
     7621 gcacatcgcg cacccgcgcc atcagctcgt ttaccggcag gcgcaggccg cgtccgactt
     7681 cttgcagctt ggaagtcgtg ctgccgctgg aacgcagttt gcaaatctgg aaaacgttgg
     7741 gattgtccca gccttcgcgc agcgtccatt tggaaaaaat aaagcggcgc gggttgtcca
     7801 aagacaacag tttttcctta tcgtgcagga tttcattgat ttcctgctcg attctatcgt
     7861 cgctgtctgt attgtctttg gaaaaataac cgccgtggca ggcggataca tcgtccaacg
     7921 tcttttgcag gtaatcgcgg taaaacgggt cgctttccgt tttcaggcgg cgtgccgctt
     7981 ccgcgtgaat ccagctttca aatttatctt tcaggctgcc tgaaagctcg ttgccgctgc
     8041 ggtagcccgc aatatcatca ataaaaaaca gcgtcagcgg cttgattttg ggctgtggcg
     8101 cgcgttctgc caaaagtgcg cgttccagct tgaaatgttc ggcaaccgcc ctctgcatca
     8161 tcgcatcctg caccgtttgc gaataggaat aagggttgat gacggcgccc gttttcaact
     8221 ccaagccgtt gcttaacacc accacggttt tattcatttt gtcgattttc aaatctgaaa
     8281 cagcaggatg gattttcgcc aaatcttcgc cttttgccag tttgaacgtc tgctttttgt
     8341 ccttttcgtt taattcaaat ttcgcttctt tgccgcccga cgacaccagt tttaccgccg
     8401 catccatgcc gccctgcatt tcttcctgaa acacgcgcac gcctttgacc agcccgtcgt
     8461 taaacgcgtc tactgccgtc aaacggtaaa gcaagttgta atattcatcg ttaaatgtcg
     8521 caccgtagcg caaaatatat tgcggtttta agcgtttgat attgccccat gttttcgcgc
     8581 tctcccgggt cgggaattta tgcggttcgt ccacaatcac aaacgggcgc acggcggcca
     8641 atgcatcaac gggattgtca aacaaatctt tcaatgcctt gtcgcccgta tcgttcatgg
     8701 acgacgaatt aaccatgccc gcgttaatca gcagcacatg gatttccttt ttgttttccg
     8761 ctttggcaaa ttgctcaatc gttatgggcg cattggactt tttgccctta ttctttttcg
     8821 cgctttccac cacataggtt ttcaggcgca cgccttcata atcgccgccg aaatcctgtt
     8881 caaaatgctc tgccaaagcc ttgctttgca aaaactgccg cgttcccgcc ttaatggaca
     8941 aagtcggcac gaccacgata aatttgaaca cgcccagcca acggtgcagc tcgaacatgg
     9001 tttgtgtgta ggtataggtt ttgcccgtgc ccgtttccat ggaaatatca aggatatttt
     9061 ggtcgtccga acggtcgggg aatcggccgt ctatgccgtt ttggctttgc actttcagga
     9121 tattgtctgc gtattgtttt gcagcaaaca aaagttcggg attttcgtct gccgcccgat
     9181 atttgggcgt tgccccgtca aacacgccca aaaccgccga aaccgcccgc atttggtgcg
     9241 gctggttttt ctcgtaatta aaaccactca taaactttct ccgtcaaacc ctgataatca
     9301 cgtttaactc aatttctttt ttattggcat aaccgcgaac cgcctggtca agttcgtgct
     9361 gcatggcgct tgccatattg ctgccgaata caatcacgcg gttgggattg aaatccgcat
     9421 cgtcgtccag cttgcggata aacgccaaca aatcggcgga agtaaaaccg gcattcatca
     9481 gatacagccg tttttcgcac agatacgccg tgtaagcccc taaccgcaca ggctcaaccg
     9541 gcgtggtcag tgccgccccg tcatacagcg tccaggtggt cagaagcgtt tgcagctgtt
     9601 cttcgcttaa ttcatcgtta agcggcaaat ccggttgttc gggcgaaaaa tccttgtccg
     9661 gatgctgcct gaaattgtct gccgtttgaa agattttgaa gcccgaatcg cccgtgtaat
     9721 cgggatgttc gacgcggatt ttggcggcgg ctttttctat gcgggctttg gtgatgtcga
     9781 agatggtcgg gtagcctgct ttacgggctt cggatttttc agcggttttt tcgggaagct
     9841 gtacacagat atagcggcgg ttaccgtttt gtccttcggc gttaagctgc atcacggcgt
     9901 gggcggttgt gccgctgcct gcgaagaagt ctaggattag gtcagtttga tttcccttga
     9961 aagttgcaga aagaactaat cttttcaata aatctaatgg ttttggtgtt tcaaaaggag
    10021 aataaccttt tccagtttca aacaactctt ctaattcacg aataccatct ttagtaagat
    10081 ttttacttat atttccaata tttaagatgc tatgaaattt ggaaaaatca tcggatactt
    10141 ttctaagttt tccaataatt gttttcccat aagacttcgg aattaagata tattcattat
    10201 tttcaatatc tttatcaatt ttgtttttaa actcaattcc ttttatttca ataatatctt
    10261 cgtaatgcaa gttcccatat ttgccaaact gttcgcgaac ggcatcttcc tgtatccaat
    10321 ataattcatc attgattaca atttttttgc cgcgtatatc ttttggtctt tttagattgt
    10381 caaaatgagt aatatctttc gcataaacta aaacagtttc atgcccattt acaacatatt
    10441 ttgcattacc accaccttct gatgtattaa caattaaatc tttaataaaa ttcccttccc
    10501 caaacacttc atcacacaac aacttcaact gcgccgcttc gttatcgtca atcgagataa
    10561 aaatcacgcc gtcgtccttt aacagttcgc gggcgatata caggcgcgga tacataaagg
    10621 tcaaccatgc actgtgcgag tttgagcctt tgtcggtgaa atctaaaatc cgcgcggctt
    10681 cgtcttcgtc aatattggct aagcaggcaa gttcggctgg tgtgaatttg cggtcgtcct
    10741 gatagacaaa gccgtctgat ccggtgttgt agggcgggtc gatgtaaatc atcttcacgc
    10801 tgtttgtgta ggcgtttttt aagtgtttca acacttctag attgtcgcca cgaatcagta
    10861 ggttttggct gcctgcattt tcgggtttgg cgttgtgcgt cttgtcttca cttatcaggg
    10921 tttcgggcgg caggttgcga agcaggcgag catatgattt gcccagccag ttcatttcgt
    10981 aaaattcgcg tccgatgtcg gtctgtggcg caatttcggc ttgtaatctg tcgataagga
    11041 aatttccgtc tgcgtcaaaa caggcgggaa acagtttttt gagctgttcg agttgggtag
    11101 agttggcggt aatgccgtct gaagtgtaga ttgcctcggt gttcaccccg gctgtgtcga
    11161 ttagatcagg g
//
LOCUS       DAXHPG010000054        11160 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXHPG010000054 DAXHPG010000000
VERSION     DAXHPG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11160
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.054"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(64..148)
                     /locus_tag="ACFM6F_001060"
     tRNA            complement(64..148)
                     /locus_tag="ACFM6F_001060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Leu,seq:caa)
     gene            425..1078
                     /locus_tag="ACFM6F_001061"
     CDS             425..1078
                     /locus_tag="ACFM6F_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC6164491.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1157..1810
                     /gene="hisG"
                     /locus_tag="ACFM6F_001062"
     CDS             1157..1810
                     /gene="hisG"
                     /locus_tag="ACFM6F_001062"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC6164492.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1914..2813
                     /locus_tag="ACFM6F_001063"
     CDS             1914..2813
                     /locus_tag="ACFM6F_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC6164493.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2810..4099
                     /gene="hisD"
                     /locus_tag="ACFM6F_001064"
     CDS             2810..4099
                     /gene="hisD"
                     /locus_tag="ACFM6F_001064"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC6164494.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4145..5242
                     /gene="hisC"
                     /locus_tag="ACFM6F_001065"
     CDS             4145..5242
                     /gene="hisC"
                     /locus_tag="ACFM6F_001065"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC6164495.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5239..6156
                     /gene="hisB"
                     /locus_tag="ACFM6F_001066"
     CDS             5239..6156
                     /gene="hisB"
                     /locus_tag="ACFM6F_001066"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC6164496.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6251..7120
                     /locus_tag="ACFM6F_001067"
     CDS             6251..7120
                     /locus_tag="ACFM6F_001067"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC6164497.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            7207..8072
                     /locus_tag="ACFM6F_001068"
                     /pseudo
     CDS             7207..8072
                     /locus_tag="ACFM6F_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
     gene            complement(8123..9175)
                     /gene="sohB"
                     /locus_tag="ACFM6F_001069"
     CDS             complement(8123..9175)
                     /gene="sohB"
                     /locus_tag="ACFM6F_001069"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC6164498.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(9279..9368)
                     /locus_tag="ACFM6F_001070"
     tRNA            complement(9279..9368)
                     /locus_tag="ACFM6F_001070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9332..9334),aa:Leu,seq:taa)
     gene            complement(9471..9544)
                     /locus_tag="ACFM6F_001071"
     tRNA            complement(9471..9544)
                     /locus_tag="ACFM6F_001071"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9510..9512),aa:Cys,seq:gca)
     gene            complement(9571..9646)
                     /locus_tag="ACFM6F_001072"
     tRNA            complement(9571..9646)
                     /locus_tag="ACFM6F_001072"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9611..9613),aa:Gly,seq:gcc)
     gene            complement(9668..9743)
                     /locus_tag="ACFM6F_001073"
     tRNA            complement(9668..9743)
                     /locus_tag="ACFM6F_001073"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9708..9710),aa:Gly,seq:gcc)
     gene            complement(9767..9842)
                     /locus_tag="ACFM6F_001074"
     tRNA            complement(9767..9842)
                     /locus_tag="ACFM6F_001074"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9807..9809),aa:Gly,seq:gcc)
     gene            complement(9868..9943)
                     /locus_tag="ACFM6F_001075"
     tRNA            complement(9868..9943)
                     /locus_tag="ACFM6F_001075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9908..9910),aa:Gly,seq:gcc)
     gene            complement(10023..10586)
                     /gene="pgsA"
                     /locus_tag="ACFM6F_001076"
     CDS             complement(10023..10586)
                     /gene="pgsA"
                     /locus_tag="ACFM6F_001076"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC6164499.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            complement(10692..11115)
                     /locus_tag="ACFM6F_001077"
                     /pseudo
     CDS             complement(10692..11115)
                     /locus_tag="ACFM6F_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2863 a         3162 c         2660 g         2475 t
ORIGIN      
        1 ttagttttat ctatttcatt aaacagcaat agacaaaaaa aataaccgct ctaaaagcgg
       61 ttgtggtgcc cagggtcgga ctcgaaccga cacaccttgc ggcgggggat tttgagtccc
      121 ctgcgtctac caatttcgcc acctgggctg gtgaagaagt cgtcattata atggcttttg
      181 aaattctgta aacctttttt ttgaaattat tttatctgtt tttattttat ttttgatttt
      241 aaatagaatt tttattattt taatcttact gttctttccg ctccaaagat tctgtatgat
      301 tcggcaattc ctgccgtgca gacaacgtaa aaaaatacta cattaaatct gccaaacgcg
      361 ttaagatgga aatattcaaa ttccgtacga atcaggtttt gctatttatt cttgggagat
      421 tgtcatgttt tccgtaccgc gttccttttt gccgggcgtt ttcgtacttg ccgcgcttgc
      481 cgcctgcaaa cctcaagaca acagtgcggc gcaagccgct tcttcaagtg catccgcgtc
      541 ggctgcggaa aatgcggcaa agccgcaaac gcgcggtacg gatatgcgta aggaagacat
      601 cggcggcgat ttcacgctga ccgacggcga aggcaagcct ttcagcctga gcgatttgaa
      661 aggcaaggtc gtgattctgt ctttcggctt tacgcactgt cccgatgtct gcccgacaga
      721 gcttttgacg tacagcgaca cgttgaagca gttgggcggg caggctaagg acgtgaaagt
      781 ggtgttcgtc agcatcgatc cggaacgcga cacgcctgaa atcatcggca agtatgccaa
      841 acagttcaat ccggacttta tcggtctgac ggcaacgggc gaccaaaacc tgccggtcat
      901 caagcagcaa taccgcgtgg tttctgccaa agtcaatcaa aaagacgaca gcgaaaacta
      961 tttggtcgac cactcttccg gtgcgtatct catcgacaaa aacggtgagg ttgccatttt
     1021 ctcgccttac ggaagcgagc cggaaacgat tgctgccgat gtaaggactc tgctctgata
     1081 aaaccgtatg ccgtctgcac cgtcggcgcc tattcagacg gcattattgt ttcaaccgac
     1141 aaaggacatc cacaccatgc aggataatgc tttgacaatc gccttatcca aggggcgcat
     1201 ttttgaggag acgctgccgc tgcttgccgc tgccggcatt gttccgactg aagagcctga
     1261 aaaatcgcgc aagctgatta tcgggacgaa ccatgaaaac atccgccttg tcattgtccg
     1321 cgcaaccgat gtgccgactt atgtccgcta cggcgcggcg gacttcggca ttgcgggcaa
     1381 agacgtgctg atcgaacacg gcggcacggg gctttaccgg cctttggatt tggagattgc
     1441 caagtgccgc atgatggttg ctgtgcgtaa agggtttgat tacgaagcag cttcgcaacc
     1501 cggatgccgt ctgaagattg ccacgaagta tcctgaaatc gcggcatctc attttgccgg
     1561 caagggtgtc catgtggaca ttatcaaact gtacggctcg atggaacttg cgccgctggt
     1621 cggcttgagc gatgcgattg tggacttggt ttcgacgggc aacaccttga aggcaaacgg
     1681 cttggaagca gtcgaacaca tcgtcgacat ttccagccgc ctggtggtca acaaggctgc
     1741 tttgaaaacg aaatacgcgc tgctggagcc gattattcag gcgttcggcg gcgcagtgaa
     1801 ggcgaagtaa gcatccattt gaataaagat gcgttttcag acgaccctat ccgttcccgc
     1861 cgacaggtcg tctgaaaata tcaccggcag taaactgtat aggagaagtt aaaatggttg
     1921 caaaaataaa aaaattctca gattcaaccc tttccgtttt gaataacggc gagcgtcggt
     1981 tttatgtcta ttgtctgacc gacctgaaaa aagacaaaat cctctacatc ggcaaaggct
     2041 gcggtaatcg tatcttcgag catgaatggg ttgctagtcg ttcacaagac ccagtctccg
     2101 gcgagattat cgatcggaaa ctcaaagcca tctccaaatg caagaaactc ggtcgctata
     2161 tcatcagcta tcatctgact gaagtcgaag cactcgccgc cgaatctgcc ttaattcatt
     2221 ttgttaaatc tgtcttgggt aaaaaactca aaaataaaat tgccgggcat ggtccgggtg
     2281 gtattagcgt agaagaacta gatcgccgct ttggattctc ttctctccca cttaacgaga
     2341 ttaaccccga cgggctgatt ctcgccatca aaatccacaa tgctttcgat ttagatactg
     2401 acgaagaatt agactacctt ttcgacaacc aagacgatgc caacctcaaa tcgcgtacgt
     2461 tgggcaactg ggttatcggt aaagatgttg cttcaaaagt gaaatacgtt atcggcgttc
     2521 acaccggtct gcaaaacgct gttgtcagtg catacgaagt ggacggtttt gaaacaatgg
     2581 ttgaggaaac caaaaacggt agaaaacaat cccgttaccg tttccgcact acctctcgta
     2641 gcgaagaggt attagccaaa ctcggtctgc aacaaaaatg cctgcccgaa ttgaagtttg
     2701 gtagcggggg agaaaaagcg tatatcagac ccaaaacaga gacagaaact gaacaagaga
     2761 atattcagac gacccccaat ccaaaaataa aaaaggaaaa aaccaaatca tgaaaaaact
     2821 caacacccaa tcgcccgatt tccaagccgg actcaaagcc ctgctggctt ttgaaaccgc
     2881 gcaaaacccc gaaaccgaac gcatcgtcgc cgacatttgc gccgacgtgc aaaagcgcgg
     2941 cgatgcggct ttgattgaat acaccaacaa attcgatcag acaaacgcta aaagcatcga
     3001 tgatttaata ctcacgcaag ccgatttgaa cgcggcgttc gagcgcattc cgaacgacgt
     3061 tcagacggca ttgcagaccg ccgcccgccg tgtcgaaagc taccaccaac gccaaaaaat
     3121 ggaatcgtgg agctacaccg atgaagacgg cacgctgttg ggacaacaga ttaccccgct
     3181 tgaccgcgtc ggcatttacg tccccggcgg caaggcggca tatccgagtt ccgtcatcat
     3241 gaacgccatg cccgcccacg tcgcaggcgt gaaagaaatc atcatggtcg tgccgacacc
     3301 gaaaggcgaa cgcaacgaca tcgtgcttgc cgccgcatac gtcgccggcg taaccaaagt
     3361 cttcaccgtc ggcggcgcgc aggcggttgc cgccctcgcc tacggcacgg aaaccatccc
     3421 ccaagtcgat aaaatcaccg gtccgggcaa cgccttcgtc gccgccgcca aacgccgcgt
     3481 gttcggcgtg gtcggcatcg acatggtggc ggggccgtct gaaatcctgg tcatcgccga
     3541 cggcacgaca cctgccgatt gggtggcgat ggatttgttc agccaggccg aacacgacga
     3601 aattgcccaa gccatcctca tcggcacgtc gcaagcgtat ctcgacgaag tagaagccgc
     3661 tatggaccgc ctgatcgaaa ctatgccgcg ccgcgacatc atcgaagcct cgctcggcaa
     3721 caggggcgcg atgatactcg ccaaagactt ggacgaagcc tgcgaaatcg ccaactacat
     3781 ttcccccgaa cacttggaac tgtcagtcga aaatccgcag gaatgggcga aaaaaatccg
     3841 ccacgccggt gcgattttca tgggacgcta caccggcgaa agcctcggcg actactgcgc
     3901 cggtccaaac catgtattgc ccaccagccg aaccgcccgc ttttcctcgc ctttggggac
     3961 atatgatttc caaaaacgct ccagcctgat tcaggtttcg gaacagggcg cgcaaaaatt
     4021 aggcgaaacc gccagcgtgc tggcacacgg cgaaagcctg accgcccacg cccgcgcggc
     4081 agagttccgt atgaaataat gccgaaacgg cgtacaggca tattccaacc attaaggaaa
     4141 cacgatgaaa tccgtccgct ccttcatccg cgacgacata caagctatgt cggcatatca
     4201 gattgccgac gttccgcccg gctttgccaa actcgattcg atggaaagtc ccgtccaccc
     4261 ttttgccgga catgaaacgc tgttgcagga atggcaggca cggcttgccg ccgcgcccat
     4321 ccatctttac cccaatccct ccggcagcgg tttacaggaa gcattacgtt cggcgttcga
     4381 cattcccgac tgcgccgaca tcgcgctggg caacggctcg gacgagctga tacagttcat
     4441 cacgatgctg accgccaaac cgggcgcggc aatgttggca gccgaaccca gtttcgtcat
     4501 gtaccgccac aacgccgcgc tgtacggcat ggattatgtc ggcgttccac tgaacggaga
     4561 tttcaccctc aacctgcccg ccgtcctcga agccgtcagg aagcaccgcc ctgccctgac
     4621 ctttatcgcc taccccaaca accccaccgg cgtatgtttc acgcgtgccg aaatcgaagc
     4681 cgtcatcgaa gcttcagacg gcatcgtcgt cgtcgacgaa gcctacggcg cattcaacgg
     4741 cgacagcttc ctgccgcagg cgggcagcat tcccaacctc atcgtcatgc gtaccgtcag
     4801 caaaatcggt tttgccggac tgcgtatcgg ctatgcggca ggctgtcccg aagtcatcgg
     4861 cgaactgcaa aaaatcctgc cgccctacaa tatgaaccaa ctgagcctga ccaccgccaa
     4921 actcgccctg caacactacg gcatcatctc tgccaacatc gacagcctga aaaacgaacg
     4981 cgaacggatg ttcgccgaat tgggcaaaat atgccgtctg aacacctttc caagtcaggc
     5041 aaacttcatt accatacgcg tacccgatgc cgatttgttg tttgacacgc tcaaacaaaa
     5101 ccgcatcttg gttaaaaaac tgcatggcgc gcacccgctt ttggaacact gcctgcgcat
     5161 taccgtaggc agccccgcac aaaacgatgc cgttctcaac atcattcgcc aactttactg
     5221 ccaaccaacg gatttcctat gaatttgact aaaacacaac gccaactgca caactttctg
     5281 accctcgccc aagaagcagg ttcgctgtcc aagctcgcca aactctgcgg ctaccgtacc
     5341 cccgtcgcac tctacaaact caaacaacgc cttgaaaagc aggcagaaga cccagatgca
     5401 cgcggcatcc gtcccagcct gatggcaaaa ctcgaaaaac acaccggcaa acccaaaggc
     5461 tggctcgaca gaaaacaccg cgaacgcact gtccccgaaa ccgccgcaga aagcaccgga
     5521 actgccgaaa cccaaattgc cgaaaccgca tctgctgccg gctgccgcag cgttaccgtc
     5581 aaccgcaata cctgcgaaac ccaaatcacc gtctccatca acctcgacgg cagcggcaaa
     5641 agcaggctgg ataccggcgt acccttcctc gaacacatga tcgatcaaat cgcccgccac
     5701 ggcatgattg acatcgacat cagctgcaaa ggcgacctgc acatcgacga ccaccacacc
     5761 gccgaagaca tcggcatcac actcggacaa gcaatccggc aggcactcgg cgacaaaaaa
     5821 ggcatccgcc gttacggaca ttcctacgtc ccgctcgacg aagccctcag ccgcgtcgtc
     5881 atcgaccttt ccggccgccc cggactcgtg tacaacatcg aatttacccg cgcactaatc
     5941 ggacgtttcg atgtcgattt gtttgaagaa tttttccacg gcatcgtcaa ccacagtatg
     6001 atgaccctgc acatcgacaa cctcagcggc aaaaacgccc accatcaggc ggaaaccgta
     6061 ttcaaagcct tcgggcgcgc cctgcgtatg gcagtcgaac acgacccgcg catggcagga
     6121 cagaccccct cgaccaaagg cacgctgacc gcataaaaaa ccataccgtc tgaaacaccc
     6181 gcaggctttt cagacggtat cggaacagat aagattacac tacactacaa acagaaaagg
     6241 agtaaacatc atgtccgcaa acgaatacac acaaatcggc tggataggct tagggcaaat
     6301 gggtctgcct atggtaacgc ggctcttgga cggcggcatc gaagtcggcg tatacaaccg
     6361 ctcgcccgac aaaactgccc ccatctccgc caaaggcgca aaagtttacg gcaacaccgc
     6421 cgaactcgtc cgcgactatc ccgtcatttt cctgatggtt tccgactatg ccgccgtgtg
     6481 cgacatcctg aacggagtcc gcgacggatt ggccggcaaa atcatcgtca acatgagcac
     6541 catctccccg accgaaaacc tcgccgtcaa agcacttgtc gaagccgcag gcggacagtt
     6601 tgccgaagca cccgtttccg gatcggtcgg gcccgccacc aacggcacgc tgctgattct
     6661 gttcggcggc agcgaagccg ttttaaaccc gctgcaaaaa atattttccc tcgtcggcaa
     6721 aaaaaccttc catttcggcg atgtcggcaa aggttcgggc gcgaaactcg tcttgaactc
     6781 gctcttgggc attttcggcg aagcgtacag cgaagcgatg ctgatggcgc ggcagttcgg
     6841 catcgatacc gacaccatcg tcgaagccat cggcggctcg gcaatggact cgcccatgtt
     6901 ccaaaccaaa aaatccctgt gggcaaaccg cgaattccca cccgccttcg ccctcaaaca
     6961 cgcctccaaa gacctcaacc tcgccgtcaa agagcttgaa caggcaggca acaccctgcc
     7021 cgccgtcgaa accgttgctg ccagctaccg caaagcagtc gaagccggct acggcgaaca
     7081 ggacgtttcc ggcgtttacc tgaaattggc agaacactga ttgccttttc caaacacaat
     7141 gccgtctgaa catatttcag acggcatttt tatcacccca cgctaaataa aaaataggga
     7201 acaaatatgt ggaaaatgtt gaaacacata gcccaaaccc accgcaagcg attgattggc
     7261 acattttccc tggtcggact ggaaaacctt ttgatgctgg tgtatccggt gtttggcggc
     7321 cgggcgatca atgccgtgat tgcggggggg tgtggcaggc gttgctgtac gctttggttg
     7381 tgcttttgat gtggctggtc ggtgcggcgc ggcggattgc cgatacgcgc acgtttacgc
     7441 ggatttatac cgaaatcgcc gtgccggtcg tgttggaaca gcggcagcga caagtcccgc
     7501 attcggcggt aactgcgcgg gttgccctgt cgcgtgagtt tgtcagcttt tttgaagaac
     7561 acctgccgat tgccgcgaca tccgtcgtat ccatattcgg cgcgtgcatc atgctgctgg
     7621 tgctggaatt ttgggtcggc gtgtcggcgg tgggcatact tgcgttgttt ttatggcttt
     7681 tgccacgttt tgccgccatc agcgaaaacc tgtatttccg cctgaagaac agcttggaat
     7741 gcgacaacca ctttatccga aaaggagacc ggcggcagct ggaccgccat tacggactgg
     7801 ttgcgcgcct gcgtgtgctg atttccaacc gcgaagcctt cggctatctc tgcgtcggcg
     7861 cggcgatggg tattttgttc ggctttgctt ttgtgatgat gacgctcaaa ggctacagca
     7921 gcgcggggca tgtctattcg gtcggcactt atctgtggat gtttgccatg agtttggacg
     7981 acgtgccgcg attggtcgaa caatattcca atttgaaaga catcggacaa cggatagagt
     8041 ggtcggaacg gaacatcaaa gccggaactt gaaaaatgcc gtctgaacac gcttcagacg
     8101 gcatttccat ccgttcggca aactacatca catccgcccg ccggttgaca agtttggcaa
     8161 acaacttttc aacagaagct tccgcctgca aaccaatgcg ctggatcagg ctttgcttct
     8221 cctgatattt cacttcgata acctgtttgt tttcaaacgc tttcaacaac aaatcatcac
     8281 tggtcgaaat ctcgtcaatc aagttcaacg ccaacgcctg ccgaccgaac caatgctcgc
     8341 ccgttgccac ttcctcaata tccaattgag ggcggttctc gctgacaaac tgcttgaaca
     8401 actgatgcgt ttcctccagt tcctgtcgga atttctgttt gcccttttcc gtattttcac
     8461 ccataaaagt aaccgtgcgc ttaaattcgc ccgccgtcat cacatccaca tcaatatcat
     8521 gttttttcaa caggcggtgg atattcggta cttccgccac cacacccacc gaaccgacaa
     8581 tcgcaaacgg agcggaaaca attttatccg ccacacacgc catcatataa ccaccgctcg
     8641 ctgccacctt atcgacggcg acggtcagcg gaatattgcg ttcgcgcaaa cgcctaagct
     8701 gtgaagccgc caaaccgtaa ccgtgaacca cgccgcccgg actttccaat ctaagcagaa
     8761 cctcatcttc aggcttggca atcaaaagca ccgccgtaat ctcatgacgc aaggattcta
     8821 cggcgtgtgc atacaaatcg ccgtcaaaat ccaacacaaa aaggcgggat ttttgcgttt
     8881 cggaagattt ctccccaccc tccttcaaac gctttttctc tgctttggct tccgcctttt
     8941 cctttttctt ttcctctttt tcctgatgtt ttgcctcttc cccgcttaaa aagaatgctt
     9001 caaacgattg ccgctgtttt ttataatttt ccgaaaaatc cgtcagtacg acactgccgc
     9061 tttccgactg tttcttactc tgtacgatag ccaacacaat cagcgcaatt gcgccgaaca
     9121 cggtaagcag ttcgagcagg aaaataccgt aattcagtaa aatttctttc cacattgatt
     9181 ggatttcctc ttgttcaggc atgaacatgt caatattgtc catcaccgtc cgacagataa
     9241 aaaaataacc gcttggagcg gcattgtcat tttcagcttg gtgcccggag ccggaatcga
     9301 accggcacgg gatgtttagt cccgacggat tttaagtccg ttgtgtctac ctatttcacc
     9361 acccgggcat ttgtgaaagg tggaggcggg ggcgcggatt ttaaccggcc tatatgaaga
     9421 ttgcactcct catagcataa acactctgcc accccgccat agtacgataa tggaggcgag
     9481 agtcggaatc gaaccggcgt agacggattt gcaatccgct gcataaccac tttgctatct
     9541 cgccctaaaa ctggcttatc taaaaaaact tggagcggga aacgagtctc gaactcgcga
     9601 cctcaacctt ggcaaggttg cgctctacca actgagctat tcccgcgcgt tcaaacatat
     9661 cggtttttgg agcggaaaac gagtctcgaa ctcgcgacct caaccttggc aaggttgcgc
     9721 tctaccaact gagctattcc cgcgttgata tgtttgaaat aaaacttgga gcggaaaacg
     9781 agtctcgaac tcgcgacctc aaccttggca aggttgcgct ctaccaactg agctattccc
     9841 gcaatgattg tgggaaaatg aaatttttgg agcgggaaac gagtctcgaa ctcgcgacct
     9901 caaccttggc aaggttgcgc tctaccaact gagctattcc cgcccgattt cattctccaa
     9961 catcgaagag acgcaattat tatggattct gtttttgccg tcaagctatt tttatgtttt
    10021 tttcaggcga tttctttcca cgccattttc agataataca gcatcgacca gactgtcagc
    10081 aaagatgcga taaacatcaa tacattgccg ataacaacaa gattaaatcc gtaaaaatcg
    10141 ggaatattca gcagcagcag gaaaattgcc agcatttgcg cggcggtttt aaacttaccg
    10201 acggtggcga cggcaacgct gttccttttg cccatttgcg ccatccattc gcgcaatgcg
    10261 gaaatggtaa tttccctgcc gataatgatc atggcaaaca aaacataggt ccggtcgagt
    10321 ttgaccagta aaagtagcga tacggcaacc atcagcttgt cggcgacggg atcgaggaag
    10381 gcgccgaaat cggaagtctg tttccacagt cttgccaaaa atccgtcaaa ccagtcggtt
    10441 aaggcggcaa cggcaaaaat gacggcggcg gtgagattaa tcgtttcctc cgcgaaccac
    10501 ggaaaaggca ggtaaaaaag ggctgtcatg acaggaatga gcaagaccct caaccatgtg
    10561 aggaagatgg ggagattcca aggcatcggt tttctctgtg cagactgtaa agttgtgatt
    10621 ataacggtta tcctcataac ccaaaacgta aaattgctgc atgggcattc ccccgccccg
    10681 ccaatctgtt ttcacattct tttcaaacgc aggaaaatgg cgggcaataa aagcaaaata
    10741 cccagtttca ggctgaaaac ggcaggttgt gccaacactt cgacaaggcg gtcttccgtg
    10801 cgggcaaaat ctttattgct tatagacact gccactgttg cggtattcca acagaacgcc
    10861 gtttaaaaaa cctttgccga cggtttcgct taaaacggct ctaacctgct ccgccctgat
    10921 ggttctgccg atattgcctt ccgcgcacaa accgtcgaac ccatagcagg aaagccggta
    10981 atgctgcccg tctgcatcca gtttgattgc ccgtccgctg cggttggggg cggtaacggt
    11041 caattccgca tattcgaatg ttttttcttg ttcgtgaaat gccgtcaggt aaggtgcaat
    11101 aaaaacggcg gacaacagca gacagcttat ggcggcaaac catacccagc gataatatag
//
LOCUS       DAXHPG010000055        11075 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXHPG010000055 DAXHPG010000000
VERSION     DAXHPG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11075
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.055"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(68..1624)
                     /gene="dnaA"
                     /locus_tag="ACFM6F_001078"
     CDS             complement(68..1624)
                     /gene="dnaA"
                     /locus_tag="ACFM6F_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC6164500.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRHTAEPASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            1917..2051
                     /gene="rpmH"
                     /locus_tag="ACFM6F_001079"
     CDS             1917..2051
                     /gene="rpmH"
                     /locus_tag="ACFM6F_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC6164501.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            2054..2419
                     /gene="rnpA"
                     /locus_tag="ACFM6F_001080"
     CDS             2054..2419
                     /gene="rnpA"
                     /locus_tag="ACFM6F_001080"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC6164502.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            2485..2706
                     /gene="yidD"
                     /locus_tag="ACFM6F_001081"
     CDS             2485..2706
                     /gene="yidD"
                     /locus_tag="ACFM6F_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC6164503.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            2878..4515
                     /gene="yidC"
                     /locus_tag="ACFM6F_001082"
     CDS             2878..4515
                     /gene="yidC"
                     /locus_tag="ACFM6F_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC6164504.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQTAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKSEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(4596..5321)
                     /locus_tag="ACFM6F_001083"
     CDS             complement(4596..5321)
                     /locus_tag="ACFM6F_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC6164505.1"
                     /translation="MSEGMMSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAE
                     KTARAHLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVA
                     DPGANLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLK
                     ALEQRSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCTATDLTLPTQEIISQTVV
                     QWRKRKEMPNLKKRPTIFVMYAG"
     gene            complement(5368..5958)
                     /locus_tag="ACFM6F_001084"
     CDS             complement(5368..5958)
                     /locus_tag="ACFM6F_001084"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC6164506.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPKALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            6106..6609
                     /locus_tag="ACFM6F_001085"
     CDS             6106..6609
                     /locus_tag="ACFM6F_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC6164507.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            6643..6822
                     /gene="rpmF"
                     /locus_tag="ACFM6F_001086"
     CDS             6643..6822
                     /gene="rpmF"
                     /locus_tag="ACFM6F_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC6164508.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            6997..7524
                     /locus_tag="ACFM6F_001087"
     CDS             6997..7524
                     /locus_tag="ACFM6F_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC6164509.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            7612..8667
                     /gene="plsX"
                     /locus_tag="ACFM6F_001088"
     CDS             7612..8667
                     /gene="plsX"
                     /locus_tag="ACFM6F_001088"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC6164510.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            8803..9054
                     /locus_tag="ACFM6F_001089"
     CDS             8803..9054
                     /locus_tag="ACFM6F_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC6164511.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            9081..9497
                     /locus_tag="ACFM6F_001090"
     CDS             9081..9497
                     /locus_tag="ACFM6F_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164512.1"
                     /translation="MKTSTVVFGGFFMADNRERIQIPVLENPGIREINHFFSVSNFEK
                     KAGVLVFRIIPEPKFGNTELTIYFEEGCYLPMIQTILEDGDIEVKNLKTENYSGNTME
                     IWGDSYPIEHISKNISTIQNIISEFIIQKQTPAPII"
     gene            9517..9720
                     /locus_tag="ACFM6F_001091"
     CDS             9517..9720
                     /locus_tag="ACFM6F_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164513.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPAPPVHGNLHLKTICIPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            9747..10709
                     /locus_tag="ACFM6F_001092"
     CDS             9747..10709
                     /locus_tag="ACFM6F_001092"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC6164514.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            10756..>11075
                     /locus_tag="ACFM6F_001093"
     CDS             10756..>11075
                     /locus_tag="ACFM6F_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC6164515.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLIL"
BASE COUNT         2865 a         3290 c         2554 g         2366 t
ORIGIN      
        1 ttaatccgct atatctttac ccttcggacg gcatgggcaa tatcatgtcg tctgaaaacg
       61 ttttccatca gttttgaatc agaatcagca gcttttcata atcctgagcc aattccggat
      121 cttcttcgcg cagtttcgcc actgccctga tgccgtgcat aacggtcgta tggtcgcgtc
      181 cgccgaacga atcgccgata gacggcaggc tcaaagtggt cagttctttg gtcaggctca
      241 tcgccacctg gcgcggacgg gcaatgtttc gtgtccgttt cttaccgagc acatcgctga
      301 ttttgatgcg gtaatatttc gcaacggcat caataataat atcggcggtg atgactttgt
      361 gcttctcggc aataatgtcc tgcaaagcag tacgcgccaa atcgatgtcg atgacgggac
      421 ggttcataaa gcggctgctc gctccgacgc ggttgaacgc gccttcaagc tcacgcacgt
      481 tggaacggat cagattggca atgaacagcg cggcttcgtc ttcaatactg atgcccgccg
      541 cttccgcctt tttctgcaaa atggcgatac gcatttccaa ttcgggcggc tcgagttcca
      601 aagtcagtcc ccatgaaaaa cgggatttga ggcggtcgtc cataccttcg attttggcgg
      661 gtaaaacatc gcaagtgagg atgagctgtt ttttctcgtt atggaaatga ttgtacagat
      721 agaagaattc ctccatcgta cggtctttgc ctttgatgaa ctggatgtcg tcgataatca
      781 gcaggtcgta ttgcttgtat tgctgcttga acacgtcgta ggtattgttg cgaaccgcct
      841 tcataaagct gcggatatag tcgtccgaat gcatatagcg cactttggca tcgggacggt
      901 ttttcaaaag ctcgttgccg accgcctgta ccaggtgggt tttgcccaaa cccgtgctgc
      961 cgtacaggaa aaacgggttg tagctctgcc ccgggctttc cgcaatcgcc tgcgccgcag
     1021 ccgccgcaag gcggttgccc ttgccttcga ccaatgtgtc gaacgtatag tccggcgaca
     1081 gattggtctg ctcgtaacgt gcctcttccg catcgcgctg cgcatccgtc cgcgctttcg
     1141 caagcacctc cgattccgga cgggaagcag gttcggcagt atggcgcggc tcatgcggga
     1201 ggtttttcat acgttccgcc agaatatccg ccgccgtttt cgacgcagcg ggtttgacag
     1261 gctcttcaga cggcagctcg tccaacagaa cctcctgcac aggcatctcc tccgaccccg
     1321 cgtgcaagga cggctcggca ggttcgacag caccttcaac cgccgccatc tcataacgca
     1381 cgccttctcc tggtttgaat acgaaggcgg aacggccagc agccaactct tccctcaccg
     1441 cttctatttt cccggcaaac tggctcttaa gcatattgca ggcaaactgg ttcttgccgt
     1501 acaccaccca tacgccaccc tcctcaccga cggtaagggg cgcaatccat tgcgcaaact
     1561 gcccttgagg caacatatcg tgaagacggc ggaggcacag cggccaaaac tctgctaatg
     1621 tcatggataa gctcgaatcg gtaaaaatga aatcgaaaac aaagaaaata taatattttc
     1681 aaaaagaaaa caaatctgtt gaacacacat cggttcaaaa tgcagcgaac cgaattatac
     1741 cgactcgccg atattttatc cacaacccgt gcaaaattta tccacagaaa ggcggcagaa
     1801 atccgcaggc aatcgggcaa tcctcctgca aagtttctat attgattgac aaaagcggca
     1861 aattggagtg taattcacgg tttaattatc tacccattct atttttagga aacatcatga
     1921 aacgcactta tcaaccttcc gttaccaaac gcaaacgcac ccacggcttc ctggtgcgct
     1981 ccaaaacacg cggcggccgc gcagtattgg ctgcacgccg cgccaaaggc cgcaaacgcc
     2041 tggcggtata attttggact accgcttcgg aaggcagtac cgcttgttga aaacggatga
     2101 tttttcatcc gtttttgcat tcagaaaccg ccgcagccgc gacctgctgc aagtttcgcg
     2161 ttcaaacggc aacgggctgg accatccccg catcggtctg gtggtcggca aaaaaaccgc
     2221 caaacgcgcc aacgaacgaa attatatgaa gcgcgtcatc cgcgactggt tcagattgaa
     2281 caaaaaccgg ctgccgccgc aggatttcgt cgtgcgcgtc cgccgtaaat tcgacagggc
     2341 taccgcaaaa caggcaaggg cggaactggc acaactcatg ttcggcaacc ccgcaaccgg
     2401 atgcaggaaa caggcatgat cagaacggta ctctgcaggc aaggttcaga cggcaacggg
     2461 tttcccatac taaggaacat cccgatgaac ttcctattgt ccaaactcct gctgggactg
     2521 atacggttct accaatattg catcagcccg ctgattccgc cgcgctgccg ttatacgccg
     2581 acctgttcgc aatacgcggt cgaagcggtc aaaaaatacg gcgcattcaa aggcggccgg
     2641 ctcgccatca agcgcattgc acgctgccac cctttcggcg gacacggaca cgaccccgtt
     2701 ccctgacccg acgcaatatt caaattgcac gctttccttt tatttcccat cggtttctat
     2761 ataatgccgt ctgaactttc gggctggcgg cacgaccgtc gggtatgaag cccgccctta
     2821 ttccccgtct atcggaacac gcaacctgcg gcatttccga ccattcagga aactcttatg
     2881 gattttaaaa gactcacggc gtttttcgcc atcgcactgg tgattatgat cggatgggaa
     2941 aagatgttcc ccactccgaa gcccgtcccc gcgccccaac agacggcaca acaacaggcc
     3001 gtaaccgctt ccgccgaagc cgcgctcgcg cccgcaacgc cgattaccgt aacgaccgac
     3061 acggttcaag ccgtcattga tgaaaaaagc ggcgacctgc gccggctgac cctgctcaaa
     3121 tacaaagcaa ccggcgacga aaataaaccg ttcatcctgt ttggcgacgg caaagaatac
     3181 acctacgtcg cccaatccga acttttggac gcgcagggca acaacattct aaaaggcatc
     3241 ggctttagcg caccgaaaaa acagtacagc ttggaaggcg acaaagttga agtccgcctg
     3301 agcgcacctg aaacacgcgg tctgaaaatc gacaaagttt atactttcac caaaggcagc
     3361 tatctggtca acgtccgctt cgacatcgcc aacggcagcg gtcaaaccgc caacctgagc
     3421 gcggactacc gcatcgtccg cgaccacagc gaacccgagg gtcaaggcta ctttacccac
     3481 tcttacgtcg gccctgttgt ttatacccct gaaggcaact tccaaaaagt cagcttctcc
     3541 gacttggacg acgatgccaa atccggcaaa tccgaggccg aatacatccg caaaaccccg
     3601 accggctggc tcggcatgat tgaacaccac ttcatgtcca cctggatcct ccaacccaaa
     3661 ggcggacaaa gcgtttgcgc cgctggcgac tgccgtatcg acatcaaacg ccgcaacgac
     3721 aagctgtaca gcaccagcgt cagcgtgcct ttagccgcta tccaaaacgg tgcgaaatcc
     3781 gaagcctcca tcaacctcta cgccggccca cagaccacat ccgttatcgc aaacatcgcc
     3841 gacaacctgc aactggccaa agactacggc aaagtacact ggttcgcctc ccccctcttt
     3901 tggcttttga accaactgca caacatcatc ggcaactggg gctgggcgat tatcgtttta
     3961 accatcatcg tcaaagccgt actgtatcca ttgaccaacg cctcttaccg ttcgatggcg
     4021 aaaatgcgtg ccgccgcgcc caaactgcaa gccatcaaag agaaatacgg cgacgaccgt
     4081 atggcgcagc aacaagccat gatgcagctt tacacagacg agaaaatcaa cccgctgggc
     4141 ggctgcctgc ctatgctgtt gcaaatcccc gtcttcatcg gattgtattg ggcattgttc
     4201 gcctccgtag aattgcgcca ggcaccttgg ctgggttgga ttaccgacct cagccgcgcc
     4261 gacccctact acatcctgcc catcattatg gcggcaacga tgttcgccca aacctatctg
     4321 aacccgccgc cgaccgaccc gatgcaggcg aaaatgatga aaatcatgcc tttggttttc
     4381 tccgtcatgt tcttcttctt ccctgccggt ctggtattgt actgggtggt caacaacctc
     4441 ctgaccatcg cccagcaatg gcacatcaac cgcagcatcg aaaaacaacg cgcccaaggc
     4501 gaagtcgttt cctaaatgcc gcagcatgaa aaatgccgtc tgaaacctgt tcagacggca
     4561 tttttattgt tcacccccta tcggggcgga aatcttcaac ctgcatacat cacaaaaatc
     4621 gtcgggcgtt ttttcagatt gggcatttct tttctttttc gccactgcac aaccgtctgg
     4681 ctgatgattt cctgagtcgg caacgtcaaa tccgtagccg tacacaaacg cgtttcagaa
     4741 tgcaggtttt ccaccgcatc cgccaacaac acatcgttgc gatacggcgt ttcgataaac
     4801 agctgcgttt cattctgctg gcgcgaacgt tgctccaaag ccttcaaact ctgaatccgc
     4861 tcgttttttt cagacggcag ataaccctta aacgcaaaac tctgcccgtt cgcacccgaa
     4921 gccatcaaag ccagcagcag gctggaaggc ccgaccagcg gacgcacttc aaaaccgtgt
     4981 ttatgcgcca aagccaccag attcgcaccc ggatcggcaa cagccgggca acccgcctcg
     5041 ctgacaatgc ccatactgcg cccttcttgc aaaggtttca gcaattccag caaagtcttc
     5101 aaatccgtgt gttcgttcag cgtttgcaaa ttcagttcgc ggataggcat tgtcacgccc
     5161 aaatgtttca aatgcgcgcg cgccgttttt tccgcttcca cgacaaaatc cgtcagcccg
     5221 acaatcgcct gttgttcatg cggcaacagg cacggcgtat cgggcgtacc caaaggcgta
     5281 ggaatcaaat acaaaacagg agacatcatt ccctcactca tcggttaaaa atgccgtctg
     5341 aacccttcag acggcataaa cgggcagtta caaaacatcc acgccctcat ttttcaaaaa
     5401 atcgaccaga cggaaaaccg gcaaaccgat taaagcattc ggatcggtac tctcaatcct
     5461 ttcaatcaac aatgcaccca aatcctcact cttcagcgca cacgaacaat aaaccgcatc
     5521 aggctcgcgc tccaaatagc ggaggatgtg caactcgtcc aactgcctca tcacgaccac
     5581 cgtcttatcg atatgccgct gcatcctgcc cgtaaccgta tttagcagaa cgaccgcgct
     5641 gtaaaactca atctccctgc cgctcaaatg catcaacatc ttttgcgcat tagcaaggtt
     5701 catcggcttg ccccactgcc tgccgtcgca ccacgccacc tggtccgcac cgacaatcaa
     5761 cgcctttggg aaacgcccgg tcaacgaccg cgccttaccc tcggcaaggc gcaatgccgt
     5821 ctgaggggcg gattccccca acatcggcgt ttcgtcaaaa tcgggggacg ccgcctgaaa
     5881 ggcaatgccg agcctttcca tctgttcgcg gcggaaaacc gaactcgtac ccaaaatcaa
     5941 aggcagttcc aaacccatcc catcctcctt accgttgaaa acacgcccga aggggcggta
     6001 aaatccagcc atgcgccgaa acacggatac ccgccttcgg cgtaccgcaa catttttctt
     6061 aaaaatattg acgttagaac atctaaatta tatcataccc cgtttatgtc agaccctaat
     6121 ttgattgacc cggaaatttt cgccgccgaa gggcagaatc tgcaaggcag ttttctgctg
     6181 gaagaattgg atgaacgcgt cagttcgcac gattatcccg ccgacaggca gaccaaaata
     6241 tcgtttacac tgaccggcgg tcgcgaccgg ctgcaacgcc tgttcctcga cctgaacgtc
     6301 aaagccgata tgcccctgat ttgccagaga tgtatcaaac ccatgccgtt catgcttgat
     6361 gaaagcagcc gtatcgtcct gttttccaac gaagagtcct tggacgaatc catgcttgcc
     6421 gacgaagaac tcgaaggcat actgattgaa aaagaactcg acgtgcgcac attggtagaa
     6481 gaccaaatcc tgatgtccct gcccttttcg ccgcgacacg aagactgcgg cgacaatggg
     6541 acactggaag aagtcaatcg ggacaaaccc aacccctttg ctgttttggc aggtttgaaa
     6601 agcaattgat taggacacag tttatttatc taggagcttg aaatggccgt tcaacaaaac
     6661 aaaaaatccc cttccaaacg cggtatgcac cgttcgcacg acgcgctgac cgcgcctgca
     6721 ctgtctgtcg acagcacaac cggcgaagta caccgcccgc accacatctc ccccaacggt
     6781 atgtaccgcg gccgcaaagt ggtcaaagcc aaaggcgaat aatccctatt cgactgactg
     6841 aaaaagccag aacattgcca tgcaattact ggcttttttt gcattggacg caccatccgt
     6901 ccaaactttc gccatacgtc aacacacagg ggcaaagcgt tccgtataat accccgtgaa
     6961 aatattccaa aagccccaac caccaaggaa attccgatga aacagaaaat ctggtacacc
     7021 tacgatgaca tccaccgcgt catcaaagca ttggcagaaa aaatccggaa cgccgacatc
     7081 aaatacgatg ccatgattgc catcggcggc ggcggcttta ttccggcacg tatgctgcgc
     7141 tgttttctgg aaattccgat ttatgccgta accaccgcct attacgacag cgacaacgaa
     7201 ggacaggtta cagaagaagt caaaaaagtc caatggctcg accccgttcc cgaagccctg
     7261 cggggcaaaa acgtccttgt tgtcgatgaa gtggacgaca gccgcgtaac catggaattt
     7321 tgcctgacag agctgctcaa agaagacttc ggtacgatcg gcgtcgccgt actgcacgaa
     7381 aaaatcaaag ccaaagcagg caaaatcccc gaaggcattc cctatttcag cggcatcacc
     7441 gtagaagact ggtggatcaa ctatccgtgg gacgcactcg acatcgacga acacaaccgc
     7501 cttgccgagg ccggccgagg ctgacccttt cagacggcat attttccgaa ccgatgccgt
     7561 ctgaagcccg cacgacccct gccgcagacc gaaaacctac cggagaaccc tatgattaca
     7621 cttgccgtag atgccatggg cggcgaccaa ggacttgccg ttaccgtacc cggcgcaacc
     7681 gcattcctcc aagcacaccc cgatgtccgc ctgattatga ccggcgacga aacgcaactg
     7741 cgccaagccc tgaccgcggc aggcgcaccg atggaacgca tcgacatctg ccataccacc
     7801 caagttgtcg gcatggatga atcccctcag tccgccctaa agaacaaaaa agactcctcc
     7861 atgcgcgttg ccatcaacca agttaaagaa ggcaaagccc aagccgccgt atccgcaggc
     7921 aacacgggcg cactcatggc aaccgcacgt ttcgtcctca agaccatacc cggtatcgaa
     7981 cgccccgcca tcgccaaatt cctcccgtcc gacaccgacc atgttaccct cgccctcgac
     8041 cttggcgcga acgtcgactg cacgcccgaa cagctcgccc aatttgccgt tatcggcagc
     8101 gaactcgtcc acgcactcca tcctcaaaaa ggacagccgc gcgtcgggct ggtcaacgtc
     8161 ggcacggaag acatcaaagg tacggacacc gtcaaacaaa cctacaaact gctgcaaaac
     8221 agcaaactca actttatcgg caacatcgaa agcaacggca tcctgtacgg cgaagcagat
     8281 gtcgtcgtcg ccgacggctt tgtcggcaac gtcatgctca aaaccatcga aggcgcggtc
     8341 aaattcatga gcggagccat ccgccgcgaa ttccaaagca acctgttcaa caaacttgcc
     8401 gccgttgccg ccctacccgc cctcaaaggg ctgaaaaaca aactcgatcc gcgcaaattc
     8461 aacggggcca tcctgctcgg gctgcgcggc atcgtcatca aaagccacgg cggcacagac
     8521 gaaaccggtt tccgctacgc actcgaagaa gcctaccacg aagccaagtc cgccggcctt
     8581 tccaaaatcg aacaaggcgt tgccgaacaa ctcgccgcac tcgaaactgc caaagccgtc
     8641 caaaacgaaa atgtcggcgg tctgtaacac acacgatgcc gtctgaacgc ccccgcccct
     8701 ttcagacggc atccgcccgc cccgaacctg cgggcgcgga cggcgatgca cctgtccggc
     8761 acttcccaaa tacttccctg taaaataagg aatatttgaa aaatgaagac attagaaaaa
     8821 cggatgaaag ctctagacaa acggatgatg aagttcggaa aatcccttga aggcaggctt
     8881 gatgcccgtc tgattgaatc cgcattggat tatattcatt attcggaacg ttttttggct
     8941 tttgaaatcc tgtgtactta tatcgaagat ttcgatgtcc ggctgacgga acaagaatcc
     9001 cgggaaattt cttttatcaa caaggaattt gagatagaaa gcacgtccga ttaaccaata
     9061 aagccaatga tttgataaga atgaaaacat cgacggtcgt ttttggcgga ttttttatgg
     9121 cagacaacag ggagcgaatc caaatccccg ttttggaaaa tcctggcatt agggaaatca
     9181 atcacttttt ttccgtatca aattttgaga aaaaagccgg cgtccttgtt ttcagaatta
     9241 ttcctgaacc caaatttggc aataccgaat taactatcta ttttgaagaa ggatgttatt
     9301 tgccaatgat tcaaacaatt ttagaagatg gggatattga agtcaaaaac cttaaaacag
     9361 aaaattatag tggaaataca atggaaattt ggggagattc ctaccctatt gagcatatct
     9421 ctaaaaatat ttctacaatt caaaatataa tttctgaatt tattatacaa aaacaaacac
     9481 cggcaccaat aatttgatgg aaaaccaaca atcaaaatgc cgtctgaagc atgtttggtt
     9541 ttcagacggc atttccttcg cttaaaacag cgtatcggca accccgcccc gcccgtccac
     9601 ggcaatctgc atctgaaaac catctgtatc ccaaaccaca cccccatccc tgtttccgtc
     9661 atttgcaccc tgcccgtatt gggcaatcat cggtttttcg cttacaatag ccgaatctga
     9721 accaactctc taaaaaggcc gttcccatgc agtatgcaaa aatttccggc acgggcagct
     9781 atcttcccgc caaccgcgtc agcaatgacg accttgccca aaaggtagat acctctgacg
     9841 agtggattac cgcgcgtacg ggcatcaagt tccgccatat tgcagccgaa aatgagaaaa
     9901 ccagcgatct tgccgccgaa gcagcccgcc gcgcactgga tgcagccgga ttagacagcg
     9961 gcgaaatcga tttaattatt gtggcgacgg caacgccgga tatgcagttt ccttctaccg
    10021 ccaccatcgt ccaacaaaaa ctgggcatca ccaacggctg ccccgcgttt gacgtacagg
    10081 cggtgtgtgc gggctttatg tacgcgctga ccacggcaaa cgcctacatc aaaagcggca
    10141 tggcaaaaaa cgcgctggtc atcggcgcgg aaaccttcag tcgcattgta gactggaacg
    10201 accgcacaac ctgcgtattg ttcggcgacg gtgcgggcgc ggtggtttta agcgcgtccg
    10261 acaaaccggg catcatccac agcaaactca aagccgacgg caattatctg aaactcttaa
    10321 acgtccccgg gcaaatcgcc tgcggcaaag tttccggttc gccgtacatt tcgatggacg
    10381 gtcccggcgt gttcaagttt gccgtcaaaa tgctgtccaa aatcgccgat gacgttatcg
    10441 aagaagccgg ttataccgcc gctcaaatcg actggatcgt gccgcatcag gcaaaccgcc
    10501 gcattatcga atcgaccgcg aaacatttag gtttgagtat ggacaaagtc gtcctgaccg
    10561 tccaagacca cggcaacaca tccgccgcat cgattccgct ggctttggat acgggcatcc
    10621 gcagcggaca aatcaaacgc ggtcaaaacc tgctgctcga aggcatcggc ggcggtttcg
    10681 cgtggggcgc ggtgctgttg caatattgaa cccgatgccg tctgaaacag gctttcagac
    10741 ggcatttccc atatcatgaa gcggcaggct ttcttcaaac tgatggcgtg tgcggcattt
    10801 ctgtctgccg tttcgctgcg cctccccgta ttgggcgcgt gttacgcaat attgtccctc
    10861 tatgcgtttg cactttacgg catcgacaaa cggcgtgccg tgcggggaaa acgccgcatt
    10921 cccgaacacc gcctgctgct gcctgccttg ttcggcggtt gggcgggcgc atacttgggc
    10981 agcaggatat tcaggcataa aacggcgaaa aagcgttttg ttgtgctgtt ccgtctgact
    11041 gtttcgggca atgtcctggc gaccctcatc ctgat
//
LOCUS       DAXHPG010000056        10740 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXHPG010000056 DAXHPG010000000
VERSION     DAXHPG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.056"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(13..1215)
                     /gene="trpB"
                     /locus_tag="ACFM6F_001094"
     CDS             complement(13..1215)
                     /gene="trpB"
                     /locus_tag="ACFM6F_001094"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC6164516.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPTPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFYPYIEEENVRLVGVEAGGLGVDTPDHAAPI
                     TSKAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1400..2110)
                     /locus_tag="ACFM6F_001095"
     CDS             complement(1400..2110)
                     /locus_tag="ACFM6F_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6164517.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDPVL
                     PSKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFGDADYFKVPSNGEAAHS
                     EKAFDAEKIKTFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPDCR
                     HICTTGGKATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKK
                     AAAYRAFFEMAGLCEKQL"
     gene            2322..3101
                     /gene="rsmA"
                     /locus_tag="ACFM6F_001096"
     CDS             2322..3101
                     /gene="rsmA"
                     /locus_tag="ACFM6F_001096"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC6164518.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNNIEGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            3274..4002
                     /locus_tag="ACFM6F_001097"
     CDS             3274..4002
                     /locus_tag="ACFM6F_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6164519.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVLFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            4075..4434
                     /locus_tag="ACFM6F_001098"
     CDS             4075..4434
                     /locus_tag="ACFM6F_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164520.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            4439..4879
                     /locus_tag="ACFM6F_001099"
     CDS             4439..4879
                     /locus_tag="ACFM6F_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC6164521.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGWMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGTTVSQCIIPDMTDKESYRRLKVWILWRQPKKTAETDTPS"
     gene            4911..6185
                     /gene="folC"
                     /locus_tag="ACFM6F_001100"
     CDS             4911..6185
                     /gene="folC"
                     /locus_tag="ACFM6F_001100"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC6164522.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAAHGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGLEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDNPARNRNALPFPALRGTYQLSNAACTLTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFASVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            6198..7196
                     /gene="ftsN"
                     /locus_tag="ACFM6F_001101"
     CDS             6198..7196
                     /gene="ftsN"
                     /locus_tag="ACFM6F_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6164523.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            7189..7686
                     /locus_tag="ACFM6F_001102"
     CDS             7189..7686
                     /locus_tag="ACFM6F_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC6164524.1"
                     /translation="MNNLPVADLLVFAIIAACIVISTMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            7895..9439
                     /gene="purF"
                     /locus_tag="ACFM6F_001103"
     CDS             7895..9439
                     /gene="purF"
                     /locus_tag="ACFM6F_001103"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC6164525.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNTDNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMVAFRDPYGIRPLALGSQTDEAGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTIIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLKKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            9539..10030
                     /gene="greB"
                     /locus_tag="ACFM6F_001104"
     CDS             9539..10030
                     /gene="greB"
                     /locus_tag="ACFM6F_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC6164526.1"
                     /translation="MSIETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            10089..10715
                     /locus_tag="ACFM6F_001105"
     CDS             10089..10715
                     /locus_tag="ACFM6F_001105"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC6164527.1"
                     /translation="MRKIRTKICGITTPEDAAAAAAAGADAVGLVFFQGSSRVVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFHRPYIK
                     AIRVQTTSDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATTNRLSR"
BASE COUNT         2679 a         3079 c         2766 g         2216 t
ORIGIN      
        1 agacggacga ggttacagtt taatcccttt gagcttcgcg acggtattga tgtctttgtc
       61 gccacgaccg gataggttga ccagaatcac ttggtctttg cccattttcg gcgcgttttt
      121 caccgcccaa gcaacggcgt ggctggattc gagcgcgggg atgatgccct cgaagcggca
      181 gagcaagtca aaggcttcga gtgcttcgtc gtctttggca acagtgtatt cgacgcgctt
      241 gatgtcgtgc agatggctgt gttccgggcc gatgccgggg taatccaagc ctgcggaaac
      301 agagtgtgta cccaagactt gaccgttttc gtcctgcatc agatagctgc ggaaaccgtg
      361 caatacgccg ataggggctt tgctggtaat cggtgcggcg tggtcagggg tatccacacc
      421 caaaccgcct gcttctacgc cgaccaaacg tacgttttct tcttcgatat agggatagaa
      481 caaaccgatg gcgttggagc cgccgcccac gcaggcaacg gcaacgtcgg gctgtctgcc
      541 gatggcttcc tgcatctgcg ctttagcttc gttgccgatc acgcattgga aatcgcgcac
      601 catttcggga tacggcgtgg ggccggcggc ggtaccgatg atgtagaatg tgtcatccac
      661 gcgggcgacc cattcgcgca tggcttcgtt catcgcgtct ttcagcgtgc ggctgccgct
      721 ttctacaccg accacgttcg cacccaataa tttcatacgg aacacgttgg gcatttggcg
      781 ttggatgtcg tccgcgccca tatacacgtc gcaagtcata ccgaagcgtg cggcaacggt
      841 ggcactcgcc acgccgtgct gacccgcgcc ggtttcggcg atgacgcgtt ttttacccat
      901 acgttttgcc aacagtgcct gaccgatggt gttgttgact ttgtgcgcgc cggtgtggtt
      961 caagtcttcg cgcttcaacc aaatttgcgc gccacccaaa tgctcggaca atcgcgcggc
     1021 atggtaaacg gggctgggcc tgccgacata atgtttcaaa tcatggcgga acgcttccca
     1081 aaattcagga tcgtttttcg ctgccttata ggcatccgcc agctcttgca aggcgggaat
     1141 cagggtttcg gagacataaa gcccgccgtg ttcgccgaaa aagcccttct cgtcgggcgc
     1201 gtggtagttc ttcatcagat ttcgtccttg tgcttcaaat tttcagacgg caatttatag
     1261 tggattaaca aaaaccggta cggcgttgcc ccgccttgcc gtactatttg tactgtctgc
     1321 ggcttcgccg ccttgtcctg atttttgtta atccactata tttttcaaac atgccgtccg
     1381 aacgggaaat tgtcggcaat tataactgtt tttcacacaa acccgccatt tcgaaaaacg
     1441 cccgatacgc cgccgccttt ttcgccaaac tcaaaggata ggcacgcgaa gtcgaaggca
     1501 ggcgcgtcag ggtcaaatcc cgtccggcaa acggaaacgg cacggtttcg cccgttttcg
     1561 gcattttgat gccgccaccc tgaatatcga gcaggatttc cgtcgccttg ccgcctgtcg
     1621 tacaaatatg gcggcagtct ggtattttcg ccaacaccgc cgccaaatcg acggtttcga
     1681 ctacctttaa aaacttgtcg gacgcattgc cgtgctgacg taccgccttc aacacggtcg
     1741 ggcaggacgc aatcccccgt tcgtgcaaaa acgttttgat tttctccgcg tcaaacgctt
     1801 tttcagagtg cgcagcctcc ccgttggacg gcactttgaa ataatccgca tcgccgaaaa
     1861 acaccagccc gtaaacgcgc cacatatcgt tttggaaatt cggataatga aactgcatcg
     1921 cgcgtttgtc ttccttgggc ggaaacgtcc ccatcatcat gaccgcagcc ttcgacggca
     1981 acacgggatc gaagggatgg gtttcgattt caagcagatt ttcagacacg ttcaatcctc
     2041 caacaggcaa aacggcagac agccggtttt tgcaccaaac cgccaaacac ggtaagcctt
     2101 caaacagcat tatcacgccc tgccgttttc ggttacaatg ccgacatcaa acggatactg
     2161 taaacacatt tatgtttcaa cacacaggac gacacataaa gcaccgccct atgtgccgtc
     2221 ctgatttgga aggagttacg cccctcccaa atagagtctg attctaccgc cccgaaggac
     2281 agatgtccaa gtggcggggt ttcaaccgaa aaggaaacac gatgaaagaa cacaaagccc
     2341 gcaagcgttt cgggcagaat tttttgcagg acacgcggat tatcagcgat attgtcaacg
     2401 ccgtgcgccc gcaggcggat gatgtcgtga ttgaaatcgg cccgggtttg gcggcgatta
     2461 ccgaaccttt ggcgaaaaag ctgaaccgcc tgcacgttgt cgaaatcgac cgcgacatcg
     2521 tatgccgtct gaaaacgctg ccgtttgcgg ataaactcgt cattcatgaa ggcgacgtgt
     2581 tgcagtttga tttcaacaac attgaaggca aaaagaaaat cgtcggcaac ctgccgtaca
     2641 acatttccac gccgcttttg ttcaagctgg cggaggtggc ggacgatgtc gtcgatatgc
     2701 actttatgct gcaaaaagaa gtggtcgagc gtatggttgc cgcgccgaaa agcaacgact
     2761 acgggcgttt gggcgtgatg ctgcaatatt ttttcgatat ggaaatgctg attgacgtac
     2821 cgcccgaatc gttcgaccct gcgccgaaag tggattccgc cgtcgtccgt atgattccgg
     2881 tgaaacaccg catcggcaag gcggacgatt tcgagcattt cgccaaactg gtgaaactcg
     2941 ccttccacca gcggcgcaaa accatacgca acaatctgaa agagcttgca ggcgacgatg
     3001 atttgcaggc agtcggcatc aatccgcaag accgtgccga acatatcgcg ccggagaaat
     3061 atgtggcgtt gagcaattat ctagcgggca aggccgtctg aaaagggagt aagccgatga
     3121 cgaaatacct gcttttaccc ctgatactgc cgcttgcagc ctgcctgacc aatccgaacg
     3181 accgtttttt tgacggcaga cgctacacgc ttcatcatat ggagctcccg taacgacaac
     3241 ctgcaacggc aaatgccgtc tgaaagaaaa gacatgatca aattcaaaaa cgtacacaaa
     3301 catttcaaag acctgcacgt catcaacggc gtgaacttgg aaatcaaaaa aggcgaagtg
     3361 gtcgtcgtct gcggaccttc gggcagcggc aaatccacac tcatccgcac cgtcaaccag
     3421 cttgaaagca tcgaaagcgg cgagatttgg gtggacggtg tcaacgttgc cgacccaaaa
     3481 accgatttga acaaaatccg cgaagaagtc ggctttgtgt ttcaaagttt caacctctac
     3541 ccgcacctga ccgtgttgga aaacattacc ctcgcaccga tgaaggttaa aggtcaaaat
     3601 gcggaacagg cagagaaaaa agcgatggaa cttttggaac gcgtcggact ggcacacaaa
     3661 aaagacgcct tcccttccca actttccggc ggccagcagc aacgcgtggc gattgcacgc
     3721 ggtttggcga tggaaccgcg cgtaatgttg tttgacgaac cgacctccgc actcgacccc
     3781 gaaatggtcg gcgaagtgtt gaaagtgatg aaagacttgg cggaaagcgg tatgacgatg
     3841 atgtgcgtaa cccacgaaat gggttttgcc cgcgaagttg ccgaccgagt gctttttgtc
     3901 gataaagggc aaatcctcga agacgaaacg cccgaagcgt tttttaccaa cccgaaacac
     3961 gaacgcgcca aacaattcct gcaacaggtc atgacccact gatgctatgc cgtctgaaaa
     4021 ccgaacgttc agacggcata gcccgacacc gcaagatgat tgagaaagaa aaccatgtat
     4081 tttgttgacc gcaccgccgt cgtcctcaag ccgaccgtcc gctttttgga atggctcaaa
     4141 agcaccgacg aaaatatgcc cgacctgacc atagaacagc ttcgtgccaa ctgttccgtc
     4201 ttcctcgttc cgcagttcga cgaaccggaa gccgtcgttt cctatttcga cgaacgctac
     4261 cgccagattt tcgaggcgga gctggcaggt tgggacatcg ataaggataa atggccgcaa
     4321 gatatggggc tgaaggcgtt ttgggaattt ttcgacatcg aaatccacga tatggtgttg
     4381 gacatggaag aagcggaatt gaacattacc cccgtgttcg acaacatgat gtaaggctgt
     4441 gcgcgccttt cagacggcat tgaggccgtc ccgcatcctg aacatcctga ctgtatcgct
     4501 tcacgccgcc tcccttgccg tctgcctgac ttggttttac ggctggatga tgtggttcgg
     4561 tttggcggct ttggtggcaa gttacgcata ctcgctcagg ataacgaatc tgaaacaccg
     4621 ccatgccata accgccatta ccatagaccg cgacggacag gcggaaatcg tatccggcaa
     4681 agacaaaaca gcggcaacac ttgcgggcag cagtatggtt acgccttatg ccttattctt
     4741 acaatgggac acaggcggca caaccgtcag ccaatgcatc atacccgaca tgacggacaa
     4801 agaatcctac cggcggctga aagtctggat actttggcgg cagccgaaaa aaaccgccga
     4861 aaccgataca ccaagctgat ttcagacggc atttgaacac ggaacactca atgaaaacac
     4921 tgcaagactg gctctcgcat ttggaaaccg cccacagcgg cggcttgatc gatatgggct
     4981 tggaacgcgt aggcgaagtg aaaaaacgga tgaagctcac gccgcaatgc cccgtcgtcg
     5041 ttgtcgcggg aacaaacggc aaaggttcgg tctgcgccta cctgacacag atttacaaac
     5101 aggcgggcta caaaaccggc acactgacca gcccgcattt attgcgttac aacgaacgca
     5161 tcgccatcaa cgccgaaccc gtttcggacg atacgattat tgcctctttc gagcgcatcg
     5221 aagccgcgca cggcgaaata tcactgactt attttgaatt caatgccttg gcggctgtcg
     5281 acatcttcat gcgcgaacag gttgacgtga tgatattgga agtcggcttg ggcggacgct
     5341 tggacgcggt caacgcattt gacggcgatt gcgcggtggt taccagcgtg gatttggacc
     5401 atcaggcgtt tttgggcgat acggtcgaac aggtcggcct tgaaaaggca ggcgtgttcc
     5461 gcagcggcaa acctgccatc tgcggtcaaa accccgcgcc cgcatcattg gtcgcacacg
     5521 ccgaagccat aggtgcaaaa ctgctgatgg tgcagcgcga tttcgagttt cacgcgatgg
     5581 aaaacatcca atggaactac cgcttccggc cgcaacattc agacaacccc gcgcgcaacc
     5641 gcaatgccct gcccttcccc gcattgcgcg gcacatacca actttccaac gccgcctgca
     5701 cgttgaccgt attggaatgc ttgaacgaca aattgcctgt ggacatcggt gcaatcaaac
     5761 gcggattgct gctggttgaa aatcccggac gcttccaagt cctgcccggc cgtccgctga
     5821 ccgttttgga tgtcggacac aatccccacg ctgcccgcgc cctgcgccgc agcctgatta
     5881 atttggcgtt tgcgcaaaaa cgcaccgccg tattcagtat gttgtccgac aaagacatag
     5941 acggcgtgtt ggaaatcgtt aaagaccagt ttgacgagtg gtatatcgca ccgttggacg
     6001 taccgcgcgg catgacgctc gagagcctgc aaaacaaact gcacgaacac agcatcgaaa
     6061 acatacagac attcgcctcc gtccgcgaag cataccgggc ggcagcctca aaagcgggcg
     6121 agaacgacag aatcgtcgtt ttcggctcat ttcataccgt tgccgatgtc atgtcggtac
     6181 tgtaaaagga aacttctatg tccgaaaaca aacaaaacga agtcctgagc ggttacgaac
     6241 aactcaaacg gcgcaaccgc cgccgcctcg taacggcaag ttgcctggtt gccgcctcct
     6301 gcatcctgct ggcagccgcc ctcagttccg gccctgccga acagactgcc ggcgaaacaa
     6361 gcggcgtaga aaacaaagcg gcaggtgcgg cacaaacccc tgccttgaaa tccgccgccg
     6421 acaaaccgca ggacttggca ggcgaagaca agccttctgc cgccgacagc gaaatcagcg
     6481 agcctgaaaa cgtaggcgcg ccgctggtgc tgattaacga ccgcctcgaa gacagcaaca
     6541 tcaaaggttt ggaagcatcc gagaaactgc aacaggcaga aaccgccaaa accgcaccga
     6601 agcaggcaaa acaacgcgct gccgaaaaag tgccggcaac tgccgacagt acggatacgg
     6661 tagcggttga aaaaccgaaa cgcactgccg aaacaaaacc gcaaaaagcg gaacgcactg
     6721 ccaaagccaa gcccaaagcc aaagaaacca aaaccgccga aaaagttgcc gacaaaccga
     6781 aaactgccgc cgaaaaaacc aaaccggata cggcaaaatc cgacagtgcg gtaaaagaag
     6841 cgaaaaaagc cgacaaggct gaaagcaaaa aaacagccga aaaagaccgt tcggacggca
     6901 aaaaacacga aacggcacaa aaaaccgaca aagcggacaa gaccaaaacc gccgagaagg
     6961 aaaaatccgg taaaaaagcc gccattcagg caggttatgc cgaaaaagaa cgcgccttaa
     7021 gcctccagcg caaaatgaag gcggcgggta tcgattcgac catcaccgaa attatgaccg
     7081 acaacggcaa agtttaccgc gtcaaatcaa gcaactataa aaacgcaagg gatgccgaac
     7141 gcgatttgaa caaactgcgc gtgcacggca tcgccggtca ggtaacgaat gaataacctc
     7201 cccgttgccg acctcctcgt cttcgccatc atcgccgcct gcatcgtcat ttccacgatg
     7261 cgcggcgtga ttgcggaggc aggttcgatg gcggcatggg tggtttcctt cttttttgcc
     7321 aaactctttg ccgcaccctt cgccgacctc gcctttgcct cgttccaacc ccgcctgttt
     7381 gcattggctc tgtcgttcat ttccctgttc gtcattgcct gtctgatcca gaaaatgctc
     7441 cgttcgctgc tgaccggcgc agtttcggcg gtcggtttgg gctttgccaa ccgcattttg
     7501 ggcggcgtat tcggtgcatt gaaaggcgtt ttgattgtta ccctgctggt catgcttgct
     7561 tcaaaaaccg acctgcccga taccgaagaa tggcggcaat cttacacact gccgtttttc
     7621 gtatcgcttt ccgaagccgt gttgaaccat agcggcggca cggcggaaac tccggaagac
     7681 gattgattgc cctgctggcg tttcagacgg cattctcttt tatgccgtct gaaatgtttt
     7741 tttcctttcc tcagccatat cacagattaa atcttatcct ccataaaccc acctatctgt
     7801 ccttcgctca aatattgtca acaattcagg caaaaaaccg ccgtttgctt tataattccg
     7861 ttttaccctt aaacctcgaa tcggagaaga cgatatgtgc ggtgtattag gtttggtcag
     7921 tcatgagccg gtaaaccagc ttctgtacga cggcttgcag atgttgcagc acagggggca
     7981 ggatgcggcg ggcattgcaa cggccgaagg cggcaccttc catatgcaca aaggcaaagg
     8041 catggtgcgc gaagtgttcc gcacacgcaa tatgcgcgat ttggtcggca acgccggtat
     8101 cgcccacgtt cgttatccta ctgcgggcaa cgcgggcagc agcgcggagg cgcagccttt
     8161 ctacgtcagc tcgcctttcg gcattgtttt ggcgcataac ggcaacctca ccaacactgc
     8221 cgaactttat gaaaacgtgt gtaacaaaca cctgcgccac gtcaacacca gctccgattc
     8281 cgaagtcttg ctcaacgtat tcgcgcacga attacgccgc gaagtctcta aaaacgccga
     8341 cccacaccgg ctcaataccg acaatatttt caacgccgtt gccgaagtcc accgtctggt
     8401 gcgcggcgca tacggcgtgg ttgccatgat tgcgggctac ggcatggtcg ccttccgcga
     8461 tccttacggc atccgtccgc tggcattggg ttcgcaaacc gacgaagcag gcagaaaatc
     8521 ctatgccgtc gcctccgaat ccgttgcctt caacgcgctt gcctacgatt tggaacgcga
     8581 catccggccg ggcgaagcag tatttgtcgg ctttgacggc acaatcatcg cccgccaatg
     8641 cagcgaccgt gccaaactca gcccctgcct ttttgaatat gtttattttg cccgccccga
     8701 ctctgtgatt gacggtgtat cggtttacca atcgcgcttg gatatgggcg tgtccttggc
     8761 ggaaaaaatc aaacgcgagc tgcccgtgga cggcatcgat gtcgtgatgc ccatccccga
     8821 caccagccgc cccagcgcga tggagcttgc cgtccacctc aaaaagcctt accgcgaagg
     8881 tttgattaaa aaccgctata tcggccgcac ctttattatg cccggtcagg cgacgcgcaa
     8941 aaaatccgtg cgccaaaaac tcagcccgat ggaaaccgaa tttgcaggca aaagcgtgtt
     9001 gctggtggac gactccatcg tgcgcgggac gaccagccgc gaaatcgtcg aaatggtacg
     9061 cgcggcgggc gcgcgcaaag tctatatcgc ctccgccgcc cccgaagtgc gctatcccaa
     9121 cgtatacggc atcgatatgc ccacgcgcga agagttgatt gccaacgggc gcagcgcggc
     9181 ggaaatcgcc gccgaaatcg gcgcggacgg catcgtattt caagacttgg gcgatttgga
     9241 agccgtcgtc aaagcactca atccgaaaat cgaatccttc gattcgtcct gtttcaacgg
     9301 catttatcag accggcgaca tcgatgatgc ctacctcgac cgcctgtctg ccgagaaatc
     9361 cggctgcggc ggcctgaaaa tccacccgag caggatggaa cacagcatca gcatcagcga
     9421 tgcgggcgac gaagaataaa gcttcccaaa ccgcgatgcc gtctgaaatc cgaacgccgg
     9481 gttttcagac ggcatttccc atatcggcat cacatttccc atttcatccg cacgcaatat
     9541 gagtatcgaa accaaaaact acattacgcc cgccggatgg caggcattga aagacgagct
     9601 ttatcaactg gtcaataaag aacgccccga aatcgtccaa atcgtcaact gggcggcagg
     9661 caacggcgac cgcagcgaaa acggcgacta tctttacggc aaacgccgta tgcgcgaaat
     9721 tgaccgccgc atccgtttcc tgaccaagcg tttggaagct gccgtcgtcg ttgatcccga
     9781 attgcgcgaa gcgaccgacc aagtgttttt cggcgcgacg gtcgggctgt tgcgcggcga
     9841 cgggcgcgag caaaccgtca aaatcgtcgg catcgatgaa atcgataccg cgcaaaacaa
     9901 aatttcttgg atttcgccgc tggctcgttg cctgattaaa gcacgcgaag gcgacgaagt
     9961 ggttttgaac acgccggaag ggcgtgagga aattgaaatc ctttctgtcg aatacatcaa
    10021 aatcgactga tcgggaatgc cgtctgaaac ggcacaaacg cgccgtttcc caaataaagg
    10081 aataccgaat gaggaaaatc cgcaccaaaa tctgcggcat caccacaccg gaagacgcag
    10141 cggccgccgc agcggcaggt gcggatgccg tcgggctggt ctttttccaa ggcagcagcc
    10201 gggtcgtcga tattgcccgc gcaaaaaaaa tcaccgccgc actgccgccg tttgtcagcg
    10261 ttgtcgccct tttcgtcaac gaaagcgcgc aaaacatccg ccgcatcctt gccgaagtgc
    10321 cgatacacat catccaattc cacggcgacg aagacgacac attctgccgt cagttccacc
    10381 gcccctatat caaggccatt cgtgttcaga cgacatcaga catccgaaac gccgccgacc
    10441 gcttccccga cgctcaggca ctgcttttcg atgcctacca tccttcggaa tacggcggca
    10501 ccggacaccg cttcgactgg acgctgctgg cggaatattc gggcaaaccg tgggtacttg
    10561 ccggcgggct gacccctgaa aacgtcggcg aagccgtccg catcaccgga gcggaagcgg
    10621 tcgatgtatc cggcggcgtg gaagcgtcta aaggcaaaaa agacccagcc aaagtcgccg
    10681 cctttatcgc aaccaccaac cgcctatccc gttaaagcaa caaaaattgc cgccggaatg
//
LOCUS       DAXHPG010000057        10540 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXHPG010000057 DAXHPG010000000
VERSION     DAXHPG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.057"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            54..1064
                     /gene="waaF"
                     /locus_tag="ACFM6F_001106"
     CDS             54..1064
                     /gene="waaF"
                     /locus_tag="ACFM6F_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC6164528.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRYFAELGKHYSAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            1119..1565
                     /gene="smpB"
                     /locus_tag="ACFM6F_001107"
     CDS             1119..1565
                     /gene="smpB"
                     /locus_tag="ACFM6F_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC6164529.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            1931..3973
                     /locus_tag="ACFM6F_001108"
     CDS             1931..3973
                     /locus_tag="ACFM6F_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC6164530.1"
                     /translation="MYYETRSKQASKQKFKKSFIISLFFSILYTSPLLAVDYVYDNSK
                     LTNEQIDRLKKLRDKSSEYWKQETYLLKPTNGSSQNSLKVPALYSKTHQFKNINNSKK
                     ISFYDKEYTEDYLVGFARGFGVEKRNGEEEKTLQQYFKDCVNTGNSNNDKCEQPSFEN
                     SGPRLIKSDIFALAPQIKNSHINSEILSVGNYIEWLRPTLNKLPSWQEHLYSGLDPFH
                     YIEVTDNSHVIGQTISLDEFRLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKE
                     DYAGGARFRFAYDLKDKVSETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNG
                     TADKHAFRLSGKHQKGIYTLSLQQRPEGFFTKVQERDDISIYAQQAQAANTLFALRLN
                     DKNSDIFDRTLPRKGLWLRVIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQ
                     LSVGLMSGQAEQRSTFRNPDTDNLTTGNVKGFGAGIYATWHQLQDKQTGAYADSWVQY
                     QRFRHRINTEDATERFTSKGITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLG
                     VNGKFSDSENAHVNLLGSRQLQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGV
                     EIDGERRVINNKTAIESQLGVAVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(4154..5521)
                     /locus_tag="ACFM6F_001109"
     CDS             complement(4154..5521)
                     /locus_tag="ACFM6F_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC6164531.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(5631..5927)
                     /locus_tag="ACFM6F_001110"
     CDS             complement(5631..5927)
                     /locus_tag="ACFM6F_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164532.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPATEAPAA
                     EAPAAEAPAAEAPATEAPAAEAPAAEAAATEAPAAEAAASEAPAAEAPAAEAAK"
     gene            6400..6882
                     /locus_tag="ACFM6F_001111"
     CDS             6400..6882
                     /locus_tag="ACFM6F_001111"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6164533.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            6969..7781
                     /gene="hpnC"
                     /locus_tag="ACFM6F_001112"
     CDS             6969..7781
                     /gene="hpnC"
                     /locus_tag="ACFM6F_001112"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC6164534.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(7852..8322)
                     /locus_tag="ACFM6F_001113"
     CDS             complement(7852..8322)
                     /locus_tag="ACFM6F_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC6164535.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            8413..10254
                     /locus_tag="ACFM6F_001114"
     CDS             8413..10254
                     /locus_tag="ACFM6F_001114"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC6164536.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         2637 a         2277 c         2880 g         2746 t
ORIGIN      
        1 ttttccaacg gtttcgcggt aaaattctgc ctgtctgatt tcggtttctt gttatgtcca
       61 tcaaaatcct gattatttct cccagttgga taggcgactg cgtgatgacc cagcccttgt
      121 tccgccgttt gaaggaactt caccccggtt gcacgattga tgtgttcgca ccgaagtggt
      181 cgatggcggt gtttgagcgt atgccggaag tgaatgagat tcttgaaaat ccgttcggac
      241 acggtgcgtt ggagttgaaa cgccgctggc gggtcggtag ggatttgggg cggcgcggat
      301 acgatcaggt tatcgtgttg cccggttctt tgaaatcggc aatcatcgcg ctggcgacag
      361 gtatcggtaa aaggacgggt tatgtcggtg aaagccgtta ttttctgttg aacgatatac
      421 gcaggctgga taaggaacgt ctgcctttga tggtggacag atatacggct ctcgcgcatc
      481 cgagccagga ggattttgac gggcattcgg gattccccga gttttccatt gatgaacggc
      541 ggcgggaaat ttctgtcgaa acctttggct tggatattgg aaagcctgtt ctggcttttt
      601 gtccgggtgc ggaattcggg ccggcaaagc gttggccgac aaggtatttt gccgagttgg
      661 gcaaacatta ttcggcggcg ggttggcagg tttggctgtt cggttcgcaa aaagatgatg
      721 aaattgccga ggaaatcaac tgcctttcag acggtatgtg tgtgaattta tgcggaaaga
      781 ccgatttgtc gcaggcaatg gatttgctgt cgttggcgga cacggtcgtg tgcaacgaca
      841 gcgggctgat gcacttggcg gcggcattgg gcaggaaggt ggtggcggtt tacggttctt
      901 ccagcccgac gcatacgccg cctttgagcg acagggcgaa aatcgtcagc ctgcacttgg
      961 aatgttcgcc ctgttttaag cgggaatgtc cgctggggca taccgactgc ctcaacaggc
     1021 tgtatcccga gaagattgtg caggcggttg aagaggcggt atgatgggtt ctgttttcgg
     1081 gtttatgccg tctgacggcg gatattgata ggatgtttat ggctattgcg aacaataaaa
     1141 aagcatttca cgattttttc attgaagacc ggattgaagc cggtttggtc ttggaaggct
     1201 gggaagtcaa agcaatccgc gccgcgcgcg tacagcttaa agagagttat atctattgga
     1261 aaaaagacgc gttttatctg gtcggctgcc atattacggc tttgcccaca gcctcgacac
     1321 acattaaacc agatgccgta cgtccgcgca agctcttgtt gaaccagtcg gaaatcaaca
     1381 aattaatcgg caaaaccgaa cgtgccggct acaccatcgt gccgctggat ttgcattttt
     1441 cgcgcggaaa aatcaagatg gaaatcggtt tggcaaaagg taagaaacag cacgacaaac
     1501 gtcagagtat gaaagaggcg gattggaaac gcgagaaaca gcggttgatt aagcatacgc
     1561 gctgattgtg gaaacagtgc ttcagagggt atgggcaagg ctgccgcctg atgtgtaaac
     1621 gttctacacc taaaccagca tccgctttaa tcagcacagc atgatgcccc aaaccttcgt
     1681 caaaggtttg agcaatttga tgataaattg gcattagcca agggtaaagt gcggtcattt
     1741 ttcctgaaat ttttgtaaat aacgagggca attatacaca ttttcaaact ggcttgacaa
     1801 aatagcaaaa agcaaacgtt tacctttaat aaataagaaa taaatagaga aaaatgtgag
     1861 ctaaatgaca ttttttgttc agttttatgc taaaatccac gccccattta tttttacttg
     1921 acaaaggaaa atttattatg aaactcgaag caagcaagca agcaagcaga agtttaaaaa
     1981 atcatttatt ataagtctat ttttttctat tctttatacc tctccgcttt tggctgttga
     2041 ttacgtttac gacaattcta aattaacaaa tgagcaaatt gatcgcttaa aaaaacttag
     2101 agataaaagc agcgaatact ggaaacaaga aacttatctt ctaaaaccga cgaatggtag
     2161 ctctcagaac tctctcaaag ttcccgcact atactctaaa acacaccaat tcaaaaacat
     2221 caacaactca aaaaagattt ctttttatga caaggaatac actgaagatt accttgttgg
     2281 cttcgcccga ggtttcgggg ttgaaaaaag aaatggggaa gaagaaaaaa cactccagca
     2341 atattttaag gactgtgtaa atactgggaa ttccaataat gataagtgtg aacaaccatc
     2401 cttcgaaaac tctggcccaa gattaataaa gagcgatatt ttcgccttag ctccacaaat
     2461 aaaaaatagc catatcaaca gcgaaatttt atctgtaggt aattatatcg aatggcttcg
     2521 cccaacactt aataagcttc ctagttggca agagcatctc tattctggtt tagatccatt
     2581 ccactatatt gaggttacag acaactcaca cgttatcgga caaacaatta gtttggatga
     2641 attccgctta gaaaattctc tctgggaacc ccgttgggat tctgatgttg gggaattaaa
     2701 aacaacgaat gcagacatcc gttttaatac taaaagtgaa agtttactcg taaaagaaga
     2761 ttatgctggc ggagctcgtt ttcgttttgc ttacgatcta aaagataaag tctctgaaac
     2821 accagtttta acttttgaaa aaaatataac tggcacttcc gacatcatct tcgaaaatcc
     2881 aatagacgat ttaaaaagtc tcgacgggca tcaaattatc aaagtaaatg gcacagcaga
     2941 taaacacgca ttccgtcttt ctggcaaaca ccaaaaggga atttatacgc tttctttaca
     3001 acaacgccca gaaggctttt ttaccaaagt gcaagaacgc gatgatattt cgatttatgc
     3061 acaacaggct caagccgcca ataccttatt cgccttgcgt ttgaacgaca aaaacagcga
     3121 tatttttgac cgcactttac cacgcaaagg cttgtggttg cgtgtgattg acggacattc
     3181 caaccaatgg gtgcaaggca aaacagcacc tgtagaaggc tatcgtaaag gtgtgcaact
     3241 cggtggcgaa gtattcacat ggcaaaacga aagtaatcaa ctttccgttg gcttaatgag
     3301 cggacaagca gaacaacgca gcactttccg caatccagat acagacaatc ttacaacggg
     3361 aaatgtgaaa ggctttgggg cgggcattta tgccacttgg catcagcttc aggacaaaca
     3421 gacaggtgcg tatgcggata gctgggtaca atatcaacgc ttccgccacc gcattaacac
     3481 tgaagatgct acagaacgtt ttacttcaaa aggtattacc gcctcaattg aagcgggtta
     3541 caatgcttta ttggcagaac acttcactaa aaagggcaac agccttcgtg tttacctaca
     3601 accacaggca caattgactt atttgggggt aaacggaaaa ttcagcgata gcgaaaatgc
     3661 ccacgtgaat ttacttggct cccgccaatt acaaactcgg gttggcgttc aagctaaagc
     3721 gcaattttcg ctctataaaa atatcgctat tgaacctttc gccgctgtga acgcgcttta
     3781 ccacaacaaa cctttcggtg tggaaataga cggagagcgt cgagtgataa acaataagac
     3841 ggcaattgag agccaattag gtgtagcagt gaaaatcaaa tctcacttaa ctttacaagc
     3901 aacattcaac cgccaaacag gcaaacatca tcaggctaaa caaggtgcgt tgaatttaca
     3961 gtggacgttt taagttaagt tgtaacaaaa taaaatcggc agagaaattt actgccgatt
     4021 tttttatttt aaaaatgcgg tcatttttcg tgtcatttcc accaattttt taccgcactc
     4081 acgagctcgc gctaaaccga caatcggatc tattgtccct ttctttgttg tgtatgcaaa
     4141 tcagaaatgc cggttacgga agcagtttgc ccggattcat aatgttatat ggatcaaggt
     4201 gttgtttgat gcttttcatc agggcgattt cggcaggcgt gcgtactttg tccagccact
     4261 gttttttgat gatacctatg ccgtgttcgg cggcaatcgt gccgttgcag gcaagaacgt
     4321 tgcgataaac tgcgtcgttg atgtcgtttt cgtaacgata gacttcattg ctgaggattt
     4381 cgggcaggaa agtattgtag tgcaggctgc cgtcgccaag atgtccgaag cagacgattt
     4441 gtatgccttt aaaattctgt tccaaatctt tggcgcaccg gcggacaaag tcggcaacgc
     4501 gtccgatagg aatggcaata tcgtgtttga tgctggtgcc cagtttgcgt tgcgatgcgg
     4561 agatgttttc gcgcaatgcc cacatatgga tacgttcttg ttcgctttgt gccaatacgc
     4621 tgtcggtaaa tcctttttta taaagaaatt cgacaagccg atcatcaaga ttgctgtcgg
     4681 gtaatgagtc ggtcaactca agtaaaatat gccattctga atgtgtcggc agggggagtt
     4741 tgctgaattc ggaagacaac tcggcggcaa aacggccgat cagctcaaaa ctgcataggc
     4801 gttcggcaaa gtgagcttgg gtttcggtca gcaggcggac ggcggattcg atgtcgggta
     4861 tgccgaccca tgccgttgcc ttgtccgagg gacgggcaaa aagcttgagc gtggcggcag
     4921 tgatgatgcc cagtgtgcct tcgctgccga taaacagatg gcgcaggtcg tagccggtgg
     4981 tgtttttatg caggggatgg agatgggaaa ccagctcgcc gttggggagg acgacttcca
     5041 aaccgataac caggtcgcgc atcgtgccgt aacgcaatac gttcaaacct ccggcattgc
     5101 aggcgatgtt gccgccgatt tgacaggagc cttcgctggc gagactgagt gggaacagcc
     5161 tgtttgaggc ttcggctgcc tgttggacgg tttggagtac ggaacctgct tcgacggtta
     5221 tgcagttgtc tgacaaattg atgctgcgga tgcggttgag tttggaaagg ttcagcaata
     5281 cgccgttttc cgatactgcc gcgccgcaca aaccggtatt gccgccttgc ggcgtaaccg
     5341 gaatacggtg ttcgtaacaa aaacgcataa tggtttgcac gctttcaacg ctgcgcggct
     5401 gcaaaatgat gtctggtgca gacgtaaagc ggttgcgctg gtcttttaaa agcgtcggag
     5461 aaagttcggc aatttcatct gccggcagga gtcgggaaaa ttcggtatga aggtcaggca
     5521 tgtttgtcgg aagggtaggg agaagattga ggaaaggaat ctgatgccgt ctgaaaaatc
     5581 aaaaaagcag gatactgaac gtatcctgct tttttgcaag cggaaaatgc ttattttgca
     5641 gcttcggcag caggagcttc agcggcagga gcctcagaag cggcagcttc agcggcaggt
     5701 gcttcggtag cggcagcttc agcagcggga gcttcggcgg caggtgcttc agtagcagga
     5761 gcttcggcgg caggtgcttc agcagcggga gcttcggcgg caggtgcttc agtagcggga
     5821 gcttcggcgg caggtgcttc ggtagcggga gcttcagcgg ctttttcacc gccgcaggct
     5881 gccaggacca aagacaacaa agcagcggca aacagggatt ttttcatttt agtaaccttt
     5941 agaaccaatt taaattattg aaacaaatag gtctttatcg accatactac aaaccgggtt
     6001 ggaaattcat cgctgaattt gcgtaattat gcactatgga ataagggatt gcaatgtcgt
     6061 acagatggga aaaaggtttg aatgaaaagt tttctggcgg gcggattgat aaatcctgta
     6121 aaacggtttt gctgttaaaa attcgatagg gataaatatt atagagatta atataatttc
     6181 agtatcttgt atgatttatt aaggcggatt gggaaagtcg gcagacaggt cggaatggtt
     6241 ttaataatgt tgtaaaaaag ttacatcgca ataaaataac aaaaatttac attgaaatat
     6301 gtgctgtaca gtttatgaac aggttaacgg taatcggccg gcgcggcggt tgtgttagaa
     6361 tgcgcctgta atcaatctgc tgttcctgaa aggacaaata tggctatcgt gaagaattcc
     6421 gtggtttcgc tgcattatga gatgtatgat gccaacaatc agcttttgga caaaaccgaa
     6481 gaaccgattg cgtatctgca cggcggttac gacggcattt tccctttggt ggaagaggcg
     6541 ttgcacggta aggatgccgg cgatacggtc gatgtggcgc tgtcgcccga cgatgcgttc
     6601 ggcgagcagg atccggagtt ggtccgtatt gaagatgcgg gcgtgttccc tgttgaagtc
     6661 gaagtcggca tgatgtttga agccgacgat cctgaaaccg gcgatgttgt cgtctatcgt
     6721 gtaaccgatg ttgccgacgg taaggcggtg gtggacggca accatccttt ggcaggcatg
     6781 aaaatccgct ttaaggctac ggttgaaagt gtgcgcgatg catccgatga ggaaatcgca
     6841 cacggtcatg tccacggtcc gcacggtcat caccaccatt gattgccggc agcttggagg
     6901 tatctgatgc cgtctgaaaa gggtttgttc cgtttcagac ggcattttgt ttgatggatg
     6961 gagaaggtat gtcggttggg cattatgaaa attttcccgt cggttcgctg attttgccgc
     7021 gcaggttgag gaagccggtt catgcggtgt atgcgtttgc acggacggcg gacgatatgg
     7081 cggacgaggg cagtatgccg tctgaagcca ggttggcggg gttggagggt ttgcggcgcg
     7141 agttggacgt gttggcatcg ggcggccggt cggcgcatcc tttgattgca cgattggatg
     7201 ccgaggcggt tgtgccgttc ggtttggatt tgcagccgtt ttatgatttg ctttcggcgt
     7261 tttcgcagga cgtggttaaa acgcggtacg cgcatttcgg cgagctggcc gattattgcc
     7321 gacgttctgc caatcctgtc ggacgcatta tgctggcttt gtacgggaaa acggatgcgg
     7381 tgtgcgtggc gcaaagcgac ggcatctgta cggctttgca actggtgaat ttttggcagg
     7441 atgtggctgt ggattggcaa aagggcaggg tttatattcc gcaggacgat ttgttgaaat
     7501 tcggtgtttc cgaaggacag attgcggagg gtagggcgga tgcggcgttt cagcggctga
     7561 tggcgtatga gtgccggcgc gcatttcgta tgctgaaggc gggttcgcct ttagcgcgcg
     7621 aattgaaagg gcgtatcggt ttggaactcc gtatgattgt gttgggggcg cagttgattt
     7681 tgcagaaact ggacgcgtgc cgatacgatg tgtttgcaca acgccccgtt ttggataaga
     7741 aggattggtt gattatgctg aaacgcgcgt tgtggaaatg atgggggaaa tgcatgccgt
     7801 ctgaaagggg ttgttccgct tcagacggca ttttgcaggc ggttggtatg gtcaggcgat
     7861 ttcatgcggg ttcaacctgg cttgatcttc tgcacccaag ccgatgagtt tgcgcagtcg
     7921 ggtcatgtag aaattgacat ccaaaccggt gccgtagcgt tgcgccgtcc acagtgtttc
     7981 cgccagtgcc tccatcattt cgtgttcggc ttccagccag ccgcgtttgg cgcacagggt
     8041 gtcgtggatt gcgcgtatgc cgtgcggctg gtctatgccc gcctgttctt ggacggacag
     8101 atgcagcgac atatgcagga aggggttgct ttcgccgttt tcgggcagcc agtcggtgtc
     8161 cagatggtct tcgatgcgtt cgagataacg gtggtattcg ggatgggctt cgataatgcg
     8221 gagggctttc tgttccagtg cgctcagttg cagcggattg agccgctgct gccacacgcg
     8281 ggcgaaaaag cggcggacat cgtgggtatt gacatcgtac atggcgggtt cggtaagatg
     8341 tttatggaaa taggttcgga taatggcggg ttttacataa attaaatccc accgtcaacc
     8401 ttaccattta taatgcagac aaattatttg tctgaaacgg attcgattat gaaaaaactg
     8461 atttgcctgt tcgccgtatt tttgatgttg tgcggacgag ctttcgcgct ggatgcgaac
     8521 gatctgctgc cgccggaaaa ggcattcgtg ccggagcttg ccgttgccga cgacggtgtg
     8581 aacgtccgtt tcaggattgc cgacggatac tatatgtatc aggcgaaaat cgtcggcaag
     8641 accgatccgg cggatttgtt gggacagcct tctttcagca agggcgaaga gaaggaagac
     8701 gagtttttcg gcaggcagac ggtttaccat cacgaagcgc aggttgcctt tccttatgca
     8761 aaggctgtcg gcgaaccgta taaattggtt ttgacctatc agggctgtgc cgaagccggc
     8821 gtgtgctatc cgcccgtgga taccgagttt gatatttccg gcaacggcac ttaccatccg
     8881 caaaccgacg aaccggcatc cgccaaagac cgctttttgc agccttcctc tcaaaacggc
     8941 agcggggcgc tgccgccccc gaagggggat gagggcggcg acagccgttt caagctgtct
     9001 tgggatacgc tcaacgccaa tcttttggcg ttttttctcg ctggtttggg cctgagtttt
     9061 accgcctgta tgtatcccct gttgccgatt gtttccagta ttgtggtcgg cgacaaaaag
     9121 gcgggcaagg cgcgggcgtt tgtgctgtcc gtcgtttatg ttcagggttt ggctctgact
     9181 tatacgctgg tcggcattgt tgccggactg acgggcgcac tgctgaccgt atggttgcag
     9241 caggcttggg tggtattggc ggcatcggct ttaatggtcg tcttggcact gtctatgttc
     9301 gggctgttca acatccagct tcccaacgcc gtgcagtcgt attttcagaa tcaaagcagc
     9361 aggctttcag gcggtaaaat cgtttccgtc tttattatgg gcatattgtc cgcgctgatt
     9421 gtcgggccgt gcgtcgcccc gccgctggca tttgctttgg gctacatcgg tcagacgggc
     9481 gatgcggttt taggcggttt ggcactttac actttggcgt tgggcaccgg cgttccgctg
     9541 attgccatcg gcacgttcgg cgggcatatc ctgcctaggg caggcgattg gatgaatgcc
     9601 gtcaaatacg ctttcggctt tatcctgctt gccgtcgccg tttacctcgc cacgccgcac
     9661 ttgccctatt atctcgtcgt cgcgctgtac acgctgctga tgctggttcc tgcctgtatg
     9721 ctgctggtca acggacgcag gcagaaacgc cgtccgaaag ctgtggcatt cgcattgggc
     9781 ggcatattgc tgataggcgg cgcgtggttc ggctggcagg gcgcaaacgg caaaacgacc
     9841 gcgctgcacc atttcctgac cctcaatccg ccggccgaag caggcaaatc ttcggaacac
     9901 ggaaaaatgt ttgccgatac tgccgcgctg aaggcagcga tggatacggc gttgaaagaa
     9961 catcccgaca aacccgtcgt tttggatttt tatgccgact ggtgcatttc ctgcaaagaa
    10021 atggcggctt acacgctcaa tcagccggaa gtgcatcagg cagtcgatat ggaacgcttt
    10081 ttccaaatcg acgtaaccgc caacacgccc gaacatcagg cgttgttgaa agaatacggt
    10141 ctgttcggac cgccgggcgt gtttgtcgtc cgctccgacg gcagccgcag cgagccgctg
    10201 ctgggttttg tcaaagcaga caagtttatc gagtggtatg aacaaaaccg ctgattccga
    10261 tgtttcaaat gccgtctgaa gttctcagac ggcatttttg tcagaaagga aaacggcaat
    10321 atccactata cacacggaag cagtccaaag atgtgaaaac cgtgtgtata agttagtttg
    10381 cgtattgaaa ttaaaagata aaaatacgat gattaaaaaa taggcataag aaaagataaa
    10441 cacagccggt gtttgccggt gtgtgtatct tttgtggaaa aacatcttaa tgtcatgtat
    10501 tttatggaaa ataataacat gattaaaaaa tagtcaatca
//
LOCUS       DAXHPG010000058        10502 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXHPG010000058 DAXHPG010000000
VERSION     DAXHPG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.058"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..114
                     /locus_tag="ACFM6F_001115"
     CDS             <1..114
                     /locus_tag="ACFM6F_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6164537.1"
                     /translation="NGIDCKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            complement(147..1238)
                     /gene="mltB"
                     /locus_tag="ACFM6F_001116"
     CDS             complement(147..1238)
                     /gene="mltB"
                     /locus_tag="ACFM6F_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC6164538.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRVNEAQAPRADEMKKES
                     RPAFDTAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGKAKFRGARRFYAENRAVVDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1387..2814)
                     /locus_tag="ACFM6F_001117"
     CDS             complement(1387..2814)
                     /locus_tag="ACFM6F_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6164539.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDTAAFLLNDIRKDILDCRYCG"
     gene            2999..6682
                     /gene="mfd"
                     /locus_tag="ACFM6F_001118"
     CDS             2999..6682
                     /gene="mfd"
                     /locus_tag="ACFM6F_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC6164540.1"
                     /translation="MTYPIPKPREKSRWLNLSQGSLPLALARYLPHRRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDNEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQYLYLSADVFAGRLKNYGQVLP
                     DVSGKEYTLPDLAVNRQADEPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPSQQQAAPASEGEGDAITDQTE
                     FSASATNPLPSPLPQEREQSTAAISDSLKTKSSLYPIENSLHGQIQQQPAPSPVGEGW
                     GEGKAVAAQSAIAVITESDLYQYVARSRIHNRRKKHAAVSDDLLRDLAEINIGDPVVH
                     EEHGIGRYMGLITMNLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHENIALH
                     KLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINELDYQAFADGFGYEE
                     TEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAVLAPTT
                     LLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMADGTVDIVIGTHKLVQDD
                     IKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGLRDFSL
                     ITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRERLETLL
                     PEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIINRADKF
                     GLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLAMQDLE
                     IRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKLHSP
                     ALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIESHHLR
                     LMAKELGINAIDAAGEAVTVTFGKNNNIDPTEIILLIQNDKKYRLAGADKLRFTAEME
                     NIEVRINTVKNVLKTLQNRCLPK"
     gene            6831..7214
                     /gene="panD"
                     /locus_tag="ACFM6F_001119"
     CDS             6831..7214
                     /gene="panD"
                     /locus_tag="ACFM6F_001119"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC6164541.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7236..8078
                     /gene="kdsA"
                     /locus_tag="ACFM6F_001120"
     CDS             7236..8078
                     /gene="kdsA"
                     /locus_tag="ACFM6F_001120"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC6164542.1"
                     /translation="MDIKINDFTLGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8093..8533
                     /locus_tag="ACFM6F_001121"
     CDS             8093..8533
                     /locus_tag="ACFM6F_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC6164543.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSALGGSFGMWHGISSIVFMAAALLSIYLSWRLYGKNAV"
     gene            8578..9864
                     /gene="eno"
                     /locus_tag="ACFM6F_001122"
     CDS             8578..9864
                     /gene="eno"
                     /locus_tag="ACFM6F_001122"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC6164544.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYLGKGVLKAVDHVNNQIAQALIGIDASEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDAGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGK"
     gene            9878..10156
                     /gene="ftsB"
                     /locus_tag="ACFM6F_001123"
     CDS             9878..10156
                     /gene="ftsB"
                     /locus_tag="ACFM6F_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC6164545.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10197..10487)
                     /gene="yfaE"
                     /locus_tag="ACFM6F_001124"
     CDS             complement(10197..10487)
                     /gene="yfaE"
                     /locus_tag="ACFM6F_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC6164546.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         2512 a         3050 c         2686 g         2254 t
ORIGIN      
        1 aacggaatcg attgcaaatc tttcccactg ttcttgaaag aatgtgaatt tcgatttaac
       61 ttcggcacac cgtctcaaca gcttaaaatc ctgcgggatt ggtgtgggat ttagggctaa
      121 tctagtacag ccccttttaa aaaacattac aatcccgggc cgccaagcga attggcaatg
      181 tccctgaccg ccgttacata catccggctg tggttgtact gccataccgt ataaaaattg
      241 ttcaagccca aataatattc aaacacgccc ggtgcggttt ccagtttgaa caaaaccgcc
      301 ttttcatcat cggcaagctc ttcgccgggg atgatgccgt acgccttcaa atccgccacc
      361 gtccgcgtca gggcggtttt ttcgccaatg attgcctgaa catccgcacc cggcgccaat
      421 gttgcagaca ccagcatttt accgcccgtg cgccaaccgt gctgcttcat ataattggca
      481 accgatgccg cgacatcgcc gacgttgccc catatgtccc gatgtccgtc cccgtcataa
      541 tccaccgccc atttccggta gctcgaaggc ataaattgcg gcatacccat tgcacccgca
      601 tagctgccct taaaggcgaa aacatcgccg ccctcttctt ttgccagctt taaaagctcg
      661 accaattctt tttggaaaaa cccggcgcgg cgggggtaat cgaaacctaa agtcgccaat
      721 gcgtccgcca cgcggaaact gcccgtattt ttgccgtaat tcgtttcaat cccgataatc
      781 gccacgataa gctcggcggg cacgccgtat ttttgcgcca catcatcgac aactgcgcgg
      841 ttttccgcat aaaaccggcg cgcgccgcga aatttcgcct tgcccgaatt tcccgtgcgg
      901 aacacatacc acggacgcga tgtggagggt cggtgcataa tcttgacgat gtccgccttg
      961 taagccgctt tgtcaaaaaa atcctgccat tccgcccggg aaaaatcccc tttcccgact
     1021 tcatcgtcca caaaacggcg gacatttgca ttggcggcaa acccgctgtc ggataccggt
     1081 acggctgccg tgtcaaacgc ggggcggctt tcttttttca tttcatccgc gcggggggct
     1141 tgggcttcat ttacccgggg cgggcgtgcc tccatcgccg tacaggcaga caaagccgcc
     1201 aaacaaattg ccagcggcag tatttttctc tttttcataa atatgttccg aacaaatagg
     1261 gtaagtggga aagcggcgca aggggcgcgg tgcgaaatgc ggcatccgcc gcaatcggcg
     1321 gctttgtgga ataccgcgcg ttgccttttg caccgcctga aatccgtatg ccgccgtatt
     1381 tgcccgctat ccgcaatatc ggcagtccaa tatatctttg cggatgtcgt tcagcaggaa
     1441 ggctgcggtg tcttcgtgtt ccgcctgtac gaagacaaag agtcgggcga tgattttgcc
     1501 gatgaagact ttttgcaggg cgcaggcgtc ggtcagggtg tgttcctgca ggaagctgcg
     1561 gatgtcttcg ggcaaagtgt agtcgcgggc gacggcggca aagcgggcat cggtttgcag
     1621 gcggtcgagc agggtttggt ttttgtccaa cttcatgcct gcttcttttt cttcggcggt
     1681 ggcgcggacg aactggtcgt aaatttcggc ataaagcata tcgttcgggc cttcaaaaat
     1741 cgtgaagggg cggatgtcga tagcgatatt gccggcggtg tgtccgcgtt caaaaccctt
     1801 cgcgcccaaa agtttttgca acatttgcgc ggcggcgtaa gtgtattccg tggcgagggt
     1861 tttgacgatg ttcgcctcca tcagctgatg ggcgacgggg gcaacaggtg aaacggaatg
     1921 gcagacgtag cggtaaagaa tctcggaaac ctgatggcgg cgccggattt cgcggcgttc
     1981 gtaatcgacg aatctgatgt cgttgcggac gtatcgttcc aggttttcaa ggatgtattc
     2041 cataatgccg tgcgtcatgc cgatcagttg caggcggctg cggataaaga tgttttggaa
     2101 cgcgcgcaaa ccggcagcgt cgctctggga gagtttcatc acggcggttg caggcatttc
     2161 ggcatcgatg cggttgacgg cgtaacggac ggcgcgcaag ccttcggatg cgagggtttc
     2221 gcagcggatg tatgttttgg ggacgagcag caggtcgatg actttggcga gtttgccgtt
     2281 tttgcgctct ttggcggcaa cgaggaggaa gtcgctttgc gagttgccct gccagtattt
     2341 cgcggcgttg acgtaaatgg tttgtccgtc ggtatattcg tagtaagact gcatttctcg
     2401 ggcaatcgcc gcgccggagg tttcgggttc ggtaacgccc aaaccaccgc cctcgccttt
     2461 gaaaaccatg tccaaaccct gtgcgatttg cgcttcatcg ccgaactctt gcagtggctg
     2521 caacaccagc gcgccttcga tgccggtacg cagcgtaacg ggcacgccgt aatgccccgc
     2581 aatccgcaag acttcctgaa tttcaaactg gctgcccttg cgcccgccgt gttttttgtc
     2641 gaggaagggc aacagcaaac ccgcctgctt caaggcaagc catttgtctt cgggcaggta
     2701 tcgcatcagg tcgataccgt ctgaaaaaat gcggcggaat gcggattcga tgtgctttaa
     2761 aaaagcagcc gtgtccatag ttgacggctg cgcactcggt tcggtgtgta tcatcggctt
     2821 cctctgtcgg ttcccattaa tcggcggccg gtcaaaccgc ctgccacggt ttagagttaa
     2881 ttttctaaac tttaccacaa agtgcgccgg gcaacaatcc gccgaccttt cagacggcat
     2941 cgcgccccct cccgtgctaa aatgaccgtt tgcatcactg tccgccgatt gccgcactat
     3001 gacctacccc atccccaaac cccgtgaaaa atcccgttgg ctcaatctgt ctcaaggctc
     3061 gctacccttg gctttggcgc gttatctgcc gcataggcgg ctcaaagtcg tgctgaccca
     3121 agatgcggaa caggcgctac gccttcagac ggcgtggcgg tttttccgtc cgcacgatac
     3181 ggcggtgttc ctgccggact gggaaacgct gccttacgag cgtttttcgc cgcatcagga
     3241 tttggtgtcg gagcgtttgt cggcgttgtg gcagattaaa agcggcgcgg cggacgtgtt
     3301 gttcgtgccg gttgccacgg cgatgcagaa gctgccgccc gtgccgtttc tggcggggcg
     3361 cacgttttgg ctgaaaacgg gacagacttt ggatataggc cgtctgaaaa gcgatttggt
     3421 ggatgcaggt tataaccatg tttcccacgt tgtcgcggca ggcgagtttg ccgtgcgcgg
     3481 cggcattgtg gatttgttcc cgatgggcag cgaaatgccg taccgcatcg atttgttcga
     3541 cgatgaaatc gacagcatca aaacctttga caccgagacg cagcgcacca tctcccccgt
     3601 ttccgaaatc cgtctgctgc cggcgcacga gttccccacc gacaacgagg cgcaaaaaat
     3661 cttccgcagc cgttttcgcg aggaagtcga tggcaatccg aacgatgccg ctgtgtacaa
     3721 agccgtcagc aacggtcatt tcggcgcggg cgtggaatac tacctgccgc tgttttttga
     3781 aaacgagttg gaaacgctgt ttgactatat cggcgaagat gcgctgtttg tctctttagg
     3841 cgatgttcat gccgaggcaa accgtttttg gagcgatgtc aaatcgcgtt acgcgatggc
     3901 gcagggcgac gaaacctatc cgcctttgct tccacagtat ttgtatctct ctgccgatgt
     3961 gttcgcaggc cgtctgaaaa actacggaca ggtgctgccc gatgtttccg gcaaggaata
     4021 caccctgccc gaccttgccg tcaaccgcca agcagatgag ccgttgcagg cattgaagga
     4081 ttttcagacg gcgtttgacg gacggatttt gctgtgcgcc gaaagtttgg gacggcgcga
     4141 aactatgctc ggtttcttgc agcaaaacgg tttgaaagcc aaacccgtgt ccgactggca
     4201 gggcttttta tcggcacacg agccgctgat gattactgtt gcgccgttgg catacgggtt
     4261 caaactgggc gggctgcaat ctccgagcca acagcaagct gctcccgcct ccgaaggaga
     4321 aggcgatgcg attaccgacc aaaccgaatt ttccgcatcc gcaacaaacc ctctccctag
     4381 ccctctccca caggagaggg aacaaagtac cgccgccatt tcagacagtc tgaaaaccaa
     4441 aagcagcctg taccccatcg aaaatagtct gcacgggcaa atccaacagc aacctgcccc
     4501 ctcccccgtg ggggagggtt ggggagaggg caaagcagtt gccgctcaaa gtgccatcgc
     4561 cgtcatcacc gaatccgatc tctaccaata cgtcgcccgt tcgcgcatcc acaaccgccg
     4621 caagaaacac gccgccgttt cagacgacct gttgcgcgac cttgccgaaa tcaacatcgg
     4681 cgaccccgtc gtgcacgagg aacacggcat cggccgatat atgggcttga taacaatgaa
     4741 cttgggcggc gaaaccaacg aaatgatgtt gctcgaatat gcgggcgaag cgcagcttta
     4801 tgtgcctgtt tcgcaactgc atttaatcag ccgctactcc ggtcaggcgc atgaaaacat
     4861 tgccctgcac aagctcggca gtggcgcgtg gaacaaggcg aagcgcaaag ccgccgaaaa
     4921 ggcgcgcgat accgccgccg agttgcttaa cctttacgcc cgacgtgccg cccaatcggg
     4981 acacaagttt gaaatcaacg agttggatta tcaggcgttt gccgacggct tcggctacga
     5041 agaaaccgaa gaccaggccg ccgccatcgc cgccgtgatt aaagatttga cgcaggcgaa
     5101 gccgatggac cgcctcgtgt gcggcgatgt cggtttcggc aaaaccgaag tcgccctgcg
     5161 cgccgcattt gtggcggtga tgggtggcaa acaggtcgcc gtactcgccc cgaccacgct
     5221 tctggtcgag cagcacgcgc aaaactttgc cgaccgtttc gccgatttcc ctgtgaaagt
     5281 cgccagcctt tcgcgtttca acaacagcaa agccactaaa gccgcgctgg aaggcatggc
     5341 ggacggcacg gtcgacatcg ttatcggcac gcacaaactg gtgcaggacg acatcaaatt
     5401 caaaaactta ggtttagtga ttatcgacga agaacaccgc ttcggtgtcc gccaaaaaga
     5461 gcagctcaaa cgcctgcgcg ccaatgtgga catcctcacc atgaccgcca cgccgattcc
     5521 gcgtacttta agtatggcgt tggaaggact gcgcgacttc tcgctgatta ccaccgcgcc
     5581 cagccgccgc ctcgccgtca aaacctttgt caaacccttt agcgaaggca gcgtgcgcga
     5641 agccgtgttg cgcgaactca aacgcggagg acaggtattt ttcctgcaca atgaagtaga
     5701 tacgattgaa aatatgcgcg agcggctgga aaccctgctg cccgaagccc gcatcggcgt
     5761 ggcgcacgga caactgcgcg agcgcgagct ggaacaagtc atgcgcgact ttttgcagca
     5821 acgatttaac gtgttgctct gctccaccat catcgaaacc ggcatcgaca tccccaacgc
     5881 caacaccatc atcatcaacc gcgccgacaa attcgggctg gcgcaactgc accagcttcg
     5941 cgggcgcgtc ggccgcagcc accatcaagc ctacgcctac ctgctcacgc ccgaatacat
     6001 caccaaagac gcagaaaaac gcctcgacgc catcgcggcg gcagacgaac tcggcgcagg
     6061 tttcaccctc gccatgcagg atttggaaat ccgcggcgca ggcgaaatcc tcggcgaagg
     6121 gcagtccggt gaaatgatgc aggtcggctt cacgctctac accgaaatgc tcaaacaggc
     6181 cgtgcgcgac ctcaaaaaag gccgccaacc cgacctcgat gcaccgttgg gcattaccac
     6241 cgaaatcaaa ctgcacagcc ccgccctgct gcccgaaagc tactgccccg acatccacga
     6301 acggctcgtc ctctacaaac gcctcgccgt ctgcgaaacc gtacagcaaa tcaatgccat
     6361 acacgaagaa ctcgtcgacc gcttcggact gcccgagcaa cccgtcaaaa cccttatcga
     6421 aagccaccac ttacggctta tggcaaaaga attgggtatc aatgccattg atgcggccgg
     6481 cgaagcggta acggtaacct ttggcaaaaa caacaatatt gatccaaccg aaatcatcct
     6541 gctgattcag aacgacaaaa aataccgcct tgccggcgcc gataagctgc ggtttaccgc
     6601 agagatggaa aatatcgagg tcagaatcaa caccgtaaaa aacgttttaa aaaccttgca
     6661 aaacagatgc ctgcccaaat aaagccgaca ccgcaatgct gtctgaaaca ccgttttcct
     6721 tgtccgaaag ccgccattat gaatttgaag gaaactccac tataatacgg cattcagatt
     6781 ttcagacggc atcgcgcccg tcaaaccgca cacaaaccaa aaggaaatac atgttccgta
     6841 ctatgcttgg cggaaaaatc caccgcgcca ccgttaccga agccgattta aactatgtcg
     6901 gcagcattac cgtcgatcaa gacctgttag acgcggcagg catctacccc aacgaaaaag
     6961 tcgccatcgt caacaacaac aacggcgaac gttttgaaac ctataccatt gcagggaaac
     7021 gcggcagcgg cgtgatttgt ctgaacggtg ctgcggcaag gctggtgcag aaaggcgata
     7081 tcgtcatcat catgtcttac gtccaactct ccgaacccga aatcgccgcg catgaaccca
     7141 aagtcgtctt ggtagacgga aacaacaaaa tccgcgacat catctcctat gagccgccgc
     7201 acaccgtgct gtaattccgc aaacggacat cgattatgga tattaaaatc aacgacttca
     7261 ccctcggcaa caactcgccc ttcgtcctat tcggcggcat caacgttttg gaaagcttgg
     7321 attccaccct ccaaacctgt gcgcattacg tcgaagttac ccgaaaactc ggtattccct
     7381 atatctttaa agcctctttc gacaaggcaa accgttcctc catccattct tatcgcggcg
     7441 taggcttgga agaaggctta aagatttttg aaaaagtcaa agcagagttc ggcatccccg
     7501 tcattaccga cgtacacgaa ccccatcagt gccaacccgt cgccgaagtg tgcgatgtca
     7561 tccagcttcc cgcctttctt gcgcggcaga ccgatttggt ggccgcaatg gcggaaacgg
     7621 gcaatgtcat caacatcaaa aaacctcagt tcctcagccc ctctcaaatg aaaaacattg
     7681 tggaaaaatt ccacgaagcc ggcaacggga aactgatttt atgcgaacgc ggcagcagct
     7741 tcggctacga caacctcgtt gtcgatatgc tcggtttcgg cgtgatgaaa cagacttgcg
     7801 gcaacctgcc ggttattttc gacgttaccc attccctgca aacccgcgat gctggttctg
     7861 ccgcatccgg cggtcgtcgc gcacaagctt tggatttggc acttgcaggc atggcaaccc
     7921 gccttgccgg cctgttcctc gaatcgcacc ccgatccgaa actggcaaaa tgcgacggcc
     7981 ccagcgcgct gccgctgcac cttttagaag attttttaat ccgcatcaaa gcattggacg
     8041 atttaatcaa atcacaaccg attttaacaa tcgagtaaca cggtttcgcc ttatgatgca
     8101 gactttccga aaaatcagcc tgtatgccgc aaccttatgg ctcggtatgc agattatggc
     8161 gggttatatc gtcgcgccgg tgctgttcaa aatgctgccc aaaatgcagg cgggcgaaat
     8221 tgccggcgta ttgttcgaca tcctctcttg gagcgggctt gccgtttggg gcgcggtact
     8281 ggctgccgcc tttgccgccc taacccggcg gcaaaccgcc ctgctgcttt ttttattgtc
     8341 cgcccttgcc gccaaccgat tcttgattac acccgttatc gaggcgctga aatacgggca
     8401 tgagaattgg ctgttgtcgg ctttgggcgg atctttcgga atgtggcacg gcatttccag
     8461 tattgttttt atggcggccg ccctactttc gatttactta agttggcggc tctacggcaa
     8521 aaatgccgtc tgaagcctac cgttttacct cacgtcactt taatttggag aatatttatg
     8581 agcgcaatcg ttgatatttt tgcccgcgaa attttggact cacgcggcaa cccaaccgta
     8641 gaatgcgacg tactgttgga atccggcgtc atgggacgcg cagccgtacc aagcggcgca
     8701 tcaaccggtc agaaagaagc tttagaactt cgcgacggcg acaaatcccg ttacttgggc
     8761 aaaggcgtat tgaaagccgt tgaccacgtc aacaaccaaa tcgcgcaagc cctcatcggt
     8821 atcgatgcca gcgaacaatc ttatatcgac caaatcatga tcgaattgga cggtactgaa
     8881 aacaaaggta acttgggtgc caacgcgact ttggccgttt ctatggccgt agcacgcgca
     8941 gccgctgaag atgcagggct gccgctttac cgctacttgg gcggcgcagg tccgatgtcc
     9001 ctgcccgtac cgatgatgaa cgtcatcaac ggcggcgaac acgccaacaa cagcctgaac
     9061 atccaagagt ttatgattat gcccgtcggc gcaaaatctt tccgcgaagc gttgcgctgc
     9121 ggtgcggaaa ttttccacgc cttgaaaaaa ctgtgcgaca gcaaaggctt cccgaccaca
     9181 gtcggcgacg aaggcggttt cgcccccaac ctgaacagcc acaaagaagc cctgcaactg
     9241 atggttgaag ccaccgaagc cgccggctac aaagcgggcg aagacgtatt attcgcattg
     9301 gactgcgcgt ccagcgagtt ctacaaagac ggcaaatacc acttggaagc cgaaggccgc
     9361 tcctacacca acgcggaatt tgccgaatat ctggaaggcc tggtcaacga gttccccatc
     9421 atctccatcg aagacgggat ggatgaaaac gactgggaag gctggaaact gctgaccgaa
     9481 aaactgggcg gcaaagtcca actcgttggc gacgacctct tcgttaccaa cccgaaaatc
     9541 cttgccgaag gcattgaaaa aggcgtggca aacgcactat tggtcaaagt caaccaaatc
     9601 ggtactttga gtgaaaccct gaaagccgtc gacttagcca aacgcaaccg ctacgccagc
     9661 gtaatgagcc accgctccgg cgaaaccgaa gacagcacca ttgccgactt ggcagtcgcc
     9721 accaactgta tgcagatcaa aaccggttct ttgagccgtt ccgaccgcat ggcgaaatac
     9781 aaccaactgc tgcgtatcga ggaagaattg gcggaagccg ccgactaccc cagcaaagcc
     9841 gcattctacc aactgggcaa ataaaaaagg ttaaggtatg aagtgggtaa ctgtcgtttt
     9901 atccttcgca cttgtctgtt gccaatacag cctctggttc ggcaaaggca gcatcggacg
     9961 caacagcagt ctgagagaac agattgccgt tcaagaagaa aaaaaccaga cactcgccct
    10021 acgcaatcat tcccttgccg ccgaagtcta tgatttggaa aacggtcaag aagccatttc
    10081 ggaaatcgcc cgggtagaac tgggttatat ccaagacggt gaaacctttt accgactcat
    10141 caggcataac cggtaatacc gtcaaaaagc cgtccgaacc aatgttcgga cggcttttat
    10201 ttcaacaaac tgtcagacag cccctcatcc tcccccgaca aaccgcaatc cagcctgaca
    10261 tccccctcga cgcaacagca gcacggcagt atctcgtccc gccccaaaaa agccaaaggc
    10321 ggctcccgat aagtaacgct tccctccaaa atcttcactc ggcacgatcc gcaatatccg
    10381 cttcggcact gatattccac catatgcccc gtccgttcca agccttccaa cagggtctcg
    10441 ccctccaaga gctcaaaaaa acccttattc gtaccaatgc gcgccatttc cgaccaatca
    10501 aa
//
LOCUS       DAXHPG010000059        10415 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXHPG010000059 DAXHPG010000000
VERSION     DAXHPG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.059"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            55..294
                     /locus_tag="ACFM6F_001125"
     CDS             55..294
                     /locus_tag="ACFM6F_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164547.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVLSMTD"
     gene            508..1809
                     /gene="bioA"
                     /locus_tag="ACFM6F_001126"
     CDS             508..1809
                     /gene="bioA"
                     /locus_tag="ACFM6F_001126"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC6164548.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLVDGMSSWWCAIHGYNHPVLNQAVETQIKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYRQVRGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPTSRFDDEWDEADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            1806..2453
                     /gene="bioD"
                     /locus_tag="ACFM6F_001127"
     CDS             1806..2453
                     /gene="bioD"
                     /locus_tag="ACFM6F_001127"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC6164549.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QQLAAQYEIVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLIFNHIHDSSDECVAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            2468..2944
                     /locus_tag="ACFM6F_001128"
     CDS             2468..2944
                     /locus_tag="ACFM6F_001128"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC6164550.1"
                     /translation="MEHLFGKWLPDLPTPISDGISLPMVRLLHTRSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVFGEGCGRYFAARRSRFSHHGEEMLLTEYFLPELKRFIG"
     gene            2974..3864
                     /gene="ubiA"
                     /locus_tag="ACFM6F_001129"
     CDS             2974..3864
                     /gene="ubiA"
                     /locus_tag="ACFM6F_001129"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC6164551.1"
                     /translation="MNPKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            4049..4498
                     /gene="ptsN"
                     /locus_tag="ACFM6F_001130"
     CDS             4049..4498
                     /gene="ptsN"
                     /locus_tag="ACFM6F_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC6164552.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            4502..5464
                     /gene="hprK"
                     /locus_tag="ACFM6F_001131"
     CDS             4502..5464
                     /gene="hprK"
                     /locus_tag="ACFM6F_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC6164553.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            5445..6299
                     /gene="rapZ"
                     /locus_tag="ACFM6F_001132"
     CDS             5445..6299
                     /gene="rapZ"
                     /locus_tag="ACFM6F_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC6164554.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIAYLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            6381..7322
                     /gene="ylqF"
                     /locus_tag="ACFM6F_001133"
     CDS             6381..7322
                     /gene="ylqF"
                     /locus_tag="ACFM6F_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC6164555.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPADE"
     gene            complement(7413..7533)
                     /locus_tag="ACFM6F_001134"
                     /pseudo
     CDS             complement(7413..7533)
                     /locus_tag="ACFM6F_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7597..8199
                     /locus_tag="ACFM6F_001135"
     CDS             7597..8199
                     /locus_tag="ACFM6F_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC6164556.1"
                     /translation="MTEKLPPDALIEAALLTQAEPLNEKTMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            8383..10056
                     /gene="recN"
                     /locus_tag="ACFM6F_001136"
     CDS             8383..10056
                     /gene="recN"
                     /locus_tag="ACFM6F_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC6164557.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKSVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRSLGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDKIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            10166..10242
                     /locus_tag="ACFM6F_001137"
     tRNA            10166..10242
                     /locus_tag="ACFM6F_001137"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10200..10202,aa:Arg,seq:acg)
     gene            10260..10334
                     /locus_tag="ACFM6F_001138"
     tRNA            10260..10334
                     /locus_tag="ACFM6F_001138"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10293..10295,aa:Glu,seq:ttc)
BASE COUNT         2527 a         2598 c         2834 g         2456 t
ORIGIN      
        1 ataaaatagg gctgtaacct tcaatcggaa tttgttgcct gcgggatatg cggtatgaat
       61 gtttgggata tatggggcag gatggtggaa atctatcata agtataagaa gccgtgcctg
      121 gttttggcgg tggattttgt gatgggtatg gtattcatag agccgaatga ggagccgtgc
      181 atcggcagat gctatgcgcc tatgtcggag tcccctgatt ttgccaacgc tgttgcgatg
      241 gctgttgcta tgatctgtat cgtatggatt gccgttttgt ctatgactga ttgaagcgtt
      301 gggcggaggc tgcgtgaaac ggtattgggc gttgggccgt ctgattccaa tcgggcttga
      361 ggaatgcgaa acggtgtgcg cttatgttgc ggaacgattt gtttcgcggt tttgcgcccg
      421 aaacggatgg agaggtgtgg gaaacggtct gtcggagtag aatacgcgtt tgcgtttgaa
      481 tgcagcaata agaaaagaga gaaacttatg ccgtctgaac atcaacacac atcatcatta
      541 cttgatttcg accgtaccca tctgctccat ccctatactt ccatgaccga tccgcttccc
      601 gtttatcctg tcaaacgtgc agaaggggtg tttatcgaat tggcggacgg cacgcggctg
      661 gttgacggga tgtcgtcctg gtggtgtgcg atacacggtt acaatcatcc tgttttgaat
      721 caggcggttg agacgcagat taaacaaatg tcgcatgtga tgttcggcgg tttgacgcat
      781 gagcctgcgg tggagctggg taaattgttg gttgggattt tgccgcaggg gttggaccgt
      841 attttttatg cggattcagg ttcggtttcg gtggaagttg cgttgaaaat ggcggtgcaa
      901 taccggcagg tgcgggggtt gacggcgaag cagaatattg cgacggtgcg gcgcgggtat
      961 cacggcgata cttggaacgc gatgtccgtc tgcgatccgg aaacggggat gcaccatatt
     1021 ttcggcagcg cgttgccgca gcgttatttt gtcgataacc cgacaagccg tttcgacgat
     1081 gaatgggacg aggcggattt gcagcctgtc cgcgccttgt ttgaagtgca tcatgcggat
     1141 attgccgcct ttattttaga gccggtcgtg cagggcgcgg gcggcatgta tttttatcat
     1201 ccgcagtatc ttcgcggatt gcgcgatttg tgcgacgagt ttgatatcgt tttgattttt
     1261 gatgaaatcg ccaccggatt cgggcgcacg ggcaagatgt ttgcctgcga acacgcggag
     1321 gtcgtgccgg atattatgtg catcggcaag ggtttgagcg gcggctatat gacgctggcg
     1381 gcagcaatca cttcgcaaaa agttaccgaa acgatttcgc gcggcgaagc gggcgtgttt
     1441 atgcacggcc cgacgtttat ggcaaacccg ctggcgtgtg ccgttgcctg cgcttcggtc
     1501 aaactgcttt tgtctcaaga ctggcaggca aatatccgcc gcattgaaag catcttaaaa
     1561 ggccgtctga aagccgcgtg ggacattcgc ggcgtgaaag acgtacgcgt tttgggtgcc
     1621 atcggcgtga tcgagctgga taaaggcgtg gatatggcgc gttttcaagc agattgcgtg
     1681 gcacagggca tttgggtgcg cccgttcggc aggctggtat atctgatgcc gccgtatatt
     1741 atttcagacg gcgttttgac caaacttgcc gacaaaaccg tgcaaatctt gaaggaacac
     1801 agcaaatgaa aggcgtttac ttcgtcagcg gcatagacac ggacatcggc aaaactgtcg
     1861 ccaccggtat gttggcaaaa caattgttgc agcagggcaa aagcgtgatc acgcaaaagc
     1921 ccgtgcaaac cggttgccaa gacatcgccg aagacatcgc cgttcaccgc aaaatcatgg
     1981 gcatcccgat gcaggaagcg gacaaacaag ggctgaccat gcctgaaatc ttcagttatc
     2041 cagcttcgcc tcatctcgct gcccgtcttg acggcagggc tttggacttg gacaaaatcc
     2101 gcaccgccac gcagcaactg gcggcgcaat acgaaatcgt cttggtcgaa ggcgcgggcg
     2161 gattgatggt tccgctgacg gaaaacctgt taaccattga ttatatccgt cagcaaggct
     2221 atcccgtcat cctcgttacc agcggacggc ttggcagtat caaccacact ttactcagtt
     2281 tcgccgcgct caaacaatac ggcatccgcc tgcacagcct gattttcaac catattcacg
     2341 acagttccga cgaatgtgtc gcccaagaca gcctgagcta cttgaaatgc cgtctgaaag
     2401 ccgatttttc cgaagcggaa tggatggagt tggcaaaaac agacgcggta taaagattgg
     2461 gaaaaatatg gaacacctat ttgggaaatg gctgcccgac ttgcccaccc ccatttcaga
     2521 cggcatcagc ctgccgatgg tgcggctgct gcacacccgg tcgctgaccg ccgcattgcg
     2581 cgccttgccg catacatttt cggtggaact gctgaaactg ggcgaattgg agacggaatg
     2641 cggagggagg ctggtgcgcg aagttttgtt gaagctggac ggtatccctg ttgtcgcggc
     2701 aaggagcgag tgccggatag gttcggcgtt ttggcaaaac attttggact gcggcacgcg
     2761 tcctttgggc gaacgtctgt ttcaagccga tttggaaggg gcgcgttcgg cgtttgagtt
     2821 tgccgttttc ggcgaaggat gcggacggta ctttgccgct cggcgttcgc ggttttccca
     2881 tcacggcgag gaaatgctgc tgaccgagta ttttctgccc gaactgaaac gctttatcgg
     2941 ataaaatacc gttttttcaa gctgcgcggc aatatgaatc ctaaatcccc tttattttta
     3001 cgcctgtccg atcgtttgga cgtttatatc agactgatgc gggcggacaa gcccattggg
     3061 acgctgcttt tgctgtggcc gacctactgg gcgttgtggc tggcttcaga cggtattccc
     3121 gatttggcgg tattggcggc gtttacaatc ggcacgtttt taatgcgcag tgccggctgc
     3181 gtcatcaacg attttgccga ccgcgatttt gacggggctg tcgagcgcac taaaaaccgt
     3241 ccgttcgcac agggcagggt caagaaaaaa gaagcgctgc tgctgacggc atttttgtgc
     3301 ctgcttgccg cattgtgcct gattccgctg aatcatctga catggctgat gagcctgccc
     3361 gcgctgtttc ttgcgctgac ttacccgttt accaaacgct tttttccgat tccccaactc
     3421 tatctcgggc ttgccttttc cttcggtatc ccgatggcgt ttgccgccgt tgccggaaac
     3481 gtgccgcctc aagcgtggat actctttgcc gccaatgtgt tatggactct ggcgtatgac
     3541 acggtttatg caatggcgga caaagaagac gatttgaaaa tcggcatcaa aacctccgcc
     3601 gtcacgttcg ggcgttacga catcgccgct gttatgctgt gtcatggcgg ctttaccctg
     3661 ctgatggcag tattgggggc ggttatcggt gcggcatggg catattggac ggcaatcccc
     3721 atcgtcctgc tgctgcaata ccgccaatat gccgccatca aaagccgcgt ccggcaaatc
     3781 tgttttgaaa cgtttttggc aaacaacaga attggttggg tgtggtttac cgccattttt
     3841 gcccatacgt ttttcgcgaa ataaggcagg gcaatgccgt ctgaagagcc gtaaactgct
     3901 ttggacggca tttctatctg tgccgaaaag cgttaaaata tgtttttaaa acgctgtgtt
     3961 atgtcagccc gtaccgtatg cgggattgag atttgccccg gcagtcggta caatctttct
     4021 gttttgcgat gtctgaaaag agaagcttat gagccttatc ggcgaaattt tgcctttgtc
     4081 ccatattgtt ttggatatgg aggtgggcag taaaaaaagg ctgtttgagg aagcaggcct
     4141 gcttttggaa cgcgaatcct cattgtccca tgctgatgtt ttcgaatgtc tttttgcccg
     4201 tgaaaaactc ggttcgaccg gtttggggca gggcgttgcc atcccgcacg gccgtcatgc
     4261 cggcgtgaag caggcgacgg gcgcgttcat ccgcacgcgc gaacccgtcg gatttgacgc
     4321 accggacggc aaaccggttt ccctggtttt tatcttgctg gttcctgaga atgcaacggg
     4381 cgagcatttg gaggtattgt ccaaactggc cggcaagttt tcccaaaaaa gcatccgcga
     4441 atcgctgatg acggtttctt ctgcggaaga agcccgcgcc atcctgaccg aagaataaaa
     4501 tatgcccagt atctctgtcc gccgactgtt tgacgacaac caatacaagt tgcaacttgc
     4561 ctgggccgcc ggcaattcgg gtgcggacaa ccgtatcggc gtagaggcgg acaagcccgt
     4621 cctcgctttg gtcggacacc tgaatttcat ccaccccaac caaatccaag tggtcggttt
     4681 ggcggagtcg gaatatctga accgtctcga atcgggggaa acgggttatc agttcggcga
     4741 cctgttcgac atttctatgt ctttggttat tgtggcaaac ggcttgccgg tttccccggg
     4801 actgcgcgac tactgccata aaaacgatat tccactgctg acttccaaac tcgaaagccc
     4861 ctatctgatg gacgtgttgc ggatttacct gcaacgcacc ctggcggcat cgtccgtcaa
     4921 acacggcgta tttctcgatg tgtttgaaat cggcgtgctg attaccggac attcgggttt
     4981 gggtaagagc gaattggcgt tggagctgat ttcgcgcggc cacagcctga ttgccgacga
     5041 tgcggtcgag ctgttccgca tcggcccgga aacgctggaa gggcggtgtt cgcctatgct
     5101 gcgcgatttt ttggaagtgc gcggcttggg gatactcaat atccgccata ttttcggcga
     5161 aacttccatc cgccccaaaa aaatcctgca actcattatc aatttggtcg aggcagacga
     5221 cgagtatatg aagcagctcg accggttgag tatccgcacc gaaaccgaat ccatcctcaa
     5281 cgtcaacgtc cgttcggtaa cgctgcctgt cgcggtcgga cgcaaccttg ccgtattggt
     5341 tgaagcggcg gtacgcaatt acattttgca gttgcgcggt aaggacagta cgcgcgaatt
     5401 tttggaacgc catcagacgc aacttaaaga aaacgaacag aatcatgaaa atcgtcctga
     5461 ttagcgggtt gtccggttcg ggcaaatccg tcgcactgcg ccaaatggaa gattcgggtt
     5521 atttctgcgt ggacaatttg cctttggaaa tgttgcccgc gctggtgtcg tatcatatcg
     5581 agcgtgcgga cgaaaccgaa ttggcggtca gcgtcgatgt gcgttccggc attgacatcg
     5641 gacaggcgcg ggaacagatt gcctatctgc gcggactggg gcacagggtt gaagttttgt
     5701 ttgtcgaggc ggaagaaagc gtgttggtcc gccggttttc cgagaccagg cgaggacatc
     5761 ctctgagcaa tcaggatatg accctgttgg aaagcttaaa gaaagaacgg gaatggctgt
     5821 ttccgcttaa ggaaatcgcc tactgtatcg acacttccaa gatgaatgcc caacagctcc
     5881 gccatgcagt ccggcagtgg ctgaaggtcg aacgtaccgg gctgctggta attttggagt
     5941 ccttcgggtt caaatacggt gtgccgaaca acgcggattt tatgttcgat atgcgcagtc
     6001 tgcccaaccc gtattacgat cccgagttga ggccttacac cggtatggac aagcccgttt
     6061 gggattattt ggacggacag ccgcttgtgc aggaaatggt tgacgacatc gaaaggtttg
     6121 ttacgcattg gttaccgcgt ttggaggatg aaagcaggag ctacgttacc gtcgccatcg
     6181 gttgcacggg aggacagcac cgttcggtct atattgtcga aaaactcgcc cgaaggttga
     6241 aagggcgtta tgaattgctg atacggcaca gacaggcgca aaacctgccg agccgttaat
     6301 cccattaaac aattatgccg tctgaaaccc cgcttttctg tttcagacgg catttcaaat
     6361 attgagaaga aaggaaagat atggcaatcc aatggtttcc cggtcatatg aacaaggcga
     6421 aaaaagccat cgccgagcgt gcaaaaagcg ttgatatggt gattgaaatg ctggacgcgc
     6481 gtatgcccgc ctccagcgaa aaccccctgc ttgcccagct ttccaaaggc aaacccaaac
     6541 ttaaaatcct aaacaaacaa gatcttgccg accccgaacg cacaaaggtc tggcttgacc
     6601 actacaacag ccgccccgac acgcgcgcca ttgccctcga ttcctccgaa accggcgcac
     6661 acggcaaaat cacccaagcc tgccgcgcga tgattcccca ccggcaaggc atagacaaac
     6721 ccttgcgcgt cttgatttgc ggcattccca acgtcggcaa atccaccctc atcaacggca
     6781 tgataggcaa aaaatccgcc aaaaccggca acgaacccgg catcaccaaa gccgaacaac
     6841 gccttttcct tgccgacgac ttctggctgt acgacacccc cggaatgctg tggccgaaaa
     6901 tcattgtcga agaaagcggc tacaacctcg ccgccggcgg ttcagtcgga cgcaacgcat
     6961 tggacgaaga agaagtcgcc ctcgaacttt tagactacct ccgccgccac tacctcccta
     7021 tgttgcaaga acgctaccaa gccgacaaag accccagcag ccactgggac gaaaacgttt
     7081 ggctcgaatg gatagccaaa aaacgcggcg cagtcctcag cggcggacgg atcaactacc
     7141 aaaaagccgc cgaaaacatc cttaccgact tccgtgaagg caaaatcggc agaatcaccc
     7201 tcgaaacgcc gaaccaatgg gaaacttggc tcaaaaaagc ccgtcagaaa gaagccgaac
     7261 tcaaagccat acgcgaagcc agaaaagcag agcgtaaagg gcagaagcct gcggacgagt
     7321 agggcggtat agtggaatgc caaaagaggt ttcactatct tcaaacaggt cgtctgaaac
     7381 ctcagaataa atttttcaga cgacctgttt gtaaaattta aaaccaacat atatagtgga
     7441 ttaactttaa accagtacgg cgttgccttg ccgtactatc tgtactgtct gcggcttcgt
     7501 cgccttgtcc tgatttaaag ttaatccact ataaaactga aagaaccctt atccaaggct
     7561 gtctgaaaaa tatttaaact attaaatccg gatgttatga ctgaaaaact ccctcctgat
     7621 gcgctgattg aagccgcgct tttgactcaa gccgaacctt taaacgaaaa gaccatgcgc
     7681 gaattgtgtg tgccgccgtt gtcgcaagac aaactgattg atgtgttggc gcagttgaaa
     7741 acgcgttggc aggatagggc gttgcaactg gtgcatacgc aagagggctg gcgttttcag
     7801 attgttcaga cggcattcga gcggctgggc agcctgcaag aacagcgtgc gccgcgctac
     7861 tcccgcgccg tgatggaaac actggcgatt atcgcctacc agcagcccgt aacgcgcggc
     7921 gacatcgagg gcatacgcgg cgtggcggtg tcgcagaacg tgatgcagac cttgcaggat
     7981 cgggggtgga ttgaagtcat cggacatcgg gacacattgg gaaaacccgc attgtgggca
     8041 acaacggcaa cgtttctcag cgatttgcgc ttggacggtc tggaagaact tccgccgctg
     8101 accgaactgg gcgaactggt tttacccgat ttaatggaaa tgccgcctac ggatgaagaa
     8161 gagccggaag ccgtaccgtc cgataccctg cccaactgaa attccaaatg ccgtctgaaa
     8221 cgcacattgc ttcagacggc attgcaacaa ataagcagat aaaaacaagt actaagcaaa
     8281 attaaggaaa acttattttt aatttaaaaa aatcttagtt ataattcgta tatctaaagt
     8341 tgatattgct tttgtcggta gaattgctaa ggaatcctca caatgcttct aacactttct
     8401 ttgcgtgatt ttgtcattgt tgaaaatctg aatctggatt ttcaaagcgg ctttaccgta
     8461 ttgaccggag aaaccggcgc gggcaagtcc attactttgg atgcgattgg tctgctgttg
     8521 ggcgacaaag ccgattacag ccaagtccgc agcggtgcaa aagaagcgca gttgtcggca
     8581 ttgttcgata tttcccattt gcctgtttta aaagcagaat tgtatgaaca ggggctttta
     8641 aacgacggag aagaagaact cagtatccgc cgcattatcg atgccaaagg caaaagccgc
     8701 agctttatca acaatcaggc cgctaccttg gcgcaactca aatccgtcgg tagccagctt
     8761 atcgacatcc acgggcaaaa cgcccatcat tcgcttaatc aggaagccgc ccagcgcgaa
     8821 ttgttggacg catttgcggg tagcagggag caggcggaaa ccgtcaggca gctttatcaa
     8881 aattgggcca atgcgaaaaa agccctccaa gaggcgcagg aacacgccga tgccgtcatt
     8941 atcgagcggg agcgtctgga atggcagttt aacgaactga atcagttgga cattaaacaa
     9001 ggcgaatggg aagccctcag ccaaagccac gacagccttg cccattctgc cgagctgttg
     9061 caggctgccg aagaagtcgg aagcaagatt gacggcgaca acggcatcca acgccatatc
     9121 tatcaatgtc aaaaactatt ggccaatctg caaaacatcg agccgcgctt tgccgagagc
     9181 ctgaatatgt tggcaagcat cgaagccgaa ttgggcgaaa tcagtgccaa tatgcgcgat
     9241 gtggcaggtc gcagcgacat caatcccaat gaattggcgg cacaggaaca gcgcatgggc
     9301 gaactgatgg ggatggcgcg gaaataccgg atcgagcctg aagagttgcc tgccaagttg
     9361 gcagaaatcg aagaacgcct gcaaagcctg caagctgccg ccgatttgga cgcgctcgag
     9421 cataatgttg cccacaattt tgccgaatat caggaagctg cccacatcct ttctgccatg
     9481 cgccatcagg cggcagagcg tttgagcggc gaaacgaccg agcatatgca acaccttgcc
     9541 atgaaaggtg cacgtttcga catcgtcctg ttgccctcgt cgccgacggc acatggtttg
     9601 gagcaggttc aatttcaagt tgccgccaac aaaggcaatc cgccccgtcc gctgaataaa
     9661 gttgcctccg gcggcgaatt ggcgcgtatc agccttgcct tacaggttgt tgccagccaa
     9721 tatacccaag ttcccaccct gatttttgat gaggtcgata ccggtattgg agggggagtg
     9781 gctgaaatgg tcggcaaggc attacgttcg ttgggcagaa aacatcaggt gcttgccgtt
     9841 acccatcttc cgcaagtcgc atcctgcggc gaaaaccact ggcgggtgcg caagcacagc
     9901 gagggagagc aaaccgtcag cgaaatcagt atattggata aaatccaacg ggtcgaagag
     9961 gttgcccgta tgttgggcgg agaagtcatt accgatacga cgcggcaaca tgcggcagaa
    10021 ttgctgcaac ttgcgtcgaa aaatagttta ttttaaaatc aatcagttaa aaaataacta
    10081 aaaataaaag tctaaaacaa tagacagaac tcagataaat ccgtattatc acgctttctt
    10141 aatcacttga acaagtgatt gtgctgcacc cgtagctcag ttggatagag tatctggcta
    10201 cgaaccagag ggtcgggcgt tcgaatcgct ccgggtgcgc cagtaagaaa atacaatatg
    10261 cgcccatcgt ctagcggtta ggacatcgcc ctttcacggc ggtaaccggg gttcgattcc
    10321 ccgtgggcgt gccaattcaa aatgcctccg attatatcgg aggcatttct catttctcat
    10381 ttctcatttc tcatttctca tttctcattt ctcat
//
LOCUS       DAXHPG010000060        10388 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXHPG010000060 DAXHPG010000000
VERSION     DAXHPG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.060"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(99..1190)
                     /locus_tag="ACFM6F_001139"
                     /pseudo
     CDS             complement(99..1190)
                     /locus_tag="ACFM6F_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            complement(1324..1872)
                     /locus_tag="ACFM6F_001140"
     CDS             complement(1324..1872)
                     /locus_tag="ACFM6F_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6164558.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            complement(2027..2398)
                     /locus_tag="ACFM6F_001141"
     CDS             complement(2027..2398)
                     /locus_tag="ACFM6F_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC6164559.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            complement(2454..2561)
                     /locus_tag="ACFM6F_001142"
                     /pseudo
     CDS             complement(2454..2561)
                     /locus_tag="ACFM6F_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2693..4384)
                     /gene="dld"
                     /locus_tag="ACFM6F_001143"
     CDS             complement(2693..4384)
                     /gene="dld"
                     /locus_tag="ACFM6F_001143"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC6164560.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGRALAVV
                     RPGTILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHGGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLTSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            complement(4552..4648)
                     /gene="ffs"
                     /locus_tag="ACFM6F_001144"
     ncRNA           complement(4552..4648)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFM6F_001144"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4911..8744
                     /locus_tag="ACFM6F_001145"
     CDS             4911..8744
                     /locus_tag="ACFM6F_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC6164561.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVGRFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEFVELAGLDGKHPIIHCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGRNIVNTKRLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAISPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWDWLLANLDTPLADYKARYGAA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIEGLGKIHAKTV
                     RGRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVQGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYTDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            8820..9821
                     /locus_tag="ACFM6F_001146"
     CDS             8820..9821
                     /locus_tag="ACFM6F_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC6164562.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(9883..10065)
                     /locus_tag="ACFM6F_001147"
     CDS             complement(9883..10065)
                     /locus_tag="ACFM6F_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164563.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            10252..>10388
                     /locus_tag="ACFM6F_001148"
     CDS             10252..>10388
                     /locus_tag="ACFM6F_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6164564.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YL"
BASE COUNT         2351 a         2845 c         2830 g         2362 t
ORIGIN      
        1 gggtttctgt tttttccgat ggattcctgt tgcgttgggg gctggattcc cgctttcgcg
       61 ggaatgacgc ggtgggggtt tcggtttttt ccgcctgttt attttgcggc ttcgattgcc
      121 gctatttctt tgcgtaggtg tttgattgcg ggggttacga tggcgacaaa catcgcttcg
      181 cggacgcgcc tttgggcggg actgcgcatc aggtagcctt ttgcgccgga atgcagtgcg
      241 gcggattggg cggcggcaag tgccagtacg gcggcggctt cgcgcagctt gagggtagca
      301 aggttgtcgg gcgtgccgct ccaagccaag ccggcgagcc gttcggtttc tgcccacgcg
      361 ccgtccaacc ttgctttgag gctgtcgtag ccgtcgtcga gatagctgtt gacttctgca
      421 ttgacgatgt tggcgaggcg gatgatgccg aggctgccgt ctatcacgcc cgcgccgatg
      481 ccgatttgca ggaggataaa gcctgctttg atgctttgga tgtagtcggc aaactgttcg
      541 ggcgcggcga tgatgtcttc gtcggggata aatacgtctt tgaagttcag gctgaaggtg
      601 cgcgtgcctt cgagggcgca aaattcgggg cagttttgca ggcttacgcc ttcccattgt
      661 ccgccggtga taaacataac gtaaccgtcg ccgatttggg cggtattcgc ctagatgtgg
      721 tcttcgccga tgttggacac ccacggcagc gcgccgttga ctgtgtagcc gccctccact
      781 cgctcagctt ggaggttgtg tttttcgatg ccggcaaggt gtttgacggt attggacatg
      841 cccgtacccg ccaatacttt gccttgcagg atgtcggcga ggtatttgtc tttgacggct
      901 tggttgggcg tttggtgcag ataccacgcg caagccgcct gacaccacgc gctgaaggag
      961 gttgcgccgc attctttgcc gatttcgcgc aagacggcga tttgcgttgc caaacccaag
     1021 ccgttgccgc cttcggcttc tgtgccgact gcgccgaatc cgccgattgc gccgagttcg
     1081 cgcataaatg cttcggggta gtatcctttg cggtcgatgt cgtccactaa gggtttgagc
     1141 ttggttttga cgagttcggc aacattggca atcagggttt gggcgttcat ctttgttcct
     1201 taaggtttgc gggggaatcg ggggcgcgcc tgatgcgcgg ctgcttccct gccgcttggc
     1261 ggcttgtttt tgtttgaggg ggtgtttttg aaaaaccgcc cgatattcgg gcggtttgcc
     1321 gtatcaggcg taagcctgca attcggggtt gatttcggtt tgtccgaggt tgttgacgta
     1381 gttgcacagg gttgccaagg ctacgcccat cacgacttcg actgcctgct gctggttgta
     1441 gcccgcatcg aaaaatgctt tgagttcctc gtcggatacc gcgccttttt tcgccattac
     1501 ggcttgggtg aaggcggcga gcgcgccgag tttggcatcg tcaaattcgc ctgctgccaa
     1561 agcgcgcgcg gctttgacgg attgttcgga caggagtttt ttcagggttg cgagtttggt
     1621 gtgccctgcc acgcaaaaac cgcattggtt ggtacgggcg gcgatgatct ggatgacttc
     1681 gacttcgcca gcggtcaggc tgttggcggc gttgagcttg ccgacttctt ggtaaaacgc
     1741 caaggcttcg ggggcgtttg ccaatacgcc gataagattg gggataaagc cgttgttttg
     1801 aagtaccgcc tcgacgcgcg ctttggcggc ttcgggggcg gtttcgaggg tgtgtacggt
     1861 taaacgtgcc attttctttc cttgttcgca aatattgggt acgggcgcat ggtatgcatt
     1921 tcggaacgga ataggaaaga ctgattggtt atgtgctgca aacaaaaggt tataagaaat
     1981 gccgtctgaa catttttcag acggcatgat ggaaaagaaa acgcgcttat cggcccccgc
     2041 gcccgaaata ttgcgccaat gcggcttggg gattggctgc ctcaaccgtt tcgggcaatt
     2101 cggtatagcc gcgcgataaa aggtggcgtg cataaaggct gttgcccttt gcgccgaacc
     2161 agacggaaac cgccaagccc aatacgttaa acgccgagtc tgtggcgggc gtgtcgccgt
     2221 aaaccagttg ggcaaacacc gccagcacga atatcgcgcc cgtcagcccc agccccaaac
     2281 cccacattct tttgaaacac gcccacagcg tgccgaacaa aaggcccggc cacgaccagc
     2341 cttgtttgac ggcttggggc ggcagggcgg gatgggtgta gattttgtat ggtttcatcg
     2401 tgtttccttt tcggttgaaa ccctgccctt tgggaaggta ggatcagact ttatagtgga
     2461 ttaaatttaa accagtacgg cgttacctcg ccttgccgta ctatctgtac tgtctgcggc
     2521 ttcgttgcct tgtcctgatt taaatttaat ccactatatt tgggaggcgc gcgcgcctgt
     2581 gccggcatac ggcttgaaag cgattacccg atggggaact tcaaacccga caatgccgtc
     2641 tgaacggcgt tttgccttca gacggcattg cctgccttca aagcggacgc gcttattccg
     2701 cccagttttt ctttttgctg gttttgccta cgcccgggtt gaagctgttg gtcgggtcaa
     2761 gtttgcggta aaactgtttg agcgcgggtt tggcttcata caaatgaccg acgttgtgtt
     2821 cggcgggata ttgcgcgccg cgttggtcta aaagatgcag catttcgtgt tccaacgcca
     2881 tgcagtcgtt gccttttttg atgatgtaat cctgatggaa aacgtggcac atgaaatgtc
     2941 catagtagag tttatggatg attttattgt cgatttccga cgtcagtttt tcaaaccagt
     3001 cgcgatcgtc gcggcgcagg gcgatgtcga gcgcgaccaa gtcttccact tcgtcatcat
     3061 gcacggcgcg gtagcggatg gcggcggaag cgacggcgaa acggtgcagc atcgcggctt
     3121 gggtttcttc ggcgttgcac tcgaaaaacg cgccgccgtg gtgtgcaaaa tattctttta
     3181 agaacgcgcg cgcctcatcc acgcctttcc cacccatttt cagaatcaga tggtgttcgt
     3241 atttgtcgcg gtaatcgcgc atggatttgg gcaggtggtc gggcaggaat ttgctgacga
     3301 actgcatggc cttgtcggaa aaatgtttgg gcaggaagct gacttttttg ccgaacctgt
     3361 ccacgcgcgc cttcaagtca aataatttcg gcagttggtg cgtaccgaat tttttgatga
     3421 cgtaaaacgt atctttgccg tacacgtcgg caatatcgaa agcgtggcgg tggatgtatt
     3481 cgccggaaac gggcaggctt tcaaattcgc ccaaggcggc gcggcggatg tcggtcagtt
     3541 cgttgatgtc attcgtgccg atgtagaaca cggcagtttg tttttcttgc gggaaggtgt
     3601 ccaaacggac ggcaaaaacc atcagtttgc ccgcgcagcc cgaggcttcg taatggcggg
     3661 cggggtcggc attgaaacgc gcggcggtcg gttcgtccac ttggcggacg tgttcgcaat
     3721 aggcgtggtc gtgccctttg cccgcgtctt gtttgatgtc tttgttttga taatgatgac
     3781 cttgcaggtt ggtcaggatt tcttcgggcg tgtcgcccaa gtctatgccc aagtggttga
     3841 ccagttccaa cctgccttct tcgttgattt gggcgaacag tgccatttcg gtgtaggccg
     3901 ggccgcgctg caccaatgcg ccgccggagt tgttgcacac gccgcccaag acggacgcac
     3961 cgatgcagga agagccgata accgaatgcg gttcgcgccc caaaggtttc agcagcaatt
     4021 caagctggtt cagggtcgag ccgggcaggc agacgacttg ttcgttgttg ttgatggttt
     4081 ggatgatgtt catccgcatg gtgttcacaa tcacgatgtc gcggtcgtaa tcgttgccgt
     4141 cgggggtcga gccgcccgtc aaaccggtat tcgccgcctg cgtaatcaca atcacgtccg
     4201 cttcgacgca ggcctgcaga attttccaca tttccagaat ggttccgggg cgcaccaccg
     4261 ccaacgccct gccctcgccg aagcggtagc cttggcggta ttgttcggtc ttcgcggggt
     4321 cggtgatgat gtatttttcg cctacggttt gggtcagtct tgacagtaat tgtgatgcgc
     4381 tcatggcagt ttccttaaaa ttgtcggcag gtgcatatgc acattgaaat tgttttccca
     4441 ttgtagttat gcgttatggc aaagtaaagg aaatgccgtc tgaacggctt tcagacggca
     4501 tcggtgcgat acgggaacgc cggaacatcg aagctccggc gtttcaaata gggcggcggg
     4561 ccaaaccccc ggcactggcg catcggagtg ggctgctggc ttccgcccct gacccggtgt
     4621 tccgatttgc catgcgggga gacccgcctc aagaagccgc acattatagc ggtttttggg
     4681 aaaaactcaa ccgttttgat gcagtcatac gccggaaacg gcatctgaaa tttatcatcc
     4741 gataatattg tcaacaattt ttcaaagcgt cattttgttt ctataagggt atttcccatt
     4801 ccgacattga aaaatattaa aaattgaact acattatcgc cttttcaaac ccgcctgaaa
     4861 ccgacttttc agacggcatt caaataaaaa ctgccaaaca cggacacacc atgaccacga
     4921 ctactgcccc tcagcgtatt cgagaaattc cctataacta tacttcttat accgaccgcg
     4981 aaatcgtcat ccgcctgctg ggcgacgaag cgtggcagat tctgcaagac ttgcgcggac
     5041 agcgtaaaac cggacgttcg gcacggatgc tgtttgaagt gttgggcgat atttgggtag
     5101 ttgtgcgcaa tccatatctg gtcgatgact tgttggagca cccgaaacgc cgcgccgcgc
     5161 tggtgcggga aatgcgccac cgtttgaacg aaatccgcaa acgccgcgac gacaaccgcc
     5221 aagtcgatgt cttggttgcg gcggcggaaa aggcggtcgg gcgttttgat agcagttttg
     5281 atgaaaccag ccaaaagcgg cggcagattt tggagcgttt gagcaaaatc accaagccgc
     5341 acaatattat gtttgacggg ctggcgcggg taacgcacgt taccgatgca accgactggc
     5401 gcgtggagta tccgtttgtc gtcgtcaatc ccgacacgga ggcggaagtc gcgcctttgg
     5461 tgcgcgcctt aattgagttg gacttggtta ttatcccgcg cggcggcggt acgggttata
     5521 ccggcggcgc gattcctttg gacgcaatga gcgcggtcat caatacggaa aagctggaca
     5581 aacatcgcgg cgtggagttt gtcgaactgg cgggcttgga tggcaaacat ccgattatcc
     5641 attgcggcgc gggcgtggta acgcggcggg tagaagaaac cgcgcatcag gcgggtttgg
     5701 tgttcgccgt cgatccgact tccgccgatg cgtcctgtgt gggcggcaat gtggcgatga
     5761 acgcgggcgg caaaaaagcc gtgctgtggg ggacggcgtt ggacaacctc gcctactgga
     5821 acatggttaa ccctcaaggc gaatggctgc gtatcgagcg cgtgcgccat aatttcggca
     5881 aaatccacga cgaagaaacc gccgtgttcg acgtacatac gctggattca gacggccgca
     5941 atatcgtcaa taccaaacgt ttggaaatcc ccggtcacaa gttccgcaaa gtcggtttgg
     6001 gcaaagacgt taccgacaaa ttcttgagcg gactgcccgg cgtgcagaaa gaaggcacag
     6061 acggcatcat caccagcgtt gccttcgtat tgcacaaaat gccgaaatac acgcgcaccg
     6121 tgtgtatgga gtttttcggc acggttgcca ccgccacgcc atctattgtc gaaatccgcg
     6181 actttttgct tgcccatgaa agcgtacggc tggcgggttt ggaacatttg gactggcgtt
     6241 acgtccgtgc cgtcggttat gccaccaaag cagcaggcaa ggggcggccg aaaatggttt
     6301 tgctggcgga cgtggtttca gacgacgaag ccgccgtaga ggcagccgcc gaacacatct
     6361 gcgaacttgc ccgcgcccgc gacggcgaag gctttatcgc catatctccc gaagcccgca
     6421 aaaccttctg gctcgaccgc agccgcaccg ccgccatcgc caaacacacc aacgccttta
     6481 aaatcaacga agacgtggtc atcccactcg aaaggctcgg cgagtattca gacggcatcg
     6541 aacgcatcaa cattgagctt tccattcaaa acaaactcaa actctgtgcc gccttggagc
     6601 aatatctgtc cggcaaactc cccatcgaca aaatgggaac cgacctgccg accgccgaac
     6661 tgttgggcga acgcggcaaa cacgccctgg cccacgtttc cgccgtcaaa gcgcgttggg
     6721 actggttgct cgccaatctc gacacgccgc ttgccgacta caaggcccgc tacggcgcag
     6781 ccgtccacgc cgcgcccgaa gccaaagaca atgaaagctg ctttaccgca ttccgcgatt
     6841 tccgcctgcg cgtgtctgtc aaagcagacg taatgaagcc gctttctgaa atcttcagcg
     6901 gcaaaaccga caccaaaatc atcgaaggct tgggcaaaat ccacgccaaa accgtgcgcg
     6961 gcagagtctt tgtcgccctg catatgcacg ccggcgacgg caatgtccac accaatatcc
     7021 ccgtcaattc agacgatgcc gaaatgcttc agacggcata ccgttctgtg gaacgcatta
     7081 tgaaaatcgc ccgttcgctt aacggcgtga tttcgggcga acacggcatc ggcatcacca
     7141 agctcgaatt tttaagcgat gaagaaatgc agccgttttg gaactacaaa aaccaagtcg
     7201 atcccaaaca caccttcaac cgccacaaac tgatgaaagg atcggactta cgcaacgcct
     7261 acacgccgtc gttcgagctt ttgggtgcgg aatcgctgat tatggaaaaa tcagatctcg
     7321 gcacgattgc cgattccgtc aaagactgcc tgcgctgcgg caaatgcaaa cccgtctgct
     7381 ctactcacgt tccgcgtgcc aacctgctgt acagtccgcg caacaaaatc ctcggcgtgg
     7441 gcttgctgac cgaagccttc ttatacgaag aacaaacccg ccgcggcgtt tccgtcaaac
     7501 actttgaaga actcatggac atcggcgacc actgcaccgt gtgccaccgc tgcgtcaaac
     7561 cctgccccgt caacatcgac ttcggcgacg taaccgtagc cgtccgcaac taccttgccg
     7621 attccggcca caaacgattt gcgcctgccg cagctatggg tatggcgttt ttgaacgcca
     7681 ccggcccgaa aaccatcaaa gcccttcgcg ccgccatgat acagaccggc ttcccagcgc
     7741 aaaactttgc ctacaaaatc ggcaagcttc ttccgatcgg cacgaaaaag caaaaagccg
     7801 aacccaaggc aaccgtcggc aaagccccaa ttaaagaaca ggttatccat ttcatcaacc
     7861 gtcctttgcc gaaaagcgta cccgccaaaa caccgcgctc cttattgggc atcgaagacg
     7921 gcaaaagcat ccccatcatc cgcaaccccg ccgcgcccga agatgccgaa gccgtgttct
     7981 acttcccggg ttgcggttcg gaacgcctgt tcagccaaat cggacttgcc gttcaagcca
     8041 tgctctggca cgtcggcgta caaaccgtcc tgccgcccgg ctatatgtgt tgcggctatc
     8101 cgcaagacgc gggcggcaac aaggctaaag ccgaagaaat gagcaccaac aaccgcgtgg
     8161 ctttccaccg tatggcaaac accctcaact acctcgacat taagaccgtc gttgtcagtt
     8221 gcggcacttg ttacgaccag ctcgaaaaat accgctttga agaaatcttc cccggctgcc
     8281 gcatcatcga catccacgaa tacctgctcg aaaaaggcgt gaaactcgac ggcgtgcaag
     8341 gtcagcaata cctctaccac gacccctgcc acacccccat caaaaccatg aacgccaccc
     8401 aaatggccag cagcctgatg gggcagaaag tcgtcttaag cgaccgctgc tgcggcgagt
     8461 ccggtatgtt cgccgtcaaa cgacccgata tcgccaccca agtcaaattc cgcaaacaag
     8521 aggaaatcga gaaaaacctc aaagagctgc cgcagggtga acccgtcaaa atgcttactt
     8581 cctgcccagc ctgcctgcaa ggcttgagcc gctacaccga cgacaacaat atgcctgccg
     8641 actacatcgt catcgaaatg gcgaaacaca tcctcggcga aaactggcta gacgagtttg
     8701 tgaaaaaagc caacaacggc ggcgtggaaa aagtattgct gtaacaacgg acacggaaat
     8761 gccgtctgaa cgccgaagtc ttcagacggc attgtttaaa cccaatacct acccaaatca
     8821 tgattgacgg acaaacaacc gaacccaaac aaaaaacccg aatcatcctt gcccctatgc
     8881 agggtctggt cgatgacgtg atgcgcgacc tgctgacgcg tattggcggc tacgacgaat
     8941 gcgtcagcga atttgtacgc attacccata ccgtgcattc ccgatccata tggttaaaat
     9001 atgtccccga aatcgccaac ggaaacaaaa cgttttccgg cacgccctgc accgtccaac
     9061 ttttgggcag cgatgcggac aatatggcgg cgaatgcgct ggaagccgtc cgcttcggtg
     9121 cgaacaaaat cgatttgaac ttcggctgcc ccgcgcccac cgtcaacaaa cacaaaggcg
     9181 gcgcaatcct tttaaaagag ccggaactga tattccacat cgtcaaaacg ctgcgcggac
     9241 gtttgcccgc acatattccg ctcaccgcaa aaatgcggct cggttacgaa gacaaaagcc
     9301 gggctttgga atgcgcctgt gcgattgccg aagggggcgc atgcggactg accgtacacg
     9361 cgcgtaccaa agccgagggt tacgaaccgc cggcgcattg ggaatggata aggaaaatcc
     9421 gagacagcgt caatattccc gttaccgcca acggcgacgt tttcagcctg caagactata
     9481 tcggcatcaa aacaatcagc ggctgcaaca gcgtgatgct cggtcgcggc gcggtcatcc
     9541 gccccgattt ggcgcggcaa atcaagcaat acgagaacgg cgggccggtc aaagacacgg
     9601 attttgccga agtttccaaa tggatacggc agtttttcga gctgtgcctg acaaaagagg
     9661 caaacaataa atacccgatc gcccgtctta agcaatggtt gggcatgatg aaaaaaacgt
     9721 tcgacccggc gcaaacgctg tttgaccgcg tccgaacggt taaggatgcg gatgaagtcc
     9781 gccgaatcct gaacgcattt gagcatgaga taaatgtttg actgtctccc atgcttcaaa
     9841 gcaaatgccg tctgaaaatc tttcagacgg catttcatat ctttattgct gcttttcttc
     9901 cgtatccggc gtatttttgt ttttcagctt cgcacccaag cccaaacgtt tttcataatc
     9961 cgattgcggg gtatcgtctt cctgcatacc gaatgcgccg acattgaccc acagcgacag
    10021 cagcgcgacg acaaaggagc aaaagccgat tacataccaa aacattgccc ctcccgattt
    10081 gttaaaatca tatcaaatac agtgccgaat ttatcacaaa cgcacgggca aatatagtga
    10141 attaaattta aataaggaca aggcggcgag ccgaagacag tacaaataga gacctttgca
    10201 aaattcccca aaatccccta aattcccacc aagccattta ggggattttc catgagcacc
    10261 ttcttccggc aaaccgcaca agccatgatt gccaaacaca tcgaccgttt cccgctattg
    10321 aagttggacc aggtaattga ttggcagccg atcgaacaat acctgaaccg tcaaagaacc
    10381 cgttacct
//
LOCUS       DAXHPG010000061        10352 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXHPG010000061 DAXHPG010000000
VERSION     DAXHPG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.061"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..141
                     /locus_tag="ACFM6F_001149"
     CDS             <1..141
                     /locus_tag="ACFM6F_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HFC6164565.1"
                     /translation="VGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRHI
                     RT"
     gene            270..1706
                     /locus_tag="ACFM6F_001150"
     CDS             270..1706
                     /locus_tag="ACFM6F_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC6164566.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLSFVVFNTASPD
                     DAGAVFNALFANANGWNAPQQANMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1717..2700
                     /gene="patB"
                     /locus_tag="ACFM6F_001151"
     CDS             1717..2700
                     /gene="patB"
                     /locus_tag="ACFM6F_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC6164567.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYTFSDGIKAFLSGETPSTAQGGGSADMPSEAAASEAVPQTGKT
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2697..3890
                     /locus_tag="ACFM6F_001152"
     CDS             2697..3890
                     /locus_tag="ACFM6F_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC6164568.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKIRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(3950..4126)
                     /locus_tag="ACFM6F_001153"
     CDS             complement(3950..4126)
                     /locus_tag="ACFM6F_001153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164569.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAVCRRFACVLPMPEANAGCI
                     WQTVRGVAMPSEAI"
     gene            4177..5730
                     /locus_tag="ACFM6F_001154"
     CDS             4177..5730
                     /locus_tag="ACFM6F_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC6164570.1"
                     /translation="MNRTYANFYEMLTTACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASVGLSKELAGLKAQTPIEKIIWTGKSRPVGETAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(5821..7848)
                     /locus_tag="ACFM6F_001155"
     CDS             complement(5821..7848)
                     /locus_tag="ACFM6F_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC6164571.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFGWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            8137..10143
                     /locus_tag="ACFM6F_001156"
     CDS             8137..10143
                     /locus_tag="ACFM6F_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC6164572.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLTIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            10285..>10352
                     /locus_tag="ACFM6F_001157"
     CDS             10285..>10352
                     /locus_tag="ACFM6F_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6164573.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAP"
BASE COUNT         2491 a         3225 c         2500 g         2136 t
ORIGIN      
        1 gtcggtatcg gcagcggcgc actgttggga cattgggtaa cgcaatatgt cggtatttcc
       61 cgtatcggca tcgccggtat gcttgcggcg gcggccgggc tgtggctgtg cctgaacttg
      121 aaccgccata tccgaacctg acccgctatg ccgtctaaag cctctcccgc ccttcagacg
      181 gcattttgat tgaagaaata tccgcccctg cttaaaataa cgggctttgc cgtgttttaa
      241 cgcctatttt cttattccaa ggatggttta tgccgctgct gtctgtcgag ttcgcactgt
      301 tctttctcgc cttcctgccg atttactggg gcttggcgaa atacccgtcc gtccaaaacc
      361 tgctgctttt ggctgccggt atgggctggc tctaccatat cggccccgta tttgcggcaa
      421 tcgtcgtcct ttattcctcc tgcgtgtacc ttttgggcga actgctccgt tccgatcgcg
      481 aaaatacgcg ccgcttttgg ctggggtgcg gcattgccgt ctcgctgacc gtcttgggct
      541 ttttcaaata tttcgacttt ttccgcccgc tgattgcaca atatgccgga caaggcggcg
      601 caatcgacat cctgatgccg ctggggcttt cgtattacac cttccagtcg ctcgcctatc
      661 tggtttactg cttccgcgcc ccgcacgccg cgcgtttcga gtggcacgag ctgctgctgc
      721 acctgagttt tttccccacc gttacctccg gcccgattat ccgcgccgcc gcattcaaaa
      781 gcgcagacgg cgagcaggcg ggcgcattgg cgcaaatccg tacccgccaa ccgcgctcac
      841 ccgtccgccc cgcactcgcc gtttccctga ttctgctggg tattgccaaa aaatggtggc
      901 tggcggggat gctggcggaa aactgggtgt cgcccgtatt tgaaaatccc gcccaattcg
      961 acggctgggg cgtattgggc ggcgtgtacg gctatacctt ccaactcttt ttagactttt
     1021 ccggatattc cgatttggtt atcggcatgg cgatgctgct gggtttccgg ctgcccaaaa
     1081 acttctccgc accgcttcgt gctttaaaca tccgcgcatt ttgggacaaa tggcacatca
     1141 gcctttccac ctggatacgc gactacatct acatcccctt gggcggcagc aaaaaaggct
     1201 ttttacggac acagctcaac ctgatggcgg caatggtact ctcaggcatc tggcacggct
     1261 acggctggaa cttcctcatt tggggcgcgc tgcacggcac ggcactggtg ctgctcaaca
     1321 cgggcgatcg ctatttcgga cgcaacgcgc tatgccgtct gaaatacctt gcgccgctct
     1381 cgtggtttgt taccttccat ttcgtctgcc ttagctttgt tgtcttcaat accgcaagcc
     1441 ccgacgatgc aggcgcagtt ttcaatgccc tctttgccaa tgccaacggc tggaatgcgc
     1501 cgcaacaggc aaacatgctg ctgcttgcct cgtttgcatc cgtgatgctg ctctaccctt
     1561 acctgcaacg cgctttcgac ggcgcggtca aaggtttgga aaaaatcccg atgtggctgt
     1621 ggtttatccc cgtttccgcc gtcctgctgc tgattatcgt attggccccc tcggggattc
     1681 ccggctttat ttatgccaat ttttaagggt ttggacatga aaaactttct ttcccttttc
     1741 gcctccatac tgatgtctgc cctgattgcc gtgtggttca gccaaaaccc catcaacgcc
     1801 tactggcagc agacctacca ccgcaacagt ccgctcgaac cgcttgccgc ctacggatgg
     1861 tggcggagcg gtgcggcgtt gcaagaaaac gcctacacct tttcagacgg catcaaagcc
     1921 ttcctgtccg gcgaaacgcc gtcgacggct caaggcggcg gttcggcaga tatgccgtct
     1981 gaagccgccg catcagaagc cgtccctcaa accggtaaaa cagaatggaa acaagacacc
     2041 gaagccgccg ccgtccgcag cggcgacaaa gtcttttttg ccggcgactc gctgatgcag
     2101 ggcgttgcac ccttcgtgca aaaaagcctg aaacagcaat acggcatcga atccgtcaac
     2161 ctcagcaaac aaagcacggg gctgtcctac ccctcattct tcgactggcc gaaaacgatt
     2221 gaagaaaccc tgaaaaaaca tcccgaaatc agcgtgctcg ccgtcttcct cggcccgaac
     2281 gacccgtggg atttccccgt cggcaaacga tacctcaaat tcgcttccga cgaatgggcg
     2341 caagaatacc tgaaacgcgt cgaccgcatc cttgaagccg cacacacgca ccgcgtccaa
     2401 gtcgtctggc tcggcatccc ctatatgaaa aaagccaagc tcgacgggca gatgcgctac
     2461 ctcgacaaac tgctttcgga acacctggaa ggcaaaatca tcctgattcc caccgcgcac
     2521 accctgagcg gcggggaaga ccgctacacc gactccgtca acgtcaacgg caaacccgtc
     2581 cgctaccgca gcaaggacgg catacacttt accgccgaag gacaaaaact gctggcggca
     2641 aaaataatgg aaaaaatcgt ttttgaacca agtacgcaac catcaagtac acagccatga
     2701 accccaaaca cctcatcgca ttttccgccc tgttcgccgc cacgcaggca gaagccctgc
     2761 ccgtcgcctc cgtcagcctc gacaccgtta ccgtttcccc gtccgccccc tacaccgata
     2821 caaacgggct cttgaccgac tacggcaacg ccgccgcctc gccttggatg aaaaaaatcc
     2881 gatccgtcgc acaaggcagc ggcgagacct tccgcatcct gcaaatcggc gactcgcata
     2941 ccgccggcga cttctttacc gacagcctgc gcaaacgcct gcaaaaaact tggggcgacg
     3001 gcggcatagg ctgggtttac cccgccaacg tcaaagggca gcgcatggcg gcagtccggc
     3061 acaacggcaa ctggcaaagc ctcaccagca ggaacaatag cggagacttc ccgctcggcg
     3121 gcatcctcgc ccacaccggc agcggcggca gcatgaccct gaccgcatcg gacggcaaga
     3181 ccggcaaaca gcgcatttcc ctgtttgcca aacccctgct cgccgaacaa accctgaccg
     3241 tcaacggcaa caccgtctcc gccaacggcg gcggctggca ggtactggat acccgcgcgg
     3301 cactgccctt gaccatacag accgaaatgc cgtgggacat cggcttcatc aacatcgaaa
     3361 atcccgccgg cggcattacc gtttccgcca tgggcatcaa cggcgcacaa ttgacccatt
     3421 ggtcgaaatg gcgtgccgac cgtatgaacg accttgccca aaccggcgcc gatttggtta
     3481 tcctttccta cggcaccaac gaagccttcg gcgacaacat cgacattgcc gacaccgaac
     3541 aaaaatggct ggataccgtc cgccaaatcc gcgacagcct gcctgccgcc ggcatcctca
     3601 tcatcggcgc acccgaatcc ctgaaaaaca cgctcggcgt atgcggcaca cgccccgtcc
     3661 gcctgaccga agtccaacag atgcagcggc gcgtcgcccg acaggggcag acgatgttct
     3721 ggtcttggca aaacgccatg ggcggcatat gcagcatgaa aaactggctc aaccaaggat
     3781 gggccgccaa agacggcgta cacttctccg ccaaaggcta ccggcgcgcg gcggaaatgc
     3841 tcgccgacag cctcgaagaa ctcgtccgcg ccgccgcaat caggcaataa tcggacaggg
     3901 ggcggacggt atttccgcaa cagggggatg ccgtctgaaa cgcatacctt catattgctt
     3961 cagacggcat agccaccccg cgcacggttt gccagatgca accggcattc gcctcaggca
     4021 tcggaaggac gcaggcgaac ctccggcata cggcgcaaag gcggcgtttg atatgccgtc
     4081 tgaaggcaaa gatgataaac tgccgccttc cgttttcaga cggcatattg ttttcaaatg
     4141 agggcgttcg ccgtccgcaa ccataaagga agtttcatga accggactta tgccaatttc
     4201 tacgaaatgc tgaccaccgc ctgccgcaaa aacggcaacg gcacggcagt gttcgacggc
     4261 aaggaaaaaa ccgcctaccg cgcgctcaag caggaggccg aagccgtcgc ggcgtatctg
     4321 caaaatatcg gcgtgaagtt cggcgacacg gtcgcgctgg cggtttccaa ttccacagaa
     4381 tttattaccg cctatttcgc catctccgcc atcggcgcgg tcgccgtacc gatgaacaca
     4441 tttttgaaaa acagcgaata cgcgtatatc ctgaacgact gcaaggcgcg cttcctgttc
     4501 gcctcggtcg gcctgtcaaa agaattggcg ggcttgaagg cgcaaacgcc catcgaaaaa
     4561 atcatttgga cgggcaaaag ccggccggtc ggcgaaacgg cggaaggcga tgcctttttt
     4621 gaagacgtgc gccgcttccc cgaaaaaccc gacttgggcc gacaaccccg gataaatgat
     4681 ttggcacaca tcatctacac ctccggcacg acggggcatc ccaaaggcgc gctaatcagt
     4741 tacgccaacc tgttcgccaa cctgaacggc atcgaacgca tctttaaaat ttccaaacgc
     4801 gaccgcttta tcgttttcct gccgatgttc cacagcttca cgctgacggc tatggtgctg
     4861 ctgccgattt atatggcgtg ttcgattatt ttggtcaaat ccgttttccc gttttccaat
     4921 gttttgaaac agaccctgct caaacgcgcg accgtgtttt tgggcatacc cgcgatttac
     4981 accgcgatga gcaaggcgaa aatcccttgg tatttcagat ggttcaaccg cattcgcctg
     5041 tttatcagcg gcggcgcgcc tttggcggaa caaaccatcc tcgatttcaa agccaagttc
     5101 ccccgcgcca aattgctgga aggctacgga ctgagcgaag cctctcccgt cgtcgccgtc
     5161 aatacgcccg agaggcaaaa agcccgcagc gtcggcatcc ccctgcccgg tttggaagcc
     5221 aaagccgtcg atgaagaatt ggtcgaagtg ccgcgcggcg aagtgggcga actgattgtc
     5281 aggggcggtt cggtgatgcg gggctacctc aatatgcctg ccgccaccga tgaaaccatc
     5341 gtcaacggct ggttgaaaac gggcgatttc gttaccatag acgaagacgg cttcatcttt
     5401 atcgtcgacc gcaaaaaaga tttgattatt tccaaaggtc aaaatgtcta tccgcgcgag
     5461 atcgaagaag aaatctacaa actcgatgcc gtcgaagccg ccgccgtcat cggcgtgaaa
     5521 gaccgttatg ccgacgagga aatcgtcgcc ttcgtccaat tgaaggaagg tatggatttg
     5581 ggcgagaacg aaatccgccg ccacctgcgt accgtgctgg caaatttcaa aatccccaaa
     5641 caaatccact ttaaagacgg gctgccgcgc aacgctacgg gcaaggtatt gaaacgggtg
     5701 ttgaaggagc agtttgacgg aaacaaatga acgccgtgcc gtccgaagcc ccgtccggca
     5761 aaaaaaatgc ggtgaatctg cttcaccgca tttttttggc aaacgccgga gcgaggatat
     5821 ttactctgcc aattccacct gctcgtgcgc catcagttcc tgaccgacat catccaacaa
     5881 catcaaataa cgttcgtagt ttttcaaaat gtcggcaatc agctcgtcct tgttcatata
     5941 ctgcacatcg tacccgacgc gcccgtcgaa aaaataagcg tagggtttgt aagttgtctg
     6001 atgccggata tgcggcagct tgccgtcgtt aatcaactgg tcggatacat cctgcccgac
     6061 agacttaatc ccgtacataa aatcgcgcat cgtctctttc cgaatgacga actcgattgc
     6121 gggctcgtcc tgatgaaaca tcttatcgac ccggacgctc aagccgtatt cttccgaaag
     6181 ctcccgttgc aactcgtgca tagcgggcga tgcggtatgt ttgaggaatt ttaaaatatc
     6241 ctgctcctgc gtctggctca ttatctgcac cagccgttct ttccacttgc cgcccgtcca
     6301 aaatacactg gtagggttaa cccgggtctc aaaatatttc ttatccgcac tcaagccttt
     6361 ccacaggctg aaacacatta tcagcatcag cagggcaaac ggcagggaaa caatcagggt
     6421 catagactgc aggttgccga gtccgcccga gcgcatcagc aaaacggcaa cggcagacat
     6481 cagcacgccc cacataaccg cctgccaccg tggcgcgctc aagcctttgt cccgagaggt
     6541 aatattgttc aggacataaa tcccggaatc ggcagaagtt acaaaaaaca gagaaatgac
     6601 cagcaggctg acgatgctcg tcaattcggg cagggggagg taattaaaga atttaaaaag
     6661 cagcgtttcc ggagaggagg tcatcttttc gagcattccc cccgcaaccc cgtcattcag
     6721 ccaaatcgcc gtattgccga agacggtaaa ccacaaaacg ccgaacaggc cggggatgag
     6781 caaaaccccg aagacaaact cgcggatggt gcgccccttt gaaatgcgcg cgataaacaa
     6841 acccacaaac ggcgcccaag aacaccacca cgcccaataa agcaccgtcc aagattcaaa
     6901 ccacggcttg tgttcccgtt cgtacgcata agttttaaaa ctgaggcgca ccagatttcc
     6961 gaggtagttc cctatgttgt cgccgaatgc cgacaacagg taaacagtgg gacccgccgc
     7021 caaaacaaaa aacagcagca aaaacgcaag gctcaggttc aactcgctca acaccttcac
     7081 gcccttcccc acgccggata ttgccgaaac gacggcgagg gacatgacgg cggcgataat
     7141 caaaacctgc acgctgaagc tgttttcggc aatccagccc atttcctgca atccggcgcc
     7201 cagttgcgaa gccccgaacc ccaatgtggt gatgatgccg aaaaaagtag caagcaacgc
     7261 cataatatca atggcatcgc cgaaccttcc ggaaattttt tctttcaaca ggggataaaa
     7321 acaagaacgc agggcaagcg gcagcttgta gcggaaaccg aaataagcca aagccaatgc
     7381 aatcgtaccg tacaccgacc aagcgtgaac gccccaatgg aacaccgtgt gcagcaatgc
     7441 ctgctgctgc ctgtgttccg gcgtgccggc cgtaatgtcc gaaaaataat gcatcaacgg
     7501 ctctgccacg ccgaaaaaca tcagacccac gcccatcccg gccgcaaaca gcatcgccag
     7561 ccacgacagg aagccgaatt ccggcacatc ttcatcccgt ccgagcctga tgtttcccaa
     7621 actgctgacc gagagtatca gcaggaaacc cagaaaaatg gaaaacgtta aaacataaaa
     7681 ccagccgaac tcggtaaaaa tgacttcttt tgcccgatcg agccacatct gcacctgatc
     7741 cggcacggtt aaaaccaata ccaccaaaac acacacaaaa aacaaagtcg tcaaaataac
     7801 catcggatta aatgacgttc ggcgttctat aaattcagac agggacaaac cttctcactc
     7861 ctttgttaaa aacagacaaa cccggtcatc gggcaaagcg gtcaaaacct gccgacaaaa
     7921 taccggcttc cggatgacga aatgcacaaa ccggccgaaa ttgttataat cgaaaaaatt
     7981 aaaaatcaat acggattatt ctatcagatt gatttatcga tatttattat atcattaata
     8041 tagtttgatt tcaaaccggc ggaaaatgcg ccgccttccg caggcgcgcc ctccccgcca
     8101 tcggggcgtt taacgccgga ttgccgatgc ggtacaatgg ctgatatgaa gaaaattacc
     8161 cctcaaaacc tgcgccccct gctttcggaa agcttgggac ataccgattt tgtcaacgtc
     8221 ctcaacgcac tgattaaatt tttgcgccgt ggcggcaaaa aatgtgcggg ggagcgtttc
     8281 gacctgatta tcgacacatt caaacaagac agggaattgc tgtcccgctt cagccggtgt
     8341 ttttacatct ggctcgcgca aatacacatt tatccggcac tcatcaaact cggcatcttc
     8401 tcgcgccaca gctttgcccg agaaatgggc atacgcatct acgaacgctt cagcccgtca
     8461 tataaagatt ttgccaactt gggcgaagtc ttcctttatc ttttccattc cgaaaacgac
     8521 gacaaatggc tgcaaacgct caatatccgc caatggctgg ttttatacga actcatccgg
     8581 agccacgccg agccgtccaa attgcagacg gcgggcatcc gccttgccga tgcgcgtttg
     8641 cgcgccatcg aaatgctgtc tgtctggacg gcatccgaag ccatcgaacc cgacctcatc
     8701 cgcatcgccc cgcgcctgct ggaagccgat tcttccttcg tcgccctcca acgcgaaacc
     8761 gccaaactgg tcgaacacta ccgcaacggc accacgcctt acgacaccgc ccacctcgaa
     8821 gtgatgttcg accaatgttt cagccagatt gactatttgc gccgcaaagg gacgggcgcc
     8881 ggctccggtt cgtcggtcaa agtcgcccac ctgctcgaac ggctccggca gaccgtagac
     8941 cgtctaaagc tgctcaccga catccaaacc ggtgccggca acagcaaccg cctgaccatc
     9001 gcgctgatga actccctcat ctacgcggcg gtcgaacaat acagcacccg ccacctgcgc
     9061 cgcagcagca tccgtatgct cgcccgcagc attaccgaaa acaaaagcca ccacggcgaa
     9121 cactacatca cccgcaaccg caaagaatat ttcaaaatgt tctactcggc ggcaggcggc
     9181 ggcatcatca tcgccctgat ggcgctgctc aaaatccgca tcggcacact cggcctcagc
     9241 cccttcctga cttccttgtc ggctgggttc aactacggca tcggctttat gatcatccat
     9301 atgctgcact gcaccgtcgc caccaagcag cccgcgatga ctgccgccag ctttgccgaa
     9361 caggtcgatc tcaacgaagg cggcaaagcg gtggacaaca aactcgccaa gctcctcatc
     9421 gacgtatgcc gctcccaaag tgtcgccgtc ttcggcaacg tttccatcgc catccttttg
     9481 gcgtgcgcca tatcgttcgg ctatgcccat ctgtaccggc tgcccatact cgatgcccac
     9541 accgccgcct accagttcaa atccatagac atcatcgctt acccgacgct gtggtatgcc
     9601 gccattgcag gtctgtggct gttctgctcc ggcatcatcg caggtttttt cgacaaccgc
     9661 gccgactacc tcaacctgcg ccaacgcctg cccttcaacc ccttgctgcg taaaatcatg
     9721 cgccccgggc cccgccgcgt cctcgccgcc tacatccaca aacactacgg ctcgttggta
     9781 ggcaacttca tcttcgggat gctcttgggt atgaccggct atttcggaca cctcctcggg
     9841 ctgccgctgg acatccgcca cgtcgccttt tcctccgcca acctcggcta tgccgccgtc
     9901 agcggcaacg tcggtttggg cacgttcgta ctcggcattt tcagcgtcct caccatcggc
     9961 ctggtcaacc tctgcgtcag tttcagcctc gccctcttcg tcgccctgcg ctcgcgcggc
    10021 acgaaaatcg gcagcatccg caatctgatt aagagttttt ggaatcagat taaaagcaat
    10081 ccctgcatac ttttcctccc gcccgccaaa gaacagggac atcctccttc ggacaagcct
    10141 tgaccggcaa tgccgtctga agcgggcttc tccccgaata ccgccctgat gcggaaaatc
    10201 cccataaaag gatgcaaaaa tgccgtctga accgaaacgt ggttcagacg gcattttaaa
    10261 aggggctgta ctagattagc agatatgtta ccctcgaaat atgaagataa cgcactgcaa
    10321 attaaagaaa aaagtacaga aagaactgct cc
//
LOCUS       DAXHPG010000062        10228 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXHPG010000062 DAXHPG010000000
VERSION     DAXHPG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.062"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(121..1338)
                     /locus_tag="ACFM6F_001158"
     CDS             complement(121..1338)
                     /locus_tag="ACFM6F_001158"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC6164574.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1540..2268)
                     /gene="rph"
                     /locus_tag="ACFM6F_001159"
     CDS             complement(1540..2268)
                     /gene="rph"
                     /locus_tag="ACFM6F_001159"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC6164575.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2350..2814)
                     /locus_tag="ACFM6F_001160"
     CDS             complement(2350..2814)
                     /locus_tag="ACFM6F_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC6164576.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3031..3807
                     /locus_tag="ACFM6F_001161"
     CDS             3031..3807
                     /locus_tag="ACFM6F_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6164577.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4052..4906
                     /locus_tag="ACFM6F_001162"
     CDS             4052..4906
                     /locus_tag="ACFM6F_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC6164578.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNKHGIC
                     RFHDLFNPEQGAAYVVVNFISLLELAKEGLVRIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            4947..6155
                     /gene="pncB"
                     /locus_tag="ACFM6F_001163"
     CDS             4947..6155
                     /gene="pncB"
                     /locus_tag="ACFM6F_001163"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC6164579.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEVELDALCQLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6247..7965
                     /gene="argS"
                     /locus_tag="ACFM6F_001164"
     CDS             6247..7965
                     /gene="argS"
                     /locus_tag="ACFM6F_001164"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC6164580.1"
                     /translation="MNLHQTVEREAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIHAALNDA
                     RFGIAQTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVSEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGTDEAGKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDANAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8506..9924
                     /locus_tag="ACFM6F_001165"
     CDS             8506..9924
                     /locus_tag="ACFM6F_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6164581.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHSKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2356 a         3012 c         2569 g         2291 t
ORIGIN      
        1 atttaatcca ctatattttt tgttccaaag tcaaaatatg ccgtccgaac attcgggcgg
       61 cagacaaaac ggcactgccc gataaaggca gtgccgttgt ccgtttcaaa ccgtgaaaca
      121 tcagcccaaa ttaaaggctt tatgcaatac cctggttgcc agttccatgt atttttcatc
      181 aatcaatacg gaaactttga tttcggaggt ggaaatcatt tggatgttga taccctcttc
      241 ggcgagcgtg cggaagattt tggcggctac accgacgtgc gaacgcatac ccaaaccgac
      301 tgcggagact ttgcatacgg tgtcgtcgcc atcaatagaa gccgcgccga tactgtcttg
      361 gcgttccgac aggatttcca aagtctgctt gtaatcgccg cgcggtacgg taaaggaaaa
      421 atcggttgtg ccttcgctgc cgacattttg gataatcata tcgacttcaa tgttggcatc
      481 ggcaaccgcg cccaaaatct gataggcaac gccgggtttg tcgggtacgc cgcgcacgtt
      541 gatgcgggct tggtttttat cgaatgcgat accggttacg gcagctcttt ccatgttgtc
      601 gtcctcttca aaggtaatta aggtgccatt gccgccgtct tgcaggctgc tcagtacgcg
      661 caggcgcact ttgtactttc cggcgaattc tactgaacgg atttgcaaga ctttcgagcc
      721 gaggcttgcc aattcgatca tttcttcaaa tgtaaccgta tccatgcggc gcgcttcggg
      781 tacgacgcgc ggatcggtgg tgtacacgcc gtccacgtcg gtatagattt ggcattcgtc
      841 cgctttgagg gctgcggcaa gcgcaacggc ggaagtgtcg gaaccgccgc gtccgagcgt
      901 ggaaatatcg ccttcactgc tgatgccttg gaagccggca acgatgacga ctttgccggc
      961 ggtaaggtcg gcacgcattt tttcgtcatc aatgctttcg atgcgggctt tggtgtgggc
     1021 ggtatcggtt ttgagggcga cctgccagcc tgtgtagctt ttggcatcca cgccgatgtc
     1081 tttcaatgcc atcgccaaaa ggccgatggt tacttgttcg ccggtggaca aaaccacgtc
     1141 caattcgcgc ggatcgggat gctcctgcat ttcgtgcgcc aacgcgacca ggcggttggt
     1201 ttcaccgctc atggcggata cgacgacgac gatgtcgtgt ccttcggcgc gggctttggc
     1261 gacacgtttg gccacgtttt taatgcgttc gggcgagcct acggatgtgc cgccgtattt
     1321 gtgtacgatt aacgccatgt ttcgtgcttt cttgtgtggg attggtcggg cagcatggtt
     1381 tgctggaaaa gcggctatta ttactatttt ttacatgaag ttcaagaacg gactgcgctt
     1441 tcccgcctgc cgtttgacag cggtcagcga aaaacctgtt ctttcagatt gttgacaaaa
     1501 tgccgtctga acggttttca gacggcatcc ggacgacaat caggcggcgg acaacgcatt
     1561 ttgctggtgt tgcagcagtt cgcctatgcc tttttgcgcc agtgcaacca gtttgcccaa
     1621 ttcgtccaaa ctgaacggcg cgtcttccgc cgtaccttgg atttcgatga tttttcccga
     1681 tgcggtcatg acgatattca catcactgtc gcaaccggag tcttcgggat aatccaaatc
     1741 caaaagcggc acgccgttca ctacgcctac tgacacagcg gcaacggctt cgcggatggg
     1801 gttttcactc aaaatgccgt ctgaaaccag tttgccgacg gcgatttgca gcgcgacaaa
     1861 cgcaccggta atcgaagccg tacgcgtacc gccgtctgcc tgaatcacgt cgcagtcaat
     1921 caagatttgg cgttcgccga gtttttccat atccacgacc gcgcgcagcg aacgcccgat
     1981 aaggcgttgg atttcttgtg tgcgcccgga ctgtttgccc gccgaagctt cgcggcgcat
     2041 ccgggaagca gttgaggcag gcagcatccc gtattccgcc gttacccagc cttggttttt
     2101 accgcgcaga aacggcggga cgttttcatc tatggaagcg gtacaaatca ctttggtatt
     2161 gccgcattca ataaggcacg aaccgtccgt atgcggcagg aaatgagggg tgattttgat
     2221 atcgcgcagg ctgtcggcgg cgcgcgagat gcggatgtaa tcaggcatac tgccctcccg
     2281 ttaaaaacag ataaattaaa aagccttaaa tatgaaaaat cacatttaag gccttcaaac
     2341 tgaaaatttc tacgcctctt cggctttgct gcggataatc aaaagcggca ggtggctttg
     2401 gcgcattacc gtttcggcaa aactacccat taaaaggtgc atcagtccgg tacgtccgtg
     2461 cgtacccaac accagcaggt cggcaccgtt ttcatcggca taatcaacca aatcctgcgc
     2521 catttcacgc gcacccttat tggcaaccag caggtgtttg acggtatttt ccacacccag
     2581 ttcccgggcg gtgcgctcgg cggcatccaa aacttcgttg ccttgcgcga cggcggcggc
     2641 ttcgtagctt tcgtgttgca aaaattcggg ggcgagcgcc atatattcgg caggattggc
     2701 aacgtgtacc aaagtcagac gcgcaccgtt gaccccggca agctcggcgg catgtttcag
     2761 ggcattgatg gacgtttcac tgccgtcaac ggcaacaacc aaatgtttgt acatatcgta
     2821 ttctcctttt gcaccgcctc gcggtgccct cttgtcggat gggcgcaggg acagtttgcg
     2881 ctgtttcatt atagacccgc cgtcgggctt tatacaacag ccgaacagcc cgaccgcttt
     2941 ccagtataat atgccgcttc cgtgcagtca ggcatttttt gccggctttc gttcactttt
     3001 tgatttgacg caatcttgca ggattcgacc atgtccgaca acgctttgac ctcttcgcga
     3061 cgcttcggcg gcatcgccag actctacgga gactctgcct tggcgcactt ttcacaggca
     3121 cacgtctgcg tagtcggcgt gggcggtgtc ggctcgtggg cggtcgaggc tttggcgcgg
     3181 acgggcatcg gacgtttgac tttgattgat ttggacaacg ttgccgaatc gaatgtcaac
     3241 cgccagctgc acgccctgac cggagacttc ggcaaagcaa aagttaccgc cttgcgcgaa
     3301 cgcgtcgccc aaatcaaccc agaatgccaa gtgtttgaaa ttgaagattt cgttaccgaa
     3361 gacaatttgc cggaatactt cggaaaaggt tttgatttcg tcatcgacgc gatcgaccaa
     3421 gtgcgcgtca aagcagcaat ggcggcttat tttgtggaac gcaaacaacc gtttgtcctc
     3481 agcggcggcg cgggcggaca aaaaaatccg gcgttaatcc aaaccgccga tttgagccgc
     3541 gtaacccacg acccgctgct tgccaacctg cgctacacct tgcggaaacg ctacggattc
     3601 agccgcgata cgaaagcaaa tatgcgcgtg ccttgcgtgt attcgaccga aaatatcgtg
     3661 ccgccgcagt ctagggaggc ttgttcggca gatgccgctc cgcaaggctt gtcgtgcgcc
     3721 ggctacggtg caagcatgct cgttaccgct tcgttcgggc tatattgcgc acaggcggcg
     3781 gtggaacaca tcgcagacaa aaaataagca atgccgtctg aaacaggatt cagacggcat
     3841 ttgaacaaac gcggcaatca agctgtcggt tcagaaaaac caacatcggc acgcctaaaa
     3901 ccgtattcac aacctgctcc ttttcaaaac atttgcattt aaaagccgtt ataatgccgt
     3961 ctgaacattt gcccgaccac attatgcgtg aatgtcggca gattgttttc ttttgtaaac
     4021 ttatattaaa atccacttac cgattcacgc catgccgccc atccctgccc catccgcacc
     4081 atccgagcac actgtcgcat gggtattcgg ccaacccgtt accgatttgc cgcaggattt
     4141 gtttatcccg cccgacgcgc tgaaagtcgt attgggcagt tttcaaggcc ctttggatct
     4201 actgctgtat ctgatccgca agcagaacat cgatgttctc gatattccga tggtgaagat
     4261 taccgagcag tatctgcact acatcgccca aatagaaacc tatcagtttg atttggcggc
     4321 ggaatatctt ttgatggcag caatgctgat tgaaatcaaa tcgcgcctgc tgctgccgcg
     4381 taccgaaacc gtcgaagacg aagaagccga cccgcgtgcc gagttggtgc gccgcctgct
     4441 ggcttacgaa cagatgaagc tggcggcgca gggtttggac gcgctgcccc gagccggacg
     4501 ggatttcgcg tgggcttacc tgccgctgga aattgccgtc gaagccaagc tgcccgaagt
     4561 ctatattacc gacttgacgc aagcgtggct gggtattttg tctcgggcaa aacatacgcg
     4621 cagccacgaa gttatcaaag aaaccatctc cgtgcgcgcg caaatgacgg caatcctgcg
     4681 ccgtttgaac aaacacggga tatgcaggtt tcacgacctg ttcaatcccg aacagggcgc
     4741 ggcttacgtg gtcgtcaact tcatctcact gttggagctt gccaaagaag gattggtcag
     4801 aatcgtgcag gaagtcggtt tcggagaaat tcgaatcagc ctcaatcatg agggggcgca
     4861 ttcagacggc atttccggca cacggggcgg gcgcgatgtg ttctaatacg ccccaagccg
     4921 ccaccaaaaa tcgggagaca cgccatatga ccggcatcat acattcgctg cttgacaccg
     4981 acctctacaa attcactatg ctgcaagtgg ttctgcacca gtttccgcag acgcacagcc
     5041 tttacgaatt ccgctgccgc aacgcctcga ccgtctatcc gcttgccgac atcaaagaag
     5101 acttggaagt cgaactcgac gcgctctgcc aactgcgctt tacccatgac gaactcggct
     5161 acctccgcag cctgcgtttc atcaaaagcg actttgtcga ttatctcgaa ctcttccagc
     5221 tccaacgccg ctttgtcgaa atcggcacag acgataaaga ccgtctgaac atccgcatcg
     5281 aaggtccgat gatacaggcg atgttctttg aaatcttcat cctcgccatt gtcaacgaac
     5341 tttacttccg ccgcctggaa acccctgcag tcatagaaga aggcgaacgc cgccttcaag
     5401 ccaaagccgc gcgcctcaaa gaaatcgccg ccgcacaaaa ccccgacgaa ccgcccttcc
     5461 tgatttccga cttcggcacg cgccgccgct acaagctcgc gtggcaggaa cacgtcatcc
     5521 gcaccctgct cgaagccgcc ccaagcatcg tgcgcggcac cagcaatgtc tttctcgcca
     5581 aaaaactcgg catcaccccc atcggcacca tggcgcacga attcctccaa gccttccagg
     5641 ccctcgacgt acgcctgcgg aatttccaaa aggccgcgct cgaaagctgg gtgcacgaat
     5701 accggggcga tttgggcgtt gccctgaccg acgtggtcgg tatggatgcc ttcctgcgcg
     5761 atttcgacct ctatttcgcc aaacttttcg acgggctgcg ccacgacagc ggcgaccctt
     5821 acgtttgggg cgacaaagcc tacgcccact atcaaaagct caaaatcgac agccgcacca
     5881 aaatgctgac cttctccgac gggctggaca tcgaacgctc ttgggcatta caccaatatt
     5941 tcaaagaccg cttcaaaacc ggcttcggca tcggcaccaa cctcaccaac gatatggggc
     6001 atacgccttt gaatatcgtg ttgaaactgg tcgaatgcaa cgggcagtcc gtcgccaagc
     6061 tgtccgactc tccgggcaaa accatgacca acaacagcac cttcctcgcc tacctgcgcc
     6121 aagtgttcga cgtacccgaa cccgaaacgc cgtaaaccgg cagaaaaagc gcacaattcc
     6181 tgtttctgcc gcataaaatc ttttaaaata ccgcctgatt tgaatttaac cgaaagaccg
     6241 aacttcatga acctacatca aaccgtcgaa cgcgaagccg ccgccgcctt tgccgccgca
     6301 ggcatcgccg gcagccccgt tgtcttgcag ccgaccaaaa atgccgaaca cggcgatttc
     6361 caaatcaacg gcgtgatggg tgcggcgaaa aaagccaaac aaaacccgcg cgaactggcg
     6421 caaaaggtcg ccgacgcatt ggcgggcaac gccgtcatcg aaagcgcgga agtcgccgga
     6481 cccggcttta tcaacctgcg cctgcgtcct gaatttctcg ctcaaaacat tcatgcggct
     6541 ttgaacgatg cgcgtttcgg catagcgcaa accgacaaac cgcaaaccgt cgttatcgac
     6601 tattcctcgc ccaatctggc aaaagaaatg cacgtcggcc atctgcgttc cagcatcatc
     6661 ggcgacagca tttcgcgcgt gtcagaattt atgggcaata ccgttatccg tcaaaaccac
     6721 gtcggcgact ggggtacgca gtttggcatg ttggtcgctt atttggtcga gcagcaaaaa
     6781 gacaatgccg cattcgagct ggcggatttg gagcagtttt accgcgccgc caaagtgcgc
     6841 tttgacgaag accccgcctt tgccgacacc gcgcgcgaat acgttgtgaa gctgcaaggc
     6901 ggcgatgaaa ccgttttggc attgtggaaa cagtttgtcg atatttcgct ctcgcacgcc
     6961 caagccgttt acgacacgct gggcttgaag ctgcgccccg aagacgtggc gggcgaatcg
     7021 aaatacaacg acgatttgca gcccgtagtc gatgatttgg ttcaaaaagg tctggcagtc
     7081 gaagacgacg gcgcaaaagt cgtgttcttg gacgagttta aaaacaaaga gggcgaacct
     7141 gccgcattta tcgtgcaaaa acaaggcggc ggcttccttt atgcttccac cgacttggcg
     7201 tgtttgcgct accgcgtcgg cacgcttcat gccgaccgcc tgctgtacgt cgtcgaccac
     7261 cgccaagccc tgcacttcga acaacttttc accacttcac gcaaagccgg ctatctgccg
     7321 gaaaacgtcg gcgcggcatt tgtcggcttc ggcacgatga tgggcaaaga cggcaaaccg
     7381 ttcaaaacgc gcagcggcga caccgtaaaa ctggtcgatc tgctgaccga agccgtcgag
     7441 cgcgccaccg ctttggtgaa agaaaaaaat cccgaattgg gtacagacga agcaggaaaa
     7501 atcggtaaaa ccgtcggcat cggcgcagtc aaatacgccg acttgagcaa aaatcgcacc
     7561 agcgattatg tgttcgactg ggacgccatg ctctcgtttg aaggcaacac cgccccctat
     7621 ctgcaatacg cctacacccg cgtgcaaagc gtgttccgca aagcaggcga atgggatgca
     7681 aatgcgccaa ccgttttgac cgaaccgctg gaaaaacagc ttgccgccga gctgctgaaa
     7741 tttgaagacg tactgcaaag cgtggcggac acggcgtatc cgcactacct cgccgcctac
     7801 ctctatcaaa ttgcgaccct gttcagccgc ttctacgaag cctgtccgat actcaaagcc
     7861 gaaggcgcaa gccgcaacag ccgcctgcaa ctggcaaaac tcaccggcga cacgctgaaa
     7921 caaggcttgg atttgctggg catcgatgtg ttggacgtaa tgtaaaaccg caccgcccga
     7981 ttgcggacaa cagcctcgcc atccttatcc gaatctgaaa aaagcggcgc gatacaccgt
     8041 atccgccgcc cctcccaaaa tgcgaaacaa acaaacgcca agcaagcaag caagcaagca
     8101 agcaagcaag caaaaaatta taaccccctt cctgccgacg cacgcacttt ccgcgcggcg
     8161 cattcccctt ttcccgcccc tcaaatccgc cttttcttca ggcagggttt cagcccgcct
     8221 cttttccctg ttttccttcc cccgacacgc gtgcgcgccc cctgccacac cgtgccgcac
     8281 tttcgcgccc ggacggcatc gttccgccat ccggttctct gttttaaata cccctgtttc
     8341 agaaagaaat tgcagatgtt tcaacacaca ggacgacaca taaagcaccg ccctatgtgt
     8401 tgccctgatt tggaaggggt tacacccttc ccaaatatag tctgatcctg ccgccccgaa
     8461 ggacagatgt ccgagtggcg aagtttcaac cgaaaaggaa atacgatgaa tattcacgcc
     8521 ctgctctcca aacaatggac gctgccgcca ttcctgccga aacggctgct gctgtccctg
     8581 ctgatactgc ttgcccccaa tgcggtgttt tgggttttgg cactgctgac cgccaccgcc
     8641 cgcccgattg tcaatttgga ctatcttccc gccgcgctgc tgatcgccct gccttggcgt
     8701 ttcgtcaaaa ttgccggcgt attggcgttt tggctggcgg ttttgtttga cgggctgatg
     8761 atggtgatcc aactcttccc ttttatggat ctcatcggcg ccatcaacct cgtccccttc
     8821 atcctgaccg cccccgcccc ttatcagata atgaccgggc tgttgctgct gtatatgctg
     8881 gcgatgccgt ttgtgttgca gaaagccgcc gccaaaaccg acttccggca cattgccgtc
     8941 tgcgccgccg ttgtggcggc agccggctat ttcaccggcc atttgagtta ctacgaccga
     9001 ggtcggatgg ccaatatctt cggcgcaaac aacttctact acgccaaaag tcaggcgatg
     9061 ctctacaccg tcagccagaa tgccgacttt attaccgccg gcctggtcga tcccgtcttc
     9121 ctccccttgg gcaatcaaca gcgtgccgcc acgcatctga acgagccgaa atctcaaaaa
     9181 atcctcttta tcgtcgccga atcttggggg ctgccggcca atcccgaact tcaaaacgcc
     9241 acttttgcca aactgctggc gcaaaaagac cgtttttcgg tttgggaaag cggcagtttt
     9301 ccctttatcg gcgcgacggt cgaaggcgaa atgcgcgaac tgtgcgccta cggcggtttg
     9361 cgcgggttcg cactgcgccg cgcgcccgac gaaaaatttg cccgctgcct ccccaaccgt
     9421 ttgaaacaag aaggttacgc cacctttgcg atgcacggcg cgggcagttc gctttatgac
     9481 cgcttcagct ggtatccgag ggcgggcttt caagaaatca aaaccgccga aaacctgatc
     9541 ggtaaaaaaa cctgcgccat tttcggcggc gtgtgcgaca gcgagctgtt cggcgaagtg
     9601 tcggcatttt tcaaaaaaca cagcaaagga ttgttttact ggatgacgct gaccagccac
     9661 gccgactatc ccgaatccga cattttcaac cacaggctca aatgcaccga atacggcctg
     9721 cccgccgaaa ccgacctctg ccgcaacttc agcctgcaca cccaattctt cgaccaactg
     9781 gcggatttga tccaacgccc cgaaatgaaa ggcacggaag tcatcatcgt cggcgaccat
     9841 ccgccgcccg tcggcaacct caatgaaacc ttccgctacc tcaaacaggg gcacgtcgcc
     9901 tggctgcact tcaaaatcaa ataacaacaa tgccgtctga acgcaccaac agccttcaga
     9961 cggcattttg cagacagacc gacccttcaa gcccactttt ttcatcatct ccgataaatt
    10021 gctttgtata gtggattaac aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga
    10081 acgattctct aaggtactga agcaccaagt gaatcggttc cgtactattt gtactgtctg
    10141 cggcttcgtc gccttgtcct gatttttgtt aatccgccat aaagaccgtc gggcatctgc
    10201 agccgtcatt cccgcgcagg cgggaatc
//
LOCUS       DAXHPG010000063         9811 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXHPG010000063 DAXHPG010000000
VERSION     DAXHPG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.063"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..457
                     /locus_tag="ACFM6F_001166"
     CDS             <1..457
                     /locus_tag="ACFM6F_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HFC6164582.1"
                     /translation="YVMNNFAGTGLEEALIQFISARLGDLKSKYDVHLIRNEEVFKLN
                     NFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFIEPKGEHLVETDGWKEEFLEAI
                     TVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTESFPLGAASLEKMK"
     gene            complement(601..1773)
                     /locus_tag="ACFM6F_001167"
     CDS             complement(601..1773)
                     /locus_tag="ACFM6F_001167"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC6164583.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            2123..2569
                     /gene="iscR"
                     /locus_tag="ACFM6F_001168"
     CDS             2123..2569
                     /gene="iscR"
                     /locus_tag="ACFM6F_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC6164584.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNSDGSRVVQFTHIH"
     gene            2598..3812
                     /locus_tag="ACFM6F_001169"
     CDS             2598..3812
                     /locus_tag="ACFM6F_001169"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC6164585.1"
                     /translation="MTVKTPVYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILVSVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLGHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            3958..4803
                     /locus_tag="ACFM6F_001170"
     CDS             3958..4803
                     /locus_tag="ACFM6F_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFC6164586.1"
                     /translation="MKIANALIHNTVRIECLSADGQSISTGTAFLFLFDFDKTGVIPV
                     LVTNKHVVFVESAKKIAITLTKDENGSPNHKENITFTIEDFIQNCLPHPDENIDLCIA
                     PVGHFFNQLINHNFSPFIKGIRESDIMQTEEMDQLSAFEEVFMIGYPNGLWDSKNNLP
                     IFRTGITATHPAIDFEGKKEFMIDVACFPGSSGSPVFKYIPAGLRKIENDGFAITADE
                     TFKLMGILYAGPQQTISGEIYITTVNKPISVSSIPINLGFVIKAEKLLDFKPILKNFL
                     EKNRE"
     gene            4950..5078
                     /locus_tag="ACFM6F_001171"
     CDS             4950..5078
                     /locus_tag="ACFM6F_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164587.1"
                     /translation="MNEQELDLDNLDNLLDDFDGVTVEGGVDSENDEGCEGGACKI"
     gene            5146..5532
                     /gene="iscU"
                     /locus_tag="ACFM6F_001172"
     CDS             5146..5532
                     /gene="iscU"
                     /locus_tag="ACFM6F_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC6164588.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            5619..5939
                     /gene="iscA"
                     /locus_tag="ACFM6F_001173"
     CDS             5619..5939
                     /gene="iscA"
                     /locus_tag="ACFM6F_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC6164589.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(5899..6120)
                     /locus_tag="ACFM6F_001174"
     CDS             complement(5899..6120)
                     /locus_tag="ACFM6F_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC6164590.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            6202..6702
                     /gene="hscB"
                     /locus_tag="ACFM6F_001175"
     CDS             6202..6702
                     /gene="hscB"
                     /locus_tag="ACFM6F_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC6164591.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            6800..9550
                     /gene="gyrA"
                     /locus_tag="ACFM6F_001176"
     CDS             6800..9550
                     /gene="gyrA"
                     /locus_tag="ACFM6F_001176"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC6164592.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIRLIKESPNAVEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            9707..9811
                     /locus_tag="ACFM6F_001177"
                     /pseudo
     CDS             9707..9811
                     /locus_tag="ACFM6F_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         2807 a         2655 c         2191 g         2158 t
ORIGIN      
        1 gtatgtgatg aataattttg ccggaacggg tttggaagaa gcgttaattc aatttatttc
       61 agcgcgtttg ggcgatttga aatctaaata tgacgttcat ttaatccgta atgaagaagt
      121 gtttaaattg aataactttg ccgatggtga aggatttatg ccggacttta ttttattgct
      181 gaaagataaa caaaaatctt cttccaatgg tgtgaacgac tttttgcatt accaaatttt
      241 cattgagccc aaaggtgagc atttagtgga aactgatgga tggaaagaag aatttttaga
      301 agccatcact gtggaatatg ggatggataa gatcctgcaa aaagatacac cgcattatcg
      361 tttgatcggt ttaccgtttt ttactgatca tcagaaaaat ggacaattta cggagtcatt
      421 ccctttaggt gcggcatcgc ttgaaaaaat gaagtagtgc attgcaggca accccgtttg
      481 acaaaacttc ctttacaaaa gggcgttttg tcagatattt aatcaacaca ttattaaaat
      541 acagccaaat tttaatgccg tccgaaccct gtgttcagac ggcatcgtat ttttcagtat
      601 ctaaaccgtt tccctgcccc aatctttgcc tctcaaaatc gaagcatcga catcttgaat
      661 atcgcggtgt cccgtaaacg ccatagatat atccatttct ttatacagga tttccagcgc
      721 acgggttacg ccttcttcac catacgcacc caaaccatac agaaacgccc ggcctatcat
      781 cgtacctttc gcgcccaaag cccacgcctt caaaatatcc tgaccgctgc ggataccgct
      841 gtccatccaa acttcgatgt cgctgcccac tgcgctgacg atgtcgggca aggctttgat
      901 ggcagacacg gtatcgtcga gctgtcggcc gccgtggttg gaaacaatca atgcgtccgc
      961 gccgcttttc gctgcttttt ccgcgtcttc gggttccata atgcctttga taatcagctt
     1021 gccgccccac aaatctttaa tacgcgccac atcgtcccag ctcaggcgcg ggtcgaattg
     1081 ttcggaagtc catgaagaca gcgaagacaa atcgccgacg ttcttcgcgt gtccgacgat
     1141 attgcggaac gtgcggcgtt ccgtgttcag cattttcatg caccattccg gtttggtcgc
     1201 cagattgatt aaattggcga tggtcggttt cggcggcgcg gacaggccgt ttttgatgtc
     1261 tttgtggcgt tgccccaaaa cctgcaaatc ggcggtcaaa accaatgccg aacatttggc
     1321 atccttcgcg cgcttaatca ggttttccat aaattcgcgg tcgcgcatca cataaagctg
     1381 aaaccaaaac ggcgcgctgg tgttctcggc aacgtcttca atcgagcaga tggacatggt
     1441 ggacaacgta aacggaatac cgaacttctc cgccgcccgc gccgccaaaa tttcgccgtc
     1501 tgcgtgtgcc atgccggtaa aacccgtcgg cgcaatcgcc accggcattt tcacatcctg
     1561 cccgatcatt ttagtctcaa ggctgcgtcc ctccatattg accaatactt tttgacggaa
     1621 gcggatgtct ttgaaatccg aagtattttc ccgataagtc gtttccgtcc acgaacccga
     1681 atcgatgtaa tcgtaaaaca tacgcggcat tttgcgcttg gcaacgcggc gcaagtcttc
     1741 gatgcaggtc attttgctca aatcacgttt catcttgttt ttccctgttt gacagcctga
     1801 tggaatatgc cgtccgaaac cgcttgagcc ttcaaacggc atttcaggca atacccaaat
     1861 aaatcactaa gatttagata atctatatca gtatcaaaaa taaatcaaat cgttttgaaa
     1921 ccgcttgccg gcaaaatacc ccgaccgccg ctcctcaaaa aacataacca actgatttaa
     1981 aaaaccaaac aaataaagta aaatcagact ataccgccat ttaaacaaac aatcggcaac
     2041 agctccccct tacttgactg aaacactcag atattggaca attctcccca ctataaaaaa
     2101 gccgacacgg gcaaccacca ccatgagact gaccaccaaa gggcgttttg ccgttaccgc
     2161 tatgctggat ttggcgatga acgcgcaaac cggcgccgtc aaactcagtg ccatcagcga
     2221 acgccaaaac atatccctct cctatctcga gcaattgttc ggcaaactcc gccgcgccgg
     2281 actggttgaa agcctgcgcg ggcccggcgg cggctacatc ctcgccgcac cggcggcacg
     2341 catcaacatc gcccaaatca tcgccgccgc cgaagaccgg ctggacgcga cccaatgcgg
     2401 cagcaaagcc aactgccacc acggtgcgcc ctgcctgacg cacgaccttt gggagaattt
     2461 aaacaaaacc atcaacgact acctcggcag cgttaccctg caaagcatca tcgaacagaa
     2521 aaacaacagc gacggcagcc gcgtcgtcca atttacacac atccattaaa taacacccga
     2581 aaaagaaaga gcaaaccatg accgtcaaaa cccccgttta cctcgactac gccgccacca
     2641 ccaccgttga caaacgcgtt gccgaaaaaa tgattcccta tctgaccgaa accttcggca
     2701 accccgcctc caacagccac gcattcggct gggaagcaga agaggccgtc gaaaaagccc
     2761 gcgccgacat cgctgccctg attaatgccg accccaaaga aatcgtcttc accagcggcg
     2821 cgaccgagtc cgacaacctc gccatcaaag gcgcggcaaa cttctacaaa accaaaggca
     2881 aacacctcat taccgtcaaa accgaacata aagccgtttt agatacaatg cgcgaactcg
     2941 aacgccaagg ttttgaagta acctacctcg gcgttcaaga aaacggtttg attgatttgg
     3001 aagaactcaa agccgccatc cgcgacgaca ccatcctcgt ttccgtaatg tgggcaaaca
     3061 acgaaatcgg cgtggtgcaa gacattcccg ccatcggcga aatctgccgc gaacgcaaaa
     3121 tcgtcttcca cgtcgatgcc gcccaagcct gcggcaaagt gcctgtcgat gtcgaagccg
     3181 ccaaaatcga cctgctctcg atgtccgcgc acaaagtgta cggccccaaa ggcatcggcg
     3241 cgctgtacgt ccgccgcaaa ccccgcgtcc gcctcgaagc ccaaatgcac ggcggcggcc
     3301 acgaacgcgg cttccgcagc ggcacattgc cgacccacca aatcgtcggt atgggcgaag
     3361 ccttccgcat tgccaaagaa gaattggcac aagacactgc gcactacctg aaactgcgcg
     3421 atatcttcct caaaggcatc gaaggcatcg aagaagtcta catcaacggc gacctcggac
     3481 accgcgcccc gaacaacctg aacgtcagct tcaacttcgt cgaaggcgaa agcctgatta
     3541 tggcggtgaa agaactcgcc gtatccagcg gctccgcctg cacctccgcc tcgctcgaac
     3601 ccagctacgt cctgcgcgcg ctcggccgca acgacgaact ggcgcactca tccctgcgta
     3661 tcaccttcgg tcgcatgacc accgaagaag aagtgcaatt cgccgccgaa ctgattaaat
     3721 ccaaaatcgg caaactgcgc gaactgtcgc cgctgtggga aatgttcaaa gacgggattg
     3781 atttgaactc gattgaatgg gcggcgcatt aatttatgaa ttagtacgat gatatgagta
     3841 tagtagataa gattttcaaa tattctcgcc acctcgatta ttgagatata gtgaacttct
     3901 tatctacgga taaaattttt atagatagtt taatatttaa atagaactta aaaaattatg
     3961 aaaatagcca atgcattaat tcacaataca gtccgtattg aatgcctttc agcagacggc
     4021 caatctatat caacgggaac ggcattctta ttccttttcg atttcgacaa aacaggggta
     4081 attccagttc ttgtaacaaa taaacatgtt gtttttgtag agtccgcaaa aaaaatagct
     4141 attactttga caaaagatga aaatggctct ccaaatcata aagaaaatat aacttttaca
     4201 attgaagatt ttatacaaaa ttgtttacct catcctgatg aaaatattga tctatgcatt
     4261 gctcctgttg gacatttttt taatcagctg attaatcata atttctcgcc ttttataaaa
     4321 ggaataagag aatctgatat tatgcaaact gaagaaatgg atcaattatc agctttcgaa
     4381 gaagttttta tgataggcta tcctaatggc ttatgggact caaaaaacaa cttaccaata
     4441 tttagaacag gaattactgc tacccatcca gctattgatt ttgaagggaa aaaagaattt
     4501 atgattgatg ttgcctgttt ccctggttca agcggttctc cggtgttcaa atatatacct
     4561 gctgggttaa gaaaaataga aaacgatggg tttgcaatta cagcagatga aacatttaaa
     4621 ttaatgggaa tcctatatgc tggacctcag caaacaatat caggggaaat ttatataaca
     4681 actgttaata aaccaatttc tgtatcttct attcctatta atttaggttt tgtcataaag
     4741 gcagaaaaac ttttagactt taaaccaatt ttaaagaatt ttttggaaaa aaatagagaa
     4801 tgaaattata ttaaaagatt aataaatatt agagactggc aatccacctt acctaatcta
     4861 cattaaaaat aaaaaagacc gtttgaaaca tcaaaaagat tttttcaaac gacctaaaag
     4921 ccaccacatg aaatcaagga tacaaaacca tgaacgaaca agaacttgac ctcgacaatc
     4981 tcgacaacct gcttgacgat tttgacggcg ttaccgtcga aggcggcgtc gattcggaaa
     5041 acgacgaagg ctgcgaaggc ggcgcgtgca aaatctgact caggccgtct gaaaaccggc
     5101 aaacaaacca ctttaaacta tcaaaaacat taaggaaacc acatcatggc atacagcgat
     5161 aaagtaatcg accactacga aaatccccgc aacgtcggta ctttcgacaa aaacgacgaa
     5221 tctgtcggca ccggcatggt cggcgcgccc gcctgcggcg acgtcatgcg cctgcaaatc
     5281 aaagtgaacg acgagggcat catcgaagat gcgaaattta aaacttacgg ctgcggctcg
     5341 gccatcgctt cgtccagcct gattaccgag tgggttaaag gcaaaagcct ggatgacgcg
     5401 ctggcaatca aaaacagcga aatcgccgag gagttggaat tgccgccggt aaaaatccac
     5461 tgctccatct tggctgaaga tgcggtaaaa gcggccgttg ccgactaccg caaacgtcag
     5521 gaaaacagat aaagcccttc agacggcatc atcccgcaat gccgtccgaa ccacccgccg
     5581 cttcaggtcc gtccggggcg gtacaacaag gaagaaatat gattaccctt accgagaatg
     5641 ccgcaaaaca catcaatgac tatctcgcca aacgcggcaa aggcttgggc gtacgcttgg
     5701 gtgtgaaaac cagcggctgc tcggggatgg cgtacaacct tgaatttgtc gacgaagccg
     5761 atggcgacga cctgattttc gaaggacacg gcgcgcgcat ttatatcgat ccgaaaagcc
     5821 tggtttatct ggatggcacg caagtcgatt acaccaaaga aggtttgcag gaaggtttca
     5881 aatttgaaaa ccccaatgtc aaagactcct gcggctgcgg cgaaagcttc cacgtttaag
     5941 gcataaaaac ggcgggaccg tatcaaaacc gtcccgccat tttttcgctt cctgcctgtt
     6001 gtagctgcct ttgcctttcc ttttccgttc caccttgtgc cggaacaaat cggatttcac
     6061 taaggctttt aaagcattgt cgcgtatttt gcctttattg tgctgcactt tgccgcccat
     6121 attcagtcct ttcgtttaag aagcggcaga ttataaggca aaaacagttt tctgccaaaa
     6181 tcttacattt atcatcctac tatgtcccaa tatttcaccc tcttccggat tgaacccgct
     6241 ttcgatatcg acaccgaaaa cttggaacaa acctaccgcg ccttggccgc ccgtttccat
     6301 cccgataaat tcgcttcagc ttccgccttt gagcaaaagc aggcagtgat gatgtcttcc
     6361 accatcaacg atgcctaccg caccttgaaa aaccccatcg accgcgccgc ctacctgctg
     6421 aaaacatcgg gcatcgatgc cgacgcgccg gagcataccg ctttcgcccc cgaattcctt
     6481 atgcagcaaa tggaatggcg cgaaacgctg atggaggcac gggcaggcaa cgaccttgaa
     6541 tccttgaaaa atctcgacaa cgaaatccgc gacgagcaag aaaaactgtt ctgcggtctg
     6601 aaacagtcgt ttgcccgaca agattacgac acagccgcac aacaagtccg ccaaggcagg
     6661 tttctcgaca aactccgcaa cgaaatttcc tcggcattat aatccgcacc gtgtttcaga
     6721 cggcgtaacc gccgcaccgt tccgcgtcaa aatatgctaa aataagcaac aattttttgc
     6781 catacgaaac attgaaacca tgaccgacgc aaccatccgc cacgaccaca aattcgccct
     6841 cgaaaccctg ccggtaagcc ttgaagacga aatgcgcaaa agctatctcg actacgccat
     6901 gagcgtcatt gtcgggcgcg cgctgccgga cgttcgcgac ggtctcaagc cggtacaccg
     6961 ccgcgtactg tacgcgatgc acgaattgaa aaacaactgg aatgccgcct acaaaaaatc
     7021 ggcgcgcatt gtcggcgacg tcatcggtaa ataccacccc cacggcgata ccgccgtata
     7081 cgacaccatc gtccgtatgg cgcaaaattt cgctatgcgt tatgtgctga tagacggaca
     7141 gggcaacttc ggatcggtgg acgggcttgc cgccgcagcc atgcgctaca ccgaaatccg
     7201 catggcgaaa atttcccacg aaatgctggc agacattgag gaagaaaccg tcaatttcgg
     7261 cccgaactac gacggtagcg aacacgagcc gcttgtactg ccgacccgtt tccccacact
     7321 gctcgtcaac ggctcgtccg gcatcgccgt cggcatggcg accaatatcc cgccgcacaa
     7381 cctttccgat accgtcaatg cctgcctgcg cctgctcgat gcacccgaca ccgaaatcga
     7441 cgaactgatc gacattatcc aagcccccga cttcccgacc ggggcaacca tctacggctt
     7501 gagcggcgtg cgcgaaggct ataaaacagg ccgcggccgc gtcgttatgc gcggtaagac
     7561 ccatatcgaa cccataggca gaaacggcga acgcgaagcc atcgttatcg acgaaatccc
     7621 ctatcaggtc aacaaagcca agctggtcga gaaaatcggc gatttggttc gggaaaaaac
     7681 actggaaggc atttccgagc tccgcgacga atccgacaaa tccggtatgc gcgtcgttat
     7741 cgagctgaaa cgcaacgaaa atgccgaagt cgtcttaaac caactctaca aactgactcc
     7801 gctgcaagac agtttcggca tcaatatggt ggttttggtc gacggacaac cgcgcctgtt
     7861 gaacctgaaa cagattctct ccgaattcct gcgccaccgc cgcgaagtcg ttacccgacg
     7921 tacgcttttc cggctgaaga aggcacgcca tgaagggcat atcgccgaag gcaaagcggt
     7981 cgcactgtcc aatatcgatg aaatcatccg gcttatcaaa gaatcgccca acgcggtcga
     8041 ggccaaagac aaactgcttg cgcacccttg gcgcagtagc ctcgttgaag aaatgctgac
     8101 gcgttccggt ctggatttgg aaatgatgcg tccggaagga ttggctgcaa acatcggctt
     8161 gaaagagcaa ggttattacc tgagcgagat tcaggcagat gctattttac gcatgagcct
     8221 gcgaaacctg accggcctcg atcaagaaga aattgtcgaa agctacaaaa acctgatggg
     8281 taaaatcatc gactttgtgg atatcctctc caaacccgaa cgcattaccc aaatcatccg
     8341 cgacgaactg gaagaaatca aaaccaacta tggcgacgaa cgccgcagcg aaatcaaccc
     8401 gttcggtggc gacattgccg atgaagacct gattccgcaa cgcgaaatgg tcgttaccct
     8461 gacacatggc ggctatatca aaacccagcc gaccaccgac tatcaggcgc agcgtcgcgg
     8521 cgggcgcggc aaacaggcgg ctgccaccaa agacgaagac tttatcgaaa ccctgtttgt
     8581 tgccaacacg catgattatt tgatgtgctt taccaatttg ggcaagtgtc attggattaa
     8641 ggtttacaaa ctgcccgaag gcggacgcaa cagccgcggc cgtccgatta acaacgtcat
     8701 ccagttggaa gaaggcgaaa aagtcagcgc gattctggca gtacgcgagt tccccgaaga
     8761 ccaatacgtc ttcttcgcca ccgcgcaggg aatggtgaaa aaagtccaac tttccgcctt
     8821 taaaaacgtc cgcgcccaag gcattaaagc catcgcgctc aaagaaggcg actacctcgt
     8881 cggcgctgcg caaacaggcg gtgcggacga catcatgctg ttctccaact taggtaaagc
     8941 catccgcttc aacgaatact gggaaaaatc cggcaacgac gaagcggaag atgccgacat
     9001 cgaaaccgaa atttcagacg gcatcgaaga tgaaaccgcc gacagcgaaa acgcactgcc
     9061 gagcggcaaa cacggtgttc gcccgtccgg tcgcggcagc ggcggtttgc gcggtatgcg
     9121 cctgcctgcc gacggcaaaa tcgtcagcct gattaccttc gcccctgaaa ccgaagaaag
     9181 cggtttgcaa gttttaaccg ccaccgccaa cggatacgga aaacgcaccc cgattgccga
     9241 ttacagccgc aaaaacaaag gcgggcaagg caatattgcc attaacaccg gcgagcgaaa
     9301 cggcgatttg gtcgccgcaa ccttggtcgg cgaaaccgac gatttgatgc tgattaccag
     9361 cggcggcgta cttatccgca ccaaagtcga acaaatccgc gaaaccggcc gcgccgcagc
     9421 aggcgtgaaa ctgattaact tggacgaagg cgaaaccttg gtatcgctgg aacgtgttgc
     9481 cgaagacgaa tccgaactct ccgacgcttc tgtaatttcc aatgtaaccg aaccggaagt
     9541 cgagaactga aaatcatctc ccgatgccgt ctgaagattc agacggcatt tattttatcc
     9601 ctcatccgtc atccggcttc tcacaatata gcggattata gtcaattaaa aacaaggggc
     9661 tgtcctagat aactagggaa attcaaatta agttagagtt gcccctatga gaaaaagtcg
     9721 tctaagccgg tataaacaaa taaactcatt gaactgtttg tcgcaggtgt aactgcaaga
     9781 acgacagcag agttagtagg cgttaataaa a
//
LOCUS       DAXHPG010000064         9795 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXHPG010000064 DAXHPG010000000
VERSION     DAXHPG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.064"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(9..1304)
                     /locus_tag="ACFM6F_001178"
     CDS             complement(9..1304)
                     /locus_tag="ACFM6F_001178"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6164593.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAIELVDERVRKHTIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFRPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1430..2317)
                     /locus_tag="ACFM6F_001179"
     CDS             complement(1430..2317)
                     /locus_tag="ACFM6F_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6164594.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATRR
                     DREAAEAYRQEKLAAEKAH"
     gene            complement(2317..3282)
                     /locus_tag="ACFM6F_001180"
     CDS             complement(2317..3282)
                     /locus_tag="ACFM6F_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6164595.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNITISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3298..4422)
                     /gene="potA"
                     /locus_tag="ACFM6F_001181"
     CDS             complement(3298..4422)
                     /gene="potA"
                     /locus_tag="ACFM6F_001181"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC6164596.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4617..4724)
                     /locus_tag="ACFM6F_001182"
                     /pseudo
     CDS             complement(4617..4724)
                     /locus_tag="ACFM6F_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4803..5072)
                     /gene="rpsO"
                     /locus_tag="ACFM6F_001183"
     CDS             complement(4803..5072)
                     /gene="rpsO"
                     /locus_tag="ACFM6F_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC6164597.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5295..6230)
                     /gene="secF"
                     /locus_tag="ACFM6F_001184"
     CDS             complement(5295..6230)
                     /gene="secF"
                     /locus_tag="ACFM6F_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC6164598.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6234..8087)
                     /gene="secD"
                     /locus_tag="ACFM6F_001185"
     CDS             complement(6234..8087)
                     /gene="secD"
                     /locus_tag="ACFM6F_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC6164599.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8299..8565)
                     /gene="yajC"
                     /locus_tag="ACFM6F_001186"
     CDS             complement(8299..8565)
                     /gene="yajC"
                     /locus_tag="ACFM6F_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC6164600.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8693..>9795
                     /locus_tag="ACFM6F_001187"
     CDS             8693..>9795
                     /locus_tag="ACFM6F_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC6164601.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKIFRL
                     DNDTAISTGSLSAAHFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2509 a         2578 c         2437 g         2271 t
ORIGIN      
        1 tgcgacgctt aacggctgtc tttccactga taatatttcg agccgaggaa agaaccgagt
       61 ttgcgcaaaa acggttgcag gataatattc ggctggcgca accgctcaaa cggcttcaga
      121 cggcattcgt cccctaaaat tgcttcggca accgccagac ctgcaatgcc tgttatcgcc
      181 atcccgtgtc cggaataacc ttgcgtataa aaaacattcg gggctaaacg tccgaaatgc
      241 gggacaaggt tggcggtaat gtcgcactcc ccgccccacg aatattcgat tttgacatcg
      301 gcaagctgcg gaaaaacttt aagcatatct tggcggacaa gctcggtcat acgcgcggga
      361 ttgtcgataa actcgttatc cttaccgccg aaaagcagtc tgccgtccgc gctgaggcgg
      421 taataatcca aaatatggcg gttgtcgcat actgccatat tgttgcggat aagccctttt
      481 gcacgcgcgc ccaagggttc tgtggcaata ataaaggtgc tgacagcaat cgccttgcgt
      541 tccaaaggcc ggaatatcgg attcaaaccg gcataagtat tgacggcgta caccagattt
      601 ttgcattcga cgctgccttc gggggtgtaa accagccaac cgttttgata aggttcaatg
      661 cgtatcatgg gagaatgctc aaaaatcttc gtaccagctt cggcagcggc gcgggcgatg
      721 cccaacgtgt aattgagcgg atggagatgc ccggacaagg gatcgaactg tgcgccttgg
      781 tacatatcgc tgtcaagctg ctgcttcagt tcagtgttat cccagagttg ataatgactc
      841 gcaccgtaat gccgttgggc gtgttcatgc cattgttgca attcttccca atgctgcgga
      901 cggacggcaa ccgtggcata accgcgctgc caatcgcaat cgatggtatg tttgcggacg
      961 cgttcgtcca ccagttcgat cgcctgcaaa gactgttgcc aaaaccattg cgcctgttcc
     1021 aaaccgacct gtttttcaat ttcctccata ccgcaggcgt aatcgctgat aacctgcccg
     1081 ccactccgtc ccgacgcgcc gaaaccgata cgcgcggctt ccaacacaac cgtttcatgt
     1141 ccctgctccg ccaagggcaa tgcagtgcac aaaccactca atccgccgcc gatgatacag
     1201 gtatcggttt tcagacggca ttgaagtttc ggataaacag tatgaggatt aaccgaactg
     1261 aaataataag aaggcagata ttcttgaaaa tcagggcgaa tcattatgtt tactttatca
     1321 ggtgtatttt cagacggcat gatatggttt gccgtaccat gccgtctgaa cgggaaatta
     1381 ctttaagaaa acaggctgac ctgataatgc ggtcagcctg ccctgtcggt caatgcgctt
     1441 tctcagcagc cagtttttcc tggcggtagg cttctgccgc ttctcggtca cgcctggtcg
     1501 cctgcctcat catccaataa ttgacgatga tgaccaatgt tccgatgatg ccgatcagga
     1561 tggttgccaa aacgttcatc tgcggatcaa gacccagctt gattttggag aaaatcacct
     1621 gcggcaatgt ggatgaaccg gggccggaga ggaatgaggt aattaccaaa tcatccaaag
     1681 acagggtaat gccgagcaga aagcctgaag cgatggcagg ggcaatcaaa ggcaaagtga
     1741 tgacaaaaaa gattttcagc ggacgcgcgc ccaaatccat tgcggcttct tcgagcgatt
     1801 ggtcaagctc aaccagacgc gaacggataa caacggtaat gtacgccata cacagcgtcg
     1861 tatgtccgag gaagatggtg aaaaagccac gatcgaagta gagatgttgt aaccattcgc
     1921 tgccctgcaa aaatatctgt acctgaataa tcagcagcag catagacaga ccggtaatca
     1981 cgtcgggcat caccataggt gcggaaatca taccggcgaa caaggtactg ccgcgaaaac
     2041 gtttaatccg cgccatcgca tagcctgcca gcgtgcccaa aacgacggca gcaagcgaag
     2101 acacaacggc aatccgcagc gacagccaag cggcttccaa gatggtgtcg ttttccagca
     2161 atgcgccgta ccacttggtc gaaaagccgc cccaaacggt taccagcttg gattcgttaa
     2221 acgaatagat gaccaaaaca accagcggga tatacagaaa cgccagcgac agtgccaaca
     2281 tcagtttcaa gaaccaagat aatttggatt tctgcattat ttggctcctt cttccaattc
     2341 gcggttttca taatgctgaa acagggcaat cggcacgacc agcagcgcga ccatcacgac
     2401 ggcgacggcg gaagccagcg gccagttgtt ttgatcgaag aacgcctgcc acaagacttt
     2461 accaatcatc aggttttccg aaccgccgac cagctcggga atgacgaact cgccgacagc
     2521 agggacgaaa accagcatgg agcctgcaat aatgccggtt ttcgacaaag gcagggtaat
     2581 cgtcaagaac gatttgaccg gccccgcgcc caaatcggaa gccgcttcaa gcaggcggtt
     2641 gtcgagtttc accagttgcg tgtatagcgg cagaatcata aacggcagat aggcgtaaac
     2701 catcaccaaa ttgagcgaaa aggcattgta gaacaaatcc aaaggctcgc tgataatacc
     2761 cattttaatc aacaggttgt ttacaatacc gttatgcccg agcagaccca tccacgcata
     2821 gacgcgcaac aggaacgatg tccaaaaggg cagcataatg gcaagcagca aaccattgcg
     2881 gacagaagga ttggcacgag aaatcgcata ggcggtcgga taaccgacca acaggcaaat
     2941 cactgtcgtc atcaatgcgg ttttgattga agaccagtag gtcatcagat agatattgct
     3001 gttttcactg tcgccgaacg ggttaagggt atgccaaaaa ttttggaaaa tatcggcata
     3061 gttttgataa ctgatggtga tattcagacg acctaaatct tcgtcgatgc tggtcagcgg
     3121 cgtaaacggc ggaatggcga tttcttgttc ggcaaagctg attttcagca cgatggcgaa
     3181 cggaatcaga aacagcacca aaagccaaat atacggtacg gcaatcacca cacgctgccc
     3241 cgggcggcgg aacagtttgt ttttcagttt attaaggttc attgcattcc ccttaaatca
     3301 acggaacagt ggcgtaggct ggttttccgg ccagctgata tacacggttt cgtcccaagt
     3361 tggcggtgta atgttgcgta cataccagta aggtgcagga acttggcttt tgacgacgcg
     3421 cccgttggcg agtttgatat ggtatatggc aaagctgccc aaataggcga tttcttttac
     3481 cgtgcctttc gcccagttgt agtcgcccaa atattcgggt ttttctttat ataaatcaat
     3541 atcctctggt cgaatactaa cccaaaggtc ctgctcgctc ggaccaccca aaccgtgatc
     3601 gatgcggacg tggttttcca aaccttcgca ttcgataacg gcatagtcgg catgatcttc
     3661 aatcaccaca ccgtcaaaga tgttggtttc gccgataaac tcggcagtga agcggctgtt
     3721 gggatagtcg tacacgtcgc tgggtgtgcc gacttgctgc aactgaccgt cagacataat
     3781 ggcgatgcgg gtcgccatcg tcatcgcctc ttcttggtcg tgcgtaacca taatacaggt
     3841 tacgccgact tgttccagcg tattgaccaa ctcaagctgg gtttgttggc gcagtttttt
     3901 gtccaatgca ccgaggggct catccagcag tagaattttc ggacgttttg ccagactgcg
     3961 tgccaaagca atgcgctgct gctgaccgcc ggacaattgg tgcggtttgc gtttagcaaa
     4021 tttggtcatc tgaaccaggc ggagcatttc ttcgacgcgc gcggcgattt cgcctttagg
     4081 cattttgtcc tgtttcagac cgaaggcaat gttttgttct acggtcatat gcggaaaaag
     4141 cgcgtaactt tggaacatca tattgatggg gcgatcatag ggtgcaagtt tggtaatatc
     4201 ctgaccatca aggataattt ttccctgatt gggactttcc atacccgcca gcatacgcag
     4261 cagtgtagat tttccgctgc cggaactgcc caaaagggcg aagatttcgt gttgataaat
     4321 gtccaagtcg atgttatcga cagcgtaatt gtcaccaaac tttttcacca aaccttggat
     4381 tttgagataa ggtttggctg aagacgcagt ggttgcggtc ataatggcaa tactccaata
     4441 aaaagacgag taccggcaaa acggattttc gaatgggtga taaaaagctg tttgattgct
     4501 ggcgggagtt aaacgtttga tgccgtctga aactcttgta aagcgcacgg gcagcatgaa
     4561 atggaacaag attccaaaga actttatatt atattagttt atgcggtttt cgggcaatat
     4621 agtggattaa atttaaacca gtacagcgtt gccttgcctt gccgtactat ttgtactgtc
     4681 tgcggcttcg tcgccttgtc ctgatttaaa tttaatccac tataaatatg gatttgagct
     4741 tgtcggaaag caacagaaaa gaaaacaccg cccatttttc tgggcggtgt cggaaagcgt
     4801 aattatttac gcagacccaa gcgggtaatc aacgcgcgat acgtatcggg ctgggtacgg
     4861 cgcaagtagg ccagcaggcg gcggcgttgg ctgaccattt tcaacaggcc gcgacggctg
     4921 tggtggtctt tggggttggc tttgaagtgg ggggtcaggt cgttgatgcg gaaagtcaac
     4981 agagcgactt gtacttcgga agagccggtg tcgccttctt tgcgttggaa atctttaacg
     5041 atttgtgctt tttgttctac ggtcagtgcc ataatgaaaa ctccaaaaat ataagaatcc
     5101 ccatagggat tcagacaagt ttgccaagcc tgaagacaac ggcaaactcc ctatgcccaa
     5161 gataggacaa cgtggcatta tgacacaatt cccgcatttc tgcacaatat tttaagaccg
     5221 tggaagtaac cgttaaaaat gccgtctgaa gcattgtctg cttgagacgg caatgttcag
     5281 acggcatatg cgcttcaaac cacgatttct tctttcttct tcggttcttt accgatattg
     5341 tcgcggctca aaccgaacat tagcagaagc gggctggcaa ccaatacgga agaataaatg
     5401 ccgaacacga tgccaatggt caacgccata gaaaagccgt gcaaggccgc accgccgaac
     5461 accagcatgg atacgaccat cgcctcggtc gaaccgtggg taatgatggt gcggctcatc
     5521 gttgcggtaa tcgcgttgtc gatgacttcc ggcacggcat gtccgcgcat cgccggcttg
     5581 cggaagtttt cacggatacg gtcgaagacg acgacggatt cgttcacaga atagcccaat
     5641 acggcaagga tacccgccaa gacggtcagc gaaaattccc attggaagaa ggcaaagcag
     5701 ccgagaataa tcacgatgtc gtgcatattg gcgataatgg cagatacggc aaaacgccat
     5761 tcaaaacgca tcgacaggta aataatgatg ccgataacga caaaacctaa agccatcaat
     5821 ccattactta ccaattcctc accgacttgc gggccgataa attcgacttg gcgcaaggta
     5881 acgtcgggac tgtctttttt cagcaaatcc ataacctgat tggacaactg tgcggaagta
     5941 acaccttctt tgttcggcag gcggatcatg atgtgtttgt tcgtacccaa tgcctgaacc
     6001 tgtacatcac ctattttcag cgtatcgagg cgttcgcgca tcttattgac atccgcaccc
     6061 tgctgatatt ggacttccat taccgtaccg ccggtaaatt cgacagagaa attcagacct
     6121 ctggtaacca aaaagaatac ggcggcaata aaggtaacca acgaaataaa ggtcgtcagt
     6181 ttgccgtagc tcataaacgg aatatcgcgt ttgattttaa agagttccat agcttactcc
     6241 ttacctgccg cagtttcggc ttcaggtttc cataccgcac cgatggaaat attctgcaat
     6301 ttgcgtctgc gtccgtacca cagattgacc aacgcacgga atacgacgac ggaggaatac
     6361 atcgaagtca gaatacccaa acagtgtacg accgcaaaac cgcgtaccgg gccggaaccg
     6421 aataccaaaa gcgcgatacc ggcaatcagc gaagtcaggt tggaatcgac aatggtcgcc
     6481 catgcgtgtt ggaaaccgag attgattgcc tgctgcggcg gcacgccggc acgcaattct
     6541 tcgcggatac gttcgttaat caagacgttg gagtcgattg ccatacccaa agtcaacgcc
     6601 agcgcggcca tacccggtaa cgtcaacgtt gcctgcatgg cagacaaaat accgattagg
     6661 aacagtatgt tggcactcaa tgcaatggta gaaaagaaac ccatcagacg atagtaaacc
     6721 accatgaatg cagcaacgat ggcaaaaccc cataaagtcg aatggaagcc tttttcgatg
     6781 ttctccttac ccaaagacgg accgatggta cgttcttcga caatctgcat cggcgcggca
     6841 agagaaccgg cacgcaacag caaagacgta tcattggctt cggctgtcgt catgcttccg
     6901 gaaatttcca cgcgtccgcc ggtaatggca gtacggataa ccggcgcggt tacaacctcg
     6961 gattttcctt ggtcgatcaa aaccatcgcc atgcgtttgc cgacatttgc ggcagtcagt
     7021 tcgccaaaaa tgctgccacc cgcgctgtcc aagctcagac tgacggcagg tgcgcccatt
     7081 tggtcgaaac tcggttgcgc atcgttgatg ttgtcgcccg tcagctcgac ctgtttgctg
     7141 attagcagag tttcgggacg atctccgccg cttgaaagca gctcataacc gctcggcacg
     7201 ttgccttcca atgcctcgcg caacttggca ggatcgtcct ccaccatacg caattccaaa
     7261 gtcgcggtac ggccgatgat gtcttttgcc ttggcagtat cctgaacgcc cggaagctgc
     7321 acgacgatac ggtcggcacc ggactgctgg atgacgggtt cggccacgcc caactcgttc
     7381 acacggttgt gcagggtagt gatgttctgt ttgaccgcat cggaacgcac tttattgacc
     7441 gcctcttccg aaagtgtcaa gacgatattg ctgccgtctg aattcagcgt tgcttcagga
     7501 aacagcttgc gtaactgcgg cagtgccttt tgcacatcac ctgcatcctg caaagggacg
     7561 gtcaggctgt ttccagcctg acgcaccgtg ccgctgcgga ttttttcgcg gcgcagttcg
     7621 cggcggatgt cgcccgaata acgttcaaac gttttctgca tcgccgcttt catgtccacc
     7681 tgcatggtga aatgcacacc gccgcgcaag tccagaccca aaaacatcgg attggctttg
     7741 attttcgcca tccactcagg actgtctgcc aaaaggttga gtgcggtaat atacccttcg
     7801 cccaaagtat tttcgatgac gtcgcgcgct ttaagctgcg tttcggtgtc tttgaaacgc
     7861 actttcagtg aattgtccac aacaaacatc ccgtcggtct gaatacccgc gttttgcagc
     7921 gcggcatcca ctttggattg agtctgttcg ttgatgatga tggcttgtcg gttggtcgat
     7981 acctgcacgg cgggtgtttc gccgaatagg ttgggcagcg aatacactgc ggcaaccgca
     8041 atcgtgaaca caatcagcag atatttccat aaaggataac ggttcatcat tgttccttaa
     8101 tggttggaac cccacccttt cggtggtgtc ggaatcgggc tatttcagaa gaggcaaaaa
     8161 cccttcccag ccaggcaaga ccggaaggcg gcatcttgaa tatgccgccc tgcgtgtcgg
     8221 aacatggtca agccttcggt tggaattcaa aacaaagtgc cgcattcggg ctttccagat
     8281 gcggcttgtc ggcacaaatc aatcgacttt tgcggcaatc gcattgcgtt ctacttcgac
     8341 ctcgattttt gtaccctgtc cgatatccac ggtaaaaaac tgttcgccga ctctggttac
     8401 cttacccttg aaacctgccg ccaagaccac tttgtcgccg actttcaagg cggcaagcat
     8461 tgcctgatgc gctttgaatt tcttttgctg cggacgcatg atcaggaagt agaacaccac
     8521 cataatcaac actaaaggag caaattgtgc aacagcttga ttcataattt atccgttctt
     8581 tctaatatgg ttgaaaatcg agaggggtat ataataacat aagaccgtaa acaatatatc
     8641 gggtttgccg tccgtaccga ccgtataccg cagcctgccc gtccacaaac ccatgtcctt
     8701 gacccgccta atcttgaaat tctatgcact gttgcgcctt tttttgggca aaaacgcccg
     8761 caccgcatgg atttcgcatc ccgcctgtgc cgggcacgaa cccggcgcaa accatcccga
     8821 ttcgcccgac cgcatcctct gcatcgagca ggcattgcgc cgcgccggta tttggcagca
     8881 cctccaaacc atagaggcgg aagaaatcag cgatacgcgc ctcgcacttg tccactcgag
     8941 caaatatctg aaccgtttgg aatcttgcct gccccaaaaa ggcaagattt tccgcctgga
     9001 taacgacact gcaatcagca caggctcgct gtctgccgca cactttgccg ccggttcggc
     9061 agttcaggca gtcgacatgg tcatgaaccg taaagcatgg catgcctttt gcgccgcccg
     9121 cccgcccgga caccatgcgg gcagcggcaa agccggcgga ttctgcctgc tgaacaacgt
     9181 tgccgccggc gtcatgcatg ccattgccga ataccgcctg aaacgcattg ccgtcatcga
     9241 tttcgatgtc cactacggcg acggtacggc agaaatattc aaagacgatc cgcgcatcct
     9301 gtttttcaac ctgtttgaaa ccgacctttt ccccttcccc gaaaacaacg atatgcccga
     9361 cggcggcaat atggtgcacc tgcccttgcc gccgggaacg ggcagccgca cattccgcga
     9421 agccgtccgc aggcagtggc tgccccgact tgccgcattc aaacccgaac tggtgctgct
     9481 gtcggcagga ttcgacgcac accgtctaga cgaatcgggc aggctcaacc tgcacgaggc
     9541 ggattttgcc tggctgacac acaaaatcat tcagacggca tcgggctgcc ccggcaaaat
     9601 cgtatccgta ctcgaaggcg gctacaccct tgaacctctg gcacaatctg ctgccgagca
     9661 tatccgcgtg ctggcagggc tgggcaaatc cgatgccgca accgcctatc agaaaacact
     9721 ggacccaaca aaaaaacggt ttgccaaacc gaaaaccgga caggtgcgcc aacctaccca
     9781 atccgaccgt tacga
//
LOCUS       DAXHPG010000065         9761 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXHPG010000065 DAXHPG010000000
VERSION     DAXHPG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.065"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(163..1209)
                     /gene="adhP"
                     /locus_tag="ACFM6F_001188"
     CDS             complement(163..1209)
                     /gene="adhP"
                     /locus_tag="ACFM6F_001188"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC6164602.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1333..1461)
                     /locus_tag="ACFM6F_001189"
     CDS             complement(1333..1461)
                     /locus_tag="ACFM6F_001189"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164603.1"
                     /translation="MPSGHSFRRYLYEFAVRFFKLETCRPSSGNRPDAAPFQAVII"
     gene            complement(1492..1881)
                     /locus_tag="ACFM6F_001190"
     CDS             complement(1492..1881)
                     /locus_tag="ACFM6F_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6164604.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLTEVR
                     TTLLHNAQTMERYYRQKGTFKTYDQTKLKQNEYFKITLSEVSPDHFTLQADPNPMTNE
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            2107..3285
                     /locus_tag="ACFM6F_001191"
     CDS             2107..3285
                     /locus_tag="ACFM6F_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC6164605.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITEAVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNSDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3351..5285
                     /locus_tag="ACFM6F_001192"
     CDS             3351..5285
                     /locus_tag="ACFM6F_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC6164606.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5603..6385)
                     /locus_tag="ACFM6F_001193"
     CDS             complement(5603..6385)
                     /locus_tag="ACFM6F_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC6164607.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAAKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6524..8713
                     /locus_tag="ACFM6F_001194"
     CDS             6524..8713
                     /locus_tag="ACFM6F_001194"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC6164608.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPQGMRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSIQTTFLDEPPLPESWRDLLSFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     VKLIENWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPVVLGSATPSLESWHKVQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRVRQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            complement(8760..9761)
                     /locus_tag="ACFM6F_001195"
                     /pseudo
     CDS             complement(8760..9761)
                     /locus_tag="ACFM6F_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675858.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="NnrS family protein"
BASE COUNT         2264 a         2522 c         2844 g         2131 t
ORIGIN      
        1 ccgccttgtc ctgatttttg ttaatccact ataatgacgt gttgcgataa gggttttcag
       61 acggtatttc aaacagaaca gattaaaatc aaatccaaat ccataaaaaa tgccgtctga
      121 acagcgttca gacggcattt cgatgtgtat cgccacgtca aatcagtggt ggtggccgca
      181 gccgcattct tttttcatat cgattaccat acgaccggtg attttgcctt cgcgcatttc
      241 ttggaaaatg gcgggtgctt catccaaagc acgcagttgg actttcggca caaccaaacc
      301 ttccgcgccg aattggaagg cttcttccaa atctttgcgc gtgccgacca aagagccgac
      361 cacttcgatg ccgtccaaaa ccaaacgcgg gatggacaaa tccatcgatt ccggcggcag
      421 tccgatggca accacacgtc cgcccgcgcg gacgcaattc acggcagagt tgaatgcggc
      481 agcagatacg gcggttacga ccgcagcgtg tgcgccgcct gttttttcct gaatcacttt
      541 ggcagcgtcc tctttggcgg cgttgacaac caaatccgcg ccggtttctt tggcaaacgc
      601 cagtttgtcg tcgttgatgt cgatggcgac aacgtgcgcg ccgaatactt ttttcgcgta
      661 ttggaccccc aagttgccca aaccgcccgc gccgtagatg gcaatccact gtcccggacg
      721 aacactggaa actttaatgg ctttataagt ggtcacgccg gcacaagtaa tgctcgaagc
      781 ttgcgcagga tccaaacctt cagggacttt gaccgcgtaa tcggcactca cgatacagtg
      841 ggtcgccatg ccgccgtcgg cagtatagcc agcgttcaat acagagcggc acaaggtttc
      901 gcggccggta ttgcagtatt cgcaagaacc gcagctttgg aacagccagg cgatgctgac
      961 gcggtcgccg actttcagat ttttcacacc gtcggcaact tctttaacca aaccgatgcc
     1021 ctcgtgtccc agcacgcggc ccggtttttc gccgtagtcg cctgccgcaa cgtgcaggtc
     1081 ggtgtggcac acgccgcaat attcgacttc gaccaacgcc tcgccgtatt ccaacgggcg
     1141 aacctcgcgt tcgactattt ccacatcgcc cgctacattt ttattcacaa caactgcctg
     1201 cattttcatg attgcgctcc ttagttacgg caaaaaaacc tgtaaacgga atgttgtccg
     1261 atataagggt aagcatacgc ttccgcatct cacaggtcaa gtggtatgtt gttgaaaaat
     1321 atagattata tgttatatta taacagcttg gaaaggcgcg gcatcggggc ggttgccgga
     1381 tgaggggcgg caggtttcaa gtttgaaaaa ccggacggca aactcgtaaa gataccgtct
     1441 gaagctgtgt ccggacggca tctttacggg tttgcgggct tcggcggagg attagtcgaa
     1501 gccggggcag gattggtttg taccggaagc ggcaatggta ccgccgtcgt tgagcgtaac
     1561 gacgcaggtt tcgccttcgt tggtcatcgg attggggtcg gcctgaaggg tgaagtggtc
     1621 ggggctgact tcgcttaagg taatcttgaa atattcgttt tgtttcagtt tggtttggtc
     1681 gtaggtttta aacgtccctt tttggcggta gtaacgttcc atggtctgcg cgttgtgcag
     1741 cagggtcgtc ctgacttccg tcaggcggac gcgccggatg taggttttat aggaagggta
     1801 ggtgatgagc gtcaggatgc cgaggatggc gacggcaatc atcagctcga gcagcgtaaa
     1861 gcctttttga acgtttttca tagcaatgtg tttccatttg tttgtcggtc ggcgacatta
     1921 taacgccgta tcggaaatgg cggaatatgt aaacggattg aaattttcgg gaaagcagat
     1981 tgtataagcc atttaaaaca aatggttatt tttattgtcg gcagtttgcc gccttggatg
     2041 gggcagggac ttgcggtaga atccgcttcc gatttatggg attgacgcat acagagaatt
     2101 gaaaacatgg caaaaatgat gaaatgggcg gctgttgcgg cggtcgcggc ggcagcggtt
     2161 tggggcggat ggtcttatct gaagcccgag ccgcaggctg cttatattac ggaagcggtc
     2221 aggcgcggcg acatcagccg gacggtttct gcaacagggg agatttcgcc gtccaacctg
     2281 gtatcggtcg gcgcgcaggc atcggggcag attaagaaac tttatgtcaa actcgggcaa
     2341 caggttaaaa agggcgattt gattgcggaa atcaattcga cctcgcagac caatacgctc
     2401 aatacggaaa aatccaagtt ggaaacgtat caggcgaagc tggtgtcggc acagattgca
     2461 ttgggcagcg cggagaagaa atataagcgt caggcggcgt tatggaagga aaacgcgact
     2521 tccaaagagg atttggaaag cgcgcaggat gcgtttgccg ccgccaaagc caatgttgcc
     2581 gagctgaagg ctctaatcag acagagcaaa atttccatca ataccgccga gtcggaattg
     2641 ggctacacgc gcattaccgc aacgatggac ggcacggtgg tagcgattct cgtggaagag
     2701 gggcagactg tgaacgcggc gcagtctacg ccgacgattg tccaattggc gaatctggat
     2761 atgatgttga acaaaatgca gattgccgag ggcgatatta ccaaggtgaa ggcggggcag
     2821 gatatttcgt ttacgatttt gtccgaaccg gatacgccga ttaaggcgaa gctcgacagc
     2881 gtcgaccccg ggctgaccac gatgtcgtcg ggcggctaca acagcagtac ggatacggct
     2941 tccaatgcgg tctactatta tgcccgttcg tttgtgccga attcggacgg caaactcgcc
     3001 acggggatga cgacgcagaa tacggttgaa atcgacggcg tgaaaaatgt gctgattatt
     3061 ccgtcgctga ccgtgaaaaa tcgcggcggc aaggcgtttg tgcgcgtgtt gggtgcggat
     3121 ggcaaggcgg cggaacgcga aatccggacc ggtatgagag acagtatgaa taccgaagta
     3181 aaaagcgggt tgaaagaggg ggataaagtg gtcatctccg aaataaccgc cgccgagcag
     3241 caggaaagcg gcgaacgcgc cctaggcggc ccgccgcgcc gataaacgaa tatgccgtct
     3301 gaacacggaa acggtttcag acggcatttg ttattgattt acggaatatt atgagcttga
     3361 tcgaatgtaa aaacatcaac cgctatttcg gcagcggcga gaaccgcgtc catattttga
     3421 aagacatcag cctgtcgata gagaagggcg attttgtcgc catcatcggg cagtccggtt
     3481 cgggcaagtc cacgctgatg aacatactcg gctgtttgga taccgccggt tccggttcgt
     3541 accgaatcga cggcatcgaa actgccaaaa tgcagcctga cgagctggcg gcattgcgcc
     3601 gcgaacgctt cggtttcatc ttccaacgct acaacctctt aagctcgctg accgcaaggg
     3661 acaacgtcgc gctgccagcc gtgtatatgg gcgcgggcgg caaagagcgt tccgcgcgtg
     3721 cggagaagtt gttgcaggat ttgggtttgg caagcaaaga gggcaacaag cccggcgaac
     3781 tctcgggcgg acagcagcag cgcgtctcca tcgcccgcgc cctgatgaac ggcggagaaa
     3841 tcatcttcgc cgacgagccg accggcgcgc tcgataccgc cagcggcaaa aacgtgatgg
     3901 aaatcatcca taagctgcac gaagccgggc ataccgtcat tatggtcacg cacgaccccg
     3961 gcatcgccgc caatgccaac cgcgtcatcg aaatccggga cggcgaaatc atttccgaca
     4021 cctcgaaaaa tccagaaatc cccgcaagca atgtcgggag gattcaggaa aaagcctcgt
     4081 ggtcgtttta ttacgaccag tttgtcgaag ccttcagaat gtcggtgcaa gcagtattgg
     4141 cgcacaaaat gcgttcgctt ctgacgatgc tcggcatcat catcggtatc gcttcggttg
     4201 tctccgtcgt cgcattgggc aatggttcgc agaaaaaaat ccttgaagac atcagttcga
     4261 tagggacgaa caccatcagc atcttcccgg ggcgcggctt cggcgacagg cgcagcggca
     4321 ggattaaaac cctgaccata gacgacgcaa aaatcatcgc caaacaaagc tacgttgctt
     4381 ccgccacgcc catgacttcg agcggcggca cgctgaccta ccgcaatacc gacctgaccg
     4441 cttctttgta cggcgtgggc gaacaatatt tcgacgtgcg cggactgaag ctggaaacgg
     4501 ggcggctgtt tgacgaaaac gatgtgaaag aagacgcgca ggtcgtcgtc atcgaccaaa
     4561 atgtcaaaga caaactcttt gcggactcgg atccgttggg taaaaccatt ttgttcagga
     4621 aacgcccctt gaccgtcatc ggcgtgatga aaaaagacga aaacgctttc ggcaattccg
     4681 acgtgctgat gctttggtcg ccctatacga cggtgatgca ccaaatcaca ggcgagagcc
     4741 acaccaactc catcaccgtc aaaatcaaag acaatgccaa tacccaggtt gccgaaaaag
     4801 ggctgaccga tctgctcaaa gcgcggcacg gcacggaaga tttcttcatg aacaacagcg
     4861 acagcatcag gcagatagtc gaaagcacca ccggtacgat gaagctgctg atttcctcca
     4921 tcgccctgat ttcattggta gtcggcggca tcggcgtgat gaacatcatg ctggtgtccg
     4981 ttaccgagcg caccaaagaa atcggcatac ggatggcaat cggcgcgcgg cgcggcaata
     5041 ttttgcagca gtttttgatt gaggcggtgt taatctgcgt catcggcggt ttggtcggcg
     5101 tgggtttgtc cgccgccgtc agcctcgtgt tcaatcattt tgtaaccgac ttcccgatgg
     5161 acatttccgc catgtccgtc atcggcgcgg tcgcctgttc gaccggaatc ggcatcgcgt
     5221 tcggctttat gcctgccaac aaggcggcca aactcaatcc gatagacgca ttggcgcagg
     5281 attgaggttg gacaaagatg ccgcctgaag ctgcagaacc ggtcattttg gagcagaaac
     5341 ttattggaca aaaacggttt cttagattct acgttccaaa ttcccacttg cgtgggaatg
     5401 acggcggtgt gtttcgtatt gttcaattta ttattttcaa tcattcaatg ggttaggatg
     5461 tgtttgttgg cttgctgtac tggtttaaat ttaatccact ataaatttaa tccactataa
     5521 ctttcagtgc ggattggttt tcaggcggca tttcccgcaa aaaaggcttg gaattttcca
     5581 agccttttgt tttgcggatg gattattgat ttttgcggat gatttcctcc agttggggca
     5641 tcgggctgta gccgctttgg ctgcgcccgt tggggaagac gagggtcggc gtgccgttga
     5701 agccgaattg ttcgcccaag gaagtggttt ccgcgacggg attgtcgcag atgctgccgc
     5761 cgaccgggaa tttgccttta cgcatccaat ccgtccacgc tttggcgcgg tcgggctgac
     5821 accataagat ttgcgccttg cgcgcggcat cggggtgcag gccggcaatg ggcatcataa
     5881 agctgtaaac cgtcacgtcg gtcatttttt caaactcgtg ttccaagcgt ttgcagaacg
     5941 gacaatcggg gtcggagaag acggcgactt tcagcttgcc gttgccgcgc acttctttga
     6001 tggctttgtc caaaggcagg gaggcgaagt cgattttgtt caaatcggcg gcgcgttctt
     6061 cggtcaggtt tttgcgcgtg tcgatgttga tgagttcgcc gacgaacata tagccgcctt
     6121 cggcatcggt gtagataatc tgtctgccgc tgacgacgac ttcgtaaatg cctttgaccg
     6181 gtgtttcgct aacgctcaac actttcaaat cttgggcgga ataggttttt tccaaacgcg
     6241 ctttcaaaga ggcggcaacg gatttgccgg cggactcggc tttggcggcg ggttcggcgt
     6301 tggcattgga aacgggcgtt tgcccgcaag ccagcagcgg gaggacggta aagggggtca
     6361 agattttgat taacttggtt ttcatataaa gatgattgcg cgtgttggaa aagcggaatt
     6421 gtatcaaatc tctgttgcgc ctgcattgcg cctaggctca atttatcgtc tgaaaatagc
     6481 ttccgcctgt taaaatacgc aaaaaaatga tttgcctgcc tgtatgatct accaccgtat
     6541 cgccgtaaac gtgccgcttt cagacggtct tttaacttat tcccattccg agccgctgcc
     6601 gcagggaatg cgggtgcttg tgccttttcg caataaaacc gtggtcggga tggtgtggga
     6661 aacggatatt gcgccggata tggatacggc gcggattttg agtattcaga cgaccttttt
     6721 agacgagccg ccgttgcctg aaagctggcg tgatttgctc tcgtttacgt cgcgttatta
     6781 ccactatccg acggggcagg cggtgtttgc cgcgctgccg cagggtttga aggaaacgcg
     6841 cgcggtggaa atgccgcagc cgccgttgtt ttacgcgctg aacgaagcgg gcagggcgca
     6901 aacgccgccg ccggcgcggt tcaacaaaaa agcggctttg tgggacgcgc tgctgtcggg
     6961 cgaaatgacg atggcagcgt tgaagcaggt aaacgcgcag gcggtgaaat taatcgaaaa
     7021 ttgggcggaa cagggttgga ttgaaacgac ggaagcggcg aagcccgttt taaggtcgtg
     7081 taatgggcag gcttcgcact ctgaatttgt attaaatgct gaccagcaga aggcttccga
     7141 tgccattcag acggcattgg gacgcttcca gccgtttctg ctgtacggca tcaccggcag
     7201 cggcaagacc gaggtgtatt ttgatgcgat ggcgaaagtg ttggctcagg ggcggcaggt
     7261 gttgtttctg ttgcccgaaa tcaacctcac gccgcagctt ttgaagcggg tagaaaaccg
     7321 ttttgccgac gtgccgaccg ccgtgttgca cagccagatg gcggcaggca ggcgcacgca
     7381 agattatttg cgcgcgatgt tggggcaggc gaaattagtc atcggcacgc ggctggcggt
     7441 gttcacgccg atggatgatg tcgggctgat tgtggtcgat gaggaacacg acggctcgtt
     7501 caaacaagac aacgaattac gctaccacgc ccgcgatttg gcggtgtggc gggcgaagca
     7561 gagcggctgc cccgtcgtgc tgggtagcgc cacgcccagc ttggagagct ggcacaaggt
     7621 gcaaagcggc gcgtaccgcc tgctgcaact gaccgagcgc gcccataccg ctgcgcaact
     7681 gccgcaagtg gacatcctca acgtaggccg tctgaaacta gacaatggct tctcgccgca
     7741 agccttgcag cttttgaaac agaattttga agcgggcggt atgtcgctgg tgtacctcaa
     7801 ccgtcgcggc ttcgcgcccg cgctgttttg cggcgactgc ggccatacct tcggctgccc
     7861 gaactgctcc gccaaaatgg tgctgcacca acgcgtccgc caactgcgct gccaccactg
     7921 cgaccaccgc gaacccatac cgttcaaatg ccccgactgc ggaaatcaag acctgaccgc
     7981 cgtcggacac ggcacgcagc gcgtcgaaga aaccctgcgc gccttcctgc ccaaggcagc
     8041 cgtcgtccgc gtggacaggg acagcaccgc gcacaaaaac gactgggcgg atttgtaccg
     8101 ccgcatcgcc gacaacgaaa tcgacattct ggtcggcacg cagatgctcg ccaaaggcca
     8161 tgactttgcg cggctcaacc tcgttatcgt attgaacgcc gacggcagcc tttacagcgc
     8221 ggatttccgc gcgccggaaa gactgttcgc cgagctgatg caggtgtccg gcagggcggg
     8281 gcgcgccgac aaacccggca aggtgttgat acagacccaa ctgcccgaac atcccgtctt
     8341 cgccgccgtc aaagcgcagg attatgccgt gtttgccgaa aacgaattga acgaacggca
     8401 aatgttcgcc atgccgccct tcggttttca gaccgccgtc cgcgcggacg cgccgcgtgt
     8461 tgccgatgcg ctggaatttc tcaatgccgc caaagaaacc ctcgccccgc tgttgcccgc
     8521 aagcgtttcc cagtttggcg ccgcccccat gctgatggtg cgcctcgccg aacgcgaacg
     8581 cgcgcaaatc ttcctcgaat ccccgtcccg acaggatttg caccgtgccg tgagtttgtg
     8641 ggtgcaggtg ttgcagcaaa accgcgacgg caaaattaga tggtcggtgg atgtcgatcc
     8701 gcaggaggct tgattatcgg caatacgatg ccgtctgaaa accgtttcag acggcatttt
     8761 tattccggat cgtctgtaaa cgcgttcgcc cgaaagatcg gtacgaatgt cagcaggtaa
     8821 agcacgaaca cggcggcggc gaaaaggatg gggacggcga tgcggcagag tttcgggtag
     8881 tcgagcttgg taaagccgct gtgccacagt ccggcagtca gccacaccat catcacgccg
     8941 cccatcatgc cgccgagggt aatcaggtgc aggggcgcgg aggcaggcag gttttgcaat
     9001 ttcgccgcgc ctgtccacaa atagcctgcg gcggcaaaaa gttggagcag gtaataagtg
     9061 cggacgtagt gtttgcgtaa gagttcgtga tggtgcagtt cgcgcagctt ggcgagcaag
     9121 atgaagccga cggcgagcgc ggtaaaaccg gcggtttgcg cgggcagcca aagttcggcg
     9181 gcggcgtgca gcagcaggaa ggtgatggcg atgtttttat agacaacgtt ggggatgaat
     9241 acagggtctt tcagacggca ttttttgagg gcttccgcgc ccagaaggac actgacgcgg
     9301 acggatacga acatgaccgc cgccatattc aaatgcactt gcgcgcgcaa caggttcaaa
     9361 tcgccggttg cagcgtatgc cgtctgaaaa acagtgaacg cggcaagcag cattaagagg
     9421 gcgaagttgt cggtgtttcg gtctagccaa atcagccggg cgcagaacag cagcaacacc
     9481 agccaatagg cggcgacgaa aaacgaggca gtttgcggcg aaaagggcag tatagcggat
     9541 gcggcgagca ataatgccgc catcaaagtc gcgacaggtt tcaggttact cgaaaaaacc
     9601 cgtccagtcc aacaaagccg cagtcaggaa accaccatat gctgcaggca gcatgagttc
     9661 taagaagatt tggcggtgca ggacgacggc accggggctg atgaaaaaca ccaacgcgcc
     9721 gagtatggca agcactgtcg cgccgacgaa aaacgggcgc a
//
LOCUS       DAXHPG010000066         9680 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXHPG010000066 DAXHPG010000000
VERSION     DAXHPG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.066"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(35..760)
                     /gene="aat"
                     /locus_tag="ACFM6F_001196"
     CDS             complement(35..760)
                     /gene="aat"
                     /locus_tag="ACFM6F_001196"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC6164609.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFLRDGWFFWYAVGPRAVIVPERLHVPRSLEKTLRNCSYRVTVNGCFAEVVAHC
                     AAAVRPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLASLGVELIDCQQDTGHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(830..1264)
                     /gene="fur"
                     /locus_tag="ACFM6F_001197"
     CDS             complement(830..1264)
                     /gene="fur"
                     /locus_tag="ACFM6F_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC6164610.1"
                     /translation="MEKFSNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1491..1868
                     /locus_tag="ACFM6F_001198"
     CDS             1491..1868
                     /locus_tag="ACFM6F_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6164611.1"
                     /translation="MNKTLILVLSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPMLRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            1878..2687
                     /gene="dapB"
                     /locus_tag="ACFM6F_001199"
     CDS             1878..2687
                     /gene="dapB"
                     /locus_tag="ACFM6F_001199"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC6164612.1"
                     /translation="MTPLKIAIAGVNGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2801..3211)
                     /locus_tag="ACFM6F_001200"
     CDS             complement(2801..3211)
                     /locus_tag="ACFM6F_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164613.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFVYGYATKSQDINIDNTLELGELKQDDEILDYGGA
                     LELIGGRYDFPTAFRIDIVCREIELEFLDQEGFN"
     gene            complement(3213..3446)
                     /locus_tag="ACFM6F_001201"
     CDS             complement(3213..3446)
                     /locus_tag="ACFM6F_001201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164614.1"
                     /translation="MKQHVLQKKGGEIVINGTSNNKYLRGIPNEGELARVGLRLKYNG
                     PLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(3467..4621)
                     /gene="lpxB"
                     /locus_tag="ACFM6F_001202"
     CDS             complement(3467..4621)
                     /gene="lpxB"
                     /locus_tag="ACFM6F_001202"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC6164615.1"
                     /translation="MVDKKSPLIAVSVGEASGDLLGAHLIRAIRERCPQARFTGIGGE
                     LMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVGIDAPDF
                     NLGVAEKLKRTGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYLDAGGR
                     AEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVFFQTALL
                     LLERYPAARFLLPAATAATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAADAVLVTS
                     GTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELLQHDAV
                     PEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4687..5679)
                     /locus_tag="ACFM6F_001203"
     CDS             complement(4687..5679)
                     /locus_tag="ACFM6F_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6164616.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKVGLPPDLAQFAVMLENGTKM"
     gene            6172..8931
                     /locus_tag="ACFM6F_001204"
     CDS             6172..8931
                     /locus_tag="ACFM6F_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC6164617.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAERANEAEIQSRNVQPAATVADAAPSETEMQTGKRRRNGSRSE
                     RSQTAPETAAVAETAVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         2441 a         3073 c         2274 g         1892 t
ORIGIN      
        1 ccatatttga acctgaccga agcataagcc gccatcaaag ccccttgcac gccacttcgc
       61 gccgcccgat ttcctctttc aacggcacgg cgttcaacat ccgcagacgt tcggcaaaat
      121 ccgcaaacgg cagcagctcc gaaccgaaac ggcgcatatg ccccgtatcc tgctggcagt
      181 ctatcagttc cacacccaaa gacgcgagaa acggcacggc gcaggcaaac gcgattttcg
      241 acgcatccgg ttgtaatgcg aacatcgatt cgccgtaaaa caccctgccg atctgaacgc
      301 cgtaaaaccc gcccgccaac ctcgttttac cgttttcatc gggataatgg cactcaaacg
      361 aatgcgcgta tcccatttcg tgcagcttca aatatgccgt ctgaaactcg ggcgcaatcc
      421 aagttccgtc ctgattcggg cgcaccgctg ccgcacaatg cgcgaccact tccgcaaaac
      481 agccgttgac cgtaacccga tagctgcagt tgcgtagcgt tttctccagc gagcgcggaa
      541 cgtgcagacg ttcgggaaca atcaccgcac gcggcccgac cgcataccaa aaaaaccaac
      601 cgtctcgcaa aaaccaagga aacacgccgt tccgatacgc ctcgagcagc cgccccgcgt
      661 ccaaatccct gctcacgccg accagcccgt cgcaccgtgc caaagcatag gcaggatcgg
      721 gaaaggcata attgtcaggg gcaagcagcg gaatacgcat ggcagttctc caaaaacaca
      781 gacggcatcc aaaatcatga atgccgtctg aacaacaaac cgtccggatt taacgtttgc
      841 ccttggcctg acagtcgctg cacacgccgt acatataaag cgcgtgatcg acgatgcggt
      901 agccgttttc ctcggcgatt ttgtcttgca gggcttcgat ttcgggattg tggaattccg
      961 ttacctcgcc gcacttcaca cagacgatat ggtcgtggtg gtcgcccttg tccaactcat
     1021 aaaccgcctt gcccgtttca aaatggtggc gttgcaaaat gcccgcctgc tcaaattgcg
     1081 tcagcacgcg ataaatcgtc gccacaccga tttccacacc ctcttccaac aaaatacggt
     1141 acacatcctc ggcactcaaa tgctcttccg catgagtctc gaacaaatcc aaaatcttca
     1201 aacgcgggcc ggtaactttc agtccactgt ctttcagttg cgcaatgttg ctgaattttt
     1261 ccataatatt caatatccct gtaaaataat agacgctata atacgcaatt tcagcctgct
     1321 tgcccactat cacaccatag cagtttgcaa tagcaaaacc gcaacggcgg acgggcagtc
     1381 ggacacagac agggtgaaat accgcttaag cgtatgatta tcgttcgctt ttataaaata
     1441 ttcaagcagt gctacactag acatcccgat ttgcacagaa aggttctccc gtgaacaaaa
     1501 ccctcatact cgtcctttcc gccctgttca gcctgaccgc gtgcagcgtc gaacgcgttt
     1561 cactgttccc ctcgtacaaa ctcaaaatca tacagggcaa cgaactcgaa ccccgcgccg
     1621 ttgccgccct gcgccccggc atgaccaaag accaagtcct gctcctgctc ggcagcccca
     1681 tgctgcgcga cgcattccat accgaccgct gggactatac cttcaacacc tcccgcaacg
     1741 gcatcatcaa agaacgcagc aatctgaccg tctattttga aaacggcgta ctcgtccgca
     1801 ccgaaggcga cgtcctgcaa aacgctgccg aagccctcaa agaccgccaa aacacagaca
     1861 aaccataagg aacacacatg acaccgctca aaatcgctat cgccggcgtc aacggccgca
     1921 tggggcgcgt attggttgaa gccgtcaaca accatcccga caccgtcctt tccggcgcgc
     1981 tcgaacactc gggctcagaa gccctcgggc tggacgcagg ctacgccgtc ggactcaaaa
     2041 ccggcatcgc catttcagac gacgttgacg ccgttctcgc acaaagcgac gtactcatcg
     2101 acttcacccg ccccgagccc accctcaaac acctgcaaaa atgcgttgaa aaacaagtca
     2161 acatcatcat cggcacaaca ggcttcgacg atgcgggcaa agccgctatc cacactgccg
     2221 ccgaacaaac aggcatcgtt ttcgccgcca acttcagcgt cggcgtcaac ctcaccttcc
     2281 acatcctcga caccgtcgca cgcgtattaa acgaaggcta cgacatcgaa atcattgaag
     2341 gccaccaccg ccacaaagtc gatgccccca gcggcaccgc gttacgcatg ggcgaagtca
     2401 tcgccggcgc gctcggcaga gacctcaaac aatgcgccgt ttacggccgc gaaggccaca
     2461 ccggtccgcg cgatccgtcg accatcggct ttgccaccgt ccgcgcaggc gacatcgtcg
     2521 gcgaccacac cgccctcttc gccaccgacg gcgagcgcgt ggaaatcacc cacaaggcca
     2581 gcagccgcat gacctttgcc gccggtgccg tccgcgccgc agtttgggta aacggcaaaa
     2641 cgggtttgta cgatatgcag gacgtactcg ggctgaacaa ccgttaaccc ccatacaaaa
     2701 tgccgtctga aaagatattg ttcagacggc attttgccaa caggctccgt atcggcatat
     2761 caataaagtt gcttgcgcgg catgacaagc tacggctcgt ttaattgaaa ccctcctgat
     2821 ctaaaaattc taactctatt tcccggcaaa ctatatctat cctaaaagca gtcggaaaat
     2881 catacctacc gcctatcaat tccagcgcac ctccataatc caagatttca tcatcctgct
     2941 ttaattcgcc aagttccaat gtattatcga tatttatatc ttggctcttt gtagcgtatc
     3001 catagacaaa tttcagatag ctgaactgct tgaatttaat ttttacccag tctgtataat
     3061 ttggtgtagg aatctcttga ggaaagtccg gataatccac attctggacg atgatatgga
     3121 attcatcgtt tttcaaagta tagtttaata gagtgccctc atatatatac agaaattgat
     3181 aatctttttt tggaatggta taagttgtca tattatttta ccttttcaaa aacaatgagt
     3241 ttaagatttt ttgagtccaa gttggttcta ctactcttat gaaatttcag ttgtttaaac
     3301 tcttccttta acggcccatt atattttaac cttaatccca ctcgagccag ttctccttca
     3361 tttggaattc ctcttaaata cttattgttt gatgtgccat taatcacaat ttctcccccc
     3421 tttttttgta atacgtgctg cttcaagaag ataatccatt aaccgctcaa catcccgcct
     3481 cttccaaaac cgcgcgcgcg gccaaatccg ccgtatcttt tttcaacagc aggtgcaacg
     3541 ccctgaaatc ctgttgcagc gcggcaacct tatcggggtg ttcgtaccag tccgccaacg
     3601 ccgccgccag tttttccggt acggcatcat gttgaagcag ctccggcaca gcctccttac
     3661 ccaacaggat attcggcagg ccgacatgcg gcactttgat tttgcgtttc acataagcat
     3721 aagtcagcgg cgaaatcttg tagctgatga ccatcggacg cttgcacaga gccacttcca
     3781 aagttgccgt accgctcgtt accagcaccg catccgccgc cctgcacact gtttcagact
     3841 gtctgtcgat taccgtcagc ggcaatccgg caaactccgg ccgctgcaaa acttccgcca
     3901 aacgccgctt cgtcgccgcc gttgcggcag gcagcaggaa gcgtgcggca ggatagcgtt
     3961 ccaacaacaa taatgccgtc tgaaaaaaca ccggcgccat atagtcgatt tcgctgacgc
     4021 ggctgccggg cagcagggcg aatacgggga tgccggcatc cacgcccaaa gttttccgcg
     4081 ccgtttcacg gtcgtcttcc aagggcataa gctgcgccat cggatgaccg acaaactccg
     4141 cacgtccgcc cgcatcgaga taaagctgcg gctccatcgg gaacaggcac agcacgcggt
     4201 tgacctgatg cacgattttg cccacgcgtt cccgccgcca cgcccacacc gacggactga
     4261 cataatgcac ggtcggaatg cccgtccgtt tcagcttttc cgccacaccc aaattgaaat
     4321 cgggcgcatc gataccgaca aagacatcag gtttcaacga cagcaaatcc cgtaccagcc
     4381 ccctgcgtat ccgtaaaatt tccggcagcc gcctgaccac ttcgacaaag ccgcgcaccg
     4441 ccagccgctc ctgatcataa aggctctcga aaccttccgc cttcatcagt tccccgccga
     4501 taccggtaaa ccgcgcctgc gggcaacgct cgcggatggc gcgtatcagg tgcgccccca
     4561 acaggtcgcc cgacgcttcg ccgacactga cggcaatcaa agggcttttt ttatcaacca
     4621 tattcgttct tcttcacata ctttcacgtc ccgatgccgt ctgaaggctt cagacggcat
     4681 cggggttcac attttcgtcc cgttttccaa catcaccgca aactgtgcca aatccggcgg
     4741 cagccccacc tttaacacca gcggctcgcc cgtgagcgga tggttcaggt gcagctcgga
     4801 cgcgtgcaaa aacatccgct tcaaacccaa cttctgcaaa cgacggttcg cctgataatc
     4861 gccgtagcgt tcgtcgcccg caatcggaca gccttgagat tgcaggtgga cgcggatttg
     4921 gtgcgtgcgc cccgttttca acgtcgcccg caccaaagtc aggtgcgaca gcccgacacc
     4981 gtgcaaaatg ccgtctgaaa aacggcttaa cacacggaac accgtatgcg ccgactgccc
     5041 gtccgcactg acgcgcacca tcttttcgcc ttgtgcgccg gtatatttga acaggggcag
     5101 tttgacatgg aaattgtcgt ccggcagttt gcccaccccc agcgcaaggt agattttttt
     5161 ggggtggtcg ttacggatgg cttcgtgaag tttgacgagc gcgctgcgtt tcttcgccac
     5221 catcaacaag ccgctcgtat ccttgtccaa acgatgaacc aactccaaat acttcgcctc
     5281 cggacgggcg cggcgcaact gttcgataac gccgaaactc acgccgctgc cgccgtggac
     5341 ggcaacgccg gacggtttgt cgatgaccaa aagcgcatcg tcttcgtaaa caacgtcaaa
     5401 cgcacgcgcc ggtacggcgg cacgcctttc agacggcatt tccttctccg ccacgcgcac
     5461 aggcggaatc cgcaccgtat ccccctccgc aatacggctg tcgggtttgc agcgtttctt
     5521 gttcaaccgc acctcgccgg cgcggataat gcggtggata tggctcttgg gaacaccctt
     5581 gaggattttt atcagatagt tatcaaggcg ttgacccgcc tcatgttcgg caacccctat
     5641 caagctgacc gaatctttgc ttatttcgtg cgttttcatc tataatccga acatccgttt
     5701 cagcaaaaag cgcgcccgcc gcgcctcctg aaacggttta ctttaaaacg gaattttata
     5761 ttaaaaacgc actccgcaaa gcattttccc tgcgcctgtt cccagccggc aggcgcagaa
     5821 cgtaatcaag tttgaattga ttttgccgtt tcggcgcgga agtaagacgg cagccgggcc
     5881 caagtcccaa acgcaaaccg cccaaaacac aggcatcacg ataacaagaa gaagccggcc
     5941 cccatttttc cgaccgcagc gttcggaacg cggcagacaa accgccgcat cgatctttat
     6001 agccttctgt atccgaccct tccgcacacg gttctccgga acgtgcatcc ctgcggattt
     6061 tcaactcaaa aactgattac cggttttatc cgaaaacatc ggaaaaccca gccttacgga
     6121 atgccgaacc gggcatgtgc cgtctgaacg ccgcctgccc acgaggtgat catgaaaaga
     6181 atgttattta acgcaacgca ggccgaagag ctgcgcgttg ccatcgtcga cggacaaaac
     6241 ctgctggatt tggacatcga aacgctgggc aaagaacagc gcaaaggcaa tatctacaaa
     6301 ggcatcatta cccgcatcga gccgtcgctg gaagcgtgtt tcgtggatta cggaaccgac
     6361 cgccacggct tcttgccgtt taaagaagtc tcccgctcat acttccaaga ctacgaaggc
     6421 ggacgcgcgc gcatccaaga cgtgctcaaa gaaggcatgg aagtcatcgt ccaagtcgaa
     6481 aaagacgagc gcggcaacaa aggcgccgcg ctgaccacct tcatcagcct cgccggacgc
     6541 tatctggtat tgatgccgaa caacccgcgc ggcggcggcg tatcccgccg tatcgaaggt
     6601 gaagagcgtc aagagttgaa agccgccatg gcggaactcg acattccgaa cggcatgagc
     6661 atcatcgccc gtaccgccgg catcggccgc agcgcggaag agttggaatg ggacttgaac
     6721 tacctcaaac aactctggca ggcgattgaa gaagcaggaa aagcgcatca cgacccctac
     6781 ctgctcttta tggaaagctc gctgctgatc cgagccatcc gcgactattt ccgccccgac
     6841 atcggcgaga ttttggtgga caatcaagaa gtttacgacc aagttgccga gttcatgagc
     6901 tacgtcatgc cgggcaatat aggccgtctg aaactctacg aagaccacac gccgctgttt
     6961 tcccgcttcc aaatcgaaca ccaaatcgaa agcgcgtttt cgcgcagcgt cagcctgccc
     7021 tccggcggcg cgatcgtcat cgaccatacc gaagccctcg tctccatcga cgtgaactcc
     7081 gcacgcgcca ctcgcggcgc agacattgaa gacaccgcgt tcaaaaccaa tatggaagcc
     7141 gccgaagaag tcgcccgaca aatgcgcctg cgcgacttgg gcggcttggt cgtcatcgac
     7201 ttcatcgaca tggaaaaccc caaacaccag cgcgatgtgg aaaacgtcct gcgcgacgcg
     7261 ctcaaaaaag accgcgcccg cgtgcagatg ggcaaactct cccgtttcgg acttttagaa
     7321 ttgagccgcc aacgtttgaa accggctttg ggcgaaagca gccacgtcgc ctgtccgcgc
     7381 tgcgccggca ccggcgtgat tcggggcatc gaatccaccg ccctgcacgt tttacgcatc
     7441 attcaagaag aagcgatgaa ggacaacacc ggagaagtgc gcgcacaagt gcccgtcgat
     7501 gtcgccacct tcctgctgaa cgaaaaacgc gccgagctgt ttgcgatgga agagcgtttg
     7561 gatgtcaacg tcgtcctgat tccgaacatc cacctcgaaa atccgcacta cgaaatcaac
     7621 cgcatccgca ccgacgacgt agaagaagac ggcgaaccga gctacaaacg cgtcgccgag
     7681 ccggaagaag acgaatccgc caaaccgttc ggcggcgaaa aagccaaagc cgcccgtccc
     7741 gaacccgccg tcaaaggcgt gcgccacacc agccccgccc cgactgccgc ccccgagaaa
     7801 aaaacctctt ggtgggacag cttcaaagca tggctcaaac gcattttcgg cggcagcgaa
     7861 acccaagccg cgcccgctgc cgaaacctcc gaaaaacgca gcacggcaaa ccgcagcggc
     7921 agccgcgcca acaaccgccg ccaaaacccg cgccgcagca aacgcgaagg cagcaaagta
     7981 gaagtccgcg aagtggcagg caaaactgcc ggacaggaag cgcgtgccga caaagccgaa
     8041 acgcgcaaca acggcaaccg ccgccgcaat gaacgcggcg accgtgccgc cgaacgcgcc
     8101 aacgaagcgg aaatccaaag ccgcaacgta cagcctgccg caaccgttgc agatgccgca
     8161 ccgtccgaaa ccgaaatgca aaccggcaaa cgccgccgca acggcagccg cagcgaacgc
     8221 agccaaaccg cgccggaaac cgccgccgtt gccgaaacag ccgttcagac agcggaaaac
     8281 acgccgtccg aaccgcatac cgcagaagac aaaggcagca agcccaaatc cgaacgcaac
     8341 cgccgcgaac gcgacagccg cgacgccaaa gaacgccgcg agcgcaacaa tcagcgcgac
     8401 cgccgtcaaa acggcaaaaa acgcaatatc ccgtctgccg ccaaaatcga gcagtacctg
     8461 aatattcacg acaccgccga caaagtccgt tccgccgccg cgcacgtttt cggcgaaacc
     8521 gacgcaaacg cgccgattac cgtcagcatt gccgatccgg ttgcagaaag ggatcttccg
     8581 acagcatctc ccgccgtttc aaacggcgac gcaccggttt atgatgcggc ggaaaaaatc
     8641 cgccgtgcca ccgccgccat cctgcccgaa ggcgcgacac cgaaagccga agcacaggaa
     8701 atgccgtctg aaaccgcaac ctttacggct gcggcggaac aggcacggga aaccgcacaa
     8761 accggcggac tcgtcctgat cgaaaccgac cctgccgcat tgaaggcatg ggcggcacaa
     8821 cccgaagtcc aaaccggacg cggtttgcgc cgttccgaac agcccaagcc gtctgaagtc
     8881 gcaaccgtcc ctgccgaaga aatgatccaa gtcgaaaccc ggcaaggctg aaccgacggc
     8941 ggcaaaaaga ggttctgttc cgcagaacct cttttttata tagattcgga tacctgcaat
     9001 gccgtctgaa gcctcaccat tcccatgatt accgcaacct ttcgtcattc ccgtgaaaac
     9061 gggaatctag aacctcaaac tttcaaataa cctttgaata ttgccgctgc cttaaggtct
     9121 ggattcccac ctgcgcggaa tgacggttta gaagttgccc gaaacctcaa aaaaaactga
     9181 aaccgaacaa gccggattcc cgcaacattc cgtcattccc gtgaaaacgg gaatatagaa
     9241 ccccaaactt tcagataatc tttgaatatt gccgtcgccc aaaggcctgg attcccgcct
     9301 gcgtgggaat gacggcggag ggtggacgat gccgtctgaa acttcgccat tcccatgatt
     9361 accgcaacct ttcgtcattc ccgccacctt tcgtcattcc cgtgaaaacg ggaatataga
     9421 acctcaaact ttcagataat ctttgaatat tgccgccgcc caaaggtctg gattcccgcc
     9481 tgcgcgggaa tgacggcgga gggtgaacga tgccgtctga aacttcacca ttcccatgat
     9541 taccgcaacc tttcgtcatt cccgtgaaaa cgggaatcta gaacctctaa actttcagat
     9601 aatctttgaa tattgccgtc gcccaaaggc ctggattccc gcctgcgcgg gaatgacggt
     9661 ttagaagttg cccgaaacct
//
LOCUS       DAXHPG010000067         9514 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXHPG010000067 DAXHPG010000000
VERSION     DAXHPG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.067"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            328..1146
                     /gene="opc"
                     /locus_tag="ACFM6F_001205"
                     /gene_synonym="opcA"
     CDS             328..1146
                     /gene="opc"
                     /locus_tag="ACFM6F_001205"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HFC6164618.1"
                     /translation="MKKTVFTYAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTTSGDVSLTIPKTSIREYGLRVGIKF"
     gene            1310..1954
                     /locus_tag="ACFM6F_001206"
     CDS             1310..1954
                     /locus_tag="ACFM6F_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6164619.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTRRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     FLWVILFSYAGYFFANFPIVKNNLGLVMGGIIIISILPGAVEIARAKLAVKSKR"
     gene            1973..3883
                     /locus_tag="ACFM6F_001207"
     CDS             1973..3883
                     /locus_tag="ACFM6F_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6164620.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDSGEKIGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGKIRDLLRRYH
                     RVGHELENGSGEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHTSFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENETVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSL
                     VFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            3904..4269
                     /locus_tag="ACFM6F_001208"
     CDS             3904..4269
                     /locus_tag="ACFM6F_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164621.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            4380..5855
                     /gene="trpE"
                     /locus_tag="ACFM6F_001209"
     CDS             4380..5855
                     /gene="trpE"
                     /locus_tag="ACFM6F_001209"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC6164622.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERNDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIHAAQMVQEGLDK"
     gene            5852..6398
                     /locus_tag="ACFM6F_001210"
                     /pseudo
     CDS             5852..6398
                     /locus_tag="ACFM6F_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
     gene            6395..7531
                     /locus_tag="ACFM6F_001211"
     CDS             6395..7531
                     /locus_tag="ACFM6F_001211"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC6164623.1"
                     /translation="MKNISLSPPATLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFNDQTALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            7578..8060
                     /locus_tag="ACFM6F_001212"
     CDS             7578..8060
                     /locus_tag="ACFM6F_001212"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6164624.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            8166..9221
                     /locus_tag="ACFM6F_001213"
     CDS             8166..9221
                     /locus_tag="ACFM6F_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6164625.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKATNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            9218..9415
                     /locus_tag="ACFM6F_001214"
     CDS             9218..9415
                     /locus_tag="ACFM6F_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164626.1"
                     /translation="MTAMPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGITFLNHPF"
BASE COUNT         2650 a         2691 c         2098 g         2075 t
ORIGIN      
        1 tgcgcgggaa tgacgatgaa aagattgttg tcgcttcgga taaattttta ccgtgttggg
       61 ttctagattc ccgcctgagc gggaatgacg gcggcgggtt tctgtttttc cgataaatac
      121 acacaaacta aaatttcgtc attcccataa aaaacagaaa accaagtgag aataacaatt
      181 cgttgtaaac aaataactat ttgttaattt ttattaatat atgtaaaatc ccccccccga
      241 aagcttaaga atataattgt aagcgtaacg attatttacg ttatgttacc atatccgact
      301 acaattcaaa ttttagaggt tttaactatg aaaaaaacag tttttacata tgccatgatc
      361 gccctgaccg gtactgccgc cgctgcacaa gagcttcaaa ccgctaatga gtttaccgtc
      421 cacaccgacc tctcttccat ttcttcaact cgtgctttcc tgaaagaaaa acacaaagct
      481 gccaaacaca tcggcgtgcg tgctgatatt ccttttgatg ccaaccaagg catccgcttg
      541 gaagccggtt tcgggcgcag caaaaaaaat attattaatt tggaaacaga tgagaacaag
      601 ctgggtaaga ctaaaaatgt aaaactgccc accggcgttc ctgaaaaccg tatcgatctt
      661 tacacaggct acacctacac ccaaacgtta agtgattctt taaatttccg tgtgggtgcc
      721 ggcttgggtt ttgaatcttc aaaagacagc attaaaacca ccaagcatac gcttcacagc
      781 agccgtcagt cgtggttagc caaagttcac gcggatttgc tttcccaact gggtaacggc
      841 tggtatatca acccttggtc tgaagtgaaa tttgacctca attcccgcta taaattaaac
      901 accggcgtta ccaatctcaa aaaagacatc aatcaaaaaa ccaacggctg gggctttgga
      961 ttgggtgcaa atattggtaa aaaactgggc gaatccgcca gcatcgaggc ggggccgttc
     1021 tacaaacaac gcacttacaa agaatccggc gagtttagtg taacaaccac gagtggcgac
     1081 gtatcgctca ccatcccgaa aaccagtatt cgtgaatacg gcttgcgcgt cggcataaaa
     1141 ttctgatgat ttgaaatcat cattgtcgct ttaaatgcca aaccgcaaac tattttggtt
     1201 tgcggttttt acgtgaaatg aatttgaata gccgatgccg tctgaaactt ttcagacggc
     1261 atttttccaa tcggttaaaa tacgcggttt gtttttttag gaacgcaccg tgcttgcctc
     1321 cgccatcgac ttcatcctcc atatcgacca acacctgctc gcgctgtcgg cgcaatacgg
     1381 tgtgtggatt tatgcgattc tgtttttgat tgttttttgc gaaaccggcc tgattgttac
     1441 gcccttactg cccggcgatt cgctgctgtt tgccgccggc gggattgccg cgctgggcgg
     1501 tatggatatt catttgatgg ttgcattatt aagccttgcc gccatactcg gcgacgcgct
     1561 caattttact gtcggtaagt atttcggcgg caggctgttc gccaatcccg attctaaaat
     1621 cttccgacgc gaatatctcg ataaaacccg ccgtttttac gaaaaacacg gcggcaaaac
     1681 gattatcatc gcgcgcttcg tgcccatcgt ccgcaccttc gccccctttg tcgccggcat
     1741 gggcaaaatg cattatgcaa aatttatccg ctataacatc atcggcggct tcttgtgggt
     1801 tatcctgttt tcttatgcag gctatttctt tgccaacttc cccatcgtaa aaaacaatct
     1861 gggcttggtg atgggcggca tcattattat ttccatcctg ccgggtgcag ttgaaattgc
     1921 ccgcgccaaa cttgcggtaa aatccaaacg gtaactgttg agaatttgta tcatgaatat
     1981 attatccgta gaaaacgctt cctttgccgt cggccatgtc gccctgctcg acaaaacgtc
     2041 cttccaactc gacagcggcg aaaaaatcgg cttaatcgga cgcaacggtg cgggtaagtc
     2101 ttcgttttta aaaatcctcg ccggtttgca aaagctcgac gacgggcaga ttattgttca
     2161 aaacaacctc aaaatcgttt atgtaccgca ggaatccttt tttgataagg acgcaaccgt
     2221 atttgatacc gttgccgaag gtttgggtaa aattcgcgat ttattgcgcc gttaccaccg
     2281 cgtcggtcat gagttggaaa acggttcggg tgaggctttg ttgaaagaac tcaacgaatt
     2341 acaacttgaa atcgaagcga aggacggctg gaagctggat gcggcagtca agcagacttt
     2401 gggcgaactc ggtttgccgg aaaacgaaaa aatcggcaac ctttccggcg gtcagaaaaa
     2461 gcgtgtcgcc ttggcgcagg cttgggtgca gaagcccgac gtattgctgc tggacgaacc
     2521 gaccaaccat ttggatattg acgcgattat ctggttggaa aacctgctca aggcgtttga
     2581 aggcagcttg gtcgtgatta cccacgaccg ccgttttttg gataatatcg ctacgcggat
     2641 tgttgaactt gaccgcggca ttctacgttc ctatcccggc tcgttctcta aatacagtga
     2701 gaaaaaagcg caagagttgg cagtcgaggc ggaacataac cgcttgtttg acaaatttca
     2761 tgctcaggaa gaagcgtgga tacgcaaagg catcgaagcg cgacgtaccc gtaacgaagg
     2821 acgcgtgcgc cgtttggaag agctgcgccg ccaacgtgca gaacgccgca acgtgcaggg
     2881 acaggtcaac tttaagctcg atagcggtga gaaaagcggc aaaatcatcg ccgaattgga
     2941 acacacttcg tttgcctatg gcggcaaagt cattatggac aaattctccg ccatcttgca
     3001 gcgtggcgac aaaatcggct taatcggccc aaacggtatc ggcaaaacca ccttcctcaa
     3061 gctgattttg ggcgaattgc agccgaccta cggcagaatc cgtatcggca gcaagcagga
     3121 agtcgcctat ttcgaccagt tccgcagcgc gttgaatgaa aacgaaaccg tgttttacac
     3181 gctcggacag ggcaatgatt acgttgaagt cggcggtaag aaaaagcatg tgatgagcta
     3241 tttagaagat ttcctgttcc cccctgcccg cgcgcaaagt cctgtttctt cactctccgg
     3301 cggcgaacgc aaccgccttt tgctggcaaa actctttacc cgtcccgcca atattttggt
     3361 cttggacgaa ccgaccaatg acttggacat cgacacccaa gagctgctcg aagacctgtt
     3421 gcgcgattat caaggcacgg tattccttgt ctcgcacgac cgtatgttcc tcgataacgt
     3481 gattacccaa agccttgttt ttgaaggaaa tggaaggtta aaagaatata tcggcggcta
     3541 tcaggactac atcgacgcaa aatcacgcga agcgaagatt cagacggaat ccgcacccaa
     3601 aacagccgat gccgaaccgg tgaaagaaaa acccaaagcc aaccgcacgg tcaaactgtc
     3661 ctacaaagaa cagcgagaac tcgatgccct gcccgacgaa atcgccgcct tggaagccga
     3721 acaggcagaa atcaacgcgc agctttccga tcccgaaatc ttcaaagatt acgaaaaagc
     3781 cggcgcactg caaagccgcg ccgaagaaat cgaaatgctg cttttggaaa agctggaacg
     3841 ctgggagctg ctggaaacca aacagaacgg caatgccgtc tgaaatcaaa caggaagaaa
     3901 aaaatgttca aacgacctga agaaatcatc gtcctgatac ttgccgtcct ctggattgcc
     3961 ggtacgtatt ttctcgccgc cctgtttgga gcggacgctt ataccgtgtt aaaaatcact
     4021 gcccttaccc tgctttggtc tgccgcatca ttcctccttt ggcaaaaaaa cctcagccgg
     4081 catatctggc cgctgctgct cggctgcctg actgcctgtt ggtggccgta tctgaatcaa
     4141 tcggtcgcac ccggttcttt accttggtat gcatcatgga cgttcaaaac catcttagcc
     4201 ctgattcccg taaccgcagg ttatctgttt aaatggaaac aataccggca gcgcaaaaca
     4261 aagccgtaag cccgtctttt tcagacggca tctccggtca gactgtcaac aattccgata
     4321 aaatgctata ctcgcgcccg ttgccgcgcc gtccgaaacc gccctttggg aaagcagaaa
     4381 tgatcagcaa acaagaatac caagcccaag ccgcccaagg ctacaaccgc atcccgctcg
     4441 tgcaagaact ccttgccgat ttggatacgc cgctttccct ctatctcaaa ctcgccaacc
     4501 gcccctatac ctacctgctc gaatccgttg tcggtggcga acgtttcggc cgctattctt
     4561 ttatcggcct gccttgcagc cactatctca aagccagcgg caaacacgtc gatgtttatc
     4621 aaaacggcga aattgtcgag caacacgacg gcaaccccct gccctttatc gaagccttcc
     4681 acaaccgctt caaaacaccc gaaatcccaa gcctgccgcg ctttaccggc ggactggtcg
     4741 gctacttcgg ttacgaaacc atctacaatt ttgaacactt cgcccaccgc ctgaaaaaca
     4801 ccaccaaagc cgacccgctc ggcacgcccg acatcttgct gatgctgtcg caagaattgg
     4861 cggtgatcga caatttgagc ggcaaaatcc atctcgtcgt ttacgccgat ccgtcgcagc
     4921 ccgacggcta cgaacgcgcc cgcgaacgcc tcgaagacat ccgcacccag ttgcgccaaa
     4981 gctgcgccat cccgctctcg ctcggcagca aacacaccga agccgtcagc gaattcggcg
     5041 aagagccgtt caaagcctgc gttaacaaaa tcaaagacta catcttcgca ggcgactgca
     5101 tgcaggttgt ccccagccag cgcatgagca tggaattcac cgacagcccg ctcgccctct
     5161 accgcgccct gcgtacgctc aacccttcgc cttatctctt ttactacgat ttcggcgatt
     5221 tccacatcgt cggctcctcg cccgaaatcc tcgtccgacg cgaacgcaac gacgttatcg
     5281 tccgccccat cgccggcacg cgcctgcgcg gcaaaacccc cgccgaagac cttgccaacg
     5341 aacaagattt gttaagcgat gccaaagaaa tcgccgaaca cgtcatgttg attgacttag
     5401 gacgcaacga cgtcggccgc atcagtaaaa ccggcgaagt caaagttacc gacaaaatgg
     5461 tgattgaaaa atactcccat gtaatgcaca tcgtttccaa cgtcgaaggc cgtctgaaag
     5521 acggcatgac caacatggac atcctcgccg ctaccttccc tgccggcaca ctctccggcg
     5581 cacccaaagt ccgcgccatg gaaatcatcg aagaagtcga accgagcaaa cgcggcatct
     5641 acggcggtgc cgtcggcgta tggggtttca acaacgacat ggatttggca atcgccatcc
     5701 gcaccgccgt agtaaaaaac aacacgctat acgtccaaag cggcgcgggc gtggttgccg
     5761 actccgatcc ggcctccgaa tggcaggaaa cacaaaacaa agcccgagcc gtcatacacg
     5821 ccgcgcaaat ggtacaggaa gggttggata aatgacttat gaaaaacttt atgagcttcg
     5881 ccaaacactc agaccgacta ccaaagacgg tctctataca gataatgaaa aaaatcgaga
     5941 aatcctaacg gttagatgga gcggcaacac agaaaatcct aaaaatttct cagctgtcgc
     6001 tataggaatt aatccaagta aagcaaatga tgaaagaagc gacaaaaccc ttacccaact
     6061 tgcacgtttc ttggatatgt acggttttac caacttcaaa atgttaaata ttttttccag
     6121 ttactctaca cagcaaacag gaattaggac aaacacccaa accgattttt ccaaatttaa
     6181 aggatgtttg gaagatgctg atatgattat tcttgcatgg ggtacagacc gcggtgctta
     6241 taaggatgaa aaaaaacaga atactggaat ttctaaaagc agaaaaattt atggaaaaag
     6301 tattctgtat ttctgaaact ggtaattcca gcgatacaag acatccgtca agaattagtt
     6361 atagctatca acttgtacaa tttgaagagt cagcatgaaa aacatatctc tttctccgcc
     6421 cgccacgctc ggaatcctcg gcggcggaca attaggcaga atgtttaccg ttgccgccaa
     6481 aaccatgggc tacaaagtaa ccgtactcga ccccgatccg aacgctccgg cggcggaatt
     6541 tgccgaccgc catttgtgcg cgccgtttaa cgaccagacc gcgttggacg agttggcaaa
     6601 atgcgcggcg gttacgaccg aatttgaaaa cgtcaatgcc gacgcgatgc gttttctggc
     6661 aaaacatacc aacgtttccc ccagcggcga ctgcgttgcc atcgcgcaaa accgcattca
     6721 ggaaaaggca tggatacgca aagcaggtct gcaaaccgcg ccgtatcaag cagtttgcaa
     6781 agccgaagac atcactgaag caagcgcgca attcctgccc ggtattctga aaacggccac
     6841 tttgggctac gacggcaaag gtcaaatccg cgtcaaaacg gtggacgaac tcaaagccgc
     6901 gtttgccgaa cacggcggcg tggattgcgt tttggaaaaa atggtggatt tgcgcggcga
     6961 aatttccgtt atcgtatgcc gtctgaacaa tgacaacgtg caaactttcg atcctgccga
     7021 aaacattcac gaaaacggta tcctcgccta ctccatcgtc ccagcccgac tgagtgccga
     7081 cattcagcaa caggcgcgac aaatggcgca gcgtttggcc gatgaattga actacgtcgg
     7141 cgtattggcg gtagaaatgt ttgttgtcgg cgacacgcat gaattggtcg tcaacgaaat
     7201 cgcgccgcgt ccgcacaatt ccggccacca taccgtcgac gcctgcgcgg cagaccaatt
     7261 ccagcaacag gtccgcctga tgtgcaacct gccacctgct gacaccaaat tgctgagttc
     7321 ttgctgtatg gcgaatattt tgggcgacgt ttggcaggaa gacggcggcg aaccggattg
     7381 gtttcccctg caaagccggc cggacgcgca cctgcacctt tacggcaaaa aaaccgcgca
     7441 caaagggcgg aaaatgggac actttaccgt tttaaccacc gattcggaca ccgcatttca
     7501 agaagcaaaa aaactgcatc agtccctata atccgaccaa cggcacagat gccgtctgaa
     7561 gccgccgaca tactgccatg aacctgctga cactgttacg ccccgccacc gtgcaagact
     7621 gcaaagacat cttcaaagtc cacctgcact ccgtccaata cacctgcatc ctcagctaca
     7681 acgaacacgc cctaaaagta tgggaaggct tgttaaacac agaaagctac ctgccgacca
     7741 tttccgatcc cgacaaagca ctttgggttg ccgaatacaa aggcaacata cagggatttt
     7801 tccaaatcga ctgccaagaa gcacaactcg atgcacttta cgtccacccc ctgttccaca
     7861 acctcggact gggcactgcc ctgcttcatc aggcggaaac catcgcccac aaatctggtt
     7921 tgagctttct gaaactctac gcatcgctca actccgtccc gttttacctt ctcaaccgct
     7981 acgaatcgct cggttcggca gtattgcagc ttgaccccag catcaaaatc aaatgcgaac
     8041 tgatgcggaa acacctctga tccgtcctta taaccaaaac tgataatctt caaatcaaac
     8101 attatttcca caagcgcaga cagccgtata aagttgcccc atctaccaac cgacaggaca
     8161 agcatatgaa aacctacgca ccggcactct ataccgcagc cctgctcacc gcctgcagcc
     8221 ccgcagccga ttcaaaccat ccgtccggac aaaatgcccc ggccaatacc gaatccgacg
     8281 gaaaaaacat taccctgctc aacgcctcat acgatgtggc acgggatttt tacaaagaat
     8341 acaacccctt atttatcaaa acataccaat ccgaacaccc cggcacatcc gtcagcatcc
     8401 aacagtccca cggcggctcc agcaaacagg cattatccgt agccaacggc cttcaagccg
     8461 atgtcgtaac catgaaccaa tcctccgaca tcgacctgct cgaaaaaaaa ggactggtag
     8521 aaaaaggctg gcaacaagcc ctccccgacc acgccgcgcc ctacaccagc actatggttt
     8581 tccttgtccg aaaaaacaac cccaaacaga tccgcgattg gaacgacctt gccaaagacg
     8641 gcgttaacat cgtcatcgcc aatcccaaaa cctcgggcaa cggacgctac gccttcctcg
     8701 gcgcatacgg ttacggtctg aaagccacca acggcaacga acaggaagcc caaaaactcg
     8761 tcgcatccat cctcaaaaac acccccgttt ttgaaaacgg cggacgcgcc gccaccacca
     8821 ccttcacaca acgcaacatc ggcgacgtac tcattacttt tgaaaacgaa gccaactacg
     8881 tcagcaaaaa actgacccaa ggacagtttg aaatcgtcta tccgagctac accatttccg
     8941 ccgaaagccc cgtcgccgtc gtcaacagcg tcgtcgcgaa aaaaggcacg caaaaaaccg
     9001 cccgcgccta tctcgaatac ctttggagcg aaccggcgca agaactcgcc gcctcactct
     9061 acctgcgccc ccgcaacccc gaagtattgg caagacacaa agccgacttc ccagacttag
     9121 acaccttctc tcccgaagaa aaattcggcg gatgggacaa catcatgaaa acctacttcg
     9181 ctgacggcgg catattcgac cgcctgaccg cacaaaaatg accgctatgc cgtctgaacc
     9241 gcctttcaga cggcattcca cacatcaaaa cagaaaaacc gcgcccaaaa tcatcatcac
     9301 aagacacaac ggcaaaccgg atacaaaacc caacaccgcc gccaaaatgc cgtctgaagc
     9361 cgtcggcgca accatcctta tctggggcat aactttcctt aaccatccct tttgacagcc
     9421 aaataaacag tgcttttgcc tgtcagaaac aaaaccaata ttttgcagtt cgtatcggaa
     9481 catttatagt ggattaacaa aaaccagtac agcg
//
LOCUS       DAXHPG010000068         9504 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXHPG010000068 DAXHPG010000000
VERSION     DAXHPG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.068"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(1066..1734)
                     /locus_tag="ACFM6F_001215"
     CDS             complement(1066..1734)
                     /locus_tag="ACFM6F_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6164627.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(1731..2399)
                     /gene="hda"
                     /locus_tag="ACFM6F_001216"
     CDS             complement(1731..2399)
                     /gene="hda"
                     /locus_tag="ACFM6F_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC6164628.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(2504..3094)
                     /locus_tag="ACFM6F_001217"
                     /pseudo
     CDS             complement(2504..3094)
                     /locus_tag="ACFM6F_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(3304..4021)
                     /locus_tag="ACFM6F_001218"
                     /pseudo
     CDS             complement(3304..4021)
                     /locus_tag="ACFM6F_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173203.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
     gene            4243..4446
                     /locus_tag="ACFM6F_001219"
     CDS             4243..4446
                     /locus_tag="ACFM6F_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC6164629.1"
                     /translation="MKKKQESMTDWHRADIVARLKKAGWSVRALSIEANLAPNTLGKA
                     LDAPYLKGERIIAAAIGVPAEEI"
     gene            4568..6556
                     /locus_tag="ACFM6F_001220"
     CDS             4568..6556
                     /locus_tag="ACFM6F_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFC6164630.1"
                     /translation="MKISASDIAKLGIPSLPTDRQGIEYHAKKNNWQHCFEQKGRGRP
                     KKLYEIASLPAEIRAAIMKRQSDELAEKMPKMLPQVRPGTAMSAQALAEAAKLLNEKQ
                     RSVADARCAVVAAVLGIKYEYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRS
                     AKVGERTLDGWISAYLKAENAIERLVALAPKTTKAVKPIESYGWLPMFMRFHNIPSAP
                     KLAHSYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYV
                     KRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAES
                     CVAVSDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQ
                     GRGIIERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQ
                     KYLKKLPSWSRFIADVVKCIDDYNNRPHGELPRHPDGGHYSPKAYREIRLEQDGIAPD
                     MLSAEELATMFMPQEVRKVQRGWLDLFSNSYFSTELAEYHKDEVRVSYDLSDASAVNV
                     FDMDGKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQ
                     AAAWDELGHLGGNVIEAEYAVLPKTGTDDGIFLFEADM"
     gene            complement(6623..6880)
                     /locus_tag="ACFM6F_001221"
     CDS             complement(6623..6880)
                     /locus_tag="ACFM6F_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC6164631.1"
                     /translation="MKEFPDLEKRLIMLEVAVQDLEERSLTDSFVLAWLLQRITRQEP
                     TSIEQVRSFLQAQAKTFEPDSVQREHLESLLEIVESAQGLA"
     gene            6926..7684
                     /locus_tag="ACFM6F_001222"
                     /pseudo
     CDS             6926..7684
                     /locus_tag="ACFM6F_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            7658..8098
                     /locus_tag="ACFM6F_001223"
     CDS             7658..8098
                     /locus_tag="ACFM6F_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFC6164632.1"
                     /translation="MAKAALPDAVKHLLPDSVQALITVIGFNETLELVRLMGGTTYPL
                     RQGYTKNSQSRVAYLEEIIGSEAAGRLVEAMAPCNLFIPRCETALYELRNRKIRSQFD
                     RQTAGGTPAYEAVNDLALAHRLSDRHVWRILKQADKEAEQENLF"
     gene            8100..8621
                     /locus_tag="ACFM6F_001224"
     CDS             8100..8621
                     /locus_tag="ACFM6F_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164633.1"
                     /translation="MECHADVWHFILEKNMKKFYFVLLALGLAACGQEQSQKADAEQY
                     FFANKYQFADEKQAFYFERAARFRVLQQGLGGDFERFLKGEIPNQENLAKYRENITQA
                     VAYYADTNGDDDPYRVCKQAAQDAEILMKSMVTSGGGGTTDLDKESYQNYRKSMQECR
                     KTITEAEANLPKK"
     gene            8764..9309
                     /locus_tag="ACFM6F_001225"
     CDS             8764..9309
                     /locus_tag="ACFM6F_001225"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6164634.1"
                     /translation="MSKIICLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMPLRDAVKLIRGSDVAIEFHTNAAANKTATGIEALSTPKNKRWCQ
                     VLGKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGATKV"
     gene            9306..>9504
                     /locus_tag="ACFM6F_001226"
     CDS             9306..>9504
                     /locus_tag="ACFM6F_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164635.1"
                     /translation="MKKSLIALCVAHCAKLKNDFGVPPLPEIKITPSPVRVGSLKQHP
                     SLRLGKSGVAAAKRAARKRKNR"
BASE COUNT         2481 a         1985 c         2638 g         2400 t
ORIGIN      
        1 tcgccttgtc ctgatttttg ttaatccact atacgtccta gattcccact ttcgtgggaa
       61 tgacgggatg tgggtttttg tgcggatttg aaccggtaag ggtggtgtgg gattggtggt
      121 ttgcttagga tcttttggat tgtattttgt atatacattt acttgttgat aaaagataaa
      181 ataaaattag aaactaaaag tgagaaaaaa ttaataataa tagggatgta taaatgtaaa
      241 ggctccgttt catagctaag gttatctgaa tatatggaaa aaaagtaaaa gtccataaaa
      301 ctaaaatata tagataatgc taataataat agaattatca ctttcctaga gtaaggataa
      361 tattttttta tcgtaaaaaa atataaaata ggataaataa ttgccatata gatagatttt
      421 gtaacaaaat taaaaataaa taataaaaat aatgtaataa agtatatttt ttggctatga
      481 aataaaattg tacataattg aacgagcaga tcaaaaaatg aactacatat aacaataaat
      541 aataacgtat ttaccataat aaatttaata ggtctcatta tcatatttaa taaccacttc
      601 atagtatagt ggattaaatt taaaccagta cagcgttgcc tcgccttgcc gtactatctg
      661 tactgtctgc ggctttgtcg ccttgtcctg atttttgtta atccactata aatgcagagt
      721 gggtggaaac actcacttta tggtttgcta cgctctgctc aattagcaac ccgataaccc
      781 aatatggata atagggtaat taatccaatc taatttgtca gtatccgtta atttattgca
      841 aaataaagta ttgaattatg tcgggtgcaa atgacgaaat ataagtttcc gtgcggacgg
      901 attaagattc ccactttcgt gggaatgacg gtggaaagat tgttgttttt cccgatgaat
      961 tcctgtgttt tttgtttttc cggataaatt cctgtggctt tgagtttttt ggatttcagc
     1021 ctcaatgccg tctgaacgcc gaatcgggct tcagacggca ttgcgtcatt tgaaattcaa
     1081 aaccggccag cctttttctt tggcttcttt ttccagctcg gcatcggggt tgacggcgac
     1141 gggttcgtcg acgaggcgca gcagcggcag gtcgtttttg gagtcgctgt aaaaataggt
     1201 tttgccgtag ctttcgagcg tttcgccgcg ttcggcgagc cattggttca ggcgggtgat
     1261 tttaccttct ttgaggctgg gcgtgccgat gtaattgccg gtgtagcggc cgtcggaacc
     1321 ggattcgagt tgtgtgccga tgatgttggt aatgccgaaa aggcggcaga cgggggtgat
     1381 gatgaactcg ttggtcgatg agatgacgag ggtttcgtcg cctgccattt ggtggctctg
     1441 caccagcata cgctgcatag gcgagatgtg ggggatgatg tattccgcca taaattcgcg
     1501 gtgaaactct gccagctctt ctttgctgta acgagcgagc ggggcaaggt ggaatttgag
     1561 gaatgcgtcg atgtcgaggc aaccgttttg gtagtcgcgg tagaattttt cgttttgcgc
     1621 ttcggtttcg gcagcgtcaa ccaagccttt tttgatgagg tattgcggcc aggcgtggtc
     1681 ggaatcggtg ttgatgaggg tgttgtcgag gtcgaagatg gcgaggtttt tcattgggtt
     1741 tcctgttgtt tcaaaagctg gcgcaaaagc ggcagggtga tgcgtttgcc cattgtgacg
     1801 gcgtagttgt ccagcgtgtc gagcatcatc atcaggctgt ccatatcgcg ccgccagtgt
     1861 ttgagcaggt attcgaaaat ttcggaatcg acggttactt ggcgtgccgc cgccatactg
     1921 gcgagcgcgt cgattttttc ttggtcggtt aagggtttga cttcgtaaac gaggcagtac
     1981 gccatacgcg tccgcaaatc ttcgcggatg acaagctgct ggggcgtgta ttccgaaccg
     2041 agcagcaaaa agcctttgcc gctgttgcgg aagcggttga agatggaaaa aagcagggct
     2101 tgttcttcgt tgcccagttt ttcgacttga tcgacggcga ggtattccgc ctcgaacgcg
     2161 gcatcggtca gcggcatgga ggcggcatcg atataggcgg cgtttttgcc ggcttcgagc
     2221 gcctgtgcga cccacgcctg caaaagatgg cttttgcccg cgccttcttc gccccaaaca
     2281 tagataaact gtccgtgttt gtgtcggagg acatagacca gttccgcgtt ttccgtgccg
     2341 aggaatttgt cgaaactcgg atagtcgtgt gcggcaaagt cgaaaataag ctggttcacg
     2401 gttcggcatt ccgaggggtg gtaaacgggt ttattgtacg ttgttttcgc gcgcctttcc
     2461 aatttgaacg atgccgtctg aaaacggctt cagacggcat cgttcagccg caggcagcgt
     2521 tgccgacatc gaggtgcata ttgtggaacg cgttgagcgt gctgcggtgg ccgatgctga
     2581 tgatgatgct gtcgggcagt ttttgtttca gtgcgcggta gagcagggcc tcggtcggtt
     2641 cgtccaaagc ggcggtggct tcgtcgagca ggacgatttt gggcttggaa agcagggcgc
     2701 ggacgaaggc gacgcgttgc agttcgcccg gggagagttt gtgttgccag tcgtcggttt
     2761 tatctaattt atcaaccaga taacccaagc ggcaggtgtt catggcttcg gctaactcgg
     2821 gatgctgctt gtcaatgtcg gggtaacaaa ccgcgtcgcg caggctgccc tgtgccgtgt
     2881 acgggcgttg cggcagggag aggatgtctt gatgcggcgg acggctgact ttgccgctgc
     2941 tgccgaacgg ccaaagcccc gccagcgcgc gcaacagcga ggttttgccg caaccgctcg
     3001 ggccgcgtat cagcagggaa tcgccgtttt tgaggtttat gttgatgccg ctcaacagga
     3061 tttcgccgtt gtggcagaac agagcgacgt tttcctgtgg gggactgtta gtttttgcac
     3121 aaggaacaaa tagagtaaaa aaacgctgaa atcttcggaa gacgtggatt tcggcgtttt
     3181 tttgtatccg gaaaagttac gccagctttt tcacaaaacc gcgccggaat gcgcggtttt
     3241 ctgtttaaag ctgacgagat tagggaattt ttaaaactgt tttaagaggt ttttaaaatg
     3301 gatttaatca atactccggc cataccactc aacacggcct atgatggcga tgtcgtcttg
     3361 ggcattgctc aaatctattt caaacggtgc gtaacgtgga ttttcagacg ttacaagcag
     3421 tttgcccggt atacgttgca cacgtttgac aaagaggtca ttgcctatac gcaagacata
     3481 taggccgtca cgcgggtcag tttcggcgtg gttgatgaga atggaatccc catgattgag
     3541 cacgccctcc attgaatcgc ctttaacggt aattacagac agtttttccg gctgtttggt
     3601 cacatagttg tcaatccaat atttccggaa agccaagcag aataaaggtt cttcgccgaa
     3661 gactggtgcg ccataccctg ctgctgcggc tacgttgtag cgcggcacga atacaaactc
     3721 ggacaggtcg acgattgccc atagtgtcgg tgattccatc agaatttcta cttacagaga
     3781 atgctccggc gttttccggc ctggctttat cgagatacgg caagcctttt ccggtcagca
     3841 gccagtttaa atcacaaccg gtaacttctt gaatttttac taggtaatcg gctgttggga
     3901 tagctccctc tttccaaact ctattaaatc cagaagccga catttctatt ttgttataga
     3961 tgtcagatgg cttagcccca tgaggccaaa gaaatttgag cctatctaaa aaagtatcca
     4021 tagtaatcct aatttaactc atttaagcaa aacattaagc aaaaaaagaa actcttttgc
     4081 ttaaataaga ttactcaaat aatcaatatt ttgtaaaaat aattacgttt ttgagaaaat
     4141 attttagcaa aagagtttca tgaagctgtt ttgctaaatg taattcactc atttgctaaa
     4201 tgacggcggt taataaacct acttaattaa ggaattgcga gaatgaaaaa aaagcaagaa
     4261 agcatgactg attggcatcg tgctgacatt gtggctcgtc ttaaaaaggc aggctggtct
     4321 gtgagagcgt tatctatcga ggctaattta gcaccaaata cattagggaa agctttagat
     4381 gctccttatc tgaaaggcga aagaatcatt gcagcagcga ttggagtacc cgcagaagaa
     4441 atctagccat ctcgttttga gaaacgaaac cataagccaa ccttcccaag atctataaat
     4501 agataactgt tttgctaaat agttccaaaa gagtaccgca tttaagcaaa aatagaaagc
     4561 ggaaaaaatg aaaatatctg catctgatat tgcgaaatta ggaattccga gcctaccaac
     4621 tgatagacaa gggattgaat accatgccaa gaaaaataat tggcaacact gttttgagca
     4681 aaaaggaaga ggccgtccta aaaaactgta tgaaatcgct tccctccctg ccgaaatccg
     4741 agcagccatc atgaaacggc agtcggacga gctggcggag aagatgccga aaatgctgcc
     4801 ccaagtcaga ccggggacgg cgatgtcggc tcaggcactg gctgaagcgg ccaagctgtt
     4861 gaacgagaaa caacggtcgg tggcggatgc gcgatgtgcg gtggtggcgg cggtattggg
     4921 gattaaatac gaatacgatt gctctgccaa ggctgcggtg gctcagtttt tgggcttgct
     4981 ggcagaaggt aaattggacg cggtcacgct tgggaacttg gaaaaggcca atgaccgcag
     5041 ccggtcggcg aaggttggcg aacgtacttt agacggctgg atttctgctt atttgaaagc
     5101 ggaaaacgcg atagagcggt tggttgcttt ggctccgaag acgacgaagg cggtcaagcc
     5161 gattgagagc tacggttggt tgccgatgtt tatgcggttt cacaacatcc cttcggctcc
     5221 gaaactggcg cacagctacc gccggtttgt gcagtgggcg gaagcggaaa atatgccggt
     5281 caatgatgtg cctaatttga gtatggtgcg gcgcgtttgg gacaagctcc cgctaattat
     5341 gcaggagcgc ggcaggaaaa cgggggcggc ttataaatcg ctgctgcctt atgtgaaacg
     5401 tgattggggg gctttgaagc cgaacgatgt ttggatcggc gacggccaca gctttaaggc
     5461 gaaggtggca caccctgtac acggcaggcc gtttaagccg gaagtgacgg tgattattga
     5521 tggttgtacg cggtttgtgg tcgggttttc ggtttcgttg gctgaaagtt gtgtggcggt
     5581 atcggatgct ctgcgtatcg gggtcaagca ttttggtttg ccgattatct attactcgga
     5641 taacggcggc ggccaaaccg gcaagacgat agaccatgaa atcacgggta ttacgtcccg
     5701 actgggtatc cgccatgaaa cgggtatcgc gggcaacccg caaggtcgag gcatcatcga
     5761 gcgatggtgg aaagacaatc tgattgagat ggcgcgccag tatgagacgt ttgcgggcgc
     5821 aggaatggac agtagcacga agaatctgat gtaccgcaag atggaaagtg cgtttaacgc
     5881 tttggaaaaa ggcaaggatt taacggagga acagcaaaaa tatttgaaaa aactgccgag
     5941 ctggtcgcgt tttatcgcgg atgtggtcaa gtgtatcgat gattacaaca accgtccgca
     6001 cggcgagctg ccccgacatc cggacggcgg gcattattcg ccgaaggctt atcgggaaat
     6061 aaggctggaa caggacggta tcgcgccgga tatgttgtcg gcggaagagc tggcgacgat
     6121 gtttatgccg caggaagtgc gaaaggtaca gcgcggttgg ctggatttgt tcagcaactc
     6181 ttatttctca accgagctgg cggagtatca caaagacgag gtacgggtca gctacgattt
     6241 gagcgatgcg tcggcggtca atgtgtttga tatggacggc aagtttatta ctaaggcgca
     6301 ggccaacggc aatacccgcg aggctttccc gacagcgcgt atcgaccaac tggcggaaaa
     6361 acgccgaaaa ggcaaaatca agcgggcgga aaatgcaatc aagctcgcga atgcggaagt
     6421 caatccggca ctggaacagg cggcagcttg ggacgagctg ggacatttgg gcggaaacgt
     6481 catcgaggcg gagtatgcgg tattgccgaa aacgggcacg gacgacggga ttttcttgtt
     6541 tgaggcggat atgtaaagga aaacatgatg gaaaaacaga aaaatgcggc gttttcggcc
     6601 gagcttgttg aaaaattgaa actcaagcga gcccttgggc ggattcaacg atttcaagca
     6661 aagattcaag gtgttcccgc tgaacggaat caggctcaaa cgtttttgcc tgcgcttgaa
     6721 ggaaactgcg aacctgctca atcgaagtcg gctcttgacg ggtaatccgc tggagcagcc
     6781 aggcaagtac gaaggaatcg gtaagcgacc tttcttccaa gtcttgaacg gcgacttcca
     6841 gcatgatcag gcgtttttcc aaatcgggaa actctttcat ttcagacggc ctttaaaggt
     6901 tgtttaaaac tcaaggatat taaaaatgaa acaaattaat caagcattac aacaaaaact
     6961 ggttgaattt aaagaaaaat cgggtatgaa ccaaacccaa ctggcacgcg gtatcgatac
     7021 ttcgccggca tccatcagta tgtatctgaa cggcacttat gcggaaaaag gcggcaatta
     7081 tgaaaccatc gagacgaaaa tcgaggcgtt tttggagatg cagaaaagta aggcgcgacg
     7141 cgaagaactg gtgttgggtt ttgtagcgac taagacgacc cgccgaatcg cggaagtgat
     7201 gcgcaatgcg cacgaaggcg gcgaaacagt ggtgatctac ggtcaggcgg gattgggaaa
     7261 gactcaggcg gtcaaaaact actgcgagaa aaacccagcg gccatcttga ttgaggctaa
     7321 tccgagcttt acggctttgg tcttgatgcg caagttggcg acggcggcga aggtatcggc
     7381 gatgggcagc ctgaatgatt tgtttgagtc tgtatctgac cgcctgcgcg attcgggccg
     7441 tctgattgtg gtcgatgaag cggaaaacct gcccttgcgc gcccttgaga ttatccgccg
     7501 actgcatgat gaaacgggct gcggcttggt gttgagcggt atgccccgac tggtggccaa
     7561 cctgcgcggt aagcatggcg agctggtgca actttatagc cgcgtgtctg ttgcgctgaa
     7621 tttgggcgaa tctttgccgg atgacgagct ctttgagatt gcgaaagcgg ctttgcctga
     7681 tgcggtcaag catctgctcc ctgatagtgt acaagcgttg attacggtca tcgggtttaa
     7741 tgaaacgctg gagctggtgc gcctgatggg cggtacgact tatcctttgc ggcagggtta
     7801 tacgaaaaac agtcaatccc gtgttgcata cttggaagag attatcggca gtgaggcggc
     7861 cggtcggctg gtggaggcaa tggctccgtg caatctgttt ataccccgtt gcgagacggc
     7921 cttgtatgag ttgcgaaacc gtaaaatccg cagtcagttt gaccggcaga cggcaggcgg
     7981 tacccctgct tatgaggccg ttaacgattt ggccttggca caccgcctaa gcgaccgcca
     8041 tgtgtggcga attttaaagc aggcggataa ggaagcggag caggagaatt tgttttagaa
     8101 tggaatgcca tgcagatgta tggcatttta ttttggagaa aaatatgaaa aagttttatt
     8161 ttgtgctgct ggcgttgggt ttggcagcgt gtgggcaaga acaatcgcag aaagctgatg
     8221 cggagcagta tttttttgcc aataaatatc aatttgcaga tgagaaacag gctttttatt
     8281 ttgaacgcgc cgcccgtttc cgtgtattgc aacaaggcct tggcggggat tttgagaggt
     8341 ttttaaaagg agaaatacct aatcaagaaa atcttgcaaa gtatcgtgaa aatattactc
     8401 aagcagtcgc ttattatgcg gacacgaatg gagatgatga cccataccgc gtctgcaaac
     8461 aggctgcgca agatgcagaa atcctgatga agagtatggt aacaagcggt ggaggcggta
     8521 caactgattt agataaggaa agttatcaaa attaccgaaa atcaatgcaa gaatgccgta
     8581 aaacaataac ggaagctgaa gccaatttgc cgaaaaaata aaataaacga ttctaaggcc
     8641 gtctgaacaa caggcggctt ttttgttgcc tactgacact gtttcgcccg ctgcaaaagc
     8701 catgccgttt gaaaatgtaa gcctctgaaa gtgcatttta atctgatttt gagggaggct
     8761 ttaatgagca aaattatttg tctgactgcc ggacacagta acaccgaccc gggcgcagtc
     8821 aacggcagcg accgtgaggc ggacttggcg caggatatgc gcaacattgt ggcttcaatc
     8881 ctgcgtaacg attacggcct gaccgtacgc accgacggcg aaggcaaagg caatatgccc
     8941 ttgcgcgatg cggtcaagct gattcgcggc tcggatgtgg cgattgagtt ccacaccaat
     9001 gcggcggcga acaaaacggc gacaggcatc gaagccttgt ccacgccgaa aaataaacgc
     9061 tggtgtcagg tgctgggcaa agccgttgcc aagaaaaccg gctggaaact gcgcggcgaa
     9121 gacggcttta agccggataa cgcagggcaa cattcgcgcc tggcttatgc gcaggcaggc
     9181 ggcattgtgt ttgagccgtt ttttatcagt aacgacactg atttggcctt gtttaagacg
     9241 accaaatggg gcatctgccg cgcgattgcg gacgcgatcg cgatggaatt gggagcgacg
     9301 aaggtatgaa aaagtctttg attgctttat gtgttgccca ttgtgcaaag ttgaaaaacg
     9361 attttggcgt accaccgtta cctgaaatca aaatcacgcc aagccctgtt cgggtaggct
     9421 ctttgaaaca acatccgagc ctgcgcttgg gtaaatcagg cgtggcagcc gcgaaacgtg
     9481 cggcgcgcaa acgcaagaat cgtc
//
LOCUS       DAXHPG010000069         9462 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXHPG010000069 DAXHPG010000000
VERSION     DAXHPG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.069"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(158..1600)
                     /gene="aldA"
                     /locus_tag="ACFM6F_001227"
     CDS             complement(158..1600)
                     /gene="aldA"
                     /locus_tag="ACFM6F_001227"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFC6164636.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVST
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1635..1835)
                     /locus_tag="ACFM6F_001228"
     CDS             complement(1635..1835)
                     /locus_tag="ACFM6F_001228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164637.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTGANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTVIYLGIN"
     gene            complement(1844..2233)
                     /locus_tag="ACFM6F_001229"
     CDS             complement(1844..2233)
                     /locus_tag="ACFM6F_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164638.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTVDRISVSFIREIQAA"
     gene            complement(2441..3358)
                     /locus_tag="ACFM6F_001230"
     CDS             complement(2441..3358)
                     /locus_tag="ACFM6F_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6164639.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRRRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWNLSPNLRGSAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFNRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3530..4330
                     /locus_tag="ACFM6F_001231"
     CDS             3530..4330
                     /locus_tag="ACFM6F_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6164640.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4379..5155
                     /gene="mlaE"
                     /locus_tag="ACFM6F_001232"
     CDS             4379..5155
                     /gene="mlaE"
                     /locus_tag="ACFM6F_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC6164641.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5206..5700
                     /gene="mlaD"
                     /locus_tag="ACFM6F_001233"
     CDS             5206..5700
                     /gene="mlaD"
                     /locus_tag="ACFM6F_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC6164642.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5737..6327
                     /locus_tag="ACFM6F_001234"
     CDS             5737..6327
                     /locus_tag="ACFM6F_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC6164643.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6384..6662
                     /locus_tag="ACFM6F_001235"
     CDS             6384..6662
                     /locus_tag="ACFM6F_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC6164644.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTAAAFTRFRQQCRLKDTRAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6659..7486
                     /locus_tag="ACFM6F_001236"
     CDS             6659..7486
                     /locus_tag="ACFM6F_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC6164645.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7611..7973
                     /locus_tag="ACFM6F_001237"
     CDS             7611..7973
                     /locus_tag="ACFM6F_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC6164646.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7973..8356
                     /locus_tag="ACFM6F_001238"
     CDS             7973..8356
                     /locus_tag="ACFM6F_001238"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6164647.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            8353..8841
                     /locus_tag="ACFM6F_001239"
     CDS             8353..8841
                     /locus_tag="ACFM6F_001239"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC6164648.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8922..9050)
                     /locus_tag="ACFM6F_001240"
     CDS             complement(8922..9050)
                     /locus_tag="ACFM6F_001240"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC6164649.1"
                     /translation="MAGVTVGRGSVVGAGAVVTKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(9056..9436)
                     /locus_tag="ACFM6F_001241"
     CDS             complement(9056..9436)
                     /locus_tag="ACFM6F_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC6164650.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGAG"
BASE COUNT         2175 a         2640 c         2382 g         2265 t
ORIGIN      
        1 gcggcttcgt cgccttgtcc tgatttttgt taatccacta tacaaagaca aaaacatcga
       61 cctcggtcat gactgattgc cggtgaagca ataaaaatgc cgtctgaacc tgaaaacgag
      121 ctcagacggc attttctatc ggggttttaa agcggtatta aatatcggtt tccagataaa
      181 cgacttgggt ttgcagatat tcttccaaac cgtgtttgcc gtccgcgccg ccgataccgg
      241 attttttcca accggcgtgg aaaccctgca tcgcttcaaa gttttcgcgg ttgatgtagg
      301 tttcgccgaa ttgcaggcgg cgggtaacgt agaaggcttc gtttaaatta gtcgtataaa
      361 cagaactggt cagaccaaac tcgcaatcgt tagccagtgc gatgacttgg tcgagcgtgt
      421 cgaaagtgga aacaggcagg acggggccga aggtttcttc tttcataatg tccatactgt
      481 tgtcggtgtc ggtcagcagg gtcggctcga agaaatagcc gcgtccttcg gcgcgtttgc
      541 cgccgcaaac caatttcgca ccttgtttga ctgcccgttc cactttttcg gcaacggctt
      601 tgacggcgcg ctcttcaatc agcgggccca tttccagcgc gccggcttcg gcttcggcag
      661 ggttgccgta gcgcacgcct ttcatcgcgg cggtcatttt ttcgataaac gcgtctttca
      721 ggctgctgtg gacatagacg cgctcggcgc agttgcagat ttgaccagtg ttgccgacgc
      781 gcgaagccaa gatggatttc accgccaaat ccaaatccgc gtctttcaaa acgatggcgg
      841 gggctttgcc gccgagttcc agcgaaactt tggtgatgtt ggcggaagcg gcttccatca
      901 cttggcggcc tgcttcgacg gagccggtca ggctgaccat atcgacttgc ggatgggcag
      961 acaaggcatt gccgatttcc gcacccggac cgttcaccac gttgaacacg cctgcgggca
     1021 atccgaccgc atcgacgatt tcggcgaaga tgtggcagtt gatcggggtt acgctgctgg
     1081 gtttgacaac gatggtgttg cccgttacta aggcgggacc cattttgcgg gcaatcagga
     1141 agaaagggaa gttccacggc aaaatgccgg caattacgcc cagcggacgt ttgaacaata
     1201 aaatattttc acgcgggcgg tcgctttgga tgatttcgcc ttcgtaacgg cgcgcccatt
     1261 cggcttggta atcgagatag tcggcggtga acatcacttc tacgcgcgcc aagtctttgg
     1321 ttttgccgcc ttcggcaacg atggtgtcgg tcagctcgtc ggcgcgtccg cgtatgcctt
     1381 gggcgatttt gcgcaaatac gcgccgcgtt cgaccgcagg caggcgttcc caagccggtt
     1441 gcgccgcgcg tgccgccgcc acagctcggt caacgtctgc ttttccgcct ttgggttcgc
     1501 gggcgatggt ttcttcggtg gaagggttca atacgtcgcg ccattcgccg ttgaagtcgt
     1561 tttcaaagcg tccgttgatg tacatggcta attgtttcat ttcgggttct ccagttttgt
     1621 agtcagatgt agttttagtt tattcccaaa taaattaccg tacaagtttc ttttgaaatg
     1681 tttagacgag aagctactat gtctgcatat tgttctgcta atttatattc actcgaatgc
     1741 aactgccgat tattccaatc tgcattcgca ccagtaaaag ccccagcatt gccgcctgtg
     1801 gcataaccaa tcgctaaact gccaacggta tccacaacag gtttcaggct gcctgaattt
     1861 ctctaataaa tgaaacactt attctatcaa ccgttaatgt aaccaaacaa ttatcaagat
     1921 aagagtagtt tattaaatac tcatcttctt caaattgtat attttgtagg gttagatgat
     1981 tattttttaa ttctgccgtt atttcatact tgaaactatc atctattttt ctaaatccaa
     2041 tatttgaagt tgatttatcc agtatgtaat cattaaagat ttcataggat aattccactt
     2101 cataaaattg attttcaaat attttgaatg ggcagtacga tataaaacaa tgtagttcat
     2161 aaccatttat ttcaatcaat actgcctctt caatggtgtt atctaacttt ttgattttac
     2221 atttatataa catttatctt gcctttggat aaattgtttt gttttttgga ttgttattca
     2281 aagttctcca aaagtctttt cctgattttg tatgtatcgt ggtaatacta ccattttcat
     2341 tggttccaac aaaaataaat ttagattgac ctttggaatc agtacccaaa aactgaacat
     2401 atccagacat gcggatttcg ggttttaaat caaatgcggc ttatcggttt tcttttttgg
     2461 tttccgcagg cgtgctgccg aaatattgcc tgaatgcctg agtaaagctg gaaacgtggc
     2521 ggtagccgca caaatacgcg gtttcgccta tgcttttgcc cccgttttgc aacaggtaga
     2581 gcgcgtgctg catttgtttg tggtgcagcc actcgcttgc ggtaataccg aaatggtcgc
     2641 gcatacggcg ttgcagcgtc ctttcactga tgttcagcgc gtctgtcagc cggttgactt
     2701 ggtgtgcgcc gtcggcaaac gcggcattta gggtgcggtt gaagtcttca gacggcatag
     2761 cgtctgcttc cgccgtttgc cccgccgccg gcccgatgcc gtctgaaacc gtgtcccaca
     2821 agtccgacag cagccgcaaa acgtccgcct cgcggcgcaa tgtttcgccc aaatgcccct
     2881 ttgggacagc tttcaggcag gatgccgccg agccgcgcag gtttggggac aaattccata
     2941 ttctgaccgg ttcgcggtaa agcaggggtg cgaaacgcgc atactccgga cgcagcagcc
     3001 attgttccat acctttaatg gtcattttga ccgttttgcc gcctcggtaa agatagcggc
     3061 tgaacaggac ctcttccccg acagcaatta ggacgatctt gccgccatcc gcatcgattt
     3121 ggaagcggcg gcggttgatg ccgaagtcca aactgccttc cagcaagagc acgaacgaca
     3181 cataaggttc ggcgaggcgg ctgctgcaaa aatcgcaccg tgccgttacc gtgccgccgt
     3241 ggatggaaat gccgttggac aaagtatcga aacggtagct gccctccaca tagctgccgc
     3301 cgccgctttt gaccagattg acgaaccggc tgctgttcaa atgttctgct ttttccatag
     3361 ttttctgata aaggttatca tttgaaagat aacatttttc gccacagcaa acaatccgcc
     3421 gccacaacgg cattttcaca acatttaccg tccgtatgcc gtctgaaaat tgcccgtctt
     3481 cccgataagc cttaaaatac acgccgtcaa acctaccgtc cgtccgtaca tgagcccatc
     3541 cccctttatc gaaatgaaag acgtcgcctt cgcgtatggc gaccgcccga ttctgaagaa
     3601 catcaatttc agcattccgc aaggcaattt tgctgccgta atgggcggtt cgggcagcgg
     3661 caaaaccacg ctgatgcgac tgattacagg acagattcgt ccgcagtccg ggcaggtttt
     3721 gattgaagga cgggatttgg cgggcttttc ggctgacgag ttgtatgaac accgccgccg
     3781 tatgggcgta ttgttccaac acggcgcgct gtttaccgat ttgtctgtat ttgacaatat
     3841 tgcttttccg atgcgcgaac tgacgcgcct gccggaagcg gtgattcgag atttggtttt
     3901 gttgaaactg aacgcggtcg ggctgcgcgg cgtggaaaac ctgatgccgt ccgagttgtc
     3961 cggcgggatg tcgcgccgcg tcgcgcttgc ccgcacaatc gcgctcgatc ccgaaattat
     4021 gttgtacgac gagccgttta ccggtctcga tccgatttcc ttgggcgtga ttgcccactt
     4081 gatcagccgc gtcaacaagg ctttgcgttc gaccagtatt atggtcacgc acgacattga
     4141 aaaatctttg gaaatcgtcg atcaggtgat tttcttggcg cacggcgaaa ttatgttctc
     4201 cggctcgccg caggaaatgc gcgaactgga ttcgccttgg gtgcgccagt ttgtcggcgg
     4261 gctggcagac ggccccgtgg cataccgtta tccggcgcaa acgtcgttgc agcaggattt
     4321 gctcgggtaa attgagccgg tgccgtctga aaccgttgaa ctttacatat agaaatttat
     4381 gaattttatc cgttccgtcg gggcgaaaac cctcggcctt attcaatccc tcggcagtat
     4441 tacgctgttt ctgctgaaca ttctggcgaa gtccggtacg gctttcgtcc gtccgcgcct
     4501 gagcgtgcgc caagtgtatt ttgccggcgt gctgtcggtg ctgattgttg ccgtttcggg
     4561 gctgttcgtc ggcatggtct tgggtttgca gggctatacg cagttgtcga aattcaaatc
     4621 cgccgatatt ttgggctata tggttgcggc ttcgctgttg cgcgaactgg gtccggtgtt
     4681 ggcggcgatt ctgtttgcca gcagcgcggg cggtgcgatg accagcgaaa tcggtttgat
     4741 gaaaacgacc gaacagctcg aagcgatgaa cgtgatggcg gtaaaccccg tcgcccgagt
     4801 ggttgcgccg cgcttttggg cgggcgtgtt ttccatgccg cttttggctt cgattttcaa
     4861 cgtggcgggt attttcggcg cgtatttggt cggtgtaacc tggctgggct tggacagcgg
     4921 tattttctgg tcgcaaatgc agaacaatat cacgatacat tacgatgtaa tcaacggtct
     4981 gattaaatcc gccgcgttcg gcgtggcggt aacgctgatt gccgtgcatc agggcttcca
     5041 ttgcgtcccg acttcggaag gcattttgcg cgccagcacg cgcacggtgg tttcgtccgc
     5101 cctgacgatt ttggcggtcg attttatatt gaccgcgtgg atgtttacag attgacaggc
     5161 cgtctgaaga cgaaacgcgg aacatcggat attcaaggaa ctttaatgaa aaagaacata
     5221 ttggaatttt gggtcggact gttcgtcctg attggcgcgg cggcggttgc ctttctcgct
     5281 ttccgcgtgg ccggcggtgc ggcgttcggc ggttcggaca aaacctacgc cgtttatgcc
     5341 gatttcggcg acatcggcgg tttgaaggtc aatgcccccg tcaaatccgc aggcgtattg
     5401 gtcgggcgcg tcggcgctat cggacttgac ccgaaatcct atcaggcgag ggtgcgcctc
     5461 gatttggacg gcaagtatca gttcagcagc gacgtttccg cgcaaatcct gacttcggga
     5521 cttttgggcg agcagtacat cgggctgcag cagggcggcg acacggaaaa ccttgctgcc
     5581 ggcgacacca tctccgtaac cagttctgca atggttctgg aaaaccttat cggcaaattc
     5641 atgacgagtt ttgccgagaa aaatgccgac ggcggcaatg cggaaaaagc cgccgaataa
     5701 acgtatgcca tcatattcaa actaggacac ttaatcatga aaaaatcctc cctcatcagc
     5761 gcattgggca tcggtatttt gagcatcggc atggcatttg ccgctcctgc cgacgcggta
     5821 aaccaaatcc gtcaaaacgc cactcaagta ttgagcatct taaaaagcgg tgatgccaac
     5881 accgcccgcc aaaaagccga agcctatgcg attccctatt tcgatttcca acgtatgacc
     5941 gcattggcgg tcggcaaccc ttggcgcacc gcgtccgacg cgcaaaaaca agcgttggcc
     6001 aaagaatttc aaaccctgct gatccgcacc tattccggca cgatgctgaa attaaaaaac
     6061 gccaacgtca acgtcaaaga caatcccatc gtcaataaag gcggcaaaga aatcatcgtc
     6121 cgcgccgaag tcggcgtacc cgggcaaaaa cccgtcaaca tggacttcac cacctaccaa
     6181 agcggtggta aataccgtac ctacaacgtc gccatcgaag gcgcgagcct ggttaccgtg
     6241 taccgcaacc aattcggcga aatcatcaaa gcgaaaggcg tggacggact gattgccgag
     6301 ttgaaggcta aaaacggcag caagtaatcc ataagccatg ccgccagact gaagatggca
     6361 tggatttttt cctaccgaac actatgcata cagaactcaa caacggaaca ctgcacatca
     6421 gcggcgacgt aaccgtcaaa accctgaccg ccgccgcctt tacccgcttc agacagcaat
     6481 gtcgtctgaa agacacccgc gcggttgatt tgagcggcgt cggacgcgcc gattccgcct
     6541 gcgtgtcgct gctgctcgaa gtgctgcgcg gttgcaaagg cagcgtcagg ctgaccggca
     6601 ttcccgaatc cgtgcgcgcg ctgtccgaac tgtacgaaat caaagactgg ctgaaatcat
     6661 gaaaaaaacc gcctatgcct tcctcctgct gatcgggttc gcttccgccc ctgcatttgc
     6721 cgaaacccgc cccgccgacc cttatgaagg ctacaaccgc gccgttttca aattcaacga
     6781 ccaagccgac cgctacattt tcgcccctgc cgcgcgcggc taccgcaaag ttgcgccgaa
     6841 acccgtccgc gccggcgtgt ccaatttttt taacaacctg tgcgacgtgg tcagcttcgg
     6901 cagcaatatc ttgcgcttgg acatcaaacg cgcaagcgaa gaccttgtcc gcgtcggcat
     6961 caacaccact ttcggtttgg gcgggcttat cgacatcgcc ggcgcgggcg gcattcccga
     7021 caataaaaac accttgggcg acacgtttgc ttcgtgggga tggaaaaaca gcaattattt
     7081 cgtgttgccc gtcttagggc cgtccaccgt ccgcgacgcg ctcggcacgg gtattacctc
     7141 cgtttattcg cccaagaata tcgtcttccg cacccctgtc ggacgctggg gcacgactgc
     7201 cgtatccgcc gtcagtacgc gcgaaggtct gctcgatttg accgacagtc tggacgaagc
     7261 cgccatcgac aaatacagct acacgcgcga cctctatatg aaagtccgtg cgcggcagac
     7321 cggtgcaaca cctgccgaag gtacggaaga taacatcgac atcgacgaat tggtcgaaag
     7381 tgccgaaacc ggcgcggcgg aaactgccgt tcaagaagat tccgtatccg aaacacaggc
     7441 agaagcagca ggggaagccg aaacgcaacc tggaacacaa ccttaaagcc atgccgtctg
     7501 aaaatccgtg ttcggacggc attcccttct gtcggaacgg acagtccgaa accacagatt
     7561 gggctataat ctcttttttg cgcataccgc gcgacagcaa aggaaaaacc atgtacgaag
     7621 tcaaccgcag cgtattcgtc ctcatccctt tagaaccttt ttggaactgg ctgcaaaccc
     7681 tgccgggcaa ccacctcgac ggactgactc ttgaagacat ccaagccgac gccaattcct
     7741 accttgtccg cccgtgcgaa accgccgacg aagtatggga cgaaatcgaa gcccgttttg
     7801 aagacatttt cgccgccgaa cttgccgact ggtgcgaaga cgaacgcgag tggcctgcgc
     7861 tcgatgccga cattttcaac gaatggttcg acatccagct ttctaccgtc atcaccgacc
     7921 tcgaacacga accgcttgcc cgcgaagcct tccaacccat caacctgaac tgatgaagct
     7981 gaccgtccgc aactaccatc tcgacggcta cggccatgtc aacaatgcgc gctacctcga
     8041 atttttcgaa gaagcgcgct gggcgttttt tgaagaacgc ggactgctgc acgagttggc
     8101 aggcctcata ctgattgtcg cccgcatcga catccgatac agccgccccg ccgtcgaagg
     8161 cgacgtattg cagttttcat gccgcctgaa aacccccggc acgcgccgta ttgtgctgac
     8221 ccagacgatt acactgccaa acggcaaaac cgccgcagaa gccgacatca ccctgatgcc
     8281 cgtccacgcc gccacacaac gcaccgtcag cctgcccgcc acccttgccc gcgcattgga
     8341 agccttgtcc gaatgaaaaa aatactcacc gccgccgtcg tcgcactgat cggcatcctc
     8401 cttgccatcg tcctcatccc cgacagcaaa accgcgcccg ccttctccct gcccgacctg
     8461 cacggaaaaa ccgtttccaa cgccgacctg caaggcaaag taaccctgat taatttttgg
     8521 tttccctcct gtccgggttg tgtgagcgaa atgcccaaaa tcattaaaac ggcaaatgac
     8581 tataaaaaca aaaacttcca agtccttgcc gtcgcccagc ccatcgatcc gatagaaagc
     8641 gtccgccaat atgtcaaaga ctacggtttg ccgtttaccg tcatgtatga tgcggacaaa
     8701 gctgtcggac aggcgttcgg cacacaggtt tatccgactt ccgtccttat cggcaaaaaa
     8761 ggcgaaatcc tcaaaactta tgtcggcgaa cccgatttcg gcaaactcta ccaagaaatc
     8821 gataccgcgc tggcgcaata gcgatgccgt ctgaagcgca aaaaacagaa acgatgttcc
     8881 tgttttttca gatcatccgc cccgacacgt tcagacggca ttcaaccttc cggcagattc
     8941 tttttcacca ctgccggatt gcctgccgcc aaagagtagg gcggaatgtc ttttgtaacc
     9001 accgcgcctg cgcccacgac cgaaccgcgt ccgacggtta cgcccgccat tacaatcacc
     9061 ctgcgccccg gccagacatc gtcctccaac gtaatcggac ggatttccgt gtagccctca
     9121 aagcgttttt tgaacggtca aacttgtggt tatttgaata aaacagacat tccggctcca
     9181 tcatcacatt tttgccgacc accggcccac ggcagatttc acagtttgcc ccgatgcccg
     9241 agccgtcgcc caaaaccgta tccggaaaca catacgcccc gcgttcgata ttgaccccgc
     9301 gtccgatatg cggagaaacc cgccgcgcca aaaaaccgcg tacccgtctg ccgacgatgc
     9361 cgatgccgcg catatgtgac ggcggcaaca ccgaaccggc aaaacgcagc agcaggaaaa
     9421 agaattttga tcccatcaaa cctccctcaa aagaactttt ta
//
LOCUS       DAXHPG010000070         9321 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXHPG010000070 DAXHPG010000000
VERSION     DAXHPG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.070"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            164..1507
                     /locus_tag="ACFM6F_001242"
     CDS             164..1507
                     /locus_tag="ACFM6F_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC6164651.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1510..2742
                     /locus_tag="ACFM6F_001243"
     CDS             1510..2742
                     /locus_tag="ACFM6F_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC6164652.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2735..3511
                     /locus_tag="ACFM6F_001244"
     CDS             2735..3511
                     /locus_tag="ACFM6F_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC6164653.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSIIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKNTDISKTFAERIEQRVVDLATGEYVADAPKDFSARVAGKDPA
                     QSIQIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3511..4137
                     /locus_tag="ACFM6F_001245"
     CDS             3511..4137
                     /locus_tag="ACFM6F_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC6164654.1"
                     /translation="MADMKRLKHLMFSPFINNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4141..4734
                     /gene="nqrE"
                     /locus_tag="ACFM6F_001246"
     CDS             4141..4734
                     /gene="nqrE"
                     /locus_tag="ACFM6F_001246"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC6164655.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4748..5965
                     /gene="nqrF"
                     /locus_tag="ACFM6F_001247"
     CDS             4748..5965
                     /gene="nqrF"
                     /locus_tag="ACFM6F_001247"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC6164656.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPSHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6117..7172
                     /locus_tag="ACFM6F_001248"
     CDS             6117..7172
                     /locus_tag="ACFM6F_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6164657.1"
                     /translation="MPSETRLPNFIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRGKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAYVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            7198..7413
                     /gene="nqrM"
                     /locus_tag="ACFM6F_001249"
     CDS             7198..7413
                     /gene="nqrM"
                     /locus_tag="ACFM6F_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC6164658.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7537..8115)
                     /gene="grpE"
                     /locus_tag="ACFM6F_001250"
     CDS             complement(7537..8115)
                     /gene="grpE"
                     /locus_tag="ACFM6F_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC6164659.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8299..9117
                     /gene="cysE"
                     /locus_tag="ACFM6F_001251"
     CDS             8299..9117
                     /gene="cysE"
                     /locus_tag="ACFM6F_001251"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC6164660.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2263 a         2586 c         2301 g         2171 t
ORIGIN      
        1 aacagcttcc gcaaggaaaa aagcagcaaa tctgacaaac aggtacaatt cctgatagaa
       61 tctaacactt tgctacatta atttattcaa tgcttttgat gccacgcgac acggtatcaa
      121 aacacgccgt ttttacctta accatcacaa gtaatgcaaa tatatgatta aaatcaaaaa
      181 aggtctaaac ctgcccatcg cgggcagacc ggagcaagtc atttatgacg ggcccgtcat
      241 taccgaagtc gcgttgcttg gcgaagaata tgccggtatg cgcccctcga tgaaagtcaa
      301 ggaaggcgat gccgtcaaaa aaggccaagt gctgtttgaa gacaaaaaga atccgggcgt
      361 ggtgtttacc gcgccggttt caggcaaaat cgccgccatc catcgcggcg aaaagcgcgt
      421 gcttcagtcg gttgtgattg ccgttgaagg caacgacgaa atcgagttcg aacgctacgc
      481 gcccgaagcg ttggcaaact taagcggcga agaagtgcgc cgcaatctga ttcaatccgg
      541 tttgtggact gcgctgcgta cccgtccgtt cagcaaaatc cctgccgtcg atgccgagcc
      601 gttcgccatc ttcgtcaatg cgatggacac caatccgctg gcggcagacc ctgtggttgt
      661 gatcaaagaa gccgccgagg atttcagacg aggtttgctg gtattgagcc gtttgaccga
      721 gcgtaaaatc catgtgtgta aggcagctgg cgcagacgtg ccgtctgaaa atgctgccaa
      781 catcgaaaca catgaattcg gcggcccgca tccggccggt ttgagtggca cgcacattca
      841 tttcattgag ccggtcggtg caaacaaaac cgtttggacc atcaattatc aagatgtaat
      901 tgccatcgga cgtttgtttg caacaggccg tctgaacacc gagcgcgtga ttgctttggg
      961 tggttctcaa gtcaacaaac cacgcctctt gcgtaccgtt ttgggtgcga aagtatcgca
     1021 aattactgcg ggcgaattgg ttgacgcaga caaccgcgtg atttccggtt cggtattgaa
     1081 cggcgcgatt acacaaggcg cgcacgatta tttgggacgc taccacaatc agatttccgt
     1141 tatcgaagaa ggccgcagca aagagctgtt cggctgggtt gcgccgcagc cggacaaata
     1201 ctccatcacg cgtacgaccc tcggccattt cctgaaaaac aaactcttca agttcacgac
     1261 agccgtcaac ggtggcgacc gcgccatggt gccgattggt acttacgagc gcgtaatgcc
     1321 gctggacatc ctgcctaccc tgcttttgcg cgatttaatc gtcggcgata ccgacagcgc
     1381 gcaggcattg ggttgcttgg aattggacga agaagacctc gctttgtgca gcttcgtctg
     1441 cccgggcaaa tacgaatacg gcccgctgtt gcgtaaggtg ctggaaacca ttgagaagga
     1501 aggctgatta tgggcttgaa acattttctg gaaaaaatcg aaccgcactt cctgccgggc
     1561 ggcaaacatg aaaaatggta tgccctctac gaagctgcgg cgacgatttt ctatacatcc
     1621 ggtgcggtaa cgcgcaaagc ggcacacgtc cgcgatgcgc tcgactccaa acgcatgatg
     1681 attttggtgt ggctggcttt gttccctgcc atgttctacg gtatgtacaa cgtcggcgca
     1741 caggcattcg gtgcgttaac gcccgatttg ctgcaacaaa gcatcgccaa cgactggcat
     1801 tacgcccttg ccaacgcttt gggcatcaat atgtcgtctg aagcgggcgt gttgggcaaa
     1861 atgctgttcg gcgcgattta cttcctgccg atttacgcga ccgtatttat tgtcggcggt
     1921 ttctgggaag ttttgttcgc caccgtgcgc aaacatgaaa tcaacgaagg tttctttgtt
     1981 acctcgattc tgtttgcctt aatcgttccg cccacgctgc cgctgtggca ggcagctttg
     2041 ggtatttctt tcggcgtggt ggttgcgaaa gaagtattcg gcggtacagg taaaaacttc
     2101 atgaaccctg cgctggcagg ccgtgccttc ctgttcttcg cctaccctgc caacttgagc
     2161 ggcgatgcgg tttggacggc ggttgacggc tattccggcg caaccgcgct ggcgcaatgg
     2221 gcggcacacg gtgcagacgg cctgaaaaac gccgtaaccg gtcaaaccat cacttggatg
     2281 gatgcgttta tcggcaaact gcccggctcc atcggcgaag tatccacttt ggcactctta
     2341 atcggcggcg cgtttatcgt gtttgcccgc atcgcttctt ggcgcattat tgccggcgtg
     2401 atgatcggta tgattgccat gtcttcgctg ttcaacttca tcggttcgga caccaacgct
     2461 atgtttgcta tgccttggta ctggcatttg gtcgtcggcg gcttcgccat cggtatgctg
     2521 tttatggcaa ccgaccccgt ttctgcttcc tttaccaatg tcggcaaatg gtggtacggc
     2581 gcgctaatcg gtgtgatgtg cgtattgatc cgtgtggtca atccggctta ccccgaaggc
     2641 atgatgttgg cgattctgtt tgccaacctg tttgccccga ttttcgacta tttcgtcgca
     2701 caagcgaaca tcaaacgcag aaaggcgcgc agcaatggct aagaaattcg ataaagacag
     2761 cttcagcggc acgctgattg tcgtgttggc ggtcagcctg atttgttcca tcatcgttgc
     2821 tggtgcggtc gtcggtttga aacccatcca agagaaacaa aaactccaag acaaacaagg
     2881 ctatatcttg agcgtagccg gtttgatgga taaaaacacc gacattagca aaacctttgc
     2941 cgagcgtatc gagcaacgtg ttgtcgattt ggcgaccggc gaatatgtcg ctgatgcgcc
     3001 taaagacttc agcgcacgcg ttgccggcaa agaccctgcc caaagcatcc aaatcaaacc
     3061 tgaagacgat ttggcaggca tcaaaagccg tgccaaatac accgaggttt atttggtaaa
     3121 aggcgaagac ggcaaaatcg gccaaatcat cctgccgatg cacggcaacg gtttgtggtc
     3181 ggtcatgtac ggctttgtcg ccatccaacc cgacggcaac accatcaacg gcattaccta
     3241 ctacgagcaa ggcgaaaccc cgggcttggg cggcgaaatc ggcaatcctt tgtggcagca
     3301 aaaattcgtc ggcaaaaaac tgtttgacgg acaaggcaaa ctcgccctgc acgtcggcaa
     3361 aggcgcgggt tcggacaaag aacacggcgt agatgccctc tccggtgcat cgctgacatc
     3421 caaaggcgtg caaggttcgt tcgcctactg gttcggcgaa aacggctata tcccctacct
     3481 gaacaaattg aaatcagcag gagcgcaata atggctgata tgaaacgctt gaaacatttg
     3541 atgttttcac cctttatcaa caacaacccg attgccttgc aggttttggg tatttgttcg
     3601 gctttggcgg ttaccaccaa acttcagacg gccatcgtga tgggtatttc cgtagctttg
     3661 gtaaccggtt tttccagctt cttcatttcg ctggtacgca actacatccc caacagcatc
     3721 cgtattatcg tgcaaatggc gattatcgcg tcgctggtta cgctggtcga ccaactcttg
     3781 caggcatttg cctacgaatt gtccaaacag ctttccgtat tcgtcggtct gattattacc
     3841 aactgtatcg tgatgggccg cgccgaagca tttgcgatga aagagccgcc gctggaaagc
     3901 ctgatcgacg gcatcggcaa cggcgcgggc tacgggataa tgctgcttgt cgtcgccacc
     3961 gtccgcgaac tgattggctc gggcaaactc ttgggctaca ccgttttcca aaccgtgcag
     4021 gacggcggct ggtatcagac caacggcttg ttcctgctcg cccccagcgc gttcttcatc
     4081 atcggctttt tgatttgggg actgcgtacc tggaaacccg aacaggcgga ggaataagct
     4141 atggaacact atttgagcct cttcatcaaa tccgtcttca ttgaaaatat ggcactatcc
     4201 ttctttttgg gtatgtgcac gtttttggcg gtatccaaaa aagtatccac cgcattcggt
     4261 ttgggtgtgg cggtaatttt cgtactcggg ctgtccgtcc ctgtcaacca actcgtttac
     4321 tcgctgctca aagacggcgc gattgtcgaa ggcgtggatt tgaccttttt gaaattcatc
     4381 accttcatcg gcgtgattgc cgctttggtg cagattttgg aaatgttctt ggataaattc
     4441 gtccccgccc tctataacgc attgggcatc tacctgccgc tgattaccgt gaactgcgcg
     4501 attttcggcg ccgtatcgtt tatggcgcaa cgcgaataca acttcggcga atccgttgta
     4561 tacggcttcg gcgcgggctt gggctggatg ttggcgattg tcgctttggc gggcattacc
     4621 gaaaaaatga aatattcgga cgctcccaaa ggcctcaaag gcttgggcat tacctttatc
     4681 gccgccggcc tgatggcgat ggcgtttatg tcgttctccg gcatccagtt ataagaaagg
     4741 gatcggtatg gagattattt taggtatcgt gatgtttacc gtcatcgttt tggttttggc
     4801 actgatgatt ctgtttgcca aatccaaact ggtcagcgaa ggcgacatca ccatcaaagt
     4861 caacggcgaa aaagagctga cgatgcccgc cggcggcaaa ctcttgggcg cgcttgccaa
     4921 cgaaggcatc tttatcccct ccgcctgcgg cgggggcggt tcgtgcggac aatgccgcgt
     4981 cgtcgtaaaa agcggcggcg gcgacatcct gccgaccgag ctgtcccaca tcagcaaacg
     5041 cgaagcacgc gaaggctgcc gtctgtcttg tcaggtcaac gtcaaaaccg acatggacat
     5101 cgaagtcccc gaagaagtgt tcggcgtgaa aaaatgggaa tgcaccgtca tctccaacga
     5161 caacaaagcc acgttcatta aagaactcaa gcttgccatt cctgaaggcg aagaagttcc
     5221 tttccgcgcc ggcggctaca ttcaaattga agccccttct cacactgttg cctacaaaga
     5281 cttcgacatt cctaaggaat accacgaaga ctgggacaaa tacaatctgt ggcaatacgt
     5341 ttccaaagtg gatgagccga ttttgcgcgc ctactctatg gcttcgtatc ctgaagaaaa
     5401 aggcatcatt atgctgaacg tgcgtatcgc cacgccgccc ccgcgcgtac ccgatgcgcc
     5461 tccgggacaa atgtcttcct acatctggtc gctcaaaccc ggcgacaaag ttacgatttc
     5521 cggcccgttc ggcgaatttt tcgccaaaga caccgatgcc gaaatggtat ttatcggcgg
     5581 tggtgcgggt atggctccga tgcgttccca cattttcgac cagttgaaac gtttgaactc
     5641 caaacgcaaa attaccttct ggtatggcgc acgttccaaa cgcgagatgt tctatgtcga
     5701 agactttgac caactcgcag cagaattccc gaacttcacg tggcacgtcg ccctgtccga
     5761 tccactgccg gaagacaact gggacggcta cacaggcttc attcacaacg tggtttacga
     5821 aaaccatctg aaaaaccacg aagcgccgga agactgcgaa ttttatatgt gcggcccgcc
     5881 gattatgaac cagtccgtca tcaaaatgct caaagacttg ggtgtggaag acgaaaacat
     5941 cctcttggac gatttcggcg gttaaatatt cggcttcaaa cacaaaagga agaaacctcg
     6001 gtttcttcct ttttttatcc gataccgtcc gaacaccttt cagacggcat caacataaaa
     6061 acataccggc cgttttgcga tataattcaa gccatttgcc aagactatca aacactatgc
     6121 cgtctgaaac acgcctgccg aactttatcc gcgccttgat atttgccctg ggttttatct
     6181 tcctaaacgc ctgttcggaa caaaccgcgc aaaccgttac cctgcaaggc gaaacgatgg
     6241 gcacgaccta taccgtcaaa tacctttcaa ataatcgggg caaactcccc tcccccgccg
     6301 aaatacaaaa gcgcatcgat gacgcgctta aagaagtcaa ccggcagatg tccacctatc
     6361 agcccgactc tgaaatcagc cggttcaacc aacacacagc cggcaaaccc ctccgcattt
     6421 caagtgactt cgcatacgtt accgccgaag ccgtccgcct gaaccgcctg acgcacggcg
     6481 cactggacgt aaccgtcggc cccttggtca acctttgggg attcggcccc gacaaatccg
     6541 ttacccgtga accgtcgccg gaacaaatca aacaggcggc atcttatacg ggcatagaca
     6601 aaatcatttt gaaacaaggc aaagattacg cttccttgag caaaacccac cccaaggcct
     6661 atttggattt atcttcgatt gccaaaggct tcggcgttga taaagttgcg ggcgaactgg
     6721 aaaaatacgg cattcaaaat tatctggtcg aaatcggcgg cgagttgcac ggcaaaggca
     6781 aaaacgcgcg cggcgaaccg tggcgcatcg gtatcgagca gcccaatatc gtccaaggcg
     6841 gcaatacgca gattatcgtc ccgctgaaca accgttcgct tgccacttcc ggcgattacc
     6901 gtattttcca cgtcgataaa aacggcaaac gcctctccca tatcatcaac ccgaacaaca
     6961 aacgacccat cagccacaac ctcgcctcca tcagcgtggt cgcagacagt gcgatgacgg
     7021 cggacggctt gtccacagga ttattcgtat tgggcgaaac cgaagcctta aagctggcag
     7081 agcgcgaaaa actcgctgtt ttcctgattg tcagggataa aggcggctac cgcaccgcca
     7141 tgtcttccga atttgaaaaa ctgctccgct aaacatacaa accgattagg aaacaccatg
     7201 aaaaccctgc tccttacctt cggcatcttc ctgaccgtca tcatcggtat ggcggtcggc
     7261 tatattttct ccaaacgcag catcaaaggc agttgcggcg gcattactgc cttgggtatg
     7321 aaaaaaatgt gcgactgcga cacaccttgc gacaccctgc aaaagaaact ggatgaagaa
     7381 aaacaggcag gcggcatacg ggttgaccgt taacgccgaa acaaaatgcc gtctgaagcc
     7441 cgacaggcgg gttcagacgg cattgccgct ttacttcgat cggtttatac gcaaaacgcg
     7501 cttcaggaaa taaatcagat tattccccag acgccttcag gcttccttct gtgccaccgt
     7561 aaccatagcc gggcgcaaca cgcggtcgga cagcgtataa cccttcttca tcacacccac
     7621 cacggtattc ggctcctgtt cgcttgccac cgcctgcatc gcctgatgga tattcggatc
     7681 gagcttatcg cccgctttag ggttgatttc cttgatttgc gtagcatcaa atgctttctg
     7741 caactcgttc aaagtcatct gcacgcccat tttcagcgca tcgaaattgc cgctctgatc
     7801 caaaagcgcc atttccagat aatccttgac cggcagcatt tccacggcaa acttctgtcc
     7861 ggcgaacttg tgcgtatccg caatttcctg ctggtggcgg cggcgcaggt tttgctcgtt
     7921 tgccaaagcg cgcagctgct cgtctttcaa ctgcgcttcc agctcggcaa tccgcgcctg
     7981 caaatcctca taagccggct ctgcggcagc ctgttcctgc acaccgtccg catttcctac
     8041 tgtctcgacg gtttccaccg cctccacatt ttcaaccgct tcttcactgt tttgctgctg
     8101 tgtctgttcg ctcatatcgt atcccttaaa acaaattgga aatcaaaatc cagttattac
     8161 ccgcgcaaga taaggacatt ttcaagaaac ttcaagcaaa agcagagaat ttcaaattca
     8221 ttttcaaatc ccctaccgaa aaaataatat agacggtaag atactgccga ccatatcgat
     8281 tttcaaacgt catcactcat gaaaaaagac catttaaaca caaccggttt cgacctctgg
     8341 cacaccatcc gcgaagaaac cgcggccgcg gtctccgccg aaccgatgct ggcaagtttt
     8401 ttgcaccaaa ccgtgttgcg ccacgagtcc ctcggctccg tccttgccta ccacctttcc
     8461 agcaaactcg gcagcccgat tatggacgtg cgcgcgctgt ttgaaatcta ccagcaggcg
     8521 ttgggcagcg acacccaaat cggcaaatgc gtcgaagcag acttaaaagc catctacgaa
     8581 cgcgatcccg cctgcgacga atattcgctg ccgcttttat atttcaaagg cttccacgcg
     8641 attcaggcac accgcatcaa ccatcggctg tatctcgacg ggcgcaaaac gctggcgtat
     8701 ttcctgcaaa accgtatgtc cgaagtattc ggcgtggaca tccatcccgc cgcccgtttc
     8761 ggatacgggc tgatgctcga ccacgccacc ggctttgttg ccggcgaaac cgccgtgttg
     8821 ggcaacaata tttcgatttt gcacggcgta acgctcggcg gttcgggcaa agaaggcggc
     8881 gaccgccacc ctaaaatcgg cgacggcgtg atgatcggcg caaacgcctc gatattgggc
     8941 aatatccgca tcggcagcaa tgccaaaatc ggggcgggca gcgtcgtggt ttcagacgtg
     9001 ccgccgtcca tcacggttgt cggcgtaccc gccaaacccg tggcgcgatc gctcaaaaca
     9061 ccgtcggcgg atatggatca aaatatccag tttaccgaaa tcgactttat gatttgaata
     9121 tccgcaccaa tgccgtctga aacgccaaac gggcttcaga cgacattgcc cgcttcaagc
     9181 ctgcgccttg atatgccgcc gatgtcggca cttgccagtc ggacaaaatg ccttttattc
     9241 aatacacccg attcccacgc aaccggaaat atagtggatt aacaaaaatc aggacaaggc
     9301 gacgaagccg cagacagtac g
//
LOCUS       DAXHPG010000071         9221 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXHPG010000071 DAXHPG010000000
VERSION     DAXHPG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.071"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            105..314
                     /locus_tag="ACFM6F_001252"
     CDS             105..314
                     /locus_tag="ACFM6F_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC6164661.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            403..1617
                     /locus_tag="ACFM6F_001253"
     CDS             403..1617
                     /locus_tag="ACFM6F_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFC6164662.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1704..4283)
                     /gene="clpB"
                     /locus_tag="ACFM6F_001254"
     CDS             complement(1704..4283)
                     /gene="clpB"
                     /locus_tag="ACFM6F_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC6164663.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDIMPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4544..5554
                     /gene="trpS"
                     /locus_tag="ACFM6F_001255"
     CDS             4544..5554
                     /gene="trpS"
                     /locus_tag="ACFM6F_001255"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC6164664.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5560..5901
                     /locus_tag="ACFM6F_001256"
     CDS             5560..5901
                     /locus_tag="ACFM6F_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164665.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVVGKCMETDGKGAPSGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6223..6546
                     /locus_tag="ACFM6F_001257"
     CDS             6223..6546
                     /locus_tag="ACFM6F_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164666.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(6755..8263)
                     /gene="ppx"
                     /locus_tag="ACFM6F_001258"
     CDS             complement(6755..8263)
                     /gene="ppx"
                     /locus_tag="ACFM6F_001258"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC6164667.1"
                     /translation="MTTHPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(8379..9221)
                     /locus_tag="ACFM6F_001259"
                     /pseudo
     CDS             complement(8379..9221)
                     /locus_tag="ACFM6F_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714481.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
BASE COUNT         2116 a         2400 c         2438 g         2267 t
ORIGIN      
        1 gttaatccac tatatcgatt tgaatttttc agaaaatgaa gcggacgttt gggcggaggc
       61 aacttgtttg ataagatagc aatattttaa aacggagaaa gatcatgcct tatgtcaata
      121 ttaaagtaac cggcggcaag gaagcaccga ctgccgcgca aaaagcggaa ctgatcggcg
      181 gcgtaaccga attgctggca cgcgtgctgg gcaaaaatcc cgaaacaacg gttgtcgtga
      241 ttgacgaagt ggataccgat aactggggaa taggcggcaa aagcgtcagc gaacggcgca
      301 aagagggcag gtaaaaagcc tgaaaatctc ggtttgatgc tttaaattcc gcgtgaaaaa
      361 gagtacattc ccacccattg cccaaaattt acggaacaca tcatggataa atttcccaag
      421 tctgcaaagc tcgatcacgt ctgttacgac atacgcgggc cggttcacaa aaaagccctt
      481 cagttggaag aggagggcaa taaaatcctt aaactcaata tcggcaaccc tgcgccgttc
      541 ggctttgaag cccctgatga aatcttggtc gatgtcatcc gcaacctgcc gacttcgcaa
      601 ggctattgcg attctaaagg gctgtattcc gcccgcaaag ccattgttca ctactatcag
      661 accaaaggtt tgcgcgatat tacggtaaac gatgtctata tcggcaacgg cgtgtccgag
      721 ctgattacga tgtctatgca ggcattgctc aacgacggcg acgaaatcct gattcccgcg
      781 cccgactacc ctttgtggac ggcggcggca acgcttgcgg gcggtacggt acgccattat
      841 ctgtgcgacg aagaaaacgg ctggttcccc aaccttgccg atatggaagc caaaatcacg
      901 cccaaaacca aagccatcgt cgtcatcaat cccaataatc cgacaggcgc ggtgtacagc
      961 agggaaatcc tgttggaaat cgccgaactg gcgcgcaagc acggtttgat tattttcgcc
     1021 gacgagattt acgataaaat cctttatgac ggcgcggttc accaccacat cgccgcgctt
     1081 gcccccgacc ttttgacggt aacgttcaac ggtttgtcca aagcctaccg cgtcgccggc
     1141 ttccgccaag gctggatggt gctcaacggg ccgaaacatc atgcaaaagg ttacatcgag
     1201 ggtttggata tgctctcgtc tatgcgcctg tgtgccaata cgccgatgca gcacgcgatt
     1261 cagacggcat tgggcggcta tcagagcatc aacgaattca ttttgcccgg cggacggctt
     1321 ttggaacagc gtaacagggc gtgggaattg gtcaatcaga tacccggcgt atcctgcgtc
     1381 aaaccgatgg gcgcgatgta tatgttccca aaaatcgata ccgaaatgta ccgtatccac
     1441 gatgacatga aattcgttta cgatttgctg gtgcgcgaaa aagtcttgct ggtacaggga
     1501 acggggttta attggattaa acctgaccat ttccgcattg tcacgctgcc ttacgtccat
     1561 cagattgaag aggcgatggg caggttggca agattcctgc aaacctaccg ccaataaggg
     1621 gacggtttgt ctgccgagga taaaaaatgc cgtctgaaac ggagattccc gtttcagacg
     1681 gcattttcaa cagcaggaac gaatcaggca aatttcagtc tgtcgccgtc ggcttctacc
     1741 ctgatttcgc tttcgggcgc atagtttccg gcaagcaggg cttttgccag cgggttttcg
     1801 atttccgact ggatggcgcg tttgagcgga cgtgcgccgt aaatcgggtc gaaaccggct
     1861 ttggcgatga tgtccagagc ggcatcggaa acagtcaggc ggaggttttg tttttccaaa
     1921 cgtttttcca agcctttgag ctggattttc gcaatgttgc ggatattatc ctgatccagt
     1981 ccgtggaaca cgaccacttc gtcgatgcgg ttgatcattt caggacggaa atgctctttc
     2041 acatcctcca tcacaacttc tttcacggct tcgtagtcct gcgtgcccat ttgttggatg
     2101 tgctggctgc caatgttgga agtcatcaca atcacggtgt ttttaaaatc taccgtcctg
     2161 ccttgtccgt cggtcaaacg accgtcatcc aaaacttgca gcaggatatt gaacacgtcg
     2221 ggatgggctt tttccacttc gtccaaaaga atcacgctgt acggtttgcg gcggacttgc
     2281 tcagtcaggt agccgccttc ttcgtagccg acatagcccg gaggcgcgcc gattaagcgg
     2341 gcaacggcgt gtttttccat atattcggac atatcgatac gaatcagatg atcttcactg
     2401 tcaaacagga agcctgccaa tgccttacac aattcggtct tacccacgcc ggtcgggcct
     2461 aagaacagga agctgccgta aggtttgttc ggatcggcaa gaccggaacg gctgcggcgg
     2521 atagcgtcgg acacggcacg cacggcttca tcttgaccga ccacgcggcg gtgcaatact
     2581 tcttccatct tcagcagttt gtcgcgttcg ccttccatca ttttggacac gggaataccg
     2641 gtcatacggg aaacaacttc tgcgatttcc tccgcgccga cattattacg caagagtttg
     2701 tttgccggtt ttgtgctgtc cgtatctgcc cgttcggcgg ctgcacgctg tttttccaaa
     2761 tgctccaaat cttcatacat caattttgaa gccagtgcca aatcgccttg ccgttttgcc
     2821 tgttcgattt taattttgac ttcgtcaatt tgtttcttaa tattagcagc accgtctgaa
     2881 attgcttttt cggctttcca gatttcgtct aaatcggcgt attctttttg cagaccgttg
     2941 atttcctcgt ctatcagttc caaacgtttt ttgctggcat cgtctttttc tttttcaacg
     3001 tgtgcctttt ccatccgaag ctgaatcaga cggcggtcga ttttgtccat tgcttccggc
     3061 ttggtttctt tttccatctt gacacggctg gcggcttcat caatcaaatc aatcgctttg
     3121 tcgggcagga agcggtcggt gatgtagcgg tcgctcaact ccgctgcggc aacgatagca
     3181 gggtcggtaa tatcgatacc gtggtggatt tcataacgct cctgcaaacc gcgcaagatg
     3241 gcgatggtgt cttccacgct gggctcgccg accaatactt tttggaagcg gcgttcgagt
     3301 gccgcgtctt tttcgatgta ttggcggtat tcgtccaagg tggtcgcgcc gatacagtgc
     3361 agttcgcccc gcgccaaggc cggtttcagc atattgcccg cgtccatcgc gccgtcggtt
     3421 ttgcccgcgc cgaccaaagt atggatttca tcaatgaaaa tcagagtgtt gccgtcgtct
     3481 ttcgccaaat cgttcaacac gcctttcaag cgttcttcaa attcgccacg gtatttcgcg
     3541 ccggcaatca atgccgccaa gtccagaacc aaaaggcgtt tgttgcgcag ggattcgggt
     3601 acttcgccgt tgacgatgcg ttgcgccaag ccttcgacaa tggcagtttt acccacgccc
     3661 ggttcgccaa tcagcacggg gttgtttttg gtgcggcgtt gcaatacctg aattgcacgg
     3721 cggatttcgt cgtcacgacc gataacgggg tcgagcttac cgtcgcgggc gcgttgggtc
     3781 aaatcgagcg tatatttttt cagcgcatcg cgttggtctt cggcattggc atcgttcacg
     3841 ttttgtcctc ctcgtaccgc atcgattgcg gcattaatgt tttgttcggt cgcgccggct
     3901 tctttcaaaa ttttgccggt tgcatcgttc tgctgcacca aggcgagcag gaaaagttcg
     3961 ctggcgatat aggcatcgcc gcgtttggtt gctgctttgt ccatcaggtt caacaccgcc
     4021 tgcaattcgc ggctgggcat aatgtcgccg ccctgtccgg atactttcgg caggctgttc
     4081 aaatgctgct gcaaacgctg tttcacctgc ggcacgttca cgcccgcatg agccaagagc
     4141 gcggcagcac cgctgttttg gtcgtcaagc agggctttca gcacaaagcc cgcttccaga
     4201 tagctgccgt ccgcagccaa cgccaaactc tgagcttctg caagggcctg ttggaatttg
     4261 gcggttaatt tgtcgtatcg catttttgtt tccttttcaa aatgtccgct gtcgaagcct
     4321 atatgtgcat aattgtggat aactcaagtt ctgttttctg tttttctata tttaattcga
     4381 tatatcattg aatttaaagt atataaaaat gtataataat gtgtataact atatcttctt
     4441 aatatggaaa agtctgttgt cggctggatg taggtggcaa atcgggtata atcggcgcat
     4501 ctttttccct ttcagacggc attgatgccg caaggacatt tttatgagca aaaaacgagt
     4561 tctgaccggc gtaaccacca ccggcatccc gcatctgggc aactacgtcg gtgctatccg
     4621 ccccgccgtc cgcgcggcgc aaaaccctga taccgaatcc ttcctcttcc tcgccgatta
     4681 ccacggtatc atcaaatgcc acgagccgga gatgattcac caatccaccc aagccgttgc
     4741 cgccacttgg cttgcctgcg gactcgaccc cgagcgcacc accttctacc gccaaagcga
     4801 cattcccgaa gtgatggaat tgaactggat tctgacctgc atcaccgcca agggtttgat
     4861 gaaccgcgcc cacgcctaca aagccgccgt gcaggcaaat gcggaaaacg ggcaggaaga
     4921 ccccgatttc ggcgtggaaa tgggtttgtt cagttatccg attctgatga ctgccgatat
     4981 tctgatgttc aacgccaacg aagtccccgt cgggcgcgac caaatccaac acgtcgaaat
     5041 ggcgcgcgac atcgccggcc gcttcaatca ccgcttccga gaactcttca ccctgcccga
     5101 agtgaaaatc gacgaaaacg tcgaactctt ggtcggtttg gacggacgca aaatgtccaa
     5161 atcctacggc aacaccattc cgctttggga aaacgacaaa aaaacccaaa aatcggtcaa
     5221 caaaatcatc accaatatga aagagccggg cgagccgaaa cagcccgacg aaagcccatt
     5281 gtttgaaatc tacaaagcct tctccacgcc gtctgaaacg gtggaattta cgaaaatgct
     5341 tgccgacggc ctggcttggg gcgaggcaaa aaaacttttg gcggcgaaaa tcaacgccga
     5401 actcgccgaa ccgcgcgaac gctacaacga gctgaccgcc gacccttcgc aaatcgaaga
     5461 gattttacag gcaggtgcgc aaaaagcgcg taaagaagca cgcgaattgt tggacaaagt
     5521 acgcgatgcg gtcggcatcc gcccgttgaa ataaacccga tgccgtccga acctcttaaa
     5581 ggcttgccgg aacaaaacgt cgtccggctg accggcaagc atcccaacga cttggaagcc
     5641 gtcgtcggca aatgtatgga aaccgacgga aagggcgcgc cttcgggctg ggcggcaaac
     5701 ggcgtgtgcc ataccttgtt tgccaaactg gtgggcaata tcgccgaaga cggcggcaaa
     5761 ctgacggatt acctgatttc gcattccgcc ctgcaaccct atcaggcagg caaaagcggc
     5821 tatgccgccg tgcagaacgg acgctatgtg ctggaaatcg acagcgaagg ggcgttttat
     5881 ttccgccgcc gccattattg aggtattcgg acatcccgga atatatttgg gtttttcaaa
     5941 ccctgcggga aaaaagtccg caccgtcggg aaactcaaaa ggagggggat atgtgttaca
     6001 attttccgaa ctgttttcat aaaatagttt tcggacgtgt ttcaatatgg cattgatgcc
     6061 gccttattgt tcgagaaaaa aaacctttat atttaaatat aatgggtttt aactaaacgg
     6121 gaaaccgttt tctctccggc cgatgggcaa aatcagccga ttgatggaac acggtccgat
     6181 ttttaagcaa aaccttaatc aacaactcaa gaaggataag acatgaaaaa attattgatt
     6241 gccgcaatga tggcggctgc cttggcagct tgttcgcaag aagccaaaca ggaggttaag
     6301 gaagcggttc aagccgttga gtccgatgtt aaagacactg cggcttctgc cgccgagtct
     6361 gccgcttctg ccgtcgaaga agcgaaagac caagtcaaag atgctgcggc tgatgcaaag
     6421 gcaagtgccg aggaagctgt aactgaagcc aaagaagctg taactgaagc agctaaagat
     6481 actttgaaca aagctgccga cgcgactcag gaagcggcag acaaaatgaa agatgccgcc
     6541 aaataatttg ttgccttggc aaaaatgatg ggatgccgcc tgccggcaac ccaaacgaaa
     6601 ccgcctgaag attttcaggc ggtttttggg ttatgtgccc cttgtttttt acgcctgcat
     6661 gaccgttccg agcagatgtg ccgcgcatat cgggatgttg cggacaggca aatgccgtct
     6721 ggacagggtt tggacggcat ttgttccgta aggttcagac ggcctcgact ttaaacggca
     6781 tattgatttt ttgccattgg acgctttcat attccaatgc gtcggcaatc agggggtggc
     6841 gttccagcca ttccctgtca atacgcagga tgaagccgca gctttccgta tccgtgcgca
     6901 actgcatgtt tttcgggaaa gacaggtctt ggcgcgaacg gcagaacagt gcggcaaggc
     6961 gcagcgacaa aacggcatac cacaacattt cgttggtgcc gatgatgccg ctcatttttt
     7021 tcatatcgcc gcgatgaccg atgaccagtt gggcaagtat ggtctgttct ttgcgtgaga
     7081 aacccggcat atcggcgttt tcgaggatgt aggcggaatg cttgtgatag ccggtgtggg
     7141 cgatgtccaa accgatttcg tgcagcgcgg cggcgcgtcc gagatactgt tgccacaagg
     7201 caagctcttg aaccgtaaca tttttagcgt ggcagaggct gtccataaag gtttgcgcgg
     7261 tctcggcggt gcgtttcgcc tgattgaggc tgacgtggta gcggtgttgg aactcggcaa
     7321 ccgtttgtcc gcgcatatct tcgtttaaac cgcgcccgat caaatcgtaa aacacgccgt
     7381 cgcgcagggc ggcttcggtt acggtcatcc tgtcgagttt catttcttca aacgccgcca
     7441 tcatcacggc aagtccgccg gcaaaaactt cgatgcgttc cggtttcagg ttttcaaatt
     7501 tggccttttt gaccgaaccg gcttcgatga tgcgttcggc gagggcgcgc atgcctttgt
     7561 aggtaatgtc cgcctcttgg ggcatttcgg cggcaagcac gtcgcggatg gattttgccg
     7621 aacccgatgt gccgacggcg aaatcccaac cttcgcgcct catatttttg ctgatgcgct
     7681 ggatttcgtt gcgggcggcg gaaatggcag attggaagtc tttggcggtg attttgtttt
     7741 ggaagaagcg caggctgtag gttacgcagc ccaagggcag gctttcagta atgtcgggat
     7801 tcagcgtcga gccgatgaca aattctgtcg aaccgccgcc gatgtcgata accagcattt
     7861 tgccgccgcc cggggggagg gtgtggatca cgccggtata aatcagccgc gcctcttcgc
     7921 gcccggcgat gatttcgatg gggaaaccca gtgccgcttc ggccttggga aggaaatctg
     7981 cgatgttttt ggcgacgcgg aatgtattgg ttgccacggc gcgcacctgt tcagggcgga
     8041 agccgcgcag gcgttcgccg aattttgcca ggcagtccaa agcctgttct tgggaagcgg
     8101 cactcaggtt tttctgttcg tccagaccgg cggcgaagcg caccatctgt ttgaacgaat
     8161 cgataacttt taattgtccg ttgttgtttt cgcaaatctg gaggcggaaa ctgttggaac
     8221 ccaaatcgac ggaggcgagg acgtttgcgg gatgggtggt catggtggat accggtgggg
     8281 gaaaaacgca atgttactct gacggcgcag gcgttgacaa taaatgatgc ggcggttttt
     8341 gattctgccc acggatgttg ccgacggcat ttttgcgctt atttgaaatc cttttccacg
     8401 ctcatgaaaa tctgcgtgtt tttgcgtgtg taaaaacttt tcatattgct gtcgattttc
     8461 agatagcgga aattgagttg cggcgtaaag cccttccaag agattttgtc gtgccacaac
     8521 gacaggtttg cctgatattc gtggtctttg cgcgggaagc ggtacacaat ggtcccgggt
     8581 gcgtcaaaca tcctgcgggt atagcgcagg tttgcccgca gacccaagcc cgccgtcgaa
     8641 cgttttgact gcgccgacac gcaaaccctt gcggacggaa gcctgttccg cctctttcgt
     8701 catgttgtgc gaccagtccg caccgccgta aagcagccag tctttcggcg cggaatacat
     8761 cagcgtcgcg cccgccagcg gcatatggct gtcgtatcgg gcggcggtgc ggtcttcctg
     8821 ataacgcttc cacatattgc ccacgttcag cgtcagccgc cagcgttcgc tcaagcgtcg
     8881 ggagaaatcg gcattgaagc cgccgacgaa attgtatcgg ctgccgccta agaggttttg
     8941 cccgataaac ggcacgatgc cgaacgagcg cgttaccgaa cgatttttat agccgagcga
     9001 caggcgcagg cactgttcgc tgaaatcttt gttatcccaa taatgcacgc cgccgccgct
     9061 gatgccgccg tagaggaaat gatgcccgcc cgcattgatt tcgcgcgaca cgcctaagcc
     9121 ctagcgcaaa ccgtgtgcct tttgcggcag gctgtcggcg gttttcgtcc agcttctgcc
     9181 cgcaaattca atcgtttttt tggacgaagc gttgtttata g
//
LOCUS       DAXHPG010000072         9213 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXHPG010000072 DAXHPG010000000
VERSION     DAXHPG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.072"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(105..299)
                     /locus_tag="ACFM6F_001260"
     CDS             complement(105..299)
                     /locus_tag="ACFM6F_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC6164668.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(289..2370)
                     /locus_tag="ACFM6F_001261"
     CDS             complement(289..2370)
                     /locus_tag="ACFM6F_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC6164669.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEILAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2847..3227
                     /locus_tag="ACFM6F_001262"
     CDS             2847..3227
                     /locus_tag="ACFM6F_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC6164670.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3360..3854)
                     /locus_tag="ACFM6F_001263"
     CDS             complement(3360..3854)
                     /locus_tag="ACFM6F_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HFC6164671.1"
                     /translation="MIFKQNQNYWTVFYTNEEALIVQTCSGLGLTAIDHLYPPHILPL
                     DTDNETLGTTVLQALANSRTFVYDSPEDQDFFDTEKIRQRYEDWVAKLCGNLGYKTRR
                     ALFKNMMSVDIWLHNGCLKISPSRHVKLEAWDAIDADDVILSLDNSPEEIGAGLKLAL
                     SRCR"
     gene            3991..4155
                     /locus_tag="ACFM6F_001264"
     CDS             3991..4155
                     /locus_tag="ACFM6F_001264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164672.1"
                     /translation="MYKKVFFLNDRDCARAIFRHRPDVALPDRMLQTASEGFAVLEYE
                     DDNVREIDGI"
     gene            4188..5621
                     /locus_tag="ACFM6F_001265"
     CDS             4188..5621
                     /locus_tag="ACFM6F_001265"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC6164673.1"
                     /translation="MNEYYQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIIKPASLTPLSAYALASLAYEAGIPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDEFCRKLSEKVAELKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGASCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            5730..6518
                     /locus_tag="ACFM6F_001266"
     CDS             5730..6518
                     /locus_tag="ACFM6F_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC6164674.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVIEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            6886..8442
                     /locus_tag="ACFM6F_001267"
     CDS             6886..8442
                     /locus_tag="ACFM6F_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC6164675.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8459..9205
                     /gene="surE"
                     /locus_tag="ACFM6F_001268"
     CDS             8459..9205
                     /gene="surE"
                     /locus_tag="ACFM6F_001268"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC6164676.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKTPPQFPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAVFWHAD"
BASE COUNT         2157 a         2317 c         2613 g         2126 t
ORIGIN      
        1 gtactgtctg cggcttcgcc gccttgtcct gatttttgtt aatccactat attataaaaa
       61 ttccgtctga agtggcgttc agacggaatt tgtttcaagc aggcttaaca gcagcgtccg
      121 ccgtttgcgc cgcaacggcg cttgcggcag tagtcttgaa attgcagctt ggtcatcacg
      181 ggggcgttgg gattatgttt gcgctgctgt gcaacgtagt tttcataatc aggcacgcct
      241 gccatcaagt ttgccgtcag cttgatggtt ttccaccaag acgcgagctt atgcttcact
      301 ttgtgcctcc ggctgcttgc cgtcgcggta caccgccggg atttctttgg cggtcggcca
      361 gccgactttg cgtgctttga gggcggtacg caaaccgtac gcggcgacaa tcacgacaac
      421 cgacaagaag aggatggtca gaccggcatt aatcttgtcg ttgaagatga tttgcgccat
      481 ttcgccgatg tctttggcag gcgcaaggat ttcgttttta gccaatgcgt cgctgtattt
      541 gccggcgtgg gcaaggaagc tgatgcgcgg gtcgctgtgg aacagttttt gcaggccggc
      601 gtagcaggtt acgaacagta cgccgacggc gggaacgagt accacccaga cataacggtc
      661 gcgtttcatc ttaatcagca ccacggcgca cataatcaag gctacgcctg ccagcatttg
      721 gttggcgatg ccgaacaaag gccagagcga gttgatgccg cccaacgggt cggtcacgcc
      781 cgtgtagagg aagtagcccc acaatgccac ggcgaagaag gtcgcaatca ggttggcggg
      841 gatggagtcg gtgttgccga aaggtttgta gaagatgctg cccaagtctt gaatcataaa
      901 acgtgcgacg cgcgtaccgg catcgacggc ggtcaggatg aacaaggctt caaacaatag
      961 ggcgaagtga taccagaacg ccatcatcgc ctcgcccgga atcaggcggc tcataatgtg
     1021 cgccataccg actgcgaggg tgggcgcacc gcctgcacgg gaaaggatgg tgttttcgcc
     1081 cacttcctta gcggtgtgca acagggttgc cgcatcgaca gggaattgca gcttggtggt
     1141 aatcacttcg gcggcggtat tggcatccgt accgatcagg gcggcagggc tgttcatggc
     1201 gaagtacacg ccgggatcaa gcgatgcggc ggcggcaagc gccataatgg ctacgaaact
     1261 ttccatcaac ataccgccgt aaccaatcat acggacgtgg gtttcgtttt ccagcatttt
     1321 cggcgtggtg ccggaggaaa tcagcgcgtg gaagcccgaa accgcaccgc aggcgatggt
     1381 gatgaagagg aacgggaaca atgcgcctga gaataccgga cccgaaccgt cgataaagtg
     1441 ggttacggca ggcatttgca aagcgggatt gacgatgacg atgcccaaag ccaaggccgc
     1501 aatcgtaccg attttcagga aggtggagag atagtcgcgc ggagtgagca gcaaccatac
     1561 gggcaatacg gaggcgacaa agccgtaaat cataatcgcc caagtgagct ggatgccgtc
     1621 aaggtcgaac caatgtccga tggaactttt cgccacatct tcgccgtaaa ttaccgccag
     1681 catcagcaaa ataaagccga cgatggaaat ctcgccgatt ttgcccggac ggatatagcg
     1741 cgtgtaaata cccataaaca gggcaatcgg catagtggcg gcgatggtga acgtacccca
     1801 agggctgtga accagtgctt ttacgacaat caacgccaac accgccataa tgatgaccat
     1861 aatcatcaaa ataccgatgg aggcaatcac gccggggaca gtgccgagtt cctgtttcac
     1921 aatatcgccc aaagacttac catcgcggcg catagagacg aacaagacca tcatatcctg
     1981 taccgcgccg gcaaatacca cgccgaagat aatccacaaa gtaccgggca ggtaacccat
     2041 ttgcgccgcc aaaaccgggc cgaccaaggg gcccgcgccc gcaatcgcgg caaagtggtg
     2101 tccgaacaat acgcccttgt gcgtcggaac gtagtccaag ccgtcgttgt ggcgttctgc
     2161 cggagtcagg cggttaggat cgagccgcat cacgcggttg gcgatgtaga ggctgtaaaa
     2221 acggtaggcg atgcagtaaa cggatatggc ggcggtgacc atccataccg cgctgacctg
     2281 ttcgcctcgg ctgagggcca gagtggtaaa ggatgctaag ccgaccagta ccactatgcc
     2341 ccaaatgagg aaggttttga gtgatttcat cgaataaatc cttatctcac actgtcggaa
     2401 tatgcctgaa cggcgggaaa cggccgaacc atctgccggc acgggcacaa accggagtct
     2461 gaatgattga cgaagtatga aacagtgcgc ttgtcgggat ttgtgcctta tgcccgcatc
     2521 aaacagtgca gggatgcggg cgcacaaaac gttaaacgcc gaatatgatt ttaacgcaaa
     2581 ttagcacact gatagcggtt tttacttgga atttggaaaa aatttacatt cctccgtgcg
     2641 gacagggagg ttcagacggc atcgtcaggc aaaaggcggc gccggaagac gggtaaagaa
     2701 ggggcgcgca atccggatta ttgattcatc gcgggtaaat tctggttatc gggcttgtgt
     2761 gtttgcgcgt ccgtttatag tatggcgttg ccgcagcttg gaatcagggc ggttgtttca
     2821 tatcttattt ttattgggga gcttttatga atatcaggtt tttcgcgctg accgtaccgg
     2881 ttttgtcttt ggcggcctgt gccgtgccgg aggcgtatga tgacggcgga cgagggcata
     2941 tgccgcctgt tcaaaaccaa gccggcacgg acgattttcg ggcgttttcc tgcgagaacg
     3001 gtttgtctgt gcgggtccgc catttggaca gcggcaaagt cgcgttgcgg ctagacggca
     3061 ggcgtgccgt cctctcttcc gacgttgccg catccggaga acgctatacc gccgaacacg
     3121 gtttgttcgg aaacgcaacc gagtggcacc agaaaggcgg cgaagccttt ttcggcttta
     3181 ccgatgccta cggcaattcg gtcgaaactt cctgccgcgc ccgttaaacg gggcagcctg
     3241 tttgaaccgc cgtccggatt tttcgggcgg ttttttgtac cggttttgtt ttggccgtcg
     3301 gctggttccg gcggttttcg ggcgtggcgg attatttttc gttttgttcc gggtttattc
     3361 tatcggcagc ggctcaatgc caactttaaa cctgctccga tttcttcagg gctgttatcc
     3421 aatgataaaa tgacatcgtc tgcatcaatg gcatcccacg cttccagctt gacatggcgg
     3481 ctcgggctga ttttcaggca gccgttgtgc agccaaatat ccacgctcat catgttttta
     3541 aatagggcgc gtctggtttt atagcccaag ttcccgcata gcttggcaac ccaatcctca
     3601 tagcgttgcc gaattttttc ggtatcaaaa aaatcttggt cttctggact gtcataaacg
     3661 aaagtcctgc tgtttgccaa tgcttgcaag accgttgtgc ctaaagtttc attgtcggta
     3721 tccaatggca ggatatgagg gggatatagg tggtctattg ccgttaatcc caaacctgaa
     3781 catgtttgaa caatcagagc ttcttcattc gtataaaaaa ctgtccaata attttgattc
     3841 tgtttaaaaa tcattatttt atctccgtaa ttttgactgt aatgttttga cttttgccat
     3901 actctaccac acgttgcaac tgcaatcttt gctccttata tattgcaccc catcaaaggg
     3961 ctgcattact tttctttcta aagattacca ttgtataaaa aagtattttt cttaaacgac
     4021 agggattgcg cccgcgccat attcagacac cgcccagatg ttgcgctgcc cgatcggatg
     4081 cttcagacgg catcggaagg gtttgcagtt ttggaatatg aagatgataa tgtccgcgag
     4141 attgacggca tttgaaagcg gttattccgc ataccggagg acacgaaatg aacgaatatt
     4201 accaattaat caagcatccc gatatttccc tttccccgat ttcagacggc atcggggtcg
     4261 gcaatccggc aacgggcgaa attttggcgt atgtccgcaa tacgggttcg gacaaactga
     4321 aaaacctgat tcaaaaggcg gcggcagcgc aaaaattatg ggcggcgaaa actgctttgg
     4381 aacgcgccga catattgtgg cgttggtatt ttttagttaa agaaaacaaa gaagcactgg
     4441 cgcgcctgat gacgatggag cagggcaaaa gcctgaccga gtcgcgcggc gaaattgatt
     4501 atgcggcttc gtttgtgcgc tggtttgccg aagaggcgcg gcggattgac ggcgatgtgt
     4561 taacgagtgt aaaagcgtcg caaaaactgg ttgtgttgaa acagcctgtc ggcgttaccg
     4621 ccgcgattac gccgtggaac ttcccatctg caatgattgc gcgcaaggct gcgcctgctt
     4681 tggcggtggg ttgcgcgatg atcatcaaac ccgcatcgct cacgcctttg agtgcgtatg
     4741 ccttggcttc gttggcttac gaagcgggca taccgcagga tttgttgccc gtcgtcagcg
     4801 gccgcgcatc ggaaatcggc cgtgaatttg ccacgaaccc gattatccgc aaaatcagct
     4861 tcaccggttc gaccgaagtc ggcgcgaaaa tttttgccga cagcgcggcg gacattaaaa
     4921 aactcagttt ggaactgggc ggcaacgcgc cgtttatcgt gtttgacgat gccgatttgg
     4981 acaaagccgt cgaaggtgcg ctcgccagca agttccgcaa cagcggtcag acctgcgtct
     5041 gcaccaaccg cgtttacgcc caatccggca tttacgacga attttgtcgc aaattgagtg
     5101 aaaaagtagc cgagctcaaa ttgggcaacg gcttggagga tggcgtcaac caagggccgc
     5161 tgattgagga aaaagcggtg gagaaagtcg agcagcacat cgccgacgcg cttgctaaag
     5221 gtgcaagctg cctgaccggc ggcaaacgca gcgcgttggg cggaacgttt tttgagccga
     5281 ccgtcttaag cggcgtaacg gcgcaaatgg cggtggctcg cgaagaaacc ttcgggcctt
     5341 tgtgtccggt attccgtttt gaaaccgaag ccgaagtcat tgaggctgcg aacaatacgg
     5401 aatacggttt ggctgcttac ctttttaccg ctgacaccgc acgccagtgg cgcgtcggcg
     5461 aagcattgga atacggtatg gtcggcatca atacgggctt aatcagtaat gaagcggcac
     5521 cgttcggcgg cgtgaaacgc agcggtttgg gacgtgaagg cagcaaatac ggtgcggacg
     5581 aatatctgga attgaaatat ctgtgtatgg atgtcgggtg acggatgccg tctgaacggc
     5641 agggtttcag acggcgccgc attttaagca gtctctatct gttgtacaat gcgccctgtt
     5701 tttacggtta tttttgattt gaacaagata tgatggaaaa cggaaaaaca gtcccgagat
     5761 ggcgttttgc cttgaaaagt gcgggctggc atctcttaat cagcctgtcg gttgcagggc
     5821 tggcggcatt gctggttttt aaggtttggt atccttatcc ttatgccgag ctgacgggag
     5881 ggctgtcgct ttatcagctg gtggtggctg tcgatattgt atgtggtccg ctgctgactt
     5941 taattttggc aagcccgaag aaaaagacaa aggcacgcat ggtcgatttt tccatggtcg
     6001 gcatcatcca gctggcggct ttggtgtacg gtctgcacag cgtttctttg gcgcgccctg
     6061 tgatagaggc gtttgaaaaa gacaggatta atgttgtgac cgccgccgaa gtggcggttg
     6121 aagatttgca caaagcgccg gaagggctgc aaagcctgtc gtggttcggc atccgccgca
     6181 ttgcattgaa agaacctgag gatgcggatg agaagaacaa gacgctggat ttgtccctga
     6241 aaggtatcga gccgagtatg cgtcccgacc ggtggctgcc gtattccgac aaggaagcag
     6301 aaaaaatccg caaacatctg aaaccgctga aagtcttggc ggatgcgaga aaaacgacgg
     6361 ttgcggacat tctgaaacag gcaggtctcg ccgaagggga ggagctgtat tacctgccgt
     6421 ttaccagcag caggcagaaa gagtggatag tcattaccga taaagagggc aacaccaaag
     6481 gctacgcgcc gatagacggc ttcatcatca ccccttaagc gttgggactc cgtccgcact
     6541 cgaacatccg ttcttcgcgg cggtagaatc agactgtatt tgagaggggc gcaacccctc
     6601 aatcagggca acacataggg gaaggcttta tgtgccgtcc gtgtgttgaa acatttcaaa
     6661 ataagctcat gccgtccgaa catcctttca gacggtatgg cattttgcca ttgccccgat
     6721 aagctgttaa actatttaaa ttatttcgac cgaaggtaca cgcacatgaa agaccccgag
     6781 cagagcagca agcccgcccg ccgttttttg tgcgtaccgt ctgttcaggc agcttgagcg
     6841 cttcaggctg ccttttcgcg tttgaacttg aggaatacga aaatcatgga tttcagttgg
     6901 ttggcagaac cgcatacctg gataggtttt gccacgcttt tggtgttgga agtcgtattg
     6961 gggatagaca accttgtctt tgtggcgatt ttggcaaaca aggtccagcc cgcacggcgc
     7021 gaccgcgcac ggattatcgg gctggggctg gcagtcgtca tccgcatcat tatgcttgct
     7081 tttatggcgc acatcatcac gctgaccgag ccgctgttcc aaatcggcgg cctcgccgtt
     7141 tccggcaagg acatgattat gctcgcgggc ggtattttcc tgctttacaa agccaccacc
     7201 gaactgcatg aacgcctcga agggcacaac cgttttactg ttgccgacaa ccaaaaaaaa
     7261 cacgcgccgt tttggggcgt ggtcgcgcaa atcctgatac tggatgccgt gttttccatc
     7321 gattcggtca ttactgcggt ggcgatggtc gatcatatcg tcgtggcgat gggtgcggtc
     7381 gtcgtcgcaa tggctgtaat gatttctgcc agcaaactct tgaccgaatt tgtcgacaga
     7441 caccctaccg tcgtgatgct ctgccttggt tttttgttga tgatcggttt cagcctgatt
     7501 gccgaagcct tccatttcca cattcccaaa ggctacctct acgccgccat cggcttctcg
     7561 attttaatcg aactgtttaa ccagatttcc cagcgcaaca gccgcaaaaa cgactacatc
     7621 ggcagctcgt ggcgcaaacg gactgctgaa aacgttttgg gtatgatggg catacgcgaa
     7681 agcgtgcttg ccgacgcggg cggcgaatcc ggggacgacg cgcattttga agaaaacgaa
     7741 aaatcgatga tacgcagcgt gctgacgctt gccgaacgcc cgattatggg ggtgatgatc
     7801 ccacgccgcg acatcgaacg gctggacatt tcccaaagcc gcgaagaaca gtgtgcccaa
     7861 ctgcaaaaca cgccttacag ccgcctgctc gttgtcggaa aggcgggcgt ggacgaacct
     7921 ttgggctaca tcaacaaaaa agacctgctg tcccaactgc tggaaacagg cggtctcgac
     7981 attcagacgg cattgcgcca gccgctcgtc ctacccgacg gcacgaccgc cttgggcgcg
     8041 ctcgaactct tccgccaaag cagcgcggat tatgctttgg tggtggacga gttcggcgcg
     8101 gtattgggca tggtaaccat gaaagacctg ctcgaaacca tcgcaggcga gttccccgaa
     8161 gaatttgagc gcgaagaaga accagccgtt caggggaatc ccgatgaaag cctgacggtg
     8221 gaaggcgcgt tggaatatgt ggagctggca tcccaactca atctgccgca gcaggaggaa
     8281 gatgccgatt tccatacggt tgccgggctg attatggaag aattgcaaac catccccgat
     8341 gtcggcgatt ttgccgattt ccacggctgg cggtttgaag tggtcgaaaa agaagggcag
     8401 cgcatcgagc gggtcaaaat caccaaattg cccgaagaat aagcattcag gatagaaaat
     8461 gaacgttttg atttccaacg acgacggcta cctctccgaa ggtattgccg ttttggcgcg
     8521 cgttacggcg gaatttgcca acgtcagggt ggttgcgccc gaacgcgaca ggagcggggt
     8581 cagcaattcg ctgacgctgg aacgcccttt gcagttgaaa caggcgcaaa acgggttcta
     8641 ctatgtcaac ggcacgccga ccgactgcat ccacatcggg cagtctgtat tttcggattt
     8701 tcaggccgat tttgtctttt cgggcatcaa ccggggcgcg aatatggggg acgacacgct
     8761 ttattcgggg acggttgcgg cggcaaccga agcctacctt atgggcatac ccgctgtggc
     8821 gttttcctta aacgacgctt ccggacgcta ttgggcaacc gcagaaaagg cactgtggac
     8881 attgttggcg cattttttca aaaccccccc gcagttccct attttgtgga acatcaatat
     8941 ccccgccgtc gcgccggaag atgtgcgggg cattaaaatc gcccgtttgg gcaggaggca
     9001 tcacggtcag aacgtcatcc ccgcgcgcaa tccgcgcggc gaacagattt attggatagg
     9061 cccggtaggc agggtttccg acaaagaaga gggaacggat ttcggtgaat gcggcgcagg
     9121 tttcattacc gtaacgccgc tgcaaatcga cctgaccgcc tatccggaca tggcggaaac
     9181 agcggtgttc tggcatgcgg actgaccgtt tca
//
LOCUS       DAXHPG010000073         9149 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXHPG010000073 DAXHPG010000000
VERSION     DAXHPG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.073"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            249..471
                     /locus_tag="ACFM6F_001269"
                     /pseudo
     CDS             249..471
                     /locus_tag="ACFM6F_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(532..1485)
                     /locus_tag="ACFM6F_001270"
     CDS             complement(532..1485)
                     /locus_tag="ACFM6F_001270"
                     /inference="COORDINATES: protein motif:HMM:NF042780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6716 putative glycosyltransferase"
                     /protein_id="HFC6164677.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFRHASKGRHGEK
                     IYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            1868..2887
                     /gene="galE"
                     /locus_tag="ACFM6F_001271"
     CDS             1868..2887
                     /gene="galE"
                     /locus_tag="ACFM6F_001271"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC6164678.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFKIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLSV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRA
                     FEAASGLTIPFEIKPRRAGDLACFYADPSYTKAQIGWQTQRDLSQMMEDSWRWVSNHP
                     NGYDD"
     gene            3003..4070
                     /gene="rfbB"
                     /locus_tag="ACFM6F_001272"
     CDS             3003..4070
                     /gene="rfbB"
                     /locus_tag="ACFM6F_001272"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC6164679.1"
                     /translation="MRKILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESL
                     TDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNI
                     VGTFNLLEAARAYRQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPE
                     KPAGVARYEDLITFVQDRPGHDVRYAIDTAKISRELGWKPQETFESGLHKTVQWYLDN
                     KTWWQNVLNGSYRLERLGTGK"
     gene            4133..4999
                     /gene="rfbA"
                     /locus_tag="ACFM6F_001273"
     CDS             4133..4999
                     /gene="rfbA"
                     /locus_tag="ACFM6F_001273"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC6164680.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPTPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQMHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNQMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            5053..5610
                     /gene="rfbC"
                     /locus_tag="ACFM6F_001274"
     CDS             5053..5610
                     /gene="rfbC"
                     /locus_tag="ACFM6F_001274"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6164681.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDGWFKENIADCSF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFYVLSDTTELTYKCTDYYKPEAEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(5654..7672)
                     /locus_tag="ACFM6F_001275"
     CDS             complement(5654..7672)
                     /locus_tag="ACFM6F_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC6164682.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDIEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLMADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(7868..8989)
                     /gene="dnaJ"
                     /locus_tag="ACFM6F_001276"
     CDS             complement(7868..8989)
                     /gene="dnaJ"
                     /locus_tag="ACFM6F_001276"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC6164683.1"
                     /translation="MSNQDFYATLGVARTATDEEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYESCDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKAVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLNLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIIVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         2469 a         2369 c         2040 g         2271 t
ORIGIN      
        1 gaaggcgatg tgtttgaacc tgttgaaagc cgccaacagg ctaagtgcgc ccgctgccgc
       61 ctaaaaggtg ccccggatgc ctgattatcg ggtatccggg gaggattaag ggggtatttg
      121 ggtaaaatta ggaggtattt ggggcgaaaa cagctgaaaa cctgtgtttg ggtttcggct
      181 gtcgggaggg aaaggaattt tgcaaaggtc tcaggctgca tttctcaatg taaaacgcga
      241 tattttcacc cattattagg gataagtagc cagtggttta cactggctac tttcatagtt
      301 atgaagtact gggtctgagt gaattcaatt atttccgtat aaaacacagt agacatttta
      361 ctaatcggca aaattatatt agaagaattg agaactactg aagtcaggta agataccatt
      421 ttgcacaaat ttaacggcat ccctaaaaag cattttgggt ggtatttaaa ggagtacaaa
      481 atggttttaa ctacagtatg cttagaaatc tatagcctta cgcgctttat attaaatcac
      541 ctttctcaac catttagaat cataaaaaaa ctttttagga tccctaatta actttttaaa
      601 ttttcttaaa aaaatagttt tagttgattc tatactattc accgcagata aatattcttg
      661 gtaatcatgt tgcaatggca caacctgctt taatagctta ttaaactcat cagttgtaat
      721 caccttttta gtagcatagc aatcaagcca atccggatct gctatttttg gggaagtgaa
      781 atctatttga tcaaaataaa ctaaaacccc actacctaaa aaatgctgat ttgcatagga
      841 tttagaaaag cctaagtctg caacaacacc aacagggata cccttacatt cagcttcaaa
      901 aagcatagta gatgaataag ataaacaata ccccatttct tggaaggctt gcgcggtact
      961 ttttcgctct attgtcaaat tactcggcaa ctgaaactgc tttgccagct ctatatacga
     1021 atgtttatcc tgatgcacag taatatcttt atctgcaacc cttagcaaaa tagtaaattc
     1081 tttctcaggg tatttttcag ccaaggcaat caattttttt aatgtataaa ttctttcttc
     1141 ttttttaaat gggattttaa cttggtcaat aaagtaaatt ttctctccat gacgaccttt
     1201 tgatgcgtgg cgaaaaattg gataaccata aagtatattt tgacaactta atttatattg
     1261 tttcttatat tcctctgcaa ttctaaaatc atgcttatta ttatataaaa caatatcagc
     1321 ccccatacga gatagaatac ttgcctgatc accgaatact acacctggaa acaaggtaat
     1381 aatcaatggc cttgaagcaa tatttaattg cgcattctgc ttaaagaacc tttttaacaa
     1441 cccatttcca accgataaaa ttactgcgtc ataatattga tgcatattct taataaagta
     1501 atcatcaata tgaaaaaaaa aattgcttgg tctttatcta tccctgattc taataactgt
     1561 cgatttgaaa gaatgttttc tcgacttttg tggatataaa tatcaatttg agcatctttt
     1621 atctcttttg caacagcata gcccgagtta aggaacgagt cataactggc aattagtaat
     1681 acttttttca ctattttccc ttaattctac accatatgaa taaaattata gtattttacc
     1741 ttagatatca aatatactta accaaaaaaa tctgaagaaa tctactgcta cttcagggat
     1801 aattatttca ttaagatttt acaaccatta ttatcaatct atcaaagtat ttttagtatt
     1861 ttattctatg aaaaaaattc tcgttaccgg cggcaccggt tttatcggct cgcataccgt
     1921 tgtttctttg ctgaaaagcg gccatcaagt cgtgattttg gataacctat gcaattccag
     1981 catcaatatc ctgccacgct tgaaaacgat taccggccaa gagattccgt tttatcaagg
     2041 cgatatccgc gatcgtgaga ttttgcgccg tatttttgcg gaaaaccgca ttgattcggt
     2101 gattcatttt gccggcttga aagcggtggg tgaaagtgtg gccgagccga tgaaatatta
     2161 tgataataat gtttccggca gcttggtgtt ggcggaagaa atggcgcgtg ccggcgtatt
     2221 taagattgtg ttcagctcat cggctacggt ttatggcgat ccgggcaaag tgccttatac
     2281 cgaggatatg ccactgggcg ataccaccag cccttacggc acatcgaaat caatggttga
     2341 gcgcattctc actgatattc aaaaagccga tccgcgctgg agcgtgattt tgttgcgtta
     2401 tttcaatccg attggcgcgc acgaaagtgg tttgattggc gagcagccaa acggcatccc
     2461 gaataatttg ttgccttata tctgccaagt ggcggcaggc aaactgccgc agttgtcggt
     2521 tttcggcgat gactacccta ccccagacgg tacgggtatg cgcgactata tccatgtaat
     2581 ggatttggcg gaaggccatg tcgcggcaat gaaggcaaaa ggcggcgttg ccggcgtaca
     2641 tttgttcaac ttgggttcgg ggcgcgccta ttccgttttg gaaatcatcc gcgcatttga
     2701 agcggcttcg ggtttaacca ttccatttga aatcaaacca cgccgtgcgg gcgatttggc
     2761 gtgtttctat gctgaccctt cttatacgaa ggcacaaatc ggttggcaaa ctcagcgtga
     2821 tttaagccaa atgatggaag attcatggcg ctgggtaagt aatcatccga atggctacga
     2881 cgattaagtt gacctgatac aggccgtctg aaagagatgt tttcagacgg cctctttatc
     2941 tgaaaaacac acattctgtc tgctataatc tgtttatatt ttttggctat cctctgaaat
     3001 ttatgagaaa aatccttgtt accggcggcg cgggctttat cggttctgcc gttgtccgtc
     3061 atattatccg aaacacccaa gattccgtcg tcaacctcga caaactgacc tacgcgggca
     3121 atctcgaatc gctgaccgac attgccgata atccccgcta tgctttcgag caagtggaca
     3181 tttgcgaccg cgccgaactc gaccgcgtat tcgcacaaca ccggcccgat gccgtgatgc
     3241 acttggcggc ggaaagccat gtcgaccgct ctatcggttc ggcaggcgag tttatccaaa
     3301 ccaatatcgt cggcacattc aatctgcttg aagcagcccg cgcctaccgg caacaaatgc
     3361 cgtctgaaca gcacgaagcc ttccgtttcc accatatttc caccgatgaa gtctatggcg
     3421 atttaggcgg cacggacgat ttgtttaccg aaaccgcgcc ctacgcgccg tccagcccct
     3481 actctgcctc taaagcgtcc agcgaccacc tcgtccgcgc gtggttgcgt acttacggct
     3541 tgccgaccat tgtaaccaac tgctccaaca actacggtcc ttaccatttt ccggaaaaac
     3601 tcattccttt gatgattctg aacgcgcttg acggcaaacc gctgcctgtg tacggcgacg
     3661 gtatgcaaat ccgcgactgg ctgtttgtcg aagaccacgc gcgcgcactg tatcaggttg
     3721 ttaccgaagg tgttgtcggc gaaacctaca atatcggcgg ccacaatgaa aaagccaata
     3781 ttgaagtcgt caaaaccatc tgcgccctgc tggaagaact cgctcccgaa aaaccggccg
     3841 gtgtggcgcg ttatgaagat ttgattactt tcgtacaaga ccgccccggc catgacgtac
     3901 gctacgccat tgatacagcc aaaatcagta gggaactagg ctggaaaccg caggaaacct
     3961 tcgagtccgg cctccacaaa accgtgcaat ggtatctgga caacaaaacc tggtggcaaa
     4021 acgtattgaa cggcagctat cgtttggaac gcttaggtac aggcaaatag ttttcagacg
     4081 gcatcccgac gcaatgccgt ctgaaaaccc atcgcaaagg aagaaagaaa agatgaaagg
     4141 catcatactg gcaggcggca gcggcacgcg cctctacccc atcacgcgcg gcgtatccaa
     4201 acagctcctg cccgtgtacg acaaaccgat gatttattac cccttgtcgg ttttgatgct
     4261 ggcaggaatc cgcgatattt tggtgattac cgcgcctgaa gacaacgcct cttttaaacg
     4321 cctgcttggc gacggcagcg atttcggcat ttccatcagt tatgccgtgc aacccactcc
     4381 ggacggcttg gcacaggcat ttatcatcgg cgaagaattt atcggcaacg acaatgtttg
     4441 cttggttttg ggcgacaata ttttttacgg tcagtcgttt acgcaaacat tgaaacaggc
     4501 ggcagcgcaa atgcacggcg caaccgtgtt tgcttatcag gtcaaaaacc ccgaacgttt
     4561 cggcgtggtt gaatttaacg aaaacttccg cgccgtttcc atcgaagaaa aaccgcaacg
     4621 gcccaaatcc gattgggcgg taaccggctt gtatttctac gacaaccgcg ccgtcgagtt
     4681 cgccaaacag ctcaaaccgt ccgcacgcgg cgaattggaa atttccgacc tcaaccaaat
     4741 gtatttggaa gacggttcat tgtccgtcca aatattagga cgcggtttcg catggttgga
     4801 caccggcacg cacgaaagcc tgcacgaagc cgcttcattc gtccaaaccg tgcaaaatat
     4861 ccaaaacctg cacatcgcct gcctggaaga aatcgcttgg cgcaacggtt ggctttccga
     4921 tgaaaaactg gaagaattgg cgcgcccgat ggcgaaaaac caatacggcc aatatttgct
     4981 gcgcctgttg aaaaaataat gtttgaggcc gtctgaaact tttcagacgg cctttagatg
     5041 aaagataaaa agatgaacat cattgatacc gccattcctg acgtaaaact gcttgagccc
     5101 caagtcttcg gcgacgcgcg cggctttttt atggaaacct tccgcgacgg gtggttcaaa
     5161 gaaaatattg ccgactgttc ctttgttcag gaaaaccact ccaaatccgg caaaggcgta
     5221 ttgcgcggcc tgcactacca aaccgaaaac acacaaggca aactcgtacg cgtagttgtc
     5281 ggcgaagtgt tcgacgtggc cgtcgatatg cgcgaaggtt cgccgacttt cggcaaatgg
     5341 gcgggcgcaa ccctgtccgc gcaaaaccaa tgccagcttt ggatacccga aggcttcgcg
     5401 cacgggtttt acgttttgag cgacacgacc gaacttacct acaaatgcac ggactattac
     5461 aagcctgaag ccgaacaggt tttaatatgg aacgacccgg caatcggcat aggctggccg
     5521 cttcaaaccg cgccgctgct gtcgcccaaa gaccttgccg gcaaaacgtg ggcgcaagcc
     5581 gaaaagctcc gccttacact ttcccgataa aaaatgccgt ctgaacgttt cagacggcat
     5641 tttctccgac aggctaccta cccgccttca gtacgcgctg tgcaaagaaa aacatcccgg
     5701 taacgaagaa cgccaaaccc aaccaagaag cggcggcatc ccagttttgc agattcaacg
     5761 cgagcggcgc atccgagccg ccgttggtca cggagaaggc aataataaac gccataatca
     5821 caccgatcag gctttcaccg acaatcaggc cggcggagaa caaggttccg atgcgctcgg
     5881 cgtttttcag acggccttca cggttttccg cttttttacc gatgatgtgt ttcaacaccg
     5941 ccgccaacac cgcgcctgcc acgatgggca tattgacgga cggcggcaga taaataccca
     6001 taccgaccgc aaggacgggc agggcaagtt tgccgcctga tgattttttc aacaccaaat
     6061 cggcggcaat caacaccgcg ccaatcgcga tgccggtaaa gatatagacc cattcgaggt
     6121 tgtgggcgaa aatgcccgac gcgatggtcg tcatcaaagt cgcttgaggg gctgccaaag
     6181 cctgcgccgc gtccatacct tcgcgcggca ttgcgccggt aaagccgtag gcttcgtaaa
     6241 gcagttccaa cacgggtgaa ataaccagcg caccgacgat acagccgata atcagggcga
     6301 cttgctgccg ccaaggcgtg gctttgagca ggtagccggt tttcaggtct tgcaggttgt
     6361 cattggagat cgaagccacg cagattactg ccgagccgca aaataaagtc agtgccagca
     6421 aaaatttgcg gtttgcctca tccgccatca aaccgccgga ttcgcccacc agcagcaaaa
     6481 ccagcgaaat cacgacgatg gaaacgatgc ccacgccgga aatcgggctg gaagacgagc
     6541 cgaccaaacc cgccatataa ccgcaggcgg cggcgaccaa aaagccgatg acggaagcca
     6601 aaagcgtgca aacgaccacc aaaagccaag ccatgccgcc cgtaatgtgc gaatcgccga
     6661 taaagtggta aaacgacacg cctagaataa acatcataga caatacccag aaaatcatgg
     6721 ctttaggcga caaatcctgt tcagcgcgtt ctgtagcggg cgcaccgccg ccaaaactct
     6781 tgaacgacat cttcatgcct tccaccattg gcttgagcag catcaaaagc gtccaaaccg
     6841 ccgcgatacc tatggttccc gcgccgatga agcgtacttt ttccttccac agcttcatcg
     6901 caaacgccgc catttctata tcggaaggct gcgggatgtg cgatgaaaaa tacggcacgg
     6961 caatgcccca agcaatcgaa atgcccaaca gaatggcgat accgcccgtc agtccgacca
     7021 aatagcccgc gcccaacaat gccagtgaaa agcccatcgg cagttggaaa attgccgtac
     7081 cgcttttaaa ccaataactc gcgctatcgg caatcacacg cagacctccg gcgcaaaagc
     7141 tcatcaatcc cgccaacgca ccgccggccg ccagctcttt gatgccgctg ccgccctgac
     7201 ggttatcccc ttcttcatga ccgcccactt tcaaaatttc agcagccgcc acaccttccg
     7261 gataaggcaa atcgcttttc accaccatcg cgtaacgcag cggaatggtg aaaatcaccc
     7321 ccaaaatccc gccggcaata cataaaagcg tcgtctgcca gaacgggaaa ccgctccagt
     7381 agcccgccat cagcaaaccg ggcaggacga agatgatggt tgaaagcgta cccgcagccg
     7441 aggcttgggt ctgcaccatg ttgttttcca aaatattgct gcctttgaaa aactttaaaa
     7501 ccgccatcga aatcaccgcc gccggaatcg acgaggcaaa ggtcagcccg actttcaaac
     7561 cgaggtaaac gttggacgca gtaaaaatta cagtgatcaa tgcaccgagt atcatgcctc
     7621 ggagcgtcag ttcgcggtat tcttctaccg aaccggataa agatttattc attattcttc
     7681 ctttgacaac agattcccgc atattgttgg caatatgtca taatgtcaag ttttaaaaag
     7741 aacagttaaa aacagttatc caatcctatc tcgtactccc ttgaaaataa aaactatttt
     7801 caagcaaaca aaaacagtcg tatcagggag attccccgat acggccgcct gtttacaagc
     7861 ctcaaaatca atcaaacaaa tcgcgcagct tgtctaaaaa cgatttctta cgcggtgttt
     7921 gattttccaa cccggtagaa atccgttcaa attcttctaa aagctctttt tgacggtcgg
     7981 tcaaattaac aggtgtttcg acgataatat ggcagtacaa atcgccggtc gcgctgctgc
     8041 gtaaagattt gactccctta cccttcacgc gcatcctcct gccggtttgg gtttctttgg
     8101 ggacggtgag cttgactttg ccgtccaagg tcggcacttc caactctccg cccagagccg
     8161 ccgtggcaaa actgatcggc agttcgcaat gcaaattcaa gccgtcgcgt tggaaaatct
     8221 tatgcgcccg gatgcggacg gttacataca aatcgcctgc gggcgcgccg tgcatacccg
     8281 gcccgccttc gccgctcaaa cggatacgct gcccgtcatc gataccggcg ggaatattga
     8341 cttccaccgc cttgacggcc ttattccgcc ccgcgccacg gcatttgacg caaggttctt
     8401 taatgtgttt gccggcaccg tggcaggtcg gacaagtctg ctgcatacgg aaaatcgcct
     8461 gctggatatg caccgttccc gaacctttgc aagtcgggca ggtttccggg gatgtccccg
     8521 gtttcgcacc gctgccgttg cagacatcgc acgattcata agtcggaatg ttgatgcgtt
     8581 tcttcacgcc ttttgctgct tcttcaagcg tgatttcgat accgacttga acgtcctcgc
     8641 cctgataatc aggctgggcg cgccccgaac cgcctccaaa catttggctg aaaatatccc
     8701 caaagtcaaa accttgagca ccgccgaatc ctccaaaccc tccgaagccc ccctgtccgc
     8761 cgccttcaaa cgccgcatgg ccatactggt cgtacatagc gcgcttttct ttatcggaca
     8821 aagtttcata cgccttttgt acttctttaa acttctcttc cgcctcttta ttgtcaggat
     8881 tgcggtcggg atggtatttc atcgccaatt tacggtaggc ttttttaatt tcttcatcgg
     8941 tagctgttct tgccacacct agggtggcat aaaaatcttg attgctcatc ttttcatctg
     9001 attcaaaata aaatcgtcga taaaataagg gcaacaggac aaaacacaag gtttcccgaa
     9061 caaaaaccgc ctttcagacg gcatttcttc tgttgttcct ttaacaatgc gccgggtatc
     9121 ggcggcttta cccgtccgga aattgaaaa
//
LOCUS       DAXHPG010000074         8961 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXHPG010000074 DAXHPG010000000
VERSION     DAXHPG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.074"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            171..1094
                     /locus_tag="ACFM6F_001277"
     CDS             171..1094
                     /locus_tag="ACFM6F_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6164684.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(1159..2547)
                     /gene="fumC"
                     /locus_tag="ACFM6F_001278"
     CDS             complement(1159..2547)
                     /gene="fumC"
                     /locus_tag="ACFM6F_001278"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC6164685.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNDKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(2688..4667)
                     /gene="tkt"
                     /locus_tag="ACFM6F_001279"
     CDS             complement(2688..4667)
                     /gene="tkt"
                     /locus_tag="ACFM6F_001279"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC6164686.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DGYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQATVLPEGLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKEFGFTVDNVVDTVKSVL"
     gene            5194..6690
                     /gene="ccoG"
                     /locus_tag="ACFM6F_001280"
     CDS             5194..6690
                     /gene="ccoG"
                     /locus_tag="ACFM6F_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC6164687.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            6693..7241
                     /locus_tag="ACFM6F_001281"
     CDS             6693..7241
                     /locus_tag="ACFM6F_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC6164688.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(7583..8758)
                     /locus_tag="ACFM6F_001282"
     CDS             complement(7583..8758)
                     /locus_tag="ACFM6F_001282"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC6164689.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKYVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
BASE COUNT         1955 a         2182 c         2561 g         2263 t
ORIGIN      
        1 ttaaaattca ggcggtgttt tcaaatagcg cgaagcaata gccgagcttc agccggtttg
       61 tttgaaatca aagggttgga atcaaacgcc aaacgcttga accatcattg cccgatgcgg
      121 caaaccggca tcgtccggca atacggttgt ttaaaaatag gaaaatcagg atggaaaacc
      181 aaaggccgct cctaggcttc gcgttggcac ttttggcggc gatgacgtgg ggaacgctgc
      241 cgattgccgt gcggcaggta ttgaagtttg tcgatgcgcc gacgctggtg tgggtgcgtt
      301 ttaccgtggc ggcggcggta ttgtttgttt tgctggcact gggcgggcgg ctgccgaagc
      361 ggcgggattt ttcttggtgc tcattcaggc tgctgctgct cggcgtggcg ggcatttcgg
      421 caaactttgt gctgattgcc caagggctgc attatatttc gccgaccacg acgcaggttt
      481 tgtggcagat ttcgccgttt acgatgattg ttgtcggtgt gttggtgttt aaagaccgga
      541 tgactgccgc tcagaaaatc ggcttggttt tgctgcttgc cggtttgctt atgtttttta
      601 acgataaatt cggcgagttg tcgggtttgg gcgcgtatgc gaagggcgtg ttgctgtgtg
      661 cggcaggcag tatggcatgg gtgtgttatg ccgtggcgca aaagctgctg tcggcgcaat
      721 tcgggccgca acagattctg ctgttgattt atgcggcaag tgccgccgtg ttcctgccgt
      781 ttgccgaacc ggcacacatc ggaagtttgg acggtacgtt ggcgtgggtt tgttttgcgt
      841 attgctgctt gaatacgtta atcggttacg gctcgttcgg cgaggcgttg aaacattggg
      901 aggcttccaa agtcagcgcg gtaacaacct tgctccccgt gtttaccgta atattttctt
      961 tgctcgggca ttatgtgatg cctgatactt ttgccgcgcc ggatatgaac ggtttgggtt
     1021 atgttggcgc actggtcgtg gtcgggggtg cggttacggc ggcggtgggg gacaggctgt
     1081 tcaaacgccg ctagttcgca ggcaacggaa aatgccgtct gaacgaggtt tcagacggca
     1141 ttttatttga gggaaagatt agcgcggatg aaccatatcg gcaggaacga ccagttcgtc
     1201 aaattcttcg cccgtcagca agcccaactc aacggcggtt tcgcgcagcg atttgtcgtt
     1261 tttgtaggcg gttttggcga ctttggcggc gttttcgtaa ccgattttgc ggtttaacgc
     1321 ggtaacgagc atcagggaat ggtgcaggaa atagtcgatt ttttccggta cgggttcgat
     1381 gccgacggcg cagtgttcgt tgaagctgtt gcacgcgtcg cccaagaggc ggatggattg
     1441 caagaggttg taggcgataa cgggcatata gacgttcagc tcgaaattgc ccgacgcgcc
     1501 cgccataccg atggtaacgt cgttgccgaa cacttggcag cacaccatcg tcattgcttc
     1561 gcattgggtc gggttgactt tgcccggcat gatggacgaa cccggctcgt tttcggggat
     1621 tttgatttcg cccaaaccgc aacgcgggcc gcttgccagc cagcggatgt cgttggcgat
     1681 tttattcagg cttgccgcca gcgttttcaa tgcgcccgaa gcggcaacgg cggcatcgcg
     1741 tccgcccaag gcttcaaatt tgttcggcgc gctgacaaac ggcaagccag acagttcggc
     1801 gagtttagca gcagcttttt cggcgtattc gggatggctg ttcaaacccg tgccgaccgc
     1861 cgtaccgccc aaagcaagtt catacaagtc tttaagcgca tcgttcagac ggcctaaacc
     1921 gtgatcaagc tgggaaacgt agccggaaaa ttcctgtccc aaagtcagcg gcgtcgcgtc
     1981 ttgcaagtgg gtgcggccga ttttaacaat gggggcgaaa gcttgggctt ttttgtccaa
     2041 ggtatcgcgc agggctttta cggcggggat gaggtggcgg ttgatttcaa tcgcggcggc
     2101 aacgtggata gcggtcggga atgcgtcgtt ggtcgattgc gcgtggttca catggtcgtt
     2161 gggatggacg ggctgataag ccgccaaacc cgtaccggcg atttcgttgg cgcggtttgc
     2221 cagcacttcg ttcatgttca tattggactg cgtgccggag ccggtctgcc aaaccactaa
     2281 aggaaactgt ccgtcaagcc tgccgttcaa cacatcatcc gccgcctgcg taatcaaatc
     2341 cgcctgttcc ggcttaatcc tgccgaggga aacattggtc gcggcagcgg cttttttcac
     2401 caacgccaaa gcataaatca gcggctgcgg cagggtttcg ccgccgattt tgaaattgtt
     2461 gcggctgcgc tgggtctgcg cgccccaata ggcttcggac gggacttcga cattgcccat
     2521 ggtgtcgtgt tcggtgcgag tgttcatacg tttctccttt tgaaatgtga ataagagtga
     2581 ttcgcaaata ttataatgga gattggtgga atgaggaagc attaaggagg gagggtgtga
     2641 aaatgccgtc tgaaaagcgt tcagacggca tttgtttagg tgtgggttta cagcacggat
     2701 ttcactgtat cgaccacatt gtccacggta aagccgaatt ctttgaagag taagtcggca
     2761 ggggcggatt cgccgaagcg gttgatgccg acgactgcgc cgttcaaacc gacgtatttg
     2821 taccagccgt cggcgtgtcc ggcttctacg gcgatgcgcg gcagaccttc gggcaggacg
     2881 gtggcttgat aggcggcgtc ttggcggtcg aatacgttgg tggacggcat ggaaacgacg
     2941 cgtacggcga tgttttgcgc ggcgagggct ttttgcgctt ccaaagccag ctcgacttcg
     3001 gaaccggtgg caatgatgac ggcttgcgcg ttgccttggg cttcgctgat gatgtagccg
     3061 ccgcgtttga tgtcgttcag ttgttgttcg ctgcgcgctt ggaatttcag gttttgacgg
     3121 ctgaaaatca ggcaggacgg atgatcggcg gctttgacgg cttctgccca agccaccaga
     3181 gattcggcgg tgtcgcacgg acgccatacg tccatgttcg gaatcaggcg cagggtggcg
     3241 gtttgctcaa tcggctggtg ggtcgggccg tcttcgccca aaccgatgga atcgtgggtg
     3301 aacacaaata cagggttgat tttcatcagt gccgccatgc gcaaggcgtt gcgttcgtat
     3361 tcgctaaaca tcaggaaggt cgcgccaaag ggttttacgc cgccgtgcag ggcaagaccg
     3421 ttcataatcg cacccatacc gaactcgcgc acgccgtagt ggatgtagtt gccgcctttg
     3481 tcgcgggtaa cggagacgct gtttgaccag tcggtcaggt tggacggggt caggtcggcc
     3541 gaaccgccca ccaattcagg caactctttt gccaagattt cgatgctgtt ttggctggct
     3601 ttgcgggtag cgatggtttc ggctttggcg cacacttctt tcaatgccgt ctgaacgtac
     3661 ccgtcaaagt tttccggcag ctttttatcc atacggcgca caaattctgc ggcttcggca
     3721 ggatatttgg cttgatattg cgcgaacagt tcgttccagt cggcttccag tttcgcgcct
     3781 tgttctttgg cattccacgc atcgtaaatt tcttgcggga tttcaaaggc ggggtaagtc
     3841 cagcccaaat gtttgcgcgt ggcttcgatt tcgtccgcgc ccaaaggtgc gccgtgggtt
     3901 ttgtggctgc cttctttgtt ggcactgcct ttgccgatta aggttttgca gcagatgatg
     3961 gacggtttgc cggtttcggc acgtgcggct tcgatggcgg cttgaatggc ggcggtgtca
     4021 tgaccgttta cgttaggaac gacgtgccag ccgtagcttt caaagcgttg cgggatgttt
     4081 tcggtaaacc agccgtccac tttaccatca atggaaatat tgttatcgtc atacaaaaca
     4141 atcagtttgc ccaagcctaa agtgccggcg agtgagcagg cttcgtgcga tacgccttcc
     4201 atcaggcagc cgtcgcccat gaagacgtag gtgtaatgat cgacgatgtt caaaccgtct
     4261 ttattaaatt cggcggcaag gattttttct gccaatgcca tacccaccgc gttggcaatc
     4321 ccttgcccca acgggccggt cgtggtttcc acgccgtcgg tgtagccgta ttcgggatgg
     4381 ccgggggttt tgctgtgcag ttggcggaag tttttcaagt cttcaatgct taggttgtag
     4441 ccggtcaggt gcagcaggct gtacaacagc atagacgcgt ggccgttgga gaggacgaag
     4501 cggtcgcggt tgtagaattt ggggttggcg ggattgtgat tgaggaattt cgtccacaat
     4561 gtttccgcca tttccgccat acccataggc gcgccggggt ggccggaatt ggctttttga
     4621 acggcatcgg ccgagaggaa gcggattgcg tttgccagtt gagacatttt gtattttcct
     4681 tgctggtgtt tcggataagt ggataatcgg aaggcgttga ttatcgcccg attcgcttat
     4741 gctttcaaga aaagggcgga cgcgtgggaa ggcggcggca ggggacaggc ggggaaggat
     4801 tttcgatttg cgggcgaagc ctgccattat tccttttgaa ataaaatgtt atagattgtg
     4861 tgccggattg tcgatagcgt ttgtttatga gcttgcgccg tcggttctgc cgatatgggg
     4921 gtgtcggttt ttttagtctt ttttttaacc gtattcggat tttgttcggg cggtaaggta
     4981 aaatccaggc gttttgatgc ggatgggaaa tgtatccgcc ccatacatcc ggacggcgca
     5041 taaagttgta caatagcgga aatatatttt gggaaaaacg gattttctca aaacttgaaa
     5101 cacaacgcct taaaaaacaa gaaaaatgac gtctgaaaag caaacggcgg cggtaacggg
     5161 caaaccatca atcaaaaatc taaggaatgc agaatgacca cggaaaacca agccggcagt
     5221 ccggcatccg gaatcggcac gtccgaacaa acaaaagccg cgccgaaagc aaaaaaaaca
     5281 ttcgatccgc gagccagcgt gattcaaatc catcccgaag gcgaacgcat ccatccgaaa
     5341 aaggcggaag gacggttcgc caaactgcgt atcgccgccg tattggcgac gcagtttgtg
     5401 ttttacgtca ttccgtggtt caactggagc ggcaggcagg ccgtcgtttt caatatcccc
     5461 gaacggcatt tcttcatttt cggattgtcg ttgggggtgg gcgatttgat ttaccttgcc
     5521 ttgctgctga tgatttgcgc cttcgggctg ttttggtgga cgacgattgc cgggcgactg
     5581 tggtgcggct attcctgccc gcaaacggtt tacaccgaaa ttatgctgtg gatcgacaac
     5641 ctggtcgaag gcgatagaaa caaacgcctg aagctggaaa aatcgccgtg gaatttcact
     5701 aaaatccgca tcaaagccac caaatacctg ttgattttcc ttgtctgcgc gtggacgggc
     5761 atcacgtttg caggctggtt tgtccctatc cgccagttcg ttcccgattt attcactgga
     5821 gcagcaggcg gcggcgcgat gtttgccaca gcgttttacg gctttatgac cttcttcttc
     5881 gcacatatta tgcgcgaaaa agtatgcctg catatgtgtc cgtatgcacg tttccaaagc
     5941 gcgatgttcg acaaggacac gctgattgtt tcttatgacg cggaacgcgg cgaaccgcgc
     6001 ggagcccgca agaaaacggt caacaaggaa gaggcgggtt tgggcgactg catcaactgt
     6061 gcgatgtgcg tccaagtctg ccctgtcggc atcgacatcc gcaacggtct gcaataccaa
     6121 tgcatcggct gcgccgcctg tatcgacgcg tgcgatgaga ttatggacaa aatgggctat
     6181 ccgcgcggat taatccgtta tacgaccgaa agcgcgctgg aacacgaata ttctgaaaaa
     6241 gacattaaaa aacggctgct cagaccgcgc gtggcaggtt acggcgcggt gttggcgtta
     6301 gttatcattg ccttcctggc cggtttgtcc acgcgcaaaa tggtcgaggt cgatattttg
     6361 aaagaccgtg gcgtactggt gcgcgaaaac gccaaaggct ggctggaaaa cgcatacagc
     6421 ctgcgtatca tcaacaaaag tgaaaaagaa cagctcatta ccgcaagtgt caaaggcttt
     6481 gacgaaatcg ccctgaccgg gctgcccgaa ggcggtatca aggttgcccc gcgcgaaacg
     6541 gtaacccttc ccgtccaagt gtccaccatt ccggaatacg cggacaaagg cagccaccct
     6601 atcgaattta ccttccaata ccgcgaaagc ggcgcgcccg acggcaagcc ggtcgtcttg
     6661 gaagaagatg caacctttat cggagaataa ccgtgtctca aaacactcca atcaaacctt
     6721 ggtacaaaca cgtctggccg tggatcttga tggcggggcc gatttttgtc gtcatcgcca
     6781 gcgtcgctat gttttttgtc gcacagcagc acgcgacaga tttggttacg gacgattatt
     6841 ataaagacgg caaacatatc gacatccagc ttcatcggga tgaagaagcc gtcagacggc
     6901 atatcggggt gcaggttctc atttcccccg atatgaatgc ggcaaaagtg tttgtcggcg
     6961 gcgagtttga cggcaaacag cctttgaacc tgctgctgat gcacccgacc cgcaaggcgg
     7021 acgatcaaac cgtcgccctc aagcccgtcg gcagcgcgca gaacggcagg gcggaatatg
     7081 aggcggtgtt caaaaccctt tcgccgacca accactggta tgtgcgcgtg gaggacgcgg
     7141 caggcgtgtg gcgcgtcgag aacaaatgga ttaccagcca aggcaatgcg gtggatctga
     7201 ccccgatgga taaacttttc aataatgccg gaagcaaata aaaaagccgt gttttcattg
     7261 cattggtttg ccagatgcaa tgtcgggcag gcattgtttg attattcggc gtgatggtct
     7321 gatttatcgg acgaaaataa atgtatctca tcccgtttat tttttaacac tattccagaa
     7381 cgcagcctga aaaatgccgt ctgaagggct tcagacggca tttgtgttgt atgcccgaca
     7441 caatgcggaa ggcgttttgt ttacataaaa acgtttttat ttctctccgt tttttcgaca
     7501 ctattccgaa acgcagcctg aaaaatgccg tctgaaagcc cttcagacgg cattttgttt
     7561 gcaaatcaaa tcctacctga tgtcagcgtc cgagcgttgc aacaggttcg ctccccgtca
     7621 gcagatattg tatcgtctcg cggacggtgc ggatggcatt accgaagggc agcgggtctt
     7681 tacctctgaa gaacaggcct ttatctactt ctccacgaaa tgcggcagca agctggatat
     7741 caatgcagaa ttgtccggct ttggaaagcc cgtcgcgcag accgcaactg gttaggcagt
     7801 ttaaaccttg ggtacagcgg cgcgggtcgg ctttggcgtt tgtctgaagt ttgctttcac
     7861 gcttgatgta gctgtctagg aatttggtgc ggacaccgcg cgccggcaaa ccggcaacag
     7921 acataaattc gactactttt tcagtttccg caccggcgag cgtttttttg aagttaaggt
     7981 gtgcatctcc ttcttcggta acggcaaaag ccgtaccgat ttgaacggcg gatgctcccc
     8041 agttctttag ggcggttttg actttttcaa aatttgccat gcctcccgca agaataagcg
     8101 ggattttttc gctttccagc cctaaacttt tgaaaacttc aaacgtttcc tcaatcacgc
     8161 gtttgaagtc gaacttggca tcgtttacgc cttcaacggt tgatgcaccc aaatgtccgg
     8221 ccgcgtgggc aggatgttcg actacaatcg catcgggcaa tatgcctttt ttcatccaac
     8281 gtttcaaaac gatattaata ccgcgcgatt cggacagaat cggcagcagc gcgacatatt
     8341 tatgatagcc ctcggtcatt tccggcaggt ctaaaggcag accggcaccc attacaaccg
     8401 catccgcccc tgattcgcaa gcctggcgga catatgcggc gtggtctttg accgccttca
     8461 tcacgttgac cgcaatcagc ccttttccct ctgaagcact tttggctttt tggatttccc
     8521 tgtccaatgc ggtacagttc aaagacgtat atttctcttc actcggattg atttgggatt
     8581 cggcgagtag gtcttcgtga aggtggcgca aatccacact ggcaatcgtt ccgataccgt
     8641 tttcacgtgc caccgcgctg gataaacccg atgcggaaac accgaccccc ataccgcctt
     8701 gcacgatggg ggtaagggat tttccacgaa taaccaaagg gtcaaaaata ttctgcatca
     8761 gtttctccga gtgtacgaat cagtcaggct catgaaaaat ggttctaact gttttagaac
     8821 tattgctcaa atttggtatt atactctaaa tatcgcggcg gtaaacaaga aagcagccgg
     8881 agcttcgggc atattctgtt acgcctgttt caatcgggag agaaaatatt atagtgaatt
     8941 aacaaaaatc aggacaaggc g
//
LOCUS       DAXHPG010000075         8927 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXHPG010000075 DAXHPG010000000
VERSION     DAXHPG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.075"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            165..944
                     /gene="pgeF"
                     /locus_tag="ACFM6F_001283"
     CDS             165..944
                     /gene="pgeF"
                     /locus_tag="ACFM6F_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC6164690.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAESLGGTPNADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            996..1475
                     /gene="lptE"
                     /locus_tag="ACFM6F_001284"
     CDS             996..1475
                     /gene="lptE"
                     /locus_tag="ACFM6F_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC6164691.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1477..2475
                     /gene="holA"
                     /locus_tag="ACFM6F_001285"
     CDS             1477..2475
                     /gene="holA"
                     /locus_tag="ACFM6F_001285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC6164692.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEINKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGEKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            2596..3015
                     /locus_tag="ACFM6F_001286"
     CDS             2596..3015
                     /locus_tag="ACFM6F_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164693.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3090..3605
                     /locus_tag="ACFM6F_001287"
     CDS             3090..3605
                     /locus_tag="ACFM6F_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6164694.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3689..4573)
                     /locus_tag="ACFM6F_001288"
     CDS             complement(3689..4573)
                     /locus_tag="ACFM6F_001288"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC6164695.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGNQSLELNRDLVGQLAEINKDLR
                     KHYDLAKLGVADILRFPGVLASQRENADALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5057..5920)
                     /gene="rpoH"
                     /locus_tag="ACFM6F_001289"
     CDS             complement(5057..5920)
                     /gene="rpoH"
                     /locus_tag="ACFM6F_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC6164696.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYKPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(6184..7722)
                     /gene="lnt"
                     /locus_tag="ACFM6F_001290"
     CDS             complement(6184..7722)
                     /gene="lnt"
                     /locus_tag="ACFM6F_001290"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC6164697.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(7791..8517)
                     /locus_tag="ACFM6F_001291"
                     /pseudo
     CDS             complement(7791..8517)
                     /locus_tag="ACFM6F_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            8711..>8927
                     /locus_tag="ACFM6F_001292"
     CDS             8711..>8927
                     /locus_tag="ACFM6F_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HFC6164698.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSACLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNY"
BASE COUNT         1976 a         2228 c         2612 g         2111 t
ORIGIN      
        1 taagggcatc attcccgcac cggcaagaat ccgaacccga acgtttgaaa acaatcccga
       61 atctccgaat tcccgcctgt gcgggaatga cgaaaaaaca aacatccatt tgccccgaag
      121 gcagttaatc aaccctttcc gccacacacc tattccaata tccaatgaaa accatcacag
      181 aaaccctaaa tctcgccccg aaaggcaaaa acttcctgac cgccgattgg cccgcgcccg
      241 ccaatgtgaa aaccctgatt accacgcgca acggcggcgt gagccaaggt gcgtatcaga
      301 gtttgaacct cggtacgcac gtcggcgaca atcccgaagc cgtgcgccgc aaccgcgaaa
      361 tcgtgcaaca gcaggtcgga ttgcccgttg cctacctcaa tcaaatccac agcaccgtcg
      421 tcgtcaacgc tgcagaatcg ttaggcggta cacccaatgc ggacgcttcc gtggacgaca
      481 cgggtaaagc cgtctgtgct gtgatgaccg cagactgtct gcctgttttg ttttgcgaca
      541 gggcaggtac ggcggttgcc gccgcacacg cgggctggcg cggtttggcg ggcggcgtat
      601 tgcaaaacac cattgctgcg atgaaggttc cgcctgtcga aatgatggcg tatctcggcc
      661 ctgccatcag tgcggatgcg tttgaagtcg gacaggacgt gtttgatgcg ttctgcacgc
      721 ccatgcccga agccgccgcc gcatttgaag gcataggcgg cggcaaattc cttgccgacc
      781 tttacgcgct cgcccgcctg attctgaagc gtgaaggcgt gggcggcgta tatggcggca
      841 cgcattgtac ggttttggaa cgggacactt tcttttccta ccgccgcgac ggagcgacag
      901 ggcgtatggc gagcctgatt tggctggacg gcaatgccgt ctgaacacgc cgctgatata
      961 atctaccgac tttgtgcttt tgagaaaggc aagccatgaa caaactgttt cttactgccg
     1021 cagtgctgat gctgggcgcg tgcggtttcc acctgaaagg tgcagacggc atttctccgc
     1081 cgctgaccta ccggagctgg cacatcgaag gcggacaggc attgcagttt cctttggaaa
     1141 ccgcgctgta tcaggcttcg ggcagggtgg acgatgctgc cggcgcgcag atgaccctgc
     1201 gtatagacag cgtttcccaa aacaaggaaa cctacaccgt tacccgtgcg gcagtcatca
     1261 acgaatatct tttgatattg acggttgaag cgcaggtatt gaaacgcggc gagccggtcg
     1321 gcaaaccgat gaccgtgtcc gtccgccgcg tccttgctta tgccgacaac gagatcttgg
     1381 gcaaacagga agaggaagcg gcattgtggg cggaaatgcg gcaggatgcc gccgaacaga
     1441 ttgtccgccg cctgaccttt ctgaaggcgg aatgacgtgg cggcacatat cggacgcatt
     1501 gatacggacg cgcctttgaa acccctgtac gtcatccacg gcgaggaaga actgttgcgt
     1561 atcgaggcat tggacgcatt gagggcggcg gcgaagaaac aaggttacct taatcgggaa
     1621 gtttatacgg cagacaatgc tttcgattgg aacgagctgc tgcaaaccgc aggcagtgcg
     1681 ggtctgtttg ccgatttgaa gctgttggaa ctgcatatcc ctaacggcaa gcccggcaaa
     1741 accggcggcg aggcgttgca ggattttgcc gcccgcctgc cggaagatac ggtaacgctg
     1801 gttttactgc ccaaactgga gaaaacccag ctccagtcca aatggtttgc cgcattggcg
     1861 gcaaaggggg aagtgtggga agccaaaccg gtcggcgcgg cggctttgcc ccaatggata
     1921 cgcggacggc tggacaaaat cggtttgggt atcggggcag acgcattggc actgtttgct
     1981 gagcgcgtgg aaggcaatct gttggcggca cgtcaggaaa tcaacaagct cgggctgctg
     2041 tatccgaaag ggcataccgt caatatcgat gaggcgcaaa ccgccgttgc caacgtcgcc
     2101 cgcttcgacg cgttccaact ggcaggcgcg tggatgaagg gcgatgtccc gcgcgtatgc
     2161 aggctcttgg acggattgcg ggaagaaggc gaagaaccgg tactgcttct gtgggcggtt
     2221 gccgaagacg tgcggacgct gatccggctt gccgccgccc tgaagcaggg gcagagcatc
     2281 caatccgtcc gcaacagtct cagactttgg ggtgaaaagc agacgctcgc gccgcttgcg
     2341 gtcaagcgga tttccgtcgt ccgcctgctt gacgcgctca aaacctgcgc ccaaatcgac
     2401 cgaatcatca aaggtgcgga agacggcgac gcatggacgg tattcaaacg gcttgtcgtg
     2461 tcgctggcgg aataaagcgg taatccctaa aatccgaaaa tactgtaaaa taccgttaat
     2521 cctgaaaagt attcaccaat ccgtccgaaa acatttcaga cggcacgacc acctcaataa
     2581 aggaacatca accctatgga caataagacc aaactgcgct tgggcggcct gattttactg
     2641 accaccgccg ttttaagcct cattatcgta ttgattgtcg attcctggcc gcttgccatc
     2701 ctgcttgccg ccgtcatcgt cgccgccgct gcgggcggct ttgtttggac atcccgccga
     2761 cagcaacgcc agtttatcga acgcctgaaa aaattcgaca tcgatcccga aaaaggcaga
     2821 atcaacgagg caaacctgcg ccgtatgtac cacagcggcg gacaacacca gaaagatgcg
     2881 attaccctga tctgcctgtc gcaaaaatgt tcggtggacg aggcgcacgc tatgttcaaa
     2941 aaacgcccga cacgtcagga aatcaatcaa atggcggcaa aacagtcgcg cggtcagaaa
     3001 cgtccgcacc gttaaccaca tgagacatat ctgcatgaat gccgtctgaa gcctgttggc
     3061 gtttcagacg gcatattccg attgaaaaga tgatgatact gaaaaccgcc ccgctcaaac
     3121 gccgctttgc cgccatgctg tacgaaatgc tgctggtcgg tgcggcaacc tgtttggcag
     3181 cattgtttgc cggtattgcc gccatttttc tgaatcccgt ttctattgcg gtttctgcat
     3241 tggtaacgag tatcctgata atgggatcat ggtggcttta tttccgtgcc aactggcacg
     3301 ggcagggaca gaccttggcg atgaggacgt ggaaaatcgg tttgtgcgac cttaacggca
     3361 tacagccgtc tctacactta ctccgcctgc gctttatttg ggcgtgcata ttcatcgtct
     3421 tcattcctat gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg
     3481 cggccggcgc ggcattgatt tggctgattt taccgtgggg gttcgccctg ctgaatcccg
     3541 atcggcagtt tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc
     3601 cttaagcctt tataccgcaa aggtttcaaa ctgaaaaaat gccgtctgaa agggctttca
     3661 gacggcattt gcttatcggg gaaaccgatt attcgatatt ctgcacctgt tcgcgcatct
     3721 gttcgatcag gactttcagc tcgaccgagg cttgggtgca ttcggtagcg atggatttgc
     3781 tgcccaaagt gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgtttgccgc
     3841 tgctgccttt gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgtaggcggc
     3901 tgaactcttc gtcgatgtcg gatttttgga taaagagggc aaattcctgt tgcaggcggt
     3961 cgttgtcgat gctgccgacc gcttcggcaa ggcgggcgcg gattttttct ttatgtgttt
     4021 ccaacagggt aggaaagagt tcgcttaatg catctatgat ttcttccatg ctttcgaggc
     4081 gttgtaacag gtgctcgccc aattttttcc cttcgcgccg gcgtgcggcg gtaaagtcct
     4141 tcagtgccga atcggtcaaa tcggcaataa cctttgccaa tgcgtccgca ttttcccttt
     4201 gacttgccaa tacgcccgga aagcgcaaaa tatcggcaac gcccagtttt gccaaatcat
     4261 aatgcttgcg gaggtctttg ttgatttcgg caagctgtcc gaccagatcg cgattcagtt
     4321 ccaaagactg attgccgttt tccgcatctt gaatttggat tttgcattcg actttgccgc
     4381 gtgcgatatg ggatgaaatt ttctcgcgga taccgctttc caaatagcgc aaatcgtcgg
     4441 gcatcctgat ttgaatatct agaaaacggt ggttgaccgc acgcaggtcg agattgatgc
     4501 gtttgctgcc gcactctgcc gctgcgttgg caaatccggt catgctgtgg atgtggatat
     4561 ttccgctgct catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt
     4621 atggcggcaa gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc
     4681 acgcgctttt gttgctgcaa ggaagggaaa tgtatagtgg attaaccaag accagtacgg
     4741 cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca ccaagtgaat
     4801 cggttccgta cgatttgtac tgtctgcggc ttcgccgcct tgtcctgatt tttgttaatc
     4861 cactatatca attccgccaa tctgtcggaa aagcagctga tgcggcagtg tctggtgcat
     4921 gtctgctttt tgatttcggc aattgcaacg gcgtggacgg ataaaatcgt gtacagcacg
     4981 acgcacaaac cgcattgatg tttaccaaat aaaatacccg acaaaacaat ttgtcgggta
     5041 ttttattgcg tatatttcaa accgcttcgg cttcttcggt caggaaacca cgcagtttct
     5101 gcatggcttt tgcttcaatc tggcggatgc gctcggcaga gacgccgtat tctgcagcaa
     5161 gctggtgcag cgtcagcccg ccgtcgtctt gaagccagcg gctttccaca atacggcggc
     5221 tcctgtcatc cagttgcgcc aaagcgtttt gtaaaccttc tgtttgcagg gcgtaatgcg
     5281 cctgtttcga tagttgccgg ctcggttcgg aatcgtggtc ggcaagccag tcgatggggg
     5341 cgaaactgtc ctcgtcgtcg ctgttgtctg ccatgatggc gatgtcgtgt cccgtcatgc
     5401 gctgttccat ttccagaact tcggaaagtt tgacacccaa atcgtcggcg atgtcttgtg
     5461 cctctttggg agacagggcg ttgaggtttt tacgcatact gcgtaaattg aaaaacagct
     5521 tgcgttgcgg tttggtggtg gcaacgcgaa ccaaacgcca gtttctcaaa ataaactcgt
     5581 ggatttcggc tttaatccag tgtacggcaa atgaaaacag acgcgcgcct ctaccgggct
     5641 tgtagcgttt gaccgccttc atcagtccga tattgccttc ctgaatcagg tctgcctgat
     5701 tcagcccgta gccgtcatag ccgcgcgcga tggaaacgac gacgcgcagg tgggacagga
     5761 tgagttgttt ggcggcgttg aggtcgccct ttatccggcg ttcggcgagg cgggtttctt
     5821 cttcctggga cagcatcggg atgctgttga cggtgtggat gtattgttcg aggctgccgt
     5881 tgccgctttg gatggcgggt aatgcgaaag cgttattcat ttggggcatt tcctttcggc
     5941 tgaaactgcg tatcggcggt ttgctgtgtt gggatgcagt atatcactgc ttggcttgta
     6001 ttttgtatgt ttggcaggag atatgcgcta aggtttgaaa gacaggaaaa ttttgtaagg
     6061 caggtttgat tgattttgta aacctgatga ctcaattcga tttcagaatt atattacata
     6121 cgtagttgta tgtaaacggc cggtttgaaa aaagacagcc cgtccggacg ggctgtgcag
     6181 gtatcagtgt tctttgtttc ggaagatgaa aagaatcagt gcggctaggg tcaatatgcc
     6241 catcaaccac catgaactgc cggttttcat atagggcgtt tcgccgacat agcctttgat
     6301 gtgtccttcc aatacggttt ccgtatcggg ttgggcttgg gcgatgatgt tgcctttggg
     6361 ggagatgatg gcggttgcgc cggtgttggt ggcacggacc atatagcgtc cgagttccat
     6421 agcccgtgcc tgcgattgtt ggaggtgctg gtacatggcg ttggattttc cgtaccacgc
     6481 catattgctg gcattggcaa gcagggtggc atctttggcg gcggcaatca gttcgtcgcc
     6541 gaatccgtct tcgtaacaga tgttgaaggc gattttttgg tttttcatca gcagggcgga
     6601 ttgcttaccg ccgcctttgc ggaagtcgga aaggggcata ttcatcattt tgtaaagcgg
     6661 cgtggtcagg aagggcagcg gtttgtattc gccgaagggg acgaggtggt ttttggcgta
     6721 gtaggggatg ccgtcctgat tgttttcctg ataaccggtc aggttgatga cggcgttttc
     6781 gtaaccgttg ccgtccgaag tgtattggct gatgccgacg gcgagcgcgc tgccgttgtt
     6841 ttgcgcctgt tcggcaaatt ttgccagtat gttttccggc aggttttggc gcataacggg
     6901 gatggcggtt tcgggcagga tgacgatgtc ggcagtggtt ttgccgacct gttcgtaata
     6961 tttctgtatg gtcgggatga cttggtcttc acgccatttg agggtttggt cgatgttgcc
     7021 ttgaagcagg gcgacggtgc tgcggctgcc gtcggggcgg gtgaagtcgg tttgtcgggc
     7081 ggtgtagcct gcggcaagca gggcggcaat caggataatc ggaagcaggc gtttgcccga
     7141 acgtgcggtg ttatcactcg ccaaaaccag ccagacaccg agaaaggcgg ttgccagtgt
     7201 aaccatgtgg atgccgccca atggggcaaa gccggcgagc gggctgtccg gggtgatttg
     7261 ggagtagccg attgcgcccc agccgaatcc ggtcaggaaa cgctcgcggg caaactcggt
     7321 cagcgtccac aggatgggca gtaccaaacc gatttttatg ccccgaggca gggtaaattt
     7381 tttccacagc cagaaacaca gtgccggata aagggcaagg taggcgggga gtaggaaggt
     7441 cagcggtacg gcatagaggt cgggcaggcc ggaaacgtcg tgcagggcgg tgtgtatcca
     7501 gtagaactgt gtcgtgtatg cggtcaggcc gaacaggtag gcggaagaga cagcaaaacg
     7561 cggacgcagt tcgatgaggc ggacgaaggc accgaaaatc aagggcatca gccaaaagtg
     7621 gtagtagggt gcgaaggtaa agggggtggc ggcggcaaaa aggatgagca aaggccagta
     7681 gagggcgggg tgctgccagt atttgtccag tttggaaacc atattcatct gtctgttcgg
     7741 aagataccgt ctgaacatct ttcaaacggc atcggtattt gaaaaagaaa tcaatgcctg
     7801 ccgaaacgat tcatcagcgg caaggcgggg gcgcaggcaa tcgaaccgga aagcagcccg
     7861 acaacgaggt tggcgaagta ctccgcccag ccagcgtccc agtgttgcgc gtgcaggaaa
     7921 tcgtgcagaa ggccgaggtt gtgggcgacc agtacgccgc cgataagaaa catggcaagc
     7981 gtgccgacca cgctcaaacc gcgcataaag caaggcataa aggcagtcag catttgcccc
     8041 aaactgcgcg aaaaggtttg tgggcggcgc atcagcagca tgcctaagtc gtcgagtttg
     8101 acgatgacgg caacgattcc gtacaccaaa acagtcatgc cgatgccgat tgccgccatt
     8161 acgagcatgc gcgtcatgtc ggtgcagaaa cttgtgcagc agcttttcta cgccttcaaa
     8221 gcacagataa atgccgcctg ccgtcaaaag cggcgtaatg agttgcggca ggaaggcgga
     8281 aagcagcagg gccgcaggca ccaaaaccgg cttgttggaa aaagaacctt tcgccatcga
     8341 ccaaacaatc ggcaactcgc gttctgccga tacgcccgta acccggttgg cattgggtgc
     8401 caaatcgtcg ccgaccatgc cggcggtttt ctttgcggcg gctttggtca tcagggcaac
     8461 atcgtccaaa acggcggtga tgtcgtccgg cagggtaaat agtgaggcaa atgccattaa
     8521 agaatcctga aatgcggcgc aaagtccgac attatatagg agaacgcgga tttgggcggt
     8581 ttcaggcggc atgaaacagg aaaatgccgt ctgaaggcgg tggcggacgt gaagtaaagt
     8641 ttcgtgaaaa aaatgccgtg ttacagtctt tcgattttaa tttaatgaat tttaagggag
     8701 aatcgttagc gtggattgga tgggcagtct gttcctgccg ggtggcgcac tgttgttcct
     8761 gagcgtggtt tcgaccactt tgtccgcatg tttgggaatg cctttgctgc tggtttctcc
     8821 cgccggcgtg ttggacgggg cggcggaagc cttggcgatt gcggcgttcc tgatgctggt
     8881 cgcgcgtccg ccggcagtgt tcggcggttt gtggaaattc aattaca
//
LOCUS       DAXHPG010000076         8881 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXHPG010000076 DAXHPG010000000
VERSION     DAXHPG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.076"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            143..985
                     /locus_tag="ACFM6F_001293"
     CDS             143..985
                     /locus_tag="ACFM6F_001293"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC6164699.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSVEEGLETARAA
                     GVAV"
     gene            1015..1479
                     /locus_tag="ACFM6F_001294"
     CDS             1015..1479
                     /locus_tag="ACFM6F_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC6164700.1"
                     /translation="MKCPFCAHPDTRVADSRLMDERNAVRRRRYCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTDRKA"
     gene            1511..2620
                     /gene="ribD"
                     /locus_tag="ACFM6F_001295"
     CDS             1511..2620
                     /gene="ribD"
                     /locus_tag="ACFM6F_001295"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC6164701.1"
                     /translation="MFSETDISMMQNALKLAALGRFSTSPNPRVGCVIAQGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETADGK
                     IDLHRLMRLLADEGYGEVMVEAGAKLTSAFLAEDLADEIVLYRSPKILGSGKDLFSLP
                     ENRAALSAPPLWTPVSTEILGHDVKTVFRKNGNAF"
     gene            2723..4141
                     /locus_tag="ACFM6F_001296"
     CDS             2723..4141
                     /locus_tag="ACFM6F_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC6164702.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDILFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLLTV
                     GLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIPI
                     ALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFRA
                     IEANAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPPL
                     FCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFWL
                     FFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAVYLAGC
                     ILRHRKDLHKLFHYLKKQGFPL"
     gene            4138..5210
                     /locus_tag="ACFM6F_001297"
                     /pseudo
     CDS             4138..5210
                     /locus_tag="ACFM6F_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            5230..6396
                     /locus_tag="ACFM6F_001298"
     CDS             5230..6396
                     /locus_tag="ACFM6F_001298"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6164703.1"
                     /translation="MNITIVAPYCSLPSEPYFNRFWYLAERLSQSHDVLLITSRFRHY
                     DKSFRRPEDAEAASQDRLKVMLLEESGYGKNVSLERVASHYRFVKNFEQWLKNCRPGE
                     QDIVYSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRCADALVAVSQTYLDRAKEANPNVPGEAVYIGTDFAAIAPPPRFRSKTVRL
                     FYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLEKLKQYENRAIKFYGYLP
                     YSEMMSIAKGCDIAVNAIHSHAMQSVTNKLSDYMALQKPILNSQNNAEVLDLMNLLPH
                     ENYRSGDVDGFVQAAKDILKRKSDPVQSDEIVRRFKRDVAYQKIVNLIERLAHE"
     gene            6389..6916
                     /locus_tag="ACFM6F_001299"
                     /pseudo
     CDS             6389..6916
                     /locus_tag="ACFM6F_001299"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            6965..7966
                     /locus_tag="ACFM6F_001300"
     CDS             6965..7966
                     /locus_tag="ACFM6F_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFC6164704.1"
                     /translation="MPYFTGNQTNEKNNILILSAGRRVELVQDFQTEAARFSDGISVF
                     ATDLNPRMSSACHVSDGSFEVPPISADSYIDSIFNLAVRENIGLIIPTIDTELQKLAD
                     ERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRSPEIYDRKKLVFPCFAKPY
                     DGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFSEFTVDMYYDRQCRLKCAI
                     PRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCITAQFFYNEETGEFYGVEI
                     NPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWENNLIMLRYDAKVLVHEND"
     gene            7953..8639
                     /locus_tag="ACFM6F_001301"
     CDS             7953..8639
                     /locus_tag="ACFM6F_001301"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6164705.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
BASE COUNT         1990 a         2785 c         2193 g         1913 t
ORIGIN      
        1 gcgggaatga cagaacctta agtttccgtc tttgttttct gttttcgcgg gaataatggg
       61 gaacaaaccg tatttcagac ggcattttca gttcggggta taatccgaat acttgcgacc
      121 atctgaatca ttgggacaaa ctatgtgtca actgctgggc atgaactgca atacgccgac
      181 cgatattatg ttttcctttg aaggcttccg ccggcgcggc ggcattaccg accatcatgc
      241 cgacggtttc ggcatcggct ttttcgaagg caaaggcgtg cgcctgttcc atgacgacaa
      301 acccagtgcc aattcgcccg tcgccgacct cgtgcgcgcc taccagatta aatcggaaaa
      361 cgtcgtcgcc catatccgca aagcatcgca aggacaaacc tcgctggcga acacccatcc
      421 ctttatgcgc gaaatgtggg gtggctactg gctgtttgcc cacaacgggc atttgattga
      481 ttttttcccc gaacagggcg aatttttcca ccccgtcggc acaaccgatt ccgaacgcgc
      541 gttctgccac atcctcaacc gcctgcgcac ccgctttgcc gcccgtcccg acgacgacac
      601 gctgtttgac gcgattgcgg ggctgacgca cgaaatccgc aagttcgggc tgtttaactt
      661 tatgctttca gacggcattg ccctgtttgc ccacgccagc acactgctgc actacatcgt
      721 ccgccaagcc ccgttcggca aggcgcgcct gctcgacgac gacgtgatgg tcgattttgc
      781 cgaagtaacc acgccctccg accgcgtcgc cgttatcgcc accctgccgc tgacccgcga
      841 cgaatcatgg tcccaacttg ccgtggacga actggtcatg ttccgcgaag gcaacatcgt
      901 ccgacacgac cgtcccgaaa accccgttta tatgagtgtc gaagaaggtt tggaaaccgc
      961 gcgcgccgcc ggcgtcgccg tctgaacttc agacgacata ggaggacgaa cccgatgaaa
     1021 tgcccgtttt gcgcccaccc cgacacccgc gttgccgatt cgcgcctgat ggacgaacgc
     1081 aacgccgtcc gccgccgccg atactgcccc aactgcggca aacgcttcgg cacgctcgaa
     1141 accgccgaac tcaaaatgcc cgccgtcatc ggtccggaca aaaaacgttc gccctttaat
     1201 gcacaacgcc tccgcaacga cctgaccgcc gccgcccgaa aatccgccct gacacccgaa
     1261 cagatcgacg aaaccgtccg cctgacggaa cacaggctct acacttcggg tcagcgcgac
     1321 atcccctctg ccgcacttgc cgacatggtg ctaaaagaac tgctccgaca agatacggaa
     1381 gccgccgtcc gtttcgccgc cctgcacaaa cgcttcgaca atccggcaga ctttgcctcg
     1441 tggctggcgc aagccgtcaa aacagaccgc aaagcctgat tcccccaacc catactgata
     1501 cggtatcccc atgttttccg aaacagatat ttctatgatg caaaacgccc tcaaacttgc
     1561 cgctttgggg cgtttttcca cttcgcccaa tccgcgcgtc ggctgcgtta tcgcacaagg
     1621 cagccaaatt gtcggacagg gcttccacgt caaagcgggc gaaccgcacg ccgaagtcca
     1681 cgccctgcgt caggcgggcg aaatggcaca aggcgcgacc gcctttgtta ccctcgaacc
     1741 gtgcagccat tacggacgca caccgccctg cgccgaagct ctgctccgtt ccggcgtaac
     1801 acgcgtcgtt gccgccatgc gcgaccccaa cccgctggtt gcaggcaaag ggcttgccct
     1861 gctcgaagca gcaggcatca agacggaatg cggtttactc gaacatcagg caagggaact
     1921 caaccgaggc ttcctgtcgc gcatcgaacg ccgccgcccc ttcgtccgcc tcaaatgcgc
     1981 cgtttcgctg gacggcaaaa ccgccctttc agacggcagc agcttttgga ttaccggcga
     2041 agacgcgcgt gccgacgtac aggttttgcg tgccgaaagc tgcgcggtgc tgaccggcat
     2101 cggcacggtg ttggcggaca atccccggct caacgtccgc gcttttccaa ctttgcgcca
     2161 acccgcacgc atcgtgttag acagccgcct gcgcctgccc ttggacagcc accttctgac
     2221 cgacggacaa tctccaacct acatcgccac cttggaacgc gatgaagaca aactccgccc
     2281 ctatcgggaa cacgcacata tccgcatcct gatgccgtct gaaacggcag acggcaaaat
     2341 cgacctgcac cgcctgatgc gcctccttgc cgacgaagga tacggcgaag taatggtgga
     2401 agccggcgcg aaactcacat ccgccttttt ggcggaagat ttggcggacg aaatcgtgct
     2461 gtaccgttcg cccaaaatcc tcggcagcgg caaagacctg ttttccctgc ccgaaaaccg
     2521 cgccgccctt tccgcaccgc ccttatggac acccgtttcc accgaaatcc tcggacacga
     2581 cgtcaaaacc gtattccgga aaaacggcaa cgccttttaa agggtttgcc gccgtttcac
     2641 tatataataa cgccgtcaaa ttccgatcaa aaaaggcttc agacggcacc ggcggcatcc
     2701 cgcctccgcc cggacatcaa aaatggacac aaaagaaatc ctcggctacg cggcaggctc
     2761 gatcggcagc gcggttttag ccgtcatcat cctgccgctg ctgtcgtggt atttccctgc
     2821 cgacgacatc gggcgcatcg tgctgatgca gacggcggcg ggactgactg tgtcggtatt
     2881 gtgcctcggg ctggatcagg catacgtccg cgaatactat gccgccgccg acaaagacat
     2941 tttgttcaaa accctgttcc tgccgccgct gctgtctgcc gccgcgatag ccgccctgct
     3001 gctttcccgc ccgtccctgc cgtctgaaat cctgttttcg ctcgacgatg ccgccgccgg
     3061 catcgggctg gtgctgtttg aactgagctt cctgcccatc cgctttctct tactggtttt
     3121 gcgtatggaa ggacgcgccc ttgccttttc gtccgcgcaa ctcgtgccca agctcgccat
     3181 cctgctgctg ctgctgacgg tcgggctgct gcactttccg gcgaacaccg ccgtcctgac
     3241 cgccgtttac gcgctggcaa accttgccgc cgccgccttt ttgctgtttc aaaaccgatg
     3301 ccgtctgaag gccgtccggc gcgcaccgtt ttcatccgcc gtcctgcatc gcggcctgcg
     3361 ctacggcata ccgatcgcac taagcagcat cgcctattgg gggctggcat ccgccgaccg
     3421 tttgttcctg aaaaaatatg ccggcctaga acagctcggc gtttattcga tgggtatttc
     3481 gttcggcgga gcggcattat tgttccaaag catcttttca acggtctgga caccgtatat
     3541 tttccgcgca atcgaagcaa acgccccgcc cgcccgcctc tcggcaacgg cagaatccgc
     3601 cgccgccctg cttgcctccg ccctctgcct gaccggcatt ttctcgcccc tcgcctccct
     3661 cctgctgccg gaaaactacg ccgccgtccg gtttatcgtc gtatcgtgta tgctgccgcc
     3721 gctgttttgc acgctggtag aaatcagcgg catcggtttg aacgtcgtcc gaaaaacacg
     3781 cccgatcgcg ctcgccacct tgggcgcgct ggcggcaaac ctgctgctgc tggggcttgc
     3841 cgtaccgtcc ggcggcgcgc gcggcgcggc ggttgcctgt gccgcctcat tttggctgtt
     3901 ttttgttttc aagaccgaaa gctcctgccg cctgtggcag ccgctcaaac gcctgccgct
     3961 ttatatgcac acattgttct gcctggcctc ctcggcggcc tacacctgct tcggcacgcc
     4021 ggcaaactac cccctgtttg ccggcgtatg ggcggtatat ctggcaggct gcatcctgcg
     4081 ccaccggaaa gatttgcaca aactgtttca ttatttgaaa aaacaaggat ttccattatg
     4141 aaaatcatcc tgaccacttc tatgtcgggc ttgggcggca cggaaaccgc caccgtccgc
     4201 ctcgggcggc tgctcaaacg gcgcgggcac gacatcatcc ttgcttcttc ggacgggcct
     4261 tttgtcggag aggcgcaagc atcgggcatc cgttggcagc cggttgattt ttactggggc
     4321 gggcttgccg gctacctcaa aagcaccttt gcctacgccc ggatgctgcg ccgcgaacaa
     4381 cccgacatca tcgactgcca aatggcgcgt gttgttccag cctgcgccct tgccgccaaa
     4441 atcgtttcgc cgaaaaccaa aatcatctac cactcgcacg gtttggacgc ggcaacctac
     4501 cccaaaatcg ccaaactctt cgacaagctg ggcgtgtaca tcatcggcaa ctgcaaacac
     4561 gagcgcgaaa aactcatccg ccacggcttc cccgcaggcc ggattgccta cgcctacaac
     4621 gcactccccc ccccccggaa tttcatttcc ggaaaaccga aaaagaatgc gccgtactcg
     4681 gcacgctttc ccgtttggac accgtccgcg ccgtgcatct gatgttggat attttgaaga
     4741 aaatggttga ctgcggtatg cccgtccgcc tcaacatagc gggcatagga gaagaaatgg
     4801 acaatttgaa agcgcaggcg gaacgtttgg gcatagatga caaagttacc ttcctcggcg
     4861 gcatacgcga cttgaccggc tacttcaaag aaatcgatat tttggtgaac acgccgcatt
     4921 gcgtgggcga ccacggcgca ggtgtcggca acaacatctt ggaagccggc ctttacgaca
     4981 cgcccgtcgt tacctacgat atggcgggca tttccgaaat ggtcattacc ggccgaaccg
     5041 gctactgcat ccccttcggc gacgaagaag cctttatcgc cgccgtagac acgctcgtca
     5101 gacaaccgga gttgcgcgaa gcaatgggca aaaggctgca cgcacacgtc gccgcactct
     5161 gttccgacga cgaaatctac cgtaccacca tggcggcgta tgccatgtaa tgccgtctga
     5221 aagccgccga tgaacatcac catagtcgcc ccctactgct cgctgccgtc cgaaccgtat
     5281 ttcaaccgct tttggtatct ggcagaacga ttgtcgcaat cgcacgacgt gttgctgatt
     5341 accagccgtt tccgccacta cgacaaatcc ttcagacggc ccgaagatgc cgaggccgcc
     5401 tcgcaagacc gtctgaaagt catgctgttg gaagaaagcg gctacggcaa aaacgtgtct
     5461 ttagaacgtg ttgccagcca ttaccgcttc gtcaaaaatt ttgagcaatg gctgaaaaac
     5521 tgccggccgg gcgaacaaga catcgtctat tccgcctatc cgctgattgc caccaacctg
     5581 ctgttgggca aacacaaagc gcgtttgggc tacaaactga ttgtcgatgt gcaggacgta
     5641 tggccggagt ctttctcctc ggtcgtaccg tttttgaaaa aagtaccgca caagctgctg
     5701 ccctttgctt cacgcgccaa ccgtgcctac cgctgcgccg acgcgctggt tgccgtatcg
     5761 cagacttatc tcgaccgcgc caaagaagcc aatccgaacg tacccggcga agccgtctat
     5821 atcggtacgg attttgccgc catcgccccc cccccgcgat tccgctccaa aaccgtccgc
     5881 cttttctact tgggcacgct cagttacagc tacgacgtgg aaaccgtgtg caaaggtgtt
     5941 cggaaacttt tagacgacgg cgaaaacgtc gaattgcaca ttatgggcgg aggacccgat
     6001 ttggagaaac tcaaacaata tgaaaatcgc gccatcaagt tttacggtta tttaccctat
     6061 tctgaaatga tgtcgattgc caaaggctgc gacattgccg tcaacgccat ccatagccac
     6121 gccatgcagt cggttaccaa caaactctcc gactatatgg ctttgcaaaa accgattctg
     6181 aacagtcaga acaatgccga agtcctcgac ctcatgaatc tgctgccgca cgaaaactac
     6241 cgttccggcg atgtggacgg tttcgttcaa gccgccaaag atattttgaa gcgcaaaagc
     6301 gatcctgttc agtccgacga aatcgtccgc cgcttcaaac gcgatgtggc gtatcagaaa
     6361 atcgtcaacc tgattgaaag attagctcat gaataaattt ttcaaacgcc tgttcgacat
     6421 catcgcctcc gcatcggggc tgattgtcct ctcacccgtt ttcctgattt tggcgtacct
     6481 catccgcaag aatctaggtt cgcccgtctt cttcattcaa gaacgccccg gaaaggacgg
     6541 aaaacctttt aaaatggtca aattccgttc catgcgcgac gcgcttgatt cagacggcat
     6601 tccgctgccc gacagcgagc ggctcacacc cttcggcaaa aaactgcgtg ccgctagttt
     6661 ggacgaactg cctgaattat ggaatgtctt aaaaggcgac atgagcctgg tcggcccgcg
     6721 cccgcttttg acgggctacc tgccgcttta taacgacttc caaaaccgcc gccacgaaat
     6781 gaaaccgggc attaccggct gggcgcaggt caacgggcgc aacgcgcttt cgtgggacga
     6841 aaagttcgcc tgcgatgttt ggtacaccga caacttcagc ttttggctgg atatgaaaat
     6901 cctgtttttg acagtcaaaa aagtcttgat caaagaaggc atttcggcgg aaggggaagc
     6961 cactatgcct tatttcactg gaaatcaaac taatgaaaaa aataatatac tgattctgtc
     7021 ggcaggtcgc cgtgtcgaat tggtgcagga cttccaaacc gaagccgcac gtttttcaga
     7081 cggcatcagc gtttttgcca ctgacctcaa cccccgcatg tcttctgcct gccatgtttc
     7141 ggacggttca tttgaagtgc cgccgattag tgccgattca tatatcgaca gcattttcaa
     7201 tctggcggta cgcgaaaata tcggtttaat catcccaacc atagacaccg aactgcaaaa
     7261 gctggcagac gagcgcgaac gctttgaagc ggcaagtgtc cacatcgtcg tttccgattc
     7321 ggacttcatc gcacaatgcc gcgacaaacg gaaaaccgcc accttgtttg cccgctacgg
     7381 catacgctca cccgaaattt acgaccgcaa gaagttggtt ttcccttgct tcgccaagcc
     7441 ttatgacggc agccgcgcca tcggtgcaaa acggattgac acacctgccg acctgacacc
     7501 ggaagtcttg gcagacacca aattaatgtt ctgtcagttt atcgacatcg aaaataaatt
     7561 ttccgagttt accgtcgata tgtattacga ccgtcaatgc cgtctgaaat gcgccatccc
     7621 gcgaaaacgc ttggaagtgc gctccggcga aatcagcaaa ggcgttacct gcaaaaacag
     7681 cctctaccaa acgctgctgg aaaaaatgtc ggtattgcag ggcgcgcgcg gctgtatcac
     7741 ggcgcagttc ttctacaacg aagaaaccgg cgaattctac ggcgtggaaa tcaacccccg
     7801 cttcggcggt ggcttcccgc tgacgtatgc cgcaggcggc aattatccga gccggttgat
     7861 gcgcgaatac ctcggcgacg aagaaatccc gttttcagac gactgggaaa acaacctcat
     7921 tatgttgcgc tacgatgcca aggtcttggt tcatgaaaac gattaatcct gaaaccaccg
     7981 tcatcgtctt cgacttggac gataccctgt attcggaata cgaatacaag ctctccggta
     8041 tccgttcggt catcgatacc gttgccgcac tgtatcccga ttggaattca gacaacctat
     8101 ggcgcaacat cgaccccgac ggcaaagatt ggctggacaa attgtgccgc cactgtggtt
     8161 ttaatgaatc ggaaaaacag gttcttttgt ggcaataccg gctgcaccgc ccgacgctga
     8221 ctccgtatgc tccgccggat ttcctgtccg aactgaccgc gcccttcgcc gcacgcgcgc
     8281 tgattaccga cggcagaagc ctgacccaac gcctgaagct ggaagccttg ggtctgtctt
     8341 ccctgtttga cgacatcctc atctctgaag cctgctcttc ggaaaagccc gacggcaaac
     8401 gcttccgcca tcttcaagac aaatatgccg acaaggcagg ctgcttcatc tatatcggcg
     8461 acaacatttc caaagacttt atcgcgccga acactttggg ctggataacc ataggattgc
     8521 ttccctccgg acagaacatc caccgccacc agcctgaaag ttttaacaca gaatatcatc
     8581 ccgacttttg ggttaactcc cttcaagatt tagttcctat aattgattca aatacttaac
     8641 cattacataa tcaaacatgc actcacaata ttaaaaggac tatttcatga caaaatatta
     8701 ttcatggggc gtaaacggct ttccgcccta cgttttcgac accatttgga aacgatggaa
     8761 gttgaatggg cgcgggggcg gttatcgtgt gcgacatccc ggacggcatg accgtcgcgg
     8821 gcaatccggc aaagccgctg ccgcgcaaaa accccaagac cgggacggca taagcgatta
     8881 a
//
LOCUS       DAXHPG010000077         8795 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXHPG010000077 DAXHPG010000000
VERSION     DAXHPG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8795
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.077"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(175..867)
                     /locus_tag="ACFM6F_001302"
     CDS             complement(175..867)
                     /locus_tag="ACFM6F_001302"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6164706.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGAPKYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(975..2168)
                     /locus_tag="ACFM6F_001303"
     CDS             complement(975..2168)
                     /locus_tag="ACFM6F_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6164707.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="ACFM6F_001304"
     CDS             complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="ACFM6F_001304"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFC6164708.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3021..3950)
                     /locus_tag="ACFM6F_001305"
     CDS             complement(3021..3950)
                     /locus_tag="ACFM6F_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFC6164709.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQVYLNQIRRITHEAG"
     gene            complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="ACFM6F_001306"
     CDS             complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="ACFM6F_001306"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFC6164710.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4778..6985
                     /gene="uvrD"
                     /locus_tag="ACFM6F_001307"
     CDS             4778..6985
                     /gene="uvrD"
                     /locus_tag="ACFM6F_001307"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC6164711.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVNVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASQEQGVSLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMPLPEIIAGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPDNISDDPAFPILAFLSNAALESGENQAGVGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7342..7881
                     /locus_tag="ACFM6F_001308"
     CDS             7342..7881
                     /locus_tag="ACFM6F_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6164712.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8071..8286)
                     /locus_tag="ACFM6F_001309"
     CDS             complement(8071..8286)
                     /locus_tag="ACFM6F_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164713.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8410..8769)
                     /locus_tag="ACFM6F_001310"
     CDS             complement(8410..8769)
                     /locus_tag="ACFM6F_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6164714.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
BASE COUNT         2189 a         2439 c         2189 g         1978 t
ORIGIN      
        1 cctctttcag ttacagcagc ttgatccctt tcccttatcc aacgggggaa ggctaggata
       61 gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca accctctccc
      121 tccgtacggg agggggtgga ttctcgcggg cgaagcccac gctacggtta gcctttaccc
      181 cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt cggtatgcgg
      241 cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc ggaggtggca
      301 gagttcgtgg atgcagacat agtcggcaac gtacttgggc gcgccgatca gccgccagtt
      361 gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt tgctcaggtc
      421 tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg gcagcaggta
      481 ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg cggtttcttt
      541 tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt gggtatccag
      601 cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg aaacaggatg
      661 cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca gccagtttgc
      721 taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca tattgacggg
      781 gcgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc acaagccgtc
      841 ggaaagagta taggtgaagc gtttcatagt tgtgaatagg tttcagaccg gatacatcgt
      901 ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa aatcaaacat
      961 ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt gatggcgctg
     1021 agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag cgggtgtttg
     1081 tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag ggaaatcggt
     1141 aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt gccgatggtg
     1201 gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt attgcgttcg
     1261 atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc aatcaccgaa
     1321 gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta tcccgccgca
     1381 tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg gctgaccacg
     1441 cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcaggat cgcccgcacc
     1501 gagctttggc tcacttccgg caacgccgat ttgcctttga tgcctgcgtc cagcagacgg
     1561 tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt aacaatcgcc
     1621 attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag cggcggattc
     1681 gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac ttgggcgaat
     1741 ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat cacgccgctg
     1801 atcacggcac cggtttccgg cgacagtccg gtcagcaggt tcagacccaa gcctgcgccg
     1861 ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc caagaaatag
     1921 cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga aacggcgaca
     1981 atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag aatcacaaag
     2041 ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa gcccgggccg
     2101 atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttctgcg gtttttttga
     2161 ttagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg cgaaacctgc
     2221 ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg ttttcagacg
     2281 gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt tcctgccgga
     2341 ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg ctgtcggctt
     2401 gcacggcaac caggctgccg ttcactgctt tgacccgccc gtcccgcacc atctgcaata
     2461 cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg cggggaacca
     2521 gcgtaccgtc gggcatatag cggcggtcgg cgaatacttc ggaaatcaca cccaagcctg
     2581 cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc aatttcgggt
     2641 cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc gccgcttggt
     2701 tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca cggcacaagg
     2761 cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg gacagattca
     2821 tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg atgcgcacgc
     2881 cgttttgttg ggcatacgcc aatgccgccc gaatatcggc aatgctgccg gcgtgttggg
     2941 cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg ccgcagcctt
     3001 cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat ttggttcaga
     3061 tagacttggt ttttgcgccg cagggcggtg gcttctttca agccgattat tttgaatttg
     3121 actttgctgc cgaagcgcac ctgtgccagc ctgcccaaat cggcggcggc aacggtagcg
     3181 attttcggat aaccgccggt ggtttgcgca tcggccagca ggataatcgg tttgccgccg
     3241 ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa aggttgcgac
     3301 agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct ttgcagcgtc
     3361 cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata ttccgacgaa
     3421 ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt ggacaattcc
     3481 tgcgcacctt tgccgatggg gagacaatca cctttttgca gcattctgcc ctgatggccg
     3541 ccgaaaccgg ctttcaggtc ggtgcttctc gaacccatca cttccggcac atcaaatccg
     3601 cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccatttt caaggtctgc
     3661 cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa ttccgcctga
     3721 tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat tatcccgccc
     3781 aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt acccgccgcc
     3841 aaagcaaccg tgtccatcgc accggcatga ccgatgccgt aacgccggtg tccgtagcgt
     3901 ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa cgtgaatcat ggctcaatcc
     3961 tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga ttcaaatcgg
     4021 ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc gaagcgaacg
     4081 gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg gacgttctcg
     4141 gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa gggaaaccgg
     4201 gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg acgatttcgg
     4261 aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat tcgccgccgt
     4321 agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg tgttcccaca
     4381 catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggta aacacggtca
     4441 gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc gcagcggcaa
     4501 acgcccacaa cttttgctgt ttgcccagtt cggaaggcgc attcagtcgg cagaccaaag
     4561 cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg ttatgtttta
     4621 atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat catcaaaccg
     4681 tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa accgtttcta
     4741 ttaaaatagc gcattccact tttcagacgg catccttatg tttcccgacc aatccgcccc
     4801 caacctgctg caaggcttga atcccgaaca actctccgcc gtaacctggc cgccgcaatc
     4861 cgcacttgtg ctggcgggcg cgggcagcgg caaaacgcgc gtgctgacca cgcgcatcgc
     4921 atggcttttg caaagcggac aagccagcgt gcatagcatt atggcggtaa cgtttaccaa
     4981 caaagccgcc aaagaaatgc agacccgttt gggcgcaatg attcccgtca acgtccgcgc
     5041 catgtggctc ggcacgtttc acggtctctg ccaccgcttt ttgcgcctgc accaccgcga
     5101 cgcaggcctg ccgtcttcct ttcaaatcct cgacagcggc gaccagcttt ccctcatcaa
     5161 acgcctgctc aaaagcctca acatcgccga agaaatcatc gcgccgcgtt cgctgcaagg
     5221 ctttatcaac gcgcaaaaag aatccggttt gcgcgcttcc gtcttgagcg cgcccgaccc
     5281 gcacacaagc cgcatgatcg aatgctacgc cgaatacgac aaaatctgcc aacgcgaagg
     5341 cgtggtcgat ttcgccgagc ttatgctccg cagctacgaa atgctgcaaa gcaacgaaat
     5401 cctgcgccag cattaccaaa accgcttcaa ccacattctc gtcgacgagt tccaagacac
     5461 caacaaactg caatacgcct ggctcaaact catggcgggc ggaaacgcag cggtatttgc
     5521 cgtcggcgac gacgaccaaa gcatttaccg tttccgtggc gcaaacgtcg gcaacatgac
     5581 cgcgctgatg gaagaattcc acatcgacgc gcccgtcaaa ctcgaacaaa actaccgctc
     5641 cgtcggcaac atccttgccg ccgccaatgc cgttatcgaa aacaacgacg aacgactcgg
     5701 caaaaacctg cgcaccgacg ccgaagcagg cgacaaaatt cgctactact ccgcctttac
     5761 cgacctcgaa gaagcccggt tcatcgtgga cgaaaccaaa gccctcgaac gcgaaggctg
     5821 ggatttggac gaaatcgccg tcctctaccg tagcaacgcc caatcccgcg ttatcgaaca
     5881 aagcctgttc cgcagcggca tcccctacaa aatctacggc ggcttgcgct tttacgaacg
     5941 ccaagaaatc aaacacgccc tcgcctacct gcgcctcgcc gtcaatcccg acgacgacaa
     6001 cgccctcttg cgtgtcatca acttcccacc gcgcggcatc ggtgcacgta ccgtcgaaaa
     6061 tcttcagacg gcatcgcaag agcaaggcgt ttcactttgg caggcagcct gcaatgccgg
     6121 cgcaaaagcc gccaaagtcg ccgccttcgt ccgcctcatt gaagccctgc gcaaccaagt
     6181 cggacaaatg cccctgcccg aaatcatcgc cggcatcctc aaagacagtg gcttgaccga
     6241 acactaccgc acccaaaaag gcgacaacca agaccgcctc gacaaccttg acgaactcgt
     6301 caacgccgcc atcgagttca aacccgaaga cagcaacttt gaaaccctgc ccgacaacat
     6361 ttcagacgac cccgccttcc ccatcctcgc tttcttgagc aacgccgccc tcgaatccgg
     6421 cgaaaaccaa gcaggcgtgg gcgaaaaagc cgtccaactc atgaccgtcc acgccgccaa
     6481 aggcttggaa ttcaacgccg tcttcctcac cggtatggaa gaaggccgtt tccccagcga
     6541 aatgagcctt gccgaacgcg gcggcctcga agaagaacgc cgcctcatgt acgtcgccat
     6601 cacccgcgcc cgcaaacgcc tctacatcac catggcgcaa caacgcatgc tgcacggaca
     6661 aacccaattc ggcatcgtct cccgcttcgt cgaagagatc ccacccgaag tattgcacta
     6721 cctgtccgtc aaaaagaccg cctacgacag ctacggcagc ccgcgccaaa ccgccgcatc
     6781 caaagacaaa atcatcgacg actacaaaca gccccaaacc tacgcaggtt tccgtatcgg
     6841 acaaaacgtc cgccacgcca aattcggcac cggcgtgatt atcgatgccg cagataaagg
     6901 cgaatccgcc cgactgacca tcaacttcgg caaacagggc gtgaaagagt tggataccaa
     6961 gtttgcgaaa ttggaagcga tgtaaatttg aaatgcaggc tggatactcg tatccgacaa
     7021 aaatatttga cgcgtctatc gtttccgaaa aaccgctgtt ggaaaatgtc ggattcgaga
     7081 atccgaccta tggcaaaaaa ctgtagcaag gacaaggcaa aaggccgtct gaatccggta
     7141 acaacaaatt ttgccgaaac ctccgccgtc attcccgcgc aggcgggaat ccagaccttt
     7201 cggcacggga acttatcgga taaaaggttt ctttagattc cacgttctag attcccgcct
     7261 gcgcgggaat gacggacggt aaaagccggg tatagatacc taacccttgt ttatagctga
     7321 aaatcaataa ggaagaacat tatgtcccaa gtttttaaag attttgattt gtcctccgta
     7381 tgggaatccg acagttgggc agatgaaaac tacaaagaag ccccgtttac ccctgaaatt
     7441 ttggctgccg tagaaagtga actgggctat aaattgccgc aaagttttat tgaattgatg
     7501 gcggtacaaa acggcggaat atttgtcaaa aactgttttc cgaccacaca gagaaattcg
     7561 tgggcgaaag atcatgtgca aatttgcgag gtatcgggca tcggctttga aaaagaaggg
     7621 agtttgtgcg gcgaaacagg gcaaaaactt tggatggaag aatgggaata tccgcccatc
     7681 ggcgtgtatt ttgccaacga cccgtcaggc ggtcatgcca tgtttgcctt ggactatcgg
     7741 gagtgtggaa aagacggcga gccgaaagtg gtgtttgtcg agcaagaatc ggattttgaa
     7801 atcgtcgaac ttgcccccga ttttgaaacc tttatccgca gcttgcggca tgaagatgag
     7861 tttattgacg aggaaatata aaacggcggt tgaaaaaccg gaatcatcaa gggaaaacgg
     7921 gcgaaataac ggataatcgc ttgaatccgt aaggaaaacg gtttggtgga acgcgccatc
     7981 caagaccttt gcaaagaaaa actgtctccg acagcattga cattattaac agaacttatc
     8041 aagtttggag ctatctcaaa tataattcgg ttatcctttt gtatccatta aatcatatgc
     8101 ttcaattaat tgttgttcta gctcttgtac caattttgaa ttgcgaattc ctgacacaat
     8161 ctcaaattct tctgcatcta tgcaaacacc tgcataaatt tcaataacaa gggaacgcaa
     8221 taattgaagc tcttctcttg ttaaagaaat aataatgtca tcacctgtgt aattgattat
     8281 attcataata attttatttt tgtttgtcaa agtaagtttt gcctaaggtt ggtctaaatg
     8341 cagttccacc atcttttgaa tttgggtctc tgattacaat tgctccagac ttatcatccc
     8401 aaattgctct tatgtgtttg gattgtaatc ttcgaattcc caagaaaaaa atcgtaataa
     8461 gtttgaaagt gtcaaatccc aagtttcttt tgagcaatat tctaatattt tatcaatttc
     8521 acttttaata atcttatgat caaactgttc taatattaat gcattagacc aaaaaaaacc
     8581 ttctttatta caatgatggg aaatccattt aggagaacaa atgcaaagtg aaaaaataga
     8641 tgagccttgt tctccttcga ttccgatatc caaatctatc catctatgga aattatctgg
     8701 aatttcgggg gtaaattttt caaaatcaat atcatataaa tttatgcttt ttaaatccaa
     8761 tttaatcatt agggctgtcc tagataaata gggaa
//
LOCUS       DAXHPG010000078         8793 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXHPG010000078 DAXHPG010000000
VERSION     DAXHPG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.078"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            75..299
                     /locus_tag="ACFM6F_001311"
     CDS             75..299
                     /locus_tag="ACFM6F_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164715.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            355..1011
                     /locus_tag="ACFM6F_001312"
     CDS             355..1011
                     /locus_tag="ACFM6F_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6164716.1"
                     /translation="MEQKRRFAASLLLAAVLPLCAHSFPFAEENPIAYGKVKIQSWKE
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLKKLDKEQMRASFEDMQRIMP
                     DLGFEAKGYALSFEQLAQLKIPIIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRVQFLDAWKTREGNLAGKILAVVPKKAETISNKLFFTHHPKRQTEFAVKQIRLRRIE
                     "
     gene            1129..2424
                     /gene="hisS"
                     /locus_tag="ACFM6F_001313"
     CDS             1129..2424
                     /gene="hisS"
                     /locus_tag="ACFM6F_001313"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC6164717.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRAYG
                     YRQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIITMSADLW
                     EKLSIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVGEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTNTLQQWKNA"
     gene            2425..3054
                     /locus_tag="ACFM6F_001314"
     CDS             2425..3054
                     /locus_tag="ACFM6F_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC6164718.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAILAEIVEKAQSKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            3208..4665
                     /gene="der"
                     /locus_tag="ACFM6F_001315"
     CDS             3208..4665
                     /gene="der"
                     /locus_tag="ACFM6F_001315"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC6164719.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEEIL
                     ENFPEPEVEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENADDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            4696..5595
                     /locus_tag="ACFM6F_001316"
     CDS             4696..5595
                     /locus_tag="ACFM6F_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC6164720.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            5647..6063
                     /locus_tag="ACFM6F_001317"
     CDS             5647..6063
                     /locus_tag="ACFM6F_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFC6164721.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            6129..6695
                     /gene="dcd"
                     /locus_tag="ACFM6F_001318"
     CDS             6129..6695
                     /gene="dcd"
                     /locus_tag="ACFM6F_001318"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC6164722.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(6760..7464)
                     /locus_tag="ACFM6F_001319"
     CDS             complement(6760..7464)
                     /locus_tag="ACFM6F_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC6164723.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDELNRFIADIQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     QGVEEISISVNGTVQF"
     gene            7610..8793
                     /locus_tag="ACFM6F_001320"
                     /pseudo
     CDS             7610..8793
                     /locus_tag="ACFM6F_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         2341 a         2350 c         2117 g         1985 t
ORIGIN      
        1 tatctgatat atcaacatct ctctatctta attccgaatt ttattgttga aacttgcatg
       61 aaaaaccata caagatgatg aaaaacaaat atattttaat aaaagcagca atcggcataa
      121 ccgccatatc tatattttat cttctaactc aagaaagcag cggcaaaact gaagaaccat
      181 catatttctt gatgtttatg tttctcaact tgctttggtt tgaagaaaat aaaacagtta
      241 tggctgccgt tacggcaatg attgccgccc actttatatt tgtcgcacta tccgattaat
      301 aagcaatcaa aaaggctgct tggaaatttc aggcagcctt tcaggaggat tttgatggaa
      361 caaaaaaggc ggtttgccgc ttccctgctc ctcgccgccg tcctgccgct ttgtgcgcat
      421 tcttttccct ttgcggaaga aaaccccata gcctacggca aagtcaaaat acagagttgg
      481 aaagagaggc gcgatttcaa tattgtaaag caggatttgg atttttcctg cggggcggct
      541 tcggtggcga cgcttttgaa caatttttac gggcaaacgc tgacggaaga agaagtgttg
      601 aaaaagctgg ataaggagca gatgcgtgcg tcgtttgagg atatgcagcg cattatgcct
      661 gatttgggtt ttgaagcgaa aggctatgcc ctgtctttcg agcagcttgc acagttgaaa
      721 atccccatca tcgtgtatct gaaataccgc aaagatgatc atttctcggt gttgcgcggc
      781 atagacggca atacggtttt gcttgccgat ccgtcgctgg ggcatgtttc catgagcagg
      841 gtgcagtttt tggatgcttg gaaaacccgt gagggaaatt tggcaggtaa gattttggct
      901 gtcgtaccga aaaaagcgga gacgatttca aataaattgt tttttacaca tcatcccaag
      961 cgacagacgg aatttgcagt caaacaaatc aggctaaggc gtatagagtg agtcaaaggc
     1021 cgtctgaaaa ctgtttcaga cggccttttg atttgttccc aacctcacaa acccttaaaa
     1081 atcagctata atgcccaact atttgatttt cgcttatccc atattgaaat ggcacaaaaa
     1141 attcaatctg taaaaggtat gaacgacctt ttacctgtcg aacaaaaaga tttcaaactg
     1201 acggctgcgt tttggcaggc gtttgaagat acggtcggtc gttggacacg cgcttacggc
     1261 taccggcaaa tccgtacgcc gattgtcgag caaaccggtt tgtttgtccg ctccatcggc
     1321 gaggaaaccg atgtggtcgg caaggaaatg tacaccttct ctgattcaaa cgattcttta
     1381 agtttgagcc tgcgtcctga aggtacggct tcttgtctgc gtgcggtggt cgaacacaac
     1441 cttctgtaca acagcccgca aaagctgtgg tatatggggc cgatgttccg ccgcgagcgt
     1501 ccgcaaaaag gccgttatcg tcagttccat caggtcggta tcgaggcttt gggttttgaa
     1561 ggtcccgata ttgatgcgga aatcatcacg atgtctgccg atttgtggga aaaattgagt
     1621 attcgcgaat acctgacttt ggaaatcaac agcttaggca accgtgagga gcgagcggca
     1681 caccgtgcgg cgttggttga atatttgaca cgctatgaag ataaattgga cgaagacagc
     1741 aaacgccgtc tgaaaaccaa tcctttgcgc gttttagata cgaaaaaccc tgatttgcag
     1801 gaaatctgca atgccgcgcc gcgtttggtg gattacttgg gcgaggcttc gcaaaaccac
     1861 tatgcacgct tcaaggcgat gttggatggt ttgggtattc aatatattga aaatccgcgc
     1921 ttggttcgcg gtttggatta ttacaatcag acggtttttg agtggacgac cgacaaactc
     1981 ggcgcgcagg cgactgtgtg cggcggcggc cgttacgatg gtttgattga ggaacttggc
     2041 ggtaagcctg cgccgtccat cggctttgca atgggcatcg aacggctgct gcttttggtg
     2101 ggcgaatacg gctcgctgga agtaaacgcc gcgcctgatg tctatgcaat gcaccaaggc
     2161 gaaggggcgg acttgcaggt gatgaaatac gcgcaagcct tgcgcgcgca aggtttcaat
     2221 gtaatgcagc attccggcta tcaaagcctg aaagcacaaa tgaaaaaagc cgacaacagc
     2281 ggcgcgcgct ttgccctgat tgtggcgcaa gacgaactgg cgaacggtac ggttacgctc
     2341 aaagacatga acggcgcaca cggtcagcaa actgtcgccg ccgaggattt gacaaacact
     2401 ttacaacaat ggaagaacgc ataaatggca gcccatttgg aagaacaaca agagttggac
     2461 aactttaaat atttttggaa aaccacgggc aaatggctgt ttgccctgct gattttggca
     2521 gcactcggct acttgggata cacggtttac caaaaccgtg cggcttccca aaatcaggaa
     2581 gccgctgcca tattggcaga aatcgtagaa aaggcgcaaa gcaaagcccc gcaaagcgaa
     2641 atcaatgccg aattgaccaa actccaacaa agctacccgc attccatttc cgccgcccaa
     2701 gccacgctga tggcggcggc aaccgaattt gacgcacagc gttacgatgt tgccgaaggc
     2761 catttgaaat gggtgttgtc caaccaaaaa gacagcctga ttcaggcgtt ggcggcgcag
     2821 cgtctgggcg ttgtgttgtt gcaacaaaaa aaatacgatg ccgcgcttgc cgcgctcgac
     2881 acgccggttg aagcggactt cgcccccctg ctgatggaaa ccaaaggcga tgtctatgcc
     2941 gcacagggaa aaagccagga agccttaaaa aactacggac aggctttaga aaaaatgcct
     3001 caagattctg tcggtcgcga attggttcaa atgaaacttg attcgctgaa ataaatgccg
     3061 tctgaaatgc tgtccattat ttatcagacg gcattttacc ctccctcttt atggcctctt
     3121 gccgtgaaga acctatttac cgtgtccaac ggacacctgc tcgtgttcag ttctcccgaa
     3181 cggcacttga cacagaaaga cccaataatg aaacccacca tcgcgcttgt cggccgccct
     3241 aacgtcggca aatctacttt attcaaccgt ttgacgcgca ccaaagacgc actcgtacac
     3301 gacctgcccg gcctgacccg cgaccgccat tacggacacg gcaaagtcgg cagcaaacct
     3361 tatttggtca tcgataccgg aggtttcgag ccggttgtgg acagcggcat tttgcacgaa
     3421 atggcaaaac aaaccttgca ggctgtcgat gaagctgatg cggttgtgtt tttggtggac
     3481 ggccgtaccg gcctgacccc gcaagacaaa atcattgccg accgcctgcg ccaaagcccg
     3541 cgccctgttt atctggcggt aaacaaaggc gaaggcggca acagggccgt gcttgccgcc
     3601 gagttctacg aacttgcttt gggcgaccct tatgttattt ccggtgcgca cggcgacggc
     3661 gtgtattacc tgattgaaga aattttagaa aacttccccg agccggaagt cgaagaagcc
     3721 gatgcaaaac atcccgtttt tgccgtcatc ggtcgtccga acgtcggcaa atccacgctg
     3781 gtaaacgcca ttctcggcga agagcgcgtg attgccttcg atatggccgg cacgacgcgc
     3841 gacagtatcc atatcgattt cgagcgcgaa ggcaaaccgt ttaccatcat cgataccgca
     3901 ggtgtgcgcc gtcgcggcaa agtggatgaa gcagtggaaa aattctccgt tatcaaagcg
     3961 atgcaggcgg ttgaagcggc aaacgtcgct gttttggtat tggacgcgca gcaggacatc
     4021 gccgaccaag atgcgacgat tgcaggtttc gctttggagg caggccgtgc tttggtggtg
     4081 gcggtcaata aatgggacgg catcagcgaa gaacgtcgcg agcaagtgaa acgcgatatc
     4141 aaccgcaaac tgtatttcct cgattttgcc aagttccact ttatttccgc attgaaagag
     4201 cgcggtatag acggtttgtt tgacagcatt caggctgcct acaacgcggc gatgattaag
     4261 atgccgacgc cgaaaatcac gcgcgtcctg caaagcgcaa tcgagcgtca gcaaccgccg
     4321 cgtgccggct tggtgcgtcc gaaaatgcgt tatgcccacc aaggcggcat gaaccctccc
     4381 gtaattgtgg tacacggcaa ttcgctgcac gcgatttccg acagctatac gcgctatctg
     4441 acccaaacgt tccgcaaagc cttcaacctg caaggcacgc cgttgcggat tcaatacaat
     4501 gtttcggaaa acccgtatga aaacgccgac gacaaaccga agaaaaaacc gctgcgccgc
     4561 gtcagcctga gcaaccgtat tgagaaacgc gaaggccgca aagaagagaa aaaccgcttc
     4621 aagaagaaaa ccaaagtcag tgtgaaaaaa caattcagca aataattccc gacatttcaa
     4681 acaacaggaa acattatgtg gttcaagcag attagttttt atccgctcaa caaagaaaag
     4741 ctgcctgagg cggacgtact tgccgacaaa cttgctgaag ctgaatttac ccattgccaa
     4801 ggcttagact ggttcagcga aggctttacc gcaccggttt cattctcccc ggaactcgtt
     4861 tttcctgccg actttacctt gcgcgtcgcc ctgaaaaaag aggaaaaagt cctgcctgcc
     4921 ggcgtcatcc gcgatatttt ggaagagaag gtagcggaaa tccaaaacaa tgaagcccgc
     4981 aatgtcggtc gcaaagaaaa acaagagctt aaagagcaaa ttacagacga cctgctgccc
     5041 cgagcgttta cccgcagcag ccgtacagaa gcggtgttta acacccgcca cggctacctg
     5101 ctcgtcaata acgcggcttc cgccaaagca gaaaacatcc tgaccaagct gcgcgaagct
     5161 ttgggcggtt tggaagcctc actgccgaat accaaacaat cgccctcttc cctgatgacc
     5221 ggctggctgt tgcaagggca ttgcgaaggc ggttttgaat tggacagcga ttgcgaactc
     5281 aaaggcacgg gcgatattgt tcccgtcgtc aaagtatcca aacaagattt aaccgccgac
     5341 gaagtggttc aacacgtcaa aaacggtaaa accgttaccc aactgggctt ggtgtggcgc
     5401 gaacaaattg cctttatcct cacgcaagac ttcacactca agcgcatcca atacctcgac
     5461 gtattgcagg aagaagccga aagcaacggc gacgatgccg ccggtctcgc cttcgcctcg
     5521 caaatcctga tggcggaatc cgtcagcatc atgttggaag agctggtttc ctatttgggc
     5581 ggctggcaag attgattttt cagacggctt ttataccgtc agccaaaatc caatggaata
     5641 acccttatgc ttaaacatct cgcattccta ctgcccgcca tgatgttcgc cctccccgcc
     5701 gcgtccgccg ttctgacttc ctatcaagaa cccggctgca cctacgaagg cgatgtcggc
     5761 aaagacggta aacccgccgg caaaggcaca tggcgctgcc aagacgggcg caactatacc
     5821 ggttcgttta aaaatggcaa attcgacgga caaggcgttt ataccgttgc cgccaaccgc
     5881 gaaatattta tcgaaccgtt caattccgac agtaccaaat tccgcaacat ggtactctcg
     5941 ggcacattca aaaaaggctt ggcacacggc agatttaccg tctcgcaaaa cggcgaaacc
     6001 ctcttcatta tgaaatgcga aaacggcatg attaaagaag tgaagctgcc caaaaacaaa
     6061 taagccccgt tttcagacgg catcccccaa tgctttgtcc gaacactgca agaaacaagg
     6121 aaaacattat gagcatcaag tccgacaaat ggatacgccg aatgagcgaa gaattcggca
     6181 tgatcgaccc tttcgagccg aaccaaatca aagaagccga cggcaaacgc atcatctcct
     6241 acggtacgtc cagctacggc tacgacatcc gctgcgcaaa cgaatttaaa atcttcacca
     6301 acatcaacag caccatcgtc gatcccaaaa acttcgaccc gaaaaacttc gttaccgttg
     6361 aagacgactg ctgcatcatc ccgcccaatt ccttcgcact ggcgcgcacg gtcgaatatt
     6421 tccgcatccc gcgcaacgtc ctgaccgtct gcttgggcaa atccacctac gcccgctgcg
     6481 gcattatcgt caacgttacc ccgttcgaac cggaatggga aggctacgtt accctcgaat
     6541 tttccaacac cacccccctg cccgccaaaa tctacgcagg cgaaggcgtg gcgcaagtcc
     6601 tcttcttcga gagcgacgaa atctgcgaaa cctcatacaa agaccgcaac ggcaaatata
     6661 tggggcaaac cggcgttacc ctgcccaaag cctgaaatat aaatgccgtc tgaaccctta
     6721 tcgtcgggtt tcagacggca tctacctgtt tatccgtggt tagaactgaa ccgtcccatt
     6781 gacgctgatg ctgatttcct ccacaccctg cgcggcagaa tccgcaccct ccatattgac
     6841 gcttgccgcc atcggcatgg cacgaagcat ttttgcctga gcagctcccc ctcccgcgat
     6901 atggctgccg atgtgtccca aattcaattt gacgatttta taaccggacg cacccaaaac
     6961 gcccgccaac ttttcggcac gcgccttgaa acgcaaaacg gcatccttgc tgacctgatc
     7021 gatgacctcg ttgcggcgtt cgcgcgacac atggaaatcc gtatattcca acgcggcatc
     7081 tgcttgaata tcggcaataa aacggtttaa ctcatcaaaa tctctacctt cgaccttaaa
     7141 ttccgcacgc tcctcccagc ctgtttgaat gcgtctgccg ttggtatatt gatagcgcgg
     7201 catcgcactg cgcgatacca attcggtttt aaagctgccg ttttttgatt ttctggtgaa
     7261 attgttgaat tttttaacaa actcggcatt gacggcattt ttgtcccgtc cttccgccgt
     7321 cacttggaaa cgtgcggaca ttgtatcctg aaccgcctcg acacccgccg attcagaaaa
     7381 ttcgacaata ttgtaattca atgcttcagc cgccgtcgga aaagataccg ccagcaggga
     7441 agccgccaaa atagaacgca acatatccgt atccttactt ataaaaccgc caatctagca
     7501 tatcggcgcg ctaaattgca ttagcgtaag gcaacaattt aaatattcgg tctgcgtctg
     7561 tcttataata accgcctttc cgatacggaa tcccactatg cccgaaccct tgcacgtcct
     7621 cgtcatcccc tcatggtatc cgcaatccga acaggatgtg gacgggattt ttttcaaaat
     7681 caggcactgg cattgcagag gaaaggcatc aaaaccgccg tgcttgcacc gatgttccgc
     7741 tacttgcgga aagaaacagc aagcatcctg accggcctta cggttttgca aaatatcggc
     7801 aaagcggttt ggacatctat gcatggcacg gcatatattt cttcccccgc tttccgttca
     7861 tcgacatcga ctgcatccgc tgggtgtgtg cgggtttgaa agctttcaaa cactacatcc
     7921 gcgaaaacgg gctgcccgac ctgattcacg cccaccgcat gaactatgcc ggcatactcg
     7981 cccaaaaaat ttcccaaaaa tacggcatcc cctatgtcgt cacggaacac agcagcacca
     8041 tcacgcgcgg tctgatccgt ccccaccaat ggcagccgat ggaaaaagca gcggcgcacg
     8101 cctccgcact gctcgctgtc agccgccatt tcgcacacgt cctgcaacac aaatacggct
     8161 gcgaatggca gtacctcccc aacatactgg gcggaatatt caaccgaccg tttgaacaaa
     8221 gagaaaaaaa caacaaaccg catttcgtgt tctgcaccgt ctcgcacctt cgccgtctca
     8281 aaggacacga tgtcctgctc actgcctttg cccgggcgtt ggcacaatgc ccgcaactgc
     8341 gtctaaacat cggcggcagc ggacaggaag aacagcggct gaaacagcag gcggcagact
     8401 tgggcatcac ccatgccgtt acatttttgg gcgcattgca gcccgaagca gtcttggatt
     8461 tgatgaggaa cagcgacgca ttcgtccttg ccagccgcac cgaaaccttc ggcgtggtct
     8521 atatcgaagc actgtcccaa ggattgcccg tcattgcaac acgctgcggc ggtgcggaat
     8581 ctattgtttc agacggcaac ggatatttgg ttcctgttga cgacgacgat gcccttgccg
     8641 acgcactcat caaaatgtat gaacaccact ctgattttga acctgcccga cttagggaaa
     8701 actgtctgaa cgaatttggc gaaaatgccg ttataggcag gttgatcggc attttccgac
     8761 aggcaatcgc agaatacggt aagaaaatac cgg
//
LOCUS       DAXHPG010000079         8756 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXHPG010000079 DAXHPG010000000
VERSION     DAXHPG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.079"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..247)
                     /locus_tag="ACFM6F_001321"
     CDS             complement(<1..247)
                     /locus_tag="ACFM6F_001321"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6164724.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYID"
     gene            486..1385
                     /gene="crgA"
                     /locus_tag="ACFM6F_001322"
     CDS             486..1385
                     /gene="crgA"
                     /locus_tag="ACFM6F_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC6164725.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSAEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1612..4827)
                     /gene="carB"
                     /locus_tag="ACFM6F_001323"
     CDS             complement(1612..4827)
                     /gene="carB"
                     /locus_tag="ACFM6F_001323"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC6164726.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLVKEEKSVSDGILSDLDFAALRRLKRKGFSDKRIAQLLGVKEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRS"
     gene            complement(4856..5497)
                     /locus_tag="ACFM6F_001324"
     CDS             complement(4856..5497)
                     /locus_tag="ACFM6F_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFC6164727.1"
                     /translation="MSIPINFNNLKYLLNDMRNKNRIIEAFPFNYNQRQYAVILTRYK
                     PDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFINFFKINVQAGAAKIR
                     EIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIYCYDVRRNGKDKAGKP
                     NRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDAEIIREVTNRQ"
     gene            5659..6057
                     /locus_tag="ACFM6F_001325"
     CDS             5659..6057
                     /locus_tag="ACFM6F_001325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164728.1"
                     /translation="MRYVCTRSPFSLQPQPTACVPCGTLSADEFKGRLKKRSQRLFHL
                     PCDKGCLKHSRCRCFGHSFSDDLQRLNLLQPTPSPVGEGWGEGILRISATFPNTLTAK
                     TQALWLVALSLTLSHGREDWAVVGAKVSVN"
     gene            complement(6165..6554)
                     /locus_tag="ACFM6F_001326"
     CDS             complement(6165..6554)
                     /locus_tag="ACFM6F_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC6164729.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7128..7253)
                     /locus_tag="ACFM6F_001327"
                     /pseudo
     CDS             complement(7128..7253)
                     /locus_tag="ACFM6F_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7299..8432)
                     /gene="carA"
                     /locus_tag="ACFM6F_001328"
     CDS             complement(7299..8432)
                     /gene="carA"
                     /locus_tag="ACFM6F_001328"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC6164730.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNQTVAIADIDTRRLTTLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         1948 a         2248 c         2288 g         2272 t
ORIGIN      
        1 cgtctatgta ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa
       61 tcacagcatc catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa
      121 tcacggtttc ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa
      181 gcgtgcggtt taacccgccg tgagaatgtc cgaacgcttt gccgccgtct aataataaaa
      241 tattcatttg ttacctcgtt tgtgaattga tgaatgtatt gtgccgtttt actttataat
      301 ttaaaagtgc aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgttccaac
      361 acacgggatg gcacataaag caccgccctg tgtgccgtcc tgattcggaa ggggttacac
      421 ccctcccaaa taaagtctga tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa
      481 acacgatgaa aaccaattca gaagaactga ccgtatttgt tcaagtggtg gaaagcggca
      541 gcttcagccg tgcggcggag cagttggcga tggcaaattc tgccgtaagc cgcatcgtca
      601 aacggctgga ggaaaagttg ggtgtgaacc tgctcaaccg caccacgcgg caactcagtc
      661 tgacggaaga aggcgcacaa tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag
      721 cggcggaaac cgaaatgctg gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt
      781 ccgcgatgcc gatggtgctg catctgctgg cgccgctggc agcaaaattc aacgaacgct
      841 atccgcatat ccgactttcg ctcgtttctt ccgaaggcta tatcaatctg attgaacgca
      901 aagtcgatat tgccttacgg gccggagaat tggacgattc cgggctgcgt gcacgccatc
      961 tgtttgacag ccgcttccgc gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc
     1021 cgcaatctgc agaagagctt gccggtcacc aatgtttagg cttcaccgaa cccggttctc
     1081 taaatacatg ggcggtttta gatgcgcagg gaaatcccta taagatttca ccgcacttta
     1141 ccgccagcag cggtgaaatc ttacgctcgt tgtgcctttc aggttgcggt attgcttgct
     1201 tatcagattt tttggttgac aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg
     1261 aacaaacctc caataaaacg caccccttta atgctgttta ttacagcgat aaagccgtca
     1321 acctccgctt gcgcgtattt ttggattttt tagtggagga actgggaaac aatctctgtg
     1381 gataattcta taaaaacctg ccataaaatt gtttttactc acttaataac tttgagaaaa
     1441 aataaataag atgatgtata gtcggcggca atggcagtct atatggggag cgtgataggg
     1501 ctgttcctta tgtacggctt gtgtgtggca acatcggctt tccaaatgac aaaatgccgt
     1561 ctgacaccgt aaaaaaaggt ttcagacggc attttcaacc cgattcaggc atcagctgcg
     1621 gtttttcaaa cgcccgtgca gttcttgaac gctgtacacg cccaagtggt cgcggctttt
     1681 cgcgccttcg ctcatcgctt cgccgcccgc agtagtggtg tattgcggca cacgttgctg
     1741 caatgcgctt tggcggatga tgtggctgtc ggacacggat tgcggatcgc tggaaacggt
     1801 attcacgacc agtgcgattt cgccgttttt cagcgcgtcg ccgatgtgcg ggcggccttc
     1861 ggggacttta ttgatggttt gtacaatcag cccgtgttcg gtcaggtatt gcgccgtgcc
     1921 gcgcgtggcg cagatgccgt agcctaaggc ttggaagttt ttggcggttt taatgacgcg
     1981 ttctttgtct tcttcgcgca cggagaggaa gattttgccg gtcgggttga ggcgttcgcc
     2041 cgcgccgagt tgggctttgt agtaggcttc gccaaagctt gcgcccacgc ccatgacttc
     2101 gccggtggag cgcatttccg gtcccaaaat cgtatccacg cccgggaatt tgatgaatgg
     2161 gaacacggct tctttaacgg catagaaatc ggggacaact tctttttcca cgccttgttc
     2221 tttcagggaa atgcctgcca tgcagcgcgc gccgactttg gcgagcggca cgccggtggc
     2281 tttggagacg aagggcacgg tgcggctggc gcgcgggttc acttccaata cgaacaccac
     2341 gccgtcttgt acggcaaact gcacgttcat cagtccgacc acgcccagcg cgtacgccat
     2401 cgctttagtt tggcggcgga tttcgtcttg gatttcttcg cttaaggagt agggcggcag
     2461 cgagcagccg gagtcgccgg agtggatgcc cgcctgttcg acgtgctgca tgatgccgcc
     2521 gataaccacg tctttgccgt ctgaaacgca gtccacatcc acttcaatcg cgttgttgag
     2581 gaagaaatcg agcaacacgg ggctgtcttc ggaaacctgc acggcttcgc gcatgtattt
     2641 ttgcaaggct tcggcagagt ggacaatctg catcgcgcgt ccgcccaaaa cataagacgg
     2701 gcgcacgacc agcggatagc cgatttcttc ggctttgacg agtgcttctt cttcgttgtg
     2761 ggcgatgcgg ttgggcggtt ggcgcagacc taagtcgttc aacacttttt ggaagcgttc
     2821 gcggtcttcg gcggcgtcga tgctgtcggc ggacgtgccg atgatgttta cgccgttttc
     2881 aaccagcgcg ttggcgagtt tgagcggggt ttgaccgccg taatgcacaa tcacgcccca
     2941 cgggttttcg gtgcggacga tttccaacac gtcttccaat gtcagcggct cgaaatagag
     3001 gcggtcgctg gtgtcgaagt cggtggacac ggtttctggg ttgcagttga ccataatcgt
     3061 ttcaaaaccg gattcgcgca gggcgagcgc ggcgtgaacg cagcagtagt caaactcgat
     3121 gccctgaccg atgcggttcg ggccgccgcc gagaatcatc acttttttac ggtcggaagg
     3181 acgggattcg cattcttctt cgtaagtgga gtaaagatag gcggtttcgg tggcgaactc
     3241 ggcggcgcag gtgtcaacgc gtttgtaaac cggatgcagc ttcagcgcgt agcggtgttc
     3301 gcgcacttct ttttctttta cgcccaaaag ctgcgcgatg cgtttgtcgg agaagccttt
     3361 gcgtttcaga cggcgtaggg cggcgaaatc caaatcactc aaaatgccgt ctgaaaccga
     3421 tttttcttct ttcaccaagt cttcgatttg cgccaagaac caagggtcga tggcgcagat
     3481 ttcgtggatt tcttccggcg tgaagcccgc gcggaacgcg tctgccacaa acagcatgcg
     3541 ctcggggccg gggtttgcca gttcgcggcg gatttccgct ttgtcggagc ttctcggatt
     3601 gaagccgcac aagcctgttt ccaagccgcg cagggctttt tggaaacttt cctgaatcgt
     3661 gcgtcccatc gccatcactt cgcccaccga tttcatctgc gtggtcaggc ggtcgtctgc
     3721 ggcagggaat ttttcaaacg caaaacgcgg gattttggtt accacatagt cgatggacgg
     3781 ctcgaatgac gcgggggttt tgccgccggt gatgtcgttg cgcaactcgt ccagcgtaaa
     3841 gccgaccgcc agtttcgccg ccaccttcgc aatcgggaag cccgtcgctt tggaagccag
     3901 cgcggatgaa cggctcacgc gcgggttcat ctcaatcaca atcatctcgc cgttttcagg
     3961 gttcaccgca aactgcacgt ttgagccacc cgtgtccacg ccgatttcgc gcaatactgc
     4021 caacgaagca ttacgcatga tttggtattc tttgtctgtc aatgtttgcg ccggcgcaac
     4081 cgtaatcgag tcgcccgtat gcacgcccat cgggtcgaag ttttcaatcg agcaaatgat
     4141 gatgcagtta tcgttcttat cgcgcaccac ctccatctcg tactctttcc agccgaggac
     4201 ggactgctca atcagcagct cgtgcgtggg cgacgcatcg aaaccgcgtt cgcaaatcgc
     4261 caaaaactcg tctttattgt aggcaatgcc gccgcccgaa ccgcccatcg tgaaagacgg
     4321 acgaatcagc gtcgggaagc cgacctgttc ttgcgccgcc aaggcttcgt tcatcgtgtg
     4381 gcagacaaaa gatttcgggc aagacaaacc gattttttcc atcgcttctt taaagcggcc
     4441 acggtcttcc gccttgtcaa tcgcgtcttc tgtcgcgccg attaactcga cgttgtattt
     4501 cgccagcacg ccgttgcgcg ccaaatccag cgcacagttc agcgcggtct ggccgcccat
     4561 cgtgggcaga atcgcatcgg gccgctcttt ggcgataatc ttctccaccg tctgccacat
     4621 aatcggctcg atgtaggtaa catccgccat ttcagggtcg gtcatgatgg tggcggggtt
     4681 ggagtttacc aaaatgactt tatagccttc ttcacgcaaa gccttgcagg cctgtgcgcc
     4741 cgaatagtca aattcgcagg cctgaccgat aacgataggg ccggcgccga tgataaggat
     4801 ggattttagg tcggtacgtt tgggcatggt gggtctttct tttgaagaat ttggattatt
     4861 gacgattggt aacttctcta attatttctg catctgtttt ttcatcgctt ggattatctg
     4921 aaaagaaaaa actgtaatta ctatcggctt taaatttttc gtatagctct gggcgcaaaa
     4981 tttttgcttt ttcactattt tccacgctcc tgcgattagg cttgccagcc ttatctttcc
     5041 catttctacg gacatcatag caataaatag tgttaggaga attaggttct aaacgagtag
     5101 ctacaatctt tttataaatt atgtctaaat cttttttagt ttgtgttgga atgaaatctg
     5161 caaaaagatt actaaaactc tgaaaaattt ctctgatttt cgcagcacca gcctgaacat
     5221 taattttaaa aaaattaata aaatcagtag cacttttaaa atgaacttca taaaaatcag
     5281 catacgcaaa tattgaattt tcactattca aattaaaaaa ctctaatttt gcttgtgcat
     5341 aatcatctgg tctaggttca tcaggtttat acctagtcaa aataacggcg tattgccttt
     5401 gattataatt aaaaggaaat gcttcaatta ttctattttt gtttctcata tcattaagca
     5461 aatactttaa gttattgaaa ttaataggta ttgacatatt taaactcctg cgaatatgtt
     5521 gcaataaacg tagcaatcgt aggttgggtt gaaaacccaa cacaaaccgc ttaattgtct
     5581 aaaatgttgg gtttgctaac ctaacttgta tatcgaaacc cccgtatcgt agcaagccgt
     5641 agcccgtatg tagggtatgt gcggtacgta tgcacgcgtt ctccattttc cctgcaaccc
     5701 caacccaccg cgtgcgtgcc ttgcggcaca ctctctgccg atgagttcaa aggtcgtctg
     5761 aaaaaacgtt cgcaacgttt gttccacctg ccttgcgata aaggctgcct gaaacattcc
     5821 cgctgtcgat gtttcgggca ttctttttca gacgacctgc aacgcctgaa tctgctgcaa
     5881 cctaccccct cccccgtggg ggagggctgg ggagagggca ttctccgaat ttcggcaacc
     5941 tttcctaaca ctctaaccgc caagacacaa gccttgtggc ttgttgccct ctctctaact
     6001 ctctcccacg ggagagagga ctgggcggtt gttggggcta aggtttctgt aaactaatca
     6061 ggttatgcag gctgcttttt gtttttcaga cggcctttat aaatgctcaa ttcagtcggc
     6121 tttatcctat tattttcaat aatccaatca aaatcagcca tctgctatcg tgcagcttgc
     6181 ttttccaatt cttgcaacac gagcaggatt tctgtcagca catcgtttgt ctgctgtaaa
     6241 atctcgtgat tccaaaaccg cagtacggta aagcccaagc tgttgagata tgccgtccgc
     6301 gcgtggtcgt atgcggcttg ttctgtgtgc tgccctccgt ctgcttcgac aatcaacttg
     6361 ggcgttacgc acataaaatc aacaatatag tttcccatcg gctgctggcg gcggaattta
     6421 tagccgttca gacgacctgc ccgcaggtgc tgccacaatt ttgcttccgc cccgctcatt
     6481 tcttggcgca tggctttggc gcgttggcgc aagatggggt tgtcggtggt caagagtttc
     6541 tcgtgcgggt tcattttgtt gtcgtgctgt ttgctgccct ctctctaact ctctcccacg
     6601 gggagagagg acggggcggt tgttggggtt aaaggggctg taaggaaaac aagtcgttca
     6661 ggccactttt tacttttcag acgacctgca atcaacggtt gtaaatccaa gttgcttttc
     6721 aaaccgcctg taaccgcaaa acttgctgca acctaccccc tcccccgtgg gggagggctg
     6781 gggagagggc attcttcgaa tttcggcaac cttccccaac accctaaccg cccaaacaca
     6841 agccttgctg tttgttgccc tctctctaac gctttcccac agggagacag gacggggcgg
     6901 ctgtagtgat taagggtttt gcaaggaaaa caaggaaaac aagttgtaca ggctgttttt
     6961 ttatttttca agcgacctga aatcaacgct tgtaaatcca agtcgctttt caaactgcct
     7021 gcaaccgcaa aatctgctgc aacctacccc ctcccccgtg ggggagggct ggggagaggg
     7081 cattttccaa gttgcggcaa tctttcccaa atcccttagt cttctaaaat acaagcctta
     7141 cggttgttgc caaatgaaac ttcttccata cccaaaactt gcgtgtaaaa cgcaatggtt
     7201 cggtcaatgt cggcaacggt cagtaccaga tggtcgagtg cgctaatctt catttcccgc
     7261 tttctttttc aggcggcggc agtgccgtct gaaagccatt atgcccgttt tgccgctttc
     7321 atattgccaa tgaatttgtc aaacaaatag ccgacatctt gcgggccggg gctggcttcg
     7381 gggtggcctt ggaagcagaa cacaggtttg tcggtcagct cgatgccttg caaagtgttg
     7441 tcaaacaagg atttgtgggt aatgcgtgcg ttagcgggca gggtgtcggc atcaacggca
     7501 aaaccgtggt tttggctggt gatgacgact ttgccgctgt ccaaatcttg cacagggtgg
     7561 ttcgcaccgt ggtggctgaa gcgcattttc agggttttcg cgccgatagc gaggctgatg
     7621 agctggtgtc ccaagcagat gccgaaaatc ggtttgccgc tttccatcag tttttgcacg
     7681 gcttcgatgg cgtaggtgca aggctcgggg tcgccggggc cgttggacag gaacacgccg
     7741 tcgggattga gtgccaacac gtcttgtgcg ctcgtttggg cggggacgac ggtcaggcgg
     7801 cagccgcgcg aggcgagcat acgcaggatg ttggttttca cgccgaaatc gtaggcgacg
     7861 acgtggtaag gctgttcgtc aggggtaacg aaacccttgc ccaatgccca ttcaccttcc
     7921 gtccattcgt aagtttccgt gcaggaaact tcttttgcca agtctttgcc gaccatgctg
     7981 ccgaacgcgg cgatgagttc ttgcgctttt tcgaccgtag cgtccgcgcc ggtcagaatc
     8041 gcaccgcctt gcgcgccttt ttcacgcaac agcgtggtca ggcggcgggt gtcgatgtcg
     8101 gcgatggcaa cggtttggtt gcgcaccaga tagtcgtgca ggctttcgga ggcgcggaag
     8161 ttgctgtgca agagcggcag gtcgcggata atcaaaccgg cggcgtaaac gctgcggctt
     8221 tcttcatctt cggcgttggt accggtattg ccgatgtgtg ggtaggtgag ggtaacgatt
     8281 tgtttgcagt aggacgggtc ggtcaggatt tcctgatagc cagtcatcga agtattgaac
     8341 acgacttcgc cggaagtcga accttcgtaa ccgattgatg tgccgtggaa tacgctgccg
     8401 tcagcgagga cgaggagggc gggggtgctc atgatgggaa tcctgttttt aataaaattc
     8461 ttgacaatgc cgtctgaagg gacttcggca gggggcatcc ggtcggaaaa atccggtcaa
     8521 aaaaaaacac gccgcgcaag atagacgcgt aacgtgcttt tcgggcaagt gcctataccc
     8581 tgaaagcagc atattttaag acgaaacata cccgatttca agcgcaaacg cggcaggcgg
     8641 gctgatatgt tcgcgcgaaa acggaaatcc gtggcgtaaa accgcccgct ccgcgcccac
     8701 tatgcccgtt acgcatttcc ccacgtccgt ccggcgaaac tatgggaata cccgaa
//
LOCUS       DAXHPG010000080         8693 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXHPG010000080 DAXHPG010000000
VERSION     DAXHPG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.080"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            192..1727
                     /locus_tag="ACFM6F_001329"
     CDS             192..1727
                     /locus_tag="ACFM6F_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6164731.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1724..1816
                     /locus_tag="ACFM6F_001330"
     CDS             1724..1816
                     /locus_tag="ACFM6F_001330"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC6164732.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1852..3096)
                     /gene="lysA"
                     /locus_tag="ACFM6F_001331"
     CDS             complement(1852..3096)
                     /gene="lysA"
                     /locus_tag="ACFM6F_001331"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC6164733.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDATKTIFSGVGKNEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTASVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDENVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3107..3277)
                     /locus_tag="ACFM6F_001332"
     CDS             complement(3107..3277)
                     /locus_tag="ACFM6F_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6164734.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDTARFGAIQTGLQL
                     QNKPQSAPQTRK"
     gene            3348..3671
                     /gene="cyaY"
                     /locus_tag="ACFM6F_001333"
     CDS             3348..3671
                     /gene="cyaY"
                     /locus_tag="ACFM6F_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC6164735.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3698..4714
                     /locus_tag="ACFM6F_001334"
     CDS             3698..4714
                     /locus_tag="ACFM6F_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFC6164736.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAESVTKAESHKVSVAIAIV
                     VIFGTLAIFTYPLFYTWSQDLINAHQFGIYVGSSVHEVAQVYAIGENINPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSDGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4740..5159
                     /locus_tag="ACFM6F_001335"
     CDS             4740..5159
                     /locus_tag="ACFM6F_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC6164737.1"
                     /translation="MAQLPLYRTAEIENFTVGTPEVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAQGEWPKVTDRVLDDELQDKILSRKPT"
     gene            5181..5687
                     /gene="luxS"
                     /locus_tag="ACFM6F_001336"
     CDS             5181..5687
                     /gene="luxS"
                     /locus_tag="ACFM6F_001336"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC6164738.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVGNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLAHKVAVNKNEELT
                     LDEGLLNA"
     gene            5832..8621
                     /gene="polA"
                     /locus_tag="ACFM6F_001337"
     CDS             5832..8621
                     /gene="polA"
                     /locus_tag="ACFM6F_001337"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC6164739.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMAQLTAPDGAPTGAMYGVLNML
                     RRLRADYVHDYCAVVFDAKGKNFRHEMFPDYKATRPPMPDDLRPQAEALPDLVRLIGW
                     PVLVIPQVEADDVIGTLAAMAGEAGWNVVVSTGDKDMAQLVNERVTLVNTMSGETLDI
                     EGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAAE
                     IKGKVGENLRAALQRLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLVVDFKR
                     WGFRTWLKEAESRMHEAADGDLFGSDSIGEQAALNAEVPSEKQPELAPTPEKLDYQAV
                     TTEAQLAALLDKLSQADTIGIDTETTSLDAMNASLVGISIAFQAGEAIYIPVGHSLTA
                     APEQIDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYII
                     ESHLGHGLDELSERWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALRL
                     EAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELSRQSAELGAELMKLEQ
                     EAYAVAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPLP
                     KIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPIR
                     TAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLITAFQNGEDVHRRTAAE
                     VFGIAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNISAKTFIDRYFARYPGV
                     AEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDLI
                     KRAMIDVRNCLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDVP
                     LVAEVGVGENWEEAH"
BASE COUNT         2033 a         2277 c         2293 g         2090 t
ORIGIN      
        1 aaaatttttc gttttcaagc cttcaccgct tgccatcagc gttaaatttt tttacgataa
       61 gcacatagat tgtaaacaat cggccacaag ccggtttgtt ttttcagaag acattatccc
      121 tgtcagacgc tatttctata tatttcgcct ataatggctt gtttttaata aataattcaa
      181 gaggtatcaa cgtgtctgat tccaagacga aagaacgcgc cacattcggc acgcgccgcg
      241 cgtttatgat tgccgccatc gggtccgccg tcggcttggg caatatttgg cgtttcccct
      301 atattgcttt tgaaaacggc ggcggcgcgt tcatcctgcc ctatctggtc gcgcttctga
      361 cggcgggcat cccgctgctg ctgctcgatt atgccatcgg ccaccgttac cgtggttctg
      421 cgcccttggc tttccgccgc ctcggacgat ggtttgagcc ggtcggctgg tggaacgtga
      481 tgaccaatat cgtcatctgc atctattacg cggtaattat cggttgggcg gcaagctata
      541 cctattattc ggtcaacgcc gcctggggtg cggatccgca gggttttttc tttaaggact
      601 tcctgcaaat ggcgggcccg gaagccttgg gtttggattt tgtcggcaaa gtcgccggtc
      661 ctttggcggg cgtgtgggtt tttaccgccg ccattatggc gttgggcgtg caaaagggcg
      721 tggcgcgcgc ctcgtcgttc tttatgccgc tgcttttggt gatgtttttg attatggtcg
      781 gcatttcact aaccctgccg ggtgcggcaa agggcttgga cgcattgttt acgcccgact
      841 ggtcgaaact cgccgattcc aaggtctggg tggcggcata cgggcagatt ttcttttcgc
      901 tttccatctg cttcggcatt atggttacct attcttctta tttgaagaaa aaaaccgact
      961 tgggcggaac ggggctggtg gtcggttttg ccaacagcag ctttgaactg ctcgcgggca
     1021 tcggcgtgtt tgccgcattg ggctttatgg cgcaggcggg cggtaaggcg gtcaacgagg
     1081 ttgcctcagg cggcatcggt ttggcgttta tcgcctttcc gaccattatc aaccaggcac
     1141 cgatgggctg gctgatcggc atattgtttt tcggttcgct ggtattcgcc ggcgttacgt
     1201 cgatgatttc catccttgaa gtgattgtgg cggcgattca ggacaagctg aacatcgggc
     1261 gcgtcaacgc cacgctgctg gtctgcattc cgatgggcat tgtttccacg ctgctgttcg
     1321 gtacggcgac ggggctgccg gttttggacg tgatggacaa attcgtcaac acctacggca
     1381 ttgttgccgc cggctttgtt tatgttgccg ccatcatcat cagcggcagg ctgccggaat
     1441 tacgcaagca cctgaacgct ttgtcctcca tccgcatcgg cggcttgtgg acggtctgcg
     1501 tcgtggttac cgtcgtgatg ctcggctata tgctgtttaa agataccagc ggcctgatgg
     1561 agaaaaatta cgaaggttat ccggatggtt tcctcagtat tttcggctgg gggatgtcgg
     1621 cggcgttggt cgtgttcggg ctgctgctgt cgttgctgcc ttggaaacac ggtcaggatt
     1681 tcaacgtcaa agacgaacac gaacatgaac aaggagaaga aaaatgagta cttccgccat
     1741 tgtgatgatg attgtctcaa tcgtgataat ctggggaggg ctgctgcttt ccctgttaag
     1801 gctgccgaac gagtaagcct ttagagcgtt aaaaatgccg tctgaaccgc ttcagacggc
     1861 attttgatgt tcggcttgca ggcaggcgag ttcgtttgcc atttgctgtt ccaaggtttc
     1921 gcgccggcgg atgagtcggt attcgttgcc gtccaccaac acctctgccg cacggttgcg
     1981 cgcgttgtaa ttgctcgcca tactggctcc gtatgcgccc gcgctgcgga taagcagcaa
     2041 atcgccttct tcgcaggcga tggtgcggtc tttgccgagg aagtcgccgg tttcgcaaat
     2101 cggaccgacg atgttggcgg tcagaggctc aatgtctttg gtttcgaccg cctcgatgtg
     2161 atgataggca tcataaagcg ccgggcgcat caaatcgttc atcgccgcat cgaccatcac
     2221 aaagtttttc tcttcgccgt atttgacgaa ttcgacgcgt gtcagcagcg aacctgcgtt
     2281 gccgaccaag ctgcggccgg gctcaagaat gagtttcaga cggcgtgtgc cgatcagttt
     2341 ttgaacggct tgggcatacg cgcccaaatc aggcacattt tcgtcttggt aaacaatgcc
     2401 gacgccgccg cccaagtcca agtgttccaa cacaatgcct tcggcggcaa gcgcgtcaac
     2461 caaaatcaaa atgcgctcgc aggcttcgac cagcgggctt aagtcggtca gttgcgaacc
     2521 gatgtggcag tcgatgccga tgattttcaa attgggctgt tgtgcggcat ggcggtaggc
     2581 ttcgagcgcg tcggcgtagg caatgccgaa tttgttggct ttcagacctg tggagatgta
     2641 gggatgggtt ttcgcatcga catcgggatt gacgcgtagg gagacggacg cggttttgcc
     2701 caaacgcgcg gcaacctttt gaatacggtc gatttcggga atgctttcca tattgaagca
     2761 tttcacgcct gcattcagcg cgaactcgat ttccgcctcg tttttgccca cgccggaaaa
     2821 aatcgttttc gtcgcgtcgc cgcctgccgc caaaacgcgt gccaattcgc cgccggacac
     2881 aatgtcaaaa ccgctcccca aagaagcaaa gtgtttgatg atgctcaggt tgccattggc
     2941 tttaaccgca tagcagacga gcggggagag ctcggcaaag gcagtttggt agtgttcaaa
     3001 tgcttcggtc agcgcggatt ggctgtacac ataaagcggt gtgccgaatg cttcggcaag
     3061 gcgggggtag gggacttgtt cgcaaaacag ggtcatatct tcgttttcat tttcgggttt
     3121 gtggggcgga ttgcggtttg ttttgaagct gcaaaccggt ttggattgcg ccgaaacgcg
     3181 ccgtatcgcc ttctttgggc aggtagaggt cgcctttgta accgcaggcg gaaagcagga
     3241 gggcggttgc cgccgcaaaa aatacgccgt atttcatcgg taaacttcct tcataagcgc
     3301 gaatgtggca agattcggta tcttaaacaa aaaccacaca aaaagccatg atgaccgaaa
     3361 gcgagtttat ccgcgcgagc gaagcattat ttgaacacat cgaagaccaa atcgacgaaa
     3421 acggctggga tttcgactgc cggtttgccg gaaacgtcct gaccatcgaa gccgaaaacg
     3481 gcacgcaaat catcgtcaac cgccacacgc ccaaccaaga attatggatt gccgccaaaa
     3541 gcggtggcta ccatttcgcc gaacaaaacg gcaaatggct ggcaacgcgc gacggacggg
     3601 atttttacga cgttttaaac gaagccctga gcgcggcttc gggcgaagcg gtagagattg
     3661 aggaattgtg atttgggtgt tatcacggaa agaaaaaatg aacacacgtc ccttttattt
     3721 cggacttata tttatcgcga ttattgctat acttgctaac tatttaggaa acactgattt
     3781 ttcccatcat tatcatatca gtgctttaat tattgctatc ttgctgggaa tggcaatcgg
     3841 caataccatt tatccgcaat tttcaacaca agtggaaaaa ggtgttttgt ttgccaaagg
     3901 cgcgcttctt cgcactggca ttgtgttgta tggttttcgc ctcacttttg gcgatattgc
     3961 cgatgtagga ttaaatgcgg ttgtcactga tgcaatcatg ctaatttcaa ccttcttttt
     4021 taccgcactt ttaggcattc gttatctaaa aatggataaa caattggttt atctcactgg
     4081 ggcaggttgc agcatttgcg gtgcggcagc ggttatggcg gcagagtctg ttaccaaagc
     4141 agaatcccat aaagtttcag tggcgattgc catagtggtc attttcggga cgcttgctat
     4201 ttttacttac cccttgttct acacgtggtc acaagattta attaacgccc atcaattcgg
     4261 tatttatgtt ggttctagtg tacacgaagt ggctcaagtg tatgcgattg gggaaaatat
     4321 taatcctatc gtggcgaata ctgccgtcat ttccaaaatg atccgagtga tgatgctcgc
     4381 ccccttttta ttaatgcttt cttggttatt aacacgtagt gatggggtat cagaaaatac
     4441 ctcacacaaa attacaattc cttggtttgc tgtacttttt attggtgttg ccatttttaa
     4501 ttcttttgat ttattaccaa aagaactcgt gaaattattc gttgaaatcg attctttctt
     4561 attaatttca tcaatggctg cgcttggctt aacgacgcaa gcaagcgcaa tcaaaaaggc
     4621 aggattaaaa ccgtttgttt taggaatact aacttattta tggctagtgg ttggtggatt
     4681 tttagtgaac tatggaatat caaaattaat ataaaattca ctaaagagag cgttacccaa
     4741 tggcacaatt acccctgtat cggactgccg aaatcgaaaa ttttactgtc ggcacgcccg
     4801 aagttttaga atcattttcc aaacatatcc cttatggtgt tgtctttgaa gacgacggcg
     4861 acacaggcta cttctatgcc gcttcgcaag acgggatttt agatgccttg cacatttaca
     4921 atgtcgaaga tgtatccgat aaacatatcc ccaatcatgt cttgatttta tgggatgatg
     4981 cctgcaccat agccgcattg tgtatcaacg actacattca tgccgtctat gattttgtcg
     5041 aacaggcagg atattgccgc aacggcttcc ctgaagcaca aggcgaatgg ccgaaagtca
     5101 cagaccgcgt attggacgat gaattgcagg acaaaatcct atcccgaaaa cctacataac
     5161 ctactcaaaa ggataaccca atgcccctac tagacagttt caaagtcgat cacacccgta
     5221 tgcacgcccc cgccgtacgc gtggcgaaaa ccatgaccac gcccaaaggc gacaccatta
     5281 ccgtattcga cctgcgtttc tgcgtgccca acaaagaaat cctgcccgaa aaaggcatcc
     5341 acacgctgga gcatttgttc gccggtttta tgcgcgacca cttgaacggc aacggcgtgg
     5401 aaattatcga catttccccg atgggctgcc gcaccggttt ctacatgagc cttatcggca
     5461 cgccttccga acagcaggtc gccgatgcat ggctcgcctc gatgcaggat gtgggcaatg
     5521 tcaaagacca aagcaaaatc cccgagttga acgaatacca atgcggcact tatcaaatgc
     5581 actcgctcgc cgaagcgcag caaatcgcgc aaaacgtgtt ggcgcacaaa gtggcggtga
     5641 acaaaaacga agagctgacg ctggatgaag ggctgctgaa cgcctaatcc gccaaaaatg
     5701 ccgtctgaac aagggtttca gacggcattt gccttttccg ttataatccg gggttgtgcg
     5761 gggtcgggtt ttaagccggc atcgtccttc cctatttttt ctgtccctta tcggttttaa
     5821 gcgggttttt tatgtccaac agacctacac tcctcctcgt tgacggctcg tcctacctct
     5881 accgcgcgta tcacgcgatg gcgcagttga ccgcgcccga tggtgcgccg acgggcgcga
     5941 tgtatggcgt gttgaacatg ctgcgccggt tgcgggcgga ttatgtgcat gattattgcg
     6001 cggtggtgtt tgatgcgaag ggcaagaatt tccgccacga gatgttcccc gactacaagg
     6061 cgacgcgccc gccaatgccg gacgatttgc gtcctcaagc ggaagccttg ccggatttgg
     6121 tgcggttgat aggctggccg gtgctggtga ttccgcaagt cgaagcggat gacgtgattg
     6181 gcacgctggc ggcaatggca ggcgaagcag gttggaatgt ggtggtgtcc accggcgata
     6241 aggacatggc gcagttggtg aacgaacgcg taacgctggt gaacacgatg agcggcgaaa
     6301 cgctggacat tgaaggtgtg aaggcgaaat tcggcgtgcg ccccgaccaa atccgcgatt
     6361 atctcgcgct gatgggcgac aaggtggaca acgtgccggg cgtggaaaag tgcggcccga
     6421 aaacggcagt gaaatggctg gaagcctacg gctcgctggc gggcgtgatg gaacacgcgg
     6481 cggaaatcaa gggcaaagtg ggcgagaatc tgcgtgccgc cctgcagcgc ctgccgctgt
     6541 cgtatgattt ggtgacgatt aaaaccgatg tggacttgca caccgaactt tcagacggca
     6601 tcgaaagcct gcgccgtacc gcgccgaaat gggcgcaact ggttgtcgat ttcaaacgct
     6661 ggggcttccg cacctggctg aaagaagcgg aaagtcgtat gcacgaagcg gcggacggtg
     6721 atttgttcgg cagcgacagc atcggcgagc aggcggcttt gaatgcggag gtgccgtctg
     6781 aaaaacaacc cgaacttgcc ccgacccccg aaaaactgga ttatcaagcc gttaccaccg
     6841 aagcgcagct tgccgccttg ttggacaaac tgtcgcaggc ggacacaatc ggcatcgata
     6901 cggaaaccac gtcattagac gcgatgaacg cctcgttagt cggcatcagc atcgcgttcc
     6961 aagcaggcga agcgatttac atccccgtag ggcacagcct gaccgctgca cccgaacaga
     7021 ttgatttgca agacgtatta ggccgtctga aaccgcattt ggaaaacccc gccctgaaaa
     7081 aaatcgggca aaacctcaaa tacgaccaac acgttttcgc caactacggc attgccttaa
     7141 acggcattgc cggcgacgcg atgcttgctt cctacatcat cgaaagccac ctcggacacg
     7201 gcttggacga attgtccgaa cgctggctgg gcttggaaac cattacctac gaatcgctgt
     7261 gcggcaaagg cgcgaagcaa atcagttttg ccgatgtcgc catcggacag gcgaccgaat
     7321 acgccgccca agacgccgat ttcgccctgc gcctcgaagc gcatctgcgt gcgcaaatgg
     7381 acgccaaaca gcttgaaatg tatgaaaaaa tggagctgcc cgtcgcgcag gtattgtttg
     7441 aaatggaacg caacggcgtg caaattgatc gcgccgaact ctcccgccaa agcgcggaac
     7501 tcggcgcaga gctgatgaag ctcgaacaag aagcctatgc cgtcgcaggc cagccgttta
     7561 acctcaactc gcccaaacag ctccaagaaa tcctgttcga caaaatgggc attcccacca
     7621 aaggcctgaa aaaaaccgcc aaaggcggca tttccaccaa cgaagccgtg ctcgaacagc
     7681 tcgcacccga ctaccccctg cccaaaatca tcctgcaaaa ccgcagcctg gcgaagctca
     7741 aatccaccta caccgacaaa ctgcccgaaa tgatttcccc caaagacggc cgcgtgcata
     7801 ccacctacgc ccaagccgtc gccattaccg gccgtctcgc cagcaacaac cccaacctgc
     7861 aaaacatccc catccgcacc gccgaaggcc gccgcgtgcg ccgcgccttt accgcaccgc
     7921 aaggcagcgt catcgtttcc gccgactatt cccaaatcga gctgcgcatc atggcgcacc
     7981 tctccggcga caaaaccctg attaccgcgt tccaaaacgg cgaagacgta caccgccgca
     8041 ccgccgccga agtgttcggc attgcccccg aaaacgtctc gtccgaacaa cgccgctacg
     8101 ccaaaaccat caacttcggt ctcatttacg gcatgggcca atacggcctc gccaaatcat
     8161 tgggtatcga caacatctcc gccaaaacct tcatcgaccg ctacttcgcc cgctatcccg
     8221 gcgtcgccga atacatgcag cgcaccaaag aacaagcagc agcgcaaggc ttcgtcgaaa
     8281 ctctgttcgg ccgccgcctc tacctgcccg acatccgcaa caaaaacgcc aacgcccgcg
     8341 ccggagccga acgcgccgcc atcaacgccc ccatgcaagg caccgcctcc gacctcatca
     8401 aacgcgccat gatagacgtg cgaaactgtc tttcagacgg catcggcagc aaactggtca
     8461 tgcaggtgca tgacgaactg gtgctggaag tcgttgaaac cgaactggat tttgtcaaag
     8521 aaaaactgcc gcagattatg gcgaaagtgg acggcggatt attggatgta ccgctggtgg
     8581 ctgaggttgg cgtaggggag aattgggaag aggcacattg attgaaaggt gttatatgct
     8641 atctttattt aaataaaatt taatttttgg tatatttttt ctaaatgttc cta
//
LOCUS       DAXHPG010000081         8562 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXHPG010000081 DAXHPG010000000
VERSION     DAXHPG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8562
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.081"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(77..595)
                     /locus_tag="ACFM6F_001338"
     CDS             complement(77..595)
                     /locus_tag="ACFM6F_001338"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC6164740.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTKQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(615..2000)
                     /locus_tag="ACFM6F_001339"
     CDS             complement(615..2000)
                     /locus_tag="ACFM6F_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC6164741.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KTIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            2239..3780
                     /locus_tag="ACFM6F_001340"
     CDS             2239..3780
                     /locus_tag="ACFM6F_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6164742.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAEVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGK
                     QTETL"
     gene            3809..4408
                     /locus_tag="ACFM6F_001341"
     CDS             3809..4408
                     /locus_tag="ACFM6F_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC6164743.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            4405..5010
                     /gene="nadD"
                     /locus_tag="ACFM6F_001342"
     CDS             4405..5010
                     /gene="nadD"
                     /locus_tag="ACFM6F_001342"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC6164744.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRRGETYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            5069..5455
                     /gene="rsfS"
                     /locus_tag="ACFM6F_001343"
     CDS             5069..5455
                     /gene="rsfS"
                     /locus_tag="ACFM6F_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC6164745.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            5509..5979
                     /gene="rlmH"
                     /locus_tag="ACFM6F_001344"
     CDS             5509..5979
                     /gene="rlmH"
                     /locus_tag="ACFM6F_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC6164746.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAVPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            6052..6318
                     /locus_tag="ACFM6F_001345"
     CDS             6052..6318
                     /locus_tag="ACFM6F_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC6164747.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            6475..7158
                     /locus_tag="ACFM6F_001346"
     CDS             6475..7158
                     /locus_tag="ACFM6F_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFC6164748.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            7282..7794
                     /gene="coaD"
                     /locus_tag="ACFM6F_001347"
     CDS             7282..7794
                     /gene="coaD"
                     /locus_tag="ACFM6F_001347"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC6164749.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            7826..8479
                     /locus_tag="ACFM6F_001348"
     CDS             7826..8479
                     /locus_tag="ACFM6F_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFC6164750.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1851 a         2274 c         2508 g         1929 t
ORIGIN      
        1 aatgtaatcc attctgttcc cgatgtttat gccgtctgaa acccatcctg tgtcgggctt
       61 cagacggcat attgcttcaa agcaggtttt ccgaggcaac ccagttcaga atatcggctt
      121 cgaccgcttc gggcgtgccg gggttggcga ttttgacccc gtattcgcct tcgcccagtt
      181 cctcgaggat ttccagttgc gaatcgagca tccctgcttt catataatgt cctttgcgcg
      241 acatcatgcg ctcgaggttg atgtcttgcg gcggactgag gtggatgaag gcagctttgc
      301 cttcggctcc gcgcagaatg tcgcggtagc cgcgtttgag ggcggaacag gttacgatgg
      361 tgtggttcgc accgttttgc gcctgtttcg tcatccagtc gcgcagattg cccaaccacg
      421 gatagcggtc ttcatcggtc agcggaatac ccgcgcccat cttgtcgcgg ttggcttggg
      481 tgtggaactc gtcgccttcg gcatagggac attgaccgag gtgtttctgc agggacagcg
      541 cggcggtggt cttgccgcag ccgcatacgc ccataacgac aaaatgcgta gtcatttact
      601 atcctttccg tctgtcagac gatggcgaac agcagtgcgg acaaggcaaa gccgatgagt
      661 gcgatgaggg tttggttgac cgtccaggtt ttcagcgtgg tcggtacgtc catgtccaag
      721 aggcggccga ccagccagaa gccggagtcg ttgaagtggc tgcaaccgac cgaacctgcc
      781 gccgttgcca atacgataca ggcgagctgc cagtcggtaa agccggcggc ggcaacggca
      841 ggagccatca gcgcggcggc ggtggtcagg gcgacggttg ccgaaccttg cgcgatacgc
      901 agtgccaagg cgacaaggaa acagcccaaa aggacgggaa tgcccaaatc cgccatgctg
      961 tcggcgagtg ccttgccgat gccggaagcg cgcaaaacgc cgccgaacat accgcccgcg
     1021 ccggtaatca gaatcacgga acagacgggg gcgagtgcgc cgtccacggt tttttccaac
     1081 gcgctgccgc tttcgccgcg tttgcgtccc aagacaaaca gtgcgaccaa tacggaaatc
     1141 agaagggcga tcggtgtcga accgattgtt tttgccgtct gaacccaggt ttcgtccgca
     1201 cttacgagtt tttcgctgat gagggccgat acgccggtat tcaggaaaat cagcagcatg
     1261 ggaatcagca tgatggcgac gaccgttcct gctttggcag gttctttcgg caggtcgttg
     1321 tcttgcgtgc cgccgctgag cagttcggga acgggaacat ggatggtgcg ccccaacact
     1381 ttgccgagca tatagccgct gaaataccat gtgatgaagg cggtcggcag acccaaaatc
     1441 aaaacttggc cgatgttcgc gccgtaaaat tcggaagcgg caatcgggcc cggatggggc
     1501 ggcaggaaga cgtgcatgac ggaaaatgcg ccgatggagg caagcgcgaa gggcagtacg
     1561 tcctgtttca tgcgccgtgc ggtggcgaac acgatgggca gcatgacgat tagtccggca
     1621 tcgaagaaaa tcgggaagcc gaaaatcagc gaggcaacgc ccagcgcgaa cggtgcgcgt
     1681 ttttcgccga acatccggat cagcgcgtcc gccagcgact gtgcgccgcc ggatgtttcg
     1741 accaaacgtc cgagcatcgc gcccaggccg accagaagcg ccacgccgcc gagcgtgccg
     1801 ccgaagtttt tgaccagtac gtcgttgaca atgctgcctg tgggcaaacc ggttgccaaa
     1861 gccgtcagca ggctgacgat gaccagtgtc agcagcgcgt ggatgcggaa tttgacgatt
     1921 aaaatcagaa tgaggatgat tgccgccgcc gaaatgccca acagggtttg cgcggacagc
     1981 gtctgtgtcc agccgtccat ttgtaaagcc tttctctgta ataaaagtac aaaattgtca
     2041 ggttttttta agttgtgcga aaacgatagc acaaatcggg cggtaagttg tttgtctgaa
     2101 gttatattcc tgtttatttg aacgatttat ttcagacggc gggaggaagg cggatacggt
     2161 atgtagtcgg attgaaatat acggtatgtg gggcttttgg gtacaatcgc aacatcagtg
     2221 tttcaagtgg ggaaggcgat ggatggacgg cgttgggcgg tatggggtgc ttttgccctg
     2281 ctgccttcgg cttttttggc ggtaatggtc gttgcgcctt tgtgggcggt ggcggcgtat
     2341 gacggtttgg cgtggcgcgc ggtgctgtcg gatgcctata tgctcaaacg tttggcgtgg
     2401 acggtatttc aggcagcggc aacctgtgtg ctggtgctgc ctttgggcgt gcctgtcgcg
     2461 tgggtgctgg cgcggctggc gtttccgggg cgggctttgg tgctgcgcct gctgatgctg
     2521 ccttttgtga tgcccacgtt ggtggcgggc gtgggcgtgc tggctctgtt tggggcggac
     2581 ggcctgttgt ggcgcggacg gcaggatacg ccgtatctgt tgttgtacgg caatgtgttt
     2641 ttcaaccttc ctgtgttggt cagggcggcg tatcaggggt ttgtgcaagt gcctgcggca
     2701 cggcttcaga cggcacggac gttgggcgcg ggggcgtggc ggcggttttg ggacattgaa
     2761 atgcccgttt tgcgcccgtg gcttgccggc ggcgtgtgcc ttgtctttct gtattgtttt
     2821 tccgggttcg ggctggcgct gctgctgggc ggcagccgtt atgccacggt cgaagtggaa
     2881 atttaccagt tggtcatgtt cgaactcgat atggcggttg cttcggtgct ggtgtggctg
     2941 gtgttggggg taacggcggc ggcagggttg ctgtatgcgt ggttcggcag gcgcgcggtt
     3001 tcggataagg cggtttcccc tgtgatgccg tcgccgccgc agtcggtcgg ggaatatgtg
     3061 ctgctggcgt ttgcggcgga ggtgttgtct gtgtgctgcc tgtttccttt gttggcaatt
     3121 gttgtgaaag cgtggtcggc cggcgaatcg tggcgtgtgt taatggaaag tgaaacgtgg
     3181 caggcggtgt ggaatacttt gcgcttctcg gcggcggcgg tgtttgcggc ggcggttttg
     3241 ggtgtggtgt atgcggcggc ggcgcggcgg tcggcgtgga tgcgcgggct gatgtttttg
     3301 ccgtttatgg tgtcgccggt ttgtgtttcg gcgggcgtgc tgctgcttta tccgcagtgg
     3361 acggcttcgt tgccgttgct gctggcgatg tatgcgctgc tggcgtatcc gtttgtggca
     3421 aaagatgttt tgtcggcttg ggacgcactg ccgccggatt acggcagggc ggcggcgggt
     3481 ttgggcgcaa acggctttca gacggcatgc cgcatcacgt tccccctctt gaaaccggcg
     3541 ttgcggcgcg gtctgacttt ggcggcggca acctgcgtgg gcgaatttgc ggcaaccttg
     3601 ttcctgtcgc gtcccgagtg gcagacgctg acgactttga tttatgccta tttggggcgt
     3661 gcgggtgagg ataattatgc gcgggcaatg gtgttgacat tgctgttgtc ggcgttcgcg
     3721 ctgggcattt tcctgctgtt ggacaacggc gaaggcggaa aacagacgga aacgttataa
     3781 tgtgaaccct ttttcagagg acggcaaaat gagcgagttg gataacatcc ttgcccataa
     3841 ccggcagttt gtcgagtcgg gcgaatatga aaaatacttt accgacaaat accccgaacg
     3901 cgggctggca gttttgtcct gtatggatgc gcggattatc gggctgctgc ccgacgcgtt
     3961 gggtttgaaa aacggcgatg ccaagctgat taaaaatgcc ggcgcgctgg ttacgcaccc
     4021 gtggggttcg gtgatgcgga gtcttttggt tgccgtgttt gaattgaagg tcagagagat
     4081 tatggtcatc gcccatcacg attgcggtat gcaggggctg aatgccgaag aattcctcgg
     4141 gcgcgtccgg gaaagccaga ttcccgaaga ccgtatcgaa accctgcgtt atgccggtat
     4201 cgacctcgac ggctggctga ccggtttcga caacgtcgaa gacagtgtgc gccacacggt
     4261 ggaccttatc cgtaaccatc cgctgatgcc gcgccatatc gccgttcacg gactggtcat
     4321 ccatcccgtt accggcaaac tgacgctggt tgtggacggc agtgtttcag acggcatgga
     4381 cttatcggaa ggaatggaaa catcatgaag aaaatcggac tgttcggcgg cactttcgac
     4441 ccgatacaca acgggcatct ccacatcgcc cgcgcctttg ccgacgaaat cggcttggac
     4501 gcggttgttt tcctgccgac aggcggcccg tatcacaaag acgccgcctc cgcttccgcc
     4561 gccgaccgcc ttgccatggt cgaattggcg acggcagaag atgcgcgttt tgccgtcagc
     4621 gactgcgaca tcgtccgccg gggcgagact tacactttcg acaccgtcca aattttccgc
     4681 cagcagttcc cctcggcgca actctggtgg ctgatgggca gcgacagcct gatgaagctg
     4741 cacacatgga aaaaatggca gatgctcgtg cgcgaaacca atatcgccgt cgccatgcgg
     4801 cagggcgaca gcctgcacca aaccccgcgc gaactgcacg cgtggctggg caatgcgctt
     4861 caggacggca gcgtccgcat cttgtccgcc ccgatgcata atgtgtcgtc aacggaaatc
     4921 cgccgcaacc ttgccggcca aggcgtttca gacggcatcc cgcctgccgc cgcacgctac
     4981 atccgcgaac acggtttgta tgaaaaataa agtcaaatca gtaagaaacg gctataatgc
     5041 cgtctgaaaa catcccgtta ggaacataat gaacgaacaa gaactgcaag acctgcaaaa
     5101 aatggtcggg gtcgccgtca acgccctcga agacatcaaa gccaaagaca tttccgttct
     5161 cgaaacgcaa gataaaactt cgctgtttgc cagaatgatt atcgccagcg gcgacagtac
     5221 gcgccaagtc aaagcactgg ccaacaacgt tgccgtcgat ttgaaagaag ccggttttga
     5281 aatcctcagt accgaaggcg acagcggcga atggacgttg gttgatgcag gagacctcgt
     5341 tgtccacgtt atgctccctg ccgtgcgcga cttctacgac atcgacaccc tttggggcgg
     5401 cgagaaaccg agtttccacg ccggaatgca gaagccgtgg cacgcggcag actgattccc
     5461 gatgccgtct gaatgttcag acggcatttt cctgtaaggg agaaagcatt gaacattacc
     5521 gttttggcag tcggcaccaa aatgccgcgc tgggttgatg aggccgtcgc cgaatacgcc
     5581 aaacgcttcg gacgcgacgt cgcctacgca ctcaaagaaa tcaaacccga aaaacgcggc
     5641 gcgggcgtga atgccgccca aggtatggcg gcggaagaaa aacgcatcct tgaagccgtt
     5701 ccgcaaggcg cgttccttgt cgttcttgac gaacgcggca aagcgccgac ctccgtcgag
     5761 ctggcggaac acctcaaaag ctggcggcaa aacggcgaac acgtctgctt cgtcatcggc
     5821 ggcgcggacg gcatgaccga ccgcctcaaa caacaagccc gcatgatgat gcgcctgtcc
     5881 agcctcaccc tgccgcacgg catggtgcgc gtccttctga ccgagcagct ctaccgggcc
     5941 gtttccatcc tgcacaacca tccttatcat cgggaataag aaggcttttg tccgcccccc
     6001 taatcggtta aaatcacccc gttatttctg aacacagcaa aggaatccgc tatggcacgc
     6061 atggtattct gcgtcaagct caacaaagaa gccgaaggca tgaaatttcc gccgcttccc
     6121 aacgaattgg gcaagcgcat ttttgaaaac gtatcgcaag aagcgtgggc ggcgtggacg
     6181 cgccaccaaa ccatgctgat taacgaaaac cgcttaagcc ttgccgatcc gcgcgcgcgc
     6241 gaatacctgg ctcagcagat ggagcagtat ttcttcggcg acggcgcgga tgccgttcag
     6301 ggatacgttc cgcaataacg gttttccgtt ttgaacacag gctgtccgaa actgcttcag
     6361 acggccttta aaatacgctg gacaacttta tcttcacgca accggacaaa ttttgacatt
     6421 gggcaatgtt aaaatccccc attattccca accgcgcttt caggagcaga tgatatgcaa
     6481 cacgacgttt acgactacac cgcgcatacg gtttctaaaa acaccgtcct gcagaaaacc
     6541 taccgcctgc tcggattttc attcattccg gcagccgcag gcgcggcact tgccgccaat
     6601 gccggtttca atttttacgc cgccttcggt tcgcgctgga tagggtttgc cgtcgtgttg
     6661 gcgtttttct acggtatgat ccacttcatc gagaaaaacc gttacagcaa taccggcgtt
     6721 accctgctga tggtattcac attcggtatg ggcgtattga tcggccccgt gctgcaatac
     6781 gcgctccata ttgccgacgg tgcgaaaatc gtcggcattg ccgccgcgat gaccgccgcc
     6841 gtctttttaa cgatgtccgc cttggcgcgc cgaacccggc tcgatatgaa cgcgctcgga
     6901 cgcttcctga ccgtaggtgc ggtcattctg atggtcgccg tggttgccaa tctgtttttg
     6961 ggtattcccg cactcgccct gaccatttcc gccggttttg tcttgttcag ttccttaatg
     7021 attatgtggc aggtacgcac cgtcatcgac ggcggcgaag acagccacat cagcgcggca
     7081 ctgacactgt ttatctcgct ttacaacatc ttcagcagcc tgctgaacat cctgttgtct
     7141 ttaaacggcg aggattgatg ccggcaaaac gccgtccgac tatgccgtct gaaaatgctt
     7201 cagacggcat tttactttgg gcatacaata cggatgcaca cgcaccaaca cacagccgac
     7261 tatgaagccg ggaggaatca aatgttaccg aatacaccgc gccgcgccgt ttatgccggc
     7321 agtttcgatc cgcccacatt ggggcatctg tggatgatac ggcaggcgca atctatgttt
     7381 gacgaactca tcgtcgccat cggcattaac cccgacaaac gcagcaccta taccgtcgct
     7441 gaaaggcagg atatgttgtg cgatattact aaaatgtttc ccaacgtcag aaccgatgta
     7501 tttgaaaacc gatttctggt gcattacgcc cgtgaggtag atgcaggatt catcgtgcgc
     7561 ggcatccgtt ctgcttcgga ttacgaatac gaacgttcca tgcgccatat caacagcgac
     7621 ctcgcccccg aaatatccac cgtattcctc atgccgccgc gcgaaatcgc cgaagtgtcg
     7681 tccactatgg tcaaaggact ggtcgggccg gaaggctgga cggaaacgat ccaccgctac
     7741 gtgccgcaag ctgtgtacga aaaaatcctt gccgaacatc aacacgaaaa ttgaaccctt
     7801 ctatttttgc aaggcatata tggatatgtt ggaaattttg ttccgccatc aagacttcgt
     7861 cgccatcaac aaaccgggcg gcatatccgt ccaccaagac agcggcgaga ccggactggc
     7921 ccgaacactc gccatacagt tgggcgtaga gcgtgtgtgg ctgctgcacc gattggacaa
     7981 acagaccagc ggcattttac tgtttgccct caacagagaa agcgcgtccg cccttagcgg
     8041 acagtttgcc ggcaaaagca tcaaaaaaac ctatttggca ctatccgacc gcaaaccgtc
     8101 caaaaagcag ggttggatta aaggcggaat ggaaaaatcc agacgcggaa tgtggaagtt
     8161 gacgcgcaat acagaaaata tcgccgttac ccgattccac agcatcagta tcgccgaaaa
     8221 actgcggctg ttcatcctgg aaccgcatac gggcaaaacg caccaattga gagtggcgat
     8281 gaaaagtttg ggcagtccga ttctgggcga cagcttatac ggcggaacag aatccgaaac
     8341 catgtttctg tatgcatgga aaatacaatt tgcctaccaa aaccgacaaa tcgaaattgt
     8401 tgcaccctta aaaaacgaat ggcagactga aaatatctct catgcgctgg aagaatttta
     8461 tatggcaaaa gcaggttaat aaatataaat taatttataa acaatatgat aaatattttt
     8521 ataaaaattt ttcaagaaaa cggttgactg caaatctctt ca
//
LOCUS       DAXHPG010000082         8513 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXHPG010000082 DAXHPG010000000
VERSION     DAXHPG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.082"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            96..1280
                     /gene="coaBC"
                     /locus_tag="ACFM6F_001349"
     CDS             96..1280
                     /gene="coaBC"
                     /locus_tag="ACFM6F_001349"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC6164751.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIDKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDEELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1347..3503)
                     /locus_tag="ACFM6F_001350"
     CDS             complement(1347..3503)
                     /locus_tag="ACFM6F_001350"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6164752.1"
                     /translation="MPVPQPTAPYDHLTAEARTLLFRTASYLKPEEQVELEKAVAYAF
                     HAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKMVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3596..3802)
                     /gene="rpoZ"
                     /locus_tag="ACFM6F_001351"
     CDS             complement(3596..3802)
                     /gene="rpoZ"
                     /locus_tag="ACFM6F_001351"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC6164753.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3861..4478)
                     /gene="gmk"
                     /locus_tag="ACFM6F_001352"
     CDS             complement(3861..4478)
                     /gene="gmk"
                     /locus_tag="ACFM6F_001352"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC6164754.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSQEVIQRRLAKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4606..5205
                     /locus_tag="ACFM6F_001353"
     CDS             4606..5205
                     /locus_tag="ACFM6F_001353"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC6164755.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5251..6039)
                     /locus_tag="ACFM6F_001354"
     CDS             complement(5251..6039)
                     /locus_tag="ACFM6F_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC6164756.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAVKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            6364..7719
                     /gene="yegQ"
                     /locus_tag="ACFM6F_001355"
     CDS             6364..7719
                     /gene="yegQ"
                     /locus_tag="ACFM6F_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC6164757.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDILEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHHTQDYQNYLTGHSTAKQSQYVGHVTEIDENGWATVEVKNR
                     FAVGDSLEIIHPNGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7853..8329
                     /locus_tag="ACFM6F_001356"
     CDS             7853..8329
                     /locus_tag="ACFM6F_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC6164758.1"
                     /translation="MLAVCQSGSAPKNAHTASAGTPAGESCKVIRDDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRGVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
BASE COUNT         2159 a         2318 c         2019 g         2017 t
ORIGIN      
        1 ttcagacggc attttgtatc caaaacggta actaatgtat ccgtactttg ttatagaatg
       61 gctgctgttt ttcttcgtaa ttagaaattg tcaaaatggg caaacatatt cttttaggtg
      121 taacgggcag tattgcggcg tataagtctt gcgagttggt gcgactgctg aaaaaacagg
      181 ggcattcggt tacggtggtt atgagccgct cggcaactga atttgtttct ccgctgactt
      241 ttcaggcttt aagcggcaat cctgtcctga ccgacacgca cggcggcaac ggttcaaacg
      301 gtatggaaca tatcaacctg acccggaatg cggatgtttt tctgattgcg ccggcaagta
      361 tgaataccgt ggcaaaaatc tgtaacggcg tggcagataa cctactgacc agtctggcag
      421 ccgcacggaa atgtccgctt gccatcgctc ccgcgatgaa tgtggaaatg tggctcaacc
      481 ctgccaacca acggaatatc gcacaactgg tttcagacgg cattactgtc tatatgccgg
      541 gcttgggcga acaggcttgc ggagaaaatg gcatgggaag gatgccggaa cctgccgaat
      601 tgctggattt gcttccggac ttgtggacac cgaaaatttt aaggggcaag aaaatcttga
      661 ttaccgcagg tgcgacattt gaagccattg accctgtcag gggcatcaca aatacctcca
      721 gcggaaaaat gggggtagct ttggcgcggg cgtgccgtgc cgccggtgcg gaagtcagcc
      781 tgattcacgg acagcttcaa accgcgctgc ctttcggcat atccgatacg gttcaagccg
      841 tcagcgctga agatatgcat cgcgcagtgc atcgtttaat cgacaaacaa gatgctttta
      901 tttctgttgc cgccgtctca gactataagg ttaaaaacag gagtacgcaa aaattcaaaa
      961 aagataaaaa tgccaaaccg ttatccatcg aattggatga gaaccccgat attttggctt
     1021 ctattgcctc attgccgaac ccgccgttct gcatcggttt tgccgctgaa acggagaatg
     1081 taatggcata tgcgcgagaa aaacgtatta agaaaaaaat accagtgatc gttgccaatg
     1141 atgtttcaat cgcgatgggc aaaccgacca accggattac cattatcggg gacgacgagg
     1201 aactgtcttt tcccgaaaca agtaaagatg aagcggcaat gcagattgtt gaaaggcttg
     1261 ccgtatattt gaacaaataa gcaattgaac ggataaacca taaagcgggt tgcctgttaa
     1321 tcaaaaggca acccgtttta cctgcttcaa cttctgatga ctttgcggat atatggaata
     1381 ctatgcagat tttgaataat ctgattcaat tgattcagat tcttgacttt caataagaat
     1441 ttgaattcga caaaaccttc cgttcccgac tgggatttag acggtgtttc gaccgactca
     1501 atgtccgcac cggaatcgga aatcgcttgc gccattaatg ccagcaagcc gtggctgtct
     1561 tccgattgga cttgaagccc gacacggtag ttctgcccgt tcatattttc ccagtctgca
     1621 tccagctgct gttcgggatc ggactttaac aacgtcgggc aggtatccct atggataatc
     1681 atgccttttc ccttaaccaa cagcaaacgg atggaatcgc cgggaaccgg atggcagcac
     1741 tctgcaaaat gaatatgccc gctttcctga ccatcaactt taatggaact gagcctgacc
     1801 tcgctgccga aatgctcccc tgccaactcg gcaatgtgca tggcgacata aacaggcagg
     1861 gtatgcccca taccgacgtt gtacagcact tcctcaaatg aagtctgttt gtcgttgaga
     1921 tcggcaaggt atttttcttt aatgccgtct gaaagcagga catctttggg cagcaaactg
     1981 gacagggctt tttgtaagag gctctctccc aaaacgaccg catcgtgccg gttaaggttt
     2041 ttaatatatt ggcgtatggc actgcgcgcc ctgcctgaca cggcgaaatt caaccatgca
     2101 ggattgggtt tggcatgttc ggatgtaata atttcgacgg aatcgccggt tttgagcttc
     2161 gtacgcaacg gcatcatgat attgttgata cgtgcggcaa cggttttgtg cccgatatcg
     2221 gtatgcaccg cataagcaaa atcgacaggc gttgcccctt tgggcaaagt taggattttt
     2281 ccttttggcg taaggatgta gatttcgttc ggaaacaaat cgactttgac gtgttcgaga
     2341 aactcaatgg cattggcact gcttgcctgc aaatctaaga tatttttcag ccaccggttt
     2401 gtgtgaagca ccgcctgatc gaccatctta gaatatgatt tatagctcca atgtccggcg
     2461 attccacctt cggcaacagc atccatttcc ttggtacgta tctgaacttc aatcggcaag
     2521 ccgtaagggc cgaccaaagt cgtatgcaga ctttgatacc cgttgctttt cggaatggcg
     2581 atatagtctt tgaaccgccc gggcttgggc tgatagaggg tgtgcaatgc gccgagtgcg
     2641 gcataacagg ctggaatgct gttgacaatg acgcggaaac cgtaaatatc cataacctcg
     2701 gcaaagcgca gctttttcgc catcattttc tgatggatgc cgtacaggtt tttttccctg
     2761 cctttgattt tggcctctat attcgcgcct accagccgct ggccgaatgc gcgcaagact
     2821 ttgccgacaa cgtcctgccg gttcttccgg ctcttgtcca tcgctttttt taaagtctcg
     2881 tagcggttgg gatgcaggtt ttggaacgat aaatcctgaa gctcttgata tgcgttattc
     2941 aaacctatac ggttggcaat ctgtgcatag atttcaaggg tttcccttgc aatccggcgg
     3001 cgtttgtccg ggcgcatcga accgagcgtc cgcatattgt gcaggcggtc ggcaagtttg
     3061 acgacgatga cgcgcacatc tttggtcatt gccaaaatca gtttgcggaa actctccgcc
     3121 tgatgttccg catgatcttc aaatttgagt ttttcgagtt tggacagacc gtccaccatc
     3181 tcggcaatcg tattgccgaa caccgccgcc atttcccctt ttgtcacgcc cgtatcttcc
     3241 aatacgtcgt gcatcacgcc tgcacacaga ccctgtatgt ccatatgcca aagggcgagc
     3301 tgcgtcgcaa cggcaattgg atgcgtaatg tatggttcgc cacttttccg ggtttgtccg
     3361 tcatgagcgt gaaatgcata ggcaacggcc ttctcgagct caacctgttc ttcaggcttg
     3421 agataggaag cggtacggaa aagcaaggtg cgtgcttcgg cagttaaatg gtcgtaaggt
     3481 gcggtgggtt gagggacagg catttcagac ggctttcgga acatatttta tttttcgttt
     3541 caaaccgtcg gactgcacgg cggcagaatg tgccgacgtg cgtttccggc agaatttatt
     3601 tattgcgcgt caacagttcc gtaccgatat gcccggcggc gatttccctt aaggcggtaa
     3661 cggtcggttt gttattgcgg acatcgtcca caagcggcgt attgccattt tcaagctggc
     3721 gggcccgacg ggccgctacc aatgtcaggt caaaatggtt ggaaattttt ccggtacagt
     3781 cttcggtggt aatacgtgcc atattatttg ctttctttca aaaatattta aaataggaaa
     3841 ctgggtattt tcgcggtttt ttaggaattt tccaacaaat ctgcaataaa tcccagttgc
     3901 cgcgaccttt tcagacggca ggcattcaca atatggcgca aatcctcctc cgcccgcgcc
     3961 aagtcgtcat tgaccacgac aaagtcgaac aatacggact gttcgatttc atgccttgcc
     4021 ttcgcaagcc tcctttggat aacttcctga ctgtccgtcc cgcgtccgtt gagacgcgcg
     4081 gcaagcacat cgaaagacgg cggcaggata aagatgccga cagcttcagg cagcgcatct
     4141 cgaacctgcg ccgcgccctg aacgtcgatt tccaaaatca cgtcataacc tgccgccgcc
     4201 aacgcattca cgccctccgc gcttgtgccg taatagttgc cgaatacgtc tgcgtattcc
     4261 aaaaaagcct cctgtgcgat aagcgactca aactcttctt tggaaacaaa gtgataatgt
     4321 acgccgtttg cttcgccttc acgcggcggg cgcgtcgtgt gcgacacgga aacgcgcaaa
     4381 ccgttatggt ttgccaacag ccgcgacacc agcgtggttt tgcccgtgcc ggaagcggcc
     4441 gaaatgataa agatgttgcc ttttcgataa gcggacatat tttttacctg tatattttcc
     4501 agccgattgt atcacaatgg acacccagtt tcctatttgc cgatgcccat attttgccgc
     4561 tattgttttg attgatttgg caagcggcag gctgacggct acaatatggc gttaaaaaca
     4621 tcaaacttgg aacacgcaat gctggttcat cccgaagcta tgagtgtcgg cgcgcttgcc
     4681 gacaaaatcc gcaaaatcga aaactggccg caaaaaggca tcttattcca cgacatcacg
     4741 cccgtcctgc aaagtgcgga atacttccgc cttttagttg atttattggt ttaccgctat
     4801 atggatcaga aaatcgacat cgttgccggt ttggacgcgc gcggcttcat tatcggcgcg
     4861 gcactcgcct accatctcaa cgtcggtttt gtccccatcc gcaaaaaagg caagctgcct
     4921 tttgaaaccg tatcgcaaag ctacgcgctc gaatacgggg aagctgcggt ggaaatccac
     4981 accgatgccg tcaaacccgg ttcgcgcgtg ctgctggtcg atgatttggt tgccacgggc
     5041 ggcacaatgc ttgccgggct ggaactgatc cgcaaactcg gcggagaaat tgtcgaagcc
     5101 gccgccattt tggaatttac cgaccttcaa ggcggcaaga atatccgtgc aagcggcgcg
     5161 cccttattta ccctgcttca aaacgaaggc tgtatgaagg gctgaaaacc gaccctgccg
     5221 tctgaaaccg gcagggttgt tatgatgcgt tcaaatcacg cccaaatctt gcaagcccct
     5281 caacacgccg tcttcatcaa cgccggggca aacatatttc gccgcctctt tcactgcctg
     5341 ttccccgtta cccattgcca caccgaaccc gacttcagac agcatttcca catcgttcaa
     5401 accgtcgccg aacgccatca cgtccgccat ttccaaaccc aatgcttcaa ccacgcttct
     5461 gatgccgtct gttttcgaca ttcccgcagg cagcagatcg accgcttcct cgtgccagcg
     5521 caccgttttc aagccttccc gttccacaat atccgaccag ggcggcattt cgttttcctc
     5581 cgcaaacacc agcatctgat acaccggttt gcttgaaaaa taatccttat cggcaaaaaa
     5641 atcgctggcg atatgctgca aggcgcggca cacgcattcc gacagcgcgg acacggcgat
     5701 cccctctccg ccgacaaacg cataatccat gcccaagcca tccaaatgcg cgcaaaccct
     5761 gcccatcaaa ccggcatcca tcggtacttc gcgcacagtt tttccgtgca gcagcgcaaa
     5821 ctgtccgttt atcgttacca cggcatccat gcccgtttcc gccatcatgt ccctgacctt
     5881 ttcgggaatc gtcgccaaag accgccccgt tgccaacgcc gtcaatatgc ctttgccgcg
     5941 caaagccgcc accgccgttt tcacggaagg acgcaaggta tccgtatatt tgcggtacag
     6001 cgtatcgtca atatcgaaaa acacgatttt aggattcatc acattctctc tcgcattcaa
     6061 actgccgcat tgtatcccaa tcaggcaaat acttgataaa tccttataaa tttcccggca
     6121 aaattgaccg cgaacacgaa aaggcggata atccgcccat cctcaaaccc ttttcagacg
     6181 gcatttgcag caatgccgtc tgaaacattt ttacaaagca cacaaatcat gtttcaacac
     6241 acaggacgac acataaagcg tcgccctata tgttgccctg attcgaaagg ggttacaccc
     6301 ctcccaaata aagtctgatc ctgccgccct aaagggcggg gtttcaaccg aaaaggaaac
     6361 acgatgaaag cacccgaact cttattgccc gccggcggat tggaaagaat gcgcgccgcc
     6421 tacgattacg gcgcagatgc cgtttatgcc ggcagcccgc gttactccct gcgcgcccgc
     6481 aacaacgaat ttgccaaact cgacatcctc gagcaaggca ttaaagaagc gcacgaacgc
     6541 aacaaaaaat tcttccttac cgtcaatacg ctgccgcaca attccaaact caaaaccttt
     6601 gtttccgata tggagccctt gattgcgatg aagcccgacg cgctgattat ggcggatccg
     6661 ggtttgatta tgaccgtgcg cgaaaaatgg ccggaaatgc cgatccactt gtccgtacag
     6721 gcaaacacca ccaactactg gggcgtaaaa ttctggcaaa acatcggcgt cgaacgcatt
     6781 attctgtcac gcgaattgag tatggaagaa atcgccgaaa tccgccaaca atgccccgac
     6841 atcgaactcg aagtcttcat ccacggcgca ttgtgcatcg cctactccgg ccgctgcctg
     6901 ttgtcgggct atttcaacca ccgcgatccc aaccaaggca cctgcaccaa ctcctgccgt
     6961 tgggactaca aagtccacaa cgctacggaa agcgatgcgg gcgatgccca gcttctgcaa
     7021 ggtttcaact ttgaaaaagc ccaagaagaa gccaaccaaa actttgaagg catcaacggt
     7081 caaaaacgcc atccctacgc cgacaaggtt ttcctgattg aagaatccaa ccgtccgggc
     7141 gagatgatgc cgattatgga agacgaacac ggcacctaca tcatgaactc caaagacctt
     7201 cgtggcattg aagtggttga gaaactcgcc aaaatcggcg tggacagcct caaagtcgaa
     7261 ggccgcacca agtcgctcta ttatgtcgca cgcgtcgccc aatcctaccg caaagcgatt
     7321 gacgatgccg tcgcaggccg tccgtttgat tacagcctgt tgagcgaact cgaaggcctt
     7381 gccaaccgcg gctacaccag cggcttcctc gaacgccacc acactcagga ttatcaaaac
     7441 tacctgaccg gccattccac cgccaaacaa agccaatacg tcggacacgt taccgaaatc
     7501 gatgaaaacg gctgggcaac agtggaagtc aaaaaccgct ttgccgtcgg cgattcgctc
     7561 gaaatcatcc acccgaacgg caaccaaacc atcaaattgg aacaaatgac ccgcaaaggc
     7621 caacctgtcg atgtcgcacc cggcaacggc attcaggtca aaatccccga tatgcagggc
     7681 aaagaaaaag ccctcatcgc acgcgtattg aacccctgag ccacaatgcc gtctgaaccc
     7741 cgttttcaga cggcattttg ttgaatctta cggtacaatg gcaatgattt cccaccgcaa
     7801 gcaaggatat ccgacatgaa aaaaaatcct catactctcc gccgccgccc tgatgctggc
     7861 agtctgccaa tccggcagcg cacctaaaaa cgcgcacacg gcatccgccg gcacaccggc
     7921 cggagaatca tgcaaagtca tccgggacga caccgcagga agcggcaaaa acatcattta
     7981 ccgttgctgc aacggtcagg cggcaataaa tgcagccatt gcagacggcg tattggaatc
     8041 agggattccc gtcagcttcg gcggcggggg cgtaccggtt gcaaacggca ggaatctggt
     8101 tacacggcaa gcagcaaacg gcgttggcaa atccgactcc gaggcctgcg aacacgccct
     8161 aatcaatgcc gcccgcaaat tccagcagac cgccggcaaa ctcggcgggc gcggcgttac
     8221 cggcttccac agctacttcg acaagcagcc gctgcaaggc ggacaatacg actgtcaggc
     8281 aggctcgttc cacgtccgtg tggtcatgcg cgccaatgtc gtccgttaac atatcggcaa
     8341 tcaaaaaatt aggctgtgtt ttagtaatct gttgatttca attatttgca aaggaaaaga
     8401 caattatttt ttcggttagg aagagaccta tcctattgaa tacatttaag ttaaatacgt
     8461 ctatcaacac tatattatag tggattaaca aaaatcagga caaggcgacg aag
//
LOCUS       DAXHPG010000083         8493 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXHPG010000083 DAXHPG010000000
VERSION     DAXHPG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.083"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(207..1592)
                     /gene="pntB"
                     /locus_tag="ACFM6F_001357"
     CDS             complement(207..1592)
                     /gene="pntB"
                     /locus_tag="ACFM6F_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC6164759.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1672..2055)
                     /locus_tag="ACFM6F_001358"
     CDS             complement(1672..2055)
                     /locus_tag="ACFM6F_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC6164760.1"
                     /translation="MTFAYWCILIAYLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFVCTVGLFVAAA"
     gene            complement(2059..3600)
                     /locus_tag="ACFM6F_001359"
     CDS             complement(2059..3600)
                     /locus_tag="ACFM6F_001359"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC6164761.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTSSEKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFAVTRRMLN
                     MFKKG"
     gene            3890..4723
                     /gene="serB"
                     /locus_tag="ACFM6F_001360"
     CDS             3890..4723
                     /gene="serB"
                     /locus_tag="ACFM6F_001360"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC6164762.1"
                     /translation="MPHALVLQFPSAEVLPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLPDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDERVLADVYENILKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLLEYR
                     SRLGLQPHQVLAMGDGANDILMLKETGIGVAYRAKPKVRAAADVCINFGGLERVRGLF
                     R"
     gene            4789..6012
                     /locus_tag="ACFM6F_001361"
     CDS             4789..6012
                     /locus_tag="ACFM6F_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6164763.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGAY
                     ISFREGVARASEGRRLGVLILCGMLAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLTV
                     STSLLWFSAVIGPILGATAVFFQRTAQKFPFIKRDNIRIILLAVCMFALIGVISVWFP
                     EVLGNGKAGNQLTFSGLTGWQYALELAAVKWPVVLMALAAGAYGGLITPSMMLGSTVS
                     FAAAAAWNSVFPAMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6172..7752
                     /gene="purH"
                     /locus_tag="ACFM6F_001362"
     CDS             6172..7752
                     /gene="purH"
                     /locus_tag="ACFM6F_001362"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC6164764.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPGCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAEME
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIYPAAGSLAAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRIGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDANLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
     gene            8365..>8493
                     /locus_tag="ACFM6F_001363"
     CDS             8365..>8493
                     /locus_tag="ACFM6F_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC6164765.1"
                     /translation="MLIHYIDIAKRNFVIAVSSLSKTKTETNNPKGIAHTIEYLKKH"
BASE COUNT         1907 a         2305 c         2378 g         1903 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 tcactgcaaa ccttcataaa cggggtttgc agtgattttt tcaaatcaaa cagattgaaa
      181 acctgcgccg aattgttcag acggcattat tttttcagtt cggacagaat gtcatctacg
      241 gttttcttcg catctccgaa acacatcacg ctgttttcgt tgaagaacag tgggttttgt
      301 acacctgcgt agccggtatt catcgagcgt ttgaagacga cgacttcttt tgccttccac
      361 acttccaaca cgggcatacc cgcaatcggg ctgttcgggt cggtttgggc ggcggggttg
      421 acggtgtcgt tcgcaccgat gaccaagacc acatcggttt cggggaagtc gtcgttgatt
      481 tcgtccattt ccaaaacgat gtcgtagggg actttggctt cggcgagcag tacgttcata
      541 tgaccgggca ggcggccggc gacggggtgg atgccgaagc gtacttcggt gccgttttta
      601 cgtaaaagct cggtgatttc ggcaacgggg tattgcgctt gtgcgactgc cataccgtag
      661 cccggggtaa tgatgacatt gtttgcgcct ttcagcattt cggcaatatc ggcagctttg
      721 acttctcggt attcccctat ctcttggctg ccggaagata atgtgccgct gtcgctgccg
      781 aaaccaccgg caattaccga gacaaacgag cggttcatgg ctttgcacat aatgtaggac
      841 agaatcgcgc cgcttgagcc gaccagcgcg ccggtaacga tgagcaggtc gttggagagc
      901 atgaagcctg ccgctgcggc cgcccagccg gagtaggagt tgagcatgga cacgaccacg
      961 ggcatatctg cgccgccgat ggaggcaacc aagtgccagc cgaatgcgag ggcaatcagg
     1021 gtcataatca gcaggatgaa gccgctgccg tcaatgccga caaatacgag cagcaacaca
     1081 aacgatacgg caagtgccag tgcgttgagc ttgtgtttgg cgggcagttg cagcgggctg
     1141 ctgctgattt tgccgttgag tttgccgaat gcgaccagcg agccggtaaa ggttaccgcg
     1201 ccgatgaaga tgcctaaata cacttcgacc agatggatgg tgtgcatatc gtgcgaaacg
     1261 ttgcccggcg cgatatagct gttgaagccg accaaaaccg ccgctaggcc gacgaagctg
     1321 tgcagcaggg caatcagttc gggcatttcg gtcatttcca cctttttggc tttgtagatg
     1381 ccgattgccg cgccgatgag catggcgatg atgatccagc ccagtccgtg ggtattgtcg
     1441 gaaaaaacag ttacaaaaag ggcgaccgcc ataccggcga taccggaata gcagccctgt
     1501 ttggcggttt cctgcttgga cagccccgcc agcgaaaaga taaataaaat tgcggcaacg
     1561 atatacgccg ctgttacgag tcctgaagac atggaaattc tccgattttc gatgatttgt
     1621 tttcaatgcc gtctgaaaaa ttgacgttcg tgttttcaga cggcatctgt ttcaagcagc
     1681 cgcgacaaac agcccgacgg tgcagacaaa tccgcccgcc cacatcagcg agcgcaatgc
     1741 tgctttgtcg gcgatgtagc accagataaa ggcgaggcgg aacaggatga acaggccggc
     1801 aagcgtgttg acggttgctt gtccggcatt gccggttgca tgtgccgtca aaacggcggc
     1861 agcaaacggt gcaaaggctt caaaaccgtt ttgctgcgcg gcgtgggcac gggcggctgt
     1921 gccttgcgtg cgcgccagaa aatcgcgcgg attgtggttg tctttaaacc ggaatccgcc
     1981 cgcttttttg gcatacgccg cacaaaaaag cggcaatagg taggcaatca gaatacacca
     2041 ataggcgaaa gtcatggctt accctttctt aaacatattc agcatacgcc gtgttaccgc
     2101 aaagccgccg aagatgttga tgctggcaat caggatggcg atgaaggcaa gcagggtaac
     2161 gaagccgttg ccttggctga tttgcagcag cgcgccgacg acgatgatgc cggagatggc
     2221 gttggtcacg gacatcagcg gggtatgcag cgagtggctg acgttccaga cgacgtagta
     2281 gccgatgacg caggcgagga cgaacacgat gaagtggttc aaaaatgctg cgggcgcgac
     2341 cgcgccgacc cacagcacca acacggcggc gatgacggcg ggcgcgagtt tcttccacag
     2401 gggaacgggt ttcggctccg gcttggcggc aggcgcggct ttttcagacg acgtttgctg
     2461 cggctgggcg gaaacttgaa tcggcggagg cgggaaggtg atttcgccgt cgcgggtaac
     2521 ggtcatgttg cggataatca cgtcttcgaa gtccaacgtg atttcgccgt ctttgttcgg
     2581 gcttaacagc ttggtcaggt tgaccaagtt ggtggcgtaa agctgggaag actgtccggc
     2641 aaggcggttt gccatgtcgg tgtagccgat gattttcacg ccgttgccgg ttacggacaa
     2701 ttcgcccggg cgggtgagtt cgcagttgcc gcccgtcgcc gccgccaaat cgacgatgac
     2761 ggagccggat ttcatgcttt ccaccatttc tttggtaatc agcttgggcg cgggtttgcc
     2821 cggaatggcg gcggtagtga tgatgatgtc cacttctttt gcctgctcgg caaagagctt
     2881 catctcggcg gcgataaatt cgtcgctcat cactttggcg tagccgtcgc cgctgccgcc
     2941 cgattcttgc gggaagtcga gtttcaggaa cttgccgccc atcgattcga tttgttccgc
     3001 cacttccaag cgggtatcga acgcgcgtac cactgcgccg agcgagtttg ccgtaccgat
     3061 cgccgccaaa cccgccacac ctgcaccaat caccaaaacc tgcgcgggcg gcactttgcc
     3121 ggcggcggta atttgaccgg tgaagaaacg gccgaaggcg ttggcggctt caatcacggc
     3181 gcggtagccg ctgatgtttg ccattgaaga caaagcgtcc aaagcctgcg cgcgcgaaat
     3241 gcggggcacc atatccatcg ccagcgcgtt cactttcttg gcgcgcaagg cttcgaccaa
     3301 agcctcgttt tggcgcggcc acaggaagct gacgatggtt tgaccttcgt tcaaaagcgg
     3361 cagttcctgt tcggacggcg cgttgacctt ataaatcaaa gggcaggccc aaaccgccgc
     3421 tttgtcggca acggttgcgc ctgctgtttg gtaagcggca tcgtccaaac ttgccgccaa
     3481 acctgcaccg ctttcgacaa cggtttcaaa gcccagtttg cccaacaggg caacggtggc
     3541 gggcgtgcag gcgacacggg tttcgccgga taatgactcg cgtgggatac cgattttcat
     3601 ctctgaatcc tttttcgggt tgtttatatg tatcgtgggt taaatttaaa tcggggcagg
     3661 gcggagcaac gccgtaccgg tttaaagccg actcacttca aatgttaata tattttagat
     3721 aatcccctta taacgaattt tcatcaggct ggcaatagtt gcggcatttt cccgtgttgt
     3781 ccgacacata ctgccgtttg cgtttgaagt cattttccga ttgcagtccc gcccgccgta
     3841 acattctttg ccgtttcact atataatccg cattttttga gccgccgtta tgccgcacgc
     3901 cctcgtcctc caatttccct ccgccgaagt cctgccttcc gacttcccct cacgcctgcc
     3961 cgaacctgat tgcgccgatg aaaagcgtat gcgttttatc gttgaagaag ggttttcttt
     4021 aagcgaaaaa gacgcggcgt tgcttggcag ccgtcaaatc gaccacgccg tgttgccgga
     4081 tatggatttc ggcgaactcg gtttgattgt cagcgatatg gattcgacgc tgattaccat
     4141 cgaatgcgtc gatgaaattg cggcaggcgt gggtttaaaa gacaaagtag cggaaattac
     4201 cgagcgttcg atgcgcggcg aactcgattt cgaacagtct ttacgcagcc gcgtcgcgct
     4261 gttggcggga ttggacgaac gggttttggc ggatgtttat gaaaacattt tgaagctctc
     4321 gcccggtgcg gaatttttgt tggacgaatg caaaaggcac gatgtgaaat tcctgctggt
     4381 gtcgggcggc ttcacgtttt ttaccgaaag gctgcaacaa cgcctcggct tcgaatacca
     4441 gcacgccaat gttttggaaa ttgaaaacgg caggctgacc ggccgtctga aaggtagaat
     4501 catcgacgcg caggcaaagg cagatttatt gctcgaatac cgcagccgcc tcggattgca
     4561 gccacatcag gtgttggcga tgggcgacgg tgcgaacgat attctgatgc tcaaagaaac
     4621 gggcataggc gtggcttacc gtgccaaacc gaaagtgcgg gcagccgccg atgtctgtat
     4681 caacttcggc ggtttggagc gtgtacgcgg cctgttcagg taggcggata ggaaacggat
     4741 gtcgtctgaa aaacgtgttc agacggcatt tgaacggcag gaacgacagt gggacgcaga
     4801 aaactttggt ttgccctggc agcagcaggc gttatcggcg gtttggtcgg cattgtgctg
     4861 acggaactga tgcacttcat acagcatacg gcatacggtt atggcgcgga cggtgcgtac
     4921 atctcgttcc gcgaaggtgt ggcccgtgct tcggaagggc ggcgtttagg cgtattgatt
     4981 ctgtgcggta tgttggcagg cggcggctgg tggttgctca aacgtttcgg taagccgcaa
     5041 atcgaaatca aagccgcctt gaagcagccg ttgcaggggc tgccgtttct gacgacggtt
     5101 ttccatgttc tgctgcaaat cataacggtc ggactcggtt caccgctcgg acgggaagtc
     5161 gccccgcgtg aaatgactgc cgcgttcgct tttgccggcg gcaggcggtt gggtttggat
     5221 gaagacgaaa tgcggctgct gattgcttgc gcttcgggtg cgggtttggc ggccgtgtat
     5281 aacgtgccgc tcgcctccac actttttatc ctcgaagcca tgctgggcgt gtggacgcag
     5341 caagccgtcg ccgccgcatt gctgacttcc gtcatcgcca ccgccgtcgc gcgcatcggc
     5401 ttaggcgacg tgcagcaata tcatccggct aaccttaccg tcagcacctc attactttgg
     5461 ttttccgccg tcatcggccc gatactgggc gcaaccgccg tctttttcca gcgcacagcc
     5521 caaaagtttc cctttatcaa gcgcgacaat attagaatca ttctcttggc agtctgcatg
     5581 tttgcgctca tcggcgtgat ttccgtttgg tttcccgaag ttttgggcaa cggcaaagca
     5641 ggcaaccaac tgacttttag tggtttgacc ggttggcaat acgctttgga acttgccgcc
     5701 gtcaaatggc cggtcgtctt gatggcgctg gcggcaggcg catacggcgg actgattacg
     5761 ccgtcgatga tgcttggcag caccgtttct tttgctgccg ccgccgcgtg gaacagtgtt
     5821 ttccccgcaa tgccgtctga aagcgcggca gtcgtcggag cggcggtgtt tctcggcgta
     5881 tcgctcaaaa tgccgctgac cgccattgtg ttcgtattag aactgaccta cgcgccgctg
     5941 tccctgctgc tgcctttgtg catcggcatg gcgggggcat tgggaacatc gggaaaaatg
     6001 ggcttcaaat gaatgtcgac aaatgccgtc tgaaaggttt tcagacggca tttctttgta
     6061 aattgtcaac aatatctgca aaaatctact gccaaaaaac tgaaaatagc ggataatacc
     6121 gccccgaaaa agttcccctt tccccatcca actgtcagaa aaggaagccc aatgtccgtc
     6181 atcaaacgtg ccctgatcag cctgtccgac aaggcaggcg cggtcgaatt tgcccaaacc
     6241 ctgcacaagc tcggtgtcga aatcctctcc accggcggca cggcgaaact gcttgccgat
     6301 gcgggcgttc ccgtgattga agtcgccgac tataccggct tccccgaaat gctcgacggc
     6361 cgcgtgaaaa cgctgcatcc gaaaatccac ggcggtattt tgggtcggcg cgatttggac
     6421 gaacacgttg ccaagatgga agaacacggt atcggcaaca tcgaccttgt gtgtgtcaac
     6481 ctctacccct tcgccgccac cattgccaag cccggctgca cgctggaaga cgcgattgaa
     6541 aacatcgaca tcggcggccc gaccatggtg cgttctgccg cgaaaaactg gaaacacgtc
     6601 gccatcgtta ccgacacagc cgatttcccg gccatagctg ccgaaatgga agccaacaac
     6661 ggcgcattga gcgacaaaac ccgcttcaac ctctcacgca aagcattcag ccataccgcc
     6721 caatacgacg gcatgatttc caactacctg acctcgcttt cagacgacgt cttgagcggc
     6781 cagccccaaa tcggcgaatt cccaagtcgg ttcaaccaaa gctggattaa agtgcaagac
     6841 atgcgctacg gcgaaaatcc gcaccaacgc gccgcgttct accgcgatat ttaccccgcc
     6901 gcaggcagcc tcgctgcata caaacaactg caaggcaaag aattgtccta caacaacatc
     6961 gccgatgccg atgccgcatg ggaagccgtc aaatcctttg acgcaccagc ctgtgtgatt
     7021 gtgaaacacg ccaatccgtg cggcgtcgcc gttgcagccg atacattgac cgcctacaaa
     7081 ctcgcctacg tcaccgacac caccagcgcg ttcggcggca tcatcgcctt caaccgcgaa
     7141 gtggacggcg aaaccgtcaa acagattacc gacaaccagt ttatggaagt cttgatggcg
     7201 ccgaagttta ccgccgaagc cctcgaaatc gccgccgcca agaaaaacgt gcgcgtattg
     7261 gaagtgccgc ttgaggcagg cgcaaaccgc ttcgaactca aacgcatcgg cggcggactg
     7321 ttggtacaaa cccccgacat ccaccgcatc agccgcgccg atttgaaagt cgtctccaaa
     7381 cgccaaccga ccgagcagga atggaacgat ttgctgtttg tctggaacgt cgccaaatac
     7441 gtcaaatcca acgccatcgt cttcggcaaa ggcggtcaaa cctacggcat cggcgcaggt
     7501 caaatgagcc gcgtggacag cactcgcatc gccgctcgca aagcgcaaga tgccaatctc
     7561 gacctcaatg gcgcatgtgc cgcttccgat gctttcttcc ccttccgcga cggcgtagat
     7621 gtcattgccg agcagggcat caaagccatc atccatccgg caggctccat gcgcgatcag
     7681 gaagtcttcg acgcagcgga cgaacacggc atcgccatgg tcgtaaccga cgtgcgccac
     7741 ttccgccatt aacgcagatg aacaaggcaa tgccgtctga agggctttca gacggcattt
     7801 tgcgctattt tgtgaaggta gggatgacgg ttcgggtatt cctgacaggg tggattttca
     7861 aggtgttgta tagggtgtag gaggattcgt aaaaggtggg atgcagggtg tgcttcagcc
     7921 cgctgcatca aaaatttttg gagaaccggc gggagtcggc ggttttggtt tcggcgggga
     7981 cggtggaaat gggtaacatt gacggaatcg acggaagcgg tgggctgaag cccacccttg
     8041 tatattggaa tcactgtatc atagcaacaa accgcccggc cgccacccgc gcccacccaa
     8101 ggcacacaac cgttgcgtag cacagggagc ggcagggcaa cccatcgaca caaccggaca
     8161 gttgccggac aacacaaccg aatgcaaggc aggtttatga tgagtaccca ataccattac
     8221 gcaggtatag tgaattaaat ctaaaccagt acagcgttgg ttcgccttag ctcaaagaga
     8281 acgattctct aaggtgctga agcaccaagt gagtcggttc cgtactatcc gtactgtctg
     8341 cggctcgccg ccttgtcctg atttttgtta attcactata tcgacatcgc caaacgaaac
     8401 ttcgtcatcg ccgtttcgtc tttgtctaaa accaaaaccg aaaccaacaa ccccaaaggt
     8461 atcgcccata ctatcgaata ccttaaaaaa cac
//
LOCUS       DAXHPG010000084         8408 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXHPG010000084 DAXHPG010000000
VERSION     DAXHPG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.084"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(1..146)
                     /locus_tag="ACFM6F_001364"
                     /pseudo
     CDS             complement(1..146)
                     /locus_tag="ACFM6F_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(308..1240)
                     /gene="cysK"
                     /locus_tag="ACFM6F_001365"
     CDS             complement(308..1240)
                     /gene="cysK"
                     /locus_tag="ACFM6F_001365"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC6164766.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVPNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1434..2039)
                     /locus_tag="ACFM6F_001366"
     CDS             complement(1434..2039)
                     /locus_tag="ACFM6F_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164767.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(2036..2887)
                     /gene="dapF"
                     /locus_tag="ACFM6F_001367"
     CDS             complement(2036..2887)
                     /gene="dapF"
                     /locus_tag="ACFM6F_001367"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC6164768.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVEQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            3024..3320
                     /locus_tag="ACFM6F_001368"
     CDS             3024..3320
                     /locus_tag="ACFM6F_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164769.1"
                     /translation="MFRILLLLFPCAILAACARNIPASPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3334..4002
                     /locus_tag="ACFM6F_001369"
     CDS             3334..4002
                     /locus_tag="ACFM6F_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6164770.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(4075..6195)
                     /gene="pnp"
                     /locus_tag="ACFM6F_001370"
     CDS             complement(4075..6195)
                     /gene="pnp"
                     /locus_tag="ACFM6F_001370"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC6164771.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6432..7295)
                     /locus_tag="ACFM6F_001371"
     CDS             complement(6432..7295)
                     /locus_tag="ACFM6F_001371"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC6164772.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7495..8358
                     /gene="rfbD"
                     /locus_tag="ACFM6F_001372"
     CDS             7495..8358
                     /gene="rfbD"
                     /locus_tag="ACFM6F_001372"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC6164773.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         2005 a         2421 c         1948 g         2034 t
ORIGIN      
        1 gtggattaac aaaaaccagt acagcgttgc ctcgccttgc cgtactatct gtactgtctg
       61 cggcttcatt gccttgtcct gatttttgtt aatccactat atttgtactg tctgcggctt
      121 cgttgccttg tcctgatttt tgttaatcca ctataaaaaa tatttccctc aagcgatgca
      181 gcgacaccaa cacctgatta aaacacaacc ggcgttttcg gcatatacgc aaaaacgcat
      241 tttccggcac ggcgttacac cgataccgaa aaatgcgtct gaatgtttcg acaatccgat
      301 taaagcatta tgccaaatct gcaaaaagtg gcgtagagag atagcgttcg ccataagaag
      361 gcagcagcac gactatcagc ttgccttcgt tttcaggctg tttggcaagc tgcaacgcac
      421 tccaaaccgc cgcaccggaa gaaataccca ccaaaatgcc ttctttttcc gctattgcgc
      481 gggcggtttc aaaagccgct tcgttcggca ctttggtaat gctgtcgtaa attttggtat
      541 tcaaaacggt cggaataaag cctgcgccga tgccttgaat cgggtgcggg cctttttcgc
      601 cgccgctcaa tacgggtgaa gcttcaggct cgacggcaac cactttaact tcgggtttgt
      661 attttttcaa cacttcgccc acgccggtaa tcgtaccgcc cgtgccgacg ccggcaacga
      721 agacatcgac tttgccgtcc gtatcccgcc aaatttcctc ggcggttgtt ttgcggtgga
      781 cttcggggtt tgcctcattg tcgaactggc gcggcataaa ataagtgtcg ggatgcgcgt
      841 ccaccaagga tttcgctttg gcaatcgcgc ccgccatacc ttcggcggca ggggtcagaa
      901 tcagctccgc accaaacgcg cgcaacagca ttttgcgctc cttactcatg ctttccggca
      961 tggtaatcgc cagcttgtag ccgcgtgcgg cacataccat tgccaaaccg acacccgtat
     1021 tgccgctggt tgcttcgaca atgacggtgt ttttgttgat tttgcccgct ttttcggcac
     1081 cctcgatcat tgcttcggca atgcggtctt tgacgctgct gcctggattg aaaaattcca
     1141 gtttcacggc aacctccgcc ttcaaacctt cggtcagacg gttcagtttg accaaaggcg
     1201 tgttgccgat tagttcggtg atgctgtttg caattttcat cttctgctcc tgaataataa
     1261 ggtttcgaca gtaaggcgca atccccatag gcatcctgtc cgattgcagg ggatttgcgc
     1321 cgaaacataa acaaatttcg gatatggctt tatcctaaac ctatgccgcc taaaaaacca
     1381 atatccaaaa tttctttctt tataactttt tgttccgata cggccgcacc gtattatctt
     1441 ccaatttctg aaagcagcag caccagtacc gagacctgca tcaacgctgc cgtcaacccg
     1501 ccgagtcgca gctgcgagcc gccgaacgtt gacggcggct gccggtctat accgcagcgg
     1561 tggacatagg caaccgccat cagcacaaac tgcaacacga accaataaac cggcttcagc
     1621 gcataaagtt tgagcagtgc cgcaaacaca aacacttgaa ccgttcccga aaagcaataa
     1681 tgcaccaacg cacatattcc gacaaaagca agcgtcatcg atacattact gacggcaaac
     1741 agcccgagca gaggatgttc ggggtctgcc tgccatccgt ttccatccgt tttccggttc
     1801 caatgcccga tgaaaaaaaa tatgctgccc aaagtcaggt aaaaatgggg gatgaacaag
     1861 ggcgcggcgc gggtcattcc ccatatgccg ggcatcagct ttgcccccaa aaccgatatg
     1921 cccagccaca gcataatact cgcccaaaac cattgcaaca ttcctgcgcg gaatatcaga
     1981 aacagaaaaa ccgccgcata cagtaaggca gacaaaaaat ccaaatgcag caaaatcatg
     2041 aatacgccaa ctcgccttca aacaccgctt ccgcagggcc ggtcatcatc acatcgccgc
     2101 cgcaggccca ttcgatatat aaagtcccgc ccggcaaaat cacctctacc gttttccctt
     2161 catccaacaa ccccagacgg atacccgcca ccacagccgc acacgcgccc gtaccgcacg
     2221 cttgggtttc gcccacgccg cgctcgaaca cgcgcaaacg aatcgcggtt cggctgacaa
     2281 tctgcataaa gccgacgttg acgcgttcgg gaaactgcct gtgcggttcg ataagcgaac
     2341 cggtttcgcg caccggcgcg cattctacgt catcgaccac aatcaccgca tgggggttgc
     2401 ccatattgac gcagctgaca ggctgaatgc cggattcgag atgcaccccg taaatacagg
     2461 catcatcccc ctcgcccgat tcggggacaa acggtatttc agacggcata aacctcggtt
     2521 tgcccatatt gaccgtaacc atgccgttat cggacaattt cggaaaaata acgccatttg
     2581 ccgtttcaac acaaatttct ttcttatcgg tcaaaccctt gtctgcaaca aaacgggcaa
     2641 aacaacgcgc tccgttgccg cattgctcga cctcgctacc gtcggcattg aaaatacggt
     2701 aacggaaatc cacgccttcg gtttccgaac gcccgaccac caaaagctgg tcgaagccca
     2761 cgccccggaa gcggtccgcc catgccgcaa tcggcgcgtc ctcgggggta aaatcctgac
     2821 tgaccgcgtc aatcaccata aaatcgttgc ccaaaccgtg cattttggta aatttcaaag
     2881 tgttcatatt gttccgaaat cccgtatcgt ctgttgcttc cgaatccgaa atcccccgca
     2941 ccgaagggaa atgcttccat ctcccgccgc ctgaggatta aaatcagcca ttatactgtt
     3001 tgttacccct tacaagaaac accatgttcc gcatattact gttgcttttc ccttgtgcaa
     3061 tccttgccgc ctgcgcccga aacataccgg caagccccgc cgtttcaaat tgcgcgcatc
     3121 ttcaaaaccg caccatcctg tattttgacg cacacggcaa acccgccgac ggcgcagtaa
     3181 agggcggatt ttacaaaatt atcgaaatac agggtgcaaa ctgcatcctt attcaagact
     3241 ttaacagcac gggcaatgcc ctcggcggat cctatgtgcc ggactgccgc aaatcatccc
     3301 ttttcccgaa aaatatttga cttcaaactg cctatgaacc acgacatcac tttcctcacc
     3361 ctgttcctac tcggtttctt cggcggaacg cactgcatcg gtatgtgcgg cggattaagc
     3421 agcgcgtttg cgctccaact ccccccgcat atcaaccgat tttggctgat cctgctgctt
     3481 aacacaggac gggtaagcag ctatacggca atcggcctga tactcggatt aatcggacag
     3541 gtcggcgttt cactcgacca aacccgcgtc ctgcagaata ttttatacac ggccgccaac
     3601 ctcctgctgc tctttttggg cttatacttg agcggtattt cttccttggc ggcaaaaatc
     3661 gagaaaatcg gcaaaccgat atggcggaac ctgaacccga tactcaaccg gctgttaccc
     3721 ataaaatcca tacccgcctg ccttgcggtc ggaatattat ggggctggct gccgtgcgga
     3781 ctggtttaca gcgcgtcgct ttacgcgctg ggaagcggta gtgcggcaac gggcgggtta
     3841 tatatgcttg cctttgcact gggtacgctg cccaatcttt tggcaatcgg cattttttcc
     3901 ctgcaactga aaaaaatcat gcaaaaccga tatatccgcc tgtgtacggg attatccgta
     3961 tcattatggg cattatggaa acttgccgtc ctgtggctgt aagccccatt cattcccgat
     4021 acccgtaaaa aataccgtct gaaacctgtt cagacggcac tttcacccta agcgttactc
     4081 ggcggcattt tcctcacggg caggcgcgtc cagcagggct ttgatggaca gacggacacg
     4141 gcctctgtcg tccacttcca atgctttcac gttcaccacc tgaccgactt gcaggtagtc
     4201 gccgacattg cgtacgcgct cgtgggcgat ttggctgatg tgtaccaaac cgtctttgcc
     4261 cggcatcacg ctgacaatcg cgccgacgtt gttgtcgagg attttcacca cagtgccttc
     4321 atacactttg cccacttcca cttcggcagt aatctgctcg atgcgttttt tcgccgcatc
     4381 gccggcttct tgagtggtcg cggcaatggt aatcgtaccg tcttcggcaa tattgatttc
     4441 cgtaccggtt tcagcggtaa tcgcacggat ggtttcaccg cccttaccga taacttcgcg
     4501 gattttgtct tggttgattt tcatcgtaaa caaacgcggc gcgtgtgcgg acaactcttg
     4561 cgggcccgca acggcggctt tcatctgatc caagatgtgc agacgcgctt ctttggcctg
     4621 tgccaaagcg atttgcataa tttctttggt aatgccttgg attttgatgt ccatttgcag
     4681 cgcggtaacg ccttcggtcg taccggccac tttaaagtcc atatcgccca agtggtcttc
     4741 gtcgcccaaa atgtcggtca ggacggcaaa tttgttgcct tccagaatca gacccatcgc
     4801 gataccggca acgtgtgctt tcaaaggcac gccggcagac agcaggctca aacagccgcc
     4861 gcagacggaa gccatagagg aagagccgtt agattcggta atttcggaga ccacgcgcat
     4921 ggtgtagctg aaatcttcag gtttcggcaa tacggccaac aatgcacgtt tagccaaacg
     4981 gccgtgaccg atttcacggc gtttcggcgc gcccatgcgg cccacttcgc cggtagagta
     5041 cggcggaaag ttgtagtgca gcataaagcg gtcggtgtat tcgccggaca gcgcgtcgat
     5101 gatttgctcg tcgcgcgaag tacccaaagt cgctacggca agggcttggg tttcgccacg
     5161 ggtaaacaat gcggaaccgt gcgtgcgcgg caatacgccg gtttggatgt tcagcggacg
     5221 gacggtgcgg gtgtcgcggc cgtcgatgcg cggttggccg tccaaaattt ggctgcggac
     5281 gacatcggct tccaagtgtt tgaaaatgcc tttgatttcg ttggctgcca gcgtgtcggt
     5341 ttcttcggta atcaaggctt cttttaccgc actccaagct tcgtccaatt tggcagaacg
     5401 cgcttgtttt tgacggattt tgaacgcttc tttaatggtt tcgccggcaa tcccgcggac
     5461 tttggcaacc agttcctcat tggtttcagg tgctttccaa tcccaaagtt ccggattgac
     5521 ttcgtcggca aattcattga ttgcattgat ggcaacctgc atttgatcgt ggccgtaaac
     5581 caccgcgccc agcatcacgt cttcgggcag gattttggct tcggattcca ccatcaacac
     5641 ggctttggaa gtaccggcga ccaccaagtc caattgcgat ttcgccaatt cggctttagt
     5701 cgggttcaaa acgtacacgc cgtttacata accgacgcgt gccgcgccga tcgggccggc
     5761 aaacggtacg ccgctcaaca ccagcgcggc agacgcgccc aacattgcag gaatatcaga
     5821 atcgatttca ggatcgacgg acacgaccat cgctacgatt tggatgtcgt ggtagaaacc
     5881 ttcagggaac agcggacgaa tcggacggtc gatcagacgg ctggtcagga tttctttttc
     5941 gctttgtttg ccttcgcgtt tgaagaaacc gccgggaatt ttgcctgcgg cgtaagtgcg
     6001 ctcgaggtaa tcgacagtca gagggaagaa gtcttggcct tctttcactt ctttgttggt
     6061 ggtaacggca accaaaacca cggtgtcgcc catagagact ttaacggcag cggcggcttg
     6121 gcgggcaatt tcgccggttt ccaaagtaac ggtgtggttg ccgtattgga aagttttaac
     6181 gtgtttgtcg aacattattg ttcctttcaa aataccgcac tgctaaaaca ctaataatgc
     6241 acactaaaat ccgaatgtgc atagttaggg tttcagaccg tgcggcaggt tgtaaacaag
     6301 ctttcagacg acatttcgga cgctgaaagc aattccaaat tataaaggca accatcatta
     6361 aaatccagta ttgatacaat aaaaaggccg tctgaaccat atttctttca gacggccttc
     6421 aaacttaaaa atcaatcctg agtcagcaaa gtcaatgctt cccgatactt ctcgacagtt
     6481 ttctgaatca catcggcagg cactttcggc gcaggggctt ttttgttcca accgctttgt
     6541 tccagccagt cgcggacgaa ttgtttgtca aaagacggcg gattggtgcc gactttgtat
     6601 tggtcggcag gccaaaaacg gctcgaatcg ggagtcaata cctcatccat cagcgtcagc
     6661 gtaccgtttg tatccaaacc gaattcaaat ttggtatcgc aaatgatgat gccgcgcgat
     6721 ttggcatatt ccgccgcttc tgtataaagc tgaatcgctt tggcgcgcac ttccgccgcc
     6781 aattcttttc cgataatgcg ttcgcattct tcaaagctga tgttttcatc gtgatcgccg
     6841 actgcggctt tggttgaggg cgtaaaaatc acttcaggca gttgttgcgc ttcctgcata
     6901 ccttcaggca gttgaatacc gcaaaccgag ccggtttttt gataatcttt ccaaccgctg
     6961 cctgccagat aaccacgcac aatcgcctct actttcaccg gagtgagctt tttagccacg
     7021 acggcgcgtt tctctaaagc tttggcttcg ttttcaggca aaacatcgta aaccgtttga
     7081 ccggtaaagt ggttgggcat aatatgcacc agttttttaa accaaaaatt ggaaatctgc
     7141 gtcagaatct cccctttgct cggaatcggg tcgtccaaaa tcacatcgaa tgcagacagg
     7201 cggtcggaag cgaccatcag catacgttta tcgtcgattt catataaatc gcgcactttt
     7261 ccaaaataga tctttaccaa accaatctca ctcatttcgc ccccccctga aaatatcttg
     7321 aaaataccga cccgacaccc gacaggtttg aatcacaaac caatattcta gccgaagtcg
     7381 gcgcaaaaca atacccatgg cacaaaaaac caacccgtca accgtcggca aagttttggc
     7441 actataatac cgacagcaag tcctacaata cacttttacc aaaggaaata cctcatgaga
     7501 atcctattga caggctcgaa aagccaactg gcacgctgcc tgcgcgaccg tcttccggaa
     7561 gactgggaaa ccattgcgac ggattccgca tccctagaca ttaccgatgc cgatgccgtc
     7621 tgcaacatgg tcaaaagttt ccaacccgac gccattgtca acacggctgc ctatactgcc
     7681 gtcgacaaag cggaaggcaa tgcggcagcg gcatttgccg tcaatgcttc cgccgtttac
     7741 aaccttgcct tggcagcaca tcgcgcccat gcccgattca tccacatctc aaccgactat
     7801 atctttgacg gtaaagggaa aaggccctat caggaaagcg actttaccaa tccttccaat
     7861 gtatacggac aatccaaaac cgcaggcgag ctgctcgcac tgtctgccaa tcccgacagc
     7921 cttatcctgc ggacttcttg gctgtttagc gaatacgggg acaactttgt ccgcacgatg
     7981 ctgaaccttg cgcgggaacg ttccccgctg tccgccgtcc acaaccaaat cggctgcccg
     8041 acctatgccg gcgatttagc tgccgccatc atccgccttt tgcagcaaac gtcgcccgtg
     8101 cgcggcattt accactacgc cggcggcaaa tccgtatcct ggtatgaatt tgcccaacat
     8161 attttccaag cggcatcgca acagcagaca tccttccccg ttcccgaatt gactgccgtt
     8221 tcagacaagg aatatccgac cgccgccccc aggcccgcat acagcatttt ggactgccgc
     8281 aaaatcgaaa acgacttcgg catcaaaccg tcagactggc aaaaagccct tgcgcaggtc
     8341 gtttccaagc tgctctgatg ccgcccgcct ctccgtttcc gccgtcaagc accgccttgg
     8401 cggttttc
//
LOCUS       DAXHPG010000085         8273 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXHPG010000085 DAXHPG010000000
VERSION     DAXHPG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.085"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(108..1031)
                     /gene="era"
                     /locus_tag="ACFM6F_001373"
     CDS             complement(108..1031)
                     /gene="era"
                     /locus_tag="ACFM6F_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC6164774.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1078..1797)
                     /gene="rnc"
                     /locus_tag="ACFM6F_001374"
     CDS             complement(1078..1797)
                     /gene="rnc"
                     /locus_tag="ACFM6F_001374"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC6164775.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            1964..2287
                     /locus_tag="ACFM6F_001375"
     CDS             1964..2287
                     /locus_tag="ACFM6F_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC6164776.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2335..2811
                     /gene="ribH"
                     /locus_tag="ACFM6F_001376"
     CDS             2335..2811
                     /gene="ribH"
                     /locus_tag="ACFM6F_001376"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC6164777.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2889..3314
                     /gene="nusB"
                     /locus_tag="ACFM6F_001377"
     CDS             2889..3314
                     /gene="nusB"
                     /locus_tag="ACFM6F_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC6164778.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3681..4715)
                     /gene="pyrC"
                     /locus_tag="ACFM6F_001378"
     CDS             complement(3681..4715)
                     /gene="pyrC"
                     /locus_tag="ACFM6F_001378"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC6164779.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVVPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATSELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFTSKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4775..5380)
                     /locus_tag="ACFM6F_001379"
     CDS             complement(4775..5380)
                     /locus_tag="ACFM6F_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164780.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSCVRHQDFARVAVDLF
                     RSACVLTQGSFEPVSKFAAVGHFVPVGKEDLSRLPPAVAEPDVQALWTLERDGGIYYL
                     SLPRDCCSVKAERADSAVLLEHFDGLVRQPPKNTNIELRAEQSVQALFETRQISYAWR
                     ESGSPEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            complement(5381..5605)
                     /locus_tag="ACFM6F_001380"
     CDS             complement(5381..5605)
                     /locus_tag="ACFM6F_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC6164781.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVKHK"
     gene            5714..6325
                     /locus_tag="ACFM6F_001381"
     CDS             5714..6325
                     /locus_tag="ACFM6F_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC6164782.1"
                     /translation="MRRTALLLLALTAGTSLAAGFNQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSMKPHDK"
     gene            complement(6385..7257)
                     /gene="accD"
                     /locus_tag="ACFM6F_001382"
     CDS             complement(6385..7257)
                     /gene="accD"
                     /locus_tag="ACFM6F_001382"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC6164783.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPESFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(7294..8079)
                     /gene="trpA"
                     /locus_tag="ACFM6F_001383"
     CDS             complement(7294..8079)
                     /gene="trpA"
                     /locus_tag="ACFM6F_001383"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC6164784.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
BASE COUNT         2009 a         2492 c         1968 g         1804 t
ORIGIN      
        1 atgcacagat aatatcaacc cgtttttaac aaagatattc ccgattgaaa caaaatgccg
       61 tctgaaacct gttcggacgg catttgcgta aagttcagca agaaaaacta caaacccagc
      121 tcgcgcagga agcggatgtc gtccgcccaa ccggatttga ctttgaccca gaccttcaaa
      181 aatactttgg tatcaaacag tttttccata tccaaccgcg cttcggtgga aattttcttc
      241 aaacgctccc cgcctttgcc gattaaaatc gccttttggc tttccttatc gaccaaaacg
      301 gcgatataga tgcggttcaa accgtcttcc tcttcaaact gctccacttc gacgttcatc
      361 gcataaggca attcctcgcc caaatagcgg aacaattttt cacgcacgat ttccatcgct
      421 aaaaaacgcg ccgatttgtc cgtaaccata tcttcgggat acatcggcac gctttcgggc
      481 agatacggct tgagcagctc caacaggttg gcaatccgca atccgtgttt cgcactgacc
      541 gcctccgccg ccgcaaattc aaattcggcg cgcacttggg caacaaacgc ctccagcgcg
      601 taacggtctt tcgccttgtc cttgtcgatt ttgttgacca ctaaaatgac cggcgtgtgc
      661 ttgggcagtt gtttcaacac gacacggtcg gcatcggtaa agcgcatcgc ttccacgacg
      721 aaaaccacca catccacgcc gccaagcgcc tcggtaacgt tttgattcag cctgtcgttg
      781 agcgcgttgc ggtggtcggt ttgaaagccg ggcgtatcga caaacacgaa ctgcgcggta
      841 tcgtcggtat aaatccccgt tacgcggttg cgcgtcgtct gcgccttttt gctggtgatg
      901 ctgattttct gaccgatgag atggttcatc agcgttgatt tgcccacgtt cggacgaccg
      961 acaatcgcca cgaagccgca acggtatccg ccggcggcgc gttcccctgc aaggaaggtt
     1021 tcaatatcca tattcgcttt catatccaca tttcagacgg catattcacg cgccgcctca
     1081 tttctttttc ttcttcagcg gcagcttctc ttccagccat ttcaaagcct ctttcgccgc
     1141 ctcctgttcc gccgccttgc ggctcgtccc tttggcacgg cacacgaaac ccagttcgcc
     1201 caaatcgcag gaaaccacaa acatactgtc gttggcatgg ccgatttgct cttcgatacg
     1261 gtatttcggc aaagcaaagc ggcgtgcctg caacgcctcc tgcaaagcag ttttgccgtc
     1321 ttttgcctga ttttggaaat cggcacaccg gacgcggtcg gcaaacaaat ggcgcaccac
     1381 cttttccgcc gtgttgaaat cggcatcgaa gctgaccgcc gcaaacatcg cctccatcgc
     1441 gtccgccagt atcgaaggcc gtctgaagcc gccgctcttc aactcgcccg cccccaagta
     1501 cagaccgtcg ccgacattca tttccgccgc catttccgcc agcacgccct cattgaccag
     1561 acttgcccgc aaccgcgaca actcgccctc ggtcaacttc ggaaacgtgt caaacagcat
     1621 ccgcgccacc gtataattca aaatcgaatc accgacaaac tcgaaccgct cgttgtgctt
     1681 cgcatgatgg ctcctgtgcg tcaaagcctg ccgcaaaagc gaaatatcgc ggaacgcgta
     1741 gcccagtctc ttctgtatcg ccgcgtgtgc ctgctgtttc aaaacatcgt ctttcattac
     1801 cgtcttccgc aaaaaacagc ccgaatgcgt ttccatactc caaaaataca gaaacacctt
     1861 aaggctgcaa acggcgcaca ttgtaccacc aaaccgccgc cgtctgccga caacgcacga
     1921 atcgtggtaa aatccgtaag ttttccccca acgtattccc gtaatgaacg catttgcaca
     1981 agcactttcc tcggcactcg accgctgtat cgccaccaac accgtcgcca aacaaaaccg
     2041 gcccgtcggc ttcctctacc gcgaagcccc cgtttttgaa aacgacagcg gctggcgttt
     2101 cttcagcggc gacgagaccg acgaatacac cgacgatccc gacaacttca gcatcgtcag
     2161 cctcgccgac atcgccaaaa ccaaccctga aaccgccgca ctgctttccc aacccgaagg
     2221 cagcgcatgg gagcttgccg aagacggcac attccaaacc gtcgccgact ggcagccgca
     2281 agactgaaac cttcagacgg cattccgaaa cctccgaata ccaaaggaca cccaatgaac
     2341 accatcgccc ccaacctcga cggcaaacac ctccgcatcg gcatcgtaca ggcacgtttc
     2401 accaacgaaa tcggcagcga aatgctcaaa gtctgctgcc gcaccctcca agaattgggc
     2461 gtggcagacg aaaacattac cgtcgccacc gtacccggcg cacttgaaat ccccatcgca
     2521 ctgatgaacc ttgcctcttc cgaaaagttt gacgcactga ttgccatcgg tgtcgtcatc
     2581 cgtggcgaaa cctaccattt cgagctggtt tccaacgaat ccggagcagg ggttagccgc
     2641 gtcgcactcg actacaacat cccgattgcc aatgccgtcc taaccaccga aaacgacgcg
     2701 caggcaatcg aacggattga agaaaaagcc tcggatgccg ccaaagtcgc cgtcgaatgc
     2761 gccaacctcg tcaacctgct gctcgaagaa cagtttgaag acgaagaata acaaacaggg
     2821 cgtacaccaa tacgcccttt ccgtttcaga cggcataccg tgccgcccaa ccgtttcaag
     2881 gaaaaatcat gaaaacagcc cgccgccgtt cccgcgagct tgccgtacaa gccgtttacc
     2941 aatcccttat caaccgcacc gccgcgcccg aaattgctaa aaacatccgc gaaatgcccg
     3001 actttgccaa agcggacgaa gaattgttca acaaactttt cttcggtacg caaaccaacg
     3061 cagcggacta catccaaaaa atccgccccc tgctcgaccg cgacgaaaaa gacctcaatc
     3121 ccatcgaacg cgccgtcctg ctgaccgcct gccacgagct gtccgccatg cccgaaacac
     3181 cctaccccgt catcatcaac gaagccatcg aagttaccaa aaccttcggc ggcacggacg
     3241 ggcacaaatt cgtcaacggc atcctcgaca aactcgccgc ccaaatccgc cccgacgagc
     3301 ccaaacgccg ttgatgcatc acaaatttaa agaaagttct atgtccttat ttctgctttc
     3361 tttgtttcgt cattcccacg caggtgggaa tttggttttt tgaattttgg gtatttcaaa
     3421 taaattctga aacttcaatc tcgtcattcc cgcgcaggcg ggaatccggt tttttgagtt
     3481 gctgccgttt ttgataaact ttggtcgcat cgaatttctg gattcctgcc ttcgtgggaa
     3541 tggcgaaatt tgaaaattgt gggaatttat cggaaataac cgaaacgtaa caaactcaaa
     3601 cgccgttgac gtatcaatca gtcagcaaca caaatgccgt ctgaaaaacc tttcagacgg
     3661 cattttgctt gaccgttcaa tcaatactgt accgtccagc cgatttcgcc gcccgcgcgc
     3721 atcgggacaa gttcgccgtc gccgtaggga acacttgcgg ggacggtttg gctttgtttg
     3781 acgagggtga tcgtgccggc gttttccgga atgccgtaaa accttgcgcc gtttttcgag
     3841 gtaaaggctt cgagtttgtc caacgcgcct gctttttcaa acacttcggc gtaaagctcg
     3901 atggcggtca tcgcgctgaa cataccggcg caaccgcagg cgttttcttt agcggatttg
     3961 gcgtgcggcg cggaatctgt gccgaggaag aatttgtgcg ccttctcgcc ggtaacggcg
     4021 gcaaccaatg cctgacggtg ggtttcgcgt ttgagtacgg gcaggcagaa atgatggggg
     4081 cgcacgccgc cgaccaagag gtcgttgcgg ttgagcaaga ggtgttgcgg ggtaacagtg
     4141 gcggcaacgt tgtcgcctgc ttccaaaacg aggcgggcgg cttcggcggt ggtgatgtgt
     4201 tcgaacacga ctttaagatt tggtacttgc gccaaaacgg gtttcatcac gcgcccgata
     4261 aaggcggctt cacggtcgaa gatgtcgatt tcggggtcag ttacttcgcc gtgaacaagg
     4321 aacaggatgc cctgtttcgc catttcttcc aaaacgggga taagtttgaa caggtcggtt
     4381 acgccggaat cggaattggt ggtcgcgccg gcggggtaga gtttgaaggc aacgatgccg
     4441 gcagctttgg cttcgcgtac aagttcggac gtggcgttat cagtcaaata aagcgtcatc
     4501 aacggctcaa acgcgctgcc ttcgggcaac gccgccataa tgcgcgcttt gtaggcaagc
     4561 gcgtcggcaa cgctgacgac aggcggtttg agattgggca taatcacggc gcgccccatc
     4621 tgtctggcgg tataaggcac aacggctttg agcgcgtcgc cgtcgcgcag gtgcaggtgc
     4681 atatcgtcgg ggcggataat ggtcagggtt tgcatggttt tcctttgttt tatttgaatg
     4741 ttcagacggc atgccgactt tatgccgtct gaagttaggg gagaattagg ggtttcccgt
     4801 tgtgggaacg gtgcgtcagg ttcaaaactg cctgtacggg caaatccggg gaatcggcgg
     4861 tttgtgcggt caatacggtt tcttcggggc tgcccgattc ccgccaggca tagctgattt
     4921 gcctggtttc aaacagtgcc tgcaccgact gttcagccct caattctata ttggtatttt
     4981 tcggcggctg tcggacaagt ccgtcaaaat gttccaaaag cacggcactg tctgcccgct
     5041 cggctttcac actgcaacag tcgcgcggca ggctcaaata ataaatgccg ccatcgcgtt
     5101 caagcgtcca caacgcctgt acatcgggtt ctgcaacagc gggcggcaga cgggacaggt
     5161 cttcctttcc gacagggaca aaatgtccga cagcggcaaa cttggaaact ggttcgaaac
     5221 tgccttgagt caggacgcag gcactccgaa acaaatcgac ggctacacgt gcgaaatcct
     5281 gatgccgtac acaggattgc cggttttggg cattacacgc agcaagcaca aatgccgaca
     5341 gcaataacat caatcgggac atccgcattt cagacggcat ttatttgtgc ttgacgacca
     5401 gcgtgaacac gccgtcctgt tcggaagcat ccaacagcac atgaccggtt tggcggcaaa
     5461 aagcctcaaa atcccccggt gcgccgccgt cggttgccag aacggtcaac acgtcgccct
     5521 gctgcatttg cgccaaagcc tttttcgccc gcaaaatcgg caggggacat ttcaatccgg
     5581 ttacgtctaa agtttcgctg ttcatcgttt gtaccgttta aaaaatggga atcgggggga
     5641 gattataccc cagttgcttg cagccccctt tgccaaccca taaaataacg ccgttttcaa
     5701 tttggagaaa cttatgcgcc gcaccgccct cctgctgctc gcactcaccg ccggcacgtc
     5761 ccttgctgcc ggcttcaacc aaaaagacac gccgatcaac acccgctacc gcgaaacgcc
     5821 cgaagaagcc gccgcccgcg aatttaaaga acataccgcc aaactgccgc cgctgcccga
     5881 tacgcactcg gacggctggt ttgacattta tgtggatgaa aattacggca aacagccgaa
     5941 aatcctgctc gacagcctgc aaattatgcc cgcgcccgac ggcagcatcc gctacattct
     6001 caatatccgc tccgacaaag gctacgacaa cctgactgcc gaaggcatat tctgcgcccg
     6061 cttctccatc aggttcggca acggcaaacc ttcctcctat aaagtgttcg gctacggcga
     6121 cacggtcaac cgccgctgga tacagccccg aaacgccgaa tggaaaccga taggcggtac
     6181 attaagccgc aacgacgcac tgcgcaccgt cctgtatcag gcattctgcg agggcggtgt
     6241 acccgccgac acgaaaggtt tggtacaacg cctgaaagaa cgtgccggac gctatgcccc
     6301 gtcgatgaag ccgcacgaca aataaccaga cacaaaaata ccgtctgaaa catcggtttc
     6361 agacggtatt cgattcatca gccatcaagc ggcggaaact ttgtcctgac ggcacaacag
     6421 cgtaatcaaa tcactgatgc gccgcttcat atcgcggcgg tcgacaatct ggtcgattgc
     6481 gcctttttcc agcaggaact cggcgcgttg gaagctttcc ggcagcgttt cgcgcaccgt
     6541 ctgctcaatc acgcgcggac cggcaaaacc gatcagcgcg ttcggttcgg caagcacgac
     6601 atcgcccaaa aatgcgaagc tggcggatac gccgcccata gtcggatcgg tcaacaccga
     6661 tataaatggc aggcgttttt ccgtcagcaa atgcagcgcg gcactggttt tcgtcatctg
     6721 catcaatgag ttcacaccct cctgcatacg cgcaccgccg gaagccgcca cacagacgaa
     6781 cggacaattg tcggcaaccg cccgacggat accttgtacg aatcgttcgc ccacaaccga
     6841 acccatcgaa ccgccgataa agcggaattc aaacgcggca acgacgacgg gcagaccgtt
     6901 catcttgcct ttcattacca ccaaagcatc atcttccccg gtcagcttgc gtgccgcact
     6961 caaacgatcc ggatattttt tgccgtcttt aaacttcaaa ggatcggtcg gtttgacatt
     7021 tccggcaacc tcctctctgc catcctcatc caaaagcaga ttcaggcgtt gtcgtgccga
     7081 caacggattg tggtggttgc atttcggaca aacctgattg ttctgctgca actcggtcga
     7141 ataaacggtt gccgaacaag acggacattt gtgccacaaa ccctcgggaa cattggaaga
     7201 accgtctttg ctgcggtttt tgattttggg tggcaggatt ttatctaacc agctcatgga
     7261 caactcctaa aatatttaga atgcgtgaca gaatcagcgc acggcatcct tcaactcttt
     7321 gaccaaagca ccgacggccg cagcctcgtt gcccgcattg ttttcaatct ctttcacaat
     7381 ccggctgccg acaatgaccg cgtcggcaac ccggccgatt ttgcgcgcgc tttccgcatt
     7441 gctgatgccg aaaccgacac cgatgggaat atcgatatac tgatgcaaat actctatttt
     7501 acgcgaaacc tcatccgtat ccaaacttgc cgcgcccgtt acgcccttga gcgaaacata
     7561 atagacaaat ccgcctgcca gctcggcaat ggttttaata cggtcttccg tcgtcgtcgg
     7621 cgcaatcagg aaaatacagt cgaccccgtt atccttcagc tcgcgataga gcggatcgat
     7681 ggtttcgaca ggggaatcca ccgtcaacac gccgtccaca cccgccattg cggcttcctg
     7741 agcaaactcc cgataaccca tcttatgtac agggttcaaa tatcccatca aaacaaccgg
     7801 cgtttgcgtg tcggtttcac ggaattttct gacgacatcc aagacatcgc gcagcgaaat
     7861 cccgtttgcc aacgcccgct ccgccgcacg ctgaataacc ggtccatccg ccatcggatc
     7921 ggaaaacggc acgcccaact ccaaaatatc cgcaccgttt gcaaccatgc cgtgcatcaa
     7981 tgccaaagtt gtccgaatat cggggtcgcc caccgtaata tagggaatca atgcctttcc
     8041 gccatccaaa gcggcaaaag cctgccggat tctgctcatt tgtctcttcc atcctatcgg
     8101 caaatatgcc ataaaacaca atcagtaaat ttttcccagt ataacatttg cggtacggat
     8161 aggcgaatac ggcaaacaga aaccacgcaa atcagagccg ccgtttgaac gaggcggaaa
     8221 taaacggcaa gcatctcgga ttatagtgga ttaacaaaaa tcaggacaag gcg
//
LOCUS       DAXHPG010000086         8244 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXHPG010000086 DAXHPG010000000
VERSION     DAXHPG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.086"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(93..653)
                     /gene="sodC"
                     /locus_tag="ACFM6F_001384"
     CDS             complement(93..653)
                     /gene="sodC"
                     /locus_tag="ACFM6F_001384"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFC6164785.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1070..2110)
                     /gene="mutY"
                     /locus_tag="ACFM6F_001385"
     CDS             complement(1070..2110)
                     /gene="mutY"
                     /locus_tag="ACFM6F_001385"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC6164786.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIIRNRNGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2273..3314
                     /locus_tag="ACFM6F_001386"
                     /pseudo
     CDS             2273..3314
                     /locus_tag="ACFM6F_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3337..3555)
                     /locus_tag="ACFM6F_001387"
     CDS             complement(3337..3555)
                     /locus_tag="ACFM6F_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164787.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(3570..4208)
                     /locus_tag="ACFM6F_001388"
     CDS             complement(3570..4208)
                     /locus_tag="ACFM6F_001388"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC6164788.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     RNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4389..6224)
                     /gene="edd"
                     /locus_tag="ACFM6F_001389"
     CDS             complement(4389..6224)
                     /gene="edd"
                     /locus_tag="ACFM6F_001389"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC6164789.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            6740..8185
                     /gene="zwf"
                     /locus_tag="ACFM6F_001390"
     CDS             6740..8185
                     /gene="zwf"
                     /locus_tag="ACFM6F_001390"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC6164790.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT         1927 a         1974 c         2269 g         2074 t
ORIGIN      
        1 cactatactg ctgaagtcgt gaattaaatg ctaaatatct agcaaaaaag tgcggtcaaa
       61 atttaccgca cttttcgttt cgaacaatcg aattatttaa tcacgccaca tgccatacgt
      121 gggccgccac cgccaagtgg agctggatga tcggagtgat tatcaccacc cgtgtggatc
      181 ataatagagt gaccgcgaac atcatctaaa tgtttaagac gtggtgctaa aacaggattt
      241 gttgctgtgc catcatgcaa tacagttaat gcaggtaaat cacctaagtg tgcatcatct
      301 tgccatgggt aaccatgttg ttttgcacct ttaggatccc agtgaccgcc tgcgcctaaa
      361 ccagctgtca atttaccttc tttttctttt ggctcacagc ttgggttttc atggatgtgg
      421 aaaccatgta agccttcgct taatccttgt aaatcagggg taaacacaag accatagtta
      481 gattcagtaa tagtcactgt acccacatct ttgttaccgt ttactggatc aagttgttgc
      541 actttcactt caatagaagc acctttaggt atcgtattat gctcgtgtgc ttgcgcaaca
      601 ccaactgaac atactgcact aaccgctagt gctaataagg ttttcatatt cattgcaatc
      661 tcctttattg cagtaaaaac tcgcgactat tttagtcaat tttgttttaa aagccgataa
      721 atttttacga aaattttaca ttaattgtta tttgcagaaa attagaaccc gttatcacgt
      781 cttggataaa tgccaaaacc attgtgattc agagaattaa aaagaaaaat ttcggaatcc
      841 tcatcctttt ctgaaacctg attgcggtat ccatggcttt gtctttgtca tttaagtttg
      901 tgttcatgtt tttgaccttc tttgcttttt aaatcctttt taaattgccg acttctaccg
      961 ttccaaatgt cccatttgaa cattatcccg ccgcctaaaa tacgacccaa attcatcggt
     1021 tgttcttgct gcgatacctg caaacaacag ttcaatgggt ttattttgtt tattctaacc
     1081 tattaccgtt taaataaatt tccaaaggct tgggcaaacc gtaatctttc aaatgcgccg
     1141 gctttatcca aatgccgtct gaagggcttt cagacggcat ttgtccttca aacggcgtaa
     1201 tcatcagcaa ccggtgcgtc agccggtggg tcagggcggt ttgttcgtcc atatctgcca
     1261 tggtcaggga gaatttggcg gcaaagtcgg aaagcccgtt caaactttca aaacacggca
     1321 cgcaatacag cccgccccaa atgcctttgg cggggcgttt ttccagcaaa atcgcgccgt
     1381 tccggttgcg gataatcagc cagtaaagcg gcagggtcgg tacttcggcg gcggtttttt
     1441 tgcgcggcag ctcggcggtg cggttttgct ttttcgcttc gcagatgtcc gccatcgggc
     1501 attggtggca caagggtttc gtccgtttgc acacggtcgc gcccaaatcc atcaaacctt
     1561 gtgtataggc aggcatatcg gcgttttcag acggcagcag gctttcggca agtgtccaga
     1621 gcgagttttc aaattttttg tcctgcggat tgccgtcgcg ggcgaacacg cggcagagta
     1681 cgcgtttgac gttgccgtcc aaaatggttt cgcggcggtt gaaggagaag gcgcaaatgg
     1741 cggcggcggt gcttctgcct acgccgcaga gggtttccaa gtctttgcgc tccgacggaa
     1801 acgtgccgcc gaattgcctg acgacttgtt gcgcggcttt gtgcaggttg cgcgcgcggc
     1861 tgtaatagcc caagcccgcc cacaacgaca acacttcgtc ttgcggcgcg gcggcaagcg
     1921 tctgaacggt cgggaatttt tctaagaagc gcggatagta gtccaacacg gtggcgactt
     1981 gcgtttgctg gagcatgatt tcggaaagcc agacgcaata agggtttttg acctgccaag
     2041 ggaggtggtg gcgaccgtgt tgtttttgcc agcggatgag ccgttcggag aaggggatgg
     2101 gtgtgttcat taatatcaat cggtggtttt atttatattt aaaacggtat gttattgtat
     2161 aacattgtga aaataattct tattgactta ttttttgtag gggcatataa ctcatataaa
     2221 gaaactttat tgcggtattg aaattattta tcaacaagca aggagtatca gcatgaaagc
     2281 aatggtttat cacggcgcaa acgacatccg ttttgaagaa aaaccccgtc cgcagattat
     2341 cgatccgacc gatgcggtgg tgaaaatcgt caaaaccacg atttgcggta ccgatttggg
     2401 tatttggaaa ggcaaaaaaa cccgaagtcg ccgacggtcg tattctcggt catgaaggca
     2461 tcggtattgt agaagaagtc ggcgatgctg taaaaaacat caaagtcggc gataaagtca
     2521 ttatttcatg cgtcagcaaa tgctgtactt gcgacaactg caaaatccaa ctttattcgc
     2581 attgccgcaa cggcggctgg attttgggct acatgattga cggcacgcag gcagaatacg
     2641 tccgcacgcc ttatgccgac aacagcctcg tgccgctgcc cgacaacgtc aacgaagaag
     2701 tcgccctgtt gttgagcgac gctttgccga ccgcacacga aatcggcgtg caatacggcg
     2761 atgtcaaacc cggcgacacc gtattcatcg caggtgccgc tcctgtcggt atgtccgcct
     2821 tgttgaccgc ccaactgtat agccccgccg ccattatcgt gtgcgatatg gacgaaaacc
     2881 gtttgaaact ggcgaaagag ttgggcgcga cccataccat cagccccgct tccggcgacg
     2941 tctccaaaca agttttcgcc atcgtcggcg aagacggcgt agattgtgcc atcgaagcgg
     3001 tcggtatccc tgcaacttgg aatatgtgcc aagacatcgt gaaacccggc ggccatatcg
     3061 ccgttgtcgg cgtacacggt caatccgttg atttcaaact ggaaaaactg tggattaaaa
     3121 agcttgccat cactaccggc ctggtaaacg ccaataccac cgaaatgctg atgaaggcaa
     3181 tttccagcag ctccgtcgat tacaccaaaa tgttgaccca ccgtttcaaa ttcagcgaat
     3241 tggaaaaagc ctacgacgtg ttcaaacacg ccgccgaaaa tcaagcgatg aaagtggttt
     3301 tggaagcgga ttaatcggcg ttgaatcagt tgttttttaa gcggatgccg tctgaatgcg
     3361 tttcagacgg cattttttca gccgcgtagt atgggctgtc cggctgctgt cgaatgaact
     3421 gaacgttatt tttattatgc ggaaggcgga tgggcatttt aggggcagta tgtcggagtg
     3481 gtgctgccgt gtatgccgac tgcgccgcca agcggcaaaa tgccgtctga gactggaaag
     3541 ggcttcagac ggcattttta cgatgcggtt caggctttgg gtttcaatgc cgccgcttct
     3601 ttggcgagac gggtgatggt gtcccagtct ttgtttttca cggcttcttt cggcgtcagc
     3661 catgagccgc cgacgcacag gacgttgggc agtgccaagt aatcgggcgc ggtggcgagg
     3721 ctgatgccgc ctgtcgggca gaagcgaaca tcggcgtaag ggccgtacag ggctttgagc
     3781 atggctttgc cgccaacgac ttcggcaggg aagagtttga gggtgtcgat gccgtgttcc
     3841 aaagccagtt ggatttcgcc cggggtggca acaccgggaa tcagggggat gccgctgttg
     3901 cggccggctt tggcgaggga ttcatgcagc cccgggctga tggcgaaaac cgcgcctgcg
     3961 tcttcgacgg ctttaagctg ttcgggattg gttaccgtac ctgcgccgac gatggcgttg
     4021 ggcacttctt tggcaatcag gcggatggca tcgaggccga caggggtgcg cagggtgatt
     4081 tcgagggtag ggatgccgcc ttcgacaagg gcgtgggaca aatcgatggc ggtgcttaag
     4141 tcgtcaatcg ccattaccgg cacaactgcg ccggcggtca aaatttcgcg gggggtcagt
     4201 ttggacattt cggttctccg ggtggaatgg ggtattttat taagatggga caggtcgtag
     4261 gtcggattca agaaaccgac atcttggacg gctgtgtcgg attcgagaat ccaacctacg
     4321 tctatcttaa aacacttgaa taatttttca gacggccttc ggtctgaaaa ggccgtctga
     4381 aacgcgcatc aggcaaattc gccgccgaag ctcatcgcgc cggtttcggc gctgctggtc
     4441 atgctgcgga agttggcgaa aagttcgcgg ccgcagcctt gttggttcgc gcccaagtcg
     4501 atgctttcga cttcgcgggc attccattcg gtttcgttaa tcaggacgtt gagttcgccg
     4561 ctaacggagt cgaagcggat caggtcgccg gtacggattt tggcgatgtt gccgcccatc
     4621 agggcttcgg gcgtcatgtg gatggaggcc ggaactttgc cggatgcgcc ggacatacga
     4681 ccgtcggtca gcagtgccac tttgaagccg cggtcttgca ggatgcccaa aggcggggtc
     4741 agtttgtgca attcgggcat accgttggca cgcgggcctt ggtagcggac gacgcacaca
     4801 aaatcgcgtt ccaactcgcc gcgttcaaac gcagccaaca cttcgcgttg gtcgttgaac
     4861 acgatggcag gcgcttcaat aatgcggcag ccttcgcgca cggcggacac tttaatcacg
     4921 ccacgtccga tgttgccttt catcaggcgc agaccgccgt cgggggagaa cgggttgtca
     4981 gctttacgca ggatgtcgtc gttgccgctg gtttcggggg cttcgcgcca ttcgagtttg
     5041 ccgtcgatga ggaaaggctc tttggtgtag tggcgcatac cgtgtccgac gacggtatcg
     5101 acatcgtcgt gcaacaggcc tgcattcagc aattcgcgga taacgaaagg cagtccgccc
     5161 gctgcggtaa agtgattcac gtcggccttg ccgttcggat aaacgcggat gagtagtggg
     5221 atgatggagg aaatttcgtc gaagtcgtcc cagttcaaaa tcacgccggc cgcacgcgcc
     5281 atagcgacga ggtgcatggt gtggttggtc gaaccgccgg ttgccatcag gccaatcaag
     5341 gcgttgataa aggatttttc ggtcaacatt tcgcccaaag gtttaatcgt gccgtttttg
     5401 atgccgcgcg cgaggtgtcc ggcggcgtag cgagtcagcg cttcgcgcag gtcggtgtaa
     5461 gggtggacga aggcggcggc aggcaggtgc acgcccatca tttccatcat catttggttg
     5521 gagtttgccg tgccgtagaa agtgcaggtg cccgggctgt ggtaagaacc catttcgctt
     5581 ttcaaaagtt cgttgcgtcc gaccttgcct tcggcgaaaa gctggcgggt gcgggctttt
     5641 tctttgttgc cgataccgct ggacatcggg cctgcgggga cgaagatacc cggaatatga
     5701 ccgaacgaaa gcgcgcctat catcaaaccc ggaacgattt tgtcgcatac gcccataaac
     5761 aggctgccgt caaacatttg atgcgacagc ccgacggcgg tactcatggc aatcacgtcg
     5821 cgggagaaca gcgacagttc catgccggcg tagccttgcg tgatgccgtc gcacatggcg
     5881 ggcgtgccgc cggcgacttg ggcggtcgcg ccgtttttct gcgcttcgtc tttgatttgg
     5941 tcagggaagt ctttaaacgg ctggtgtgcg gaaaccatgt cgttgtaggc ggtgatgatg
     6001 cctaagttgg ggacggtttc ctgaagcatt tcgattttga tacttttagg catggcagca
     6061 tagccgtgtg ccaagttgct gcagccgagc tggttgcgct ctaagcgtcc catttgtttg
     6121 gcactgcgga tcttcgccag atatttttca cgcgtcggac ggctgcgttc aataatgcgc
     6181 ccggtgattt cggcgagttt ggagtggata ggagtagtgt tcacggtcgg tctcctgtcg
     6241 gaacgggggg tattggggat ttgtttgtgc gtttctgatt tttgtatcgg gtattcagac
     6301 ggtaagtata gtggattaac aaaaaccagt acggcgttgc ctcgccttgc cgtattatct
     6361 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat attttaaaag
     6421 gtatgccgtc tgaaagtggg aagcgggtgt tattatagta caaaatttgt aattttgtta
     6481 caaaaaaatc agacatcgaa aagaaatttg atttaaatcg cagaatcttt cgattgttgt
     6541 aacggaatag gggaaacctg cccgattttt ggactaggtc aaataaattg taaaaatggg
     6601 aatagacaaa aagtgtagcg gtgtgtagta ttactactca ttatgccgcc tgcctttgtt
     6661 tccgatattg cgatgcagcg tgatggggaa aacaggtttc aaacggcatc gaaaaattct
     6721 ttcctgacga gataatacga tgagtacaca aacaaatttt gatttggtgt tgttcggtgc
     6781 gaccggcgat ttggcgatgc gcaagctgtt gccctgtctg taccaggcgc atgttgccgg
     6841 tctgcttcat cccgaagggc gtattttggg ggtcagccgc agcgagttgg ataccgaagg
     6901 ttttctggcg aaggtggaaa ccagttccaa aatccatgtg aaagaaaatt tttccgatga
     6961 ggcttgggca tcgtttgtcg aacgtttcgc atatctcaaa gtcgatgtta cgcagccgga
     7021 cgattttgcc gctttgggcg atttggtcaa agcgcgcaaa gaaaccgaca atgtcgtcat
     7081 ctatctttcc accgcgccga aattcttcgc gcaaacatgt gaaaaccttg ccaaaatcgg
     7141 tttgaacgcc gacaacgtgc gtgtggtttt ggaaaaaccg ttgggtacgg atttggcttc
     7201 ttcccagcaa atcaataccg atgtcgcgcg ttatttcaaa gaagggcaaa tttaccgcat
     7261 cgaccactac cttggcaaag aaagcctgca aaacctgctt gcgctgcgtt ttgccaatgt
     7321 aatgttcgag ccgctgtgga acaataaata catcgaaagc gtgcagttga ccatcgccga
     7381 gcagttgggc gtggaagaac gcggcgagtt ttacgacatt accggcgcgt tgcgcgatat
     7441 ggtgcaaaat cacttgatgc aaatgctgtg catgactgcg atggaagccc ccgccagctt
     7501 ggatgccgac gcggtgcgcg atgaaaaagt caaagtcatc aagtcattga agccgctgac
     7561 cgtcgaatct gtcaatgaaa atgtcgtgcg cggacaatat accgccgcca aaggcatgaa
     7621 cggctatctt gaagaaatca acgttccgca agacagcttt accgaaacct acgtcgccat
     7681 taaagccgaa atcgaaaacg aacgctggaa gggcgttccc ttctacctgc gtaccggcaa
     7741 gcgcatggcg ggcaaagtgg cggaaatcgt tttgaacttc aaagatttga acagccatat
     7801 ttttgaaggc agccgcaccg cgcccaaccg gctcgttatc gagttgcaac catatgaatc
     7861 cgtgcgcctc tatacgcaga tgaaaacccc gggggcagga aataaggtcg aaaccgtgcc
     7921 gctggcaacc gatttgggca aagcattgga aagccgccgc gcggaagctt acgagcgcct
     7981 gctgctggat gtgattaacg gcaaactcgc tttattcaac cgccgcgacg aactcgaagc
     8041 cgcgtgggaa tatgtgatgc cgattttgga aaactggaca aataacacca cgccgccgca
     8101 cggctacggc gcacactcgt gggggcctga agccgcgcgc gaactattgg cgcgcgacgg
     8161 acacaagtgg cacgaagagc agtaatacaa taatgcgttc agacggcatg gggtttgaaa
     8221 tgccgtctga acgtaagtaa agca
//
LOCUS       DAXHPG010000087         8161 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXHPG010000087 DAXHPG010000000
VERSION     DAXHPG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.087"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            73..582
                     /locus_tag="ACFM6F_001391"
     CDS             73..582
                     /locus_tag="ACFM6F_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC6164791.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            925..1365
                     /gene="nadR"
                     /locus_tag="ACFM6F_001392"
     CDS             925..1365
                     /gene="nadR"
                     /locus_tag="ACFM6F_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC6164792.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            1480..1980
                     /gene="hpaC"
                     /locus_tag="ACFM6F_001393"
     CDS             1480..1980
                     /gene="hpaC"
                     /locus_tag="ACFM6F_001393"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC6164793.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            1996..2691
                     /gene="murU"
                     /locus_tag="ACFM6F_001394"
     CDS             1996..2691
                     /gene="murU"
                     /locus_tag="ACFM6F_001394"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC6164794.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEEALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGDAPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLADNPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(2937..3368)
                     /locus_tag="ACFM6F_001395"
     CDS             complement(2937..3368)
                     /locus_tag="ACFM6F_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6164795.1"
                     /translation="MGSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALLLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLNERPSTQEWIGLGLVTAGVLVLALKR"
     gene            3543..5219
                     /locus_tag="ACFM6F_001396"
     CDS             3543..5219
                     /locus_tag="ACFM6F_001396"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC6164796.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLADLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALAKGLPNLLKHISNLKNVFGLPIVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLAHKVVNAIENQPNNFNFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            5333..6424
                     /gene="ychF"
                     /locus_tag="ACFM6F_001397"
     CDS             5333..6424
                     /gene="ychF"
                     /locus_tag="ACFM6F_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC6164797.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LERLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYEDFIALGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            6462..6791
                     /locus_tag="ACFM6F_001398"
     CDS             6462..6791
                     /locus_tag="ACFM6F_001398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164798.1"
                     /translation="MPSEAGFGGFRRFHTAGMRLHQNKIPSAFPICPPRFLQNKPPAP
                     LLQKAVRHSEYPEPRYKMTFQTAFAPPRFEYSGLTLNQDKATKPQTVQIVRQGEATPY
                     WFKFNPL"
     gene            6799..6996
                     /locus_tag="ACFM6F_001399"
     CDS             6799..6996
                     /locus_tag="ACFM6F_001399"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6164799.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTEILLREMSGGGGFP"
     gene            7029..8147
                     /locus_tag="ACFM6F_001400"
     CDS             7029..8147
                     /locus_tag="ACFM6F_001400"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6164800.1"
                     /translation="MPAFFAVLAATLAGGFFLFTKDDFFLLWKSALTALGFASNLYFA
                     RGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVARKSLRVQFGFLAALCALSL
                     AASFIPSALDKYYLPHLRACELLIGSLTAVWMRYRQQRNLAVGKRYAAVGALFSACIL
                     SACLFAYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKFFQWKITVAAGLISYSL
                     YLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYHCIEKPFKKWKGSFAQSVL
                     WIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQPELL
                     VLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEG"
BASE COUNT         1865 a         2141 c         2246 g         1909 t
ORIGIN      
        1 cggaagccaa gttttgagac ttggcttttt gtttgcctgc cggttgcgcg ggcgtaccgt
       61 ttgagagagc gtatgaaaaa gaaactgctt tcgggcatca aatttgccgt tcagacggca
      121 ttggtgtttt tattggtgtc gctgtttttg gattggatac gcaagcccga cgaacctgcc
      181 ggtgcggcag ggcggccttt gaccctgctg tcggggcagc ggctgacttt gggacagttt
      241 agccgggata aggcggtttt ggtgtatttt tgggggagct ggtgcggcgt gtgccgttat
      301 cagtcgccga taatcgatga tttggcggcg gacggcgtgc cggtcgtcgg cgtggcggta
      361 cgttccggca gcgcgtccga agtggcggcg tatatggcaa agcggggctt gggctttccg
      421 acggtcaacg atgaggacgg gggtttggca aggtcttggc ggattgtcgc aacgcctgcc
      481 gtcgttttgg tcaaaaatgg gaaaatggtt cactatacga cgggaattag cagttattgg
      541 ggcttgcgcg cacgaatttt tcaggcggat gttttcggtt aacttttgtt tttgttaaac
      601 tttacgctat gtagagaatc aaacggcata taccttgctc gtctgatgcg gcggcttttg
      661 ttttttctgg aagaaagttc tttcgacagt aaatgtaata ctttgttgag attgtaggga
      721 aaatagacta agatataact attaaaatat ttttagatag gattatcgga attaaagtct
      781 tttataccca gtcgtccgat gcggtttata gcgtattgtt gctatatgtt cgttttgtta
      841 tataacggtt gcatcaaaat ttacgcccac aggctttccc gacggtttga aagtttgatt
      901 ttcgataact tggagacttg aacaatgcct acccaatcaa aacatgcgtc tatcaatatc
      961 agtctgatac aggcaaggga agccctgatg acccaattca ggcctattct gaatcaggcg
     1021 aatattaccg atcagcaatg gcggattatc cgtcttttgg cggaaaacgg cacgctggac
     1081 tttcaagatt tggcgaatca ggcgtgtatt ctgcgcccca gcctgaccgg tatcctgacc
     1141 cgccttgaaa aagcgggttt ggttgtccgc ctgaaacctt ccaacgacca acgccgcgtt
     1201 tttctgaagc tgactgccga gggcgagaag ctgtatgagg aaatcggcga agaagtggac
     1261 gagcgttacg acgctatcga ggaagtgctg ggccgcgaga aaatgctgct gcttaaagac
     1321 ctgttggcag aacttgccaa aatcgaggat gcgttgaact cgtaatacgc cgtaacgcgc
     1381 ggaaatgtcc gaccgacggc tttttgaatc agaactgctg cacatggggg gtgccttgtg
     1441 tgcagcattc ttatataggg gacagtttaa aggggaaaaa tggcggattt gcagaaaact
     1501 tttcaaactt cgttccgtga tgcgatggca tcctgtgcgg caggcgttca tgtcatcacg
     1561 acagacggtg cggcagggcg ttacggcatt acaatgacgg cggtcgcgcc ggtaacggac
     1621 gagccgccga ccgtgatgct gtgcatcaac cggagtgcgc gaatcattcc gatcctgtcg
     1681 gaaaacggca gcctctgcat caatatgctg gcggacgaac atcaggatgt tgccgaacat
     1741 tttgccgggc tgaccggcct gtcgcccgaa gagcggtttg cctaccatat ttggcatcgc
     1801 ggcaaaacgg gacaacttga aatagaaggc gcgttggcgc acctgcacgg gcatattgtc
     1861 ggcaaacatg aaatcggcac gcattttgtg ttttacgtca ggctcgacga aatcaaaaac
     1921 tgcgggtgca aacgccccgc gctgctgtat ttcagacggc agtttagatt tttagactga
     1981 tattcggaca gatatatgaa agcgatgata ctggcggcag gacgcggcga gcgtatgcgc
     2041 cctttgaccg ataccactcc gaagccgctg ctcgatgtgg cgggtaagcc tctaatcggt
     2101 tggcacctat gccgtctgaa gcaggcgggg tttaccgaaa tcgtcatcaa ccatgcttgg
     2161 ttaggtcggc agatagaaga ggctttgggc gacggctcgg cttatggcgt gaacatcgcc
     2221 tattcgcccg aacccgcagg cggcttggaa acggcaggcg gcatcgcgca ggcattgccg
     2281 ctgttgggcg atgccccgtt tttggtcgtc aatggcgacg tgctgaccga catcgatttt
     2341 accgccgcgt ttcaggcggc atcgtccctg cccgagcaca tttccgccca tctgtggctg
     2401 gcggacaatc cgccgcacaa ccccgacggc gatttttccc tgctgcccga cggcagcgtg
     2461 cggccggaag taaatggcgg caacggattg acatttagcg gcgtgggtat ttaccgtcct
     2521 gaaatgtttg atggaatcga agcgggcagt gtggcgaaac tcgcgcccgt attgcggggc
     2581 gaaatgcggc aaaaccgcgt cagcggacag aagcatacgg gcttgtggct ggatgtcggc
     2641 acggtatgcc gtctgaaaga ggctcaagcc cttgcagggg cttggaagtg aaataaaacg
     2701 gtggcttgcc gagccggctg ccgaacggtt ccgaccggat gtccggcata ttttcgtgga
     2761 ggcgtgaaaa tagggcgttc ggggctttcc gtccaacaaa tgccatctga agccgacccc
     2821 tgtcggatgc ggcggtttgc cgtcggcgcg gtgtcggatt ttattgggta actttgttca
     2881 gaccgccgcc cgtcagattg tcaaaacccg gtttcaggcg gtatggcgga ttcggtttaa
     2941 cgtttcaacg ccagcaccaa cacgcccgcc gttaccagcc ccaagcctat ccattcctgc
     3001 gtgctggggc gttcgttgag gaagactact gccatcaggg cgaccagaac caggctgaat
     3061 ttatcgacgg gggcgacttg cgaggcgtta cccagttgca gggctttgaa gtaggcaagc
     3121 caagatgcgc cggtagcaag gccggatagg ataaggaatg tccagttgtg ccccgtaaag
     3181 ccgttcacgc cctgccattt gccggtgtag gttaaaaaca ataacaaagc ggcgaggatg
     3241 accaaggtgc ggatgaaggt agcgaaatcc gaatctatgc cctgcaagcc cattttggcg
     3301 aaaatagcgg tcaatgaggc gaagcctgcc gatgccaatg cccaaaacag ccatgcgttg
     3361 ctgcccatgt tttctccttg gattgtgaac aatatgaacg gtatttttgt tgcagcgtca
     3421 aaaatttcac tgcgggtttg gtgcggataa cgttataata tgcctgatat tattttcaat
     3481 ccacctgttt gtcgcctgat gctttcagac ggcatgtccc tcctcatttc taaaggaaaa
     3541 tcatgagctt caaaaccgat gccgaaatcg cccaatcctc caccatgcgc ccgattggcg
     3601 aaattgccgc caagctgggt ctgaatgccg acaacattga gccttacggt cattacaagg
     3661 cgaaaatcaa tcctgccgaa gcgttcaaac tgccgcaaaa acagggcagg ctgattttgg
     3721 ttaccgccat taatccgact ccggcgggcg aaggcaaaac caccgtaacc atcggtttgg
     3781 cggacgcgtt gcgccacatc ggcaaagatg ccgtgattgc cctgcgcgaa ccttctctgg
     3841 ggccggtgtt cggcgtgaaa ggcggcgcgg caggcggcgg ctatgcccaa gttttgccga
     3901 tggaagacat caatctgcac tttaccggcg acttccacgc catcggtgcg gcgaataacc
     3961 tgctcgccgc catgctcgat aaccatatct accaaggcaa cgagttgaac atcgatccga
     4021 aacgcgtgct gtggcggcgc gtggtcgata tgaacgaccg ccagttgcgc aacatcatcg
     4081 acggcatggg caaacctgtt gacggcgtga tgcgtcctga cggtttcgac attaccgttg
     4141 cttccgaagt gatggcggta ttctgccttg ccaaagacat cagcgatttg aaagagcgtt
     4201 tgggcaacat ccttgtcgcc tacgccaaag acggcagccc cgtttacgcc aaagatttga
     4261 aagcacacgg cgcgatggcg gccctgctca aagatgcgat taagcccaac ttggtacaaa
     4321 ccatcgaagg cactccggcc ttcgtacacg gcggcccgtt cgccaacatc gcccacggct
     4381 gcaactccgt aaccgcaacc cgtctggcga aacaccttgc cgactacgcc gtaaccgaag
     4441 caggcttcgg cgcggatttg ggtgcggaaa aattctgcga tatcaaatgc cgtctggcgg
     4501 atttaaaacc tgatgcggct gttgtcgtgg ctactgtccg cgctttgaaa tacaacggcg
     4561 gcgtggaacg cgccaacctc ggcgaagaaa atttagacgc tttggcaaaa ggtctgccta
     4621 atctgctgaa acacatttcc aacctgaaaa acgtattcgg actgcccatc gtcgttgcgc
     4681 tcaaccgctt cgtgtccgac tccgatgccg agttggcgat gattgaaaaa gcctgtgcgg
     4741 aacacggcgt tgaagtttcc ctgaccgaag tatggggcaa aggcggcgcg ggtggtgcag
     4801 acttggcgca caaagtcgtt aacgccatcg aaaaccaacc gaacaacttc aattttgcct
     4861 acgatgtcga gttgggcatc aaagacaaaa tccgtgcgat tgcccaaaaa gtgtacggcg
     4921 cggaagatgt tgatttcagc gcggaagcgt ctgccgaaat cgcttcactg gaaaaactgg
     4981 gcttggacaa aatgccgatc tgcatggcga aaactcaata ctctttgagt gacaacgcca
     5041 aactgttggg ctgccccgaa ggcttccgca tcgccgtgcg cggcatcacc gtttccgccg
     5101 gcgcgggctt catcgtcgcg ctgtgcggca atatgatgaa gatgccgggt ctgccgaaag
     5161 ttccggccgc cgagaaaatc gatgtggacg aacacggcgt gattcacggc ttgttctgaa
     5221 cggtttttga aaccggatgc cgtctgaagc cgtttcagac ggcatttttt cggaacgcgg
     5281 gcggcggtat gctataatcc gccgttaaat ttctctattt tcaggaaaaa acatgagttt
     5341 gaaatgcggc atcgtcggtt tgcccaacgt cggcaaatcc accctcttta acgcgctgac
     5401 ccaatcgggt atcgaagcgg caaactatcc tttctgtacc atcgaaccca acgtcggcat
     5461 cgtcgaagtg cccgacccgc gtatggctga attggcaaaa atcgtcaatc cgcaaaaaat
     5521 gcagcctgcc atcgtcgaat ttgtcgatat cgccggtttg gttgcaggcg cgagcaaagg
     5581 cgaaggtctg ggcaaccagt tccttgccaa catccgcgag actgacgcga ttgttaacgt
     5641 cgtacgctgc tttgacgacg acaacatcgt ccacgttgca ggccgcgtcg atccgattgc
     5701 cgacatcgaa accatcggta cagagttggc gcttgccgac ttggcaagtg tggaaaaagc
     5761 catcgtccgc gaagaaaaac gcgcccgttc aggcgacaaa gacgcgcaaa agctggtcga
     5821 tttgtgcaaa aaactgctgc cgcatctaga cgaaggcaaa cccgtgcgtt ccttcggttt
     5881 ggacgcggaa gaacgcgcga tgctcaaacc gctgttcctg ctgaccgcca aaccggcgat
     5941 gtatgtgggc aacgtcgccg aagacggttt tgaaaacaat ccgcacctcg aacgcctgaa
     6001 agaattggcg gcaaaagaaa acgcccccgt cgtcgccgtt tgcgccgcaa tggaaagcga
     6061 aatcgccgaa ttggaagacg acgaaaaagc cgagttcctc gccgaaatgg gcttggaaga
     6121 accgggcctg aaccgcctga tccgcgcggg ctacgacctc ttaggcctgc aaacctactt
     6181 caccgccggc gtgaaagaag tccgcgcttg gaccatccac aaaggcgaca ccgccccgca
     6241 agccgccggc gtgattcaca ccgactttga acgtggcttc atccgcgccc aagttatctc
     6301 ctacgaagac ttcatcgctt taggcggcga agccaaagcc aaagaagccg gcaaaatgcg
     6361 tgtggaaggc aaagaatacg tcgtgcagga tggggatgtg atgcactttt tgtttaacgt
     6421 gtaacccaaa tgcggcaggt tttcagacgg ctttgccaga aatgccgtct gaagccggtt
     6481 ttggtggttt tcggcgtttc cataccgccg gaatgcggct gcatcaaaat aaaatcccgt
     6541 ccgcatttcc gatttgccct ccccgattcc tgcaaaacaa accgcctgcc ccgctgttgc
     6601 aaaaagccgt ccggcattcc gaatatcccg agccccgata caaaatgacc tttcagacgg
     6661 catttgcgcc gccgcgtttc gagtatagtg gattaacttt aaatcaggac aaggcgacga
     6721 aaccgcagac agtacagata gtacggcaag gcgaggcaac gccgtactgg tttaaattta
     6781 atccactata aaaacattat gagccaagcc ttaccctacc gcccggacat cgacacattg
     6841 cgcgccgccg ccgttttgtc cgtcatcgtg ttccatatcg aaaaaaattg gctgccgggc
     6901 ggatttctcg gtgtcgatat attctttgtg atttcaggct ttttgatgac ggagatcctc
     6961 cttcgcgaaa tgtcgggggg gggaggtttt ccctgaaggc attttatatc cgccgcatca
     7021 agcggattct gcccgcattt ttcgccgtat tggcggcaac gctggcaggc ggcttctttt
     7081 tattcaccaa agatgatttc tttcttttgt ggaaatccgc gctgaccgcc ttgggtttcg
     7141 cctctaacct gtattttgca agggggaagg attatttcga tcccgcgcag gaagaaaagc
     7201 ccctgctgca catctggtct ttgtcggtcg aagaacaatt ttactttgtc tttccgatac
     7261 tgctgctgct tgtcgcccgc aaaagcctgc gcgtacagtt cggcttcctt gccgcactgt
     7321 gtgccttaag ccttgccgcc tcctttatac cttccgcgct cgataaatat tacctgcccc
     7381 acctgcgcgc ctgcgaatta ctgataggat cgctgaccgc cgtgtggatg cggtaccggc
     7441 aacagcggaa tctcgccgtc gggaaacggt atgccgccgt cggcgcattg ttttccgcgt
     7501 gcatactgtc cgcctgcctg tttgcctatt cggaacaaac cgcctatttc ccaggccccg
     7561 ccgctttgat tccctgtctg gctgttgccg cgctgattta tttcaaccat tacgaacacc
     7621 cgcttaaaaa atttttccaa tggaaaatca ccgttgccgc cggtttgatt tcctattcgc
     7681 tttatctgtg gcattggccg atattggcct ttatgcgcta tatcggcccg gacaacctgc
     7741 cgccttattc gccggcggca gcgatcgtcc tgactctagc gttttccctg atttcttatc
     7801 actgcatcga aaagccgttt aaaaaatgga aaggttcgtt cgcacaatcc gttttatgga
     7861 tttatgcctt gcctatgctc attttggggg cgggctcgtt tttcgcgatg aggctgccgt
     7921 ttatggcgca atacgaccgc ttggggctga cgcgttccaa cacctcctgc cacaacaata
     7981 ccggcaaaca atgcctatgg ggggatacgg aaaaacagcc ggaactgctg gttttgggcg
     8041 attcccacgc cgaccattac aaaacattct tcgatgccgt gggcaaaaaa gaaaaatggt
     8101 ccgccacaat ggtttccgcc gacgcttgcg cctatgtgga aggctgagac ctttgcaaaa
     8161 t
//
LOCUS       DAXHPG010000088         8120 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXHPG010000088 DAXHPG010000000
VERSION     DAXHPG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.088"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(214..2010)
                     /locus_tag="ACFM6F_001401"
     CDS             complement(214..2010)
                     /locus_tag="ACFM6F_001401"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC6164801.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGILSDMVSLTGKRTLAQSLAAKNIRIEHPDGLLDQVWTSRPAIPAETVFVH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPSAEQKSDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2468..3721
                     /locus_tag="ACFM6F_001402"
     CDS             2468..3721
                     /locus_tag="ACFM6F_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6164802.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCIISATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3727..3879
                     /locus_tag="ACFM6F_001403"
     CDS             3727..3879
                     /locus_tag="ACFM6F_001403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164803.1"
                     /translation="MPSETANRFQTALSRLWKKMPTSRKICANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            3876..5387
                     /gene="lysS"
                     /locus_tag="ACFM6F_001404"
     CDS             3876..5387
                     /gene="lysS"
                     /locus_tag="ACFM6F_001404"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC6164804.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5571..5801)
                     /locus_tag="ACFM6F_001405"
                     /pseudo
     CDS             complement(5571..5801)
                     /locus_tag="ACFM6F_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5805..6683)
                     /locus_tag="ACFM6F_001406"
     CDS             complement(5805..6683)
                     /locus_tag="ACFM6F_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6164805.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLLVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEALDLPEGSVILGVVLEVKKRF
                     MSAAQARRRVDSDYRP"
     gene            complement(6713..7840)
                     /gene="fic"
                     /locus_tag="ACFM6F_001407"
     CDS             complement(6713..7840)
                     /gene="fic"
                     /locus_tag="ACFM6F_001407"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6164806.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCITARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVAKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
BASE COUNT         1786 a         2041 c         2255 g         2038 t
ORIGIN      
        1 cgcgccttag ctcaaagaga acgattctct caggtgctga agcaccgagt gaatcggttc
       61 cgtactattc gtactgtctg cggcttcgcc gccttgtcct gatttttgtt aatccactat
      121 atttgcttgc ggcaagatga aaacggtgcg ggatgtttta ggaaaccaat gccgtctgaa
      181 ggctttcaga cggcattttt tgcgccgtgc tgtttaacgc gccagcggtt cggtgcgttt
      241 gatcagccac gcttttgccg cgccttcggt cagcggctcg aggcggcggc ggacttcggc
      301 gtggtagcgg ttgacccagt cgatttcgcc gtcggtcatg agggcggtgt ccatcaggcg
      361 tgtatcgatg gggcagaggg tcagggtttc aaaacagagg aagctgccga attcggtttc
      421 ttgaggggcg gcgacggctt ggttggcggc aaggttttca atgcggatgc cccattttcc
      481 cgggcggtag agtccgggtt cgatggaggt aaccatgcct tttttcatgg cggtttcggg
      541 cgtggcgggg gcggcgaagg cgatgcgctg cgggccttcg tggacgttga ggaaatagcc
      601 tacgccgtgt ccggtgccgt ggccgtagtc gcattgcgcc tgccacaggg gtttgcggca
      661 aatcgcatca atcagcggcg aggggatgtt ttcggggaat acggcttcgg caagcgcgat
      721 atgggctttg agaacgaggg tgttgtcgct tttttgttcg gcactcggcg tgccgacagg
      781 gacgacgcgg gtgatgtcgg tcgtgccgcc tttgtattgc gcgccggagt cgatgagcaa
      841 aagcccgttg ccgctgatgg tgctgtggct ttcgggcgtt gcgctgtaat gcggcagtgc
      901 gccgttggcg ttgaagcctg cgatggtgtc gaaactcaat gaaatgaagc ctgggcgcac
      961 gctgcggtgg cgataaagca tggtgtccac gtcgatttcg gtcaggctgc cgccgttgcc
     1021 gatgatgtct tcaaactcgg cgaagaaacc gcacaacgcc gcgccgtcgt gttccatcgc
     1081 ttcgcggatg cgggcgatgt cggcttcgga tttgcaggat ttgaacagcg tggatgggtt
     1141 gattccctcg ataaggcgca cgctttcggg caggcgcaca agcgtgctga cggcggtttt
     1201 gttcggttcg ataagcagcg cgccgccgat ttgcgcgagt ttgtcggcaa cttgggcata
     1261 aggttcgacc gtgatgcctg cggtttgcag cgcggcggcg gcttcggcgt tcagacggca
     1321 tcggtcggta aacaggacgg cgttgtcttt gccaatcagc aggaaggaca cgaaaacagg
     1381 gttgaaaggt acgtcgctgc cacgcaggtt ggtcagccag gcgatgtcgt cgagcgagga
     1441 aaccaagtgg taatccgcgc ctttttccgc catcacggcg cgcacgcggg cgagtttttc
     1501 ggcggcggtt tcggagacgt aggcggggtc gtggacgaac acggtttcgg caggaatggc
     1561 cgggcggctt gtccacactt ggtcaagcag gccgtcgggg tgttcgatgc ggatgttttt
     1621 ggcggcgagc gattgcgcta aagtgcgttt gccggtgagc gagaccatat cggaaaggat
     1681 gccgacggcg gcgttttcgg gcaggtttgc cgcgagccat tcgttatacg gcggcacttg
     1741 cccgcttttt tgcagttcga tgccgctgcc cgcaagctgc ttggcggctt gttcccaata
     1801 gcggctgtcc acccatacgc ccgcttcgtc ggcggtaacg acgaatgtgc cgaccgagcc
     1861 ggtaaagccc gacaattcgc gccgcgcctg ccaatgttcg ggcaggtatt cggacaggtg
     1921 ggggtcggcg gaagggatga cgagtgcatc caagccttgc gccttcatgg cttcgcgcaa
     1981 tgcggacagg taattcgata cggtattcat aataattcct ccaatcgggt tttgcggctt
     2041 cagacggcat ggacggaaat ctgaaatgcc gtctgaaaaa cataaccatc ttatcaaatc
     2101 gccagcctgc tgcaaaacgg acgggctgat cggatggtgc gtgtttgcgc gaaaaaagcg
     2161 atgtaaggtt gtttgttgta aaattttagg cataaaatgt cgtttcatgc ctttttttgg
     2221 aagtaatacg gaaaatatag tcaagtttga gcatgaagtt ctaatagtgt gtgttggcag
     2281 gtatttggat tacgttacaa tacgcgcttt catcaaaatt tccctaccga gcggtatcct
     2341 gtttttgaag acggtaggat ttgggtttgt ttgggggcaa tcccgcccaa attggagcaa
     2401 tagatagggc tgtgtccgaa tatgcggctc tgtgtgttga aacacggtta aaaatacgga
     2461 agactttatg tccgaacaac atatttcgac ttggaaaagt aaaatcaacg cattggggcc
     2521 ggggatcatg atggcttcgg cggcggtcgg cggttcgcac ctgattgcct cgacgcaggc
     2581 gggcgcgctt tacggctggc agatcgcgct catcatcatc ctgaccaacc tcttcaaata
     2641 cccgtttttc cgcttcagcg cgcattacac gctggacacg ggcaagagcc tgattgaagg
     2701 ttatgccgag aaaagccgtg tttatttgtg ggttttcctg attttgtgca tcatttccgc
     2761 cacgattaac gctggcgcgg tcgccatcgt aaccgccgcc atcgtcaaaa tggcgattcc
     2821 ctcgctgatg tttgatgccg gcacggttgc cgccttgatt atggcatcct gcctgattat
     2881 tttggtgagc ggacgttacc gcgctttgga tcgcgtttcc aaaatcatca tcgttacttt
     2941 gagtatcgcc acgcttgccg ccgccggcat cgctatgtcg cgcggtatgc agatgcagcc
     3001 cgattttatc gagccgacac cgtggacgct tgccggtttg ggcttcctga tcgcgctgat
     3061 gggctggatg cccgcgccga ttgaaatttc cgccatcaat tctttgtggg taaccgaaaa
     3121 acaacgcatc aatccttccg aataccgcga cgggattttt gatttcaacg tcggttatat
     3181 cgccagtgcg gttttggctt tggttttcct tgcactgggt gcgtttgtgc aatacggcaa
     3241 cggcgaagca gtgcagatgg cgggcggcaa atatatcggg caattgatca atatgtacgc
     3301 cgttaccatc ggcggctggt cgcgcccgct ggtggcgttt atcgcgtttg cctgtatgta
     3361 cggcacgacg attaccgttg tggacggcta tgcccgtgcc attgccgaac ccgtgcgcct
     3421 gctgcgcgga aaagacaaaa cgggcaacgc cgaattcttt gcctggaata tttgggtggc
     3481 gggcagcggt ttggcggtga ttttttggtt tgacggcgta atggcgaatc tgctcaaatt
     3541 tgcgatgatt gccgcttttg tgtccgcccc cgtgttcgct tggctgaatt accgtttggt
     3601 caaaggtgat gaaaaacaca aactcacatc aggtatgaat gcccttgcat tggcaggctt
     3661 gatttatctg accggtttta ccgttttgtt cttattgaat ttggcgggaa tgttcaaatg
     3721 ataacgatgc cgtctgaaac cgcaaaccgc tttcagacgg cattgtcgcg tttatggaaa
     3781 aaaatgccga catcgcgtaa aatatgcgca aattttgtga ttcggtcagg tcgtctgaaa
     3841 cagattgcgc ctgatttatt ttttcggaaa accctatgag cgaacaaaac catccgcaaa
     3901 ccgagccgca gttggacgaa aaccaaatca tcgccctgcg ccgcgaaaaa ctgcacaaca
     3961 tccgccaaca gcgcaacgcc tatcccaacg acttcaaacg cgacagcttc gccgccgatt
     4021 tgcacgccca atacggcgaa atcggcaaag aagaactcga tccgcaaggc attcccgtca
     4081 aagtggccgg ccgcatgatg ctgaagcgtc aaatgggcaa ggcgagcttt gccaccattc
     4141 aagacgtgtc cgggcaaatc cagctttatc tgaacaacaa aggcgtgagc caagaagttt
     4201 tggacgactt caaccattgg gatttgggcg acatcgtcgg cgcggaaggc actttgttca
     4261 aaaccaacca cggcgaactg accgtacgcg tgtccgacat ccgcctgttg tccaaatcct
     4321 tgcgtccgct gccggacaaa cacaaaggct tgagcgatca ggaaaccaaa taccgccaac
     4381 gctatgtcga tttgattgcc aacgaagaat cgcgcaatac ctttatcaaa cgcagccaaa
     4441 tcatccaatc cgtgcgtaat tttatggtgg gcgagcatta tctcgaagtc gaaaccccga
     4501 tgatgcaccc gattcccggc ggcgcgacgg caaaaccctt cgttactcat cacaatgcct
     4561 tagatattcc gctttacctg cgtatcgcgc ctgagctgta tttgaaacgc ctggttgtcg
     4621 gcggcttgga acgcgtgttt gaaatcaacc gcagcttccg caacgaaggc atgtccgtgc
     4681 gccacaaccc cgaatttacg atgattgaat tttacgaagc cttctccgac tacgaacgta
     4741 tgatgcagat ggcggaagac atcatccgca acgcatcgcg cacagtaaac ggtacggcaa
     4801 acatcactta caatggcaaa gaagtcgatt tggaaagccc gttcgagcgt ctgaccatcc
     4861 tcgaagccat caaaaaatac aatccgcact acaccgacga gcagttgaac gatgcggaat
     4921 ggctgaaaaa agaaatcgtc aaacacggtg aaagcctgcc gccgtccccg ggcatcggca
     4981 gcctgcaact cgcgctgttt gaaggttgcg ccgagggcaa gctgtggaac ccgactttca
     5041 tcatcgatta cccggtcgaa gtttccccgt tggcgcgcgc ttcggatacc aaacaaggtc
     5101 tgaccgaacg tttcgaattg ttcgttgtcg gccgcgaact ggcaaacggc tattcagaat
     5161 tgaatgaccc tgaagaccaa gccgaacgct tcaaatcgca agtcgcacaa aaagacgcgg
     5221 gcgacgacga agccatgcat tacgatgccg actacatccg cgcgatggag ttcggtctgc
     5281 cgccgacagg cggttgcggc atcggtatcg accgcttagt gatgctgctg accgattcgc
     5341 aaaccatccg cgatgtgatt ctgtttccgc agatgcgccc cgagtaatca taaaaacagt
     5401 tgaaatggca atgccgtctg aacctgattg gattcagacg gcattttgta tggcaggatt
     5461 gatagtgaat taacaaaaat caggacaagg cggcgagccg cagacagtac aaatagtacg
     5521 gaaccaattc acttggtgct tcagcacctt agagaatcgt tctctttgag ctaaggcgag
     5581 ccaacgctgt accggtttaa atttaattca ctatattcgg tttccaactg accgacccat
     5641 tctgacaagg ccgacaggcg ttgttcggat ttcgccacga acgggttttc ggtatcccac
     5701 gcgtagcctg ccaaaatcga agaaagcata cggctgattt ccgggctgcg gtcgggcgcg
     5761 tacacgacgt tttggaagcg gaaatcgtac caaccgtcca cacatcaggg gcggtaatcc
     5821 gaatcaacac gccttcttgc ctgtgcggcg gacataaacc gttttttgac ctccagcacc
     5881 acacctaaaa tcacgctgcc ttcgggcagg tcgagggctt cagacggcct gcccgtctga
     5941 taaatcagca tcctgcccgc aatatcgata acaaggtgcg cgacgaccag ccgcttgtcg
     6001 gacagttcaa tcagtacgat gtcttcgtcc gcagggcgca tacgcggctc gaccaccagc
     6061 aaatccccct tttcgataac cggcgacatg gacgtgtcgg gcatggcaac ggcaaacgtg
     6121 tactgcgagg gggcgaccac cgaagcaaac aagtcgaaac tgcctgtgag tttgcctgta
     6181 cgggcgaaag ccgccgcctg acggtctgac aacagggggc gtggtgtgag cctcatcgac
     6241 agtgctttgc cgtcggcatc gctgtggttg tgcgtatgcg tatgatggtt gttgatggtc
     6301 gtatcgcctt ggacatcgtt aaggactatg ctgtttgaac ctgccacgga tttggtcatc
     6361 tcattgtagg cttgcgtcgt gatgcgctct tgcttggtca tacctgttac agcctgtttc
     6421 agcatttcta acgctatgtg attcttccta agggtcgcta taccggcttt tgcagcttgc
     6481 tcattaccac aagaggggca accccagcta cttcctatga acatattgga gtacctcgaa
     6541 caggtttggt ttccatgttt agggcagttg attacacacg gcttggctgt accctcaaac
     6601 tcgaccaact taatgccacg atgaccgaaa cgttcctcaa cctcagcagc agcttcttca
     6661 aatgtttttg accttcttgc catattgacc tccggattat tgattatttt ggctatgctt
     6721 taacgaggga ttggaatgag gtaaagctgt tgccctctcc ccgcaatagt tccattaggc
     6781 gcggatgaat gaatagtttg tccctgccga tggcgatttc ttgcagcaca cctatgtctg
     6841 aaagctcttt caggtactta gaggccgtct gccgtttggc tatccctgcc gcttctaggt
     6901 tggcaatgcg tgtatatggc tgctcaaaca gaagatttac cagttcgtgc gtgtagattc
     6961 cttgtgcgtg tgtccgtatg tgttgacgtg tctgctcaaa caggcggcgt atcgcatcta
     7021 ttttcgctac cgtccaatcg gcggtgtcgg ctacgccgtc taagatgtag attatccagc
     7081 tttcccagtc ctgccgttcg gttacgccta aaagcaggcg gtaatagtcc gccctgtttt
     7141 cgataatata gcggctcaaa tacaaaatag gcaaatccaa aagccctttt tcaatcaata
     7201 gcaggctgtt caatatgcgc cccgtccgcc cgttgccgtc cgtaaacgga tggatggctt
     7261 caaattggta atgtgccgcc gccatgatga taagcgggtc taaatcgccg ctttcgtgaa
     7321 taaaccgctc ccaatttgcc agcttgccgc gtatggtttc ttctccttcg ggcggggtat
     7381 agacaacatt tccgctgttg cctcctttta gggctgtgcc gcctgttttg cggatggcca
     7441 tttcgtaggg gtgcttgatg gcgttgcaga ccatgatggc ggtttgtgtg cataaagggc
     7501 ggctcgtcag tgattcatag cctgcaaaca gggcggtgcg gtattgcagg gcttctttcg
     7561 tggcagggtc ttgccgttcc gtatccattt gcagggattg aaacagctta tccgttgtgg
     7621 ttacgatgtt ttcaatttcc gaacttgcac gggcttccat aacaggaagg gtgttaatca
     7681 gcatggcttg attcggtatc agttctgccg cctgctttaa acgggcaagg gatgcacggg
     7741 cggttataca acgtttcagg atggttttgc tttcaatatc ctgttttggc ggcaggggtg
     7801 gtaaatcgtt atagggaata tcgggtttcc agttgctcat atttaaaatt tcggaaaatt
     7861 taaagatgtt tccagtatat gtttacgccg tgtatatatc aaggatatat gtttaaaaat
     7921 ttggcttttg taaatatatg ggcggtaaaa ccgcattatt ttatgttgtt acctatttta
     7981 caccttgtct gcacacgatt tccaaacaac ctaaaaagaa acctgccgaa aggcaggttt
     8041 aaagaggtca ttttaaatgt gaactaaagc ttacaccctc gtagggtatc gtgtatatca
     8101 gacgtactta atatacacta
//
LOCUS       DAXHPG010000089         8111 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXHPG010000089 DAXHPG010000000
VERSION     DAXHPG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.089"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..941
                     /gene="repM"
                     /locus_tag="ACFM6F_001408"
     CDS             <1..941
                     /gene="repM"
                     /locus_tag="ACFM6F_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="HFC6164807.1"
                     /translation="NFHTNRNTAYKALKDTCNNLFERQFSFIEKTPKGEKVVRTRWVS
                     QVAYIEQQATVELVFAPNVAPLITMLEKNFTSYELDQVSSLSSKYAVRLYEIIISWRA
                     AGKTPMFSTMELRERLGMMPDEYQKMELFKRKVLDFAVKQINDKTDISITYEQHKEGR
                     KIVGFTFSILHKIGSKDIPLENQSELFAGMTDLEAGTIRVRAEAYIASLIAKGQNVTK
                     AHRLNILKKAVEERWGFEDVAKDNGAKNPENKNAKVVLNEWEKISNGTRFKDKDGTIW
                     VKDSGMLRTEGTNRWIADSQIAKLFPMLTVMVEEGI"
     gene            1038..1454
                     /locus_tag="ACFM6F_001409"
                     /pseudo
     CDS             1038..1454
                     /locus_tag="ACFM6F_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
     gene            complement(1460..1558)
                     /locus_tag="ACFM6F_001410"
                     /pseudo
     CDS             complement(1460..1558)
                     /locus_tag="ACFM6F_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            1651..>8111
                     /locus_tag="ACFM6F_001411"
     CDS             1651..>8111
                     /locus_tag="ACFM6F_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6164808.1"
                     /translation="MNRTLYKVVFNKHRNCMIVVAENAKREGKNTADTQAVGILPNDI
                     AGFAGFIHSISVISFSLSLLLGSALILTSSSANAQGIVADKSAPAQQQPTILQTGNGI
                     PQVNIQTPTSAGVSVNQYAQFDVGNRGAILNNSRSNTQTQLGGWIQGNPWLARGEARV
                     VVNQINSSHSSQMNGYIEVGGRRAEVVIANPAGIAVNGGGFINASRATLTTGQPQYQA
                     GDLSGFKIRQGNVVIAGHGLDARDTDFTQILSHAVKIDGPVWGKDVRVSAGKNDVSAD
                     GSIRSSHSPATNINSDNSLYAIDTGALGGMYAGKITLISTDRDASVRNQGQWFASAGN
                     VAVNAEGKLVNTGMIAATGENHAVSLHARNVHNSGTVASQDDANIHSQTLDNSGTVLS
                     SGQLTVRNLGRLKNHNNGTIQAARLDMSTGGLDNTGNITQTGSQALDLVSAGKFDNSG
                     KIGVSDVPQTGLNPNPSVIPQIPSTATGSGSSTVLASKPGSNNPVSPTAPAKNYAGGR
                     IQTTGAFDNAGSINAGGQIDIAAQNGLENSGSLNAAKLRVSGDSFNNTVKGKLQAHDL
                     AVNTQTAKNSGHLLTLTGKIDNRELHNAGEIAANNLTLIHSGRLSNDKKGNIRAAHLQ
                     LDTAGLHNAGNILADSGTVTTKNNLRNTGKVSVARLNTEGQTLDNTHGHIEAETVNIQ
                     SQQLTNQSGHITATEQLTINSRNVDNQNGKLLSANQAQLAVSDGLYNQHGEIATNRQL
                     SIHDKNQNTLALNNADGTIQSAGNVSLQAKSLANNGTLTAGNKLDIALTDDFVVERDL
                     TAGKQLNLSIKGRLKNTHTLQASHTLKLNAGNIDNQVTGKIIGGEQTDITSEQHVDNR
                     GLINSDGLTHIGAGQTLTNTGTGKIYGNHIALDAQILLNREETTEGSTKAGAIAARKR
                     LDIGAKEIHNQEGALLSSEGIFAVGNRLDEQHHAAGMADTFVNGSAGLEVQGDALMSV
                     RNMQNINNHFKTETYLAKAEKQVRDYTVLGQNTYYQAGKDGLFDNSQGQKDQTTATFH
                     LKNGSRIEANQWHVRDYHIETYKERIIENRPAHITVGGDLTASGQNWLNKDSRIVVGG
                     RIITDDLNQKEITNQSTTGKGRTDAVGTQWDSVTKKGWYSGRKRQRRTERNHTPYHDT
                     QLFTHDFDTPVSVIQQNAASPSFQPAASAIKLIDGVSTAAVNGQRIHTGNVVSLNNAT
                     VTLPNSSLYTTHPDNKGWLVETDPQFADYRRWLGSDYMLQQLQLDTNHLHKRLGDGYY
                     EQKLVNEQIHQLTGYRRLDGYRSDEEQFKALMDNGLTAAKTFGLTPGIALSAEQVARL
                     TSDIVWMENQTVTLSDGSTQTVLVPKVYALARKGDLNTSGGLISAEQVLLKLQNGNLT
                     NSGTIAGRQAVLIQARNINSNGNIQADQIGLKAEKSINIDGGQVQAGRLLTAQAQNIN
                     LNGTTQTSGNERNGNTAIDRMAGINVVGSHTEQVDNRTSDGILSLHAGNDINLNAATV
                     SNQVKGGTTQITAGNNLNLGTIHTEHREAYGTLDDENHRHVRQSTEVGSSIRTQNGAL
                     LRAGNDLKIRQGELEAEEGKTVLAAGRDVTISEGRQITELDTSVSGKSKGILSSTKTQ
                     DRYRFSHDEAVGSNIGGGKMIVAAGQDINVRGSNLISDKGIVLKAGHDIDISTAHNRY
                     TGNEYHESKKSGVMGTGGLGFTIGNRKTTDDTDRTNIVHTGSIIGSLNGDTVTVAGNR
                     YRQTGSTVSSPEGRNTVTAKSIDVEFANNRYATDYAHTQEQKGLTVALNVPVVQAAQN
                     FIQAAQNVGKSKNKRVNAMAAANAAWQSYQAAQQMQQFAPSSSAGQGQNNNQSPSISV
                     SITYGEQKSRNEQKRHYTEAAASQIIGKGQTTLAATGSGEQSNINITGSDVIGHAGTA
                     LIADNHIRLQSAKQDGSEQSKNKSSGWNAGVAVKIGNGIRFGITAGGNIGKGKEQGGN
                     TTHRHTHVGSTAGQTTIRSGGDTTLKGAQLIGKGIQADTRNLHIESVQDTETYQSKQQ
                     NGNVQVTVGYGFSASGSYRQSKVKADHASVTEQSGIYTGEDGYQIKVRDNTDLKGGII
                     TSTRSAESKGKNRFQTATLTHRDIQNHSRYEGRSFGIGGSFDLNGGWDGTVTDKQGRP
                     TDRISPAAGYGSDGDSKNSTTR"
BASE COUNT         2516 a         1996 c         1879 g         1720 t
ORIGIN      
        1 ataatttcca taccaaccgt aatacggcgt ataaggcttt gaaagatacc tgtaacaacc
       61 tttttgaacg ccaattcagt tttatcgaaa aaacaccaaa aggggaaaag gtagtacgga
      121 caaggtgggt atctcaagtc gcctatattg aacaacaggc aacggtagag ttagtttttg
      181 caccaaatgt tgcccctttg attacgatgc tagaaaaaaa cttcacaagc tacgagcttg
      241 atcaggtctc atcgttgagc agtaaatacg cggtgcggct ctacgaaatt attatttcat
      301 ggcgtgcagc cggtaagaca ccgatgttca gtacaatgga gttgcgcgaa cgtttgggta
      361 tgatgcctga cgagtatcaa aaaatggagc tattcaaacg taaggttttg gatttcgccg
      421 tcaagcagat caatgataaa acggatattt ccattaccta cgagcagcat aaagaaggac
      481 gaaaaattgt aggttttaca ttctcaatcc tacataaaat agggtcgaaa gacatccctc
      541 ttgaaaatca atcggaactt tttgccggaa tgactgattt ggaagccgga acgatacggg
      601 tcagggcgga agcatatatc gcttcgctca ttgcaaaagg tcagaacgtg actaaagccc
      661 ataggctgaa tattctgaaa aaagccgtag aggaacgttg gggatttgaa gatgtggcta
      721 aagataatgg ggccaaaaat cctgaaaaca aaaatgcaaa agtcgtttta aacgaatggg
      781 aaaagatttc taatggcaca cgttttaagg acaaggatgg aacaatttgg gttaaagatt
      841 caggtatgct taggactgaa gggacaaaca ggtggatagc cgattctcag attgccaaat
      901 tatttcctat gttaactgtg atggttgagg aaggtattta acccggcaac tcgcgtattc
      961 tacctgtttt gcggtacgga aaccaattga acctgcttta cgctacaata gaagattgca
     1021 attttgtcgg atgtaccatg aacgattaca cagccatgcc gtctgaagac ggaggaatcg
     1081 gctcattatc gcttccgccg cactcaatgg aggcggaaca atccgttttg ggcgggttga
     1141 tgctggaaaa tccggcttgg gacaggattg ccgatgtggt ttcgggagag gatttctacc
     1201 gccacgaaca ccgcctgatt ttccgatcca ttgccaaact gatcaatgag agccgtcctg
     1261 ccgatgtcat cacggttcag gaagatttgc agcggaacga agagctggaa gcggcggggg
     1321 gattcgaata tctgattacg ctggcgcaaa atactccgtc tgccgccaac atccggcgcc
     1381 atgccgaaat cgtgcgcgag cgttccatta tgcgccaact cgccgaagtg ggaacggaaa
     1441 ttgcccgcag cgccataccc aaaatatcgg cagcggaacg ggcggtagct tggagtacga
     1501 aaaattcaag cagtttcttt tgaactctat tgtttaattt acaattggtt atctttattt
     1561 tcgtagcctg gcataactgt taatctacgt cagcccctaa aaattaatct aatttacgat
     1621 tataaaaata ttattaagaa agcccttact atgaaccgca ccctgtacaa agttgtattt
     1681 aacaaacatc gaaactgcat gatagtcgtt gctgaaaatg ccaaacgcga gggcaaaaac
     1741 acagccgaca cccaagctgt aggtattttg ccaaatgata ttgcgggctt tgcgggtttt
     1801 atccattcta tctctgttat ctcattctcc ctttcattac tgctcggttc tgcccttatc
     1861 ctgacttctt cttctgctaa tgcccaaggt atcgttgccg acaaatccgc acctgcacag
     1921 caacagccta ccatcctgca aacaggtaac ggcataccgc aagtcaatat tcaaacccct
     1981 acttcggcag gggtttctgt taatcaatac gcccagtttg atgtgggtaa tcgcggggcg
     2041 attttaaaca acagtcgcag caacacccaa acacagctag gcggttggat tcaaggcaat
     2101 ccttggttgg caaggggcga agcacgtgtg gttgtaaacc aaatcaacag cagccattct
     2161 tcacaaatga atggctatat tgaagtgggc ggacgacgtg cagaagtcgt tattgccaat
     2221 ccggcaggga ttgcagtcaa tggtggtggt tttatcaatg cttcccgtgc cactttgacg
     2281 acaggccaac cgcaatatca agcaggagac cttagcggct ttaagataag gcaaggcaat
     2341 gttgtaatcg ccggacacgg tttggatgcc cgtgataccg atttcacgca gatattgagc
     2401 catgcggtta agattgatgg gcctgtgtgg ggtaaagatg tgcgtgtgtc ggcagggaaa
     2461 aatgatgtct cagctgatgg ctctatccgg tcttcacact cccctgctac gaatataaat
     2521 tccgacaact cactgtatgc catcgataca ggggcattgg gcggtatgta tgccggtaag
     2581 atcaccctga tcagcactga tcgtgatgca tccgtgcgca atcaggggca atggtttgcc
     2641 tcagccggca atgtggcagt gaatgctgag ggtaaactgg tcaacacagg catgattgca
     2701 gcgacgggag aaaatcatgc ggtttcactt catgcccgca atgttcataa tagcggtacg
     2761 gttgcctcac aggatgatgc caatattcac agccagacgc tggacaattc aggtacggtc
     2821 ttatcctcag gtcaattgac tgtccgtaat ttaggccgtc tgaaaaacca taacaacggt
     2881 acgatccagg ctgcacgctt agatatgtca acaggtggtt tggataacac aggtaatatt
     2941 actcaaacag gttcacaagc attggatttg gtatctgcag gcaaattcga taacagtggc
     3001 aagattggtg taagtgacgt tccacagacc ggtttgaatc ccaatccatc agtcatacca
     3061 cagattccga gtactgcaac aggttcaggc agcagcactg tcttggcatc taagcctggt
     3121 tcaaacaatc ccgtttcacc tacagcacct gcaaaaaact acgccggagg acgcattcaa
     3181 acaacaggag catttgacaa tgcaggatca atcaatgcag gtgggcaaat tgacattgcc
     3241 gcccaaaacg gtttggaaaa ttcgggtagt ctgaatgcgg ctaaactacg agtatcaggc
     3301 gattcattta acaatacggt aaaaggcaaa ctccaggcac acgatctggc tgttaacact
     3361 caaactgcta aaaacagcgg tcacttatta actctaaccg gcaagattga caaccgtgaa
     3421 ctgcataatg ccggagaaat tgccgccaac aatctgacac tcattcattc gggccgcttg
     3481 agcaatgata aaaaaggcaa tattcgagct gcacatttac agcttgatac cgccggttta
     3541 cataatgcag gtaacattct tgccgatagt ggaaccgtta ccaccaagaa taatcttcgc
     3601 aatacaggaa aagtttctgt tgcacgacta aataccgaag gtcagactct agataatacg
     3661 cacggacata tagaggctga aacggttaac atccaaagtc agcaactgac taaccaaagt
     3721 ggccatatta ctgctaccga acaactgact atcaatagtc gaaatgtaga caaccaaaac
     3781 ggcaaactcc tatctgcaaa ccaagcacaa ttagctgttt cagacggcct atacaaccaa
     3841 catggtgaaa ttgccaccaa ccggcagttg tctattcacg ataaaaatca aaacactttg
     3901 gcgttaaaca atgcggatgg cacgattcaa tctgccggta atgtatcgct acaagccaaa
     3961 tcactcgcca acaatggcac attaacagcc ggtaacaaac tggatattgc tttgacggac
     4021 gatttcgtcg tagagcgcga cctcactgca ggcaaacaat taaatctaag cataaaaggc
     4081 cgtctgaaaa atacccatac cctacaagca agccatacgc tcaaactcaa tgccggcaat
     4141 atagataacc aagttacagg caaaattatt ggtggagaac aaacggacat cacatccgaa
     4201 cagcatgttg acaacagggg cttgatcaac agcgacggtt tgacccacat cggtgcaggt
     4261 caaaccctga ccaacaccgg gacaggcaaa atctatggca accatattgc cctggacgcg
     4321 caaatactgc ttaaccggga agaaacaacg gaaggcagta ccaaagcggg ggcaatagct
     4381 gcaaggaaac gtttggatat tggagcgaaa gagattcata accaagaagg tgccctacta
     4441 tccagcgaag gtatttttgc cgtaggtaat cgactggatg aacaacatca tgcggcaggc
     4501 atggccgata cctttgttaa tggcagtgcc ggtttggaag tacaaggtga tgcattgatg
     4561 tccgttcgga atatgcagaa tatcaataat cactttaaaa cagagacata cttagccaaa
     4621 gcagaaaagc aagttcgtga ctacaccgta ctggggcaaa atacctacta tcaggcagga
     4681 aaagacggtt tattcgacaa ctcgcaagga caaaaagacc aaactactgc tacgttccat
     4741 ttaaaaaatg gttctcgtat tgaggccaac caatggcatg tccgagacta ccacatcgag
     4801 acttataaag aacgcatcat cgaaaaccgg ccggcacaca ttactgtggg cggtgatttg
     4861 actgcctcag gtcaaaattg gctgaacaaa gacagccgga ttgtagtagg cgggcgtatt
     4921 atcactgatg atttaaacca gaaagaaatt accaatcaaa gtacaacagg caaaggtcgc
     4981 acagatgctg tcggcacaca gtgggattca gttacaaaaa aaggatggta cagcggtaga
     5041 aaaagacaac gccgtactga aagaaaccat actccttacc atgataccca actatttacc
     5101 cacgacttcg acacgcctgt atccgtcatc caacagaatg ccgcctcccc ttcctttcaa
     5161 cccgctgcat ctgcaatcaa actgattgac ggagtatcca cggcagccgt caatggtcag
     5221 cgcatccata ccggtaatgt ggtctcgtta aataacgcta ctgttactct gcctaacagc
     5281 agcctctata ccacccatcc tgacaataaa ggctggttgg ttgaaaccga tcctcaattt
     5341 gcagactacc gccgctggtt gggcagcgac tacatgttgc aacaactgca attggacacc
     5401 aatcatctac acaaacggct aggcgacggc tactacgaac aaaaacttgt taatgaacaa
     5461 atccatcagt taacaggcta ccgccgactc gacggctaca ggagtgatga agaacaattc
     5521 aaagctctga tggacaacgg ccttactgct gccaaaacat tcggtctcac cccaggtatc
     5581 gccttgagtg cagagcaagt tgcccgctta acttcagata tcgtttggat ggaaaatcaa
     5641 accgtcaccc tgtctgacgg ttcgactcaa accgtactgg ttcctaaagt ctatgccctg
     5701 gcgcgcaaag gtgatctcaa tacctccggt ggcctgatta gtgccgaaca agtcttactt
     5761 aaactgcaaa acggcaacct gactaacagc ggtaccattg cggggcgaca ggccgtactc
     5821 atccaggcac ggaatattaa cagcaacggt aacattcaag ccgaccaaat cggcttaaaa
     5881 gctgaaaaaa gtatcaatat cgacggcggg caggtacaag caggcagact gctgactgcc
     5941 caagcgcaaa atatcaacct taacggtaca acccaaactt ccggtaatga acgtaacggc
     6001 aataccgcca tcgatcgtat ggccggcatt aacgtggtcg gaagccatac tgagcaagta
     6061 gataacagaa cttcagacgg catcctatcc ctgcatgccg gcaacgatat caacctcaat
     6121 gcggccaccg tctctaacca agttaaaggc ggcactaccc aaattaccgc cggcaataat
     6181 ctcaacctcg gcaccattca taccgaacat cgcgaagcct atggtacatt agatgacgag
     6241 aaccatcgcc atgtccgcca aagtaccgaa gtcggcagca gtatccgcac gcaaaacggc
     6301 gcactgctta gagccggtaa cgacttaaaa atccgccaag gcgaactgga ggccgaagaa
     6361 ggcaaaaccg tccttgccgc gggacgtgat gtcactatca gcgaaggacg ccaaataacc
     6421 gaactggata cctcggtaag cggaaaaagc aaaggcatcc tttccagtac caaaacacag
     6481 gatcgctacc gcttcagtca tgatgaagca gtcggcagca acatcggcgg cggcaaaatg
     6541 attgttgcag ccgggcagga tatcaatgta cgcggcagca accttatttc tgataagggc
     6601 attgttttaa aagcaggaca cgacatcgat atttctactg cccataatcg ctataccggc
     6661 aatgaatacc acgagagcaa aaaatcaggc gtcatgggta ctggcggatt gggctttact
     6721 atcggtaacc ggaaaactac cgatgacact gatcgtacca atattgtcca tacaggcagc
     6781 attataggca gcctgaatgg agacaccgtt acagttgcag gaaaccgcta ccgacaaacc
     6841 ggcagtaccg tctccagccc cgaggggcgc aataccgtca cagccaaaag catagatgta
     6901 gagttcgcaa acaaccggta tgccactgac tacgcccata cccaggaaca aaaaggcctt
     6961 accgtcgccc tcaatgtgcc cgttgtccaa gctgcacaaa acttcataca agcagcccaa
     7021 aatgtgggca aaagtaaaaa taaacgcgtt aatgccatgg ctgcagccaa tgctgcatgg
     7081 cagagttatc aagcagccca acaaatgcaa caatttgctc caagcagcag tgcgggacaa
     7141 ggtcaaaaca acaatcaaag ccccagtatc agtgtgtcca tcacctacgg cgaacagaaa
     7201 agtcgtaacg agcaaaaaag acattacacc gaagcggcag caagtcaaat tatcggcaaa
     7261 gggcaaacca cacttgcggc aacaggaagt ggggagcagt ccaatatcaa tattacaggt
     7321 tccgatgtca tcggccatgc aggtactgcc ctcattgccg acaaccatat cagactccaa
     7381 tctgccaaac aggacggcag cgagcaaagc aaaaacaaaa gcagtggttg gaatgcaggc
     7441 gtagccgtca aaataggcaa cggcatcagg tttggaatta ccgccggagg aaatatcggt
     7501 aaaggtaaag agcaaggggg aaatactacc caccgccaca cccatgtcgg cagcacagcc
     7561 ggacaaacca ccatccgcag cggcggggat accaccctca aaggtgcgca gctcatcggc
     7621 aaaggcatac aggcagatac gcgcaacctg catatagaaa gtgttcaaga tactgaaacc
     7681 tatcagagca aacagcaaaa cggcaatgtc caagttactg tcggttacgg attcagtgca
     7741 agcggcagtt accgccaaag caaagtcaaa gcagaccatg cctccgtaac cgagcaaagc
     7801 ggtatttata ccggagaaga cggctatcaa atcaaagtca gagacaacac agacctcaag
     7861 ggcggtatca tcacctccac ccgaagtgcc gaaagcaaag ggaaaaaccg ctttcagacg
     7921 gccaccctca cccatcgcga cattcaaaac cacagccgct acgaaggcag aagcttcggc
     7981 ataggcggca gtttcgacct gaacggcggc tgggacggca cggttaccga caaacaaggc
     8041 aggcctaccg acaggataag cccggcagcc ggctacggca gcgacggaga cagcaaaaac
     8101 agcaccaccc g
//
LOCUS       DAXHPG010000090         8073 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXHPG010000090 DAXHPG010000000
VERSION     DAXHPG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.090"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            96..404
                     /locus_tag="ACFM6F_001412"
     CDS             96..404
                     /locus_tag="ACFM6F_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164809.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            486..1517
                     /gene="ruvB"
                     /locus_tag="ACFM6F_001413"
     CDS             486..1517
                     /gene="ruvB"
                     /locus_tag="ACFM6F_001413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC6164810.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1540..2268)
                     /gene="rpe"
                     /locus_tag="ACFM6F_001414"
     CDS             complement(1540..2268)
                     /gene="rpe"
                     /locus_tag="ACFM6F_001414"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC6164811.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            2414..2848
                     /locus_tag="ACFM6F_001415"
     CDS             2414..2848
                     /locus_tag="ACFM6F_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC6164812.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(2861..3724)
                     /locus_tag="ACFM6F_001416"
     CDS             complement(2861..3724)
                     /locus_tag="ACFM6F_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6164813.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLARLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEVHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPEQRAP"
     gene            complement(3732..4349)
                     /locus_tag="ACFM6F_001417"
     CDS             complement(3732..4349)
                     /locus_tag="ACFM6F_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC6164814.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(4512..5126)
                     /locus_tag="ACFM6F_001418"
     CDS             complement(4512..5126)
                     /locus_tag="ACFM6F_001418"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC6164815.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPGDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQIDLQEG"
     gene            5332..7104
                     /locus_tag="ACFM6F_001419"
     CDS             5332..7104
                     /locus_tag="ACFM6F_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HFC6164816.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFSDGISLTGDDRTLLQTLIRQLGVSLASAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENISFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            7101..7757
                     /locus_tag="ACFM6F_001420"
     CDS             7101..7757
                     /locus_tag="ACFM6F_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6164817.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLNMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
BASE COUNT         1980 a         2372 c         1889 g         1832 t
ORIGIN      
        1 agttttgagg acgataccca atccaagctt tgcaacagcc gccgccatat ccgctataat
       61 tcacgcttca gccattccgc ccccgacata aaatcatgac cctgaaaacc gatttattgc
      121 ctaaaatcaa caacgaagat tatcaacgcc tcatcctcaa acacagtgcg gaatttagcg
      181 gtggcgaaat ccgcctattg aacgaaatcc tcgaaaaatt caatttcgac gttgttcagg
      241 cgcaggcatt ggcgcaagcc gtcatgcagc aaatccgctt cgaccccaac gcctaccaca
      301 tcgacagcga cgacgaagac accaccggca tctgccccca ctgcatcaac ccgcctatgc
      361 cgcccctgcg cgactatctc gtttggcgcg aaacccgcgg ataaaacgct tttgaccgtt
      421 atcttttcaa tgccgtctga aacgccgccg accgttcgga cggcataccc gacaaaggga
      481 acactatgct gcaaaccgac aacctgaccg ccgcgcaacc gcaacgcatc gttgccgccc
      541 aaaccgcctc cgcacaggaa gaactgctcg aacgcgccct ccgccccaaa acgctggacg
      601 actacatcgg gcaagacaaa gccaaagaac agcttgccat tttcatccaa gccgccaaaa
      661 aacgcggcga agcactcgac cacgttttgc tcttcggccc gcccggactg ggcaaaacca
      721 cactggcgca catcatcgcc aaagaattgg gcgtaaattt gcgccaaacc agcggccccg
      781 tcctcgaacg cgcaggcgac ctcgccgccc ttttgaccaa ccttgatccg cacgatgtat
      841 tgttcatcga cgaaatccac cgcctcagcc ctgttgtcga agaaatcctc tatcccgcgc
      901 tcgaagacta ccggctcgac attatgatag gcgaaggacc cgccgcccgt tccgtcaaaa
      961 tcgacctgcc gcccttcacg ctcatcggcg cgaccacccg cgccggtatg ctgaccaatc
     1021 cgttgcgcga ccgcttcggc atcgtctccc gccttgagtt ttacgaaaac cgagacctta
     1081 ccaccatcgt cagccgttcg gcacaactgt tgcagctcga tatgtccgaa gaaggcgcgg
     1141 aagaaatcgc caaacgcagc cgcggtacgc cgcgcatcgc caaccgcctg ttgcgacgcg
     1201 tgcgcgattt cgccgacgtg aaaaacaacg gcacaatcga cggcggcatc gccgatgccg
     1261 ctttaagtat gctggacgtg gacgcgcagg ggctggacgt gatggacagg aaatttctcg
     1321 aagccgtttt gcacaaattc ggcggcggcc cggtcggttt ggacaatgtt gccgccgcca
     1381 tcggcgaatc tacagacacc atcgaagacg ttatcgaacc ctaccttatc caacaaggct
     1441 tcctgcaacg caccccgcgc ggcaggatgg cgaccgaacg cgcctacctg catttcgggc
     1501 tgcccgtcga aaaataacgc aatgccgtct gaaacagagc taattttcag acggcatttc
     1561 tatttcaatc attggcgcaa ggttcagcct gccgcttttt tccagttccg ccctcatcgc
     1621 atcaatcacc gccttatagt ctggtttgcc gaaaatcgca gaaccggcaa caaaggtatc
     1681 cgcaccagct cgggcaacgg cggcaatatt gtcggttttg atgccgccgt ccacttcgat
     1741 ggcgatgtgc cgcccgcttt gtgcttcgta ccgatccagc atcgcccgca cccggcggat
     1801 tttttcaagg gtgtgcggga tgaagctttg tccgccgaat ccggggttga ccgacatcag
     1861 caaaaccata tccagcctgt ccaatacgtt ttccaacaga tatacgggcg ttgccggatt
     1921 caacaccagc cccgcctgac agcccatatc acgaatcagg ctcaagctgc ggtcgatatg
     1981 gcggctcgct tcgggatgaa aagtgataat cgatgctcct gctttggcaa acgactgaat
     2041 caggtcgtca acgggttcga ccatcagatg cacatcgatc ggcacgcttg cataaggctt
     2101 caacgccgcg caaaccatag ggccgaaggt caggttcggc acataatggt tgtccatcac
     2161 gtcaaaatgg atcagatctg cacctgccgc aatgacgctt tccacctctt ctccgaggcg
     2221 ggcaaagtct gccgacaaaa tactgggtgc gatacggtag gcggtcatat tttttccttc
     2281 aatgccttta cgggcggact caacgcggga aagccccgaa acagcattat taacaaaagt
     2341 taactgccat aataccatct ttcacacggc aatcaagtat attgtccgaa atatctggat
     2401 taaaggaaaa gccgtgccac tgcctgctcc ctgccgtttt gccaaacctg ccgcctcttt
     2461 tttaagtatg gctttgcttt cctgtcagct ttcccacgcc gccacggctt atatcccccc
     2521 gaacgatttt caaccgaact gcgacatacg ccgactcggg ctgacccaaa gtcagcacaa
     2581 tgagctgcgt aaaatccgca ccgccttcaa aatggcgggc gacagggcgc gtttgaaggt
     2641 tatgcattcc gaacacagcc gccgccggtc tgtcgtcgaa atcatttctt cggatgtttt
     2701 taatcggaac gaggcgcgcg attatgtcga aagccgctat ttgtccggta tggattttgc
     2761 ggtggacgaa ttggaaatcc aacaccgctt cttccatatc ctcacaccgc aacagcagca
     2821 aatgtggctt tcttcctgcc tcaaataatc cccgaaacgc tcacggcgca cgttgctccg
     2881 gcagcctgcc cgcccagtcg caggcaaact gccacgcgga acgtcccgaa cggctgcccc
     2941 gcgtctgcgc ccactgcaat gccgccatct gtgcggtttc atcatacggc acgccgaaat
     3001 cttccagcca attttgcacc gccgccagat aatcgttttg atcgaacgga taaaaactga
     3061 gccacaatcc gaatcggtcg gacagggata ttttttcttc caccgcttct ttctgatgga
     3121 cttccccccg catccccgtc gtaccggcat tctcgtcaaa atattcgggc atcaggtgcc
     3181 gtctgttgga agtcgcgtac accagcacgt tggcgcaacg ttgagacaga ccgccgtcta
     3241 acgcggtttt caatgcctta taggtttcat cgccgctttc aaacgacaaa tcgtcgcaaa
     3301 atacgataaa tttttccgga cgttccttca aaagcgtcaa caggtaaggc aggccgatta
     3361 aatcgctttt atcgacttcg atcaggcgca atcccttatc cgcatattcg tgcagcaggg
     3421 ctttgaccag cgaggatttg cctgttccgc gcgcgccgct catcaatacg ttattcgcgg
     3481 gtctgccggc aatgaattgt tcggtattac gcaccagcaa ttcggtttgc ctgccaactc
     3541 ccgccagcct tgccaagggg aaggtgtgcg gatcgggcaa gtgttccaaa aaaccttttt
     3601 tgcccgcact ctgccagcgg aaggcaagcg cgttccaatc cgtatgcccg ggttcgggcg
     3661 gaagcacggc atccaaacgc cgcaaaacgg cataggcttt atcgagaaat tcgttcaatt
     3721 ccatctctac ctcactttgc atatctttgc gccatcagcc gttcgacggt atcgacgatt
     3781 gcctgcgtat tcggatcgat ttcgatgttg attctgtcgc cgacctttct gctgccgaac
     3841 agcgtccgtt ccaaagtttc gggaatcaaa tggacattga aacggctgtc ttcgactttg
     3901 cctatggtca ggctgcaacc gtccaagccg acgaaccctt tggtcaggat atagggtttg
     3961 agttcgtgcg ggagcgaaaa ccaaaccgtg cggttgtacc cgtcccgttc gatttcgaca
     4021 ataggcacgg ttgccataat gtgtccgctc atgacgtgtc cgccgatttc gtctccgaaa
     4081 cgcgccgccc gttcgatgtt gacgcaatcg ccttccttca gcagccccaa attggttttt
     4141 gccaaagttt ccgccattaa atcgaaacta acgcggtttc cttcgatttc ggtaatcgtc
     4201 aggcagcagc cgttattggc gaccgatgcg ccgcgttgca gattgtccgc cgcctcttgc
     4261 ggaagctcga cgacataagt gtgaaatgcc tccgacgggc ggtggattgc cgtcagtttt
     4321 cccaatcctt gaacaatgcc tgtaaacata atcctgtttc cctgtgtcgg taaaaatggt
     4381 gcaaattgta gcatctcccc gcgaaaaatg ccgtctgaaa tgccttcaga cagcattatg
     4441 cctccgattc gggcaaaaac cgcccggtat ggcttgacct ttcctttcca cgccggtcgg
     4501 cggtcttgcc cttatccctc ctgcaaatcg atttgcgtgt tcaagtcggc aaaatgcccg
     4561 tcaaactcga atctgaccgg ccgcgccctt tgctgctgca accagcttct taatgttttc
     4621 atacccgaaa acagataggg aatcgcgctt tgcagaatct gcgggcggat atacataatg
     4681 ttgtagtgca tcgttatcgg cgtttccaca taatacgcat tgcacaacgg tgtgcgtttc
     4741 gacacggttt caaacctcgc caccaaatcg cccggcagat acggcatatc gcacggcaca
     4801 accaaaagcc agtcggcagt cgccaactgc aaatcgttgg ctgcggtaca caatgccgaa
     4861 agcgggccga aatgctgcca ctgccgtgcg tccggaaaaa tatgcggact tcttcgagca
     4921 tattcttcca aattccggtt ggtgctgatg gcgatatggc tgacctgcgg cctgaccctg
     4981 tcgatgacat gatctatcag tgccttaccc cccaaaagag caagcccctt gtcctcgcct
     5041 cccatacggc tcgcctgacc gtcggccagt atcagggcaa aagttttcat tgcggatgtt
     5101 ctcttggaaa agttcgaggt tttcatgatt gcagtctgcc gttcccaatg actcaaaatg
     5161 ccgtctgaag cagatggcaa ataaattcat attatctgaa ttttatcata acatgattta
     5221 acactgaagc ggtgcggatt aagttttcct taaccaattc tttctgagca gttgtatcta
     5281 atcccaaaga atgatattgt ttgcattatt tagaacaatt tttcgccgag catgatactg
     5341 ccagcccgtt tttcagacgg catcagcctt tccctacgcc tgaaactcct gaccggagtg
     5401 tgggtcgggt tggcggcatt gtctgtcgtt ttgacactgc tgctctcttt gcgtctggaa
     5461 aacgcggcct ccgtcatcga agaggcgggc aacttgagaa tgcaggcata ccgtctggca
     5521 tacatggcgg gtgaaggatc tccccgctcc caaatagaca atcaggttga cgaatttgaa
     5581 aaaagcctga atcgtatttc acaaagtgat gccatccatc cgctgattcc ttcggacacc
     5641 cctcttgctt atgatttgat acaatccatg ctgattatag attggcaggc acacatcctc
     5701 cccccgctcc agtcctaccg gcgaccgact caggtcgatc tctaccgctt tgccggaaac
     5761 atcgaactgt ttttgcaggc attggaaaat gccaacgaaa aaaacacatg gtggctcagg
     5821 cgttttcaat gggcaattat gttgatgacg ctggtgtcgt ctgtactgat gctgttttgg
     5881 caccagattt gggttatccg gccgctgcag gcgttaaggg aaggtgcgga acgcatcgga
     5941 cggaggtgtt tcgatattcc ggttcccgaa ggcggtacgc cggaattcaa acaggtcggg
     6001 cgttgtttca atcaaatggg cggcaggttg aaaattttat atgatgattt ggaaggacaa
     6061 gtcgccgagc agacacgcag tctcgaaaaa caaaatcaaa acctgaccct gctgtaccaa
     6121 actacacggg acctgcacca atcctacata ccgcaacagg ctgcagaaca ttttctaaac
     6181 cgtatcctgc ccgccgtagg agcagattcc ggcagagttt gtttggacgg cggatccgat
     6241 gtttatgttt ccattcatca tgcggattgc ggcacagcag cttcggattt ggggaagtac
     6301 catgaggaaa tcttccccat tgagtaccag aacgaaacat tgggcaggct gttgctcagc
     6361 ttttcagacg gcatttccct caccggagac gaccgcacgc tgcttcaaac cctgattcgg
     6421 caattgggcg tatcgctcgc tagcgcgaag caggaagaag aaaaacgcct gcttgcagta
     6481 ttgcaggaac gcaacctgat tgcgcaagga ttacatgaca gcatcgcaca agcattaacg
     6541 ttcctaaacc tacaggtaca gatgctggaa accgcctttg ccgaaaacaa acgggaggaa
     6601 gccgcagaaa acatcagctt catcaaaaca ggcgtgcagg aatgttatga agatgtccgc
     6661 gaactgctgc tcaacttccg taccaaaatc agcaataaag aatttcccga agccgttgcc
     6721 gacctattcg cccgctttac gcaacaaacc gggataacgg tcgaaaccgc ctgggaaaac
     6781 ggttcgttcc tgccgcctca ggaagcgcag ctccaaatga tttttatcct gcaggaaagc
     6841 ctgtccaaca tccgcaaaca cgcccgcgcc acccatgtaa aattcaccct ttccgaacac
     6901 ggcggacgct ttaccatgac catccaagac aacggacaag gtttcgacac ggagaaaata
     6961 ggagaaccca cgggcagcca tgtcggactg cacatcatgc aggagcgtgc caaacgcatc
     7021 catgccgttt tagaaatccg ttcccaagct caacagggaa ccaccgtctc attgacggtt
     7081 gcatctgaag aaagcttgaa atgactatta aaattattct gatagacgac cataccctct
     7141 tccgcagcgg cattaaagcc cttttgtcgc gccaacacgg ttttgaagtc atcggcgaag
     7201 ccgcagacgg cctctcgggc atcaaaatga ttagtcggct gcaacccgat gtcgtcctgc
     7261 tcgatttgaa tatgcccggc atgaacggac gcgaagcact ctcccaaatc atcagcatca
     7321 atccgcagca ggcagtgatt atgctgaccg tttccgaaga cagcgacgac ttgaccgaat
     7381 gtattcgcat cggcgcgcgc ggttacctgc tgaaaaacat caacgctgac tttctgctcg
     7441 aaagcatacg caaagccgcc gaaggcgata atgtattctc gcccgagatg accgccaaac
     7501 tcgttaaaag cctgatttcc ccccaacctg cccaagggac acaggcactc tcctcactta
     7561 cccctcgcga actggaaatc ttgggctatc tcgccgcagg acacagcaac aaaatcatcg
     7621 cccgccacct cgatcttgcc gaatccaccg tcaaagtcca cgttcaaaac ctgctccgca
     7681 aactcaacct cagcagccgg gtgcaggccg ccgtttacgc catccggcac aacgtccccc
     7741 aacctgtgcc ggaataaggc gggttcagac ggcatattag gggttttaat ccccgtacgg
     7801 tcattcggat aacagaccaa gcatgtaagt ttatgccccc ataagtacgc ttggcatagc
     7861 agtaatattg ttcggtttag tgttttccgt ttgcccctat ctgatactgc aatatcaact
     7921 atgccgtctg aaaacgcatc atcatgatat tttcagacgg cataataaaa agcggaaata
     7981 ctaatgcagg gtaaaatgtt ccattccgaa tcccataaat atacaatggc ttatatcgtt
     8041 taagcatgtg taacccaccc tcatatcaat caa
//
LOCUS       DAXHPG010000091         8059 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXHPG010000091 DAXHPG010000000
VERSION     DAXHPG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.091"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(182..2224)
                     /gene="recG"
                     /locus_tag="ACFM6F_001421"
     CDS             complement(182..2224)
                     /gene="recG"
                     /locus_tag="ACFM6F_001421"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC6164818.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAIGTHALFSDGVTFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDKLPPGRTPIKTRL
                     VNNVRRAEVEGFVLNTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGELNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQAREIAPILIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2310..2753)
                     /gene="secB"
                     /locus_tag="ACFM6F_001422"
     CDS             complement(2310..2753)
                     /gene="secB"
                     /locus_tag="ACFM6F_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC6164819.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2774..3031)
                     /gene="grxC"
                     /locus_tag="ACFM6F_001423"
     CDS             complement(2774..3031)
                     /gene="grxC"
                     /locus_tag="ACFM6F_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC6164820.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHTAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3245..4738
                     /gene="rng"
                     /locus_tag="ACFM6F_001424"
     CDS             3245..4738
                     /gene="rng"
                     /locus_tag="ACFM6F_001424"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC6164821.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4773..6023)
                     /locus_tag="ACFM6F_001425"
     CDS             complement(4773..6023)
                     /locus_tag="ACFM6F_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6164822.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERVRVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(6036..6193)
                     /locus_tag="ACFM6F_001426"
                     /pseudo
     CDS             complement(6036..6193)
                     /locus_tag="ACFM6F_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6416..7729)
                     /locus_tag="ACFM6F_001427"
     CDS             complement(6416..7729)
                     /locus_tag="ACFM6F_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC6164823.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1902 a         2371 c         2094 g         1692 t
ORIGIN      
        1 ccgcctgcgc gggaatgacg gcggagggct tgccgttttc ccgataagtc tctgcggctt
       61 cccattgccg gatttccgcc cacttgggaa taacgagatt taaaatcatg aaaatgtgtc
      121 aaaaataatg caaaatcaga ttccgttgca cttttttcca atatttccgg cgacatttcg
      181 ctcacacgcc caaataacct tccctgccgg aaagccatcg tgccaaatgc gcttcgacga
      241 tttcggggtt ttgttcaatc agtatcgggg cgatttcgcg cgcttgttcc aaaaggtgta
      301 agtcttcttc gagcttggcg aaacgcagca taggcacgcc gctttgacgc gcgccgagaa
      361 attcgccggg gccgcggatg ttgaggtctt ggcgggcgat ttcaaagccg tcggtgtgtt
      421 cgtagatgac tttcagccgc gctttggcga gttcgcccaa gggttcggca aacaggagga
      481 cgcacacgct ttctgccgcg ccgcgcccga cccgaccgcg taattggtgc agctgcgcca
      541 agcccatgcg ctcggcgtgt tcgatgacca tcagggcggc attgggcaca tctacgccga
      601 cttcgataac ggtggtggcg accaagacgt tcagctcccc cgaagaaaac cgtgccatca
      661 cttcggcctt ttcggcggcc ttcatgcgcc cgtgtaccag tccgatattc agctcgggca
      721 atgccgtctg aagccgggcg agggtttcgg cggcggtttg cagttgcagg gtttcgcttt
      781 cttcaatcaa tgggcagacc caatacgcct gccgcccttt tcggcaagta ttgaggacga
      841 agccttcgac ttcggcgcgg cggacgttgt tgacgaggcg cgttttaatc ggggtgcgcc
      901 cgggcggcag tttgtcgatg acggacacgt ccaaatcggc gaaaaaactc atcgcaagcg
      961 tgcgcgggat gggcgtggcg gacatcatca actgatggac ttcgcgccct ttgtttttaa
     1021 gggcgaggcg ttgggcaacg ccgaaacggt gctgttcgtc cacgatggtc aggcccaaat
     1081 tgtgaaacgt cacgccgtct gaaaacaggg cgtgcgtgcc gatggcgatt ttgacgctgc
     1141 cgtcggcgag tttggctttg gcttcgtctt tggctttttt acgcaaactg ccaaaaaggc
     1201 ggacaacttc aatgcccaaa ggttcgagcc attgtttaaa tttaataaaa tgttgttcgg
     1261 caaggatttc agtgggcgcc attacagcca cctgcgcacc ggattcgatg gcggtcagcg
     1321 cggacaaggc ggcgacaatg gttttgccgc tgccgacatc gccctgcagc aggcggtgca
     1381 tcgggtaggt ttgcgccata tcgcggcaga tttcggaaac aactttttct tgcgcatcgg
     1441 tcagggcaaa cggcagggct tggcgcaggg cttgggtcaa tgtgccgtcg ccgcccaatg
     1501 ccgccgccgt gccgccgata cgcttctgtc gcgccaagcg catcgaaagc tgttgcgcca
     1561 aaagttcatc gaatttgagc cgttgccatg caggcagcgt gccgtctgaa agctgatgaa
     1621 tcgtgaaact cggcggcggc gaatgcaaaa gacgcaggct ttcggcgagg tgtggcagct
     1681 tcagacggca cagcagggca tcgggcagcg tgtcgtgcag cggcgtaacg tccaacgccg
     1741 tctgaataat acggcgcaaa gtgggctggt tcaaaccgtt tacggtcggg taaaccggcg
     1801 tgaggctttc cgccaaaccg ccgccctcgg catcgcggat tttgggatga atcatctcgt
     1861 cgccgtaaaa gccgtgtttg atttcgccca cggcgcggat gcgtttgccg accgccgtct
     1921 gtttctgatg gctggcgtaa aagtggatga agcgcagaaa aaggacgctg ccggaaccgt
     1981 cggcgatttg gacaatcaac tgcttgcgcg gtttgaacgt tacttcctga tggataacct
     2041 cgccctcgac ctggcacggc acgccaatcg gcgcgtcctt aatcggcata atgtgcgtct
     2101 cgtcctcgta acgcagcggc aggtgcaaca ccaaatccca cgcggtatgg aggttgagtt
     2161 tgtcgagctt cttggcggaa acatcggtga ttttgagctg tttctgggtt tcgggcgaca
     2221 tcataggcag attcctttga acgcacctat tttatccgaa aacaaaaata ccgtctgaaa
     2281 cggattcaga cggcatcggc aggcaggaat caagccccgg cggcttcggc ttcctgctgt
     2341 tgttggtaaa tcgcctcaaa attaatcggc gcgagcagga cgggcgggaa gccggcacgt
     2401 gttaccgtac cggaaacggc ttcgcgcgcg taagggaaga ggatgttcgg acacgccacg
     2461 cccagcagca gctgcacatc ttcttcgggg atgttttcca aacggaagat gccactttgg
     2521 gttacttcgt tcaaaaacat cgtgcgctcg ttatccagct tggcggtaac ggttacggta
     2581 acgtccacgt tgtagtagcc gtcttccagc ttttggctgc cggtggaaac gcgcatatcc
     2641 acttcaggct cgccctgttc caaaaagatt tgcggcgcgt gcggcacttc caaagacaag
     2701 tctttgacat acagtcgctc gatgctgaat acgggttgca gttcttcgct cattttgttt
     2761 tcctagttgg gggttaaggg ttcagcagtc cgtccagccc gccttcctgc tggaggcggt
     2821 agaggtcggt aaatccgccg acgtgcgttt cgccgatgaa aatctgcggc acgctgcgct
     2881 gtcccgaaag ctgctgcatt tcggcaaagg cttcggggct tgcatcgaca cggatttcgt
     2941 cgatatgtcc gacacctgcc gtgtgcagca gccttttcgc catcgcgcag tagggacaaa
     3001 acggacctgt gtacatggta acggtctgca tattgggttt ccgaaagttt tgcaatgata
     3061 atcaatatag gggcatttcc cctgtttggc aagtgcggaa cagatgcacg ttcaaacggc
     3121 atgtgcggaa tgtgtcaaag tttctttttt aaagtatgat aggcattgtg aaaaatattt
     3181 ttgcacccgc gctgcgcggc ggaacgggtg caaaaatatt tttattacat tttcaggaaa
     3241 aaccatgttg tcaggactcc ccatccccaa agacatcgcg cgcccgcccg aaacgatatt
     3301 ggtcaacatc acgccgcagg aaacgcgcgt agcggtgttg gaggaaaaca atatctgcga
     3361 gctgcacatc gagcgcaaca gcgaacacag cctggtcggc aatatctatc tgggcgtggt
     3421 gcgccgcgtg ctgcccggga tgcagagcgc gtttatcgac atcggcttgg aacgcgcggc
     3481 gtttttacac atcgtcgatg tcctcgaaca acgccgcaac cccgaagaaa cccagcgcat
     3541 cgaacatatg ctgtttgaag ggcagtctgt tttggtgcag gtcatcaaag acccgatcaa
     3601 caccaaaggc gcgcggcttt ccacccaaat ctcgctggcg gggcgtttcc tcgtccatct
     3661 tccgcaagaa gaccacatcg gcgtgtccca acgcatcgaa gacgatgccg aacgcagcag
     3721 cctgcgcgaa cgcctcgaca agctcctgcc ggaaaatgcc tgccggggct acatcatccg
     3781 caccaacgcc gaaaacgcca ccgacgaaca gctccagtcc gacatcgact acctgaccaa
     3841 agtgtgggaa cacatccaag aacaggcgaa aatccggccg ccggaaaccc tgctttatca
     3901 ggatttgccc ttgagtttgc gcgtattgcg cgatatggtc ggctgcgaca cgcaaaaaat
     3961 cctcgtcgat tccaccgtaa accacgggcg catgacgcgt tttgccgaac aatacgtcca
     4021 cggcgcattg ggcaggatag aattgttcaa aggcgaacgc ccgctgtttg aaacccacaa
     4081 cgtcgaacag gaaatcagcc gcgccctgca accgcgcgtc aacctcaact tcggcagcta
     4141 cctgattatc gaatccaccg aagccatgac cacgatagac gtgaacaccg gcggcttcgt
     4201 cggcgcacgc aacttcgacg aaaccatctt ccgcaccaac ctcgaagcct gccacaccat
     4261 cgcccgcgaa ttgaggctac gcaacctcgg cggcatcatc atcatcgact tcatcgatat
     4321 ggcgcaggaa agccaccgcg aagccgtgtt gcaggagctt gccaaagccc tcgccttcga
     4381 ccgtacccgc gttaccctgc acggttttac cagcctgggg ctggtcgagc tgacgcgcaa
     4441 acgctcgcgc gaaaacttaa accaagtcct ctgcgaaccc tgcccttcct gccaaggcag
     4501 aggccgtctg aaaacgccgc aaaccgtgtg ctacgaaatc cagcgcgaaa tcgtccgcga
     4561 agcgcgccgt tacgatgccg aaagtttccg catcctcgcc gcccccaacg tcatcgattt
     4621 gtttttggac gaagaatcgc aatccttggc aatgctgata gatttcatcg gcaaaccgat
     4681 ttctctggcg gtcgaaaccg cttacacgca ggaacaatac gatattgtgc tgatgtaaaa
     4741 aatgccgtct gaagccttca gacggcatct gtctatttca gggtttcctt gtccaacaag
     4801 gcgcgtatca gcagaccgtg tccgaacctg cggctgtcgg tcagttccaa atatccgccg
     4861 tattttttgg ctagcgtgtc ggcaatcgac agccccagcc ccgtcccctg ctgctccgtt
     4921 cccaaaatac ggtaaaacgg atcgaggacg cggacgcgtt cggattccgg aatgccgttc
     4981 ccgttatctt ccacccacac ggcaagatat ttcccttcgt ctgtgaaacc caaatctatc
     5041 ctgccttcgg acggggtata acgtattgcg ttatcaacaa acgttttaac caaggtatag
     5101 atttcagttt catcggcaaa tatttcgaca tcacctccga ccgccacacc aatgtcctga
     5161 cgtttttcca aggcaagcgg catcagttcc tgcaacactt ggcggaaacg gctttgcaga
     5221 ccgaatgtcg ttttcgccaa aggggtttcg tccgactgcg aacgcgccag tgccaaaagc
     5281 tgctcgagca ggtgtttgtt gcgccgtatg ctttgctgca aaacggcaga ctgccgcgcc
     5341 gcatcgggtg gcagagacat attgttgagc cgttccgcct gaagggaaag ggctgtcatc
     5401 ggcgtacgca attcgtgtgc cgcgtcggcg acaaaccgct gacggtggcg gatgtcttca
     5461 tccgcacgtt tcaaaagcaa gttgatggcg gttacgaacc ctctgatttc actgggaata
     5521 ttgtccacac tcaaggcaga caggtcattg attcggcgtt gttcgagact ttgcgacaat
     5581 ttgcggacgg ggcgcatggc tttgtgcgta atccacacgg tcagcagtat catcagcggc
     5641 agtgccgcca gcaggggcaa cacgctttgc cgtgccgcat ccgccgccaa atcttcccgg
     5701 tattcgcttt cctgcatcac ggcaatccgc ccccgctccg tcgtgcggat atagacgcgg
     5761 taataatcgt cgtccccgtc cgcctgaagc gtgtgcagac cgtccgccag atgcgcaggc
     5821 aggctgacaa cagggtcttc ctgctgcggc atctgtacca aaatacgcgt atcgccgtcg
     5881 ccctcgggca aagtttcggg tttggaatcg ggggcgacgt acaatgccgc ctgacggagc
     5941 aggtcgtcct gcaacgcttc cgtttcgtgg aaggtttcgt agtaggaaaa catacctgca
     6001 agcattgcca gcggaacaaa catccaaacc gtccgccccg gcaaacccaa atccagcagc
     6061 atcaagtcat aaggctgggc agcggcagcc gcgcgccgtt tttgacccaa tccaccgcat
     6121 agccgccgtc tttcaaactt gccgacaccg cctccgcaat catcgcatcg tcttccacca
     6181 gcaaaacacg catcaacttt ccctttcaga caaaccgtgc ctattctaac accccaaaat
     6241 tagccgcaat ttagcggcct ttacgcttgc cggtattttt caaaactgca gcacaaaaaa
     6301 accgcgccgg caactgcctt cagacggcat tggggcgcga ttgcaacaca cgggcaggca
     6361 ggcagagcct gcgacagacc acaggaacga ttcgggcttc agacggcttc gccgtttacg
     6421 gcagaggcac gattcctgcc gctatcgaac tggccaatat cgccagcgac aaaccccacg
     6481 cccaaaaaaa cgaataacgg atgtgtttgc ccatcgacaa tttcgccaaa cccaagccca
     6541 tccacaaagc cggcgaaagc ggcgtaacaa aagtgccgac gatactgccg atcaacatcg
     6601 cataacctgc tgcttcgggc gccacgcccg cctgcgaggt aatctgctcc acaatcggaa
     6661 acagtccgaa ataataagcg tccgtactca aaaccaactc aagcggaatg cccaacacac
     6721 cgatggcaat atgcagataa ggcagcagcg cgtctggcag gatatgcaca atgtctttgg
     6781 aaatcgcgtc caacatcccc gcacccttca aaatccccaa aaacgtacct gccgccaaaa
     6841 taatggacgc catcatcacc gcgccgccgg cgtgggcata aatccgctcc atctgttcct
     6901 gcgggctgcg gtaattcaaa agcaacgccg ccgttgcagc cagcataaat acataacccg
     6961 gcgggaagat gcccgaaaaa agcaggctca tcgccgccaa aaacagcagg acattccacc
     7021 aaaacagttt cggacgcgcc aatttttgtt cttcttccga caaaggcacc ggctttatca
     7081 aatccgccac ggcgggcaac gcgcccaact cccggacaat ccgccttttt tcacgcacac
     7141 ccaaaagcag ggacagcgca aggataaaca ctacaccgat aatttgcacc gtcaacaaag
     7201 gtttatacaa ttcgcccaca tctgcgccca acacgcttgc aacccgccca gtcggcccgc
     7261 cccacggcag aaggttaatc aatcccgcac tggaagtcag cagcaaaaac agcaggtaag
     7321 gattcatatg cagacgcttg taaagcggca aaagggcggg gacgaccaat aaaaacgtcg
     7381 tcgcacccgc cccgtccaac tgcgccacca ccgacaccaa gaccgtcccc acactcactg
     7441 ccacgatatt accccgagtc agcttaatca aaccgcctat catcggacgg aacagcccca
     7501 catcgttcat gattccaaaa aacaaaatgg aaaacataaa cataatcaca atctgcatca
     7561 ccgatttggt gccgcccgaa taaaattctt ttaattggga tacatcaaac cccgccagca
     7621 acgccccaaa cagcggcacc aagattaatg cgatgatggg cgacactttt tccgtcagca
     7681 gcaaccatac gatgaccccg ataatcagca gtccgataaa cgtcagcatc atttctcctt
     7741 tattttattt taaacagaaa accgaccgtg caggcaaaac cgcccacaga cgcgggataa
     7801 gccttgcatt ctactttttt attttgaaac aagtcaatcg gtcatttcct cccatttacg
     7861 cctgccgcca ttcctgtatc cgtccgtcat ttcacggcgg caaccgatac ggaacaaccg
     7921 gcaaaccggt atcgggacgg cgcgggggca ttcatcccgg tacgccgatt caaacgaaac
     7981 cgcccctatc attgcggagc gcggggcgtg ccgtacacgt gggattttat agtggattaa
     8041 caaaaatcag gacaaggcg
//
LOCUS       DAXHPG010000092         8042 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXHPG010000092 DAXHPG010000000
VERSION     DAXHPG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.092"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(68..814)
                     /locus_tag="ACFM6F_001428"
     CDS             complement(68..814)
                     /locus_tag="ACFM6F_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6164824.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(798..977)
                     /locus_tag="ACFM6F_001429"
     CDS             complement(798..977)
                     /locus_tag="ACFM6F_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164825.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(993..1664)
                     /locus_tag="ACFM6F_001430"
     CDS             complement(993..1664)
                     /locus_tag="ACFM6F_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC6164826.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFM6F_001431"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFM6F_001431"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC6164827.1"
                     /translation="MTTQDELKRIAADKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFM6F_001432"
     CDS             complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFM6F_001432"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC6164828.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFM6F_001433"
     CDS             complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFM6F_001433"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC6164829.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAISIADTLKRAEGGQISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFM6F_001434"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFM6F_001434"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC6164830.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGEAEEEEPAAKPVASAEMKLGAKLKVIKADEAELAAHE
                     KYLDGLGEACIWRKEAVPSENGGADA"
     gene            complement(4571..5881)
                     /locus_tag="ACFM6F_001435"
     CDS             complement(4571..5881)
                     /locus_tag="ACFM6F_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6164831.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRYKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFM6F_001436"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFM6F_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC6164832.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="ACFM6F_001437"
     CDS             complement(6056..6355)
                     /locus_tag="ACFM6F_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164833.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="ACFM6F_001438"
     CDS             6706..7902
                     /locus_tag="ACFM6F_001438"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6164834.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAAVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         1910 a         2406 c         1951 g         1775 t
ORIGIN      
        1 ataaatttac cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatacggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctaa ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa accttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaaga aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccataaaccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgga cgctgacttt cctgccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacaaggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatatcgga atcggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tatgccgttt tcgagcacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggctg atcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaagag
     1981 agcgcgcgct ttcgacgact tccacaggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag acgaattttt cggatgcgct ggcgacgatt ttttcgttga
     2101 ggtgtgcgcc gccgccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 caatatagac cgccaacccc gatacttcgt tcagggaaac gacgggaata tcatattggg
     2221 caagcagttc actggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgaaccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttgtcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatcctt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacagcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcattgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggcggat gcccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagccccg cgcggaaaag
     3181 ctgcggcgtt tgtgcctgcc aaaggctcgt ccgctcgaca gtggcactga tttgtccgcc
     3241 ttctgcgcgt ttgagcgtat cggcaatgga aattgccaaa atcccgcctt cggcggcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcagc gcgcggcatc
     3361 gtgtaccaga atattgtcat ccgccgatgc caagccgatt tccaacagtt ttgccacacc
     3421 gtttcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gccccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tcgagatatt tctcgtgcgc cgccagttct
     3781 gcttcatccg ccttaatcac tttcagtttg gcaccgagtt tcatttcggc agatgcaaca
     3841 ggtttggctg ccggctcttc ctcctcggcc tcccccatca ggtcgaactg ccgccgcgtc
     3901 atcgctaaat aaacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac agggcatcca ggctggcttt ctgcccaggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcgacagtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctctatc gtcagaccgt gcaccctcgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcta aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata caggccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaatc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc agcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgctg ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac aggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaggcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcaggc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacaggg caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccaaatg gatttgggta aaggtcggca gttgcgtagt atagaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atagcggtaa agacgggttt gtaccgcacc gtttcacgaa
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacagacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacaggtgct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgcgcctc tgactcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat acccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgttagaat aacctgcttc ttttatcaac cgtttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc catcaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaataaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aagcccgctt cggcaacgct
     7801 gccgtgatta ccactgccga cagcaaagcc gttgccgtgg tcattccgac taacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggtctgt aaggttttat ccgcacacga
     7921 actgcctccg gaaatggagg cagttttttt atccggcttt ccatgcttaa acagcactgc
     7981 ctcttttcag acattgacgg ttgcagccgc ttacctgaac cttatagtgg attaaattta
     8041 aa
//
LOCUS       DAXHPG010000093         8022 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXHPG010000093 DAXHPG010000000
VERSION     DAXHPG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.093"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            110..1051
                     /gene="miaA"
                     /locus_tag="ACFM6F_001439"
     CDS             110..1051
                     /gene="miaA"
                     /locus_tag="ACFM6F_001439"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC6164835.1"
                     /translation="MPTPKAFALLGPTACGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSHAERSFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSPTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRLYPSLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVTDPFSDGNSCTRLIEAAKRFFGA"
     gene            1055..1774
                     /gene="tadA"
                     /locus_tag="ACFM6F_001440"
     CDS             1055..1774
                     /gene="tadA"
                     /locus_tag="ACFM6F_001440"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC6164836.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGAVQTFLLLKASGLT
                     LTKSTLWQLQSLLDGTPPQEMAQHDKTRLLAELKNHPPVAAFPPQGEMAHFMGLALEQ
                     AKLSALMGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1779..2267
                     /locus_tag="ACFM6F_001441"
     CDS             1779..2267
                     /locus_tag="ACFM6F_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC6164837.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLIVGTTFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2333..3085
                     /gene="rlmB"
                     /locus_tag="ACFM6F_001442"
     CDS             2333..3085
                     /gene="rlmB"
                     /locus_tag="ACFM6F_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC6164838.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(3147..4538)
                     /locus_tag="ACFM6F_001443"
     CDS             complement(3147..4538)
                     /locus_tag="ACFM6F_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFC6164839.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(4553..4807)
                     /locus_tag="ACFM6F_001444"
     CDS             complement(4553..4807)
                     /locus_tag="ACFM6F_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164840.1"
                     /translation="MLPIVAYFTAGVKSGGSGMHGAAWRRMRRVCIFVFEIRLLRFAN
                     DIPAATVRNLFAGRARMPSEIEIEAVWRIRYNPRFGFFNI"
     gene            4845..5720
                     /gene="dapA"
                     /locus_tag="ACFM6F_001445"
     CDS             4845..5720
                     /gene="dapA"
                     /locus_tag="ACFM6F_001445"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC6164841.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMTNDTILRLSEIPNIVGV
                     KEASGNVGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            5729..6925
                     /gene="bamC"
                     /locus_tag="ACFM6F_001446"
     CDS             5729..6925
                     /gene="bamC"
                     /locus_tag="ACFM6F_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC6164842.1"
                     /translation="MPSEAFRRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSHAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVLGDYGRNWRRTVLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(6982..7914)
                     /gene="pip"
                     /locus_tag="ACFM6F_001447"
     CDS             complement(6982..7914)
                     /gene="pip"
                     /locus_tag="ACFM6F_001447"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC6164843.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIRKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT         2044 a         2621 c         1884 g         1473 t
ORIGIN      
        1 aaggcatacg gtaaacacgc ccccttgccg tcggcgtgct tccgcaaacc aaaatataac
       61 gtcggcacgc tttccgctat aatccgtatt tttccaaacc tgaaacgtta tgccgacccc
      121 gaaagccttt gccctgctcg ggccgaccgc ctgcggcaaa accgcgctcg ccctcaaaat
      181 tgccgaaacc ttgcctgtcg aaatcatcag cctcgattcc gcgctggttt accgcgatat
      241 ggacatcggc acggcgaaac cgtctcatgc cgagcgttcc tttgtaccgc accaccttat
      301 cgacatcatt acgcctgttc agacatacag tgccgcccgt tttgtcgaag actgcacgcg
      361 tctgattggc gaaatcacgg cgcgtgggaa atatccgctg attgtcggcg gcacgatgat
      421 gtatttccgc gccctgaccc aaggtttgaa cgatttgccc gaagccgacg cctgcctgcg
      481 tgccgacttg gacgaacaaa aacaaatgta cggcttggat ttcctctacc gcaccctgca
      541 aaaagtcgac cctgaaacag catgccgtct gaaaccgaac gacagccagc gtatcggacg
      601 cgctttggaa gtttattatt taaccggcaa accgatgagt acgcatctcg gcagcccaac
      661 ctcccatacg ctccccttcg atttgcatac cgccgccctg attcccgaaa accgcgcccg
      721 cctgcatgaa aacatcgccc tgcgtttcca cctgatgctt gaacagggct ttatcggcga
      781 agtagaaaac ctgcgccgcc tctatcccag cctgaccgcc gactcccctg ccatacgctg
      841 cgtcggctac cgtcaggcat gggaatatct ggacggaaaa accgatttcc cggcatttgt
      901 cgagaaaggc attgccgcca cgcgccaact tgccaaacgc caactgactt ggttgcgcaa
      961 aacaccttta gactgcgtta ccgacccgtt ttcagacggc aattcatgca cacgcctgat
     1021 tgaggcggca aaacggtttt tcggcgcata aactatgctg accacgccgc cgctcgcccc
     1081 caaaaccgtc gccgccctac acaggctcgg tatccgtacg ctcgaagaac tgcggcaaaa
     1141 cggcgcggtt caaaccttct tgttattgaa ggcatcaggt ttgaccctca cgaaaagcac
     1201 gctgtggcag cttcagtcgc tgctggacgg cacgccgccg caagaaatgg cgcaacacga
     1261 taaaacccgc ctgcttgccg agttgaaaaa ccatccgccc gtcgccgcct ttcccccgca
     1321 aggcgaaatg gcgcatttca tgggtttggc attggaacag gcgaaactgt ccgcattaat
     1381 gggcgaaata cccgtcgggg cagtcatcgt ttcagacggc aaaatcatcg catcggcaca
     1441 caacacctgc attgccgact gcaatgtcag ccggcacgcc gaaatcaacg ccttggcaca
     1501 ggcaggcagc gaaatgcaaa actaccgcct tgacggatgc gacatctaca tcaccctcga
     1561 accatgcgcc atgtgcgcgt ccgcactgat acaggcacga atcaggcgcg tgatctacgg
     1621 tgcggcagaa cccaaaaccg gcgcggcagg aagcatcgtc aacctgtttg ccgacaaacg
     1681 cctcaatacg cacaccgcca tacggggcgg aatcctgcaa gaagaatgcc gcgccgtatt
     1741 aagccgtttt ttccaaaaca aaagaaaagg ttgaacctat gaccgccttg ctcgtcatcc
     1801 tcgccctcgc cctgatagcc gccggtacgg cgggcatcgt ttaccccgcc ctgcccggat
     1861 tggcattgat gtttgccgga acctggctgc tcgcctactc cggcggctac caaatctacg
     1921 gcgcgggcgt tttgtggacg gtcggactca tcagccttgc cggcatactg gcggactatg
     1981 tggcaggcat atgggggaca aaatataccg gagcgggcaa gctcgccgtt cgcggcgcat
     2041 tggccggcag catcatcggc atatttttct cccttcccgg actaatactc ggtcccttta
     2101 tcggcgcggc ggcaggcgaa ctgatcgatc ggcgcaatat gcttcaggca ggtaaagcgg
     2161 gcttgggtac gctgttgggg cttatcgtcg gtacgacatt caaaatcggc tgcgccgtat
     2221 ccatcttgtt tatcctgttg gtgaaataca tcgcctacct gttttaaccc cttcagacgg
     2281 cataccggca aattgctata atgccgtctg aacccttttc acaaagcacc atatggcaaa
     2341 ccaaagacct atctacggct tccatgccgt caacgcccga ttgtggcaaa accccaaatc
     2401 catcgtcgaa ctctatgtcc aagaaggcaa aaacgatgta cgcacgcgcg aagtgttgga
     2461 aaaggcggca aacgaaaaca tccgcgtata ttttgccgat gccgaccgcc tcaacgccat
     2521 cagcaaaggt gcgcgccatc agggcgtggt cggatttatc gatgcctcca aaaaccacgt
     2581 ccacctcgaa gacgtattgg aaaacttaag cgaaccgccg ctgctgctga tactcgacgg
     2641 catcaccgat ccgcacaacc tcggcgcgtg cctgcgtacc gccgacgcaa tgggcgtaca
     2701 tgccgtcatc gcaccgaaag acaaaagcgc ggggctgaac gccaccgtca gcaaagtcgc
     2761 cagcggcgcg gcggaaaccg tcccctacat caccgtaacc aacctcgccc gcaccctgcg
     2821 cgagctgaaa gaatacggca tttggatcat cggcaccgat atgggcggca acgccgacct
     2881 ttaccattgc gatctgcccg acagcgcggc atgggtgatg ggcaacgaag gcgacggtat
     2941 gcgccgcctg acacgcgaac attgcgacat gctggtgtcc atacccatgt tcggcacggt
     3001 cgaaagcatg aacgtctccg tgtctgccgg aatggtatta agcgaaaccc gccggcagcg
     3061 cgtattaaaa aacgaaaaag cgtaagcctc aaaaacaatg ccgtctgaaa cagggattct
     3121 gtttcagacg gcattcgttt tcaaaatcag tgttccaagt ggtcggtatc aaacttgacc
     3181 gccgcctcgg ttttatcttc gggcaagacc aaattcagca agactgccgt aatgccgccg
     3241 gcggaaatag agttttggaa caagacaggc aggtttttaa acacttccgg ctcaaacgcc
     3301 acgcccaagc ccaagccgac cgaagtcgcc gcaatcaccg cctcgcgcct gcggatgccg
     3361 tggctgacca aaatccgcac acccgcaatc gcaatcaagc cgaacatcaa aaccatcgcg
     3421 ccgcctaaaa ccggactcgg aatcgtcgta aacgcgcgtc cgacaacggg gaacagaccc
     3481 aacagcacca aaatcacggc aatatatttg cccacatggc gcgaagccac gccggtcatc
     3541 tgaatcacgc cgttgttttg cgcaaacgtt gtcagcggca gcgaacccaa agccgtcgca
     3601 atcaccgaca ccaagccgtc tgccaacacg ccgccgcgca ggcgtttggt gtattcctcg
     3661 ccttcaatcg gctgatcgga caccattgcc gttgcggtca aatcgccgac cgcctcaaac
     3721 acgctcaaca ggaaaatcgc acccgccaca ataaatgcgt gccagtcaaa agcaaaacca
     3781 tatttaaacg gtacgggcag cgtaaccagc ggcaggtttt gcagtgccga aaaatccacc
     3841 ttgcccaaaa acagcgcgac gatatagccg gcaatcagac cgaccgcaat gccgctcatc
     3901 cgcagcagcg ggtttttcat gcagttgaac accagcacaa tcagcagcac cagcgatgcc
     3961 agccccaagt tttccatcga gccgaacgtg ccgtccgcct ttgcgccgaa gccgccgccg
     4021 aaatcggtaa taccgacgtg taccaaactc aagccgatca gcatcaccac cacaccgctg
     4081 accgtcggcg taatcacttt tttcaaatac ggcagaagcc acgccgagaa acacaccaaa
     4141 aacgcgccga caaacgatac gcccaagagc gtcgaaatca tcgcatcctt agtcaaaccg
     4201 ccctctttca tccccgcgcc cagcgcaatc atgacggtaa cgaacgagaa attcaccgac
     4261 tggatagaca gcatccccga accgaccggc ccgaagcggt tgacctgcaa ataagtgccg
     4321 acacccgacg caaccatcgc catcgacacg agatacgccg tcatctccac cggcagttcc
     4381 agcgcgccgc ccacaatcag cgcgggcgta atcatcggca cgaaaatcgc caaaagatgg
     4441 gtaaccgcgc tcaacagcgc attaccgaac ggcggcctat cctccaaacc gtacaccaaa
     4501 tcaggcgcat ccgccccttt tcccatatga ccgctcataa tgtttccttg cttcaaatgt
     4561 tgaaaaaacc aaagcgcgga ttgtagcgaa tccgccatac cgcttcaatt tcgatttcgg
     4621 acggcatacg cgcccgtccg gcaaacagat tccgaaccgt cgccgccgga atatcgtttg
     4681 caaaacgcaa taacctgatt tcaaacacaa aaatacaaac ccgacgcatc cgccgccaag
     4741 ccgccccgtg cataccggac ccgccggatt tcacacctgc ggtaaagtag gctacaatcg
     4801 gaagcattac cgccccctac accacaactg ataaaaggtt ctgtatgtta caaggtagcc
     4861 tggttgccct gattaccccg atgaatcaag acggcagcat ccattacgaa caactccgcg
     4921 acttaatcga ctggcacatt gaaaacggca cggacggcat cgtcgccgtc ggcacgacag
     4981 gcgaatccgc caccctctcc gtcgaagaac acaccgccgt tatcgaagcc gtcgtcaaac
     5041 acgttgccaa acgcgtcccc gtcatcgccg gcacaggggc gaacaacacc gtcgaagcca
     5101 tcgccctttc ccaagccgcc gaaaaagccg gcgcggacta caccctctcc gtcgtcccct
     5161 attacaacaa accttcgcaa gaaggcatgt accgccattt caaagccgtt gccgaagccg
     5221 ccgcgattcc gatgatactc tacaacgttc ccggccgtac cgtcgtcagc atgactaacg
     5281 acaccatcct gcgcctttca gaaatcccca atatcgtggg cgttaaagaa gccagcggca
     5341 acgtcggcag caacatcgaa ctcatcaacc gcgcccccga aggcttcgtc gtcctgtccg
     5401 gcgacgacca caccgcccta ccgttcatgc tctgcggcgg acacggcgtg attaccgtag
     5461 ccgccaacgc cgcgcccaag ctctttgccg atatgtgccg cgccgcgctt caaggcgata
     5521 tcgcccttgc acgcgaactc aacgaccggc tgattccgat ttacgacacc atgttctgcg
     5581 aacccagccc cgccgcaccg aaatgggcgg tatccgccct gggcagatgc gaaccgcacg
     5641 tccgcctgcc gctcgttccg ctgacggaag gcggacaggc aaaagtccgc gccgccctga
     5701 aagcctcagg acaactttaa atatagccat gccgtctgaa gcgttcagac ggcataacgc
     5761 aacaaacact ttaatatcca tcacacagga tgacacgatg acccatatca aacccgtcat
     5821 tgccgcgctc gcactcatcg ggcttgccgc ctgctccggc agcaaaaccg aacagcccaa
     5881 gctcgactac caaagccggt cgcaccgcct gatcaaactc gaagtcccac ctgatttgaa
     5941 caaccccgac caaggcaacc tctaccgcct gcctgccggt tcgggcgccg tccgcgccag
     6001 cgatttggaa aaacgccgca cacccgccgt ccaacagcct gccgatgccg aagtattgaa
     6061 aagcgtcaaa ggtgtccgcc tcgagcgcga cggcagccaa cgctggctcg ttgtcgacgg
     6121 caagtctcat gccgaaatct ggccgctcct gaaagccttt tggcaggaaa acggcttcga
     6181 catcaaatcc gaagaacccg ccatcggaca aatggaaacc gagtgggcgg aaaaccgtgc
     6241 caaaatcccc caagacagct tgcgccgcct attcgacaca gtcggtttgg gcggcatcta
     6301 ctccaccggc gagcgcgaca aattcatcgt ccgtatcgaa cagggcaaaa acggcgtttc
     6361 cgacatcttc ttcgcccaca aagccatgaa agaagtgtac ggcggcaaag acaaagacac
     6421 gaccgtatgg cagccctccc cgtccgatcc caacctcgaa gccgctttcc tgacgcgctt
     6481 tatgcaatat ttgggcgttg acggacagca ggcggaaaac gcatcggcaa aaaaaccgac
     6541 ccttcccgcc gccaacgaaa tggcgcgtat cgaaggcaaa agcctgattg tcttaggcga
     6601 ctacggcaga aactggcggc gcaccgtgct cgccctcgac cgcatcgggc tgaccgtcgt
     6661 cggtcaaaac accgaacgcc acgctttcct ggttcaaaaa gccccgaacg aaagcaatgc
     6721 agttaccgaa caaaaacccg gcctgttcaa acgcctgctg ggcaaaggca aagcggagaa
     6781 acctgccgaa cagccggaac tgattgtcta tgccgagcct gtcgccaacg gctcgcgcat
     6841 cgtcctgctc aacaaagacg gcagcgcata tgccggcaaa gacgcatccg cattattggg
     6901 caaactccat tccgaactgc gttaaaccat tcaaaacaaa agcagcttcc aaacagaagc
     6961 tgctttttta tgcggaactt tttacaacaa atggggcaaa atatcctcaa ctgcctgaac
     7021 caacgcatcc accaaaggcg gatcgaacgc acgatgccct gcctgaacca ccctcaattc
     7081 cgcttcggga aaggctttcg acagcgccca cgcactctgc atcggcgtac acaaatcata
     7141 ccgcccctgc acgataatgg tcgggatatg ccgtattttg ccgatattgt tcaaaatcgc
     7201 cctatcgccc tgcaaccagc cgccgttgac aaaataatgg ttttccaaac gcgcgattgc
     7261 cagtgaggca taagcatctt catccacttc ctccggctcg aaacggatca gatagctttc
     7321 ccaatccgcc caagcctttg cggcagacag gcacacttct tcatcttgat gaaacagcaa
     7381 tccgtgatac gcctcaatca gccggttccg ccgattttca gcaatcggcg cgacaaattt
     7441 ttgccattgt tccggataaa tccggctcac accgcccgcc tcgttcagcc acgccgtttc
     7501 agacggcctg cacaaaaata tcccgcgcaa taccaatccc tttacccgtt caggatgggt
     7561 ttgggcataa gccagcgaca aagtgctgcc ccacgaaccg ccgaacacca gccatttcct
     7621 gatacccagc atttcacgga ctttttcaat atccgccacc aaatcccaag tcgtattgtc
     7681 ttccgcacaa gcatacgggc gcgaacgtcc gcaaccgcgc tggtcgatga tgacgatgcg
     7741 gaacacatcg ggattgaaaa aaccccgaca ttcaggcgaa gcccccgcgc ccggcccgcc
     7801 atgtaaaaaa ataaccggca caccgtcggg attgcccgat tcctcccaat aaatttgatg
     7861 aatttcagac acctgcaagt atccgctatg aaaaggctgt tttatctcat acatcatctt
     7921 ctcccatatt gcttctgaca atccgaacat tcccacacgc aacatcacgc acgagacaca
     7981 agccgccccg aaaggcaaat gccgaaaaat cgcgatatag tg
//
LOCUS       DAXHPG010000094         7949 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXHPG010000094 DAXHPG010000000
VERSION     DAXHPG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.094"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(65..1312)
                     /gene="fabF"
                     /locus_tag="ACFM6F_001448"
     CDS             complement(65..1312)
                     /gene="fabF"
                     /locus_tag="ACFM6F_001448"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC6164844.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLTGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPGIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSLRMIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="ACFM6F_001449"
     CDS             complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="ACFM6F_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC6164845.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            1936..2943
                     /locus_tag="ACFM6F_001450"
     CDS             1936..2943
                     /locus_tag="ACFM6F_001450"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC6164846.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGRPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            2959..3138
                     /locus_tag="ACFM6F_001451"
     CDS             2959..3138
                     /locus_tag="ACFM6F_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164847.1"
                     /translation="MPSERTFCRSDGIFISFFRLPPLENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            3276..3599
                     /locus_tag="ACFM6F_001452"
     CDS             3276..3599
                     /locus_tag="ACFM6F_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164848.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            3686..3967
                     /locus_tag="ACFM6F_001453"
     CDS             3686..3967
                     /locus_tag="ACFM6F_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164849.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            4020..4493
                     /locus_tag="ACFM6F_001454"
     CDS             4020..4493
                     /locus_tag="ACFM6F_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC6164850.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            4507..4842
                     /locus_tag="ACFM6F_001455"
     CDS             4507..4842
                     /locus_tag="ACFM6F_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164851.1"
                     /translation="MPSEALSGLQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WNGVAKAAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKSAAY
                     AILKERGEV"
     gene            5040..7730
                     /gene="glnE"
                     /locus_tag="ACFM6F_001456"
     CDS             5040..7730
                     /gene="glnE"
                     /locus_tag="ACFM6F_001456"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC6164852.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFTYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYSAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDQQTQTLPTSPEQRQLLAESMGFDSYSAFSDGL
                     NIHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQEKPDEEERLGRLKEYGFDAETIATR
                     LDQIRNGHKYRHLSAHAQPRFDAIVPLFVQAAAEQNNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAIVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSTATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWRDVFGEEAATA"
BASE COUNT         1969 a         2033 c         2106 g         1841 t
ORIGIN      
        1 ctataaaaat gccgtctgaa acggtttcag acggcatttc gatgtcggcg gcggctttgc
       61 ggaatcagcc tttgaagcgt ttgaagacca gcgtgccgtt ggtgccgccg aagccgaagg
      121 agttggaaat ggcaacgtcg atttccgcgt cgcgcgcttc gttggcgcag tagtccaaat
      181 cgcagccggc ttcaacgtct tgttcaaaaa tgttgatggt cggcgggatt ttgccgtcgt
      241 gtatcgccaa aatgctgtac acggcctcca cgccgcccgc cgcgccgagc aggtggccgg
      301 tcatggattt ggtcgagctg acgacggttt tgtaggcgtg ttcgccgaac gcgcgtttga
      361 gggctttggt ttcgttggca tcgcccaagg gggtggacgt gccgtgcgcg ttgacgtaat
      421 ccacgtcttc gggattgatg ccggcatctt tcagcgcgcg ggtaacggca agggcggggc
      481 cttcttcgtt cggcgcggtg atatggtaag catcggaact catgccgaag ccgacgattt
      541 cggcgtagat tttcgcgccg cgttttttgg cgtgttccaa ttcttccaac accaatatgc
      601 ccgcgccttc gccgataacg aagccgtcgc ggcctttgtc ccacggacgg gaagcggtgg
      661 cggggtcgtc gttgcgggtg gagagggctt tcatcgcggc aaaaccgccc acgcccaaag
      721 tgctgattgc gccttccgcg ccgccggcaa ccattatgtc cgcgtcgccg tatttaatca
      781 tacggaggga atcgccgatg gcgtgcgcgc cggtggtgca ggcggaaacc atcccgtagc
      841 tcgggccgcg gtagcctttg aggatggtaa cgtgtccgga aatcagatta atcagagaac
      901 cggggataaa gaaagggttg attttgcgcg cgccgccttc gattacggct ttgccggtga
      961 cctcgatgcc gggcagtccg ccgatgccgg aaccgatgtt cacgccgatg cggtctttgt
     1021 cgaggttttc cacatcgtcc aaacccgaat cggcgattgc ctgcaatgcg gcggcaatgc
     1081 cgtagtggat gaatacgtcc atccggcgcg cttctttcgc gctgatgtat tgtccgatgt
     1141 cgaaaccgcg cacctcgccg gcgacacggc tgttgatgtc ggatgcgtca aagcgggtaa
     1201 tcgcgccgat gccgcttttg ccggtgagca gggtgtccca agcctctgcg acagtgttgc
     1261 cgacagggga aacctgacct aagcctgtaa tgactactct tctctgactc atgataacct
     1321 cgctgttggt tgtcggaatg ggggcatatg cggctgtcgt gcagatgccg tctgtaattt
     1381 gcggcagggg ttcaaacagt ttgccatata agggaaaagc ctctattgcg cggtgcagca
     1441 gaggctgttg tgtcgggcga cgaccggtta gccgttgtgg gcattgatgt agtcgatagc
     1501 cagttggacg gtggtgattt tttcggcatc ttcgtcgggg atttcgcagc cgaatgcttc
     1561 ttccaaagcc ataaccagct ccacggtgtc caaagaatcc gcgcccaagt cgtcttggaa
     1621 agaagattcg tttttcacgt cggcttcgtt tacgcccagt tgttcagcaa caattttttt
     1681 aacttgttgt tcgatgtttg acatatcagt cgttccttta tgccttgcgg caggttgttt
     1741 aagggaaata aatcggtggt attgtaccga cttttaatag agttttctat ctaatgacta
     1801 ttatatcaat atttgccgat ttgtacattt ttgggtgcgg cgggttttgt cgttcaagtt
     1861 tgacctgtgt gccgtatgtt tgacgggatt tcggttaaaa tgacggcatt tccatctgaa
     1921 gcagaaagcc ctgtcatgta tccacttgcc cgccgcatcc tgtttgcact cgatgccgag
     1981 aaagcccacc acttcacgct cgacgcgctc tacacggttt ataaattggg tctgattccc
     2041 gtaaccggca accgtaccaa acctgtaaaa ttgatgggta tggatttgcc caaccctgtc
     2101 ggacttgccg ctggactcga caaaaacggc gaatacatcg acgcattggg cgcgctcggc
     2161 tttggtttca tcgaaatcgg cacggtaacg cccaaaccgc agcccggcaa cccgcagccg
     2221 cgcctcttcc gcgttcccga acaccaaggc atcatcaacc gcatgggttt caacaaccac
     2281 ggtatcgaca ccatgatacg caacatcgaa aaaagtaaat tcagtggcgt attgggcatc
     2341 aacatcggta aaaacgcggt tacacccatc gaaaacgctg ccgatgatta tttaatctgc
     2401 cttgaaaaag cctacgcaca cgcaagttac attaccgtca acatttcctc gcccaacacc
     2461 aaaaacctcc gcgcgctgca aggtggcgac gagttgagcg cattgcttga ggctttgaaa
     2521 aacaaacagg cacagcttgc ctctgtatac gggaaatacg ttccgctcgc cgtcaaaatc
     2581 gcccccgatt tggatgaagc acaaatcgaa gacatcgccc acgttgtcaa atccgtcgaa
     2641 atggacggca tcatcgctac caataccacc atcgacaaat caagtctcgg cagccatccg
     2701 ctcgcaggcg agcagggcgg tttgagcggg cggcccgttc atgaaaaaag taatcgggtg
     2761 ttgaagctgt tggcagacca catagacggc aagctgccga ttatcggcgt aggcggcatt
     2821 atggaaggca gggacgcggc agataaaatc cgcttgggcg caactgccgt ccaggtgtac
     2881 agcggattga tatacaaagg tccggcattg gtcaaagaat gtttgaaggc tttggcgcga
     2941 tgacgcgatc cgcccaaaat gccgtctgaa cgcacgtttt gccgttcaga cggcattttc
     3001 atttcctttt tccgcctgcc gccccttgaa aatcccttac gcgccgccct gtttgaaaca
     3061 aagcaaacgc ccgccgcgaa ccggacaatc ttgatacgca gccaaagcaa gtccggcggt
     3121 aagttgttaa atatataagc aattgataaa aaaaataatt ttatgccgtg gtggacggtt
     3181 tgagaaaacg caaaatatga acggcgttga aattcatgat gtggaaaata aggatacggc
     3241 ggataatcaa aagattttat atttaaggat ttgatatgtt taaggacgaa ctaaatgaat
     3301 ttatccgatt gatatccgat ccggaaagtg aattggatga atggtatttg agtgatttta
     3361 aagatgaaca tatatgggaa atgcaaagtt acgaagcatt ttcatgtttg cgggaagcag
     3421 tcccctattt gtttgcttat ccccgatacg ggtatgaact gttggagata atttccgcgt
     3481 taaaagaaac atctgatacg acagaattat tttatgagcc gggtatcgtg cctttgctga
     3541 ttgatttata taaagaagat agttatctgg taaatatggt caaacggata ttcaaatgat
     3601 gaaaggggcg cgggcgtttg cttggcggaa ggcaggtaac tcaatttgaa ggacgcggaa
     3661 gccgatacgt taaaaaggat gaaaaatgga aaacgggcaa cggaataacc ggtttccttt
     3721 ggaaaaacga attttttatc tggagcattc ggggcggtat ctgatgattt gcgccttatc
     3781 ggattattcc caaaacaaac atactgtcgt tatggcaaat ttcatctatc cggatgaaaa
     3841 aacggattgg cggaatttgg atgatttatt caatgagttg gttttagagg aattgcaggc
     3901 ttcatttatg gattggcatc cgactgttga agaggcaatc agccgtcatt tggaagactt
     3961 ttcgtaacag gcgtcaatcg gaaggggcgg aatgacgggg caggcaatcg gagtaaaaaa
     4021 tgaaccttga tttaaccgcg caaaaagtcc gtctttcttg gaaggatatt ctgtgggggt
     4081 atgggaataa atacttgggt tgggctgatg tggcagctta tgcccgaaaa atgacgcttt
     4141 cagatcatga tgaacgtgtg ttcaaactat ctttaatcaa caaatccaat attcttgaat
     4201 taaagcctgt tctggaagat ttggcttcgg aaatgaggga ttattcccct aaaaattggc
     4261 tgtacgtcct cttaagcgat gtattccata gaaaagaaga atttgaggat cctttggggg
     4321 aagttgaaaa aatttatgca gattttgatt atccggaaga aatagaatca tttgtcaggt
     4381 atatgccgcc caaagacggt tatattcctt ctgcccacac ctatgaagaa aatattgccc
     4441 ggttatattc tcactgggaa cactatttga acaacggcgg agggcagggt taaaaccggc
     4501 aatccgatgc cgtctgaagc attatccggc cttcagacgg cattttgttt tccgacagtt
     4561 tataaactgt cgttgtttct tgacagaaac aacgacctta tttatttgaa acgattggag
     4621 gacatgatta tgggtttttg gaatggtgtg gcaaaagcag caaaagcagt gggagaggga
     4681 atgattgaag ccggcaatga gcataaggcg ttgaaaatgg aatatgcgga gaaatcaagt
     4741 gaggagctgc atgaaatcgt caagagtgat ggttttttta aaaattccac acgggagaaa
     4801 agtgcggctt atgctatttt aaaagagcgt ggcgaggtgt gaacaggaaa cggcggcatt
     4861 tgccgctgtt ttttatcggc aggcatccgt ctggatgtcg gggcaaggtt tcagacggca
     4921 tcgaaggttg ctatgatata gtggcttgac tttaaaccgg tacggcatcc cctcgccctg
     4981 tcctgatttg aagtgaatcc gccatcatct tcctgccatt ttttcaaacg gaatccgaaa
     5041 tgtccgacaa ccgcctcgac accgcccgcc gccattccct cttcctcgcc cgccagctcg
     5101 acaacggcaa actcaagccc gaaattttcc tgcctatgct ggacaaggtt ttgaccgaag
     5161 cggatttcca agcctttgcc gactgggaca aaatccgtgc ggaagaaaac gaggaagaat
     5221 tggcgcggca gttgcgcggg ttgcgccgtt atgtggtgtc gcagattatc gtgcgcgata
     5281 tcaaccgcat cagcgatttg aacgaagtaa cccgcacgat tacgctgttt gccgattttg
     5341 ccgtcaatac cgcgctggat tttgcctacg cctattatcg ggacatgtac ggcacgccga
     5401 tcgggcgtta taccaaatcg ccgcagcatt tgagcgtggt ggcgatgggc aaggcgggcg
     5461 gctatgagtt gaacgtgtct tccgacatcg atttgatttt cacctatccc gaatcgggcg
     5521 acaccgacgg caggcgcgaa cggggcaatc aagagttttt caccaaagtc gggcagaaac
     5581 tgattgcgct gctgaacgac atcaccgccg acggacaggt gttccgcgtc gatatgcggc
     5641 tgcggccgga cggtgattcg ggcgcgttgg tattgagcga aaccgcgctg gagcaatatt
     5701 tgattacgca ggggcgcgaa tgggaacgct acgcgtggtg caaaggccgc gtggttacgc
     5761 cgtatccgaa cggaatcaaa tcgctggtgc gccctttcgt gttccgcaaa tatttggatt
     5821 acagcgcgta tgaggcgatg cgtaagctgc accgccaaat cagcagcgaa gtcagcaaaa
     5881 aaggcatggc ggacaacatc aaactcggcg cgggcggcat ccgcgaagtc gaatttatcg
     5941 cccagatttt ccagatgata cgcggcggac aaatgcgcgc gctgcaactg aaaggcacgc
     6001 aggaaacgct gaagaagctt gccgaaaccg ggatcatgcc gtctgaaaac gtcgaaaccc
     6061 tgcttgccgc ctaccgcttc ctgcgcgatg ttgaacaccg cctgcaatac tgggatgacc
     6121 agcaaaccca aaccctgccg acctcgcccg aacagcggca actgctcgcc gaaagcatgg
     6181 gtttcgacag ttattccgct ttttcagacg gtctcaacat tcatcggaac aaagtcaatc
     6241 agttgttcaa cgaaattttg agcgaacccg aagagcaaac gcaagacaac agcgaatggc
     6301 aatgggcatg gcaggaaaag cctgatgaag aagaacggct aggccgtctg aaagaatatg
     6361 ggtttgatgc cgaaaccatc gccacgcggc tcgaccaaat ccgcaacggc cataaatacc
     6421 gccacctttc cgcccacgcc cagccgcgtt ttgacgccat tgtgccgttg ttcgtacaag
     6481 cggcggcaga gcaaaacaac ccgaccgata cattgatgcg gctgttggat tttctcgaaa
     6541 acatcagccg ccgatccgcc tatctcgcct tcctcaacga acatccgcaa accttggcgc
     6601 aactggcgca gattatgggc caaagttctt gggtggcggc gtatctgaac aaatatccga
     6661 ttttgttgga cgaactcatc agcgcgcagc ttttggatac cgcgtttgat tggcaggcgc
     6721 tcgccgccgc cctttcagac gacctcaaag cctgcggcgg cgatactgaa gcgcaaatgg
     6781 acaccctgcg ccatttccaa cacgcccaag tcttccgtct cgccgtccaa gacctcgccg
     6841 gactgtggac ggtagaatcc ctctccgacc aactctccgc cctcgccgac accatcctcg
     6901 ccgccgccct gctgtgcgca tgggcggaca tgccaaaaaa acaccgcgac acaccgcaat
     6961 tcgccatcgt cggctacggc aaactcggcg gcaaagaact cggctacgcc tccgacctcg
     7021 acttggtcta tctttatgac gacccgcatc ccgatgcagg cgacgtgtac agccgccttg
     7081 cccgccgcct gaccaactgg ctttccaccg ccaccggcgc aggcagcctc tacgaaaccg
     7141 acctgcgcct gcgccccaat ggcgatgcag gtttcctcgc ccacagcatc gccgcctttg
     7201 aaaaatacca gcgcgaaaac gcctggacgt gggaacacca atccctcacc cgcgcccgct
     7261 tcatctgcgg cacacccgaa attcagacgg ccttcgaccg catccgcacc gaaatgctga
     7321 ctgccgaacg cgaccaaacc gccttagcag gcgaaattat cgaaatgcgc gaaaaaatgt
     7381 tccccaccca tccgcctgcc gacagcaacg tcaaatacgc gcgcggtggc gtggtcgatg
     7441 tcgaatttat cgtccaatat ctgatacttg cccatgcccg ccagtatccg caactcttgg
     7501 acaactacgg caacatcgcc ctcttaaaca tcgccgccga ctgcagcctt atcgacaaaa
     7561 acctcgccga acaaagccgc accgcctacc gcttctaccg ccggcagcag cacaacacca
     7621 aactgcgcga cgcggcaaaa accgaagtaa ccggcgaact gttggcacat tacggcaatg
     7681 tcaggaaatt gtggcgggac gtgttcggcg aagaagcggc aaccgcctga acaaaaaatg
     7741 ccgtctgaag cctgacaatc tgggtttcag acggtatttt cgtaccgtgc cgttttaagg
     7801 ttgcggcaga gctaaagcga tttatcggga atggctgaaa cccaaaaacc ggattcctct
     7861 ttcgcgggaa tgacgggatt tcaggcttct gtttttgtgg gaatgatggg attttttatc
     7921 caagcaaaaa tcaaaacaaa caaataaga
//
LOCUS       DAXHPG010000095         7868 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXHPG010000095 DAXHPG010000000
VERSION     DAXHPG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.095"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..325
                     /locus_tag="ACFM6F_001457"
     CDS             <1..325
                     /locus_tag="ACFM6F_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6164853.1"
                     /translation="EEGIAQTVVKGKTTRAEVEARFGKRNPFGCYAYHEVSLPIYNFL
                     PTNFIYMKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVIRKEAGKSL
                     SQPEK"
     gene            389..1672
                     /gene="hemL"
                     /locus_tag="ACFM6F_001458"
     CDS             389..1672
                     /gene="hemL"
                     /locus_tag="ACFM6F_001458"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC6164854.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFAADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1888..2160)
                     /locus_tag="ACFM6F_001459"
     CDS             complement(1888..2160)
                     /locus_tag="ACFM6F_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HFC6164855.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2171..2734)
                     /gene="orn"
                     /locus_tag="ACFM6F_001460"
     CDS             complement(2171..2734)
                     /gene="orn"
                     /locus_tag="ACFM6F_001460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC6164856.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2752..3639)
                     /gene="prmA"
                     /locus_tag="ACFM6F_001461"
     CDS             complement(2752..3639)
                     /gene="prmA"
                     /locus_tag="ACFM6F_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC6164857.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTAAQECGLKDLAYTGETLEDQDWVR
                     LTQAQFDPIQISERLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGRDNAAQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGVKR"
     gene            complement(3757..5118)
                     /gene="accC"
                     /locus_tag="ACFM6F_001462"
     CDS             complement(3757..5118)
                     /gene="accC"
                     /locus_tag="ACFM6F_001462"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC6164858.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEEERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRIQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAITGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(5231..5686)
                     /gene="accB"
                     /locus_tag="ACFM6F_001463"
     CDS             complement(5231..5686)
                     /gene="accB"
                     /locus_tag="ACFM6F_001463"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC6164859.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(5668..5925)
                     /locus_tag="ACFM6F_001464"
     CDS             complement(5668..5925)
                     /locus_tag="ACFM6F_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC6164860.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6042..7082
                     /gene="queA"
                     /locus_tag="ACFM6F_001465"
     CDS             6042..7082
                     /gene="queA"
                     /locus_tag="ACFM6F_001465"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC6164861.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPMQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDSHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAAHETGHLKDGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
     gene            complement(7559..>7868)
                     /locus_tag="ACFM6F_001466"
     CDS             complement(7559..>7868)
                     /locus_tag="ACFM6F_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6164862.1"
                     /translation="KLYQSDGRHSVNPTEGYGTGGLLQGKKHMLSLTWNAPIEAFTRE
                     GDFFEGKGVDVLYMHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG
                     "
BASE COUNT         1663 a         1963 c         2315 g         1927 t
ORIGIN      
        1 ggaagaggga atcgcacaaa cggttgttaa aggcaaaacg actcgggcgg aggtagaggc
       61 acgtttcggg aagcgcaacc ctttcggttg ttatgcctat catgaagtca gcctgccgat
      121 ttataatttt ttgccgacca atttcattta tatgaaatca gaacgccggc attgggagtg
      181 gtgtgtggat tacgactggg aaggagttgt ccgggactac cgctttacac ataaagtgga
      241 aaaagacgag cgttccgtca tccgggatgc ggttggcgtc atccgcaaag aagcaggcaa
      301 atccttgtcg caacctgaaa aatgataaaa tgggactttc tgcttctaag cccgaaacct
      361 gccgttcaga cggcatttga ggataaatat gaaccgtaac gaaattttat tcgaccgcgc
      421 caaagccatc atccccggcg gcgtgaattc ccccgtccgc gcattcggca gcgtcggcgg
      481 cgtgccgcgc ttcatcaaaa aagccgaagg cgcgtatgtt tgggacgaaa acggcacgcg
      541 ttataccgac tatgtcggct cttgggggcc tgcgattgtc ggacacgcgc accccgaagt
      601 cgtcgaagcc gtgcgcgaag ccgcgttggg cggtttgtcg ttcggcgcgc ccaccgaggg
      661 cgaaatcgcc attgccgaac aaatcgccaa aatcatgccg tccgtcgaac ggctgcgcct
      721 cgtcagctcc ggtaccgaag cgacgatgac cgccatccgt ttggcacgcg gttttaccgg
      781 tcgcgacaaa atcatcaagt ttgaaggctg ctaccacggg cattccgaca gcctgctggt
      841 gaaagccggc agcggcctgc tgacgttcgg taatccctct tccgccggtg tgcctgccga
      901 ctttaccaaa cacactttag tccttgagta caacaacatc gcccaattgg aagaagcctt
      961 tgctcaaagc ggcaatgaca ttgcctgtgt gattttggag ccttttgtcg gcaatatgaa
     1021 ccttgtccgc ccgaccgaag ccttcgtcaa agccctgcgc gaattgaccg aaaaacacgg
     1081 cgcggtgttg atttacgacg aagtgatgac cggtttccgc gtcgcgctcg gcggcgcaca
     1141 atcgctgcac ggcatcatgc ccgacctgac cacgatgggc aaagtcatcg gcggcggtat
     1201 gccgcttgcc gcgttcggcg ggcgcaaaga cattatggaa tgtatttccc cactgggcgg
     1261 cgtgtatcag gcaggtacat tatcaggcaa cccgattgcc gtcgccgccg gtttgaaaac
     1321 gctggaaatc atccgccgcg aagggtttta tgaaaacctg acggcgcgta ccgaacagtt
     1381 ggttcaaggt tttcagacgg cagcggatgc ggcgggcatc gagtttgccg ccgacagcgt
     1441 gggcggtatg ttcggtctgt atttcgccgc acacgcgccg cgaaactatg ccgatatggc
     1501 gcgttccaat attgacgctt tcaaacgctt cttccacggt atgctcgacc gcggcattgc
     1561 cttcggcccg tccgcttatg aagcaggttt cgtttctgcc gcgcatacgc ccgagctgat
     1621 tgacgaaacg gttgcggttg cggttgaagt gttcaaggcg atggctgcat gatgttttga
     1681 cggacagagt ttctctgttc gatttgtttg gcagattgaa gtaagaatgc acaccgccgt
     1741 cattcccgcg caggcgggaa tccagacctt tcagtttctg taatgattga aaataacggg
     1801 ccgaaatcag ccgcgcccgg caggaagccg ccgccacctg aaacaatgcc gtctgaagcc
     1861 ttcagacggc attttatcgg aagcgcatca gaaaaaacgg atagcgacat atgcggtaaa
     1921 agcaagggca attacataga taaacccctt tataaattca aaagtctcat ctatatcgct
     1981 atatttttct ttcagttcgt cagattttcg ataacgtttc attttcaaga ccacttcatg
     2041 accaacaagc ccgaccttcc ggattcccgc ctgcgcggga atgacgggcg tgtatatttt
     2101 tgatttcaat ctgctgtaaa aatgccgtct gaaatgcagg atttgcgctt cagacggcat
     2161 tttttgtttc ttattgcgct tcggctctcg gggcggaaat cagaaagtgt tcgcggtagt
     2221 ggcgcatttc ttcgatgctc tccaaaatgt cgtccaatgc cttgtgcgaa ccgcgtttga
     2281 cgacgctttt ggcaacgggc ggattccagc gtttggcaag ctctttcagt gtggaaacgt
     2341 caaggttgcg gtagtggaag tagttttcca gtttcggcat atatttgacc ataaaacgcc
     2401 ggtcttggtg gatggaattg ccgcacatcg gcgtggcgcg tccgggtacc cattccgaca
     2461 taaagtccag cagtttctgt tcgacttcgg cttcggtatg cgacgattcg cgcacgcgct
     2521 gcgtcagccc cgtcctgccg tgtgtggcgg tgttccactc gtccatattg tcgagcagct
     2581 cgtcgctttg gtggacggcg taaacttcgg attgcgccaa tacattcaaa tccgagtcgg
     2641 taataatcat cgcgacttcg ataatgcggt cggtttcggg attcagcccc gtcatttcca
     2701 tatcgagcca gcagaggttg tttttgtcct gcacggtgtt tccttttcgt ttcagcgttt
     2761 tacgccgctc aagcgcgccc agccttcttc ggtttccgcc gggtcgaggt cgaaccattg
     2821 gctgtaaatg ccgccgagtt cttcggcctg ttcgtccaac aaaccggaca acacgatgcg
     2881 tccgccctgt ttggtgcggg ctgcgagcat ttcgccgagc atacgcaaag ggttggcgag
     2941 gatgttggcg acaactacgt cgaattgccc ttgaggcaga ccgtcgggca ggaagaattg
     3001 tgcatcgacg ttgttttgcg cggcgttgtc cctgccggcg cggacggcct gttcgtcaat
     3061 atccacgccg acggcggaac ctgcaccgag tttgagggcg gcgatggtca ggatgcccga
     3121 accgcagccg tagtcgagga cgctttcgcc gtttttgagt tgcgtatcca gccatttgag
     3181 gcagaggcgc gtggtcgggt ggctgccggt gccgaaggcg aggccgggat cgaggcggag
     3241 gttgacggca gtgccttcgg gggcttcgtg ccaagagggg gtaatccaca ggcgttcgga
     3301 aatttggatg gggtcgaatt gcgcttgggt caggcgtacc cagtcttggt cttcaagggt
     3361 ttcgccggtg tatgccaagt cttttaatcc gcattcttgt gcggcggttt ggatgatggc
     3421 ggcggcttcg tcgtgttcgc cgaacagggc gatgactttg ctctgctgcc agatttgttc
     3481 ggcgggcata ccgggttcgc cgaaaatcgc ctgttcgttt tgcgtgccgg cgtaggcatc
     3541 ttcgatggcg gcggagagtg cgccgtgttc catcagcgcg tcggcgaggc gttcggcgac
     3601 ggcatcgttg acgttgacgg tgatttgttg gtagggcata acgggctttc ttaacagacc
     3661 ttcggaatag ggtcgtactg tataagggga tgggtgtgta acggaaatgc cgtctgaaaa
     3721 cggacaaact gtttcagacg gcatatcggc ggcggtttac ttgtcctgtt tggctttgcg
     3781 atcttccagc cagtgttcca aatagtggat gctgacgccg cctttttgga aacccgcatc
     3841 ggcgaacagg tcgcggtgaa gcggcgtatt ggttttgatg ccggtaattg ccagctcggc
     3901 gagggcgacg cgcatttttg ccattgcctg ttcgcgggtt ttgccgtgta cgcagatttt
     3961 gccgatcagg ctgtcgtagt aaggcgggat gcggtagcct tggtagatgt ggctgtctac
     4021 gcggatgccg aagccgccag gcaggtggca gctttcaatc gggcccgggc ttgggatgaa
     4081 gttgtacggg tcttcggcat tgatgcggca ctcgaaggcg tggccttcga cttgaatatc
     4141 cttttgtttg tattgcaaag gcaggccgga tgcgatgcgg agttgctctt gtacgatgtc
     4201 cacgccggtg atgagttcgg taaccggatg ctcgacctga atgcgcgtgt tcatctcgat
     4261 aaagaaaaat tcgccgtctt cgtataaaaa ctcaaacgta cccgcgcccc ggtagccgat
     4321 gcgtttgcag gcgtcggcac aggcgttgcc gattttggcg cgttcttctt cagtgatgaa
     4381 cggagccggt gcttcctcga tgactttttg gtggcggcgt tgcagcgaac agtcgcgctc
     4441 ggcaagatat acggcgttgc cgtgttcgtc ggcaatcact tggatttcga cgtggcgcgg
     4501 acgttgcaga tagcgttcca tgtaaaccat tgggttgccg aatgccgcgc ctgcttctgc
     4561 cttggtcatt tcgacagatt ggaggaggtc ttcttttttc tcgaccacgc gcataccgcg
     4621 accgccgccg ccgccagagg ctttgataat gacgggataa ccgactttat cggcgatttt
     4681 gaggatttct tcgccgtcgt cgggcaatgc gccgtcagaa ccggggacgc agggtacgcc
     4741 tgccgctatc atcgcgtgtt tggcggagac tttgtcgccc atcaggcgga tggtgtcggg
     4801 tttcgggccg ataaaggtaa agccggactg ctcgacctgt tcggcgaaat cggcgttttc
     4861 ggcaaggaaa ccgtagcccg gatggacagc gtccgcgcag cttacttcgg cggcggcgat
     4921 aatggcgggg acgttaaggt agctttgcgc ggaagcggca gggccgatgc acacggattc
     4981 gtcggcgagt ttgacgtgca ggctgtcttt gtcggcctcg gaatgcacgg cgacggtggc
     5041 aatgcccatt tcgcggcagg cacggagtac gcgtaatgcg atttcgcctc ggttggcgat
     5101 taaaactttt ttcagcatga tgactgttcc tgtggttccg gcaacccgtc cgtaaaaaaa
     5161 aggtgcggga acgctgaagg gggaaagcat ttcagacggc atcggaagtt tatgccgtct
     5221 gaaaacagga ttatccgatg atgaagagcg gttcgccgaa ttcgacgggc gtaccgtttt
     5281 cgaccaaaat ttctttgacc gtgccggatt tttcggcttc gatttcgttc atcagcttca
     5341 tcgcttcgat gatgcacagc gtgtcgccgg ctttaacctg ttggccgact tcgacaaaag
     5401 gcgcggcatt cgggccgggt gcgcggtaga acgtgccgac cataggtgat ttttgcgcgt
     5461 cggacaaatc gcgggcggca ggtgcggcgg cgggcgcgga tgccgcaacg ggtgcggcgg
     5521 caggcgttac ggccggcgcg gcggcaggta cgggcgcggc gtaaacgggt gcggcggcga
     5581 tggttcgggt gatgcggact ttttcttcgc cttcggtaac ttcgatttcg gcgatacccg
     5641 attcttcaac caaatcaatc agttttttta atttgcgcaa atccatttct gttcctttaa
     5701 aggctgccgg tgttccggcg ggttgttgcg ttggtttttc agaaaacagc cggcttgcgg
     5761 caacggcgca aaccgggcgg aatggtgttc ggatacggtt ttcctgtggt ggaaaacaac
     5821 cgtgagaatg tgtttatttt cccgaagttg tcggtaaatg tccagtaaaa tatcaaaaaa
     5881 cggcggtttt tgggggaaat gtgcaggaag tttgattttg cgcacaagat gttccaggtt
     5941 aagagtgttt attctaatct gttggttttt cggacaaaga tgccgtctga aaaagggcta
     6001 aagtgcgtat aatggcggct tgcccgaacg agagtgtaaa aatggatatt tcagattttg
     6061 attttaccct gcccgaaaag ctgattgccc agcatccgcc cgaagtgcgc ggcagcagcc
     6121 gccttttggt tgcgctgccg gatatgccca tgcaagaccg ggtattcggc gatttgcccg
     6181 attatgtcga ggcaggcgac gttttggtgt tcaacaacac caaggtaatg aaggcgcggc
     6241 tgtttgggca gaaagacagc ggcggcagga tcgaagccct gatcgagcgt gttttagaca
     6301 gtcataccgc actggcgcac atccgttcgt ccaaatcgcc caagcccggt atggggctgg
     6361 tgtttgaagg cggcatccgt gccgtgatgg tcgggcgtga gggcgaactg ttctgcctgc
     6421 gttttgaagg cggtcaaacc gtttacgaac ttttggaaca gaacggacat ctgcccctgc
     6481 cgccttatat cgaacgtgcc gccgatgcgg atgacgacag ccgttatcaa accgtttatg
     6541 ccaaatatca gggtgcggtc gccgcgccga cggcgggtct gcattttacg gaagaacttt
     6601 tgcgccgcct gaaagacaaa ggcgcagtaa ccgcagaagt aaccctgcac gtcggtgcgg
     6661 ggacattcca acccgtgcgt gtggaaaaaa tcgaagaaca caaaatgcac agcgaatggt
     6721 ttgaagtgcc gtctgaaacc gccgccgccg ttgaggcggc aaaagcccgg gggaacaaag
     6781 tctgggcggt cggtacgact tccatgcgcg ccctcgagtc tgccgcgcac gagacgggac
     6841 acttgaagga cgggcagggc gacaccgata ttttcatcac gccgggctac cgttttaatg
     6901 ttgccgacag gctggttacc aatttccatc tgccgaaatc gacgctgctg atgcttgtca
     6961 gcgcgttttc gggtatgggt catatccgcg ccgcgtatcg ttatgcggtc gaacgcgaat
     7021 accgtttctt cagctacggc gatgcgatgg ttttggggcg gaacgaaggg gtggtacgtt
     7081 agaacagcct caagtctatt gggggaagac aagtgccgtc tcaacggacg ttcggacggc
     7141 attcttttgt tgatttgaat gaaaggaact gtatgacata tagtgtggat taaatttaaa
     7201 tcaggacaag gcaacgaagc cgtagacagt acaaatagta tggcaaggcg aggcaacgct
     7261 gtactggttt aaatttaatc cactatagat tcaggcgcga gaggcgagaa actcagtcat
     7321 tgttgatgac gcgcaatacg ttaaaattca ttccgttttg gtcgtataaa catattacat
     7381 acgccatttt tttgtattca aggctgcctg aaaacagtgg gcaagaatat ctaacccaac
     7441 gaaaagctga aaaagcaaca aaaaataatc tgcaccttag tcggttggtt agtccaacct
     7501 taggggtgca gaccattcaa tctttcaggc tgcctttgtt tgtttataag ataaattttt
     7561 agccgaacac tttttccaag tgtgcctgat aatctgccaa gtatttttcc acttgcggat
     7621 ttttaaccac atcgttacat aagaatgtcg gcaggcgggt catacccaaa aactcgttgg
     7681 ctttgtggaa gtgcatatac aaaacatcaa cgcctttgcc ttcaaagaaa tcgccttcgc
     7741 gggtaaacgc ctcaatcggc gcattccaag tcagtgaaag catatgtttt ttgccttgca
     7801 acaagccgcc tgtgccgtag ccctcagtcg gattgacgct gtgtctgccg tcgctttggt
     7861 agagtttg
//
LOCUS       DAXHPG010000096         7862 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXHPG010000096 DAXHPG010000000
VERSION     DAXHPG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.096"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            27..1142
                     /gene="asd"
                     /locus_tag="ACFM6F_001467"
     CDS             27..1142
                     /gene="asd"
                     /locus_tag="ACFM6F_001467"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC6164863.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIEAILAGANDWVKVIPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            complement(1371..1901)
                     /locus_tag="ACFM6F_001468"
     CDS             complement(1371..1901)
                     /locus_tag="ACFM6F_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6164864.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAVSYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIQGRHIAVVAHGAGAAAFLAWLYRADILTRKKIANIILVPQHPD
                     IFSDDAEHTFQRVRCPCRAALVVSEHGDVPRDWAQKQADTWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(1912..2256)
                     /locus_tag="ACFM6F_001469"
     CDS             complement(1912..2256)
                     /locus_tag="ACFM6F_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164865.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFVLPMSVWAIFMLARM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWKGWRRVVNIFVCLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            complement(2243..3022)
                     /locus_tag="ACFM6F_001470"
     CDS             complement(2243..3022)
                     /locus_tag="ACFM6F_001470"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6164866.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHTLGWTDMWRTLYPDNPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            complement(3193..4614)
                     /gene="cysS"
                     /locus_tag="ACFM6F_001471"
     CDS             complement(3193..4614)
                     /gene="cysS"
                     /locus_tag="ACFM6F_001471"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC6164867.1"
                     /translation="MTTIYNTLTRQKEPFTPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGRGRPGWHIECSAMSENLFG
                     NTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPIEFLQGGAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            complement(5302..6351)
                     /locus_tag="ACFM6F_001472"
     CDS             complement(5302..6351)
                     /locus_tag="ACFM6F_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6164868.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSRLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEVNLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            complement(6434..7588)
                     /gene="obgE"
                     /locus_tag="ACFM6F_001473"
     CDS             complement(6434..7588)
                     /gene="obgE"
                     /locus_tag="ACFM6F_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC6164869.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPIGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETANPAEEALAIINELRKYDEELYD
                     KPRWLVLNKLDMLDEEEAQARSAAFLEAIGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
BASE COUNT         1788 a         2043 c         1980 g         2051 t
ORIGIN      
        1 acgatttttc ctcaaggagc aacacaatga aagtaggttt cgtcggctgg cgcggtatgg
       61 tcggttcggt tttgatgcag cgtatgaaag aagaaaacga cttcgcccac attcctgaag
      121 cgtttttctt taccacttcc aacgtcggcg gcgcagcccc tgatttcggt caggcagcca
      181 aaacattgtt ggatgccaac gatgttgccg aattggcaaa aatggacatc atcgttacct
      241 gtcagggcgg cgattacacc aaatccgtgt tccaagccct gcgcgacagc ggctggaacg
      301 gctactgggt tgacgcggca tcttctctgc gcatgaaaga cgacgcgatt atcgtcctcg
      361 accctgtcaa ccgcaacgtt atcgacaacg gcctcaaaaa cggcgtgaaa aactacatcg
      421 gcggcaactg caccgtctcc ctgatgctga tggctttggg cggcctgttc caaaacgatt
      481 tggtcgaatg ggcaaccagc atgacctacc aagccgcttc cggcgcaggt gcgaaaaaca
      541 tgcgcgaact catcagcggt atgggcgcgg ttcacgccca agtggcggac gaacttgccg
      601 atcctgccgg ctcgattctc gacatcgacc gcaaagtatc cgatttcctg cgcagcgaag
      661 actatccgaa agccaacttc ggcgtaccgc tcgccggcag cctgattccg tggattgacg
      721 tggatttggg caacggccag tccaaagaag aatggaaagg cggcgtggaa accaacaaaa
      781 tcctcggccg cagcgacaat ccaaccgtga ttgacggcct gtgcgtccgc gtcggcgcga
      841 tgcgctgcca cagccaagcc atcactctga agttgaaaaa agacctgccc gtttccgaaa
      901 tcgaagcgat tttggcaggc gcgaacgact gggtgaaagt catccccaac gaaaaagaag
      961 ccagcatccg cgagctgacc cccgccaaag ttaccggcac gctgtccgtc cccgtcggac
     1021 gcatccgcaa actgggtatg ggcggcgaat acatcagcgc gttcaccgtc ggcgaccagc
     1081 tcttgtgggg cgcggccgag ccgctgcgcc gcgtattgcg tatcgtgttg ggcagcctgt
     1141 aagccctgtt tgaatggaaa tgccgtctga agcctgtttc agacggcatt ttccttgcaa
     1201 ccctgccgga taacgccctg ccggcactgc cgacgtaaaa aataaaggat tccatttccg
     1261 gcggtatgcg gcagcccgac tttatccgaa cctgatgcgc ctgcacgtca atgaaaacag
     1321 cccgatatgc ggacttcctg ctacagccga aattccgata aggcaagcgt tcacgccagc
     1381 aacatttcct gcatcagctt cataccccac tgccagccgc cgagcatacc gttcaaactg
     1441 cccgaatgcg gggaaaccaa caggcgcgcg ttccacgtat ccgcctgttt ttgcgcccaa
     1501 tctcgcggca catccccgtg ttcggacacc accaatgcgg cacggcaggg acagcggacg
     1561 cgttggaaag tgtgttccgc atcgtcggag aaaatatcgg gatgctgcgg tacaaggatg
     1621 atgttggcaa ttttcttccg tgtcaggatg tctgcgcggt acagccacgc caagaatgcg
     1681 gccgcacccg cgccgtgtgc gacaacggcg atatgcctgc cctgtatccg ttcgaatgcc
     1741 gtctgaagcc ctgcctgcca ttcccctatg ctttgaccgg ccgatgcttc ggacatctgc
     1801 acgacgggat aactgaccgc ccaacggtct atccacatct gatcttctcc ggcatcgcgt
     1861 atcagccaaa gcgtcaaatc ttcgagttcg aaaccctgca taccgccgcg cctatttcag
     1921 caggtcccgg agggtaaagg cgatgagcag cgaagcgggg acgctcaata tggcgcagac
     1981 ggtcaggcag acaaaaatat tcaccacccg ccgccagcct ttccaaccca aacatttgga
     2041 cgcaatcagc aggcagggca ggcaaatcag cagccacacc aacgcccata tcgggtttgc
     2101 cttggtcggc gcaagccagc cctgcatccg cgccagcata aatatcgccc acaccgacat
     2161 aggcagcaca aatgccgcca cgacccatgc cgcgcctatt cctgtttttc cgtccacatt
     2221 ccaatcatat ttacccaaaa ctttattcgg cagcatagtc atactccacg accagcggcg
     2281 catggtcgga aaatttttcg tctttataaa catgtgcgga cacggctttg gcggcaagtt
     2341 cgggcgtaac catctgataa tcgatgcgcc acccgacatc tttcgcatac gcctgcccgc
     2401 ggttgctcca ccaagtgtag cccggattgt cgggataaag cgtgcgccac atatccgtcc
     2461 agcccagcgt atggatgacc ttgcctatcc actcgcgctc ttcgggcagg aagcccgaat
     2521 ttttctgatt gcccttccag tttttcaggt cgatgttttg gtgggcgatg ttccagtcgc
     2581 cgcagacgac aatgtcgcgc ccttcgtttt tcatcgcttc gagcataggg taaaacgcat
     2641 cgaggaaacg gtatttcacc tgctggcgtt cttctgcgct gctgccgctg ggcaaataaa
     2701 gcgaaataac gctcaacctg ccgaaatcgc aacgcacaaa ccgcccttcc ctgtcgaatt
     2761 cttcaatgcc cataccgatt tgcacattgt cgggtttgcg tttgctgtac accgccacgc
     2821 cgctgtaacc acgcttctcg gcgcaatgcc aatagccgtg catcccgtgc ggatttttca
     2881 tatcggcaga caaatcagcc tcctgcgctt tgagttcctg cacgcagaca atgtccgcgc
     2941 ccgatgcggc gatatattcg taaaaacctt ttttgtaggc ggagcggatg ccgttgacgt
     3001 tggcggaaat gattttaagc ataataaaaa taagttctca caatgttccc atacacaaag
     3061 cagcacccgg tgcgccgccc ggcatttccc attcggccct gcctgctgaa aacggcgggg
     3121 cttcaagcgg gaaatgccgt ctgaaaacgg aaaaagagca aaaggcggta cttttgccct
     3181 ttccgataca gttcaaccgc gccgccaagt cgtaccgccg gcgttgtctt ccagaatgat
     3241 tttgtgttcg ttcagaaggt cgcggatgcg gtcggactct gcccagtttt tatccgcgcg
     3301 cgcttgtttg cgccgtgcga ttaagtcttc gatttcttcg ttggagaggc cgtctgaagc
     3361 cgcgcctcct tgcaggaact caatcggatc gcgttgcagc agtccgatga tgccgcccaa
     3421 ggctttcagg cagccggcga gctgtgcgtc attggttttg ttcacttcgc ctgccagttc
     3481 gaacaacacg gcaaccgctt cgaccgtacc gaaatcgtcg ttcatggcgg cgtagaagcg
     3541 gcgggtgtag ccgttggcgt tttcggataa atcaaactca gccgccggcg tgtttttcaa
     3601 ggtggtgtac agacgggtca gcgcaccttt tgcatcgtcc aaatgcgcgt cggaatagtt
     3661 caacgggctg cggtagtggg cgcgcaggat gaagaagcgc acgacttcgg ggtcgtattg
     3721 tttcaacact tcgcggatgg tgaagaagtt gcccagtgat ttggacattt tttcgccgtc
     3781 cacgcggata aagccgttgt gcagccaata tttgacgtgg ctggcgatgc tttgcccgtg
     3841 gtgggtttgc gcgtgatgat gaccgcaggt atgccccgtc gcgccgacgc tttgggcaat
     3901 ttcgttttcg tggtgcggaa actgcaaatc cgcgccgccg ccgtggatgt cgaaggtatt
     3961 gccgaacagg ttttcactca tcgcagaaca ttcgatgtgc cagcccggcc gtcccctgcc
     4021 ccacgggctt tcccatgccg gctcgcctgc tttcgccgct ttccacaaca caaaatcaag
     4081 cggatcgcgt ttgaaaccgt ccacttccac acgttcgccc gcgcgcaggt cgtccaacga
     4141 tttgcccgac aattggccgt aagcggcaaa ctcgcgcacg gcgtagtaaa cgtcgccatt
     4201 tgcggcagga tatgccttgc cgttttgaat cagggtttca atcatggcaa tcatttgtgg
     4261 gatgttttcc gtcgccttcg gctcgatatc gggacgcaac acacccaaag catcggcatc
     4321 ttcgtgcata gcctgaatga aacgcgcggt cagttcgccg atggtctcgc cgttttcagc
     4381 cgcgcgggca atgattttgt cgtcgatgtc ggtgatgttg cgcacataag tgagcggata
     4441 accgcactcg cgcagccagc gggcaatcat gtcgaacaca accatcacac gggcatggcc
     4501 taagtggcaa tagtcgtaaa cagtcatgcc gcaaacgtac atacgcacgt tttcagggtc
     4561 gatgggggta aagggttctt tttggcgggt aagggtgttg tagatggtgg tcatgggatt
     4621 atggattaat ctttgttgct cggatgataa tttctgttct gttcctgtag atacggacca
     4681 aggaacatta cgtagttgcg gattattaat atggctgata tttgtgaaaa ttggttctgc
     4741 ataacagttt gcaaaatttt ttgtaaattc tgataattta aacttatctt ttaataagtt
     4801 tgctaaatct gatgacgagg gataaagttt acttcttata ctaggcattt caatatgaag
     4861 gactattttt atttcgttac aatctaaagc caagcgagaa aaatcttttt cttcctgttt
     4921 ttctgcttta aatttagcag aaaccaatcc tgccaatgaa tctcgaattt ttcttgcgat
     4981 taaatatggt aggtcagaaa gtttttcatc tattgattgg gcatttggct caagcccaag
     5041 tcggtaataa tctttaattt cgattagcca aagtgtcgat tcatgaaggg ctattatatc
     5101 tacacctgag ctgccattat cgtcatctac actttgattt atctcgttct ttcccttttc
     5161 atttgtatca attttattac gtaaattaca actgttctga aaaattttat aatgttccca
     5221 ttcgtcatac ttggtaacgt aataatcttc aggaaaagca aaggttaatc tcttttctgt
     5281 gattgtagtc atagcttaac ctcaaatatt cagatacctg tctgcctgca taatgttttc
     5341 atctaacaat atcaatgtgt tcaaatcatt aatactgttc ccttgctcca cttttgttcc
     5401 atcatcggaa gcaatcaacg agaaaaaacg tacaggtaaa tccgtgttat tttcaagctt
     5461 caaaagttcc aattctctca ataagaataa agagtgtgtt gcaataaaaa cctgaatacc
     5521 ctgttgagat aaagaccaaa taatacgggc agccactttg atcaatttag gattcagatt
     5581 aacttccggt tcatcccaaa atagatagcc tttatccagc aatgcccctg ttgcgattaa
     5641 ccgggcaatc atgacaaatt tccgcaaacc ctctgctacc aaaggtgctt caatcttacc
     5701 gcccgtattt gtcagcgata gataaaacct tccttgttct tcagatactt ttccgcccat
     5761 cgcgttctca ataggttcga gcaattctcg aatttttgtt tctctggggc ctttggcaag
     5821 cgggtgattt aattgcatac aggcatcaaa ccaagtttct tcgaaaggga tgctttggtt
     5881 ttgatacaaa gaagtgaacc aagggcaaag tgtaattaat tcgcggctgg gtaagaagat
     5941 aggtgtcgga gtatattcat tttctttcaa tccgatgctt tgaacattga cttgcgatga
     6001 tgagttactg gaaaaattca gcctactatg cgtagtgccg ttttgcagtt ttaaaacgat
     6061 ttccgtacgc ccgcgcccct gcaaacgttt gctcaacctg cccaaggaat cgggacggaa
     6121 aacattcagt aatttatcgg caaaactttt ttgcaattct gttttcagta atctgttttt
     6181 ggtgttagat gttacttcta gcaggctgta taaaattttt aacaaatgtg ttttgccaca
     6241 accgttttcg gcaacaataa cattgagatt ttcagaaaat tcaaaagtat cgtttggaag
     6301 aacggtaaag tttgtcaact caagcgactg gatatattgg ttagatgaca tttttaatcc
     6361 atttcaatct tgctttaaaa ttgtttcaaa caaccttttg tagaacaaat atcgtctgaa
     6421 accctttctt ttttcactcc ggcttaaaca cgcccgtatc cgttttaggc tgctgttcga
     6481 taatttcaac atttgccgct gctttctccg cttcggcttt ttcagcttcg atacgttttt
     6541 tctcggtcag gtattggttg atttggtgta ccaattcctg cgtgccttgg tgggtcagcg
     6601 cgctgatttg gaagaggcgc ggggtttcca tgtcaaattg gaaacggtcg tcgggtttcg
     6661 ggtagtccca gccgatggct tcgaggaagg cggcgctacg cgcttgggct tcttcttcat
     6721 cgagcatatc gagtttgttc agcaccagcc agcgtggttt gtcgtagagc tcttcgtcgt
     6781 atttgcgcaa ttcgttgatg atggcgaggg cttcttcggc ggggttggcg gtttcgtcga
     6841 agggtgccaa atcgacgacg tgcagcaata atccggtgcg cgataagtgt ttgaggaaac
     6901 gatgccccaa acccgcgcct tctgccgcgc cttcaatcag gccggggatg tcagccatca
     6961 cgaagctgtg gttttcgtcg atgcgtacca cgcctaagtt tggatgcagg gtggtgaagg
     7021 ggtagttggc gattttgggg cgtgcggcgg atacggcggt aatcagggtg gatttgccgg
     7081 cgttgggcat acccaataag ccgacatcgg cgaggacttt gagttcgagt tgcagggagc
     7141 gggtttcgcc ttcttcgccg ggcgtggatt gttttggggc gcggttgacg gaggatttga
     7201 agtggatgtt gcccaagccg cctttgccgc ctttggcaag gcagacgcgc tgaccgtggt
     7261 aggtgaggtc ggcaacgatt tcgtcggtgt cgaggtcgcg gataagggtg ccgatgggca
     7321 ttttgaggac gatgtcgtcc gcacctgcgc cgtaacgatc ggagccgtgg cctttttcgc
     7381 cgtttttggc ttggtagcgt ttaacgaagc ggtattcgac gagggtgttg gtgttttcgt
     7441 cggcttccgc ccagacgcta ccgcctttgc cgccgtcgcc gccgtccggg ccgccgcgcg
     7501 gtacgaattt ttcgcggcgg aaactggttg cgccattacc gcctttgcct gcggcgactt
     7561 cgatttttgc ttcgtcgatg aatttcattc aatgctcttg tttgttggtt tcaaatgggg
     7621 ggttcagacg gattaccgtg tgttttgatg ccgtccgaac agaatttcgg acgctattat
     7681 aagggataag cggtatttca acacgccgta cccaaactat ttgttccgcc catcttaatg
     7741 aatttttaag caaatcttca gcctgcaaac aaaattatgt ccaacttctt tggtacaatc
     7801 gcgccttttt gacattccga cccgacggaa tgtccgttca aaccgttaca tataataagt
     7861 tt
//
LOCUS       DAXHPG010000097         7801 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXHPG010000097 DAXHPG010000000
VERSION     DAXHPG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.097"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(210..1595)
                     /locus_tag="ACFM6F_001474"
     CDS             complement(210..1595)
                     /locus_tag="ACFM6F_001474"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC6164870.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNIRGNQVLPKHPNGLRFTAYASDGIVLKEQVYYSTGGGFVVTGEDFERQSETEK
                     TVPYPYNSCAELLDRCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(1694..4084)
                     /gene="gyrB"
                     /locus_tag="ACFM6F_001475"
     CDS             complement(1694..4084)
                     /gene="gyrB"
                     /locus_tag="ACFM6F_001475"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC6164871.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSSEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRIIDELVLHAMLHASPVDLSTAESA
                     DRSVAELNGLLDEKEVALERIEGHEGHQFIKITRKLHGNVVVSYLEPKFLNSKAYQTL
                     TQTAAALKGMVGKGAKLYKGDNEYDIDSFEGALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            4354..5226
                     /locus_tag="ACFM6F_001476"
     CDS             4354..5226
                     /locus_tag="ACFM6F_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC6164872.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAVVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            5314..7350
                     /locus_tag="ACFM6F_001477"
     CDS             5314..7350
                     /locus_tag="ACFM6F_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC6164873.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDGGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSTHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
BASE COUNT         1658 a         2184 c         2163 g         1796 t
ORIGIN      
        1 tcttgccgga tgctgcaaac aacgccggtt tgcattcctg atggcggtgg ttttcttaga
       61 cgaacgcccg aacacgcagg aatggatagg cttggggctg gttacggcgg gcgtgttgac
      121 gctggcactg aaacggtaaa gccgcaagaa ataaatgaaa tgccgtctaa aaaactgttt
      181 tcagacggca ttttcgtttc tgtccatcct cagcactcga ccacgcgcac ggatacgggg
      241 acggcttttt tccggagcgt ggcggcgagt ccggcaagcg aggtttcttt gtaggtcgcc
      301 ttcatatccc ggccggtttg cagcatggtt cggatgactt cgtcgagcga gacttttttg
      361 tccgtgccgt cttctaaaag cgcgagcgtg ccgagtttga gggctttttc ggcggcgatg
      421 ccgttgcgct cgatgcaggg gatttgcacc agtccgccga cggggtcgca agtcagcccc
      481 aaatggtgtt ccatcgccat ttcggcggcg ttttccactt gtttgggcgt gccgccgatg
      541 acttcggcgt atgcgcccgc cgccatcgaa cacgctacgc cgacttcgcc ctgacagccg
      601 acatccgcac cggaaatgga ggcgttggtc ttgtagagga tgccgattgc gcctgcggtg
      661 agcaggaagt tttcgacgcg ttcctgtgtg gcgtgcggat tgaacttgcg gaaatagtgc
      721 aatacggcgg gaatgatgcc tgccgcgccg ttggtcggtg cggtaacgac gcgtccgccg
      781 gcggcgtttt cttcgttgac cgccatggcg tacaccatcg gccagagctg ggtgttgacg
      841 atttcggttt cgcgcaggac tttgagcttg gcggcaagct gcggggcgcg gcggcggacg
      901 ttcaatccgc cgggcagttc gccgtccgca cccaagccgc gtttgatgca gccttccata
      961 acctcggcaa cggcagcggc gcggcggcgg atttcggctt cgccgcatcc ggcaagcgcg
     1021 gcttcgtttg ccaacacgac ttcggagatg tcgagccggt tcagacggca tcggtcaagc
     1081 agctcggcac aactgttgta aggataagga acagtttttt ccgtttccga ctgccgctcg
     1141 aaatcttctc cggtaacgac aaacccgccg ccggtcgagt aataaacctg ttctttcaag
     1201 acgatgccgt ctgaagcata ggcggtaaat cgcaggccgt tggggtgttt gggcagcact
     1261 tgattgccgc gtatgttcag gtcgcggtcg gggatgaagc ggatttcttg cccgttgagc
     1321 cggaggatgt gctgcgtgcg gatgcgttcg aggcgttcgg gaatgccggc aagcgggatg
     1381 tcgtgcggca ggctgccttc caaaccgagc atcagcgcgt caaatgtacc gtgtccgtat
     1441 ccggtcagtg cgagcgagcc gtaaatgtcg atgacgatgc gaacagcctg tgcatccaaa
     1501 cctgccgcaa aggcggcggc tgccttcatc gggccgaccg tatgcgaact ggaaggcccg
     1561 ataccgattt tgaaaatatc aaaaatgctg accatggttt tgctccgaaa ggcggtttca
     1621 gacggcatgg cttccgtttg acaaaccaaa aggagacgcg gcacgatgcc cgtctccttt
     1681 tttaaaacgg cacttatgcg tcgatatttt gggcaatcag cgcgttgttt tcgataaagg
     1741 cgcggcgcgg ttcgacctcg tcgcccatca gggtaacgaa tacttcgtcg gcggcaatgg
     1801 catcttcgat gcgcactttc aacaggcggc gcacggcggg atccatcgtg gtttcccaca
     1861 gctgttcggg gttcatctcg cccaagcctt tgtatcgttg gatggacata cctttttggg
     1921 caacgctcat caagatgtcc aaagcgcctt caaagctgtc gatgtcgtat tcgttgtcgc
     1981 ctttgtagag cttcgcccct ttgccgacca tgcctttaag tgcggcggcg gtttgggtaa
     2041 gggtttgata ggctttgctg ttgaggaact taggctcgag gtagctcacc accacattgc
     2101 cgtgcagctt gcgtgtgatt ttgatgaact ggtgtccttc gtggccttcg atacgttcga
     2161 gcgcgacttc tttttcgtcc aagaggccgt tcaattcggc aacggagcgg tctgcgcttt
     2221 ctgctgtgct gagatcgacc ggcgaagcat gtagcatggc atgcaggacg agttcgtcaa
     2281 tgatgcggct ttcttgctcg atgacggttt tggccaacag gaattgtttg gcggtattgg
     2341 caagctcttc gccttcgatg gtgcggctgt ctgaaacgat tttggctttt tccaaggcta
     2401 aaccgagcag ccattggtct ttttccaatt cgtctttgag atatcgttct tgtttgccgt
     2461 atttggcttt gtataaaggc ggctgggcaa tgtagatgta gccgcgttcg accagctcgg
     2521 gcatttggcg gtagaagaag gtcaggagca gggtgcggat gtgcgcgccg tccacgtcgg
     2581 catcggtcat gatgatgatg cggtggtaac gcagtttttc ggcattgaat tcttctttgc
     2641 cgatgcccgc gcccaacgcg gtaatcaaag cggctacttc ctgactggcg agcatttttt
     2701 caaagcgcgc tttttcgacg ttcaaaatct tgcccttaag cggcaaaatc gcttggaatt
     2761 tgcggtcgcg cccctgcatg gcggaaccgc ctgcagaatc gccctcgacc agataaagtt
     2821 ctgacaatgc ggggtctttt tcttggcagt cggcgagttt accgggtagc cccaagccgt
     2881 ccattacgcc tttgcggcgg gtgatttcgc gggctttgcg ggcggcttcg cgcgcgcggg
     2941 cggcatcgac gattttgccg gtgatgattt tggcttcgtt cgggttttct tcaaggaagt
     3001 cggtcagcgc ttggttgatg acttcgttga caacggggcc gatttcgctg gaaaccagtt
     3061 tgtctttggt ttgggatgag aatttggggt cgggcagttt gacggacaac acgcaggtca
     3121 aaccttcgcg catatcgtcg cctgctgttt ccactttggc ttttttggcg acttcgttgg
     3181 cttcgatgta gctgttgatg gtgcgtgtca tcacttggcg caacgcggtc aagtgcgtac
     3241 cgccgtcgcg ctgagggatg ttgttggtga agcactgcac gctttcttgg tagctgtcgt
     3301 tccactgcat cgcgcattcg acgctcatgc cgtctttctc gccgaacgca tagaagattt
     3361 tttcgtgcaa cggcgttttt ttgcggttca tgtattgcac gaaaccggcc acgccgccgg
     3421 aaagggcgaa gctttcgtgt ttgccgtcgc gttcgtcggt caattcgatg tccacgccgt
     3481 tgttcaggaa ggaaagttcg cgaatgcgtt tggcgaggat gtcgaagctg tattcgacgt
     3541 tgccgaaagt ttccgtgctg gcgaggaagc gtacggtcgt gcctttcttg tcggaatcgc
     3601 cgacaacttt cagcggctct tcggtttcgc cgcgcacgaa gcggacgaag tgttctttgc
     3661 cgtcgcggta gatggtcagc gtaacccagt cggacagcgc gttgacgacg gacacgccca
     3721 cgccgtgcag gccgccggag attttgtagc tgttgttgtc gaatttaccg cccgcgtgca
     3781 agacggtcat gatgacttcg gcggcggagc gtccttcttt cgggtggatg ccggtgggca
     3841 taccgcgccc attgtcggcg acgctgacgg aatggtcggc gtggatggta acggtgattt
     3901 tgtcgcaatg tccggcgagt gcttcgtcaa tggcgttgtc caatacttcg aacaccatgt
     3961 ggtgcaggcc gctgccgtcc tgcgtgtcgc cgatgtacat accggggcgt ttgcgtaccg
     4021 cttccaagcc ttcgagcacc tgaatgctgt cggcgccgta ttcttcgtgt ttttgttcag
     4081 tcatattttt tgccggattt tgaaaagatt ttgcgatgcc gccaaaacaa gtccgcacct
     4141 tgtggaaaaa gcgggcggga cgacatatat aattgtgtat tatagccgat tttgccgcct
     4201 aattcagcgt tatccgcatc agtgtgccgc cgggaaaaga tgaaacggta cgtttgcctc
     4261 cggcatcagg tcggggattg tcccgtaaag tggcaaaagc gtttttttgc cactaaaatc
     4321 tacaccctat acttttcgga caggggcgcg gaaatggaaa tatggaatat gttggacact
     4381 tggctcggtg ccgtcccgat acgtgcggag gcggtcgaat ccgtggcggt ggtcgcggct
     4441 ttgctgctgg cgcgcgccct tctgttgaat atccacttca aacggcatcc ggatttcggc
     4501 atcgaaagca agcggcggtt tttggttgcc agccgcaata taacgctgct tttggtgctg
     4561 ttttcgctgg catttatctg gtcggcgcaa atccaaacgc tggctttgtc gatgtttgcg
     4621 gtggcggcgg cggtcgtcgt ggcgacgaag gaactgatta tgtgtctgtc gggcagtatt
     4681 ttaaggtctg ccacccagca atactcggtc ggcgactata tcgaaatcaa cggcctgcgc
     4741 gggcgcgtgg tcgacatcaa cctgttgaac acgctgatga tgcaggtcgg tccgaacccc
     4801 ttggtcggac agcttgcggg aaccaccgtt tctttcccca acagcctgtt gttgagccac
     4861 cccgtgcgcc gcgacaatat tttgggcgac tatgtcatcc atacggtcga aatccccgtt
     4921 cccatccatt tggattcgga tgaagccgta tgccgtctga aagccgtact cgagcccttg
     4981 tgcgcgccct acatccccgc catccaacgg catttggaaa acgtgcaggc ggaaaaactg
     5041 tttatcacgc ccgccgccag accgcgcgtt acccgcgtgc cgtacgatga caaggcatac
     5101 cgcatcatcg tccgcttcgc ctcccccgtt tcaaagcggc tggaaatcca acaggcggtt
     5161 atggacgaat ttttgcgcgt acaataccgc ctgttaaatt accccgccgg ctccgaaaca
     5221 ctttaacttt ccccgaccga ccccatttcc ggcttcagac ggcatattgc cgatatgctg
     5281 tctgaagcac aacacgcaaa ggaaacccat cttatgactg acaacgcact gctccatttg
     5341 ggcgaagaac cccgttttga tcaaatcaaa accgaagaca tcaaacccgc cctgcaaacc
     5401 gccatcgccg aagcgcgcga acaaatcgcc gccatcaaag cccaaacgca caccggctgg
     5461 gcaaacactg tcgaacccct gaccggcatc accgaacgcg tcggcaggat ttggggcgtg
     5521 gtgtcgcacc tcaactccgt cgccgacacg cccgaactgc gcgccgtcta taacgaactg
     5581 atgcccgaaa tcaccgtctt cttcaccgaa atcggacaag acatcgagct gtacaaccgc
     5641 ttcaaaacca tcaaaaactc ccccgagttc gacaccctct cccccgcgca aaaaaccaaa
     5701 ctcaaccacg atctgcgcga tttcgtcctc agcggcgcgg aactgccgcc cgaacagcag
     5761 gcagaactgg caaaactgca aaccgaaggc gcgcaacttt ccgccaaatt ctcccaaaac
     5821 gtcctagacg cgaccgacgc gttcggcatt tactttgacg atgctgcacc gcttgccggc
     5881 attcccgaag acgcgctcgc catgtttgcc gctgccgcgc aaagcgaagg caaaacaggc
     5941 tacaaaatcg gtttgcagat tccgcactac ctcgccgtca tccaatacgc cgacaaccgc
     6001 gaactgcgcg aacaaatcta ccgcgcctac gttacccgcg ccagcgaact ttcagacggc
     6061 ggcaaattcg acaacaccgc caacatcgac cgcacgctcg caaacgccct gcaaaccgcc
     6121 aaactgctcg gcttcaaaaa ctacgccgaa ttgtcgctgg caaccaaaat ggcggacacg
     6181 cccgaacaag ttttaaactt cctgcacgac ctcgcccgcc gcgccaaacc ctacgccgaa
     6241 aaagacctcg ccgaagtcaa agccttcgcc cgcgaaagcc tgaacctcgc cgatttgcaa
     6301 ccgtgggact tgggctacgc cagcgaaaaa ctgcgcgaag ccaaatacgc gttcagcgaa
     6361 accgaagtca aaaaatactt ccccgtcggc aaagtattaa acggactgtt cgcccaaatc
     6421 aaaaaactct acggcatcgg atttaccgaa aaaaccgtcc ccgtctggca caaagacgtg
     6481 cgctattttg aattgcaaca aaacggcgaa accataggcg gcgtttatat ggatttgtac
     6541 gcacgcgaag gcaaacgcgg cggcgcgtgg atgaacgact acaaaggtcg ccgccgtttt
     6601 tcagacggca cgctgcaact gcccaccgcc tacctcgtct gcaacttcgc cccgcccgtc
     6661 ggcggcagag aagcgcgttt gagccacgac gaaatcctca tcctcttcca cgaaaccgga
     6721 cacgggctgc accacctgct tacccaagtg gacgaactgg gcgtgtccgg tatcaacggc
     6781 gtagaatggg acgcggtcga actgcccagc cagtttatgg aaaatttcgt ttgggaatac
     6841 aatgtattgg cgcaaatgtc cacccacgaa gaaaccggcg agcccctgcc gaaagaactc
     6901 ttcgacaaaa tgctcgccgc caaaaacttc caacgcggaa tgttcctcgt ccgccagatg
     6961 gagttcgccc tcttcgatat gatgatttac agcgaagacg acgaaggccg tctgaaaaac
     7021 tggcaacagg ttttagacag cgtgcgcaaa gaagtcgccg tcatccaacc gcccgaatac
     7081 aaccgcttcg ccttgagctt cggccacatc ttcgcaggcg gctattccgc aggctattac
     7141 agctacgcgt gggcggaagt attgagcgcg gacgcatacg ccgcctttga agaaagcgac
     7201 gatgtcgccg ccacaggcaa acgcttttgg caggaaatcc tcgccgtcgg cggatcgcgc
     7261 agcgcggcag aatccttcaa agccttccgc ggccgcgaac cgagcataga cgcactcttg
     7321 cgccacagcg gtttcgacaa cgcggcctga cggcaagggt gaagtaaaaa ataggaggat
     7381 tcgatataaa aacatccgca ccgtcattcc cgcgtaggcg ggaatccaga ccggtcggca
     7441 cggaaactta tcggataaaa cggtttcttt agattttacg ttctagattc ccgctttcgc
     7501 gggaatgacg cggaaaagtt gctgtgattt cggataaatt ttcgcaacgt ttaatttccg
     7561 ttttacctga taaatgcccg caatctcaaa tcccgtcatt ccccaaaaac aaaaaaaatc
     7621 aaaaacagaa atcccgtcat tcccgcgcag gcgggaatcc aggtctgtcg gtgcggaaac
     7681 ttatcggata aaacggtttc tttagatttt acgttctaga ttcccgcttt cgcgggaatg
     7741 acggaatatt tttgaatttg ataaaaatgc cgtctgaaac ggtcaaacaa cgcttcagac
     7801 g
//
LOCUS       DAXHPG010000098         7634 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXHPG010000098 DAXHPG010000000
VERSION     DAXHPG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.098"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(32..448)
                     /gene="dksA"
                     /locus_tag="ACFM6F_001478"
     CDS             complement(32..448)
                     /gene="dksA"
                     /locus_tag="ACFM6F_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC6164874.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(595..1404)
                     /gene="proC"
                     /locus_tag="ACFM6F_001479"
     CDS             complement(595..1404)
                     /gene="proC"
                     /locus_tag="ACFM6F_001479"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC6164875.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLQKELGVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVDTL
                     SRYLGGTRRIVRVMPNTPGKIGLGVSGMFAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1435..1842)
                     /locus_tag="ACFM6F_001480"
     CDS             complement(1435..1842)
                     /locus_tag="ACFM6F_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC6164876.1"
                     /translation="MGMRTIKYLAMALLLMFTVAGCQLAGWYPCPSLSGWCMPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSECVRW"
     gene            complement(1824..2528)
                     /locus_tag="ACFM6F_001481"
     CDS             complement(1824..2528)
                     /locus_tag="ACFM6F_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC6164877.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRDPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTIC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVVLAVEVAKLPNIVVRG
                     LMCVAKAGGSEAELKTQFQTMQKLLADLNTAGVKADVLSMGMSDDMPAAIACGATHVR
                     IGSAIFGKRSRLECVQ"
     gene            2648..3691
                     /gene="pilT"
                     /locus_tag="ACFM6F_001482"
     CDS             2648..3691
                     /gene="pilT"
                     /locus_tag="ACFM6F_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC6164878.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3854..5080
                     /locus_tag="ACFM6F_001483"
     CDS             3854..5080
                     /locus_tag="ACFM6F_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6164879.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEISAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5366..7516
                     /gene="yccS"
                     /locus_tag="ACFM6F_001484"
     CDS             5366..7516
                     /gene="yccS"
                     /locus_tag="ACFM6F_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC6164880.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIITTVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDNPDIRHLRRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1883 a         2536 c         1665 g         1550 t
ORIGIN      
        1 ggggattcat acggcatttt gtttgccgcc atcagtcggc aaactgtttt ttcatcctct
       61 ctcggcgttc ttgggactca acagataaag tggctgtcgg gcgtgccagc agccgcttca
      121 gaccgatagg ctctcccgta tcggcacaga atccataatc cccttcatca atattgcgga
      181 tggtcgcctg tattttactg agaagttttc gttcccgatc gcgggtacgg agttccaatg
      241 cgtactcttc ttcctgtgtg gcacggtcgg caggatcggg ggctgattcg tgttcttgga
      301 gatgccctgt cgtagcggaa gcattttcga tgagttcgtc ttgcattttt accagcaatt
      361 cgcggaaaaa agccaaatgc tcgtcattca tataatcgtc ttccggcccg ttccaattca
      421 aaatatcttg ttctgtcagc tttgccataa tgtatatcct ctcggttcac cgaaaaaaac
      481 aaagcaccac aaagtggctt tattcctttg atttttataa ggcttattgc ctagggcgtt
      541 tttgaaaacg cgcatcataa cacgttttgg attggttttt ttattttctt tacattattg
      601 atattgccgt tccatttcct gcgaacggcg cacacaggca caaacgccct cgcttatggc
      661 ttcggcgaca cgatgccgcc tgaaagcttc cacggcttcg tgggttgtcc cgccttttga
      721 cgttacattt ttttgaagct tctcgaaatc ttcacccgtc tgctcggcaa gggcaaccgc
      781 tcctttaaac gttgccagac tgagcgcgcg tgcttctgcc atatcaaacc cttgtcggat
      841 ggcagcattt tgcaatgcgt ccagcagata aaacacataa gccggtccgc tgccgctgat
      901 accggtaatg ctgtgcattt gcgcttcatc ttccaaccaa acggtcaaac cgactgattt
      961 catgattcga tcggcaatcc tgcggtctgt ttccgatact tccgcttcgg caaacatacc
     1021 ggatacgccc agcccgattt ttccgggtgt attcggcata acccggacaa tgcggcgtgt
     1081 tcccccgagg taacggctga gcgtatcaac cgacaatcca gctgcaacag aaagcaccaa
     1141 tgcgccgttg gtgcggatat ttttgcacgc ggcttccata tcctgcggct tgacggcaag
     1201 gattaaaaca tcgtcggaat gaagctccgg caaggtttca gaagtttcga cccccaactc
     1261 tttttgcaaa cgttcgcgct tttccgcacc ccggttggca atatggatgc ggtaaccgcc
     1321 ttgtttgacc aatccgcccg caacggcagc cgccatattg ccgccgccga gaaaataaac
     1381 attcattatt tctaccctat tcaaaattac cttactaaat tcattcccaa ttttttacca
     1441 tcggacacac tcggacagac cgttgcgccg caacccctgc tttttcaaac aatcaaaacc
     1501 ttcctgtttg aacgtctcaa tcaaaggttt gccgtcacgc gtacgccaat ccaacccgca
     1561 ggcttcaaac ttccctattt tcctgtaaaa gtaagattgt tgtgcggatt catattcgtt
     1621 tgccctaacg gaacgattgc cgtctgaaag cggtatctcg tagtcctcta aagacggcgg
     1681 actctcgccg ccaatatccc aaaaatcgat ggcagccggt tttctcggca tacaccagcc
     1741 ggacaacgac ggacatggat accaacccgc caactgacag ccggcaaccg taaacataag
     1801 caataacgcc attgcaagat attttattgt acgcattcca atctgctcct tttcccgaaa
     1861 atcgcactgc cgatacggac atgtgttgca ccgcacgcaa tggcggcagg catatcgtcc
     1921 gacatcccca tagacagcac atccgcctta acgccagccg tattgaggtc ggcaagcaac
     1981 ttttgcatgg tctgaaactg ggttttcaat tctgcctcgc tgccgccggc tttggcaaca
     2041 cacatcagtc cgcgtacgac gatattcggc aacttcgcca cttcgaccgc aagcacgacc
     2101 gcttcttcgg gcgcgacacc gtgtttcgcc gcctcgcccg caatgttcac ctcgatacac
     2161 acctgcaaag gcggcattga ggaaggacgt tgcccgctca gccggacagc ggttttcaga
     2221 cggcatatgg tatgcaccca atgcgcgcgt tcggcgacaa acttggtttt gttggactgc
     2281 acatcgccga tgacgtgcca cacgatgtcg gtcaaatccg ccaactcttc cgttttgccg
     2341 taccactcct gaatatagtt ctcgccgaaa tcacgctgtc cggcggcgta aacttcgcgg
     2401 atgccgtctg aagggaaagt cttaccgacg gcaatcaggc tgacggaatg cggatccctg
     2461 cccgcctgca gaaccaattt tccgatacgg tcggacacct cacaataacg ttcttgcaac
     2521 accgtcatag attatcccct aattaaaaat gattaaacag ttgaaacccc tccagtcagg
     2581 gcggtacaat caaggttgtt agaaccattc caaccaatcg aaacattata ctaaacagag
     2641 ccgcattatg cagattaccg acttactcgc cttcggcgct aaaaacaaag catccgacct
     2701 tcacctgagt tcgggcatat cccctatgat tcgggttcac ggcgatatgc ggcgcatcaa
     2761 ccttcccgaa atgagcgcgg aagaggtcgg taatatggta acttcggtga tgaacgacca
     2821 ccagcggaaa atctaccagc aaaacttgga agtcgacttc tcgttcgaac tgcccaacgt
     2881 cgcccgattc cgcgtcaacg ccttcaacac cggccgcggt cccgccgccg tattccgcac
     2941 cattcccagc accgtcttat cgctggaaga attgaaagcc ccgagcattt tccaaaaaat
     3001 cgcagaatcg ccgcgcggca tggtattggt taccggccct accggttcgg gcaaatcgac
     3061 cacgcttgcc gcgatgatca actacatcaa cgaaacccag ccggcacaca tcctgaccat
     3121 cgaagacccg atcgaattcg tccaccaaag caaaaaatcc ctgattaacc agcgcgagct
     3181 gcaccagcac accctcagct tcgccaacgc gctgcgttcc gcattgcgcg aagaccccga
     3241 cgttatcctt gtcggcgaga tgcgcgaccc cgaaaccatc ggcttggcac tgaccgccgc
     3301 cgaaaccgga cacttggttt tcggcacgct gcacacgacc ggcgcagcaa aaaccgtcga
     3361 ccgtatcgtg gacgtattcc cggcgggaga aaaagaaatg gtgcgctcta tgctgtccga
     3421 atcgctgacc gccgtcatct cccaaaacct gctgaaaacg cacgacggca acggccgtgt
     3481 cgcctcgcac gaaatcctga ttgccaaccc cgccgtccgc aacctcatcc gcgaaaacaa
     3541 aatcacgcag attaactccg tcctgcaaac cgggcaggcg agcggtatgc agacaatgga
     3601 ccaatcgctg caatcgctgg tgcgccaagg gctgatcgca ccggaagtcg cacgcagacg
     3661 cgcgcaaaac agcgaaagta tgagtttctg acacacaacc gccttccggc cataccggcg
     3721 ggaaaacaag gcgcaaacac gcggggcggg acgcagcatc ccgcccggct acctttccga
     3781 acaagaagcg tccgccttcc tgttgaaacc tgccgccgca aactgcaagg cttaaaccga
     3841 aaagaagcta acgatgaata ccgataacct gcacgacatc ttggacgaaa tggttcaagt
     3901 gtattctcaa aaaaaacaaa gccgatccga aaccccggcc gaaatcagcg cacacttcca
     3961 cccgctgctc gaccgcctgt gcgaaaccgc agaagcacaa aacgcgtccg acatccttat
     4021 cagcaaagga ttcccgccct cgttgaaaat caacagcgca ttaaccccgc agccgcaaaa
     4081 ggcgctgacg ggcgaggaaa ccgccgccat cgccgcatcg acgatgaacg ccgaacaatc
     4141 ggaaatattc cggcgcgacg gcgaaatcaa ctactccgtc cagtcgcgca gcggcacgcg
     4201 ctaccgcgcc aacgcctacc acagccaagg cagcgcaggt ttggttttgc ggcgcatcaa
     4261 ccacgtcatc ccgcaaatgc aggaattggg cctgcccgaa aaactcaaag acctcgccgt
     4321 cgcaccgcgc gggctgctga ttatcgtcgg gcctaccggt tcgggcaaat ccaccacgat
     4381 ggcgactatg ctcgaacacc gcaacaaaac cctgcccagc catatcgtta ccatcgaaga
     4441 cccgattgag tttatctaca aaccgcgccg ctgcatcttt acccagcgcg aaatcggcgt
     4501 cgacaccata aactggcaga cggcggtaca aaacgctatg cgccaatccc ccgacgtggt
     4561 ctgcatcggc gaagtccgca gcagggaaag tatggaatac gcgatgcagc tcgcccaaac
     4621 cggccacctg tgcattttta cgctccacgc caacaccgcg ccgcagtcgc tcgaacgcat
     4681 actcaacttc taccccaaag aacagcacaa ccaaatactg atcgacatcg ccctcaacct
     4741 gaccggcatc atctgccaac gcctcgccct caaacaagac aaaacgggca ggacggcggt
     4801 tgtcgacttg ctcatcaaca cgcccgccat ccaagacttc atcctgaagg gcgacctgat
     4861 gaacatcagt aaaatcatgg aaaccgccaa aaccgacgga atgcagacga tggatcaaaa
     4921 ccttttcgaa ctgtaccgtc acggcatcat cagttacgaa gaagccctgc gccagtccgt
     4981 ttccgccaac aacctgcgat tgcacatcca actgcacaaa gaaggcaaaa cgcccgaact
     5041 cctttacgac agggtcaacg gtctcaacct catttcctga tccgcaaaac ccaatgccgt
     5101 ctgaaaaccg catccccgtt ttcagacggc atgattttat ccgccccatt catgtgctac
     5161 actttatagt ggattaaatt tatagtggat taacaaaaat caggacaaga cgacgaagcc
     5221 gcagacagta caaatagtac ggaaccgatt cacttggtgc ttcagcacct tagagaatcg
     5281 ttctctttca gctaaggcga ggcaacgccg tactggtttt tgttaatcca ctatattcac
     5341 ttcataacaa taaaccggta aaaccatgaa aaccccactc ctcaagcctc tgctcattac
     5401 ctcgcttccc gttttcgcca gtgtttttac cgccgcctcc atcgtctggc agctaggcga
     5461 acccaagctc gccatgccct tcgtactcgg catcatcgcc ggcggccttg tcgatttgga
     5521 caaccgcctg accggacggc tgaaaaacat catcaccacc gtcgccctgt tcaccctctc
     5581 ctcgctcacg gcacaaagca ccctcggcac agggctgccc ttcatcctcg ccatgaccct
     5641 gatgaccttc ggctttacca ttttaggcgc ggtcgggctg aaataccgca ccttcgcctt
     5701 cggcgcactc gccgtcgcca cttacaccac acttacctac acccccgaaa cctactggct
     5761 gaccaacccc ttcatgattt tatgcggcac cgtactgtac agcaccgcca tcatcctgtt
     5821 ccaaatcatc ctgccccacc gccccgtcca agaaagcgtc gccaacgcct acgacgcact
     5881 cggtggctac ctcgaagcca aagccgactt cttcgacccc gatgaggcag cctggatagg
     5941 caaccgccac atcgacctcg ccatgagcaa caccggcgtc atcaccgcct tcaaccaatg
     6001 ccgttccgcc ctgttttacc gccttcgcgg caaacaccgc cacccgcgca ccgccaaaat
     6061 gctgcgctac tacttcgccg cccaagacat acacgaacgt atcagctccg cccacgtcga
     6121 ctaccaagag atgtccgaaa aattcaaaaa caccgacatc atcttccgca tccaccgcct
     6181 gctcgaaatg cagggacaag cctgccgcaa caccgcccaa gccctgcgcg caagcaaaga
     6241 ctacgtttac agcaaacgcc tcggccgcgc catcgaaggc tgccgccagt cgctgcgcct
     6301 cctttcagac agcaacgaca atcccgacat ccgccacctg cgccgccttc tcgacaacct
     6361 cggcagcgtc gaccagcagt tccgccaact ccgacacagc gactcccccg ccgaaaacga
     6421 ccgcatgggc gacacccgca tcgccgccct cgaaaccggc agcctcaaaa acacctggca
     6481 ggcaatccgt ccgcagctaa acctcgaatc aggcgtattc cgccatgccg tccgcctgtc
     6541 ccttgtcgtt gccgccgcct gcaccatcgt cgaagccctc aacctcaacc tcggctactg
     6601 gatactgctg accgcccttt tcgtctgcca gcctaactac accgccacca aaagccgcgt
     6661 ccgccagcgc attgccggta ccgtactcgg cgtaatcgtc ggctcgctcg tcccctactt
     6721 taccccctcc gtcgaaacca aactctggat cgtcatcgcc agtaccaccc tctttttcat
     6781 gacccgcacc tacaaataca gtttctcgac atttttcatc accattcagg cactgaccag
     6841 cctctccctc gcaggtttgg acgtatacgc cgccatgccc gtgcgcatca tcgacaccat
     6901 tatcggcgca tcccttgcct gggcggcagt cagctacctg tggccagact ggaaatacct
     6961 cacgctcgaa cgcaccgccg cccttgccgt atgcagcaac ggtgcctatc tcgaaaaaat
     7021 caccgaacgc ctcaaaagcg gcgaaaccgg cgacgacgtc gaataccgcg ccacccgccg
     7081 ccgcgcccac gaacacaccg ccgccctcag cagcaccctt tccgacatga gcagcgaacc
     7141 cgcaaaattc gccgacagcc tgcaacccgg ctttaccctg ctcaaaaccg gctacgccct
     7201 gaccggctac atctccgccc tcggcgcata ccgcagcgaa atgcacgaag aatgcagccc
     7261 cgactttacc gcacagttcc acctcgccgc cgaacacacc gcccacatct tccaacacct
     7321 gcccgaaacc gaacccgacg actttcagac agcactggat acactgcgcg gcgaactcga
     7381 caccctccgc acccacagca gcggaacaca aagccacatc ctcctccaac agctccaact
     7441 catcgcccgg cagctcgaac cctactaccg cgcctaccga caaattccgc acaggcagcc
     7501 ccaaaacgca gcctgaaaaa gtttcggcat tttgtaagag agagcagatt gtcagatagg
     7561 ttgcaagata gtggataaag ctttgcgcag ggtaaatgcg tagcaactga accgtcattc
     7621 ccacgaacct acat
//
LOCUS       DAXHPG010000099         7584 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXHPG010000099 DAXHPG010000000
VERSION     DAXHPG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.099"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(52..360)
                     /locus_tag="ACFM6F_001485"
     CDS             complement(52..360)
                     /locus_tag="ACFM6F_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164881.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(344..646)
                     /locus_tag="ACFM6F_001486"
     CDS             complement(344..646)
                     /locus_tag="ACFM6F_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC6164882.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(720..3083)
                     /gene="pheT"
                     /locus_tag="ACFM6F_001487"
     CDS             complement(720..3083)
                     /gene="pheT"
                     /locus_tag="ACFM6F_001487"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC6164883.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAMERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAAHGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3108..3758)
                     /locus_tag="ACFM6F_001488"
     CDS             complement(3108..3758)
                     /locus_tag="ACFM6F_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFC6164884.1"
                     /translation="MITISNEDNMILMSRYPDKYFDLAIVDPPYGILNKTKRGGDYKF
                     NMNEYSQWDIKPDQTYFNELFRVSKNQIIWGGNYFGELWLRSEYNKGFIIWDKNQPET
                     LNNFSMAEMAWSSFDRPSKIFRFSVRKNRNKTHPTQKPVELYQWLLKMYAKQGDKILD
                     THLGSGTLAIACCIAQFDLTACEINSDYYQQSIEKIKNNLPEARISFGHPGYCIIE"
     gene            complement(3755..5224)
                     /locus_tag="ACFM6F_001489"
     CDS             complement(3755..5224)
                     /locus_tag="ACFM6F_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6164885.1"
                     /translation="MTTARWVIDKHLQNFHILCKFAGILKTNSFISVEDKAKLSEKLE
                     KLDLYHRRNTGKSLDATTHKIKELSFYMFGYRDVCGQVTQKFLFSPLGNLFLKHLDNN
                     EYIQKIFLTMLWAIPFPHPYIKTDESIQLYPMRLIFKLLSDERLDCKLFSYEYIYLIS
                     FVKSADQNSYEKLVQDILVLRTCAEVKIKHQLTAENSRSHAYVNAAHEWESYFSKTLT
                     DAGVLQKTDGKIICRLKHGKTETYRKVTSSEFSIPKQLQEFVKKLQSAYSFSEMPLNL
                     NDSDRLKIDVIKEIYSFYPKELLEEIGELKDEAAYELLHLPRLIEQYADNNNGTEAYL
                     FEDVLEMGFNMFYNVEAKKIGGPGNTDLECLYITQKRKFAVEAKSTKNKLSGINSGRL
                     EDHKNKIKAIYTIVVTPRYVPAVLSDIRNCPIVIIRANTFAEFLYNCLINRSSIPEID
                     YRYFDEIIIKNLGKDISSEISNLTMQQFASNTTMEAYST"
     gene            complement(5227..6285)
                     /locus_tag="ACFM6F_001490"
     CDS             complement(5227..6285)
                     /locus_tag="ACFM6F_001490"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6164886.1"
                     /translation="MMIGASLFSSAGIAETYLHNAGIKIIAANELVPERANLYKALYP
                     ESKMIIGDILHEEVFQNLIQSVPNRLDFLIASPPCQGMSVAGKNRNIQEMANDKRNHL
                     IFRVIEFILLKRPTFVLIENVPFFLKIKLPYKGTLQTVEVILQDLFGCEYYIQTHIFD
                     SADYGVAQHRKRAIIRMNKHSTIWGMPEKVTKTISVRDAISFLPSIESGQKSNVKWHF
                     ARTHAPEHIIWLKNTPTGRSAFDNIEHYPKKKNGEKIKSYNTTYRRMEWDAPAPTITI
                     RNDAISSQLNVHPGRSMPDGTYSDARVLTPLELMLLTSLPIDWNIPDDTSELLIRQCI
                     GESIPPLLIKKIVERIGK"
     gene            complement(6468..7460)
                     /gene="pheS"
                     /locus_tag="ACFM6F_001491"
     CDS             complement(6468..7460)
                     /gene="pheS"
                     /locus_tag="ACFM6F_001491"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC6164887.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
BASE COUNT         2071 a         1802 c         1492 g         2219 t
ORIGIN      
        1 ttttttagcg tgtaaaacaa aaccggctgc atacctgcaa ccggcctcaa atcagcacaa
       61 ttccttatcc aaatccgcca acagctcttt cagaccgtct ccgatttcct gaagcgtaac
      121 cggacggttg ccgtccgaac ccgattccgc accttctgca tacggtgcaa acgtgctctg
      181 caatttcaac agtttcacca catccaaacg ggtgtagcgt tcgccgccgt aaccgacaac
      241 cacaccgtga tcatgctgcc attgcgcaaa accatagggg ctgatttgca acagtccgca
      301 caactcgtcc agcgtgaaat agcgttttgc gggaaccttc aaatcaacgt tgtttgtcat
      361 agtagtgttc caccatgctt ttgagtttct ggctggcatg gaaagttacc acgcggcggg
      421 cggtaatcgg cacttcctcg ccggttttag ggttacgacc cgggcgttgc ggcttgtcgc
      481 gcaactggaa atttccgaaa ccggaaattt tgatttcttc gccgcttgcc aaagtgctgc
      541 ggatttcttc aaaaaagagt tcgacgattt ctttggcatc gtttttggtg acgttgctga
      601 ctttgtctac caaaatatcg gccagttctg ctttagtgag agtcatatga ttaccttctg
      661 ttgtaacagt tgtggattat taccaatttt tatttaaaat tcaagcgaat atttattttt
      721 taactgcgaa gccgcgcccc tgccgccgtt gccgcgccaa tcagttttcc gataagcggc
      781 tcgaccgcct catccgtcag cgtgttttcc atatcctgca aaatcacttt gaccgccacg
      841 ctcttcatcc cttcgggcag tcccgtgccg cgatacacgt caaacacgct gatttcctgt
      901 accaacttgt ttgccgcgcc tttcaagaca agcagcaaat catcatggct catagcttcc
      961 ggcatcacaa acgccaaatc gcggcgcacc ggctggaatt tcgatacggc ccgatagcgc
     1021 gttttcccgc attccaacac ggccgccata tcgatttcaa ataccagcgg cgcttgcggc
     1081 aggtcgtatt tttgcagcca tttcggatgc agttcgccga caaagccgat gactttgccg
     1141 tctgaaacga tattggcggc acgtccggga tgcagggcgg gatgtccggt tttaacgaac
     1201 tcgactgctt tgtttttcaa cagattttcc acatctgcct taatgtcgta aaaatccgca
     1261 ttgcgcgttt tcccacccca ttgttcgggc attgccgcgc cgtaccacag accgccgatg
     1321 cgttcgtttt ggacaaaccg gccgtctgaa cctttgccga acacgcgggc gatttcaaac
     1381 acgcgcacgc ggttttgttt gcggttcagg ttgttttgca aaatttccac caagccgccg
     1441 atgagtgtgg aacgcatcac ggcatattgc gccgccagcg ggttttgcag gcggatgggg
     1501 tcggcgttgg cggcaaaatc ttgttcccac tgctcgtcaa cgaaggcata gctgaccact
     1561 tcgcggtagc cgtgcgccgc catttcgttg taaacggcaa aacgcgggcg gcgtgtttcg
     1621 ggcagttcca gcattttcag acggcctgac gtgtaatcgt cggggatgtt ttcatagccg
     1681 taaacgcgtc cgatttcttc aatcaaatcg gcctcaattt cgatgtcaaa acggaagctc
     1741 ggcgcggtaa cgcggaagcc ttccgccgtt ttctcgggct gcaggcccaa gtgttgcaaa
     1801 atggtttcca cctgttcggc aggaatgtcc acgcccaaca cggttttcag acggtccaaa
     1861 cgcagtccga cctgcttggc ttcgggcaat ttaccttgtg cttcaaccat ctcgcctgcc
     1921 gcaccgccgc aaatctgcaa caccaattcg gtagcacgtt ccatggcatc cgcctgcaaa
     1981 cggtaatcca cgccgcgctc gaagcggaac gacgaatccg aaccgaaacc gtattggcgc
     2041 gatttgccgg cgatgatttc gggcgcaaac caagccgctt ccagcacgat attttgcgtg
     2101 ccgtctgaaa ccgcgctcgc cgcgccgccc attaagcccg ccaaactcaa cacgcctttt
     2161 tcgtccgcca cgaccagcgt gttttcagac agggaaacgg ttttctcgtt caggcattcc
     2221 agcgtttccc cttcgcgcgc gcggcggatg tgcaggctgc cggaaagttt gtcggcatca
     2281 aaaacgtgca tcggctgacc gatttccagc atcacataat tgccgatgtc caccagcgcg
     2341 gaaatactgc ggatgccgct gcgctccaaa cgttgtttca tccaatcggg agtagccgct
     2401 ttcgcattga cattttcaat aacgcggctg ataaaacggc cgcaatcggc aggcgcgtta
     2461 atctgcacgg gctgttttcg actgcccgtg atcggcgcgg tatggatttc gggctgcctg
     2521 aacgcgcacc ccgtcaatgc ggacacttcg cgcgcaatgc ctttgatgct caagcagtcg
     2581 gcgcggttag gcgtaatttt caacgtaaac agcgtatcgt ccaaatccaa gtattcgcgg
     2641 atattggtac cgacgggcgc atcttcaggc agaatgtgca ggccgtctac accatcatca
     2701 ggcaaaccta gttcattggt cgaacacagc ataccgtttg atggcacacc gcgcattttg
     2761 gtcggcttga ttttgaaatt acccggcaaa acggcacccg gcagcgaaca cggcactttg
     2821 atgcccgctt tcacattcgg cgcaccgcac acaatctgca ccaacccgcc cgtacccgca
     2881 tcaactcggg taacgttcaa acggtctgca tccggatgtt tttcaacgga tttcacttcg
     2941 gcaatcacca cgcccgcaaa cgcaggcgcg gcagtttcag cctcttccac ttccaagccg
     3001 gacatcgtta acagatgttc cagcttatcg gaggaaagtt cggtatcggc ttgggttttc
     3061 agccatgagt aggagaattg catggttaat ttctctatat ttttaagtta ttcaataata
     3121 caataacctg gatgcccaaa actgattcta gcttcaggta aattattttt tattttctca
     3181 atcgattgtt ggtaataatc ggaattgatt tcacaagctg tcaaatcaaa ctgtgcaatg
     3241 cagcatgcaa tagcaagagt tccacttcct aaatgtgtat ctaaaatctt atcaccctgc
     3301 tttgcataca tttttaacaa ccactgatat aattcgactg gtttttgtgt tgggtgagtt
     3361 ttattacgat ttttccgcac actaaaccgg aaaattttag atggcctatc gaatgacgac
     3421 caagccattt ccgccataga aaaattattt aatgtctctg gttgattctt atcccaaata
     3481 ataaatcctt tattatattc acttctcaac cataactcgc caaaataatt cccaccccaa
     3541 ataatttgat tttttgacac gcgaaataat tcattaaagt aagtttggtc tggcttaata
     3601 tcccattgtg agtattcatt catattgaat ttataatcac caccacgttt agttttattc
     3661 aaaatcccat aaggaggatc tacaattgcc aaatcaaaat acttgtcagg ataccgagac
     3721 attaagatca tgttatcttc atttgaaata gttatcatgt actatacgct tccattgtgg
     3781 tgttacttgc aaactgttgc atagtcaaat tggaaatttc tgaactaata tcttttccaa
     3841 gatttttaat aataatttca tcaaaatacc gataatcaat ctctggaata ctggagcgat
     3901 taatcaaaca attatataaa aattcagcaa atgtattggc acgaataatt acaattgggc
     3961 aattacgaat atcggataat acggcaggga cataacgtgg tgtgacaaca attgtgtaaa
     4021 tggccttaat tttattttta tgatcttcca atcttcctga attaatacct gataacttat
     4081 ttttagttga ttttgcctcc actgcaaatt ttctcttttg cgtaatatat aagcactcta
     4141 aatccgtatt acctggtcca ccaatttttt tagcttctac gttataaaac atattgaacc
     4201 ccatttctag aacatcttca aatagatatg cctctgttcc attattatta tctgcatatt
     4261 gttcaatcaa cctaggtaag tgcaataatt catatgctgc ttcatcctta agctcaccaa
     4321 tttcctccaa taactctttt ggatagaagc tataaatttc cttaatgaca tcaattttca
     4381 aacgatcact atcgttcaga tttaatggca tttctgaaaa cgaataagca ctttgcaatt
     4441 ttttcacaaa ttcctgaagt tgcttaggaa tcgaaaactc acttgatgtt actttacgat
     4501 atgtttcggt cttaccatgc tttagacggc aaataatttt accatctgtt ttttgcaaaa
     4561 cacctgcatc agtcaatgtt tttgaaaaat aagattccca ctcatgtgct gcatttacat
     4621 aagcatgact acgactattt tccgcagtta attgatgttt aatttttact tcagcacatg
     4681 ttcgtaacac caaaatgtct tgtactaatt tttcatagct attctgatca gcagatttca
     4741 caaatgaaat taaatagata tattcataag aaaatagttt acaatccaat ctttcatcag
     4801 ataacaactt aaatattagt ctcatgggat ataattgaat actttcatca gtcttaatgt
     4861 acggatgagg aaatggtatc gcccacaaca tagtaagaaa aattttttga atatattcat
     4921 tattatccaa gtgtttcaaa aataaattac ccaatggact gaacaggaat ttctgtgtaa
     4981 cttgcccaca cacatcacga taaccaaaca tatagaatga taattctttt attttatgag
     5041 tagtagcatc caatgattta cctgtatttc gtctatggta taaatctagt ttttccaatt
     5101 tttcagataa cttagcttta tcctctacag atataaaaga atttgttttc aaaatacctg
     5161 caaatttaca taaaatatga aaattctgta aatgtttatc tattacccag cgcgcagttg
     5221 tcatatctat tttcctattc tctctacaat ttttttaatt aacaatggag gaatagattc
     5281 accaatacat tgccgaatta ataattctga tgtatcgtcg ggaatattcc aatcaatagg
     5341 taatgatgtt aaaagcatta actctaatgg tgttaaaact cttgcatctg aatatgttcc
     5401 atcaggcata gaccgtccag gatgaacatt taattgtgaa ctgatagcgt cattacgaat
     5461 agtaatagtt ggggcaggag catcccactc catacggcga taagttgtat tataactttt
     5521 aattttttca ccattttttt tctttggata atgttctata ttatcaaaag cagatcgtcc
     5581 tgttggcgta ttttttagcc atataatgtg ctccggagca tgtgtacgtg caaaatgcca
     5641 tttcacatta gacttttgtc cagactcaat actaggcaaa aaactaatag catcacgaac
     5701 agaaatggtt tttgtaactt tttccggcat tccccaaata gttgaatgtt tattcatacg
     5761 aataatagct cgtttacgat gttgtgcaac accataatcg gcagaatcaa aaatatgagt
     5821 ttgaatataa tattcgcaac caaataaatc ttgcaaaatt acttctactg tttgtaatgt
     5881 ccccttataa ggtaacttaa tttttaaaaa aaatggaaca ttttcaatca aaacaaaagt
     5941 tgggcgtttt aataaaataa attcgattac tctgaatatt aaatgattac gtttatcatt
     6001 agccatctct tgaatgttac gatttttccc tgcaacactc atgccttgac aaggaggaga
     6061 agcaattaaa aaatctaatc gattcggcac gctctgtatt aaattttgaa acacttcctc
     6121 atgaagtata tcacctataa tcattttact ttcgggatat agagctttat ataaattagc
     6181 acgttctggc accaattcat ttgcagctat aatcttaata cccgcattat gcaagtaggt
     6241 ttctgcaatc cctgcactcg aaaacaaaga agcccctatc atcatagaac caaacccaaa
     6301 ttaactatat aatctctaca aagcaataaa ttatgcctct ttaaacaaaa ttaggaatgt
     6361 aatttcacaa caaaatatca aatgttttat atcttacatt tgaattttcc taagcagcct
     6421 gaaaaccaag agtaggctgc ttttccatat tcaggcagcc tacacaatca ttttacaaac
     6481 tgcttcaaaa aattcaaatc gttatcaaaa aacaagcgca aatcgttcac gttgtaacgc
     6541 agcatcgcga aacggtcgag accgataccg aaagcgaaac cggtgtattt ttcagaatcg
     6601 atattcacat ttttcaacac gttgggatgc accatgccgc agccgccgac ttccagccat
     6661 ttgccgtttt cgcccataat gtcgatttcg gcagacggtt cggtgaacgg gaagaaagac
     6721 gggcggaaac gtacttgcaa atcatcgcgt tcaaagaagc gacggataaa atccgtgaac
     6781 actgctttca aatcggcaaa agttacgccc tcttctaccc acaaaccttc cgcctgatgg
     6841 aacataggcg agtgcgtggc atcgctgtcc acacggtaaa cgcggccggg ggcgataatg
     6901 cggatgggcg gctctttttt atcgagcata tagcggattt gaatcgggga agtgtgcgta
     6961 cgcaaaacat caccgttttc aacgtaaaac gtatcctgca tcgcacgggc aggatggttt
     7021 gcagggatgt tcagggcttg gaaattgtga aaatcgtctt cgatttcagg cccgtccgcc
     7081 acttcgaaac ccattccgtg aaagagttcg accacacgtt gcaaggtcag ggttacggga
     7141 tgcaggctgc cgccttcctg agcgcgtccg ggcagggtaa tgtcgagggc ttcggcggca
     7201 agtcgggctt gcagcttggc ttcgttgagg gcatcgcgtt tggcattaaa agccgtctga
     7261 aaccggtttt tgcattcatt gatatgcgca cctatggttt tgcgctcttc aggcgacatt
     7321 tgccccaaag ttttcagaag tccggtcaac tcgccggttt tgccaagata acgggctttg
     7381 atttgttcta gagcgttgaa gtcttgcgca gcttctactg cggcaatgcc ttctgcaacg
     7441 atgcggttta cattttccat aatatcaagt ctgtcaatta atgatatgcc acctgaaaca
     7501 acgaggccgc ctgtatagcc tgacatatca gactcttcag acgaccttat tttcttcacg
     7561 gtcaatccat tggataaatt ttct
//
LOCUS       DAXHPG010000100         7578 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXHPG010000100 DAXHPG010000000
VERSION     DAXHPG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.100"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..265)
                     /locus_tag="ACFM6F_001492"
     CDS             complement(<1..265)
                     /locus_tag="ACFM6F_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6164888.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKSTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFAERQNHINGIGNFWNRAK"
     gene            complement(571..1830)
                     /gene="rho"
                     /locus_tag="ACFM6F_001493"
     CDS             complement(571..1830)
                     /gene="rho"
                     /locus_tag="ACFM6F_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC6164889.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTQQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(2050..4434)
                     /gene="ppsA"
                     /locus_tag="ACFM6F_001494"
     CDS             complement(2050..4434)
                     /gene="ppsA"
                     /locus_tag="ACFM6F_001494"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC6164890.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTAEAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4845..5666
                     /locus_tag="ACFM6F_001495"
     CDS             4845..5666
                     /locus_tag="ACFM6F_001495"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC6164891.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5906..6568)
                     /locus_tag="ACFM6F_001496"
     CDS             complement(5906..6568)
                     /locus_tag="ACFM6F_001496"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6164892.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTDSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6581..7408)
                     /locus_tag="ACFM6F_001497"
     CDS             complement(6581..7408)
                     /locus_tag="ACFM6F_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC6164893.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNMDGAETNTPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPVALFRSLITRYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT         1594 a         1825 c         2197 g         1962 t
ORIGIN      
        1 gttttgcccg gttccaaaag ttcccaattc cattgatatg gttttgtcgt tcagcaaaat
       61 aactttcatc tgcttctact tcgccgtcaa acatttccaa atgcggactg ttttgataaa
      121 taagtaatcg taaacgatga aaataatagg ctgcggtact tttattaacg cctactaact
      181 ctgctgctgt tcttgcagtt acacctgcga caaataactc aatgagttta ttttgtttat
      241 accggcttag acgacttttt ctcatagggg caactctaac ttaatttgaa tttccctagt
      301 tatctaggac agccccaaat ttatacaaaa atgagcgcgg ttcggcgcaa ccttgaatca
      361 agttcccgca tcggttttca ttgccggtac ggatgcgttc aagccggctt tgcaaaggcc
      421 gcgctttcgg caagcggaca cggacactgc cggcggttgc gccgttaacg gggggaggga
      481 ggagctgcgc cggccgtgtg aatgaaagtg ccgtctgaaa cccgattttc aggcttcaga
      541 cggcatttcg cattaatgcg ggtggcgcgt ttatttgccg cgcatcagtt caaagaaatc
      601 atcattgttt ttagaggctt tgattttccc gattaaaaat tcggctgcct cgatttcgtc
      661 catcgggtgc aggaacttgc gtaagagcca catacgttgc aactggtcgt tcggcacaag
      721 cagctcttcg cggcgcgtgc cggatttgtt gatgttgatg gcggggaaga ggcgtttttc
      781 cgccatacgg cggtcgaggt gcaattccat attgccggtg cctttgaatt cttcgtaaat
      841 cacatcgtcc atacggctgc cggtttcaac caatgctgtg gcaatgatgg tcagcgaacc
      901 gccttcttcc acgttgcgcg ccgcgccgaa gaaacgtttg ggacggtgca gtgcgttggc
      961 atcgacaccg ccggtcagga ttttgcccga ggtaggcacg acggtattgt aggcgcgggc
     1021 aaggcgggta atcgaatcca gcaggatgac cacgtctttt ttgtgttcca ccatacgctt
     1081 ggctttttca agtaccattt cggcaacttg gacgtggcgt tgcgccggct cgtcaaatgt
     1141 tgaagaaacc acttcacctc ggacggagcg gctcatttcg gtaacttctt cgggacgctc
     1201 gtcaatcaag aggacgatga gctcgacttc aggatagttt gcggtaacgg cgtgggcaat
     1261 attttgcaac atcacggttt taccgctttt gggcggggca accaagaggg cgcgctgacc
     1321 tttgccgatg ggggaaatca gatcgatggc gcgtccggtc aggttttctt cggactttaa
     1381 gtcgcgttcc agcttcaact gttgggtcgg aaacagcggg gtcaggtttt caaacaggat
     1441 tttatggcgg catacttccg ggtggtcgcc gttgatggta tcaagcctga ccagggcaaa
     1501 atagcgttcg ttgtcttttg ggacgcgcac gcttccttcg atggtgtcgc ccgtatgcag
     1561 gttgaagcgg cggatttggg tgggcgagac atagatgtcg tcggggccgg caagatagga
     1621 cgtgtccgcg ctgcggagga agccgaagcc gtcgggcagg atttcaagcg tgccggagca
     1681 ggtgaaaccc tcgccttttt tcatcatctg gcggacgatg gcaaatacga ggtcttgttt
     1741 gcggaatcgg ttggcgtttt cgatgccgtg ttcttccgcc aattctaaga gtttggaaat
     1801 gtgcagggtt tgtaattcgg agacgtgcat aataatgatg tattttgaag aggaaaaaga
     1861 caggcagatg ccgtctgaaa gaagaagctg accgttgccg gttgctcggg gaagggggaa
     1921 ttgtaggcag tcggcgcgtg ggtgtcaaat attatcgcgg acggggcatc ggcagaaaat
     1981 gccgtctgag cggagctgct tggaaaaaaa tacccccgcg cttttcaggc tcgggggtat
     2041 gggcattgat tatttgttca attcattcgc caaatacagc caagtttcga tgacggtatc
     2101 cgggttcagg gaaacgcttt cgataccttc ctcaaccagc catttggcga agtccggatg
     2161 gtcggacggg ccttgaccgc agatgccgac atatttgttc tgcttgcggc aggcggagat
     2221 ggcaaggtgc agcatcactt tgacggcagg gttgcgttcg tcaaacgatt cggataccaa
     2281 gccgctgtcg cggtcgagac cgagggtcag ttgggtcatg tcgttcgagc cgatggagaa
     2341 gccgtcgaag tattgcagga attgttccgc caataccgcg ttgctcggca gctcgcacat
     2401 cataatcagg cgcaggccgt ttttgccgcg ttccaagccg ttttctttca gggctttgac
     2461 aacggcttcg gcttcgccca aagtgcggac gaacggaatc atgatttcaa cgttggtcag
     2521 acccatttca tcgcggacgc gtttcaaggc tttgcattcc aaggcgaaac agtctttgaa
     2581 gttgtcggcg acataacgcg ccgcgccccg gaagcccaac atcgggtttt cttcatgcgg
     2641 ttcgtatacg ttgccgccga ccaggttggc gtattcgttg gatttgaagt cggacatacg
     2701 gacgatggtt ttacgcggat aaaccgatgc ggccaatgtc gccacgcctt cggcgatttt
     2761 atcgacgtag aagtcgacag gggacgcgta accggcgata cggcgggtaa tttccgcttt
     2821 taattcgtcg tcttgtttgt caaattccaa caaggctttc gggtggatac cgatttggcg
     2881 gttgatgata aattccatac gcgccaagcc gatgccttcg ctgggcaggt tggcgaagct
     2941 gaatgcgagt tcggggttgc cgacgttcat catgactttg acgggtgctt tgggcatatt
     3001 gtccaaggcg acatcggtga tctgtacgtc caacaggccg gcatagatga agccggtatc
     3061 gccttcggcg caggatacgg tcacttcctg accgttttcc aagagttcgg tcgcattgcc
     3121 gcagccgacg acggcaggaa tacccagttc gcgcgcgatg atggcggcgt ggcaggtacg
     3181 gccgccgcgg ttggtcacga tggcggaagc acgtttcatc accggttccc aatccgggtc
     3241 ggtcatatcg gtaacgagta cgtcgccggc ttcgacggaa tccatctcgg aagcatcttt
     3301 aatcaggcgt accttgccct gaccgacttt ctgaccgatg gcacggcctt cgcataatac
     3361 ggttttatcg ccgttgatgg cgaagcggcg caggttgcgg ctgccttctt cctgggattt
     3421 gacggtttcg gggcgggctt gcaggatgta gagtttgccg tccaagccgt cgcgtcccca
     3481 ctcgatgtcc atcgggcggc cgtagtgttt ttcgatggtc agtgcgtaat gcgccaactc
     3541 ggtgatttct tcgtcggtaa tggagaagcg gttgcggtct tcttcgggga catcgacgtt
     3601 ggttaccgat ttgccggctt cggctttgtc ggtaaaaatc attttgatgt gtttcgaacc
     3661 catggtttta cgcaggatgg cgggcttgcc cgctttgagc gtgggtttga acacataaaa
     3721 ttcgtccggg ttgaccgcac cttgtacgac gttttcgccc agaccgtaag aggaggtaac
     3781 aaagacgact tgatcgtagc cggattcggt gtcgagggtg aacatcacac ctgatgcgcc
     3841 gctgtcggaa cgcaccatgc gttgaacgcc ggcggaaagg gcgacgatat cgtgttcgaa
     3901 gcctttgtgg acacggtagg aaatggcgcg gtcgttgtac agggaggcga atacatggtg
     3961 catcgcttct ttaacgtttt ccaatccgtt gatgttcaag aaggtttcct gttggccggc
     4021 aaatgatgcg tcgggcaggt cttcggcggt tgcggaagag cgtacggcaa cggaaatgtc
     4081 cgcgccgccg gcatcggcaa ccattttgtt ccatgccgct tcgatttcgg catcgagctg
     4141 ttcggggaag ggtgtgtcca aaatccattg gcggatttct ttgccgacgc gtgccagttc
     4201 ggcaacgtct tcgacatcca attttgccag tgcggcggaa atgcgttcgc tcagaccgtt
     4261 gtgtgcgagg aatgcgcggt aggcttcggc tgtggtggca aagccgccgg ggacgcgaac
     4321 gcctttttcg gtcagctgac tgatcatttc gcccagcgag gcgtttttac cgcccacgcg
     4381 ttcaacatct gtcatacgca ggttttcaaa ccaaattacg tagttgtcgg ccatttgtgt
     4441 gtccaatcca aaatatgtta aaaaagaaac aaatccgcgt gcttatttta agcgattcgt
     4501 tcctctgctg tcatgtgttt tatccgtttt aaaatcatga tgccgtctga aaaattgcgg
     4561 tttcggcgtg tgtagcggtt tgaaacttgc agccggtata cttctttttt tgggtatttt
     4621 ctttgtaaaa caggtggttt gaataggtta atgttttttc tgtttgattt ttttgtttat
     4681 tttttaaaat tttctgccaa aaaatacttt atataaataa ttttatttca aaattatatt
     4741 gtgtctgttt gggtgtaatc cgaggtaggc gtgctgcggg tgctttcctt gtgtctgccg
     4801 ctgctgttat gatgatattt ttattctgta tttaaggatg gaagatgagc agtccgcgcc
     4861 atgtgtttta catttccgac cgtaccggtc tgactgccga gaatatcggc gaggctttgc
     4921 tgaaccagtt tggcaatctg tcgttcaaac gccatacgca tccgtttgtc gatacgccgg
     4981 aaaaggcgcg cgcggtggtg gagaaggtca atcggagccg gcaggaaaac ggtcagcgtc
     5041 cgattgcgtt tgtcagtgtg gttgatgacg aaatccgtcg gattatcaaa ggggcggatg
     5101 cttttcagat taatttcttt gagacttttt tgggactgtt ggagaaagaa ctcaataccg
     5161 aagccacggc atccgagcag gggcatcaca gtatcggtaa tacgaagcgt tacgatgcgc
     5221 gtatggaggc ggtaaatttc tcattgaacc acgatgacgg ggtcagcgat aagaaccttc
     5281 aggaagcgga tgtaatcttg atgggtgtat cgcgttcggg caaaacgccg acctgccttt
     5341 acctcgccct gcaatacggc atccgtgcgg caaactatcc gctgattcct gacgatttgg
     5401 aatcggccga tctgccgcgt atggtcaagc cttataggga taagctgttc gggttgacca
     5461 tccagccgga acgtttgcag gccatccgcc aagagcgccg cccgaattca acttatgcca
     5521 aaatcgatac atgccgcagc gaggtggcag acgcgcagag tatgttcaga cggcatggga
     5581 ttccgtttgc gaatacgacg gataaatcag tggaagagct tgccgtacac atccttcagg
     5641 cgtgcaagct caaacgcagg ttttgacggg ttttgattcg gtttgaaggt ggaaatgccg
     5701 tctgaaatca ggtttcagac ggcagtttta tagtggatta acaaaaatca tgacaaggcg
     5761 acgaagccgc agacagtaca aatagtacgg agccgattca cttggtgctt cagcacctta
     5821 gagaatcgtt ctctttgagc taaggcgagg caacgctgta ctggtttaga tttaatccac
     5881 tatatgtttg tggggcggat atttttcagg gctgtatttt gtccagacat tcgagcagat
     5941 cgagcggcgt tcggatgtgg aaatccgcct gccatgagtc ggtatcgtct tcatctgaga
     6001 tgtagcccca ttcggcgagg acggttttca tgccggcgtt gcggcctgct tggatgtcgc
     6061 gttcggcatc accgacgtag agtgtgtgtt gcgggtcggc gtggatttgt ccgcacgcat
     6121 acagcatggg tttgatgctg ggcttgggtt cgccgcaggt gtcgccgctg acgacgacgg
     6181 cgggtgggat gatgaagccg agtttgggga cgagtttgtc ggtgaagcgc atgggtttgt
     6241 tggtgatgat gccccatttg atgccgcgtt ggttgagttc ggcaatgagt tcgttcacgc
     6301 cgtcaaagag ctcggtgtct tgggcgtagc ggcggtcgta ttcctccaga tattcggtgc
     6361 gccatcgggc atagtcgggg tggtcggggg tgatgcctgc gccgagtttg atcagtcctg
     6421 ccgcgccgtg gctggcttgg gtgcggatct cgtccatgct ttttgcaggt agtccgtggc
     6481 gagcgagcag ggtgttgagt gcgcctccga ggtcgagggc ggtgtcggca agtgtgccgt
     6541 cgaggtcgaa gagtacggcg tgtatcatgt gtgttccttt tcagacggta tttttgttct
     6601 gtttcaaacc ctgctcgaaa tcttccagca tatccaattc aaagcggctg aagcccgctt
     6661 tttcgcgcgc ttcgatgttc acatagccgc ggaagataaa catatcgtaa cgggtaatca
     6721 gactgcggaa cagggcgaca ggttccaaac cgcgttcgcg gcagaggtgt tgataccaat
     6781 ggttgccgat ggcgacatgg cccacttcgt cgcggtaaat gatgtccaat acgccgcagg
     6841 tttccgaatc gccgcgctgc gccaccttcg cgcgtatccc cggcgtaacg tccagcccgc
     6901 gcgcttccaa cacgcgcggt accaacgcca tacgcaacag cgggtcgtag gcggttttgt
     6961 acgccatatc ccacaaatgg ttgtgcgcct cgaaactgcc gtaatcgaag ccgaaagcgc
     7021 gcaatctgtc gcgcatcagg cggaaatggt acacctcttc cttcgccact ttcacccagt
     7081 cgcggacaaa ctgaaacggc agcgtgcgga aacggtatgc cgcgtccaaa gctagattga
     7141 cggcgttgaa ttcgatatgc gcaatcgcgt gcagcatcgc cgcatagcct tctgccgtgt
     7201 tcattttgcg cggggtcagt tgcgacggcg cgaccaaaac aggcttgtcc ggccgtcccg
     7261 ctcgggggaa gtccgccggc ggcgtgtttg tttcagcacc gtccatgttc tgaacggcgg
     7321 caaacgcctc atccgtcagc cgtccttttt catctgggtc gcccgaaagc agggcgcgtt
     7381 ccagcaaagc ataaatatcg agtttcatct caagtccgcc gtgttcggaa aacaaatatt
     7441 atagcgtttt aaaaaaaaca agatgaggca tataatctcc gcgattcggc attccgcgcc
     7501 caaaccgtca aatatagtgg attaacaaaa accggtacgg cgttgcctcg ccttagctca
     7561 aagagaacga ttctctaa
//
LOCUS       DAXHPG010000101         7552 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXHPG010000101 DAXHPG010000000
VERSION     DAXHPG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.101"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..419
                     /locus_tag="ACFM6F_001498"
     CDS             <1..419
                     /locus_tag="ACFM6F_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC6164894.1"
                     /translation="EAEWAGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKA
                     AAAGHGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHG
                     NLGWLLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="ACFM6F_001499"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="ACFM6F_001499"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC6164895.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFSERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1376..1723
                     /locus_tag="ACFM6F_001500"
     CDS             1376..1723
                     /locus_tag="ACFM6F_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC6164896.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="ACFM6F_001501"
     CDS             1728..2321
                     /locus_tag="ACFM6F_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC6164897.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFNHVF
                     SKQVRALGRAGDILVGISTSGNSANVIEAIKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="ACFM6F_001502"
     CDS             2385..2993
                     /locus_tag="ACFM6F_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC6164898.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3651
                     /locus_tag="ACFM6F_001503"
     CDS             3001..3651
                     /locus_tag="ACFM6F_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164899.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3691..4470
                     /gene="map"
                     /locus_tag="ACFM6F_001504"
     CDS             3691..4470
                     /gene="map"
                     /locus_tag="ACFM6F_001504"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC6164900.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4625..4924
                     /locus_tag="ACFM6F_001505"
     CDS             4625..4924
                     /locus_tag="ACFM6F_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6164901.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5162..5536
                     /locus_tag="ACFM6F_001506"
     CDS             5162..5536
                     /locus_tag="ACFM6F_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6164902.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5723..7189)
                     /locus_tag="ACFM6F_001507"
     CDS             complement(5723..7189)
                     /locus_tag="ACFM6F_001507"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC6164903.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1946 a         2287 c         1848 g         1471 t
ORIGIN      
        1 aagaggcgga atgggcgggc agcctgttcg gcctgcacaa atctttcggc ttcctcacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cagttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgctgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaggtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ctatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 attgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcaccgcgcg attggttgcc
      661 gtcggcagac aatgccgtct gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt
      721 tttcgtaatt tcgcgcgcca gcattaatcg gcgttcggag aacagttccg ccatatcggc
      781 aagcgtcgca ccgatgcggt gcggcgtttc aaacatcacg acgggaaacg ccgcccgcac
      841 ccatttggca aacagtttcc tgcgctctcc cgatttcggc ggtacaaaac cgttgaaata
      901 aaaatcggat cccgccacac cggccacact caaagccgcc atcaccgcgc ttgcgccgac
      961 aacagggaca actttaaacc cgacctcacg cacgcggcgg gcgagtttcg cgcccgggtc
     1021 gcacacggcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagata
     1081 gccgacaatc ttgtccgcca tctgccgttc gttgtgttcg cgcacgctga caagtttgcc
     1141 ctgaatgccg tacgcgctca aaagctgcgc ggtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcgcg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgtcg gaggctttct gcaagtgttt
     1321 ctgaaacata aaaaatgccg tctgaaaaac agacattata aaggttaaac cgattatgcg
     1381 cctaaaccac aaacagggcg aggcagggga agatgccgcg cttgccttcc tccaatccca
     1441 aggctgcaca ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgccgca tacagcattt ccccatccaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa cagaacaggt tgacaaacgt accgtgccgc ctcgacgcgg tactcatcga
     1681 aggcagccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccgcccat tttgccgaaa gcatccgtgc caagcaggaa gccggaaaag
     1801 tattggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaacgacg
     1861 gcaagattct ggcgtgcggc aacggcggtt cggctgccga cgcgcagcac ttcgccgccg
     1921 aaatgaccgg acgctttgaa aaagagcgca tggaactcgc cgccgtcgcc ctgaccacag
     1981 acacctccgc gctgaccgcc atcggtaacg actacggctt caaccacgtc ttcagcaaac
     2041 aggtacgcgc cctcggacgc gccggcgaca tcctcgtcgg catttccact tccggcaatt
     2101 ccgccaacgt catcgaagcc attaaagcgg cacacgaacg cgatatgcac gtcatcgccc
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatactcaa agacaccgac gttctgctca
     2221 acgtccccca tccgcgcacc gcccgcattc aggaaaacca catcctgctg atacacgcca
     2281 tatgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagcc cccgatgaaa cccaaaccgc
     2401 acaccgtccg caccctgatt gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg aagcgccgcc gtcggcgcga aatccgccgt cgaccgccga accaccggcg
     2521 cgcaaaccga cgacaacgtt atggcgttgc gtatcgaaac caccgcccgc tcctatctgc
     2581 gccaaaacaa ccaaaccaaa ggctacacgc cccaaatctc cgttgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgagaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtat acaactacat taccgtcgcc tccctgccgc
     2761 gcactgccgg cgacatcgcc ggcgacactt ggaacacatc caaagtccgc gccacgctgt
     2821 tgggcatcag ccccgccaca caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc ccaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgtcgtattg
     3121 ctcatctgta tcaccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcggca aaaccctcca aaccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcaacctg ccgacacagc tcaaaccgac
     3421 aggcagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cccacaaacg tcccccgtcc cgaaccccga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacac ccaaagaaaa agaaacgccc aaagaaaacc ataccaaacc ggacaccccg
     3601 aaaaacacgc cgcccaaacc ccataaagaa attctcgaca acctcttctg acccggcacg
     3661 gcaggcacac ccgcaatcca aggaagcatt atgaacggca tcatcatcaa aacccccgaa
     3721 gaaatcgaaa aaatgcgcga gctgggcaaa ctcgtcgccg aagccctcga ctacatcgga
     3781 caattcgtca aacccggcgt aaccaccgac gaaatcgaca aactcgttta cgactaccac
     3841 gtcaacgtcc aaggcggcta tcccgccccc ctgcactacg gcaacccgcc ctaccccaaa
     3901 tcctgctgca cctccgtcaa ccacgtcatc tgccacggca ttcccgacga caagccgctc
     3961 aaagaaggcg acattatcaa catcgacctc accatcaaaa aagacggctt ccacggcgac
     4021 tccagccgta tgtttaccgt cggcaaagtc tcccccatcg cccaacggct gatcgacgta
     4081 acccacgcct ccatgatggc gggcatagaa gccgtcaaac ccggcgcgac actgggcgac
     4141 gtaggctacg cctgccaaca ggttgccgaa aacgccggct attccgtcgt acaggaattc
     4201 tgcggacacg gcatcgggcg cggtttccac gaagccccgc aagtgttgca ctacggaaaa
     4261 aaagggcagg gccccgttct aaaaccgggt atgattttta ccgtcgaacc gatgatcaac
     4321 caaggcaaac gccacctgcg tatcctcaac gacggctgga cggtggttac caaagaccgc
     4381 tccctctccg cccaatggga acacgaagtc ttggtgaccg aaaccggcta cgaaatcctc
     4441 accgtcagcc ccgcctccgg caaaccctga agccggacgt atccgcccca taaaaacaaa
     4501 caatgccgtc tgaaagaaac ggcagatatg atatataata taaaaacagg cttgacccgg
     4561 cacattacga aaacaaagca aatcggaatt tgccccgcaa ccagacaaac ttaaaggaag
     4621 ttttatgaaa atatttgaaa atatagaaga tgttaaagcc atccgtaaaa agaccgggct
     4681 gaaccagata gacttctggg gcaaggtcgg cgttacccag tctggaggct cgcgctacga
     4741 aaccggccgc aaaatgccca aacccgtacg cgaactgctc cgcctcgtcc atatcgaatg
     4801 catcgatttg gcgaaagtca acaaaaaaga tatggaaatc gccgccctgt tgaaaaaaca
     4861 ccatcccgac ctgtatgccg aattgtccaa acagaccaag tccgaaagaa aaaaacaaag
     4921 ttaaaccgca acctccggat gcccgaaagt ttttcatttc cgaaaaacgc aaacaatgcc
     4981 gtctgaaaca ccggacaggt cgccgtatcc cgcctgccgc ccctgcctca aaccgccgaa
     5041 ccgcccgaac ccgccttttt acaaacttta tccaatttcc tgtttatttc aggatacgcc
     5101 gacattagaa tgtcaaacag ctcgaaacgg gcaaactcca catccatcca aaggaataaa
     5161 aatgaaactt ctgaccaccg caatcctgtc ttccgcaatc gcgctcagca gtatggctgc
     5221 tgctgccggc acgaacaacc ccaccgttgc caaaaaaacc gtcagctacg tctgccagca
     5281 aggtaaaaaa gtcaaagtaa cctacggctt caacaaacag ggcctgacca catacgcttc
     5341 cgccgtcatc aacggcaaac gcgtgcaaat gcctgtcaat ttggacaaat ccgacaatgt
     5401 ggaaacattc tacggcaaag aaggcggtta tgttttgggt accggcgtga tggatggtaa
     5461 atcctaccgc aaacagccca ttatgattac cgcacctgac aaccaaatcg tcttcaaaga
     5521 ctgttcccca cgttaatcag gcaacaaaaa acagcgtttt cagaaatgaa aacgctgttt
     5581 tttgactgtt ccattattca caaaagggaa aaaacgatta cctgccccgt gtaccaaaac
     5641 ctgccctgcc ggatgaaggg cataactggc agggacggcg tcaacaccat atgggggtac
     5701 ggcttttctt gaaagattcg gcttaaatat ccaatacttt cgcggtatag gcgataattt
     5761 catccgccct ttcagggttt tcgttcaact tgatgccgta acccggtacc agctctttca
     5821 gacggtcttc ccaagacggg gcgcgctcgg ggaagcattg gtgcatcagc cggatcatca
     5881 gcggcacagc ggtcgatgcg cccggcgacg cgcccagcaa tgcggcgagt gagccgtcgg
     5941 cgtgggcgac aatctccgta ccaaactgga gcacgccgcc tttttcggag tcttttttaa
     6001 tgatttggac gcgttgccct gcggtgatga gttcccagtc gtcggggttt gcctcggggt
     6061 agtattccag cagggaggcg aagcgttctt ctttggtttt acgcaattcg cccagcaggt
     6121 atttggtcag cggcatattc gcccagccgg cgcacagcat aggatagagg ttgtccatat
     6181 ggatggacag cggcaaatcc ataagcgagc cttgcttgag gaagttggaa cggaagcctg
     6241 cgtaagggcc gaacataagg tggcgtttgc cgtccacgtt gcgtgtgtcg aggtgcggga
     6301 cggacatcgg cggcgcgccg acggaagcct gcccgtacac tttggcgttg tgttgttcgg
     6361 cggtttcggg gttgctgttg cggaagaaca ggccggacac ggggaagccg ccgtagcctt
     6421 tgccttcggg gatgccggat ttttgcagca gggtcagcgc gccgccgccc gcgccgagga
     6481 agaggaagcg ggtacggagg gtgagctgcc cgtcggggtt gcgggtatcg gcggttttga
     6541 gcacccacgc gccgtcggat tcgcgtttga tgtcttcgac gtggcggttg aactcggttt
     6601 ttacgccctt gccctgcaaa tatttcacca tttggcgcgt cagccgtccg aaatcgacat
     6661 ccgtaccttc ggcggagtag ttggcggcga cgggttggtt ttcgtcccgg ccgcgcatca
     6721 tcagcggagc ccaatcggaa attttgttcc gatcggtgga aaattccata ttttcaaaaa
     6781 gtttttgggt tttaaacgcg tcataacgtt tttgaagata agaacaatgg tcttcattca
     6841 tcaccaaaga catatgcggc acggcattga tgaaggaatt gtcttccaac ttgccttccg
     6901 cgaccagcgt cgcccaaaac tggcggctga catgaaactg ttcggcaata ttgagggcgc
     6961 gcgccggatc gataatccca tttgcaccca acggcgcata gttcaattcg cacagcgcgg
     7021 aatgccccgt gccggcgttg ttccacgcgt ttgacgattc caacgccaca tcttccaagc
     7081 gttcaatcag ggtgatttcc caagacggtt cgagttcttt gagcaaaacg cccaaagtcg
     7141 cgctcataat gccgccgccc accaagacaa cgtctgtcgc ttcagccatg gtttactcct
     7201 aaaaaacagg catcttctgc ccttatggtt atttgccgta ctacaaacgc ctgaatcgca
     7261 aaagcaggga aaaccgacaa tggtgtgtgt ccgagtatgc tgtttcgggg ttggaatgcg
     7321 ttgcaagtat ggcttccgac accgcttcag gggcttgtaa tatgttatcg tgaatgtagt
     7381 ggattttact gggaaatgca aagtttttct gtcgcccgcc aagtcgggaa actgcgaaat
     7441 gaaaaataaa aatagttatt tatctatata tatcaaattt ttaatagata aaaaatcaaa
     7501 attgtttata tattaatttt taaaagattg tcagcatatt gcgttaagtt tt
//
LOCUS       DAXHPG010000102         7473 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXHPG010000102 DAXHPG010000000
VERSION     DAXHPG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.102"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            140..721
                     /locus_tag="ACFM6F_001508"
     CDS             140..721
                     /locus_tag="ACFM6F_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6164904.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(783..1637)
                     /gene="folD"
                     /locus_tag="ACFM6F_001509"
     CDS             complement(783..1637)
                     /gene="folD"
                     /locus_tag="ACFM6F_001509"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC6164905.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVKFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1860..1937
                     /locus_tag="ACFM6F_001510"
     tRNA            1860..1937
                     /locus_tag="ACFM6F_001510"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1895..1897,aa:Pro,seq:tgg)
     gene            1962..2038
                     /locus_tag="ACFM6F_001511"
     tRNA            1962..2038
                     /locus_tag="ACFM6F_001511"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1996..1998,aa:Arg,seq:tct)
     gene            2066..2141
                     /locus_tag="ACFM6F_001512"
     tRNA            2066..2141
                     /locus_tag="ACFM6F_001512"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2099..2101,aa:His,seq:gtg)
     gene            2277..2783
                     /gene="rfaE2"
                     /locus_tag="ACFM6F_001513"
     CDS             2277..2783
                     /gene="rfaE2"
                     /locus_tag="ACFM6F_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFC6164906.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2780..4558
                     /locus_tag="ACFM6F_001514"
     CDS             2780..4558
                     /locus_tag="ACFM6F_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC6164907.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRALGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4671..5495
                     /locus_tag="ACFM6F_001515"
     CDS             4671..5495
                     /locus_tag="ACFM6F_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC6164908.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLKEKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(6258..7046)
                     /locus_tag="ACFM6F_001516"
     CDS             complement(6258..7046)
                     /locus_tag="ACFM6F_001516"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC6164909.1"
                     /translation="MLTLYSETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     AGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPYTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(7260..7454)
                     /gene="thiS"
                     /locus_tag="ACFM6F_001517"
     CDS             complement(7260..7454)
                     /gene="thiS"
                     /locus_tag="ACFM6F_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC6164910.1"
                     /translation="MNIILNGGPAELHGTTVADLIAQTAPQKPFAVAVNTGFVAKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         1591 a         1922 c         2238 g         1722 t
ORIGIN      
        1 atgtatggcg taaaggaagc gttttaacgc cgtattggga aaatgtaaag ttctttatcg
       61 ggatttcttt aagtattggc gttttcggtg tcgtggcggt agaatcgggg tcagttaaga
      121 acaattatca ggaacggata tggaagaaaa aaacgactac accgacgcgg catccgacaa
      181 ccgcaacggg caggaaattg aagtcggaat tgccggcgca ggcgaccgga tacttgccgc
      241 gctgctgaac caattgttta cctttttggt tttgttggtg ccgtttgccg gactgattgc
      301 tttcgccatc aagaatgaag gcaggattgg cggcagtgag gaaatattcg ggctgctttt
      361 gggcatgact tccttttggg taggactggc gggcattttg gtttataccg tcgttcaaat
      421 ctactatatg agtcgggacg ggcagtcatt gggtaagaag atcatgagaa tcagggtgtt
      481 gaaaaccgac ggccgcaatc cgggttttgt cggcacggtt ttggtacgcg aaatcgcatg
      541 gtcggttttg gttgccatta ttgccgccgt tatcggtctt gcagtaggtg acaacggaga
      601 aaacgccatc aacctgctgg cattccttgc caactttgtc ctgctcttta tggtcaaacg
      661 cgaccgccgc acgctttacg acatactggc ggatacggtt gtcgtcaagc tgcccaaata
      721 aacggacacg gcaaaatgcc gtctgaaagc ctttcagacg gcatttttat cttcagaacc
      781 gctcaagcat cgtgcagtga agccgcgtgc agggtgtttt ccatcaatgt ggcaatcgtc
      841 atcggaccca cgccgccggg aacgggcgta atcatcgccg cccgttcttt tgccgtttca
      901 aatttcacgt cgccgcacag gctgccgtcg tccaaacggt tgatgcccac atcgataacg
      961 accgcgccgg gtttgatcca ttcgcctttg acaaagttcg gaatgcccac gccgaccacc
     1021 aaaatatcgg ctccggcaac ctcgtctgcc agattttcgg ttgcgctgtg gcagaccgtt
     1081 accgttgcgc gcgccagcag cagttccaaa gcctgcgggc ggccgacgat attcgacgcg
     1141 ccgaccacga ccgctttttt ccccttcgga tcaatgccgt aagcttccaa aagcgtcatc
     1201 acgcccttgg gcgtacacgg gcgcatcagc ggcattttga ccgccagcct gccgacattg
     1261 taaggatgga agccgtccac gtccttatcc ggcgaaatac gttccaaaac cgcctggctg
     1321 tcgaggtgct tcggcagggg cagctgaacc agaataccgt ccacttcgga atcggcattc
     1381 aggcggtcga ccagtgccag cagttcttcc tgcgatgttg attcgggcag ttcgtaagac
     1441 agtgatttga tgccgcattt ttggcaggca gttttcttgt tgcggacata aaccgcgctg
     1501 gcagggtcgc ctccgaccaa aaccacggcc aggcaaggca tgtgcagatt gtcctgttgg
     1561 cgttgcgcca ccgcttcggc aaccgcctgc aggcgttttt gcgaaacttc tttaccattg
     1621 atcagttgtg ccgacatatc cgtccttttg ttaaacaggt agaaaatgct gaaaattatc
     1681 gcattttccg tttgttttta cagcaggatg ccgaaaataa atttgccctg aaataaaata
     1741 cggctgtttg gacggattaa atattttttc aaatcaaaaa actatatttt tcatcttggt
     1801 gtcttgaaaa taaatattga cgcatctttc cctgagttgt atagttcgct tcttcaagtc
     1861 ggggcgtagc gcagcccggt tagcgcatct gctttgggag cagagggtcg tgagttcgaa
     1921 tcccaccgcc ccgaccaatc aatcagcacg aatcggcatg agcgcccgta gctcaaccgg
     1981 atagagcacc gaccttctaa gtcgggggtt acaggttcga ttcctgtcgg gcgtgccaag
     2041 aatttgattg aaaaccgttt ttatggtggc tgtagctcag ttggtagagc cccggattgt
     2101 gattccggtt gtcgtgggtt cgagccccat cagccacccc agataaaggc ccgtactttt
     2161 cggtacgggt cttttcccgt ttgcgcgtac tgtccgcgcc gccggctttg cctttcgcat
     2221 atgcttctgt atcatgtggg tttttctgtc ttttatggtt tttgtcggag gctttgatgg
     2281 ttgacgcttg gtctgtccct gattttgaat cgaaaatctg tccgcccgag gcgttggcgg
     2341 cgcgtttggc gttgttgccg cgcccgctgg tgtttaccaa cggctgtttc gacatcctcc
     2401 acagggggca cgttacttat ctggcgcagg cgcgttcgat ggggggcgcg ttggtgctgg
     2461 cgttgaatac cgatgcttcg gtgcggcgtt tgggcaaggg cggcgaccgc ccgattaatc
     2521 ctttggagaa ccgtgccgcc gttgccgccg ctttggaaag tgtggatttg gtaacgtggt
     2581 ttgacgagga tacgccggcg gcgttgattg aggcggtcaa acctgagatt ttggtcaagg
     2641 gcggcgattg ggctgcggat aagattgtcg gtgcggcaga aacgttggcg cgcggcggtc
     2701 aggtgttttc aattccgttt ctgcaccaga cttcgacaac gaagactttg gcaaaaatcc
     2761 gtgcggcaga gggcggaaaa tgacggtttt gaagccttcg cactggcggg tgttggcgga
     2821 gcttgccgac ggtttgccgc aacacgtctc gcaactggcg cgtatggcgg atatgaagcc
     2881 gcagcagctc aacggttttt ggcagcagat gccggcgcac atacgcgggc tgttgcgcca
     2941 acacgacggc tattggcggc tggtgcgccc attggcggtt ttcgatgccg aaggtttgcg
     3001 cgcgttgggg gaaaggtcgg gttttcagac ggcattgaag cacgagtgcg cgtccagcaa
     3061 cgacgagata ctggaattgg cgcggattgc gccggacaag gcgcacaaaa ccatatgtgt
     3121 gacccacctg caaagtaagg gcagggggcg gcaggggcgg aagtggtcgc accgtttggg
     3181 cgagtgtctg atgttcagtt ttggctgggt gtttgaccgg ccgcagtatg agttgggttc
     3241 gctgtcgcct gttgcggcag tggcgtgccg gcgcgccttg tcgcgtttag gtttggatgt
     3301 gcagattaag tggcccaatg atttggttgt cggacgcgac aaattgggcg gcattctgat
     3361 tgaaacggtc aggacgggcg gcaaaacggt tgccgtggtc ggtatcggca tcaattttgt
     3421 cctgcccaag gaagtagaaa atgccgcttc cgtgcaatcg ctgtttcaga cggcatcgcg
     3481 gcggggcaat gccgatgccg ccgtgctgct ggaaacgctg ttggtggaac tggacgcggt
     3541 gttgttgcaa tatgcgcggg acggatttgc gccttttgtg gcggaatatc aggctgccaa
     3601 ccgcgaccac ggcaaggcgg tattgctgtt gcgcgacggc gaaaccgtgt tcgaaggcac
     3661 ggttaaaggc gtggacggac aaggcgtttt gcacttggaa acggcagagg gcaaacagac
     3721 ggtcgtcagc ggcgaaatca gcctgcggtc cgacgacagg ccggtttccg tgccgaagcg
     3781 gcgggattcg gaacgttttc tgctgttgga cggcggcaac agccggctca agtgggcgtg
     3841 ggtggaaaac ggcacgttcg caaccgtcgg tagcgcgccg taccgcgatt tgtcgccttt
     3901 gggcgcggag tgggcggaaa aggcggatgg aaatgtccgc atcgtcggtt gcgccgtgtg
     3961 cggagaattc aaaaaggcac aagtgcagga acagctcgcc cgaaaaatcg agtggctgcc
     4021 gtcttccgca caggctttgg gcatacgcaa ccactaccgc caccccgaag aacacggttc
     4081 cgatcgctgg ttcaacgcct tgggcagccg ccgcttcagc cgcaacgcct gcgtcgtcgt
     4141 cagttgcggc acggcggtaa cggttgacgc gctcaccgat gacggacatt atctcggggg
     4201 aaccatcatg cccggtttcc acctgatgaa agaatcgctc gccgtccgaa ccgccaacct
     4261 caaccggcac gccggtaagc gttatccttt cccgaccaca acgggcaatg ccgtcgccag
     4321 cggcatgatg gatgcggttt gcggctcggt tatgatgatg cacgggcgtt tgaaagaaaa
     4381 aaccggggcg ggcaagcctg tcgatgtcat cattaccggc ggcggcgcgg caaaagttgc
     4441 cgaagccctg ccgcctgcat ttttggcgga aaataccgtg cgcgtggcgg acaacctcgt
     4501 cattcacggg ctgctgaacc tgattgccgc cgaaggcggg gaatcggaac atacttaaaa
     4561 aatacaccct ttttcaacac ggggcggtat ccgtccgaat ccgtccggat tctgccgccc
     4621 gacagactgc cgcccaggcg ggcggggttt caaacattga ggaaattcta atgaaatggc
     4681 tcttcaccct tttggcggta ctcaatattg ccgtattcgg cggcacggtg ggctataaat
     4741 tggccgttaa agcgacgggc ggcgtgccgg aaaaccgggc cgtcgaaaac acgccccccg
     4801 caacgcccgc cgccgacaat gcggcggcat ccgtggagga tacggcggca ctgctcaaac
     4861 cgggcgacat cctgaccgaa gaacaggcgg agcagttgcg cttgaagaaa gaagcggaac
     4921 agaaaaaact gaaagagaaa aaacagcgtg aagaaaaagc ccgccgcgaa aaactcgccg
     4981 ccgaaaaggc gcaggcggaa cgcgaaaacg gcgcggcgga tgccttatgc gccgcgcagg
     5041 caagcctcac gatggacgaa gatgactacc accgcatcaa agggcttttg ggcaaatggt
     5101 cgcacgttgc cagcaggagt gttgaaaaac gcaccgccaa agccaaacct gccgacaaaa
     5161 cctaccgcgt cgtcctgccc gtttccgccg atgccgaaaa tcaggcggcg gagctgtctg
     5221 ccaaaggttt caaccccata ccgtttgacg gcgcattgag tttgggtgtc ggcaacagcc
     5281 gggaaaacgc ccaagccctg caaaaccggc ttgccggcgc cggattcggc ggggcgcata
     5341 ttgtcgaaca ctttgccgaa gccgacaggc aggacgattc tttgagcgtg tcgcgtatga
     5401 cggttttgtt taccggcgtg aatgccgccg atgcggacga aattcgtaaa atcacgtccc
     5461 tatacggcaa actgaacctc aagtcttgca aataggcgcg aaagccggac gataaacgcc
     5521 aaacccaccg cgaaggtggg tttggcgttt cggttttgag tatctgatat ataaaaaggg
     5581 aatgtatgtc tcgtatatgc cgactccaag tgtgaaagtg atgatgggga aatatggtgt
     5641 ttaatggttc ggaacggacg acttccgagt ggttttgatt atacgctaat aataataatt
     5701 atcaataaaa atttaaaaat aaatgcagat tttttgtaat acgatgaaaa ataaacaaat
     5761 taaactattt gccgatgttg gtgattcggg tcggcaaact ttcattgtgt cggacaaatc
     5821 ggggtcggca gtcatagctg agggcttcgt gcctgacgga aataaggttg cccgtgtttg
     5881 aacatccgtc cgattgggcg cggacgaggt tgtcattcgg gggtgtttga gcgtggattc
     5941 ggggatgcaa aagcggtaaa accgttgcgg atgaaggctt acggacggct ttaggtattt
     6001 ttaagtgtat tcaaacaaca acaaaacgct tcttctattg ccgtatttag ggcttgcgcg
     6061 gcagcctgca caccgtggag gccgagtgtt tcgttggttg cggcatcggg acgggcggca
     6121 aggttttccg gccggttttt ccattttttc cgacaaagcc gtgatgccgg ggaatggcgg
     6181 tttgtccgtt tcggaccgcc gccgttttga gccgcgatgc cgtctgaagc cttcagacgg
     6241 catttttgcc gcctttttca atattccgcc gaatgccaaa acggctgtcc gactgtcggc
     6301 gtgctggctt gcgctttgtc gcgtgcttcg accggtccgg cttcaaatgc cagccgtccg
     6361 gattcgacgg cgagtgcgaa ggcgcgtgcc atattgactg gatcgccgct gcgggaaacg
     6421 gcggtgttca aaagcacgcc gtcaaagccc cattccatca cttgtgccgc ctgtgagggc
     6481 aaacccaagc ccgcgtcgat aatcagcggc gtgtagggca ggcgttcgcg caggacgttc
     6541 aacgcgtagg cgtgaaccgc gcccaaaccc gtgccgatcg gggccgccca cggcatcaac
     6601 gcctgacagc ccgcgtcgag caggcggcgg caggcaatca ggtcttcggt gcaataaggc
     6661 agcactttga agccgtcttt aatcaggatt tccgccgctt cgacaagctg gaacacatcc
     6721 ggctgcaagg tgtcgtcatc tccgatgagt tccaatttta tccaatcggt ttcaaacact
     6781 tcgcgcgcca tttgcgccgt cgttaccgct tcctgcacgc tttggcagcc tgccgtgttc
     6841 ggcaggacgg gaacgccggt ttcttgaagc agcgaccaaa acccctgacc gtgcgcctcg
     6901 ccgccgcatc ccgcgcggcg cagcgagacg gtaatcatcg cgggccgggc ggttcggacg
     6961 gattgtttga ggatttcagg ggtcgggtag gcggctgtgc cgagcagcag ccgcgaaggg
     7021 aaagtttcgc tgtacagggt gagcataatg ggttcctttg tatagtggat taacaaaaac
     7081 cagtacggcg ttgcctcgtc ttagctcaaa gaaaacgatt ctctaaggtg ctgaagcact
     7141 aagtgaatcg gttccgtact atttgtactg tctgcggctt cgtcgccttg tcctgattta
     7201 aagttaatcc accataacgt tgttttgggg acaggggtcg tctgaaaagg caaaaccgcc
     7261 tagccgccga ccaccggccg cacgatatcg attttgtcgt tttcgtttaa aacggtttcc
     7321 gcatacgcgc ctttggcgac aaatccggta ttcaccgcta cggcaaaagg cttttgcggc
     7381 gcggtttggg cgatgaggtc ggcaacggtc gtgccgtgaa gttcggcggg tccgccgttt
     7441 aagatgatgt tcatggtttc tcgatgtttc tgt
//
LOCUS       DAXHPG010000103         7390 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXHPG010000103 DAXHPG010000000
VERSION     DAXHPG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.103"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(146..2749)
                     /gene="pepN"
                     /locus_tag="ACFM6F_001518"
     CDS             complement(146..2749)
                     /gene="pepN"
                     /locus_tag="ACFM6F_001518"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC6164911.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLFVKINGAAADYVLEGEMLTIAGVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRATMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAVAFDYQGKRATEAVLLLA
                     EAEQTFPLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGVELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLCADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2961..3755)
                     /locus_tag="ACFM6F_001519"
     CDS             complement(2961..3755)
                     /locus_tag="ACFM6F_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC6164912.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWSEFAQAWK"
     gene            complement(3872..4006)
                     /locus_tag="ACFM6F_001520"
     CDS             complement(3872..4006)
                     /locus_tag="ACFM6F_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164913.1"
                     /translation="MPSERIFRSILRLVGVSSETVKQKFAGVELSYAGKAAGESLYCL
                     "
     gene            complement(4506..5375)
                     /locus_tag="ACFM6F_001521"
     CDS             complement(4506..5375)
                     /locus_tag="ACFM6F_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC6164914.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEIN
                     LAKCFSEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDIPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPIREADNTVTLHFYPAWESFPGEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5426..5962)
                     /gene="ruvC"
                     /locus_tag="ACFM6F_001522"
     CDS             complement(5426..5962)
                     /gene="ruvC"
                     /locus_tag="ACFM6F_001522"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC6164915.1"
                     /translation="MSATVRILGIDPGSRVTGFGVIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5965..6204)
                     /locus_tag="ACFM6F_001523"
     CDS             complement(5965..6204)
                     /locus_tag="ACFM6F_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC6164916.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6234..7244)
                     /gene="dusB"
                     /locus_tag="ACFM6F_001524"
     CDS             complement(6234..7244)
                     /gene="dusB"
                     /locus_tag="ACFM6F_001524"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC6164917.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTKKTLHRSDFTDEGGIVAVQIAGSDPQQMADAARYNVSLGAQVIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHKNLPAVARIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECTATILNHIRAMHAFYGDTAGV
                     RIARKHIGWYIDEMPDGEQARRDINCLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     TF"
BASE COUNT         1670 a         1947 c         1931 g         1842 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tataattaat atgactttgc ggccgttttg
       61 ccattgcgta ataaaacgat ggggaagtga tgataaaacg tgtgtgtaac tatatcagac
      121 ggcattgttt ttctgtttga cggcctcaat ccaaaatttt gccgacgatt tcgcccacgt
      181 ctttcgacaa tccttcctgc gcccgaatgc gctgcaatgc ttgtttcacc aagtttttgc
      241 ggtgcggctc gagcttgttg cagaggttga acgcctgcac caggcgggcg gcgacctgcg
      301 ggttaaagcg gtcgatttcg atgactttgt cggcgatgaa gcggtagccg ctgccgtctt
      361 ctgcgtggaa atgcgggacg ttgcggctga agctgccgat gagcgagcgg gctttgttgg
      421 gattttcgag gctgaacttc ggatgctgca aggcggtttg aacctgttgc agggtgtcgc
      481 tgcggcggct tgagccgacg agggcgaaat atttgtccat caccagcgcg tcgtctgaaa
      541 acttgtcggc aaactgagcc agcaggcggt tgcgcgtatc gctttcgttg ccgttaacgg
      601 cggacaggat gccccattcg tgggtcatgt tttgcgccat ttcgccgtat ttttcggcaa
      661 cggtttcgat gtgcgcgggg tcggcgcaca ggacgaaggc gcggcagacg ttgcgcagcg
      721 tgcgccagcc ggcagcttcg gggctgtact cgtagctttg gttttcctgc ttcgccgcct
      781 gacggttcaa ttcgtgccat ttcggcagga agcggacggc aagcgtatcc aacaaggctt
      841 cgcgcgcctg atggtagcgc agcgggtcga tgttttctgc gccgtcccac agttcggctt
      901 cagacggaac gcccaaaagc agggctttga aggcgttgtc caagaggtcg tctgaaatga
      961 ctttttcgac ggcggcaagc agtttttcat gtttcggcaa ctcgacgccg tctgaaagcg
     1021 cggcaaggtt ggcggcgacg gcgcggcggt agagcgtttg cgcggcttcc cagcgcgtga
     1081 aggcgtcgct gtcgtgggca agcaggagca gcaggtcatc gtcgctgtac ggatagttca
     1141 gatgcactgg cgcactgaac ccgcgcagca gcgagggaac gacggcttcg gttacgcctt
     1201 cgagcgggaa ggtctgttcg gcttcggcca gcagcaacac ggcttcggtc gcgcgtttgc
     1261 cctgataatc aaatgccacc gcttcgccgt tgcggttcag cagcccgatt ttgacgggaa
     1321 tcatcatcgg ctgcttgtcc gccatatcgg gcgtgggcgg cacggtttgt ttaatggtca
     1381 actcgaaaac attgtttttc agacgacctt ccgcttccaa aacgggcgtg cccgcctggc
     1441 tgtaccacaa ggcgaactgg tcgagattaa tgccgttcgc gtccgccatt gtcgcgcgga
     1501 aatcgtcgca ggtcacggct tgcccgtcgt ggcgttggaa atagagcttc atgccttttt
     1561 ggaagccctc ttcgccgagc agggtgtgat acatccgcac cacttccgcg cctttttcat
     1621 aaacggtcat ggtgtagaaa ttgttcatct cctcatagct ggcggggcgc accggatggg
     1681 cggtcgggcc tgcgtcttcg gggaactggt gctggcgcag caggcggatg ttctcgatgc
     1741 ggcgcacggc gcggctggcg cggtcgccgg aaaattcttg gtcgcggaac acggtcagcc
     1801 cttccttcag tgaaagctgg aaccagtcgc ggcaggtaac gcggttgccc gtccagttgt
     1861 ggaaatattc gtgtccgatc acggattcga tgccttcaaa atcggtatcg gtggcggttc
     1921 ggctgtcggc aaggacgaac ttggtgttaa agatgttcaa acccttgttt tccatcgcac
     1981 ccatattgaa atcgcctacg gcgacgacca tgaaaatatc caagtcgtat tccaagccga
     2041 agcgcgtttc gtcccacttc atcgcgtttt tcaaggattc cacggcaaag ccgaccttgg
     2101 gcttgtccgc ttcggtggtg taaaactcga ttttgacgtt tctgccgctc atggtggtga
     2161 aacggtcttc cgttaccgcc aaatcgcccg cgaccaaagc aaacaaatag ctcggtttgg
     2221 caaacgggtc ttcccatttc acccaatggc ggccgtctga acactcgccg ccgtcgattt
     2281 tgttgccgtt ggagagcaaa acgggatagc gttttttgtc cgcaacgatg gtggtcgtga
     2341 atttggacat cacatccgga cggtcgatgt agaacgtgat tttgcggaag ccctccggct
     2401 cgcattgggt aaacagattg ccaccggaag catacagccc catcagcgat ttgttttccg
     2461 ccggcaggat ctcggtttcc acttcgacgg tgaagcgttc ggacggcacg cctgcaatcg
     2521 tcagcatctc gccttccaac acataatccg ctgccgcccc attgattttg acgaacaaga
     2581 gtttcgccga accgtccaac accagcggct ctcctaccct ctgcggctcg accgtcaaac
     2641 gtgatttcac gacggtttgc ggttcggcaa tgtcaaaatg caggtcggtt tcaaggatgc
     2701 ggtaggcagg cgtttggtaa tctttcagat aacggacggt tttgctcatt tttttctttc
     2761 aatattgttt ggtttgactg gaaaaggctt cagacagcac ggatacagct tttaaagcgg
     2821 attaacaaaa atcaggacaa ggcgacgaag ccacagacag tacagatagt acgggctgta
     2881 ccggtttgaa tttaatccac catatgccgt ctgaaaccga aacggcggca caagcgtacc
     2941 gccttcggac agcaggtttt ctatttccac gcttgggcaa attctgacca atgcggttta
     3001 tcttctgttt gggcggaaag atagtttctg atccgcttga tttccatttc gtattcgtcc
     3061 gcgtcgaatc tgccgctgac cagacaaaaa cgcagataca tcaaatacgt attcacggca
     3121 tcggtttcgc aataattgcg gatttctttc agcctgcccg cgtggaacgc atcccacacc
     3181 ttgctgccgt ccatacccag cttgcccgga aaaccgcaca gtttcgccat atcgtccagc
     3241 ggcacgtttg ccctcggctg gtaaagcgcg agcaaatcca tcaaatcgca gtggcgttgg
     3301 tgataacggc tgatgtagtt gttccacttg aaatcgcggc tgtcgccgaa atcgccgtcg
     3361 cccatatccc aatagcgcgc ggcgttaatg ccgtatatca gggagcggta atgcagaacc
     3421 ggcaggtcga aaccgccgcc gttccaactg accagttgcg gcgtatgttt ttcaatcaac
     3481 tcgaaaaatt tggcaatcac gacttcttcg ccatcgtcca tctcgccgat ggtgccgaca
     3541 tggactttat cctgccccca acgcatgcag cacgaaatcg ccacaatctg atgaagatga
     3601 tgctgcataa aatccccacc cgtctgagca cggcgttttt gctgggcaaa cagcaccact
     3661 tcatcgtcgg gcagcgagga cggcagctcg tacaatgttc ggataccctg cacatcgggt
     3721 acggtttcaa tatcgaaagc caaaatcgta ttcatggcag taccttgcat tcaaaaacag
     3781 acttgcgcct attgtgtcat taaaaggcgg ataaaaaaag aggcaacccc ccacaggatt
     3841 gccccaatac ctcaaatcag agatttacgc ttcacaaaca atacaggctt tcgcctgcgg
     3901 ctttgcccgc gtagctcaac tctacgccgg caaacttttg tttcaccgtt tccgatgaaa
     3961 ccccgaccaa tcgcaagatt gaccggaaaa tcctttcaga cggcatttcc tgcctgtcgt
     4021 gtaattccat gtagcgaaat gtacgccatt ttctacgctt tgccaagcat tttttacaat
     4081 ataaatgtca aaacattaat tttataaaat tgctgaaaat attaaatata tgaattttta
     4141 ttttaaatat tttaataaat ataaatttaa ttttgattta tatttaaatt taagcataaa
     4201 atgttaaata ttaaagtcaa tatgaaaggc atatattaaa tatttattta taacgctatg
     4261 tttttaaaga aaattaattt taatatatta actagattgt ctgcagatat tcatgggttt
     4321 gcggtatttc ttccaaaacc tgcttcgaat tttccgacca agtcttaaaa atattgtttt
     4381 ttgagatact taaatagcag cgattatcaa atgaaatctg ttcatataat ctgccatttc
     4441 gcatttaaaa aaacaaacag gagtttcgac tcgaaacgcc tgatatgttt tgtaatttta
     4501 cgtagtcagt aaaaatcggg gctgccttcc ggacgggttt tgaaacgctt gtgcagccaa
     4561 aaatactgct cgggatgttc gcgcacgcgt tcctctataa aacggttcat gcgctgagcg
     4621 tcagcctgcg catcttcacc cggaaaggat tcccaagcag ggtagaaatg caatgtaacc
     4681 gtattgtccg cctccctgat ggggatggcg ggtatcactt ttgcatttgc aagcgcggca
     4741 atgcggctca agccggtaat cgttgccgtc tgaataccga aaaaatccac aaaaaccgaa
     4801 tcgttgcgtc cgaaatcctg atcgggcaga tacagaaacg gcgcgctgct tttgcggaac
     4861 tgtttgacga gggcgcgcag cccttcggtg cgcccgataa ggaagacgtt gtgatagcgg
     4921 ttgcggcctt tcaaaatctg ttcgtccaat atcttgtttt tttgatggga atacatactg
     4981 atcagcggga tatcctgatt aagcgcgtac accgccatct cgaacgcggt gaagtgcgga
     5041 tacaggatga tgactttttc ccccgccgcc agcgcgtcgt ccaaataatg cttattgcgg
     5101 tagcgcacca gcgatttcaa acgtccggca ggcgcgtacc aatataaacc gtattccaac
     5161 atcagtttcg ccatgtgttt gaaatgctgt ttcaacacgg ttttacgctt ttcctcactc
     5221 cattcggaaa aacattttgc caaattgatt tcgccgatac ggcggcgcgg tttgaccaga
     5281 aggtaggcaa gcaaacccgt caggtcggca atcttgtgca gcagcgcaaa cggcagaaac
     5341 tgcaaaacat acagtacaaa aaatataaat ttcatctcga tacacatttt cttttcagac
     5401 ggcaaaatac aaatgccgtc tgaaactatt gaaacctgcc gcgcttgacc tgcatccccg
     5461 aaggattgag tttggcggca agcccgtggt tgcgtaaggc gtgggtcagc gcgacggcaa
     5521 gaccgtccgc cgcatccgcc tgcggcgttc ccgaaagccc cagcatctgc accaccatat
     5581 gctgcacctg ttctttcgcc gccttgcctt tgccgactac cgcctgtttg acctgcaagg
     5641 ccgtgtattc cgaaacgggc agcttatggc tgaccaatgc cgccaatgcc gcgcccctag
     5701 cctgacccag catcagcgtc gatgcaggat tgacgttgac gaacacctgt tccaccgccg
     5761 cctgctgcgg cttgtaaacg gcaacgactt cgccgatgtg ccggacgatt acggcaatcc
     5821 tgtctgccag aggcgcatcg gcaggcgttt tgatgcagcc tgaggcaacg taaaaatgat
     5881 cgcgccccct gacatcgatg acaccgaaac ccgttacgcg gctgcccggg tcgatgccca
     5941 agatacggac ggttgccgac atattcacag caaaccgtgt tgaatcagtt ttttacgcaa
     6001 ggtattgcgg ttcagtccca acatgacgga ggctttggac tgattaccgc cgcattgctc
     6061 catcacacac accagcagcg gcttttccac ctgatgcaat accatatcgt acacgccgca
     6121 aggttcggta ccgttcaggt ctttgaaata ttgttctaaa ttttgtctga tgcattgaga
     6181 aatatcggga agggtatggg gcatgattgc actttcaaag gataatcaag tgttcagaag
     6241 gtattcgggc ggtaggcgca cgcccaactg tcggtttttt cagcaagtct ttcaagataa
     6301 cctgcaagca tgtcgtattg cgccgccgca ctgtccaagc agttgatgtc gcggcgcgcc
     6361 tgttcaccgt caggcatttc gtcgatgtac cagcctatgt gtttgcgtgc gatgcgcaca
     6421 ccggcggtgt cgccgtaaaa cgcgtgcatg gcgcggatgt ggttcaaaat agtagcggtg
     6481 cattctgcca aactcaaagc aggcggtaaa acaccgtgtt cggcataatg tttcaaatcg
     6541 cggaagaacc acggtctccc ttgcgcgcct cgccctatca tgatgccgtc tgcggcggtt
     6601 tgtttgagga cagcttgggc tttttgcggc gaagtaatgt cgccgttgac ccaaactggg
     6661 atgttcagac ggcatttggt ttcggcaatc aggtcgtaag ccgcttcgcc tttgtacatt
     6721 tgcgtgcgag tgcgtccgtg gacggcaagg gcggcaatgc cgcaatcttc ggcgatgcgg
     6781 gcgacggcag gcaggttttt gtggtcgtcg tgccagccca aacgggtttt gagggtaacg
     6841 ggtacgcccg ccgccttgac taccgcttcc aaaatggcgg caaccagcgg ctcgttctgc
     6901 atcagcgcgc taccggcttg gacattgcag acttttttag cgggacagcc catgttgatg
     6961 tcgataacct gcgccccaag gctgacgttg taacgcgcgg catccgccat ctgctgcgga
     7021 tcactgccgg caatctgcac ggcaacgatg ccgccttcat cggtaaaatc actgcggtgc
     7081 agggtctttt tggtatttct aagcgtcggg tcgctggtca gcatttcgca caccgcccaa
     7141 cctgcgccaa aatctcggca aagtcggcgg aacggtttgt cggtaatgcc cgccatcggc
     7201 gcaagtgcga tggggttgtc gataaaatag ccgccgatgt gcataatggg cccgcgtttc
     7261 aaaaaagtac gccattgtac attttttaag caggatttcc aatctccgga cgcgcccgcg
     7321 attggggcag gcaccgtttt atggcataat ccgcacacag attccctgcc ccgccactca
     7381 caggagggca
//
LOCUS       DAXHPG010000104         7351 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXHPG010000104 DAXHPG010000000
VERSION     DAXHPG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.104"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            217..429
                     /locus_tag="ACFM6F_001525"
     CDS             217..429
                     /locus_tag="ACFM6F_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC6164918.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            577..2160
                     /locus_tag="ACFM6F_001526"
     CDS             577..2160
                     /locus_tag="ACFM6F_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HFC6164919.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDADLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLTF
                     DRMKINSQLLLQTILGRFSVSSDNAGKQFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2242..3339
                     /locus_tag="ACFM6F_001527"
     CDS             2242..3339
                     /locus_tag="ACFM6F_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC6164920.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3453..4568
                     /locus_tag="ACFM6F_001528"
     CDS             3453..4568
                     /locus_tag="ACFM6F_001528"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC6164921.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4693..5148
                     /locus_tag="ACFM6F_001529"
     CDS             4693..5148
                     /locus_tag="ACFM6F_001529"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC6164922.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKICQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5219..5695
                     /locus_tag="ACFM6F_001530"
     CDS             5219..5695
                     /locus_tag="ACFM6F_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC6164923.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5683..6609
                     /locus_tag="ACFM6F_001531"
     CDS             5683..6609
                     /locus_tag="ACFM6F_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6164924.1"
                     /translation="MSEVKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTTEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6674..7231
                     /locus_tag="ACFM6F_001532"
     CDS             6674..7231
                     /locus_tag="ACFM6F_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6164925.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGFTEDLVQKATDKAVANESGNGLKN
                     TAGTIAMARTADPDSATSQFFINLADNASLDYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
BASE COUNT         1926 a         1811 c         1913 g         1701 t
ORIGIN      
        1 tgttaatcca ctataaatgc gccgattgcc aaggcgatga tgctcaacca gggtttttgc
       61 gaagcgttca ttgttgtcgt cctgatggga attgccgcca ttatgcgcaa gcgtccgtct
      121 ttttccaaac cgccgcaccg tttccgcgtt tgcccaagtt cacgccccga cttacaataa
      181 cgcccgtcag attgcagttc caacgaaagg aaaatgatgg aaacccttat cctcgacatc
      241 ggcggcatga gctgcggcgg ctgcgtcaaa agcgttaccc ggatattgga aggcgtaaaa
      301 ggcgtggcaa gcgtcgaagt cagtcttgaa aacaaaagtg cgaccgtcgg atacgacccc
      361 gcgcaaaccg atgcaggggc gttgattgaa gccgttgaag acggcggcta tgatgccgcg
      421 ttgaaataaa gcggcaaaaa tgccgtctga agccttcgcg cctcccagat gccgtctgaa
      481 gtacgctccg cgccttcaga tggcatttga tttagttgaa cgggtaggat gaattggtta
      541 aacttgcggg caatagtgcc ttcagacaaa caaaatatgc cgaacaaaaa gaaaaaagcc
      601 gcctttgcgc gtgctgcaac ggagaacggt gtgccggacg tgtccaacat caaaaattca
      661 tatgatgatt tgatgtatga atccaaagca ttttcccaaa ccgccatcaa cgccttggag
      721 gctcgcgccc gcatgatggg tttgcagccc gcgcccgccg ccaatgccaa ggtcttggag
      781 ctgggctgtt cgatgggcgg caacatcatc acgcaggctc tttattaccc ggatgcggaa
      841 tttgtcggta tcgacttgtc cggcaggcag gttgcgcagg gcaacgccat cattgaaaaa
      901 atgggcttga aaaatgtgcg tctggaagaa aaagatattt tgaccgtcga tgagtcattc
      961 gggaagtttg actatatcat cgtccacggc atttggtcgt gggtgcctga cgcagttaaa
     1021 gacaaaattt tttcgatttg ccggaacaac ttgaccgaac acggcattgc ctatatttca
     1081 tacaatgttt accccggctg gaaacggcag gagcagttgc gcgaaattat gtactttgcc
     1141 ggcagggatg tgcttgaaga acccttggaa gcacggacgc ggaaaggctt ggacgcgctc
     1201 aaggcgctgg cggaaatttt agaaaacgac aagggcttgg acggcggcgg caaacttccg
     1261 gcgattcaaa aaatattgaa tcataatttt tattacatcg cacacgaata tatggaggcg
     1321 tttaacgacc cgatttacgt caacggcttc atcgaatggg caaaccgcca ccggctggcg
     1381 tatatcgggg atgccgattt gcacgcgtct tttgtttctt ggatggcgga gcatacgcgg
     1441 gagcggattc tggcattggc agggggcgat tatattgcca aagaatttta cagcgatatt
     1501 ttatccgacc gccaattccg ccgttcgctt ttatgccgtg aggaagtcgg ggatactgtc
     1561 aggcgtgacg agtcggttgc cgtcgaagtg atagaaagtt tgaatttccg cccggcaaga
     1621 ggggaaacaa tcaactttga tgaaaacgat accctgcttt cgggcatacg cgatgtgatg
     1681 aaaaccggag agccgtttaa aacggaagat gttgcggaaa atctcgcccg ccgatttccc
     1741 ggtttgacat tcgaccgtat gaaaatcaat tcccagcttt tattgcaaac cattctcggg
     1801 cgtttttctg tttcatcaga caatgcgggc aaacaatttt ttgaagacca taaaacctat
     1861 gtgccggcgc gttttacaga ttatgccgcc gcctttgtgg aacacggcgc ggaagcgttt
     1921 gtccggcctg ccaaccgtta caacgaaagc accccctcat tcggatatgg gcatttgtac
     1981 attatgcggc aattgtcgcg gccgacgagc aaacaggcat tgattgaaac ggttgccgaa
     2041 aacttgaaca tcgtcagcac cacgcccgac ggtttgacat tccatccgcc tgccgaagtg
     2101 tatgtggaag aaatattggc agacttggca gacaggcatt ttctcgtttc ggcggattga
     2161 cgggcaagtc ccgacggcaa tccgtccgat atattttcaa acaggaaaaa cacacactac
     2221 gaaaagcggt acacgacaca tatgaaccag acatttacct tgcccgatac gcgcccgtat
     2281 ccccaaaatc cgattaagaa ccacctgctg ctcaatgcct accagttggc gcataattct
     2341 tcccaggctt cgcgcaaact ctcgtccggc caacttcaaa ccgaaatcag ggggatgctt
     2401 gagcaaaacc actatatcaa cctttccctc gcgttgacga tgtcgcccga tgccggaact
     2461 tatgccgcgc tgctttccag tgtgaacgcg gtactcgatt gcgagaaaga aggcgaagtg
     2521 cagtggttcg ccctgccggt cgtgctggtg tccggctgca agaaagaacg ggcaatcgag
     2581 atgaagctgc cgacggaggc attgtttgcc tgcctgcaaa actatccgca cctgcgcgcg
     2641 ttgacgcaag agacacaatg gctgccttat cttgtgcatt cgtccgattt gagtgcggtc
     2701 gcgccggatg agtggtggcg tgccaaacaa aataccgaag cggcggcgca acacttacgc
     2761 cgtttcgccc cgcgcccttt gctgttgccc gaagggcagt ccgtccacgt cgtttacgcg
     2821 ctgggtttcg gcagcggcaa ggttcagacg gcattgggtc agaaccttct tcaggcaggc
     2881 ctgcccttaa tgcaggtatg gcaggaaaat cttgcatcgg aaggtgttac gctgtttgcc
     2941 aatccacttt ccccggattc tccggtacgc gcgctttcag acggcagcca cacgcgccag
     3001 cgtatggcga tggatgtttt tgcggcaaac gcgatacgcg ccgtccgtat gcagagtccg
     3061 cgcgtcggcg tggtcgctgc ggcaaaggcg ggcggacagg ttttattcgg gtttaatgcg
     3121 accgacggcg cgtttgaagt cgtgccgcag gtgttttcgt ggcagctttc ctttaccgac
     3181 aacatcgccg tcatccagca aaatttcctc gacctgatgg cggagtgccg cgtggaacac
     3241 gtttacctgt tgcacaatcc cttgggcgaa caggaaagca tcccgagcta tgcggaagca
     3301 ttgaaacggg aagggcacaa tccgtttttc agcgcatagt gatttgtagt agtgataggc
     3361 tttctcattt atataataaa tcaaaataaa gagcacaaca ctttttcatt ctgtgttgtg
     3421 ccttgagtga aacgaaagga tgaattataa atatgaagat tgtaattgca ccggattcgt
     3481 ttaaggaaag cttgacagct caacaggtag ctgaagcaat aaaaagaggc ttccaacaat
     3541 cgatagcaga tgtggaatgt ctcctctgtc ctgtcggtga tgggggagaa ggcactgtag
     3601 atgctatccg acattctctt gacctagaag aaaaatgtct tcaagtgaca ggaccttttg
     3661 gacaaaaaga agtcatgcgc tattttcaaa aagaacaact agccctattc gaagttgctg
     3721 atttggttgg tcttggaaaa atcccgctag agaaacgaaa tccattacaa atccaaactc
     3781 gtggtattgg agaattgatt cgccacctca ttagtcaaga aattaaagaa atctatattg
     3841 gcgttggcgg tacggccagt aatgatggag gtattgggat tgctgctggt ttaggttatc
     3901 aattttatga tgaggatgga aataccttac ccgtttgcgg tcaatcctta ctaaacctag
     3961 cttctgtttc aacagaaaat cgctatgaaa ttcctgaaga tgttcacatt cgtattttag
     4021 cagatgttgt gagtccctta tgtggacacc aaggtgcgac ctatacgttt ggcaagcaaa
     4081 aagggttgga ttctactatg tttgaggccg tagatcaggc aatacaagat ttttatgaaa
     4141 aagtctcccc tgcaacatta aaacttaaag gagcaggagc tggtggaggc atcgctggtg
     4201 gtttgtgcgc ctttgctcag gcaagtatcg tatctggaat tgacacctgc ctggacttga
     4261 ttgactttga taagaaagtg tcagatgttg acttggttat cattggtgaa ggaagactag
     4321 atcgtcaaag tttagcaggg aaagcgccta ttggtgtagc aaaaagaacc cctgtcggag
     4381 ttcctgttgt cgctatttgt ggcagtctcg ttgaagattt gccttccctg ccatttgaaa
     4441 atatccaagc tgccttttct attttggaga aaagtgaacc tttagaagat agtttgaaaa
     4501 atgccagtct ctatttggag catacggctt ctaatattgg acacttatta aatatgccta
     4561 agatttagcc aaaccatttc ttccagatgg atgttttggc tggttctgcc ttatgcggtt
     4621 acgattaacc gaccgatgcc gtctgaaagg gtttccgctt cagacggcat cgcgccggaa
     4681 cagggagggg atatgaaaaa accgaaaatc ctttttgtct gcctcggcaa catctgccgt
     4741 tcgccgatgg cggaatacat tttgcgccgc cgcgccgccg aagcgggcat tccccttgaa
     4801 gcggacagcg cggggacatc gggctggcac gacggcgaag atatgcaccg cgagacggca
     4861 aagatattga aaaaacacgg tatcgatgct tcaggcttta ccagccgcaa aatctgccaa
     4921 agcgatgcgg cggcgtttga ctgcatcatc gcgatggacg gcaagaattt gtccgaattg
     4981 gaaagaacct tcggcaggcg gccggaaaaa atattcaagc tgaccgacct gatacccgaa
     5041 agcggttacg accatgtccc cgatccgtgg tatacgggcg attttgaaga aaccttcagg
     5101 cttgcggatg cgggctgtcg ggcattgttg gaaaagattt ccaaataaag caattgaata
     5161 caaatataaa accgcctcct gccgtatcgg ttgttcagac ggcataaaca gagaatttat
     5221 gaaaacaaat ttcaaacaga aaattatcga acaggcacgc agcgaaggct tgcaggtaac
     5281 cgctttgcgc gagcaggtat tagatattgt cttgcagcaa agcggcgtga ttaaagccta
     5341 caacgtcttg tcgcagatgc agcagcaaag cgagggcgtg cttgcgccgc cgaccgccta
     5401 ccgcgccctt gatttttggg cggagcaggg cgttttgcac aaagtggcgg cggtcaacgg
     5461 ctatattttg tgcagccacg cgcagcacga gtgcgacgac cattgccacg accacgaaga
     5521 agccgaagcg caccacagcg cgtttatttt ggtctgcacc gaatgcggca cggcggacga
     5581 gcaaaccctc agccacgagt gggcggcact gcgcgcaggc gttgccgaaa gcggctttgc
     5641 gctgaaagaa gaacacgttg ttttaactgg gatttgtaaa aaatgtcaga agtgaaaaaa
     5701 accaaagtcc acctgatttc aggttttctg ggaacaggca aaaccactgc gctcaaaagc
     5761 ctgatggcgc aaaaagaccc gaacgaaaaa tgggtcatca tcgtcaacga gttcggcgaa
     5821 atcggcattg acggcgcggt attgagcgac aacggtatcc ctgtggcaga aatcgccggc
     5881 ggctgtttgt gttgcaccgc cggcccgcaa atgggcgtaa ccgtgcagaa aatgctgcgc
     5941 gacgccaagc ccgaccgcct gatgattgaa gcaagcggac tggctcacgc agccagcgtc
     6001 atcgacgaac tcaaagccaa accgctggac agccttttgg aaatcggcgc cgtctttacc
     6061 gtcgtcaatc cgcgccagtt catcaacccc gattacgcgc aacaggcgtt gtataaagac
     6121 caaatcggca tctgcgacgt attggtcgcc agcaaaaccg atttatgcac caccgaacag
     6181 cttgccgaat ttcacgacaa agcggcaaaa ctgttcccgc ccaaagccaa agtggtcgaa
     6241 gtccaaaacg cacaactcga catccggtgg ctcgatattc ccgtcatcga aaaatcacgc
     6301 taccgcctca aagccctgcc ggacaacacc atgggcttcc agtcgcaagg tttcacattc
     6361 cccgccggac gcgatttcga cggcgaaaag ctgaccaact ttttcaacga cctgcccaaa
     6421 atgaccgaag gactcgtccg cgccaaaggc gtgtttcaag tattgggaac gtgggtgtgg
     6481 ctcaactggg tagacggaca gtggggcgcg aaccaagtct catggcggcg cgattcgcgt
     6541 tttgaattga ttgccaaatc gtttgatgcg gatttaatcg aacaaaaact taaagatgca
     6601 ttggaatagc gttaacgcgc tttcagacgg catatgaaat gaaaatgccg tctgaaggtc
     6661 tgaggaggat gggatgaaac ccaaatttaa aaccgtttta accgcgctgc ttttggcggt
     6721 ttccctgccg tctatggcgg caacccatgt tttgatggaa accgatatgg gcaatatccg
     6781 tttggttttg gacgagtcca aagcccccaa aaccgttgct aatttcgtgc gctatgcccg
     6841 aaaaggcttt tacgacgaca ccgtttttca ccgcgttatc gacggtttcg ttatccaagg
     6901 cggcggattt accgaagact tggtacaaaa ggcaaccgat aaagccgttg ccaacgaatc
     6961 cggcaacggc ttgaaaaaca ccgccggcac catcgccatg gcgcggacgg cagaccccga
     7021 ttccgccacc agccaattct ttatcaatct cgcggacaac gcttcgctcg actacaaaaa
     7081 cggacaatac ggctataccg ttttcggcag ggtcgaaagc ggaatggaca cagtttccaa
     7141 aatcgcccgc gtcaaaaccg ccacgcgcgg cttttatcaa aatgtacccg tacagcccgt
     7201 caaaatccgt cgcgttgttg tcggacagta acacgcagac agacgttcag acggcgtcgc
     7261 ccgtttcaca aaaaacgccg tctgaagtga aaaaatattt taaaacagac agttgatatt
     7321 gacaaattca aaccgaggat tttaaaatgc t
//
LOCUS       DAXHPG010000105         7224 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXHPG010000105 DAXHPG010000000
VERSION     DAXHPG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.105"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(16..771)
                     /locus_tag="ACFM6F_001533"
     CDS             complement(16..771)
                     /locus_tag="ACFM6F_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC6164926.1"
                     /translation="MKFARLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPKWQKGKVSSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNADAQMFCSGASVGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(951..2126)
                     /gene="nirK"
                     /locus_tag="ACFM6F_001534"
     CDS             complement(951..2126)
                     /gene="nirK"
                     /locus_tag="ACFM6F_001534"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC6164927.1"
                     /translation="MKRQTLAALIASVFALAACGEQAAKPAETPAATASAEAPAASNS
                     QAAAETPSSELPVIDAIVTHAPEVPPPTDRDHPAKVRVKMETVEKTMKMDDGVEYHYW
                     TFDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTS
                     TFSFKALQAGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTK
                     GKKGAQGLQPFDMDKAIAEQPEYVVFNGHVGSIAGDNALKAKAGETIRMYVGNGGPNL
                     VSSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFR
                     AFNKGALGQLKVEGEENPEIMTQKLSDTAYAGNGAASAASAPAASAPAASASEKSVY"
     gene            2524..4779
                     /locus_tag="ACFM6F_001535"
     CDS             2524..4779
                     /locus_tag="ACFM6F_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC6164928.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELVAWLDLTAQE
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVVSETRVKAIESILPYYHGVY
                     SDDPKFQTTREHFAMKNNTLPSQEAREKLFNFFFWTSWSASTNRPDENFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNVQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            4958..5491
                     /locus_tag="ACFM6F_001536"
     CDS             4958..5491
                     /locus_tag="ACFM6F_001536"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFC6164929.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5554..6738)
                     /locus_tag="ACFM6F_001537"
     CDS             complement(5554..6738)
                     /locus_tag="ACFM6F_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC6164930.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDAQRVADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6778..7182)
                     /locus_tag="ACFM6F_001538"
     CDS             complement(6778..7182)
                     /locus_tag="ACFM6F_001538"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC6164931.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1691 a         1720 c         1955 g         1858 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgtgca acggaagccc aagttgtgca agacgtattt
       61 ggattgtagg ctggtgcgga tgccgtagcg gaggaatgcg gcatagttgg acgagtcgct
      121 cgacccgaca gatgcgccgc tgcaaaacat ttgcgcatcg gcattgccgg aagaaagcag
      181 gctgctgttg aaatcttccg tccattccca aatcagaccg tgcatatcgt aaacgcccca
      241 gtagttcggg cggcctttgc cgacatcgtg caggccttta cgaccgccat ccgcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cagcgcaata
      421 ggcgttggcg gcaaaccagg aaacattggt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcaggct ctgcctgttt
      541 ggaactgacc ttgccctttt gccatttggg gtgactgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagccgccgc
      721 ttgggtgccg gcgagagccg cacagagaaa gagtaaccgg gcaaacttca tattatgcct
      781 cctgacaggc ggttaacatt gatttttcag acggcatttt aaacgagaac cgcgaatgcc
      841 gtctgaagaa tcaagcttcc aaagtaaaaa ggttgatgat taaaaaacca ctgccgtgcg
      901 taatgaagta cggcggcagt ggtttgttcc gctaatgacg gatatccgat ttagtaaacg
      961 cttttttcgg atgcagaggc tgccggagcg gaagctgcgg gagcggaagc tgcggaagcc
     1021 gcaccgttgc cggcgtaagc ggtatcactc agtttttgag tcataatttc agggttttcc
     1081 tcaccctcta ctttcaattg acccaacgcg cctttgttga atgcgcggaa gatagagtgg
     1141 tcgaccaagg tgtagctgcc tggaatgtcg actttgaatt cgacaatggc ggaaccgccg
     1201 gcaggaacaa ttgtactttg tacgttttcg ttaatcagtt taccaccttc aacatatact
     1261 ttgtcgaaga tttcgccgat aacgtggaaa gaagatacca agttaggacc accgttacca
     1321 acgtacatac gaatagtttc accagcttta gctttcagag cgttatcgcc ggcgatagag
     1381 cctacgtgac cgttgaatac aacgtattca ggttgttcag caatcgcttt atccatatcg
     1441 aaaggttgca ggccttgagc acctttttta cctttagtat agaagtcacc ttgtacgatg
     1501 tagaactctt tgtccacttt aggcagacct tctttaggtt caaccaagat caaaccgtac
     1561 ataccgttgg cgatgtgcat acctacagga gcaacggcac agtggtagat gtacaaacca
     1621 gcttgcagcg ctttgaagct gaatgtggaa gtacggcccg gagcggtaaa ggttgcagcc
     1681 gcgccgccgc cctgaccggt agccgcgtgg aagtcgacgt tatggggaac ggtagaagaa
     1741 ggattgttgg agaattccac ttcaaccgta tcgccttcgc gtacgcggat catgcggccc
     1801 ggaacatcgc cgtcaaatgt ccagtaatgg tattccacgc cgtcgtccat tttcatggtt
     1861 ttttcaacgg tttccatttt aacgcgtact ttagccggat ggtcgcgatc agttggaggt
     1921 ggaacttccg gagcgtgagt tacgatagca tcgattacag gcagttctga agaaggagtt
     1981 tctgcagcag cttgtgagtt ggaagcggca ggagcttcag cagaagcggt agcagctgga
     2041 gtttcagcag gtttagcagc ttgctcgccg catgcggcca gtgcaaatac agaagcgata
     2101 agtgcggcta aggtttggcg tttcatgttt aaagtttcct tatagtaaga aaagttaaga
     2161 atcctgatag gatggtttga aattttgtat gattatgtag gcgcttgttt ggtcatattt
     2221 tgatgtaaat caaattattc atggaatatg aatcttttac tacctaaata ctcctttaga
     2281 agtataaaaa aatacatcga aaagtttaat tttgtgtgaa aatgttagcg aaaatttaac
     2341 acaaatacga taaaggaggg tttgtataag aaaatgggaa tcaatatcat ataagttaat
     2401 ggttttaaag gaatattttt gacaaaatat ttctttactt gttaaaccgt gatacaatct
     2461 ttaacattca tatttgatga attaataccc aaccactttt accaaaatta aggagtactt
     2521 aaaatgggac agtacaagaa gctttggtac ttgctgtttg ccgttctggc ggtatgcttt
     2581 accattcttg gctatatggg cagcgaggtt tacaaaaaag ccccgcctta ccccgaacag
     2641 gtcgtttccg catccggcaa ggtcttgatg acgaaagacg atattttggc aggtcagtct
     2701 gcatggcaga ctaccggcgg tatggaggtc ggttcgattc tgggccacgg cgcatatcag
     2761 gctccggact ggacggcgga ctggctgcat cgtgagttgg ttgcctggtt ggatctgact
     2821 gcgcaagaaa cttacggtaa aaaatttgac gaagtttctc ctgaagaaca agctgttctg
     2881 aaaacacgtt tggctgacga ataccgcaac caaagccgtg ttaaggaaga tggttctgtt
     2941 gttgtcagcg aaacccgcgt gaaagccatc gaaagcatcc tgccttacta ccacggtgtg
     3001 tacagcgacg atcctaagtt ccaaaccact cgcgaacact ttgcaatgaa aaacaataca
     3061 ttgccaagcc aagaagcgcg tgaaaaactg ttcaacttct tcttctggac ttcttggtct
     3121 gcttcgacca accgtcctga cgagaatttt acttacacca acaactggcc gcacgaacct
     3181 ttgatcaaca acgtaccgac cactgaaaac tatatgtggt cattcaccag cgttgttctt
     3241 ctgctgatgg gcatcggttt gctgatgtgg ggttattcct tcctgaccaa acacgaagaa
     3301 gtggaagtgc cgtctgaaga cccgatttcc aaaatccaac tgaccccttc gcaaaaagca
     3361 ttgggcaaat acgtcttcct gacggtcgcc ctgtttgtgg tacaagtatt gctgggcggc
     3421 ctgaccgcac actacaccgt cgaaggccag ggtttctacg gtatcgacga ggcgttgggc
     3481 tttgaaatgt ccgactggtt cccctacgcc ctgacccgta cttggcacat ccaatccgct
     3541 atcttctgga ttgcaaccgg ctttttgacg gcaggtttgt tcctcgcgcc gattgtcaac
     3601 ggcggcaaag atcccaagtt ccaacgtgcc ggtgtgaact tcctctatat cgccctcttc
     3661 atcgtggtcg gcggttctta cgcgggcaac ttcttcgcgt tgacgcacat ccttcccccc
     3721 gaatttaatt tctggttcgg acaccaaggt tacgaatacc tcgatttggg tcgtttctgg
     3781 caactcctgc tgatggtcgg actgctgttg tggctgttcc tgatgttgcg ctgcacggtt
     3841 tccgccttca aagaaaaagg cgtggacaaa aacctgctgg caatctttgt cgcttctatg
     3901 gtcggtgtgg gcgtgttcta cgcaccggga ctgttctatg gcgaaaaatc cccgattgcc
     3961 gtaatggaat actggcgttg gtgggtggtt cacctgtggg tggaaggctt ctttgaagta
     4021 tttgccactg ccgccttcgc atttgtcttc tacaatatgg gctttgtccg ccgcagtacc
     4081 gccactgcct ccactttggc cgctgccgcc atctttatgt tgggcggcgt tccgggtacg
     4141 ctgcaccacc tgtacttctc cggctctacc tccgcctcta tggcaatcgg cgcctgcttc
     4201 tccgctttag aagtcgtgcc gctcgtgttg ctgggtcgtg aagcatacga gcattggtct
     4261 taccaacacc tgtccgactg ggcgaaacgc ctgcgttggc cgctgatgtg cttcgttgca
     4321 gtcgccttct ggaatatgat cggtgccggc gtattcggct tcctgatcaa tccgccgatt
     4381 tccctgttct atatccaagg tttgaacact tctgccgttc acgcgcacgc cgccttgttc
     4441 ggtgtgtacg gtttcttggc attgggcttc gtattgctgg ttgcccgtta tttgaaaccg
     4501 aacgtgcagt ttgacgacaa actgatgact tggggcttct ggctgctgaa cggcggtttg
     4561 gtgggtatga ttgccatcag cctgttgccg gttggcgtga ttcaggcata cgcctccatc
     4621 acgcacggtc tgtggtatgc gcgtagcgaa gagttcctgc aaatggaaat cttggatact
     4681 ctgcgttggg ttcgtactgc tgctgacttg atcttcatcg gcggtgcaat ctgtgtcgcc
     4741 atccaagcca ctaaaatcgt attcggccgc gacaaataag ttttgcggta tgaaatgagc
     4801 aactgcaccc gttttcgggt gcagtttttt attgcggatt gggacgggaa tggaaaagga
     4861 tgtggatttt ttattttaat cggctataca attgctgctt tcatgccgtc tgaagcacgt
     4921 ttcagatggc atcggtttac atcagataag gataaaaatg ggtatttacg attttcaaat
     4981 gaaagatgca gaaggcaatg cggttgattt gtcaggctat cgcggcaagg ttctgctgat
     5041 tgtcaacacg gcaacgcgtt gcggtctgac cccgcaatac gaggctttgc agaagctgta
     5101 tgcacaatat accgcagaag gcttggagat tttggatttt ccgtgcaacc agttccgcga
     5161 acaggctccc gaaagcagcg gagaaattgc ccaagtgtgt atgatgaagt ttggcacgaa
     5221 gtttaagatt ttcgacaaaa tcgaagtcaa tggagcaaat accgcgccct tgtatgccta
     5281 cctgaaatcc gtcaaaccgc aagataaagg caatcatctg tttaaagatt tcgtgttgaa
     5341 gctcgcagct ttgggagaga agcgcgacga aggcgacatc aaatggaact ttaccaaatt
     5401 cctcgtcaac cgcgacggcg aagtggtcga gcgttttgcg cctagtgtta cgcccgaaga
     5461 aatcgaagcc gatattcggg cattgctgta atctgccgcc gtcttgggaa atgccgtctg
     5521 aaggcttcag acggcatttg tccgtttgcg ggtttattcc gaaatatcgg caacaccgct
     5581 ttcggggaag atgggcaaat ccggcggtgg gagcagggta tccaaagtca gggcggacgc
     5641 atcgagtgtg gggtagcctt caaacgcgac cgaataaccg ctgtttccgt tcatacggat
     5701 tcgggcgtgc gcgcccagag ggaaagtgat tttgtcggca atgtgcccga agggaaagcc
     5761 cgtcagcacg gggattttcg ccgtgcgcga aacatggttg gcaacggcgg aaaaatcata
     5821 agacggatca tagacatctc gaattttttc catacggaaa ttgccgaaca cgatggcgcg
     5881 ctgtttcttc aaaatacccg aaagatacag cgtattgagc atacgttcga tgcggtaggg
     5941 ctgttcgccg acatcttcga ggaacaaaat gccgccgtcg atgtcgggca tataaggcgt
     6001 gccggcgagc gaggcgagga cgcttaagtt gccgccccac aatatgcctt cggtttcgac
     6061 atcggcgcgt tggatataag gaacatcaac ggtcaggcgg ttttgggttg cacccttgat
     6121 aaaggcatcc atcgtaaacg caccgggggc gggtttgcca aaatcgctat aagccatcgg
     6181 gccggcaaaa ctcatcatat tgccttttgc caacaatgcc agctggacgg cgcatacgtc
     6241 gctgaatccg aaaaacagcg tgccgtgttc gcgcatcctt gcgccgagcg aagcaaaatc
     6301 gatatgcggc agaatccgcg ccgcaccgta accgccgcgc aagcccatca gcactttagg
     6361 cgtggcgacg cggccggaag cgacctcttg gaaatcggcg acacgttgtg cgtccgtacc
     6421 agcaaaccgt tggaagcggc ggctgcccgc ctgttggttg gttacggtaa aaccggcatt
     6481 gtaaaggcgg gttaaggctg tgttgacgcg gttggtgtct tcggcaaagc ctgacgaagc
     6541 gacaacgcgc agaaggttgc cgtccgaact ttgacggcgt ggcgtttgga gaggcacggt
     6601 tcgggctttc acaacagaat gggaagaggg aaggggcgga acggatgtgg cggatgtgcc
     6661 gcaagcctga agcagtcccg cgccggcggc ggcggtgcag gttttcagaa aacgacggcg
     6721 ggaggtcggt tcggtcataa tttccctttc atacggtttc agacggcatc ggcgggctta
     6781 aagcagcttt ttaagctgtt ccgtccaatc ttgcggcagg gtcataccgt gttttcggac
     6841 ggggtttgcc caaatcggcg cagggaagga cgcatcgttt tcaaagcggg cgataatatg
     6901 ccaatgcagg tgcggcacga cattgcccaa gctggcgagg ttaatcttgg caggttgaaa
     6961 cacttggcgc atagcggctt cgactttgta caccatttcc atcaattcgc cgcgttccgc
     7021 tgccgaaagg tcggtcattt cggcaatatg cttacgccaa atgacgcggc agaatgcagg
     7081 cgaaccgctg tcgttatgga cggcgatgac gcgcagcttg ggggtttgca gcaaaatatc
     7141 ttcatcttgt gccgtgcaga tggggcaggg ttgggcggtc atttttcctt attcctgaaa
     7201 gtgtttaaaa aacggtagga taat
//
LOCUS       DAXHPG010000106         7155 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXHPG010000106 DAXHPG010000000
VERSION     DAXHPG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.106"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(93..452)
                     /gene="rnpB"
                     /locus_tag="ACFM6F_001539"
     ncRNA           complement(93..452)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFM6F_001539"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(531..1634)
                     /gene="prfB"
                     /locus_tag="ACFM6F_001540"
     CDS             complement(531..1634)
                     /gene="prfB"
                     /locus_tag="ACFM6F_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC6164932.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1737..2630)
                     /locus_tag="ACFM6F_001541"
     CDS             complement(1737..2630)
                     /locus_tag="ACFM6F_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC6164933.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIVKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2972..3421
                     /locus_tag="ACFM6F_001542"
     CDS             2972..3421
                     /locus_tag="ACFM6F_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6164934.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3495..3803
                     /locus_tag="ACFM6F_001543"
     CDS             3495..3803
                     /locus_tag="ACFM6F_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164935.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3919..4761
                     /locus_tag="ACFM6F_001544"
     CDS             3919..4761
                     /locus_tag="ACFM6F_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC6164936.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNTVNHGLLSPEAFLENVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4751..5497
                     /locus_tag="ACFM6F_001545"
     CDS             4751..5497
                     /locus_tag="ACFM6F_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC6164937.1"
                     /translation="MQPETSAQYQHRFAQVIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5519..6106
                     /locus_tag="ACFM6F_001546"
     CDS             5519..6106
                     /locus_tag="ACFM6F_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC6164938.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6112..6291
                     /locus_tag="ACFM6F_001547"
     CDS             6112..6291
                     /locus_tag="ACFM6F_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC6164939.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6523..6963
                     /locus_tag="ACFM6F_001548"
     CDS             6523..6963
                     /locus_tag="ACFM6F_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC6164940.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1833 a         1897 c         1631 g         1794 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaaacacaa cctaaataaa aatgccgtct gaaccatatt
       61 tcaggtttca gacggcattt gcgtgtcgga tgcacaccgg acgggcggta agccgggttc
      121 tgtctcggac agtcattcct ctaggcatac cgttaccggt atgctcaagc aacctacccg
      181 aacgctcggc gggcagcgtc attgcgttct gtttggtctt gctccgaatg gggtttggcc
      241 tgccgcatat tgttaccaaa tgcgcggtgc gcccttaccg caccttttca cccttgcctg
      301 tgctgccaaa gcagccatcg gcggttttgc tttctgttcc actttccgtc gcgttaccgc
      361 gcccggccgt taaccggcat tctaccctgc ggagcccgga ctttcctccc cgtatgcctt
      421 acgcgatacg cggcgactgt ctgcccgtcc cgtgtgcggc gcggattata acacgaaacg
      481 caaaaatgcc gtctgaaacg gtacaggttt cagacggcat acggttcaaa ctacacgccc
      541 tgtttcaggc tggcttcgat aaagccgtcc aaatcgccat ccaatacggc tttggtgttg
      601 ccgacttcgt agcctgtacg caagtctttg atacgtgagg aatccaaaac atacgaacgg
      661 atttggctgc cccaacctac atcggattta ccttcttcca acgcctgttt ctcttcattg
      721 cgtttgcgca tttccaattc atacagtttg gacttcaaca tttccatcgc ggccgctttg
      781 ttggcgtgtt gcgaacggtc gttttgacat tgcaccacaa tccccgtcgg ctcgtgggta
      841 atacgcacgg cggagtcggt tttgttgatg tgctgaccgc ccgcgcccga tgcgcgatag
      901 gtgtcgatac gcaaatcggc ggggttgatt tcgatttcga tggaatcatc gatttcaggg
      961 taaacgaaca cggaggcaaa cgaagtatgg cgtttgttgt tcgagtcaaa cggtgaatag
     1021 cgcaccaagc ggtgaacgcc agtttcggta cgcagcaaac cataggcgta ttcgccttcc
     1081 actcggatag tcgcgcggtt gatgcccgcg atttcgccgt cgtcttcttc gaggatttcg
     1141 attttgaagc ctttgcgctc ggcgtagcgg ctgtacatgc ggaacagcat acccgcccag
     1201 tcttccgctt ccgtaccgcc cgcgcctgcg gtgatgtcga taaagcagtt gttcgggtcg
     1261 gcgggctggt tgaacatccg tttgaactcc aaatccgcca tctgtttttc cagccccgcc
     1321 acatcttcct gcacggcggc aaaaccttct tcgtcgtttt cttcgacggt catttcaatc
     1381 agcatgcggt tgtcttcgat gcccgaagcg atgttgtcga gcgtcaacac gatgccttcg
     1441 aggattttgc gctctttgcc gatttcttgg gcgcgtttcg ggtcgttcca aagttcgggg
     1501 tcttcggaaa gaccgataac ttcttccaat cggtctttct taccctgata atccatataa
     1561 actcggatgt cttcgctgcg cttttccaaa tcattcaggg tattgttgag ctggttgatt
     1621 acttcggctt ccatgattct tttgttcttt caaaatttta ggggcgtatt gtacgggatt
     1681 cgggtatttt tttctatgga taaagccttc tagaaacacg ttcggacggc atagcgtcaa
     1741 taacggtatg ccgccagttt gcgtttgatt tcaggcaatg cggcacgtgc tgcctcctca
     1801 cccaactgga tggcacgttt tttctgatcg aatccgccga ctgcacccaa atccaaaacc
     1861 tgcggtttga taaccacatc cgcctgcccc aactcatttt gcaacgcgga aacgctcatt
     1921 acgttcagcg tctgatcgag ataagagaag aagccttggc tgatgttttt gcccggacgg
     1981 gcggaaatat cgacggcaat cacgaaattc gccccctgcc gccgggcggc actgacgggc
     2041 acgggctgcg acagaccgcc gtcaacatat gtatgcctgc cgatgataac gggttggaac
     2101 acattgggaa tggcggcgga agcgcgcaca gcctgcccgg cattcccttg attgaaagcg
     2161 acggccttgc cggtttcaaa atcagtagca acagcggcaa atttgatggg aaactgctga
     2221 atctgcctgc cgccgacttt tcggttgatg taattttgca gcttttcgcc tttgataaaa
     2281 ccactggtgg acaaggttaa atcgactaaa tcggttttgc ctaaaatttc ggcttccaat
     2341 tcgaggcggt cgggcgacat acccgatgca aaaaggctgc cgacaatcga acctgccgat
     2401 gtgccggtaa ccaccttcac aggaataccg ttttctttca aaaccttaac aatacctaca
     2461 tgggcaaatc ctttagatgc gccgccaccg agtgccaaac cgaccactgc ggcgggtttg
     2521 gcggtttgca ccggcttgcg ggcagcatta tttcccgccg tgccgcaggc ggcaagcaac
     2581 gcggcggcgg cgattgccaa aagcggtctg atttttgaaa acgttaccat attttccatt
     2641 cctttatata tcgcaccccg tcaaaaagag ggattgcttt tcttaacaac cccctttgac
     2701 agccaagcaa atgggggctt tgttaagtca tcatcaaaat taatatttct ttttttcctt
     2761 tacggaaatt atatttgaag gcatactatc caaggcggga attatctcac aacaccgccg
     2821 ttatccaaat atcccgcctt tttcctttcc tttccatcaa aatactttct ttttatattc
     2881 attaacttgt taaatcattg gctgccgggt gtcagttttt ccgacaaaat ccgtctaatg
     2941 gggtatcaac agaaccaaaa caggaacact tatgaaaatc ggaacaactt ggcagacggc
     3001 atccgctatg ctggttttgc gtctgtttgc cgcatatgaa tttttggaat cgggtttgca
     3061 aaaatggaac ggggagaatt ggttttccga aatcaacgat cagtttccat tcccgttcaa
     3121 cttgctgccg gacgcgttaa actggaatct cgccatgtat gcggagcttt tactgcccgt
     3181 actgttgctt ttgggtttgg caacgcgtct gtcggcatta gggctgatgg tcgttaccgc
     3241 cgtcgcttgg gctgcggttc acgccggttc gggttacaat gtctgcgaca acggttataa
     3301 aatggcttta atttatatcg tggtattaat cccgctgctt ttccagggtg cgggcggatg
     3361 gtcgctggat acgctgctga aaaaacggtt ttgcccccaa tgccgtctga agcaagattg
     3421 attcagtcgt ggaatctgac tttaaacatt ccaaccttat ctcgttaact tgatattctg
     3481 aaaaggaaat agaaatgaac aaaaacattg ctgccgcact cgccggtgct ttatccctgt
     3541 ctttggccgc cggtgcagtt gctgccaaca aaccggcaag caacgcaaca ggcgttcata
     3601 aatccgccca tggctcttgc ggcgcgtcca aatctgccga aggttcgtgc ggcgcggctg
     3661 gttctaaagc aggcgaaggc aaatgcggcg agggcaaatg cggtgcgacc gtaaaaaaaa
     3721 cccacaaaca caccaaagca tctaaagcca aggccaaatc tgccgaaggc aaatgcggcg
     3781 aaggcaaatg cggttctaaa taatcccacc ccttcaaacc aagccgcgtt tttcagtaaa
     3841 atgcggcttt tttaacggca aacaaagatt ttttaacaag cacatcattc ttttgtgcca
     3901 tccgaaccgg gtaaaaatat gattcaacac gcaggcttgg gctaccgccg cgacttggcg
     3961 gaagactttc tctcgctttc cgaaaacagc ccgatatgct ttatcgaagc cgcaccggaa
     4021 aactggctga aaatgggcgg ctgggcgcgc aaacagtttg accgtgtggc ggaacggctg
     4081 ccgctggcgt tgcacggatt gtctatgtcg ctgggcgggc aagcaccgct ggatactgat
     4141 ttgatagacg gcatcaaaga aatgatgcgc cgttacgatt gcacgttttt ctccgaccat
     4201 ttgagctact gccacgacgg cggtcatctt tacgatttgt tgccgctgcc cttcaccgag
     4261 gaaatggtgc atcatacggc gcggcgtatc cgtgaagtgc aagaccgttt gggctgccgc
     4321 atcgccgtgg aaaacacatc ctactatctg cattccccgc ttgccgagat gaacgaggtc
     4381 gagttcctca acgccgtcgc acgtgaggcc gattgcggca ttcatctgga tgtgaacaat
     4441 atctacgtca acaccgtcaa tcacggcctg ttgtcgccgg aggctttttt ggaaaatgtg
     4501 gatgcagatc gcgtgtgcta tatccatatt gccggacatg acgtggaaac gccggaattg
     4561 ttgattgata cacatggcgc ggcagttttg ccgactgttt gggacttgct cgaacttgcc
     4621 tatgccaagc tgccgacgat tccgcccacc ctgttggaac gcgattttaa tttcccgcct
     4681 tttgccgaac tcgaagccga agtcgccaaa atcgccgatt atcaaacgcg tgccggaaag
     4741 gaataccgcc gtgcagcctg aaacctccgc ccaataccag caccgtttcg cccaagtcat
     4801 acgcgggggc gaagccgcag acggtctgcc gcaagaccga ctgaacgtct atatccgcct
     4861 gatacgcaac aatatccaca gctttatcga ccgctgctat accgaaacgc ggcaatactt
     4921 tgacagcgaa gaatggagcc gtctgaaaga aggtttcgtc cgcgacgcgc acgcccaaac
     4981 gccctatttt caagaaatcc ccggcgagtt cctacaatat tgccaaagcc tgccgctttc
     5041 agacggcatt ttggcactga tggattttga atatacccaa ttgctggcag aagttgctca
     5101 aattccggat attcccgaca ttcattattc aaatgacagc aaatacacac cttcccctgc
     5161 ggcctttatc cggcaatatc gatatgatgt taccgatgat ttgcatgaag cggaaacagc
     5221 cttgttaata tggcgaaacg ccgaagatga tgtgatgtac caaacattgg acggcttcga
     5281 tatgatgctg ctagaaataa tggggtcctc cgcgctttcg tttgacaccc tcgcccaaac
     5341 ccttgtcgaa tttatgcctg aggacgataa ttggaaaaat attttgcttg ggaaatggtc
     5401 aggctggact gaacaaagga ttatcatccc ctccttgtcc gccatatccg aaaatatgga
     5461 aggcaattcc ccgggccaaa accatctatc cgcataaaat taccttgttc ccgatactat
     5521 gccgctaccc gacctgaccg atgccgaatt aatagagtcg cgtaaactgc ttctgcattt
     5581 tgcgcggctt cagttgcccg accaccctga tttggctgaa gatttagtgc aggaaacatt
     5641 gctgtccgca tacagcgcag gcgacagttt tcaaggcagg gcacttgtca acagctggct
     5701 ttttgccata ttgaaaaaca aaattattga cgcattacgt caaatcggaa ggcagaggaa
     5761 agtctttacc acactggatg acgagctact ggatgaagca tttgaaagcc atttttccca
     5821 aaacgggcat tggacgccgg aagggcagcc gcaacattgg aacactccgg aaaaatcatt
     5881 aaacaacaac gaattccaaa aaattctgca aagctgccta tacaacctgc ctgaaaacac
     5941 cgcacgggta tttaccctga aggaaatact cggtttttca tccgacgaaa tacaacaaat
     6001 gtgcggtatc agcacgtcca actaccacac cattatgcac cgcgcccgag aatcattgcg
     6061 ccaatgcctg caaatcaaat ggttcaacca agaaaacccg aagtaaacgt tatgaaaaaa
     6121 tgccgcgata tcgccctgct tctttccaaa catcaggacc gggaaaccac cccgggcgag
     6181 aagatttcca tatacacaca cctgctgttc tgtccgtatt gccgtgaata taaaagacaa
     6241 cttcaaacca tcaaaagatc actggcaaaa acaaccagaa cttcaaaata aatgccgtct
     6301 gaaaaggctt cagacggcat aagctgacgg aaaaaaatca aaccgattta ctgttatcgg
     6361 cagttcatcc ataatacacg cttcaaaagc agcatattcc tccatacgga atatataaat
     6421 acgcaaaaca cgaaggctgc atcaatttgc tttatttgcc ttatttcaca gacggcgcta
     6481 cccctcccgc ccaacccgtt ctttctgaat gagcagattt caatgattaa ggaaacccta
     6541 atgcgcccaa tcttcctatc tttcgtttta ctccctattt tgataaccgc ctgcagcaca
     6601 ccggacaagt ctgcccgatg ggaaaatatc ggcacaatct caaacggcaa tattcataca
     6661 tatatcaata aagacagcgt gagaaaaaac ggaaatctga tgattttcca agataaaaaa
     6721 gttgttacca atctaaaaca agaacgtttt gccaacaccc ccgcatacaa gactgccatt
     6781 gccgagtggg aaatccactg caacaacaaa acataccgct taagttcgct acagttgttt
     6841 gatacaaaaa acacggaaat ttccacacaa aactacacag cctcttccct ccgcccgatg
     6901 agcatcctgt ccgggacatt aaccgaaaaa caatatgaaa ccgtatgcgg aaaaaaactc
     6961 tgattgcaac ttatacacaa acttacccac aaaccttatc ataaaaatgc cgtctgaaat
     7021 actgaaatat cagcatttca gacggcattt tgccatttcc ccgaaaagtt acccccaaag
     7081 ttatccacat tattttttaa accagcttcc atccgaaata tagtggatta acaaaaatca
     7141 agacaaggcg acgaa
//
LOCUS       DAXHPG010000107         7147 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXHPG010000107 DAXHPG010000000
VERSION     DAXHPG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.107"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            152..1090
                     /locus_tag="ACFM6F_001549"
     CDS             152..1090
                     /locus_tag="ACFM6F_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC6164941.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            1219..1512
                     /gene="hfq"
                     /locus_tag="ACFM6F_001550"
     CDS             1219..1512
                     /gene="hfq"
                     /locus_tag="ACFM6F_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC6164942.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPASTLVQVETVQQPAE"
     gene            1596..2162
                     /locus_tag="ACFM6F_001551"
     CDS             1596..2162
                     /locus_tag="ACFM6F_001551"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6164943.1"
                     /translation="MLLKNILPFAHCLLRQALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALNNTRCRLQEAGYSNVRFILDGHENLKQYIPKPLDAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAVGQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            2216..2590
                     /locus_tag="ACFM6F_001552"
     CDS             2216..2590
                     /locus_tag="ACFM6F_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC6164944.1"
                     /translation="MQYLFVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            2600..3094
                     /gene="folK"
                     /locus_tag="ACFM6F_001553"
     CDS             2600..3094
                     /gene="folK"
                     /locus_tag="ACFM6F_001553"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC6164945.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDTRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDR"
     gene            complement(3222..3701)
                     /locus_tag="ACFM6F_001554"
     CDS             complement(3222..3701)
                     /locus_tag="ACFM6F_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC6164946.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(3921..4658)
                     /gene="ubiE"
                     /locus_tag="ACFM6F_001555"
     CDS             complement(3921..4658)
                     /gene="ubiE"
                     /locus_tag="ACFM6F_001555"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC6164947.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(4701..5069)
                     /locus_tag="ACFM6F_001556"
     CDS             complement(4701..5069)
                     /locus_tag="ACFM6F_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC6164948.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQIADLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            5189..6808
                     /gene="mnmC"
                     /locus_tag="ACFM6F_001557"
     CDS             5189..6808
                     /gene="mnmC"
                     /locus_tag="ACFM6F_001557"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC6164949.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLNTPYAEPLCGLFWQHGVW
                     LNLPAFVRALLRHPLIGLHEDTPLTGISHDGEKWIASTPNGIFSATHIVYCTGAHSPC
                     LPEINLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKYQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(6922..7039)
                     /locus_tag="ACFM6F_001558"
                     /pseudo
     CDS             complement(6922..7039)
                     /locus_tag="ACFM6F_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
BASE COUNT         1901 a         2233 c         1450 g         1563 t
ORIGIN      
        1 ttccgattta aaaacagaat gttataaaac ccaatcccca aaacacaaca agaccgcccg
       61 cctacgggca gtctcctgtc agacgacata ctttacagat ggctgttttt tcaacaaaat
      121 aacgccaata ctcaaaaata tggaatcaaa aatgtccatc catactctga aacgcctgcc
      181 ctcatcgctg ctgctcggtc tctgcctttc cctgccgtca gcccaccttt ttgccgacaa
      241 cgacatttta gggcaatttt tagaacagaa catgcttacc tcctccgatc cgatagaaat
      301 attcgccgaa agcacgatac accccaccaa cacccaagcc attacaggcg gtctgattct
      361 ctcctcacag tctgccctgg tcgtcaacaa caaaaccgga cagatactgt atcagaaaaa
      421 cgccgacagg attatgccca tcgcctccat ttccaaactg atgagcgcga tggtcgtttt
      481 ggatgcaaac ttggacatga acgaaaccgt taccattacg cccgacgaaa tcgaccgcat
      541 caaagggacc ggcagccgtc ttgccatagg tacggcactt acacgcaaag aactgctgca
      601 cctgagcctg atgagcagcg aaaaccgcgc cacccatgca ttgggcagaa cctaccccgg
      661 cggcatgggc gcatttgtcg ccgccatgaa ccgcaaagcc caaagcctcg gtatgtacgg
      721 cagccgcttt tacgaaccga ccggactcaa cttccaaaac gtttctaccg ccaaagacct
      781 gagccttatg gtcaacgccg ccgcccaata tccgcaaatc cgcaccaact cgacttccaa
      841 ctacgcctcg gtacagacca aaaacgggca gcagaactac aaaaactcca atgccctggt
      901 cagagaaggc atgtggaaca tcgaattgca gaaaaccggc tacatacgcg aagcaggcag
      961 gtctatggtt gtcaaagcca acattcaaaa tcaacccgtt accatcgtat tgctgaactc
     1021 gcccacatcc gccacacgcg tcaacgacgc ccgcaaaatc gaatcgtgga tgctgcagca
     1081 acgctcctga catacaaatg cccggcggaa aaccgcccaa acacaaaaaa aattggtaat
     1141 ttttcctatt caaggctaca attcgacacg cacagggcgt ctgcccgatt tcacaatcat
     1201 aataacggag ttaaaaatat gacagctaaa ggacaaatgt tgcaagatcc cttcctgaac
     1261 gccctgcgta aagagcacgt tccggtttcg atttacctgg tcaacggcat caaactgcaa
     1321 ggtcaggttg aatctttcga ccaatacgtc gttcttctga gaaacacttc cgtaacccaa
     1381 atggtttaca aacacgccat ttccaccatc gtaccggctc gctccgtcaa cctccaacac
     1441 gaaaacagac cccaagccgc accggcttcg acccttgtcc aagtggaaac cgtccagcag
     1501 cctgccgaat aatccgcgcg aagcatgacg tgtcatatct ttcaatacct taccgacatc
     1561 ggtaaggtat ttttattttc agacagcatt taaaaatgtt attgaaaaac atccttccat
     1621 tcgcccattg ccttttgcgg caggcacttc ccgaaggcgg caatgcgctg gacggcaccg
     1681 ccggcaacgg acacgacacc cttttcctcg cacaaaccgc aggcatccgg gggaaagtgt
     1741 gggcattcga catccagccg caagccctga acaacacccg atgccgtctg caggaagcag
     1801 gttacagcaa tgtacggttc atcttggacg gacatgaaaa cctgaagcaa tatattccaa
     1861 agccgctgga tgcagccatt ttcaatttcg gctggctgcc cggcggggac aaaagcctta
     1921 ccacccgcac ggaaaccagc attgccgccc tttctgccac cttatcccta ctgaaagaaa
     1981 acggtatgct tattgccgtc ctctatccgg gacacgaaaa cggcaaacag gaggcagaag
     2041 ccgtcggaca atgggcaaaa aacctgcctc aagaacagtt tgccgtttta cgttacggct
     2101 ttaccaaccg gaaaaacagc ccaccctatc tgttggcatt tgaaaaactg cgtcaaaaat
     2161 aactgtttgc ggtaaaataa gccgattcca acccttattt tgataagagc cgaccatgca
     2221 gtatctgttt gtcaaataca gccaccaaat cttcgttacc atcaccattt tggtattcaa
     2281 catccgtttt ttcctacttt ggaaaaatcc agaaaagccc ttggcgggct tttggaaggc
     2341 actgccacac cttaacgaca ccatgctgct gtttacggga ttgtggctga tgaaaattac
     2401 ccatttctcc ccgttcaacg cgccttggct cggtacaaaa atcctgcttc tgctcgccta
     2461 tatcgcattg ggtatgatga tgatgcgcgc ccgtccgcgt tcgaccaagt tctacaccgt
     2521 ttacctgctc gccatgtgtt gcgtcgcctg catcgtttac cttgccaaaa ccaaagtcct
     2581 gcctttctga aacaccgtta tgaacaacag acattttgcc gtcatcgccc tgggcagtaa
     2641 tcttgaaaac cctgcccaac aggtacgcgc cgcattggac acgctgtcgt cccatcctga
     2701 catccgtctt aaacaggctt cctcactgta tatgaccgcg cccgtcggtt acgacaatca
     2761 gcccgatttt gtcaatgccg tctgcaccgt ttccaccact ctggacggca ttgccctgct
     2821 tgccgaactc aaccgtatcg aggctgattt cggacgcgaa cgcagcttcc gcaacgcgcc
     2881 gcgcacattg gatttggaca ttatcgactt tgacggcatc tccagcgacg acacccgact
     2941 caccctgccg catccgcgcg cgcacgaacg cagtttcgtc atccgccctt tggcagaaat
     3001 cctccctgat tttgttttag gaaaacacgg aaaggttgcc gaattgtcaa aacggctggg
     3061 caatcaaggt atccgtcttt tacccgacag gtaattccgc acgcggatgc cgtctgaatg
     3121 ccggtttcag acggcatttt taccctgccg ccaatctgta cgcaaaaaaa atcgcccctt
     3181 gaaaaagggg cgcaaaagga acacaaacca ctaccaaaac tttaaatctg caacactgcc
     3241 tgccgcatac tgtatccgac aggatataaa gccctcacta aatcgtttcg agaaatccaa
     3301 acttcttcat cgccgacaga aaatctgcct ttctccggca ccagctccaa caaaaacggt
     3361 tgaaccgccg tatgcagcct gtccttacac cgccccagct ttctggacaa cgcggacaag
     3421 gcacgcttat agacactatc cttgcggtca agttcccgcg cactgccgac ccagctcaaa
     3481 cgaaggttgc ggctttcatc ctcaatcagc aaaatgcctg ttttgacttc ccccacctcg
     3541 ggctcacatg aaagcgcaat ataatatttg ccgtcgatgt tgacatacgc cgcttcatgc
     3601 acctgacagt tgcagccgga agtggcaagc agcgaataaa cagagtcccg atgcagtttc
     3661 aaaatatcaa gggaaatctg tttgaaaata ctttccggca tttttccctc ctttcgtctg
     3721 ctgcgttgtt tactggattg actgcattat acagacatct cccaatcttg caaatgataa
     3781 ttgttcctat tttttaaaaa atttataaat tattgatgga tatgtgaatt aattttaaga
     3841 tttaaaatct ctttctcaaa aaaacacaaa aatgccgtct gaaacgtgtt cagacggcat
     3901 tggctgcaca gccagtccgt ttaaaatttc acgcccttat gcagcgcgac gatgcccgca
     3961 ctcatattgt gataatccac gctgtcaaag cccgcatcca gcatcatctg tttcaaagtt
     4021 tcctgatcgg ggtgcatacg gatggattcg gcgagatatt gataactctc cgcatctttg
     4081 gcaatcagtt tgcccatgac cggcagcagc ttgaacgaat agaaatcata cgcgccttcc
     4141 aaaggtttgt agattttgga aaactccaat accagcaatg tcccgccggg tttcaaaaca
     4201 cggtacatct ctttcagcgc ggcatcttta tgcgtcatat tccgcaaacc gaacgccacg
     4261 gaaaccaagt tgaaataatt gtcagggaaa ggcagttttt ccgcatcggc aagcgacacg
     4321 ggcaaaatca tgccttcgtt caaaagacgg tcgcgcccga cggtcagcat agaggaatta
     4381 atatcggtca gccaaacctc gccttccttg ccgacccgtt tcgcccaacc gcgcgacaaa
     4441 tcgcccgtac cgccggcaat gtccaacact ttatcgcctt ttttcaggtg cgccgtgttg
     4501 atggtgaaat gcttccacac cctgtgcagc cctgccgaca tcacatcgtt cataatgtcg
     4561 tagtttttgg cgacggagtg gaacacttcc gccacttttc cggctttctc atcttcgttg
     4621 accgtactga atccgaaatg cgttttctgt ccgcccatat tctaaccacc tccctgatgc
     4681 cgccctacgg catcccaaac ttaacaatct gattgctttt caaaacggga agcacccgcc
     4741 gccgccaatt tgtccaaata ttcctgccac atcgcatcgt atccgtatgc cagtctgtgc
     4801 agatacgccc aatcgtaaag accgctgtcg tgcccgtctg aaaaactgat tttcagcgcg
     4861 tactgtccga caggctgcaa atccgcgatt tggacatccg ccttgccggt ctgcaaaaca
     4921 tcctgtccca cgccgtgtcc gcgcacttcc gcactcggcg aatagacgcg taaaaattcg
     4981 gcaggcagat ttttaggttc gtccccataa gtcaaaacca acacggtacg gtttttcaac
     5041 aaacggattt caacaggaat attattcaaa aaacacctcg gttcggcata caatatccgg
     5101 caggcgcagg cgtgccttca aacggcgcgt attttaacag ctttatcccg ataccgatat
     5161 tcaaacccac actttaaacg gcattgatat ggacaatctc gtatgggacg gcgttcccga
     5221 catccgcaca ctcgaccaaa ccatccgcaa acacgcacac ccgctcaacc tgattgtctg
     5281 cctccccgat aatcagattc ccgattttca aaccgcacaa gatgcttcgg acgcggaatg
     5341 ccgtctgaag caccgtttgg atcaggcaac gcagtgcctc cagttcgaca gcatcaacct
     5401 catcgaacac atcctgcccg atgtccgctt ctggctggtt cccccttcac gcacccgccg
     5461 cctgcacgaa cacttccacc acatttcctg gcagaccgaa gccatcccgc aaaccgaaag
     5521 caaacccgac aaaccctggt ttgcacttcc acaaacatcc gaacggcaaa aaccggaaca
     5581 catcctcgtt atcggcgcgg gcatatccgg cgcggcaacc gcccacgcct tagcatcaca
     5641 cggcatttcc gttaccgtat tggaagcccg aaaagccgcc caagccgcca gcggcaaccg
     5701 ccaagggctg ctctacgcca aaatctcgcc gcacgacacc gaacaaaccg aactgctgct
     5761 tgccggctac ggctacacca aacgcctgct cggacacatc ctgcccgaat ccgaaacctg
     5821 gggcggcaac ggcatcatcc acctcaatta cagccgcacc gaacaacaac gcaatcacga
     5881 attgggtttg caaaaacacc ataaccacct ctaccgcagc atcacgcagg cagaagccga
     5941 aaaaatcgcc ggtatcccgc tgaacacgcc ctacgccgaa ccattatgcg gactgttttg
     6001 gcagcacggc gtatggctca atctacccgc attcgtccgc gccctcctcc gccatccgct
     6061 gattggacta cacgaagaca caccgttaac cggtatttcc cacgacggag aaaagtggat
     6121 tgcaagcacg ccaaacggca tcttttctgc cacacacatc gtctactgca ccggcgcgca
     6181 cagcccctgc ctgcccgaaa tcaacctcgc cgccctgccc ctcaggcaaa tacgcggaca
     6241 aaccggcctc acaccgtcca ccccgttttc cgaacaactg cgttgcgccg tttcaggcga
     6301 aagctacatc agcccgtcgt ggcacggact gcactgctac ggcgcgagtt ttattcccaa
     6361 cagcagccat accggatgga acgaagccga agaagcctca aaccgccaag cattggcaca
     6421 ccttaacccc gcccttgccg aatcattgtt tgccgccaac ccaaaccccc aaaaatacca
     6481 agggcacgcc gccatacgct gcgacagccc cgaccacctt cccctagtcg gcgcactcgg
     6541 cgacattgcc gccatgcggc agacctacac caaactcgcg ctggacaaaa actaccgcat
     6601 cgacacccca tgcccatacc tgcctaatgc ctacgtcaac accgcccacg gcacacgcgg
     6661 gcttgccacc gcccccatct gcgccgccgc cgttgcagcc gaaatcctag gcttgcccca
     6721 tcccctctca aaacgcctgc gccacgccct acaccccaac cgcaccatca tccgcgccat
     6781 cgtcagaagg aaggatctaa ccccttaagc ctttgccgca tgccgtcccg accacagaaa
     6841 atcatattca aataaacata ccaatcctga cggtaaaata tcgaaaacac ccataacgtt
     6901 ttcttcccaa aataaggagt aaggaatgcc taacaaattg cctttgctgt ctaagatgaa
     6961 tgttccgtaa cattcgaaga cttccttaac cctgccagcc gtttcggttt ctgtaccccc
     7021 tgttagataa ggaaggaatg aaaaagccct gttgattgta tgaagtaaag ggcgttaatt
     7081 tttgttaatc ttcccttctc aggaactcaa tatataagga tgttaatttt tatttccctt
     7141 ctcgagg
//
LOCUS       DAXHPG010000108         7132 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXHPG010000108 DAXHPG010000000
VERSION     DAXHPG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.108"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..437)
                     /locus_tag="ACFM6F_001559"
     CDS             complement(<1..437)
                     /locus_tag="ACFM6F_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6164950.1"
                     /translation="MLRFFVLEVTARSAADILGIHPNSAALFYRKIRMVINHHLALAA
                     DEVFEGSVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRVYTVVVDNAKSETLLP
                     VIKKKIMPDSIVYTDSLSSCDKLDVSGFIYYRINHSKEFADRQ"
     gene            538..1038
                     /locus_tag="ACFM6F_001560"
                     /pseudo
     CDS             538..1038
                     /locus_tag="ACFM6F_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            1041..1124
                     /locus_tag="ACFM6F_001561"
                     /pseudo
     CDS             1041..1124
                     /locus_tag="ACFM6F_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            1405..1839
                     /locus_tag="ACFM6F_001562"
     CDS             1405..1839
                     /locus_tag="ACFM6F_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164951.1"
                     /translation="MCGLWLLRALTAKLPQQRYAALFITLFFAAVLVFGRHDSMYWYP
                     VLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEKAVRYTRRVTQVWCVFFIINGTL
                     AALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            1846..2361
                     /locus_tag="ACFM6F_001563"
     CDS             1846..2361
                     /locus_tag="ACFM6F_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC6164952.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNRLPPYESDTFGMHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            2381..3859
                     /gene="ubiD"
                     /locus_tag="ACFM6F_001564"
     CDS             2381..3859
                     /gene="ubiD"
                     /locus_tag="ACFM6F_001564"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC6164953.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(3923..6037)
                     /locus_tag="ACFM6F_001565"
     CDS             complement(3923..6037)
                     /locus_tag="ACFM6F_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC6164954.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            6128..7021
                     /locus_tag="ACFM6F_001566"
     CDS             6128..7021
                     /locus_tag="ACFM6F_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC6164955.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVCIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
BASE COUNT         1548 a         1749 c         2130 g         1705 t
ORIGIN      
        1 ttctgacggt ctgcaaattc cttggaatgg ttgatgcggt aataaatgaa accgctcacg
       61 tccaacttgt cgcagctgct cagactatcg gtataaacaa tactgtccgg catgattttc
      121 tttttgatga cagggagtaa cgtttcagac ttggcattat ccaccacaac ggtatagacc
      181 cgtccgttgc gtttcagaat gccgaagaca accacttttc ctgccgcacc gcgaccacgt
      241 ctgcctttac gccgtccgcc gaaatcgctt tcgtccggct cgacagagcc ctcaaaaacc
      301 tcatcggcag ccaaggccaa atgatggttg ataaccatgc ggattttacg gtagaacagt
      361 gctgccgaat tgggatggat acccaaaata tcggcggcag aacgggcggt aacttcgagt
      421 acaaaaaaac ggagcagttc tttctgtact tttttctttc atttgcagtg cgttatcttc
      481 atatttcgag ggtaacatat ctgctaatct agtacagccc ctaaaattta atccactata
      541 gtttatgcct cgcacgacgg ggaaatggcg cgcagtttcg atttgtggtt ggaactgttg
      601 aaatcgcaaa tcgtgtcgcc gacttcgttc ggtttgtcgg tgcacaatgc aacggcgggg
      661 taatggtcga tattgaggcg ggatatgagc gagcagacgg cattggccgt gtgtgccgac
      721 ggtgtggaaa cggcattggc ggaagcggca tcactgttgg aagagggatg cgggtcggtt
      781 ttggttttgg cggcggacga tccgctgccg gaaggatatg ccgtatcggc aacgcgcgcg
      841 ccgtttgctt atgctttggc gatggttctg accaaaggga cgcgttacag tctgacttta
      901 tctgcttcgg acgatatgcc gtctgaagcc ggtatgctgc ccgaggcata ttggagcggt
      961 ttggaatggg tgcgctttct gctgaacggc agtcgggaat gccgccgggt atatcgcaat
     1021 cgtgaatggc tgtggcagcc cgcaagctgc aggctgaaga tttccgcaat gtccgcaccg
     1081 tcaacgacgt ggtacaggcg gtattgaaga ttcaggcggg ctaatcgggt atttctaatt
     1141 atttttcaga cggcatgttt ttatcggatg ccgtctgaaa acatttgtgc ggattcaaaa
     1201 tgaaacccgg ttttttcgga catatttttc tgaccgtatt aagcattgcc tatcccgctt
     1261 tgtggtatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacggca
     1321 aggcgaggca acgctgtact ggtttttgtt aatccactat attacggcag ggaggcgggc
     1381 tggtttgtct ggctggcggc ggcgatgtgc gggttgtggc tgctgcgcgc cctgactgcc
     1441 aaactgcccc aacagcgtta tgcggctttg tttattacac tgttttttgc cgcagtattg
     1501 gtgttcgggc ggcatgattc gatgtattgg tatcccgtcc tcgtcaatct gatgatgttg
     1561 gcggtgttcg gcggcagcct gtttgcaggg cagacggtga ttgaaaaact tgcgcggctg
     1621 caacagcccg acctgcctga aaaagccgtg cgctataccc gccgggtaac gcaggtttgg
     1681 tgcgtatttt ttataatcaa cgggacgctt gccgctttgt tggcacggtt ggggcgatat
     1741 gattggtggg cggtttatac aggcgtgatt gcctatgtat tgatggggat gctgtttgcc
     1801 ggcgaatggc tttaccgcaa actcgttttg aaagtctgaa accttatgta cacttgcact
     1861 tcctgcggcg aaaaacatga agatatgccc gcaatcggct ttaccgcccc cgatccctat
     1921 aatcaactca gtgaagaaga acgcattgct tatcaagcag aatgcgattc tgatttctgc
     1981 atcatccgct atcccgacca aaccgaccgc tttatccgcg cggtgctgcc gattccgatt
     2041 atcgggcatg aggaaacgct ggaatatggc gtgtgggtgt ccgtcagcga aaagagtttt
     2101 aacgactatc aaagccactt tcacgacaat ccggaaaatg tcgtttattt cgggatgatc
     2161 tgcaaccggc tgccccctta cgaatccgac accttcggta tgcattgcaa tgtcgttacc
     2221 caacccgatg gtcaacgccc tttgctgcaa ctccatcaag gcagcgaaca tccgctggta
     2281 cgcgactttt atcacggcat agaatatgcc gaggcacagg caaggatagg ggcggtatcc
     2341 gaatcggatt gctcaggata aaaggacaaa aaggatgaat atgaaataca aagacctgcg
     2401 cgacttcatc gccatgctcg agcagcaggg caaactcaaa cgcatcgcgc accccgtttc
     2461 cccgcatttg gaaatgaccg aaatcgccga ccgcgtgctg cgcgccgaag ggccggcgtt
     2521 gctgtttgaa aacccggtta agcccgacgg tacgcgctac ggttatcctg tgttggcaaa
     2581 cctgttcggc acgcccgaac gtgtggcgat gggcatgggc gcggacagcg tgtccaagct
     2641 gcgtgaaatc gggcagacgc tggcgtattt gaaagaaccc gaaccgccca aaggcatcaa
     2701 agatgcgttt tccaaactgc cgctactgaa agacatttgg agcatggcgc cgaacgtggt
     2761 gaaaaacgcg ccgtgtcagg aaatcgtgtg ggaaggcgaa aacgttgatt tgtataaact
     2821 tccgattcag cattgctggc cggaagacgt tgcgccgctg gtaacatggg gcttgaccgt
     2881 aacgcgcggg ccgcacaaaa aacgccaaaa tctcggcatt taccgccaac aactcatcgg
     2941 caaaaacaag ctgattatgc gctggctgtc gcatcgcggc ggcgcgttgg attatcagga
     3001 attccgcaaa ctcaatcccg atacgccgta tcccgtcgcc gtcgtgctcg gctgcgaccc
     3061 tgccaccatt ttgggcgcgg taacgcccgt tcccgatacc ttgagcgaat accagtttgc
     3121 cggactgctg cgcggttcgc ggacggagct ggtgaaatgt atcggcaacg atttgcaagt
     3181 gcctgcacgc gcagaaatcg tgctggaagg tgtgattcat ccgaacgaaa ccgcgttgga
     3241 aggcccgtac ggcgaccaca cgggctatta caacgagcag gattatttcc ctgtgtttac
     3301 ggtcgaacgc atcaccatgc gcgaaaaccc gatttaccat tcgacctaca caggcaaacc
     3361 gcccgatgag cctgccgttt tgggcgtggc gttgaacgaa gtgttcgtac cgctcttgca
     3421 aaagcagttc cccgaaatca ccgattttta cctgccgccc gaaggctgct cctaccgcat
     3481 ggcggtggtg agcatgaaaa aacagtacgc cggacacgcc aagcgcgtga tgatgggctg
     3541 ctggtcgttc ctgcgccagt ttatgtacac caaattcatc atcgtggtgg atgacgatgt
     3601 gaacgtgcgc gactggaaag aagtcatctg ggcggtaacc acgcgcatgg accccgtgcg
     3661 cgacaccgtt ttggtggaaa acacgcccat cgactatctc gacttcgcca gcccggtcag
     3721 cggactcggc ggcaaaatgg gcttggacgc aaccaacaaa tggccgggag aaaccgaccg
     3781 cgaatgggga cgcgtcatca aaaaagaccc tgcggttacg gctaagattg atgggatttg
     3841 ggaggaattg gggttgtagg ttttacggtc tgccgtctga agcgcggggg cgtttcagac
     3901 ggcatttttg tacgcaggat gtttacggtt ctttaggttt gagccactgc cattgttcca
     3961 gcagggtgtc ttcgaggatt ttctgtttct ctttgcgcga attgatgccg ccggtcagcc
     4021 tgccgtaatc gacggtaatg gacggcttgt ccaccgtacc ggtgattttc aggggaatcg
     4081 gtttgttttt cggatggacg gcgttgcgga taaggacatc ttcagacaat tcctgcgtat
     4141 ccagattggt atagccgttg ctggtaacat agaggctgtc ggagaagagt tcggtatcga
     4201 tgtggcggct gatgccgtct gaaatttcgc tgttgagcgt gaatcggtgg aagggtgtgc
     4261 tgggtgcggc attgtcggca gttttgcccg aaatgccgtt tttcaggata ttgtccatgt
     4321 cgataccgtg ccatgcaccg ttggaaatat tcagcgacag gctgccctga agcgagcgga
     4381 taagctcttt tcgggtttcg ccgctcgcgg tcaggtcgat gaccgcatcg ccgttgccgc
     4441 tgaagctgtg gaagccgaac aggtcttgca gcagcggttg gatttggatg ttgcttgcat
     4501 tctgttgcag gcggtaagtg gcgggacggg tgttggcgat gctgatgccg ccttcggtat
     4561 ggccgccgta aagccctgac ttgaaacggc tgagcgcgat atggcctttg tcggcgtgga
     4621 ggtaggtttc catatcgtcc agttgcaggc cgggaagttg gacttttccg attttcaggt
     4681 gcgcctcgat gtcgccggac agcttggcga gggtgtcggg aaatattttg ccgttttgtt
     4741 gccgcacgtc gtcaagatag ggggtcaggt tcaatttttg cagtgcgacg gcggcttcca
     4801 gatgcggtgc gtcttcatgt gtgtatctga atttcgcggc aacggtttgg cggtcgaatg
     4861 tgccgtttaa ttcggcattc caattttgca gattcggtac ggacagcgaa ccgtcgagcc
     4921 ggctgatgaa acggggttgc ggcaggcggt tgacggtatc ctgaagggtc gatacataca
     4981 ggcgcggcgc gtccagccct ttgttttccg tccatacgag cggcgaattg agggagaagt
     5041 tggtctggtg gcgcggtgtt ttgaagctgc cggagatttc ggcgttgccg atgttggcga
     5101 tgccggagtg aaggttggct ttgtcgagtt tgaacgaacc gtcccattgg gcatattcgc
     5161 cgccggcggt aaatgcgccg ttgacggttt caattttaat gctgttattt ctgagtgcca
     5221 gcgtagggat ttgggcggtc aggtggaggt tgcggaagga agtgtctgca cgcaggccga
     5281 ggccggcggc atccgctccg ccggcgttga agcggacaga agggctgccg gtggtggaaa
     5341 tggtaatgcc gtgtccgtcc agcgaagtgg aagcttcaaa atggaacggt gagattttgg
     5401 gcgtgccgat gccgtctgaa aggaacagcc ccctgctttt ccacgggacg gacagctttc
     5461 cccaaaccag tatgcccgaa ctttcaaacg gctgccccga cgaatcgggg gattgcaggt
     5521 tgaggttgat ttccttcagg ataagctgtt cctgcaggaa attgaggcgg acggtgctgt
     5581 tttcgacgat aatgcggttg actgaggctt ggcgtttttg gctgtcgatc aggtcttgga
     5641 tgttccaaac acctttcccg tcgcgcgtca gggcaagttc cgcactcgaa accacccatt
     5701 tttcaatctg tatctgatcc gaccacaggt ttttccagct caatccgatt ttggtttctt
     5761 ggacggaaac ggcagtctgg tcgccgccgg gttcggtaat ggtcaggttt ttcaggatga
     5821 cggtcggccg gggcaggagc ctgcgctgaa tgtccgcatc aaacgagatt ttccggtgtg
     5881 tgtgtgcaat gctttgttgg aggcggctgc ggatgttttc aggcgtgaag gtgcgatata
     5941 cggaagcgtg cagcccgact gccgccaaaa gcagtatcgt cagcacggcc accgcatatt
     6001 tcagacggta tttgtggaaa accgataata aatccatagc ccgaacaatc tgtaaaacac
     6061 gccacagtat aacacggctt gcaaaaatcc tattttgggc aggccgtttc ttgtgtcagg
     6121 aggaaatatg attattgtca tgagcaggcg tgccgccgaa gcggacattg ccggcgttgt
     6181 cgcctttatc cgcagcagag gtctgcgaga acacatttct cacggcgacg agcgtaccgt
     6241 catcggcgca atcggcgacg atagggtttt gagcgtcagg gaggtgcaga cgctgccgga
     6301 ggtcgagaaa gccgtccgca ttttggatac atggaaaacc gtcagtcggg aaaatcgggc
     6361 ggaagacagc cgcgttgccg ccaaaggcgt ggctttcggc ggcggggaaa ccgtctgtat
     6421 cgccgcagaa ccgtcagtat ggcgcaatgc ggatgcagtg tttctggatc cgtttttcac
     6481 ttcggcaaac ttatacgaca cttcaagtgc ggatgaaggg cgaggacggt gcagacggtt
     6541 ggcggaacag accgcatcgg cgcatggcgc agggaaaccc gtattggtgc gcgtccgcaa
     6601 cgtccgccat gtcgaagcgg ctttgaatgc gggggcggac atcctgtatt tgggcggcgg
     6661 gctgatgtcc gatcttgccg tattgaatga agccggaagc ctgaacattc ccttggtgct
     6721 gtgtaaagac aagcaccatt ctgccgaaga ttggttgaat gccgcagaat atgtcgtctc
     6781 gcgcggcaac cgccacctga ttctgggcga gtcgggcgtg ttgagccata ccaaagacca
     6841 tccctaccgt ttggatgtcg aatccatcgt gaaagtccgc caaatcagcc atctgcccgt
     6901 gattgcgaat attacgggat tatggaaccg cgatatgccg caggaaatcc tgtacggctt
     6961 ggcaaaggcg gcaggcgcgt gcggaatcgt cggaacctgt tttgaaaagt cgggcggata
     7021 gcaggcatac cggcagattc aaaggcggag cagacattcg gacaacatgc cgtctgccgc
     7081 ccgaatagcg gtgccgtctg aagcgcacaa gacggtttca gacggcatat aa
//
LOCUS       DAXHPG010000109         7072 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXHPG010000109 DAXHPG010000000
VERSION     DAXHPG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.109"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            121..1971
                     /gene="ltgA"
                     /locus_tag="ACFM6F_001567"
     CDS             121..1971
                     /gene="ltgA"
                     /locus_tag="ACFM6F_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC6164956.1"
                     /translation="MYLPSMKHSLPLLATLVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYASLFGAPHIPLKQRMGIVPAR"
     gene            2260..2472
                     /gene="rpsU"
                     /locus_tag="ACFM6F_001568"
     CDS             2260..2472
                     /gene="rpsU"
                     /locus_tag="ACFM6F_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC6164957.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2604..3050
                     /locus_tag="ACFM6F_001569"
     CDS             2604..3050
                     /locus_tag="ACFM6F_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC6164958.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(3128..3370)
                     /locus_tag="ACFM6F_001570"
     CDS             complement(3128..3370)
                     /locus_tag="ACFM6F_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164959.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNPFRCIETVLPLPIRYWDV
                     IASALECNYGAANQAIERQRQRAAAVAVHERVAKFL"
     gene            complement(3375..3767)
                     /locus_tag="ACFM6F_001571"
     CDS             complement(3375..3767)
                     /locus_tag="ACFM6F_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC6164960.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3839..4444)
                     /locus_tag="ACFM6F_001572"
     CDS             complement(3839..4444)
                     /locus_tag="ACFM6F_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC6164961.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            complement(4546..6222)
                     /locus_tag="ACFM6F_001573"
     CDS             complement(4546..6222)
                     /locus_tag="ACFM6F_001573"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC6164962.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLACRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAANPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            complement(6383..6997)
                     /locus_tag="ACFM6F_001574"
     CDS             complement(6383..6997)
                     /locus_tag="ACFM6F_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC6164963.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILVENNSFDMLWTVLG"
BASE COUNT         1834 a         1949 c         1883 g         1406 t
ORIGIN      
        1 gtcggaaaca ttccaaaggc ggtgcagttt cggcatataa ttcgggcaaa cgcctgttca
       61 gacggcattt tgtcttttcc aaccctgacc gttcagggtt ccgattctta aggaaatccg
      121 atgtacctac cctctatgaa gcattccctg ccgctgctgg cgaccctagt gcttgccgcg
      181 tgttcttcga caaacacact gccagccggc aagaccccgg cagacaatat agaaactgcc
      241 gacctttcgg caagcgttcc cacccgccct gccgaaccgg aaggaaaaac gctggcagat
      301 tacggcggct acccgtccgc actggatgca gtgaaacaga aaaacgatgc cgccgtcgcc
      361 gcctatttgg aaaacgccgg cgacagtgcg atggcggaaa atgtccgcaa cgagtggctg
      421 aagtctttgg gcgcacgcaa acagtggacg ctgtttgcac aggaatatgc caaactcgaa
      481 ccggcagggc gcgcccaaga agtcgaatgc tacgccgatt cgagccgcaa cgactatacg
      541 cgtgccgccg aactggtcaa aaatacgggc aaactgcctt cgggctgcac caaactgttg
      601 gaacaggcag ccgcatccgg cttgttggac ggcaacgacg cctggaggcg cgtgcgcgga
      661 ctgctggccg gccgccaaac cacagacgca cgcaaccttg ccgccgcatt gggcagcccg
      721 tttgacggcg gtacacaagg ttcgcgcgaa tatgccctgt tgaacgtcat cggcaaagaa
      781 gcacgcaaat cgccgaatgc cgccgccctg ctgtccgaaa tggaaagcgg tttaagcctc
      841 gaacaacgca gtttcgcgtg gggcgtattg gggcattatc agtcgcaaaa cctcaatgtg
      901 cctgccgcct tggactatta cggcaaggtt gccgaccgcc gccaactgac cgacgaccaa
      961 atcgagtggt acgcccgcgc cgccttgcgc gcccgacgtt gggacgagct ggcctccgtt
     1021 atctcgcata tgcccgaaaa actgcaaaaa agcccgacct ggctctactg gctggcacgc
     1081 agccgcgccg caacgggcaa cacgcaagag gcggaaaaac tttacaaaca ggcggcagcg
     1141 acgggcagga atttttatgc ggtgctggca ggggaagagt tggggcgcaa aatcgatacg
     1201 cgcaacaatg ttcccgatgc cgacgcaaac agcgtcctcc gtatggcgga agacggcgcg
     1261 attaagcgtg cgctggtact gttccaaaac agccggaccg ccggcgatgc gaaaatgcgc
     1321 cgtcaggctc aggcggaatg gcgttttgcc acacgcgggt tcgacgaaga caagctgctg
     1381 accgccgcac aaaccgcgtt cgaccacggt ttttacgata tggcggtcaa cagcgcggaa
     1441 cgcaccgacc gcaaactcaa ctacaccttg cgctatattt cgccgtttaa agacacggta
     1501 atccgccacg cgcaaaatgt taatgtcgat ccggcttggg tttacggcct gattcgtcag
     1561 gaaagccgct tcgttatagg cgcgcaatcc cgcgtaggcg cgcaggggct gatgcaggtt
     1621 atgcctgcca ccgcgcgcga aatcgccggc aaaatcggta tggatgccgc acaactttac
     1681 accgccgacg gcaatatccg tatggggacg tggtatatgg cggacaccaa acgccgcctg
     1741 caaaacaacg aagtcctcgc caccgcaggc tataacgccg gtcccggcag ggcgcgccga
     1801 tggcaggcgg acacgcccct cgaaggcgcg gtatatgccg aaaccatccc gttttccgaa
     1861 acgcgcgact atgtcaaaaa agtgatggcc aatgccgcct actacgcctc cctcttcggc
     1921 gcgccgcaca tcccgctcaa acagcgtatg ggcattgtcc ccgcccgctg acgtaccgat
     1981 gccgtctgaa acccgcccgg tctttcagac ggcattttta tcccgaacgg cattgacggc
     2041 gaaccataaa tataagacaa tccgaaaatt gtttttcctg ctttttcaag cagcttgaca
     2101 cggcacaagc cgacccgtta ggaggtgatg tttccgtcac ggcgcgtatc ccgccgccgc
     2161 aaggcacagc gatacggtaa accttcaaca ccgtctgccc taccctttcc accgatatga
     2221 tgggcagatg aaacaaccga atttattaaa ggaaataaaa tgcctgcaat ccgcgtaaaa
     2281 gagaatgaac catttgaagt cgctatgcgc cgtttcaaac gcgccgtaga aaaaaccggc
     2341 ctgctgaccg agctgcgcgc ccgcgaagcc tacgaaaaac cgactaccga acgcaaacgc
     2401 aaaaaagcgg cagccgtaaa acgcctgcaa aaacgcctgc gcagccaaca actgccgccc
     2461 aaaatgtact aaacgttcaa gtacagatta caggtcagcc ctgtgatatg aggacacacc
     2521 gcaagacctg ctctgcggtg tgttttgctt ttcagacggc atcgaaaccc gccgtttcca
     2581 tccgacatcc cagcgaggac atcatgagcc tgaaaatccg ccttaccgaa gacatgaaaa
     2641 ccgcgatgcg cgccaaagac caagtttccc tcggcaccat ccgcctcatc aacgccgccg
     2701 tcaaacagtt tgaagtggac gaacgcaccg aagccgacga tgccaaaatc accgccatcc
     2761 tgaccaaaat ggtcaaacag cgaaaagaca gcgcgaaaat ctacactgaa gccggccgtc
     2821 aggatttggc agacaaagaa aacgccgaaa tcgaggtact gcaccgctac cttccccaaa
     2881 tgctctccgc cggggaaatc cgtactgcgg tcgatgccgc cgttgccgaa accggcgcgg
     2941 caggtatggc ggatatgggc aaagtgatgg gcgtattgaa aacccgcctc gccggcaaag
     3001 ccgatatggg cgaagtcaac aaaatcctga aagccgtgct gaccgcctga ttgcccgaat
     3061 atcggacaaa atgccgtctg aagcccgtat cgcaggttca gacggcattt tcatggtttg
     3121 atgatattta taaaaatttt gcaacacgct catgcacagc aactgccgct gctcgttggc
     3181 gttgccgctc tatcgcttgg ttagctgcac cataattgca ctctaatgct gatgcaataa
     3241 cgtcccaata tcgaatcggc aggggcaaca cggtttcgat acaccgaaac gggttttgcc
     3301 gataaacgga ttccgtttgc gccccatcgg acaaaatgcc gtctgaaaca cgattccgtt
     3361 cagacggcat agatttattt gaccaatttc aagccttttt tggcgggccg gggcgcggtt
     3421 tcggcagagg tgttttcagg cggcgtgtcg gggcgatacg cttccaattc aaaccccata
     3481 ccttcgccgg tctcccgtgc gaaaaggctg aggacgtgtc cgacaggtat ccatatgtcg
     3541 tgcgcctgtc cgccgaagcg ggcggaaaag ctgatccaat cgttgtcgat ttgaaggttt
     3601 tgcgtggcgg tcgcgccgat gttgagcata atttcgttgt cgcggacgta ctgcatgggg
     3661 acacgcgtgt gttcgttgac ccagacaaga atgtgcggcg tgaggctgtt atcgctgcac
     3721 cattcgcaaa gtgcgcggag gatgtagggt ttggtggaag tgggcataat gggttccgtg
     3781 tggtgtgcct gaatgtgaaa atgcctacaa aacggcgggt tttgcaagca ttccgggctt
     3841 atttgcgcat ggctttttcg gcgggtgtca gtgcttcgat aaaggcttcg cgctggaaga
     3901 tgcgctcggc gtatttgagc agcggcgcgg cacttttgcc cagtttgaca tcgtagtggt
     3961 cgagccgcca cagcagcgga gcaagggcga catcaatcat agaaaaatct tcgccgagga
     4021 tgtatttgct tttgctgaac gaaggggcaa gcatggtcag accgttgccg atggcttcgc
     4081 gcgcttttgc ctgttccttg ttggtggcgg cggggttttc taacacttgg acgtggttga
     4141 acaattcttt ttccatacgg tacagcacca gccggccccg gccgcgcata acgggatcgc
     4201 cgggcataag ctgcggatga gggaagcgtt cgtcgatgta ttcgttgatg atgttggact
     4261 cgtgcagcac caaatcgcgc tcgaccagca cgggaacttg gttatacgga ttcatgacgg
     4321 cgaggtcttc gggtttgttg taaatatcga cgtctttgat ttcaaaatcc ataccttttt
     4381 cgtacaaaac gaagcggcag cggtggctga aggggcaggt aatgccggaa tagagggtca
     4441 tcataataat tgtcgctcct gtgtgatgcc tgcaaaacgg ctgatttata aaggtttaat
     4501 cgcgcaatta tacgcgattt ccggcactta atccagaaat tcggctcaat ctgttgtttt
     4561 taatatattt tccccgattt tccgtatcag tgcgaactta ctgtctttgt tgcgcgggcg
     4621 cgcaccctgc cagaccgtct gccagccttg cgacgcatcc gcattttggg gcaagcggac
     4681 gatacggtag cggcattgta catcgccgac gcggtgcggc aatgtgccgt actgcgtcca
     4741 aacaatccgc gtgtgcaggt cgccgccgcc tatgtcgata cactcgatgc cgtctgaaag
     4801 ctcccgtttt aattccgggg aaagcgatgc ctccatactc cggacgaccg gcgcgtggct
     4861 tttcgccgcg tccagccacg gcaggaacag cgtcatcagc aaagcccagg tcagggtaac
     4921 gcctgccgcc cagttggtaa ccgcctgcct gccgcgtatg tttttccggg taatcgccca
     4981 cagccacaag ggtgtgaaca gtacggcaac cgccatcgga atgggatcga tatcaggaac
     5041 ataatacggg ctgaaatagg cggcgcgttc ggcaagcttg gcgggccagc cgtaattcat
     5101 ggcgaaaaag cccgtccaca ggaacacggc aaacagtccg aacgccataa tgccgaacca
     5161 gttgacaaac gccgccgcgc cgcgcctcag gctgtccagt tgcgccgcgc cgaacagggc
     5221 aagcggcggc agcagccaga cgaggttgtc ctgaaaacgc tgcggattgg cggcgagcag
     5281 caccaaaacg gcaagcatcc acacaacgcc caaaatcccc cagtcggtcg aaaacaggcg
     5341 cgtgcggcaa accgtccaaa ccgccagcgg cagcgcgggc agtgcaaacc aaagcaggtt
     5401 tttcagatag taaaacaaac tgaatgccgt ctgaacgtgc cgcacgccgc cgaacgtacc
     5461 gaaaacgtga tcgtcgagcc attgcgcgaa cagcgcgggc tgcgtttttg ccaagagcag
     5521 cgggtaaacg gtcatcagcg gcagggcaaa ggcaagcgag gcgactgccg tcaacatcaa
     5581 acgcctgctt tgccacggac ggaaaaacat cagcactggc aggggcagca tcagggcaaa
     5641 tgctgccgga taagctgctg ccaacgacat cagcgtccaa cccgtaccga gcagaaaaga
     5701 ggcggcaatc acacggcggc acgccagcga gtagccgtgc agcaccagtc cggcggcggc
     5761 aaaggcggcg gcagcgggat tgagaaagtg tacggtcgga atcagcccga tacagccgat
     5821 aaggattaaa acaacgctgc gcccgtggtg tctgcccaaa aagttgaaac cggcaaagcc
     5881 gcaggaagtc agtccgacaa cggcgaaaaa cacgcctgca aagcgtgcgg catcgtatga
     5941 gtcggcagcc cacggcgaca gcaaatgttt gaacgcggcg gcaacccaaa gatacacggg
     6001 cggtatgccg aaatcggttt gaccgaacag atgggcaacc aagggggtgg ggctgcctgc
     6061 cagtgcttcg acggcggtat agacggcagg ttcggcagga ttccataaat catgtgaaaa
     6121 tacacccggc cacaaccagg caaacgccat caacagcagc agccacggct tttcgtgggt
     6181 tttggcgggc gggcgggcat cgggcggggt ataggtcagc ataagcgtga gaaaaaatgg
     6241 acggattgcc aagtgtagca aatattcgca caaaggtcgt gcagagactg cttcagacgg
     6301 catcagacac aaaaagaccg gcaacaaaaa agactgcaca tggcagtctt tgcagatact
     6361 atctttttca taatattttt tcctagccca acacagtcca tagcatatca aaactgttgt
     6421 tttcaaccag gatatatatc cccaatccta aataaacaac agcaacaaac catctgctat
     6481 atttttccaa agtttctcca acagaaggga cttgtgctaa tttttgggca gaaaaaacca
     6541 agagataaat catgactaga aaggtaagta aagccactat caaattcgct aaatttaagg
     6601 tagtaaaata tgggacaaag acaccaatat tgtcagcacc acaacttgca aaagtaatca
     6661 tagcgactag aaaaatcagg tttttattat ctttgcgcaa accctctttg gcaatagcct
     6721 ctccatcaga atctcctaac agcaaaactt tgatgcctag gaaaattgga atcaagccga
     6781 gcaaacctaa aatctcttta ctaggaatat aatctaagac aaatgcaaaa agcaaactta
     6841 gcaatatcag gctaacagag cctagaaatt gtcctaaata gatgttaatg atgtcttttc
     6901 tgctttttct tttggcaaaa aataacatta ggataataag taagtctacg gctgtcccag
     6961 aatacaggat tattgaagta acaacatttt gaatcataaa acatctcatt caaatatatt
     7021 tttaaatgta ttcaaacatt aaaccttgta gatgtcaact tcaaccccgt ca
//
LOCUS       DAXHPG010000110         7009 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXHPG010000110 DAXHPG010000000
VERSION     DAXHPG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.110"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(75..1496)
                     /locus_tag="ACFM6F_001575"
     CDS             complement(75..1496)
                     /locus_tag="ACFM6F_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC6164964.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1854..2213)
                     /locus_tag="ACFM6F_001576"
     CDS             complement(1854..2213)
                     /locus_tag="ACFM6F_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC6164965.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2210..2815)
                     /locus_tag="ACFM6F_001577"
     CDS             complement(2210..2815)
                     /locus_tag="ACFM6F_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFC6164966.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDTSAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2862..3344)
                     /locus_tag="ACFM6F_001578"
     CDS             complement(2862..3344)
                     /locus_tag="ACFM6F_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC6164967.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3548..3645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3855..4955
                     /gene="gcvT"
                     /locus_tag="ACFM6F_001579"
     CDS             3855..4955
                     /gene="gcvT"
                     /locus_tag="ACFM6F_001579"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC6164968.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            5117..5503
                     /gene="gcvH"
                     /locus_tag="ACFM6F_001580"
     CDS             5117..5503
                     /gene="gcvH"
                     /locus_tag="ACFM6F_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC6164969.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDSYGAGW
                     FFKIKPVNPADYDGLLTAEQYAGEVD"
     gene            5655..6902
                     /gene="hemA"
                     /locus_tag="ACFM6F_001581"
     CDS             5655..6902
                     /gene="hemA"
                     /locus_tag="ACFM6F_001581"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC6164970.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSKAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1785 a         1679 c         1966 g         1579 t
ORIGIN      
        1 gtcggaaggg aaaaatattg atttgattat gccggaattt aaaatgccgt ctgaatgttc
       61 agacggcata gcgtttacag cagtttgaaa acgaaaaaga taagggtatg tacgatgaag
      121 acgggtgtca ggaaggcgac cgaccacatc atatagccga agaaagtcgg catcggtacg
      181 ccgcgctgtt cggcaatggc cttgaccatg aagttcggtg cgttgccgat gtaggtcagt
      241 gcgcccatga ataccgaacc catagaaacc gccagcagcg aatgaaacag ggtacccgtc
      301 atcaaggctt gggcatcgcc gcccgccata ttgaaaaaaa cgagataagt gggcgcgtta
      361 tccaagaatg ccgacaatat gccgctcatc caaaaataca tcacattaat cggatgacct
      421 gccgtatcgt gaaccagcga taccaccccg cccagcgcgc ctgcctcgcc tgctttcaga
      481 atgctcagga cgggaaagat ggtgatgaag atgccgagga agagtttgcc cacttcggcg
      541 atgggttcaa agttgaattc gttgcctgcg cggacttgtt tgggcgtgat tgccatagat
      601 acggcggtca atgcaatcag gatgacatcg cggacgaggt tttgcagggc gtaacggctg
      661 ccgaggattt caaatcccgg gtgttcgggt ttccaaaggc cggacattag aaccgcgccg
      721 accacgcccg aaagcaggag gaagttccat ttgccgaaga tggcgatttt ttcgggtttt
      781 tcctgttgtg ccggcgtatc ttgtgcaatg ctttcctgtt tgaagaaacg gttgtcgatg
      841 aaatagaagg cggtcaacag gacagcggtg ctgatcagga cgggggcgaa catatgtttg
      901 accgtccaca tgaaatctac gcctttgagg aagccgagga agagtggggg gtcgcccaaa
      961 ggggtcagac cgccgccgat gtttgcaacc aggaaaatga agaagatgac gatgtgcacg
     1021 cggcgggtac ggttttggtt ggctttcagc agcggacgaa tcatcagcat tgctgcgccg
     1081 gtcgttccca tgatagaggc aagtgccgta ccgacggcaa gcagggcggt gttgagcttg
     1141 ggtgtgccgt tcaagtcgcc ccaaaccaaa atgccgcctg aaatggtgta cagggcaagc
     1201 agcagcagga tgaaggggat gtattcttca acgagtgcgt gtgcgacggt atggataccg
     1261 gcggacgcgc caaaaaccaa actgaacggg atgaggaaga gcaatgtcca aaaggcggtg
     1321 attttgccgt aatggtgatg ccaggtatgc gaaaaaaaca agggacccaa tgcgatagac
     1381 agcaaaatca gggcaaaggg caggctccac agcaggttta ggtttgcgcc gtccaaatct
     1441 gcggcgtaaa ccgatgcggg gaaaagcatt agtgaaaaca ggggtaggtg gcgcatcgtg
     1501 tttcctcgat tcaagcactg ccttgcgcgg cgcgtgggag tgatacaggc accgtgccgc
     1561 ccggacatag gcggactgtg ctgcctgttt gtttttgaaa aaagttttcc gattgattgt
     1621 aaagtaaata atcaggataa accagtttcc caaaccggaa ggcggcggga aggcggattg
     1681 ctgtgcttgg gaatatatgt tctttttgat aaataatttt atttaaaaca aataaatata
     1741 tgtctttaat aaataatcta tcgaaaacga aaaatgaatt tattttaaca tatatttgca
     1801 atgaaacagg tttgcccccc gtttgtttgc ccttatccct ttcagtacgg cattcaagat
     1861 tcgggcctgc gccacatcca tatggcgaca agggaacaaa aaaccgatga aaccgccccg
     1921 acccaccagc gttggggaaa ctgccaaaac attatcaggc aggatgcggt catcatactg
     1981 atggcgaata ttttggcttt gcgcggcact gcgccgtttt gttcccagtt atgaaccatc
     2041 ggaccgaaat agcggtgccg gtgcagccag cggtgaaagc gcggggatgc ctttgcccag
     2101 caggcggcgg agagcagtac gaacggcgtg gtcggcaaca gcggcaaaaa aatgccgatg
     2161 atacccaaca gcagggaaat gcagccgcag gcaattaaaa gataacgtat cattttgaaa
     2221 tatttttctt attgtgcgga taagggcagg atgtgatacc gagttttgcc cagccttcat
     2281 gtcccatttt ttccagcagg gcgatattgc gttcgaatat tgccgaagtg tcgggaaagg
     2341 cttgtgcggc tttggcaatg ctgtcttcgc ggatgaggtg cagcgtcgga tagggagaac
     2401 ggttggtgta gttgccgatg tcgtcgggtt cggtattctg aaagcggaat tggggatgga
     2461 acggtgcgat ttggacgatg ccttccaagc cgttttcgat aacggcggca tcggcaatat
     2521 cgagcatatc gttgaatacg tcgaaatcgg ggaatagggt cgggtgaacc agcagggtgg
     2581 tttccagttc ggtggcgggt gtattgccca gtcgctgcag ttcttcgtcc aagtcttcca
     2641 aaaaactgtc aaggtgtttg gcttcgctga ttgcgatgcg gacaaggttt ttaacgtggg
     2701 gggctttggc aaagggacac aggttcagac cgatgacggc tttttccaac cattgtccgg
     2761 tgtgttcggc aacagcatct ttattttcgg aagtattgat attcattatt gtcatgtaaa
     2821 tgtgtttgca gattgcacgt gcgggaaaat cgggaagggc actattcctt cagcaggtgg
     2881 ttgagcggca gggaggtggt gtgtttgatt tcttttaaaa caaagctcga ttgcgcatct
     2941 tgtacgccgt ggtgggacag gagcgtatcc aaaacaaaat gggaaaacgc gttcatatcg
     3001 gtaaaaaacg cctgaagcag gtagtcggtt tcccctgtca gggcgaagca gctcaagact
     3061 tcaggccatt ttcgaaccga tgcggcaaag tcttcccgcg cgtcttttgc tttgcggatg
     3121 gaaacgcgga taaatgcctg aagccccagg ttgacggatt ccggagacag cagcgcggca
     3181 tattggcgga cgatgccggc atcttccaac tgcttcagac ggcgcaggca cggagaaggc
     3241 gaaagtgcga cacgttcgga cagttcgaca ttggtcagcc tgccgttttc ctggagaacc
     3301 tgtaagattt taatatcggt tttgtctaaa gtgagttggg gcatatttgc gttccgtttt
     3361 aaggaattcg gattgtctgt ccgtatgttt gcggcaatcc gcacagatgg agaccatatt
     3421 aacatataaa aagttatacc gtcatccggg acaaattttg ttttcggaaa atcatgtgaa
     3481 aacagaggcg gtcggtttgc atctctttta agacggcttg cccaaaccgc cgattcaaga
     3541 cataatcggg aaatgtgcag gagagtgtta cacccaacta caatgtaacc accgaaggcg
     3601 cagacaccct taaatcgctc aggtatcagg gactgcacat tgaaacaaac aatctggaga
     3661 gcggcgttgg aataacgtcc accgaagggg agaaggccgt ctgaaccacc attcagacaa
     3721 ccgcgcaaag cagtgagcag actggtttgc catcatgcgg atacggccga aaatctcagg
     3781 ttcaaggaca gatagggtca tccgcgcatt ttgtgcggac ggcatctgaa caaaaaatcc
     3841 ggagaaactt gagaatgatt actctgaaaa ccaccccatt tcatcaagcc catcaagatg
     3901 caggcgcgaa gccggtagat tttgccggct gggagctgcc catccattat ggttcgcaaa
     3961 tcgccgaaca cgaagccgtg cgcaccgacg ccggtatgtt tgacgtatcc catatgcttg
     4021 ttaccgacgt agcaggcgca aatgccaaag cctttttccg caaattgatt gccaacgatg
     4081 tcgccaagct cgcttttgtc ggcaaagccc tttattccgc tttgctcaac gacaacggcg
     4141 gcgtgattga cgacttaatc gtttaccgca ccaacgaagc cgaaacccaa taccgcatcg
     4201 tgtccaacgg agcgactcgc gaaaaagaca cggcgcaatt ccacaaagtc ggacaagagt
     4261 tcggcgttgc ctttaatccg cgctacgacc tcggcatgct cgccgtacaa ggccctaaag
     4321 ccatcgaaaa actcctgacc gtcaaacctg aatgggcgga tgtgattcac ggcctcaaac
     4381 cgttccaagg tgcagacttg ggcaacgatt ggtttgtcgc ccgcaccggc tataccggcg
     4441 aagacggcgt cgaagtgatt ctgccgggca ctgaagccgt cgcattcttc aaagccctgc
     4501 aagcagcagg cgtacaacct tgcggtctcg gcgcgcgcga caccctgcgc atggaagccg
     4561 gcatgaacct ctacggcaac gatatggacg acgataccag cccgctcgaa gcaggcatgg
     4621 gttggaccgt tgatttgaaa gacgaaagcc gcgattttgt cggcaaagct gccttgttgg
     4681 cattgaaaga aaaaggcgtt gccgtcaaac aggtcggttt gctgctcggc aaaggcggca
     4741 tcctgcgcgc gcatatggaa gtgttgaccg acaaaggcaa aggcgaaacc accagcggcg
     4801 tattctcgcc cagcctgaaa caatccatcg ccatcgcccg cgtaccgaaa gactttgacg
     4861 gcgataccgc taaagtgctg atgcgtggca aggaagtgga cgtgcgcgta ttgaagttgc
     4921 cgttcgtgcg taacggtcaa aaacagtttg attaatatcg gttcagacgg cattttcatt
     4981 tcatatgccg tctgaaagca ggttttaatt gttgtccgat acggacgttt gtagaaagca
     5041 ttgaacaagg catctgtgga tattgattca tgcagatgcc gtctgaaaat aacccctatc
     5101 aatggagtat caaaccatga gcaacaacat cccggccgaa ctgaaatacg ttgccagcca
     5161 tgaatggctg cgccttgaag aagacggtac gattaccgtc ggcattaccc accacgcgca
     5221 agagctgttg ggcgacatcg tgttcgtcga gctgcccgaa gtcggcgcga accttgccgc
     5281 tgaagagcaa gccggtgtgg ttgagtctgt aaaagccgcg tccgacgtgt acgcaccgat
     5341 tgcaggcgaa gtcgttgccg tcaacgaaga tttgccaagt gctccggaaa ctgccaacag
     5401 cgattcttac ggcgcaggct ggttcttcaa aatcaaacct gtcaatcctg ccgattacga
     5461 cggtctgctg actgccgaac aatacgcggg cgaagtggat taaaccgccc ggctgcccga
     5521 cggcaaccgc cggacaaacg gaaactgcac cttcagacgg catttttgcg gtcggaggtg
     5581 cagttttttg tccgtgtttt aaggaagcag ttaggctata ataacggcct atattcatct
     5641 ttaccgattt tttcatgcaa cttaccgctg tcggactcaa tcatcaaacc gcacctttaa
     5701 gcatacggga aaagctggcg tttgccgcgg cctgcctgcc cgaagccgtc cgcaatcttg
     5761 cccgaagcaa ggcggcaacg gaggcggtaa tcctttctac ctgcaaccgc accgagcttt
     5821 actgtgtagg cgattcggaa gaaatcatcc gttggctcgc agactatcac agccttccca
     5881 tagaagaaat cagcccctac ctttatactt tggggatgca ggagactgtg cgccatgcct
     5941 tccgcgttgc ctgcggcttg gattcgatgg tgttgggcga gccgcagatt ttaggacaga
     6001 ttaaggatgc ggtcagggtt gctcaagagc aggaaagtat gggtaagaaa ctcaatgccc
     6061 tgttccaaaa aaccttttct gttgctaaag aggtccgtac cgatactgcc gtcggcgaaa
     6121 actcggtttc catggcttcc gcttccgtca aattggcgga acagattttt cccgacatcg
     6181 gcgatttgaa tgtcttgttt atcggcgcag gcgaaatgat tgagctggtt gccacttatt
     6241 ttgccgccaa aagtccccgg ctgatgacgg ttgccaaccg gacgctggcg cgtgcacagg
     6301 agttgtgcga caagctcggt gtcaacgccg aaccgtgcct gctgtccgat ctgcctgcta
     6361 ttctgcacga ttacgacgtg gtggtttctt caacggcgag ccagcttccg atagtcggca
     6421 aaggcatggt cgaacgtgca ttgaaacagc gtcagagtat gccgttgttt atgcttgatt
     6481 tggccgtgcc gcgcgatatt gaagcggaag tcggcgattt gaacgatgcc tatctttata
     6541 cggtggacga tatggtcaac atcgtccaaa gcggcaagga ggcaaggcag aaggccgccg
     6601 ccgccgccga aacgctggta tccgagaagg ttgccgaatt tgtcaggcag cagcagggca
     6661 ggcagagcgt ccccttgatt aaagccttgc gggacgaggg cgagaaagcg cgcaagcagg
     6721 tgttggaaaa tgcgatgaaa cagcttgcca aaggcgcaac ggcagaagag gttttggaac
     6781 ggctgtccgt ccaactgacc aacaagctgc tgcattcgcc gacccaaact ttgaataagg
     6841 cgggggaaga agataaagat ttggttcatg ccgtcgcgca gatttatcat ttggacaaat
     6901 aacggtgcgc cgggaaaaat gccgtctgaa gaggtttcag acggcatttt tttgtgccgc
     6961 ctgacaacat cgtgaaatcc cacattatat cgatgtaatc acaaagtat
//
LOCUS       DAXHPG010000111         6884 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXHPG010000111 DAXHPG010000000
VERSION     DAXHPG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.111"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(132..1478)
                     /gene="mnmE"
                     /locus_tag="ACFM6F_001582"
     CDS             complement(132..1478)
                     /gene="mnmE"
                     /locus_tag="ACFM6F_001582"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC6164971.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1609..1764
                     /locus_tag="ACFM6F_001583"
     CDS             1609..1764
                     /locus_tag="ACFM6F_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164972.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1761..2192
                     /locus_tag="ACFM6F_001584"
     CDS             1761..2192
                     /locus_tag="ACFM6F_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFC6164973.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGS
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2326..6696
                     /gene="app"
                     /locus_tag="ACFM6F_001585"
     CDS             2326..6696
                     /gene="app"
                     /locus_tag="ACFM6F_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC6164974.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYTDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFND
                     DNNGTGKINAKHEHNSLPNRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNRHSLSFHRIQNTDEGAMIVNHNATTTST
                     VTITGNESITQPSGKNINRLNYSKEIAYNGWFGEKDATKTNGRLNLVYQPAAEDRTLL
                     LSGGTNLNGNITQTNGKLFFSGRPTPHAYNHLNNHWSQKEGIPHGEIVWDNDWINRTF
                     KAENFHIQGGQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCV
                     ENTITDDKVIASLTNTDISGNVDLANHAHLNLTGLATLNGNLTAGGDTHYEVTHNATQ
                     NGNLSLVGNAQATFNQAALNGNTSASGNASFNLSDHAAQNGDLTLSGNAKANVSHSAF
                     NGNVSLADKAVFHFENSRLTGQISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITL
                     NSAYRHDAAGAQTGSATDAPRRRSRRSLLSVTPPASAESHFNTLTVNGKLNGQGTFRF
                     MSELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTL
                     QNEHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIA
                     AGRDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQSEAETRPATTA
                     FRRARRARRDLPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAE
                     DRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTF
                     DDGIGNSARLAHGAVFGQYGIDRFYIGISAGAGFSSGSLSDGIGGKIRRRVLHYGIQA
                     RYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQ
                     HISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAA
                     AKGPQLEAQHSAGIKLGYRW"
BASE COUNT         1826 a         1974 c         1690 g         1394 t
ORIGIN      
        1 tgtactgtct gcggcttcgt tgccttgtcc tgatttttgt taatccacta taaaaccaaa
       61 cggatcggat tcccgttttt atgggataac ggaatgttca gccggacacc gccacgatgc
      121 tttcgatccg tttattttcc gatacaaaac ctcgaaaaaa tcacgccgag caggtcgtcc
      181 gccgtaaact cgcccgtgat ttcgccgcat gcgacctgcg ccaagcgcaa gtgttcggca
      241 aacagctcga tttgatggtt gccgcacaat gccgccagcg acaattcttc ctgcgctgct
      301 ttgagtgcgt tgacgtgccg cgtccgcgcc aaaaacagcc cttcgccctc gccctgccat
      361 ccggcctcgc gcaacaacgt ccgtttcagc gcgtccaagc cgtcgccggt tttcgccgac
      421 agcgcgatga cggtttccgc gcccgtaccg aacccgcctg ccgcgtgtgc gtgcaaatcg
      481 gatttgctgt ggatttcgat gcgtttcaac tccggcggca acgcgtccaa aatcgcccgt
      541 gtcttgtcgt tcacgccttc gcgcggatcg accaacacca gcgcgacatc ggcttcggat
      601 acggctttgc ggctgcgttc gatgccgatg cgttcgacca cgtcgtccgt ctcgcgcaaa
      661 cctgccgtat cgacaatatg caccggaacg ccgtcaatca ggatacgttc cctgaccgcg
      721 tcgcgcgtcg ttccggcaat atcggtaaca atcgccactt cgtcgcccgc cagcgcgttc
      781 aacaggctgg atttgccgac attcggcgcg ccgaccaata cgacattcag accttcgcgc
      841 aaaatcgcgc cctgctgcgc gttggcaagc acatcatcca cggcgcggcg caagccgtcc
      901 agtttgccgc gtgcgtctgc cgcttcgaga aaatcaatgt cttcctcggg aaaatctaac
      961 gtcgcttcga ccagcatccg caaggtaatc aagtcttcga ccagaccgtg tatccgccgc
     1021 gaaaaatcgc ctttgagcga gcgcaacgcc agacgcgccg ccgaacggct ggatgcgtca
     1081 atcaaatccg ccacgccttc cgcctgtgcc aagtccagtt tgtcgtttaa aaacgcacgc
     1141 ttcgtaaact cgcccggttc ggcaaggcgc gcgcccaatt ccaaacagcg gttcagcagc
     1201 atatccatca ctaccggccc gccgtgtccc tgaagctcga taacatcttc gcccgtaaaa
     1261 cttgccggtg cggcaaaaaa aagcaaaagc ccgctgtcga ttgcctgtcc gtccgcgtcc
     1321 gtaaaatcag cataggttgc cacgcgcggc ttgggcgttt tcccgcacaa agcctgcgcc
     1381 atcggcagca ggtttttccc cgatatgcgt atcacgccca cgccgccgcg tccgggcgcg
     1441 gtagcgactg ccgcaatcgt tggaacgtta tccgacataa aacccccgaa aattcaaaac
     1501 agccgcgatt atagcaaatg ccgtctgaag tccgacggtt tggctttcag acggcataaa
     1561 accgcaaaaa tgcttgataa atccgtccgc ctgacctaat ataaccatat ggaaaaacga
     1621 aacacatacg ccttcctgct cggtataggt tcgctgttgg gcctgttcca tcccgcaaaa
     1681 accgccatcc gccccaatcc cgccgacgat ctcaaaaaca tcggcggcga ttttcaacgc
     1741 gccatagaga aagcgcgcaa atgaccgaaa acgcacagga caaggcgcgg caggctgtcg
     1801 aaaccgtcgt caaatccccg gagcttgtcg agcaaatcct gtccgacgag tacgtgcaaa
     1861 taatgatagc ccggcgtttc cattcgggat cgttgccgcc gccgtccgac ttggcgcaat
     1921 acaacgacat tatcagcaac ggggcagacc gcattatggc aatggcggaa aaagaacaag
     1981 ccgtccggca cgaaaccata cggcaagacc aaaccttcaa caggcgcggg caactgtacg
     2041 gcttcatcag cgtcatcctg atactgcttt ttgccgtctt cctcgtatgg agcggctacc
     2101 ccgcaaccgc cgcctccctt gccggcggca cagtggttgc cttggcgggt gctttcgtga
     2161 ttggaagaag ccgagaccaa ggcaaaaatt aatgacaaat cctagggcgt gcttcatatc
     2221 cgcccgaacg ccgaaccgca catataggca catcccgcgc gccgcccgga agcggaagcc
     2281 gcgccctccc aaacaaaccc gaatcccgtc agataaggaa aaataatgaa aacaaccgac
     2341 aaacggacaa ccgaaacaca ccgcaaagcc ccgaaaaccg gtcgcatccg cttctcgcct
     2401 gcttacttag ccatatgcct gtcgttcggc attcttcccc aagcctgggc gggacacact
     2461 tatttcggca tcaactacca atactatcgc gactttgccg aaaataaagg caagtttgca
     2521 gtcggggcga aagatattga ggtttacaac aaaaaagggg agttggtcgg caaatcaatg
     2581 acaaaagccc cgatgattga tttttctgtg gtgtcgcgta acggcgtggc ggcattggtg
     2641 ggcgatcaat atattgtgag cgtggcacat aacggcggct ataacaacgt tgattttggt
     2701 gcggagggaa gcaatcccga tcagcaccgc ttttcttacc aaattgtgaa acggaataat
     2761 tataaagcag ggactaacgg ccatccttat ggcggcgatt atcatatgcc gcgtttgcat
     2821 aaatttgtca cagatgcaga acctgttgag atgaccagtt atatggatgg gtggaaatat
     2881 accgatcaaa ataattaccc tgaccgtgtt cgaatcggag caggcagaca atattggcgg
     2941 tctgatgaag atgaacccaa taaccgcgaa agttcatatc atattgcaag tgcgtattct
     3001 tggctcgttg gtggcaatac ctttgcacaa aatggatcag gtggtggcac agtcaactta
     3061 ggtagtgaaa aaattaaaca tagcccatat ggttttttac caacaggagg ctcatttggc
     3121 gacagtggct caccaatgtt tatctatgat gcccaaaagc aaaagtggtt aattaatggg
     3181 gtattgcaaa cgggcaaccc ctatatagga aaaagcaatg gcttccaact agttcgtaaa
     3241 gattggttct atgatgaaat ctttgctgga gatacccatt cagtattcta cgaaccacgt
     3301 caaaatggga aatactcttt taacgacgat aataatggca caggaaaaat caatgccaaa
     3361 catgaacaca attctctgcc taatagatta aaaacacgaa ccgttcaatt gtttaatgtt
     3421 tctttatccg agacagcaag agaacctgtt tatcatgctg caggtggtgt caacagttat
     3481 cgacccagac tgaataatgg agaaaatatt tcctttattg acgaaggaaa aggcgaattg
     3541 atacttacca gcaacatcaa tcaaggtgct ggaggattat atttccaagg agattttacg
     3601 gtctcgcctg aaaacaacga aacgtggcaa ggtgcgggcg ttcatatcag tgaagacagt
     3661 accgttactt ggaaagtaaa cggcgtggca aacgaccgcc tgtccaaaat cggcaaaggc
     3721 acgctgcacg ttcaagccaa aggggaaaac caaggctcga tcagcgtggg cgacggtaca
     3781 gtcattttgg atcagcaggc agacgataaa ggcaaaaaac aagcctttag tgaaatcggc
     3841 ttggtcagcg gcaggggtac ggtgcaactg aatgccgata atcagttcaa ccccgacaaa
     3901 ctctatttcg gctttcgcgg cggacgtttg gatttaaaca ggcattcgct ttcgttccac
     3961 cgtattcaaa ataccgatga aggggcgatg attgtcaatc ataatgccac aacaacatcc
     4021 accgttacca ttacagggaa tgaaagtatt acacaaccga gtggtaagaa tatcaataga
     4081 cttaattaca gcaaagaaat tgcctacaac ggttggtttg gcgagaaaga tgcgaccaaa
     4141 acgaatgggc ggctcaacct tgtttaccag cccgccgcag aagaccgcac cctgctgctt
     4201 tccggcggaa caaatttaaa cggtaacatc acgcaaacaa acggcaaact gtttttcagc
     4261 ggcagaccaa caccgcacgc ctacaatcat ttaaacaacc attggtcgca aaaagagggc
     4321 attccgcacg gagaaatcgt gtgggacaac gactggatta accgcacgtt taaagcggaa
     4381 aatttccata ttcagggcgg gcaggcggtg atttcccgca atgttgccaa agtggaaggc
     4441 gattggcatt tgagcaatca cgcccaagca gttttcggtg tcgcaccgca tcaaagccac
     4501 acaatctgta cacgttcgga ctggacgggt ctgacaaatt gtgtcgaaaa caccattacc
     4561 gacgataaag tgattgcttc attgaccaat accgacatca gcggcaatgt cgatcttgcc
     4621 aatcacgctc atttaaatct cacagggctt gccacactca acggcaatct gaccgcaggc
     4681 ggagacacgc actatgaggt tacgcacaac gccacccaaa acggcaacct tagcctcgtg
     4741 ggcaatgccc aagcaacatt taatcaagcc gcattaaacg gcaacacatc ggcttcgggc
     4801 aatgcttcat ttaatctaag cgaccacgcc gcacaaaacg gcgatctgac gctttccggc
     4861 aacgctaagg caaacgtaag ccattccgca ttcaacggca atgtctctct agccgataag
     4921 gcagtattcc attttgaaaa cagccgcctt accggacaaa tcagcggcgg caaggatacg
     4981 gcattacact taaaagacag cgaatggacg ctgccgtcag gcacggaatt aggcaattta
     5041 aaccttgaca acgccaccat tacactcaat tccgcctatc gccacgatgc ggcaggggcg
     5101 caaaccggca gtgcgacaga tgcgccgcgc cgccgttcgc gccgttccct attatccgtt
     5161 acacctccgg cttcggcaga atcccatttc aacacgctga cggtaaacgg caaattgaac
     5221 ggtcagggaa cattccgctt tatgtcggaa ctcttcggct accgcagcga caaattgaag
     5281 ctggcggaaa gttccgaagg cacttacacc ttggcggtca acaataccgg caacgaacct
     5341 gcaagcctcg aacaattgac ggtagtggaa ggaaaagaca acaaaccgct gtccgaaaac
     5401 cttaatttca ccctgcaaaa cgaacacgtc gatgccggcg cgtggcgtta ccaactcatc
     5461 cgcaaagacg gcgagttccg cctgcataat ccggtcaaag aacaagagct ttccgacaaa
     5521 ctcggcaagg cagaagccaa aaaacaggcg gaaaaagaca acgcgcaaag ccttgacgcg
     5581 ctgattgcgg ccgggcgcga tgccgtcgaa aagacagaaa gcgttgccga accggcccgg
     5641 caggcaggcg gggaaaatgt cggcattatg caggcggagg aagagaaaaa acgggtgcag
     5701 gcggataaag acaccgcctt ggcgaaacag agcgaagcgg aaacccggcc ggctaccacc
     5761 gccttccgcc gcgcccgccg cgcccgccgg gatttgccgc aaccgcaacc ccaaccgcag
     5821 ccccaaccgc agcgcgacct gatcagccgt tatgccaata gcggtttgag tgaattttcc
     5881 gccacgctca acagcgtttt cgccgtacag gacgaattag accgcgtatt tgccgaagac
     5941 cgccgcaacg ccgtttggac aagcggcatc cgggacacca aacactaccg ttcgcaagat
     6001 ttccgcgcct accgccaaca aaccgacctg cgccaaatcg gtatgcagaa aaacctcggc
     6061 agcgggcgcg tcggcatcct gttttcgcac aaccggaccg aaaacacctt cgacgacggc
     6121 atcggcaact cggcacggct tgcccacggc gccgttttcg ggcaatacgg catcgacagg
     6181 ttctacatcg gcatcagcgc gggcgcgggt tttagcagcg gcagcctttc agacggcatc
     6241 ggaggcaaaa tccgccgccg cgtgctgcat tacggcattc aggcacgata ccgcgccggt
     6301 ttcggcggat tcggcatcga accgcacatc ggcgcaacgc gctatttcgt ccaaaaagcg
     6361 gattaccgct acgaaaacgt caatatcgcc acccccggcc ttgcattcaa ccgctaccgc
     6421 gcgggcatta aggcagatta ttcattcaaa ccggcgcaac acatttccat cacgccttat
     6481 ttgagcctgt cctataccga tgccgcttcg ggcaaagtcc gaacacgcgt caataccgcc
     6541 gtattggctc aggatttcgg caaaacccgc agtgcggaat ggggcgtaaa cgccgaaatc
     6601 aaaggtttca cgctgtccct ccacgctgcc gccgccaaag gcccgcaact ggaagcgcaa
     6661 cacagcgcgg gcatcaaatt aggctaccgc tggtaaccgc cggatatgcc gaaaggggtc
     6721 tgacgatgcc accgtgcgct gtcaaaccct ttttctgccg ccgccttgtg cctgctccat
     6781 tgtctgataa ccgtcaaatt gatttttagc ccatgtttgg tgggtcgggg aaatctatat
     6841 cttcgtccgt gccgaaatag tctgagacct ttgcaaaatt cctt
//
LOCUS       DAXHPG010000112         6819 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXHPG010000112 DAXHPG010000000
VERSION     DAXHPG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.112"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            188..1372
                     /gene="ubiM"
                     /locus_tag="ACFM6F_001586"
     CDS             188..1372
                     /gene="ubiM"
                     /locus_tag="ACFM6F_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC6164975.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKARLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(1629..1784)
                     /gene="rpmG"
                     /locus_tag="ACFM6F_001587"
     CDS             complement(1629..1784)
                     /gene="rpmG"
                     /locus_tag="ACFM6F_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC6164976.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1816..2049)
                     /gene="rpmB"
                     /locus_tag="ACFM6F_001588"
     CDS             complement(1816..2049)
                     /gene="rpmB"
                     /locus_tag="ACFM6F_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC6164977.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2299..3825)
                     /gene="farB"
                     /locus_tag="ACFM6F_001589"
     CDS             complement(2299..3825)
                     /gene="farB"
                     /locus_tag="ACFM6F_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC6164978.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHSSGGGGH
                     "
     gene            complement(3849..5021)
                     /gene="farA"
                     /locus_tag="ACFM6F_001590"
     CDS             complement(3849..5021)
                     /gene="farA"
                     /locus_tag="ACFM6F_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC6164979.1"
                     /translation="MATHGDEVKIQNRQEADSAKAKRKRRLTALTLLFALAAAAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      5250..5294
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5298..5771
                     /gene="queF"
                     /locus_tag="ACFM6F_001591"
     CDS             5298..5771
                     /gene="queF"
                     /locus_tag="ACFM6F_001591"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC6164980.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDSQ"
     gene            5847..6533
                     /locus_tag="ACFM6F_001592"
     CDS             5847..6533
                     /locus_tag="ACFM6F_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC6164981.1"
                     /translation="MYAFTDAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIALVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT         1677 a         1960 c         1603 g         1579 t
ORIGIN      
        1 ataccccaca cccgatttga tacacattaa gctgaaagta aaaatccgca tacaccctcc
       61 cctgcatatt tcttcaacag tgggttttga tataatcgcc tatctgttac agatagttca
      121 aacggcattc aaacccttac aaataccgtc tgaaatcatc ggcatccgtc tatgcaaagg
      181 atattttatg agtttacaca gcgacatcct cgtcgtcggc gcaggcccgg cgggtttaag
      241 ttttgcagcc gaacttgccg gcagcggttt gaaagttacc ctgatcgaac gcagcccact
      301 gactgttctg caaaatcctc catatgacgg acgcgaaatc gccctgaccc atttttcccg
      361 cgaaatcatg cagcgtttgg gtatgtggga caaaattccc gaaaacgaaa tctacccttt
      421 gcgcgatgcc aaagtgctga acggacgttc cgactaccag ctccactttc cccaaccgac
      481 tgaagcgcgc ggcgaacctg ccgactgttt gggctatctg atttccaacc acaatatccg
      541 ccgcgccgcc tatgaagtcg tatcacaact cgacaatgtc agcattctga ccgatacggt
      601 tgtcaaagaa gtcaaaacat ctgacaacga agcacaagtc attttggaaa acggcaaaat
      661 cctgactgca cgcctgcttt tggcggcaga cagccgcttc tcgcaaaccc gtcgacaact
      721 cggtatctcc tccgatatgc acgattacag ccgaaccatg ttcgtctgcc gtatgaaaca
      781 taccctttcc aaccaacata ccgcttacga atgcttccat tacggacgca ccatcgcact
      841 gctgccgctg gaagagcacc tgaccaatac cgtcattacc gtcgataccg ataaaatcaa
      901 cagcgttcaa aacctttcgc ccgaagaatt ggccgcaagc gtaaaagaac agctcaaggc
      961 caggctcggc gatatggaac tggtcagcag catccaccat tacccccttg tcggtatgat
     1021 tgccaaacgt ttctacggca aacgcagcgc gctgatcggc gatgcggctg tcggtatgca
     1081 ccccgttacc gcacacggtt tcaacttggg tctgtccagc gcagatattt tggcaaaact
     1141 gatactcgaa gccgagcagc gcggtcagga catcggcgcg gcttccttgt tggaaaaata
     1201 cagcagcaaa catatgctgc acgcccatcc gctttatcac gggaccaata tgatgctgaa
     1261 gctgtttacc aacgaaaccg cgccggcaaa actgctgcgc ggcttggtat tgcgtgccgg
     1321 caacaatttc ccgccgctga aaaagctgat taccaaacaa ctgaccggtt gaaaactgcc
     1381 gaaatgccgt ctgaaacgtt tcagacggca tttttgtatt atagtggatt aacaaaaatc
     1441 aggacaaggc gacgaagccg cagacagtac aaatacactt ggtgcttcag caccttagag
     1501 aatcgttctc tatccttggc agacggtttg cagcagattc gcatacggca gatgtttcaa
     1561 gcggaaaaga aacacgataa aaataaaaaa gcctccgaac agtcggaggc tttgctttca
     1621 aactgaaatt atttcagttt ggtttctttg tacactacgt gtttgcgggc aaccggatcg
     1681 aattttttaa tttccagttt gccgggcata gtgcgtttgt ttttggtagt ggtgtagaag
     1741 tgaccagtac ctgcactgga ttccagtttg attttatcgc gcattgcagt aatccttaat
     1801 taaatagtgt ttaaattaag cttcgccgcg agcacgcaaa tcagccaata cgacatcaat
     1861 gcctactttg tcgatggtac gcagagcggc gttagaaacg cgcaggcgaa cccagcggtt
     1921 ttcactttct acccaaaaac gacgtgattg caagttgggc aaaaaacggc gtttggtttt
     1981 gttgttggcg tgcgatacgt tgttgccgga catcgggcgt ttgccggtca ctttgcaaac
     2041 tcgtgccata atcagtctcc aaacttctaa aatagtaaaa cgcgatttat accacgaaaa
     2101 acagtgttag ttcaagtgtt ttaaagaaaa acggctgttt tgcgcggata atccttccga
     2161 ttgtcgcatc tgtggaacgt ttgcaggtgc gaaacagatg gggcgcgatt atagcggatt
     2221 tcgggaactg ctgcattcaa aatgccgtcc gaacatattt ccagtatttt cagacggcat
     2281 ttcaagtttt caaatccctc aatgtccacc gccgccactg ctgtggaacg gcggttttgc
     2341 cagccatatg acgggtatca tgataatgaa taacaagctg cctgccataa agatttcatt
     2401 cgatccgatg atgaagccct gctgggtaat ggtattgttg atgataccta gggtttgaat
     2461 gtcggaaatg ccttgttggg acaaatgcgc cgccgtttcg tgcaatgttg cggaataggg
     2521 cgtgatgtgt tcggcaaagc gcgtgtggtg caacgcttcg cgccgttccc acagggtgct
     2581 gacgacggat acgccgacac cgcccatcag cacgcgcagg aaattcgaca ggctgccggc
     2641 ggcggcaatc tgcccgccct tcatgtgcga cagtgtgatg gtggtcagcg gcaggaagaa
     2701 catggcaacg cctgccccct gccaaaactg cggccagatg acgttgccga tatccatgtc
     2761 ggcataaaaa tcggtgcgcc aatagaaagt aaaggcaaag gtcaggaagc tggcggttac
     2821 gagcaggcgc atatcgattt tattgccgaa cctgccgatt aacggagaca ggaagacggg
     2881 caggatgccg acgggtgcgg cggcaagccc cgcccacgtg gaggtatagc ccaggttggt
     2941 ctgcaacact aacggcagca aggtcagcgt ccccatatac accataaaac ccaatgatgt
     3001 ggcaattacg ccgacggtaa aattccgatc tttaaacagc gataaatcga caatcggata
     3061 tttttctccc aattcccaaa caataaaata cgacaaacac accagtgcga ctacgcccaa
     3121 ggtaatgatt tctccagagg cgaaccagtc gagttcctta cccctgtcca gcatcatctg
     3181 taacgcgccg ataccgacta ccatcaatgt aagcccgaca tagtcggtcg gcattttaac
     3241 ggtttccgtt tcccgatatt tcaaatgttt ccatgtaatc catgccgata tgataccgat
     3301 agggatatta atgaagaaaa tccaacccca atgccagttt ccggaaatcc aaccgccgag
     3361 tatcggcccg agaacagggg cgacaacgac ggtcattgcc cacaatgcca gtgccagcgt
     3421 ccgttttgcg ggcggatagg atgccattaa caggctttgc gacaggggaa tcagcggccc
     3481 ggcgataaag ccctgcaaga tgcggaaaac aaccagcgac tgaaggttgg gggcaatacc
     3541 gcacagccac gatgtgatga cgaaaccgac agcggcggcg gtaaacaatt tgacctcgcc
     3601 gatacgtttt gccaaaaagc ccgttaacgg cacggaaacg gcgtttgcca cagcaaaaga
     3661 agtaatgacc cacgtcccct gcgtggttgc cgcaccgagg ttgccggcga tgacgggaac
     3721 ggcgacattg gcgatagtcg tatctaaaac ttccataaat acggcaagcc ccaaagacag
     3781 cgtaacccac gccaatgccg cacccttaag cggtggataa tccattgtgt cctccgtttc
     3841 agacggcatc agggcgcgga ttgcccgagg atttcatcga ccgtccgatc gacttccgac
     3901 cagtcggtac tttccatttc cggcaatgcc gcacccggcg tttttgaaac aggcgcgcct
     3961 gcggcggaag tatccacttt aaccgtcatc gacaaaccga tacgcaacgg atgcctgtcc
     4021 acatcttcgc ggttcagcac gatacggacg gggacgcgct gcaccacttt aatccagttg
     4081 cccgttgcgt tttgcgccgg aatcagcgaa aacgcgctgc ccgtacctgc cgaaaaacct
     4141 gccaccctgc cgcgataaac aatttgtttg ccgtacaaat cggacaccag ctcggcaggc
     4201 tgtccgattt tcatatgccg caactgcgtc tctttaaaat tagcatccac ccacacatcc
     4261 gacagcggca ccaccgccat cagcggcgcg cctgccgcca cctgctgccc gacctgcacc
     4321 gaacgcttcg ccacctgacc gtccgccggc gcgcggattt gcgtccgctg aaggttcaac
     4381 cacgcatctt tcaacctgcc gattgccgtc tgaaccgccg gctgttcgcg caaagaaacc
     4441 tgaccgccca aagccgcacg tgccgaagat tcttccgcca aagccgcctt aactgccgcc
     4501 tgagcctgag acactgccgc acgggcgtgt gccagctctt cggcggacac cgcgcccgat
     4561 tccgccaaag cagaccggcg gcgcaaatca tcctgtgcgc gtgccaaatc cgcccggcgc
     4621 aaggcaacct gcgcccccgc ctgcgaagtg gcggcatttt gccggcggtt ttgccgcacc
     4681 gcctgaacca gctcgttttt tgcccgctcg taagccagca catcattatc gtcgtccaat
     4741 accgccagca cgtcgccttt tttcacggca tccgtatcgt cgtgcaaaac cttccgcacc
     4801 gtaccgccct tttgcggcgt aacctgaacc acgcgtccgg caacataagc gtcttccgtt
     4861 tcctcttcgt gctgccacca taaaaaaaac gccgacccgg cggctgcggc ggcaagcgcg
     4921 aacagcagcg tcaatgccgt caggcggcgt ttgcgtttgg cttttgcgct atcggcttct
     4981 tgtctgtttt gaattttcac ttcgtctccg tgtgttgcca ttattccaat tccgaatatt
     5041 tttaatacga tataaataaa gccgatcaaa ggcagctcgg attctatacc ctggcaataa
     5101 cccaatcaag ttaatgattt agaatgaata ttcaaataaa ccgccacacc cgcgttccgc
     5161 aaaattaatt tatgttcaaa aataatcgga taagcgcgcc gtaccgggcg gggacgctac
     5221 acagataccg ccgcttatat tacaatcgtc gccccgtggt tcgaaaacct cccacactaa
     5281 aaaactaagg aaaccctatg tcccgcaata ccgaagagct gcaaggcatc tcgcttttgg
     5341 gtaatcaaaa aacccgatat ccgaccggct acgcgcctga aattctcgaa gcattcgaca
     5401 acaaacatcc cgacaacgac tatttcgtca aattcgtctg cccagagttc accagcctct
     5461 gccccatgac cgggcagccc gacttcgcca ccatctacat ccgctacatt ccgcacatca
     5521 aaatggtgga aagcaaatcc ctgaaactct acctcttcag cttccgcaac cacggcgatt
     5581 ttcatgaaga ctgcgtcaac atcatcatga aagacctcat tgccctgatg gatccgaaat
     5641 acatcgaagt attcggcgag ttcacaccgc gcggcggcat cgccattcat ccgtttgcca
     5701 actacggcaa agcaggcacc gagtttgaag cattggcgcg taaacgcttg ttcgaacacg
     5761 acagccaata atccgtcctt cccactttca gacggcattc tttcgatacg gtatgccgtc
     5821 tgaacgcctt tccgaaaggt ttttttatgt acgcatttac cgacgcacag caacagaagg
     5881 cactcttccg gctggtgctt tttcatatcc tcatcatcgc cgccagcaac tatctggtgc
     5941 agttcccctt ccaaattttc ggcatccaca ccacttgggg cgcgttttcc tttcccttca
     6001 tcttccttgc caccgacctg accgtccgca ttttcggttc gcacttggca cggcggatta
     6061 tcttttgggt catgttcccc gcccttttgc tttcctatgt cttttccgtt ttgttccaca
     6121 acggcagttg gacgggcttg ggcgcactgt ccgaattcaa cacctttgtc ggacgcatcg
     6181 cgctggccag ctttgccgcc tacgcgctcg gacaaatcct tgatattttt gtgttcaaca
     6241 aattacgccg tctgaaagcg tggtggattg ccccgaccgc atcaaccgtc atcggcaacg
     6301 ctttggatac gctggtattt ttcgccgttg ccttttacgc cagcagcgat ggatttatgg
     6361 cggcaaactg gcagggcatc gccttggtcg attacctgtt caaactcacc gtctgcggtc
     6421 tgtttttcct gcccgcctac ggcgtgattc tgaatctgct gacgaaaaaa ctgacgaccc
     6481 tgcaaaccaa acaggcgcaa gaccgccccg cgccctcgct gcaaaatccg taaaacagcc
     6541 ctgccggaaa tcgggacggt tgggttttct tttatttgca aacactgtcg gaacggataa
     6601 atccggattc ccgcccactt cgtcattccc gcgaaagcgg gaatccggtg cgttgggttt
     6661 cagtcatttc cgataaactc ctgccgcatt gtatttctag attcccgctt tcgcgggaat
     6721 gacggcggat gggtttccgt accgtggtgt ccggattccc gacggtacgg gaatgacggc
     6781 agtgcttcct tggcttagtt ttattttaat cgactgtat
//
LOCUS       DAXHPG010000113         6799 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXHPG010000113 DAXHPG010000000
VERSION     DAXHPG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.113"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            175..1233
                     /gene="alr"
                     /locus_tag="ACFM6F_001593"
     CDS             175..1233
                     /gene="alr"
                     /locus_tag="ACFM6F_001593"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC6164982.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGITEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1775..3130)
                     /locus_tag="ACFM6F_001594"
     CDS             complement(1775..3130)
                     /locus_tag="ACFM6F_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC6164983.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVTTAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETVKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(3145..3417)
                     /locus_tag="ACFM6F_001595"
     CDS             complement(3145..3417)
                     /locus_tag="ACFM6F_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC6164984.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENQINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3543..4280)
                     /locus_tag="ACFM6F_001596"
     CDS             complement(3543..4280)
                     /locus_tag="ACFM6F_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC6164985.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVVAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4561..5460)
                     /gene="prmB"
                     /locus_tag="ACFM6F_001597"
     CDS             complement(4561..5460)
                     /gene="prmB"
                     /locus_tag="ACFM6F_001597"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6164986.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5605..6198
                     /locus_tag="ACFM6F_001598"
     CDS             5605..6198
                     /locus_tag="ACFM6F_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164987.1"
                     /translation="MADGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     KNSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            6310..>6799
                     /locus_tag="ACFM6F_001599"
     CDS             6310..>6799
                     /locus_tag="ACFM6F_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC6164988.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFGAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANPDAVSTCFKADKGAEAVNNDTEHNAT
                     YFS"
BASE COUNT         1866 a         1683 c         1554 g         1696 t
ORIGIN      
        1 tttgggaaag cgattatatc aggaaaagca aaccgccttc ctacctgaaa actgctgctt
       61 cggcttgaag acacaagggt ctttaatatt ttaaaagcct tgccgttgga ttataattcc
      121 ctaattgatt tcttaatttt gctaataaac acttgcttgg taaggaatga atttatgcgc
      181 cctttgaacg tgcagatcag gttgggcaac cttaggcaca attatcggat tttaaaggaa
      241 atgcacggag gcaagctgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc
      301 agatgtgctt tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgatgaa
      361 ggaatcaggc tgcgggagag cggcattacc catccgattg tccttttgga aggcgtattt
      421 gaagcatcag agtacgaagc ggtcgaacaa tactcgctct ggccggcagt cggaaaccaa
      481 tggcaacttg aggctttgct ttcccgccat tggaaaaaac ctgtcaaggt ctggttgaaa
      541 atggattcgg gaatgcaccg taccggtttt ttccctcatg attacgcttc ggcatatgcg
      601 gcattgaagc agtcggaata tgtggacagt attgtcaaat tctcgcattt ctcctgtgcg
      661 gacgaacccg aaagcggtat aacggaaata cagatggaag cattcgattt gggtactgaa
      721 gggctggaag gcgaagaaag ccttgccaac tccgccgcta ttttgaatgt tcccgaagca
      781 cgcagggact gggggcgcgc gggcttggcg ttgtacggca tttccccgtt cggaggcagc
      841 gatgacaggc tgaaacccgt gatgaggctt tccacccgta ttttcggcga acgcgtttta
      901 cagccgcact cccctatcgg ttatggcgca acattttata ccagcaaatc tacgcgggtc
      961 ggcctgattg cctgcggtta tgcggacggt tatccgcgcc gtgccccaag caattccccc
     1021 gtcgccgtcg acggtaaatt gacccgggtc atcggcaggg tctctatgga tatgatgacc
     1081 atcgagctgg acgcttcaca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg
     1141 gtcaacatca ataccgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat
     1201 atcaaacgtg caaaattcac ttatatcgag taaccgggtt caaacgaaaa tgccgtctga
     1261 agcctttcag acggcataaa tgcgttttga caaaatcagt aaaagacatt gccaaataga
     1321 aaatactgat aatgcccaca atctgaaacc ccatccttcc cgcaaaaacg gaaaatcaaa
     1381 atcagaaacc taaaatttcg tcattcccgc gcaggcggga atccggtttt ttgagtttcc
     1441 gtcatttctg ataaattctc gtcgtttttc atttctagat tcccgcctgc gcgggaatga
     1501 caaaattaaa gtttcagaat ttatttgaac aacaaactag attcccactt tcgcggggat
     1561 gacggcggat aggttttgtt gtttcggata aattcctgtt gttttttggt ttccagattc
     1621 ccacctttaa aagaatgacg taagaaagca gaaataagga cacagggatt ttttaaattt
     1681 gcaatattct tttctaaaga cattttaccc gatgcctata aacaaaaatg ccgtctgaag
     1741 cctttcagac ggcatttccc catcaaaacc gcaatcagtt tttcatcgat tgaaccggtg
     1801 cgggaatcct gccgccccgg ttgacgaaca cttcgcatga gccttctttt accggcatta
     1861 caggcgcgta gcccaacagg ccgccgaact cgacgctgtc gccgacggtt ttaccggtta
     1921 ccggaataat gcgcacggca gtggttttgc tgttgatcat gccgatggcg gcttcgtcgg
     1981 caatgatgcc ggaaatggtg tgcgcgggtg tgtcgccggg aacggcaatc atatccaagc
     2041 cgaccgaaca aacggcggtc atggcttcga gtttgtccag cgtcagcacg cctgcttcgg
     2101 cggcggcaat cataccttcg tcttcggaaa cggggataaa cgcgccactc aaacccccga
     2161 ccgcgctgga agccatcatg ccgccttttt tcacggcatc gttcagcaat gccaaagctg
     2221 ctgttgtgcc gtgcgtaccg cagacgctca agcccatttc ttcaagaatg cgtgccactg
     2281 agtcgccgac ggcgggggtc ggcgccagcg acaagtcgag aataccaaac gggatattca
     2341 gcatttttga ggcttcgcgg ccgatgagtt cgcccacgcg ggtaattttg aaagcagttt
     2401 tcttcactac ttccgcaact tcggtcaatg tcgttgcatc tgaattttcc aacgcggctt
     2461 ttacgacacc tgggccggat acgccgacat tgataacggc atccgcttcg cccgaaccat
     2521 gaaacgcgcc cgccataaac gggttgtctt ccaccgcgtt gcagaacacg acaattttag
     2581 cgcagccgaa accttcgggc gtgatttccg ccgtgcgttt gacggtttcg cccgccagct
     2641 tgaccgcatc catattgata ccggcacgcg tactgccgat attgatggag ctgcacacaa
     2701 tatcggtagt cttcatcgct tcgggaatgg agcggattaa cacctcatcc gaaggcgaca
     2761 tccctttttg caccaacgcg gaaaaaccgc cgataaaaga cacaccgatg gctttggcag
     2821 ctttatccaa agtttgcgcc acgctgacgt aagaatcagc atgggtggcc gccgcgattt
     2881 gggcaatcgg cgtaacggaa atgcgctgat tcacaatcgg tacgccgtat ttggcagaca
     2941 gatattttgc cgtagtgacc aagtctttgc cgactgtggt aattttattg taaatatttt
     3001 ggttcaacac attgatatcg ctgctgatgc agtcgtgcaa atcaatgccg atggtaatgg
     3061 tgcggacatc aaaattctgg tcggcaacca ttttgacggt ttctaaaatt tcgccggatt
     3121 ggatactcat cacattcctc caactcaaat gcggtgcatc gcttggaaga tttcttcgtt
     3181 ttgcatacgg atatcaagcg cgagtttttt gctctcttcc gcaaacaaat ccaaaacctc
     3241 ttgacgcgat ttgctgcatt ttgaagtgtc caccaagata atcatagtaa aaaaatcgtc
     3301 catcagctgt tggctgatgt tgagaatatt gatttggttt tccgccaaaa ttttggaaac
     3361 atcgtacacg atgccgacgc ggtctttacc gatgacggtg atgactgaat tgttcacagg
     3421 cttactcctt gcagatatcc gttaaagtcc gaaattatac caccgttgga ttttgaagaa
     3481 atattgtcaa caatatatac atacaaaatg ccgtctgaaa ctatttcaga cagcatcaag
     3541 attcagggtt cgattaaata accatcctta tcccactggg ttttcctgac caacttgtca
     3601 tcctgataaa cagcttcgct ctttttagaa ccatcttcat accactccaa aaccaccccg
     3661 ttgcgttgat ggtggcggat agacagttcc gagagtaatc ggccgctttc atcccaagtc
     3721 agaattttgg caggctcatc gttgaccata accatttccg tcttgatact gccgtcggca
     3781 taccattgct tccatacgcc gtttgcctta ttttgcttaa actggatttc gctttccttg
     3841 ccgccgttac ggtaatagcg gtatcccgta ccctcactca agccattttt ataaggcata
     3901 acggcagatt ttttaccgtt cggataccag ttgacccact ccccgtccgg cttacccttg
     3961 ctgaagcccc ccgccatttt tttctgacca ttaaaatgcc acaaaatcaa cataccgttt
     4021 tgcagggtag gcacaaaaga tttgatttgc gttgaagcaa cgatataagg ttcagaatat
     4081 ttcttcatcg acggataata aaaatcctgc gcgtgcgcaa tacccgccac cacactatat
     4141 tgcctgatat aagcggcaga agacatcgtc gccgtcagct ttccgttctg attaaaataa
     4201 acagaatagg tctgcgccgg caaagcggcc gaaaaaccca acaggacagt tgaaaataca
     4261 atccgagata attttttcat tgcaatagcg atataaaaac aaggctgtgt tttagtaatc
     4321 tgttgatttc aattatttgc aagggaaaag acaattattt tccggttagg aataaaccta
     4381 ttctattgaa tatattgaag ccaagtacgc ctatcaacac tatattaaaa cactgccaaa
     4441 aacaattaac ttataaacaa tatggtaagg atttctctgc caagcatcaa acccgagaca
     4501 acgtatcgta aaaatgccgt ctgaaaacaa atcgtcttca gacggcattt ccccttcaac
     4561 tcactcttca cccaataact gctcgcgcgt caagaggaaa acaaaaccgt cgcccccgct
     4621 ggtttccaac caagtaaaag gcaactccgg atacgctgct tccaatacat ccctgttatg
     4681 cccgatttcc accagcaata cacctttggg attcagaaac tttgccgcat tcagaagaat
     4741 ctgcctggtg gcatccaacc cgtccgcccc gctgcccaat gccaattccg gttcgtgcaa
     4801 atactcttca ggcaataact caaccgattc cgcatccaca taaggaggat tggaaacaat
     4861 caaatcataa gtgccttcca atccttcaaa caaatccgta tgaataagcc ggatgcgttc
     4921 ttccaaacca taatcttcga cattaatccc tgccacttcc aaagcatcca agctcacatc
     4981 aaccgcatca atttgggcat caggataatg atgcgccatc tgaatggcaa ggcaaccgct
     5041 tccggtgcaa agatccaaag cattatgcac caactcatcg tattctatcc aaggacgaag
     5101 tccgtcaccc aacaattcat aaataaaaga acgaggtatg attacgcgct catccacata
     5161 gaaatcaaac tctccctgcc atgcctggtg tgtcaaataa gcggctggaa tgtgttcgac
     5221 agcacgacgc tcaataaccg ccagcacttc ctctttttca gcttccaaga gttttgcatc
     5281 aagatatggg gcaagcatat ccaaaggcaa attcaaagta tgcagaatca aataagctgc
     5341 ttcatcatgc gcattatctg ttccatgacc aaaaaagagc cctgcctcat taaaacggct
     5401 gactgcaaaa cgtaaaatat cgcggatagt cgtcaattct tgtgctgcct gattaaacat
     5461 aatatgaacc attctgcgta tagatacttt taattataac agaaacaaca agcaaacctt
     5521 ttcatatcgc caaataacca cccaatctac ccatacaact acataaatgc ccgcgcgaaa
     5581 accatcgccc gaacggaaac gacaatggcc gacggtatgg gcaatctgat tggctgggaa
     5641 aaaacggggc ttgttgtcgg taagcagtgg ataaccgcaa aagacgacaa ggtgtccgat
     5701 gtctgcaatg ccaacggcga gatgggcgta atcgggcttt acgagccttt ctcacacggc
     5761 gcattgacga tacccggtca tccgaactgc cgatgcgagg ttgtttccgt atcgggtggc
     5821 gaattggggg aatttgccga aaaaaaggag cttcgtaaag cggctatgca gtatgcgcgg
     5881 gataacttta tcggcaaaag ctatgtcaat aaaaacagcg ggcatgaact gaaggtaact
     5941 tggcaaggtg tgaaacacgc tgcgtcaaag gcaaatcagg cggaattatc catcatgaca
     6001 aaacttgatg acttattgcg ctacgcaaaa tatgagggtt cttattcgga taggaaaggt
     6061 catcctaata ttattgcagc acataagtat cgtgccgttg ccaaggttgg gaatgagtct
     6121 ttaaatatcg gtgtgattgt aagggaattt ccagacgacc ataaacatta cgaccatttc
     6181 atcttgaagg atgaataaag cccttttgca gtgtcgttct ggagcggata gcgttaaggc
     6241 aagtacactt ccagccttga aaaagggctt taaattcagc atgccattta tacaggcagg
     6301 agtaaaccca tgacaaagtt atacgcagaa atcgccaaga tggagacgca ggacgacgac
     6361 acggtcaagg tttggggtta cgcttcaagc gaggaaatcg attcggacgg cgaagtcatc
     6421 gcggcggcag ctatgaaggc ggcgattccc gattatatga agtttggcgc ggggcgcgag
     6481 atgcacggct caaacgctgc gggaacggca attgaaatca acgttgaaga cgacggcata
     6541 acctttttcg gtgctcatat catcgacccc gttgtcgtgt cgaaagtcaa aacaggcgtt
     6601 tacaaaggct tttccatcgg cggcagcgtt accgcccgcg atgatttgaa caagtcgcaa
     6661 atcacgggct tgaagctgac ggaaatcagc cttgtcgagc gccctgccaa tcccgatgcg
     6721 gtgtctacct gctttaaggc ggacaaaggt gcggaagcgg taaacaacga tacagaacat
     6781 aatgctacat attttagcc
//
LOCUS       DAXHPG010000114         6751 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXHPG010000114 DAXHPG010000000
VERSION     DAXHPG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.114"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(110..469)
                     /gene="rplT"
                     /locus_tag="ACFM6F_001600"
     CDS             complement(110..469)
                     /gene="rplT"
                     /locus_tag="ACFM6F_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC6164989.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(482..679)
                     /gene="rpmI"
                     /locus_tag="ACFM6F_001601"
     CDS             complement(482..679)
                     /gene="rpmI"
                     /locus_tag="ACFM6F_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC6164990.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(826..1347)
                     /gene="infC"
                     /locus_tag="ACFM6F_001602"
     CDS             complement(826..1347)
                     /gene="infC"
                     /locus_tag="ACFM6F_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC6164991.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="ACFM6F_001603"
     CDS             complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="ACFM6F_001603"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC6164992.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGRVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3332..3408)
                     /locus_tag="ACFM6F_001604"
     tRNA            complement(3332..3408)
                     /locus_tag="ACFM6F_001604"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3372..3374),aa:Val,seq:gac)
     gene            3587..4702
                     /gene="tgt"
                     /locus_tag="ACFM6F_001605"
     CDS             3587..4702
                     /gene="tgt"
                     /locus_tag="ACFM6F_001605"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC6164993.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAIGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQARFHENRARGTD"
     gene            4712..5794
                     /gene="hemH"
                     /locus_tag="ACFM6F_001606"
     CDS             4712..5794
                     /gene="hemH"
                     /locus_tag="ACFM6F_001606"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC6164994.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            5986..6387
                     /locus_tag="ACFM6F_001607"
     CDS             5986..6387
                     /locus_tag="ACFM6F_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6164995.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(6450..6740)
                     /locus_tag="ACFM6F_001608"
     CDS             complement(6450..6740)
                     /locus_tag="ACFM6F_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6164996.1"
                     /translation="MAKYFTETGIGVREHFDFFGEFVVSPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGGGNIGSMGLKVPR"
BASE COUNT         1720 a         1823 c         1576 g         1632 t
ORIGIN      
        1 aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa aatcaatcac atagaatttc
       61 tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa ttttttggat taagcagcca
      121 aagcagcttt ggctttttca accaattgtg caaaagcggc tttatcgaac acggccaaat
      181 cagccaatac tttgcggtcg atttcgatag aggcgcgttt cagaccattc ataaatttgc
      241 tgtaagacaa cccgttttca cgcgcacctg cattgatacg gacaatccac aattgacgga
      301 attggcgttt gcgttggcgg cggtcgcggt atgcgtattg gccggctttc attaccgcct
      361 gcttggcaac gcggtaaacg tttttacgac ggccgcgata gcctttggct aacgcgaaga
      421 ttttttggtg acgggcacga gcggtaacac cgcgttttac gcgtggcata ttctaaactc
      481 cttaagcgta gggtaacatt ttagcaacag aagccaaatc gcgatcattt accatagagg
      541 taccgcgcag ttggcgtttg tttttggtgg tctttttagt caagatgtgg cgtttgaacg
      601 catgagcgcg tttcacaccg ccgttaccca gtactttaaa gcgttttttc gcgctagact
      661 tggttttcat tttaggcatg ggaaaactcc attcgttatc ggataaggca ttagggggtt
      721 ttaaaacatc ggtttcaaac acttgaaccc acaatgccac ggttttttgc cgcaattgaa
      781 aacttactcc gaagcggcaa tcggagtaag cggagaatta tagctttatt ttttcttcgg
      841 cgcaatcatc atcaccattt ggcgaccttc cattttggga aaggactcga tttgcgccac
      901 ttcagccaaa tcttctttta cacgttccaa aagttgcgcg ccgagttgct ggtgagccat
      961 ttcacggccg cgaaaacgca atgtcacttt gactttatcg ccgtcggcaa ggaagcggtt
     1021 aatgttgcgc atcttgattt gataatcgcc ctcatccgta cccggacgga atttgatttc
     1081 cttgatttgc acctgctttt ggtttttctt ggcttcgtcg cgcttcttgg cttgttggta
     1141 tttgtattta ccgtaatcca tcagtttgca cacaggcggt ttagcagttg gggaaatctc
     1201 taccaaatcg acatcctgcc cttcggccat agccaaagct tcacgaactg aaacgacacc
     1261 aagctgttcg cctgactcac tgattaaacg cacttctttg gcggtaattt cgccgttgat
     1321 tcgtgcttcg cgttcttgag cgatgatgaa tactcctata aaaattaatg attgacgagg
     1381 gcatcagtga tttcttgctg caattgcgca atgaaatcat ccaaatccaa agaacccaaa
     1441 tcttctgctt tgcggcgtac cgccactttg ttttcctgct tctccttatc gccgacaacg
     1501 atttgataag ggaaacggta ttggctgttg tcgcggattt tgtaaccgat tttttcgtta
     1561 cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt tggcagccac ttcacggcag
     1621 taatccgctt gattttcggt gatattcata atcaccattt gaaccggcgc caaccataac
     1681 gggaatgagc ctgcatggtt ctcaatcaga atgccgataa accgctccaa agaacccaaa
     1741 atggcgcgat gcaacataac aggacgcgca cggtcgttgt tttcagttac atattcggca
     1801 ttcaaacgct ccggcaatac gaaatccagt tgtaatgtac cgcattgcca agaacgaccc
     1861 aaggcatctc tgacatgata ttcgatttta ggcccgtaaa acgcaccctc gcccggcaat
     1921 tcgccccatt ccacgccgca ggcagtcaat gcctcgcgca aaccctgctc tgccttatcc
     1981 cacacgtcat ctgaacctgc gcgtttttca gggcgaagag aaagcttgac ggatacatca
     2041 tggaaaccga actgtttgta gatgcgaacc aacaattcat tgaacgcacg ggcctcgctg
     2101 acgatttggt cttcggtaca gaaaatatgc gcatcatcct gcacaaatcc acggacccgc
     2161 atcagaccat gcaatgcacc gcttggttca ttgcggtggc aagaaccgaa ctcggccaaa
     2221 cgcatcggca aatcgcgata tgagcgcaaa ccgttattaa aaatttgcac atgacccgga
     2281 cagttcatcg gtttcaccgc gtattcgcgt ttttctgaac tggtcacgaa catattgtct
     2341 ttgtagttgt cccaatggcc ggatttttcc caaaaggttt tatccataat ttgaggcgtt
     2401 ttgacctctt tataaccggc ggcgttcagc tctttacgca tatgctgttc aatcacttgc
     2461 cacaaagccc agcctttagg atgccaaaac accatgcccg gcgcttcgtc ttgcagatgg
     2521 aacagatcca attgcttgcc aagtttgcgg tggtcgcgct tttcggcttc ttcgatacgt
     2581 tgaatatagg cttttaattc gtcttttgtc gcccaagccg taccgtatat gcgttgcagc
     2641 atttcattat tgctgtcgcc gcgccagtat gcgcccgcca acttggtcag tttgaagttt
     2701 ttcaggaaac gggtattcgg aacgtgcggg ccgcggcaca tatcgacata ttcctgatga
     2761 tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa tcaggcgcag tttgtattct
     2821 tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg gagtcatgat tttgaccaca
     2881 tcatagtctt gggcaatcaa ttctttcata cgcgcttcaa tggcggcaac atcttccggt
     2941 gtaaacggtt tttccgtggc aatgtcgtaa taaaagccct cttcaatgac ggggccgata
     3001 accatttttg cattaggata gagttgcttg acggcatgcc cgacaagatg ggcgcaggaa
     3061 tggcggatga tttcgatgcc ttcctgatct ttcggagtaa tgatttgaac agcagaatct
     3121 tcaacaatcg ggtcgcacgc atcgaccaat ttgccgttta ccctgcctgc caccgccgcc
     3181 ttcgccaaac cggcaccgat agacgcagca atttgagcca cggtaacggg ggattcgtat
     3241 tgtcggactg agccgtccgg caaggtaatg ttcaacatca aaaagctcca aaacattaaa
     3301 aaataacggc ataaatgccg ttatgggaat ttggtaggca cgattggatt cgaaccaacg
     3361 acccccacca tgtcaaggtg gtgctctaac caactgagct acgtgccttc aaagaatttt
     3421 tgcattttat cgggtcgttt taatttttgc aagaggtatg tgcgtttctt tatgcaacat
     3481 tgcggcaaga tttggggatg tcgtctgaaa aaccgtggga aatcatttat aatgccgtct
     3541 tttaaaacat atttcgcccc ctgtcgccca actgacattc aatcttatgc ttaaatttac
     3601 cttacacaaa aaagacggat acgcccgacg cggcacgctg gagttgaacc acggcaaaat
     3661 cgaaacgccg gtatttatgc ctgtcggtac ttacggttcg gttaaggcga tgaacccgca
     3721 aaacctgcac gacatcaagg cgcaaattat tttgggcaac acttatcatt tgtggctgcg
     3781 tccgggcttg gaggtcgtcg aacagtttgg agggcttcac ggctttatcg gctgggacaa
     3841 accgattttg accgactcgg gcggttttca ggttttttct ttgtcggata tgcgcaagct
     3901 gacggaagaa ggctgtacgt ttaaaagccc gattaacggc gacaagctgt ttttatcgcc
     3961 tgaaatttct atgaaaatcc aaacggtttt gaactcggac atcgcgatgc agttggacga
     4021 atgcacgccg ggcgaggcga cgcgcgaaca ggcgcgaaaa tcgttgcaaa tgagcctgcg
     4081 ctgggcggaa cggagcaaaa aagcctttga agatttgaaa aacccgaacg cactgttcgg
     4141 catcgtgcaa ggcgcgatgt atgaagattt gcgcgaagag tcgctgaaag gcttggaaga
     4201 gctcgatttc cccggcttgg ccatcggcgg cttgtccgtt ggcgaaccta agcccgaaat
     4261 gtaccgcatg ttgcgcgccg tcggccctat cctgccggaa cacaaacctc attatttaat
     4321 gggcgtcggt acgcctgaag acttagtgta cggcgtggca cacggcgtgg atatgttcga
     4381 ctgcgtcatg cctactcgca atgcgcgtaa cggctggctg tttacccgtt ttggcgattt
     4441 gaagatcaaa aacgccaaac acaagctcga caagcgtccg atagacgaaa gctgcacctg
     4501 ctacgcctgc caaaacttca gccgcgccta cctgcaccat ctgcaccgcg ccggcgaaat
     4561 cttgggcgca cagctcaaca ccatccacaa cctgcatttc taccaagtca ttatggcgga
     4621 aatgcgcgat gccatagaac aaggcaaatt tgccgactgg caggcgcgat tccacgaaaa
     4681 ccgtgctcgc ggcacagact gactcccggc aatgccgtct gaagccgctt cagacggcat
     4741 tttgccgctt taccctaccc gcactatttt tcagaaaccg atcatgcttc cattccttcc
     4801 agaaccctcc ctttcctata cgcaacaaaa ccgtaccgcc gttttgctgt tgaacctcgg
     4861 cacgcccgac gcgccgaccg cgcaggcggt acgaccttat ctgaagtcct ttctgaccga
     4921 ccggcgcgtc gtcgaactgc ccaaatggct gtggtatccc attctgcatg gtctggtact
     4981 gacgttgcgc cccaaaaaaa gcgcgcacgc ctatgaaaaa atctggttta aggaaggttc
     5041 gcccctagaa gtttacacag cacgccaagc tgccgcgctt gccgaacgta tgcccgacct
     5101 catcgtccgc cacgccatga cctacggcaa tccttcggtg gcagacgtat tgtcggaact
     5161 gaaggcgcaa ggcgcgggca gactgctggt catcccgatg tacccgcaat atgccgcttc
     5221 cagcagcgga gcggcagtag ataaggtgtg cgaacaatta ctgctgcaac ggaaccaaat
     5281 gagcgtccgc accgtttcgc gcttttatga cgataccggc tacattgatg cgatgaaaaa
     5341 ccacatcctc cgatattggg cggaacacgg acgtgggaaa aaactgatgt tgagttttca
     5401 cggcgtaccg cagaagcact acgacctcgg cgacccctat cccgacgagt gccgccacac
     5461 cgccaaactg cttgccgaag cactggaact gaccgaagac caatatgtcg tatcgttcca
     5521 aagccaattc ggcagggcaa aatgggttac accgagcacg caggatttgt tcggcaaact
     5581 gcccaaacag ggggtaaccg agctggacgt attctgcccg ggctttttgg cagactgttt
     5641 ggaaaccatg gaagaaatcg ccctgatggg gcgggaacag ttttacgaag caggcggcaa
     5701 aagctaccgc tacatcccct gcctcaacga caaccccgac tggatagacg cactcgtcgc
     5761 acttgccgaa gaaaaccttg gcggctggcg ttgaaactgt ttgacccatg tctaaaatgc
     5821 cgtctgaacg ccgtgttcag acggcatttt cccgctactt tagcgtatga tggcatctat
     5881 gccggacaac aacatcaagt gcaaccttac taccgattcg gcaaaaaaat ctttcacccg
     5941 ctacgcaaaa tcaacaacaa ggtttcaaac attaaggaaa caccaatgaa cacaacccga
     6001 ctgccgaccg ccctcgtctt gggctgcctc tgcgccgccg cttctgccgc cgacaacagc
     6061 atcatgacaa aagggcaaaa agtgtacgaa tccaactgcg tcgcctgcca cggcaaaaag
     6121 ggcgaaggcc gcggaaccat gtttccgccg ctctaccgct ccgacttcat catgaaaaaa
     6181 ccgcaggtgc tgctgcacag catggtcaaa ggcatcaacg gtacaatcaa agtcaacggc
     6241 aaaacctaca acggattcat gcccgcaacc gccatcagcg atgcggacat tgccgccgtc
     6301 gccacttata tcatgaacgc ctttgacaac ggcggcggaa gcgttaccga aaaagacgta
     6361 aaacaggcaa aaggcaaaaa aaactaaaca gacaaaatgc cgtctgaaac cggcaatccg
     6421 gcttcagacg gcattcaaat caaacctttc taacgcggta ctttcagccc catagacccg
     6481 atattgccgc caccgtcaac ctccttcgcg gtcagcataa agccgccgat gtcaatacgg
     6541 ccgcactcga ccggctcctg actgtcggaa cgcttatcga aaagctcggc aaggctcaaa
     6601 ccctcttcgc ccgcttccag cctcaaaccg taagcaagtg ccaaatcacc cgaacgtgct
     6661 gccggcgaaa cgacaaactc gccgaaaaaa tcaaaatgct cacggacacc gatacccgtc
     6721 tcggtaaagt atttcgccat tttatagtga a
//
LOCUS       DAXHPG010000115         6716 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXHPG010000115 DAXHPG010000000
VERSION     DAXHPG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.115"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            18..1409
                     /locus_tag="ACFM6F_001609"
     CDS             18..1409
                     /locus_tag="ACFM6F_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6164997.1"
                     /translation="MQVFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            1488..2744
                     /locus_tag="ACFM6F_001610"
     CDS             1488..2744
                     /locus_tag="ACFM6F_001610"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC6164998.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAKGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            2925..3734
                     /gene="zupT"
                     /locus_tag="ACFM6F_001611"
     CDS             2925..3734
                     /gene="zupT"
                     /locus_tag="ACFM6F_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFC6164999.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            3831..6668
                     /locus_tag="ACFM6F_001612"
     CDS             3831..6668
                     /locus_tag="ACFM6F_001612"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC6165000.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     NLDREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFAMPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQVEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVATL
                     PESEDAPVAVCNGARLMLKVEIDKATETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
BASE COUNT         1585 a         1818 c         1801 g         1512 t
ORIGIN      
        1 agacaaagga gaaagccatg caagtgtttt tagacaatct caaagccttt ttcgaaaccg
       61 tcagcggctg ggtctgggga tctgttatgt tgatgctgct ggtcggcacc ggcattgtgc
      121 tgaccgttat gctgaaaggc ttgcagttca ctatgttggg ctatgcgctg aaacaggcat
      181 ttgtgccgtc aaagaagtat gaaggcggcg aaggccacga aggcgatatt tcccatttcg
      241 cggcgttgat gaccgcgctg tccgccacca tcggtacggg taacatcgcc ggcgtggcga
      301 ctgcggtggt aaccggcggc ccgggcgcgg tattttggat gtggatgacc gccattttcg
      361 gcatggccac caaatacggc gaaggcgtgt tggcggtgaa ataccgcgtc aacaattcca
      421 aaggcgaaat gtccggcggc ccgatgtatt acatcgaaaa aggcttgggc aaaaactgga
      481 aatggatggc cgtcgcgttt gcgctgttcg gcacattcgc ttccttcggt atcggcagct
      541 cggtgcagtc caactcggtt gcacaggcgg tgcaaaccag cttcggtatc gaacctgcct
      601 ataccggcat tacgttgacc gttctgactg ccgttgtcgt tttaggtggt attaaaggca
      661 tcgccaaagc cgcttctttc atcgtgcctg ctatggcggt gttttatgtg ttgggcggtc
      721 tttccattat cgcgattaat tccgatgcac tgatgcctgc cgtcaagctg attttctccg
      781 atgcgttcag cgcgcaggca gtggcgggcg gcgctatcgg tacggtcatc cgctacggcg
      841 tggcgcgcgg cgtgttctcc aatgaggcgg gtatgggttc tgcgcctatt gccgccgcag
      901 ccgcgaaaac cgaccatccc gtccgtcagg ccttggtttc tatgaccggt acgtttttgg
      961 ataccatcgt cgtttgctcg attaccggca tcgtgttggt aatgggactg ctcggcgcgg
     1021 gcggcgagtt tgtcaaacct gaagtgagcg gcgcggcact gacaaccgtc acattccaaa
     1081 aaatgctgcc cggcatcggc ggctggattg ttaccatcgg cctgattttc tttgcctact
     1141 caaccatttt gggctggtgc tattacggcg aaaaatgcgc ggtgtatgtt ttcggcgaga
     1201 aatttgccgg tttgtaccgc gtgggttatg tttcttccgt tatgctgggt accgtgttga
     1261 gccttgattt ggtgtggctg gcttcggaca cattcaacgg cttgatggca ttgcccaacc
     1321 tgattgcact tttattgatg gcgaaagtca tcgtcaatga aacgcgcgac ttcaaacaaa
     1381 aaatcacaaa cggcgaattg ccgcattaat atccgaggcc gtctgaatcc gaatattcag
     1441 acggcacggc atattaagtc tgttttgaaa aagtcggagg gagaaacatg aaagtgcttg
     1501 ttttaggtgc gggtgttgcc ggcgtgtctt ccgcgtggta tctggcagag gccggacatg
     1561 aagtaacggt catcgaccgc gccaagggtg tggcgatgga aaccagtttt gccaatgcag
     1621 gccagctttc ttacggctat accacgcctt gggctgcacc cggtattccg accaaagcac
     1681 tgaaatggct gtttaaaagc catccgcctt tgctgtttcg ccccgacggc agcctgtatc
     1741 aaatcgaatg gctgtggcgg atgctgcaaa actgcacggc agcgcactat caaaccaata
     1801 aagagcgcat ggtcaggatt tccgaataca gccgtgaaat gttccgccgt tttgaagcgc
     1861 aaaccggcat gaattttgaa gggcgcaaaa aagggacgtt gcagattttc cgtcaggcta
     1921 aagaagttga agctgcagaa caagacattg ccgttttgga acgctacggc gtgccgtacc
     1981 gccgtctgaa gcccgaagaa tgcgcagaat tcgagcctgc gctggcacgc gttaccgcca
     2041 aaattgccgg cggcctgcac ctgcctgcgg atgcaaccgg cgactgccgc ctcttcactg
     2101 aaaacctata caaattgtgt caggaaaagg gcgtacggtt tcatttcaac caaaccatca
     2161 gccgcatcga ccacaacggg ctgcgcatca aaaccgttga aaccgaaaca gggcggtttg
     2221 aagcagatgc cgttgtctgc gcgctcggtt gtttcagcag gacggttttg gcgcagttgg
     2281 atctcaatct gcccatttat cccgtcaaag gctattcctt gaccctgccg gtcacaaatt
     2341 ccgacggcgc accggtgtcc accgttttag atgaaagcta caaagtcgcc atcacgcgct
     2401 ttgacaacag aatccgcgtc ggcggtatgg cggaattgtc gggctacgaa atcaaactgc
     2461 ccgaaaaacg ccgcgaaacc ttggctttgg tcgtcaacga cttgttcccg gaaggcggcg
     2521 atttgagcca agcatcgttc tggagcggcc tcaggccgat gacgcccgac agcacgccgt
     2581 taatcggtcg cacccgcttt gacaacctgt ttctgaatac cggccacggt actttaggct
     2641 ggaccatgtc gctgggttcg gcaaaattaa ccgccgatat cgtgagcggc aaagacaccg
     2701 aaatccgcag cgacgatttg agcctgtcgc gttatcaggc ttaagctgga aagcgataag
     2761 gtttagcggc atttcaaatt taaaggccgt ctgaaaatca tttttcagac ggccttttgt
     2821 ttcaaaatcc gtgtatccaa tcaataaaat aaataataac tattatcata tacattaaaa
     2881 agtgttagtc tgaacctgtc ccagctttta gcgcaggttt tctcatgccc gatttttcga
     2941 tgtccaattt ggccgttgcc ttttccatca cattggctgc cggtttgttt accgtattag
     3001 gcagtggctt ggtgatgttt tccaaaacgc ccaatccgcg tgtgttgtcg tttggtttgg
     3061 cgtttgccgg cggtgcgatg gtatatgttt ccctgacgga gattttcagt aagtccagcg
     3121 aggcgttcgc tgaaatttat gataaagacc acgcgtttgc ggcggcgacc atggcatttt
     3181 tggccgggat gggcggcatt gcgctgattg accgtctggt gccgaacccg catgaaactt
     3241 tagacgcgca agacccgtcg tttcaagaaa gcaaacgccg ccatatcgcg cgagtcggca
     3301 tgatggcggc gtttgcgatt actgcgcaca atttccccga aggcttggcg acgttttttg
     3361 ccacattgga aaatccagca gtcgggatgc ctttggcctt ggcgattgcc atccataata
     3421 ttccggaggg catttccatc gccgcgccgg tttattttgc cacccgcagc cgtaagaaaa
     3481 cggtgtgggc gtgtctgcta tccggcttgg ccgagccgtt gggggcggct ttgggctatt
     3541 tggttttgca gccgtttttg tcgcctgccg tgtttggttc ggtattcggc gtgatagccg
     3601 gtgtgatggt gtttttggcg ttggacgagc tgctgccggc tgccaaacgc tattcagacg
     3661 gccatgaaac cgtttacggc ctgacaatgg gcatggcggt gattgccgtc agcctggtat
     3721 tgttccattt ttaaaacctg tatgccaaag gccgtctgaa atgattcaga cggccttttt
     3781 gcgttataat ccgcacatca aaaataacct taatttcaca aagttccatc atgttagaca
     3841 aatactcccc cgccgaaatc gaatccaagc actatcaaaa ctgggaaagc caaggctatt
     3901 tccaacctga tatggatttg accaaaccgt ctttttccat ccaactgccg ccgcccaatg
     3961 taaccggcac gctgcacatg ggtcatgcct tcaaccaaac cattatggac ggcctgaccc
     4021 gctactaccg tatgaaaggc tgcaacaccg cctggattcc cggcaccgac cacgcgggca
     4081 tcgccacgca aatcgtggtc gagcgtcagc ttgccgcgca aaacgtgtcc cgtcataact
     4141 tggatcgtga aaaattcttg gaaaaagtgt gggaatggaa agaagtttcc ggcggcacga
     4201 ttacgcagca gatgcgccgt gtgggctgtt ctgccgactg gacgcgcgag tatttcacaa
     4261 tggacgatgt acgcgccgaa accgtaaccg aagtgttcgt acgcctgtat gaacagggct
     4321 tgatttatcg cggcaaacgc ttggtgaact gggatccggt attgggtacc gcagtgtctg
     4381 atttggaagt ggaaagcgtg gaagaacaag gctctatgtg gcacatccgc tatccgctgg
     4441 cggacaatcc tgccgaagcc gtcatcgtcg ccaccacccg tcccgaaacg ctgttgggcg
     4501 acgttgccgt tgccgtcaat cccgaagacg aacgttatac ccacttaatc ggcaaagaat
     4561 taatcctgcc gctgaccggc cgcaccattc ccgtgattgc cgacgaatac gttgaaaaag
     4621 acttcggcac cggctgcgtg aaaatcacgc ctgcgcacga cttcaacgac tacgaagtcg
     4681 gcaaacgcca cgatacgcgc ttggttaatg tgttcgactt ggaagccaaa gtattggcaa
     4741 acgccgaagt gttcaacttc aaaggcgaag cgcaaccaag ctttgccatg cctgaaaaat
     4801 acgcaggctt ggaccgcttt gccgcgcgca aacaaatggt tgccgatttg caggaacaag
     4861 gtttcttggt cgaaatcaaa gcgcacacgc tgatgacgcc gaaaggcgac cgaacaggct
     4921 cagtgattga gccgatgctg accagccaat ggtttgtcgc catgtccgct acgccaaacg
     4981 gcggcgagcc tgacagcgaa ttcaaaggct tgagcctcgc cgacaaagcc aaaaaagccg
     5041 tcgacagcgg cgcggtacgc tttatccccg aaaactgggt caacacctac aaccaatgga
     5101 tgaacaacat ccaagactgg tgcatctcgc gccaactgtg gtgggggcat caaatccccg
     5161 cgtggtacga cgaagcaggc aatgtttacg tcgcccgcaa tcaggtggaa gccgaaaaac
     5221 aagccggcaa aacaggcttg acccgcgaag aagacgtatt ggacacttgg ttctcctccg
     5281 cgctcgtgcc gttctccacg ctcggctggc cgtctgaaac cgacgaactc aaagccttcc
     5341 tgccgtccaa cgtcttggtt accggctacg aaatcatctt cttctgggtg gcgcgtatga
     5401 ttatgatgac cacccacttc accggcaaag taccgtttaa agacgtgtat atccacggca
     5461 tcgtgcgcga ccacgaaggc aaaaaaatgt ccaaatccga aggcaacgtc attgaccctg
     5521 tggatttgat cgacggcatc ggcttggaaa aactgctggt aaaacgcacc accggcctgc
     5581 gcaaacccga aaccgcgccg aaagtggaag aagccacgaa aaaactcttc cccgaaggca
     5641 ttccgagtat gggcgcggac gctttgcgct tcactatggc gagctacgcc agcttggggc
     5701 gttcggtcaa cttcgacttc aaacgcgccg aaggctaccg caacttctgc aacaaaatct
     5761 ggaacgccac caacttcgtc ttgatgaaca ccgaaaacca agactgcggt tacggcgcaa
     5821 ccgctgccga accgcgcggc tactccttcc ccgatatgtg gattgtaggc cgtctgaatc
     5881 agaccatcga gcaagtaacg caagcctacg aaacctaccg ctttgatttg gcggcggaaa
     5941 ccctgtacag cttcgtgtgg aacgactatt gcgactggta tctggaactt gccaaagtgc
     6001 agctccaaac cggctgcgcc agccgccaac gcgccacacg ccatacctta ttgcgcgtac
     6061 tcgaagccgc cctgcgcctg ctgcacccga ttatcccgtt catcaccgaa gaactgtggc
     6121 aaaccgtcgc ccctatgtgc gacgcgaaaa ccgccgacag catcatgctc gcccgcttcc
     6181 ccgaagccga cggcggcgat attgttcaga cggcattcgg gcaaatgacc gtgttacagg
     6241 atttgattgg cgcagtccgc aacctgcgcg gcgaaatggg catccagccc aacgtgaaag
     6301 ccccgctgtt tgtcgaaagc gcagacgact tggcggacta cctcaaatac ctgccgatga
     6361 tgacccgact gaccgaagca cagcaagtcg ccaccctgcc cgaaagcgaa gacgcgcccg
     6421 tcgccgtctg caacggcgcg cgcctgatgc tgaaagtcga aatcgacaaa gccaccgaaa
     6481 ccgcccgttt gagcaaagaa gccgaaaagc tgcaaaaagc cttggacaaa ctcaacgcca
     6541 aactctccaa acccggctac accgaaaaag ccccggcgca tttggtggaa aaagacaaag
     6601 ccgatttggc ggaattggaa gacaaaatgg caaaagtgca aactcagttg tcgaagttga
     6661 aagactgatt tgattgaatc aacaaggccg tctgaaaaat gtttttcaga cggcct
//
LOCUS       DAXHPG010000116         6644 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXHPG010000116 DAXHPG010000000
VERSION     DAXHPG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.116"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            716..2164
                     /gene="gnd"
                     /locus_tag="ACFM6F_001613"
     CDS             716..2164
                     /gene="gnd"
                     /locus_tag="ACFM6F_001613"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC6165001.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2227..3498
                     /gene="waaA"
                     /locus_tag="ACFM6F_001614"
     CDS             2227..3498
                     /gene="waaA"
                     /locus_tag="ACFM6F_001614"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC6165002.1"
                     /translation="MFQWLYDALWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3539..3988
                     /locus_tag="ACFM6F_001615"
     CDS             3539..3988
                     /locus_tag="ACFM6F_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC6165003.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4021..4995
                     /locus_tag="ACFM6F_001616"
     CDS             4021..4995
                     /locus_tag="ACFM6F_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC6165004.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFVFLKYGLA
                     FVLGFIGVKMLIMHWVHIPISVSLSVVFGALGASVLTSLVYTKKQSDK"
     gene            5064..5139
                     /locus_tag="ACFM6F_001617"
     tRNA            5064..5139
                     /locus_tag="ACFM6F_001617"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5097..5099,aa:Lys,seq:ttt)
     gene            5358..6611
                     /gene="murA"
                     /locus_tag="ACFM6F_001618"
     CDS             5358..6611
                     /gene="murA"
                     /locus_tag="ACFM6F_001618"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC6165005.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMVVEGA
                     GELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
BASE COUNT         1525 a         1607 c         1919 g         1593 t
ORIGIN      
        1 agcggtttct gttttttctg ataaattcct aaaacttaaa atttcatcat tcccactttc
       61 gtgggaatga cgggatgtag gttcgtggga atgacgcggt acaggtttcc gttacgtatc
      121 cccatccgaa agtttattta tacacaagca atccaaagtt atctgactga aatattgtga
      181 gatttacccg caaatctcat tttttccacc acttatacac aaattcggat gcgtactctg
      241 ccgatatgca gaaataagac attcgtgccg acacctgacc atttccacaa gttccaaaca
      301 cgcttccggc ttaaatctcc acagtattcc cgttcttaaa ggcgccagca gccattcggt
      361 aaagaaaagt aggatgccat ctgcacaaca agcaaactat ccacaaaact tattccccat
      421 aaaaaacaat ccacaggtat gcacaaaagg gaatccgcct taccgacacc cttttaaaac
      481 ggaaatccca atgattttta aaataaaaaa aagttaccca cagaaaattt ctcctatcaa
      541 tataatcatc gtttttttat ttaattttta tattttattt ataagccttc agggaaacgc
      601 acggtaaagc gtaaaggcat cttttcaaaa aattaaaaag ttaaggattg ttaaacaagt
      661 gtaaatcagg tagagttgcg gtcgataaat taaatctata acggagcttc ctgcaatgaa
      721 aggcgatatt ggcgtaatcg gtttggcggt tatggggcaa aacctgattt tgaatatgaa
      781 cgattgcgga tttaaggttg tcgcctacaa ccgaacaatc ggtaaggtgg acgaattttt
      841 aaacggcgcg gctaagggaa cggatattgt cggcgcgtat tccctgcaag acttggtcga
      901 taagctggca aaaccgagaa aaatcatgat gatggttcgc gcaggttcgg tggttgacga
      961 ctttatcgaa caactgctgc ccctgttgga cgaggaagac atcattatcg acggcggcaa
     1021 tgccaattac cccgacacga cacggcggac acattacctt gccgaaaaag ggattttgtt
     1081 tgtcggggcg ggcgtatccg gcggcgaaga aggcgcgcgg cacggcccgt ccattatgcc
     1141 gggcggggat aagcgcgctt gggaagcggt caaaccgatt tttcaggcga ttgccgccaa
     1201 aacgccgcag ggcgaaccgt gttgcgattg ggtcggcagg gatggtgcgg gacatttcgt
     1261 caaaatggtg cataacggca tcgaatacgg cgatatgcag ttgatttgcg aagcgtacca
     1321 gttcatgaaa gacggtttgg gactgtccta cgacgaaatg caccgcgtgt ttgccgagtg
     1381 gaacaaaacc gagctggatt cttatctgat tgaaatcaca gcggcaattt tgggctataa
     1441 ggacgaaggc ggcgaaccgc tggtcgagaa aatcctcgat acggcagggc aaaaaggcac
     1501 gggcaaatgg acgggcatca atgctttgga tttgggtatt ccgttgacgc tgatttccga
     1561 agcggtattc gcccgctgcg tttcgtcgtt caaagaacag cgcgtgcaga ccggcaagct
     1621 gttcgcccga accgttacgc ccgtcgaagg cggcaaacaa gaatgggttg aggcgttgag
     1681 acaggccctg cttgcatcca aaatcatttc ctacgcacaa ggctttatgc tgatccgcga
     1741 agcgggcgaa agctacggct gggatttgga ctacggcaac actgcgctgc tgtggcgcga
     1801 ggggtgcatc attcgcagcg catttttaag caatatccgc gatgcctatg aaaacaatcc
     1861 cgatttggtg ttcttgggtg cggatccgta tttcaaaaat attttggaaa actgcctgcc
     1921 ggcgtggcgc aaggtggttg ccaaggcggt cgaatgcggc atccctatgc cctgcatggc
     1981 ttcggcgatt acgttcttgg acggctacac gaccgaacgt ctgcctgcca acctcttgca
     2041 ggcacagcgc gactacttcg gcgcgcacac ttacgaacgc accgacaaac cgcgcggcga
     2101 gtttttccat accaactgga cgggcaaggg cggagatacc gcttcgacaa cctacgatat
     2161 ttgattccgt tgccgaaatg ccgtccgaac cgttcggacg gcatttgttt tgcaggaaat
     2221 agaaagatgt tccaatggct ttatgatgca ttgtggctgc ttgcgccgat atggatacgg
     2281 cgttatttgg acaaacgctc cggaagtgcc cctgcatatc gggcgcatcg ggacgagcgt
     2341 ttcggcaagc cgtatccgaa tcccgttacc ggcgcggttt ggatacacgc cgtttcggtc
     2401 ggagaaacgc gtgccgccca gcccttgata cgcgagttgc ggcagcgttt tcccgatgcg
     2461 ccgctgctga tgacgcagat gaccccgacg ggacgggaaa ccgcgcaagt tctgtttccc
     2521 gatgcgcaat gccgctatct tccgtatgac aaaaaaacgt gggtacggca gtttttgcgc
     2581 gaacaccgcc cgatgttcgg cattttgatg gaaaccgaaa tctggcccaa tctgatgaaa
     2641 gaatgccggc gcgcgggtgt gccgctgttt ttggcgaatg cgcggctgtc ggaaaaatcg
     2701 ctgaacggtt atctgaaagt ccgccgcctg atccgtcctg ccgccgcttc gctgacgggc
     2761 tgtctggcac agacggaggc ggatgcggcg cggttggcga aattgggcgc ggcatccgtg
     2821 caggtgtgcg gcaataccaa atacgacctg atgccgtcgg aacagatgaa aacgctggcg
     2881 gggcagtttg aaaaacgcat cggaggccgg ccggttgccg tgtgcggcag cacgcgcgtt
     2941 tatcggggcg aagacgaggc ggaaaaactg ctggcggcgt ggcaacaata tcgcggcgat
     3001 gcgctgctgg tcgtcgtgcc gcgccatccc gagcattttc agacggtatt tgaaacggca
     3061 aaacgcttcg ggtttaaggt tcagcggcgc agcgacggtt tgccggtcga acctgatacg
     3121 caggtgtgga taggcgacag tatgggcgag ctgtatgcgt attacctgtg cgccgatgtc
     3181 gcttttgtcg gcggcagtct ggtcgattcg ggctgtcaga acattatcga accgctttcc
     3241 tgcggcgttc cgacgatatt cggcttttca acctacaatt tttccgaagc ctgccggcac
     3301 gccttggcat cgggggcggc ggttcgggtt gagtcggcgg acgcgtggcg ggaagccgtt
     3361 gaaaaaacct tatcgtccga ggggggcgga atgcagatgc aggcgcacgt ggacggcttt
     3421 atcgcacaac atcgcggagc gggcgcgaga atcgccgagg cggtacggga agcggtatgc
     3481 ggacatcggg gacgatagtg atacaatccg tatccgagtt ttccggttgg agcatcgtat
     3541 gagtatttat gccgtcgcgc acatcgtcca cctgtattgc gccattgcct ttgtcggcgg
     3601 cgtgtttttt gaagtgctgg ttttgtccgt cctgcatacg ggacgggtgt cgtgcgaggc
     3661 gcggcgcgaa gtggaaaagg caatgtctta ccgcgccgtc agggtgatgc cgtttgtggt
     3721 cggactgctg ttcgccagcg gcgtcgtgat ggcggcaaac cgctatcttt ctatattggg
     3781 cgaaccgttt gccacttcct tcggtacgat gctgacgctg aaaatcctgt tggcgttcag
     3841 cgtgttggcg cacttcgcca tcgccgtcgt caaaatggcg cgttccacac tgaccgtcgg
     3901 ctggtcgaaa tacatacacg ccgtcgtctt tacccatatg ctgctgattg tctttttggc
     3961 aaaagcgatg ttttatatca gctggtaaca caattacatt tcaaatcagg agattgaacc
     4021 atgacagaat atcccggcat cgggtcgccg ttgttttacg gcgttttttt tgcggcagtg
     4081 ctggtcatga ttgccttgga tatgttttcg ctgaagaaaa acggcagcca taaagtcggc
     4141 gtcaaagaag ccttggcatg gagcggcttg tgggttgccg tatcctgcct gttcgcgggc
     4201 tggctgtatt tcgaactcgc cggcaatctc ggctacggcg cggcagtcgc aaaagaaaaa
     4261 gtattggaat tctttaccgg ctacattttg gaaaaatcgt tggcagtcga caatattttc
     4321 gtgttcctga tgatattcgg ctacttcaaa gtcgcaccac agtttcagca ccgcgtgctg
     4381 ctgtacggcg tattgggcgc attggtattg cgcactgtca tgattttcgt cggcgcggca
     4441 ctggttcagc agtttgagtg gattctgtat ctgtttggcg cgttcctgct ctatactggt
     4501 atacacatga tgaagcccga aggcgatgag aaagaggatt tggcaaacag caggctgctg
     4561 aatgccgtca agaaagtcgt tccggtcggc acggaatttc acggcgagaa attttttact
     4621 gtcgaaaacg gtaaaaaaat cgccacgccg ctgtttttgg tgctggtcat gattgaattg
     4681 agcgatgtcg tttttgccgt ggacagcatc cccgccgtct ttgccgttac caccgatccg
     4741 tttatcgtgc tgacttccaa tattttcgca attctgggct tgcgggcgat gtatttcctg
     4801 ctggcggatg tggcggaacg cttcgtattc ctgaaatacg gcttggcatt cgtgttgggt
     4861 tttatcggtg tgaaaatgct gataatgcat tgggtgcata tcccgatttc cgtttcgctg
     4921 tcggtcgtgt tcggcgcatt gggtgcatcg gtgttgactt cattggttta tacgaaaaaa
     4981 cagtccgata aataaaatgc cgtctcaacg ccgacaaaat ggttgacagc atcaggcttg
     5041 atgtatagaa tttcgagctt gtcgggtcgt tagctcagtc ggtagagcag cggactttta
     5101 atccgttggt cgagcgttcg aatcgctcac gacccaccag ataaaacaaa aaagctgagt
     5161 tttcaaaact cggctttttt tgttttccga caatccttgt tcggaaagcg cggcatttcg
     5221 tcaaattctt tggtgcggct tccgtatttt tcccgaaaat cccttataat tccgcctctg
     5281 caaccttcat cgggtgatgc tttatgcgtt gtcccgagta ttgaaatatt acaagtaccc
     5341 accaggaaag aacaaacgtg gacaaactga aaatctccgc aaatggcccg ctcaacgggg
     5401 aaataacggt ctcgggcgcg aaaaacgcgg cattgccgct gatgtgcgcg ggtttgctga
     5461 catcgggtac gttgcgcctg aaaaacgtcc ctatgctggc agatgtgaaa accacgcaaa
     5521 agctgcttca ggggatgggc gcgcgcgtcc tgactgacaa tatcagcgaa tttgaaatca
     5581 acggcggtac ggtaaacaat acctgcgccc cttacgaatt ggtacggacg atgcgggcct
     5641 cgattttggt gctggggcct actttggcac gtttcggcga ggcgcaagtc agcctgccgg
     5701 gcggctgcgc catcggttcg cgccccgtcg atcagcattt gaaaggcttg gaagcgatgg
     5761 gtgctgagat tgttatcgaa cacggttacg tcaaagccaa aggcaaactc aaaggtacgc
     5821 gcgtggcgat ggatgtcgtt accgtcggcg gcacggaaaa cctgctgatg gcggcgacgc
     5881 tggcggaagg tacgacggtt ttggaaaact gcgccattga gcctgaagtg gtcgatttgg
     5941 cggaatgcct ggtcaaaatg ggcgcgaaaa tcagcggcat cggtacgtcc acgatggttg
     6001 tggaaggcgc gggcgagcta cacggctgcg aacacagcgt cgtccccgac cggattgaag
     6061 cggggacgtt tctgtgcgcg gtggcgataa ccggcggcag ggtggttttg cggaatgccg
     6121 ccccgaaaac gatggaagtg gtgttggaca aactggttga ggcaggtgcg gtgattgagg
     6181 cgggcgacga ttggatcgcc atcgatatgc ggcagcgtcc gaaggcggtg gacatccgca
     6241 cggtcgtcca ccccggcttc cccacggata tgcaggcgca gtttatggca ttgaatgccg
     6301 tggcggaggg aagctgccgc gtggtggaaa cgatttttga aaaccgcttt atgcacgtcc
     6361 ccgagttgaa ccggatgggg gcgaacatta caaccgaggg caatacggca tttgtgcagg
     6421 gtgtggaacg gctttccggc gcagtcgtca aggcgacgga tttgcgtgcg tccgcaagcc
     6481 tcgttatcgc cggtttggcg gcgcgcggcg aaaccgtggt cgaacagatt taccacttgg
     6541 atcgcggtta tgaaaatatt gaaaaaaaac tcggcagcgt cggcgcgaaa atcgagcgcg
     6601 tatccggctg atcgggttgc gatgccgtct gaagccttca gacg
//
LOCUS       DAXHPG010000117         6633 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXHPG010000117 DAXHPG010000000
VERSION     DAXHPG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.117"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            111..644
                     /locus_tag="ACFM6F_001619"
                     /pseudo
     CDS             111..644
                     /locus_tag="ACFM6F_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
     gene            706..2001
                     /gene="tyrS"
                     /locus_tag="ACFM6F_001620"
     CDS             706..2001
                     /gene="tyrS"
                     /locus_tag="ACFM6F_001620"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC6165006.1"
                     /translation="MSVIQDLQSRDLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            2528..3448
                     /gene="ribF"
                     /locus_tag="ACFM6F_001621"
     CDS             2528..3448
                     /gene="ribF"
                     /locus_tag="ACFM6F_001621"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC6165007.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFASRTGRVPPCRISPLRTKLELLEGTGCIDAVWVLRFDRHF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIAADMEAAKCW"
     gene            3590..6379
                     /gene="ileS"
                     /locus_tag="ACFM6F_001622"
     CDS             3590..6379
                     /gene="ileS"
                     /locus_tag="ACFM6F_001622"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC6165008.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPTGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETPRVAGMRVWEANPVILQWLEETSNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCENYDKLSDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARQLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1620 a         1868 c         1739 g         1406 t
ORIGIN      
        1 gattaagggg gtattggggt aaaattagga ggtatttggt acgaaaacag ccgaaatcct
       61 gtgtttgggt ttcggctgtc gggagggaaa ggaattttgc aaaggtctca ggctacgcgt
      121 cccgcgtgtt ccaaaactgg gccgcctgcc gcgccgttta ccgctatgcc gaagaacacc
      181 tgccccggta ttcaaaagtg gttttggcga tgcgctgggg cagccagatg cccgaaaaca
      241 gccgctccct tgcctacgat gccggttttt tccaaaaatt cgaccgtatg ctgcacaaac
      301 tctcatccga aaaacaagcc gtttacctga tggcggacaa ccttgcctcg tcttacaacg
      361 tccagcgcgc ctatatcttg tcttcacgca taccgggtta ccgccaaacc ctgcgcccgg
      421 acgacgaaag caccctgaaa gccaatgcac gcatcaggga attggcagcc aaatacccca
      481 acgtctatat tattgatgcc gccgcctata tccccgcaga ttttcaaatc ggcggattgc
      541 cggtttactc ggacacagac cacatcaacc cttacggcgg cacggaattg gcaaagcgtt
      601 tttccgaaaa acaacggttt ctcaatacgc gccataacca ttgattcgct taaatttgtt
      661 acaatcggcg gtttgcaaaa acgctaattt ttttgaaaga gaccgatgag cgtcatccaa
      721 gacctgcaat cgcgcgacct tatcgcgcaa acgaccgaca tcgaagcctt agacgctttg
      781 ttgaacgaac aaaaaatcgc cctttactgc ggtttcgatc cgaccgccga cagcctgcac
      841 atcggacacc tgctgcccgt attggcattg cgccgcttcc aacaggcggg gcatacgccg
      901 attgctttgg tgggcggcgc gaccggtatg atcggcgacc ccagcttcaa agccgccgaa
      961 cgcagcttga attccgccga aactgttgcc ggctgggtgg aaagtatccg caaccaatta
     1021 acccctttct tgagctttga aggcggaaat gccgccatta tggcgaacaa tgccgactgg
     1081 ttcggcagca tgaactgcct tgacttcctg cgcgacatcg gcaagcattt ctccgtcaac
     1141 gccatgctga acaaagaatc cgtcaaacag cgcattgacc gcgacggcgc aggcatttcc
     1201 ttcaccgagt tcgcctactc cctgctgcaa ggctacgact tcgccgagtt gaacaaacgc
     1261 cacggcgcgg ttttggaaat cggcggctcc gaccaatggg gcaacatcac cgccggtatc
     1321 gacctgaccc gccgcctgaa tcaaaaacaa gtgttcggcc tgaccctgcc tttggttacc
     1381 aaatcagacg gcaccaaatt cggcaaaacc gaaggcggcg cagtatggct gaacgcgaaa
     1441 aaaacctcgc cctatcagtt ctaccaattc tggctgaaag tcgccgatgc cgatgtgtat
     1501 aaattcctga aatactttac cttcctgtcc atcgaagaaa tcgatgccgt cgaagcgaaa
     1561 gacaaggcaa gcggcagcaa gcccgaagcg caacgcatcc tcgccgaaga aatgacccgc
     1621 ctgattcacg gcgaagaagc ccttgccgcc gcgcagcgca tttccgaaag cctgtttgcc
     1681 gaagaccaaa gcagcctgac cgaaagcgac ttcgagcagc tcgccctcga cggcctgcct
     1741 gcatttgaag tttcagacgg catcaatgtc gtcgaagcct tggtcaaaac cggtttggca
     1801 tcctccaata aagaagcgcg cggctttgtg aacagcaaag cggttttgct caacggcaaa
     1861 cctgccgaag ccaacaaccc caaccacgcc gccgaacgcc ccgacgatgc ctgcctgctg
     1921 accgacgaac acaaacgttt cggcaaatac accatccttc ggcgcggcaa acgcaatcac
     1981 gcgcttttgg tttggaaata atccgattgc cgcagaaatg ccgtcattcc cgcgcaggcg
     2041 ggaatccgga cctgtccgca cggaaactta tcgggcaaaa cggtttctta gattccacgt
     2101 tctagattcc cgcctgagcg ggaatgacga gtttcaagat tacggtgttg tcggaacgca
     2161 actgaaccgt cattcccacg aaagtgggaa tctagaatct cggggtttga gcaactgttt
     2221 ttatccgata agtttctgtg cggacaggtc cggattcccg cctgcgcggg aatgacggcg
     2281 gagggttgtt tgtctcggtt tacctggtta aaaaagaacg attttcactg atgttgcatc
     2341 aggtttgggg cgatgtttca acacatagca ccgcgcctgc tgcgcgcttt atgcgtttgg
     2401 cgcgttcggc tgcgggaaat ttgcctactt ttcccgcgtc gggcgggcgt aacgggcggc
     2461 acactgtcta taaaccgcaa taccgtttac aatgaccgcc tgtttcacca catacccgaa
     2521 cataacaatg agaatcaggc tggggcggca caacgcgccc gactttccgc acggggccgc
     2581 cgtaaccata ggcaattttg acggcgtaca cctcggacac aaacacatcc tccaaaaact
     2641 ccgcctcgaa gccgatgcgc gcggcctgcc cgtcgtagcc gttatcttcg agccgcaacc
     2701 caaagaattt tttgcaagcc gcacgggtag agtgccgccg tgccgcatca gccccctgcg
     2761 taccaaactg gaattgctgg aagggacggg ctgcatcgat gccgtctggg ttttgcgttt
     2821 cgaccggcat ttttccgaaa tatccgcgca agggtttatc gaccgcctgc tgcgtcaaac
     2881 tttaaatacg cgctatttgc tcgtcggcga cgatttccgt ttcggtgcgg ggcgggaagg
     2941 ctgttttgaa ctgttggcac aacagcccga tatgcagacc gaacgcacgc cttccgtcat
     3001 cgtcgaagac atccgcacca gcagtaccgc cgtccgccag gccctttcag acggcaacct
     3061 tgcctatgcg aaaaaacttt tggggcacga ctacgttttg gggggcaggg tggtgcacgg
     3121 cagaaaactc ggacgcacct taaacgcccc gactgccaac atccgcctgc ccggccaccg
     3181 ttatgcactc ggcggcgtgt tcgtcgtcga agcggacggc gcattcggca cgcggcgcgg
     3241 cgtggcgagc ttcggcttca atcccaccgt tgatagcggc tgttctcaaa agcttgaagt
     3301 ccacctgttc gactttcaag gcgacctgta cggacaacgg ctgaacgtcc gcttcctgca
     3361 caaactgcgc gatgaggaaa agtttgacgg tatggaagaa ctgaaaaggc agatagcagc
     3421 cgatatggaa gccgcaaagt gttggtaggc aaaccttata caaaccatcc gattgggcta
     3481 caatcaacct tttaactttt cagacggcat aggggtttcc cgttgtgaaa tactgtttga
     3541 ggcgcaatgc cgtctgaaac cgaaatattg taacaataga gatcaaaaaa tgaccgatta
     3601 cagcaaaacc gtcaacctgc tcgaaagccc gtttccgatg cgcggcaatc ttgccaagcg
     3661 cgagcctgca tggctgaaaa gctggtacga gcaaaaacgc taccaaaaac tgcgcgaaat
     3721 cgccaaaggc cgtccgaaat tcatcctgca cgacggcccg ccgtatgcca acggcgacat
     3781 ccacatcggt cacgccgtca acaaaattct caaagacatc attatccgca gcaaaaccca
     3841 agccggtttt gacgcgcctt atgtgccggg ttgggactgt cacggcctgc ctatcgaagt
     3901 gatggtggaa aaactgcacg gcaaagacat gcctaaagcc cgtttccgcg aattgtgccg
     3961 cgaatacgcc gccgaacaga ttgcccgtca gaaaaaagac tttatccgct tgggcgtgtt
     4021 gggtgactgg gaccatcctt acctgaccat ggatttcaaa acagaagccg ataccgtgcg
     4081 tatgctcggc gaaatctaca aatcgggcta tctctaccgg ggcgcgaaac cggttcaatt
     4141 ctgcttggac tgcggttctt cattggccga agcggaagtg gaatacaaag acaaagtatc
     4201 gcctgcgatt gacgttgcct atccgtttaa agacaccgcc gcgcttgccg ccgcattcgg
     4261 tttggcaggt atcgaaggca aagcgtttgc cgttatttgg acaaccacgc cttggacgct
     4321 gccggcgagc caagccgtgt ctgccggtgc ggacgtggtg tatcaattaa tcgatacgcc
     4381 tacaggcaaa ttggtgctgg cgaaagattt ggcggaagat gcgctcaaac gttacggctt
     4441 ttcagacggc atcgccatcc ttgccgaaac caccggcgac aagctggaaa acctgcacat
     4501 gaaccatccg ttcctcgaac gcgatattcc catgcttaac ggcgaacacg ttaccaccga
     4561 tgccggtacc ggcttggtgc atactgcgcc cgcgcacggt ttggaagact acgccgtctg
     4621 caacaaatac ggcatcgagc tttacaatcc tgtcaacgcc gaaggcaaat acatcggcga
     4681 aacgccgcgt gtcgccggta tgcgcgtttg ggaggcgaac cccgtcatcc tgcaatggtt
     4741 ggaagaaacc agcaaccttt tggcaagcag taaaatcgaa cacagctacg cccactgctg
     4801 gcggcacaaa acgccgctga tttaccgcgc gacaggtcaa tggtttgtcg gtatggacaa
     4861 agccggtgcc gacggcaaaa ccctgcgcga caaagccatc aaggccgtgg acgacaccga
     4921 attcttcccg tcttggggtc gcgcgcgttt ggaagccatg attgaaggtc gtcctgactg
     4981 ggtggtttca cgccaacgct attggggcac gccgatgact ttctttgttc acaaagaaac
     5041 gggcgagctg catccgaact ctgccgaact tttggaaaaa gttgccctga aaatcgaaga
     5101 aaaaggcatc gaagcgtggt tctccctcga taagagcgaa ctcttgagcg cggaagattg
     5161 cgaaaattac gataaacttt ctgacacaat ggacgtatgg ttcgattccg gttcgaccca
     5221 ttattccgtt gtgaaacaac gcgaagaatt ggaatggccg gcggatttgt atctcgaagg
     5281 cagcgaccag caccgcggct ggtttcaatc gtccatgctg accggctgcg cctcatcaat
     5341 gggtcgcgcg ccgtataaac agctgctgac ccatggtttc gttgtcgacg gcgaaggcaa
     5401 aaaaatgtcg aaatccatcg gcaacgtcgt tgcaccgcaa gaggtttata acgaattcgg
     5461 cgcagacatc ctgcgcctgt gggcggcatc taccgattac agcggcgaat tggcgatttc
     5521 caaagaaatc ctcaaacgcg taaccgaaag ctaccgccgt atccgcaata ccttgagctt
     5581 tttgtttgcc aacttgagcg actttaatcc gattgaagat gccgtgcaac aggcggatat
     5641 ggtggaaatc gaccgctacg ccgtggtatt ggcacgtcag ctgcaagagc gtctggcagg
     5701 cgattactat ccgcgttatg ccttccactt tgccgtaaaa gacattgttt ctttctgctc
     5761 ggaagacttg ggtgcgttct acctcgacat cctgaaagac cgcctctaca ccaccaaagc
     5821 cgacagccat gcacgccgca gcgcacaaac tgccctgtat cacatcacac gcagtttggt
     5881 tctcttgatt gcaccgattt tgtgcttcac cggcgaagaa gcgtgggaca tcatcggcgg
     5941 cggcgaagaa gacagcgtcc tcttccatac ttggcacgag ttcccgacca tcaacgaaaa
     6001 aaccgaagcc gaactggtga aaaaatggac ggcaatccgc gaagcccgcg aagcggtaac
     6061 cgccgccatc gagcctttgc gcgccgacaa aaccgtcggt tcgtccttgc aagccgaagc
     6121 cgaaattacc gcgccggaag aaatggccgg ctatctgaat gctttgggcg aagaattgcg
     6181 ctttgctttg ctggtgtcta aagcagaagt gaaagtaggc agcgaacttg ccgttgccgc
     6241 taaagccagt gatggtgaaa aatgcgaacg ctgctggcac tacacccgcg atgtgggcgc
     6301 ggttgcaggc tatgaaaccg tctgcaaacg ctgtgcagag aatgtcggcg gagaaggcga
     6361 aacgcgccat tacgcctgat aaagtttgag caaatgccgt ctgaaaccgc cgaacagcat
     6421 ttcagacggc attttttgtg ccgcgatttg tctttataac ggcggagggg tttcaagatt
     6481 gcggtgttgt cggaatgcaa ctgaaccgtc attcccacga aagtgggaat ctagaatctc
     6541 gaggtttcag tcatttccga tagattcccg ccgcgtcgtg ggtctggatt cccgcctgtg
     6601 tgggaatgac ggatttcgag attgcggtgt tgt
//
LOCUS       DAXHPG010000118         6599 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXHPG010000118 DAXHPG010000000
VERSION     DAXHPG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.118"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            335..2164
                     /locus_tag="ACFM6F_001623"
     CDS             335..2164
                     /locus_tag="ACFM6F_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC6165009.1"
                     /translation="MMIKPTALLLPALFFFPHTYAPAADLSENKAAGFALFKNKSPDT
                     ESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAPDNVKTM
                     LHSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEYYRNALE
                     NWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADLNVVVDS
                     GRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHYSGASVQ
                     ADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKGYIGSVV
                     WDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVRDRAGID
                     ARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDGKIGTTL
                     GTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTLARDNADVPSGLMFRSGGA
                     SSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDAAANFKR
                     MKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            2219..6388
                     /locus_tag="ACFM6F_001624"
     CDS             2219..6388
                     /locus_tag="ACFM6F_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC6165010.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDLFKTGKISVGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNVSSNYGKINGNITVGQSNSFSTSPLGGKLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGIENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
BASE COUNT         1568 a         2112 c         1721 g         1198 t
ORIGIN      
        1 ttgcgttgat ttgccctatg cagtgccgga caggctttgc tttatcattc ggcgcaacgg
       61 tttaatttat tgaacgaaaa taaatttatt taatcctgcc tattttccgg cactattccg
      121 aaacgcagcc tgttttccat atgcggattg gaaacaaaat accttaaaac aagcagatac
      181 atttccggag ggccgcaacc tccgaaatac cggcggcagt atgccgtctg aagcgtcccg
      241 ccccgtccga acaacacaaa aacggccgtt cgaaaccctg tccgaatagt gttagaatcg
      301 aaatctgcca caccgagtgc acgacacccg taccatgatg attaaaccga ccgccctgct
      361 cctgccggct ttatttttct ttccgcacac atacgcgcct gccgccgacc tttccgaaaa
      421 caaggcggcg ggtttcgcat tgttcaaaaa caaaagcccc gacaccgaat cagttaaatt
      481 aaaacccaaa ttccccgtcc gcatcgacac gcaggatagt gaaatcaaag atatggtcga
      541 agaacacctg ccgctcatca cgcagcagca ggaagaagta ttggacaagg aacagacggg
      601 cttcctcgcc gaagaagcac cggacaacgt taaaacaatg ctccacagca aaggctattt
      661 cagcagcaaa gtcagcctga cggaaaaaga cggagcttat acggtacaca tcacaccggg
      721 cccgcgcacc aaaatcgcca acgtcggcgt cgccatcctc ggcgacatcc tttcagacgg
      781 caacctcgcc gaatactacc gcaacgcgct ggaaaactgg cagcagccgg taggcagtga
      841 tttcgatcag gacagttggg aaaacagcaa aacttccgtc ctcggcgcgg taacgcgcaa
      901 aggctacccg cttgccaagc tcggcaacac ccgggcggcc gtcaaccccg ataccgccac
      961 cgccgatttg aacgtcgtcg tggacagcgg ccgccccatc gccttcggcg actttgaaat
     1021 caccggcaca cagcgttacc ccgaacaaat cgtctccggc ctggcgcgct tccaaccggg
     1081 cacgccctac gacctcgacc tgctgctcga cttccaacag gcactcgaac aaaacgggca
     1141 ttattccggc gcgtccgtac aagccgactt cgaccgtctc caaggcgacc gcgtccccgt
     1201 caaagtcagc gtaaccgagg tcaaacgcca caagctcgaa accggcatcc gcctcgattc
     1261 ggaatacggt ttgggcggca aaatcgccta cgactattac aacctcttca acaaaggcta
     1321 tatcggctcg gtcgtctggg atatggacaa atacgaaacc acgcttgccg ccggcatcag
     1381 ccagccgcgc aactatcggg gcaactactg gacaagcaac gtttcctaca accgttcgac
     1441 cacccaaaac ctcgaaaaac gcgccttctc cggcggcatc tggtatgtgc gcgaccgcgc
     1501 gggcatcgat gccaggctgg gggcagagtt tctcgcagaa ggccggaaaa ttcccggctc
     1561 ggatatcgat ttgggcaaca gccacgccac gatgctgacc gcctcttgga aacgccagct
     1621 gctcaacaac gtgctgcatc ccgaaaacgg ccattacctc gacggcaaaa tcggtacgac
     1681 tttgggcaca ttcctgtcct ccaccgcgct gatccgcacc tctgcccgcg caggttattt
     1741 cttcacgccc gaaaacaaga aactcggcac gttcatcata cgcggacaag cgggttacac
     1801 ccttgcccgc gacaatgccg atgtcccctc ggggctgatg ttccgcagcg gtggcgcgtc
     1861 ttccgtgcgc ggttacgaac tcgacagcat cggacttgcc ggcccgaacg gatcggtcct
     1921 acccgaacgc gccctcttgg tgggcagcct ggaataccaa ctaccgttta cgcgcaccct
     1981 ttccggcgcg gtgttccacg atatgggcga tgccgccgcc aatttcaaac gtatgaagct
     2041 gaaacacggt tcgggactgg gcgtacgctg gttcagccca ctcgcgccgt tttccttcga
     2101 catcgcctac gggcacagcg acaagaaaat ccgctggcac atcagcctgg gaacgcgctt
     2161 ctaaaccgat acggccgctt cagacggcat tgcagcaaac tattttgaaa cagacattat
     2221 gaccgatacc gcaccgacag ataccgatcc gaccgaaaac ggcacgcgca aaatgccgtc
     2281 tgaacaccgc cccaccccgc cggcaaaaaa acgccgcccg ctgctgaagc tgtcggcggc
     2341 actgctgtct gtcctgattt tggcagtatg tttcctcggc tggctcgccg gtacggaagc
     2401 gggtttgcgc ttcgggctgt accaaatccc gtcttggttc ggcgtaaaca tttcctccca
     2461 aaatctcaaa ggcacgctgc tcgacggctt cgacggcgac aactggtcga tagaaaccga
     2521 gggggcagac cttaaaatca gccgcttccg cttcgcgtgg aaaccgtccg aactgatgcg
     2581 ccgcagcctg cacatcaccg aaatttccgc cggcgacatc gccatcgtta ccaaaccgac
     2641 tccgcctaaa gaagaacgcc ctccgctcag ccttcccgac agcatagacc tgcctgccgc
     2701 cgtctatctc gacctcttca agacgggcaa aatcagcgtc ggcaaagcct ttgacaaaca
     2761 aaccgtctat ctcgaacggc tggatgcttc ataccgttac gaccgcaaag gacaccgcct
     2821 tgacctgaag gccgccgaca cgccgtggag cagttcgtcg ggggcggcct cggtcggctt
     2881 gaaaaaaccg tttgccctcg ataccgccat ttacaccaaa ggcggactcg aaggcaaaac
     2941 catacacagt acggctcggc tgagcggcag cctgaaggat gtgcgcgccg aactggcgat
     3001 cgacggcggc aatatccgcc tctcgggaaa atccgtcatc cacccgtttg ccgaatcatt
     3061 ggataaaaca ttggaagaag tactggtcaa agggttcaac atcaatccgt ccgccttcgt
     3121 gccttccctg cccgatgccg ggctgaattt cgacctgacc gccatcccgt cgttttcaga
     3181 cggcatcgcg ctggaaggct cgctcgattt ggaaaacact aaagccggtt ttgccgaccg
     3241 caacggcatc cccgtccgtc aggttttagg cagctttgtc atccggcagg acggcacggt
     3301 gcatatcggc aatacgtccg ccgccctgct cggacggggc ggcatcaggc tgtcgggcaa
     3361 aatcgacacc gaaaaagaca tcctcgattt aaatataggc atcaactccg tcggcgcgga
     3421 agacgtactg caaaccgcgt tcaaaggcag gttggacggc agcatcggca tcggtggcac
     3481 gaccgcctcg cccaaaatct cttggcaact cggcatcggc acggcgcgca cggacggcag
     3541 cctcgccatt gcaagcgacc ccgcaaacgg acagcggaaa ctggtgctcg acaccgtcaa
     3601 catcgccgcc gggcaaggca gcctgaccgc gcaaggctat ctcgagctgt ttaaagaccg
     3661 cctgctcaag ctggacatcc gttcccgcgc attcgaccct tcgcgcatcg atccgcaact
     3721 tccggcaggc aatatcaacg gctcaataaa ccttgccggc gaactggcaa aagagaaatt
     3781 cacaggcaaa atgcggtttt tacccggcac gttcaacggc gtaccgattg ccggcagtgc
     3841 cgacattgtt tacgagtccc gccaccttcc gcgtgccgcc gtcgatttgc ggctggggcg
     3901 gaacatcatc aaaacagacg gcggcttcgg caaaaaaggc gaccggctta acctcaatat
     3961 caccgcaccc gatttatccc gtttcggttt cggactcgcg gggtctttaa atgtacgcgg
     4021 acacctttcc ggcgatttgg acggcggcat ccgaaccttt gaaaccgacc tttccggcgc
     4081 ggcgcgcaac ctgcacatcg gcaaggcggc agacatccgt tcgctcgatt tcacgctcaa
     4141 aggttcgccc gacacaagcc gcccgatacg cgccgacatc aaaggcagcc gcctttcgct
     4201 gtcgggcgga gcggcggttg tcgataccgc cgacctgatg ctggacggca cgggcgtgca
     4261 gcaccgcatc cgcacacacg ccgccatgac gctggatggc aaaccgttca aattcgattt
     4321 ggacgcttca ggcggcatca acagggaact tacccgatgg aaaggcagca tcggcatcct
     4381 cgacatcggc ggcgcattca acctcaagct gcaaaaccgt atgacgctcg aagccggtgc
     4441 ggaacgcgtg gcggcaagtg cggcaaattg gcaggcaatg ggcggcagcc tcaacctgca
     4501 acacttttct tgggataaaa aaaccggcat atcggcaaaa ggcggcgcac acggtctgca
     4561 tatcgccgag ttgcacaatt tcttcaaacc gcccttcgaa cacaatctgg ttttaaacgg
     4621 cgactgggat gttgcctacg ggcgcaacgc gcgcggctac ctcaatatca gccggcaaag
     4681 cggcgatgcc gtattgcccg gcgggcaggc tttgggtttg aacgcatttt ccctgaaaac
     4741 gcgctttcaa aacgaccgta tcggaatcct gcttgacggc ggcgcgcgtt tcgggcggat
     4801 taacgccgat ttggacatcg gcaacgcctt cggcggcaat atggcaaatg caccgctcgg
     4861 cggcaggatt accgcctccc ttcccgactt gggcacattg aagccctttc tgcccgccgc
     4921 cgcgcaaaac attaccggca gcctgaatgc cgccgcgcaa atcggcggac gggtcggctc
     4981 tccgtccgtc aatgccgccg tcaacgtcag cagcaactac gggaaaatca acggcaacat
     5041 caccgtcggg caaagtaatt ctttcagtac ctcgcccctg ggcgggaaac tcaacctgac
     5101 cgttgccgat gccgaagtat tccgcaactt cctgcccgtc ggacaaaccg tcaaaggcag
     5161 cctgaatgcc gccgtaaccc tcggcggcag catcgccgat ccgcacttgg gcggcagcat
     5221 caacggcgac aaactctatt accgcaacca aacccaaggc atcatcttgg acaacggctc
     5281 gctgcgttcg catatcgcgg gcaggaaatg ggtaatcgac agcctgaaat tccggcacga
     5341 agggacggcg gaactctccg gtacggtcgg tatagaaaac agcggacccg atgtcgatat
     5401 cggcgcggtg ttcgacaaat accgcatcct gtcccgcccc aaccgccgcc tgacggtttc
     5461 cggcaacacc cgcctgcgct attcgccgca aaaaggcata tccgttaccg ggatgattaa
     5521 aacggatcag gggctgttcg gttcgcaaaa atcctcgatg ccgtccgtcg gcgacgatgt
     5581 cgtcgtatta ggcgaagtca aaaaagaggc ggcggcaccg ctccccgtca atatgaacct
     5641 gactttagac ctcaatgacg gcatccgctt cgccggctac ggcgcggacg ttaccatagg
     5701 cggcaaactg accctgaccg cccaatcggg cggaagcgtg cggggcgtgg gcacggtccg
     5761 cgtcatcaaa gggcgttata aggcatacgg gcaggatttg gacattacca aaggcacggt
     5821 ctcctttgtc ggcccgctca acgaccccaa cctcaacatc cgcgccgaac gccgcctttc
     5881 ccccgtcggt gcgggcgtgg aaatattggg cagcctcaac agtccgcgca ttacgctgac
     5941 ggcaaacgaa ccgatgagtg aaaaagacaa gctctcctgg ctcatcctca accgcgccgg
     6001 cagtggcagc agcggcgaca atgccgccct gtccgcagcc gctggcgcgc tgcttgccgg
     6061 gcaaatcaac gaccgcatcg ggctggtgga tgatttgggc tttaccagca agcgcagccg
     6121 caacgcgcaa accggcgaac tcaaccccgc cgaacaggtg ctgaccgtcg gcaaacaact
     6181 gaccggcaaa ctctacatcg gctacgaata cagcatctcc agcgcggaac agtccgtcaa
     6241 actgatttac cggctgaccc gcgccataca ggcggttgcc cgtatcggta gccgttcgtc
     6301 gggcggcgag ctgacataca ccatacgttt cgaccgcttc tccggttcgg acaaaaaaga
     6361 ctccgccgga aacagcaaag gaaaataagc ggttttcaga cggcgcgccg ccaaaccgga
     6421 catttgaaaa cctgcttttc caccgtccgc cgccgccgtc cgcctgcaag ggaacagaat
     6481 cgatatagtg aattaacaaa aatcaggata aggcgacgaa gccgcagaca gtacaaatag
     6541 tacggaaccg attcactcgg tgcttgagca ccttagagaa tcgttctctt tgagctaag
//
LOCUS       DAXHPG010000119         6479 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXHPG010000119 DAXHPG010000000
VERSION     DAXHPG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.119"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..336
                     /locus_tag="ACFM6F_001625"
     CDS             <1..336
                     /locus_tag="ACFM6F_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC6165011.1"
                     /translation="IDASGLGEKIKDGKKTVLSCEEEQKICNTFTNKQAVEDFSVVIG
                     YDEIKAKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKD
                     RLAMLKFNS"
     gene            368..484
                     /locus_tag="ACFM6F_001626"
     CDS             368..484
                     /locus_tag="ACFM6F_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6165012.1"
                     /translation="MPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYRK"
     gene            complement(644..1981)
                     /gene="ribBA"
                     /locus_tag="ACFM6F_001627"
     CDS             complement(644..1981)
                     /gene="ribBA"
                     /locus_tag="ACFM6F_001627"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC6165013.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGAALLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            2090..2224
                     /locus_tag="ACFM6F_001628"
     CDS             2090..2224
                     /locus_tag="ACFM6F_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165014.1"
                     /translation="MPSEAGFVASDGIAAILAGRGLFVPTSRALLIWGLPEAYSEKNF
                     "
     gene            complement(2203..3216)
                     /locus_tag="ACFM6F_001629"
     CDS             complement(2203..3216)
                     /locus_tag="ACFM6F_001629"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6165015.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKNA
                     GGGILFDLQQLADKKVDLILHPSSLNYHVIPKGADFSDNDFVRHFETLVEGRYYIANA
                     WTKIVRREIIIKNNLFFPKGYIHEDFPYSLKLARFVKTFAFYDNPFYQYRVLGNSISH
                     NVKYKNFSDMLAHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNII
                     LIYRKYFSFKEKCRKIFGAKTIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKF
                     FSE"
     gene            complement(3372..3965)
                     /gene="ribA"
                     /locus_tag="ACFM6F_001630"
     CDS             complement(3372..3965)
                     /gene="ribA"
                     /locus_tag="ACFM6F_001630"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC6165016.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3958..4632)
                     /locus_tag="ACFM6F_001631"
     CDS             complement(3958..4632)
                     /locus_tag="ACFM6F_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165017.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRTAYLTAQTVMDMGTTSIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            4786..4870
                     /locus_tag="ACFM6F_001632"
     tRNA            4786..4870
                     /locus_tag="ACFM6F_001632"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4820..4822,aa:Leu,seq:tag)
     gene            5390..6424
                     /gene="purM"
                     /locus_tag="ACFM6F_001633"
     CDS             5390..6424
                     /gene="purM"
                     /locus_tag="ACFM6F_001633"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC6165018.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDMVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         1613 a         1571 c         1687 g         1608 t
ORIGIN      
        1 atcgacgcat cgggcttggg cgaaaaaatt aaagacggca aaaaaaccgt actttcctgc
       61 gaagaagaac aaaaaatctg caatactttc acgaacaaac aggcagtgga agatttcagt
      121 gtggtaatcg gctacgatga aatcaaagcg aagaatcaca gcttgtcggc ggggcagtat
      181 ttcgaggtaa agattgatta tgtggatatt tccgccgacg aatttgcgca aaaaatggcg
      241 ggattttcag cggatttgga taaacttttc gccgaatctg ccgaattgga aaaagagatt
      301 aaggatagat tggcgatgtt gaaattcaat tcataaatac cgatgaatga aaccggtcaa
      361 acttaaaatg ccgtctgaaa gcctttcaga cggcattttg tttggtttgg aaaatactta
      421 tgccgttctt tccctgaaag agagaatcca aaaaaccaaa gccacaggaa tttatcggaa
      481 atgacaaaaa cccgacgaac cggattcccg cctgcgcggg aatgacgaat tagaagttac
      541 ccgaaatttg caaaaacaaa atccaaccca acagaccggg ttttcgtttg cacggaaata
      601 atgcaataaa taaagcaaat ataaagtatt tgaatttact atattatttt tccgcttctt
      661 caaagccgac gacttccaaa ccgaagccgg tcaggccggt gaaagatgag ggctgcccga
      721 ggacgcgcag ttttttgacg ttgaggccgg cgaggatttg cgcaccgatg ccgtagcttt
      781 tgctgtccca tttgtaggct tggtttgcac ctttgggcag ggtgcggtcg agcagggcgg
      841 cgccgtcttc ggtgcggtgc agaaggatga ctacgccgct ttcggcctgt tggatgcgct
      901 cgagtgcttt gggcagcgac catgaatgac gcgggttagc ttggatgaag tccatgacgc
      961 tgaagggttc gtggacgcgg acgagggttt cggtgtcggc ggagggcgtg cctttgacga
     1021 gggcgaggtg ggtttcgccg gagagtttgt cgacgtaaac gtgttgttgg aactcgcccc
     1081 acggggtttg tacaggcgca ttgcccatgt cttcaagcag gctttcggta cggctgcggt
     1141 attcgatgag gtcggcaatc gtgccgattt tgagcttgtg ttcttcggcg aatttcatca
     1201 gttcgggcat acgcgccatc gtgccgtcgt cgttgatgat ttcgcaaata acggaggcag
     1261 gaatcagccc gttcatttgc gccaggtcga cgccggcttc ggtgtgtccg gcgcggacga
     1321 gtacgccgcc tttttgggcg cgaagcggaa agatatgacc gggttggacg atgtcttcgg
     1381 gtttagcggt cggggaaacg gcggtttgaa tagtcagggc gcggtcggcg gcggaaatgc
     1441 cggtggtaat gccgtgtgcg gcttcgatgg agacagtaaa gttggtgccg tattgcgcgc
     1501 cgtttttttg ggtcatcatc ggcagcccga gtttttcgac catttcgccg tccatcggca
     1561 ggcagaccaa gccgcgcgcg tgtttgatca tgaagttgat ggcttcgggc gtgacgaatt
     1621 gcgccgccat cagcaggtcg ccttcgtttt ctcggtcttc ggcatcggtg atgatgacca
     1681 ttttgccggc tttgatgtcg gctaggattt cggggatggg ggaaatatgg gacatggtgt
     1741 gttttcctgt gtgttcgggc gggcgggcgg tgtgttcggt atcgagccag aaggcgggca
     1801 tttttgccgc ctgttcgatt ttacgggctt ttttctcgcc gaaggatttg tttttcaaaa
     1861 gggcggagag ttcgcctgtg ttgagggcaa cggcttcggc aaaacgggtt tgcaaaccgc
     1921 cgtagtgttc tgctatccat tgccgcaggt tgcggcggcg cagaccggct gtgtcggtca
     1981 tcgtttcatc ctttgtagaa atcggatgta gcggattgta atcgcgtatt taccaaaaag
     2041 caaatctgtt tttttcgcca aaccgaatta tttgcttttt ggtaaacaga tgccgtctga
     2101 agccggattc gtggcttcag acggcattgc cgcaatcctt gccggccggg ggctgtttgt
     2161 cccaacttcc cgcgcacttc tgatttgggg tttgccggaa gcttattccg aaaaaaattt
     2221 ctgataaacg gccttgattg ccgggtacag cataggcggt acgagcaggc gcaatatcgg
     2281 caatcctatg atgaatgcgg tcttcccgat aaaaaccgga cggattgtct tcgcgccgaa
     2341 tatctttctg catttttctt taaatgaaaa atatttccgg tagatgagga taatgttttt
     2401 ggaaaaataa agccttacca atatagacct cagatagccg atattgtcga agacaaattt
     2461 ttgcaaaccg ccgtagatgg gggaattttt gttttcgact aaaaaatcca caccccggtc
     2521 gagatgcgcc agcatatcgc tgaaattttt gtatttgacg ttgtggctga tagagttgcc
     2581 gagaacgcgg tattgataaa aagggttatc gtaaaaggca aaagtcttga caaaacgcgc
     2641 caatttcaaa ctgtacggga aatcctcgtg aatgtatcct tttgggaaaa acagattgtt
     2701 tttgatgatt atttcccgcc tgacgatttt tgtccacgcg ttggcgatat agtaccgccc
     2761 ctccaccaac gtttcaaaat ggcggacaaa atcattatcc gaaaagtccg cccctttggg
     2821 gatgacgtgg taattgaggg acgagggatg caggatcaaa tcaacctttt tgtctgcaag
     2881 ttgttgtaaa tcaaagagaa tccccccccc cgcgtttttt gaacggttgg tatcggccca
     2941 ataatcgtcg ctgtccaaaa agattaggta atcgcctttt gccgcccgga taccggcgtt
     3001 gcgggcatcc gacagcccgc cgttttcttg atgaatcact tttatatgcg gatatttgcc
     3061 tgcatattcg tcgcaaatct tcccgcagcc gtccggcgaa ccgtcatcga ccaaaatcat
     3121 ttcataatcg gcaaaatttt cggcaagcac ggaatccacg cagcagcgga ggtatttttc
     3181 cacattgtaa atagggacga tgatggagaa aatcataaat atcaatccat tatattaaga
     3241 tgtttgcgcg tatgcctcaa acccgcgctc gcaatgcgtt tgcatccact ataaaactta
     3301 ttgctttatc aaggtatgga aacctgtttc ccgaaaggcg gcgcaggatg cccgttccct
     3361 gcaactttgc cttattccga catcagatgt cccagcttgt ctgctttggt ttggaggtag
     3421 cgtttgtttt caaggttttc cccgacgtgc agggggatgc gttcgaccac gttaatcccc
     3481 gcatctttca gggtttggat tttttcgggg ttgttggtca gcagtttgac cgagcggatg
     3541 cccagatatt cgtagataga ttgcgccaaa cggaaatcgc gggcatcgac gggcagcccg
     3601 agtgccaaat tggcttcaac cgtatccata ccttggtctt gcagatgata ggcgcggatt
     3661 ttgttaatca gcccgatgcc gcgtccttcc tgacgcagat agacgatgat gccgcgcccc
     3721 tctgtctgta cggccctcat ggccgcttca agttgaggtc cgcagtcgca ttttctcgag
     3781 aacagcgcat cgcccgtcag gcattcggag tggatgcgcg tcagaaccgg attgccgtct
     3841 gaaaaatcac cgacggtcag cgcgacgtgt tcctgcccat ttgcctcttc aaagccgtgc
     3901 atcgtaaata cgccccattc ggtcggcagg cggcaggaag caacatagtt taataattca
     3961 gacattttca tctccttctg cggcagtgat gcccaaaagc tgcttcaaag gttcggacaa
     4021 agccaaggca agagccaccc attccacctg tgctgccgta cccgcacact ctgcgcattc
     4081 aaattccaca tgaaccacgc ccaacacacc gccactttcc gtgcagaccg gaatggaaat
     4141 ttgcgccgcc gaagcatgat tgcgttctcc cgaaagctct cccaaatcca accaacggcg
     4201 tacatccgag gcaacattca tccagccgct ttgcgccgaa cggcaagcca gcgaaacctt
     4261 gcctgccgct tcatccaaat cccacaggtt ttccaaaacc tcgccctgcc gggacaggca
     4321 aaccagccga aaggcggcat tttcagacgg catcagggca taaacggctg cggcgcgcac
     4381 gcctttacag cgtgaaaaca ccgaatccaa agccatgaaa agccgtttca gaccttcctc
     4441 attcccgctg ttttgctcca aataatcggc aagcctccaa tcctcatgct caagccacaa
     4501 aaccgaacgc tcaatcgaag tcgtccccat atccattacc gtctgcgccg tcagatacgc
     4561 cgtccgaacc tcgtcaagcg gcagcttcaa accctgagtc agcagaaaat ccttaatcaa
     4621 agcagcaggc atacccaaat tccgtcaata aaaacaaaaa accgcccgat tccgatgtca
     4681 ggcggacgaa aaacagattt taccgcccca aaccccaagc catcaacagc cccgccgaaa
     4741 accttgcaca aaaaaacaaa atctgtataa ttgtccaatt cccatgcgga cgtggcgaaa
     4801 ttggtagacg caccagattt aggttctggc gccgagaggt gtgagagttc gagtctctcc
     4861 gtccgcacca ataatgataa ataaatcata tagttaatgc ttatcgggac aaattagtgc
     4921 cacaaaacat aagtcgtctg cttttcagcg ggcggctttt ttatctgcct atatgtttca
     4981 acacgcagaa cggtacataa agtgccgcca tttgttctac cccgaatcag aagcgggtgc
     5041 ttccgcccgc aatcagattc accgccccta atgccggggt ttcaactgga aagaagaaaa
     5101 tattgatttt gatgatggca tcaaaaaacc ccttccgccc gtctgccaaa aggaatggtt
     5161 aagaaaagca atgcgtcttt tgatggaacg cgatatataa ggaattgagg ggaagggcag
     5221 gcgggtttct gcccgttaat cctgcggacg atcccgccaa acccgacacg cgcgggcata
     5281 tggcggattg tttacaatat tgtctaaaaa tctgaacctg caaacgtgaa ataaggtaga
     5341 atacgccccg ttattttacc gtccaatccc catttaccaa ggaaaaacga tgagtacttc
     5401 attgagttac cgcgatgcag gtgtcgatat cgacgcaggc gaccaactgg tcgaaaacat
     5461 caaaccgttt gccaaacgca ccatgcgtcc agaagtattg ggggatttgg gcggttttgg
     5521 cgcattggtc gaaatcggca agaaatataa aaatcccgta ttggtcagcg gtacggacgg
     5581 tgtgggtacc aagctcaagc ttgcctttga ttgggataaa cacgatatgg tgggcatcga
     5641 ccttgttgca atgagtgtca acgatatttt ggttcaagga gcagaacctc tgtttttctt
     5701 ggattatttc gcctgcggca aattggatgt tccgcgcgca accgatgtca tcaaaggcat
     5761 tgcacaaggt tgcgaagaat ccggctgcgc cctgattggc ggggaaactg ccgaaatgcc
     5821 gggcatgtat cccgtcggag aatacgattt ggcgggtttt gccgtcggcg tagtggaaaa
     5881 agagaatgtc attaccggcc gcagtatcgg cgcgggcgat gtggtattgg gtttggcttc
     5941 caacggcgca cattcaaacg gctattccct tatccgtaaa atcatcgaac gcgacaatcc
     6001 cgatttggat gccgagtttg ataatggcaa aaccttgcgg gaggctgtta ttgcgccgac
     6061 ccgtctgtat gtgaaaccga ttcttgccgc tttggaaaaa tttaccatta aaggtatggc
     6121 acacattacc ggcggcggca ttaccgaaaa cgtgccgcgc gtgttgcctg aaaacacggt
     6181 tgcacaaatc gatgctaaat cgtgggaatt gcccaagctc ttccaatggc ttcaaaaggc
     6241 gggcaatgtg gaaacccaag aaatgtaccg aacctttaac tgcggcatcg gcatggtcgt
     6301 tattgttgct gccgaagatg ccgatgcggt tcagggtctc ttgggtgaac aaggtgaaac
     6361 agtttaccgt ttaggtttga tccgtgagcg tcagggagac gagcatcaaa cccaggttgc
     6421 ctgattgctt ctatagcgaa atgccgtctc aaacatgggt ttggacggca tttttatag
//
LOCUS       DAXHPG010000120         6392 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXHPG010000120 DAXHPG010000000
VERSION     DAXHPG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.120"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..316
                     /locus_tag="ACFM6F_001634"
     CDS             <1..316
                     /locus_tag="ACFM6F_001634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165019.1"
                     /translation="KNIPLKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGF
                     NGDIGEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEM
                     ER"
     gene            complement(398..2485)
                     /locus_tag="ACFM6F_001635"
     CDS             complement(398..2485)
                     /locus_tag="ACFM6F_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFC6165020.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2488..3753)
                     /locus_tag="ACFM6F_001636"
     CDS             complement(2488..3753)
                     /locus_tag="ACFM6F_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC6165021.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKLEQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(3797..3934)
                     /locus_tag="ACFM6F_001637"
     CDS             complement(3797..3934)
                     /locus_tag="ACFM6F_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165022.1"
                     /translation="MNTETIYATVFCILAATISGLLVKFNVIKIETSINSKFMLLGIS
                     I"
     gene            complement(4974..5210)
                     /locus_tag="ACFM6F_001638"
     CDS             complement(4974..5210)
                     /locus_tag="ACFM6F_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6165023.1"
                     /translation="MKELHTSELVEVSGGKFHIFAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
     gene            complement(6264..6392)
                     /locus_tag="ACFM6F_001639"
                     /pseudo
     CDS             complement(6264..6392)
                     /locus_tag="ACFM6F_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         2046 a         1120 c         1108 g         2118 t
ORIGIN      
        1 aaaaaatatc cctttgaaag attttgatga agtttgtgaa aagtgggaga aatcctttaa
       61 agaaaatgga aaagaaattg aatataatca aaaatattca agaatttatg gtgaagttcg
      121 tggagctggc tttaatggcg atatcggaga aaagctacat cgttgtgccg tgatggaatg
      181 ggctgaaaat gagaccgtga aattagccca aaaatgggaa caagagcaga aaaaacaaca
      241 aattcaacag aaaaaggaaa ctgaaaaatc gccaaaacac aaagccagtc gtgatgattg
      301 ggaaatggaa cgttaaaccg ctattttcat gtattctatt aaattatttg aaaaaagact
      361 tcactttttg ttaaagaaaa atgaagtctt gttctgttca acctaaattc agaattttat
      421 ctgccgattg aattgtttct tggcggtgtg caaccattat tttcataata ccaagacttt
      481 ttaggttatg gttaattttt tgttcatttt ctacatctaa atggctactt gcttcgtcta
      541 aaaatagaat tttgggtcgt ttatacaatg cacgagccaa gataactctc tgcttttgtc
      601 cacctgataa gatatttccc atatcgccaa tcaaggtctc atagcccatt ggcattttaa
      661 gtatatcgtc atgtatttgt gccatttttg cacattgttc aatgagctcc atatttgggc
      721 tttcatcaaa aaatgaaata ttttccccaa tagaacctgc aaaaaggaca tcatcttggc
      781 gaacaatccc gctcaatccc ctaataaagg atggagaaac ttgatatata tcatgcccat
      841 taatactaac tgtaccagtt tcaggtttta ggctacctgt taaaatgttt aacaaagtgg
      901 acttcccccg accagattgt cctgttaaaa caactgcttc attatcttta aattccaaat
      961 taatgttttc aaaaagatat ggctcattat cagcatatct gaatgagacg ttttcaactt
     1021 taagaaccag ttgttcatta tctaatttag gtatatgatt atacttaata atttcagttt
     1081 ctgtttcatt taaagtaatg tcagccaaac gttcagcatg aagccctaac attttgattt
     1141 ggatgtattg gtcaacgaga gaagctgttc tgctttcaaa ttgcccttta taagccaaaa
     1201 aagccatcag aacaccgact gtaaatgaac catctaaaat tgcgcttgca ccaagataaa
     1261 ttatgataat attttccatg ctaaacaaca gtttatttga aaattcaaat aaagcagaga
     1321 gtttatctgt tgtcagcttg gtattgactg tattcacaaa taggctcatc caagtgccat
     1381 gtctttgata atgtttatca aataatttaa ctgattggat accacgaatg gtttccatga
     1441 aatatgagtt ttgtttggct tcatgaacaa tattttcttc tgttgcattt cttaatgggt
     1501 aatatgcaag ccaacgaatt agtatgtaca aaacaagtgt taaaagaaca atcagcgata
     1561 attgagtgct gtaaattgtc attaacacga aagtaaaaac agccattaag ctatttaaaa
     1621 ctaaaacaaa aaaagtagaa gttagtgttt cttggatatg atctattgaa ccaaatcttg
     1681 aaatcacatc tcctaaatgt cgtttactga aatagtcatt aggtaagtca agtaaccttt
     1741 taaatatatt ggctttccat tgtaaattaa gagttgtaga taggtgcata cctacccatg
     1801 cttgtaacag gctaattaac tgttgcagga tagccagtaa accaaatccc aaagtaaggg
     1861 tcagtaataa atttttatca gcagttacaa tgacatggtc tattacccat tgcataaaga
     1921 atggactaac caatgcaaag acttccaaag aaatagctaa tataagcatt tgaattaaag
     1981 agcgttttaa gcctgaaatc ccccttaata gagataatat tttgattttc tttgtttctt
     2041 ttttctcttc aaaatgggta ttggggaata attctagggc aatccctgtg aatttttgtg
     2101 aaacttcgtc cattttgatt tttcgcatac cgacagcagg gtccataatg acgatactgt
     2161 ctttggaaat ggaacaaagt acaacaaaat ggtttaagtt ccaatggaga atgcagggta
     2221 gttgtaaatt tgacagctca tctaactcta aacgcaaagc tcgtggcgtt aaattcattt
     2281 cattgccaaa tctcatgatg tctgcaagat ttgcgccctt taatgacagg gtgtattttt
     2341 ggcgtaacgt tcttaaatca gtgtgaaagc cataatagga caagatggat gtcaggcatg
     2401 ctaaaccaca ttcagcaact tctgtttgca gaatgacagg tagctttttg ttaaatccaa
     2461 aggacagtct tgataaataa tccattttta attgattttt cctgaaatgc tgtaaagtgg
     2521 gtcaagtacc cattcgtaca atttttttcg ttcatggaga atatctgctt ctaaaatcat
     2581 gccaatttga agcggcttgt tttctccgta tgctttaatc gtttgttttt ccaatttaac
     2641 tttcacaaga taggcaggtt catttaataa ggttgggtta gtgaaaatga tacctaaacc
     2701 tgatagctct tgtttaccga gagcagttct ggcaactgaa ataatttctc ctgtggcatg
     2761 tccaaatttt tggtaagggt acgcttggta acgtaaaaca actttatctt tcggtttaat
     2821 aaaaccaaca gctttactgg gtatgtaaag attggcgacc aattcagttt gttcagggac
     2881 aatgcttaac agcaatttag acggttcaac ttgttgccct atatcaacat taattgttga
     2941 tatataacct gatttactag ctcgtatggt ttgttcggat ttcaaatcaa aatccaaaat
     3001 ttcttggttc atttccgtaa tcgcacggtt gagttggctc aattcggttt tatggcgttc
     3061 aggcaggctg cttaatgtaa ttttctgttc atcaagttcc ctgattgcat tattttgttc
     3121 aagttttagg ctctccaaac gtgagcgttg ttccaataaa tggctttcgg cggtcatctt
     3181 atcttgttgg gatactgcgc cttgactggc taaaaacttg ttcttgttaa gggttttttc
     3241 cgctaaacga atttgacgat tttgccctgt aatttgctgt ttaatattct ctaattgatt
     3301 gtttaaacga tgaatgttgt tatgaacatt tttttgctca ttttgatgaa tgcgctttaa
     3361 acgttccaat tcttgtaatg ccaaagtttt tttaaggttg gcttctgctg ccaatttggc
     3421 ttgtacgttt cctttttcgc caaaacgcga tgtggaaagt ttgaacaatg gttcgccagc
     3481 tttgacaaag ttaccatctt caacaaattt atgcgtaatc gtgccgatat cggaagagta
     3541 aacacgaacc acccccatag ttggaagtaa ttgaccttca acggttgttt tattggtata
     3601 gctaccaaaa atcaaaaaga taatgataca cagagcaatg agaaaagcgc aaaaagtcag
     3661 aaataaaaaa gagaatggac gggtcaagat aacctgacct gtccacttat tttgttgggc
     3721 tacaaagact tcttttcgga ataagtggga catttattat tatttcttaa aaaaatggat
     3781 agaaaaacac caataattaa atacttatgc ctaataacat aaatttgcta ttgattgatg
     3841 tttctatttt aattacatta aatttaacca ataatccaga aatggttgca gctaaaatgc
     3901 aaaagacagt ggcgtaaatg gtttcagtat tcataatttt tctctttcaa aattgttcaa
     3961 tctaaataga aaaaggtaac ttcaatacat attgctgtaa ctgaaattac cttttcctta
     4021 atgatgtaat tatcttatgg cttcatcatg ccacgaccga tattgtatcc gccagcagca
     4081 gagaaaatac cagctccaat tgcgccaacc cagcctactg gtcctgtcca cttgttccgt
     4141 tgagctacaa agacttctgg tctgaaaaat gagtgattgt tagacatgtg tacttattta
     4201 tgtttctttg attgggataa atttgtaaaa acaaaaaaaa ggcaagttgt tattatagaa
     4261 gaacttaaaa tgcttaacat actcattgag gtttgtacca cataatgaga tacgagataa
     4321 tttgttggga gtaaaatcaa aaaaagatta aatatttaaa ataatcacta tattttttca
     4381 ttatttgttc ctttttgtat ttaagttctt atgaaaaact agggtatttt gaatagaaaa
     4441 tatcaaaata ccctagtttt attaaggaat attaccacca acaagtgctt ccatattgta
     4501 cagcaccagt gattgcacca ccaatagctc cgaatttcgc acttggaata gcaccaatac
     4561 cacctgcaaa tgaaccaaca attgccccac cagctgctcc acctaatgca ctaccaatgg
     4621 tattttttga gaaatcacgc cagttacaag cagcaccaga aacttgttaa atttcattca
     4681 aagtcaaaac ttgcataata ttcacctttg ttaagtgtgg ttgagaaaaa agtaaatttt
     4741 catcgttact cattatttgc aatcatgcat atactgtaac caagcactat attcagttgg
     4801 atcatagttt ttcctactgg gttgtctgat atgactattg gcactattgg cacaattggg
     4861 attagcaact ttattgtgag tacctaatgg attgatattt cctgtaaagt ggctattgca
     4921 tgcgcttaat gagcatagat ttattaatgt cagaaaggat aaaacaaaaa atcttaccat
     4981 tttttactaa taccaaaagt aggtttcgaa aactgtacac caagaccttg tacatatcca
     5041 aattgcttgc taacagaaaa ctctactcca ctattgttag gggaatagga agcaccaatt
     5101 ttaccaacag caaccatatc ttttttacct aggttgccgc caccctgtgc aaagatatgg
     5161 aatttgccac ctgacacttc aactaattca gaggtgtgta actctttcat gattttctcc
     5221 tgtaaagaag tatggttgga aatgtaaatc agaattaacc tttcttaagt ttaatttaat
     5281 actttttttg caagtatttt attttaaatt aaatcaatat tttaaaaata tttacttgta
     5341 tactgaatat ttacttgtat actgaaaaag aaactcctaa tggtggagtg gggcttttgt
     5401 ttaagtaagc attgaaaaaa tttaaatttc ccattatgat taacttaatg caagagcttt
     5461 ttcaggacgt acaaaccagc gaacgggcgt tctgatgtgg gcaggttggt tgtcgctaac
     5521 caatttcagt agctcaatcg tcagattggt atccggccct atcaagctaa ggagaggctg
     5581 cggctactac acaaagcacc tgaaattatt gggtgctttt gtctttttat ggcattgtac
     5641 caatttgtgt accacgaggg cttgttcgac caaacgcggt aacaactgaa tacacacttt
     5701 ccccctcaac ttctttgcgt tctaaaacga ctgtcccaat ccgagaatag acaatcatag
     5761 gacgatgatt gccccttaca tcactaaatt cactaaaaat ttttgcgcct gattgaaaaa
     5821 tcatttccac ataaaagggt tacacctgtt tcatcgtggc ttaatccaaa ccgcgttaaa
     5881 tctgctttgt gttctgccaa aatgtgaatc acaccaaagc cgtgattaac ctttttgtgt
     5941 tcgccgtgtt ttaaaacaat ttgacctgcc gggaagtctg ctctattgtt cggacaatag
     6001 ggggaatata ccaattactt taactacatc attttttgac atttttcttg cctttcaatt
     6061 taaaatccaa aatcatactg ccataattta gcaatccaaa aaaatttagg cagcagtcat
     6121 ggtttcgtat tttagaaaac gaaacttggt ttggtgtttt gaagcatttc agtacacaaa
     6181 catggaattt taaattgtct tgtgttgcct tgatttagga tgttccagtt ttttaataaa
     6241 tagtttgggg ctgtcctaga taactaggac aaacttgatt ttactaattg ttttaaaatg
     6301 gaacaagaac ttttatctca ctgttgttaa aacgtcgttc gcactccttt aaatacagct
     6361 caaaatgcgc tttgggaatg ccgttaaact tg
//
LOCUS       DAXHPG010000121         6382 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXHPG010000121 DAXHPG010000000
VERSION     DAXHPG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.121"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            1..2004
                     /locus_tag="ACFM6F_001640"
                     /pseudo
     CDS             1..2004
                     /locus_tag="ACFM6F_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1923..2216)
                     /locus_tag="ACFM6F_001641"
     CDS             complement(1923..2216)
                     /locus_tag="ACFM6F_001641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165024.1"
                     /translation="MPLFQTLPPDAATMPSEARIGFQTAAKNRRFSAGFIIMPRPSGQ
                     QGSDWFAGRRAVPCRCAACTSKHFPTHQNSHLSDNAASLSPSVLLPYPMRADI"
     gene            2246..3712
                     /locus_tag="ACFM6F_001642"
     CDS             2246..3712
                     /locus_tag="ACFM6F_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6165025.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKSPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFEDRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(3913..4881)
                     /locus_tag="ACFM6F_001643"
     CDS             complement(3913..4881)
                     /locus_tag="ACFM6F_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165026.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDGTNGGAQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            4959..6251
                     /locus_tag="ACFM6F_001644"
     CDS             4959..6251
                     /locus_tag="ACFM6F_001644"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC6165027.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGKVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
BASE COUNT         1487 a         1614 c         1929 g         1352 t
ORIGIN      
        1 gtcgggttaa tctattaaca ttatctgttt tatcgctgtt ttgcacgcca tatgtttgag
       61 gttcggatgc gtacgatccc gtcaaagaag ccgagattaa aaacaaattt attttagaag
      121 cggcggaaga cagaaattcc cacgtttggc gcggcccgtg cagtgtgtct tttgattgct
      181 tcggtgcttt cagttatcag cccggttcgg atactcgttc tacaaatatt ggcgatgctg
      241 ccgatttttc attttcagac aagccggcaa acggcgtttc ccattatttt tccagcggta
      301 aaaccgatcc aaattcatct gaagccttgt atgacgaaat caatatcacg ggtaaaaact
      361 acaatagcgg catcctcgcc gtcgataata tgcccgttgt taagaaatat gttacagaga
      421 attacggagc tgatttaaag caggcggtta aggatcaatt aaaggattta tataaaacaa
      481 gacccgaaga ttggggaaaa aaataaaaaa atagttgagg aagcgataat aaaacaatat
      541 ggaataccta gaaataaaat taataaattt agaaaagtta ttgatccaat tatagaagaa
      601 gctgtactca ctcctcatag taatacaaca ccgaccaatc tcgacaacat cttcaataaa
      661 aaattacacg tcaaaatcga aaacaaatcc cacgtcgctg gacaggtgtt ggaactgacc
      721 aagatgacgc tgaaagattc cctttgggaa ccgcgccgcc attccgacat ccatacgctg
      781 gaaacttccg ataatgcccg catccgcctg aacacgaaag atgaaaaact gaccgtccat
      841 aaggattatg cgggcggcgc ggatttcctg ttcggctacg acgtgcggga gtcggacgaa
      901 cccgccctga cctttgaaga caaagtcagc ggacaatccg gcgtggtttt ggaacgccgg
      961 ccggaaaatc tgaaaacgct cgacgggcgc aaactgattg cggcaaaaac ggcggattcc
     1021 ggttcgtttg cgtttaaaca aaattaccgg cagggactgt acgaattatt gctcaagcaa
     1081 tgcgaaggcg gattttgctt gggcgtgcag cgtttggcta tccccgaggc ggaagcggtt
     1141 ttatatgccc aacaggctta tgcggcaaat actttgtttg ggctgcgtgc cgccgacagg
     1201 ggcgacgacg tgtatgccgc cgatccgtcc cgtcaaaata tacggggtgg cgcggctgcg
     1261 gacgggcggc gcaaaggcgt gcaaatcggc ggcgaggtgt ttgtacggca aaatgaaggc
     1321 agccggctgg caatcggcgt gatgggcggc agggtcggcc agcacgcatc agttaacagc
     1381 aaaggcggtg cggcaggcag tgatttgcat ggttatggcg ggggtgttta tgctgcgtgg
     1441 catcagttgc gcgataaaca aacgggtgcg tatttggacg gctggttgca ataccaacgt
     1501 ttcaaacacc gcatcaatga tgaaaaccgt gcggaacgct acaaaaccaa aggttggacg
     1561 gcttctgtcg aaggcggcta caacacgctt gtggcggaag gcgttgtcgg aaaaggcaat
     1621 aatgtgcggt tttacctgca accgcaggcg cagtttacct acttgggcgt aaacggcggc
     1681 tttaccgaca gcgaggggac gggggtcgga ctgctcggca gcggtcagtg gcaaagccgc
     1741 gccggcattc gggcaaaaaa ccgttttgct ttgcgtaatg gtgtcaatct tcagcctttt
     1801 gccgctttta atgttttgca caggtcaaaa tctttcggcg tggaaatgga cggcgaaaaa
     1861 cagacgctgg caggcaggac ggcgctcgaa gggcggttcg gcattgaagc cggttggaaa
     1921 ggtcatatgt ccgcacgcat cggatacggc aaaaggacgg acggcgacaa agaagccgca
     1981 ttgtcgctca aatggctgtt ttgatgcgtc gggaaatgtt ttgacgtaca ggcggcacac
     2041 cggcacggca ccgcacgccg ccccgcaaac caatccgaac cctgctgccc cgaagggcgg
     2101 ggcataatga tgaaaccggc ggaaaaccgc cggttttttg ccgccgtttg aaacccgatt
     2161 ctggcttcag acggcattgt cgcggcatcg ggcggcaggg tttggaacag cggcataaaa
     2221 aactgataca atccgccgat tgataatggt tattttttat ttttgtggga agacatttat
     2281 gcctgcacga aacagatgga tgctgctgct gcctttattg gcaagtgcgg catatgccga
     2341 agaaacaccg cgcgaaccgg atttgagaag ccgtcccgag ttcaggcttc atgaagcgga
     2401 ggtcaaacca atcgacaggg agaaggtacc ggggcaggtg cgggaaaaag gaaaagtttt
     2461 gcagattgac ggagaaaccc tgctgaaaag tcccgaattg ttgtcccgcg cgatgtattc
     2521 cgcagtggtc tcaaacaata ttgccggtat ccgcgttatt ttgccgattt acctacaaca
     2581 ggcgcagcag gataagatgt tggcacttta tgcacaaggg attttggcgc aggcagacgg
     2641 tagggtgaag gaggcgattt cccattaccg ggaattgatt gccgcccaac ccgacgcgcc
     2701 cgccgtccgt atgcgtttgg cggcagcatt gtttgaagac aggcagaacg aggcggcggc
     2761 agaccagttc gaccgcctga aggcggaaaa cctgccgccg cagctgatgg agcaggtcga
     2821 gctgtaccgc aaggcattgc gcgaacgcga tgcgtggaag gtaaacggcg gcttcagcgt
     2881 tacccgcgaa cacaatatca accaagcccc gaaacggcag cagtacggca aatggacttt
     2941 cccgaaacag gtggacggca cggcggtcaa ttaccggctc ggcgcggaga aaaaatggtc
     3001 gctgaaaaac ggctggtaca cgacggcggg cggcgacgtg tccggcaggg tttatccggg
     3061 gaataagaaa ttcaacgata tgacggcagg cgtttccggc ggcatcggtt ttgccgaccg
     3121 gcgcaaagat gccgggctgg cagtgttcca cgaacgccgc acctacggca acgacgctta
     3181 ttcttacacc aacggcgcac gcctttattt caaccgttgg caaaccccga aatggcaaac
     3241 gttgtcttcg gcggagtggg ggcgtttgaa gaatacgcgc cgggcgcgtt ccgacaatac
     3301 ccatttgcaa atttccaatt cgctggtgtt ttaccggaat gcgcgccaat attggatggg
     3361 cggtttggat ttttaccgcg agcgcaaccc cgccgaccgg ggcgacaatt tcaaccgtta
     3421 cggcctgcgc tttgcctggg ggcaggaatg gggcggcagc ggcctgtctt cgctgttgcg
     3481 cctcggcgcg gcgaaacggc attatgaaaa acccggcttt ttcagcggtt ttaaagggga
     3541 aaggcgcagg gataaagaat tgaacacatc cttgagcctt tggcaccggg cattgcattt
     3601 caaaggcatc acgccgcgcc tgacgctgtc gcaccgcgaa acgtggagta acgatgtgtt
     3661 caacgaatat gagaaaaatc gggcgtttgt cgagtttaat aaaacgttct gattgctgtt
     3721 ccttttcgga ggaaaccctg ccggcggcgg tatcacggcg ggcatcggcg gctttcgggc
     3781 ggtgctttgc gtgccgccgc gtgtgcggaa acgcattccg gtttttccgg cataacggcg
     3841 atgcgaggta aaatgccgtc tgaaacccga ttcgggcttc agacggcatt gtcgcggttg
     3901 cggcaggcgg gttcaccaga ttccgtcaaa ggttttcgcg ccgcgccaaa atttccacct
     3961 gtcggcgggt ttgaaggtca gcgtaccgcc gtgttgtccg tccgtggtga tgtccagccg
     4021 tttgattttg ccggtacgga cggcttcgta aagcggtgcg aaccagcgtt cttcccactg
     4081 ctgcaatatt gccgcatacc gctccctgtc ccctgtcagg gcggtcaggc gcaaatcgtc
     4141 cataaacagg atatggtgcg tgtcaggcag gtgtgccgcc gtttcttcat aggcgcggaa
     4201 gttgtcgggc aatgcgcggc ggtcggggtg gaaacggctc caaaccgtat cggcgaaaag
     4261 tgtgccgcct tgcgcgccgc cgtttgtgcc gtcccaaagc cataagccgt tcaactcggg
     4321 cagcccgcgt tttttgcggt tatggttgac ggggtgcgcc gccagccaca tttggatttc
     4381 ggtttggacg cgcagccatt ccaacgcatc ttctccgtcc ggctgatcgt ccgcgcccaa
     4441 caatccgccc aagtccaaaa cgggcttcgc gccccagcgg tacgcgcaag gaagggaaac
     4501 cagccataat tcgggcagga cgggaacgaa acgccatgga atgtcgccgt aaaacgccga
     4561 caggtcgcgg cagagccgtt ccgcttcatc cgtaccgacg ttcagatatt ccgccgttag
     4621 cacatttgcc tgatgcatcc ccatcttttg ccagacgggc gtggcgagcg cgacggcttc
     4681 agacggcata ttcaggcttt gcgccgcgcg ttccgccagt ctgccgcacc acaaataacg
     4741 cgcgtaaaat gccgaagccg tgcagctttg gcggtgcagc gagccgtatt gcaggatttt
     4801 gttgaaggcg ggcaggcata gaggtattcg gatttcgtct tcatccaaat tgagcgaggg
     4861 gagggcgagg gtgagtttca tcgtttgacg tttcagaaat gcaggtcagg cgcaacatta
     4921 tagaggattc ggcgcaaacg ccgtcaaaaa ggaacaatat ggctgtcttc ccactttcgg
     4981 caaaacatcg gaaatacgcg ctgcgtgcgc ttgccgtttc gattattttg gtgtcggcgg
     5041 catacattgc ttcgacagag aggacggagc gcgtcagacc gcagcgcgtg gaacaaaatc
     5101 tgccgccgct gtcttggggc ggcagcggcg ttcagacggc atattgggtg caggaggcgg
     5161 tgcagccggg cgactcgctg gcggacgtgc tggcgcgttc gggtatggcg cgggacgaga
     5221 ttgcccgaat cacggaaaaa tatggcggcg aagccgattt gcggcatttg cgtgccgacc
     5281 agtcggttca tgttttggtc ggcggcgacg gcggcgcgcg cgaagtgcag ttttttaccg
     5341 acgaagacgg cgagcgcaat ctggtcgctt tggaaaagaa aggcggcata tggcggcggt
     5401 cggcttctga ggcggatatg aaggttttgc cgacgctgcg ttcggtcgtg gtcaaaacgt
     5461 cggcgcgcgg ttcgctggcg cgggcggaag tgcccgtcga aatccgcgaa tccttaagcg
     5521 ggattttcgc cggccgcttc agccttgacg gtttgaagga aggcgatgcc gtgcgcctga
     5581 tgtacgacag cctgtatttc cacgggcagc aggtggcggc gggcgatatt ttggcggctg
     5641 aagtcgttaa gggcggcaca aggcatcagg cgttctatta ccgttcggac aaggaaggcg
     5701 gagggggcgg caattattat gatgaagacg gcaaggtgtt gcaggaaaaa ggcggcttca
     5761 acatcgagcc gctggtctat acgcgcattt cttcgccgtt cggctaccgt atgcacccca
     5821 tcctgcacac atggcggctg cacacgggca tcgattatgc cgcaccgcag ggaacgccgg
     5881 tcagggcttc cgccgacggc gtgattacct ttaaaggccg gaagggcgga tacggcaacg
     5941 cggtgatgat acgccacgcc aacggtgtgg aaacgctgta cgcgcacttg agcgcgtttt
     6001 cgcaggcaca aggcaatgtg cgcggcggcg aggtcatcgg ttttgtcggt tcgacagggc
     6061 gttcgaccgg gccgcacctg cattacgagg cacgcatcaa cgggcagccc gtcaatcctg
     6121 tttcggtcgc attgccgaca cccgaattga cgcaggcgga caaggcggcg tttgccgcgc
     6181 agaaacagaa ggcggacgcg ctgcttgcgc gcttgcgcgg cataccggtt accgtgtcgc
     6241 aatcggattg aagtttgaac cggcgacgaa aacaatgccg tctgaaaatc tgcaaacagg
     6301 ttttcagacg gcatttatag tggattaaca aaaaccagta cgtcgttgcc tcgccttagc
     6361 tcaaagagaa cgattctcta ag
//
LOCUS       DAXHPG010000122         6308 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXHPG010000122 DAXHPG010000000
VERSION     DAXHPG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.122"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            253..2385
                     /locus_tag="ACFM6F_001645"
     CDS             253..2385
                     /locus_tag="ACFM6F_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6165028.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGGSFDLDSVDTEAPRPAPK
                     YQDVSSEKPQAQKDQGGYGFAMRLKRRNQHPYAEPKEDEIKLENDNWERTRLPVNPQN
                     LPQKQESIIQAVKTDDDNNIYTSPYLTQSSHNSHNGSTNGGANQPKNEATGYKNFKYV
                     YSGWFYKYAASEREFSKKNFKSGDDGYIFYHGKEPSRQLPASGNITYKGVWHFVTDTK
                     KGQKFNDILGTSKGQGNKYSGFSGDDDEQYSNKNETTLQSDHEGYGFTSNLEVDFGSK
                     KLTGKLIRNNRVTNAIPNDKYTTQYYSLDAQITGNRFNGTATATDKKENETKLHPFVS
                     DSSSLSGGFFGPQGEELGFRFLSNDQKVAVVGSAKTKDKPENGAAALGGAGAAASDGA
                     AGTSSENGKLTTVLDAVELKSGGKEVKNLDNFSNAAQLVVDGIMIPLLPETSESGNNQ
                     ANQGKNGGTAFTRKFDHTPESDKKDTQAGTPMNGAQTASNAAGDTNGKTKTYAVEVCC
                     SNLNYLKYGMLTRKTAGNTGEGGNGSPTAAQTAQGAQSMFLQGERTDEKEIPNDQNVV
                     YRGSWYGHIANDTSWSGNASDREGGNRADFTVNFGTKKINGTLTAENRQEATFTIDGK
                     IEGNGFSGTAKTAELGFALDQSNNTRTPKAYITDAKVQGGFYGPKAEELGGWFAYPGD
                     KQTKNATNASGNSSATVVFGAKRQQPVR"
     gene            2472..5216
                     /gene="tbpA"
                     /locus_tag="ACFM6F_001646"
     CDS             2472..5216
                     /gene="tbpA"
                     /locus_tag="ACFM6F_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HFC6165029.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDTHPYANFIVEEECESGNYETCKSKP
                     KKDIVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDANKKQAGSLSGNGKYAGNHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWVDYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFDS
                     NLRHQDYYYQHANRAYSLKTPPKNGNQNSPNGDKSKPYWVSIGGGNVVTGQICLFGNN
                     TYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLS
                     WNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGDKIKAVKIDPEKSFNKEVGIV
                     FKGDFGNLEASWFNNAYRDLIVRGYEAQIKNGKEEAKGDPAYLNAQSARITGINVLGK
                     IDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQ
                     PEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIK
                     KHFTLRAGVYNLLNHRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF
                     "
     gene            5234..5389
                     /locus_tag="ACFM6F_001647"
     CDS             5234..5389
                     /locus_tag="ACFM6F_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165030.1"
                     /translation="MPSERLQTAFFTQFPPFPIIPDNTVILKPVIPTQAGIQSVRFRF
                     FEVSGNF"
BASE COUNT         1812 a         1570 c         1574 g         1352 t
ORIGIN      
        1 ggagcggttt ctgctttttc caataaatga ccccaactta aaatcccgtc attcccgcgc
       61 aggcgggaat ctagacattc aatgctaagg caatttctcg gaaatgactg aaactcaaaa
      121 aactggattc ccgcctgcgc gggaatgacg ggattttggt gtattcccta aaaaaataaa
      181 aaagtattta caaatttgtt aaaaataaat aaaataataa tctttatcat tctttaattg
      241 aattaggttt atatgaacaa tccattggtg aatcaggctg ctatggtgct gccggtgttt
      301 ttgttgagtg cttgtctggg cggaggcggc ggcagtttcg atcttgattc tgtcgatacc
      361 gaagccccgc gtcccgcgcc aaagtatcaa gatgtttctt ccgaaaaacc gcaagcccaa
      421 aaagaccaag gcggatacgg ttttgcgatg aggttgaaac ggaggaatca gcatccttat
      481 gcggaaccta aagaagatga aattaaatta gaaaatgata attgggagag aacgagattg
      541 ccagttaatc cccaaaatct accccaaaaa caagaatcaa ttattcaggc agtaaaaaca
      601 gacgatgaca acaatattta cacttcccct tatctcacgc aatcaagcca taattctcac
      661 aacggcagca ccaatggcgg tgcaaaccaa ccaaaaaacg aagcaacagg ttacaaaaat
      721 ttcaaatatg tttattccgg ttggttttat aaatatgcgg cgagtgaaag agaattcagt
      781 aaaaaaaatt ttaagtcagg cgacgacggt tatatcttct atcacggtaa agaaccttcc
      841 cgacaacttc ctgcttctgg aaacattacc tataaaggcg tatggcattt tgtaaccgat
      901 acgaaaaagg gacaaaaatt taacgatatt cttggaacct caaaaggaca aggaaataag
      961 tacagcggat tttcgggcga tgatgatgaa caatattcta ataaaaatga aacgacttta
     1021 caaagcgatc atgaaggtta tggttttacc tcaaatttag aagtggattt cggcagtaaa
     1081 aaattgacgg gtaaattaat acgcaataat agagttacaa acgctattcc taacgataaa
     1141 tacaccaccc aatactacag ccttgatgca caaataacag gcaaccgctt caacggcacg
     1201 gcaacggcaa ctgacaaaaa agagaatgaa accaaactac atccctttgt ttccgactcg
     1261 tcttctttga gcggcggctt tttcggcccg caaggtgagg aattgggttt ccgctttttg
     1321 agcaacgatc aaaaagttgc cgttgtcggc agcgcgaaaa ccaaagacaa accggaaaat
     1381 ggcgcagcgg ctctaggcgg tgcaggtgcg gcagcatcgg acggtgcggc aggcacgtcg
     1441 tctgaaaacg gtaagctgac cacggttttg gatgcggtcg agctgaaatc gggcggtaag
     1501 gaagtcaaaa atctcgacaa cttcagcaat gccgcccaac tggttgtcga cggcattatg
     1561 attccgctcc tgcccgagac ttccgaaagt gggaacaatc aagccaatca aggtaaaaac
     1621 ggcggaacag cctttacccg caaatttgac cacacgccgg aaagtgataa aaaagacacc
     1681 caagcaggta cgccgatgaa tggggcgcaa accgcttcaa atgcggcagg tgataccaat
     1741 ggcaaaacaa aaacctatgc agtcgaagtc tgctgttcca acctcaatta tttgaaatac
     1801 ggaatgttga cgcgcaaaac tgccggcaac acgggggaag gcggcaacgg cagcccaacc
     1861 gccgcgcaaa cggcacaggg cgcacaaagt atgttcctcc aaggcgagcg taccgatgaa
     1921 aaagagattc caaacgacca aaacgtcgtt tatcgggggt cttggtacgg gcatattgcc
     1981 aacgacacaa gctggagcgg caatgcttca gatagagagg gcggcaacag ggcggacttt
     2041 accgtgaatt ttggtacgaa aaaaattaac ggaacgttaa ccgctgaaaa caggcaggag
     2101 gcaaccttta ccattgatgg taagattgag ggcaacggtt tttccggtac ggcaaaaact
     2161 gctgaattag gttttgctct cgatcaaagc aataacaccc gcacgcctaa ggcatatatc
     2221 acagatgcca aggtgcaggg cggtttttac gggcctaaag ccgaagagtt gggcggatgg
     2281 tttgcctatc cgggcgataa acaaacgaaa aatgcaacaa atgcatccgg caatagcagt
     2341 gcaactgtcg tattcggtgc gaaacgccaa cagcctgtgc gataagcacg gctgccgaac
     2401 aatcaagaat aaggcctcag acggcaccgc tccttccgat gccgtctgaa agcgaagatt
     2461 agggaaacac tatgcaacag caacatttgt tccgattaaa tattttatgc ctgtctttaa
     2521 tgactgcgct gcccgtttat gcagaaaatg tgcaagccgg acaagcacag gaaaaacagt
     2581 tggataccat acaggtaaaa gccaaaaaac agaaaacccg ccgcgataac gaagtaaccg
     2641 gtttgggcaa attggtcaaa accgccgaca ccctcagcaa ggaacaggta ctcgatatcc
     2701 gcgacctgac gcgttacgac cccggcatcg ccgtcgtcga acaggggcgc ggcgcaagct
     2761 cgggctactc gatacgcggt atggacaaaa accgcgtttc cttgacggtg gacggcttgg
     2821 cgcaaataca gtcctacacc gcgcaggcgg cattgggcgg gacgaggacg gcgggcagca
     2881 gcggcgcaat caatgaaatc gagtatgaaa acgtcaaagc tgtcgaaatc agcaaaggct
     2941 caaactcggt cgaacaaggc agcggcgcat tggcgggctc ggtcgcattt caaaccaaaa
     3001 ccgccgacga tgttatcggg gaaggcaggc agtggggcat tcagagtaaa accgcctatt
     3061 ccggcaaaaa ccgggggctt acccaatcca tcgcgctggc ggggcgcatc ggcggtgcgg
     3121 aggctttgct gatccacacg ggtcggcgcg cgggggaaat ccgcgcccac gaagatgcag
     3181 gacgcggcgt tcagagcttt aacaggctgg tgccggttga ggatacccat ccgtacgcaa
     3241 atttcatcgt tgaagaagaa tgcgaaagcg ggaattacga aacgtgtaaa agcaaaccga
     3301 aaaaagatat tgtcggcaaa gacgaacggc aaacggtttc tacccgagac tacacgggtc
     3361 ccaaccgctt cctcgccgat ccgctttcat acgaaagccg gtcgtggctg ttccgcccgg
     3421 gttttcgttt tgaaaacaaa cggcactaca tcggcggcat actcgaacac acgcaacaaa
     3481 ctttcgacac gcgcgatatg acggttccgg cattcctgac caaggcggtt tttgatgcaa
     3541 ataaaaaaca ggcgggttct ttgtccggca acggcaaata cgcgggcaac cacaaatacg
     3601 gcggactgtt taccaacggc gaaaacggtg cgctggtggg cgcggaatac ggtacgggcg
     3661 tgttttacga cgagacgcac accaaaagcc gctacggttt ggaatatgtc tataccaatg
     3721 ccgataaaga cacttgggtg gattatgccc gcctctctta cgaccggcag ggcatcggtt
     3781 tggacaatca ttttcagcag acgcactgtt ctgccgacgg ttcggacaaa tattgccgcc
     3841 cgagtgccga caagccgttt tcctactaca aatccgaccg cgtgatttac ggggaaagcc
     3901 acaggctctt gcaggcggca ttcaaaaaat ccttcgatac cgccaaaatc cgccacaacc
     3961 tgagcgtgaa tctcggtttt gaccgctttg actctaatct ccgccatcag gattattatt
     4021 atcaacatgc caaccgcgcc tattcgttga aaacgccccc taaaaacggc aatcaaaaca
     4081 gccccaacgg cgacaagagc aaaccctatt gggtcagcat aggcggggga aatgtggtta
     4141 cggggcaaat ctgcctcttt ggcaacaata cttatacgga ctgcacgccg cgcagcatca
     4201 acggcaaaag ctattacgcg gcagttcggg acaatgtccg tttgggcagg tgggcggatg
     4261 tcggcgcggg gttgcgctac gactaccgca gcacgcattc ggacgacggc agcgtttcca
     4321 ccggcacgca ccgtaccctg tcctggaacg ccggcatcgt cctcaaacct gccgactggc
     4381 tggatttgac ttaccgcact tcaaccggct tccgcctgcc ttcgtttgcg gaaatgtacg
     4441 gctggcggtc gggcgataaa ataaaagccg tcaaaatcga tccggaaaaa tcgttcaaca
     4501 aagaagtcgg catcgtgttt aaaggcgatt tcggcaactt ggaggcaagt tggttcaaca
     4561 atgcctaccg cgatttgatt gtccggggtt atgaagcgca aattaaaaac ggcaaagaag
     4621 aagccaaagg cgacccggct tacctcaatg cccaaagcgc gcggattacc ggcatcaatg
     4681 ttttgggcaa aatcgattgg aacggcgtat gggataaatt gcccgaaggt tggtattcta
     4741 catttgccta taatcgtgtc cgtgtccgcg acatcaaaaa acgcgcagac cgcaccgata
     4801 ttcaatcaca tctgtttgat gccatccaac cttcgcgcta tgtcgtcggc ttgggctatg
     4861 accaaccgga aggcaaatgg ggtgtgaacg gtatgctgac ttattccaaa gccaaggaaa
     4921 tcacagagtt gttgggcagc cgggctttgc tcaacggcaa cagccgcaat acaaaagcca
     4981 ccgcgcgccg tacccgccct tggtatattg tggatgtgtc cggttattat acgattaaaa
     5041 aacactttac cctccgtgcg ggcgtgtaca acctcctcaa ccaccgctat gttacttggg
     5101 aaaatgtgcg gcaaactgcc ggcggcgcag tcaaccaaca caaaaatgtc ggcgtttaca
     5161 accgatatgc cgcccccggt cgcaactaca catttagctt ggaaatgaag ttttaaacgt
     5221 ccaaacgccg caaatgccgt ctgaaaggct tcagacggca ttttttacac aattcccacc
     5281 gtttcccatc atccccgaca ataccgtaat cttgaaaccc gtcattccca cgcaggcggg
     5341 aatccagtcc gttcggtttc ggttttttga ggtttcgggt aacttctaaa ccgtcattcc
     5401 cgcgaaggcg gaaaatcaaa aacagaaacc tcaaatcccg tcattcccgc gcaggcggga
     5461 atctagggcg taaaatctaa agaaaccgtt ttatccgata agtttccgca ccgacagact
     5521 agattcccgc ctgcgcggga atgacgttat atttttcgca tttgataaaa aagaccgttt
     5581 gaaatttttt cagcggacgc aaagtattgc gtaaaatgct gcttataaga aacgcaaggc
     5641 gggcgtagga tgtgcagcgt ggacataggg ctggctgtag ggtgggcttc agcccaccaa
     5701 tcccaccgtt tccacctatt cccccaactc cgtcaatgtt atccattccg cccattccca
     5761 ccgaaaaccg aaaccgccgt attcccaaaa acctttgatg cggtgaaatt ggtgggctga
     5821 agcccaccct acagcccacc ctacggctcg ccgaaatttc gtcattcccg cgcaggcggg
     5881 aatccaggtc tgtcggtgcg gaaacttatc ggataaaacg gtttcttgag attttacgtc
     5941 ctagattccc acttccgtgg gaatgacggg atgcaggttt tcgtgcggac gcgttcggat
     6001 tcacgactgc gcgggaatga cgggatgcag gttttcgtgc ggacgcgttc ggattcacga
     6061 ctgcgcggga atgacgggat ttcagtttgc ggattgattt gaagttgcaa aatcccaacg
     6121 gatcggatta ccgctttcgc gtttcaaagt tacggcgtta tcggaaaaac agaaaatcaa
     6181 agctgcaaga atttatttaa aacaaccgaa tttcaacgga tcggattctc gcctgtaggg
     6241 aatgacggcg gaaggttttt tgtcttttct gacagatgtc cgcaatctga aatcctgacc
     6301 gtgggaac
//
LOCUS       DAXHPG010000123         6255 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXHPG010000123 DAXHPG010000000
VERSION     DAXHPG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.123"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..139)
                     /locus_tag="ACFM6F_001648"
     CDS             complement(<1..139)
                     /locus_tag="ACFM6F_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HFC6165031.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGISGAVALVEPLINTVVF
                     YS"
     gene            261..1214
                     /locus_tag="ACFM6F_001649"
     CDS             261..1214
                     /locus_tag="ACFM6F_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6165032.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAEAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPAVREGYVAFEDAVRTADVLSLHCPLNAQTENMIGEN
                     ELRQMKPCAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(1289..3346)
                     /gene="metG"
                     /locus_tag="ACFM6F_001650"
     CDS             complement(1289..3346)
                     /gene="metG"
                     /locus_tag="ACFM6F_001650"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC6165033.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKTGGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGVKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGCLKDVADSALLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTATN
                     AARFLNLDAITWTNTRVTLGKHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAVPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(3462..5300)
                     /gene="glmS"
                     /locus_tag="ACFM6F_001651"
     CDS             complement(3462..5300)
                     /gene="glmS"
                     /locus_tag="ACFM6F_001651"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC6165034.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGKLVEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLSYHVALARGTDVDKPRNLAKSVTVE"
     gene            5582..6100
                     /locus_tag="ACFM6F_001652"
     CDS             5582..6100
                     /locus_tag="ACFM6F_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165035.1"
                     /translation="MKQMLLAVGVVEVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
BASE COUNT         1336 a         1472 c         1901 g         1546 t
ORIGIN      
        1 cactatagaa aacgacggtg ttgattaggg gttcgaccag cgcgaccgcc ccggatatgc
       61 ctatactgcc cgtcagtaca taggttacac tgaaggcggt gctgaaatgc agtgcggcaa
      121 aagtcagggt tttaagcatc atcctctccc tgattggaca ttgacggagg aatgataaag
      181 attatcataa ggatgtgcgg tttaaatttg ctatttactg ttagtgtaaa taaatcaact
      241 ttcaaataag gatagaaata atgaatcaca aaaagatcgt tgttttggat gcggatactt
      301 tgcccggccg ggtttttcat tttgattttc cgcacgagct tgcggtttac ggtacgacag
      361 gtgcggatga aacggcagaa cgggtgcgcg atgcacatat tgtcattact aacaaagtga
      421 tgatttccgc cgatattatt gcggctaatc cgcagttgga gctgattgcc gtcagtgcga
      481 ccggcgtgaa caatgtcgat attggggcgg cggaggcggc gggcgttgcg gtatgcaatg
      541 tccgcgccta cggaaacgaa tcggttgcgg aacacgcctt tatgctgatg attgcgttaa
      601 tgcggaattt gcctgcctat cagcgtgatg ttgcggcagg attgtgggaa aagtcgccgt
      661 ttttctgcca ttacggcgct ccgattcggg atttgaacgg taaaacgctg gcggttttcg
      721 gacgcggcaa tatcggacgg acgctggccg gatatgcaca ggcgttcggt atgggggtgg
      781 tgtttgccga acacaaacac gcgcccgctg tgcgcgaagg ctatgttgcc tttgaagatg
      841 cggtacggac tgccgacgtg ttgtcgctgc actgtccgct gaacgcccaa actgaaaata
      901 tgataggcga aaacgaattg cggcagatga agccctgtgc ggttttgatt aattgcgggc
      961 gcggcgggct ggtggatgaa aacgcgctgc ttgccgcact caaatacggg cagatcggcg
     1021 gggcgggtgt cgatgttttg acggaggagc cgccaagggg cggcaatccc ctgctgaatg
     1081 cacgacttcc caatctgatt gttacgccgc ataccgcgtg ggcgagccgc gaggctttgg
     1141 acaggctgtt cgatatattg ttggcgaaca ttcacgcctt tgtgaaagga gaggcgcaaa
     1201 accgcgtggt ttgagcttgt cgggatgacg gaaaacaatg ccgtctgaac gcccaaaggg
     1261 ttcagatggc attttttgag atttctactt aaccgacttt gtcgcccggt tgcgcgcctg
     1321 catccacatc taagagcttc agtttgccgt ctgccgtggc ggcactcaaa atcatgcctt
     1381 cggatacgcc gaattttgcc attttgcgcg gggcgaagtt ggcgacggcg atgaccatgc
     1441 ggccgttcaa ttcggcaggg ttcgggtaag acgcggcgat gccggagaag ataatgcgtt
     1501 tttcaaaacc aaaatcgagg tcgaatttta agagtttggt gctgccttcg acggcttcgc
     1561 agttcaatac tttggcgacg cgcatatcga ttttcataaa gtcgtcgaaa ctcgcctgtt
     1621 cggcaacttt ttcgtatttg ctctcttcgg cggcaggcac ggctgcggcg gcgatgcttt
     1681 gtttgttggc ttcgattaaa tcgtccactt gtttttgctc cactcgttgc attaaatgtt
     1741 cgtatttgtt gatggcgtgt ttgcccaagg ttacccttgt gttcgtccaa gtaatcgcgt
     1801 ccagattgag gaaacgcgcg gcattggtgg cggtttgcgg caatacgggg gcgaggtagg
     1861 cggtcagcat cgtaaaggcg ttgatgagtt cgctgcatac ttcgtgcagg cgttcgtctt
     1921 ggccttcttg tttcgccagt tcccaaggtt tgttggcatc gacgtattcg ttgacaatgt
     1981 ctgccaaggc catgatgtcg cgcagggctt tggcgtattc gcggctttcg tagcattcgg
     2041 caatggcttc gctttgtgcg gtcagttttg ccagcaatgc gctgtcggca acgtctttca
     2101 gacagccttc aaagcgtttg gcgatgaaac ctgaggcgcg ggcggcgatg ttgacgtatt
     2161 tgccgacgag gtcgctgttg acgcggctga taaagtcttg caggttcaaa tcgatgtctt
     2221 cgattttgct gttgagtttg gcggcgatgt agtagcgcat ccactcgggg tttaaacctt
     2281 gttccagata ggatttggcg gtgataaacg tgccgcgcga tttggacatt ttttgtccgt
     2341 ctacggtcaa aaagccgtgt gcgtacacgc cggtcggggc gcggtggccg gagaaatgca
     2401 gcatggcagg ccagaacagg gcgtggaaat agaggatgtc tttgccgatg aagtggtaca
     2461 tctcggtttg gctgtcggct ttgaagtatt cgtcaaaatc gacgccgatg cggtcgcaca
     2521 ggtttttaaa cgacgccatg tagccgacgg gcgcgtccag ccagacgtag aagtatttgc
     2581 ccggcgcatc ggggatttca aaaccgaaat acggcgcgtc gcgggaaatg tcccagtcgg
     2641 acagggtggt ttcttcgcct tcgcccagcc attctttcat tttgttgagg gcttctggtt
     2701 gcagatgggg cttgccgtcg tgcgggttgt tgccggaagt ccaagctttg aggaagtcgg
     2761 cgcattcgcc cagtttgaag aagaagtgtt cggattcgcg caattcgggt ttcacgccgg
     2821 aaacggcgga atacgggttg atcagttcgg tcggggagta ggtcgtgccg cagacttcgc
     2881 agttgtcgcc gtattggtct tgagcgtggc atttggggca ttcgcctttg acgaagcggt
     2941 cgggcagaaa catttgtttt tcggggtcga aaagctgctc gatgacgcgg ctctcgattt
     3001 tgccgccggt tttcagcgcg cggtaaatat cttgggaaaa ctgtttgttt tcaggggaat
     3061 gggtgctgta ataattgtcg tagccgatgt tgaaaccggt aaagtcggcg aggtgctctt
     3121 cgcgcacttt ggcaatcatg tcttcagggg caatgccttg tttttgcgcg gcaagcatca
     3181 cgggcgtgcc gtgggtgtcg tcggcgcagc agtagtagca ttcgtggccg cgcagttttt
     3241 ggaagcgcac ccaaacgtcg gtttggatgt gttcgaccat gtgtccgagg tggatgctgc
     3301 cgttggcata gggcagggcg gaggtaacga ggattttgcg tgtcatattg tgctttgcaa
     3361 acaatgggta aaggcggatt ataccgcaaa tcaaacgggg aaatgccgtc tgaagcctga
     3421 aaaatcaggc ttcagacggc atgttcgcaa acaatccgga cttattcgac cgtaacggat
     3481 ttcgccaggt tgcgcggttt gtctacatcg gtgccgcgtg cgagggcgac atggtaggaa
     3541 aggagctgca cggggatagt atgcacgacg ggggacagtt tgccgacgtg gcgcggtgcg
     3601 cggataacgt gcacaccttc ggtggcatta aaattgctgt cgaggtcggc aaagacgaaa
     3661 agttcgccgc cgcgcgcgcc gacttcctgc atattggctt tgactttgtc caacaggctg
     3721 tcgttgggtg cgatgacgac gacgggcatg ttttcgtcca ccaaggcaag cggcccgtgc
     3781 ttcagttcgc cggcaggata ggcttcggcg tggatgtagg tgatttcctt cagttttaac
     3841 gcgccttcga gggcaatcgg gtaatggatg ccgcgcccca aaaacagcgc gctggttttc
     3901 ttggcaaact gttgcgccca agcggcaatt tgcggttcga ggtttaaagc gtgctgcacg
     3961 ctgccgggaa gctggcggag ttcttcggtg taacgtgctt cgtcttcttc ggaaaccaaa
     4021 ccgcgcactt tcgccagcgt taccgccaaa ccgaacagcg caaccagctg cgtggtaaat
     4081 gctttggttg aggcgacgcc gatttccgca ccggcacggg tataaagcac gaggctgctt
     4141 tcgcgcggca gggcggattc catcacgttg cagatggaga ggctgtggcg gtgtcccaag
     4201 gatttggcgt atttcaacgc ctccatcgtg tccagcgttt cgccggattg ggaaatggta
     4261 atgaccagtt ggtccggatc ggcaatcacg ctgcggtatc ggtattcgct ggcgatttcg
     4321 acatcggccg ggattttggc gatggattcc aaccaatatt tggcggtcag cgcggcgtaa
     4381 taggacgtgc cgcaggcaag gattttgacg ctgcggatgc tttcaaacac gcttttggcg
     4441 tttttgccga agttttcggg gatgaagccg ccgtcgagga aaacctccgc cgtgtctgca
     4501 atcgcgcgcg gctgctcatg gatttccttt tgcataaagt ggctgtacgg tcccagttcc
     4561 aaagaggcga gcgagagttc ggataccttg actttgcgtt cggcaggcag gccgttttta
     4621 tcggtcagcc ttttgatgcc gtctgaagcc agcagcgcga tgtcgccgtc ttcgaggtac
     4681 gccacgcggc gcgtaaaggc gatgacggcg gatacgtccg aagcgataaa ggtttcatcg
     4741 tcgcccaaag cgaccaaaag cgggcagccc atacgcgcca caactaattc atcaggcttg
     4801 tcttgggcaa taaccgcgat ggcgtatgcg ccgtggaaac gtttgaccgc ttcctgcaca
     4861 gcttcaacca atttgccgcc gttttgcgcg tattcgtgat tgatgctgtg tgcgatgact
     4921 tcggtatccg tttgcgattc aaaacggtat cccaaacctt ccaagcgttt gcgttcgctt
     4981 tcaaagtttt cgatgatgcc gttgtggaca accgcaatca tcccgccgct gatgtgcggg
     5041 tgggcgttcg gctcggtaac gccgccgtgt gtcgcccaac gcgtatgtcc gatgccgatg
     5101 ccgccgctga tgcctttttc gcgtgctgcg tcttccataa gctgcacgcg tccgacgcgg
     5161 cgcacgcgtt tgattttgcc gtcggtgttg acggcaatgc ctgatgagtc ataaccccgg
     5221 tattcgaggc gtttgaggcc gtcggtcaga aaatcgacga cgttgtgatg ggcgcggatg
     5281 gcgccgacga taccgcacat aactgttcct tagtatccgg ttgaaaaaaa acaggcgcgg
     5341 acggctcccg tgccgcacct tccgcttcgg attataaacc gcctcccgcg ccgaaaaaca
     5401 gcaaaatgcc gtctgaaggc ttgggcttgc tcaaaaaagg gagggatttc cccgcctgac
     5461 cgggatgggc gttcagacgg cattgcctgc tgccggttta tagtttttac aaatcaatat
     5521 tgacaagctg aaaaaaaaaa acaatatact cgctcggtct taatgttaac ggagtatgga
     5581 aatgaaacaa atgcttttgg ccgtcggcgt ggtggaggtg ttggcgggct gcggcaagga
     5641 tgccggcggt tacgagggtt attggcgcga aaaatcggac aaaaaagagg gtatgattgc
     5701 cgtcaaaaaa gaaaaaggca attacttcct taataaaatc aacgtgttta caggtaagga
     5761 agaatctatg cttttgtctg aaaaagacgg cgcgctttcg ataaacacgg ggatagggga
     5821 aatcccgatc aaactttccg atgacgggaa agagctgtat gtcgaacgca ggcagtatgt
     5881 caaaaccgat gcggcgatga aggacaaaat catcgcccat cagaaaaagt gcggacaaac
     5941 ggcacaggca tatctcgacg cgcgaaatgc gttgccgtca aaccagacgt atcagcagca
     6001 tcaggcggcg atcgagcagt tgaaacggcg gtttgaagcc gagtttgacg aattggaaaa
     6061 agaaatcaaa tgcaacggca aaccgacatt gttgttttag cgtaggacaa ccgggaggat
     6121 gccgccgtcc gaatcggacg tgcggtttct gtaccggtac gggcgggcag gaatgtccgc
     6181 tttttttgtt cggacgcgtt tgaatacccg tttgattccg tccgtttgca aagggtattt
     6241 ttgttcgggc ggaaa
//
LOCUS       DAXHPG010000124         6023 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXHPG010000124 DAXHPG010000000
VERSION     DAXHPG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.124"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="ACFM6F_001653"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="ACFM6F_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC6165036.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="ACFM6F_001654"
     CDS             complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="ACFM6F_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC6165037.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAYEHEMAIRHASEAGITGSNDSQH"
     gene            complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="ACFM6F_001655"
     CDS             complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="ACFM6F_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC6165038.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1350 a         1705 c         1621 g         1347 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcaccct ccgccgcata gctgctgcgt
      181 tccgcatcga gcaagtcgag cgcgccggat acgccgtgct tgtaacgcag gccgaccaag
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccagc
      301 tgctcgcgcg ccgccaatgc gtttgccacg tcttgaaatg cggattggac ggcggattca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat caaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatagac ggcgcgaacg accaaacgcc cgtgccgctt
      481 ttgaacaacc cacccaattc ggcagaaccc gtaccgacgg ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcggcgcgta tgtcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagtttt
      661 tcaacaaaaa actgcttgtc caacggcaaa ccggcgggca ggtcgtcggg tatcggttgg
      721 ttaatcaagg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat tagggcttcc tgctgacgta gggcgacggc ggaaatcacg
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcgtaa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctgct gcttacattg ccgccgctca agctgccttg gcgcgagtcg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatac ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcatgcc aacctaaatc gaccgcacgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggcggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaaagtcc tgatgccgtc tgaaaacccg taggcgttca
     1501 gacggcatgg ttgcttaatg ttggctgtcg ttcgaaccgg ttatgcccgc ttctgatgca
     1561 tgtctgatcg ccatttcatg ctcgtatgca gtttctttga agaatttgcg caccaccaca
     1621 tagaaaagcg gaacaaggaa cacggacaag agcgtgccga tgagcatccc ccagaatacg
     1681 gttgtaccaa tggcgcgctg gctggcagaa cttgcaccgc cggcaatata caggggaacc
     1741 acgcccaaaa taaaggcgaa cgaggtcatg ataatcggac ggaaacgcag gcgggctgct
     1801 tccaaagcgg cttcaaccgc gcttttccct tgcgcttgaa ggtctttggc aaactcgata
     1861 atcaaaatcg cgtttttcgc actcaaaccc atcacggtaa cgaaaccgac ttgaaagtag
     1921 atgtcgttgg cgaacgaggg aacgctgccc aacagtcctt caaacaggtt gcgcccggtt
     1981 acgcccgcag ccgcaccgat caaacccaac ggaatcacaa ggatgaccgc cagcggaatc
     2041 gaccagcttt cataaagcgc ggcaagtacc aaaaatacgg ctgcaaccgc caaaccgtac
     2101 aaaatcaggg tttgcgagcc gccttttgcc tcttcgcgcg actgtccgcc ccattcgaag
     2161 ctgtaaccgc cgcccaattc gtcaaccatt ttttgaaccg ccgccatagc ctgcccggtg
     2221 gaaacgccgg ttgcaggcga agcggacagc ttcatcgaag gataaccgtt gaagcgtacg
     2281 ctctgttccg taccgttttc ccaagaaaca gtagcaatgg tggaaagcgg tacggcgacg
     2341 ccggatttgt tcggcacggt caggttcaaa atatcggcag gctgcatacg ggcatcctcg
     2401 tcggcctgca ccatcacgcg ttgcagacgg ccttggttcg ggaagtcgct gacataagac
     2461 gaactcagcg cacttgccaa tgcggtgcgg atgtcggcaa acgaaatgcc ttgcgccgcc
     2521 gcggcggcac ggttgatgtc gattttcaac tgcggcgagt cttccaaacc gccggcacgg
     2581 acggtgctgg ggtcaaacaa accgctggca cgcattttct gaatcaactc gttgcgcttc
     2641 gccagcaatg cggtatggcc ggtattgttg cggtcttgca ggttgatgct caggcccgaa
     2701 ccattgccca actccaaaat cggaggcggg acgacggcga tgccaaaacc gtctttaagc
     2761 gtccccatca tcatacccgt cagcttgccg gcaatcgcaa cggcatcgct gccgggcgcg
     2821 gtacgctcgt tccaatcttt caatatggca aaacccatcg ccatattctg accgctgccc
     2881 gaaaagctga agccggaaac ggtaatgatg ttttctattt caggaatgct tttcgccagt
     2941 tgggtaactt gcgccaaagt cgcattggtg cgctcttggg tcgctcctgc aggcagttgt
     3001 acgctgacca tgacgaagcc ttggtcttcg gtcggcagga acgaagtcgg caggcgcata
     3061 aacaggaaca cgcccacaac cgccaagccg atatagacaa ccatcatgcg gaaagtctta
     3121 cgcagcactt tggcaacccg gccttcgtaa ccgtgcgtcc aactgttgaa tttcttgtta
     3181 aaccagccga agaaaccttt tttctcttcg tgatgccctt tcgggattgt cttcaacatt
     3241 gtggcacaca aagcaggggt aagggtcagc gcaaggaagg cggaaaatgc gattgatgac
     3301 gccatcgtca gggcaaactg tttgtaaata ttgcccgtcg ccccgctgaa catcgccaac
     3361 ggtacgaaca cggaaatcag aacggcggta ataccgatga ccgcgcccga aatctgaccc
     3421 atcgcttttt tggtcgcttc tttgggcggc aagccttcac ccgccataat gcgctcgacg
     3481 ttttcaacca ccacaatcgc gtcatcgacc acgatgccga tgaccaaaac catcgcaaac
     3541 atggtcagta cgttaatcga catgcccata taagagatga aggcgaaacc gcccaacagc
     3601 gaaatcggta cgacgatggt cggaatcagc gtataacgga tgttttgcag gaagagatac
     3661 attacgacaa acaccagcac catcgcttcg attaaagtat gaatcacttt ttcaatcgaa
     3721 atttcgacga atttggaagt atcgtaaggg gttttccagc tcataccctg aggaaagtat
     3781 ttttccaacg tcgccatgcg ttctttaacc gcctttgccg tcgccatcgc attgccgctg
     3841 ttggacagca tcaccgccat accggtggta tttacaccgt tcagacgggt tgaggaagaa
     3901 tagtcttcca tacccagtcc gacccttgcc acatccttca ggtaaacatt agaaccgtcg
     3961 gtattggcgc ggaggatgac gttgccgaat tcttctgccg tacccaactg cccttgcgcc
     4021 gttacggtag ccgtaaccgt ctgtccgcga acggcgggaa gcgaaccgat agaacccgct
     4081 gaaatctgga cgttctgggc ggacagcgca ctgccgatat cggcaaacga caaattgtag
     4141 ttttgcagtt tcttaggatc aacccaaatc cgcatcgcgc gttgcgcgcc gaacaggcgt
     4201 acctgcccca cgccttcgat acgctgcaac tcgggaacga tattacgctg cgcgtagtcg
     4261 ttcatctctt cggttgactg cacatccgac gaaagcatca caatcatcag gaaattggaa
     4321 cgcgccttgg atacggttac gccgtattgc tggacggttg ccggcagcgt gctcaatact
     4381 tcggaaagct tgttctgcac ttccacctgc gccagattct cgtcggtatc gggcgtaaag
     4441 gtcaggctca cgctgccgct gccgctcgaa tcggcagaag tggacagata atccaaacct
     4501 tccacgccgt tcatattccg ctcgatcacg gaaagcacgc tgtcttccat tacctgcgcg
     4561 gacgcgcccg gataagtggc cctcagggtg atggtcgggg cggcgacaga cggatattgc
     4621 gaaacaggca ggcttttaat gccgaaaata cccgccgcaa taatgaaaat cgaaataacc
     4681 cacgcgaaaa tggggcggtc gataaaaaat ttagccatcg atgccttcct tattccgctt
     4741 cagaagcagt tttggcttca gatgccgtct gaacgccgga ttgaggcgcg gcggcttggt
     4801 tttcagacgg cgcccattct ttgggcgtta cctttttcgc acccgttata ccggcgatac
     4861 tgatgccttc cacaaccacc ttgtccccgt ccttcagacc cgacgtaaca atccaattcg
     4921 taccctgctg ttgcgcgacc gttacctcgc ggggttccat accgccttgg gcgttcacaa
     4981 tcatcacggt atctttcgca ccgcgcgtta ccgcctgctg cggcacgatg aatgcgttat
     5041 ccaccgccac ttggtccatc agcacgcgca catacaggcc gggcatcagg atattctgat
     5101 cgttcggtac ggcggcgcgc agggtaatct gaccggtcga ttcgttgacg gtcggatcgg
     5161 caaacagcag gcggcctttt tcagggtaaa ctgtgccgtc gtcaaatttg atgccgaccg
     5221 caatcacacc atccgccgcc agcagtttgc cttcggctat ctgacggcgc aatttcatca
     5281 cttcggatgc agactgggta acgttcacat acatcggatt ggtttggcgg atggttgcca
     5341 gcacggtcgt atcgcccgca ttcaacagcg taccttcgga aactttggac tgaccgataa
     5401 agccggaaat cggcgcggta atgcgcgaac ggttcagatt aatgccggca gatttgattg
     5461 ccgcctgtgc tgctttgaca cctgcctcgg cagaacgttt cgccgttacc gcagcatcgt
     5521 attcctgccg gctgacggct tcggcggcaa ccaaaggctt gtaccgcgcc aaatccgcat
     5581 ccgctttggc aagcgttgcc tgagccgttg ccagttgcgc gcgtgcgctt tccagacctg
     5641 cttcataagt ggaactgtcg atctgataca gcggctgtcc ggcacggaca taactgcctt
     5701 cttggaacag gcgtttttgg atgatgccgc cgacttgggc gcggacatcg gcggtacgca
     5761 gcgattccaa acgccccggc aactcgacgg tcaatgcgac ggtttgcgga tggacggtta
     5821 cgacaccgac gacgggagcc ggtgcttccc gaccagcagg ctgcccgccc tgcgccgcgt
     5881 ctccgccttt accgcaagac gacagtacca atgcaacggc ggcagccaac gcggccgcac
     5941 gcatcgcctt aaaagcataa aaagccatta tttatcctat ctgtctggtt tgatgtaaag
     6001 ggttttgcca atcaacaggc att
//
LOCUS       DAXHPG010000125         5994 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXHPG010000125 DAXHPG010000000
VERSION     DAXHPG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.125"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            165..1277
                     /gene="nadA"
                     /locus_tag="ACFM6F_001656"
     CDS             165..1277
                     /gene="nadA"
                     /locus_tag="ACFM6F_001656"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC6165039.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            1329..2837
                     /gene="nadB"
                     /locus_tag="ACFM6F_001657"
     CDS             1329..2837
                     /gene="nadB"
                     /locus_tag="ACFM6F_001657"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC6165040.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEASAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NHNDLHLTREGGHTCRRIAHVADYTGEAVMQSLLAQIRRRPHIRVCERQMALDIQTES
                     GVACGLIVLDRRTQETYLIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPYYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYSLGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSESLSSEAGIFSDGIQSAFSRPVLQAFNQRHLGILRNDTGLRC
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(3015..3680)
                     /gene="pgmB"
                     /locus_tag="ACFM6F_001658"
     CDS             complement(3015..3680)
                     /gene="pgmB"
                     /locus_tag="ACFM6F_001658"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFC6165041.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(3693..5951)
                     /locus_tag="ACFM6F_001659"
     CDS             complement(3693..5951)
                     /locus_tag="ACFM6F_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFC6165042.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFDVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFESEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRSGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVSAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPSAWTGYTFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTAALEK"
BASE COUNT         1241 a         1672 c         1747 g         1334 t
ORIGIN      
        1 ccaaagcggt ttgtcggaac gaagcgggtc ggttttacgg tatcgcaaac ctgttttcag
       61 acgaccttgc ttattatact caatttgagc ataatataaa aatatttcaa aatataaacg
      121 gcaaatcttt atgccgtctg aaaaaccagc cataaggaag tctcatgcaa accgccgccc
      181 gccgctcgtt cgactacgat atgccactca tccaaacgcc gacttccgcc tgccaaatcc
      241 gtcaggcgtg ggcgaaggtt gccgacacgc ccgaccgcga gacggcagat cgtctgaaag
      301 acgaaatcaa ggctttgctg aaggagaaaa acgcggtctt ggtggcgcat tattatgttg
      361 atccgctgat tcaggatttg gctttggaaa cgggcggatg cgtgggcgat tctctggaaa
      421 tggcacgctt cggcgcggaa cacgaagccg gtacgctggt ggtggcgggc gtgcgtttca
      481 tgggagagag cgcgaaaatc ctctgccctg aaaaaacggt gctgatgcct gatttggaag
      541 cggaatgttc tttggatttg ggttgcccgg aagaggcgtt ttcggcgttt tgcgaccaac
      601 accccgaccg cacggtggtg gtgtacgcca acacttccgc cgccgtgaaa gcgcgtgccg
      661 attgggtggt aacgtcttcg gtggcgttgg aaatcgtgtc gtatctgaaa tcgcgcggcg
      721 agaagctgat ttgggggccc gaccgccacc tcggcgacta catccgccgc gaaacgggcg
      781 cggatatgct gttgtggcag ggttcgtgca tcgtccacaa cgaatttaaa gggcaggagc
      841 tggcggcgtt gaaggcggaa caccccgatg cggtggtgct ggttcatccc gaatcgccac
      901 aaagcgtcat cgaactgggc gacgtggtcg gctcgaccag caaactgctg aaagctgccg
      961 tatcacgccc cgaaaaaaaa ttcatcgtgg cgaccgattt gggcatcctg cacgaaatgc
     1021 aaaagcaggc gcccgacaaa gaatttatcg ccgcgccgac ggcgggcaac ggcggaagtt
     1081 gcaaaagctg cgcgttctgc ccgtggatgg cgatgaattc gctgggcggc atcaaatacg
     1141 ccctgacaag cggacgtaac gaaatcctgt tggacaggaa gctgggcgaa gccgccaaac
     1201 tgcctttgca gcgtatgctc gacttcgcgg caggactcaa acgcggggat gtgttcaacg
     1261 gcatggggcc cgcctgattt gccgtccata ttgccgtttc agacgacctc tccaacaagg
     1321 acaacaccat gcaaaccgat tgcgacgtat tgattgccgg aaacgggctg gcggcactga
     1381 cgctcgccct gtcgctgcct gaatcgttcc gcatcgtcat tttgtgcaaa aaccggctgg
     1441 acgacaccgc cagccgtcat gcgcaaggcg ggattgcggc ggcgtggtcg ggagaggacg
     1501 acatcgaaaa acacgtcgcc gatactttgg aagcgagcgc aggtttgtgc gacgaagccg
     1561 ccgtccgcac catcctgtcg cagggcaaac cggcaatcga atggctgttg gcgcagggtg
     1621 tggcgttcga ccggaatcat aacgacctgc acctgacgcg cgaaggcggg catacctgcc
     1681 gccgaatcgc ccacgtcgcc gactacacgg gcgaagccgt catgcagagc ctgcttgccc
     1741 aaatacgccg ccgcccgcac atccgcgttt gcgagcggca gatggcgttg gacattcaaa
     1801 ccgaatcagg tgtggcgtgc ggactgatcg tccttgaccg ccgaacgcaa gaaacctacc
     1861 tcatccgcgc ccgccatacc gtactcgcag gcggcggctt gggacagatt tacgccgcca
     1921 ccaccacgcc gcccgaatgc acgggcgacg ccatcgccat ggcgatacgc gcaggctgcg
     1981 cagtcgaaaa cctcgaattt atccaattcc accccacagg cttggcaaga tcgtctgaaa
     2041 acggccgcac cttcctgatt tccgaagccg tgcgcggcga aggcggcatc ctgaccaacc
     2101 aagcgggcga acggtttatg ccgtattacg accgccgcgc cgaactcgcg ccgcgcgaca
     2161 tcgttgcccg cgccatcgcc gccgaaatcg ccaaacaaac gcaagacttc gtctcactcg
     2221 atatcagcca tcaacccgca gcgttcgtcc gccggcattt cccgtccatc catcggcact
     2281 gtttatccca atgcggcctg gatatcacgc gccaagccat ccccgtccgc cccgtgcaac
     2341 actacacctg cggcggcatc caaaccgacc cctgcggcag aacttccctg ccgcagctct
     2401 actccttagg cgaaaccgcc tgcaccggct tgcacggagc caaccgcctt gccagcaact
     2461 ccctgctcga atgtgtcgtt accgccagac ttgccgccca aaccatcgca gacggacaag
     2521 cattccaagc caaaacgtct ccaaggccgt ctgaaagcct ctcctccgaa gcaggcatct
     2581 tttcagacgg catccaaagc gcattcagcc gccccgtcct gcaagcgttc aaccaacgcc
     2641 atctgggcat cctccgcaac gataccggtc tgcgctgcgc catcgcccag ctgcggcttt
     2701 ggaaacaaaa ccaaaccgaa ccgcacaccg cgtccgaata cgaaaaccgc aacctgctcg
     2761 aatgcagcct cgccgtcgcc caagccgcat acaggcggag acagaacatc ggcgcgcatt
     2821 ttaatagtga ttgttaaggt tggctggtta gaaaaaaaat cgttcaaata aatttgaata
     2881 gcatggttaa gaaattggca tttagttttt agggttgcgt aagttgggtt atcaatctgt
     2941 tctaaaagca gaaaggccgt ctgaaacact gttcagacgg ccttttactt ccttgacact
     3001 ttatctgacg cgttttacct gcccgactgt gcccacacgt tttgcaggta ggcgtaggtc
     3061 agctcggcgg tatcggacac cagcgcgatg tcgctgccca agtcttcggc tttgcccacg
     3121 ccgataggca aggcgccggc ggctttgatg gcggcgacac ctgcggcagc gtcttcaatg
     3181 ccgatgcagc ggcggatgtc cgcgcccacg ccctcggcgg cggcgaggaa gatgtcgggg
     3241 gcgggtttgg aatgcgcgac ggcggcaggg tcggcgacgg tgtcgaagaa gtgggtcagc
     3301 cccatgcgtt cgagcaggaa ggggccgttt ttactggcgg acgcaagggc gatttttttg
     3361 ccgtttgctt ttaatgcttc cagcagcggc aaaatgccga gatacacgtc ttcgggtttg
     3421 actgcctgaa tcatctcgac gtagttgtcg tttttgcggc gggtcagttc ggcgaactcg
     3481 gcttcgccga cggttttgcc gccgtgtgcg aggatgcgtt tgagcgaatc gtcgcgcgac
     3541 acgcctttga gctgctcgtt aaacttgcgg tctatgccga tgcccagttc ttcggcgagc
     3601 tttttccatg cgcggtagtg gtattcggcg gtgtcggtga tgacgccgtc gagatcgaag
     3661 agcactgcgg taaacgtcat tttgcgccct ccttattttt ccaacgcggc ggtatggctg
     3721 ccgttgagtg tgatgtcttt gccgtacacc tgcaaatcga gcggctcgcc tttgagcagg
     3781 gtgaagacga cgttttcttt gccgacggcg actttaatca gacggcctcg gtagttgata
     3841 tggaaggtgt agcctgtcca cgcgctcggc aggaacggtg cgaagctgag tttaccgccc
     3901 caagttttca tttgggcgaa accttggacg atggcgagcc acgagccggt catagaagta
     3961 atgtgcaggc cgtcttcggt gtcgttgttg tagttgtcca agtccaggcg tgcggtgcgc
     4021 cggtacattt ccacggcttt ttcttctttg cccagttcgg cggcgagaat agagtggata
     4081 cacggcgaca acgagctttc atgcacggtc atcggttcgt agaagtcgaa gttgcggcgt
     4141 ttttcgtcga tattgaaacg gtcgccgaag aaatagatgc cttgcaatac gtccgcctgt
     4201 ttgataaagg gcgaacgcag gattttgtcc cacgaccatt tctggttgag cggcaaatcg
     4261 tcgggcgaaa gcgcggacac ggggcggatg tctttgtcga ggaagccgtc gtgttgcacg
     4321 aatacgccga gttcttcgtc atgaggacgg tacatattcg cgctgatgtc cgcccatttt
     4381 tccaactcgg cggcgctcac gttcaaatcc ggacgcgggt atttcgccag ggcttcgcgg
     4441 gtgtagtcca atacccatgc ggcgagggtg ttggtgtacc agttgttgtt gatgttgttt
     4501 tcgtattcgt tcgggccggt tacgccgtga atcatgtatt tgccgctgcg tttggagaag
     4561 tggacgcggt ccgcccagaa gcgggacact tcgaccaaga cttccaagcc ttctttggca
     4621 agatagcttt cgtcgccggt gtagttggtg tagttgtaga tggcgtaagg aatcgcgcca
     4681 ttgcggtgga tttcctcgaa ggtgatttcc cattcgttgt ggcactcgat gccggtaaac
     4741 gttaccatcg gatagagtgc gcccgccaag ccctgttcgc gcgcgttgtg ctgcgcctgc
     4801 ggcagttggt tgcggcggta ttgcagcagg ttgcgggtaa cttcgggttc cgccagtgcg
     4861 aggtagagcg gtacggcata ggcttcggta tcccaatagg tcgcgccgcc gtatttttcg
     4921 ccggtaaagc ctttggggcc gatgttcagg cgcgcgtctt cgccgtagta ggtggagaac
     4981 agttggaaca gattgaaacg gatgccttgc tgcgcttcgt cgctgccttc gatgaccacg
     5041 tcggcgattt cccaacggtg cagccagcct gctttgtgcg cgtccagcaa ggtttcaaac
     5101 gcaacgcctg cgactttttc cgacaaggcg cggcctgcgg ctttcactgc ttccaagccc
     5161 tgataatcgc ggctggtggt aacaatcacg cgtttttcaa aggttttggg cgtgccgccg
     5221 acttcggact cgaaagaatt ggagacctgc cagtcggttt ggctgccgcc gagggctttg
     5281 aagctgccgg cgaaagtttg ctcggcgttg acgataaatt gttccacgcc gaagggattg
     5341 gcgacggttt gggcggcaat gtaggagaga ctgtctgaaa cgcctttgtc caatacctgc
     5401 cagaattttt cttcgtagtt ggagtcttcg tttttcacgt cggcatcgat gatggaatcg
     5461 atgcggactt ggtgggtttt gccgtcaacg gatacggctt cccagcggat gaccgccaac
     5521 tctttttgcg cgacggacag gaatttacac acatcgaaac gcacgccgaa tacggtgaac
     5581 gagcggcgca acacgccgtg ctgcatatcg agttcgacgg agaagccggc aacatcgttt
     5641 ttcgccaagt ccacctcttg tccgtcgaca aagattttga ctttgctgaa attgagcgcg
     5701 ttgatggctt taccgaaata tttggggtag ccgtttttcc accagccgac gcgggttttg
     5761 tcgggaaacc acacaccggc gatgtaggta cccaagtggc tgtcggcgga ataggtttcc
     5821 tcaaagctgc cgcgcatacc catatagccg ttgcccaaac tggtcaggct ctcttgcagc
     5881 cgtttgtgtt ctttttccag ctttgccgaa cgcagcgtcc aagggctgat ttccataatt
     5941 cttgtgtaca tttatgaagc tcctgtttgg attgatttga gggaatggtg aaat
//
LOCUS       DAXHPG010000126         5991 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXHPG010000126 DAXHPG010000000
VERSION     DAXHPG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.126"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..79)
                     /locus_tag="ACFM6F_001660"
     CDS             complement(<1..79)
                     /locus_tag="ACFM6F_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6165043.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="ACFM6F_001661"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="ACFM6F_001661"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC6165044.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKTVSI"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="ACFM6F_001662"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="ACFM6F_001662"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC6165045.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="ACFM6F_001663"
     CDS             complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="ACFM6F_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC6165046.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSGHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQGLSQRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVKRGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLVGKSVEALKNYLSLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4804..5868
                     /gene="fba"
                     /locus_tag="ACFM6F_001664"
     CDS             4804..5868
                     /gene="fba"
                     /locus_tag="ACFM6F_001664"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC6165047.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
BASE COUNT         1349 a         1824 c         1563 g         1255 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccaaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatac agccgtcctg
      121 accgattaaa ttcatatttt aaaacaatat tctgtttaca aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgttga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgcccaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctcattc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtt aaagaaatca acctcttagg ccaaaacgtc aatgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgacttta
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acattgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgca cgacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaacgat gatcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccaact gcaagcccgc accgccaaca
     1501 accgcgtcgt caactttacc ggcacgcccg atatgatcaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaaaccgt cagcatttga tttcccccgc
     1621 cgcaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacgaagcgc
     1981 atatcggcga cggtggcgtt cagtttttcg gcaaccgcca atgcgggggc gaccatgctg
     2041 ccaaaggcaa tgaaggcagt tttcccacct tcgcggcgga taatgccctt gccgatttca
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg cgtctgcctg atagcaggtc gaaagcagca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat attcggaatg cagcgcaaga agctcaaatc gtacaaaccg
     2281 gcgtgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggc
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catcccttcg caagccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg cgatgccgac atcgaaatag cggtcgggga atcgttgttc aaactcaacc
     2521 aagccgctgc cctcgcgcat ggcgggggta atcgcaacca gtcgggaatc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcggc gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgctt tctttaggca ggttggcgac ggcgtggtat
     2701 ttgacgggat cgttttcggc gagtttgtag ccgttgcctt ttttggtaat gacgtgcagc
     2761 aactgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt gtagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcaattca aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caagagtttg tctgccgccg ccatacccaa agccgcgccg
     3241 atggaagtgg acgaatgccc cacgccgaac gcgtcgtatt ccgactcaca acgtttcgga
     3301 aaacccgcca agccgccgta ttggcgcatc gtgtgcattt ggtttttcct gcctgtcagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccacacca gcttgtcttc gggcgtgtcg
     3421 tacacatagt gcagggcgat ggtcagttcg accgcgccca gattgctggc gaaatgaccg
     3481 ccggtctgcc cgacagattc cagcagaaag gcgcgcagct cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggtcaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gtttggtgta aatctgcgtg
     3781 gtcgaaaggc tgctgtgtcc gagcagctcc tgcaccgccc ggatgtcgcg cgaagcctgc
     3841 aacaggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcga cagatagttt
     4021 ttcagggctt ccacgctttt gccgaccagc ggcacctgcc gctgcttgcg ccctttgccg
     4081 gtaacgtgta cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagc tcgaacaggg cgtgatcgcg caccgccagc
     4201 gggtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagggct
     4261 ttgggtacgc gctcgggctg cttcggcggt ttgatgtcgg cggtcgggtc ggtatgcagc
     4321 agaccgcgtt tgaccagcca ggcgcaatac tgccgccaag acgacagctt gcgcgccagc
     4381 gtccgcgcat tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgttgc
     4441 gacaagccct gcggcacgcc gcctgcatct tcagacggca tttgtgccaa cagcgcaaac
     4501 agttcttcca aatcgcgccg gtatgccgcc accgtgtgcc ccgacttgcc ctcgcgcacg
     4561 atgttttcca aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgcccca
     4621 cacaaatatc aaaaaaacag tgaaaacatt atcataaacg acacaccaaa accccgaacc
     4681 gcccaaaatc cgacccgtcc gcccgccgtt atcgttaaca taaaatcaat gagccgttta
     4741 tggttttttg ccgcaaaaaa cattataatc cgccttattt acctattgcc caaggagaca
     4801 caaatggcac tcgtatccat gcgccaactg cttgatcatg ctgccgaaaa cagctacggc
     4861 ctgcccgcgt tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac
     4921 caagtcaacg cgcccgtcat cgtacaggcg agtgcaggtg cgcgcaaata tgcgggcgcg
     4981 ccgtttttgc gccacctgat tctggcggct gtcgaagaat ttccgcacat ccccgtcgtg
     5041 atgcaccaag accacggcgc atcgcccgac gtgtgccaac gctccatcca actgggcttt
     5101 tcctccgtga tgatggacgg ctcgctgatg gaagacggca aaaccccttc ttcttatgaa
     5161 tacaacgtca acgccacccg taccgtggtt aatttctccc acgcttgcgg cgtatccgtc
     5221 gaaggcgaaa tcggcgtgtt gggcaacctc gaaactggcg aagccggcga agaagacggt
     5281 gtaggcgcag tgggcaaact ttcccacgac caaatgctca ccagcgtcga agatgccgtg
     5341 cgtttcgtta aagataccgg cgttgacgca ttggcgattg ccgtcggcac cagccacggc
     5401 gcgtacaaat tcacccgtcc gcccacaggc gacgtgttgc gtatcgaccg catcaaagaa
     5461 atccaccaag ccctgcccaa tacacacatc gtgatgcacg gttccagctc cgttccgcaa
     5521 gaatggctga aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg
     5581 gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca aagtcaacat cgataccgac
     5641 ttgcgccttg cttccaccgg cgcggtacgc cgctaccttg ccgaaaaccc gtccgacttc
     5701 gatccgcgca aatatttgag caaaaccatt gaagcgatga agcaaatctg cctcgaccgc
     5761 tacctcgcgt tcggttgcga aggtcaggca ggcaaaatca aaccggtttc cttggaaaaa
     5821 atggcaaacc gttatgccaa gggcgaattg aaccaaatcg tcaaataaca ggttgcttgt
     5881 aaacaaaatg ccgtctgaac cgttgttcgg acggcatttg atttttgctt ctttgacctg
     5941 cctcattgat gcagtatgca aaaaaagata ccataaccaa aatgtttata t
//
LOCUS       DAXHPG010000127         5962 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXHPG010000127 DAXHPG010000000
VERSION     DAXHPG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.127"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(41..964)
                     /gene="truB"
                     /locus_tag="ACFM6F_001665"
     CDS             complement(41..964)
                     /gene="truB"
                     /locus_tag="ACFM6F_001665"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC6165048.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(997..1368)
                     /gene="rbfA"
                     /locus_tag="ACFM6F_001666"
     CDS             complement(997..1368)
                     /gene="rbfA"
                     /locus_tag="ACFM6F_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC6165049.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKTPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            1559..2803
                     /gene="clpX"
                     /locus_tag="ACFM6F_001667"
     CDS             1559..2803
                     /gene="clpX"
                     /locus_tag="ACFM6F_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC6165050.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(2906..4102)
                     /locus_tag="ACFM6F_001668"
     CDS             complement(2906..4102)
                     /locus_tag="ACFM6F_001668"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC6165051.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALADALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLCRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            4506..5006
                     /locus_tag="ACFM6F_001669"
     CDS             4506..5006
                     /locus_tag="ACFM6F_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165052.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            5091..5645
                     /locus_tag="ACFM6F_001670"
     CDS             5091..5645
                     /locus_tag="ACFM6F_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165053.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
BASE COUNT         1418 a         1588 c         1609 g         1347 t
ORIGIN      
        1 aacacattat tcaaacacag cctgtatttt taaccgccgt tcaggcggcg gatgccgccg
       61 tgttcatcag gcgcaaggct ttcagacggc atatttcttt ttgatattcc gccagaccga
      121 caaagcggcc gttttccgtg taaacgcgta ccggcgtgtc ggaaggcagg tcttcttcga
      181 aacgcggacg ttgtccgcag tggagcatat ggacggcata atcgtttaaa acggtttggg
      241 gaaagtgtga aaccaatacg tcgcagggta gcagcaagct gtcgcgttct gtttcgttca
      301 aatttgccaa ggcctcaagc gtgtggcttt gggcgatggt aaagccggcg gtttcagtgc
      361 ggcgcagggc ggtcaggtgg gcgaacgtgc cgatgtgttt ggcgatgtct tcgctgaggg
      421 tgcggatgta ggtgcctttg ctgcaacgta cgtcgatgac ggctttgggc gcgtcaaatt
      481 cggcaatatc gatggcgtaa acggtaatgt cgcgcgcttt gcgttcgatg acgatgcctt
      541 tgcgggcgta ttcgtacagc ggtttgcctt cgtgcttgag cgcggaaaac attggcggca
      601 cttggcggat gttgcctgtc agtgcttggc aggccgtctg aaattcggct aaggaaatat
      661 cggcgcgggc ggtggcaatg atttcgcctt cggcatcacc cgtgctgctg gcttcgccga
      721 gtttcagcgt ggcggtgtag gctttgtcgg catccagcag gtattgggcg aacttggtcg
      781 cttcaccgaa gcagacgggc aaaagtccgg ttgccaaagg gtcgagcacg ccggtatgtc
      841 cggctttttc ggcatgaaac aaacgccgcg ctttttgcag cgcggtgttg ctggaaaggc
      901 cttcgggttt gtccaagagc aaaacaccgt tgacggcacg tttttggggt ttgcctgtat
      961 tcatggttcg atgttcggac ggcatgggca tatccgtcag tcttcaaccg gtttttccgc
     1021 cgccacttga tcgataaggg cggacaggtt caaaccgcgt tccaaagatt cgtcgtattt
     1081 gaaatgcagt tcgggcgttt tgaacagctt gatgcgtttg gccaattcgc tgcggaggtg
     1141 tccgcgcgcg tgttccagca cttcttccgt aatttcgcgc gcgtcttggt ttaaaatggt
     1201 gtagaacacg gtggcgtggc tgtaatcgcg ggtaacttcg acttcgttga cggtaatgaa
     1261 gccggcgcgc gggtctttca gtccggtacg gacgagttcg gcaagctcgc gcataatttg
     1321 ttctttgaca cggtcttggc gggcgtagcc gcgttggggt tttctcatga atatcctcag
     1381 atttcagatt tggtttcaga cggcattttt gccgccgggg cggttgcgaa agggcgtatt
     1441 atagcctaat cggcgggatt ttaaggtaat acattgataa gaacggatat agttattata
     1501 cttggcaacc cgaacggcac gtgccgtttt ctgtaacgca tacattgtaa agaaagccat
     1561 gtccaacgaa aaccgtactt gttccttttg cggaaaatcc aaatcacacg tcaaacattt
     1621 aattgagggc gaaaacgcct ttatctgtga cgaatgcgtc tcaaactgta ttgaaatatt
     1681 gcatgaagac ggaaatgacg gcacgccttc ggaaagtgcc ggaggggagc cggaagaatc
     1741 cggcaagctg cccacgcccg ccgaaatcgt cgccaacctc aacgaccacg ttatcggtca
     1801 ggaacaagcc aagaaagcct tggcggtgtc ggtgtacaac cattacaaac gcctgcgcca
     1861 tcctaaagca ggagctaatg tcgaattgtc gaaatccaac atcctgctta tcggcccgac
     1921 cggatcgggt aaaaccctgc tggcgcaatc tttggcgcgc aaactggatg tgccgttcgt
     1981 gatggcggat gccacaactt tgactgaagc aggttatgtc ggcgaagacg tcgagcaaat
     2041 cattaccaag cttttgggca aatgtgattt cgatgtcgaa aaagcccagc gcggcattgt
     2101 ctatatcgac gaaatcgata aaatttcacg caaaagcgac aacccgtcga ttacccgcga
     2161 cgtatccggc gaaggtgtgc aacaagcctt gttgaaactg attgaaggta cagtggccag
     2221 cgttccgccc caaggcggcc gcaagcatcc gaatcaggaa tttatcaacg tcgataccac
     2281 caacatcctg tttatctgcg gcggcgcgtt tgcaggcttg gaaaaagtca tccgccagcg
     2341 tactgaaaaa ggcggtatcg gtttcggtgc gtccgttcac agcaaggacg aaaatgccga
     2401 cattaccaag ctgttcggca tcgtcgaacc ggaagacttg attaaattcg gtctgattcc
     2461 agaattaatc ggacgtttgc ctgtgattgc aactttagaa gaattggacg aagacgcgct
     2521 gattaatatt ttgaccgagc cgaaaaatgc tttggtcaag cagtatcagg cgttgttcgg
     2581 tatggaaaat gtcgaactgg agtttgaaga aggcgcattg cgttccatcg cgcgtcaggc
     2641 aatggaacgc aaaaccggcg cgcgcggcct gaggtccatc gtcgaacgct gcctgctcga
     2701 tacgatgtac cgcctgcctg atttgaaagg cttgaaaaaa gtggtggtcg gtaaggcagt
     2761 catcgaagag ggcagggaac cggaattggt gttcgagtcc tgatggcaaa caaaaccgct
     2821 tcggaacgtt gaaccacgtt tctgaagcgg ttttttgttt ctgatgccgt ctgaagcctt
     2881 cccgccttca gacggcattt gagttttacg gattgtctga tgtcgcggca aattccgtca
     2941 gcgcgtgttc caaacgtcgc aaaccttccg ccatatcctc atcgttcaac agtagcgaag
     3001 gcgcgaaacg caatacgtcc gcacccgcaa ccaaaatcat cacgccgtgt ttcaaggcgg
     3061 cggcggtgat ttcggatgcg cgtccgcgat aggcttcgtc caacacgcag ccgagtagca
     3121 gccccatccc gcgaacttgt gagaacaagc ccgttttcct gcacaaatcc agcaatgccg
     3181 tctgaagttt ctgcccctgt tcacggacat ggtttaaagt ttcgggcgta ttgatgatgt
     3241 cgaatgcgcg gctgccgacc gcacacgcca tcgggttgcc gccgaaagtc gagccgtgcg
     3301 tgcccggttg gaaggcggcg gcaatctttt ctgtcgccag catcgcgccg atcggaaagc
     3361 cgcagcccaa ggcttttgcc gaactcaaaa tatcgggaac aacgccgtaa tgttcatagg
     3421 caaacagcct gcccgtatgc cccatcccgg tttgaacttc gtccaaaatc aacaacgcat
     3481 tgtgccggtc gcacagacgg cgcgcggttt gcaaatattc cgcagtggcg ggcaggatgc
     3541 cgctttcgcc ctgtatcggc tcgatgatga ccgcgcaggt ctgttcgccg acggcagctt
     3601 ccagcgcggc aatatcgttg aacggaacgt gcgtaatgcc ttgcggcagc ggtgcataat
     3661 ccttgctgta tttcggctga ccgccgacgg acacggtaaa cagcgtgcgt ccgtggaaac
     3721 tgttgataca ggcgacgatt tcgctttttc ctccgccgaa acggtcgcgc gcatatttcc
     3781 ttgccagctt caacgccgcc tcattcgctt ccgcgcccga gttgcagaaa aaaaccttgt
     3841 cggcaaaact gtttgcaacc aatttttgcg ccaattcctg cgccggacgc gtcgtataga
     3901 tattggaaat gtgccacagc ttgtgcatct gcgcgtttaa agcatcggca agggcagggt
     3961 ggcagtgtcc cagcgcattg acggcgatac cgcctgaaaa atcaatatat tcacgccctt
     4021 ccgtatccca aacgcggctg cctgaagcgc gttcgggaat catcggggca aaggcgaaat
     4081 tgggggtcag atagttttgc ataattgctc ctgattgcgg ttttgctcgg gattatgcac
     4141 cgtttttcaa agcagataaa gggaaattgc cgacaatatc gggcagcggt aaaaataaac
     4201 cgaaatattt tcaatcggat agatgaaaaa tcaaaaaaat ttcgataaaa tgcttgaggc
     4261 gcggcagcgt tttgatttag aatctcccca atatttttat cttcagacgg catttcatca
     4321 tgcaaggcaa aaaatttaca accgcttatt gctattggta ccacacagac gtgcggtact
     4381 tttttgcgct tgcttgaaat ccatcccata cgcaaaacag gccgctcgaa aatggggcgg
     4441 cttttttgtt accttcacac aggtcgcccg attcggcaac acacgacaga atcaggaaaa
     4501 tgaccatgaa tgcaatgccc caaaccgccg cacaaaacga aaccctgcac acttgtagca
     4561 ttattgtcga tgatgcgcag gatttttccc ccatgcttct cgatgcaggg cgggacgaca
     4621 cacttatcag cgaatcgatg attccgcaaa tccgcaccgt tgccgccctg atagccgccg
     4681 aacggcacga cttcagccgc agcagccccg ccgagtttac cgatgccgcc gatttttttg
     4741 ccgcacggat tctcgtattg ggcgtgcgac gcttccatct ggacgtatcg ctgcttcaga
     4801 ttttgaaaac cgccaacagc cgcgcccgcc gctttgccga aaaacaccgc ctgtttttca
     4861 cacccgcaca agccgaattg agcctgaaca aacacaaaaa ccgccgcctg ctgaccgtag
     4921 aaaccgaaca cgaagtcgaa aacaaaggca acccagtcgc caacagcctc gcatttgtcc
     4981 gcaaactgca caccctgccc ctgtaaacat cacgatttgt cggcagaatg cttgccaaaa
     5041 ttcccgacgg cgatacaata aaaccgattt tccacccaaa caggacacac atgaaaaaaa
     5101 tcatcgcctc cgcgcttatc gcaacattcg cactcgccgc ctgccaagac gacacgcagg
     5161 cgcggctcga acagcagcag aaacagattg aagccctgca acagcagctc gcacagcagg
     5221 cagacgatac ggtttaccaa ctgactcccg aagcagtcaa agacaccatt cctgccgaag
     5281 cacaggcaaa cggcaacaac gggcaacccg ttaccggtaa agacgggcag cagtatattt
     5341 acgaccaatc gacaggaagc tggctgctgc aaagcctggt cggcgcggcg gcaggcgcgt
     5401 ttatcggcaa cgcgctggca aacaaattca cacgggcagg caaccaagac agtcccgtcg
     5461 cccggcgcgc gcgtgcagcc taccatcagt ccgcacgccc caatgcgcgc accagcaggg
     5521 atttgaacac gcgcagcctc cgcgcaaaac aacaggcggc gcaggcgcag cgttaccgcc
     5581 cgacaacgcg cccgcccgcc aattaccgcc gaccggctat gcgcggtttc ggcaggaggc
     5641 ggtaaacccg gcgcgccaat gccgtctgaa gggctttcag acggcatttt gtttccaaga
     5701 gtttgatgtc gggatggcaa ttctgattta cgtgcgttgg cagggcggca ttgtcatgcc
     5761 gccgttgggt tttcagacgg catcatatcg aaccgtcagg tcgtggggtg agcattcttg
     5821 cccaccgttg taagggcggg attttttggt gaacaggaat gcccaccctg catctgaaac
     5881 ccttctgcgt cattcccacg caggaaggaa tttagatatt tgaatttaca gaaattttca
     5941 atcgttgcca aagcggaaaa gg
//
LOCUS       DAXHPG010000128         5931 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXHPG010000128 DAXHPG010000000
VERSION     DAXHPG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.128"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            20..403
                     /locus_tag="ACFM6F_001671"
     CDS             20..403
                     /locus_tag="ACFM6F_001671"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC6165054.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            517..990
                     /locus_tag="ACFM6F_001672"
     CDS             517..990
                     /locus_tag="ACFM6F_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC6165055.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            1116..2219
                     /gene="mnmA"
                     /locus_tag="ACFM6F_001673"
     CDS             1116..2219
                     /gene="mnmA"
                     /locus_tag="ACFM6F_001673"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC6165056.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYEGDVCLGGGIIQSTDKPVIITR"
     gene            2290..3960
                     /locus_tag="ACFM6F_001674"
     CDS             2290..3960
                     /locus_tag="ACFM6F_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC6165057.1"
                     /translation="MKKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPVALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETISFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4072..5706
                     /locus_tag="ACFM6F_001675"
     CDS             4072..5706
                     /locus_tag="ACFM6F_001675"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC6165058.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            5749..>5931
                     /locus_tag="ACFM6F_001676"
     CDS             5749..>5931
                     /locus_tag="ACFM6F_001676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165059.1"
                     /translation="MKTFRRHFCKSGIIKAINLIFMVLSNIDQLNIVKRGVKLKLRPL
                     QNSPKSPKFPPRHLGDF"
BASE COUNT         1556 a         1616 c         1492 g         1267 t
ORIGIN      
        1 acattttgaa aggataccga tggaaccttc ctcctacgcg gcagaaaaaa aaggcaaagg
       61 cggcatcagg cgcgtcatta acgcattcgg ctattcgata gacggcatcg ccgccgccta
      121 ccgttacgaa gcggcattcc gtcaggtttt gtggctgaac gtactgctgg tgtgcgcggc
      181 atttttttgg gtttccgaaa ccgccgtccg cctgccgttg attatcgcgt cttttgtgtc
      241 ggtcattgtc gaactgttca acaccgccgt cgaagctgcc gtcgatcata cttcgaccga
      301 aaaacacgag ctggccaaac gcgccaaaga cgcaggttct gccgcacaat tgttcgcgat
      361 gctgatgttg gcggcggttt ggctgtctgc cctgttcggg taaaacgctt gcagcaggat
      421 ttgtaatcct ttaggattag tatgtggtat ttattattca cttaatccac tataagatta
      481 agcacattcc ccgtctgata ttcaaggagc agaaagatga aaaaattatt ggcagccgtg
      541 atgatggcag gtttggcagg cgcggtttcc gcggccggag tccacgtcga ggacggctgg
      601 gcgcgcacca ccgtcgaagg tatgaaaata ggcggcgcgt tcatgaaaat ccacaacgac
      661 gaagccaaac aagacttttt gctcggcgga agcagccccg ttgccgaccg cgtcgaagtg
      721 catacccaca tcaacgacaa cggcgtgatg cggatgcgcg aagtcaaagg cggcgtgcct
      781 ttggaggcga aatccgttac cgaactcaag cccggcagct atcacgtgat gtttatgggt
      841 ttgaaaaaac aattaaaaga gggcgacaag attcccgtta ccctgaaatt taaaaacgcc
      901 aaagcgcaaa ccgtccaact ggaagtcaaa accgcgccga tgccggcaat gaaccacggt
      961 catcaccacg gcgaagcgca tcagcactaa tctgctgaaa atatttgaaa tgccgtctga
     1021 aaaagcccgg gctttcagac ggcattttta tgactgcctt taaaatgtgt taaaatccgc
     1081 ctttaaaaac cgccgtttcc aaccatattc tacgcatgaa tacgacagca aacccttcca
     1141 atatcatcgt cgggctctcc ggcggtgtcg attcttccgt aaccgccgcc ctgctcaagc
     1201 agcagggtta tcaagtgcgc ggtgtgttca tgcagaactg ggaagacgac gacaacgacg
     1261 aatattgcag catcaaacag gattcgtttg atgccatcgc cgttgccgat attgtcggca
     1321 tcgacatcga catcgttaat ttcgccgcgc aatataaaga caaagtcttt gcttattttc
     1381 ttcaggaata cagtgcgggg cgcacgccga atcccgatgt gttgtgcaac gccgaaatca
     1441 aattcaaatg ctttttggat tacgccgtag ggcagggcgc ggataccatc gccaccggac
     1501 actatgcgcg caaagaagtc cgcaacggcg tgcattacct gctcaaaggt ttggatcgaa
     1561 acaaagacca aagctatttc ctctaccgcc tcaagccttt ccaactcgag cgcgcgattt
     1621 ttccgttggg cggtttggaa aaacccgaag tccgccgcct tgccgccgag ttcaaactgc
     1681 cgactgccgc caaaaaagac agcacgggca tctgcttcat cggcgaacgc ccgtttcgcg
     1741 agtttctgca aaaatacctg ccgaccgaca acggtaaaat ggttacgccc gaagggaaaa
     1801 ccgtcggcga acacgtcggg ctgatgttct acacgctcgg acagcgcaaa ggtttgggca
     1861 ttggcggcgc gggcgaaccg tggtttgttg cggctaaaga tttgacgaaa aacgaactga
     1921 ttgtcgtgca aggacacgac catccgctgc tctatacccg cagccttgtg atgaacgatt
     1981 tgagtttcac gcttcccgaa cgcccgaagg caggacgcta tacctgcaaa acgcgttacc
     2041 gtatggcgga cgcgccttgt gagttgcgct atttggatga agaaaccgcc gagctggtgt
     2101 ttgacgaacc gcaatgggcg gttacgccgg gccagtctgc cgtgctgtat gagggcgatg
     2161 tctgcttggg cggcggcatt atccaatcaa ccgacaaacc cgtcatcatc acgcgataaa
     2221 ggtaatgccg tctgaaacgg ttttcagacg gcattgttcc gctcaattcc actttaaaga
     2281 ccgataccta tgaaaaaaat ctggttagac agctacgaga agggcgtcag tgccgaaatc
     2341 gacatcacgc aatacaattc cgtcagcgac gtattccgcc aaagcgtgga aaaatttgcc
     2401 gaccaacctg cttttcaaaa catgggtaag acgctgactt atgccgaaac cggcaaactg
     2461 gcaacagatt ttgcttctta tctgcaaaac gtccttaaat tgcctcgagg agagcgcgtg
     2521 gcaataatgt tgcccaatgt actgcaatat ccggttgccc ttttcggcat tttgcaggca
     2581 ggtttggtag cggtgaacac caatccgctc tataccccgc gcgagctgga gcatcagttg
     2641 aaagacagcg gcgcgaccgc catcatcgtt ttggaaaatt tcgccaacac gctggagctg
     2701 gtgctgccgc gcacgcagat caaacacgtc atcgtcgcct ctgtcggcga aatgttcggg
     2761 ctgcttaaag gttcgctgat caatttcatc atccggaaaa tcaagaaaat ggtgcccgaa
     2821 taccgtattc gggaaaccat ttcctttcag acggcattga aagaaggggc gaagcacgtt
     2881 ttccaacctg tcgcattaaa ccgcgaagat accgcgctgt tgcaatacac gggcggcacg
     2941 acaggtgttg ccaaaggggc ggtgctgagt cacggcaaca tctgcgccaa tatgcttcag
     3001 gcaaaagaat ggattaaaaa ccagttgcgc gagggcaaag aaaccgttat cgccgccctg
     3061 ccgctgtatc atatttttgc cttaaccgtg aatctgatga tttttgccaa tgccggctcg
     3121 aaaatcatcc tgattaccaa cccgcgcgat atgaaaggct ttatcggaga actgaaaaaa
     3181 cagcgggtta atgtatttat cggcgtgaac acgctgttta acgcgatggt taaccgtcct
     3241 gattttgccg aagtcgattt ttcaggattg cggctgactt tgggcggcgg tatggcgacc
     3301 caaaaagccg ttgccgaaaa atggaaaaaa atcaccggca cgcccatcgt cgaagcctac
     3361 ggtctgaccg aagccagccc cggcgtgtgc tgcaacccct taaacatcga atcatacagc
     3421 ggcagcatcg gtttgcccgt ctcgtccacc gaagtcgaac tgcgcgacgc aaacggcaaa
     3481 gaagtccccg tcgggcagcc gggcgaattg tgggtaaaag gccctcaagt gatgcaaggc
     3541 tactggaacc gccccgaaga aaccgccaaa gccatagacg cgcgcggctt cttggaaacc
     3601 ggcgatattg ccgtcatgga cgaaaaaggc tggttgaagc tggtcgatcg taagaaagac
     3661 ctcgtcgttg tttccggatt caatgtttat ccgaacgaaa tcgaggaagt catcgcgcat
     3721 cacggcaaag tcatggaagt cgcctgtatc ggcgttcccg acgaaaaaac cggcgaggca
     3781 ctcaaagtat tcgtcgtcaa aaaagacccg tcattgacca aagaagaact tatcgccttt
     3841 tgccgcaccg aattaaccgc atataaagta ccgaaaaata tcgaattccg cgacgagttg
     3901 cccaagtcca acgtcggcaa aatcctgcgc cgcgagttgc ggcaaagtac cgggaaataa
     3961 agaaaagatg ccgtctgaaa acagccgtcc accgttcaga cggcatccgt ccgtttgcca
     4021 gaaccgcgcg cttcacgtta aaatcacgca ttccaacatg ggtattccat catgaccaaa
     4081 ttcattttcg taaccggcgg cgttgtctcc tcactgggta aaggtatcgc cgccgcttct
     4141 attgccgcca tcctcgaatc gcgcggcttg aacgttacca tgctcaagct cgatccttat
     4201 atcaacgtcg atcccggcac gatgagcccg tttcaacacg gcgaagtgtt cgtaaccgac
     4261 gacggcgcgg aaaccgacct cgacttggga cactacgaac gtttcatcga ttccacgatg
     4321 acccgccgca acagcttcag cacggggcag gtgtacgaaa acgtcatcgc caaagaacga
     4381 cggggcgact acctcggcgg tacggttcaa gtcatcccgc acattaccga cgaaatcaaa
     4441 cgccgcatcc acgaaggcgc ggcaggttac gatgtggcga ttgtcgaaat cggcggtaca
     4501 gtcggcgata tcgaatcttt gccattcttg gaagccatcc gccaaatgcg cagccagctc
     4561 ggccgcaaca atacattgtt tgcccacctg agctatgttc cttacatcgc cgctgcaggc
     4621 gaaatcaaaa ccaagccgac ccagcacacc gttaaagaaa tgttgagcat cggtttgcaa
     4681 cccgacatcc tgatttgccg tatggacagg acaatgcctg cggacgaacg ccgcaaaatc
     4741 gccttgttct gcaacgtgga agaacgcgcg attgtcggca gctacgatgt ggacagcatc
     4801 tacgaatgcc ccgaaatgct gcacgaccaa ggcatcgata acatcattac cgagcagttg
     4861 cagcttaatg tgcagcaggc ggatttgacc gcgtggaaaa aaatcgtcca cgccatccaa
     4921 aacccgaaac acaccgtcaa aatcgccatg gtcggcaaat acgtcgattt gaccgagtcc
     4981 tacaaatcgc tgatcgaagc cttgaaacac gcaggcattc acaccgaaac cgacgtacaa
     5041 attacctttg ttgacagcga aagcatcgag aaaaacaacg gcgacgtttc tatgctcaaa
     5101 gatatggacg ccatcctcgt tcccggcggt ttcggttcgc gcggcgtgga aggcaaaatc
     5161 gccgctgtgc gctatgcccg cgaaaacaac gtgccatact tgggcatctg cctcggtatg
     5221 cagattgcgc tgatcgaata cgcccgcgac gtggcaggtt tgaaaggtgc gaattccacc
     5281 gagttcgacc tcaaatgcgc cgcccccgtc gttgccctga ttgacgaatg gcaaactgcc
     5341 gacggcagcg tcgaaacccg cgacgaatcc gccgatttgg gcggcaccat gcgtttgggc
     5401 gcgcaagaag tcgaattgaa agcaggcagc ctcgccgcca aaatctacgg cagcgaacac
     5461 atccgcgaac gccaccgcca ccgctacgaa gtcaacaaca actacgttcc tacgctggaa
     5521 caggcaggtt tggtcatcgg cggcgtatcc gccggacgcg aacgcttggt cgaaaccatc
     5581 gaactgccga accatccttg gttcttcgcc tgccagttcc accccgagtt tacctccaat
     5641 ccgcgcaaag ggcatccttt gttcaccgcg tttgtcaaag ctgcgttgaa caataaaaaa
     5701 gcctgataaa gcgttacttg atgatcaaaa tgccgtctga aagcctgaat gaagactttc
     5761 agacggcatt tttgcaaatc ggggatcata aaagccatca atttaatttt tatggtttta
     5821 tctaatattg accaactgaa tattgttaag cggggcgtga aactcaagtt gagacctttg
     5881 caaaattccc caaaatcccc taaattccca ccaagacatt taggagattt c
//
LOCUS       DAXHPG010000129         5911 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXHPG010000129 DAXHPG010000000
VERSION     DAXHPG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.129"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            102..1007
                     /gene="glyQ"
                     /locus_tag="ACFM6F_001677"
     CDS             102..1007
                     /gene="glyQ"
                     /locus_tag="ACFM6F_001677"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC6165060.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1084..1494
                     /locus_tag="ACFM6F_001678"
     CDS             1084..1494
                     /locus_tag="ACFM6F_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding protein YggL"
                     /protein_id="HFC6165061.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFDRFMDELIAYLAANGLEMAGGGDCGDFSCLFQKSGSRGLGETEKKAIISWLA
                     GREDVATLHATPLVDGFYGDDGWENYSETYKQAD"
     gene            1515..3578
                     /gene="glyS"
                     /locus_tag="ACFM6F_001679"
     CDS             1515..3578
                     /gene="glyS"
                     /locus_tag="ACFM6F_001679"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC6165062.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARRLNATVAADEALLDEVTALVEYPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTADPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDNLPNGKVA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3595..3996
                     /locus_tag="ACFM6F_001680"
     CDS             3595..3996
                     /locus_tag="ACFM6F_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6165063.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNAEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKWDSRIKILAHDRLLNRKQQRRDSPANTFKHR
                     LIRALTKISREREKRRQRREQLIGKIIVPFQ"
     gene            4008..4940
                     /locus_tag="ACFM6F_001681"
     CDS             4008..4940
                     /locus_tag="ACFM6F_001681"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFC6165064.1"
                     /translation="MDIVFAADDNYAAYLCVAAKSVEAAHPDTEIRFHVLDAGISEAN
                     RAAVAANLRGGGNIRFIDVNPEDFAGFPLNIRHISITTYARLKLGEYIADCDKVLYLD
                     IDVLVRDSLTPLWDTDLGDNWLGACIDLFVERQEGYKQKIGMADGEYYFNAGVLLINL
                     KKWRRHDIFKMSCEWVEQYKDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFMAN
                     RFASRHTDPLYRDRTNTVMPVAVSHYCGPAKPWHRDCTAWGAERFTELAGSLTTVPEE
                     WRGKLAVPHRMFSTKRMLQRWRRKLSARFLRKIY"
     gene            4995..>5911
                     /locus_tag="ACFM6F_001682"
     CDS             4995..>5911
                     /locus_tag="ACFM6F_001682"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6165065.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVVNQTWCNLDILIVDDGSTD
                     GTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSGGEYIARTDADDIAAPD
                     WIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHGKIWKKPTRHEDIADFFP
                     FGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSKLGRLAYYPEALVKYRLH
                     ANQVSSKYSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQIKAVAYELLEKHLP
                     EEDFERARRFLYQCFKRTDTLPAGAWLDFA"
BASE COUNT         1517 a         1636 c         1542 g         1216 t
ORIGIN      
        1 atgtatgccg tgttttcaaa tcagtctgtc tttgagataa acctatccaa acagcaatgg
       61 aaacagtaaa ataaaccctt tattttatcg aatcataagc catgcttacc ttccaacaaa
      121 tcatcttcaa actgcaaaca ttctgggcag acaaaggctg caccgtcatc caacccttcg
      181 acatggaagt cggtgccggc acatcccatc ccgccacctg cctgcgcgca ctcggccccg
      241 agccttggtt tgccgcctac gtccaaccca gccgccgccc caaagacggc cgctacggcg
      301 acaaccccaa ccgcctgcaa cactattacc aattccaagt cgccctcaag cccgctcccg
      361 ccaatatcca agacctctat ctcgactctc tgcgcgaatt gggcatcgat cccaaagtcc
      421 acgacatccg ctttgtcgaa gacgactggg aaaaccccac cctcggcgcg tggggtttgg
      481 gctgggaagt ctggctcaac ggcatggaag taacccagtt tacctatttc caacaagtcg
      541 gcggcatcga ctgcaccccc gtactcggcg aaatcaccta cggcatcgaa cgcttggcga
      601 tgtacctgca aggcgtggaa aacgtctatg acctcgtttg ggcaaaaacg ctggacggta
      661 atacagtaac ttacggcgac gtgtaccacc aaaacgaagt cgaacaatcc acctacaact
      721 tcgaatacag cgatgccgac tggctgctgc gccagttcaa cgactacgaa gcgcaagcca
      781 aacgcctgct tgccgaagaa aacgccgccc tcgccctgcc tgcctacgag cttgtcctca
      841 aagcggggca tacgttcaac cttttagacg cacgcggcgc gatttccgta actgagcggg
      901 caacttatat tggacgtatt cgtgcattga gccgcgccgt ggcgcaaaaa tatgttgaaa
      961 gccgcgaaaa actgggcttc cctttaatga aggcaaacgc cgcctaaggg ggcgacgatt
     1021 ttttacgttc gggcggcacg ataaggcagc cgtccggaag ccattatatt ttttaggaaa
     1081 atcatgcctg tctatttccg caccccaagc aggaaacgcc tgcacaaaat gaaccgccgc
     1141 cagcataaaa agctgcattt gggcgagttt caaacattga ttttcggcgt tcaaggcagt
     1201 ttgcaccaag ctgaggacga tgccgaagcg ttcgaccgct ttatggacga gctgattgcg
     1261 tatcttgcgg caaacggctt ggaaatggcg ggcgggggcg attgcggcga tttttcgtgc
     1321 ctgtttcaaa aatccggctc ccgaggcttg ggcgaaaccg agaaaaaagc cattatctct
     1381 tggctggcag gtcgcgaaga tgtcgccact ttgcacgcca ccccgctggt tgacggtttt
     1441 tacggggatg acggctggga aaattacagc gaaacctata aacaggcaga ttaaccaaat
     1501 tcaaagaaag ccgaatgatg acccaaaccc ttttaatcga actccttacc gaagaactcc
     1561 cgcccaaagc tctgaataat ctgggcaacc attttgccgc cgccgttgcc gaaggcttgg
     1621 aaaaagcgca actggttgac ggtgcagccg aatttacggc ttatgcctcg ccgcgccgtt
     1681 tggcggttca agtcaaaaac gttaaagccg ttcaggccga ccaaaaaatc gtgaaaaaag
     1741 gccctgccgt ggcgaatgcc atgaaagacg gtgcgccgac caaggctttg gaaggtttcg
     1801 cacgcggtgc gggcgcgaaa atcgaagact tgaccatcgt ccacgacggc aaacaggatg
     1861 tgtacgccta cgaatacgtc caaaccggca aaccgctggg cgaacttttg gaagacatta
     1921 tcaatcaagc cgttaagaaa ctgccgattc cgaaagtgat gcgttggggc agcagcacgt
     1981 ttaccttcgt gcgccctgtt cacgggctga ttgtgctgca cggcggcgac atcgtaaacg
     2041 tcagcgtttt gggtctgcaa agcagcaatc aaaccttggg acaccgcttc ttgtccaacg
     2101 gcgaaatcac cattgaaaac gccgacagct acgccgaaca aatgcgcggg caaggcaaag
     2161 tcgtcgcttc gtttgccgag cgcaaagccg cgattcagac ggcattggaa gggcaggcgc
     2221 gccgtctgaa cgcgaccgtt gccgccgatg aagcattgct ggacgaagta accgcgctgg
     2281 tcgaataccc cgttgttttg gaagccggtt ttgaggaaca cttcctcgcc gtgcctcaag
     2341 aatgcttgat tttgacgatg cagcaaaacc aaaaatactt cccgctgctc gaccaaaacg
     2401 gcaagctgat gaaccgcttc ctgctggtct ccaacctgca aaccgcagac ccgtcgcaca
     2461 tcatccaagg caacgaacgc gttttgcgcg cgcgcctgtc tgatgccgag ttcttctaca
     2521 aacaagacca aaaggcgact ttggaaagcc gcctgcccaa gctggcaaac gtggtttatc
     2581 acaacaaaat cggttcgcaa gccgaacgca tcgaacgcct gcaaagcatt gccgcccaca
     2641 tcgctaaagc gttgggcgca gatgccgccg ccgccgaacg cgccgcgcgt ctggcaaaag
     2701 ccgacttggt aaccgaaatg gtcggcgagt tccctgaatt gcaaggcacc atgggcaaat
     2761 actatgcccg tttggacggc gaaaccgaag aaatcgccga agccatcgag caacactacc
     2821 aaccgcgttt cgccggcgac aatctgccga atggcaaagt tgccaccgcc gtggcactgg
     2881 cggacaaact agaaaccttg gtcggcattt ggggcattgg tctgattccg accggcgaca
     2941 aagaccccta cgccctgcgc cgcgccgcct tgggtatttt gcggatgctg atgcagtacg
     3001 gtttggacgt gaacgaactg attcagacgg cattcgacag cttccccaaa ggtttgctca
     3061 acgaaaaaac gccgtctgaa accgccgact ttatgcaggc gcgccttgcc gtgttgctgc
     3121 aaaacgatta tccgcaagac atcgttgccg ccgtattggc gaaacagccg cgccgtttgg
     3181 acgacgtagt ggcgaaactg caagccgttg ccgcgttcaa acaactgccc gaagccgccg
     3241 cgctcgccgc cgccaacaaa cgcgtgcaaa atctgctgaa aaaagccgat gccgagttgg
     3301 gcgcggttaa cgaaagcctg ctgcaacagg acgaagaaaa agccctcttt gccgccgcgc
     3361 aaggcttgca gccgaaaatc gccgccgccg tcgccgaagg caatttccaa accgccttgt
     3421 ccgaactggc ttccgtcaag ccgcaagtcg atgccttctt tgacggcgtg atggtgatgg
     3481 cggaagatgc cgccgtaaaa caaaaccgtt tgaacctgtt gaaccgtttg gcagagcaga
     3541 tgaacgcggt agccgacatc gcgcttttgg gcgagtaacc gttgtacagt ccaaatgccg
     3601 tctgaagcct tcagacggca tcgtgcctat cgggagaata aattgcagcc tttagtcagc
     3661 gtattgattt gcgcctacaa cgcagaaaaa tatttcgccc aatcattagc cgccgtcgtg
     3721 aatcagactt ggcgcaactt ggagattttg attgtcgatg acggctcgac agacggcaca
     3781 cttgccattg ccaaggattt tcaaaagtgg gacagccgta tcaaaatcct tgcacacgac
     3841 cgcctcctga accgcaaaca gcaaaggcgc gattcccccg ccaacacatt caaacaccgc
     3901 ctgatccgcg ccttgaccaa aatcagcagg gaaagggaaa aacgtcggca aaggcgcgaa
     3961 cagttaatcg gcaagatcat tgtgcctttc caataaaagg agaaaagatg gacatcgtat
     4021 ttgcggcaga cgacaactat gccgcctatc tttgcgttgc ggcaaaaagc gtggaagcgg
     4081 cccatcccga tacggaaatc aggttccacg tcctcgatgc cggcatcagt gaggcaaacc
     4141 gggcggcggt tgccgccaat ttgcgggggg ggggtaatat ccgctttata gacgtaaacc
     4201 ccgaagattt cgccggcttc cccttaaaca tcaggcacat ttccattacg acttatgccc
     4261 gcctgaaatt gggcgaatac attgccgatt gcgataaagt cctgtatctg gatatagacg
     4321 tattggtcag ggacagcctg acgcccttat gggataccga tttgggcgat aactggcttg
     4381 gcgcgtgcat tgatttgttt gtcgaaaggc aggaaggcta caaacaaaaa atcggtatgg
     4441 cggacggcga atattatttc aatgccggcg tattgctgat caacctgaaa aagtggcggc
     4501 ggcacgatat tttcaaaatg tcctgcgaat gggtggaaca atacaaggac gtgatgcaat
     4561 atcaggatca ggacattttg aacgggctgt ttaaaggcgg ggtgtgttat gcgaacagcc
     4621 gtttcaactt tatgccgacc aattatgcct ttatggcgaa caggtttgcg tcccgccata
     4681 ccgacccgct ttaccgcgac cgtaccaata cggtgatgcc cgtcgccgtc agccattatt
     4741 gcggcccggc aaagccgtgg cacagggact gcaccgcgtg gggtgcggaa cgtttcacag
     4801 agttggcggg cagcctgacg accgttcccg aagaatggcg cggcaaactt gccgtcccgc
     4861 accgtatgtt ttcgacaaag cgtatgcttc aaagatggcg cagaaagctg tctgccagat
     4921 tcttacgcaa gatttattga cggggcaggc cgtctgaagc cttcagacgg catcgtgcct
     4981 atcgggagaa taaattgcag cctttagtca gcgtattgat ttgcgcctac aacgtagaaa
     5041 aatatttcgc ccaatcatta gccgccgtcg tgaatcaaac ttggtgcaac ttggatattt
     5101 tgattgtcga tgacggctcg acagacggta cgcttgccat tgccaaggat tttcaaaagc
     5161 gggacagccg tatcaaaatc cttgcacaag ctcaaaattc cggcctgatt ccctctttaa
     5221 acatcgggct ggacgaattg gcaaagtcgg ggggggaata tattgcacgc accgatgccg
     5281 acgatattgc cgcccccgac tggattgaga aaatcgtggg cgagatggaa aaagaccgca
     5341 gcatcatcgc gatgggcgcg tggctggaag tgttgtcgga agaaaaggac ggcaaccggc
     5401 tggcgcggca tcacaggcac ggcaaaattt ggaaaaagcc gacccggcac gaagatattg
     5461 ccgacttttt ccctttcggc aaccccatac acaacaacac gatgattatg cggcgcagtg
     5521 tcattgacgg cggtttgcgt tacaacaccg agcgggattg ggcggaagat taccaatttt
     5581 ggtacgatgt cagtaaattg ggcaggctgg cttattatcc cgaagccttg gtcaaatacc
     5641 gccttcacgc caatcaggtt tcatccaaat acagcgtccg ccaacacgaa atcgcgcagg
     5701 gcatccaaaa aaccgccaga aacgattttt tgcagtctat gggttttaaa acccggttcg
     5761 acagccttga ataccgccaa ataaaagcag tagcgtatga attgctggag aaacatttgc
     5821 cggaagaaga ttttgaacgc gcccgccggt ttttgtacca atgcttcaaa cggacggaca
     5881 cgctgcccgc cggcgcgtgg ctggattttg c
//
LOCUS       DAXHPG010000130         5880 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXHPG010000130 DAXHPG010000000
VERSION     DAXHPG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.130"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(147..1190)
                     /gene="argC"
                     /locus_tag="ACFM6F_001683"
     CDS             complement(147..1190)
                     /gene="argC"
                     /locus_tag="ACFM6F_001683"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC6165066.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLDAIPLLP"
     gene            complement(1187..1468)
                     /locus_tag="ACFM6F_001684"
     CDS             complement(1187..1468)
                     /locus_tag="ACFM6F_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165067.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSTANQNPVGQRSDGIPANHTKEKP"
     gene            1836..2990
                     /locus_tag="ACFM6F_001685"
     CDS             1836..2990
                     /locus_tag="ACFM6F_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6165068.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAARNLEAASWKDINEI
                     YQRADPTAPLFYLRKKGAIVPKEEYVESFDALGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHISRQVARFAGAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHLA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKHEEVRKLIYQLIELDFLELL"
     gene            2999..3205
                     /locus_tag="ACFM6F_001686"
     CDS             2999..3205
                     /locus_tag="ACFM6F_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165069.1"
                     /translation="MKNWKQFTFFVILVIACYQVLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQ"
     gene            3410..3670
                     /locus_tag="ACFM6F_001687"
     CDS             3410..3670
                     /locus_tag="ACFM6F_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6165070.1"
                     /translation="MERKNNIIELSIENLELIYGGGLGERILEGGANGAQIGGSIGAG
                     IGLGVGIVSGGIPAGPAIILGGTVGAAVGAVTGMGVAAYEYY"
     gene            3830..4456
                     /locus_tag="ACFM6F_001688"
     CDS             3830..4456
                     /locus_tag="ACFM6F_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HFC6165071.1"
                     /translation="MNRPKQPFFRPEVTVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITTKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRAANLFIL"
     gene            4360..4608
                     /locus_tag="ACFM6F_001689"
                     /pseudo
     CDS             4360..4608
                     /locus_tag="ACFM6F_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFP transporter"
     gene            4921..5223
                     /locus_tag="ACFM6F_001690"
     CDS             4921..5223
                     /locus_tag="ACFM6F_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165072.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            5507..5659
                     /locus_tag="ACFM6F_001691"
                     /pseudo
     CDS             5507..5659
                     /locus_tag="ACFM6F_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
BASE COUNT         1584 a         1259 c         1431 g         1606 t
ORIGIN      
        1 attcccgctt ttgcgggaat gacgggattt tagatttttg ttttcgcgga ataatgagcc
       61 aaaagccgtc tgaacctaaa aaaccgccac tttcagatgg catcaaaaaa ccgcaggcac
      121 gatacctgcg gtttgccgtt tgcgtttcag gggagcaggg ggattgcgtc caagccgtgt
      181 gtttccttca gtccgaacat gatgttcata ttttgcaccg cctgacccgc cgcgcctttg
      241 acgaggttgt cgatgacgga aaggacgacc cacacatcgg attgcgccgc ctgttggatg
      301 ctgatgcggc agaggtttgc gccgcgcacg ctgcgggttt cgggtgtcga accggcgggc
      361 aggatgtcca cgaacaggct gtcgcggtaa tagtcgcgca ggatggtttc ggggcagatg
      421 ccgtctgaaa ggtggaggta aacggtggcg tgcataccgc gtatcatcgg cgcgaggtgc
      481 ggcgtgaaca cgaatccttc ggcgatgccg tcctgaagcc cggcgatggt ctgcctgatt
      541 tcgggcaggt ggcggtgtcc agctatgccg taggctttga agttgtcgcc gacttcgcac
      601 aacagcgaac cgacattgcc tttcctgccc gcgccggaca cgccggattt gcagtcggca
      661 atcagcggca taccgggctt cagacggcat tgccgcaaca gcggcacgag cggcagggat
      721 acgcaggtcg ggtagcagcc ggggttggcg acgaggcgtg cctgtgcgac ggcttcgcgg
      781 ttgagttcgc tcaatccgta cacggcttgg gaaacgaggt cgggggcggc gtgggtcatg
      841 ccgtaccagt gttcccaggt cggaatgtcc cgtatgcgga agtcggcgga aaggtcgatg
      901 acgcgcacgc cctgttcaat caggcgcggc gcgtctttca tggcgatgcc gttgggcgtg
      961 gcgaagaaga cgatgtcgca ttgttccaaa cctgcctcgt cgggcgtttg gaaggcgagg
     1021 ccgtacacgc cgcgcaaact cggaaagtaa tcggcaactg cagttcccgc ttcgctgcgg
     1081 ctggttacgg cggcgacttc gacatcggga tgggcggcaa gcaggcgcag cagttccacg
     1141 cccgtgtagc ccgtcgcgcc gacaatgccg actttgattt ttttactcat ggtttttcct
     1201 ttgtgtggtt ggcgggtatg ccgtccgaac gctgtccgac ggggttttga ttggcggtgg
     1261 aaggacggta cacaaagcgt ttgaaaagcg tgtctaattt aatggcatcc gcctctgttt
     1321 ttaaaaccca gccctgccgt ccggaataaa cataaccgta ggcgcgaaag tcggcaggat
     1381 atttcggtgc ctgccccaaa cccaaaccgt atgcggcgaa agcggtggtc gtggagaagg
     1441 aaagcaggag gaggacgagg gttttcataa ggttccaaac cggacggggc gatgaagccc
     1501 gattatagca aaaggtcgtc tgaaaaccgt tttcagacga ccttttattt aattacaata
     1561 aaaatgttaa caacaaaaaa caaaccgctt tttttcgttt ggaaacaaaa caaattttta
     1621 tagtggatta aatttaaacc agtacagcgt tgcctcgcct tgccttgccg tactatctgt
     1681 actgtctgcg gtttcgtcgc cttttcctga tttttgttaa tccactataa ctaaaattgt
     1741 caacaattcc ttgacgcaca cacaaaaaaa actttaatat ttgtaaatat atgtaacatt
     1801 ataaatttta aatattttct gagtaagggg aagtaatgag catccatctc gacttcggca
     1861 ttagtcctaa aacgttccga cagacttatc tgtatcaaaa gcccaagctc tttaaaggag
     1921 cggctcggaa tctcgaagcc gcatcttgga aagatatcaa cgagatatac caacgagcag
     1981 acccaaccgc accgctgttt tatctgcgta aaaaaggtgc aattgttcct aaagaagaat
     2041 acgtcgaaag tttcgacgct ttgggcaaaa ctcgctaccg ttttattaaa tccgttatct
     2101 acgaacatat gaagaatggt gcgtcgttag tctataacca tattaacaac gagccgtttt
     2161 cagatcatat ttcccgtcaa gtcgcccgct ttgctggcgc acatactatt gtcagtggat
     2221 atcttgcttt tggcagcgac gaatcttata aaaaccattg ggacacccgc gatgtgtatg
     2281 ccatccagct tttcggcaag aaacgttggc aacttactgc ccctgatttc cctatgccat
     2341 tgtatatgca acagactaaa gatactgata tttccattcc tgaacatatc gatatggata
     2401 ttatccttga agcaggtgat gtcctctaca tcccgcgcgg ttggtggcac agacctatcc
     2461 cgctcggctg tgaaaccttc cacctcgctg tcggtacctt tccgcccaac ggctataatt
     2521 acctcgagtg gctaatgaag aaattcccca cgatagaaag tctgcgccac agtttctcag
     2581 actgggagca agataggacg cgtatcaacg atactgccgc acaaattgct gccatgattg
     2641 ccgaccccgt caattacgaa gccttcagtg aagacttcct cggcaaagaa cgcaccgata
     2701 ccgcttttca tctcgaacag ttcgcgaatc ccaacgctac tccgctttca gacgacgtca
     2761 ggttgagact aaatgccaat aatttggata cgttggaaaa ggggtatttg attgggaatg
     2821 ggatgaagat aagcgtagat gaattgggga aaaaagtttt agaacacatc ggtaagaatg
     2881 aaccgttatt gttgaaaaat ctactggtta acttcaatca gggaaaacat gaagaagtta
     2941 ggaagttgat ttatcagttg atagagttag attttctgga acttttgtga gggattctat
     3001 gaaaaactgg aagcaattta catttttcgt aatattagta atagcttgtt atcaagtgct
     3061 atatttttta tcagatatgt ttcttctcga ctacattaac aaatatagtt ggaatttgaa
     3121 ttttattcag ggtactttga attttttttc aatatatctg ccatatgttt ttgttagccg
     3181 aatttttagg aatactaacc aatgaaagac taggctatga aatcaaaatt tatgtataag
     3241 ttactggtac ttgcaaagta cctgtatatt ttaatttgta tatcaatacc aatcgttttt
     3301 atttatgaaa gctaccaagg agatgatgtt gattttatga tttatattca tgcttttatt
     3361 attttttcca tattgggtgt attgactcaa tattctacgt ctaatttttt tggaaaggaa
     3421 aaataacatc attgaactat ccattgagaa tctggagcta atttatggcg gaggcttagg
     3481 ggagagaatc cttgaaggag gagcaaatgg tgcacaaata ggaggatcaa ttggtgcagg
     3541 tattggtctg ggtgtcggta tagtttctgg tggtattcct gccggaccgg ctattattct
     3601 cggtggcaca gttggtgctg ctgttggtgc tgtaactggt atgggtgttg cagcatatga
     3661 gtattactaa gaaactcagc ccaaagggac agtaacggtc ggtccgattt ctgcttttgc
     3721 tgtggacaaa tcaggaaacg gctacactga cggtacagat tactgctgat atttctcatc
     3781 tttcagacga tctctagggg tcgtctgaaa ctttcttaac ttcaatttta tgaatagacc
     3841 caagcaacct ttcttccgcc ctgaagtcac agttgcccgg caaaccagcc tgacgggtaa
     3901 agtgattctg acacgaccgt tgtcattttc cctatggacg acatttgcat cgatatctgc
     3961 gttactgatt atcctgtttt tgatatttgg taactatacg cgaaagacaa cagtggaggg
     4021 acaaatttta cctgcatcgg gcgtaatcag ggtgtatgca ccggatacgg ggacaattac
     4081 aacgaaattc gtggaagatg gagaaaaggt caaggctggc gacaagctat ttgcgctttc
     4141 gacctcacgt ttcggcgcag gaggtagcgt gcagcagcag ttgaaaacgg aggcagtttt
     4201 gaagaaaacg ttggcagaac aggaactggg tcgtctgaag ctgatacacg agaatgaaac
     4261 gcgcagcctt aaagcaactg tcgaacgttt ggaaaaccag aaactccata ttttgcaaca
     4321 gatagacggt cagaaaaggc gcattagact tgcggaagat cagtggcaaa aacggcactg
     4381 ggcagacagg aattgtcggg attgggcatg gtatcctccg atttggcgaa gagcagcgaa
     4441 cctgtttatc ttgtgaaaat aaaacccgac aaatcaacca tcactgcata cggtgaggaa
     4501 aaaccgctgc aaatcggcat gacgttggaa gcagacatcc tgcacgagaa acggcgattg
     4561 tacgaatggg tattggagct gatttatagt atatcgggca aactgtaaga cttgaggctg
     4621 aaagataaag aagttgtctg aaaccttttc agacgaccag aagacaatat ataaaaacac
     4681 aaacttcaaa actgcctgaa accatcctct tttacaggca ctccgtctgt aaaagcaagg
     4741 atagggcatc tgcccttttg attgtttgat taacgataca aggagtttca aaatgagagt
     4801 tttatagtgg attaacaaaa accagtacag cgttgcctcg ccttgccgta ccatttgtac
     4861 tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat ccactatatg tgttcatgaa
     4921 atgacttggg tcggaggctc aggtaatgcg caacaaagtt catattattg cgaaatttgc
     4981 gaatctgcag ggcttaacga tacgggaaat cctgataaat ctttaggatt gccaaacaat
     5041 acgttcagta atccgcctgg ttggggagct acaatcggag ctttagcagg tagccgcata
     5101 gggatgcctg aatttggtac gtttgcgagc catgccattg aaaatttcga ctggtcatgg
     5161 tatcgacgtt atagggaaat tgccgaaacg attgaacgag aatattcagg cggtttgcct
     5221 taatagttga ggaggtcatg atgtttgcca aacattatca attcatcgca ctcggcatca
     5281 tgctgcttct ttatatgttg attctctata cgaccgattt ttccaatctg acgtattgga
     5341 tgctgttttt tatctgtttt attacaggaa aaatattagc tcgtttgtta gagaaaagct
     5401 ttaaataaaa tagcagctag tcgcaaaagg tcgtctgaaa ccttttcagg cggcctttct
     5461 aaaatacatc caacttccta atccctattt ttcaaaaagg aaatctatgc cccatctgca
     5521 aaacctgtct ttgggcttaa agaaaaagct gcctgttatc ctgcaaacag aaatatcaga
     5581 atgcggcttg gcatgtctgg cggctgtggc gggatttcat ggtttccata cgaatttacg
     5641 cgcactgcgt tcaaaatact gtccgagacc tttgcaaaat tccccaaaat cccctaaatg
     5701 tcttggtggg aattttgggg aattttgcaa aggtctcatt ctataactgt aaatactttt
     5761 aaatttatga caaaatagta aatattgcta aaataatatt gatgtcatga aattttttcc
     5821 tgctccatgt ctgttggtta tcctggctgt catacccctt aaaaccttag ctgccgatga
//
LOCUS       DAXHPG010000131         5870 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXHPG010000131 DAXHPG010000000
VERSION     DAXHPG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.131"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            45..458
                     /locus_tag="ACFM6F_001692"
     CDS             45..458
                     /locus_tag="ACFM6F_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC6165073.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            504..1133
                     /gene="nth"
                     /locus_tag="ACFM6F_001693"
     CDS             504..1133
                     /gene="nth"
                     /locus_tag="ACFM6F_001693"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC6165074.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            1271..2770
                     /locus_tag="ACFM6F_001694"
     CDS             1271..2770
                     /locus_tag="ACFM6F_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC6165075.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGKEITVAVKLGN
                     ASEQTGSSSEPDKAPYAEHQSGTFSVESAGITLQTHTDSSGGRLVVVRVSGAAERAGL
                     RRGDEILAVGQVPVNDEDGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            2973..4493
                     /locus_tag="ACFM6F_001695"
     CDS             2973..4493
                     /locus_tag="ACFM6F_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC6165076.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            4550..5635
                     /gene="nagZ"
                     /locus_tag="ACFM6F_001696"
     CDS             4550..5635
                     /gene="nagZ"
                     /locus_tag="ACFM6F_001696"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC6165077.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEERQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIESFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQEAQAFVAGLASPQDTAGGVKVGEAF"
     gene            5716..5792
                     /locus_tag="ACFM6F_001697"
     tRNA            5716..5792
                     /locus_tag="ACFM6F_001697"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5750..5752,aa:Pro,seq:cgg)
BASE COUNT         1327 a         1806 c         1489 g         1248 t
ORIGIN      
        1 cgtacaatcc gtcgccgatt tttgcctgtt tgagactgtt tgcaatgatg cccgcctacg
       61 cctacgccgc gctcgccttt tccgccttca cttccgccac cctcctgccc ggcacatccg
      121 aagccgcctt tgccctgttc gtccgccgct ttcccgaaca cgcatacggc gcgttgctgt
      181 gcgccggcct tgccaacgga ttgggcagta tggtttccta ttggatggga cgtttgctgc
      241 cttcccgaaa aatgccgtct gaaaaaacac tgaatctgat gcggcgtttc ggtatttggc
      301 tgcttccgtt tgcctggctg cccgtcgtcg gcgacgcact gccgcttgcc gccggctggc
      361 tgcggttgaa tccgtggaca agcgggctga tgctggttat cggcaaaacg gcgcgttacg
      421 ccttcattct gtggggaatg caatattacg ccgcctgaac cgtgcgttat aatgccgacc
      481 gcacaaaccg actgcacaaa aaaatgaaca gacacatccg ccaagaaatt ttcgaacgct
      541 tccgcgccgc caacccccat ccgaccaccg agctgaattt caactcccct ttcgagcttt
      601 taattgccgt tctgctttcg gcgcaggcga ccgatgtcgg cgtaaacaag gcgacggcga
      661 agctgtttcc ggttgccgat acgccgcagg cgatgctgga tttgggtttg gacggcgtga
      721 tggaatacac gaaaaccatc gggctgtata aaaccaagtc caagcacatc atgcaaacct
      781 gccgcatcct gctggaaaaa tacaacggcg aagtgccgga agaccgcgag gctttggaat
      841 cgttgcctgg cgtggggcgc aaaacggcaa acgtggtatt gaacacagcg ttcggacacc
      901 ccgttatggc ggtcgatacg catattttcc gcgtgtccaa ccgaaccaaa atcgcccccg
      961 ggaaagatgt gcgcgaagtc gaagacaaac tgatgcgctt cattcctaaa gaatttctga
     1021 tggacgcgca ccactggctg attttgcacg gacgctacac ctgcaaggca ctcaaaccgc
     1081 aatgccaaac ctgcatcatc aacgatttgt gcgaatatcc cgccaaagcc tgaccgccgc
     1141 cgcccttcaa aatgaaacgc ttgaaaattc ggacggacgt tgtagaattt caacctatgc
     1201 cgtctgaagc cgacaaaatg cgcttcagac ggcattaaag ccggtaccgg catccgacaa
     1261 aggacaaaac gtgttcaaaa aataccaata cctcgctttg gcagcactgt gtgccgcctc
     1321 gctggcaggc tgcgacaaag ccggcagctt tttcggtgcg gacaaaaaag aagcatcttt
     1381 cgtagaacgc atcgaacaca ccaaagacga cggcagcgtc agtatgctgc tgcccgactt
     1441 tgtccaactg gttcaaagcg aaggcccggc agtcgtcaat attcaggcag cccccgcccc
     1501 gcgcacccaa aacggcagcg gcaatgccga aaccgattcc gacccgcttg ccgacagcga
     1561 cccgttctac gaatttttca aacgcctcgt cccgaatatg cccgaaatcc cccaagaaga
     1621 agcagatgac ggcggattga acttcggttc gggcttcatc atcagcaaag acggctatat
     1681 tctgaccaat acgcacgtcg ttaccggcat gggcagtatc aaagtcctgc tcaacgacaa
     1741 gcgcgaatat accgccaaac tcatcggttc ggatgtccaa tccgatgtcg cccttctgaa
     1801 aatcgacgca acggaagagc tgcccgtcgt caaaatcggc aatcccaaag atttgaaacc
     1861 gggcgaatgg gtcgccgcca tcggcgcgcc cttcggcttc gacaacagcg tgaccgccgg
     1921 catcgtgtcc gccaaaggca gaagcctgcc caacgaaagc tacacaccct tcatccaaac
     1981 cgacgttgcc atcaatccgg gcaactccgg cggcccgctg ttcaacctga aaggacaggt
     2041 cgtcggcatc aactcgcaaa tatacagccg cagcggcgga ttcatgggca tttccttcgc
     2101 catcccgatt gacgttgcca tgaatgtcgc cgaacagctg aaaaacaccg gcaaagtcca
     2161 acgcggacaa ctgggcgtga ttattcaaga agtatcctac ggtttggcac aatcgttcgg
     2221 tttggacaaa gccggcggcg cactgattgc caaaatcctg cccggcagcc ccgcagaacg
     2281 tgccggcctg caggcgggcg acatcgtcct cagcctcgac ggcggagaaa tacgttcttc
     2341 cggcgacctt cccgttatgg tcggcgccat tacgccggga aaagaagtca gcctcggcgt
     2401 atggcgcaaa ggtaaggaaa tcaccgttgc cgtcaaactg ggcaatgctt ccgaacaaac
     2461 cggttcctcg tccgagccgg acaaagcccc ttatgccgaa caccaatccg gtacgttctc
     2521 ggtcgaatcc gcaggcatta cccttcagac acataccgac agcagcggcg gacggcttgt
     2581 cgtcgtgcgg gtttcggggg cggcagaacg cgcaggcttg aggcgcggcg acgaaatcct
     2641 tgccgtcggg caagtccccg tcaatgacga agacggtttc cgcaaagcta tggacaaggc
     2701 aggcaaaaac gtccccctgc tggtcatgcg ccgtggcaac acgctgttca tcgcattaaa
     2761 cctgcaataa cacacataat ttaaccaaaa tattttttaa atgataaaat gcccgtcaat
     2821 accgattaac gggcatttta cagaaatcag ttggttgatt ttaaacaaat attttttacg
     2881 acaaaaaata tttccgccca ttttgccaac aaaacccaaa ccggaggcgg acagatacgc
     2941 tcccatccgg acgcttaaca cccccgtaaa ccatgcagct gatcgactat tcacattcat
     3001 ttttctcggt tgtgccaccc tttttggcac tggcacttgc cgtcattacc cgccgcgtac
     3061 tgctgtcttt aggcatcggt attctggtcg gcgttgcctt tttggtcggc ggcaaccccg
     3121 tcgacggtct gacacacctg aaagacatgg tcgtcggctt ggcttggtca gacggcgatt
     3181 ggtcgctggg caaaccaaaa atcttggttt tcctgatact tttgggtatt tttacttccc
     3241 tgctgaccta ctccggcagc aatcaggcgt ttgccgactg ggcaaaacgg cacattaaaa
     3301 accggcgcgg cgcgaaaatg ctgaccgcct gcctcgtgtt cgtaaccttt atcgacgact
     3361 atttccacag tctcgccgtc ggtgcgattg cccgccccgt taccgacaag tttaaagttt
     3421 cccgcgccaa actcgcctac atcctcgact ccactgccgc gcctatgtgc gtgctgatgc
     3481 ccgtttcaag ctggggcgcg tcgattatcg ccacgcttgc cggactgctc gttacctaca
     3541 aaatcaccga atacacgccg atggggacgt ttgtcgccat gagcctgatg aactattacg
     3601 cactgtttgc cctgattatg gtgttcgtcg tcgcatggtt ctccttcgac atcggctcga
     3661 tggcgcgttt cgaacaagcc gcgttgaacg aagcccacga tgaaactgcc gcttcagacg
     3721 ctaccaaagg tcgtgtttac gcactgatta ttcccgtttt ggccttaatc gcctcaacgg
     3781 tttccgccat gatctacacc ggtgcacagg caagcgaaac cttcagcatt ttgggtgcat
     3841 ttgaaaatac ggacgtgaac acttcgctgg tattcggcgg cacttgcggc gtgcttgccg
     3901 tcgtcctctg cacgctcggc acgattaaaa ccgccgatta tcccaaagcc gtttggcagg
     3961 gtgcgaaatc catgttcggc gcaatcgcca ttttaatcct tgcctggctc atcagtacgg
     4021 ttgtcggcga aatgcacacg ggcgactacc tctccacgct ggttgcgggc aacatccatc
     4081 ccggcttcct gcccgtcatc cttttcctgc tcgccagcgt gatggcgttt gccacaggca
     4141 caagctgggg gacgttcggc atcatgctgc cgattgccgc cgccatggcg gtcaaagtcg
     4201 aacccgcgct gattatcccg tgtatgtccg cagtaatggc gggggcggta tgcggcgacc
     4261 actgctcgcc catttccgac acgaccatcc tgtcgtccac cggcgcgcgc tgcaaccaca
     4321 tcgaccacgt tacctcgcaa ctgccttacg ccttaaccgt tgccgccgcc gccgcatcgg
     4381 gctacctcgc attgggtctg acaaaatccg cgctgttggg ctttggcacg acaggcattg
     4441 tattggcggt gctgattttt ctgttgaaag ataaaaaacg cgccaacgcc tgaccccttt
     4501 ccctgttcag acggcatatg ccgtctgaag ctttttggag caaaccgcaa tgaccgtccc
     4561 ccatattccg cgcggccccg tcatggcaga cattgccgcc ttccgcctga ccggagaaga
     4621 aaggcaacgc ctgctcgacc ccgcaatcgg cggcatcatc ctcttccgcc gcaacttcca
     4681 aaacatcgaa caactcaaaa cactcaccgc cgaaatcaaa gccctgcgca cacccgaact
     4741 cattatcgcc gtcgatcacg aaggcggcag ggtgcaacgt ttcatcgaaa gcttcacccg
     4801 cctgcccgca atgagtacgc tcggcgagat ttgggacaaa gacggcgcgt ccgccgccga
     4861 aaccgccgcc ggacaagtcg gccgggtttt ggcaaccgag ctttccgcct gcggcatcga
     4921 tttgtccttc acgcccgtct tagatttgga ctggggaaac tgccccgtca tcggcaaccg
     4981 cagtttccac cacaatcccg aagcggtcgc ccacctcgcc ctcgcccttc aaaaaggttt
     5041 ggcaaaaggc ggtatgaaat cctgcggcaa acattttccc ggacacggat ttgtcgaagg
     5101 agacagccat ctggtcttgc cggaagacag gcgcagcctg tccgaactcg aagccgccga
     5161 cctcgccccc ttccacatta tgagccgcga aggtatggcg gcggttatgc ccgcccacgt
     5221 cgtttatcca caagtggaca caaagcccgc agggttttcc gaaatctggc tcaaacaaat
     5281 tttgcgccgc gacatcgggt ttaaaggcgt aattttctcg gatgatttga ctatggaggg
     5341 cgcgtgcggg gcaggcggca tcaaagaacg cgcccgcatt tcctttgagg caggttgcga
     5401 catcgtcctc gtctgcaacc gtcccgacct tgtggacgaa ctgcgcgaag atttccgcat
     5461 tcctgacaat cccgctttgg cgcaacgttg gcaatacatg gccaacacgc tcggcagtgc
     5521 cgccgcgcaa gccgttatgc agacggcgga tttccaagag gcgcaagcct ttgttgccgg
     5581 ccttgcctcg ccgcaagaca cggcgggcgg cgtaaaagtc ggcgaagcct tttaaatccg
     5641 cccgacacgc tgcaaaaagg caaaacggta taggcacgcc gtattttttg cgctataatc
     5701 acgccttact taattcggaa tgtagcgcag cccggtagcg cacttcgttc gggacgaagg
     5761 ggtcggaggt tcgaatcctc tcattccgac caaatccaaa ccgcctcggc atccattgcc
     5821 ggagcggttt ttccttttgg cggcaacaga aaaatgccgt ctgaaacaga
//
LOCUS       DAXHPG010000132         5849 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXHPG010000132 DAXHPG010000000
VERSION     DAXHPG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.132"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(99..1016)
                     /gene="thrB"
                     /locus_tag="ACFM6F_001698"
     CDS             complement(99..1016)
                     /gene="thrB"
                     /locus_tag="ACFM6F_001698"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC6165078.1"
                     /translation="MSVYTSVSDDEMRGFLDDYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLG"
     gene            complement(1052..1780)
                     /gene="ubiG"
                     /locus_tag="ACFM6F_001699"
     CDS             complement(1052..1780)
                     /gene="ubiG"
                     /locus_tag="ACFM6F_001699"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC6165079.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1932..3173
                     /locus_tag="ACFM6F_001700"
     CDS             1932..3173
                     /locus_tag="ACFM6F_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC6165080.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRSWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3306..3869
                     /gene="gmhB"
                     /locus_tag="ACFM6F_001701"
     CDS             3306..3869
                     /gene="gmhB"
                     /locus_tag="ACFM6F_001701"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC6165081.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            3899..4642
                     /locus_tag="ACFM6F_001702"
     CDS             3899..4642
                     /locus_tag="ACFM6F_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6165082.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETV"
     gene            4639..5328
                     /locus_tag="ACFM6F_001703"
     CDS             4639..5328
                     /locus_tag="ACFM6F_001703"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6165083.1"
                     /translation="MTSLIHTLSDGMELTIEIKRRAKKNLIIRPIGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRRTLAKTPPQTAENRLPESILFHGRQLALTAHQDTQILLMPS
                     EIRVPEGAPEKQLALLQDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQWL
                     KIHGRELFALG"
     gene            5440..5625
                     /locus_tag="ACFM6F_001704"
     CDS             5440..5625
                     /locus_tag="ACFM6F_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165084.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
BASE COUNT         1371 a         1898 c         1383 g         1197 t
ORIGIN      
        1 gaaaatactc ggctatcgtc tcattttcgt tttaatcagc cataaaaatg ccgtctgaaa
       61 ccctttcaga cggcatttct gtcaaacgcc ggacgcactc aacccaaact caacagcagg
      121 ttgcggaacg cgttcgggtc tttgataaac gtcatctcgc ccgcctgcgg aaaatgaaaa
      181 tccaacaggc gcgacaccca aaaacggatg cagcctgcac gttgggcggt cgggaaatac
      241 gccttttctt cggcactcaa ggggcgcacg ccctcataac cgccgataaa cgcctttttc
      301 aacgcctcat ccaacttatt gtccgccgtc cttgcccaat cgttgaccgc aatcgccaag
      361 tcatacataa aattgccccg gcaggcataa tagaaatcga tgaaacccga tacctgaccg
      421 ccgtcaagca acacattgtc tttaaacaga tcggcatgga tgatgcccga aggcagatga
      481 ttgccgagat tgtccttcaa cgcatcgatt tcggaacaca gcagtgcggc atcgtcttgc
      541 gacaggacgg gcagcagccg ggcgcacgcc tccgtccacc acgcatcgta acgcgggttt
      601 tccatttcca aagggaaatc agcggcggca aggtgcattt tggccaacat cgcaccggta
      661 tggaagcact gctccgccgt cggcagcgca gtatccgaac ctttcaggca ggcaaccagg
      721 caggcaggct tgcccgccaa aacggaatca agccggccgt ctttgcgcgc aaccggcgcg
      781 gcaaccgcca cgcccttcat actcaaatgc cggttaagct ccagaaaaaa cggcagctct
      841 tcctgtttca acacttcaaa cacggtcagc acataacgtc ccgaagtcgt cgtcagaaaa
      901 taattgctgt tggtaatccc ctgcgcgatg ccctgcaggg aaacaaattc tcccaaatcg
      961 taatcatcca aaaagccgcg catttcatca tcggaaacac tggtataaac agacatatcc
     1021 attcacccgt tcaaaacaat caaacccgcc gtcagaacgc cggacggcag gcaaacatat
     1081 aattcacatc ggtcgaatcg cacagggcat aagtttgcga caacacatgg taagtcatac
     1141 ccttcgtatc cgccacatcc aagcctgcct gacggcacat ccgcgccagc tcggcaggcg
     1201 cgatgaattt tttccagtcg tgcgtacctt tggggacgaa cttcaacaga tattccgccg
     1261 ccacaatcag atgcaggtac gatttcgggt ttttattgat ggtggaaaaa aacaccatgc
     1321 cgtccggttt gaccagcttg gcacaggcgc gaacgatggc ggcgggatcg gggacgtgtt
     1381 ccatcatttc catgcacgtt accacatcga acgagtgcgg ttccgcctcg gcaaggtctt
     1441 ccacgcggat acattcgtat tcgatatcgg cgacattgtt caaagccgcg tgcaggcggg
     1501 cggtttccag cgactgctcc gccatgtcga taccctttac aaacgccgcg ccgcgccgcg
     1561 ccatactttc cgccaagatg ccgccgccgc agcccacatc caaaacccgt ttgccgcgca
     1621 aatccgcgtg tccgtcgata taatccagcc gcagcggatt gatgtcgtgc aaggttttga
     1681 actcgcccga cttgtcccac catttgtcgg caatccggct gaatttggcg atttccccct
     1741 catcgacatt atattttttg tcggacattt tccctcccat ctgacgaacc gcccactcca
     1801 aaacccaaga ttatactatg gaacgcaatg ccgtctgaaa gccttttcgg gcggcgcggc
     1861 gcaaatacct tacaaatcct tacactttac ggcataatgg cggctcgctt tttctggcag
     1921 aaagacaaaa tatgcccacc aaaacccctt cactgttcgg cggcgcgatg attatcgccg
     1981 gcacggtcat cggcgcaggt atgctcgcca acccgaccgc cacatccggc gtatggttta
     2041 ccggctcgct ggccgtgttg ctgtacacct ggttttccat gctctccagc ggcctgatga
     2101 ttttggaagt caacacccac tacccccacg gcgcgagctt cgacaccatg gttaaagacc
     2161 tgctcggacg gagctggaac atcatcaacg gcatcgccgt cgccttcgtt ttatacctgc
     2221 ttacttacgc ttatatcttc gtcggcggcg acctgaccgc caaaggctta ggcagcgcgg
     2281 caggcggcaa tgtttcactc accgtcggac aactcgtctt cttcggcatt ctcgcctttt
     2341 gcgtatgggc atccgcacgc ttggtcgacc gattcaccag cgtcctcatc ggcggcatgg
     2401 tattaacctt tatttgggca accggcggcc tgattgccga tgccaaactg cccgtcctct
     2461 tcgacaccca agcccctacc ggcaccaact actggattta tgtcgccacc gccctgcccg
     2521 tctgccttgc gtcattcggt ttccacggca acgtctccag cctgctcaaa tactttaaag
     2581 gcgacgcgcc caaagtggct aaatccatct ggacgggcac actgattgcg ctggtaattt
     2641 acgtcctctg gcaaaccgcc atccaaggca acctgccgcg caacgagttc gcccccgtga
     2701 ttgccgccga agggcaagtc tccgtcctga ttgaaaccct gtccaaattc gcccaaaccg
     2761 gcaatatgga caaaatattg tccctgtttt cctatatggc gatcgccacc tcgtttttag
     2821 gcgtaacgct cggactcttc gactacatcg ccgacatctt caaatggaac gacagcgtgt
     2881 ccggccgcac caaaaccgcc gcgctgacct tcctgccgcc tctaatttcc tgcctgctct
     2941 tccccaccgg ctttgttacc gccatcggct acgtcggcct ggcggcaacc gtctggacag
     3001 gcatcatccc cgccatgctg ctctaccgtt cgcgcaaaaa attcggcgca ggcaaaacct
     3061 ataaagttta cggcggcttg tggctgatgg tttgggtctt ccttttcggc atcgtcaaca
     3121 tcgccgcaca ggtattgagc caaatggaac tcgtccccgt atttaaagga taaaggcaaa
     3181 atgccgtctg aagcccgccg gcggcttcag acggcattgc ctcgttatca cgcggcacag
     3241 gcgcatcact gccgatattt cagtataatg ccacccccga cctgccccaa tccaaaggaa
     3301 acgcgatgaa actcatcatt ctcgaccgcg acggcgtcat caatcaggac cgcgatgact
     3361 tcgtcaaatc cgttgacgag tggatacctg tcgaaggcag catggatgcg gtggcatttc
     3421 tgacgcaggc aggctacacc gtcgccgttg ccaccaacca atccggcatc gggcgcaaat
     3481 attttaccgt tcaaaacctt accgaaatgc acgccaaaat gcaccgcctc gtccgtcagg
     3541 caggcggcga aatcaacggc atctggttct gcccgcacac cgatgccgac aactgcaact
     3601 gccgcaagcc caaaccgggt atgattgaag acatcatcgg acgcttcaac gcccaagcct
     3661 cggaaacctg gctggtcggc gacagcctgc gcgatttgca ggcaatcgat gccgtcggcg
     3721 gaaaacccgc gctggttctg accggaaaag gcaaaaaaac gctctcccaa cacggacacg
     3781 aattacccga acacacacag gttttcgata ccttgctcga tttctcacaa tacatcatgc
     3841 aggaaaacgc cgcaccgcaa gccgactgaa cataccgcat ttccgacaag gcaaaaccat
     3901 gctcatcatc cgcaacctga tttactggct gatactctgt tccaccctga ttttcctctt
     3961 tccctttatg ctgctcgcct cgcctttccg ggacggggcg cacaagatgg cgcgggtctg
     4021 ggtcaaaatc ctcaacctct cgctcaaaca catcgtcggg ctcaaatacc gcatcatcgg
     4081 cgcggaaaac atccccgacc gccccgccgt catctgcgcc aaacaccaaa gcggctggga
     4141 aacgcttgcc cttcaggaca tttttccgcc gcaggtttac gttgccaaac gcgagctgtt
     4201 caaaatcccc tttttcggct ggggtttaaa actggtcaaa accataggca tagaccgcaa
     4261 caaccgccgc gaagccaacg aacagctcat aaaacagggg ttggcgcgca aaaacgaagg
     4321 ctattggatt accattttcc ccgaaggcac gcgccttgcg cccggaaaac gcggcaaata
     4381 caaactcggc ggcgcgcgca tggcgaaaat gtttgagatg gacatcgtcc ccgtcgccct
     4441 caacagcggc gaattttggc cgaaaaactc ctttctgaaa tatccggggg aaatcaccgt
     4501 cgtcatctgt ccgaccatcc cgcacgcaag cggcagcgaa gccgaattga tggaaaaatg
     4561 cgaacacctc atcgaaacgc agcagccgct catttccggc gcaggcccgt ttgccgccaa
     4621 aatgccgtct gaaaccgtat gacctccctg atccacactc tttctgacgg catggaactg
     4681 actatagaaa tcaagcgccg tgccaagaaa aacctgatta tccgccctat cggcacacat
     4741 accgtccgca tcagcgtccc accctgcttc tccgtctccg ctctaaaccg ctggctgtat
     4801 gaaaacgaag ccgtcctgcg gcgaacactg gcgaaaacac cgccgcaaac tgccgaaaac
     4861 cggctgcccg aatccatcct cttccacggc agacaacttg ccctcaccgc ccatcaagac
     4921 acgcaaatcc tgctgatgcc gtctgaaatc cgcgttcccg aaggcgcacc cgaaaaacag
     4981 cttgcgctgc tgcaggactt tttggaacgg caggcgcaca gttacctgat tccccgcctc
     5041 gaacgccacg cccgcaccac acaactgttc cccgcctcct cctcgctgac ctctgccaaa
     5101 accttctggg gcgtgtgccg caaaaccaca ggcatacgcc tcaaccggcg gctggtcggc
     5161 gcaccggaat acgttgccga ttatgtctgc atacacgaac tctgccacct cgcccatccc
     5221 gaccacagcc ccgccttttg ggaactgacc cgccgcttcg ccccctacac gcccgaagcg
     5281 aaacagtggc tcaaaatcca cggcagggaa cttttcgcct taggctgacg cggtcccggg
     5341 ccggcatccg ccaattcaga cctcctccca aacggacatc cggaaagcag catcacagcc
     5401 ggcgacacat accgctgccg ccgctttcag acggcatccg tgccggaaca ggcacgcgcc
     5461 cgcccgattc aaaccgcgat gacgctttgc cgccggttcg gggcaggatg gcggcacacg
     5521 cgccgtctgc cgcgtttcat ttcacaccgc tcttccgaaa cccgaaaccc gcccggtccg
     5581 acgtgcggta tgaaacgctt aagctggcgc gaagtctttt actgatttgc ccgcgaaaat
     5641 gccgtctgaa aggttttcgg acggcatttt tttgcgtttc ccaggatggc ggcggattcg
     5701 taaaaggcgg tcagggtgga ttgtaggatg ggttgagacc tgccgaatcc gccgcatctg
     5761 ccaaatctac cgccgtcatt cctacgaaag tgggaatcta gaacgcgggg ttaagaaaac
     5821 ctgcatcccg tcattcccac gaaagtggg
//
LOCUS       DAXHPG010000133         5729 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXHPG010000133 DAXHPG010000000
VERSION     DAXHPG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.133"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            225..5675
                     /gene="iga"
                     /locus_tag="ACFM6F_001705"
     CDS             225..5675
                     /gene="iga"
                     /locus_tag="ACFM6F_001705"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC6165085.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPKKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGNGKHHWNITSG
                     TNSHIGSTAVRLANNERDANNGQNVTFEDNGTLVLDQNINQGAGGLFFKGDYTVKGAN
                     NDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEVNGTGVNQGQLKVGDGTVI
                     LNQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFE
                     HIRNVDEGARIVNHNTDHASTITLTGKSLITDPKTISIHYIQNNDDDDNGYYYYRPRK
                     PIPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWVFMGYKQEEAQKNAMNHKN
                     NQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLS
                     GRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNV
                     SNITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKALNSFGATQINGN
                     VNLSQNAALVLGKAALWGQIQGQGNSSVSLNQHSKWHLTGDSQVHNLSLADSHIHLNN
                     ASDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGE
                     PNQEGLNLFDASSVQDRSHLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRV
                     KPAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKR
                     QQAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQ
                     KVEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQ
                     SQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELVRRQQEERKAAEL
                     LAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQA
                     LAARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAE
                     LARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQD
                     YMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDE
                     NVSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELH
                     EKVETVSLQPRAAQPRAQAAAQPQAQAVAQADAVSTNTNSALSDAMASTQSILLDTGA
                     SLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQ
                     IGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQK
                     ANFNRASIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTL
                     TAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFAYKADNQQKYSAGAALL
                     YRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1931 a         1366 c         1282 g         1150 t
ORIGIN      
        1 acaaaaagcc gcagaaattt aacggaagaa ccgaaccccg aaaatcggct tcccaccttc
       61 gcctgaataa cagaacaaag gaagcggaaa taaaaacatc gggattcttt ttttaaattg
      121 gcaataaaac accaaaatga ataattcaca caacaaaaaa tagtcaacac cgccaaatcg
      181 gcgtaaaata caagacatta tcacccttaa aacggtaaaa ccttatgaaa accaaacgtt
      241 ttaaaattaa cgccatatcc ttatccatct ttcttgccta tgcccttacg ccatactcag
      301 aagcggcatt ggtcagagac gatgtcgatt atcaaatatt ccgtgacttt gcagaaaaca
      361 aaggcaaatt ttttgtcggc gcaaccgatt tatcagtgaa aaacaaacaa ggtcaaaaca
      421 tcggcaacgc attatccaac gtaccgatga ttgattttag cgttgcagat gtcaacagac
      481 gtacattaac cgtcatcgat ccccagtatg ccgtcagcgt caaacatgta aaaggagacg
      541 aaatctctta ttacgggcat cacaatgggc acttagatgt tagcaatgat gaaaacgaat
      601 atcgttcagt tgcacaaaat gactacgaac caaataaaaa ttggcatcac ggtaatcaag
      661 gtcgtcttga agactacaac atggcacgcc ttaataaatt cgtaacagaa gtcgcaccta
      721 ttgcaccaac cagtgcaggc ggcggcgttg aaacctataa agataaaaac cgtttttctg
      781 agtttgtgcg agttggagca ggtacgcaat ttgaatataa cagccgctac aatatgacag
      841 agctttcacg agcttatcgt tatgccattg caggtacgcc ttatcaagac gtgaatgtca
      901 catcaaatct aaatcaagaa ggcttgattg gcttcggtga taattcaaaa catcattccc
      961 caaaaaaact caaagaagtt ttatctcaaa acgcattgac taactatgct gtgttaggcg
     1021 atagcggttc accattattt gcttatgata aacaagaaaa acgctgggtc tttttaggtg
     1081 cttacgatta ttgggcaggc tatcaaaaaa actcttggca agaatggaat atctataaaa
     1141 aagaatttgc agataaaatc aaacaacgcg ataacgccgg caccatcaaa ggtaatggaa
     1201 aacatcattg gaacattact tctggcacaa acagccatat cggttcgaca gcggtaaggc
     1261 ttgccaacaa tgaaagggat gcaaacaacg gacagaatgt tacctttgaa gacaacggca
     1321 ctttggtatt ggatcaaaac atcaaccaag gcgcgggcgg tctgtttttc aaaggcgatt
     1381 acacagtcaa aggtgcaaat aatgacatca cttggctagg tgcggggatt gatgttgccg
     1441 acggcaaaaa agtcgtttgg caagtcaaaa atccgaatgg cgacagattg gcaaaaatcg
     1501 gcaaaggcac tttggaagta aacggcacag gcgttaacca agggcaatta aaagtcggcg
     1561 acggtacggt tattctgaat caacaagccg atgccgacaa aaaagtccag gctttctccc
     1621 aagtcggcat tgtcagcgga cgcggtacat tggtattaaa tagttcaaat cagattaatc
     1681 ccgataacct atatttcggt ttccgtggcg gtcgtttgga tgccaatggc aatgacttga
     1741 cttttgaaca catccgcaac gtggatgaag gtgcgcgcat tgtcaaccac aacacagacc
     1801 atgcctccac aatcacgcta acgggtaaat ctttgattac cgatcccaaa accatctcta
     1861 ttcattatat tcaaaataat gatgatgacg ataatggtta ttactattac cgccctagga
     1921 aaccgattcc acaaggcaaa gatctttatt acaaaaacta tcgttattac gccctaaaat
     1981 ccggcggcag cgtgaacgca ccgatgcccg aaaacggaca aacggaaaat aacgactggg
     2041 tctttatggg atataagcaa gaagaagccc agaaaaacgc gatgaaccac aaaaacaatc
     2101 agcgtattag cggttttagc ggtttctttg gcgaagaaaa cggaaaagga cataacggcg
     2161 cattaaacct taatttcaac ggcaaaagcg cgcaaaaccg tttcttgtta acaggcggca
     2221 cgaatttaaa cggaaaaata agcgtaactc aaggcaatgt cttgttatca ggtcgtccaa
     2281 caccacacgc aagagatttt gtgaacaaat cttcagcccg aaaagacgca catttctcca
     2341 aaaacaatga agtcgtattt gaagacgact ggataaaccg cacattcaaa gccacagaaa
     2401 ttgcggttaa tcaatccgca tcattctctt ccggcagaaa tgtatccaac attaccgcca
     2461 acatcaccgc aacagacaac gccaaagtaa atttgggtta caaaaacggc gatgaagttt
     2521 gcgtgcgctc ggactatacc ggttacgtta cctgcaacac agacaactta tccgataagg
     2581 ctttaaacag ctttggagcg acacagatta acggcaatgt gaatttgagt cagaatgcgg
     2641 cattggtctt aggcaaggct gcattatggg ggcaaattca agggcaagga aacagcagcg
     2701 tcagcctaaa ccaacatagc aaatggcatt tgaccggcga cagccaagta cacaatctgt
     2761 cattggcgga tagccatatt catttgaaca acgcttccga cgcgcaaagt gcaaataaat
     2821 accacacgct caaaatcaat catttatccg gtaacgggca ttttcattat ctgacgcacc
     2881 tggcgaaaaa tcttggggat aaggtgcttg tgaaggaatc cgcatccggt cattatcagc
     2941 tccatgttca agataaaaca ggcgaaccca atcaggaagg gctgaatctc tttgatgcat
     3001 catccgtaca agaccgttcg cacctttctg tttccttggc gaataaccat gtagatttag
     3061 gcgcattgcg ttatacaatc aaaacagaaa acggtattac ccgattgtac aatccttatg
     3121 ccgagaaccg ccgccgagtc aaaccggccc cgtctcctgc cacaaacacg gcttctcaag
     3181 cacaaaaggc aacacaaacg gacggtgcac aaattgccaa gcctcaaaat atcgtcgtcg
     3241 caccacctag cccgcaggca aatcaagccg aagaagcaaa acgccaacaa gcaaaagcgg
     3301 agcaagtgaa gcgtcagcaa gcagaagcag agcgtaaatc ggcagagtta gcaaaacaaa
     3361 aagcagaagc cgaacgcgaa gcaagagagc tggcgacccg ccaaaaagca gaacaagaac
     3421 gcagcagcgc cgaacttgca cgtcgacatg aaaaagaacg cgaagcggca gagttgtcgg
     3481 caaaacaaaa agtcgaagcc gaacgtgagg ctcaagcatt ggcggtacgc cgaaaagccg
     3541 aagccgaaga ggcaaaacgc caagctgcag agcttgcacg ccggcatgaa aaagaacgcg
     3601 aagcggcaga gttgtcggca aaacaaaggg tcggggaaga ggaacgccgt caaacagccc
     3661 aatcccagcc gcaacgccgc aaacgccgtg ccgcaccgca ggattatatg gcagcttccc
     3721 aagaccgtcc taaacgccgc ggacaccgtt cggtacagca aaacaatgtg gaaattgccc
     3781 aagcccaagc agaacttgtg cgccgacagc aggaagaacg caaagcggca gagttgttgg
     3841 caaaacaaag ggcagaagcc gaacgtgagg ctcaagcatt ggctgcgcgc cgaaaagcag
     3901 aagccgaaga ggcaaaacgc caagctgccg aacttgcaca tcgccaggaa gcagaacgca
     3961 aagcggcaga attgtcggca aaccaaaaag ccgcagctga agcccaagca ttggcggcac
     4021 gccaacaaaa agcccttgcg cgtcaacagg aagaagcacg aaaagctgca gagttagccg
     4081 tcaaacaaaa agcggaaacc gaacgcaaaa ccgccgaact tgccaagcaa agggcagcag
     4141 ccgaagcagc aaaacgccag caagaagctc gccagaccgc cgaacttgca cgtcgtcaag
     4201 aagcggaacg ccaagcagca gagttgtcgg caaaacaaaa agccgaaaca gatcgagaag
     4261 cggcagaatc ggcgaaacga aaagctgagg aagaagagca ccgtcaagca gcccaatccc
     4321 agccgcaacg ccgaaaacgc cgtgccgcac ctcaggatta tatggcagct tcccaaaatc
     4381 gtccgaaacg tcgcggacgc agatctactc tgccggcacc gccctcgcca tcatttgatt
     4441 cgtcagctta cgcagcaccc agggccttgc ataatccgga ctggtatgaa aatgactatg
     4501 aagaaatccc cttggatgcg ttggaagatg aaaatgtatc tgaatcagtt gacacatcag
     4561 acaaacagcc tcaagacaat acggaacttc atgaaaaata tgaaaatgat tatgaagaaa
     4621 tccccttgga tgcgttggaa gatgaagatg tatccgaatc ggttgacaca tcagacaaac
     4681 agcctcaaga caatacggaa cttcatgaaa aagttgagac agtgagtttg caaccaagag
     4741 ccgcgcagcc gcgagcccaa gccgccgcgc agccgcaagc ccaagccgtc gcgcaagccg
     4801 atgcagtcag caccaatacc aactcggctt tatctgacgc aatggcaagc acgcaatcta
     4861 tcttgttgga tacaggtgct tcattaacac ggcacattgc acaaaaatca cgtgctgatg
     4921 ccgaaaaaaa cagcgtttgg atgtcgaaca tcggttatgg ccgtgattat gcttccgcac
     4981 aatatcgccg gtttagttcg aaacgcacgc aaacacaaat cggcattgac cgcagcttgt
     5041 ccgaaaatat gcagataggc ggcgtattga cttactctga cagtcagcat acttttgatc
     5101 aggcgagcgg caaaaatact tttgtgcaag ccaaccttta tggtaagtat tatttaaatg
     5161 atgcttggta tgtggccggc gatattggtg caggcagctt gagaagccgg ttacaaacgc
     5221 agcaaaaagc aaactttaac cgagcaagca tccaaaccgg ccttactttg ggcaatacgc
     5281 tgaaaatcaa tcaattcgag attgtcccca gtgcggggat ccgttacagc cgcctgtcat
     5341 ctgcagatta caagttgggt aacgacagtg ttaaagtaag ttctatgtca gtgaaaacac
     5401 tgacggccgg actggatttt gcttatcggt ttaaagtcgg caaccttacc gtaaaaccct
     5461 tgttatctgc agcttacttt gccaattatg gcaaaggcgg cgtgaatgtg ggcggtaatt
     5521 ccttcgccta taaagcagat aatcaacaga aatattcagc aggtgccgcg ttactgtacc
     5581 gtaatgttac attaaacgta aatggcagta ttacaaaagg aaaacaattg gaaaaacaaa
     5641 aatccggaca aattaaaata cagattcgtt tctaaaatac taaattcata gcaaaataaa
     5701 atgccgtctg aactcaggct tcagacggc
//
LOCUS       DAXHPG010000134         5691 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXHPG010000134 DAXHPG010000000
VERSION     DAXHPG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.134"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            98..814
                     /locus_tag="ACFM6F_001706"
     CDS             98..814
                     /locus_tag="ACFM6F_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC6165086.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            963..1865
                     /locus_tag="ACFM6F_001707"
     CDS             963..1865
                     /locus_tag="ACFM6F_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC6165087.1"
                     /translation="MDSLMTLISVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGTNLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGLSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            1908..2681
                     /locus_tag="ACFM6F_001708"
     CDS             1908..2681
                     /locus_tag="ACFM6F_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC6165088.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2793..4481
                     /locus_tag="ACFM6F_001709"
     CDS             2793..4481
                     /locus_tag="ACFM6F_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC6165089.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4574..5530
                     /gene="gshB"
                     /locus_tag="ACFM6F_001710"
     CDS             4574..5530
                     /gene="gshB"
                     /locus_tag="ACFM6F_001710"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC6165090.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELGGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1332 a         1540 c         1570 g         1249 t
ORIGIN      
        1 aatttgtgca atgctttaat tcctctgaca cgatttgaaa cacagccgcc ttaaaatatc
       61 ttaaaaaatt tgaaagcgta aaaatacgga aaacatcatg aactttgaaa acgacgacat
      121 tatccatgcg ccgactacgt cttccctgat tctcgaagag cggcacgatt ctgagctgtt
      181 ccgtgtttac gccctgattt tggacggcat taccgatcag gtgctgctgc ccggcaaaaa
      241 gctgaccgaa tccgaacttt gccgtcagat ggtgtgttcg cgcaacaccg tccgcggcgc
      301 gctgtcgctt ttggcgcacg acaagattgt cgatttgcaa cccaacaggg gcgcgttcgt
      361 ccacgttccc gatttgaaag aaatgcagga tgtgttcaat gcgcgcatcg aaatggagac
      421 gatgattttg aatatcctcg cagatttgcc ggatttggaa acgcgcctca agccgcttta
      481 tgcgatgata cggcgcgaag aagaggcctc cggcaggggc gaccgcgttg gctggaaccg
      541 cctgtccaat gccttccacg tcgaactggc gcgcctggtg ggcaacgatg tgctgttcga
      601 cattatgaac acgctgtgcg cgcggtcttc cctgattgtc gccgtggcgg gcgtgcatcg
      661 cgaggaaaaa cacgccatca atacgcatac gcattcagaa caccgcgaaa tcctcgacct
      721 gctgctggcg ggcaggcgca accgcgtggt caaaatcctg cgccgccatt tgggcaactg
      781 tatggagcgt ttggaaaaga ctttggaaga ttgaatgccg gagcggataa gccgcctgaa
      841 ccttcaggcg gttttttaat ggcgaacctg atgccgtctg aaatatggat gcgggtatct
      901 gcaattttca gacggcaatt tttaaaccgc acatatcatg cggcaataaa ggaggccagg
      961 agatggacag cctgatgaca ttgatttcgg ttttgatacc gatgtttgcc ggatttttta
     1021 tccgtgtgcc caagccttac ctgcccgctt tggataaggt gctatcggtt ttggtgtatg
     1081 ccgtgctgct gctgatcggc gtatcgttgt cgcgcgtgga ggatttgggt tcgcggttgg
     1141 acgatatggc gttgacggtt ctgtggctgt ttgtttgtac ggtcgggacg aacctgcttg
     1201 ctttggcagt gttgggaaag ttattcccgt ggcggataaa ggggaaaggg aagggcgttt
     1261 cggtcggtgt gtcgggcagt gtggggcagc tcggatgcgt gctgctcgga tttgcatccg
     1321 gcaaactgat gcgcgatatt tggatgccgt ctgaaagcgc gggcatgtat tgtctgatgc
     1381 tgctggtgtt cctcatcggc gtacagctca aaagcagcgg cctatcgttg cggcaggttt
     1441 tggtcaaccg caggggtatt cggttgtcgg tctggtttat gctttcatct ctttcgggcg
     1501 ggctgctgtt tgccgcatcg gcagacggtg tgtcgtggac gaaaggtttg gcgatggctt
     1561 ccggcttcgg ttggtattcc ctctcgggtt tggtcatgac cgaggcttac ggcgcggtat
     1621 ggggcagcat catgctgctg aacgatttgg cacgagagct gtttgcactg gcatttatcc
     1681 cgctgctgat gaagcgtttt ccggatgcgg cggtgggggt tggcggtgcg accagtatgg
     1741 attttacatt gcccgtgatt cagggtgcgg gcggtttgga agtcgtgccg gtagcggtca
     1801 gcttcggcgt ggtggtcaat attgccgccc cgtttctgat ggtggtgttt tccacgctgg
     1861 gctgaacgcg gtaaaatcgg catcccgatg caaggaggca gaaaacgatg aaaccgaaaa
     1921 tccaaaggca cggagagatt ttaagccttg tccgccggca tcagtttatg tcggtggacg
     1981 agcttgccgc cgcattggac gttaccccgc agacgatacg ccgcgacatc cgcgaattgg
     2041 aggatgctgg cagcctgaaa cgccaccacg gcggcgcatc ctcgggcgga aacttgccgg
     2101 aggggctgcc tgccagccgc caaattcaat gtcaaaacga aaaaaacgcc attgcccggc
     2161 tgattgcgga acatattccc gacgagtcat cgctgtttgt cagtatcggc acgacgatgg
     2221 aagccgtggc atcggagctg gtgagacggc gcggcagcct gcggattatt accaacaata
     2281 tccacgtcgc ctccatcgtt tcggcgcgga cggattacac ggtcatcatc acttccggag
     2341 tcgtccgccc tttggacggc ggcattaccg gcgtggcaac cgtcgatttt atcaaccagt
     2401 tcaaagtcga ttatgccgtg atgagtacgc acggcgtgga aagcgacggt tcgcttttgg
     2461 acgacgacta caaagaagtc agcgtgatgc aggcgatgat tgccaacgcg cgcgtccgct
     2521 tcctcggcgt ggaccacagt aaattccgca gcaacgcgct ggtcaggctc ggcgacatta
     2581 cggcgtttga caaagtattt accgaccggc tgcccgatac cgcgatgcag aagatgctga
     2641 aagaggcggg ggtggaatgc ctgattgccg atgccgtctg aacgctatgt caaagcgcgc
     2701 aagtcgggta caataaacac atcatcaaac cgcttcagac ggcataagga accgccccaa
     2761 tgccgtctga agccatctgt tcaaagaaaa ctatgctcaa taaagaccaa tttgcggaca
     2821 accattttat ccgcaccttc atcgaagaag acctcaaaag cggcaaacac gaaaccatcc
     2881 aaacccgttt tccgccggag cccaacggct acctgcacat cggacacgcc aaatccatct
     2941 gcctgaactt cggtttggcg tatatttacg acggcttgtg caacctgcgt tttgacgata
     3001 ccaaccccga aaaagaaaac gacgaatacg tcaacgccat caaagaagac gttgagtggc
     3061 tcggtttcca ttgggcgggc gagccgcgtt tcgcttccaa ctatttcgac cagctttatg
     3121 actacgccgt cggtttggtt aaagacggca aagcgtatgt cgatgatttg acgccagaag
     3181 aaatgcgcga ataccgcggt acgctgaccg aagcaggcaa aaacagccct tatcgcgacc
     3241 gcagtatcga agaaaacctc gacctgttca cgcgcatgaa aaacggtgag ttccccgacg
     3301 gcagcaaaac cctgcgcctg aaaatcgaca tggcatccgg caacatcaat atgcgcgatc
     3361 cggtcatcta ccgcatccgc cgcgcccatc accacaacac cggcgacaaa tggtgcatct
     3421 atccgatgta cgactacacg cattgtattt ccgatgccat cgaaggcatc acgcattcct
     3481 tgtgtacgct cgaatttgaa gcccatcgtc cgctgtatga ctgggtgttg gacaacatcc
     3541 ccgcgccgca cgccacacgt ccgcgccaat acgagttttc acgcttggag cttttgtaca
     3601 ccattacctc caaacggaaa ttgaatcagt tggttgtgga aaaacacgtt tccggctggg
     3661 acgatccgcg tatgccgacc atttccggta tgcgccgacg cggctacacg cccgaagggc
     3721 tgcgcctgtt tgccaaacgc gccggtattt ccaaatctga aaacatcgtc gatatgagcg
     3781 tgttggaagg cgcgattcgc gaagagttgg aaaattccgc cccacgcctg atggcggtgt
     3841 tgaacccgct caaagtgacc ctgaccaact ttgaaaccgg cagaacccaa agccgccgtg
     3901 ccgcgttcca tccgaaccac gaggaaatgg gcgagcgcga agtacctatt tcacaaacca
     3961 tctatattga agccgacgac tttgccgaaa atccgcccaa aggcttcaaa cgcctgattc
     4021 ccggcggcga agtacgcttg cgccacggct atgtcatcaa gtgcgatgaa gtcgtcaaag
     4081 acgaagcagg caatgtggtt gaactcaaat gcagcatcga ccacgacacc ttgggcaaaa
     4141 atccagaagg ccgtaaagtt aaaggcgtga ttcattgggt ttccgccgaa cacgccgccg
     4201 aaatcaaagt ccgtctgtac gaccgcctct ttaccgtcga gcgtcccgat gccgtgcgcg
     4261 gcgaagacgg cgaatacctg ccgttcaccg atttcctcaa tccggaatcc gtcaaggaaa
     4321 tcaccgccta cgccgaacct gccgccaaag atttgccggc agaaagccgt tggcagttcg
     4381 agcgtatcgg ctacttcgta accgaccgta aagaccacgg caaagacacg ccggtgttta
     4441 accgcacggt cacgctgaaa gattcttggc agcctaagta aaaccccatc cttgccgtct
     4501 gaatattgtt cggacggcat ttcccctttg cccgcgaaat gcggcacatt cggcacaccg
     4561 gaaaggaaat atgatgaaag tcctctttat cgccgacccg atggcaagtt tcaaaaccta
     4621 caaagacacc acctacgcca tgatgcgcga gatggcaaaa cgcggttggc ggctgtttca
     4681 taccttgagc ggggaattgt cggttaaaaa cggtctggtg gttgctgatg cgtctgcttt
     4741 tgagtttctc ggcgcacaag acgaccacga caaaaactgg tttgccgccg ccgacaaagt
     4801 tcagacggca ttgaaagcat ttgatgccgt gattatgcgt accgatccgc cgttcgatat
     4861 gcaatacctc tacgccaccc aattactgac gctggcggag cagcagggcg cgaaggtctt
     4921 taacagcgga caggcgatgc gtgatttcaa tgaaaaactg gcgattttga acttcagccg
     4981 ctttaccgcg cccacgctgg taacgacccg ttccgccgat gtccgcgcat ttttgaaaga
     5041 acacggcgac atcatcatta aaccgctcga cggcatgggc ggtatgggca tcttccgcct
     5101 gaccgaaaaa gaccccaaca tcggcagcat cctcgaaacc ctgatgcagc ttgattcccg
     5161 caccattatg gcgcaacgct acattcccga aatcgtacac ggtgacaaac gcatcctgat
     5221 tatcggcggc gaagtcgtcc cctatgcttt ggcgcgtatc ccgcaaaacg gcgaaacacg
     5281 cggcaacctt gcggcaggcg ggcgcggcgt ggcgcaggaa ttgggcggac gcgaccgcga
     5341 aattgccgaa acgctcgccc ccgaactcaa acggcgcggc atcctgctgg ccggtttgga
     5401 cgttatcggc agcaacctga ccgaagtcaa cgtaaccagc ccgaccggat tccaagaaat
     5461 tatgaaacaa aaaggtttcg acgtggcggc aatgtttgcc gatgccgttg ccgcgtggtc
     5521 ggtacgttaa accgatgccg tttgaaaggc ttttgcttca caaccgtttg gtttgatcgg
     5581 gcaggcaggg ttttccctca gccggcagag atacgcttaa tgccgtacgg acacaccgcc
     5641 gccccgtttt cagacggtat atatagtgga ttaacaaaaa tcaggacaag g
//
LOCUS       DAXHPG010000135         5668 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXHPG010000135 DAXHPG010000000
VERSION     DAXHPG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.135"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..208
                     /locus_tag="ACFM6F_001711"
     CDS             <1..208
                     /locus_tag="ACFM6F_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165091.1"
                     /translation="FSELYMFQQRTAHQAPHCVLPERDYPTIRFYRYKQTGFNRKGMG
                     IKSISDISRAMPSENQSPLSDGIV"
     gene            285..1232
                     /locus_tag="ACFM6F_001712"
     CDS             285..1232
                     /locus_tag="ACFM6F_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6165092.1"
                     /translation="MNILSKISSFIGKTFSLWVALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1385..2509)
                     /gene="rluD"
                     /locus_tag="ACFM6F_001713"
     CDS             complement(1385..2509)
                     /gene="rluD"
                     /locus_tag="ACFM6F_001713"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC6165093.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2508..3311
                     /locus_tag="ACFM6F_001714"
     CDS             2508..3311
                     /locus_tag="ACFM6F_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC6165094.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3369..5588)
                     /locus_tag="ACFM6F_001715"
     CDS             complement(3369..5588)
                     /locus_tag="ACFM6F_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC6165095.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFAGRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGV
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNASGIRYLDYLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1363 a         1797 c         1370 g         1138 t
ORIGIN      
        1 tttctccgag ctttatatgt ttcaacagag gacggcacat caagcaccgc actgcgtgtt
       61 gcccgaacga gactacccta cgattagatt ctatcgctat aaacagacgg gtttcaaccg
      121 aaaaggaatg gggataaagt ccatttccga catctctcgg gcgatgccgt ctgaaaacca
      181 atctccactt tcagacggca ttgtttaggc acggtcattt tgcaaattaa ggttaaggcg
      241 tgtatgcttg ccttccctta aaaacaaaca cgcaacgcat tattatgaat atcctcagta
      301 aaatcagcag ctttatcgga aaaacatttt ccctctgggt cgcgctcttt gccgccgccg
      361 cttttttcgc gcccgacacc ttcaaatggg cggggcctta tattccttgg ctgttgggca
      421 ttattatgtt cggtatgggt ttgacgctca aaccttccga cttcgatatt ttgttcaaac
      481 atcccaaagc cgtcatcatc ggcgtaatcg cacaattcgc cattatgccg gcaaccgcct
      541 ggctgctgtc caaactgttg aacctgcctg ccgaaatcgc ggtcggcgtg attttggtcg
      601 gctgctgccc gggcggtacg gcttccaatg tgatgaccta tctggcgcgc ggcaatgtgg
      661 ctttgtcggt tgccgttacg tctgtttcca ccctgatttc cccattgctg actcccgcca
      721 tcttcctgat gcttgccggt gaaatgctgg aaatccaagc ggccggtatg ttgatgtcca
      781 tcgtcaaaat ggttttgctc cccattgttt tgggtttgat tgtccataag gttttgggca
      841 gtaaaaccga aaagctgacc gatgcgctgc cgctggtttc cgttgccgcc atcgtgctga
      901 ttatcggcgc ggttgttggg gcaagcaaag gcaagattat ggaaagcggc ctgctgattt
      961 ttgcggttgt cgtactccac aacggcatcg gctacctgct cggcttcttt gccgccaaat
     1021 ggaccggcct gccttatgat gcacaaaaaa cgctggccat cgaagtcggt atgcaaaact
     1081 cgggtctggc cgccgcgctg gccgccgcac actttgccgc tgcgccggtc gttgccgttc
     1141 cgggcgcatt gttcagcgtg tggcacaata tctccggctc gctgctggca acttattggg
     1201 cggccaaagc cggtaaacat aaaaaaccct aaacgccttt catcataatg ccgtctgaac
     1261 ggggcaaaca aaactggcgg tttctgcccg gccccttcag acggcctttg tttgaaatcg
     1321 gcggggacga ttgcccggct gctccggttc ggctgcctgc gttcgccccg cctttcaagc
     1381 cacctcactc ccttacataa accacttcga catcgtagtc gtcttcgttc caatcgtcat
     1441 cgtcgtccgc gccgaatttg tcctgccatt cctcttcatt gctcaaagac gaatccaaac
     1501 cggcttcaag acggaggaca gacaataaat gatatatgtc gtccggaatc ggtgcctcaa
     1561 acgaaacggt ttcgccgctt tccggatggg taaaactcaa gcggtaggcg tgcaatgcct
     1621 gacgcgcacc caaactttta acggcttctt tcaccgtgtc gccgcacgga tggcgcgggt
     1681 tgccgtaaac cgggtcgccg gcaagcggat ggttggcctc acgcatatgg acgcggattt
     1741 ggtgcgtcct gcctgtttcg agcgagcatt cgatatagct gtgggcaaga tagcgttcca
     1801 acactttgac gtgggtaacg gctggtttgc cgccgaattt gacgactgcc attttcaggc
     1861 ggttgtgcgg atcgcgtccg atttgggttt cgattttacc gtcaaagggg acgatgccgt
     1921 tggcgacggc gcggtagatg cgtttgaccg tgcgttcttg aagctgcctc acgagggaat
     1981 tttgcgccga cagggttttg gcaaccacca tcagcccgct ggtttctttg tccaaacggt
     2041 gtacgatgcc cgcgcgcgga atttgggaca gctcggggca atgcgccaac aggccgttga
     2101 gcagcgtccc cgtccagttg cccgccgccg gatgcaccac cagtccggcc ggtttgttga
     2161 cgacgatgac ggtatcgtct tcgtaaacaa tatccaaagc cataggctct ggaacaaacg
     2221 ccagattttc ctcactcgga cggacggtta cacaaatttg ctcgccgcct atcattttgt
     2281 ctttgggttg cgaaggttta tcgtttacaa taaccgcgcc ttctttaatc catgatgtca
     2341 ggcggctgcg cgagtagtcg ggcagaagtt tcgccaacac cgcatccaac cgcccgccgg
     2401 caagctcgag cggaacggtc agaccgatac ggttttcagt ttcggaagct gacgcaaagt
     2461 ctgaatcgtc gctataatcg gattcattat caaaggaagt attctgcatg aaaaaaattc
     2521 ttttaacggt ttcattaggt ttggcactga gtgcctgtgc cactcaaggt acggtcgata
     2581 aagatgctca gattacccaa gattggagtg tggagaagct ctatgccgaa gcccaggacg
     2641 aattgaacag cagcaattat acgcgggctg tcaagttata cgaaatcttg gaatcgcgct
     2701 tccccaccag ccgccatgcc cggcaatccc aactggatac cgcatacgcc tattataaag
     2761 acgatgaaaa agacaaggct ctggcggcaa tcgaacgctt ccgccgcctc catccgcagc
     2821 atccgaatat ggattacgcg ctgtatctgc gcggcttggt gctgttcaac gaagaccagt
     2881 ccttcttgaa caaactggcc tcgcaagact ggtccgaccg cgacccgaaa gccaaccgcg
     2941 aagcgtatca ggcctttgcg gaactcgtcc aacgcttccc gaacagcaaa tacgccgccg
     3001 atgcaaccgc acgcatggtc aaactggtcg atgcactggg cggcaatgaa atgtcggtgg
     3061 cgcgttacta catgaaacgc ggcgcatata tcgccgccgc caaccgcgcc caaaaaatta
     3121 tcggcagcta tcaaaataca cgctatgtcg aagaatcgct cgccatattg gaacttgcct
     3181 accaaaaact cggcaaaccg cagcttgccg ccgatacgcg ccgcgtgttg gaaaccaact
     3241 tcccgaaaag cccgtttttg acgcacgcct ggcagcccga cgatatgccc tggtggcgtt
     3301 actggcatta agcccatctt cgtaatgccg tctgaagccg tttcagacgg cattttatgt
     3361 ttcaaacctc attcaaacgg ttttttctgc caatagaatt tataccctct caaacgttga
     3421 gccttcacgc cgccgcgtcc caagccgaat tgcagtgcgc ccgacaaatc ggtacgcagc
     3481 agtttgatgc cgtgtgcgcg gacacggttc tgcaccgctt cggtcggatg tttgtaggcg
     3541 ttggcataac cgcttgaagc aacggcatat tcgggcgaga cggcattgag gaacacgccc
     3601 gacgaggacg tattgctgcc gtgatgtccc aaaaccaaaa cctggctgta caggttgcct
     3661 ccatacttgc cgaccaggct ttcctcgccc ttcgtatcca aatcgcccgt taccagcagt
     3721 gccgcaccgc ccgccacaac acgcaaaaca caacttttcc cattatcatc gatgtttttg
     3781 cgttcagacg gcatcaaaaa ctcaaaatcc acgccgtccc attgccaacg ctgttccgca
     3841 caatgccgcg ccccctcata aaattccggc tgcccggcat aaatcccgcc gttcggtatc
     3901 ttgccgactg cctgaaaacc gccgtcgtgg tcgctgtcgt gatgcgacaa aaccaaataa
     3961 tccaaatacc tgattcccga cgcattcaag ctcggcacaa tccccatctg cgccgccgct
     4021 gccgttccgg tgtcaaacaa aagatgatga tttgccgtct gaaccgacac cgacaaaccc
     4081 tgccccgcat cccaaaccgt aaccgcaacc tcattttccg gcacgccggg tgaacggtaa
     4141 aacacaaacc ctgccaacag caacaccgcc cacggacgca aacccaagcc gcgcggcagc
     4201 agcaacagca aagcggcaca caccgccaac accaacagcg gcaaaggtgc ggaggcaacg
     4261 gcaaactcgg gcgacacatc ggcaagccac accaaaaacc gcaaagtata ttccgccaaa
     4321 aatgccccca actgttgcaa aggcgcaaac ggcacgaccg aacccagcaa cgccagcggc
     4381 gtcaataccc aagaaaacca cggaattgcc accgcattga ccaaagggct gaccaaaggc
     4441 agcgaagcaa acagataacc gagcaaaacc agcgacaaca ccgaagccgc ccactgcccg
     4501 cgcaaagcag tttgccgttt tccctcgtgc aaacgccccg agcacgccca tatcagggcc
     4561 gccaccaaac cgaaagacag ccaagtcccc acacccaaga ccgccgacgg gtcgaacagc
     4621 agcaccgccg ccaacgcctg ccaccacgtc gcccacgccg acaatcttcc cctgcgccaa
     4681 gcccacgcaa acgccgccaa catcaaaacg ctgcgctgcg tcggcacgga aaaaccggca
     4741 agcagcgcgt aaaacagcgc gcctgcacac cccgccgcca aaacccacgc gcgcggccgg
     4801 gcaggaaggc gcggggaaca ggcaagcagc cgcttcgcca gccacgcaaa cattaccgcc
     4861 accatcgtaa cgtgcaaacc cgaaatactg accaaatgcg tcagccccaa cggccggaac
     4921 gcctgccaca attcggggcg caatgccgac tgttcgccca cgctcaacgc gcgcatcagc
     4981 ccgatgccgt ctgaaagccc gccgtccgca tccgcctgct gccaattacg gctgatgcgg
     5041 ctgcgccaaa ccgcaatccc ccaaccgctt ccgccatgca gcaaaaccct gtccgcaccg
     5101 accgtcccca cgccgcctat tccgttggat aatgcccacg cctcacggtt caaaccgcgc
     5161 aggttcaact ccccgacgac agggtgcaca cgtgccgtta tccgccatct gctcccgacc
     5221 gcccattcgc gccgtttgta gtccgacagc agcaaatcaa acgtccgacc accgctgtcc
     5281 acagcctttg ccgcaaactg cacacgccgc ccgtccgacc tcggcatatc cgccacttcc
     5341 accgtcaaag gcacgcctga aacggcttct gcccaccatt gggaagacaa tgccgcctcc
     5401 gtcctgaata cgccgtaagc cgcgcccgcc aacacgcaca gcatcagacc ggcaaacgca
     5461 aaacgccctg caaacacagc aaaaaccgca aaagccgcca gccaaaacag ccaatgcggc
     5521 acgaccggca gcgcaaacga agccgccgcg ccgacaaccc aacacggcaa aaaccaacga
     5581 cggaacacgt ctccccaccc cagatatttc aaaaataaac ggataataaa gcaaatccga
     5641 aaaaccgtca taacaagatt tgaaaatg
//
LOCUS       DAXHPG010000136         5631 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXHPG010000136 DAXHPG010000000
VERSION     DAXHPG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.136"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            72..998
                     /gene="fabD"
                     /locus_tag="ACFM6F_001716"
     CDS             72..998
                     /gene="fabD"
                     /locus_tag="ACFM6F_001716"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC6165096.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYSEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICTESAQDEVVEAVNFN
                     SPGQVVIAGNAAAVGRAMTAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKTAACSALTDAGQVAAFIEAH"
     gene            1137..3002
                     /gene="msbA"
                     /locus_tag="ACFM6F_001717"
     CDS             1137..3002
                     /gene="msbA"
                     /locus_tag="ACFM6F_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC6165097.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIIAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            3102..4667
                     /gene="guaA"
                     /locus_tag="ACFM6F_001718"
     CDS             3102..4667
                     /gene="guaA"
                     /locus_tag="ACFM6F_001718"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC6165098.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIVGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            4729..5475
                     /gene="fabG"
                     /locus_tag="ACFM6F_001719"
     CDS             4729..5475
                     /gene="fabG"
                     /locus_tag="ACFM6F_001719"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC6165099.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMVREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         1374 a         1816 c         1366 g         1075 t
ORIGIN      
        1 ttttgtcccg cctgaatttt tcgtaaaact cgggcagaat acctgattat ccaaccaaat
       61 aaaggaatac tatgtctttt gccttctttt ttcccggaca aggttcccaa agcctcggta
      121 tgatgaacgg ctttgccgaa cacgccatcg tcaaaaacac atttgacgaa gcctccgcca
      181 tattggggca ggacttgtgg gcgatgatta acggcagcga tgccgaaatc atcggtcaaa
      241 ccgtcaacac ccagcccatt atgctcgccg ccggcgttgc cgtttaccgc gcctattcgg
      301 aagcgggcgg caaaacgcct gccgccgttg ccggacacag cctcggcgaa tacaccgcac
      361 tcgttgccgc cggcgcattg gattttgccg acgcggtcaa actcgtgcgc ctacgcgccg
      421 aactgatgca gtccgccgta ccgcaaggcg tgggcgcaat ggcggcaatt ttgggtttgg
      481 aagatgaaca ggtaaggcag atttgcaccg aatccgcaca ggatgaagtg gttgaagccg
      541 tcaacttcaa ctcgcccgga caagtcgtaa ttgcaggcaa tgccgccgcc gtcggacgcg
      601 ccatgaccgc cgccaaagaa gccggtgcca aacgcgccct gccgctgccc gtgtccgtac
      661 cttcccattg cagcctcatg aaacccgccg ccgacaaact cgccgaagcc ctcaaaaccg
      721 tcgaaatcaa gcagccgcaa atccgcgtca tccacaacgc cgacgttgcc gcctacgatg
      781 atgccgacaa aatcaaagac gcgctcgtcc gccagcttta cagccccgta cgctggacgg
      841 aaaccgtcaa cgcccttgtt tcagacggca ttgccgaatc cgccgaatgc ggaccgggca
      901 aagtgttggc gggcttggca aaacgcatca acaaaaccgc cgcgtgcagc gcgctgaccg
      961 atgccggaca ggttgccgcc tttatcgaag cgcactgact tcgttccgca aaaagcagcc
     1021 tgccctcttc aggctgcttt tcacgtccga acggcaaaca gccgcatatt tacgctataa
     1081 tccatcccga ccaaaccacc gacagcggct gccgttgcag tttccgcccc accgatatga
     1141 tagaaaaact gactttcgga ctgtttaaaa aagaagacgc gcgcagcttt atgcgcctga
     1201 tggcatacgt ccgcccctac aaaatccgca tcattgccgc cctgattgcc attttcggcg
     1261 ttgccgccac cgaaagctac cttgccgcct tcatcgcccc cctgattaac cacggctttt
     1321 ccgcacctgc cgcgccgccc gagctgtctg ccgccgccgg catcatttcc accctgcaaa
     1381 actggcgcga acagtttacc tatatggttt gggggacgga aaacaaaatc tggaccgtcc
     1441 cgctcttcct catcatcctc gtcgtcatcc gtggcatctg ccgctttacc agcacctatc
     1501 tgatgacttg ggtctccgtg atgaccatca gcaaaatccg caaagatatg tttgccaaaa
     1561 tgctgaccct ttcctcccgc taccatcagg aaacgccgtc cggcaccgta ctgatgaata
     1621 tgctcaacct gaccgaacag tcggtcagca acgccagcga catcttcacc gtcctcacgc
     1681 gcgacacgat gatcgttacc ggcctgacca tcgtcctgct ttacctcaac tggcagctca
     1741 gcctcatcgt cgtcctgatg ttccccctgc tctccctgct ctcgcgctac taccgcgacc
     1801 gtctgaaaca cgtcatttcc gactcgcaaa aaagcatagg cacgatgaac aacgtgattg
     1861 ccgaaaccca tcagggacac cgcgtcgtca agctgttcaa cgggcaggcg caggcggcaa
     1921 accggttcga cgcggtcaac cgcaccatcg tccgcctcag caaaaaaatc acgcaggcaa
     1981 cggcggcaca ttccccgttc agcgaactga tcgcctcgat cgccctcgcc gtcgtcatct
     2041 tcatcgccct gtggcaaagc caaaacggct acaccaccat cggcgaattt atggcattca
     2101 tcgtcgcgat gctgcaaatg tacgccccca tcaaaagcct tgccaacatc agcatcccta
     2161 tgcagacgat gttcctcgcc gccgacggcg tatgtgcatt tctcgacacc ccgcccgaac
     2221 aggacaaagg cacgctcgcg ccgcagcgtg tcgaagggcg tatcagcttc cgcaacgtcg
     2281 atgtcgaata ccgttcggac ggcatcaaag ccctcgacaa cttcaacctc gacatcagac
     2341 aaggcgaacg cgtcgccttg gtcggacgtt ccggcagcgg caaatccacc gtcgtcaacc
     2401 tgctgccccg ctttgtcgaa ccgtctgccg gcaacatctg catagacggt atcgacatcg
     2461 ccgacatcaa actcgactgc ctgcgcgccc aattcgccct cgtctcccaa gacgtattcc
     2521 tgtttgacga caccctgttt gaaaacgtcc gatacagccg tcccgacgca ggcgaagccg
     2581 aagtcctgtc cgccctccaa gccgccaacc tgcaaagcct gattgacagc tccccgctcg
     2641 gactgcacca gcccatcgga tcgaacggca gcaacttgtc cggcggacag cggcaacgcg
     2701 tcgccatcgc ccgcgccatt ttgaaagacg cgccgatatt attattggac gaagccacca
     2761 gcgcattaga caacgaatcc gaacgcctcg tccaacaagc gctcgaacgc ctgatggaaa
     2821 accgcaccgg catcatcgtc gcccaccgcc tgaccaccat cgaaggggcc gaccgcatca
     2881 tcgtaatgga cgacggcaaa atcatcgaac aaggcacaca cgaacaactg atgtcccaaa
     2941 acggctacta cacgatgtta cgcaatatct caaacaaaga tgccgccgtc cggacggcat
     3001 aaacaaaatg ccgtccgaaa tggtacaatc gccccgaccc tttcagacgg cactatatcc
     3061 gccgacccat ccgattatct tcaatcactg taaaacccat tatgacccaa gacaaaatcc
     3121 tcatcctcga cttcggttct caagttaccc agctgattgc ccgccgcgtg cgcgaagccc
     3181 acgtttactg cgaactgcat tccttcgata tgcctttgga cgaaatcaaa gccttcaacc
     3241 ccaaaggcat catcctctct ggcggcccca attccgttta cgaatccgac tatcaagccg
     3301 ataccggtat ttttgatttg ggcattccgg ttttgggcat ctgttacggc atgcagttta
     3361 tggcgcacca cttgggcggc gaagtgcagc ccggcaacca gcgcgaattc ggttatgcgc
     3421 aagttaaaac catagacagc gagctgacac gcggcattca agatggtgag ccaaacacac
     3481 tcgacgtatg gatgagccac ggcgacaaag tgtccaaact gcccgacggt ttcgccgtca
     3541 tcggcaacac cccgtcctgc ccgattgcca tgatggaaaa cgccgaaaaa caattctacg
     3601 gcatccagtt ccaccccgaa gttacccaca ccaaacaagg ccgcgccctg ttgaaccgct
     3661 ttgtcttgga tatttgcggc gcacaaccgg gctggacgat gccgaactac atcgaagaag
     3721 ccgttgccaa aatccgcgaa caggtcggca gcgacgaagt gattttaggt ttgtcaggcg
     3781 gcgtagactc ttccgtagcc gccgcgctga ttcaccgcgc catcggcgac caactgacct
     3841 gcgtgtttgt cgatcacggt ttgttgcgcc tgaacgaagg caaaatggtg atggatatgt
     3901 tcgcccgcaa cttgggtgtg aaagtgattc acgtcgatgc cgaagggcag tttatggcga
     3961 aactcgccgg cgtaaccgac cccgagaaaa aacgcaaaat cgtcggcgcg gaatttatcg
     4021 aagtatttga tgccgaagag aaaaaactca ccaacgccaa atggctggcg caaggtacga
     4081 tttaccctga cgtaatcgaa tccgcaggcg cgaaaaccaa aaaagcccac gccatcaaat
     4141 cgcaccacaa tgtcggcggc ctgcctgaaa acatgaagct caagctgctt gagccgttgc
     4201 gcgacttatt caaagacgaa gtgcgcgaac tcggcgtcgc tttgggcttg ccgcgcgaaa
     4261 tggtgtaccg ccatccgttc cccggtcccg gtttgggcgt gcgcatcttg ggcgaagtga
     4321 aaaaagaata cgccgactta ttgcgtcaag cagacgatat tttcatccaa gaattacgca
     4381 atactaccga cgaaaacggc acatcttggt acgacctgac cagccaagcc ttcgccgtct
     4441 tcctgcccgt aaaatccgtc ggcgtaatgg gcgacggacg cacttacgac tacgtcgtcg
     4501 cactgcgcgc agtcatcacc agcgacttca tgaccgcaca ctgggcagaa ctgccttact
     4561 cactgctcgg acgcgtgtcc aaccgcatca tcaacgaagt caaaggcatc aaccgcgtgg
     4621 tgtacgatgt cagcggcaaa ccgcctgcca ccatcgagtg ggaataacca gcaaacaggg
     4681 ctgcaccgtc cggcgcagtc tttcgattat cggaaaaaag gaaaaaatat gagcacacaa
     4741 gatttaagcg gcaaaatcgc tttggtaacc ggcgcatcgc gcggtatcgg tgcagcaatt
     4801 gccgacacac tggcggcagc cggtgccaaa gtcatcggta cggcgaccag tgagagcggt
     4861 gcggcggcga ttagcgagcg gttggcgcaa tggggcggcg aaggccgcgt attaaattcc
     4921 gccgaacccg aaaccgtcga aaacctgatt gccgacatcg aaaaaacgtt cggcaaactc
     4981 gatattctgg tcaacaacgc cggcatcacc cgcgacaacc tcctgatgcg catgaaagaa
     5041 gaagagtggg acgacatcat gcaggtcaac ctcaaatccg tgttccgcgc ttctaaagcc
     5101 gttttgcgcg gtatgatgaa acaacgttcc ggccgcatca tcaacatcac atccgtcgtc
     5161 ggcgtgatgg gcaatgccgg tcaaaccaac tatgccgcgg caaaagcagg cttaatcggt
     5221 ttctccaaat ccatggtgcg cgaagtcggc agccggggca ttaccgtcaa ctgcgtcgcc
     5281 cctggcttta tcgataccga catgacacgc gccctgccgg aagaaacccg ccaaaccttt
     5341 accgcccaaa ccgccttggg cagattcggc gacgcgcaag acatcgccga tgcggttctg
     5401 ttcctcgctt ccgaccaagc aaaatacatc accggccaaa cgctgcacgt caacggcggt
     5461 atgctgatgc cctgacgggc agccgtaccg acaatgccgt ctgaagccct ttcagacggc
     5521 atttgcattc tcaggcaaaa tgaacacacg ccacaccccg ccctgcccct gcggatcagg
     5581 cacaagctga gacctttgca aaactccttt ccctcccgac agccgaaacc c
//
LOCUS       DAXHPG010000137         5570 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXHPG010000137 DAXHPG010000000
VERSION     DAXHPG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.137"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..1647
                     /gene="hrpB"
                     /locus_tag="ACFM6F_001720"
     CDS             <1..1647
                     /gene="hrpB"
                     /locus_tag="ACFM6F_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HFC6165100.1"
                     /translation="SMQRQQHIDAELLTDANVRFEQPLEKNNYVLSEDETPCTRVNYI
                     SLDDKTARKFSFLPSVLMKETAFKTGMCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQ
                     PQNMDSGILKLRVSAGEIGDIRYEEKRDGKSAEGSISAFNNKFPLYRNKILNLRDVEQ
                     GLENLRRLSSVKTDIQIIPSEEEGKSDLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGN
                     VALSFDNPLGLSDLFYVSYGRGLAHKTDLTDATGTETASGSRSYSVHYSVPVKKWLFS
                     FNHNGHRYHEATEGYSVNYDYNGKQYQSSLAAERMLWRNRLHKTSVGMKLWTRQTYKY
                     IDDAEIEVQRRRSAGWEAELRHRAYLNRWQLDGKLSYKRGTGMRQSMPAPEENGGGTI
                     PGTSRMKIITAGLDAAAPFMLGKQQFFYATAIQAQWNKTPLVAQDKLSIGSRYTVRGF
                     DGEQSLFGERGFYWQNTLTWYFHPNHQFYLGADYGRVSGESAQYVSGKQLMGAVVGFR
                     GGHKVGGMFAYDLFAGKPLHKPKGFQTTNTVYGFNLNYSF"
     gene            1756..>5570
                     /gene="hrpA"
                     /locus_tag="ACFM6F_001721"
     CDS             1756..>5570
                     /gene="hrpA"
                     /locus_tag="ACFM6F_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HFC6165101.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFVVKGSAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGEADYTGVLARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNDGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNII
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAA"
BASE COUNT         1660 a         1382 c         1299 g         1229 t
ORIGIN      
        1 tccatgcagc gtcagcagca catagatgct gaattgttaa ctgatgcaaa tgtccgtttc
       61 gagcaaccat tggagaagaa caattatgtc ctgagtgaag atgaaacacc gtgtactcgg
      121 gtaaattaca ttagtttaga tgataagacg gcgcgcaaat tttcttttct tccttctgtg
      181 ctcatgaaag aaacagcttt taaaactggg atgtgtttag gttccaataa tttgagcagg
      241 ctacaaaaag ccgcgcaaca gatactgatt gtgcgtggct acctcacttc ccaagctatt
      301 atccaaccac agaatatgga ttcgggaatt ctgaaattac gggtatcagc aggcgaaata
      361 ggggatatcc gctatgaaga aaaacgggat gggaagtctg ccgaaggcag tattagtgca
      421 ttcaataaca aatttccctt atataggaac aaaattctca atcttcgcga tgtagagcag
      481 ggcttggaaa acctgcgtcg tttgtcgagt gttaaaacag atattcagat tataccgtcc
      541 gaagaagaag gcaaaagcga tttacagatc aaatggcagc agaataaacc catacggttc
      601 agtatcggta tagatgatgc gggcggcaaa acgaccggca aatatcaagg aaatgtcgct
      661 ttatcgttcg ataacccttt gggcttaagc gatttgtttt atgtttcata tggacgcggt
      721 ttggcgcaca aaacggactt gactgatgcc accggtacgg aaactgcaag cggatccaga
      781 agttacagcg tgcattattc ggtgcccgta aaaaaatggc tgttttcttt taatcacaat
      841 ggacatcgtt accacgaagc aaccgaaggc tattccgtca attacgatta caacggcaaa
      901 caatatcaga gcagcttggc cgccgagcgc atgctttggc gtaacagact tcataaaact
      961 tcagtcggaa tgaaattatg gacacgccaa acctataaat acatcgacga tgccgaaatc
     1021 gaagtgcaac gccgccgctc tgcaggctgg gaagccgaat tgcgccaccg tgcttacctc
     1081 aaccgttggc agcttgacgg caagttgtct tacaaacgcg ggaccggtat gcgccaaagt
     1141 atgcccgcac ctgaagaaaa cggcggcggt actattccag gcacatcccg tatgaaaatc
     1201 ataaccgccg gattggatgc agcggccccg tttatgttgg gcaaacagca gtttttctac
     1261 gcaaccgcca ttcaagctca atggaacaaa acgccgttgg ttgcccaaga taaattgtca
     1321 atcggcagcc gctacaccgt tcgcggattt gatggggagc agagtctttt cggagagcga
     1381 ggtttctact ggcagaatac tttaacttgg tattttcatc cgaaccatca gttctatctc
     1441 ggtgcggact atggccgcgt atctggcgaa agtgcacaat atgtatcggg caagcagctg
     1501 atgggtgcag tggtcggctt cagaggaggg cataaagtag gcggtatgtt tgcttatgat
     1561 ctgtttgccg gcaagccgct tcataaaccc aaaggctttc agacgaccaa caccgtttac
     1621 ggcttcaact tgaattacag tttctaacct ctgaattttt tactgatatt tagacggtct
     1681 ttccttatcc tcagaccgtc aaactttacc tacgtacttg gcgcgcagta cgttcatctt
     1741 caaaatggaa tagacatgaa taaaggttta catcgcatta tctttagtaa aaagcacagc
     1801 accatggttg cagtagccga aactgccaac agccagggca aaggtaaaca ggcaggcagt
     1861 tcggtttctg tttcactgaa aacttcaggc gacctttgcg gcaaactcaa aaccaccctt
     1921 aaaactttgg tctgctcttt ggtttccctg agtatggtat tgcctgccca tgcccaaatt
     1981 accaccgaca aatcagcacc taaaaaccag caggtcgtta tccttaaaac caacactggt
     2041 gcccccttgg tgaatatcca aactccgaat ggacgcggat tgagccacaa ccgctatacg
     2101 cagtttgatg ttgacaacaa aggggcagtg ttaaacaacg accgtaacaa taatccgttt
     2161 gtggtcaaag gcagtgcgca attgattttg aacgaggtac gcggtacggc tagcaaactc
     2221 aacggcatcg ttaccgtagg cggtcaaaag gccgacgtga ttattgccaa ccccaacggc
     2281 attaccgtta atggcggcgg ctttaaaaat gtcggtcggg gcatcttaac taccggtgcg
     2341 ccccaaatcg gcaaagacgg tgcactgaca ggatttgatg tgcgtcaagg cacattgacc
     2401 gtaggagcag caggttggaa tgataaaggc gaagccgact acaccggggt acttgctcgt
     2461 gcagttgctt tgcaggggaa attacagggt aaaaacctgg cggtttctac cggtcctcag
     2521 aaagtagatt acgccagcgg cgaaatcagt gcaggtacgg cagcgggtac gaaaccgact
     2581 attgcccttg atactgccgc actgggcggt atgtacgccg acagcatcac actgattgcc
     2641 aatgaaaaag gcgtaggcgt caaaaatgcc ggcacactcg aagcggccaa gcaattgatt
     2701 gtgacttcgt caggccgcat tgaaaacagc ggccgcatcg ccaccactgc cgacggcacc
     2761 gaagcttcac cgacttatct ctccatcgaa accaccgaaa aaggagcggc aggcacattt
     2821 atctccaatg atggtcggat cgagagcaaa ggcttattgg ttattgatac cggggaggac
     2881 atcggtttgc gtaacggagc ggtcgttcaa aacaacggca accgccctgc tgctacggta
     2941 ttaaatgccg gccgtaattt ggttatcgaa agcaaagcaa atgtgaacaa tgctaaaggt
     3001 tcgaccaatc tgtcgaccgg cggccgtacc gtcatcaagg aggccagtat tcagactggc
     3061 actaccgtat acagttccag caaaggcaac gccgaattag gcaataacac acgcattacc
     3121 ggggcagatg ttaccgtatt atccaacggc accatcagca gttccgccgt aatagatgcc
     3181 aaagacaccg cacacatcga agcaggcaaa ccgctttctt tggaagcttc aacagttacc
     3241 tccgatatcc gcttaaacgg aggcagtatc aagggcggca agcagcttgc tttactggca
     3301 gacgataaca ttactgccaa aactaccaat ctgaatactc ccggcaatct gtatgttcat
     3361 acaggtaaag atctgaattt gaatgttgat aaagatttgt ctgccgccag catccatttg
     3421 aaatcggata acgctgccca tattaccggc accagtaaaa ccctcactgc ctcaaaagac
     3481 atgggtgtgg aggcaggctc gctgaatgtt accaatacca atctgcgtac caactcgggt
     3541 aatctgcaca ttcaggcagc caaaggcaat attcagcttc gcaataccaa gctgaacgca
     3601 gccaaggctc tcgaaaccac cgcattgcag ggcaatatcg tttcagacgg ccttcatgct
     3661 gtttctgcag acggtcatgt atccttattg gccaacggta atgccgactt taccggtcac
     3721 aataccctga cagccaaggc cgatgtcaat gcaggatcgg ttggtaaagg ccgtctgaaa
     3781 gcagacaata ccaatatcat ttcatcttca ggagatatta cgttggttgc cggcaacggt
     3841 attcagcttg gtgacggaaa acaacgcaat tcaatcaacg gaaaacacat cagcatcaaa
     3901 aacaacggtg gtaatgccga cttaaaaaac cttaacgtcc atgccaaaag cggggcattg
     3961 aacattcatt ccgaccgggc attgagcata gaaaatacca agctggagtc tacccataat
     4021 acgcatctta atgcacaaca cgagcgggta acgctcaacc aagtagatgc ctacgcacac
     4081 cgtcatctaa gcattaccgg cagccagatt tggcaaaacg acaaactgcc ttctgccaac
     4141 aagctggtgg ctaacggtgt attggcactc aatgcgcgct attcccaaat tgccgacaac
     4201 accacgctga gagcgggtgc aatcaacctt actgccggta ccgccctagt caagcgcggc
     4261 aacatcaatt ggagtaccgt ttcgaccaaa actttggaag ataatgccga attaaaacca
     4321 ttggccggac ggctgaatat tgaagcaggt agcggcacat taaccatcga acctgccaac
     4381 cgcatcagtg cgcataccga cctgagcatc aaaacaggcg gaaaattgct gttgtctgca
     4441 aaaggaggaa atgcaggtgc gcctagtgct caagtttcct cattggaagc aaaaggcaat
     4501 atccgtctgg ttacaggaga aacagattta agaggttcta aaattacagc cggtaaaaac
     4561 ttggttgtcg ccaccaccaa aggcaagttg aatatcgaag ccgtaaacaa ctcattcagc
     4621 aattattttc ctacacaaaa agcggctgaa ctcaaccaaa aatccaaaga attggaacag
     4681 cagattgcgc agttgaaaaa aagctcgcct aaaagcaagc tgattccaac cctgcaagaa
     4741 gaacgcgacc gtctcgcttt ctatattcaa gccatcaaca aggaagttaa aggtaaaaaa
     4801 cccaaaggca aagaatatct gcaagccaag ctttctgcac aaaatattga cttgatttcc
     4861 gcacaaggca tcgaaatcag cggttccgat attaccgctt ccaaaaaact gaaccttcac
     4921 gccgcaggcg tattgccaaa ggcagcagat tcagaggcgg ctgctattct gattgacggc
     4981 ataaccgacc aatatgaaat tggcaagccc acctacaaga gtcactacga caaagctgct
     5041 ctgaacaagc cttcacgttt gaccggacgt acgggggtaa gtattcatgc agctgcggca
     5101 ctcgatgatg cacgtattat tatcggtgca tccgaaatca aagctccctc aggcagcata
     5161 gacatcaaag cccatagtga tattgtactg gaggctggac aaaacgatgc ctataccttc
     5221 ttaaaaacca aaggtaaaag cggcaaaatc atcagaaaaa ccaagtttac cagcacccgc
     5281 gaccacctga ttatgccagc ccccgtcgag ctgaccgcca acggtatcac gcttcaggca
     5341 ggcggcaaca tcgaagctaa taccacccgc ttcaatgccc ctgcaggtaa agttaccctg
     5401 gttgcgggtg aagagctgca actgctggca gaagaaggca tccacaagca cgagttggat
     5461 gtccaaaaaa gccgccgctt tatcggcatc aaggtaggta agagcaatta cagtaaaaac
     5521 gaactgaacg aaaccaaatt gcctgtccgc gtcgtcgccc aaactgcagc
//
LOCUS       DAXHPG010000138         5486 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXHPG010000138 DAXHPG010000000
VERSION     DAXHPG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.138"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            116..637
                     /locus_tag="ACFM6F_001722"
     CDS             116..637
                     /locus_tag="ACFM6F_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
                     /protein_id="HFC6165102.1"
                     /translation="MSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAKI
                     QPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDAVNTRGYSSKPIDTLMALA
                     KDGTIAGAKLVDHHESIMLIGIPHLPAPGRAIRSNWLPAYIKPNFISTNRLRLKGLPT
                     VLQPSKGTGAGVP"
     gene            526..1725
                     /locus_tag="ACFM6F_001723"
     CDS             526..1725
                     /locus_tag="ACFM6F_001723"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HFC6165103.1"
                     /translation="MASGVYQTKLYIDKPITIEGPADRSATIEGDRSGRTIAVHAPDV
                     TLRNLTVTRSGMSLPAMDAGIYLEETAPHALIEHNNILDNSVGVYLHGSSEAMVRENK
                     IVGDATLRVNERGNGVTVWNAPGAQVVGNDISKGRDGIFSNTSTYNTYKNNRFSDLRF
                     AVHYMYTNDSEISGNISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIH
                     DNIINKAGKCVFAYNANYDKLFANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKY
                     VSTRFLDWSEGGHGNYWSDNSAFDLNGDGFGDSAYRPNGIIDQIIWRAPVSRLLMNSP
                     AISIVKWAQAQFPAVLPGGVVDSKPLMKPYAPKIQTRYQAMKDELLKEAETRQSEWGR
                     AENGSLN"
     gene            1783..2397
                     /locus_tag="ACFM6F_001724"
                     /pseudo
     CDS             1783..2397
                     /locus_tag="ACFM6F_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2414..2545
                     /locus_tag="ACFM6F_001725"
                     /pseudo
     CDS             2414..2545
                     /locus_tag="ACFM6F_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2542..3036
                     /locus_tag="ACFM6F_001726"
     CDS             2542..3036
                     /locus_tag="ACFM6F_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC6165104.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGSPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(3124..4785)
                     /locus_tag="ACFM6F_001727"
     CDS             complement(3124..4785)
                     /locus_tag="ACFM6F_001727"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC6165105.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQVIERFQLDQNSQPQTYGLGIKE
                     IWEVPSEKHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVENFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(4915..>5486)
                     /locus_tag="ACFM6F_001728"
     CDS             complement(4915..>5486)
                     /locus_tag="ACFM6F_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6165106.1"
                     /translation="NPVAYGKIKVQSWKERRDFNIVKQDLDFSCGAASVVTLLNNFYG
                     QKLTEEDVLKKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKY
                     RKDDHFSVLRGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRD
                     AGGDKAFFTRSPKRQTEFAVGQVKWWRAY"
BASE COUNT         1310 a         1478 c         1398 g         1300 t
ORIGIN      
        1 tatcccgtat gcggattggt tttaagattt gtaaatttaa tttgcatcaa aaaatcgccg
       61 atagatgatt catataatat caatattaaa gagtatcggt atatcgggga tagtcatgtc
      121 ctgtttttca atcaaacgta tgtccgcgtt tcgggcgcgg ataacggcgt tttttgccgc
      181 ctttgtcttt ttgacggcgg cactgcccgc ttatgcggag cgtctgcctg attttctggc
      241 gaaaatacag ccttcggaaa tttttccggg tgcggaccgt tacggcaagc cggaaggtaa
      301 gcctatggtt gcccgcgttt acaaaggcga tgagcagttg ggcttggtct atatcacgac
      361 cgatgcggtc aatacgcgcg gttattcgag caaaccgatt gatacgctga tggcgttggc
      421 taaagacggt acgatagccg gagcgaaatt ggttgatcac catgagtcga ttatgctgat
      481 cggtatcccg catttgcccg cgcccgggcg ggcgatacgc tcaaactggc ttccggcgta
      541 tatcaaacca aactttatat cgacaaaccg attacgattg aagggcctgc cgaccgttct
      601 gcaaccatcg aaggggacag gagcgggcgt accatagccg tacacgcgcc ggacgtaacg
      661 ctccgcaacc tgaccgttac ccgttccggc atgagcctgc cagcaatgga tgccggtatt
      721 tatctcgaag aaaccgctcc gcacgccctg attgaacaca ataatatttt ggataattcg
      781 gtcggcgtct atctgcatgg ttcttccgaa gcgatggtgc gcgagaacaa aatcgtcggc
      841 gacgcgactt tgcgcgtgaa cgagcgcggc aacggcgtta ccgtttggaa cgcgcccggt
      901 gcgcaagtcg tcggcaacga catttccaaa gggcgggacg gcattttttc caacaccagc
      961 acatataaca cctacaaaaa caaccgcttc agcgatttgc gtttcgccgt ccactatatg
     1021 tacaccaacg acagcgaaat cagcggcaat atttccgtgg gcaacaatat gggctatgtg
     1081 ctgatgtttt ccgagcggct caaagtattc gacaatatcg ccgtcggcag ccgcgatcag
     1141 ggcattatgc tcaactatgt caactattcc gatattcacg acaacattat caacaaagcg
     1201 ggcaagtgcg tatttgccta taatgccaac tacgataaac ttttcgccaa tcattttgaa
     1261 aactgccaaa tcggcataca ctttaccgcc gccatcgaag gcacgtccct gcacgacaat
     1321 tccttcatca acaacgaaag ccaggtcaaa tacgtcagca cgcgctttct cgactggagc
     1381 gagggcggac acggcaacta ttggagcgac aacagcgcgt tcgatttgaa cggcgacggc
     1441 ttcggcgaca gcgcgtaccg gcccaacggc atcatcgacc aaatcatctg gcgcgcgccc
     1501 gtatcgcgtc tcttgatgaa cagtcccgca atcagcatcg tcaaatgggc gcaggcgcag
     1561 tttcccgccg ttctgcctgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc
     1621 cccaaaattc aaacccgtta tcaggcgatg aaggacgagt tgctcaaaga agccgaaacg
     1681 cggcagtcgg aatggggcag ggcggaaaac gggtctttga actagtctgt ttcagacggc
     1741 atccggattc aaatgccgtc tgaaaacaca aaaggaacaa ccatgaccac acatcatgtc
     1801 gaattgagga aggtaaccaa acggttcggg gcgcaaaaag ccgttaacca agtcgatttg
     1861 gttttgaagg caggagaaag cgtcgggctt gccggacaca acggcgcggg caagtccacc
     1921 attatgaagc tgatactcgg gctgattacc ccgaccgaag gcgaagtgat gcttttgggc
     1981 gaacgtaccg gcagcaaagc gggggcgcgg cttcgcagcc aaatcggcta cctgcccgaa
     2041 actgttgcgt tgcacccttc gctgaccggc atcgaaacgc tggattttta tgccaaactt
     2101 aaaaaacagc cgctcacgca gaatcggggg ctgctcgagc gcgtcggcat ttcacaggcg
     2161 gcacaccgcc gcgtcggcac ttattctaaa gggatgcgcc aacgccttgc cttggcacaa
     2221 gccctgctgg gcgagcccaa agtcctgctg tttgacgaac cgacaaccgg cctcgaccct
     2281 gcatcaaggc aaatgtttta cgaagtcgtg cgcgaactca acgggcgcgg cgcgaccgta
     2341 ttgctcagta cccacgccct tgccgagttg gacgggcacg ccgaccgcat tatcgtggat
     2401 taaatttaat ccactatatg cgggtatggc gggtttgagc ggacaaatca gcctgaccgt
     2461 ccccgttttg ctgaccgctc aggttttatg ggttatcatt ccgcttgttt tggcagccgg
     2521 aatttttaga aagcgacaaa tatgaaaaaa accctgttgg caattgttgc cgtttccgcc
     2581 ttaagtgcct gccggcaggc ggaagaggga tcgccgcctt taccccggca gattagcgac
     2641 cgttcggtcg gacactattg cagtatgaac ctgaccgaac acaacggccc caaagcccag
     2701 attttcttga acggcaaacc cgatcagccc gtttggttct ccaccatcaa gcagatgttc
     2761 ggctatacca agctgcccga agagcctaaa ggcatccgcg tgatttacgt taccgatatg
     2821 ggcaatgtta ccgattggac gaatcccaat gccgacacgg agtggatgga tgcgaaaaaa
     2881 gccttttacg tcatcgacag cggctttatc ggcggtatgg gtgcggaaga cgcgctgccg
     2941 ttcggcaaca aagagcaggc tgagaaattt gcaaaggata aaggcggtaa ggttgtcggt
     3001 ttcgacgata tgcctgatac ctatattttc aaataatatt gaaaaaaccg gccgaaactt
     3061 taaaaacgag tttcggtcgg tttttctttt cttgttcggt atagtgtcgg caggaaagaa
     3121 ccttcacatc ccgccgtaat tcggcccgct cgcgccttcg gggcaaatcc aagtgatgtt
     3181 ttgcgtcggg tctttgatat cgcaggtttt gcagtgcacg cagtttgccg cgttgatttg
     3241 caggcgcgga ttgccgtttt cttcaacaat ttcgtacacg ccggccggac aatagcgcgt
     3301 ttcgggcgag gcgtattctt tgtagttcac gtctatcatc gtttgcggat tgttcagcac
     3361 caaatggtcg ggctggtttt cttcgtgcgc gagattggca aggaagacgc tgctcaagcg
     3421 gtcgaaggtc aacacgccgt cgggtttcgg ataatcaatc ggcttgcacg cggcggcttt
     3481 tttgagctgc tcgttgtctt tgccgtgatg tttcaaggtc cacggggctt tgcctctgaa
     3541 aatcatctga tcgatgccgg tgtagattga gccgaggtaa acgccccatt tgaatgacgg
     3601 acggacattg cgcgcggcgt aaagctcttg atacagccag ctttgttcaa aacgttgctg
     3661 ataatccgcc gcctctttgc cgctgtcgaa gttttccact tcttcaaggt tttccaacaa
     3721 ggggaacacg gcttctgcgg cgagcatggc ggatttcatc gcggtatgaa tgcctttgat
     3781 gcgcggcata ttgaggaagc ctgccgcgtc gccgattaaa acgccgcctt tgaacgagag
     3841 cttcggcaaa ctttgcaaac cgccttcaat cagcgaacgc gcgccgtaag cgatgcggcg
     3901 gccgccttca aaggttttgc ggatttcggg atgggttttg aaacgttgga actcttcaaa
     3961 cggcgacaga taagggtttt gatagtccaa accgaccacg aaaccgacgg cgactttgtt
     4021 gtcatcgaaa tggtaaacaa acgcgccgcc gtaggttttg ctgtccagcg gccagcccgc
     4081 gctgtgcacc accaagccgg gctgatgttt ttcagacggc acttcccaaa tttctttgat
     4141 gcccaagccg taagtttgcg gctggctgtt ttggtcgagt tggaaacgtt cgatgacttg
     4201 tttggaaagc gaaccgcgac aaccttcggc aaacagagtt tgctgcgccc aaagctccat
     4261 gccgggctgg aaactgtcgg tcggctcgcc gtctttgcca atgcccatat tgccggttgc
     4321 aatgcctttg accgaaccgt cttcgtgata cagcacttcg gcggcggcaa agcccggata
     4381 aatttccacg cccaaatttt ccgcctgctc cgccaaccag cgcacgactt cgcccaagct
     4441 gacgatgtag ttgccgtgat tgtcgaaatt tggggtaata ggcagattga acgctttttt
     4501 ctcagtcagg aacaacactt tgtcctgcgt taccgtacgc gtcagcggcg cgcctttttc
     4561 tttccagtcg ggaatcaact cattcagcgc aatcggatcg ataaccgcgc ccgccagcga
     4621 atgtgcaccc gcctccgaac ctttctccac cacgcaaacg ctgatttcgc gcccattttg
     4681 ttcggcaagc tgcttgagtt tgatggcggc ggacaaaccc gatgggcctg cgccgacaat
     4741 cacgacatcg tattgcatac tgtcgcgggt gatggattct gtcatggcgg ttcctgtgta
     4801 tttattattg aattgcaaat ccgtaattat acaacgagaa catatagtta ccaaatacaa
     4861 caaaggtcgt ctgaaaacca tattttcggt tttcagacga cctttgtcga aatttcaata
     4921 agcacgccac cattttacct gtccgaccgc aaactccgtc tgacgtttcg gactgcgtgt
     4981 gaaaaacgcc ttatccccgc cggcatccct ccctttcggc acaaccgcca aaatcttacc
     5041 tgccaaattt ccctcacggg tttgccaagc atccaaaaac tgcgccctgc tcattgaaac
     5101 atgacccagc gacgggtcgg caagcaaaac cgtattgccg tctataccgc gcaataccga
     5161 gaaatgatca tccttgcggt atttcagata cacgatgacg gggatttgca actgtgcaag
     5221 ctgctcgaaa gacagggcat agcctttcgc ttcaaaaccc aaatcaggca taatgcgccg
     5281 catatcctca aacgacgcgg gcatctgctc cttatccagt ttttttaaca cgtcctcttc
     5341 cgtcagcttt tgcccgtaaa aattgttcaa aagcgtcacc accgaagccg ccccgcagga
     5401 aaaatccaaa tcctgcttta caatattgaa atcgcgcctt tctttccaac tctgcacttt
     5461 gatttttcca taagcaacag gattat
//
LOCUS       DAXHPG010000139         5457 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXHPG010000139 DAXHPG010000000
VERSION     DAXHPG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.139"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..891)
                     /gene="mafA"
                     /locus_tag="ACFM6F_001729"
     CDS             complement(<1..891)
                     /gene="mafA"
                     /locus_tag="ACFM6F_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6165107.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVE"
     gene            complement(1075..1794)
                     /gene="pyrH"
                     /locus_tag="ACFM6F_001730"
     CDS             complement(1075..1794)
                     /gene="pyrH"
                     /locus_tag="ACFM6F_001730"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC6165108.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(2013..2867)
                     /gene="tsf"
                     /locus_tag="ACFM6F_001731"
     CDS             complement(2013..2867)
                     /gene="tsf"
                     /locus_tag="ACFM6F_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC6165109.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(2996..3724)
                     /gene="rpsB"
                     /locus_tag="ACFM6F_001732"
     CDS             complement(2996..3724)
                     /gene="rpsB"
                     /locus_tag="ACFM6F_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC6165110.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAIRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(3901..4134)
                     /locus_tag="ACFM6F_001733"
     CDS             complement(3901..4134)
                     /locus_tag="ACFM6F_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165111.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            4212..4793
                     /locus_tag="ACFM6F_001734"
     CDS             4212..4793
                     /locus_tag="ACFM6F_001734"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC6165112.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            4854..5444
                     /locus_tag="ACFM6F_001735"
     CDS             4854..5444
                     /locus_tag="ACFM6F_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC6165113.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         1216 a         1244 c         1524 g         1473 t
ORIGIN      
        1 ctctacggat ggggcggagt tacccgtatg attgccgtat ggtcggatat cggagaaatc
       61 gaccattaat ccttccgtcg gtttaattcc tttgcttact ttatacggcc ccatccacaa
      121 tgcgtaattt tctttatagg cagcttcaaa cgcattggtt ttgggtttga tgagcaattt
      181 tttattggtt ctgtctactg cgaaatattc cagttttgtt tgggctttca gtgtttcggc
      241 attgtatagg tgcatttcgg ttctgttgcg tatcgttccg aatacgtcga tgttaataaa
      301 cacatctgta tcggcattgg caggagaaac aacgtctatg ccgcgcagga aaaatacggt
      361 ctgtaccaag tgggaaagaa aggcagtgtc gcgcgggtta gtcgtcaagg tttcatttcg
      421 ataatccccc atcccgccaa tatttaagcc cagactgctc ctacttccgc taccgtctga
      481 ttgggtgcgc gagcgtgcag gggcattaag tgtagataaa gaagtggtta aacctgtcaa
      541 accgcctgat gttgtttcag cggtggtttc gtaacgtgga taggtgtaat cggtacggac
      601 ggcagggctg tttatgtatt cgccacgaat cagtgcatca atggagtagc gaccccctgt
      661 caaactgcct gaaccttggt cgcccatagt tgcaatgtac aatgcaactt ttcgtccgtg
      721 taatgcctgt aaatccatgt ctttaacggc agctctggca gaagcggcca caagttcttg
      781 ttcgaccgca aagcgtttac ctccgccatg cgatggaata cctgtcagtg tcccgcaggc
      841 ggataaaata aaaactgaaa aaagaatagg tatcagcagc cgtgcttgca tagattttct
      901 cctttgatga aaaacaaatt gtatcaaaat tgtaaatata gtggattaaa tttaaaccag
      961 tacggcaagg cgaggcaacg ccgtactggt ttaaatttaa tccactataa tctccatata
     1021 ataaatggct gtctgaagcc catatgcgac tatatctgcc gacactatgg tcaatcagca
     1081 gtgaaccagc gtaccttcgt tttcaccggt tactacacgt ttgagcgagc cttctttagc
     1141 aatgccgaat actacaatat tgagcttgcg ttcgcggcag agggcgaaag cggtggcatc
     1201 catcactttg aggtttttca acaaggcttc gtcaaaagta atggtttcgt agcgcgtggc
     1261 ggatgagtct tttttggggt ctgcggtgta cacgccgtca acgttggtgg ctttgagcat
     1321 cacgtcgcag ttcatttccg caccgcgcaa tgcggcggca gtgtcggtcg tgaagaacgg
     1381 gttaccggta ccggcggcaa aaatcacgac tttgccttct tccaaatatt ggatggcttt
     1441 aggacgggcg taagtttcag cgatttgctg catagacagt gcggattgta cgcgcgcttt
     1501 gatgcctaaa gtttcaaatg cgtctttgag tgccaacgcg ttcatcacgg tcgccatcat
     1561 gcccatgtag tcggcggtgg cgcgatccat gctgcctgct tgggcggata cgccccggaa
     1621 aatattgccg ccgccgacaa caataccgac ttgcacgccc attttaacga cttcggcaat
     1681 ttcgccgaca gtttgaacga tggtatcgtg attgatgccg aacggatcgg aacccatcag
     1741 ggattcgccg gagagtttca gtaatacgcg tttgtatttg atttgctgtg tcatgggata
     1801 ccttgctttc ttgaatgtcg tcggtcggag tttgggccgt ctgaatttca aacggtcgga
     1861 atattaatga tagggtgtgt cggtttatta ttgttttgtt ttcagacggc ctttatattc
     1921 ggtcgtctta aaataggtac cgtaaacggt tttctcaaaa aaaagcaccc tgattcgttt
     1981 ggaatccagg tgctttcttt ttcataagtg ccttacactt tggcggctgc ggctacttcg
     2041 gctgcgtagt cgacaacggc tttttcgatg ccatcgccta ctttgtagcg tacgaagctg
     2101 accacttcag tgccgttttc tttggcgaat tgggcaacag tttgatcagg gttcatcacg
     2161 aatgcttggc cgttcagagt gatttcagcc aagaatttgc ggatacggcc ttcaaccatt
     2221 ttagcggcga tgtcggcagg tttgccggaa gcgatggctt gttcggtgta gatgtggcgt
     2281 tctttttcaa cggtttcagc atctacttcg gcttcgctta cgcattgtgg tttggcggca
     2341 acaatgtgca taccgatttt gcgtgctacg tcttcagagc ctttgtactc aaccaatacg
     2401 ccttcggtcg ccaatgcgcc gtggatgtag gcaaccagtt ggttggcagt gtcgatcact
     2461 tggaagcgac ggacagacat gttctcgccc aatttggcga tgatggcttt gcgttctgct
     2521 tcaaccagtt cgctcagttc ttcaacagaa gccggttttt tctcggcagc agttttcgca
     2581 acgaagttgg caaattctac gaagcccgcg tctttagcaa cgaagtcggt ttcgcagttt
     2641 acttcaacca atgcgccgac attgccgttg attgcataag ccaatacgcc ttcggcagcg
     2701 gtacggccgg ccagtttacc ggctttcgca ccggatttga tacgcaggat ttcttcggct
     2761 ttgtcgaagt tgccttcggc ttcaaccaag gcttttttgc attccatcat gcccaggccg
     2821 gtagcggcgc gcagatcggc aaccattttt gcagtaattt ctgccatttt gaatctccta
     2881 gatttttggg aaatacggaa aggattttcc gcattcggga atcgtgtgcc gcgaacggac
     2941 gttcagacgg catatttgag aaaaggggca taacgcccct cttcggtttg ccagattact
     3001 cggcagcggc ttcttgggca gcggctacgg tttcttgcag cgcttggttt ttgccttcca
     3061 aaactgcgtc agcgatgccg cggcagtaca ggcggatggc tttggcggag tcgtcgttgc
     3121 cggggatgac gtatttcacg ccgtcagggc tgttgttggt atcgactacg gcgataacgg
     3181 ggatgcccag tttttcagcc tcgaccagag tacctttttg gtagccggta tcgataacga
     3241 aaatcgcgtc gggcaggcct ttcatgtttt tgataccgcc caaagaacgt tccagttttt
     3301 caacatcgcg ttgcatttcc agaatttctt ttttgctgaa accgctttcg gcagcatttt
     3361 ccaaggctgc ggttttttct tccaggcgtt tgatggattg cttaacggtt ttgtagttgg
     3421 tcagcatacc gcccaaccag cggtaatcaa cgaaaggcat accggcgcgg gtcgcttctt
     3481 cgcggatgat gtcgcgggct tggcgtttgg tacctacgaa caatactgta cctttgttgg
     3541 caaccaaacg acgtatggct tcttgcgcgt cttggaacat cggcagggtt ttttccaggt
     3601 tgacgatatg gattttgttg cgcgcaccga aaatgtattg tgccattttc gggttccaga
     3661 aacgggtttg gtggccgaag tggacaccgg cttcaatcat ctgacgcata gtaatttgag
     3721 acattttgat ttccttgaaa gggttaaggg cacacattca atcgcataga acttgatagg
     3781 cgacgcctat tgtcaagcac ccttcggcga aagtgggcat aagttttaaa aaataaaaga
     3841 gtgtttccga aatggaaaac tgacggatta tagcggataa gaggccgtct gaaaagggat
     3901 tcagggtttt gtgccgctgt ctttttgccg ttgtgcacgc tgctgttcga attctgcctg
     3961 ttcctgttgc ttcatcaagc ggttgcggcg gcggacggtt acgacgaaga ccgtgaacac
     4021 ggtgggcagc accgcccaaa aaaccaaata aatcaaggcg cgcgcaatac tcggctgcgc
     4081 ggcggaatac ataacggcaa caaaaagata gccgatggcg acgatgtgcc acattgtgtg
     4141 cgtcctgttt gaatgttaac aaaatcgtta taatcgcaac attctaccgc aatcctgaaa
     4201 ggccgtctga aatgaggaat gaggaaaaac gcgccctgcg ccgcgaattg cgcgggcggc
     4261 gttcgcaaat ggggcgggac gtgcgggcgg cggcaacggt aaaaattaac cgcctgctca
     4321 aacgttatat caagcacggt cggaaaatcg gcgtgtattg gccgatgggc aaggaattgc
     4381 gtttggacgg ctttgtccgc gcggcgcaaa aacgcggcgc aaaactttat ctgccttata
     4441 tcgaaccgcg ttcgcggcgg atgtggttta cgccgtatcc tgaaagcgga atggaacggg
     4501 agcgcatacg gggcagggcg aggttgaacg tgccgcagtt tgcagggcgc aaaatccgcg
     4561 tgcacggttt gtcggtattg ctcgttccgc ttgtcggcat agaccgcgaa ggctaccgtt
     4621 tggggcaggc aggcggctat tacgatgcga cgcttgcagc gatgaaatac cgtttgcagg
     4681 caaaaaccgt gggcgtgggc tttgcctgcc agttggtgga cagactgtcg gtcgaggcgc
     4741 acgacctgcc gctggacggt tttgtgtcgg aggcggggat attgtgtttt tagccgttca
     4801 gacggcattt tgaacggaaa tgccgtctga aggtggaata ttgggagaaa aggatgtcgg
     4861 aaaagataat actgccggtc ttgtcgttgg gtggcgtacg gctcgaaccg ttggatgtgc
     4921 atcatgaaac gggtttgcgc gaggcggttt gcgatgggga agtttggaag ctgggagtaa
     4981 cgtccgcgcc ccatccggat agggtggcag actatatcgg gacagcattg gcaacgcgtt
     5041 tggcgtttgc ggttgtcgat gaagaggcgg gcagggtggt cgggacaacg gcgtattatc
     5101 attttgaacc gcagattccg cgtttggata ttggattcac atggtatgcg gcatcggcac
     5161 ggcggacacg gataaacacc tgttgcaaaa tcatgctgtt ggattacgca tttgatgttt
     5221 tggcttgccg ttgcgtggga tggcgcacgg atattctgaa ccttgcctcg caacgtgcca
     5281 tcgaacggct gggtgcggaa aaagacggcg tgctgcgtat gcatatgctc cgtaaagacg
     5341 gcagtgtgcg cgatacggtc gtgtacagta tgctgaggga agattggtgc aaaaacaggg
     5401 aaatcctgac cggcaggttg gcagggtatg gcgtgcaggt gtgaggcagt attttca
//
LOCUS       DAXHPG010000140         5294 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXHPG010000140 DAXHPG010000000
VERSION     DAXHPG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.140"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            1..340
                     /locus_tag="ACFM6F_001736"
                     /pseudo
     CDS             1..340
                     /locus_tag="ACFM6F_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(361..1587)
                     /locus_tag="ACFM6F_001737"
     CDS             complement(361..1587)
                     /locus_tag="ACFM6F_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC6165114.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            1742..1928
                     /locus_tag="ACFM6F_001738"
                     /pseudo
     CDS             1742..1928
                     /locus_tag="ACFM6F_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            2132..2791
                     /gene="queC"
                     /locus_tag="ACFM6F_001739"
     CDS             2132..2791
                     /gene="queC"
                     /locus_tag="ACFM6F_001739"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC6165115.1"
                     /translation="MSNQQALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGIKQTVLDLSLMRQITHNALMDDTAAIETAGNGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2819..3334
                     /locus_tag="ACFM6F_001740"
     CDS             2819..3334
                     /locus_tag="ACFM6F_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC6165116.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAIPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSEMPKTEMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFTAATPAEAKQKALQTLLTDHVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            3341..3763
                     /gene="queD"
                     /locus_tag="ACFM6F_001741"
     CDS             3341..3763
                     /gene="queD"
                     /locus_tag="ACFM6F_001741"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC6165117.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDLVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4032..4403
                     /locus_tag="ACFM6F_001742"
     CDS             4032..4403
                     /locus_tag="ACFM6F_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC6165118.1"
                     /translation="MKLLSTLLVLLVTAEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4400..5038
                     /locus_tag="ACFM6F_001743"
     CDS             4400..5038
                     /locus_tag="ACFM6F_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC6165119.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGRLKAYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
BASE COUNT         1354 a         1535 c         1318 g         1087 t
ORIGIN      
        1 tgttttttcg atacggaacc ggatagagga aaaatcgaac attgcgcctg ccttgctatg
       61 attcacgaag aaatctccgc catgcctatg ggctatgaaa ccttgatcgg cgatatgggc
      121 agcgcactgt caggcggaca aaaacaacgc atcgtattgg cgcgggcctt aatattgcga
      181 accgaaaatc ctatttttag atgcagcgac cagccatttg gatattgcca atgaaaaagc
      241 agtcaatgca aacttgaatg gcttgtctat cataaaaatt atggcggcac acagaaagga
      301 aacggtggaa tcagcagata ggaaaatgtc tttaggataa aaatacagtt tcaaaaatac
      361 tcaagattgc tgctgttttt tcgcctgagc gtcaaactct gccagcgtca tgttcaaagt
      421 ctgcaaacac ggcatcatta ctgcatcgac agcttggttc acatgatccc tttccacagg
      481 caacggacgg taaacgaaga gcttgaagag ttcgttcaac tcaatcgaat ccgcccccgt
      541 tttcaacacc caaccctgtc tgccggaata gatatagccg taccgcgcca gcttttccaa
      601 aagctcgccc aactcgtcgt agcccatatt gatatgccgt ctgaactcct gaacaggcaa
      661 ggctttgcct tctttttgcg ccgcatccag aagcagcagg attttcaaca cgtcgtcaaa
      721 ccgtccgcgc gagtcgaagc ccctgcggaa cgcttctccc tgccagtagg agagtgaaga
      781 agtcagcacc gcgccgccca agaccagcgt ccacaacagg ttcagccaca acagaaaaaa
      841 cggcacggcg gcaaacgcgc cgtaaatcga gcggtagccg tcgaaattgc ccatatacca
      901 agtgaagagg gagcgcgcgg tttccagaca aaacgctgtt gccaaagccc cgacaaacgc
      961 ctgccgcgcg ggaacgaagc ggtttggcac gaagcggtac agcccccaca gcaaaagcgt
     1021 catgaaggtc agcgtcgccg ccgttcgcaa cgcgcccgac cactgcggcg cacctgaggc
     1081 aagcgcggca tcctgtaccg agccgaccat aaaggaaatg cccacgccca aagacagcgg
     1141 cccgaacgtc agtaaagccc aatagacgag aaactgcatc atccacggac gctgggaatt
     1201 gacccgccag atgcggttga acgtattgtc tatcgtccga atcagcatca gcgaggtaac
     1261 gaccagcatc acgctgccga ttgccgtcag ccggttcgcc tgctcgcgga acgcattgat
     1321 atagtcgaac accatgtccg cgccctgcgg cacaatggtt tggttgacga aggagacgaa
     1381 cgaatccgac cagcggtcga acacggggaa aatcgaagcg accgccacca tcacggtcag
     1441 cacggggacg agtgccagca gcgtcgtaaa cgtcatgctt gccgccgcct gcggtacgcg
     1501 ttcttcatca aagcggcgga cgacgaacca tgcaaacgca cagattttat tgtctgccaa
     1561 accttgcaaa cgttgtaaaa aggtcataat ttcttgcccg gtcagtaagt tgggcattga
     1621 tgcccgatgt tatagccaag tttgccgtca ggaacaaatg cctgaactgc ggctgtttca
     1681 gacggcatcg gaacaactgt tatgccgttt gaagaccgaa ccattttaac ggaatccgcc
     1741 catgaaccca aatccccctc aaaatcctcg tcctctacta ttcccaaaac ggcagccccc
     1801 gcaatcccgc ccgccgaatc gctcgcggca tcgacagcgt tgaaggttgc gaagccgtat
     1861 tgcgcaccgt ccccaaagtc tccgccgtct gcgaagccgt caaaaaagat attcccgaca
     1921 gcggctcccg tcctgaccgc cgaagaaaac aatatcgcct tcgcacaaag caaacgcttg
     1981 gcggaactcg ccgtcaagtc ggcataagcc gcgtgttcag acggcatggc gttcagatgc
     2041 cgtctgaacg cgtttgcctg tataatccgc atctttactg ttcaacttcg cggttcgcaa
     2101 acctcccgcg ttaccaaaac taggattcga tatgtcaaac caacaagcct tggtcatctt
     2161 ttcgggcggt caggattcga ccacctgcct gattcaggca atccaaacct acgggcgcga
     2221 aaacgtccaa gccatcactt tccaatacgg gcaacgccat gccgtcgagc tggaacgcgc
     2281 ccgctggatt gcgcaggatt tgggcatcaa acaaaccgta ctcgacttga gcctgatgcg
     2341 gcagattacg cacaatgccc tgatggacga taccgccgcc atcgaaaccg ccggcaacgg
     2401 tgttcccaat acctttgtgg acgggcgcaa cgcgcttttc ctgctttacg ccgcgattta
     2461 cgccaaaggg caggggatac ggcacatcat cgcgggcgtg tgcgaaaccg acttctccgg
     2521 ctatcccgac tgccgcgacg tgtttgtcaa atcgatgaac gttaccctta atttggcgat
     2581 ggactatgat tttcaaatcc acacgccgct gatgtatctg accaaggcgc aaacttgggc
     2641 gttggcggac gaaatgggcg cgctggacta tatccgcgag caaacccaca cctgctataa
     2701 cggcatcgtc ggcggctgcc gcgaatgccc gagctgtatc ttgcgcgaac gcgggctggc
     2761 ggaatatctg gaaagtaaaa aggccgtctg aacacgcgca aaccataagg aatacgatat
     2821 gcccaagctc catatgtttt acctcggcgg caatgccggc aggtcgaata tcgaagtgca
     2881 cgacatccaa tttgccgtgt gcgacgacta ccgcgaagcc atccccgcac tcaaagccgc
     2941 atggttcggc gatgcggaca aaatccacat cgacggttgg caggttgtcg aatgggcgga
     3001 cggttacgac atcgccgtat ccgaaatgcc caaaacggaa atgccgtctg aacacgcccc
     3061 gcgcctgtat tttgccaatg tcggcggtta tcgcgcgggg cagcttgccg aggcgcacgc
     3121 gttcgggctg ttcaccgccg ccacgcctgc cgaagccaaa caaaaagccc tgcaaaccct
     3181 gttgaccgac catgttcagc aacataaaga caacttaaaa gacgtggaca acctgcttgc
     3241 gctcgaccgc atcggcaatt tccatatccg cctgaccccg aatccgcacg gcaaacccgc
     3301 cgaaatcggc tttcaaggct atttgcccat ttgagaaccc atgaaaatca ccaaaatctt
     3361 caccttcgac tcctctcata tgctcgacgg gcatgacggc aaatgccaaa acctgcacgg
     3421 gcatacctac aaactcgaaa tcaccgtttc agacgacctc gtcagaggcg ggccgaaaga
     3481 cggtatggtg atggacttta ccgacttgaa agccattgtc aaacaacaca ttaccgaccc
     3541 cttcgaccac gccttcatct accacggcgg caacgggcgc gaatctcaaa tcgccgcact
     3601 cttggaaggc tggaacatga aaaccctgcg cctgccctgc cgcaccactg ccgaaaatat
     3661 ggcggtcgaa atgtacgacc gtctgaaaaa cgcggggctg aacgtgtgcc gcgtcaaatt
     3721 gtgggaaacg ccgacatcgt gtgcggagta tgaaggggag tagggaatat cttgaacgta
     3781 tcgatatagt aaattccaat aagacatgcc caaccgcgtc attcccgcgc aggcgggaat
     3841 ccagaccttg gtttttcagg aatatttaaa aattgcagca attccaaatc tttggattcc
     3901 cgcctgcgcg gaaaggacgg tttagagcgt ccttatttga atttaccgta aaacggtttt
     3961 ttctcctgta cggattcccc gttttttcag acgaccctcc atatcaaata cacccattaa
     4021 aaggaatacc catgaaactt ctctccaccc tcttagtcct ccttgtcacc gccgaacatt
     4081 tctacatcgc ctggcttgaa atgacgcaga ttcccagcga aaaagcggcg gaaatattca
     4141 agctgcctta tgaatttatg gaacaaaagc gcgtgcagac cctgttcggc aaccaagggc
     4201 tgtataacgg ctttctcggc atcgggctgg tgtggtcgcg gtttgccgct ccggataacg
     4261 cggtgtacgg cgcaacggta ctgtttctcg gcttcgtcct gattgccgcc gcgtggggcg
     4321 cgttctcttc cggcaacaaa ggcatactcg tcaaacaagg tttgcccgca tttttggcag
     4381 cggcggcggt gttggcggta tgaaaaaaat caatgttgcc cccgaaaatc cccaataccg
     4441 catcgtcgaa atctttgaaa gcctgcaagg cgaaggctgg aacacgggca tgcccgccgt
     4501 tttcgtccgc ttgggcaaat gcaacctcgc ctgcggctgg tgtgataccg attatttgac
     4561 attcggcatg atgagcctgt ccgacatctt aggccgtctg aaagcctatg ccgcccgcaa
     4621 catcatcatc accggcggcg aaccgaccat ccagccgcat ctcgatatgc tgctggacat
     4681 gctcaaggcg gaaggttatt tcctctgtat cgaaaccaac ggactcaatc ccgcgccgcc
     4741 gcaaatcgac tatgtcgcca ccagccccaa agcctgctac gccgccaaat atgaaaatag
     4801 ctgtatcgaa acagccgacg aagtgcggat tgttgccgat ggtgatgtcc ttgcgttctg
     4861 cgaaaacatg gaacgtaaaa tccgcgcgca tcattactac ctttcgccct gcgaacaaga
     4921 cggtgcgatg aacatctacg acaccatccg ccaaatcggt attttaaaca gtcgccccga
     4981 cgcgccggtg cattggcagt tgagcgtgca gacgcacaaa tgggcgggaa tagagtagtt
     5041 taagcagtgt aactcaaagg gccggcgtac cgttttgccg atgtctaaca tatgggaaaa
     5101 gtgtgccgct tccgcgtgaa actgcccaca tttccgctgc ctgattgagg cggaacctta
     5161 ctgaataaga tgccgccgtt gggtacaagc ccggcttcct aaattccgat ggtcttttga
     5221 accttgccga tactctgtgc cagtgcgcgc aaatggcagg gttagggaaa acgaaatgcc
     5281 gtctgaaaca gcat
//
LOCUS       DAXHPG010000141         5266 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXHPG010000141 DAXHPG010000000
VERSION     DAXHPG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.141"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            116..811
                     /gene="pgl"
                     /locus_tag="ACFM6F_001744"
     CDS             116..811
                     /gene="pgl"
                     /locus_tag="ACFM6F_001744"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC6165120.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            792..1778
                     /locus_tag="ACFM6F_001745"
     CDS             792..1778
                     /locus_tag="ACFM6F_001745"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC6165121.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1828..2676
                     /gene="hexR"
                     /locus_tag="ACFM6F_001746"
     CDS             1828..2676
                     /gene="hexR"
                     /locus_tag="ACFM6F_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC6165122.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2797..4443
                     /gene="pgi"
                     /locus_tag="ACFM6F_001747"
     CDS             2797..4443
                     /gene="pgi"
                     /locus_tag="ACFM6F_001747"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6165123.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETEPQKHDSSTERLINLYLQTNRK"
     gene            4457..5008
                     /locus_tag="ACFM6F_001748"
     CDS             4457..5008
                     /locus_tag="ACFM6F_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6165124.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTIQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKFSELACKTAETEARLEELH
                     NRKKALIDEMAREADKKELPKRL"
BASE COUNT         1343 a         1469 c         1391 g         1063 t
ORIGIN      
        1 aaaaaagcag tcccgatatt tggtatcaaa agcataaaca actgttcggc cgacatattg
       61 ctcaacccgt ttccataacg gaatatgccg tctgaaataa aaaaaggaca caaatatgtt
      121 tgtttggcac gaatacgaaa atgcggcaga agcggcgcag tctttggctg acgcagtggc
      181 ggatgctttg cagggcgcac tggacgagaa gggcggtgcg gtgttggcag tttccggcgg
      241 acgttcgccg attgcatttt tcaacgccct gtcgcaaaaa gatttggatt ggaaaaacgt
      301 cggcatcacc ttggcagatg aacgcatcgt gccgaccgtc cacgccgaca gcaataccgg
      361 tttggtgcgc gaatacctgt tgaagaacaa agcggaagcg gcaatgtgga ttcctatggt
      421 ggaagacgga aaaactgaaa ccgaattaca tcccgatgct gttgtcgatt atgcactgaa
      481 acattacaaa cagcccgatg ttttggtttt gggtatggga aacgacgggc atacggcttc
      541 gattttcccg aaagctccgc agtttcagac ggcaatcgac ggttcggcag gtgtcgcgct
      601 ggtgcatacc acgcccgtta ccgcgccgca cgagcgcgtc agtatgacct tggatgcgat
      661 tgcccatacg gggcatgtgt ttttggcgat acggggcgaa gagaaaaaag ccgtgttcga
      721 ccaagccgca caaggcgaaa accgcgaata tccgatcaac ctcgttttga accatcaagg
      781 agtgaactgc catgtcttct acgccgaata aacaagccgg atatccccga ctggtcgccg
      841 atatcggcgg gacgaatgca cgctttgcgc tggaaaccgc gccgcgcgtc attgaaaaag
      901 ccgccgtgct tccgtgtaaa gactacgata cggttaccga tgcggtgcgt gcctatctga
      961 atcaaagcgg tgcaacagcc gtacggcacg cggcatttgc catcgccaac ccgattttgg
     1021 gcgactgggt gcagatgacc aaccaccatt gggcgttttc catcgaaacc acccgtcaga
     1081 ctttggggct ggacaccctc atccttttga acgactttac cgcgcaggca ttggcggtaa
     1141 cgcagacttc aagcaaagac ctgatgcagg taggcgggca aaagcctgtc gaatttgccc
     1201 ccaaagccgt tatcggcccc ggtaccggcc tgggcgtgag cggattggtg cacagccacg
     1261 caggctgggt ggctttggcg ggcgagggcg ggcataccag tttcccgccg tttgacgata
     1321 tggaagtgct gatttggcag tacgccaaaa acaaatacgg ccatgtttcc gccgaacgct
     1381 ttttgagcgg cgcgggcttg agcttggttt acgaggcttt ggctgcaaaa cagaaagcca
     1441 aacccgccaa actgatgccg tctgaaatca cggaaaaggc tttgagcggc gcgtcgcctt
     1501 tgtgccgtca gactttggac atcttctgcg ccatgctcgg cacggttgct tccaacctcg
     1561 ccctgacgct gggcgcgcgc ggcggcgtgt acctgtgcgg cggcattatt ccccgcgtgt
     1621 tggaatattt caaaacttcc ccgttccgca gccgtttcga gaacaagggc aggtttgaag
     1681 catatcttgc cgcgattccc gtgtatgtcg tcttgagcga gtttcccgga atttccggtg
     1741 cggctgcggc tctcgacaac catttgagaa acgtttaacc acagcggctc cttgcagcgg
     1801 ggctgcatta tcgaagggca tatcattatg ttaagcaaaa tcagcgaatc actggcaaac
     1861 ctttccggtg cggaacgcaa agtcgccgaa tgtgcattgg cggaacccaa atggttcgtc
     1921 catgccgccg ttgccgaaat tgccgaacgt gcgtccgtca gccagccgac cgtcatccga
     1981 ttctgccgca gcttgggtta taaagggctg cccgagttca agctcgcctt gtccgccagc
     2041 atcggtcatg agggtatgcc ctatgtccac gaagaactca acgccgacga cgatatggca
     2101 agcgtggtcg agaaagtgtt gggcaatgcc gccgcctcgc tgttgggcga acgccgcttc
     2161 ctgaaagagt cggagctgga aaacgccatt gccacgctga tgcacgcccg tcgcgtcgag
     2221 ttttacggtg tcggcaattc cggcattgtg gcacaggacg cgcagcataa atttttccgt
     2281 ttcggcatgt ccaccgtcgc ctatgtcgat acgcacacgc agctgatggc ggcatctgtt
     2341 ttgagcgatc aggatgtttt ggttgccatt tccaacacgg gttcgtctat cgaacttttg
     2401 gatgcggtca gcatcgccaa agaaaacggc gcgtctgtca tcgcactgac ccgcaacgat
     2461 tcgcctctgg cgcaacttgc cgactgcgtg ttgagcgttg ccacacagga aaatgccgaa
     2521 ctctacacgc ccatggtttc ccgcctcttg cagcttgccg tcatcgacat tctcgccatc
     2581 ggacttgccc tgcgcttggg cgatgctgcc agcctgcaat tgcagaaaag caaaaaaagc
     2641 atacacaaca agcacatcga ttacgacaaa gattgacctt cagacggcat cccacaaatg
     2701 ccgtctgaaa tgccgaacaa cggtcgtcgg cggcttgcgg cagtttccgg cagccttttc
     2761 atcccacaac aaaaaacctc attcaggagc atatagatga aacaccttca cgacttaccc
     2821 gcatggtcga aattgtggaa ccactttgac gacagcaaaa cattgcatat gcgcgaaatg
     2881 ttcgagcaag acccgcagcg tgcggaacgc tactggctgc aagtcggcgg actgacgctg
     2941 gactactcca aaaaccgcat caacgacgaa accatgtcgc ttttgttcga gcttgcccga
     3001 gaagcaggcg tgccggagcg gatgcggcag atgttccacg gcgaaaaaat caataccacc
     3061 gaaaaccgcg ccgtcctgca tgtcgccctt cgcaaccgca ccaattcgcc gattgtggtt
     3121 gacggtgaag atgtgatgcc caaagtcaac cgcgttttgc aacgtatggg cgaatttgca
     3181 cacgaagtcc gcagcggaag ctggctgggc tataccaacc aagtcattac cgacgttgtc
     3241 aacatcggca tcggcggatc ggatttgggc ccgctgatga tgtgtaccgc gctcaaacct
     3301 ttcggtcatc cgcgcctcaa tatgcacttc gtctccaacg tggacggctc gcaactgcgc
     3361 gacgtattgt ccaaagtcca ccccgaaacc acgctgttca tcatcgcctc caaaacattt
     3421 accacgcagg aaacgctgac caacgcgctg accgcgcgcg aatggttttt gaatcatgcg
     3481 ggcgacgaag aagccgttgc caaacacttc gccgccgttt ccaccaatca aaaagccgtc
     3541 gccgaattcg gcatcgacac cgccaatatg tttgaatttt gggattgggt cggcggtcgg
     3601 tacagcctgt ggtccgccat cggattgccg attatgctgt atctcggcga agaaaacttc
     3661 attgaaatgc tcaacggcgc gcacctgatg gaccaacact tcatcaacac accgctcgag
     3721 cgcaacctgc ccgtcattct cgccctcatc ggcatctggt atatcaacta ctacggcggc
     3781 ggcagccacg tcatcgcgcc ttacgaccaa catttgcacc gcctgcccaa attcatccag
     3841 cagctcgata tggaaagcaa cggcaaacag gttacgttgg acggcaaagc agtcggacac
     3901 gaaacctcgc cgattatctg gggcgaaacg ggcattaacg gccagcacgc cttcttccaa
     3961 ctgctgcacc aaggcacgca cattaccccc atcgacctga ttgcctcgct tgaaaaacgc
     4021 agcaacctgc ccggacacca cgaaatcctg cttgccaacg tcttcgccca agcagaagcc
     4081 tttatgcgcg gcaaaacccc cgacgaagtc cgcgccgaac tcaaagcgca gggtatggat
     4141 gaggcgcgca tcgaagagct ggtcccgcac aaaaccttct ccggcaaccg cccgaccaac
     4201 ctcattctca tggacaaggt caacccgcgc aatatgggca gcctgattgc catgtacgaa
     4261 cacaaaacct tcgtacaagg catcatttgg ggcatcaaca gcttcgacca gtggggcgtg
     4321 gaactcggca aacaactggc taaaaccatt ttgggcgaac tgaccggcga aaccgagccg
     4381 caaaagcacg acagttcgac cgaacgcctg atcaacctct acctgcagac caaccgcaaa
     4441 taaaacctgc ggaaaaatgc cgtctgaacg ccgaccgttt cagacggcat ttttatcgaa
     4501 caggaaaacc gtcggtaaac ttgcagagcg tgtgcaatcc cgatatgatg gtttgcataa
     4561 atttaaacat atatgttccg cagctatggc actgattaaa gagccgttgg acaaagcgaa
     4621 acaaaggaac gaagaacttg aagcggcaga agaagcggcg gcgcaggagg cattgggtcg
     4681 ggagcaggaa gtcgaccgcg tatccgaatg ggaagaacgc tacaagctgt cgcgcagcga
     4741 gttcgagcaa ttctggaaag gattgcctca aaccatacag aataagctgc aagcctcaca
     4801 gaaaacatgg aaaagcggga tggataaaat ctgtgccaac aatgcgaaag ctgaaggtga
     4861 aacgccaaac ggcataaaat tcagcgaact ggcatgcaaa acggcggaaa ccgaagcacg
     4921 cttggaagag ctgcacaacc gtaaaaaagc ccttatcgac gaaatggcca gggaagcgga
     4981 caagaaagaa ctgccaaagc ggctctgaac agcgcggttc aggcactgcc cgccgatatt
     5041 gccgaaaccg tcatgcccga gtaccgcaac tgacaaaacg gtttgaatgc caaatgcgcc
     5101 gacggcgacg aatacggcgt ggcacaatct gactgccgta ccagagaaat caatgcgaaa
     5161 accaaagaaa tccaaggtta tctgattgac tgaaacttgg atgcgggaat gtcggcggct
     5221 tttgcgtttt tgtcgttttt atagtgaatt aaatttaaac cgatac
//
LOCUS       DAXHPG010000142         5258 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXHPG010000142 DAXHPG010000000
VERSION     DAXHPG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.142"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(107..2941)
                     /gene="lbpA"
                     /locus_tag="ACFM6F_001749"
                     /gene_synonym="tbpA"
     CDS             complement(107..2941)
                     /gene="lbpA"
                     /locus_tag="ACFM6F_001749"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC6165125.1"
                     /translation="MNRKHTFPLTLTALAVAAAFPSYAANPETATPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTHDIQSNKTGQFFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEKNQPLSAEEEAMVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEADKFR
                     DKSGVYDGNDFRDGLYFVPNIEEWKGDTNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAVSRPEQLSHNAARISEHTG
                     EYTADGKDKYLLGKPEVIEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPDSTGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2938..5115)
                     /locus_tag="ACFM6F_001750"
     CDS             complement(2938..5115)
                     /locus_tag="ACFM6F_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6165126.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAKPSIETTPVNQPAVGAAMRLPRRNIAAFDKNGNEIPDSKQAEEYLPLKEE
                     DILFLDGTPKEQADKLKKEINGRYPNVTIYTSDLKDAVYKYEHVRAGYVYTRFGTDEI
                     EQNSGGKQVTHRLGYDGFVYYSGERPSQSLPSAGTVKYSGNWQYMTDVKRHRAGKAVG
                     IDNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKTLTGQLIKNQYVQKKTD
                     EKKPLTIYNITAKLDGNRFTGSAKVNPDLAKNPAGNERLFFHADADQRLEGGFFGDNG
                     EELAGRFISNDNSVFGVFAGKKTETANAADTKPALPSGKHTKILDSLKISVDEATDGH
                     ARKFAISSMPDFGHPDKLLVEGREIPLVNKEQIIKLADGRKMTVSACCDFLTYVKLGR
                     IKTDRPAAKPKAQDEDSEDEIGESEENEDEISDEEGTEDEVVEDEGSEEDEVSEGDNG
                     ENEEEIAEEETEEEVDEAEEEKVEEPEEELPAEEGNGGSGSILPTPEAPKGRDIDLFL
                     KGIRTAEADIPKTGKAHYTGTWEARIGVPDKKDDQPGTTFIQKDSYANQGAKAEFDVD
                     FGAKSLSGKLTEKNDTHPAVYIENGVIDGNGFHATARTRDNGIDLSGQGSTNPQRFEA
                     NNLLVTGGFYGPQAAELGGNIIDSDRKFGAVFGAKKDDKEATR"
BASE COUNT         1115 a         1396 c         1248 g         1499 t
ORIGIN      
        1 ggcggaaatc ttgtttatat tgaatcaaaa aaaacctgca ccttaatcag ttggcggttt
       61 agtccgactt ttggggtgca gatcaagctt tcagacggta tttcctttaa aacttcattt
      121 cgagcgcgag actgaagttc ctgcccggtg cggcatacct tccatagttg ctgtcgccgc
      181 cgtgccggtt tgccgtgctt tccgcagtct ggcgcaagga ttcccaagta acgtagcggt
      241 agttgccgat attgtagata gccgccctca aggtcagccg ttttttcaga ttcagatagg
      301 cggaaacgtc tgccgtcgac caagaagacg acgctctttt tgtcgaatat cgtttttgat
      361 cgcctgccag ataagcaagc tcgtcagggt ttttcccttt ggaataggtc agcataatgt
      421 ttgcccccca tttcccctcg ggctggtcgt atccgaaccc caaaacataa cgcgacggct
      481 gtaccgcatc caaagcatag ctgcggagag acaggcccgg tcggttggat accgatttcg
      541 gtttgatgcg gttgtacgcc aaagtcgtgt acaaaccttc gggcagtttg ccgtacacac
      601 cgttccagtc gatttttccc aatatattga tgccttggag cgacatattt tgggcattgt
      661 aataatcgcg tatatcaatc tctgtcaatc gtcctgttga atccggcaat ttggttttgt
      721 gatcggcaac ggcaatcata tcggtatagc ggttgcggaa gctgctgatt tccaaaaagc
      781 cgaaatcgcc cttccactgc aaaccgattt cgcggttggc tgccttttcc gatttcaggg
      841 cgggacgctg ccagcctttc ggataatcgt gataaatgtc tatcccgaaa agctcttgga
      901 atgagggcgt tctgaagccg ctggaggcgc ggtaagacaa agaaaaatgc cggttcggtt
      961 tgaacacgat gccgctgttc cacgaacggt caacataccg tccgctgcgg acgagttctt
     1021 ccgacgtggt gaagtttttc cggtcgtacc tgccgcccaa gctgaaatca aaatatttgc
     1081 cgattgaaaa acggtcgttc aaagaaatgt ggatattgct gccgttgatt tttcttggca
     1141 cgcatttgcg ggaacgcaaa gtttcgatgt agccgcagac cgatccttca atgacttcgg
     1201 gcttgcccaa aagatacttg tctttgccgt cggcggtgta ctcccccgtg tgttccgaaa
     1261 tccttgccgc attgtgggaa agctgttcgg ggcgggaaac tgctttggaa gcatcgtaac
     1321 cgaagcccaa agtcagatgg tgtttcgtcc atttgttttt cagcgatttc tcaaacgagg
     1381 cattcaaaac attgtgctgt tcgcggtagt ggaaacggtc gctgctgtcg taggaatacg
     1441 gtttgtccgc cgacgcgcgg caggatttgt ccacagcagg atacacggcg caattcagct
     1501 tcagcgtgtt gttgtcggtt gccacgccct gtttgtcaaa cgacaacacc gccttatccg
     1561 cccaattgtc agaatacgct tcgttttcat aacggtacag caaacccata cggcggcggc
     1621 ggtgatgttc gtcaataaat ttggtgcggg aatatttcaa acctatgccc ttgaccaaat
     1681 ttgtatcgcc cttccactct tctatattcg gcacaaaata caagccgtcg cggaaatcgt
     1741 tgccgtcgta caccccgctc ttgtctctaa acttgtccgc ctcgtccgta ccgtaatact
     1801 gtttttccgt catatcgcgg atgtcgtaac gctgtttggt atcctcaaac acgccgccga
     1861 cataatgcct gccgccgaag cggtagccca gcttggcaag ccaagagccg ctgcggtaat
     1921 ccatcggatc gggcaatatc ctgccgccgc ccgtgtaagc ttgggcggac aggttttcgt
     1981 ggcgcgcctg cgcctcccgc accatcgctt cttcttcagc acttaaaggc tgattttttt
     2041 caatacgttc ttttacccag cggttaagct ggcggttcaa atatatcccg tagcccgcca
     2101 attttgccac gggcttgagt gtattctcgc cctctactaa gaaaaattgc cctgttttat
     2161 tgctttgaat atcgtgtgtc tggcggaacg cgtccaaacg gtttatgccg tatgccaccc
     2221 cgtccgcaat atcgccgtgc gggcgcgttt cccgcccttg gcgttcggtt cggattaaca
     2281 gcccttccca accgtctttg ctgaaccccg cgccgagcga cttcataaat tggcggtttt
     2341 tactgccgta ggcggttttt gcctgtatcc cccagctttt gccgtctgaa atcaggtctg
     2401 ccgcctcttt ggtgcggaaa gcgaccgcgc cgccgagtgc gccgctgccg tgatcggacg
     2461 aaccggcacc tttgtcgatt tccaccgtgc tgatgttttc atattcgatt tcgttgattg
     2521 caccgctgcc gccgcgtccg ccgtatccgc tcaacgatcc ctgcacggta aacgcctgta
     2581 tttgggcaac accgtcgacc gaaaccgcca cacggttttt atccacgccg cgtatcgagt
     2641 agccgccgct cgcgccgttg ccctgttcga caaccgccac gcccggatcg tagcgcgtca
     2701 ggtcgcggat accgagtacc tgttctttgt tcaacgtttc cgacgttttg acgattttgc
     2761 ccaaaccggt cgcctctttc gatcgccgtc ccactttggc ggcacggacg gtaacctctt
     2821 tcaaggattg ggtctgcgcc gcatcaggtg tcgccgtttc agggtttgcc gcatatgacg
     2881 ggaaggcggc ggcaacagcc aaggcagtca gggtaagcgg gaatgtatgc tttctgttca
     2941 tcgtgttgcc tccttgtcat ctttcttcgc accgaatacc gcgccgaatt tccggtcgct
     3001 gtcgataata ttaccgccca attccgccgc ctgcggtcca taaaagccgc ctgttacaag
     3061 aagattgttg gcttcgaagc gctgcgggtt ggtcgaaccc tgcccggaaa ggtcaatacc
     3121 gttatcccga gtgcgtgctg tcgcgtggaa accgttgcca tcaatcacac cgttttcaat
     3181 ataaacagcg gggtgtgtat catttttttc tgtcaactta cctgaaagcg acttcgcgcc
     3241 gaaatcaacg tcaaattctg cttttgcccc ttgattcgca tagctatcct tttgaatgaa
     3301 cgtagtgcca ggctgatcgt ctttcttatc tggtacgccg atacgcgctt cccaagtgcc
     3361 ggtatagtgt gcttttccag tttttggaat gtcggcttcc gccgtgcgga tacctttcag
     3421 gaaaaggtcg atgtccctgc ctttaggggc ttccggagtg ggcaggatgc tgcctgaacc
     3481 gccgttgcct tcttctgccg gcaattcttc ttcgggttct tcaactttct cctcttcggc
     3541 ttcatcaact tcttcttcgg tttcttcttc ggcgatttct tcttcgtttt caccgttatc
     3601 accttcggaa acttcgtctt cttcgctgcc ttcatcttct acgacttcat cttccgtgcc
     3661 ttcttcatcg ctgatttcgt cttcgttttc ttcgctttca ccaatttcat cttcggaatc
     3721 ttcgtcctgt gcctttggtt tggcggcggg gcggtcggtt tttatccgtc cgagtttcac
     3781 ataggtcaga aaatcgcaac aagcactgac ggtcattttc ctgccgtcgg caagcttgat
     3841 gatttgttct ttgtttacca aaggaatttc acgcccttcg acaagaagtt tgtcgggatg
     3901 accaaaatcg ggcatagagg aaatggcaaa cttacgggca tggccatcag tcgcctcgtc
     3961 aacggaaatt tttagagaat ccaagatttt ggtgtgtttt ccagacggca gggcaggttt
     4021 tgtatctgct gcgtttgctg tctctgtttt tttgcctgcg aatacgccga atacgctgtt
     4081 gtcgttgctg ataaaccgtc cggcaagctc ttctccgtta tcgccgaaaa aaccgccctc
     4141 aagccgctga tcggcatcgg catggaaaaa caaacgctca ttaccggcag ggtttttcgc
     4201 taaatcagga ttgaccttgg cactgccggt aaagcggttg ccgtccaatt ttgcagtaat
     4261 gttgtaaatg gtcagtggtt tcttttcatc ggttttcttt tgcacatact gatttttaat
     4321 taattgacct gtcaaagttt tcttatcgaa atcaaccgta tatttagcag gatgtttttc
     4381 cctttcgtcg acatccttag ccgcataaga agttgcacca acatcgttac cataaaatgt
     4441 gatataaccc aaattgtcaa tgccaaccgc cttacctgct cgatgacgtt tgacatcggt
     4501 catatattgc cagtttccgg aatatttcac cgttcccgcg ctcggtaaag attgggaagg
     4561 acgttctcca gaataatata caaaaccatc ataacctaag cggtgggtaa cctgcttacc
     4621 acctgagttc tgttcgattt catctgttcc aaatctagta taaacatatc cggcccggac
     4681 atgttcatat ttatacacag catcttttaa atcggacgtg tagattgtta cattaggata
     4741 ccgtccgttg atttcctttt taagtttatc agcctgttct ttcggcgtac cgtctaaaaa
     4801 caggatatcc tcctctttga gcggcagata ctcctctgcc tgcttactat cgggaatttc
     4861 attaccattt ttatcaaaag ctgcgatatt ccgccttggc agccgcattg ccgcaccgac
     4921 ggcaggttga ttgaccggcg tggtttctat agaaggtttg gcagggggcg aagtgggaac
     4981 gtccttagac ttgaaagtga cggggtacgc ggtcggcgtt gattcgacaa caggctgcac
     5041 gccgaaattg ccgccgatgc aagatgccaa aagtaagggc aacaagacaa tgccgccata
     5101 attcggttta cacatcccta cttttcctcc tatttgatta ataataatta tcattatatt
     5161 aatatgtaca gataatatca acccgttttt atagtgaatt aacaaaaatc aggacaaggc
     5221 gacgagccgc agacagtaca gatacattcc gtcattcc
//
LOCUS       DAXHPG010000143         5173 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXHPG010000143 DAXHPG010000000
VERSION     DAXHPG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.143"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(82..1338)
                     /locus_tag="ACFM6F_001751"
     CDS             complement(82..1338)
                     /locus_tag="ACFM6F_001751"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC6165127.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWNEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAIGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSVLPEENELQVERFLSEHPEFEPVNCAELLQSLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(1686..2027)
                     /locus_tag="ACFM6F_001752"
     CDS             complement(1686..2027)
                     /locus_tag="ACFM6F_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165128.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2231..3088)
                     /locus_tag="ACFM6F_001753"
     CDS             complement(2231..3088)
                     /locus_tag="ACFM6F_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC6165129.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3149..3964)
                     /locus_tag="ACFM6F_001754"
     CDS             complement(3149..3964)
                     /locus_tag="ACFM6F_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC6165130.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4028..4198
                     /locus_tag="ACFM6F_001755"
     CDS             4028..4198
                     /locus_tag="ACFM6F_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165131.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            4208..4993
                     /locus_tag="ACFM6F_001756"
     CDS             4208..4993
                     /locus_tag="ACFM6F_001756"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6165132.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            complement(4974..>5173)
                     /locus_tag="ACFM6F_001757"
     CDS             complement(4974..>5173)
                     /locus_tag="ACFM6F_001757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165133.1"
                     /translation="IVQGSPFNQIWVSIFGSFGHLAVSTFLPPLPVKMPSEISARYFR
                     RHTRPFSRIPGSGLKFKRVRK"
BASE COUNT         1159 a         1206 c         1415 g         1393 t
ORIGIN      
        1 tttttgttaa tccactataa ttgatgaaat gccgtctgaa cgctttgaaa agggttcgga
       61 cggcattttg ttcaaaccgg tttatttgcg ttgcaatacg gcggcgaaga agccgtcggt
      121 ttggtgtcgg gcggaatcga ggcgcaggta ttttccggta tccaaatcaa ccttcaggct
      181 ttgcagcagt tcggcgcagt tgacgggttc aaattcggga tgttcggaca ggaaacgttc
      241 gacttgcagc tcgttttctt ccggcaacac gctgcaggtg gcgtacacca agcggccttg
      301 cggtttcacc agtttggatg cggcatcgag gatgctgtgt tgctgttcca aaaggttggc
      361 cacggtctcg gcggattggc ggtatttgag gtcgggattg cggcgcagcg tgcctaagcc
      421 ggagcagggc gcatccacta aaacgcggtc ggctttgcct gccagtcggg caatacgggt
      481 atcgtgttca ctgctgatgc gttcagggtg gatgttggtc agtccggcac gggtcatgcg
      541 cggtttgagg ttggcaaggc gtttttcggc gatgtcgaag gcgtagattc tgcctttgtt
      601 cgccatttgc gcgccgatgg cgagggtttt gccgcctgcg ccggcacaga aatcgacaat
      661 gatttcgccg cgttttgcgc ccaccaataa agcaagcagc tggctgcctt cgtcttggac
      721 ttccagtgtg ccgtccaaaa acagctcgtg tttgttaagc gcgattttgt ttttcaggcg
      781 gatgccccaa ggcgaataag gcgttgcctc cgcatcggca ctttcggctt gcaacaatgg
      841 cagcacttta tcgcgtttgc ctttcaaggt attgacgcgg atgtcgagcg gcgcggcttg
      901 gttgatgctg cgtccgaagg cgaggatttc ttcttcgttc caatgctgtt gcagttgttc
      961 gaccagccat tgcggcaatt ctgcggcggt attcagaccg tctgaaaact cggttttgcg
     1021 ggctttcaaa ttgccgagga actctgtttc ttcttcatca agcaggtctt tgatttggct
     1081 gatgttggtg cttctgccga gaaccagtgc ggcgagtgcg gctttgcgcg gctgcgcgtg
     1141 aggacgacgc agggcggtac tgattttttg atagtggcgc agcgcggcaa aggcagtttc
     1201 ggcgatttcg tggcggtctt ggcggccgag ttttttgtgt tcgcggaaat aggcggagag
     1261 gacggcatcg gcaggctgtt tgaaagtcag catttcagcc aaaactttgg cggtatggtc
     1321 gagttgtgcg gcgttcataa tgtaatgggt tctcttactg tgggttattc ataatcaatc
     1381 taacgtcctt aaaataacaa tagcatcttt atcattactt agtattctat ccgcaataag
     1441 aaagatgtgt taaaagtgcg caatcgggat ttgtctaaat ttctccgtta actacaaagg
     1501 cagcctgaaa aaagttctat tttcaggtcg cttgaacagc atccgatata atcacatcag
     1561 cgatttcatt ttgtaatcac tccctaaaac atgaggaaaa aaacaaaatt tattttcttc
     1621 gttcagtata tctatcctaa atatgccatc actccattga gtttttaatt tgtatttcgc
     1681 tcttgttaaa tgtttttctg tttgccgatt tcttttgcta atgtaaaaaa tactctacca
     1741 ctctcattgg ttaaagtttt caagtattca tcaacatcag taacattaat aacaactgtt
     1801 tcatttgtat gtttattaaa ttctaaatca ataataatat tcccattaac caatatttca
     1861 tatatatctt cttcttcagg gagcatagac aaacaataag cacttgaagc tgaaaactct
     1921 tttttcagat actgaagaag aaaagtattt ttaattaaaa tattattaaa tttatctaat
     1981 gattgcttga cttctttagt tgtccaattt ttaacattat tcttcattgt attctacctg
     2041 gggtcgtagt tattaaatta ccatacttat ttgtttggat cgtcataata ttagttggca
     2101 tgttgttgtt atatttatca tatccaatat ccttattaac tcggatgact ctttcataaa
     2161 ttgtatggga aatgagattt ccatcactaa gcctctcaat agatatacgc gttaatgttg
     2221 ggaaagggtt tcagacggcc ttcataatgc gttcgatttc gtgccaaaag ccgtccggcc
     2281 gacattccaa aacggcaatc aagccttggt cgatacggcg gatttcggtt gcgttcaatg
     2341 tttcggtttc ggcgacacgc gcaggcgcga ggtaggccat acggtcgagc agatggtagc
     2401 ggacttcttg gcgtttaaac ccgtattccg cccaatcttg aatatagatg ccgcgccaac
     2461 cgtatggatt aagcggtggc tcaaaagcat ggaaaccgtg tggaaaaaat ccgatgccgc
     2521 gcacgatgga aacgggtttt acgtcaagcg gcagaccgtg ttgccgcaag gttcggatgc
     2581 cgtctgaagt gtgcggcaga ccgagttggg cggtcagttt ggcggctttt cccaacagcg
     2641 tgtctttggg gtcgagtccg cgcatgtctt cgagcctgcc cgtgccgccg ccgtaatatt
     2701 tgcaggtcag ctcgatatgg tagggcttgc cgttaagcct taccacaaaa tccgccgcgc
     2761 cttgcgtatt gccgtccgaa ccggaaaccg tgaggttgtg cgcgagcagt tcggcgtgtg
     2821 gcgcgttggc aaaccaaaat gccagcagtt cttcggcgta aatgccgagg cggtgtccga
     2881 acggggcgcg tcgggtgagg tgttccgtca gcggcgcggg atcggcatcc aatgccaaaa
     2941 gatagcggaa accgcgttcc cccaatagtt cgcgcacgcc caattcgcaa ccgctttgcc
     3001 acaaaggcgg cgcagtcagc agcgaggcga ggtcgcggac gggttggctg gtgagcttcc
     3061 accataatgc gtctagggcg taattcatgc ttatccgaat atcagtcatg ccgtctgaaa
     3121 agctggaaat gttttcagac ggccttaatc agtctttttt atggatacga tggctgacgg
     3181 tattgaaaaa accgcgcagg atatcgatgt cttcggtttg cgtgttggcg cgtccgaaca
     3241 ggctctgcat acggcgcatc agacgctcgc cgttgcggcg gttgaaaaag ccgatgtcgt
     3301 tcatcacgct ttccatgtgg gcgaccatgc ctttgatttg ctcgtgggtc gcagcgtggt
     3361 cttcttgttg aagatgggtc atgggcgaac cggtttggct gaagatttcg tagcacacga
     3421 cctgcacggc ttgggcgagg ttgagcgaga aatagtcggg attgccgttg atggtcatca
     3481 gtcggttgca ggcttggact tcttcgatgc tcaagccgaa agtctcgttg ccgaaaacca
     3541 gtgccacttt ctcgcctcgg tttgcggtct gcagtaattc ggataccaaa tcgcgcgggg
     3601 tttgcagcgg cgcagtaatt tcgcggcggc ggctggtcag ggcgcaggcg atggtggtgt
     3661 cggcaagggc ttcgtccaaa gaagcggcaa tggtggcatt ttccaaaacg tctgccgcgc
     3721 cggaagcgag gatgaagctt tcttccggta atttaaacga ttgaggatgc tccgggtcaa
     3781 acacgggcgg gttttccgtc atcggcgttg ccatcagatt tggggcgacg atggtcagtt
     3841 tgtgcagacc cattgttttc atcgcgcgcg cggcagagcc gatgttggcg ggatggctgg
     3901 tgcgcgtgag gatgatgcgg atgttgtcca gataagcggg cagggcgggt ttgagagtag
     3961 tcatgttcgg ttttcggtcg gaatggaata gggtataatg cgccgtcttt ttcgttcgga
     4021 cggtcaaatg ccgtctgaaa tgttctttaa caacgtcgga aatttaacgt tgcgcctgaa
     4081 gcagacacgc gtcgggcggt attttacccg atttggcagg atttacccca accaaataaa
     4141 ctccgatttt gccgctccga aggactgcgc gtccgaaccg gcggtatgcc acccttaagg
     4201 aaatctgatg aatccgtttt tgaatacagc ctttaaagcc gcccgccgtg ccggtcagat
     4261 gatgatacgc gccgcgggca atctcgatgc cgtcaaaacc gacagcaaag cattcaacga
     4321 ttttgtttcc gatgttgacc gcaattccga aatcatcctg gttgaggctt tgaaagaagc
     4381 ctatccgcac cacaaaatca cttgcgaaga aagcggctcc cacggtaaag ccgcagccga
     4441 atacgaatgg attatcgatc cgctcgacgg cacgaccaac ttccttcacg gccatcccca
     4501 atacgccatc tctatggcac tcctgcacaa aggcgtgttg caagaagctt tggtgtacgc
     4561 ccccgaacgc aacgacgttt acatggcttc gcgcggcaaa ggcgcgttgc tcaatgaccg
     4621 ccgcatccgc gtttccaacc gcatcgaatt gaaccgctgc ctgatcggta ccggctttcc
     4681 tgttgtcgat caaagcatga tggacaagta tctggcgatt ttgaaagatt tcttggcaaa
     4741 aaccgccggc ggccgtcgtg aaggcgcggc ttctttggat ttgtgtgctg tggcgactgg
     4801 tcgttttgac ggctttttcg agttcaacct caaaccttgg gatattgccg caggcgcatt
     4861 gattgttcag gaagcgggcg gtatcgtaac cgatatgacg ggtgaagacg gctggctgga
     4921 aagcggcgat attgttgccg ccaatcccaa agtgctggcg caaatgttaa aaatcatttc
     4981 cgcacacgtt taaatttcag cccgctcccg ggaatgcggg aaaacgggcg ggtatgccgt
     5041 ctgaagtatc gcgccgatat ttcagacggc atttttactg gcagcggggg caggaaggtg
     5101 gaaacggcta aatgcccgaa actgccgaag atggaaaccc atatctgatt aaacggagaa
     5161 ccctgtacga ttt
//
LOCUS       DAXHPG010000144         5163 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXHPG010000144 DAXHPG010000000
VERSION     DAXHPG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.144"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(960..1205)
                     /locus_tag="ACFM6F_001758"
     CDS             complement(960..1205)
                     /locus_tag="ACFM6F_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165134.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            1317..1745
                     /locus_tag="ACFM6F_001759"
     CDS             1317..1745
                     /locus_tag="ACFM6F_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC6165135.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            1757..2233
                     /locus_tag="ACFM6F_001760"
     CDS             1757..2233
                     /locus_tag="ACFM6F_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC6165136.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(2583..3071)
                     /locus_tag="ACFM6F_001761"
     CDS             complement(2583..3071)
                     /locus_tag="ACFM6F_001761"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC6165137.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(3139..4194)
                     /gene="aroG"
                     /locus_tag="ACFM6F_001762"
     CDS             complement(3139..4194)
                     /gene="aroG"
                     /locus_tag="ACFM6F_001762"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC6165138.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            complement(4356..4583)
                     /locus_tag="ACFM6F_001763"
     CDS             complement(4356..4583)
                     /locus_tag="ACFM6F_001763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165139.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCPVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            4810..5118
                     /locus_tag="ACFM6F_001764"
     CDS             4810..5118
                     /locus_tag="ACFM6F_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165140.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMGASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTIYKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK
                     "
BASE COUNT         1304 a         1287 c         1214 g         1358 t
ORIGIN      
        1 cagtgtcatg agaaattttc ctttgtcaag tgttaaaatt ataatgatta tatactacat
       61 cacactacat cgcaatgaaa aatcaggaaa aacaaaaaac ctctccgccg tcattcccgc
      121 gcaggcggaa atctagaatt ttaatgcggc aagaatttat cggaaaaaac tgaagtttaa
      181 agacctagat tcccgcctgc gcgggaatga cggtttagaa atcacctgaa acgagaaaaa
      241 gaaccgaaac cggacaagtc ggattcccgc acgggcggga aggacggaat ttcagattgt
      301 tgtttttgat tttctgtttt gtgggaataa cgggattttg gatgatgtgc atttatcggg
      361 aaaactaaaa accctccgcc gtcattcccg tgaaagcggg aatctagaat ttcaatgccg
      421 caagaattta tcggggatgt ccggggttta aaggtctgga ttcccgcctg cacgggaatg
      481 acggtttaga aatcacctga aacgagaaaa agaaccgaaa ccagataagt cggattcccg
      541 cacgggaggg aaggacggga tttcaggtta ctgtttttga ttttctgttt tgtgggaata
      601 acgggatttt ggatgatgtg cattttcggg aaaactaaaa accctccgcc gtcattcccg
      661 cgaaagcggg aatctagaat tttaatgcgg caaaaattta tcggaaaaaa ccgaagttta
      721 aagacctaga ttcccgcctg cgcgggaatg acggtttaga aatcacccga aacgagaaaa
      781 agaaccgaaa ccgaacaagc cggattaccg cctgcgcggg aatgacggct caaaagttac
      841 gaaacgaaaa ataaccgaaa ccaaacaagt cggattcccg cgcgggcggg aatgacgata
      901 agcgtaattt caatctgcca tattgtcttt tcacttgcct gaaaacagat ttcagccacc
      961 tatccctgca tccggcggct gatgtagtcg gcaatcaaat cccgcttttc ccaaaaaaac
     1021 ttctgcattt tttgttcgga atacagtttg cggaccagag tgggcaaacc gctgtggacg
     1081 atggcggcga tttctttgtt gcctttttct tcctgcattg ccgtctgaaa atccataccc
     1141 ctgcctttca gatattctgc aaggcggttg cggatgtggg aattgagttc gatggatttg
     1201 agcattttgc ctccgttttg cggtaaggat ttattcgatt tgtttatccg cctgatttta
     1261 tcacgtttcc caagcggttt gcctgtacaa taccgctttt cggcagggtg tgggcgatga
     1321 tgttttcttg gttcaagctg tttcacttgt tttttgtcat ttcgtggttt gcagggctgt
     1381 tttacctgcc gaggattttc gtcaatatgg cgatgattga tgtgccgcgc ggcaatcccg
     1441 agtatgtgcg tctgtcgggc atggcggtgc ggctgtaccg ttttatgtcg ccgttgggct
     1501 tcggcgcggt cgtgttcggc gcggcgatac cgtttgccgc cggctggtgg ggcagcggct
     1561 gggtacacgt caaactgtgt ttgggcttga tgctcttggc ttaccagttg tattgcggcg
     1621 tgctgctgcg ccgttttcag gattacagca atgctttttc acaccgctgg taccgcgtgt
     1681 tcaacgaaat ccccgtgctg ctgatggttg ccgcgctgta tctggtcgtg ttcaaaccgt
     1741 tttgacggag catcttatgc cgatagaaat cgaacgccgt tttttgattg gaaacgacaa
     1801 ctggcggcaa tacgccgacg aaccgctgct gttgaggcag ggttatctgt ctgttgaaaa
     1861 agaacgcacc gtccgtgtcc gcattgccgg aaaacaggcg tggctgacgc tgaaaggcta
     1921 tatttcggaa atcagccgca gtgagtttga atacgaaatc ccgcttgccg atgcggaaaa
     1981 gatgatggaa acgatgtgcc cgtttaaaat ggaaaaatgg cgttatccgg tcagatgggg
     2041 cggcagcctg tttgaaatcg atgtttttct tggcgataat gcgcctttgg tcgtcgcgga
     2101 aatcgagttg cccgacgaga atgccgattt tgaccgtccg gactggctgg gacgggaaat
     2161 cactgccgac ggtatgttta ccaatgccta tttgagcagg catccgttct caagctggaa
     2221 aaatgccgtc tgaaacggct tcagacggca ttctccgaca accatcgcaa atccaaagac
     2281 agcctctata tccttatttc tgctttcttt gttccattat tcccaataaa tccccataac
     2341 ctaaaatctc gtcattcccg cgcaggcggg aatccggttc tttaaacttc ggttttttcc
     2401 gataaattgc tttagctttt cgtttccgga ttcccgcttt tgcgggaatg acgatggaag
     2461 attgttgtta tttcggataa attaccctaa ctttaaaatc ccggattccc atcttcgggg
     2521 gaataacaat attttctatt tcagcccatc atttgcgggg gattttatag agagtttata
     2581 tttcaatatc tcaaataatg cacaaaagca tagcgcgtgc ctttgctgct gacacggcgt
     2641 tccgtccgct ctgcttcttt ccaatgcgtc cggtctattg cggggaaaaa tgcatctcct
     2701 tccacagaca aatccacttc ggttatccgc aaatcggtcg ccaatggcat cgcttgtccg
     2761 tatatctgcg cgccgcccat aatgacggct tcttccgcgc ctgcgcacaa tgccaatgcc
     2821 gcctccaaac ttgccgccgt ttccgcgcct gccgcgcaat aatccgcctg ccggctgatg
     2881 acgatgttcc tccgtccggg caggggtttg acgggcaggg attcccacgt tttccgcccc
     2941 ataatgacgg gtttgcccaa ggtataggct ttgaaaaatg cgaaatcttc ggggatgtgc
     3001 caaggcatag cattgcccgc cccgatgcac aggttttccg cacacgccgc aattagggtt
     3061 attttgagca tatttcctcc acaaaaatgc cgtctgaacg cttcagacgg cattttatga
     3121 cattccgtgt caaaaatctc aaccggcgcg cgccatacgt tttttgtttg cacctgccaa
     3181 caatgccaac agttcttcag tcgcgcccca accgatacac gcatcggtaa tgcttttgcc
     3241 gtacacttcc ggcttatcct gtctgccttc gaccaaatgg ctttccacca tcacgcccat
     3301 aatattgctc ccgtcctgtt ccaattgggc agcaatatct tgtgccactt ccatctgccg
     3361 ggtgtaatcc ttgcggctgt tggcgtggct gcaatctatc atcagcttat cggttacccc
     3421 tgccgcacgc agttgttccg ccgcctcgct gacgtgtccc gcatcataat tcggctcttt
     3481 tccgccgcgc aaaatgacat gacagtcggg attgccgccg gtatggacaa tggcggaatg
     3541 tccggccttg gttacagaca agaaatgatg cgaatggctc gccgcaccga ttgcgtcgat
     3601 ggcaatcttc aaattgccat ccgtaccgtt tttaaagccg acggggcagg acagcccgct
     3661 tgccaattcg cggtgaactt ggctttcggt cgtccgcgca ccgattgccc cccaagaaat
     3721 caagtccgca taatattgcg gcgtaatcat atccaaaaac tcggtagagg caggcatacc
     3781 catattgttc agcgacaaca acaggctgcg cgcctgacgc aaaccgaaat tgatatcgaa
     3841 cgtaccgtcc aaatgcgggt cgttaatcaa acctttccaa cccaccgtcg tcctcggctt
     3901 ctcgaaataa acgcgcatca caatcaaaag ctcgttttca tactgcttgc ggagtttcaa
     3961 caaacgctcc gcatattcca acgccgcttt cggatcgtga atcgaacacg gcccgataat
     4021 aaccaacagc cgcttgtccc tgccgtgaac caaatcggaa atttcctgac gggtgcggtg
     4081 aaccaagccc gaagcctctt tggaaatcgg cagctcgtaa agatgggcaa tcggcggcaa
     4141 caactcttta acttctttaa tcttaatatc gtctgtgggg taatggtgtg tcatcttgca
     4201 tctttcctga tggtttttta tacgcggctt aggttacatc gtttaccttg cacaaacaag
     4261 tgcaacatat tactacaaag ctattttatt tcaaaataac tgatatatct tttttcatag
     4321 attttaaatt tcatattttt aatattttag gaattttatt ccaaagaaaa agaaacagat
     4381 tgataaaaac aatacccgcc attcattcgg acaaaaccgc acggaacatc cgttttcccg
     4441 tttccacgac aatccggtac aggacacccc gctgccgatt ccgcctgcac atccggcaaa
     4501 atttcatacg caaggaaaat gttgaaaaca aaaagaacta attgcttgaa aaaagattta
     4561 attggaatgc gggtatttcc cacactttct ccatttattc atcctcatta taggaagaaa
     4621 aacatgaaaa aaccctgctt gcactctcac ttgcagccgt gtccgccgtt acggtagccg
     4681 atattcccga gtctaacggc aagggcaaag ccatcatcgg cgataaggaa aaacaccgtt
     4741 accaagcatg aaaaccttgc tttgaaagtc catggcgaaa tccgccaaga tttgggggac
     4801 ggatgatcct tgaccccttg ggcggatgca tatgcagatt tgcgcggcaa aaccaaagtg
     4861 atgacgaccc agatgggtgc ttcccgcgat gtcagcaaaa gcgccaaagg ttggagtgtc
     4921 ggtatcggtc tgaatgtagg caaacagttg accgacagcg tcggtctcga gtttgatcca
     4981 tactaccgtc acaaaacaat ctacaaaccc cgtgagattg tcttggacgg tgacaaaacc
     5041 aaaatgggcc gctccaaatc caacgagtac ggcttccgcg tagccgcaac gttctatagt
     5101 caattaaaat caaaatagga cagtagtgca tcgtcaaatc gggcgtaatc agacaaaacg
     5161 gtt
//
LOCUS       DAXHPG010000145         4999 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXHPG010000145 DAXHPG010000000
VERSION     DAXHPG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.145"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(32..673)
                     /locus_tag="ACFM6F_001765"
     CDS             complement(32..673)
                     /locus_tag="ACFM6F_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC6165141.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLNDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(663..1214)
                     /locus_tag="ACFM6F_001766"
     CDS             complement(663..1214)
                     /locus_tag="ACFM6F_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC6165142.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIDSDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDINTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(1218..1736)
                     /locus_tag="ACFM6F_001767"
     CDS             complement(1218..1736)
                     /locus_tag="ACFM6F_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC6165143.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDNTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(1736..3397)
                     /gene="pqiB"
                     /locus_tag="ACFM6F_001768"
     CDS             complement(1736..3397)
                     /gene="pqiB"
                     /locus_tag="ACFM6F_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC6165144.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFGKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(3390..4724)
                     /locus_tag="ACFM6F_001769"
     CDS             complement(3390..4724)
                     /locus_tag="ACFM6F_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HFC6165145.1"
                     /translation="MPPLIFATFPMKPFAENIPHSLRGNCCDEALPPHTVDCPECGCR
                     ADVPRLDSGEAAFCPRCGHKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGPAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1132 a         1259 c         1407 g         1201 t
ORIGIN      
        1 ataaacaact tgggtgcgga taaacctttg tttatctaaa cccctgtttt ctcaaaaatt
       61 gattcaaatc ttcggcaaat ttccgatagc ctttgccgtt ggcgtggatt tggtcggatt
      121 tcagattatt atcgcccaaa atttccgccc acgcgccgcc gaacagcgga atgccgtatt
      181 cctcggacaa atcctcatac agcggatgat cgttcaaatg cccgaacaac gcgcccaagg
      241 taatgtgcgg cacgccgacg aggacggcgg ggatgttttc cttctgcacg gtttcgatga
      301 ttttcgcgat attggcgcgg gtctgctcct tgggaacttt gcgcagaaag tcgttgccgc
      361 ctatgccgac aatcacaagc ttgggtttgc gtgccaacag cgcgggcagg cgcgacaggg
      421 cttgggcgga tgtatcgccc gatacgccgc cgttgacaat attccaaccc gtcagttttt
      481 gcagttgcgc ggggtaggat tcgccagggt ttgcgccgta gccgaaggta agcgaatcgc
      541 ccaaggcaag tacggtgctt ccttcgggaa ttttggcgtg ggttcgggcg gattttctgc
      601 cgcaggcggt aaggagcaac gcgcctgcgc cgaggaggaa ggttcttctg ttcattggtt
      661 tttcagatgg catcgagcca tttcgggtgg tatttcaggg tttcacggta aaccggacag
      721 ctttcggcgt gcgcgccttt gaggtagccg gtactcatca gaaattcgcc gacgatttcg
      781 ccgccgacga atttgaaatg ttttttaaag agtttaaccc attcgtcttt gcttcgcgga
      841 tggtgcgcgt cgagccagtt cttgaacgag ccgtattctt gttgcaacgc ttggatttgc
      901 cgtgcattga aaatggcggc atcgattttc aggcggttgc ggacgatgcc cgcgtcggca
      961 agcaggcgtt cgcggtcggt gtcgtcgaag gcggcaacgg tattgatgtc gaaaccttca
     1021 aatgccgtct gaaacgcctg ccgcttcttc agcatcagcg tccagcttaa tcctgcctga
     1081 ttgatttcca acaccagccg ttcaaacagt ccgttgtcgc tgtcaatcgg aaaaccgtat
     1141 tgcgtgtcgt ggtaatgttt gttcgggtta tcggtgtttt cgggaagtga ggcggcaaat
     1201 tcgcaatagt tcacggttta ctcgaccatc tgttgcgccg cctgtttcag tccctgttcg
     1261 agtgcggcgg tcatcgcggc gtagccgtca ccctgctgtt cggtttcgat atggaagggt
     1321 ctgttcgtac cgtcgggtag gacggcgtag ccgctgatga gggttttgcc cgtgtagctg
     1381 ccttggaatg cgtcgatata gaccgtccat ttttcggtac tgccgctgcg tgaggcagga
     1441 acaaagatgc gtgtgttgtc caaacggttg aatgcattgc tcaacgccgc ttcgagcata
     1501 tcgtccaagg tgtctgccca aacgtggttt tgtgcggtgt tgaggcggta ggggtcggtt
     1561 tgatagacca gtccgccgcg tttgagcggt tcggcaagac ggacttcgac ggcggtttcg
     1621 ccgccttgtg ttgcaggacg gatgtagcgg ctgtcgggca acacgaaata ttgtgtgctt
     1681 tgcacagtac cgcaggcggc aagcgtcagg gcggcggcaa tcgggaagag gcgcattatc
     1741 ggcttccttt cgggatgggg tctttgctgc tgctgttgaa aatcagcgcg ttgggttttt
     1801 ctttcaaagt gttaatgacg ggttgaacgt cttttaaggt tttgtccaaa ctttgcaacg
     1861 tattttgtac gtcgccgtag ataggcgatt gcggcgatac gccttgcagg gttgtgcgca
     1921 actctttcag ggtttggttc agttcgttcg gaatgttttg tgtctgcggt ttgccgacca
     1981 gtttgtcgat ggagcttagg gcggcattgg cagatttgag tgtggatttg agctcggcaa
     2041 gcgaaccgtt caattcggca accgtcttat ctaaaggcag tttgccgaat ttgtccagca
     2101 aatccgccaa tttgacctgc aaatcgtcca aaccgccgcc ctgggtcgcg ataacggtat
     2161 cgcctgcata aacggtatgc ggtcgcagct taggtgatgc ggaaggctga tcgttcaact
     2221 caatcatttt gcttccggtc agcaggttgt tgctggagat ggtggcggtc aggcctttgt
     2281 ttaaggccgt ctgaaattgt tgtttccaat gttctttgct ttgttcgtcg gcattgattt
     2341 ccaaacggga aggttcaatg cggatgcgta cgggtatcca gccgttttca aacaggtgca
     2401 ggctgtcgtt gcggtcgaaa taaggaacgt cggaaaccac gccgacattc agccctttgt
     2461 actcgacggg cgaaccgacg gtcaggccgc gcacggattg tttgaaaaac gcggtgtagt
     2521 acagcgagcg gtcgtcaggc aggttggcga cttcgctgcg gctgtcgtaa agcgtgaagc
     2581 tgtcttcgct tttgacgttt ttactgtttt tggttttcgg cgaatcaaat gaaatcgcgc
     2641 ccgacagcag ggcaggcaga ggggcggaat tgagtttgat gccgctgcct gtggtttcga
     2701 tattgatgcc gctttccagc cagaaacggc tggcggaatg aatcagtttg tcgttggggc
     2761 tttggatgaa gatggtgtaa tgcacgcttt ggtcggacgg gtcgaaatgc gcgctttcga
     2821 cttgcccgac cataaagttt tcatacaaaa cagggctgtt gacgttgagg atgcggtcgt
     2881 ttttaccaat caaattcaag cgcagcccgc tttgcccgat ggcggtaacg ggcggaatgt
     2941 cctgcacttg gaacacgtct tttgcctcgt cgcttttgcc gggtgtaaag gcgatgtacg
     3001 aacccgaaag cagcgtaccc aaaccggtta cgccgctttg gtcgatacgc ggcttgacca
     3061 cccaaaactg ggtatcgctg cggatgaggc cggatacgtc cgcattgagt tgggcggtta
     3121 cttccacgcc tttttggtcg tcgcgcagtt tgattcgggt aacgcgtccg acatcgatgc
     3181 tcaatacttt gatgaccgta ttgttgacct caatgccttc cgcgctgtcc atcaagagcg
     3241 taaccacagg ccccctgttg cggatttcct taacccaaag ccagccgccg gcaatcagcg
     3301 cgatcagcgg aaccagccag acggcagaga ggaaggtgtt gtttttgcgg acgcgtgctt
     3361 gggcgtgtcc gtttggagga gggctgttgt cagtcatgtt tttccgtttc attgaaagca
     3421 atgccgtctg aagcgcgttt gtcccaaagc aggcgcgggt cgaaataata ggcggacagc
     3481 atcgtcagaa tcacgaccag gcagaaatag actgccgcac tgcccggaat gacgcgcgcg
     3541 gcataagtgt ggaacgaaca catcaaaata ataatcacaa aaatatcaat catcgaccag
     3601 cggccgaccg cttcggtgat gcggtagagg tgcgacaatt tctttgcacc cgttggcaaa
     3661 gcgaagcggg cggacgcaat caaaaccgac attgccgcaa tcttcagtac cggcaccaaa
     3721 atactcgcgc tgaaaataac cgccgcaatc agcctgtcgc cctcgtccca catataagcg
     3781 atgccgttaa ggatggtatt gacctccgtg gcggcaggat tggacgaaat cataatcggc
     3841 aggatattgg cagggaaata caaaataacc gccgccgtca gaaacgccga cgaaatactc
     3901 agacttttcg gccgtcggcg gtacagttcc gcaccgcaca cgccgcaggg ggattcggca
     3961 ctgtcgcgga aatacaggca gcggctgcaa caggttttac cttccgatgc cgtctgaacc
     4021 gcattatccc ccgtcagccg cccgatttga aaatacaccc aatgctgggg aaccgatacc
     4081 gaagtccgaa tcagcataac cgacagcgcg aacatcagat aaaacgccgg cccgaagcga
     4141 acctctgcca cagacgagag cttgatatac gccaccaaag tggaaacaaa aaacacatcc
     4201 accatcatcg cctgtctcaa gcgcaccatc acacgcgttg ccaaacgcag cgcaggatac
     4261 gcctgtttcc gtatcagcgc ggcatagaca tacaggcaca gcagcagaaa cagaaccggc
     4321 gcgccgaaag tcagcacaaa catcacttcg gccaaaaaac cataatcctg aaacaccatc
     4381 aggcgcatca tctcgggcag cgaaaggacg gatgccgcac ccggtatccc gacctcgata
     4441 tacgtcatac cgtaagcaaa cgccattaaa atcagcgaag ccgccgcata ggcgggcggg
     4501 gcggaaaaag gatgcctgcc caccctgaag agtttgtgtc cgcaacgggg acagaacgcc
     4561 gcttctccac tgtccaaccg gggtacatcc gcgcggcagc cgcattccgg acaatctacc
     4621 gtatgcggcg gcagggcttc gtcgcagcag ttgccgcgaa ggctgtgggg gatattttcg
     4681 gcaaacggct tcatcgggaa agtggcaaat ataagtggcg gcattataaa ggaaaaaagg
     4741 cgatacaccc aatcaggatg caaaaccggc aggttcggca gtatgaacaa acccttggtg
     4801 tccggcaaaa atattttttg agcggttgtc cgccgactgc cgaacccatt tgataaaatc
     4861 ccgattctgt aaagaatagt aaaatacctg cttaaaaaca tcacgcaaat gatatttttt
     4921 ccaaaaatat gtgataataa atttcgttta tgattcttta ttatttttat agtggattaa
     4981 caaaaatcag gacaaggcg
//
LOCUS       DAXHPG010000146         4971 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXHPG010000146 DAXHPG010000000
VERSION     DAXHPG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.146"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(200..2476)
                     /gene="znuD"
                     /locus_tag="ACFM6F_001770"
                     /gene_synonym="tdfJ"
     CDS             complement(200..2476)
                     /gene="znuD"
                     /locus_tag="ACFM6F_001770"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC6165146.1"
                     /translation="MAQTTLKPIVLSILLISTPILAQAHETEQSVDLDEVTVVGKSRP
                     RATSGLLHTSTASDKILSGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            2898..3488
                     /locus_tag="ACFM6F_001771"
     CDS             2898..3488
                     /locus_tag="ACFM6F_001771"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC6165147.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRDNLPDCLEITAWTDDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3551..4609
                     /gene="trpD"
                     /locus_tag="ACFM6F_001772"
     CDS             3551..4609
                     /gene="trpD"
                     /locus_tag="ACFM6F_001772"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC6165148.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4666..4971)
                     /locus_tag="ACFM6F_001773"
                     /pseudo
     CDS             complement(4666..4971)
                     /locus_tag="ACFM6F_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684347.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1137 a         1141 c         1410 g         1283 t
ORIGIN      
        1 gatcttgggt ttctgctttt gatttttctg cttttgcgag aatgacggcg tgaaagtaag
       61 aatgatgaaa caaaaaaaat gggaatgatg gcatagtggt ttgttctttg tctttgccat
      121 atttcctaac aaattgatta aaaagaaaaa aggttttcag gatgccgtct gaaaaccttt
      181 tttgtttgcc tgtccgattt taaaacttca cgttcacgcc gccggtaaag ctgcggccca
      241 tttgcggcgt atcagagaga aagctgctgt gggcgtaaac ggattggttg agcaggttgt
      301 cggctttgac gtaccaattc cactcgccat agcgcgtatt gcggcggtag tttgcgccga
      361 ggttgagcat atggtgtccg ggcgtgcgcg tttcgtagcg ggcgagtttg ttttgggcga
      421 acacgcggta gtagtccaaa ttggcatcga tacggtcggt cagcgaggct ttcaggtgga
      481 agccgaggcg cgcagccgga acacgggggg cattttggtc gtcctgtgcg atgaaaggac
      541 ggttgccgta ggcatcttct ctgccgggta gggaaggcag gtttttcaga cggcctcgta
      601 catagtcgcc ggaaacgccg atgcggtagc gcggtgtcgg tttgaagtag atttcgcctt
      661 ccgcgccgta gaagtcggcg ccggattggt tgtagcgcac gagcttcatt tcgctgtcgt
      721 cttcgatgga tttggggccg cgtccgtcgt ttaaggtttg ggcgtaaatg tagttaccga
      781 agcggttgcg gtagagtgcc agattgtatt gccagcggtc gccttcgtag cccagcgcga
      841 gttcgatatt gttggaacgc tctttgttga ggtgtttgtt gccgacttca aaggtgttgg
      901 tggcgacgtg tttgccgtgt gcgtacagct cttgcgttga cggcaggcgt tcctgatggg
      961 aggcggtcag gctgagtttg tgttgtggcg tgaaatacca gttgcccgaa agtgcgaatg
     1021 agcgggcggt ttggcggtgc gcgccgaggt cgggcagggg gtggttgtag tagttttccc
     1081 gatcaatcaa tgctttgtcg tactgaatgg aggctttttg tttttccacg cgtacgccgc
     1141 cttcaagcgt gaagttgtcc cagtttgcct gttctacacc gaaaaagctg taatgttgca
     1201 ctttgttgtc aagcagcatc ggttgtttaa ccgcttcgga tatggcagat aaagcactgg
     1261 atttttgttg taaatattgc acgccccagc tgcctttcag acgacctatg ggttggtggc
     1321 gcaactcgat gcgggcgttt tgcgtttggt tgttaaaaaa gttttcgact gcatcgcctg
     1381 ctttttcgtc gtggcggtag tcgttgcggt tcaggtgtac gcgcagggct tcaaaaccgg
     1441 ggaacggttg cttccattcg gcacggagtt cgtagcgttt gttgcgcagg tctatccacg
     1501 gtctgccgct gtgggtgtgt gcgtgtgcat catcgtcgtc gtggaagccg cagctcaagc
     1561 ccggattgtc gtaatcgatg tcttcttcgg tcaacaggtg cggataaagc tgtaaatagc
     1621 gtttgttaat caagctcttt tgccagatga tgtcggcgtg gcaatcatcg tattcgtggc
     1681 tgtgggcagg cagaccatat tggtcgcgac ggtcgctgta cgctacgccg ataaaacctt
     1741 tttcgccaac ccaagacagc ccgatgctgc ccgtttgcga atcggcgtgg ctgtcgggca
     1801 ggcgtttcag attgcggtaa cgcggtacgg cgtaatcccc cgatttgcgg tacagccctt
     1861 ccgtgtgcaa tacaaagttt ttgcccaaac cgatattgat gccgccggac gtgagttttt
     1921 ccagattgcc gctgctcaaa cgcaatccga gttcgcccga tacgccgttt tcaggcattt
     1981 tttcggggat tttgccatcg gcaacatcga ccagccccgc cacattgccc gagctgtaca
     2041 agagcgtaac cggcccgcgc aggatttcga cctgttgcga caaggcggta tctaccataa
     2101 tggcgtgatc gggcgaaaaa tccgccatat cgcccgtttc gccgtgatgg ttcaacactt
     2161 taatccgcct gcctgtttga ccgcgaataa cgggagcgga cgcgccgccg ccgtattgcg
     2221 aggcgtgaat gcccggcacg ccgtccaaag catcgcccaa gttgacggct ttttggcgca
     2281 aggtatcgcc ggagaggatt ttgtcggaag cggtcgaagt gtgcaacagc ccggatgtgg
     2341 cgcgcggacg gcttttgccg acgacggtaa cctcgtccaa gtcaacagac tgctcggttt
     2401 catgcgcttg ggcgaggatg ggggtgctga ttaaaagaat tgataaaaca atgggtttga
     2461 gtgtagtttg tgccattttg gtttctcgtc gcatttcaaa agtttgttat tatataacat
     2521 tacattcctt atatcataag attttgagaa cactcagagg gcataggcaa aagtttttca
     2581 aatgaaacgg ttgcggcatc gggcggtgtc catttgtatc cgccgtcctt cgggggcgcg
     2641 gttgatgttg acgcagattc cgctgtgttt gcccattatg ttcggctgcg ggataaccaa
     2701 aatttatgag tgcataaaaa cggtacgttc ccgaacggtc gaaaaggtgg caaaatggcg
     2761 tacttgtcag aacgcgaggc tctgcgccag gttcacgagg gtgcatcggg cacgctaaga
     2821 tataagaggg tatggatggg ggtatcggaa atgcagttaa taacaaacaa attataaatc
     2881 aataggttaa taacaaaatg cttttgttta tcgacaatta cgacagtttt acttacaaca
     2941 tcgtccagta tttcgctgaa ttggggcagg aagtcgccgt gcgccgcaac gatgatatta
     3001 cgttggaaga aatcgaggca ttgaatccgc aatatctcgt tatcggaccc ggtccgtgtt
     3061 cgcccaaaga agcggggatt tccgtggcgg cgatgcgcca ttttgccggc cggctgccga
     3121 ttatgggcgt gtgcctcggg catcagacga taggcgaggc gttcggcggc aggatagtcc
     3181 gcgccaaaac gctgatgcac ggtaaggtgt cgcccgtgtc ccattcgggc aagggtatgt
     3241 ttaaaggttt gcccaatccg gttacctgta cgcgttatca cagcctcgtt atcgatcggg
     3301 ataatttgcc cgattgtttg gaaatcacgg cttggactga cgacggcgag attatgggcg
     3361 tgcgccataa ggaatatgcc gtcgagggcg tgcagttcca ccccgaagcc ctcttgaccg
     3421 aacacggaca tgatatgttg aacaattttt taatcgaatt tcaaaacttc aaaccgcaaa
     3481 aaatctgacg cgatgccgtc tgaaggcttc agacggcatt ttcgtccgaa tattgaacgg
     3541 aggacaaaaa atgattacac cgcaacaggc tatcgaacga ttaatcagca ataacgagtt
     3601 gttttacgat gaaatgaccg acttgatgcg tcagattatg agaggacagg ttctgccgga
     3661 gcagatagcg gccattttga caggattgcg tatcaaggtt gaaaccgttt ccgaaattac
     3721 cgcagctgca gccgtcatgc gcgagtttgc gacaaaagtg ccgctggaga atgcagaggg
     3781 gctggtcgat atcgtcggta cgggcgggga tggtgccaaa accttcaata tttcgacgac
     3841 ctcgatgttt gttgccgcag cggcaggcgc gaaggttgcc aaacacggcg ggcgttccgt
     3901 ttcatcttcc agcggcgcgg cggacgtgat ggagcagatg ggcgcaaacc tcaacctgac
     3961 tcccgaacag gttgcccaaa gtatcaggca gaccggcatc gggtttatgt tcgcgcccaa
     4021 tcaccacagt gccatgcgcc atgtcgcccc tgtacgccgt tcgctcggtt tccgaagtat
     4081 tttcaacata ttgggtccgt taacgaatcc tgcgggcgcg ccgaaccagc ttttgggcgt
     4141 gttccacacc gatttgtgcg gcattttgtc gcgggttttg caacaacttg gttccaaaca
     4201 cgttttggtc gtttgcgggg agggcggttt ggatgaaatt acactgacgg gcaaaacgcg
     4261 cgttgccgaa ctcaaagacg gaaaaatcag cgaatacgac atccgcccag aagatttcgg
     4321 catcgaaacc cgccgcaatt tggatgaaat caaagttgcc aatactcagg aatctttgtt
     4381 gaaaatgaat gaggtgctgg aaggaagaga aggggcggca cgcgatatcg tattgctcaa
     4441 caccgccgcc gccctgtatg ccggaaatgt cgctgcttcg ctttcagacg gcatatctgc
     4501 cgcacgggaa gccatcgatt caggcagggc caaatcgaaa aaagaggagt ttgtcggttt
     4561 tcaaccacaa caaagatgcc attttcttgg aaagatggag cttgggtgat gccgccatga
     4621 ttatggaact tttgtggcaa aacataagca cttcacgaag agaacttacc aaactgtttt
     4681 tatataaaaa cttggggctg tactagataa ccagaccaaa ttcccattaa ctaattgtct
     4741 taaaatctga atttgagatt ctatttaaaa tgccattggc atttctttaa atgcagcccc
     4801 aaatgctctt tgggaatgcc gttaaactta cgtaaatggc taaattcact aatatcaagc
     4861 acatcataac tacgaaaggt atccgtgtat acaatgccat caggcttaac tttctttcgg
     4921 ataattggca acaatgtcgc tgattgcgca ttaggaacaa cgacggtata a
//
LOCUS       DAXHPG010000147         4885 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXHPG010000147 DAXHPG010000000
VERSION     DAXHPG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.147"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            433..957
                     /gene="nspA"
                     /locus_tag="ACFM6F_001774"
     CDS             433..957
                     /gene="nspA"
                     /locus_tag="ACFM6F_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC6165149.1"
                     /translation="MKKALATLIALAIPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            1093..1482
                     /locus_tag="ACFM6F_001775"
     CDS             1093..1482
                     /locus_tag="ACFM6F_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6165150.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1560..2390
                     /locus_tag="ACFM6F_001776"
     CDS             1560..2390
                     /locus_tag="ACFM6F_001776"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC6165151.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2405..3862
                     /locus_tag="ACFM6F_001777"
     CDS             2405..3862
                     /locus_tag="ACFM6F_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC6165152.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3903..4292)
                     /locus_tag="ACFM6F_001778"
     CDS             complement(3903..4292)
                     /locus_tag="ACFM6F_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165153.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(4474..4875)
                     /locus_tag="ACFM6F_001779"
     CDS             complement(4474..4875)
                     /locus_tag="ACFM6F_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165154.1"
                     /translation="MKIKPLQFSNNSRRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
BASE COUNT         1201 a         1498 c         1041 g         1145 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaaaa ccgtatgcct gcacctgcaa
      181 caatccgaca gataccgctg ttttttccaa accgtttgca agtttcaccc atccgccgcg
      241 tgatgccgcc accaccattt aaaggcaacg cgcgggttaa cggctttgcc gtcggcaaag
      301 cagccggata ccgctacgta tcttgaagta ttgaaaatat tacgatgcaa aaagaaaatt
      361 taagtataat acagcaggat tctttaacgg attcttaaca atttttctaa ctgaccataa
      421 aggaaccaaa atatgaaaaa agcacttgcc acactgattg ccctcgctat cccggccgcc
      481 gcactggcgg aaggcgcatc cggcttttac gtccaagccg atgccgcaca cgcaaaagcc
      541 tcaagctctt taggttctgc caaaggcttc agcccgcgca tctccgcagg ctaccgcatc
      601 aacgacctcc gcttcgccgt cgattacacg cgctacaaaa actataaagc cccatccacc
      661 gatttcaaac tttacagcat cggcgcgtcc gccatttatg acttcgacac ccaatcgccc
      721 gtcaaaccgt atctcggcgc gcgcttgagc ctcaaccgcg cctccgtcga cttgggcggc
      781 agcgacagct tcagccaaac ctccaccggc ctcggcgtat tggcgggcgt aagctatgcc
      841 gttaccccga atgtcgattt ggatgccggc taccgctaca actacatcgg caaagtcaac
      901 actgtcaaaa acgtccgttc cggcgaactg tccgccggtg tgcgcgtcaa attctgatat
      961 gcgccttatt ctgcaaaccg ccgagccttc ggcggttttg ttttctgcca ccgcaactac
     1021 acaagccggc ggttttgtac gataatcccg aatgctgcgg cttctgccgc cctatttttt
     1081 gaggaatccg aaatgtccaa aaccatcatc cacaccgaca aagcccccgc cgccatcggc
     1141 gcgtacagcc aagccgtccg agcaggcgac accgtttaca tgagcggtca aatccccctc
     1201 gatcccgcca cgatgaccgt cgtcggcaac ggcgatttcc gcgccgaagc gcgccaagtg
     1261 ttccaaaacc tgcaagccgt cgccgaagcg gcaggcggca cgctggccga catcgtcaaa
     1321 ctcaacgcct acctgaccga cttgggcaat tttgccgtct tcaacgaagt gatggcggaa
     1381 tttattgccg agcccttccc cgcccgcgcc gccgtcggcg ttgcctccct gccaaaaggc
     1441 gtgcaggtcg aagccgaagc cgtcctcgtt ttgaacgcat aattccacgt tcggactgca
     1501 tgatatttca aaaccatgcc gtccgaaccg caacactttc ccacttcaca acatctgcaa
     1561 tggcacatta cgcaatcggc gacatacaag gctgtttcga cgaactgacc gcgctgctcg
     1621 gcaaaatcgg tttcaaccac ggcacggaca ccctctggct gacgggcgac atcgtcaacc
     1681 gcggcccgaa atccctcgaa acgctgcaat tctgcatccg gcacgaaaac agcgtgcaaa
     1741 tcgtcctcgg caaccacgac ctgcacctgc tcgctgtcgg ctgcggcgaa ggcgcgccca
     1801 aacgcagcga cacaatcgaa cccatactca aacaccccga cggaaaaaaa atgctcgact
     1861 ggctgcgtgc gcaaccgctg ttgatacgcg agggcagccg cgtgatggta cacgccggca
     1921 tcctgccgca atggcgcatc gccaaagccg aatcgctcgc tagagaagcc gaagccgaac
     1981 tgcgcggcaa aaaatacgtc aaattcttct ccaaaatgta cggcaacaaa ccggccgcgt
     2041 gggacgaagg tttggaaggc tatgcccgcc tgcgcttcat cgtcaacgcc ttcacgcgga
     2101 tgcgcgccct gacctttaaa aacgaactgg atttcgacta caaatccaca gtgaaaaaaa
     2161 tgccgcctta cctgcgcccg tggttcaaag cccccgaccg gcaaaacctc gaccgcacca
     2221 tcatcttcgg acactggtcc tcgctgggct acacgaatgc cgacaacgtc atctcgctgg
     2281 acaccggcgc gctgtggggc ggacagctga ccgccgtcaa cctcgaaacg gaagaaatta
     2341 cccaagtcca agccgccggc ggcatagact ggaaaagctt cgcaaaataa cggaccgccc
     2401 cactatgcac aaaatcctgc ccatcgccca cgtcctctcc cgactgggta tgctgttttc
     2461 ctttatcctg ctgatacccg ccgccctctc ctacgccttt tcggacggcg cgtacaccgc
     2521 cttcgccacc accgcgaccg ttaccctttc cggctcgtgc atcgtccggc tcgccaccct
     2581 ccggttcagg cgcgaactgc gcccgcgcga cggcttcacc ctcgtcctga tgttgtggct
     2641 ggcgtttgcc gctatggcgg cgatgccgat gtacctgtat ttcccgaata tgggctttac
     2701 cgacgcattt tttgaatcga tgtcgggact gaccaccacc ggcgcgaccg tcatccccca
     2761 cgtcgacggg ctcgccccct ccgtcaactt ttggcggcat atgctcaact ggctgggcgg
     2821 gatgggcatc atcgtccttg ccgtcgccat cctgcctatg ctcggcgtag gcggcacgca
     2881 gttattcaaa gccgaaatcc ccggcattga caaagaaagc aaaatgtcgc cgcgcatttc
     2941 ccaagtggcg aaaaaacttt ggttcggcta caccctgatc accatcctcg cggcagcctg
     3001 cctgcatttt gccgggatgg gctggttcga tgccgtctgt cacgcgatgg cgaccctctc
     3061 gctgggcggg ttttccaccc acgatgccag catagcttat tacaactccc ccctcatcga
     3121 agcggtcatc atcgttttca ccattgtcgg cggcatcaat tttgccaacc atttcgccgc
     3181 cctcaacagc cgcagcctca aaacctattg gaaagacgaa gaatgccgga cgatgctgct
     3241 gctgctctcc ggcagcatcc ttgccgccgc cctgtacctg tggcacaccg gctattacgc
     3301 cggttttacc gaatccctgc gctacaccgc cttcaacttc gtctccatcg gactggcaaa
     3361 cgggctggcc aacaccgact tcgcacaatg gccgctcctg atttccctgt ggatgttttt
     3421 cctcgccaac atcctcgcca actccggctc gaccggcggc ggcatcaaaa ccatacgcgc
     3481 cctcgtcctg ttcaaattca gcctgcgcga aatgatggtg ctgctgcacc ccaaagccgt
     3541 ccgcaccgtc aaaatcagcg gcaaggccat acccgaccgc ctcgcgctga ccgttatgtc
     3601 cttcatcttc atctacttta tgaccgtcgt cctcttcagc ttcctgctga tggcgagcgg
     3661 tatggaattt accaccgcct tcaccgccgt catcgcctgc atcaccaacg ccggccccgg
     3721 cctgggcgaa gtcgggcccg ccggcaatta cgccggtttg gacgtgatgc agaaatggat
     3781 ctgcgttacc gccatgctct tgggcaggct ggaaatcttc accgtcttca tcctcttcac
     3841 ccccgcctac tggaagaaat aaaaaatgcc gtctgaaaac ttttcagacg gcatattgtc
     3901 ttttaaaaaa gcggaagctc tacaaaagga aagaaatgct gaaacctctg ataaatttca
     3961 ggatatttct ctaaggcttt taatgctttt tcttttgaaa taggcggatc atagacatct
     4021 atccccctta agaaggcaat gcccgttaat gcattttttt gatcatttga aatgtaacca
     4081 taattaacga ctccaattac atcataaatt tcccctatac tcttatcaaa atcaatcaaa
     4141 tcaaaaatat acaatggaat tgtatcaata ggagtatctc tgacaactat atccaaccat
     4201 aatttaaatt cagaaagatt aatagcatca tagaacatac agactattgc aaatcctaaa
     4261 tcttcacaat tttctttact aattttccac ataattattt acctttatcc attgcttccc
     4321 aaccgataca tccggtattt tagattctcc tttgccgtca aacgctcttt tggttacctg
     4381 ctttgtatca aaaatgccgt ctgaaagccg aataccgctt cagacggtat tttgattgtt
     4441 gggtttttaa tcagagcttt gtttgataat ttgttatttt gaataaaata caggttctgt
     4501 cgagctgata acttctatta cagaatcgct aaaaaagaaa atataagcct tataatattt
     4561 cttttcaaaa aaatcaaaac tttcttcttt caaccagtcc atataactgt catctattat
     4621 ctcgaaaaaa taatttatag caggatttct atcccaatca tttttttctt tattttcaaa
     4681 taaatattct gaaaatttta ctttataaga ttcatctgtt atttgaaaat gaattatatt
     4741 gtcaataaat atttcaatat ttaattcata atttcggtcg gatgccaaaa tgttcacatc
     4801 ttgttcggta acacaaattt tgtctacgta aaatctgcga ctattgttag aaaattgtaa
     4861 tggtttaatc ttcactatcg ttttc
//
LOCUS       DAXHPG010000148         4876 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXHPG010000148 DAXHPG010000000
VERSION     DAXHPG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.148"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(92..574)
                     /locus_tag="ACFM6F_001780"
     CDS             complement(92..574)
                     /locus_tag="ACFM6F_001780"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6165155.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(727..888)
                     /locus_tag="ACFM6F_001781"
     CDS             complement(727..888)
                     /locus_tag="ACFM6F_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165156.1"
                     /translation="MSGQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(962..1423)
                     /gene="recX"
                     /locus_tag="ACFM6F_001782"
     CDS             complement(962..1423)
                     /gene="recX"
                     /locus_tag="ACFM6F_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC6165157.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQTA
                     IDVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1498..2208)
                     /locus_tag="ACFM6F_001783"
     CDS             complement(1498..2208)
                     /locus_tag="ACFM6F_001783"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC6165158.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            2615..3880
                     /locus_tag="ACFM6F_001784"
     CDS             2615..3880
                     /locus_tag="ACFM6F_001784"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFC6165159.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     ASYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3937..4743)
                     /locus_tag="ACFM6F_001785"
     CDS             complement(3937..4743)
                     /locus_tag="ACFM6F_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC6165160.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1050 a         1107 c         1435 g         1284 t
ORIGIN      
        1 aatttaatcc actatatttg cttgatttca tccgtttgca atggaaacgg atggcgcgat
       61 gtgcctgtct ttcagacggc atagtccgcc gtcagcagcc gcaggacatg tcttcagagg
      121 cttgcaggct gatgtctctg cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg
      181 tcaggatttc cagcggaggg acagggacgg gtttgccgtc tttgaccgca accatcgtga
      241 agtagcagct gttggtgtgg cggatttcgc cggtgcggat gttttgtgct tcgacacgga
      301 tgccgatttc catcgaggtg cgtcccgtgt agttgacagc ggcgtaaaag gtaaccaaat
      361 cgccgatgtg gatgggttct ttaaacagga ctttgtcaac cgacagggta acgcaataat
      421 tgccgctgta acggctggcg caggaatagg cgacttggtc gagcaggagc aggagttcgc
      481 cgccgtgtac gttgccgctg aaattggcgg tgtccggcat catcagttcg gacataatga
      541 gttcgtggga aggcagttgg cgttgttgtg tcatggtgta cctgttttaa gaaagggtgt
      601 tttgaaatgg gtgcggggaa attttaggga atggggaagt ggaattcaag ctaaaaaaag
      661 ctatgatggg atagtctgcc gaaatggacc gccgatgccg tctgaagcct tcagacggca
      721 ttttgtttag gaaagccaaa aacggaagac caggaaaaac acgagtgcca atacggcgac
      781 ccaaacgacg agcatgaatc cggcaactgc ccgtcccata cggtaaaccg ggtttttgcg
      841 ttcgtcttcg atgggggagg tgtggtgttt ttcgggttgt ccggacattt tgttctcaat
      901 cggaaaagtt ggaaacgggc gtaaggttat gttgccgtgc aaaaagattc aagaaggaag
      961 gtcagcagcc gtcttcccaa ttttcgtccc aagcgtgttt caatgccgtc tgaacggtat
     1021 cggcatcgaa tccgcgatag gcgaggaaac gcgcctgttt ttgtttttct ttaaggtcgg
     1081 cggcggggtg tttgaatttt ttacgcaaca cgtctatggc ggtctgtttt tcgcttgagc
     1141 ggtcgggaag caggttgcgg ctggtttctt catctatgcc ctgttgcgcc aaagcctgtt
     1201 tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat ataggcttcg gcatagcgca
     1261 aatccgactg ccagttgcgt tcggcaaatt cgtttaacac gttttccaac tcttcttcgc
     1321 tttcggcgtg cggtgcaagt ttgcgtttca gaccgatgcg gctgacttct tggcgcgaga
     1381 ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt catggcggcg gtttcggcgt
     1441 ggggcaggtt tcagacggca tgggaaacgg attccgatgc cgtctgaaca gattgtttta
     1501 ttcttcgtct ttatttttct gcggttgcag gttttcgtaa atttcgggca gcgagccgat
     1561 aatccagtcg gggcgggtcg catcgtcttg cgagagcagc gtcatatcgc cgtaaccgaa
     1621 ggtaacgccg acgctcaggc agccggcggc tttggcggcg atgatgtcgt tgcgcgagtc
     1681 gccgaccata accatgtttg caacatcgat acccaaaact tcggcggcat gccgcagcgg
     1741 cagggggctg ggttttttct cgggcaggct gtcgccgccg agtatcaggc tgaagtagtc
     1801 ggcaagtccg agctgtttca acagttcgac ggcaaggatt tcgtttttgt tggtaatgac
     1861 tgccagcggg atgcccaaag atttgagcaa tgccagcccg gcttcggttt cgggataggg
     1921 gcgggtgaag acgctcaaat ggtcgcggta gtatttcata taggatacga aacctttttc
     1981 ccacagttcg gaatcggctt cgcggtcgcg gtcgttggtg aggacgcggt gaaccagttt
     2041 gccgatgccg tcgcccacat agctttcgac cacttttgca ggcagcggtt tcataccgag
     2101 ttgctccaac atcgcttctg cggcggcggc aaggtcgggg acggaatcgc acagtgtgcc
     2161 gtccaaatcg aaggcgacgg cttggacgtg ttcgatggct gcattcataa tctgatttcc
     2221 tgtcgggttg aaagggttta ttttaacatc gtttgcacag ggcttctaat gtatagattg
     2281 ttaacaatcg caacaggttt tgcaggcggt gttttcggat ttcggggcgg aatcgggcaa
     2341 gggtttgaaa tcccggcgat gttccaatta aatttttaaa aaataaaagg atgcctatat
     2401 ctttatttaa tttgacattt atttttcgga cgcgcgcgct gctttttcag gcggagtccg
     2461 ggctgattcc gtgcggcttg aaaaataaaa tcatatatag attaatcaaa tcgggcttat
     2521 gccgactaca cgctattaca ttttgtcggg aaattggtta tagtaatgag cgaaatgcac
     2581 gcccctgaaa atctttttgt gaaaaggaag caaaatgtct gaagcccttg caaaagaaac
     2641 cctcaatccg tttgaaatcg cgcgcaaaca ggtcaaaacc gcctgcgacc ggctgaaaac
     2701 cgatccggcc gtttatgaaa tcctgaagag tccgacccgc gtgttggaag tcaatttccc
     2761 cgtcaaactc gacgacggca cggtcaaaac cttcacgggc taccgttcgc aacacaacaa
     2821 cgccgtcggc ccctataaag gcggcgtgcg cttccatccc ggtgtgaatc tggacgaagt
     2881 caaagccctg tcaatttgga tgaccatcaa atgctgcgtg gccggcattc cttacggcgg
     2941 cggcaaaggc ggcgttacct tggatccgcg cgattattcc gaggcggaac tggaacgcat
     3001 cgcccgcgct tattccgaag ccatttctcc gctcatcggc gaaaaaatcg atattcccgc
     3061 ccccgatgtg aataccaacg gcaaaattat gtcgtggatg gtggatgctt atgaaaacgt
     3121 ggtgaaacat tccgcgccgg gcgtatttac cggaaaaccg gttgagttcg gcggctcttt
     3181 ggcgcgtacc gaggctaccg gttacggcgt gaacctcgcc gccgtccaag ctttggaaaa
     3241 actgggcaag gacgtgaaag gcgcgactta cgccatccaa ggcttcggta atgtcggtta
     3301 ccacacaggt tattacgcgc atcaatccgg cgcgaaagtt gttgcggttt ctactgttga
     3361 cgttgccatc tacaatgaaa atggcttgga tatggaagcc ctgtttaaag agttccaaga
     3421 aaaaggcttc atcaccaacg aagccagtta cggcaaagaa attaccaacg ccgaactttt
     3481 ggctttggat gtggacgtac tcgccccttg cgcgttggaa aaccaactga cttccgaaaa
     3541 cgccggtaaa gtccgcgcga aaatcgtggt cgaaggcgcg aacggcccga ctacgcccga
     3601 agccgacgtt atcctgcgtc aaaacggcgt attggtcgtg cctgatattt tggcgaactg
     3661 cggcggcgtg gtggtatctt attttgaatg ggtacaaaat ctgcaaggct actactggga
     3721 gtttgacgaa gttcaggaaa aagaaaccgt cgtcctgcgc cgtgcgttcc gcgatatatg
     3781 gaacttggcg caagagtatg atgtcgattt gcgaaccgcg tcttacatga tgagtatccg
     3841 ccgtgttgaa aaagcgatga aactgcgcgg ttggtattga tgtttgaaca aaaaatgccg
     3901 tctgaagcct tcagacggca ttttgtgcgg gcgggattaa tggtgcgaat ggccgatttg
     3961 gaaggttaaa gtcgagtagt tcgcctgttt ttggcacacg ctttgatcgg ggaagtcggc
     4021 tttgtgttcg acattggctt tccagaagcc ttggcgcaag gggatgatgt ccacttcgcc
     4081 tttgtcgtct gtgctgtcgg agaaagcctg tgcttcggtt ttgtgcgttt tgctgcggtc
     4141 gctggtgtcg aagccgtcaa aggtggcggt aacggtggca ttgggcagcg gttcgccacg
     4201 gaacagaacg cggactttga agcgttcgcc tacgtgaatg ttggcgggat tgtccagcgg
     4261 gacgatttcc aagttttgtc cgaccggttt ggtgatgatg gcggtgtccg cgctttcgtg
     4321 tccgacgttg acgatgtttt tgccgaacat tcgggtttgt tcgcaatagc ttgcgtcagg
     4381 catttctttg atgcccgcct gtttccagcc tgctttgttt tttgaccaga aagtaggctg
     4441 atattcggcg atgacgaggt aactgccgtc cttaacggga cggttgcttc ggtactggta
     4501 gttgtatgtg ccgcgttgaa tcatgttttc cttgcctttt tcggtaacca gctgcatcgg
     4561 tttgctgaag atgtgcaggc ggtctttggc gatgggttcg agttcgggaa attcgccgta
     4621 gcccaagtcg gctttaaggt attcgccgcc gtgcgtgtgg gcggtttcga cccagacgcg
     4681 gtgggcgtgg gcggatgtgg caaatagggc ggaaacggcg agcagtgtca atgttttctt
     4741 catggtgcag cctttgtctc gtgtgggtta aagattttgt tatgttataa caaaataggt
     4801 atcgtgtgaa gacggatttg ctgtctgaaa actgtctttt tgtatagtgg attaacaaaa
     4861 accagtacgg cgttgc
//
LOCUS       DAXHPG010000149         4860 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXHPG010000149 DAXHPG010000000
VERSION     DAXHPG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.149"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(209..937)
                     /gene="rmpM"
                     /locus_tag="ACFM6F_001786"
     CDS             complement(209..937)
                     /gene="rmpM"
                     /locus_tag="ACFM6F_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC6165161.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEVSVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1301..2251
                     /locus_tag="ACFM6F_001787"
     CDS             1301..2251
                     /locus_tag="ACFM6F_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC6165162.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNTGSSIARAFAKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2364..3098)
                     /gene="fnr"
                     /locus_tag="ACFM6F_001788"
     CDS             complement(2364..3098)
                     /gene="fnr"
                     /locus_tag="ACFM6F_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC6165163.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGAYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3301..4722
                     /gene="hemN"
                     /locus_tag="ACFM6F_001789"
     CDS             3301..4722
                     /gene="hemN"
                     /locus_tag="ACFM6F_001789"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC6165164.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1239 a         1333 c         1175 g         1113 t
ORIGIN      
        1 cggcaatggg acacaaacct gaatctgaat ccgaacctgc accccgattc tgacgatagc
       61 cgtttcagaa aaccacccaa aaaaacaaac cccgcaaaag cgggggtttg tttcacatca
      121 tcgatgccga ccgacgtttc acgaatattg caacagtacc tggtacaaaa tccactaacc
      181 tcatgcatcg gcaagatatt gcctagcctt agtgttggtg atgattgtgt gccggcacaa
      241 cctgacgggt tacgatgctg cggattttca catccacacg gcggtcaggt tcgatacatg
      301 caatcagagc ctcacgtttt ttggctttag agactttcgc acccagtttg gcaacttcgg
      361 cttcacaaac ttgagtcatt tgcgcttgag attcgcccaa gccgacagca gaaattctag
      421 aaacaggtac gccgttgctg accaggttgt ttgccactac gtatgcgcgg cgttcggaca
      481 gggcctgatt gtatttgtca gaacccataa agtcggtatg gccttcgacg cggacagatt
      541 ggatattggt ttgacccagg cgttgcgcca atactttcag gttgtcttga gcttcggcgc
      601 gcaatgaatc cttatcgaaa ccgaacaggg ttttggcaga cagggaaatg gtttcatcaa
      661 catattgcgg agcctgctcc acaacgacga caggagcggg tgcgggttcg ggttctggct
      721 cgggttcggg ggcagcaacc gcatcgccgc attctacgcg accttggctt gctttatcaa
      781 agtaggcgtt tttccagcat tcgccatagt tgttgcgtac gatttcgttc gactggccgc
      841 ttacggtgta accctgaacg gacacctcgc ccgcaacagc agtgccggaa gcgagcaatg
      901 caacgaataa tgcgcttaat ttcagctgtt tggtcatttt attccctcat taaatttgta
      961 cagcagatac agaagcaact gctgaattgc caacatacag gccgctgtag aaacatggcg
     1021 gcaaatcgga tatttcagtc tccactataa agaagcaggg ggcggactgt caatactcaa
     1081 cgcctgaaaa caaccgtaaa acagcctgtt tcctgatagt ctatcatgcc gaaaaatcaa
     1141 ttccgtgtca ttactttagg gggatttttt ccatatcgca cgattgccgt tgcacaaaaa
     1201 caacaaacac aacagcaaag ccccataccg taccggcaag aaaatatgat aaattataaa
     1261 caatgttcag ccacccgaca cagacccaca ccgacccgcc atgaaattac aacaattgaa
     1321 atacgcctta gaagtttacc agcacaacct gaacgtttcc gaagcggccg aagccttgtt
     1381 tacttcgcaa cccggcatct ccaaacaaat caaattgctg gaagaagaaa tcggcattca
     1441 gatttttatc cgcagcggca agcgcgtggt ttcggtctcg cagccgggca aggtggtttt
     1501 ggatattgcg gaacgtattt tacgcgatgt acagaacatt aaaaatatcg gcagcgagtt
     1561 taccggacag gacagcggtt cgctgacggt tgccacgacg catactcagg cacgctatgc
     1621 cctacccttg attgttgccg attttgtgaa acgctatccg aaagtcaacc tgaccatcaa
     1681 acaggggagc cctgccgcca tcgcacgtat ggttacctca ggcgaggcgg acttggcgat
     1741 tgttacggaa cggatagacg accatcccga actgggaaaa cttccctgct atgactggac
     1801 tcatgcggtt atcgtaccga acgaccaccc cttgctcgaa tgcagaaacc ccctccgcat
     1861 tgaagatttg gcaagatttc cactaattac ctatgaattt gcattcaaca cgggcagcag
     1921 cattgcacgg gcattcgcca aagctcgttt ggaacaaccc gatgtcgcat tggctgcggc
     1981 agatacggac gtattgaaga cttatgtgcg cttgggtttg ggcgtgggac tgatggcgaa
     2041 aatggcgtac aacccggata cggacggcga tttgcagctt gtggatgcgg cacacctgtt
     2101 cgagccgtcg ccgacgtgga ttgctttgcg cagcgatact tatttgcgcg gatatgccta
     2161 cgactttatc caagcgtttg cgccgcacct gacacgcgag aaggtggata ggattctgta
     2221 cacgcccatc agcgaggatt tttcgattta ggcggctgcc ggttttcaga cggcactttg
     2281 cggcagatac aacaaacagg acagatgttt tcgtctgccc tgtgtttatt gggaatgccg
     2341 tctgaaatgt tcgtacgggt taatcaaatg gcgtgcgaac aaccggatac catcttcttc
     2401 agcacctgca gattgaggat cttgatgtgt ttgtgttcga cggaaatcaa tccttcctga
     2461 tggaacttgg ataatgtgcg gctgacggtt tcaagtttca gcccgagata actgccgatt
     2521 tcttcgcggg acattcttaa gatgaagtcg ttggcagcaa aacctcggga ataaaggcgt
     2581 tgggaaaggt tcagcaggaa ggcggcaatc cgctcttcgg cgcgcatatt gcccaacagc
     2641 agcataacac cttggtcgcg cacgatttca cggctcatca tgcggaagaa gtgcgtacgc
     2701 aggctgggga tgttttgccc cagttcttcg atgtgggtaa acggcagttc gcacacttcg
     2761 ctgtcttcca aggcgaccgc gtcgcaactg tgcacatggg aacagatgcc gtccatgccg
     2821 atgagttcgc ccgacataaa gaaacccgtt acctgatcgc ggccgtcctg actggcgacg
     2881 gttgttttga agaagcccga acggatggca aagagcgagg taaaggcttc gccgacacag
     2941 aacaggtatg cgcccttttt caggcggcgg ctttgacgga tgacggcatc gagttggctg
     3001 agctcgttgg gcagcagccc gacaggcagg cagagttccc gcaaagaaca ggaagaacac
     3061 agcgttttca tctgatgtgt agtattatgc gaagccatac cgtacctttt tgtgcgcttt
     3121 gccccatcat gattatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgtcc
     3181 ttatttaaat ttaatccact atatgtgctt attgacacat atcaagacag gtttatcata
     3241 ctgtggcatt ctaccaaact atccaaaatt tgactaacat cataaaaccg cacacacacc
     3301 atgaaaatca ttcagataca gaacaatcac aatgtaaacg atgaccgccc cgagtttgac
     3361 cgcgcgctga ttgccagcct gcccgccagc ggcccgcgct acacttccta ccctaccgcc
     3421 gaccgtttcc atgacggttt ccgcgaaggc gaatatatca aagctttaca tttgcgcggt
     3481 atgggcgcgt taaacaaacc gctttccctt tacattcaca ttccgttctg caacaccatc
     3541 tgctactact gcggctgcaa caaaatcatc accaaagaca aaagccgcgc cgatgcctac
     3601 atcgaatatc ttgaaaaaga aatggaactg ctcgctccac atctgaacgg acggcaccag
     3661 cttgcccaac tgcacttcgg cggcggcacg ccgacctttt tgagcgacga acagatcgaa
     3721 cgtgtcttcc gcatgatacg caaacatttc gagttaatcc ccaccggcga atactccatc
     3781 gaaatcgacc cgcgcaaagt cagccgcgac accgtcctca tgctcggcag actcggcttc
     3841 aaccgcatga gcatcggcat tcaggatttc gaccccaaag tgcaggcggc ggtcaaccgc
     3901 atccaaagtt acgaagaaac caaagaagtc atcgatgcgg cgcgcgaagc ggggttcaaa
     3961 tccgtcagcg tcgatttgat ttacggcctg ccgcaccaga cttcggaaag catcaaaacc
     4021 accatcgata ccgttttgtc gctcgatccc gaccgcctcg ccctttatca ctacgcccac
     4081 ctgccgcacg tgttcaaacc gcaacgccgc atcgataccg ccgccgttcc cgacagcgaa
     4141 gagaagctcg atatgctgca atactgcgtc caaaccctaa ccgaacgcgg ttacgtcttc
     4201 atcggcatgg accatttcgc caaacctgac gacgaactct ccatcgccct caaagaaggc
     4261 ttcctccagc gcaacttcca aggctattcg acctacgcgg attgcgattt ggtcgccatc
     4321 ggcgtgtcgt ccatcggcaa aatcggcagc acctattccc aaaacgaacg cgacatcgat
     4381 gcctactatg ccgccatcga cgaaggcaga ctgcccatca tgcgcggtta tcagctcaat
     4441 caggacgaca tcctgcgccg caacatcatt caggatttga tgtgccgctt cgcgctcgac
     4501 tatcggattt acgaaagtat gttcggcatc ccgttcgacc gctacttcaa agacgaactg
     4561 gcggatttgg aaaaactcgc cggtttggga ttggtgcgcc tgaacagcca cgggctgacc
     4621 gttaccccga aaggacgctt cctcatccgc aacatcgcca tggtcttcga ttaccacctg
     4681 cgccacaaag aaaccaaggc gaaatattcg caaacggtgt gattgcgtct aacgtacaaa
     4741 tgccgtctga aaggcttttt cagacggcat tttgctgccg gcaggataag tgttttcaag
     4801 aacaggcggc ggcatatcat aacgttccgc acctttgtgt ccgaccgttc cgaaaccaag
//
LOCUS       DAXHPG010000150         4810 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXHPG010000150 DAXHPG010000000
VERSION     DAXHPG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.150"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..1064
                     /gene="mafB"
                     /locus_tag="ACFM6F_001790"
     CDS             <1..1064
                     /gene="mafB"
                     /locus_tag="ACFM6F_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6165165.1"
                     /translation="AAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGK
                     FAVIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEALVNVLPFAKVKNLTKAAKPGK
                     AAVSGDFSKSYTCSFHGSTLVKTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTTRY
                     GNPYQETVYIEVSDGIGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTV
                     QNITLKQQPLKAYNLTVADWHTYFVKGDKAETEGVWVHNSCPPYKRPNNATTKAQRES
                     VQGKPCVECGKLAEKMIADHKKPLVVEYYETGTIDKSKMRAIESVQPQCPTCSAKQGG
                     RLSQYSKEQKRKLSNGNKK"
     gene            1048..1401
                     /locus_tag="ACFM6F_001791"
     CDS             1048..1401
                     /locus_tag="ACFM6F_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165166.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQANLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            1391..1714
                     /locus_tag="ACFM6F_001792"
     CDS             1391..1714
                     /locus_tag="ACFM6F_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC6165167.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKSMIIEFFIEGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFDIAQKNDCNYEGF
                     GSYGN"
     gene            1949..2218
                     /locus_tag="ACFM6F_001793"
     CDS             1949..2218
                     /locus_tag="ACFM6F_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165168.1"
                     /translation="MSVKELFKVILDKNKDFPIRTIHRTPIGILPKPVALSIVQYEDD
                     QGFYLFYLDETGQEQTDTYHDTLDSAFEQVEFEFGISKEEWMQSP"
     gene            2200..2352
                     /locus_tag="ACFM6F_001794"
     CDS             2200..2352
                     /locus_tag="ACFM6F_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165169.1"
                     /translation="MDAKPLGGNIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            2339..2884
                     /locus_tag="ACFM6F_001795"
     CDS             2339..2884
                     /locus_tag="ACFM6F_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC6165170.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVGNLSDMNRKPFVW"
     gene            3018..3647
                     /locus_tag="ACFM6F_001796"
     CDS             3018..3647
                     /locus_tag="ACFM6F_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC6165171.1"
                     /translation="MVKTADGYKAIAHIRVGESVLSKDEASGKTGYKPVTAQYGNPYQ
                     ETVYIEISDGIGNSQTLVSNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGDKAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQIS
                     AESKIAELTNKIIPGMSKNERLKIERTIRNITKNANRKAKGEEHGRRGR"
     gene            3628..4083
                     /locus_tag="ACFM6F_001797"
     CDS             3628..4083
                     /locus_tag="ACFM6F_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165172.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITEYKNILETFMYEPPDEF
                     RTEYYESPDSNLLISVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            4090..4146
                     /locus_tag="ACFM6F_001798"
                     /pseudo
     CDS             4090..4146
                     /locus_tag="ACFM6F_001798"
                     /inference="COORDINATES: protein motif:HMM:NF026864.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin C-terminal domain-containing protein"
     gene            4161..4538
                     /locus_tag="ACFM6F_001799"
     CDS             4161..4538
                     /locus_tag="ACFM6F_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC6165173.1"
                     /translation="MCELKNFRRNITCFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRYVVIGIGTIIDFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVRFDYYNKK"
BASE COUNT         1607 a          929 c         1000 g         1274 t
ORIGIN      
        1 tcgccgccgg agcactcaac ccctttatca gcgcgggcga agccttaggc ataggcgaca
       61 tactgtacgg aacgcgctat gccatagaca aagccgcaat gcgcaacatc gcccccttac
      121 ccgccgaggg caaattcgcc gtcatcggcg gcttgggcag cgtggcgggc tttgaaaaga
      181 atacgcgcga agccgttgac cggtggatac aggaaaaccc caatgccgcc gaaaccgtcg
      241 aagccctggt caacgtcctg ccgtttgcca aagtcaaaaa cctgacaaag gcggcaaaac
      301 cggggaaggc tgcggttagt ggggattttt ctaaatccta cacctgctcc ttccacggca
      361 gcaccttggt caaaacggca gacggctaca aagccattgc ccatattcaa gccggagacc
      421 gcgtcctttc caaggacgag gcaagcggag aaacgggata caaacccgtt accacccgat
      481 acggtaatcc gtaccaggaa accgtttaca ttgaagtttc agacggcatc ggcaacagcc
      541 aaaccctgat ttccaaccgc atccatccgt tttacagtca aggaaaatgg atacaggcag
      601 gtcgtctgaa aaaaggcgac accctgcttt ccgaaagcgg cgcaaaacag acggttcaaa
      661 acattaccct caaacagcag ccgctcaaag cctacaacct gaccgttgcc gactggcata
      721 cttacttcgt caagggagat aaggcggaaa cggaaggggt gtgggttcat aattcttgtc
      781 cgccatataa gagaccaaat aacgcaacga caaaagcaca acgagaaagt gtacaaggaa
      841 aaccctgcgt tgaatgcggt aaattagccg aaaaaatgat tgccgaccat aaaaaaccat
      901 tagttgtcga gtattatgaa acagggacaa ttgacaaatc taagatgaga gcaattgaat
      961 cagtacaacc gcaatgtccg acttgttctg caaaacaagg agggagatta agccaatatt
     1021 caaaagaaca gaaaaggaaa ctgagtaatg ggaataaaaa atgaacaaat aataatttgt
     1081 aaaaaatata atacagaaat atatcctgtt tccgatgttt caaaaatcgg tgttgctgaa
     1141 aatgtcaaac aggcaaacct gtatccgatt aacgggttaa ggcaccgtcc caaaggtgat
     1201 actaacggtt ggtatatctg ggccggtgaa aacttctcct atgataaaaa ttttttcctg
     1261 cccttgcata ctttccattt gcaaatatgg cgacccgaaa tcatcccctt tttgacactt
     1321 ccgcccggtt accgtttttt aattggggaa aacggttatg aagacgtatg gtttgatgag
     1381 ttgctattaa atgataattg acgaaaaatc tacttcagat gtactgaaac aatatcaaga
     1441 actagggttt gatttgacaa aatcgatgat aatagagttt tttatagagg gttctcaaaa
     1501 aaatctgcaa tcaatagaaa gccaactcat ttcttatcgg caagattttc aaatcagtat
     1561 tgaacaagat gaattcggag aaatgtggac ttgctattgt tccgtcaata tcgtcccttc
     1621 ccttgaaaac attttagcta ttgaaactac gttatttgat attgcacaaa aaaatgattg
     1681 taattatgaa ggttttggtt cgtatgggaa ctaacaagaa gtgtaggttg ggttgaaaaa
     1741 cacaacaatc aaaaggtcgt ctgaaaccat gtttaatttt cagatggctt ttccttcatt
     1801 taaaacagga tattgagatc taagttcttt aaaaatccta cacctgctcc ttccacggca
     1861 gcaccttggt caaaacggca gacggctaca aagccaatgg acgtgtaata gaaaatagtg
     1921 cagtaacaat tattcggatt aaaaaattat gagtgtaaaa gaattattca aagttatctt
     1981 agataaaaat aaagattttc ctattagaac gattcatcgc acgccaatag ggatacttcc
     2041 caaacctgtt gctttaagta ttgttcaata tgaagatgac caaggatttt atttgtttta
     2101 tttggatgag accggtcagg aacaaacgga tacttatcat gacacattag attcggcatt
     2161 tgaacaggtg gagtttgaat ttggaattag caaagaagaa tggatgcaaa gcccttaggt
     2221 ggcaatatca agatgccgct ttatttttca gacggcattt ccgtcaataa aaaagtcggt
     2281 attggagccc aaaacagcct acgtcttgcc gggataggga tgaaatcttt aagaaaaaat
     2341 gattatagtt gaacatttag aacattattt gggtgaaatc gaaagcggta taaaatgctt
     2401 ggatcgacgt tatcatctca gcgtttccgt cttcccatca caaccatata aaggggtaac
     2461 tactttttct actttaggct tgaatcgata tgacttgaac tataagagta ggtttgaatt
     2521 aatttttacc tgttctgaag aatggaataa agaaaatatt gcagcttttc tatcaggagt
     2581 agccgaatat ttgattgaca atagacaacc tattttaaga ggtgaaatta ttcaattacc
     2641 tagggtaatt atcgaaggta gcaaaatgga cgcactgtat gtttccgccc cattttattt
     2701 cgacgatgat tttcaggtct gttatggcga acactacaat atcgttttcc ctttgcttgt
     2761 cccgttgtat aaacaggaag cagaattggt ggaaaagaaa ggttggaatg cttttgagca
     2821 gttcttgctg gataatgaag ttggcaacct ttcggatatg aatcggaaac cgtttgtttg
     2881 gtaagggctt tcttaaattt gattaaaggt cgtctgaaac cgtgtttaac tttcagacaa
     2941 cctttccttc atttgaaaca ggatattgaa aactaagttt ttcaacattc tacacctgct
     3001 ccttccacgg cagcaccttg gtcaaaacgg cagacggcta caaagctatt gcccatatcc
     3061 gagtcggcga gagcgttctt tccaaggacg aggcaagcgg gaaaacagga tacaaacccg
     3121 ttaccgccca atacggtaat ccgtatcaag aaaccgttta cattgaaatt tcagacggca
     3181 tcggtaacag ccaaaccctt gtttccaata aaatccaccc gttttacagt caaggaaaat
     3241 ggatacaggc aggtcgtctg aaaaaaggcg acaccctgct ttccgaaagc ggtgcaaaac
     3301 agacagttca aaacattacc ctcaaacagc agccgctcaa agcctacaat ctgaccgttg
     3361 ccgactggca tacttacttt gtcaaaggcg ataaggcgga aacggaagga gtttgggttc
     3421 ataattcttg tccgcctaaa agaacaggaa gctccaagaa tgaaaaacat ggagatggcg
     3481 gtcgaagtca aatatcagcg gaatcaaaaa ttgctgaatt aacaaataaa attattcccg
     3541 gaatgtccaa aaatgaacgc ttaaagattg agcgaacaat cagaaatatt acaaaaaatg
     3601 ccaatcgaaa agcaaaagga gaagagcatg gtcgacgcgg tcgttaaaac tcccgaattt
     3661 ttacctttta cttctgtagg aatattccgt tttggtgcgg atataacaga gtacaaaaac
     3721 atactggaaa cttttatgta tgaacctcct gatgagttta gaacagaata ttatgaatct
     3781 cccgactcaa atttactcat ttccgtcaaa aaaaacaaaa ttatttcgat attttgttat
     3841 caggagctat attttatggg cgttaatata ataggtctga attttgaaga ttttaaacag
     3901 cttttccatc accctaaatc atacggtgtt gataaatatt atttatcaaa tgaatcttat
     3961 ccgacatatg tctatgagtt tgatgaaata ggagtacaag catgggaagc aaaagggaaa
     4021 attgttacta tcattgccgg gggcaaagac aattattcaa cagaacctta ctacgatgaa
     4081 taacattaca gaaaagaaac aagaaacggt acatttgata aaaacctaaa tcgtattgga
     4141 gattaattat tatgtgtatt atgtgtgagt taaaaaactt cagaagaaat attacttgtt
     4201 ttgagggata tgacgaaaat tcatttattg gcaaatggta tgatgacggg gtgtgggatg
     4261 atgaagaata ttggaaattg gagaatgatt taatcgaggt tagaaaaaaa tatccttatc
     4321 cgatggatat accaagatat gttgtcattg gaatcggtac cattattgat ttcttaatgg
     4381 ttccaaattg gaaacttttt gaaattaaag cttccccttg gctacctgat agcgtaggaa
     4441 ttaatgaacg ttatgaaagg cttaaaacaa tgctccgtta tatttttacc gagaaagaca
     4501 tagtcaacgt gcgatttgat tattacaaca aaaaatagca aaccccggca atcaaaatgc
     4561 cgtctgaagc gatatttgct tttcagacgg cattttgatg aatgaaacag gatattgaga
     4621 actaagttct tcaaaaatcc tgcctccgtc ccttatacgt cggtgcttta gtcaaaacgg
     4681 cagacggctg aaaagcaaac actgtcatca accatttgaa gaccggatgg aataggaaag
     4741 aaaatcggta aaatctccac gattagacgg taaaatacct cggtgtcgct gaaaccctga
     4801 tcgtcgtgtt
//
LOCUS       DAXHPG010000151         4794 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXHPG010000151 DAXHPG010000000
VERSION     DAXHPG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.151"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..401)
                     /locus_tag="ACFM6F_001800"
     CDS             complement(<1..401)
                     /locus_tag="ACFM6F_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HFC6165174.1"
                     /translation="MLCPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASLQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQA"
     gene            complement(437..550)
                     /gene="rrf"
                     /locus_tag="ACFM6F_001801"
     rRNA            complement(437..550)
                     /gene="rrf"
                     /locus_tag="ACFM6F_001801"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(647..3538)
                     /locus_tag="ACFM6F_001802"
     rRNA            complement(647..3538)
                     /locus_tag="ACFM6F_001802"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3947..4022)
                     /locus_tag="ACFM6F_001803"
     tRNA            complement(3947..4022)
                     /locus_tag="ACFM6F_001803"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3987..3989),aa:Ala,seq:tgc)
     gene            complement(4028..4104)
                     /locus_tag="ACFM6F_001804"
     tRNA            complement(4028..4104)
                     /locus_tag="ACFM6F_001804"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4068..4070),aa:Ile,seq:gat)
     gene            complement(4206..>4794)
                     /locus_tag="ACFM6F_001805"
     rRNA            complement(4206..>4794)
                     /locus_tag="ACFM6F_001805"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1044 a         1384 c         1042 g         1324 t
ORIGIN      
        1 gcctgatcct tcagcttcgc cagcaccgca gggccgatgc cctttacctt ggtcaaatcg
       61 tctacagact tgaacgcacc gttttgcgca cggtattccg caatggcctt cgccttcgcc
      121 gggcctatgc ctggcagcgc ctccaactcc tgcagcgaag ccgcattgat gtttaccgcc
      181 gcaagggaga aggcgcagga gaacagcata cagaacagca cgaacatttt cttcatggtt
      241 tttcctttaa gggttgcaaa caataaaccg catcttgcga cgataaaacg agtcattcta
      301 aaatgaatat cccaaagttt caagccgttc ctccgcaaac ccgaccggac gccgtacgga
      361 tgccgtcccg ccatccccga cattttttcc gggcaaagca aaaacccccg aataatcggg
      421 ggttttctga atgggtgttt ggcagtgacc tactttcgca tggaagaacc acactatcat
      481 cggcgctgag tcgtttcacg gtcctgttcg ggatgggaag gcgtgggacc aactcgctat
      541 ggccgccaaa cttaaactgt tacaaatcgg taaagcctta atcaatatat tcggtaatga
      601 ctgaatcagt cagtaagctt ttatctcttg aagttcttca aatgatagag tcaagcctca
      661 cgagcaatta gtatgggtta gcttcacgcg ttaccgcgct tccacacccc acctatcaac
      721 gtcctggtct cgaacgactc tttagtgcgg ttaaaccgca agggaagtct catcttcagg
      781 cgagtttcgc gcttagatgc tttcagcgct tatctcttcc gaacttagct acccggctat
      841 gcaactggcg ttacaaccgg tacaccagag gttcgtccac tccggtcctc tcgtactagg
      901 agcagccccc gtcaaacttc caacgcccac tgcagatagg gaccaaactg tctcacgacg
      961 ttttaaaccc agctcacgta ccactttaaa tggcgaacag ccataccctt gggaccgact
     1021 acagccccag gatgtgatga gccgacatcg aggtgccaaa ctccgccgtc gatatgaact
     1081 cttgggcgga atcagcctgt tatccccgga gtacctttta tccgttgagc gatggccctt
     1141 ccatacagaa ccaccggatc actatgtcct gctttcgcac ctgctcgact tgtcggtctc
     1201 gcagttaagc taccttttgc cattgcacta tcagtccgat ttccgaccgg acctaggtaa
     1261 ccttcgaact cctccgttac gctttgggag gagaccgccc cagtcaaact gcctaccatg
     1321 cacggtcccc gacccggatg acgggtctag gttagaacct caaagacacc agggtggtat
     1381 ttcaaggacg gctccacaga gactggcgtc tctgcttcaa agcctcccac ctatcctaca
     1441 caagtgactt caaagtccaa tgcaaagcta cagtaaaggt tcacggggtc tttccgtcta
     1501 gcagcgggta gattgcatct tcacaaccac ttcaacttcg ctgagtctca ggaggagaca
     1561 gtgtggccat cgttacgcca ttcgtgcggg tcggaactta cccgacaagg aatttcgcta
     1621 ccttaggacc gttatagtta cggccgccgt ttactggggc ttcgatccga tgctctcaca
     1681 tcttcaatta accttccagc accgggcagg cgtcacaccc tatacgtcca ctttcgtgtt
     1741 agcagagtgc tgtgttttta ataaacagtc gcagccacct attctctgcg accctccggg
     1801 gcttacggag caagtcctta accttagagg gcataccttc tcccgaagtt acggtatcaa
     1861 tttgccgagt tccttctcct gagttctctc aagcgcctta gaattctcat cctgcccacc
     1921 tgtgtcggtt tgcggtacgg ttcgattcaa actgaagctt agtggctttt cctggaagcg
     1981 tggtatcggt tacttcgtgt ccgtagacac tcgtcgtcac ttctcggtgt taagaagacc
     2041 cggatttgcc taagtcttcc acctaccggc ttaaacaagc tattccaaca gcttgccaac
     2101 ctaaccttct ccgtccccac atcgcatttg aatcaagtac aggaatatta acctgtttcc
     2161 catcgactac gcatttctgc ctcgccttag gggccgactc accctacgcc gatgaacgtt
     2221 gcgcaggaaa ccttgggctt tcggcgagcg ggcttttcac ccgctttatc gctactcatg
     2281 tcaacattcg cacttctgat acctccagca cactttacaa tgcaccttca tcggcctaca
     2341 gaacgctccc ctaccatgcc ggtaaaccgg catccgcagc ttcggttata gatttgagcc
     2401 ccgttacatc ttccgcgcag gacgactcga ccagtgagct attacgcttt ctttaaatga
     2461 tggctgcttc taagccaaca tcctggctgt ctgggccttc ccacttcgtt taccacttaa
     2521 tctatcattg gggaccttag ctggcggtct gggttgtttc cctcttgaca acggacgtta
     2581 gcacccgctg tctgtctccc gaggaaccac ttgatggtat tctgagtttg ccatgggttg
     2641 gtaagttgca ataaccccct agccataaca gtgctttacc cccatcagtg tcttgctcga
     2701 ggcactacct aaatagtttt cggggagaac cagctatctc cgagtttgtt tagcctttca
     2761 cccctatcca cagctcatcc ccgcattttg caacatgcgt gggttcggtc ctccagtacc
     2821 tgttacggca ccttcaacct ggccatggat agatcactcg gtttcgggtc tacacccagc
     2881 aactcatcgc cctattaaga ctcggtttcc ctacgcctcc cctattcggt taagctcgct
     2941 actgaatgta agtcgttgac ccattataca aaaggtacgc agtcacacct ctagggtgct
     3001 cccactgttt gtatgcatca ggtttcaggt tctgtttcac tcccctcccg gggttctttt
     3061 cgcctttccc tcacggtact ggttcactat cggtcgatga tgagtattta gccttggagg
     3121 atggtccccc catattcaga caggatttca cgtgtcccgc cctacttttc gtacgcttag
     3181 taccgctgtt gagatttcga atacgggact gtcacccact atggtcaagc ttcccagctt
     3241 gttcttctat ctcgacagtt attgcgtaca ggctcctccg cgttcgctcg ccactacttg
     3301 cggaatctcg gttgatttct tttcctccgg gtacttagat ggttcagttc tccgggttcg
     3361 cttctctaag tctatgtatt caacttagga tactgcacag aatgcagtgg gtttccccat
     3421 tcggacatcg cgggatcatt gctttattgc cagctccccc gcgcttttcg caggcttaca
     3481 cgtccttcgt cgcctatcat cgccaaggca tccacctgat gcacttattc acttgactct
     3541 atcatttcaa gaacttcttt gacttcgttt gcctacctgt tgactaagta aaagcaaact
     3601 tgaaattcct actttgataa agcttactgc tttgttgtgt cttaatcctg ccttttgtgt
     3661 ttcaggatta agtcgataca atcatcaccc aaatactgtg tttgttttct tttctcttgt
     3721 gagcgatttt ttatcctttg caaagaataa aaaatcaaaa caaacgcttt gtctttgttt
     3781 gttgatttcg gctttccaat ttgttaaaga tcgatgcgtt cgatattgct atctactgtg
     3841 caaatcaaaa cgagctgatt attatatcag cattttgttc ttggtcaagt gtgatgtcgc
     3901 cctgaatgga ttctgttcca ttcttccgtt ttgatttgta cagtattggt ggaggcaaac
     3961 gggatcgaac cgatgacccc ctgcttgcaa agcaggtgct ctaccaactg agctatgccc
     4021 ccgttattgg tgggtctggg aggacttgaa cctccgaccc cacgcttatc aagcgtgtgc
     4081 tctaaccagc tgagctacaa acccggattc tcttcttaag cgaatcttgc cttcactcaa
     4141 gcttcttccg catctttttc agtttaccga taagtgtgaa tgcctaaagc ctcttctttc
     4201 tctagaaagg aggtgatcca gccgcaggtt cccctacggc taccttgtta cgacttcacc
     4261 ccagtcatga agcataccgt ggtaagcgga ctccttgcgg ttatcctacc tacttctggt
     4321 atcccccact cccatggtgt gacgggcggt gtgtacaaga cccgggaacg tattcaccgc
     4381 agtatgctga cctgcgatta ctagcgattc cgacttcatg cactcgagtt gcagagtgca
     4441 atccggacta cgatcggttt tgtgagattg gctccacctc gcggcttggc taccctctgt
     4501 accgaccatt gtatgacgtg tgaagccctg gtcataaggg ccatgaggac ttgacgtcat
     4561 ccccaccttc ctccggcttg tcaccggcag tctcattaga gtgcccaact gaatgatggc
     4621 aactaatgac aagggttgcg ctcgttgcgg gacttaaccc aacatctcac gacacgagct
     4681 gacgacagcc atgcagcacc tgtgttacgg ctcccgaagg cactcctccg tctctggagg
     4741 attccgtaca tgtcaagacc aggtaaggtt cttcgcgttg catcgaatta atcc
//
LOCUS       DAXHPG010000152         4749 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXHPG010000152 DAXHPG010000000
VERSION     DAXHPG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.152"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..369
                     /locus_tag="ACFM6F_001806"
     CDS             <1..369
                     /locus_tag="ACFM6F_001806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165175.1"
                     /translation="GYDLSFGIRNIIIFFVKGIYTEDDSKPAGKKRPLLNCTPKVGHL
                     TDFRGCSFLWQNIQMNSDLPSFNTIWQGTADHLSISDSLVRRWVTKYRLHGESGIKRR
                     KHTTKYSVEYKLEAIRLVTG"
     gene            373..486
                     /locus_tag="ACFM6F_001807"
     CDS             373..486
                     /locus_tag="ACFM6F_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6165176.1"
                     /translation="MSQKAAADQLNLPDCSILLQWLRLYHLNGINGLKPKP"
     gene            806..1063
                     /locus_tag="ACFM6F_001808"
                     /pseudo
     CDS             806..1063
                     /locus_tag="ACFM6F_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1116..1298)
                     /locus_tag="ACFM6F_001809"
     CDS             complement(1116..1298)
                     /locus_tag="ACFM6F_001809"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6165177.1"
                     /translation="MKYTIFFSTSHHSNQTKATHRHTHVGSTAGQTTIRSGGDTTLKG
                     AQLIGIGIQADPQLQP"
     gene            complement(1449..2399)
                     /locus_tag="ACFM6F_001810"
     CDS             complement(1449..2399)
                     /locus_tag="ACFM6F_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC6165178.1"
                     /translation="MYLGIDVSKLTIDCCLIVDGQNYQKKFQNNKGGFEQLINWLQSH
                     KVNDKLHCVCEATGTYYEALAEYLYSRYTITVENPRKIKGYAIAELQRSKTDTQDAKL
                     IAQYCQDRKHKLKAWKPPTKEQKQLQEIARYLDYLKQQRATEKAKQHEAPDYIKSHIQ
                     TTISNLTAQIQIVKKQLLQFYKDNPSYNNLRKRLKTITGIGEQATAVLLSTYKRHEFK
                     NARQFTAYLGLDPRKFQSGTSVNGKSRISKIGSSEIRKSLYMPALVAYRCNAFPEFVG
                     RLKNKGKHIKLILIAIMRKLAVIAFTILQNGQDFQVERYK"
     gene            complement(3025..4110)
                     /locus_tag="ACFM6F_001811"
     CDS             complement(3025..4110)
                     /locus_tag="ACFM6F_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC6165179.1"
                     /translation="MIYLFTGNMGTGKTSRVVSMILNNEDGLFKMKLEDGTEVDRPLY
                     FCHIDGLDKRQFKAHELTEEQIMSAPLRDVIPEGAVLIVDEAHYTYPVRAAGRPVPPY
                     IQELTELRHHGHTVILMTQHPSQLDIFVRNLVSKHVHLERKAIGMKQYYWYKCVTSLD
                     NPAGVSGVEVASWKPPKEAFKYYKSASQHQKFKKKVPWAVWALIAIVGFVGWKSYGIF
                     KVYSKATDSRIEQEAQKESVVQTMTEQPASSEEMPLKNSDNLKPEDFVPTLPEKPESK
                     PIYNTVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNPYK
                     DEQQRTEQVEQSAKADKPQVLVMGGKP"
     gene            complement(4120..4410)
                     /locus_tag="ACFM6F_001812"
     CDS             complement(4120..4410)
                     /locus_tag="ACFM6F_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC6165180.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLIALEKFK
                     DYTSNAINSMPSDILNLLLISGFGQGLGYLFGAFSFFIGMHAFKKLTFVFPG"
     gene            complement(4411..>4749)
                     /locus_tag="ACFM6F_001813"
     CDS             complement(4411..>4749)
                     /locus_tag="ACFM6F_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6165181.1"
                     /translation="RHRKERKEGEDGGLLCDYFPEILACQEMGKPSDGMFHDISIPQV
                     IDDKTWSSHNFLPSNGVCPQPKTFHVFGRQYQASYEPLCVFAEKIRFAVLLAFIIMSA
                     FVVFGSLKGK"
BASE COUNT         1182 a         1038 c         1037 g         1492 t
ORIGIN      
        1 ggatacgacc ttagttttgg tatccgtaat atcatcattt ttttcgtcaa gggaatatac
       61 acggaggatg attcaaaacc ggcaggcaaa aaaagaccgc ttctgaactg caccccaaaa
      121 gttggacacc ttaccgactt tagggggtgc agttttttat ggcaaaatat tcagatgaat
      181 tccgacttgc cgtcgttcaa tactatttgg cagggaacag cagaccatct ttctatttcc
      241 gattcattgg tacgcagatg ggtgacaaaa tacagattac acggagagag tggcatcaaa
      301 cgtagaaagc atacgacaaa atattcggtc gaatacaaac ttgaggcaat ccgcctggtg
      361 acggggtagg gaatgtccca aaaagctgcc gcagaccaac tgaatttgcc cgactgctcc
      421 atcttgctgc aatggttgcg cctctaccat ttgaatggta ttaacggttt aaagcccaaa
      481 ccctaaaccc aaaggaagaa agcccgtgaa aaaacagcat ccgccggaaa cgaaaaagcc
      541 gactatctga aaaccaagga agaactgctt gcggaattgg cttgccttaa agcggaaatg
      601 gctgccctaa aaaagctcga tgccttaatc tatgggaaag aagtgcggta gaaagaacgc
      661 aactcgtcgc agggttaagg caatgccatc cgttgaactg ctgttggtga ttgtcggact
      721 gccacgcggc accttctatt accaattggt tgtccaatcg gcagaagaca aatatgccga
      781 tttgaaacgg catatccatg atatttatca aaagcagttg aaagacaacg gtctggttca
      841 gagtatgtcc cgcaagggaa actgcttgga caatgcggca atggaaagtt tcttcggaac
      901 gttgaaatcg gaatgtttcc atacgtgcaa atatgattcc gttaccgaat tggaagctgt
      961 actgcacgaa tatatccgtt actacaacaa cgatagaatc aagttgaaat taaaaggact
     1021 gagccctgtt cagtacagaa ttcagtccct gaaagccgct tgattaaact gtccaacttt
     1081 tgggggtcag ttaatatcgg ttccaccaat agtcgtcagg gttgtagttg ggggtctgcc
     1141 tgtatgccta tgccgataag ctgagcacct ttgagggtgg tatccccgcc gcttcggatg
     1201 gtggtttgtc cggccgtact gccgacatgg gtgtggcggt gggttgcttt tgtctgattg
     1261 ctgtggtggc ttgttgaaaa aaagatagtg tatttcacgg tcaaataact ttacatttct
     1321 tgaagttgca agaatgcgca ccccgacttt ttcgggatgt ttgtttcgtt ttgaatggat
     1381 ttgatgtttt aatttatact ttattttcaa taaattgaaa atcaccggcg aaagcccagt
     1441 ttaatttttt atttatatct ttccacttgg aaatcttggc cgttttgcaa aatcgtaaac
     1501 gctattaccg ccagtttccg catgatggca attaatatca attttatatg cttcccttta
     1561 tttttcagac gccctacaaa ttcagggaag gcattacaac gatatgcaac aagtgcaggc
     1621 atataaaggc ttttccttat ttccgaactt cctatttttg atattctgct ttttccgttc
     1681 acgcttgttc ctgattgaaa ttttctaggg tctaggccta gataagccgt gaactgtctt
     1741 gcatttttaa attcatgtct tttataggtt gatagcaata ctgctgtcgc ttgctcgcct
     1801 atgcctgtta ttgttttcag ccttttgcgt agattgttat aacttggatt gtctttgtag
     1861 aactggagta attgcttttt gactatctgt atttgtgctg tcaggtttga aatagttgtt
     1921 tgaatatggg atttgatata gtcgggtgct tcgtgttgtt tagctttttc tgttgcgcgt
     1981 tgctgtttca gatagtctaa atatcgggcg atttcctgta attgcttctg ttcttttgtc
     2041 ggcggtttcc atgcttttaa tttgtgcttt cggtcttggc aatattgggc tatcaacttt
     2101 gcgtcttgtg tatctgtttt tgatcgttgt agttctgcta tcgcatatcc ttttatcttt
     2161 cgtggattct ctacggtaat tgtatatctt gaataaagat attcggctaa tgcttcgtaa
     2221 tatgtgcctg ttgcttcgca cacgcaatgg agcttatcgt ttactttatg actttgtagc
     2281 caatttatta attgttcaaa tcctcctttg ttgttctgaa acttcttttg ataattttga
     2341 ccgtctacaa tcaaacagca atctattgtg agctttgaaa cgtctattcc taagtacatg
     2401 gtttaacctt attaattcgg gctttttgcc ctagatagtg ttcaaactta agatgtacga
     2461 aagcccacgc ttctatcttt cttacaagct gtacgctttg gccgtacttt cgaagtcgtg
     2521 ggctttactt ggtgtttcgt caaacgccaa gccctcaatg ggctgattta ctcattcagg
     2581 gcttgaagct tatcgccttt gccgtatctg attttattgg ggtcgcaggt tttggtaaag
     2641 gtttctgttg cgacccgaat gtctgatttt ttttgggcgt atctcagtct ggaatcactc
     2701 cgttcggggg tttccggtat tgaaaaacag ttcataaaaa aggaaaaggg ggtattcata
     2761 aagattgggt aaaaagcgcg cccaatcttt acaaagcttc ccccttttcc ttttttctgc
     2821 cctattttcc tgcacctaca acccccgaac gaagcgattc cagactgaga cactttaaaa
     2881 aaaacagcca ttctagcaat taaccccctt cactcagctc aagccatcct gaggggtagg
     2941 gattgaactt tctgctttac gactccgccg ccaattcctt caaacggttt tccgcgctct
     3001 tcagttgtcg tacatgagat tttgctacgg ctttccgccc attacgagaa cttgcggctt
     3061 gtccgctttc gcggactgtt ccacctgttc cgtcctttgc tgttcgtcct tataaggatt
     3121 gaaaggcaac ccgtttttca catattcttt acacattatc tttgttattt ctttcaaggg
     3181 tgttccttga tttgaatagc atgtgcaatc tgattttccg ccgtctatac atccggcgat
     3241 ttgctcaaag gtttttactt gtcggactgt gttataaata ggcttgcttt cgggcttttc
     3301 gggtaaagtc ggcacaaagt cttcaggttt caaattgtct gaatttttta aaggcatttc
     3361 ctctgatgat gcaggctgct ccgtcatcgt ctgcacaacg ctttcttttt gcgcttcctg
     3421 ctcaatccgg ctgtctgtgg ctttgctgta aactttaaaa atgccgtaac ttttccagcc
     3481 tacaaaccct acaatcgcaa tcaacgccca aaccgcccaa ggtacttttt tcttgaactt
     3541 ttggtgctgg cttgctgatt tatagtattt aaaggcttct ttcggcggtt tccaacttgc
     3601 gacttctacg ccgcttacgc ctgcgggatt gtccaacgag gttacgcatt tataccaata
     3661 atactgtttc attccgattg ccttgcgttc aaggtgtaca tgctttgaaa caaggttgcg
     3721 gacgaatata tcaagttggc tcgggtgctg cgtcatcaaa ataacggtat gcccgtgatg
     3781 gcggagttct gtcagttcct gaatataagg cggaacggga cggcctgccg cgcgtaccgg
     3841 ataagtgtag tgcgcttcgt caacaatcag cactgcgcct tccggtatga catcacgaag
     3901 cggggcggac atgatttgct cttccgtcag ttcgtgggct ttaaactgcc gtttatccaa
     3961 tccgtcgata tggcagaaat aaagcggtct gtctacctct gtgccgtctt ccaatttcat
     4021 tttgaacaat ccgtcttcgt tgttcaaaat catagagacg acgcgggagg ttttgcctgt
     4081 ccccatgttt cctgtaaaca gataaatcat gcttctacct catcctggaa agacaaacgt
     4141 cagttttttg aatgcgtgca taccaatgaa gaacgagaat gcgccgaaca ggtagcccaa
     4201 cccctgaccg aatcccgaaa ttaaaagaag gttcagtatg tcggaaggca tggaattgat
     4261 cgcatttgac gtgtagtctt tgaacttttc cagcgcgatg agatacccgg cataggttac
     4321 aaatgtcaga cctgttgcaa ggattattct gacaatcagc attttcagaa gtatgcctaa
     4381 aagtggaatc agaccggcaa gtaatggcat ttatttcccc ttcaacgaac cgaaaacgac
     4441 aaaagccgac ataatgataa aggcgagcag tacggcaaaa cggatttttt cggcaaacac
     4501 gcataacggc tcatagcttg cctgatattg cctaccgaaa acatgaaagg ttttcggctg
     4561 cggacatacg ccgttagacg gtaaaaagtt atgtgaagac catgttttat cgtctataac
     4621 ctgcggtatg cttatatcgt gaaacatgcc gtctgaaggt ttgcccatct cctgacaggc
     4681 taggatttcc ggaaaataat cgcacaaaag cccgccgtct tcgccttctt tcctttcttt
     4741 gcgatgcct
//
LOCUS       DAXHPG010000153         4731 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXHPG010000153 DAXHPG010000000
VERSION     DAXHPG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.153"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            1..609
                     /locus_tag="ACFM6F_001814"
                     /pseudo
     CDS             1..609
                     /locus_tag="ACFM6F_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153708080.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(745..2148)
                     /locus_tag="ACFM6F_001815"
     CDS             complement(745..2148)
                     /locus_tag="ACFM6F_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFC6165182.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(2217..3644)
                     /locus_tag="ACFM6F_001816"
     CDS             complement(2217..3644)
                     /locus_tag="ACFM6F_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFC6165183.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSVQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3880..4233
                     /locus_tag="ACFM6F_001817"
     CDS             3880..4233
                     /locus_tag="ACFM6F_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165184.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            4357..>4731
                     /locus_tag="ACFM6F_001818"
     CDS             4357..>4731
                     /locus_tag="ACFM6F_001818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165185.1"
                     /translation="MGNTAMWMSCNPTIPILFGIQVKIFPLNQILNHIHPARYAVTFE
                     NNVDSKLRRHWVAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAILCNK
                     ERLTSSEPLSDFVLSVLKPYILR"
BASE COUNT         1139 a         1236 c         1153 g         1203 t
ORIGIN      
        1 accgtttgct atgcactcaa acagatgggg ataacgcgca aaaaaagacc accacttaca
       61 aagaacaaga cccggccaaa gtaacgcatt atttgacaca gccggccgaa ttttctgact
      121 accaacgcgt ttatttggat gaaacaggat ttgaccgcca cctgttccgt ccctatgccc
      181 gcagcctgaa agggcaaata gtgaaagcgc agataagtgg aaaaagatac cgacgcttat
      241 ctctggtgtc cgcacaagtc ggcaaccggc tgattgctcc gatggtttat caaaatacga
      301 tgaccggagt cttttttgaa gcgtggtttc agcaatgcct actgcccgca ttgactcaaa
      361 aatcggtgat tattttagat aatgcgcgat ttcaccgtat gggtgtctta cgggaaatgg
      421 cggaaaaatt gggacataag gtattgcctc ttgcacctta ttcacctgag ctcaacccga
      481 ttgagaaagt gtgggcgaat attaagcggt atctgcgaac cgttttgtct gattacgccc
      541 gatttgacga tgcactactg tcctattttg attttaattg aatatacatt tgaattaatt
      601 cgcacttaat ttaaatgtgt ttttaactgt gctttattta aaggcaatga gaatgtgaaa
      661 gtatcggatc aatcccaaag cagcctgcac tttcgaaacg gggtgcaggc tgctttggga
      721 atttcataac cgtttcagcc tgctttattc cgcaaatacc gtttccaacc ctaacccgct
      781 ctctttcacc aagcgcaaat aagccagcat gaatttatac cgtgcttgag ccagtttctg
      841 ttctgcttgg gcgacttcct gccgcgcccg tattacttcc agccggttgc ggatgccgta
      901 ttgttggccg gtttcggtcg atttcagttt caaacggctg ctttccaaaa cccgttcttg
      961 cgccatgatt tggtaacgcg ccgcaccgct ttcggtataa gcctggcgta cggcgagttt
     1021 gatgtgccgc tcggttgcgg tcagctgtgc ttcggcggcc ccgtattgcg cttcggcttc
     1081 atggattttg cccgacaatt ctccgccggt ataaagcggc aaattcaact gtacgccgac
     1141 gctcatccct ttgccccgat agtggtagtc attattctgc gcagatgaag tgtagaggtt
     1201 attctgatag ccgacatggg cagaaacggt gggatagcgg ctgttctgtg ctgcccgaag
     1261 cgcctgtccg ctgctttgca gggcaagctg ctgcatccgg tattcatgat tgttggataa
     1321 ggcaatgcgc tgccattcat ccagactgta acgttccagc ttgggcagat agcgtgccaa
     1381 caggttggcg gtatctatgg cctcgatttg tttgctatcc aggtcggtgt agtcgttcaa
     1441 ctggttttca taggtttgtt tctcagccaa tacggcgatt tcttgggcca gggcattgtc
     1501 gtaaccggct ttggcttcgt gaatatccag cgcggtggca gcacctttat tgaataaagc
     1561 ctgcgcctgc cttacctgct gggcataagc ctctttttcc gccgcatggg cggcaacggt
     1621 gtctcggctg agtaaaacgt tgaaataact ttcggcaact ttcaacagca attcttcgcg
     1681 tgccgcatcg aaacgctgtt ctgcagcctg cgtatcgaac ctgctttggc ggtattgtgc
     1741 aaatttggca gcgtcaaata aggtttgtcc cacctgcacg ctccatccct gtgtttcgcg
     1801 ggtggaagaa atcgatggcg gctggcgctg gtagctggca ttggcggata catggggaag
     1861 gaatgcggcc ttggcttgtt gttgccgtgc gcgcactgca tcacgctggt aatgggacgc
     1921 ttgaaaatca gccgaatgtt gctgcgccgc ccgccatgct tcaggcagcg taaaagccga
     1981 aacggatggg gaaagggata gtggcaaggt aaaaagtgaa acgggtagga tatatttgga
     2041 aaaataggat ttcatagccg aaaatagttc atgttgcaaa tagggcgtca gtgtcaggca
     2101 aacggaaata ccgtaatctt gcattatcat tagattgagc aatgtcatcc gggcaatggt
     2161 ttcaggcagt ctgcatgtcc gaaccggcgg ataacaaatg cccagtacgg atccgcctat
     2221 cgctccctaa agctttcgtc caatttggtt tgcagcgggc ttaacagata atccagcacc
     2281 cgccgtttac ccgttttaat ctccgccgtg acattcatgc ccgccgtcag attcactgct
     2341 ttgccgtcaa tattcaaggt atgtttgtcc agcgacacca ccgccgtata aaccaagccc
     2401 aactgttcgt ggcttaccgc atcatggctg acacttttca ccttgcccgt cagataaccg
     2461 tagcgcgtat agggaaagct ctcaatcttc accaccgcat cctgtccctg ttccacaaaa
     2521 ccgatgtctt tgttcaatac caaaacttcc acgtccattt tgtcgtcatc gggcgcaatc
     2581 accatcattt tttgggcagc ctgcaccaca ccgcccaccg tataggtagc caattcctgc
     2641 accgtgccgt ccgcaggcga ctgtattgtc atcagctgct gccgctgctt tgccttatcc
     2701 gtttggccgc ggtattggtc aatctgttcg tttgcctggc gcagcgcatc cagcgtatcg
     2761 cgtttcaggt tctgcgtatt cagcacccga ttctgctccg cctgtgcaat ggccgcctga
     2821 atctgcctca tctgaccgcg cgtactttcc aaatcgttcc aattgctgac cgatttgctc
     2881 tgctgctcca aaaacgcatg ttccgaaata aaattgtcgg cccgcaaacg gcggtagtct
     2941 gctgttttct gctgctcgat cgcccccacc gaaaccagct tctgctcctg cgccttggcc
     3001 gactgcaatt ccgcctgatg gccgcgcaaa gccgactgca attgcgcatc ctgcgccgcc
     3061 catgcctgat actggtgctg cgccaacacc tgcaccgatt gcacatcggc atcggagaga
     3121 cctaaagacc gtgcttgcgc catatcgata tgcggcacgg tacggctttc caatgccgcc
     3181 aataccgctt cataacgcag tttggacaat tgggcagcct gcaaagcctg ctccgactgc
     3241 accacatcgc tgtctgttcc cacagcctcc agttccgcca gcgtttctcc ctgtttcaca
     3301 tgctgcccgt cgcgcacatg taccgcctta accaccgccg tttccagcgg ctggatggtt
     3361 ttgctgcgcc cgcccgacac cgttttgccc gaagccgccg ccacaatatc gattttgccg
     3421 aaccaggacc acaacaaagc caaaagcgca aacgccataa taaaacgcgc cgcccatttc
     3481 ggagcggcag agaccggcgt atcggtcagt tccaaatgcg cgggcaaaaa cgcctgttct
     3541 tccgccgtgc gtttgggcgg tttcaactgg tcgcgcaccg cccaaacatt gcgccataca
     3601 gtaatgtatc gagaaagaaa ggatttcagg gcggagaaaa acataacggg tataaccttg
     3661 gcaatataga aacaggaaac aatataaata tgtaaaggaa ttttaacgga aagcgcggca
     3721 gctgttaagg gaaaggcggg aatattgaca aaaaataccc aagtcgttac aaatattcat
     3781 tattttactg cgtaacgcaa cgctgaagcg caggctgctt ttgagatgcg gcaaggttcg
     3841 gcaaaaagca gcctgcacat ttaaccacag gaacaaccca tgtttaccac aaacgattta
     3901 cgccatttcc tagaaggttt ggccatccta ttctcaatcg gctattgggg caccatgctg
     3961 ctgttgcttt ggtttctcgt ccgctttgcc tataaaaagc ccaaacggaa ccccggcaaa
     4021 atcctttcag gcagcctgac cgccgccgct atcctgatgc tgtttgtttg gattattccc
     4081 aaacaattcg gcccgatcaa agaagaaata caggcacaag aagagtggga cagaaaatac
     4141 aaagaagccg aagccgtgtt taacgaacaa tgcaaaacgg cgggggaaag atttaccaga
     4201 cggcggacaa tgtggaaggg attatgctgt tgaaggtagt acctgagcgt accgtttcgg
     4261 cagatgcaaa aaccagagac ccgatgtggg acaatgcggc tttacagacc agcgaaggcg
     4321 taaattttat tgctcgtttc ctaggatttt ttagcgatgg ggaataccgc tatgtggatg
     4381 tcctgcaacc caaccattcc gatattattc ggtattcagg taaagatttt tccgctaaat
     4441 caaatactta atcatataca ccccgcccgt tatgcggtaa cgttcgaaaa caatgtcgat
     4501 tccaagctgc gcaggcactg ggtggcaggt gcgaccatac ggattatcga ccgccaaact
     4561 gacgaagtga ttgccaagaa aaccatctat gtctttgaaa aaggcttgga cggcacgggt
     4621 ggggcgagaa tgccgtggaa gtttgctatc ttgtgcaata aagaaagact tacttcttca
     4681 gagccgttat cggattttgt tcttagcgtt ttaaaacctt atatattgcg t
//
LOCUS       DAXHPG010000154         4687 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXHPG010000154 DAXHPG010000000
VERSION     DAXHPG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.154"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(416..952)
                     /locus_tag="ACFM6F_001819"
     CDS             complement(416..952)
                     /locus_tag="ACFM6F_001819"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC6165186.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(986..1342)
                     /gene="folB"
                     /locus_tag="ACFM6F_001820"
     CDS             complement(986..1342)
                     /gene="folB"
                     /locus_tag="ACFM6F_001820"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC6165187.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1398..2000
                     /gene="plsY"
                     /locus_tag="ACFM6F_001821"
     CDS             1398..2000
                     /gene="plsY"
                     /locus_tag="ACFM6F_001821"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC6165188.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALVWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNIVKLLEGRESKIGGSR"
     gene            2077..2922
                     /locus_tag="ACFM6F_001822"
     CDS             2077..2922
                     /locus_tag="ACFM6F_001822"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC6165189.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRHVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3168..4142
                     /locus_tag="ACFM6F_001823"
     CDS             3168..4142
                     /locus_tag="ACFM6F_001823"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC6165190.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYILYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(4285..4503)
                     /locus_tag="ACFM6F_001824"
     CDS             complement(4285..4503)
                     /locus_tag="ACFM6F_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC6165191.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            complement(4479..>4687)
                     /locus_tag="ACFM6F_001825"
     CDS             complement(4479..>4687)
                     /locus_tag="ACFM6F_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165192.1"
                     /translation="GKAENLCVKRDIVNVFINRTAVLKENFAVSDKTLDKNIIFVIIN
                     VKIQFDFLTVNQERKAICSQNTVI"
BASE COUNT         1207 a         1402 c          984 g         1094 t
ORIGIN      
        1 ctcaaattca aattgggctt ttaggtctat aacaaagaaa aagccttggt aggataaacc
       61 taccaaggct ttttcttaaa aaaacgctct attaccgtgc ctaaaaatgc aaaaaagtaa
      121 ccgtcataac ccttttgcca aaatcaatat catcatccga tgaatgaatt gacggtatat
      181 aagaaagaat taataagctc ataaaccggt aagaaaaatc agtaacaaag atattcatta
      241 ttaaccattt taaaaattat catgttcgtt tgggtaatta ttataccctt ttccctctta
      301 ttactaaagc acgaatgact ttaaaggacg cgcccagaga caagccgaaa aggcatatct
      361 catccgccaa acaaaatgcc caccgactta ctccggcagg cagttcaaca ccctgtcaat
      421 ccttcatcag ccagtattgc aaaccgatta atgtcttgcc gtctttaatt tcatcgtttg
      481 ctaatgcctg acggacttct tctttcgaca tcaatacggt ttccgtaatc tcgtcttcgt
      541 cattggcaag cgtactgccc aaacgcacgc cttccgcttc gaacagatac attttttcgt
      601 tgcaaaaacc gaccgccgta taaaaactgt aaagcaggcg cacgctgtcg gcaacataag
      661 gcgtttcttc agccagttct ctcagcgcac acgctgccat atcctcgccc gccacatcca
      721 gcttgcccgc aggaagttcc aatgtcgcct gatttgccgc ataccgccac tgccgtacca
      781 aaacgacctt ttcatcatcc gttaccgcca acacgcacgc cgctcccgga tgacggatga
      841 cgatacgctg cccttcattg ccgttgggca acctgacctt atccctgctg atgctgacaa
      901 aaccgccctc gtaaatggtt tcgccgccta attttacttc cctcaaatcc acttcatatc
      961 tcctttccgt ttcctattct gccgttcaat cttcacgctt gccgcgctcg atttccacgc
     1021 caacctcgcg cacgccttcc aaaatacccg gcttgacgat tttcacgcgt acccacaccg
     1081 cgccgaaata tcccaaaacc aaatcggcaa tatattccgc caacgcttcc aaaagcagga
     1141 aatcctgttc tttcagatgt cggcgcagtg tttcgcatac ctcggcataa tgtaccgtat
     1201 tggcaatatc gtcgtccgaa cccgctttct cgggaacgcc gatgtccaaa tcgacaatca
     1261 gggtctgcaa ccgttcgcgt tcccagccgt acacgccgat aagcgtatcc gccttgatgc
     1321 cgtgcaaaaa gattttatcc ataatccgcc ccgttttttg ctaaaataac cgcaccattc
     1381 taagtaaagt accacaaatg ttcaacatac cggctgttgc cgtttcctat ctgatcggct
     1441 cactttcttt cgccgtcatc gtttccaagt attacgggat ggacgacccg cgcacctacg
     1501 gatcgggcaa tcctggggcg accaatgttt tacgcagcgg caaaaaaaag gcggccgcgc
     1561 tgacgctctt gggcgatgcc gccaaaggtt tagttgccgt tttgcttgca cgcgtgcttc
     1621 aagaaccgct cggtttatcc gacagcgcaa tcgctgccgt cgcactcgcc gcgctggtcg
     1681 ggcatatgtg gccggtgttt ttcggattta agggcggcaa aggcgtggca acggcattgg
     1741 gcgtgcttct ggcactctct cccgcaactg ccttggtctg cgcgttggtt tggcttgtta
     1801 tggcattcgg cttcaaggtg tcctcccttg ccgcattaac cgccacaatc gccgcccccc
     1861 ttgccgcact gttttttatg ccgcatactt cttggatttt cgcaaccctc gcaatcgcca
     1921 tattggtgtt gctccgccac aaaagtaata tcgtcaagct gctcgaaggc agagaaagca
     1981 aaatcggcgg cagccgctga tttcccatcc atccctgttt atgccgtctg aaccccgctt
     2041 cagacggcat ccgaatatcc cgccgtttta ccgactatgc tcagttacag acacgcattc
     2101 cacgccggca accacgccga tatgctcaaa cacttcacct tgtttttagt gctgcaatat
     2161 ttcaaccgca aagacaagcc ctactggtac atcgacacgc acggcggcgc gggtgtgtac
     2221 aatctcgaag gcagcgaggc acaaaaagtc ggcgaatacc ggcaaggcat cgccctactt
     2281 cgacaagcaa aaaacctgcc aaccgagtta aacggcttta tcgcgcacat acaaaaaatc
     2341 ctgccctcgc ccgaacttta ctgcggttct ccgtggctgg cgcaatcgct gacccgcgtc
     2401 ggcgacaaat tacgcctgtt tgagctgcac cccaccgact ttgtccatct gcaaaacaat
     2461 atgggcgaag caggattggg caaacgcgga caagtgttgc gcgaagacgg ttacaaaggg
     2521 ctgatttccc tgctgccgcc cccgccgcgc cgcgccaccg tcctcatcga cccgccctat
     2581 gaggaaaaac aggactaccg gcatgtaacg gaaacgctga aagccgcttt aaaacggttt
     2641 gagtccggct gttatctcat ttggtatccc tgcctcagcc gcgaagaaag ccgcaagctg
     2701 cccgaagaat tgaaaaaact cctgcccgac aactaccttc acgccgaact tcacgtccac
     2761 gcgccgaaag ccgacggttt cggtatgcac ggcagcggaa tgttcgtcat caatccgccc
     2821 tatctgttgg cggaacaatt ggcggcaaac ctccccgccc tgacgcggct gctggcgcaa
     2881 gacgaaggcg cgcgctacct tttggacagc aaaatccgct gaccccgatg ccgtctgaaa
     2941 cccttcagac aacatctccc gtcaaataaa caaaaccgga cgatataccg gcagtcggca
     3001 cttttaagaa taaatatccc ctcatccgcc tgccgcgttc cgacttgccc catgccgttt
     3061 ttttacacta caatccccta gactatcctg caaaacgcac caaaggaata cgccacaata
     3121 acctgccgcc cttcagacgg cacaaacctt tccgaaacga gcaaactatg gacacactga
     3181 cccgattcct ccccgaacac ctgcaacaaa accagctgcc cgaagcactc ggcggcgttt
     3241 tactgtccgt tgtttccgcc tgcaccgaaa tcaacgccaa agtccgcctc ggcgcactgg
     3301 ccggcgtatt gggtatggcg ggcacgggca atattcaggg cgaagaccaa aaaaaactgg
     3361 atgttatcgc caacaacatc atgattgaca cactcaaagc caaccctgcc gttgccggtt
     3421 tggcaagcga ggaagaagac tctttcgtaa gtgccggtga aaacggacgc tatctcgtcc
     3481 tattcgaccc tttagacgga tcgtccaata ttgatgtcaa catttccgtc ggtacaattt
     3541 tctccatcct tgaaaaaccc gaaggcgcat tggcaaccga atcattcctt caaacgggca
     3601 gacagcagct tgccgcaggc tatatcctct acgggcctca aacccagctc gtattcacat
     3661 tcggacacgg cgtatatatg ttcacgctca atgccgaaaa cgaatttgtg ctgaccaaag
     3721 aaaaaccgaa agtacccgaa agtaccaaag aatttgccat caatatgtcc aaccgccgcc
     3781 actggctgcc ccccgtccaa caatacatcg acgagctctt ggcgggcgaa accggcacgc
     3841 gcggcaaaaa ctacaatatg cgctgggtag ccagcatggt tgccgaaatc caccgcatcc
     3901 tgatgcgcgg cggcgtgttc atgtatccgc aagacaaacg cgacccgtcc aaacccggca
     3961 aactgcgcct gatgtacgaa gccaacccta tgagcctgat actcgaacag gcgggcgcat
     4021 cggcaagcaa cgcatatcaa gccatgctcg acatacagcc cgaaaacctg caccaacgcg
     4081 tcgccgtctt catgggcagc agcgaagaag tcgcctatct cgaccgcctg cacgcaaaat
     4141 agacagacag ccgttcacac aagtacacgg cggcggcaat ccgataccgc cgccgttatt
     4201 tcaaacagca caagccttct ggtcaaatga caaaaatgcc gtctgaaaac atttcagacg
     4261 gcatttcaaa aactcaaacc cgaattattt tcccgctgct ttttgcagga tggcgtacaa
     4321 ctcgtctttc agtttcaatt tggctttttt cagctcatcg atggtttccg caccgctggt
     4381 aaccggattg ttgaccagac cggtaatttt atcgtccagc tcgttgtgtt cgtcgaacag
     4441 acgggcgaag cgggaatttt cctgtttcaa tttggaaatc aaatcacggt attctgggaa
     4501 catatcgctt tcctctcttg gtttacggtt aaaaagtcaa actgaatctt tacattaatt
     4561 ataacaaaga taatattttt atccagtgtt ttatcggata cggcaaaatt ttcttttaac
     4621 accgccgtcc ggttgataaa aacgttgaca atatcacgct ttacgcataa attttcagcc
     4681 tttccta
//
LOCUS       DAXHPG010000155         4672 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXHPG010000155 DAXHPG010000000
VERSION     DAXHPG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.155"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            119..1294
                     /locus_tag="ACFM6F_001826"
     CDS             119..1294
                     /locus_tag="ACFM6F_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC6165193.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGEGDDVVVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAIIVVHLAGMPAEMDDIMALAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVSELNARKVPCYQGSCSEV
                     YLEKSFDDTPWRPKERLKNAVELGDTSLMFLVHPTLTDDEIAFCKEHIEAVLVEATR"
     gene            1342..3252
                     /gene="pglD"
                     /locus_tag="ACFM6F_001827"
     CDS             1342..3252
                     /gene="pglD"
                     /locus_tag="ACFM6F_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HFC6165194.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAFLTACLFFRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMAPVIIYGAGQAGRQLLEA
                     VKQMREYSAAAFVDDDPKLWRTVICGLAVYRPDDIAALIGRYGVEKILLAIPSATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVTPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAVEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPDITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            3308..4600
                     /locus_tag="ACFM6F_001828"
     CDS             3308..4600
                     /locus_tag="ACFM6F_001828"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC6165195.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHIEGQHFVSAKPK
                     IENVEHEGEAGFFKYRVNFDALENLPELKEGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAQKHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEILKAEGTLIIPGEHFFIGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
BASE COUNT         1136 a         1497 c         1145 g          894 t
ORIGIN      
        1 cacccccgta cagaccgatt ttgacaacac ctgcggcgcg cgcccgattc ctcgaaacac
       61 gcccccttca gacggcatag ggtcggaaat gccgtctgaa cccgacggac aaaccatcat
      121 gttgaacact tccctttccc cgtggccctg cttcacccaa gaagaagccg atgccgtttc
      181 caaagtcctc ttgtccaaca aagtcaatta ctggacgggc agcgaatgcc gcgaatttga
      241 aaaagaattt gccgcctttg ccggcacgcg gtacgccgtc gccctttcca acggcacgct
      301 ggcactcgat gccgcgctca aagcaatcgg cataggcgag ggcgatgatg tggttgttac
      361 ctcgcgcacc ttcctcgcct ccgcgtcctg catcgtgaac gcgggcgcaa accccgtgtt
      421 tgccgacgtg gatttgaaca gccaaaacat cagcgcggaa accgtcaagg ctgtgctgac
      481 cccgaatacc aaagcgatta tcgtggttca ccttgccggt atgcctgccg aaatggacga
      541 catcatggct ttggcaaaag aacatgattt gtgggtaatt gaagactgcg cccaagcgca
      601 cggcgcaaaa tacaaaggca aatccgtcgg ctctatcgga cacgtcggcg cgtggtcgtt
      661 ctgccaagac aaaatcatga ccaccggcgg cgaaggcggt atggttacga ccaacgacaa
      721 aaccctgtgg gaaaaaatgt ggtcgtacaa agaccacggc aaaagctacg atgccgtgta
      781 ccaccgcgaa cacgcgcccg gtttccgctg gctgcacgaa agtttcggca caaactggcg
      841 tatgatggaa atgcaggcgg caatcgggcg catccagctc aaacgcctgc ccgaatggac
      901 tgcctgccgt caagcgcacg ccgccaagct ggcggaaagc ctaggcaaat tcaaaagtat
      961 ccgcttggtt gaagtcgccg actacatcgg acacgcgcaa tataagttct acgctttcgt
     1021 caaacccgaa cacctcaaag acggctggac gcgcgaccgc atcgtcagcg aactgaacgc
     1081 gcgcaaagtc ccctgctatc aaggcagctg ctccgaagtc tatttggaaa aatctttcga
     1141 cgacacgcct tggcggccga aagagcgttt gaaaaatgct gtcgaactgg gcgacaccag
     1201 cctgatgttc ttggtgcacc cgacgctgac cgacgacgaa atcgcgttct gcaaagaaca
     1261 catcgaagcc gtattggtag aagccacacg ataacccttc agacggcata tgccgcctga
     1321 aagcacatac cgccccacga tatgactctg gaaaccctga tcgccctgcc gcgcaacatc
     1381 aagaaaatct gtttcctcat acacgatttt ctgatgattt tcattgcatt ctggttcacc
     1441 caaagcctaa aggccgacta ctcgaacgaa tggttcgacc ccgccaactg gcagtctttc
     1501 ctattgaccg cgtttttgac cgcctgcctg ttcttcagaa tggggcttta ccgcgccgtc
     1561 acacgcttca tcagcttccg catcctctcc accgcgctgg caggcagcct cgcctccgcc
     1621 gtattgtttt tcctcaatac gctgatattt gaagaacggc tgcggctcgc cctgccgatt
     1681 gtctatttct tactgctgtt tgtttccgtg accggctcgc gtatggtttt gcgcggactg
     1741 ttgtccgaac accccaaaaa acagatggcc cccgtcatta tttacggcgc agggcaggcg
     1801 ggcagacagc ttctcgaggc cgtcaaacaa atgcgcgaat attccgccgc cgcctttgtg
     1861 gacgacgacc ccaaactgtg gcgcaccgtc atctgcggcc ttgccgttta ccgccccgac
     1921 gacatcgccg ccctcatcgg acgctacggc gtggaaaaaa tcctgctcgc cattcccagc
     1981 gcgactcagg aacaacgccg ccgaatcatc aacaaactgg aagcctatcc gtgcgaagtg
     2041 ttgaccattc ccggaatgaa agacctgatg gacgggaaaa tcagcatcgg cacgctcaaa
     2101 aaaatctctg tgtccgacct gctcgggcgc gattccgtca cacccgacga ccgcctgatg
     2161 agtgccgaca tcgaaggtaa aaccgtcatg gtaaccggcg cgggcggctc catcggttcg
     2221 gaactctgcc gccagattat ccgccgccgc cccgaaaagc tgctgctgtt cgagttatcc
     2281 gaattcgccc tgtacgccgt cgaaaaagaa ttgcgcgaaa cctgcatcca aaaacgcctc
     2341 gacaccgaaa tcctgccctt tctcggttcg gtgcaaaacc gcacgctgct cgaacacgtc
     2401 atgaccgcct tttccgttgc gaccgtctat cacgccgccg cctacaaaca cgtccccatg
     2461 gtcgagttca acaccgtcga aggcatacgc aacaacatct tcggcacact cgaatgcgcg
     2521 cttgccgcca cggcttcggg cgtggaaacc ttcgtcctca tctccaccga caaagccgtc
     2581 cgccccacca acacgatggg cgccagcaaa cgtatggcgg aactctgcct acaggcgctc
     2641 gccgccgaac ccggacaaaa aacccgcttc agcatggtgc gtttcggcaa tgttttaggc
     2701 tcgtccggct ccgtcgtccc gctgtttgaa aaacagattg cagaaggcgg cccgcttacc
     2761 ctgacccacc ccgacatcac acgttatttc atgaccatac ccgaagccgc ccaactcgtc
     2821 atacaggcag gcgcgatggg tactggcggc gacgtattcg tcctcgatat gggcgaatcc
     2881 gtcaaaatca tcgaccttgc ccgccaaatg attaccttaa gcggcctcaa acccaaaaca
     2941 cccgaacaac ccgacggcga catcgaaatc ctcattaccg gactgcgtcc cggcgaaaaa
     3001 ctctacgaag agctgctcat cggcgacaac gtccgcaaaa ccggccatcc gcgcattatg
     3061 acggctgacg aggcgatgct gccgtggcac gagctctctg tcctgctcga ccgcatccgt
     3121 gcggcctgcg accgttacga ccagcaggca atccgcaccc tgctcatcaa cgccccgacc
     3181 ggctttgccc cgagcgacgg tatctgcgac ctgctttggg tacgagaaac acacagaaaa
     3241 aatgccgtct gaaccttcag acggcataac gtacaaacca acctacctta cacacacgga
     3301 gtttgacatg cagttttcgg cattcggcga aaaattcacg caacacagcg gcatcctcca
     3361 actgatggac gacctcggcg acgcgctcaa aagcgacaag cccgtcaaca tgctcggcgg
     3421 cggcaacccg gcgcgcattc cggaaatcga tcgggcgttt gccgacatat tctccaaact
     3481 ggcggcagaa cacgccgtcg aaaacatcgg caactactcc aatcctcaag gagatgccgc
     3541 gctgattgac gcgctaaccg ccttcctcaa ccgcgaatac ggctggaacc tgaccgccga
     3601 caatatcgcg ctgaccaatg gttcgcaaaa cgcgtttttc tatttattca acctcttcgg
     3661 cggcaaattc aagctttcag acggcacatc cgcagaaaaa gccattttgt tgccgctcgc
     3721 gcccgaatac atcggctatg ccgacgtgca tatcgaaggg cagcacttcg tttccgccaa
     3781 acccaaaatc gaaaacgtcg aacacgaagg cgaagcaggc ttcttcaaat accgcgtgaa
     3841 ctttgacgca ctggaaaacc tgcccgagct gaaagaaggc aaaatcggcg cgatttgctg
     3901 ttcgcgcccg accaacccga ccggcaacgt gctgaccgac ggcgaaatgg cgcgtttgga
     3961 cgctttggcg caaaaacacg gcattccgct gattatcgac aacgcctacg gaatgccgtt
     4021 ccccaacatc atttacagcg acgtaacgct gaattggcat gaaaacatca tcctctgctt
     4081 cagcctttcc aaagtcggtc tgccgggcgt gcgcaccggc atcatcgtcg ccgcgcccga
     4141 agtcgtcaaa gccgtcagca gcctgaacgc gattgtgaac cttgccccca cgcgcttcgg
     4201 cgcggccatc gccgccccgc tgctggaaag cggcgagatg aaacggcttg ccgaccaagt
     4261 catccggccg ttttaccgca atcaggcgca aaccgccgtc tcgctgctca agcgcgagct
     4321 gggcgcgtac ccgatgaaaa tccacaaacc cgaaggcgcg attttcctgt ggctctggtt
     4381 tgaaaacctg cccgtttctt cgcaaaccct gtacgaaata ctcaaagccg aaggcacgct
     4441 gattattccg ggcgagcatt tcttcatcgg catcgacacg caggattacc cgcacgcggg
     4501 cgagtgcatc cgcatgagca tcgcgcagga cgctcaaacg ctggaaaaag gcattgccgc
     4561 catcggcaaa accgtccgaa aactgtacga caacgtttaa aacgcaaaaa aatgccgtct
     4621 gaaaagtttt cagacggcat gagacctttg caataacata ggttactaaa at
//
LOCUS       DAXHPG010000156         4665 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXHPG010000156 DAXHPG010000000
VERSION     DAXHPG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.156"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..857)
                     /locus_tag="ACFM6F_001829"
     CDS             complement(<1..857)
                     /locus_tag="ACFM6F_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6165196.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTLSHKIDQRAFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDEEGNWSDVGLSSASFLGGLMGFQWGAALGVGAFGLLTGGAGWLVVGT
                     IIGGGSGAWWGSLGAEKAWRWFEDFLNLNRSDKFHVTDPLALDLDGDSIETVATKGLA
                     GALFDHRSQG"
     gene            2035..4263
                     /locus_tag="ACFM6F_001830"
     CDS             2035..4263
                     /locus_tag="ACFM6F_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFC6165197.1"
                     /translation="MCSAVLFIYFFLLNIFLFQPAVIHIQSNYILFMSIVSAPLPALS
                     ALIILAHYHGIAANPADIQHEFCTSAQSDLNETQWLLAAKSLGLKAKVVRQPVKRLAM
                     ATLPALVWCDDGNHFILAKTDGGGEHAQYLIQDLTTNKSAVLSFAEFSNRYSGKLILV
                     ASRASVLGSLAKFDFTWFIPAVIKYRRLFFEVLVVSVVLQLFALITPLFFQVVMDKVL
                     VHRGFSTLDVVSVALLVVSLFEIVLGGLRTYLFAHTTSRIDVELGARLFRHLLSLPLS
                     YFEHRRVGDTVARVRELEQIRNFLTGQALTSVLDLAFSFIFLAVMWYYSSTLTWVVLA
                     SLPAYAFWSAFISPILRTRLNDKFARNADNQSFLVESITAVGTVKAMAVEPQMTQRWD
                     NQLAAYVASGFRVTKLAVVGQQGVQLIQKLVTVATLWIGARLVIEGKLTVGQLIAFNM
                     LSGQVAAPVIRLAQLWQDFQQVGISVARLGDILNAPTENASSHLALPDIRGEITFEHV
                     DFRYKADGRLILQDLNLRIRAGEVLGIVGRSGSGKSTLTKLVQRLYVPEQGRVLVDGN
                     DLALAAPAWLRRQVGVVLQENVLLNRSIRDNIALTDTGMPLERIIEAAKLAGAHEFIM
                     ELPEGYGTVVGEQGAGLSGGQRQRIAIARALITNPRILIFDEATSALDYESERAIMQN
                     MQAICANRTVLIIAHRLSTVKTAHRIIAMDKGRIVEAGTQQELLAKPNGYYRYLYDLQ
                     NG"
     gene            4487..>4665
                     /locus_tag="ACFM6F_001831"
     CDS             4487..>4665
                     /locus_tag="ACFM6F_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6165198.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLS"
BASE COUNT         1202 a         1051 c         1052 g         1360 t
ORIGIN      
        1 ccttggctgc ggtggtcgaa caatgcacct gcaaggcctt tggtggcaac ggtttctata
       61 ctgtcgccat ctagatctag ggcaaggggg tcggttacgt ggaatttgtc gctgcggtta
      121 agatttaaga aatcttcaaa ccatctccaa gctttttcag ctcctaatga tccccaccaa
      181 gctcctgagc cacctccaat tatagttcca actactagcc aacctgcgcc accagtaagt
      241 aaaccaaaag ccccaacacc caaagccgca ccccattgga agcccattag cccacccaaa
      301 aaagaagctg atgagagacc aacatcactc caatttcctt cttcatccaa ggctttccac
      361 caagcacttc ccatactcaa agttcccatg gcaacagttg ccccataagt aaacttaccc
      421 aaattcgctg ccataacttt tgattttgaa gcattctcta acgctgttgc cgcacgtctt
      481 gcatattgct gcttgttata gttgctagca ttcaatgctt tttgcaaatc actttgtact
      541 tttcttgcaa caaatgcctc ctttgaatta ccacgaaatt tactagcagc tcttccaatt
      601 ccttctttta agcttttttg tgcctcttct gctgtgcgtt ggaattctct tgccttagca
      661 gacagacgct caccctccct taaaaatgct cgttgatcta tcttgtgcga aagggtcaaa
      721 tattcttcgt acattgagtt aatcgtacca atcgccctaa ccacattttc atcagaaaat
      781 atggaaataa tagcatccct atacgcacct agagtaatat tgtttctatt attagttata
      841 gcattattcg aatacataat agcacctcca aacttagaac tgaatacgtt tatattctct
      901 gtgtaataat atcaaataat taattaatgg ataagtaagt ccgaaaaaat aacaactaaa
      961 ataagcaata tactgattct ctcctttatt aattagatat attgaaaaac aaataaaaaa
     1021 taaacagaaa acattcatgt gaaaaagcca cctcatattc ttatttataa gtttaaaata
     1081 actaggcatt gtatatttaa aaatcggtac atccaacgga aacaaaccac ataaaaattt
     1141 ttctttattc tttattaaag acatagtttt atttttcaaa attagtccag ataatatact
     1201 aacaattact attaaaacaa taagcacagc gtaatttttt ataaaattca ttaaaataat
     1261 aacgaaaata ctaaatagat tacatactgt aaattgaaat ataaaatatt cagtatttaa
     1321 tccattttca accgcgagtt ttttaaaata tccttgctcg aaaaaattaa aaaaatccat
     1381 taatactttc ctctcaagaa aactaatttt agtttagtta agataaaatg caatattacc
     1441 ttttctccat aaaagtatgg agcttatata cataaattat gtgcgaggta gcctgaaaaa
     1501 tatccaatac aagatagtag gagatgtttc ccttatcact attggccaat acccaattgg
     1561 caggcactct atttccaaaa ttttctcaag aatagttaaa tattattaat tagtttaaaa
     1621 acaataacat acgtttgtat ttatccgttt tggtctgcct gaaacagact ctatgtccat
     1681 acacggacat gtagacagtt tccttgttat tttggttgct cgtcattagt gatgatactt
     1741 tactcctctc ttaaaaaatc gtcaagaact gttatcaatc tttaaagtct tttttaaaca
     1801 aatatatata cgttcaggct gtctgaaaca caaaaaaatg atgttacccg accgtgacag
     1861 agtcggggca gccggtacct taaccctgca ccttaaccct gtgccttaac ggtatgcagg
     1921 aggcacgaga ggtgcgaggc atcctcatct aactcgattc ctaagccgtt ttaccttcct
     1981 gaaacgagaa cttttccata ctttctcttt acaattccca tatacatcct tacaatgtgt
     2041 tctgctgttt tatttatata tttctttcta ctgaatatat tccttttcca gccagccgtt
     2101 atccacatcc aatctaacta tattttattt atgtctatcg tatccgcacc gctccccgcc
     2161 ctttccgccc tcatcatcct cgcccattac cacggcattg ccgccaatcc tgccgatata
     2221 cagcatgaat tttgtacttc cgcacagagc gatttaaatg aaacgcaatg gcttttagct
     2281 gccaaatctt tgggattgaa ggcaaaggta gtccgccagc ctgtcaaacg tttggctatg
     2341 gcgactttac ccgcattggt atggtgtgat gacggcaacc attttatttt ggctaaaaca
     2401 gacggtgggg gtgagcatgc ccaatatcta atacaggatt taactacgaa taagtctgcg
     2461 gtattgtctt ttgccgaatt ttctaacaga tattcgggca aactgatatt ggttgcttcc
     2521 cgcgcttcgg tattgggcag tttggcaaag tttgacttta cctggtttat tccggcggta
     2581 atcaaatacc gccggttgtt ttttgaagta ttggtggtgt cggtggtgtt gcagctgttt
     2641 gcgctgatta cgcctctgtt tttccaagtg gtgatggaca aggtgctggt acatcgggga
     2701 ttctctactt tggatgtggt gtcggtggct ttgttggtgg tgtcgctgtt tgagattgtg
     2761 ttgggcggtt tgcggacgta tctgtttgca catacgactt cacgtattga tgtggaattg
     2821 ggcgcgcgtt tgttccggca tctgctttcc ctgcctttat cctatttcga gcacagacga
     2881 gtgggtgata cggtggctcg ggtgcgggaa ttggagcaga ttcgcaattt cctgaccggt
     2941 caggcgctga cttcggtgtt ggatttggcg ttttcgttta tctttctggc ggtgatgtgg
     3001 tattacagct ccactctgac ttgggtggta ttggcttcgt tgcctgccta tgcgttttgg
     3061 tcggcattta tcagtccgat actgcggacg cgtctgaacg ataagttcgc gcgcaatgca
     3121 gacaaccagt cgtttttagt agaaagcatc actgcggtgg gtacggtaaa ggcgatggcg
     3181 gtggagccgc agatgacgca gcgttgggac aatcagttgg cggcttatgt ggcttcggga
     3241 tttcgggtaa cgaagttggc ggtggtcggc cagcaggggg tgcagctgat tcagaagctg
     3301 gtgacggtgg cgacgttgtg gattggcgca cggctggtaa ttgagggcaa gctgacggtg
     3361 gggcagctga ttgcgtttaa tatgctctcg ggacaggtgg cggcgcctgt tatccgtttg
     3421 gcgcagttgt ggcaggattt ccagcaggtg gggatttcgg tggcgcgttt gggggatatt
     3481 ctgaatgcgc cgaccgagaa tgcgtcttcg catttggctt tgcccgatat ccggggggag
     3541 attacgttcg aacatgtcga tttccgctat aaggcggacg gcaggctgat tttgcaggat
     3601 ttgaacctgc ggattcgggc gggggaagtg ctggggattg tgggacgttc ggggtcgggc
     3661 aaatccacac tcaccaaatt ggtgcagcgt ctgtatgtac cggagcaggg acgggtgttg
     3721 gtggacggca acgatttggc tttggccgct cctgcctggc tgcggcggca ggtcggcgtg
     3781 gtcttgcagg agaatgtgct gctcaaccgc agcatacgcg acaatatcgc gctgacggat
     3841 acgggtatgc cgctggaacg cattatcgaa gcagccaaac tggcgggcgc acacgagttt
     3901 attatggagc tgccggaagg ctacggcacc gtggtgggcg aacaaggggc cggcttgtcg
     3961 ggcggacagc ggcagcgtat tgcgattgcc cgcgcgttaa tcaccaatcc gcgcattctg
     4021 atttttgatg aagccaccag cgcgctggat tatgaaagtg aacgagcgat tatgcagaac
     4081 atgcaggcca tttgcgccaa ccggacggtg ctgattatcg cccaccgtct gtccaccgtt
     4141 aaaacggcac accggatcat tgccatggat aaaggcagga ttgtggaagc gggaacacag
     4201 caggaattgc tggcgaagcc gaacggatat taccgctatc tgtatgattt acagaacggg
     4261 tagaaggcgg gggatgggcg tgtaggctgc ttttttcgta acgttctgcg ttttcagacc
     4321 gtcgtttact gggtttactg actgaaatga aaccaaagtg caggctgccg gaaagacttt
     4381 ttctgtcttt ttaggcagcc tgcacttctt tttcactcat ctagagacct ttgcaaaatt
     4441 ccccaaaatc ccctaaattc ccaccaagac atttagggga tttctcatga gcaccttctt
     4501 ccggcaaacc gcccaagcca tgattgccaa acacatcaac cgcttcccgc tattgaagtt
     4561 ggaccaggtg attgattggc agccgatcga gcagtacctg aaccgtcaaa aaaaccgtta
     4621 cctccgagac caccgcggcc gtcccgccta cccgctgttg tccat
//
LOCUS       DAXHPG010000157         4576 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXHPG010000157 DAXHPG010000000
VERSION     DAXHPG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.157"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(25..246)
                     /locus_tag="ACFM6F_001832"
                     /pseudo
     CDS             complement(25..246)
                     /locus_tag="ACFM6F_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine catabolism dioxygenase TauD"
     gene            complement(245..352)
                     /locus_tag="ACFM6F_001833"
                     /pseudo
     CDS             complement(245..352)
                     /locus_tag="ACFM6F_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(398..1126)
                     /gene="pdxJ"
                     /locus_tag="ACFM6F_001834"
     CDS             complement(398..1126)
                     /gene="pdxJ"
                     /locus_tag="ACFM6F_001834"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC6165199.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQDKIAEFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1150..1941)
                     /gene="recO"
                     /locus_tag="ACFM6F_001835"
     CDS             complement(1150..1941)
                     /gene="recO"
                     /locus_tag="ACFM6F_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC6165200.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(1975..3102)
                     /gene="pheA"
                     /locus_tag="ACFM6F_001836"
     CDS             complement(1975..3102)
                     /gene="pheA"
                     /locus_tag="ACFM6F_001836"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC6165201.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3141..4373)
                     /locus_tag="ACFM6F_001837"
     CDS             complement(3141..4373)
                     /locus_tag="ACFM6F_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFC6165202.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
BASE COUNT         1014 a         1301 c         1269 g          992 t
ORIGIN      
        1 ctatattaca tagtcatcgc ctcaatattt aatatcccaa aatatagatt taaaaccatc
       61 gcctttgtgg tttgtcagca ttcctaattg agatagaaag tcaaataatt tcttgtttct
      121 ccggctgcca tcaagatggt ttagattaag acctcgaaca atacaatacc caatacgctg
      181 tactgtattg tgaatttctt ctatcaaact attattgttt gcagataaaa tatacttttg
      241 attcatatag tgaattaaat ttaaaccagt acggcgttgg cttgccttgc cgtactattt
      301 gtactgtctg cggcttcgcc gccttgtcct gatttaaatt taatccacta taacatatcc
      361 ctgtcgtgaa atgctgtctg aaagggtttg cctgccctta cggcagcagc cttgccctga
      421 acatcgcctc cttcatttgg cgcacggctt cgggcagtcc gaggaagagg gcttgggcaa
      481 tcagcgaatg cccgatgttc agttcgcgga tggcgaggat ttgggcgatg ggggtaacgt
      541 tgtgtatggt cagtccgtgt ccggcgttga cgaccaagcc caaatcgccg gcaaaatgcg
      601 cgccgttttg cagacgctcg aattgcttga gttgttcggc gtggctgcgc gcgtcggcat
      661 acgcgcctgt gtgcagctcg acaacgggcg cgccgacatc ataggcggct tggatttgcc
      721 tgtcgtcggc atcgataaag agagagactc ggatgcctgc gtcggtcagg attttggtga
      781 actcggcaat tttgtcttgt tgcgccaata cgtccaaacc gccttcggtc gtgatttcct
      841 gacgtttttc cggcacgatg cacacgtctt ccggcatcac tttaagcgcg ttttcgagca
      901 tttcttccgt caacgccatt tcaaggttca ggcgcgtgcg gatggcgttt ttgacggcga
      961 acacgtccgc gtctttgatg tggcggcggt cttcgcgcag gtgcatggta atcaggtcgg
     1021 caccgtgcgt ttcggcaacc agtgccgctt ccacagggct gggataagtc gtgccgcgcg
     1081 cgttgcggac ggtggcgatg tggtcgatgt tgacacctaa aagcataatc tttcctttta
     1141 tttctgcctt cagacggcat ttgaagccgt gccgtccgaa gtcgggacgg tttcccgggc
     1201 ggtttctttg cggtcaaact gccgtatctg ttccaacacc tgccgcgatt tcagcccctc
     1261 gggcaacagg tggcggataa aaagccgtgt gattttcaat gcctgttgca ggctttcggc
     1321 ggtgcggaaa ctgccttcgc gcaaatcgat caggctttgc ccttcgacaa ttgcgccggc
     1381 ttcgtgcgaa agcgcgtctg cgtcctcgca tacgggcatg accgcctctt cgggcatcag
     1441 ccggtaggtt ttgtccgcca aaatatcgcc gcccgtgccg tcggcgtgca aatcgggggc
     1501 aacgcccaag gcattgagca gcttccactc gaagcggcgc aaatcggcaa tgtggttggc
     1561 ttcacgacac accgcctcca tcacttttgc caacgcgtcg tacagttcgg gcatcggatc
     1621 ttcgcgcgcg gtcagtttca acaccagttc gttcacatac agaccgctga acaacgccct
     1681 gccctgcggc tgccgccatc cgccgatcca ttcggcacgg tgcagggttt tgagttcctg
     1741 actgccgtac cacgacgcac tgacagggac gaacggaacc aatacgccgc gcaattcgct
     1801 ctgacgtttg cgcgcgctcc gtgccagcaa agccacacgc ccgtaacggc ggctgaaggc
     1861 ttcgacccgc aggctgcttt cgcgccatgg cgaggaagcc aacaggaaga cgggttcgtg
     1921 gttgactcgg tgttcggaca tcatgcctga aatgccgtct gaacgctgct gccgctacaa
     1981 aacggcggtc gggtacgaac cgatgacttt gacgaacgaa gcgcgttcgc ccaagcgttc
     2041 caatgccgtc tgaatctgcg cgtcccggcg gtgtccttcg atgtcgatga agaacaggta
     2101 ttcccacaaa acggatttac tcggacggct ctcaaacttg gtcatggaaa tacccgactc
     2161 agtcagcggt tgcagcagcg aggcaaccgc gcctgcccgg ttgggcgcgg aaacggccag
     2221 cgaagtcttg tcgctgccgc ttgcaccggt ttcgtgatgt cccatcacca agaagcgcgt
     2281 ggtgttgttc ggttcgtctt cgatgcactc ggcaaccata tcgagtccgt agatttccgc
     2341 cgccgtgcgt ccggcgatgg cggcaaccgt accgtcgtcc gattcggcaa ccagccttgc
     2401 ggcttcggca ttgctggaca cggcaatccg ttcggcgttg ggcaggtgtc tgcccaacca
     2461 gtcgttgcac tgcgccaacg cctgcgcgtg ggaaaagact ttggcaatgc cttcggtgct
     2521 gccgttgttt ttacgcaaaa ggttgtggtg gatgcgcaaa acgacttcgc cgcacgcctg
     2581 caacgcggta acggcaagca aatccaacgt gcgcccgacc gagccttcgg tcgagttttc
     2641 cacgggcgcg accagataat ccgcctgacg ggtttcaacc tgcttgaagc agttgtctat
     2701 ggtcggacac gccatggtgt gcgcggcgtg tccgaaatgc ttgatcgccg cctgctgggt
     2761 aaacgtgccc tgcggcccca gataggcgat ggtcagcggg cgttcgacgg cgaggcactc
     2821 gctcatcact tcccgaaaca ggcgtgctac cgattcgtcg ggcagcgggc ctttgttcaa
     2881 atcctgaatg cggcgcaaca cggcgacttc gcgttcggga cggtacactg cgcccgtgcc
     2941 tttcagctcg ccgatggcgt gggcgtgttg cgcacgttcg ttgagcaggc gcaggatttc
     3001 ggcatcgatg gtgtcgatgg cgttgcggtg gggaaggagg agttcgtcga tagtttggga
     3061 cataatttcg cctgaacagt aatttatcgt gcattctaac atcaaatgcc gtctgaaacg
     3121 gtttcagacg gcattttccg ttaaaggtat tcgctttgcc cgttttcttt ccacgcacga
     3181 tgtgagcaga gccacagaag cgcaatgccg caagaggtgg acgcggtcat cgttgccgcc
     3241 atcacggttg ccgaaccgtc gtgcaagaag gtcgccgcca tacccacccc cgcgccgatt
     3301 aaagattgga atacacccaa tacggcgttt gcgctgccgc cctcttcttt gaaataagac
     3361 ataaagcatg cctgcgtgtt cgcaccgacc aagccctgcg taccgacgga aaacatcacg
     3421 cacgcgacca gcagccaaaa cgggggcaac ccgaaaaaca gcacggcggc gagttgggac
     3481 aggttggcgg caaactggac gacaatcccc cacagcagga tgctttgcgg atgcacgccg
     3541 gttttgagcc gccacgcggt aacgcggttg aaaaacatca tcgtgatgat gttgagtgca
     3601 aacgcccaag cgtattgatg aggcgtaaca cggtagagct gctggtacac gaaggaagat
     3661 tcggtcagaa aggcgaacat cgaaccgaag ctgaatgcct gaaaaaacag ataacccatc
     3721 gcggcacggg ttttcaatac gcgtttgaac cgcccagcca ccagcccgaa cacatcccgt
     3781 ccgattttgc cgccgacggc aggcttgggc aggaaatact gtaccaaacc gagcagcacc
     3841 agcgaatacg ccgccagaaa aacaaaaatc gcctgccagc cacccaagcc ctgcaacaat
     3901 gcgccgacca tgggtgcgac cagcggcaca accatcaaaa tgatgccgat aagggcaaac
     3961 atctgggcgg ctttgcgtcc ggaataataa tcgcgcacca ttgcgccgac gatgaccaca
     4021 gtcatgcccg caccgaatgc ctgcacgacg cgcaggttga ggagctgttc ggcactcgaa
     4081 gcaaatacga tggcggcaac ggcaaggcag tagacggcca gtccggtcag cgcgacgggt
     4141 ttgcgccctt tgatgtcgga caccgaaccg ccgaccacct gtccgaacgc cgtgccgaac
     4201 ataaacaaac tcaggctctg ttcgatgcgg tggacatccg cgttcagcga ctgcgccatt
     4261 tcgggaatcg cgggcaggta ggcatcgatg gaaaacggca tcagcgtaac cagcatcgcc
     4321 atcaaaaccg ccatcagttt ttcgctcatt tcaggataat gggcagaagg cataaattct
     4381 cgtccgattc gcaaacaaac cgcccggcgg cggaaaacac aatacgcgga aacggaaaat
     4441 tttaacacac cggcacaaac aggcggcaac aaaagccccg gcaaaaccgg acgaaccgga
     4501 acgatatagt ggattaacaa aaaccagtac agcgttgcct tgccttagct caaagagaac
     4561 gattctctaa ggtgct
//
LOCUS       DAXHPG010000158         4505 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXHPG010000158 DAXHPG010000000
VERSION     DAXHPG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.158"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(115..1053)
                     /locus_tag="ACFM6F_001838"
     CDS             complement(115..1053)
                     /locus_tag="ACFM6F_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6165203.1"
                     /translation="MLELNGLCKRFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAETERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRNGGIPAVLVTHSPEEACTTADEIAVMHEGKILQYGTPET
                     LVQTPADVQVARLMGLPNTDDDRHIPQHAVRFDQDGIACRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRHAGEISGNDTVHIRIEDREIVRFR"
     gene            complement(1062..1937)
                     /locus_tag="ACFM6F_001839"
     CDS             complement(1062..1937)
                     /locus_tag="ACFM6F_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6165204.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2119..2195)
                     /locus_tag="ACFM6F_001840"
     tRNA            complement(2119..2195)
                     /locus_tag="ACFM6F_001840"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2159..2161),aa:Met,seq:cat)
     regulatory      2345..2444
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2535..4436
                     /gene="thiC"
                     /locus_tag="ACFM6F_001841"
     CDS             2535..4436
                     /gene="thiC"
                     /locus_tag="ACFM6F_001841"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC6165205.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRG
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGGNVTQLHYARRGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTHPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1074 a         1299 c         1200 g          932 t
ORIGIN      
        1 gcggtttcag ttgtttccga taaatttttg cagccctgat aaaaaatatg gctgctttgg
       61 taaaaaaatg ccgtctgaaa ggtttttcag acggcatttg tttttaagga agcatcagcg
      121 gaagcggacg atttcccggt cttcgatacg gatgtgtacc gtatcgtttc ccgatatttc
      181 accggcgtgc cgcatatcga ggttcagcca caggatgccg tgttccggat ggaggacgga
      241 caggctgaac gattcgggca aacaggtgcg ggacaatacg cggcacgcta tgccgtcttg
      301 atcgaaacgc actgcatgtt gcggtatatg gcggtcatcg tcggtgttgg gcaaccccat
      361 cagtcgggcg acctgcacgt cggcaggcgt ttgaaccaag gtttcgggcg tgccgtattg
      421 gaggattttc ccttcgtgca tcacggcgat ttcgtccgcc gtcgtacagg cttcttcggg
      481 cgaatgggta accaaaacgg cagggatgcc gccgtttcgg atgcgttcgg cggtcatacg
      541 gcgcagcgtg ccgcgcaaat gcgtgtccaa actggaaaac gattcgtcca gcaacagtag
      601 ggaagggcgg acaaccaaag cgcgtgccag tgccaaccgt tgtttctcgc ctccggaaag
      661 tttttcaggc ttgcggtgtg cctcgttttc cagtccgact tcggcaagtg ccgccatagc
      721 aaggcgttcg gtttcggctt tcggcatttt ttgcattttc aaaccgaatg ccgcattttc
      781 cagcgcactc atatggggaa acagcgcgta atcttgaaac atcagcgaaa tacggcgttt
      841 ttcgggcggc atacgggtaa tgttttcccc gttcagctga atttcgccgc cgtccggccg
      901 gacgatgccc gcaatcatat tcagcagggt ggattttccg cagcccgacc gccccagtac
      961 ggcgagtatt ttgccgcgcc cgacagtcag gcagatgttg tcggcaaccg ttttgccgcc
     1021 gaagcgtttg cagagtccgt tcagttcaag catggcgcat cctataaacg tatgccgtcc
     1081 agccactcgg acagcggatg ggcggcatca atcagaccgt aacggcaaaa cgcgtccagc
     1141 gtaaccagtt gcgcgtcgtg catcatgttt cccgacaaca tggtggcaac caagctgccg
     1201 atgtccattt tttcaaaaca cgcaacctcg ccgtcctgat tttcaggcag gaaggtttcg
     1261 ggtaggacgg catcgaatac atacaggatt tcattgtgca caccccggct gacggggcgc
     1321 aggctgtgca gccgggatac cggacggata aacggaaaca gcgttttatc caaaccggct
     1381 tcttcgctgc tttcgcggca cacggcttca gacggcattt cgccgccgga aacaccgccg
     1441 gcggcaatat tgtcgagttt gtcgggatcg actgctttgt gcggactgcg cttgcctatc
     1501 cagaaatgcc atcggccgtc cgattcggtc agtccgttga gatggacggc gcggctgagc
     1561 agtccgaacg gacggaaagc ggcgcgttcg agcgtgaaca aggggttgcc gccgccgtcg
     1621 gtcaggtcga aacactcgtt gcgccatccg tgaagcagcc ccgccttgtt ccatgtgcgg
     1681 gcgaggtgct gcaagcggcc gcccatatct gaccagccgt ccgcattcag gaaaatgccg
     1741 tctgaagact ccgagcagcc tgcctcccag tcttttttga cgcgctccgc ccattccggc
     1801 gacagattgc ccaaaggcag acggttcaga tacagcgttt tccagcaact ttctgcaccg
     1861 taacttgctt ttgcccactc gaacagggca tcaaggtcgt gtttgctgac ggattcggta
     1921 aaacggacgg tcggcatagt cggctttctt gaagacaatg cggaagattg tagccaaact
     1981 gcctgaattt gcaaccgcaa acagacggcg ggctgccgtt ccgatattgc ggcaggaagg
     2041 aaaatccggc aaaaaacgga ggcggcagga aacaggtaaa acaaaacccc tcggcattga
     2101 accgaggggt ttgatatttg gttgcgggag caggattcga acctacgacc ttcgggttat
     2161 gagcccgacg agctaccatg ctgctccatc ccgcgtcaga agatgaaact atacggcaga
     2221 ttttttatgt tgtcaaacat tacttccgcg aaaaatcata aaattttgcc gtccggcggt
     2281 ttatgcctga tttgaaatat tattttcttt acaaaagttc atgttcgtga tttaattttg
     2341 gttaacattg aaacaggggt gctgcctgat gttcaggcgg ctgagaaata ccctttacac
     2401 ccgatcggga taatacctgc gtggggagtt ttcacggatt ctgcctttca gacagcattg
     2461 gttttcaaat gccgtctgaa aacgcgaaac gctcctgttt ctttatttta aacgagaaaa
     2521 caggagcatt ttttatgact acgccaaaaa aaaccgccaa aacttccggc aacgaagcgc
     2581 gcgaacttgc cgacttgagc gaagacatcg gcatccgctt caaatatccg aactcggaac
     2641 gcgtgtatct gcaaggcagc cgtggcgaca tccgcgtgcc tttgcgcgaa atccgccagg
     2701 acgacaccta cacggcgcaa ggcacggaag ccaacccgcc gattcccgtc tatgacacca
     2761 gcggcgtgta tggcgatccg gcggcgcaca tcgacctgaa acaaggcctg ccgcacatcc
     2821 gcaccgcatg gctgaacgaa cgcggcgata ccgaaatcct gcccaagctc tccagcgaat
     2881 acggcatcga acgcgcacac gatccgaaaa ccgcccatct gcgcttcaac caaatcaccc
     2941 gcccgcgccg cgcgaaaagc ggcggcaacg ttacccagct tcactacgcg cgccgcggca
     3001 tcatcacgcc cgaaatggag tttgtcgcca tacgcgaacg tttaaaatta gacgaattgt
     3061 cccaaaaacc ggaatacgcc aaactcttga aacagcacgc agggcaaagt ttcggcgcga
     3121 atatcccgac ccatcccgac caaatcacgc ctgaattcgt gcgccaagaa atcgccgccg
     3181 gacgcgcgat tatccccgcc aacatcaacc accccgaact cgaaccgatg attatcggcc
     3241 gcaacttccg cgtcaaaatc aacggcaatt tgggcaactc cgccgtcacc tccagcctga
     3301 ccgaagaagt cgaaaaaatg gtgtggtcgc tgcgttgggg cgcggacaca attatggact
     3361 tatccacagg cgcgcacatc cacgaaacgc gcgaatggat tatccgcaac gcgcccgtcc
     3421 ccatcggcac tgtgccgatt taccaagcgt tggaaaaaac cggcggcatc gccgaagatt
     3481 tgacttggga tttgttccgc gacaccttaa tcgaacaggc ggagcaaggc gtggactatt
     3541 tcaccataca cgcgggcgtg ttgctgcgtt atgtaccgat gaccgccaac cgcctcaccg
     3601 gcatcgtctc gcgcggcggt tcgattatgg cgaaatggtg tttggcacat catcgggaaa
     3661 atttcctcta cacgcatttc gacgaaatct gcgaaattat gaaagcgtat gacgtatcgt
     3721 tcagcctcgg cgacggcctg cgccccggct gcattgccga tgccaacgac gaatcccaat
     3781 tcgccgaact gcacaccttg ggcgaattga ccgataaagc gtggaaacat gacgtacaag
     3841 tcatgatcga aggccccggc catgtgccgc tgcaacgcgt caaagaaaat atgaccgaag
     3901 agctgcaaca ctgttttgaa gcgccgtttt acaccctcgg cccgctcgtt accgacatcg
     3961 cccccggcta cgaccacatc acctcgggca taggcgctgc caatatcggc tggtacggca
     4021 cggcgatgct ctgttacgtt acccccaaag aacatttggg actgcccgac aaagaagacg
     4081 tgcgtaccgg catcatcacc tacaaactcg ccgcccacgc cgccgacctc gccaaaggct
     4141 ggccgggcgc acagttgcgc gacaacgccc tgagcaaggc gcgtttcgag ttccgctggc
     4201 gcgaccagtt ccgcctaagc ctcgaccccg aacgcgccga aagcttccac gacgaaaccc
     4261 tgcccgccga aggcgcgaaa atcgcccact tctgttcgat gtgcggcccc aaattctgct
     4321 cgatgaaaat cacgcaggaa gtgcgcgact acgccgacaa gcaaaaagcc caacggcagg
     4381 gcatggagga aaaagcggtc gagttcgtca aaaaaggcgc gaagatttac agttgaaacg
     4441 tcaagcaaaa aatgccgtct gaaacccgga aaaaaggctt cagacggcat tctttcgctt
     4501 gtgaa
//
LOCUS       DAXHPG010000159         4444 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXHPG010000159 DAXHPG010000000
VERSION     DAXHPG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.159"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(63..1205)
                     /locus_tag="ACFM6F_001842"
     CDS             complement(63..1205)
                     /locus_tag="ACFM6F_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC6165206.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQTALANARSA
                     GVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKYYAG
                     WLAAMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1254..1676)
                     /locus_tag="ACFM6F_001843"
     CDS             complement(1254..1676)
                     /locus_tag="ACFM6F_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165207.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPVGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1669..2478)
                     /locus_tag="ACFM6F_001844"
     CDS             complement(1669..2478)
                     /locus_tag="ACFM6F_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6165208.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEYAGSVWMMVQTREQWRQAQEKGADVVVWRVGNAAQ
                     AAAVDEVLKHGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2560..2937)
                     /gene="acpS"
                     /locus_tag="ACFM6F_001845"
     CDS             complement(2560..2937)
                     /gene="acpS"
                     /locus_tag="ACFM6F_001845"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC6165209.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3137..3904)
                     /locus_tag="ACFM6F_001846"
     CDS             complement(3137..3904)
                     /locus_tag="ACFM6F_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6165210.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3914..4444)
                     /locus_tag="ACFM6F_001847"
                     /pseudo
     CDS             complement(3914..4444)
                     /locus_tag="ACFM6F_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
BASE COUNT         1101 a         1108 c         1080 g         1155 t
ORIGIN      
        1 tcggccggat attaaaaaaa gccgtctgaa catttttcag acggcatttt tgtacacttt
       61 cctcaacggt tcgatccctg caccatatca atcaggaaca ggcggcaatc gaggttgccg
      121 ttataaagcg gaattttgcg ttttggcgac aggcgcatga atttgggcat ttcccgatct
      181 ccggtaaaca ttgccgccaa ccagcctgcg taatatttct tcaaccacgt ccccaactgc
      241 ggataaagcg cctgcaaggc gcggacttcc tcaaggcgca cgccgtaggg cggattggac
      301 accataatgc cgttttcgcc gttcggacgg acggattgcg cgtcggcaac gctgaaggaa
      361 acgatgtcgt ccaccccggc actgcgtgcg ttggccaatg ccgtctgaat gatgcggcgg
      421 tcgctgtcgc tgcccgaaat cggtgcaaaa gccggtttaa cctgcgcttc ggcacggcgg
      481 cgcaaatccg accacagcgt tttatcgaaa ttttgcagtt tttcaaaacc gaaacggcgc
      541 atcatacccg gcgcgcggcg ggcggcaatc caagcggctt cgatagcaat cgtgccgctg
      601 ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc ctgccgagag cagcaatccg
      661 gcggcaaggt tttcgcgcag cggggcttcg ccggtatcga gacggtagcc gcgtttgaac
      721 agggcttcgc ccgaagtgtc gatgaagatt tcgacattgc gttcgtccaa aaaggcgtgg
      781 atgcggacat cgggcgcggc tttgtccacg ctcggacgtg cgtcgtaaat gtcgcggaaa
      841 gcgtcgcaga cggcatcttt gacggtcagt ccgacaaatt ggatgctctt aacgttggca
      901 cgctttgcct cgactttgac tttgaacgtc tgctgtaaag taaaccaatt aaaccagttg
      961 atatttttgg cgagtttgta gatgtcgcgc tcattgcggt atgtcccttt ggtcaggcgc
     1021 agcaggatac ggctggcagt acgcgaatgc aggttggcgg cgtaaacctg ttccaatccg
     1081 ccgcggcagg aaacgccgcc gtcaaacgct tgaacatcgg tacagccgag gcttccgagt
     1141 tcttgagata aaacggtttc caagccgcgc gggcaggtgg cgaaaagtgt atataaagta
     1201 ttcataacgt gtcctttccg gacggatgtt ccggcaatgg cggattgaaa ggtttattcg
     1261 gcggtaaatg ccgtctgaaa tgtccggctg ccgattgtaa tgtccgccgt aaaatcgcgc
     1321 ctgccttcga cacagatggg caggcggatg cgtactgcct gccaagtccc cgacggttgc
     1381 cgctcgaaca tatagcggtt gaaacccata tccatatttt tcatactgaa gctgatgctg
     1441 acctgttccg tgccgacggg cgcgtgttcg atataaatat caaacggttt tttggttgaa
     1501 acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc aaccctcggt caaatcgcat
     1561 tgcgccgcca cggcttgcgg ctgctgcgcc tgccaccatt gcaacaaaac gagcttgacg
     1621 gcggcaaagg caatcagcag cagtgcggca agcagtaatt tacggttttt attcatgatt
     1681 cttccctatt gtttcatccc ttaccaccac gtgcgccccc aatccgagcc atagttttcc
     1741 ataacgtgca accgtctgtc cgtttgctgc aagtacgagc ggcacggata cgccgtgctt
     1801 caacacttcg tctaccgctg cggcttgtgc ggcattaccg actcgccaga cgaccacatc
     1861 cgcgcccttt tcctgcgcct gccgccattg ttcgcgggtc tgcaccatca tccacacgct
     1921 gccggcgtat tcgggtctgt cctgccattg cgccgcctcc atcaaaacgt tcgcaccgct
     1981 tccctctgcc gaacccaaag gcaggacgcg gtacgcgccc atactgtttt ctccgtgcaa
     2041 acccgttttc aggctgccgt acaaacggcg cgggacggac agcgaacgca aaagcgcgcc
     2101 gttggcgggc agcatggggg caacggtaaa atcacccgcc ttctgccaag accattcctg
     2161 cccttcgcgg gattgcggtt cgcccgtcca ttggtcgggg ttgacccata ggaatttcag
     2221 gcagacgcgg gcgtgttcgt aggaatggat tttggtcagc caaggcgtgg cggcgaggat
     2281 gcggatgccg agttcttctt caaactcgcg ttgcaaggct tggaagtcgg tttcgcccgc
     2341 ttcgaccttg ccgccggcaa attcccaata tccggcatag ggtttgcctt cggggcgcga
     2401 gctgagcagg tagttgccgt ctgaatcgag caggatgccg gcaacgacgc ggataagggg
     2461 tcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt cggagtgcaa atgagggacg
     2521 ggcaatgccg tcaacgggtt tgccgcattt cagacggcat tatttttcgg caacgacaaa
     2581 cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg acgcggctga tgccttgttc
     2641 ctccagccat ttggacaggg cggggccgta gaaaaattcg ggcttgccca atgcgtcatg
     2701 cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg cccgtgccga cggctttggc
     2761 aaaggcttct ttggcggcaa agcgtttggc gaggtagttg acgggtttgc ccgcttgcgg
     2821 aaattcaagc agctcttccg gagtgaggat gcgcccggca aacgcctgtc cgaatttttt
     2881 gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg ccgatgccgt aaatcatatt
     2941 tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg atgatgaatg ccgtcaattt
     3001 cttggggcgt taaaatggcg tgaatatcca cttttggggt tttgtcggta acaattttca
     3061 ctgtaacctt ttgcatttga aattctaaaa catcgtgcca attatttaat aaggagttaa
     3121 ggcatttaat aatgaattaa agtttgatta aacccgaatg cccgattttc agagccaata
     3181 ccaataaaac aataaaaatt aacattttaa aaaaatttgg gctaatctta gtccttaacc
     3241 gaattccaac atacaatcca ataacagaca atacggacag taaaaatatt aaaccgtatt
     3301 cactcttatt taataaccaa tactggtctc ttagcatata tatttgaaca attttcgcca
     3361 aaagatagca tagattgctt gattttacga tacgattttt attttctgtt tcgctaagca
     3421 aaaatattaa caatatggga gacatggcat tggttgaacc gccgatgatg cctgccaaaa
     3481 acccaaaaag aaccatattc ttattattgg caactacttg aatatttttt gcttttgcac
     3541 atacatttaa aataccattg acagaataat acaatgtaat gattgccatc agtaaaagca
     3601 gccaagacac tggaagtatc aaaagcaact tcacccccaa aatgctgcca acgacgctgc
     3661 cgatagcaag caatttatag gtttttaaat aataaacaat ctcttgccaa aaaccctttt
     3721 tgttattgct gcatagaacc aacaagctca ttaacaggct tggtaatgcc accaaggcaa
     3781 caaccttaga caatggcatg ataaaagcca atgcggttgt accgagcatc ggaaatccca
     3841 tgcctgtaat tccgtgcagt attgcggcag caacaaaaac gatagattgc attatttctt
     3901 gcataaccgt cctttataca gtaattcttg ctctaatata atgcctgttt ttatccgtat
     3961 aatcagttct ggcatgcaag ccattgacat tgtcaataat aatcaaacca tcatcttgtg
     4021 cagaaaattc ttgaatatca ctcgcatttt ttataacatt tacaagataa tcaagtgcaa
     4081 gtaccatttc ctttgatact ttatttctat tttcttcaat accttataga tacaatcact
     4141 tctaaatcta atcatttgag tattgaccga taagatatta ccccatctca caccttgttt
     4201 tttatcaaat gatgctggtg ttttaaatgg atataaattg cccgttaatg tttttaagtg
     4261 ttttttaccg gtttctgttt tagataactg attgacaata tctgatgaac ttaaaaatag
     4321 ggaattacct ccatcattgg ctgattttac tacatacata accaaataac tttccgggtt
     4381 ttcactaaaa gatgaatcac tatgaagtgg acattctcca acatcttccg agaaagttat
     4441 atca
//
LOCUS       DAXHPG010000160         4373 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXHPG010000160 DAXHPG010000000
VERSION     DAXHPG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.160"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(69..2045)
                     /locus_tag="ACFM6F_001848"
     CDS             complement(69..2045)
                     /locus_tag="ACFM6F_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC6165211.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="ACFM6F_001849"
     CDS             2194..3033
                     /locus_tag="ACFM6F_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC6165212.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3234..4265
                     /gene="gap"
                     /locus_tag="ACFM6F_001850"
     CDS             3234..4265
                     /gene="gap"
                     /locus_tag="ACFM6F_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6165213.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKDKLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1118 a         1466 c         1002 g          787 t
ORIGIN      
        1 tgaattttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggcctgtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccatttt cgccgaatcc tgcctcatca tcactaccga caaacaaacc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccggta cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg tcagcaccac cattttcgcc cgccccaaac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagcaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccttgtgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatcc ccgccacaaa cgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaatac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccaatgc tgaacatcag gaacacaatc
     1861 ccgatttcac ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg tgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgtgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaaaac
     3061 cgtttcagac ggcattttat caagctctcc gtccgccgca cccgtgccgt ccgcatctta
     3121 ccgcccaccc ttccggccgc gccgttttca ataaaatatt aattacacgc cactacaaat
     3181 ttgctataat ccgccccgaa aatctaccca acccctcaac aaaggaacaa accatgggca
     3241 tcaaagtcgc catcaacggc tacggacgca tcggccgcca ggttttgcgc gccatctacg
     3301 attatcagat tcaagaccaa ctccaaatcg tcgccgtcaa cgccagcggc agccttgaaa
     3361 ccaacgccca cctcaccaaa ttcgacaccg tgcacggacg ctttgaagcc gacgtatccc
     3421 acgacggcgg caacctcatc gtcaacggcg acaaaatccc cttcttctcg acccgcaacc
     3481 ctgccgaact gccgtggaaa gaactcggtg tcgatttggt catggaatgc accggcgcgt
     3541 tcaccagcaa agaaaaagcc aaagttcatc tggaaagcgg cgcgaaaaaa gtcctcattt
     3601 ccgcaccggg cggcgacgat gtcgatgcaa ccgtcgtgta cggcgtgaac gacagcatcc
     3661 tgaccgccga catgaccgtc gtttccaacg cttcctgcac caccaactgc ctctcgccgg
     3721 ttgccaaagt gttgagcgaa agcgtcggca tcgtcaaagg cgcgatgacc accatccacg
     3781 cgctgaccaa cgaccaaacc gttaccgacg tgcgccacaa agacctgcgc cgcgcccgca
     3841 gcggcgtgga aaacatgatt ccgaccaaaa ctggcgcggc aaaagccgtc ggactggtac
     3901 tgcccgaatt gaaagacaaa ctcgacgggc ttgccatccg cgtgccgacc gtcaacgtat
     3961 cattggtaga tttgaacttc caagccgcgc gcgacacgac aatcgaagaa atcaacgcac
     4021 tgatgaaagc cgcctcggaa gcaggcgcgc tcaaaggcgt tttaggctac aacaccctgc
     4081 ccttggtttc catggacttc aaccacacca ccgaagccag ccacttcgac gcaacactga
     4141 ccaaagtcgt tgacggcaac atgattaaag tcttcgcatg gtatgacaac gaatggggct
     4201 tcagctgcca aatgctgaac accgcacgcc gtatgttcgg acttgaagtg cgcccgctca
     4261 aataagcaat aaaccgtcaa acaaaatgcc gtctgaaacc cgatgttttc aagttccaga
     4321 cggcattttt catttttcac cgtgatttta cccggctgtc gtcatttcta att
//
LOCUS       DAXHPG010000161         4368 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXHPG010000161 DAXHPG010000000
VERSION     DAXHPG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.161"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(10..576)
                     /locus_tag="ACFM6F_001851"
     CDS             complement(10..576)
                     /locus_tag="ACFM6F_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HFC6165214.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGS
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIQ"
     gene            complement(1228..1449)
                     /locus_tag="ACFM6F_001852"
     CDS             complement(1228..1449)
                     /locus_tag="ACFM6F_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165215.1"
                     /translation="MPSETSFDRFRRHFPIPPPPFQWAFFIKRKISRHSRPTAHFSAV
                     IPAPSPPVQTGTKTFPRHSRESGNLERKI"
     gene            complement(1525..3039)
                     /gene="katA"
                     /locus_tag="ACFM6F_001853"
     CDS             complement(1525..3039)
                     /gene="katA"
                     /locus_tag="ACFM6F_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC6165216.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            3387..4238
                     /locus_tag="ACFM6F_001854"
     CDS             3387..4238
                     /locus_tag="ACFM6F_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6165217.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDARFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALQKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESLEIPAA
                     HKRKTAE"
BASE COUNT         1000 a         1164 c         1164 g         1040 t
ORIGIN      
        1 tttccgacat cattgaatca aacccaaatg cgacaagagc gtccatgtac cgatggcaat
       61 caacaccaaa cctccggcaa attccgcacg cctgccgaac aatacgccca aagcccttcc
      121 cgccgtcagc ccgaccgcca ccatcaccgt cgtcgccata ccgatgattg cggcggcaaa
      181 ggcgatgttt acctccataa acgccaagcc caccccgact atcatggaat caatactggt
      241 tccaaaagca gtcaaaaccg tcatccatag gctttcccgt ttgctttcgc gcacatcttc
      301 cgccttgccg gacagccctt cgcgcatcat tttcagaccc agcccgccca gcaggacgaa
      361 agccgcccaa tggtcccatt cgctgataaa cggtttggca taaaaaccgc ctacccagcc
      421 tgccagcggc gtgagtgctt caaccgagcc gaacaccaag gctgttgctg caattttgcg
      481 cggaggcatc ctgaccgccg caccctttgc caatgcgacg gcaaacgcat ccatcgacat
      541 ccccagcgca atcaagagca aagcgtaaag actcataccg cacccatcct caaaaagggc
      601 agattatagc aaaacagcaa agcgcgaaaa acgccgcacg aaaacccaca tcccgtcatt
      661 cccgcgcagg cgggaatcca gagttgtcgg tgcggaaact tatcgggaaa aacggtttct
      721 ttagatttta tagtggatta acaaaaacca gtacagcgtt gcctcgcctt ggctcaaaga
      781 gaacgattct ctaaggtgct gaagcaccga gtgaatcggt tccgtactat ctgtactgtc
      841 ttcggcttcg tcgccttgtc ctgatttttg ttaatccatt atatgaatcg tcattcccgc
      901 gcaggcggga atccagagtt gtcggtgcgg aaacttattg gataaaacgg tttctttaga
      961 ttttacgttc tggattccca ctttcgtggg aatgacggga ttttggtttc cgtgcggatg
     1021 gattcgtcat tcccgcgcag gcgggaatcc aaagttgtcg gtgcagaaac ttatcgggaa
     1081 aaacggtttc tttagatttt acgttctgga ttcccacttt cgtgggaatg acgagatttt
     1141 ggtttccgtg cggatggatt cgtcattccc gcgcaggcgg gaatccagag ttgtcggtgc
     1201 ggaaacttat cgggaaaaac ggtttcttta gattttacgt tctagattcc cgctttcgcg
     1261 ggaatgacgc ggaaaagttt tcgtgccggt ttgaaccggt ggggatggtg cgggaatgac
     1321 ggcggaaaag tgggctgtgg ggcgggaatg acgggatatt ttgcgtttaa taaaaaacgc
     1381 ccactgaaac ggcgggggcg ggatggggaa atgccgtctg aagcggtcaa acgacgtttc
     1441 agacggcatt tttatgcccg gttatttccg gtagcggacg gcgcgggaca ggatttcttc
     1501 aatttccatc cacataatgc ccccttacag caaaccgggc tggccgagtg cggggtcggt
     1561 cgcgcgggcg gcttgtgcgt cttcgacagt cagtccgagg gcttttgcca cgccttcgcc
     1621 gtatgccgga tcgcaacggt agcagttgcg gatatggcgg tatttgatga agtcgggcgc
     1681 gtcgcccatt gcggcggcgg tgttgccgaa caatgcctgt ttctgcgcgt cgttcatcag
     1741 gttgaacagg gcgcgcggtt ggctgaaata gtcgtcatcg tcttggcggt agtcccagtg
     1801 cgccgcgtcg ccgttgattt tcaaaggcgg ttcggcgaag tcgggttgtt gctgccattg
     1861 gccgaagcta ttgggttcgt agtgcggcag gctgccgtag ttgccgtcgg cgcgtccttg
     1921 tccgtcgcgc tggttgctgt gaacagggca acgcggacgg ttgacaggga tttggcggaa
     1981 gttcacgccc aagcggtagc gttgcgcgtc ggcgtaattg aacaaacgcg cttgcagcat
     2041 tttatctggg ctggcgccga caccgggaac gaggttgctc ggtgcgaagg cggattgttc
     2101 cacatcggcg aagaagtttt cgggattgcg gttcaactcg aattcgccca cttcaatcag
     2161 cggatagtct tttttcggcc aaactttggt caagtcaaac ggatgatacg gcactttttc
     2221 cgcatcggct tcaggcatga cttggatgta catcgtccat ttcggaaact cgccgcgctc
     2281 gatggcttcg tacaggtcgc gctgatggct ttcacggtcg tcggcgatga tttttgcagc
     2341 ttcttcgttg gtcaggtttt taatgccttg ttggctgcgg aaatggaatt tcacccaaaa
     2401 acgctcgccc gcttcgttcc agaagctgta agtatgcgaa ccgaagccgt gcatatggcg
     2461 gtagccggct gggatgccgc ggtcgctcat cacgatggta acttggtgca gggcttcggg
     2521 cagcagcgtc cagaagtccc agttgtttgt ggcagagcgc atattggtgc gcggatcgcg
     2581 tttgacggct ttattcaggt cggggaactt acgcgggtcg cgcaggaaga acacgggcgt
     2641 gttgttgccg accacatccc agttgccttc ttcggtataa aatttcaatg caaaaccgcg
     2701 gatgtcgcgt tctgcatcgg ctgcgccgcg ttcgcctgcc acggtggtga aacgggcgaa
     2761 catttcagtt tttttgccga cttcgctgaa gattttggcg cgggtgtatt tggtgatgtc
     2821 gtgtgttacg gtaaacgtac cgaacgcgcc cgaacctttg gcgtgcatac ggcgttcggg
     2881 aatgacttcg cgcacgaagt cggcgagttt ttcattcagc cacaaatcct gcgccagcag
     2941 agggccgcga ggaccggcgg tcaggctgtt ttgattgtcg gcaacaggcg cgccgttgtt
     3001 catggtcaga tgggttacag ggcatttgga ggtagtcatc gctcttgttc cttttctcag
     3061 gttggtcaaa tgggggtaaa cggcttacag tacgatttgg cggaaagcgt attcgtaacc
     3121 ggtttcttga ttgcaataaa tttcttgaat cgacatttta tttccctttt gtaaaaacta
     3181 tggatgcgac tatacgccaa gattttcgct attaaaacta tgaaatcgat ttaatattat
     3241 tataagcaat cggttcttga ttttcgtttg ttttttgtta tcgaacggaa tccgaacccg
     3301 ctcattaaaa ccatttataa tgcaatgacg ctttgcggca ttttttgcgc cgacaggctg
     3361 aaaataacaa ttttccccac attatcatga ccttactcgg aataaagctc aaacagaccc
     3421 agcagctcaa ccagcggctg caacaatctt tgcgcgtatt gcagatgtcg ggtatcgaac
     3481 ttgaacgcga ggtcgaaaac tggctgtcgg acaaccccct gctcgaacgc aaagacacgg
     3541 atgaattttc cgatgctagg ttttcccatt acaccgcgcc tgcccgtcaa atcggcggag
     3601 acgaaggcga agatatgctg tccaacatcg ccggtgagca ggatttcaag caatacctgc
     3661 acgcgcaagt atgcgaacac ccgctttccg accaagaagc cgcctgtgtc cacatcctta
     3721 tcgatttcct tgacgagcag ggttatctga ccgacagcat cgaagacatc ctcgaccata
     3781 cgcccttgga gtggatgttg gatgaagcaa tgctgaaaca agccctgact gcattgcaaa
     3841 aattcgaccc ggcaggcgtg gccgccgccg atttgaacga atcgctgata ctgcagatag
     3901 aaagattggg cgaatgtgct gccaaacccg ccgccctgca tatcgtccga aacgccctcg
     3961 acagcattga cggcaaccgc agccaaaccc tcgcacgaat aaaaaaacac ctgccccaaa
     4021 ccgacagcgg cacactcgaa gccgcactcg acctcattgc ttcgctcaat ccctttcccg
     4081 ccgccggttt tgcctcgtcc acacccacgc cgtattctga cgaggcactc gccaacctgc
     4141 tggctttccg cggcatggag gtttctcgcc gcaccattgc caaatacaga gaatcccttg
     4201 agattccggc agcacacaaa cgcaaaaccg cagaataatt gctgaataat cttataaaga
     4261 caacaaacca aaagccggca tttctgcgaa agcgggaatg ccgaatccgt ccgcgcggaa
     4321 acctgcatcc cgtcattccc gcgaaagagg gaatctagaa acgcaaag
//
LOCUS       DAXHPG010000162         4309 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXHPG010000162 DAXHPG010000000
VERSION     DAXHPG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.162"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(153..2081)
                     /gene="dnaK"
                     /locus_tag="ACFM6F_001855"
     CDS             complement(153..2081)
                     /gene="dnaK"
                     /locus_tag="ACFM6F_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC6165218.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLITRSIEPCKIALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVRDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2289..2561)
                     /locus_tag="ACFM6F_001856"
     CDS             complement(2289..2561)
                     /locus_tag="ACFM6F_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165219.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2747..3433
                     /locus_tag="ACFM6F_001857"
     CDS             2747..3433
                     /locus_tag="ACFM6F_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6165220.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3565..3903
                     /gene="erpA"
                     /locus_tag="ACFM6F_001858"
     CDS             3565..3903
                     /gene="erpA"
                     /locus_tag="ACFM6F_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC6165221.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3920..4066
                     /locus_tag="ACFM6F_001859"
     CDS             3920..4066
                     /locus_tag="ACFM6F_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165222.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4063..>4309
                     /locus_tag="ACFM6F_001860"
     CDS             4063..>4309
                     /locus_tag="ACFM6F_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165223.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITR"
BASE COUNT          976 a          857 c         1148 g         1328 t
ORIGIN      
        1 aaaaaatttc cagagaaccg atataacagt tagaacttgg gcttgggaat atcacggtag
       61 atgaatttgg aacctctgtt atgctatggt cttttatctc aattgaaaaa agcgcgaatc
      121 aaacggttcg cgcttttttc agacggcatc aattattttt tgtcgtcttt tacttcttca
      181 aagtcggcat ctacgacatc atcgtctttc tttgcagaag cattggcttg ttcgctttcg
      241 cctgcttggg cttcagcttg tgcttgagcg taaaccattt cccccagttt ttggctggct
      301 gtgcccagtg cttcggtttt ggcatcgata gcggctttgt cgtcgccttt aactgcttct
      361 tcggcttctt tcagcgcggc ttcgattttt tctttctcgg ctgcgtcgag tttgtcgccg
      421 tagtcggcca aagatttttt cacagagtga atcagggctt cggcttggtt gcgggaagcg
      481 accaattcag tcagtttttt atcttcctcg gcattggctt cggcatcttt caccatgcgt
      541 tcgatttctt cttcgctcaa acctgaagaa ccttggatgg tgatgttggc tgctttaccg
      601 gtgcctttgt ctttggcgga aacgtgcagg atgccgttgg cgtcgatgtc gaaggttact
      661 tcgatttgcg gcataccgcg cggtgcaggt gcgatgtcgc ccaagttgaa ctgacccaaa
      721 gatttgttgg cagaagcgcg ttcgcgttcg ccttgcagta cgtggatggt tactgcgctt
      781 tggttgtctt cggcggtaga gaacacttgc gatgctttgg tcgggatggt ggtgttcttc
      841 tgaatcagtt tggtcatcac gccgcccatg gtttcgatac ccaaagacag aggagttacg
      901 tccagtagca atacgtcgct gcggccgccg ctcaatactt cgccttggat cgctgcgcct
      961 acggcaacgg cttcgtcagg gttcacgtct ttgcgcggtt ctttgccgaa gaagtctctc
     1021 acggcttctt gtactttcgg catacgggac tgcccgccga ccaagattac gtcgtcgatg
     1081 tcgccggtgc tcaagccggc atctttcaat gcaattttgc aaggttcgat agagcgggta
     1141 atcaggtctt caaccaggct ttcgaatttg gcgcgggtaa ttttcatcgc caagtgtttc
     1201 gggccggttg cgtccatggt gatgtacggc aggttaattt cggtttgctg gccgctggac
     1261 aattcgattt tggctttttc ggcagcttct ttcaggcgtt gtagagccat cacgtcttgt
     1321 ttcaaatcaa tgccttgttc ttttttgaac tcggcgatga tgtggtcgat gaggcgttgg
     1381 tcgaagtctt caccgcccaa gaaggtatcg ccgttggttg ccaatacttc gaattgtttg
     1441 tcgccgtcga ggttggcgat ttcgatgatg gaaatatcga aagtaccgcc gcccaagtca
     1501 tatacggcta ctttgcggtc tttgttgtcg cctttgtcca taccgaatgc caaagcggct
     1561 gcggtcggct cgttgatgat gcgtttcacg tccaaaccgg cgatacggcc tgcgtctttg
     1621 gtggcttgac gttggctgtc gttgaagtag gcagggacgg taatcacggc ttcggttact
     1681 ttttcgccca agtaagcttc ggcggcttct ttcattttac gcaggacttc tgcggaaatt
     1741 tgaggaggag acagctcttt gccttgtgct tttacccatg cgtcgccgtt gttggctttg
     1801 atgatttcga aaggcataga ttcgatgtcg cgttggactt ctttgtcttc aaatttgtgg
     1861 ccgatcaggc gtttggcggc gtaaatggtg tttttagcgt tggttacggc ttggcgtttg
     1921 gcaggcgcac cgacgaggat ttcgccgccg tccaaataag cgataacgga cggcgtggtg
     1981 cgtgcgcctt ctgcgttttc gatcactttg gtttgaccgt tttcggaaat ggccaaacaa
     2041 gagttggttg tacctaagtc gataccgatt acttttgcca tgtggataat cctatttgat
     2101 tttgcttatt ttgagaaata tgttggaaca ttttgtcccg atgggctgta aatagggcgg
     2161 gcggcgggct gtttcaagct acagcatggc tataagtata taactttatg aatatattgg
     2221 ttttatattt gatttaatac atttggctcc aatgcattta agcataatat ttcaaatggc
     2281 aggcaggttt attcatagac gatgccggcg agcatttcct gttcgttcaa gttgccgtac
     2341 tctttttccc agtcgtgtga agactcgatg atgtcgcatt ctttggaaag ggagacttgt
     2401 tctgcatcca tatctttggc gttcagtatg ttgaattgtt cgcacaggga tgcggataaa
     2461 gtgatgtcag gctgtttggc ttcagaacgg ttttcttgga aggcaaagca gaatgcggta
     2521 aatgccgcag catagataag atatttgccg gttttcttca ttttctatcc tttttctgtc
     2581 aactcaggat taaacctatg gaaaatctta aaaattatgt attgagtaag aaaaatcata
     2641 atttaaattt agtttatcat agttgtttcg tttttgggat agctaaggta aaatatattt
     2701 catgtttact ttagatgatt gaatgaaggg gagcggaagg atatttatgg aaacctttaa
     2761 agacagactg gtttttttat ggaaaagcga agcgaagcag gcaaaaatcg catccgatat
     2821 tgaaatgacg attgcgggct tcagcaggat atggaatgaa ggcggtctgc cgaagtctga
     2881 aacattaaaa aaaatcaagc agttgaaggg gtgtagtatc gattggctgc tgaccgggga
     2941 gggtaatccg tttccggatg aagccccaaa aaaatccctt gcttacgata ccttgggaaa
     3001 tgaagtcgat acggacgagt ttgtctttgt gccgagatat gatattcggg cggctgcggg
     3061 atacgggcag tttgtcgatc atgaggaacc ggtatttaca atggcgttca gacggcattg
     3121 gattgagaat tatgttaccc gcgatacgaa aaacctgtct gtaatttccg tcaaggggga
     3181 ttcgatggag ggggttttga atgacggcga ttcgattttg gtcaatcatg gtgaaaatac
     3241 gccgagggac ggtctgtatg tgttgcggat taatgaaaat ctgctggtta aacgtttaca
     3301 gattgtaccg ggcgggatta tcaatgtgat ttctgcaaac gaggcttatc ctgcttttga
     3361 aatcaatttg aacgatttga ccgatgatgt ggagattatc gggcgtgtcg agtggttcgg
     3421 caggacgatt tgagtttggg gcttgaaatt gcaggcggtc aaacttatct attggaacaa
     3481 ttcctttttc aaaggcgaag cctgcttgcc tttaaagggg gtttgagaga gaatgcagaa
     3541 aatattatat taaggaataa caccatgtcg gatgaaagcc ctattatttt tactgacagc
     3601 tgctgtgcca aagttgccga tttgattgcc gaagaaaaca atcccgattt gaaattgcgg
     3661 gtttttgtca atggcggcgg ctgttcgggt ttccagtacg gatttacttt tgacgaaatc
     3721 aaaaacgacg acgattttga aattgagaaa aacggtttgg tctttttggt cgatccgatg
     3781 agctatcaat atctggtcgg tgcggaaatc gactatacgg aaagtttgca gggttcgcaa
     3841 ttcgtcatcc gcaatccgaa tgcggaaaca acctgcggtt gcggatcgtc gttttccgta
     3901 tgaccgcttg gtttgtgtga tgccgtctga acgttcagac ggcattttta cttttggaaa
     3961 atatattatc gggatgaatt cacatataat ccgattgttt gaagatgaat cgggtttccc
     4021 gaaaggaacg ggcggaacgg tatcaggcgt atttgttccc ttatgattga gatgagtaaa
     4081 gattaccgaa atgatttgta cgatgtatat gtttcctacc cgccccaagt ggatcgcggg
     4141 cttatccggg agtgcctcag ggagaatctc ggcgaggaaa aggcggaagg attgatcgaa
     4201 tcgctcgatt ccaaacctca agtgctggtt gaggaaaaat gcacttgggc gaaacgggaa
     4261 gagttgcatg attatttcag ctatttgggt ttggatatta ttacccgga
//
LOCUS       DAXHPG010000163         4260 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXHPG010000163 DAXHPG010000000
VERSION     DAXHPG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.163"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            536..2263
                     /gene="ilvB"
                     /locus_tag="ACFM6F_001861"
     CDS             536..2263
                     /gene="ilvB"
                     /locus_tag="ACFM6F_001861"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC6165224.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDVLGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            2274..2765
                     /gene="ilvN"
                     /locus_tag="ACFM6F_001862"
     CDS             2274..2765
                     /gene="ilvN"
                     /locus_tag="ACFM6F_001862"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC6165225.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2832..3125
                     /locus_tag="ACFM6F_001863"
     CDS             2832..3125
                     /locus_tag="ACFM6F_001863"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC6165226.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3205..4218
                     /gene="ilvC"
                     /locus_tag="ACFM6F_001864"
     CDS             3205..4218
                     /gene="ilvC"
                     /locus_tag="ACFM6F_001864"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC6165227.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRTDLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1107 a         1022 c         1075 g         1056 t
ORIGIN      
        1 caatatatta agtcgttgtt agccctcttg gttctgtcaa tgccgtccaa tacagtccgg
       61 tgcaatgagc cattttcagg aagtatggtt gggaatattt ttgatatatc aaaaataaaa
      121 tcaagttcaa tcaatatccc gagtctttat tgggatgaaa ccctgtaaat tttacaattc
      181 ttgatatcga agaagggttt ttcacatctg cccctcctac acaaactgtc aacaatttcc
      241 atctttcaaa aaaggtactc ctgattttat tcttttaaaa tcaatcaata atttctctgc
      301 tttcaaaaaa tattaaaaga tgaaagattt ttcttgattg aactgtcggt ttgcgttatc
      361 gtttctactc tttttcacaa actctgtgtt cctttccaat tgattggata atccgtctgt
      421 tgccgtgttc ccccaatgcg cgtaccctaa atcgctgctt ggtggaattg cattacaggt
      481 gctgtggcaa agcggtttgt ccctattggt ttgaaaccgt ataaaagagg tcgttatgca
      541 gttatcaggc gcgcaaatca tagtgcagag tctcaaagcc gaaggtgtcg agtacgtttt
      601 cggttatccc ggcggtgcgg ttatcgaaat ctacgatgcc cttttccaac tcaataaatt
      661 caagcacatt ctgacccgtc acgagcaggc ggcagtacac gcggcagatg cgtatgcgcg
      721 cgtcagcggt aaggtgggcg tggcattggt tacatccggc ccgggcgtta ccaatgcact
      781 gaccggtatt gctactgcct atacggattc gattccgatg gtggtcatca gcgggcaggt
      841 aggcaattcc ctgattggta cggatgcgtt ccaagaagtt gatacggtgg gtattacccg
      901 tccgtgcgtc aaacacaatt tcctggttac ggacatcaat gagttggcgg aaaccattaa
      961 aaaggcgttc caaattgccg caagcggccg accggggccc gtggtggttg atgtcccgaa
     1021 agatgttacg caggcgatgg cgaaattcag ctatcctcag gaagacattt ttatccgttc
     1081 gtatcaaccg gttgttcaag ggcatatcgg tcagattaaa aaggccgtgc agatgttggc
     1141 atctgccaaa cgtccggtcg tctattttgg cggcggcgtg gtgttgggta atgcttctga
     1201 agagctgacc cgatttgtcc gaatgacggg tgctccgtgc acgggtacgc tgatgggctt
     1261 gggcgcttat ccttccggcg accgccaatt cctcggtatg ctcggtatgc acggtactta
     1321 cgaggcaaac cttgccatgc agaatgcgga tgtcgttctt gccgtaggtg cgcgttttga
     1381 cgaccgtgtg gtttccgtac cgtccaaatt tttcgagaag gcgaagaagg ttatccatat
     1441 cgatgtcgat ccttccagca tcgccaaacg cgtgaaggcg gacattccga ttgtcggcga
     1501 cgtgaaaaac gttttgtccg agatggttgc gctgtggcaa aaacaagagt ccgtgccgtc
     1561 tgaagatgtt ttgggcaaat ggtggaaaac catagaggaa tggcgttccc gcgattgctt
     1621 gtggtttgac aacggcagcg aaattatcaa gccacaatat gtgattcaga agcttgccga
     1681 gattaccggc aattcggcaa tcatcacatc ggatgtaggg cagcatcaaa tgtttgcggc
     1741 tcaatattat cccttcgaac gtccgcgcca atggctcaac tccggcggtt tgggtacgat
     1801 gggcgttggt ctgccttatg cgattggtgc aaaacttgcc gccccggatc aagacgtatt
     1861 ctgtattacc ggcgacggct cgatccagat gaacatccaa gagctgtcca cctgtttcca
     1921 atatcggatt ccggttaacg tcattacgct gaacaacggt tatctcggta tggtacgcca
     1981 gtggcaggaa atatattacg gcggccgaga gtcggaaact tatttcgatt ctttgcccga
     2041 tttcgtcaaa cttgccgagg catacggtca tatcggcatc cgcgtggaca agaagtctga
     2101 tgtggaaggt gcgttgttgg aagcattgaa ccaaaaagac aggctggtgt ttatcgactt
     2161 cctgaccgac cagaaacaga atgtgatgcc catggtcggt aacggcaaag gtttggacga
     2221 aatggtactt ccgccgcata tgcgtgcgga cgaaaaggcg taaggagagg caaatgcgac
     2281 atatcttatc tgttctgatt gaaaacgaat caggtgcgat gagccgcgtg gtcggtttgt
     2341 tctctgcacg cgattacaat atcgattctt tggcggttgc gccgaccgaa gacaaaaccc
     2401 tgtcccgtat gaccatcgtt acccacggcg acgagcaggt catcgaacaa atcaccaagc
     2461 aactcaataa attgattgaa gtgattaaag tggtcgattt gaacgaaagc cgttttgtcg
     2521 aacgcgaact gatgttggta aaagtccgtg ccgccggcaa agaccgcgac gaatttttac
     2581 gcttgaccga aatctaccgg ggcagcatca tcgacgtaac cgaccgcagc tatacgattg
     2641 aaattaccgg ctcgacagac aagctggatt cctttttgga aacggtcgga cgcgcccaaa
     2701 ttttggaaac cgtacgcaca ggcgcggccg gcatcggtcg cggtgagcgt attttgaaaa
     2761 tttaacgccg taacctttca gacggcacgg tatttgaatg tcgtctgaaa aacgaacggc
     2821 aggagagatt tatgtcgaac attaaaattg tcgcactggt taccgtcaaa ccggaataca
     2881 cagaaacact ggcagcacag tttaaagaac tggtcaaagc cagccgtgca gaagagggca
     2941 acatcagcta cgatctccat caggaaatcg gcaaaccgaa ccgttttgtt ttcgtggaaa
     3001 attggaaatc tcaagcagct attgacgaac ataacgccag cgcgcatttc caagccttcg
     3061 tccagtccgt cgacggcaaa accgaagcgt tggaaatcgt attgatgaat gaagttgccg
     3121 attaagcggc acactctgtt taacccgtcc gaagccgctc aacattttaa ggcttcgaca
     3181 accatttacc ttaaaggaaa tcaaatgcaa gtctattacg ataaagatgc cgatctgtcc
     3241 ctaatcaaag gcaaaaccgt tgccatcatc ggttacggtt cgcaaggtca tgcccatgcc
     3301 gccaacctga aagattcggg tgtaaacgtg gtgattggtc tgcgccaagg ttcttcttgg
     3361 aaaaaagccg aagcagccgg tcatgtcgtc aaaaccgttg ctgaagcgac caaagaagcc
     3421 gatgtcgtta tgctgctgct gcctgacgaa accatgcctg ccgtctatca cgccgaagtt
     3481 acagccaatt tgaaagaagg cgcaacgctg gcatttgcac acggcttcaa cgtgcactac
     3541 aaccaaatcg ttccgcgtac cgacttggac gtgattatgg ttgcccccaa aggtccgggt
     3601 cataccgtac gcagtgaata caaacgcggc ggcggcgtgc cttctctgat tgccgtttac
     3661 caagacaatt ccggcaaggc caaagacatc gccctgtctt atgcggctgc caacggcggc
     3721 accaaaggcg gtgtgattga aaccactttc cgcgaagaaa ccgaaaccga tctgttcggc
     3781 gaacaagccg tattgtgcgg tggcgtggtc gagttgatca aagcaggttt tgaaaccctg
     3841 accgaagccg gttacgcgcc tgaaatggct tacttcgaat gtctgcacga aatgaaactg
     3901 atcgttgacc tgattttcga aggcggtatt gccaatatga actactccat ttccaacaat
     3961 gcggagtacg gcgaatacgt taccggccct gaagtggtca atgcttccag caaagaagcc
     4021 atgcgcaatg ccctgaaacg catccaaacc ggcgaatacg caaaaatgtt tatccaagag
     4081 ggtaatgtca actatgcgtc tatgactgcc cgccgccgtc tgaatgccga ccaccaagtt
     4141 gaaaaagtcg gcgcacaact gcgtgccatg atgccttgga ttactgccaa caaattggtt
     4201 gaccaagaca aaaactgatt gttttcaaac gggactgcct acacatcgtg taggcagttt
//
LOCUS       DAXHPG010000164         4167 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXHPG010000164 DAXHPG010000000
VERSION     DAXHPG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.164"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..188
                     /locus_tag="ACFM6F_001865"
     CDS             <1..188
                     /locus_tag="ACFM6F_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165228.1"
                     /translation="IFVNPLYRHSRKNKKIKNTKLKYRHSRAGGNLGLSVRKLIGKNG
                     FSNPETPDSCFRGNPVF"
     gene            complement(506..751)
                     /locus_tag="ACFM6F_001866"
     CDS             complement(506..751)
                     /locus_tag="ACFM6F_001866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165229.1"
                     /translation="MPSELQTAFPASQLQRERTHTAIPAKNRKSKKKPKIRHSRAGGN
                     PIRPVSVFFEFQVTSKPPFPQKRESRNSKLQEFIKND"
     gene            complement(764..1348)
                     /gene="rnhB"
                     /locus_tag="ACFM6F_001867"
     CDS             complement(764..1348)
                     /gene="rnhB"
                     /locus_tag="ACFM6F_001867"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC6165230.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPEIFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQTLF"
     gene            complement(1416..3311)
                     /gene="mnmG"
                     /locus_tag="ACFM6F_001868"
     CDS             complement(1416..3311)
                     /gene="mnmG"
                     /locus_tag="ACFM6F_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC6165231.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPEDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3636..>4167)
                     /locus_tag="ACFM6F_001869"
     CDS             complement(3636..>4167)
                     /locus_tag="ACFM6F_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HFC6165232.1"
                     /translation="PVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYGDLSGAALTQ
                     AAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITAVFRMLVLAW
                     VYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKLKMGKDPEFK
                     LSEHPGLKRRIKSDVW"
BASE COUNT          831 a         1016 c         1225 g         1095 t
ORIGIN      
        1 tgatttttgt taatccacta tatcgtcatt cccgcaaaaa caaaaaaatc aaaaacacaa
       61 aactgaaata tcgtcattcc cgtgcaggcg ggaatctagg tctgtcggta cggaaactta
      121 tcgggaaaaa cggtttttcc aaccctgaga ctccggattc ctgttttcgc gggaatccgg
      181 ttttttgagt ttcagtcatt tttgataaat tcttgcagct ttgagtttct agattcccgc
      241 ttttgcggga atgacgcgga aaagttgctg tgatttcgga taaattttcg tcacgcttaa
      301 tttctgtttt atccgataaa tgcctgcaat ctaaaatttc gtcattcccg caaaaacaaa
      361 aaatcaaaac agaagcctaa aatttcgtca ttcccgcgaa ggcgggaatc taggtctgtc
      421 ggtacggaaa cttatcgggt aaaacggttt tgccagccct gagactccgg attcctgttt
      481 tcgtaggaat ccggtttttt gagcttcagt catttttgat aaattcttgc agctttgagt
      541 ttctagattc ccgcttttgc gggaatggcg gtttggaagt tacctgaaat tcaaaaaaaa
      601 cggaaaccgg acggattgga ttcccgcctg cgcgggaatg acggatttta ggtttctttt
      661 ttgattttct gtttttcgcg ggaatggcgg tatgggttct ttctctttga agttgcgatg
      721 ctggaaatgc cgtctgaagt tcagacggca tttttgtgcc ggtttaaaac agcgtctgct
      781 gcgcgagcag gtttctgacg ggggcgaagt cgcggcgatg ttcgggcagc acgccgtatt
      841 gtttgagggc ttccagatgc tgcttcgtgc cgtaaccttt gtgtttgtcg aaaccgtatt
      901 ggggatggcg ttgcgccagt gcgtacattt ccgcatcgcg tgcggtcttt gccaaaacgg
      961 atgcggcgga gatttcgatg attttgctgt cgcctttgac gacggcttcg gcagggatgt
     1021 tcaaatgttc gggaatgcgg ttgccgtcga tgaatattct ttcgggacgc acagccaaac
     1081 cgtcaacggc gcgtttcatc gcgagcatgg tggcgtgcag gatgttgagg ctggcgattt
     1141 cttcgggcga ggcggcggca acgtgccacg caaccgcctg attttttatc atttcggcaa
     1201 gcgcgtcgcg ttttttctcg ctgagttttt tggagtcggt cagtccgggc aggtcgaata
     1261 tttccggaag gatgacggcg gcggcaaaca cgctgccgac taaaggcccg cgccctgcct
     1321 cgtccacgcc ggcggtcagt atgtgcatga tgtttcctgt cgggatggcg cgatgccgtc
     1381 tgaacggtgt ttcagacggc atcgcgccga tgtgtttatt tcgcgtcttt aaacccgcgc
     1441 ttcaaatgca ccatcagcaa tgccactgcc gcaggggtta cgccggaaat gcggcttgcc
     1501 tgcccgacgg tttcgggttt gtgctggttg agcttttgct gcacttctgc cgacaaacct
     1561 ttaactttgc tgtaatcgat atcctcgggc agctttaagg tttcgatgtc gcggcggctg
     1621 tcgatttctt cgttttggcg gtcgatatag ccttggtatt tgacttggat ttcgacttgt
     1681 tcgaccactt cggcggagag gcgttcagac ggcatcgcgc cttcgagcgt catcagcgcg
     1741 gcgtagtcga ggttcgggcg gcgcaggagg tcgtgcaggt tggcttcgcg gctgagcttt
     1801 tgcccgaata cgcggatttg ttcgtcttcg gcgagttttt gcggcgtgta ccacgttgtt
     1861 ttcaaacgtt ggatttcgcg ttcgatggct tcgcgttttt cgttgaacat gcgccattgc
     1921 gcttcaccca ccaagccgat tttgtagccg tcttcggtca ggcgcatatc ggcgttgtct
     1981 tccctgagtt gcaggcggta ttcggcacgg ctggtgaaca ttcggtaggg ttcgttcacg
     2041 cctttggtga tgaggtcgtc caccaatacg ccgagatagg cttgttcgcg gcgcagcagg
     2101 agcgggtctt gttcgcggac gtattgcacg gcgttcgcgc ctgccagcag tccttgcgcg
     2161 gcggcttctt cataacctgt cgtaccgttg atttgcccgg cgaaaaacaa tccggcaatg
     2221 gttttggttt cgaggcttgc tttgaggttg cgcggatcga agtagtcgta ttcgatggcg
     2281 tagccggggc gcaggatatg ggcgttttcc aaacctttca tactgcggac gagcgcgatt
     2341 tggatgtcga acggcaggct ggtggagata ccgttggggt agtattcgtg cgtagtcagg
     2401 ccttcgggtt ctaggaaaat ctggtggctg tctttgtcgg cgaagcggtt gattttgtct
     2461 tcgatagacg gacaataacg cgggcccacg ccttcgattt tgccggtaaa catcgggctg
     2521 cggtcgaagc ctgagcggat gatgtcgtgg gtttgcgtgt tggtatgcgt aatccagcag
     2581 gacacttggc gcgggtgcat ttcggcgttg ccgcgtacgg acatgacggg aacgggcgtg
     2641 tcgccgggct gttcggtcag ttgggagaag tcaatcgtgc gtccgtcgat gcgcggcggc
     2701 gtgccggttt tcagacggcc ttgcggcagg ttcaattcgc gcaaacgtcc gcccagcgat
     2761 ttggcggcgg ggtcgccggc gcgtccgccc tcgtagtttt ccaaaccgat gtggattttg
     2821 ccggacaaaa acgtgcctgc ggtcaacacc acggcgcgtg ctttaaactc cacgcccatc
     2881 gcggtgatta cgcctgaaat gcggtcgcca tcgagcgtta cgtcttcgac ggcttgttgg
     2941 aaaaggtcga ggttttcttg gttttccaac atttcgcgga tggcggcttt atataggata
     3001 cgatccgcct gcgcgcgcgt ggcccgtact gccgcgcctt tgctggcgtt cagacggcgg
     3061 aactggatgc cggatttgtc ggttgccaac gccatcgcgc cgccgagcgc gtcgagttcg
     3121 cgcaccaaat gccctttgcc gatgccgccg atggaagggt tgcacgacat ttgtccgagc
     3181 gtttcaatat tgtgtgtgag caaaagcgtc tgcgcgccca tacgggcggc ggcgagcgcg
     3241 gcttctgtgc cggcgtgtcc gccgccgacg acgataacgt cgtaggtttt ggggtaaatc
     3301 atgtgggtca tgtgtgtatt gcctgacggt gtttcagacg gcatttatag tggattaaca
     3361 aaaaccagta cagcgttgcc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa
     3421 gtaccaagtg aatcggttct gtactatctg tactgtctgc ggcttcgccg ccttgtcccg
     3481 atttttgtta aaccactata ttcaatatgc cgtctgaaaa acgaaatgga ttcaaaagta
     3541 aagggttggg attgtacgct tgttcgccct gtttttacag tgtgcggaaa gggaaaagcc
     3601 gcttcgcggg gaagcggctc cggtaagggc gggatttacc aaacgtcgga tttgatacgg
     3661 cgtttcaggc ccggatgttc ggaaagtttg aactcggggt ctttgcccat tttcagcttg
     3721 gcggtgtaat cgcgcagcag cataaacgcc agtggcgaga gcagcaggat ggcgacaagg
     3781 ttgatccacg ccataatgcc catcgccata tccgccatat cccagaccaa aggcacattg
     3841 gcaaccgcgc cgaaatagac ccacgccaaa accagcatac ggaaaacggc ggtaatcagc
     3901 caatggcttt tgatgaattg gacgttggac tcggcatagg catagttgcc gataacggtg
     3961 gaaaaggcaa acataaacag gatgacggcg aggaagcccg cgccccattg ccccacttgg
     4021 ctgacaatcg ccgcctgcgt cagcgccgca ccgctcaaat cgccgtaagg ctgttggtaa
     4081 atcaagatga tgaaggcggt gcaagaacaa acgatgatgg tatcgacaaa cacgcccagc
     4141 atttgaatca taccttgcga aacaggg
//
LOCUS       DAXHPG010000165         4156 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXHPG010000165 DAXHPG010000000
VERSION     DAXHPG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.165"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            337..1854
                     /locus_tag="ACFM6F_001870"
     CDS             337..1854
                     /locus_tag="ACFM6F_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6165233.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRLVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNQNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1915..3795)
                     /locus_tag="ACFM6F_001871"
     CDS             complement(1915..3795)
                     /locus_tag="ACFM6F_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC6165234.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDSALQKGWQ
                     KGLHSVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAENWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPISLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNLASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIETLETEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLKPASASDGIVSGIGGPDSNVQARTAGQGEATPYRFQVHPLYQGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETMRVENRQCALLDRHLCRLKAAAQALN
                     LPLPDGCENQIKQYIADLPDGAFRVKALLASDGISLSRAVLNHLADKQRVIISPAVLP
                     AQNYLRRFKTTHRTLFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
BASE COUNT          870 a          935 c         1350 g         1001 t
ORIGIN      
        1 tttgcgggaa tgacggcgac agggttgctg ttatagcgga ttaacaaaaa ccagtacggc
       61 gttgcctcgc ctcagctcaa agagaacgat tctctaaggt gctgaagcac cgagtgaatc
      121 ggtttcgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
      181 gctataaatg tgaaatccgc cctttgaaaa tcgggcggcg gttttttgcc tgcctgcttt
      241 cagacggcaa agccggtttt cacgggtttc tgcctgtttt tcggatggtt tgacgtgctt
      301 cggcggcgtg tttgccagaa aggtaaatga cagggtatgt tgtatttcag atacggtttt
      361 ttggttgttt ggtgtgcggc aggtgtttct gccgcctatg gggcggatgc gcccgcgatt
      421 ttggatgaca aggcattgtt gcaggtgcaa cggttggtgt cggataagtg ggcggaatcg
      481 gattggaaag ttgaaaatga tgccccgcgc gtggttgacg gggatttttt gttggcgcat
      541 ccgaaaatgt tggaacatag tttgcgcgac gcgctcaacg gcaatcaggc ggatttaatc
      601 gcttcgttgg cggatttgta tgccaagctg ccggattatg acgcggtttt gtacggcagg
      661 gcgcgggctt tgctggcgaa attggcggga aggccggcgg aggcggtggc gcggtatcgg
      721 gaactgcacg gggaaaatgc ggcagacgag cggattttgc tggatttggc ggcggcggag
      781 tttgacgatt tccgactgaa gtcggcagaa aggcattttg cggaggcggc aaaattggat
      841 ttgccggcac cggttttgga aaatgtgggg cgttttcgga aaaaaacgga ggggctgacg
      901 ggctggcgtt tttcgggcgg catcagtccg gcggtcaatc aaaacgccaa taatgccgcg
      961 ccgcagtatt gccggcaaaa cggaggccgg cagatatgca gtgtcagccg ggcggagcgg
     1021 gcggcagggt tgaattatga aatcgaggcg gaaaaactga cggcgttggc agataatcat
     1081 tatttgttgt tccgttccaa tatcggcggc acgagctatt atttcagtaa aaaatcagct
     1141 tatgacgacg ggttcggcag ggcgtatttg ggttggcagt ataaaaatgc acggcagacg
     1201 gcggggattt tgccgtttta tcaggtgcag ttgtcgggca gcgacggctt tgatgcgaaa
     1261 acaaaacggg taaacaaccg ccgcctgccg ccgtatatgc tggcgcacgg agtcggcgtg
     1321 cagctgtccc atacttaccg accaaacccg ggatggcaat tttcggtcgc gctggaacat
     1381 taccgccaac gctaccgcga acaggatagg gcggaataca ataacggcag gcaggacggg
     1441 ttttatgttt cgtcggcaaa acgtttgggc gaatcggcaa ctgtgttcgg cggctggcag
     1501 tttgtgcggt ttgtgccgaa acgcgaaacg gtgggcggcg cggtcaataa tgccgcctac
     1561 cggcgcaacg gtgtttatgc cggttgggcg caggagtggc ggcagttggg cggtttgaac
     1621 agtcgggttt ccgcgtctta tgcccgccgc aactataagg gcgttgcggc tttctcgaca
     1681 gaggcgcaac gcaaccgcga atggaatgtc tcgctggctt tgagccacga caagttgtcg
     1741 tataaaggta tcgtgcccgc gttgaattat cgtttcggca ggacggaaag taatgtgccg
     1801 tatgcgaaac gccgcaacag cgaggtgttt gtgtcggcgg attggcggtt ttgaatggtg
     1861 gaataatgcc gtccgaactt tgtggaaaca ggttcggacg gcatttttgc gcgttcaggc
     1921 aagggcggcg gcaaatacgc cgcgcaaggc gttggagagg cggatttctt cggcttcttg
     1981 cagtgttttt tgtgtgatgt gtgtttcgat tacttgattt gtttgcaaat atttttgcgg
     2041 ttcgtccaac acggcttggc gcattatgcc gtttaaaatg tctaaatcta aagagggcgt
     2101 gagccattgt ccgcgatgtt tgacgaacac gttgcttctg ccgccttcga gcaggatgcc
     2161 gtctgaattg aaaaacaggc tgtcgaacgc gccttgtgtt tcggcggttt gccacgcttg
     2221 gtcgaagaga gtgcggtggg tggttttgaa gcggcgcagg tagttttgtg cgggcaggac
     2281 ggcgggcgaa atgatgacgc gttgtttgtc agccaaatgg tttaaaacgg cgcgggacag
     2341 gctgatgccg tctgaagcga gcagggcttt gacgcggaac gcgccgtcgg gcaagtcggc
     2401 aatgtattgt ttgatttgat tttcgcagcc gtcgggcagg ggcaggttga gggcttgggc
     2461 ggcggctttc agacggcata ggtggcggtc gagtagggcg cattgcctgt tttccacgcg
     2521 catggtttca aaaatgccga agtcggggcg caattcgttg aggaaacggg ctttccagcc
     2581 gcattcgcga tattcggcgg cgggatcgct gtcgatgacg atgccggaac cgacgccgta
     2641 caccccttga tagagtggat gaacttgaaa ccggtacggc gttgcctcgc cttgccctgc
     2701 cgttcgggct tgcacgtttg aatctgggcc gccgatgcct gaaacaatgc cgtctgaagc
     2761 cgaagcaggt ttgagcgaca aggtgcggat aacgacgttg aacgtgcctt caaaccccaa
     2821 gccgccggaa cacgggttca aatagccaat gctgcccgta taaagtccgc gcgcttcggt
     2881 ttcgagcgtt tcgataatct gcatactcat ttttttgggc gcgccggtga tgctgccgca
     2941 ggggaaggcg gcgcggagga tgtcggcgaa cgaggtgtgc ggcaaggctt gggcttggat
     3001 ggtgctggtc atctgccaga cgctgccgaa acgcgatact ttaaacggct cgggtacgca
     3061 tactttgccg gtttgggcga ttttgccgag gtcgttgcgc agcaaatcga caatcatcac
     3121 gttttcggcg cggtttttcg ggtctgcttg caactcggcg gcgcggcgtt cgtcttgtcc
     3181 gtcgcccaaa atcggcgcgg tgcctttcat cggttcggtg ctgatggtgc cgtctgaagc
     3241 gaggttgagg aagagttcgg gcgagaaaca cagcgtccac gcggattgcc cctccgcatc
     3301 gggcaggtgg gacaagacgg cataggggac gggctggcgc aggcggcggt agaggctgat
     3361 gggattgccg taggcttgca ggtgcaggcg ggtggtgtag ttgatttgat aggtgtcgcc
     3421 gcgccggatg gcttcgtgga tttggcggat atggtcgagg taatcggctt cggatacgga
     3481 ggattgcggc gtggaaatgc cggcggggag gccgtctgaa tgtcgggcaa gccagttttc
     3541 ggcatcggtg tcggcgcagt cggcaaacca gtgcagggca agattgccgc cgcgttcgga
     3601 ctcgaccccc gtcagcggca aaccgaatcc gtagtctgca aacaacaccg aatgcagccc
     3661 tttttgccag cccttttgca gcgcgctgtc caaagcatcg agttcttcgg gacggaaaaa
     3721 acggctttcc acatgatttt gatagcgttt tgcgcggccg cttacggcat cgtcaaacag
     3781 ggcgaaataa ggcatggcaa ttcggggcaa atgttttgat tatacgctct ttttacacat
     3841 attttcagac gattcggcaa atatcggcaa aatgtaattt tatgtagaga aagtgggggg
     3901 cgaaggtgta aaattgtgaa aacaaattcc gatttccaac ctgaaataca ataaggagac
     3961 ctttatgaca gaccaccagc tgcaaccgtt tgaaaacgta gaattaggcg aaaagcaaga
     4021 ccagctccaa gtatagtgga ttaacaaaaa ccagtacagc gttgcctcgc tttagctcaa
     4081 agagaacgat tctctcaggt gctgaagcac caagtgaatc ggttccgtac tatctgtact
     4141 gtcttcggct tcgttg
//
LOCUS       DAXHPG010000166         4121 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXHPG010000166 DAXHPG010000000
VERSION     DAXHPG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.166"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..819
                     /locus_tag="ACFM6F_001872"
     CDS             <1..819
                     /locus_tag="ACFM6F_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6165235.1"
                     /translation="ATANKGKCQDGAIGAAVGEIVGEALVKNTDFSDMTPEQLDLEVK
                     KITAYAKLAAGTVAGVTGGDVNTAAQTAQNAVENNAVKAVVTAAKVVYKVARKGLKNG
                     KINVRDLKQTLKDEGYNLADNLTTLFDETLDWNDAKAVIDIVVGTELNRANKGEAAQK
                     VKEVLEKNRPYIPNKGAVPNMSTYMKNNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQ
                     GLLKTGDRFYLDGQHKNHLEVFDKNGNFKFVLNMDGSLNQMKTGAAKGRKLNLK"
     gene            828..1205
                     /locus_tag="ACFM6F_001873"
     CDS             828..1205
                     /locus_tag="ACFM6F_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165236.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            1483..1857
                     /locus_tag="ACFM6F_001874"
     CDS             1483..1857
                     /locus_tag="ACFM6F_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165237.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            1943..3697
                     /locus_tag="ACFM6F_001875"
     CDS             1943..3697
                     /locus_tag="ACFM6F_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6165238.1"
                     /translation="MPSEKLTIFQTAFVMQVNIQIPYILPRCVRAEDTPYACYLKQLQ
                     VTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTAGAGAGAALGLNGAAAAA
                     TDAAFASLASQASVSLINNKGNIGNTLKELGRSSTVKNLMVAVATAGVADKIGASALN
                     NVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIK
                     QLDQHYITHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIVGEALVKNTDFSRMSATE
                     IEKAKAKITAYSKLVAGTASAVVGGDVNTAANAATVAVENNTLYPRCVGAKCDEFQKE
                     QQKWIRENPEEYREVLLLQTGFIPIIGDIQSFVQAQTAADHLFALLGVVPGIGESIQA
                     YKVAKAAKNLQGMKKALDKAATVATAQGYVSKTKIKIGQTELRVTAATDKQLLKAIGE
                     GRDTTGKMTEQLFDSLAKQNGFRVLSGGKYGGNNGFDHVWQAADGSVVLIVESKQIRN
                     GTVQLNPNGAGGYTQMSREWIKQVVKSLPDGSPAKAVVLKANQNGKLKTAIAGVDRQT
                     GKAVILSVKVPSKTNIRR"
     gene            3700..>4121
                     /locus_tag="ACFM6F_001876"
     CDS             3700..>4121
                     /locus_tag="ACFM6F_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HFC6165239.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPESIDYVDEKKGVP
                     LAAMKYILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNR"
BASE COUNT         1334 a          754 c          981 g         1052 t
ORIGIN      
        1 gcgacggcga ataagggtaa gtgtcaggac ggtgcgattg gcgcagccgt gggtgagatt
       61 gttggggaag ctttggttaa aaataccgat tttagcgata tgaccccgga acaattagat
      121 ctggaagtta agaaaattac cgcctatgcc aaacttgcgg caggtacagt tgcaggcgta
      181 acgggaggag atgtcaatac tgctgcacaa accgcacaaa acgcggtaga aaataatgcg
      241 gttaaagctg ttgtaactgc tgcaaaagtg gtttataagg tagccagaaa aggattaaaa
      301 aacgggaaaa tcaacgttag agatttaaaa cagacgttga aagacgaagg ttataattta
      361 gccgacaacc tgaccacctt attcgacgaa acattggatt ggaacgatgc caaagccgtt
      421 attgatattg tcgtcggaac agagctgaat cgcgctaata aaggggaagc ggcacaaaag
      481 gtcaaggaag ttttagaaaa aaatcgtcct tatatcccta ataaaggtgc tgtaccgaat
      541 atgagtacat acatgaaaaa taatcctttt ggaaaacagc tggctcaaat ttcagaaaag
      601 acaacgcttc cgacgcagca agggcagtct gtcttcttgg taaaaagaaa ccaagggtta
      661 ttaaaaaccg gtgataggtt ttatttagat ggccaacata aaaatcattt agaggttttt
      721 gataaaaatg ggaactttaa gtttgttcta aatatggatg gttcgcttaa ccaaatgaaa
      781 actggggcag caaaaggtcg taaattaaac ttaaaatagg aaattttatg gaaacattga
      841 atgatataaa aaaaatcttg attaatgtgg ggctttatca agggtttgat ttgacagatc
      901 caaaagtatc agaagaagtt aatcatgaaa cagctaatat gaaatggatt aaagattata
      961 cttcagacgg gaattgggat aatgaattta aggaggattt aaaaaacttt ttagattata
     1021 tggaagtatg ccaattagcc ctaaacgata aaaatttcaa aattgccagt aattctttat
     1081 ttatggctat gatttacgca ggtaatctat ctcttatatt tgattcaata aaaactgata
     1141 tatcaacatt attgagtgct gagtataaaa agaatagttt ttcatggcca tctcttgatg
     1201 aatagaaagc aagttgtagc ctgcatgaaa tctaaaaccc atgcataagg tgtgggcttc
     1261 agtatacgcg ttccatgatt tacggccata tgccgtctga aaagctcaat tttttcagac
     1321 ggcatttgtt atgaaagtaa atatttagat tccctgtata ctgtttagac tcgtgtgtgc
     1381 tgagtaagct gtagtctgca tgaaacctaa aactcgctca aaattaagct aagacattag
     1441 cagggcaagg gcgaaaattg aatcttaaat aaggtgattc agatgaaaaa ttttaatgta
     1501 gtaaaagaaa gtttaagaga gttaggaatt aaacaaggat ttgatcttta tgagaaagcc
     1561 acaactgaaa aattgaatag tgaagatcct cttgacttac aatggctttc taactattca
     1621 tctgattgga atgatgaatt agaagaagac tttgattctt tttttcagca tatgaaggaa
     1681 tatcaatatg ctattgacaa tgaagacatt aaatctgcat gtagttcact atgtgaagct
     1741 atgctctatg ttggtaatat taaaaatttt tttgagtttc tcaaaagcga tatgattaga
     1801 ctgttgagag gtgaaagtaa aacaacagac tttcaatggc cgcaatttga tgaatagcag
     1861 caagctgtag cctgcatgaa acctaaaatc catgcgtaag gtgtgtgctt cagcacgcac
     1921 gcgttccatg atttacggct caatgccgtc tgaaaagctc acaatttttc agacggcatt
     1981 tgttatgcaa gtaaatattc agattcccta tatactgccc agatgcgtgc gtgctgaaga
     2041 caccccctac gcttgctatt tgaaacagct ccaagtcacc aaagacgtca actggaacca
     2101 ggtacaactg gcgtacgaca aatgggacta taaacaggaa ggcttaaccg gagccggagc
     2161 agcgattatt gcgctggctg ttaccgtggt tactgcgggc gcgggagccg gagccgcact
     2221 gggcttaaac ggcgcggccg cagcggcaac cgatgccgca ttcgcctcgc tggccagcca
     2281 ggcttccgta tcgctcatca acaacaaagg caatatcggt aacaccctga aagagctggg
     2341 cagaagcagc acggtgaaaa atctgatggt tgccgtcgct accgcaggcg tagccgacaa
     2401 aatcggtgct tcggcactga acaatgtcag cgataagcag tggatcaaca acctgaccgt
     2461 caacctggcc aatgcgggca gtgccgcact gattaatacc gctgtcaacg gcggcagcct
     2521 gaaagacaat ctggaagcga atatccttgc ggctttggtg aatactgcgc atggagaagc
     2581 agccagtaaa atcaaacagt tggatcagca ctacattacc cacaagattg cccatgccat
     2641 agcgggctgt gcggcagcgg cggcgaataa gggcaagtgt caagatggcg cgataggcgc
     2701 tgcagtcggt gagattgttg gtgaggcttt ggttaagaat actgatttca gtcgtatgag
     2761 tgcgaccgaa atcgaaaaag ctaaagcgaa gattactgcc tattcaaaac tggttgccgg
     2821 cactgcgtct gccgttgtag gcggggatgt gaatacagcg gcgaatgcgg ctactgtggc
     2881 ggtggagaat aatactttgt atcctagatg cgttggtgca aagtgtgatg aatttcaaaa
     2941 ggaacaacaa aaatggatac gtgaaaatcc tgaagaatat cgagaagttt tgcttcttca
     3001 gacaggattt attccaatta tcggtgatat acagagtttt gtacaagcac agaccgctgc
     3061 cgatcacctg tttgctttgc tgggtgtggt tccgggtatc ggtgaatcga tacaggccta
     3121 taaagtagcg aaagcggcaa aaaatttaca aggcatgaaa aaagccttgg acaaggcagc
     3181 aaccgttgcc actgcacagg gctatgtcag taaaaccaaa atcaaaatcg gtcaaactga
     3241 attaagggtt actgcagcaa ctgacaaaca attgctgaaa gctattggcg aaggaaggga
     3301 cacgacaggt aaaatgaccg agcagttatt tgactcttta gctaaacaaa atggcttcag
     3361 agtgctttcg ggcggcaaat acggcggaaa taacggtttt gatcatgtat ggcaggctgc
     3421 cgatggtagt gttgttttga ttgtagaaag taagcagatt aggaacggta cggtacagct
     3481 gaatccgaat ggtgcgggtg gatatacgca gatgagtcgt gaatggatta aacaagttgt
     3541 aaaaagttta cctgatggta gtcctgctaa ggcagttgtc ttaaaagcaa atcagaacgg
     3601 caaattaaaa acggcaatag caggcgttga tcgtcaaaca ggtaaggccg ttattctttc
     3661 tgtcaaagtt ccttctaaaa ccaatataag gagataacaa tggggcacaa tatgatgacc
     3721 acccaaaaat ggtatgaaca tattactaat gtaatcatag gcaatactgc taatttcaat
     3781 agcggttgcc ccgaatctat agattatgta gatgaaaaaa aaggcgtgcc gcttgcagcg
     3841 atgaaataca ttttaatgta cactgaagct gcggcttccc atgcctatct atttgaacat
     3901 gatcttaaga aattcaagca atatgcttat gttgcaggaa agttgggtat tttgcagagt
     3961 gtagatgatg aagaccccga acccttcttc tttccctgcg acatgctcaa cattcaagat
     4021 ccgatgtttc tgatgctgat gagcgacagc ccgcagctgc gcgagttttt ggtgcgcaat
     4081 atcgacaaca tcgccaacga tacagaagcc ttcgtaaacc g
//
LOCUS       DAXHPG010000167         4062 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXHPG010000167 DAXHPG010000000
VERSION     DAXHPG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.167"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(126..1376)
                     /gene="glyA"
                     /locus_tag="ACFM6F_001877"
     CDS             complement(126..1376)
                     /gene="glyA"
                     /locus_tag="ACFM6F_001877"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC6165240.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     ETQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1531..1794)
                     /locus_tag="ACFM6F_001878"
     CDS             complement(1531..1794)
                     /locus_tag="ACFM6F_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165241.1"
                     /translation="MSNQTKIQAIKQASEQIFAICETPNTALQAIHLILRHGGAGELS
                     WQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLPLGSLVLT"
     gene            complement(1857..3632)
                     /gene="ggt"
                     /locus_tag="ACFM6F_001879"
     CDS             complement(1857..3632)
                     /gene="ggt"
                     /locus_tag="ACFM6F_001879"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFC6165242.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLIEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPSKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3800..4062)
                     /locus_tag="ACFM6F_001880"
                     /pseudo
     CDS             complement(3800..4062)
                     /locus_tag="ACFM6F_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
BASE COUNT         1016 a          961 c          957 g         1128 t
ORIGIN      
        1 aacatcccct aaatgtcttg gtgggaattt aggggatttt ggggaatttt gcaaaggttt
       61 ccgcctgaaa cattatgaga tttcaggcgg cattggattg cttagcggaa tatttttaaa
      121 aaggcttacg cgccgtaaac ggggtattta ttgcacaaag cagttacttg ttcgcggact
      181 ttggcgaggt tggcttcgtc ttcggggtta gacaatacgt cggcaaccaa gttcgccaat
      241 acgcgcgcgt cggcttcgtt aaaaccgcgt gtggtcatgg cggcggaacc gatgcggatg
      301 ccggaggtaa cgaagggttt ttccggatca ttcgggatgg cgtttttgtt gacagtgatg
      361 tgcgctttgc ccaaagcagc ttcggcggct ttgccggtaa ttttcatcgg ttgcaggtca
      421 acgaggaaaa cgtggctttc ggtgcggccg gaaacgatgc gcaaaccgcg tttaaccaac
      481 tcttccgcca tggcggctgc attgattttc acttgttttg cgtattgttt gaactcgggt
      541 tgcaatgctt ctttaaacgc cacggctttg gcggcgataa cgtgcatcag cgggccgcct
      601 tgcaggcttg ggaagatgga agagttcaac gctttttcgt gggtattgtc gcggcacaaa
      661 attacgccgc cgcgaggacc gcgcagggtt ttgtgggtgg tggtggtaac gaagtcgcag
      721 aatggcaccg ggttgggata ttcgccaccg gcaatcagtc cggcgtagtg cgccatgtcg
      781 acaaagaggt atgcgccgac tttatcggcg atttcgcgga attttgccca gtcgatttgc
      841 aacgcgtagg cagacgcacc cgccacaatc attttgggtt tgtgttcgag cgcgaggcgt
      901 tcgacttcgg cataatcgag cacttcgttt tcatccaaac cataagtaac ggcattgtag
      961 agtttgcctg agatattaac gctcgcgccg tgggtcaggt ggccgccgtg cgccagagac
     1021 atacccaaaa tggtgtcgcc tggttttaaa acggaagcgt acacggcttg gttggcttgc
     1081 gagccggagt gcggttggac gttggcgtac tgcgcgccga acagtttttt gacgcggtca
     1141 atcgccaatt gttcgacaat atcgacgtat tcgcagccgc cgtagtagcg tttgccgggg
     1201 tagccttcgg cgtatttgtt ggtcagctgg gaaccttgcg tttccattac ggcgcagctg
     1261 acgtagtttt cagaggcaat cagctcgacg tggtcttgct ggcgttggtc ttcttgggca
     1321 atggctgctg ccaaatcggg gtcatattgt gcgagggtaa cgctttttga aaacatgttc
     1381 tcggctcctt tgtgtaatca gggtatcatg agtgtttttt gtataaaaaa atacttcaaa
     1441 acctaaggca gatagtccat aatgcgtaaa ttttctttgg cattatcagg taatttattt
     1501 aacatgctgg tttttagcgt ctcattacct ttatgttagt acaaggctac ccaaaggcaa
     1561 tacatcaaat ggtaaatttt cggcagtttg tgcaaaatca atcaaataac cagcaccaac
     1621 cacatcttta tcagccataa cccgctgata caccacttgc caagacaact ctcctgcccc
     1681 accatgtctt aaaatcagat gaatggcttg taatgctgtg tttggcgtct cacaaattgc
     1741 gaagatctgc tcagaagctt gcttgatggc ttgtattttt gtttgattac tcacaatttc
     1801 cccctatttt aatattaact taatgcggtc aaattcacaa aatacaagct tcacctctaa
     1861 tcacccatca ctcgaccttc tcggcgtgga tcggcaccac caaccagcct gcttggctcg
     1921 ataataatgg cttgaacacc tgaatttagc tcacgcacgt cagtcttata gcccaaatca
     1981 tttaatgctt gttgccactg gagggcggtt gtacccgttt ctagttcata gctaccaaag
     2041 cgatttaata aattgggtgc actgatggca tcttggatat ccatattcca gtcgctatgt
     2101 gccacaatcg tcttagcgac atagccaatg atacggctac cacctgggga gccgattgcc
     2161 atataaggct tgcctgcttt aaatacgatg gttggtgcca ttgaggagcg tggtctcttg
     2221 ctgggctcga cacgattggc gacctgtttg ccctgcttta ttggctcaaa actaaagtct
     2281 gtcagctcat tattcagcag gtagccattt gccatcaaag ttgagccaaa cgcattttca
     2341 atggaagtcg tcattgatag cacattgccc gccttatcca caattgatat atgactggta
     2401 gaaggtaact caatcgcttg tgaggacacc cactcatgaa taaaatcgcc tgcagatacg
     2461 ctaggcaatg ccttatccga ctgctcaagc agctggctgc gatgttttag gtagtcttta
     2521 gaaatcaact ggcgaatggg tactggtaca aaatcagggt cgcccaaata tacatcacga
     2581 tccgcaaacg caagcctaga agcgtcgccc aagagacgta aaccttcagc atcatacccc
     2641 acctgattgg gtgaaaattc atttaaaatc cccaaaattt gacccacagc aatcccacct
     2701 gagcttggtg cacccatacc gcatacttca taaatacgat aagtcacaca aacaggcggg
     2761 cgttccacca cttgataatc agataaatct tgtaaggata attgaccggg gttatcctta
     2821 gcattttgga caactgaaac gatattttgg gcatatttac cagtatgcag agcttttgca
     2881 ccttgagctg ctaacgcctg aacactgtca gcaaattcta aatttttcag caagctgcct
     2941 gcttgtagcg gcacaccatt cggcaaaaaa taagcggctg tttttggata gcgtgccaaa
     3001 tgctgctgat tttgctcaac cgagatggca agccttggcg acacctcaaa gccttgtttt
     3061 gccaagcgga tcggtgtatc aaataatttt ccccaaggca atacaccgta tcgctgatgt
     3121 attgtctcca tcagtttagg gatagcaggc gtacccaccg agcgaccacc gaccaccgct
     3181 tccataaatt tcaatggttg accatcttta tccaaaaata attccggcgt cgcacgcatc
     3241 ggtgccgtct cacgcccatc aaatgtggtc aatgttttgg cggtattatc ccaatacaac
     3301 acaaatgcac caccgcccaa gcctgacgac tgtggctcta ccaagcttag tgtcgtctgc
     3361 accgccacca tcgcatctgc agcgctaccg ccttgcttta agatatcata gccagcttgt
     3421 gttgctaatg gattggctga cgctaccata aaatcacttg caatcacctg cttttgttcg
     3481 atcagtcccg ttgcatgttc aggcgtgtga gcgtctgcac ctgtgatgac agcagaatga
     3541 gtattaacct taccttgatt ggcatggatg acttgacatc cagagattgt catagacatt
     3601 atcaatgcag tcaataaata tgttttagcc acaagcactc cttcgcctga gtttgattga
     3661 tgattcatac aaggcatgct gattattgta tttaatatgg ctaaataatt caatccaaac
     3721 tatcaatctt gaccatcaaa aaaagaccgc taatgtcatc agcagtcttt tttgatattt
     3781 attttaagat attaagtaat cagacctttg ggctatgctc ttcaatgagt ggttttagct
     3841 cacctgattg gtacatttgt aggataatat cactaccacc gattaactca ccattaaccc
     3901 aaagctgtgg aaaggttggc cgattggcga tgagtggtag agtactgcga atttctgggt
     3961 tttctaggat attaacaaaa gcaaagggtc tgccaattgg gtcagcacct ctactgcacg
     4021 cgctgaaaat ccacattggg gaaactgggg cgtgcctttc at
//
LOCUS       DAXHPG010000168         4058 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXHPG010000168 DAXHPG010000000
VERSION     DAXHPG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.168"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(1169..1606)
                     /gene="rnhA"
                     /locus_tag="ACFM6F_001881"
     CDS             complement(1169..1606)
                     /gene="rnhA"
                     /locus_tag="ACFM6F_001881"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC6165243.1"
                     /translation="MDKTVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1720..2589
                     /gene="tehB"
                     /locus_tag="ACFM6F_001882"
     CDS             1720..2589
                     /gene="tehB"
                     /locus_tag="ACFM6F_001882"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC6165244.1"
                     /translation="MKEWIVGQSGELFCFGQMPVWKAENLPEVLLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSMYCVAEDYFH
                     KKYGMSATHSAVAAAQDIVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKRPE"
     gene            complement(2639..3445)
                     /gene="thiD"
                     /locus_tag="ACFM6F_001883"
     CDS             complement(2639..3445)
                     /gene="thiD"
                     /locus_tag="ACFM6F_001883"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC6165245.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCEAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          871 a         1010 c         1141 g         1036 t
ORIGIN      
        1 ggtttttgtt aatccactat agtttaattt gtctgtccgc attgccgcta attgtaccac
       61 ttcatcgtat ttccttttcg gttgaaaccc cgccctttag ggcggcagga tcagacttta
      121 tttgggaggg ggtgtaaccc cttccaaatc aggacgacac atagggaggt gctttatgtg
      181 tcgtcctgtg tgttgaaata tatcggacgc gtttgaacag ttgtgcggca aactgtttta
      241 gcggcaggca agagatgccg tctgatgccg tctgaagccc attgtccgtc attcccacac
      301 aggcagttgc tacggttgct gaagcccctt agccgtcgcg caggcggttg ctacggttac
      361 taaaatcccg tcattcccgc gcaggcggga atccagtccg ttcggtttca gttgtttctg
      421 ataaatcttg cagcgttggg tttttagatt cccgctttcg cgggaatgac ggggcggtcg
      481 gatgccgcct gaaactccgt cattcttgca acttttcgtc attcccgtga aagcgggaat
      541 ctagaccttt taaattttca cacggctttt gaatattgcc gttacccgaa ggtctagatt
      601 cccgcttgcg cgggaatgac ggtagagcgg tttcagttgc tcccgataaa tgccgcaatc
      661 tcaaatcccg tcattccctc aaaaacagaa aaacaaaatc agaaacctaa aatcccgtca
      721 ttcccgcagc tcttcgtcat tcccgtgaaa gcgggaatct agacctttta aattttcaca
      781 cggcttttga atattgccgt tacccgaagg tctggattcc cgcctgcacg ggaatgacgg
      841 cagagcggtt tcagttgttt ctaaataaat ttttgcagcg ttggattttt agattcccgc
      901 tttcgcggga atgacggggc ggtcggatgc cgcctgaagc ccgacaaagc ccccaatgcc
      961 gtctgaaacc tcgtcattcc tctgctgtcc gcaacttttc gtcattcccg caaaagcggg
     1021 aatctagagc ctctaaattt tcagacggct tttgaatatt gccgttgccc gacggtttgg
     1081 attcccgcct gcgcgggaat gacggtggag gacgatgccg tctaaagccc attagcggtt
     1141 tcagacggca tttttgccgg agcggcagtc aggaaaactg cgctgcgcca cggtttgcca
     1201 aatcgtcggc gcgttcgttt tccgcgtgtc ccgcgtgtcc tttaacccaa gtccagctga
     1261 cttggtgctg cccaaccaga acgtcgagtt ctttccacaa gtcgtcgttt ttgacgggct
     1321 gtttggcggc ggttttccag ccgttgcgct tccaaccgtg tatccagttt tccatgccgt
     1381 ttttgacgta ttgcgagtcg gtgcagatga tgacggtgca gcggcgtttg agcgatttca
     1441 gtccttcgat gacggcggtc agctccatgc ggttgttggt ggtttgcgct tcgccgccga
     1501 aaagttcttt ttcgcggctg ccgtagcgca tcaacacgcc ccagccgccc gcgccgggat
     1561 tgcctttgca cgcgccgtcg gtgtaaaggt aaacggtttt gtccattatt gtgcctttgt
     1621 gtggtgtccg gcgtgattat aagcggtttg tgcgccgtgc cgtctgaaag attgcggcgg
     1681 attaaaatag gcatataatg tgcaacttta tttttgaata tgaaggagtg gatagtgggt
     1741 caaagcggcg agttgttttg tttcggacag atgcccgtat ggaaggcgga aaacctgccg
     1801 gaagttttgt tgtcgggcta ttcgtccgaa ggaggggaat gggtctgcct gaatgtgttg
     1861 cagggcgatg tcgaagtccg cacgcaggac ggggcggcgg aggtttggtc ggcagaaagc
     1921 ggcgattgcg tgtttgcgcc gcagcaggtg ttttcggtca aaccgaaaac ggacgatgcc
     1981 gaaatccgtt tgtcgatgta ttgtgtggcc gaagattatt tccacaaaaa atacggcatg
     2041 agtgccacgc attctgcagt ggcggcggct caggatatcg tacctgcggg cagggcgctc
     2101 gatatgggct gcgggcaggg gcgcaacgcg ctgtttctcg gtttgaaggg gtttgaagtt
     2161 actgcagtcg atcacaatcc cgccgccttg gcaaacgtgg cggagctggc agaggcggag
     2221 ggtttgaacg tccgcacgct ggaatatgat ttgaacgccg ccgccttgca gggcgagttt
     2281 gattatattg tggcaacagt ggtgctgatg ttcctgatgc cgcagcgcgt acccgacgtg
     2341 attgccgata tgcaggcgca tacggcggcg ggcgggtaca acttgattgt atcggcaatg
     2401 gatacggcgg atttcccctg cccgatgccg ttccccttca aatttaaaga gggagaactg
     2461 aaggattatt atcgggattg ggagctggtc gaatataaag aagaattggg cgcgatgcac
     2521 gccaaagacg aaaacggcaa tccgatacgg tttaaatttg taaccatgct ggcgaaaagg
     2581 cctgaataat cttcaaaaat gccgtctgaa cattttgttt cagacggcat ttttacggtt
     2641 agtcccgata cgcccaatga ttgaccggcc cgtgtcccgc gccgatttcc aaagggtttg
     2701 agattgccgc cgtgatatag gccttggcag tctgtacggc ttcgcaaacg tccgagcctt
     2761 ttgccaattc agcggtaatg caggcggaaa acgtgcagcc tgtgccgtgc gtgtgggcgg
     2821 tcggaaagcg cgggctgtcc aattccagcg tttcgttttg cgtaaacagc caatccgtgc
     2881 agcgtccgct gctgctgccg ttcaaatgtc cgcctttgat aacgacattt ttgacaccgt
     2941 aatcaagcag gatttttgcc gcacgttccg catctttacg gttttcgata tgcacgccgg
     3001 tcagggcttc cgcctcgggc aggttggggg tcaatacatc cgtatcggga agcagcaggc
     3061 gcgtcagtgc cgcaacggcg gaatcctgca acagcggcgc accgcctttg gcaatcatca
     3121 cagggtcgag tacgcgcctg ccgaagtcac agtgtttcag cttgtcggca acgcattcga
     3181 tgatttccgc cgtgccgagc attcctattt tgtaggcgcg gatgtcaaag tcttccctga
     3241 ttgcttggat ttgtgcggtg atggtttcgg tcgggacgag atgaaccgcc gacacgccca
     3301 aggtattttg cgcggtaacg gcggtgatga cgcacgttcc gaacacgccg cgcatctgaa
     3361 acgttttcaa atccgcctga atgcccgcac cgccgcccga atccgaaccg gcgatggtaa
     3421 gcgtttgcac aaaagagctt ttcatttgtt cctccttaat attgtgtccg ataaaaaggg
     3481 ctaatgcctg taaaaaagca cggcgatgcc gtctgaaaac cgcagcgcgg ggtttgtggt
     3541 gcattatatg ccgtattgca ggacaaggca cagggcaggg cggcggggtg catcgtagca
     3601 actgtatttt tgcccgacgg ggtgaaaaat acagttgcta cacccactga taatgaaaaa
     3661 agagagcaat gccgtctgaa ccgcgtgttg cggcgagtat aaaccggcac gctttattcg
     3721 tcattcccac aaaaacagaa acctaaaatt ccgtcattcc tacgaaagcg ggaatccagt
     3781 gtgttcggtt tcagttgttt ttgggtttcg ggtgatttcc aaatcatcat tcccgcgcag
     3841 gcgggaatct agcccttggg gcagcggcaa tatttataaa gccgtctgaa aattcagaag
     3901 ttctagattc ccgttttcac gggaatgacg aagggttgtg ggaatgacga agtttcagac
     3961 ggcatcgccc cgccccgtca ttcccgcata agcgggaatc caaaccattg ggcagagcag
     4021 ccttaaaggt tataaaagac tcgtcattcc cacgaaag
//
LOCUS       DAXHPG010000169         4025 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXHPG010000169 DAXHPG010000000
VERSION     DAXHPG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.169"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..1707
                     /locus_tag="ACFM6F_001884"
     CDS             <1..1707
                     /locus_tag="ACFM6F_001884"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6165246.1"
                     /translation="LYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRYTADGKQ
                     AVGEIKRHSDGTPAENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYYFVKKKQQDSF
                     GYNTNFKLSTWLTFMKNRLSIAKELLTDDGIIVISIDDDGNAYLKILLDEIFGFENFI
                     GNLPTIMNLKGNNDEYAFAGTHEYTLVFAKNKDKSTFYEFPIDEDNFLEKWEEDEIGF
                     YKKGAPMRATGTEDKREDRPEMFYPFLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQ
                     KLKEKYENLGYNFILPIADKQWGRWRWGYSIKNKARLQTDIIVSQSKNGISLYKKQRP
                     ELDDLPTKKPKTIFYKPEYSSGNGTEQMKNLFGEKAFKNPKPEELIQDFITITTNESD
                     IVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQ
                     GGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQE
                     PEFQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFADCLNDDDKAVSRAFYQAEKKN
                     GE"
     gene            1697..>4025
                     /locus_tag="ACFM6F_001885"
     CDS             1697..>4025
                     /locus_tag="ACFM6F_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC6165247.1"
                     /translation="MANNKTLFEVIENRKAVYLEDGDDEKYRLPKFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPTHAKFLFTEKILQGDTVIPIRKVETFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPSQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYLAFLNWAENVQAVDFSFLTTFSTSLNDSDSDNANEQGKTRTEQALKFMQE
                     KGVEFAHLADWVKQNYQKHNVIITNSETNKTKTEKTDSETEKLLNNLEAADNPIRAIF
                     TVDRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAF
                     EGKQPNKRKFDNDMQHELRILEELFYYAHDEQSRYISELKNELRKDGYLPEKDDDKVL
                     ATFKLKSEFAANKDFRELLIWANKKIPNPNAKSNNADSLKANPQTLSFQVHGNQLLQE
                     TQFTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSK
                     LDIQNRNELQNNLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFI
                     GTKEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQND"
BASE COUNT         1457 a          688 c          787 g         1093 t
ORIGIN      
        1 ttatacacta aattaacccg aaaaagacaa gaaatctttt ttaatcaaac gcttgctttt
       61 gatgaaatcg atcggctttt tgacgcaaaa gcattctcaa aattctctcg ctataccgca
      121 gacggcaaac aagcggttgg cgaaatcaaa cgtcattcag acggcacacc cgccgaaaat
      181 ctcattatca aaggcaataa tctgatagcc ctacattcgc ttgccaaaca gtttaaaggc
      241 aaagtgaagt tgatttatat tgatccgcct tattattttg ttaaaaagaa acaacaagat
      301 agttttggtt ataacactaa ctttaaatta tctacttggc ttacatttat gaaaaatcgt
      361 ttatcaattg ccaaagaatt attaactgat gatgggatta ttgttatttc cattgatgat
      421 gacggaaatg cttatttaaa gattttgtta gatgaaattt ttggatttga aaattttatc
      481 ggaaatttac ccactattat gaatttaaaa ggtaataatg atgaatatgc ttttgcaggc
      541 actcacgaat acacactagt ctttgctaaa aataaagata aatctacttt ttatgaattt
      601 ccaatagatg aagataattt tttagagaaa tgggaagaag atgaaattgg gttttataaa
      661 aaaggagctc caatgcgagc aacaggaacc gaagataaaa gagaagatag acctgaaatg
      721 ttctatcctt ttttggtaaa aaataacaca gttagtacaa ttacagatga agaattttct
      781 caaatctata ataaagattt agaagtattc aatgatgatt ttattcaaaa actaaaagag
      841 aaatacgaaa atcttggata caattttatt ttacctattg ctgataaaca atgggggcgt
      901 tggcgttggg ggtattcaat aaagaataag gcaaggctgc agacggatat tattgttagc
      961 caatctaaaa atggaatttc cttgtataaa aaacaaagac ctgaattaga tgatttacct
     1021 acaaaaaaac ctaaaactat tttttataaa cctgaataca gtagtggtaa tggaactgaa
     1081 cagatgaaaa atctttttgg tgaaaaggca tttaaaaatc ctaaaccaga agaacttatc
     1141 caagatttta ttaccatcac aaccaacgaa tccgacatcg tcttagacta ccatcttggt
     1201 agtggcacaa ccgccgccgt tgcccacaaa atgaaccgac aatatatcgg tattgaacaa
     1261 atggattata ttgaaacgct tgccgttgaa cgcttgaaaa aagtgattga tggcgagcaa
     1321 ggcggtattt ccaaagccgt gaattggcaa ggtggtggcg agtttgttta tgccgaactt
     1381 gccccattta acgaaaccgc aaaacaacaa attttggctt gcgaagattc agacggcatc
     1441 aaaacgctgt ttgaaggttt atgcgaacgc tatttcttga aatacaacgt cagcgtaaat
     1501 gaatttagtc aaatcattca agagcctgaa tttcaatctt tgccattaga cgaacaaaaa
     1561 caaatggtgc ttgaaatgtt agatttaaat caaatgtatg tttcattatc cgaaatggac
     1621 gacgagcaat ttgcagattg cctgaacgat gacgataaag cggtaagccg tgcgttctat
     1681 caagcggaga aaaaaaatgg cgaataataa aactttgttt gaagtaattg aaaaccgcaa
     1741 agctgtgtat ttggaagacg gagatgatga aaaatacagg ctgcctaaat ttgtttcaaa
     1801 taatttgaaa tatccgtttt ttgaatggca aaaatcggct ttagaaaact ttgtgatttt
     1861 tgaccgcact tcaaaattaa aggattttcc cgatatcaaa aaccgcccta ctcatttgct
     1921 atttaatatg gcaacaggtg caggcaaaac gatgatgatg gcggcattga ttttgtatta
     1981 ttttgaaaaa gggtatcggc attttctgtt ttttgtgaat caaaacaata tcgtggataa
     2041 aacggaaaat aattttattg acccgacaca cgcaaaattt ttatttaccg agaagatttt
     2101 gcagggcgat acggtaattc ccattcgcaa agtggaaacg ttcagcccac attcagacgg
     2161 cattgaaatt aaatttacca gcattcaaaa gctgtataac gatattcaca ccgaacggga
     2221 aaaccaaacc acattggcgg atttgcacga attgaatctt gtgatgctgg gcgatgaagc
     2281 acaccattta aacgcgcaaa ccaaaggcaa aaaacaaggc gagttggatt tggaagtgga
     2341 attaaaagcc aatgcaggca atgccgaaat tgaacgcaaa ggctgggagc atatggtttt
     2401 ggaattgtta ctcaataaaa atggcaatcc cagccaaaat gtgctgttgg aatttaccgc
     2461 cacgctgcct gaaaatgctg acgtgcaaca aaaatacgcg gataaaatca tcacaaaatt
     2521 tggcttaaaa gaatttttgc aaaaaggtta taccaaagaa atcaatttgg tatccagtac
     2581 gctgggcaag aaagagcggg tgttgcacgc tttattattt gcttggtatc gccatcaaat
     2641 cgctttgaaa tatggcattg ccaatttcaa gcctgtgatg ttgtttagaa gtaagacgat
     2701 tgatgaatca aaagcggatt atctggcatt tttaaattgg gcagaaaatg tgcaggcggt
     2761 tgatttttcg tttttaacta cattttcaac aagcttgaac gatagcgata gcgataacgc
     2821 caacgaacaa ggcaaaaccc gcactgaaca agccctaaaa tttatgcagg aaaaaggcgt
     2881 tgagtttgca catttggcag attgggtaaa acaaaattat caaaaacaca atgtgattat
     2941 taccaactcc gaaaccaaca aaaccaaaac cgaaaaaacc gacagcgaaa cagaaaaatt
     3001 gctgaataat ttggaagcgg ctgataatcc gattcgtgcc atttttacgg tggacagatt
     3061 aaccgaaggt tgggacgttc tgaatttgtt tgatattgtg cgtttgtatg aagggcaaaa
     3121 cggcggcggt tcaaataaaa aatcaggcaa aacggctgcc gccaccgtat cggaaaagca
     3181 gttgattggt cgtggcgtgc gttattttcc atttgcgttt gaaggtaaac agccgaataa
     3241 acgcaaattt gacaacgata tgcaacacga attgcgtatc ttggaagaat tgttttatta
     3301 cgcgcacgat gagcaatccc gctatatttc agaactgaaa aatgagttgc gaaaagacgg
     3361 ttatttgcct gaaaaagacg atgataaggt attggcaaca tttaaactca aatctgaatt
     3421 tgccgctaat aaggatttta gagaattgtt aatttgggcg aataaaaaaa tccccaaccc
     3481 aaacgccaaa agcaataatg cagacagcct aaaagccaat ccacaaacgc tttcattcca
     3541 agttcacggt aatcaactgt tgcaggaaac gcaatttaca gccgatgaaa atgatgaaac
     3601 agcccgacaa atcggcacac aaaataattt tactcaaacc ataaaaatga gtgaaatgga
     3661 gcggcacatt ttcaataaag cattacatat caaagggaaa aatagtcaat ctttattcca
     3721 tttcaaccgt ttgcaaagca agctcgacat tcaaaatcgc aatgaattgc aaaataactt
     3781 gttgaaagac tggcaaattg aatttttagg attagggcaa gacaaacagg ttcgcctgga
     3841 cgataaactt gcaggctgcc tgaaaatctt ggaaatggtt gaaaaacatc tgaatgaaag
     3901 tgatatgccg tttatcggta caaaagaatt tacacctaaa aaattatggg aaattttcgg
     3961 cacgccaaaa caaaaatggg tcaaaaaaga tgatgtaaaa acgactattg ccacgcaaaa
     4021 tgatt
//
LOCUS       DAXHPG010000170         4008 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXHPG010000170 DAXHPG010000000
VERSION     DAXHPG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.170"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..632)
                     /locus_tag="ACFM6F_001886"
     CDS             complement(<1..632)
                     /locus_tag="ACFM6F_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6165248.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKHN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPSADFLELLSLCRDIHALTEGAFDPTVQVLWKLYADYFSAHPDAETPPPEN
                     SVKEALKRVGFGKVSFDDREIRFTEKGMGLSLNGIAQGYSGLTKTSTALPR"
     gene            complement(641..1435)
                     /locus_tag="ACFM6F_001887"
     CDS             complement(641..1435)
                     /locus_tag="ACFM6F_001887"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC6165249.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAAVSV"
     gene            1858..3192
                     /gene="gdhA"
                     /locus_tag="ACFM6F_001888"
     CDS             1858..3192
                     /gene="gdhA"
                     /locus_tag="ACFM6F_001888"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC6165250.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3334..3708
                     /locus_tag="ACFM6F_001889"
     CDS             3334..3708
                     /locus_tag="ACFM6F_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165251.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3752..>4008
                     /locus_tag="ACFM6F_001890"
     CDS             3752..>4008
                     /locus_tag="ACFM6F_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HFC6165252.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT         1090 a         1046 c          938 g          934 t
ORIGIN      
        1 cgaggcaacg ccgtactggt ttttgttaat ccactatagc cttgcgcgat gccgttcaag
       61 gacaaaccca tacccttttc ggtaaaacgg atttcacggt cgtcaaacga gactttgccg
      121 aagccgaccc gcttcagggc ttccttcacg ctattctccg gcggcggcgt ttccgcatcg
      181 ggatgggcgg aaaaataatc ggcatacagt ttccacaaaa cctgaaccgt cggatcgaac
      241 gcgccttccg tcagcgcgtg aatatcgcgg cacaggctca acagttccaa aaaatccgcc
      301 gacggcgaag tcagataacc gtcacggttc aggcggctga tcaggctgtc ttcacggtaa
      361 aggctgaaca ttttttccaa acgcgccact tccgccaaaa ccttattgac caaatccgcc
      421 gcctgtctgt cgtccacgcc gaacagacgc agctccgcac cggaacccag cgcgacacct
      481 ttccagaaaa acacattttc attgtgtttt tcatctttat tgcgtttttc atcatcggcg
      541 gcaaaaggat tcggcaaaaa agaaaccgcc gcgcccgccg ccgcaacggc ggcaactgtc
      601 aaaaaacgcc tgcgcccgaa atgcctgccc ataccgcctc ctaaaccgac actgccgcct
      661 tgatgtgcgg atgcggatcg tagccttcca actcaaaatc ttcaaatttg aaggcaaaca
      721 aatctttgac ttcaggattg attttcatca caggcaaggc gcgcggttcg cgttccaact
      781 gcaatgcggc ctgctcgaaa tggttgcggt acaggtgcgc gtcgccaaac gtatggacaa
      841 actcgcccgc ctccaatccg cacacttgcg ccatcatcat ggtcaacagc gcgtagctgg
      901 caatattaaa cggcacacca aggaaaatat ccgcgctgcg ctggtaaagc tggcaggaca
      961 gtttgccgtc ggcaacgtaa aactgaaaca gcgcgtggca gggcggcaat gccatttcat
     1021 cgaccaaagc cggattccac gccgatacaa tcaggcggcg cgagtcgggg tttttcttaa
     1081 tttgttccac cacattggcg atttggtcga tatgcctgcc gtcgggcgcg ggccagttac
     1141 gccactggta gccgtaaacc gggcctaagt cgccgttttc gtccgcccac tcgtcccaaa
     1201 tggaaacatt gttgtccttt aggtatttga tattggtatc gcctttgaga aaccaaagca
     1261 gctcgtggat aatcgagcgc aaatgcagct ttttggtcgt cagcagcgga aaacctttgc
     1321 ccaagtcaaa acgcatctga taaccgaata cggagcgcgt gcccgtaccg gtgcggtctg
     1381 atttgtccgt accgttgtcg aggacgtggc gcatcaagtc caaataggcc ttcatcatca
     1441 tctttcatca aattaaacgg cgcatattgt aacatttccg gccaacgccc gagtccggac
     1501 aaacgcacac gccgccccgc cggacgataa aaacgcagat acaggcaggc aggattgttg
     1561 gcaatttcag tccttttcca cagtaaaacc cagtgggaaa acaaaattac cttgattgga
     1621 atcaaaaaat ctagtttaat tacttagaat aaaatttcaa taatattttt atttacgaaa
     1681 ttaatttatg attattgatt aattatcggc aacaaacatc aaacatcgaa aacatggaaa
     1741 aaataatgtc aacaattttt gccaaatcgg gcttggcatc agaaaaaaat aggtttatat
     1801 tcccacctac aaatttgttt tcccattagt acactatcaa ccaaaaggag tatccgaatg
     1861 actgacctga acaccctgtt tgccaacctc aaacaacgca atcccaatca ggagccgttc
     1921 catcaggcgg ttgaagaagt cttcatgagt ctcgatccgt ttttggcaaa aaatccgaaa
     1981 tacacccagc aaagcctgct ggaacgcatc gtcgaacccg aacgcgtcgt catgttccgc
     2041 gtaacctggc aggacgataa agggcaagtc caagtcaacc ggggctaccg cgtgcaaatg
     2101 agttccgcca tcggtcctta caaaggcggc ttgcgcttcc atccgaccgt cgatttgggc
     2161 gtattgaaat tcctcgcttt tgaacaagtg ttcaaaaacg ccttgaccac cctgcctatg
     2221 ggcggcggca aaggcggttc cgacttcgac cccaaaggca aatccgatgc cgaagtaatg
     2281 cgcttctgcc aagcctttat gaccgaactc taccgccaca tcggcgcgga caccgatgtt
     2341 ccggccggcg acatcggtgt aggcgggcgc gaaatcggct acctgttcgg acaatacaaa
     2401 aaaatccgca acgagttttc ttccgtcctg accggcaaag gtttggaatg gggcggcagc
     2461 ctcatccgtc ccgaagcgac cggctacggc tgcgtctatt tcgcccaagc gatgctgcaa
     2521 acccgcaacg atagttttga aggcaaacgc gtcctgattt ccggttccgg caacgtggcg
     2581 caatacgccg ccgaaaaagc catccaactg ggcgcgaaag tgctgaccgt ttccgactcc
     2641 aacggcttcg tcctcttccc cgacagcggt atgaccgaag cgcaactcgc cgccttgatc
     2701 gaattgaaag aagtccgccg cgaacgcgtt gccacctacg ccaaagagca aggtctgcaa
     2761 tactttgaaa accaaaaacc gtggggcgtc gccgccgaaa tcgccctgcc ctgcgcgacc
     2821 cagaacgaat tggacgaaga agccgccaaa accctgttgg caaacggctg ctacgtcgtt
     2881 gccgaaggtg cgaatatgcc gtcgactttg ggcgcggtcg agcaatttat caaagccggc
     2941 atcctctacg ccccgggaaa agcctccaat gccggcggcg tagcaacttc aggtttggaa
     3001 atgagccaaa acgccatccg cctgtcttgg actcgtgaag aagtcgacca acgcctgttc
     3061 ggcatcatgc aaagcatcca cgaatcctgt ctgaaatacg gcaaagtcgg cgacacagta
     3121 aactacgtca atggtgcgaa cattgccggt ttcgtcaaag ttgccgatgc catgctggcg
     3181 caaggcttct aagcaaacgc cgccgctccg caaacaaaat gccgtccgaa ccgcaaatgc
     3241 tgttcagacg gcatttcatt atccgcccgt tcgaatcggg tgatactatt tgtatccaga
     3301 attgttggat agtttgggag tggagaggaa gaaatgatta tggaccaata ctatatggag
     3361 ttaaagaata agttatctaa cagaccaatc ctactggata atactaatga ttttcttttt
     3421 gttttagtaa atacagtgaa agctatgata gaaaatactg ataaaagtca gctttcagag
     3481 ttagataaaa ttttagacgg tgtcacaagt caagaattga aacttgccta tgatttttgt
     3541 caagggaaat tcggtcaagc aggtttttct tatcgtaggc acccgaatta tttttatttg
     3601 tccagtttaa ttgcgacttt tccagaattt gagttgtcta aagcagaccg agattacctc
     3661 aaaggaatca taaattttga taactacctt ttatatgagt tagattagtc tttaatcacc
     3721 tcaaaaccct atttattcaa aaggagaaaa aatgattgag ctgaaattag tagatgagag
     3781 cagttttcag gcagtgctgg atttgaaaat atctgaagct gatgaacgag cacgttttgt
     3841 ggctccaaat gtacgctctt tagctgacgc atggctctac cgggaaaatg aagatgtgtt
     3901 tccgagggca atctattggg ataagcaagt ggttggcttt ctcctgctgg aaatagacaa
     3961 ggatgaagca gaatacttta tctggcggat aatgattggt cagcagtg
//
LOCUS       DAXHPG010000171         3992 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXHPG010000171 DAXHPG010000000
VERSION     DAXHPG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.171"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(88..906)
                     /locus_tag="ACFM6F_001891"
     CDS             complement(88..906)
                     /locus_tag="ACFM6F_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6165253.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDIFRNFSL"
     gene            complement(986..2236)
                     /locus_tag="ACFM6F_001892"
     CDS             complement(986..2236)
                     /locus_tag="ACFM6F_001892"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6165254.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTTALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPHGMDADDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="ACFM6F_001893"
     CDS             complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="ACFM6F_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC6165255.1"
                     /translation="MFDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     SANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            2884..3696
                     /gene="murI"
                     /locus_tag="ACFM6F_001894"
     CDS             2884..3696
                     /gene="murI"
                     /locus_tag="ACFM6F_001894"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC6165256.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          897 a          987 c         1163 g          945 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggcgtg tccattttgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcggaaaa tatccatatc
      121 aggctgtggg ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggag aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatgcagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaaag gattttctat tttgggagct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttagataa aggggtgtag tctgtcccgc ttatggtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttcgg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggctcg gggctgtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactgt acacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gtccgttaaa acagataaag gaagccgatc ggacaattcg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacaa
      961 ctatattgaa acaccgcctt ttcaatcaac cccgcttcaa tacggatgta ttgatgtagc
     1021 gttggacacc cgaggcaatg gattgggcgc actgtcggcg gaaggattcg ctgcccagca
     1081 gcttctcttc ggcagggttg gacaggaagg cggtttcgac caggatagac ggcatatcgg
     1141 gtgcgcgcaa aacggcgaaa ttggcttcgt ccaccctgcc tttgtgcaga tggttgagcc
     1201 tgcccaattc ttcaagcacc agtttgccga gtttgcggct gtcgcgcagt gtggcggttt
     1261 gggtcatatc gagcagggcg gtatcgacgt tgcggttgcc gctggtcggt acgccgccga
     1321 ccgcgtcggc attgttttgc gtctgttcca agaatttggc ggcagagctg gtcgcgcctt
     1381 tggtgtttaa catataaacc cccgtgccgc gcgcggaggg gctggtgaag gcatcggcgt
     1441 ggatggagac aaatacgtcc gcccgccgtg cgcgcccttt ggcgacacgc acgcccaatg
     1501 ggatgaacac gtcttcgttg cgcgtcataa agacgttgta acccaaggct tccaattgtt
     1561 ttttggtttc gcgggcgatg gagaggacga cgtgtttttc ctgcaaaccg cccgggctga
     1621 cggcgccggg gtcttcgccg ccgtgtccgg ggtcgatcat aatgacgggt ctgcgcccgt
     1681 ttttgccgcg ttcggtttgt gcgggagtgt tttgagcgag gtcggcttca ggggagccgc
     1741 gacgggtttt gttcaggctg ccgttaagca gcgccatcat cggatcgtcg gcatccatcc
     1801 cgtgcggata gagatcgacg acgaggcggt ttttaaagcc gccgacgggg gggagggaaa
     1861 aaacttgcgc gtaggaaggc tgtttcaaat cgatgacgag gcggacggtg gtcggcgtgt
     1921 tctgacccgc gcgtatgctg cggataaagg ggtcgtctgc catgactttc tgagacagtc
     1981 cgtgcaatac ggtattgatg ttcgcgtttt gtatgtcgac gaccagcctg cccgggttgt
     2041 cgagcgtgaa gtgttggtat ttgagcgcgg ttgtgctttc cagcgtcagg cgggtgtagg
     2101 tgtgcgatgg ccatatccgt gcggcagtga attgtggtgc gcgtaccgtt ttggcaacgg
     2161 cggatgcgat ggggcttagg gcgaacagtg tgccggcggt gcggcggatg atttgtcttc
     2221 gtgtcagttt ggtcatagcg gcaggctttc gcgtcctcgt tcggtatggg cggtcagcag
     2281 gcattttctg ccgtcgccgt cgtgtgtcaa tgttgcggtg atgtcggcag gcggcgtaaa
     2341 ttcgcctccc tgttgcggcc attcgatcag gcagacgctg tttgcggaaa acagttcgtc
     2401 caatccggca tcttcccatt cttcggggaa cgagaagcgg tagaggtcga aatggtgcaa
     2461 ggtgaagcgt tccagcggat aagattcgac gatggcgtaa gtcgggcttt tgactgcgcc
     2521 ctgatgaccc aatccgcgca ggatgccgcg tgtcagcgtg gttttccccg cacccaaatc
     2581 cccttcgagg taaatgacca gcggtgcgtt taaacgggaa gaccacgccg cgcccaaatc
     2641 gagtgtggcg gcttcgtcgg caaggaatcg ggaaacgggg ggaaaatcaa acataggggc
     2701 tttacaactt ttatgtggcg gatcggaggg gcaggcggcc ggaaaaccgt gcgcccggcg
     2761 gtagcattgc cgtatattat aacagccaaa tccgcatcct gctttcagac ggcaacggct
     2821 gtcaagaaaa agcggcacgt gtacaatacg cagattgtat gtttaggacg gattggaaaa
     2881 aggatggaaa atatcggcag gcagcgaccc atcggcgttt ttgactcggg aatcggcggt
     2941 ttgaccaatg tgcgcgcgct gatggagcgg ctgccgatgg agaacatcat ttatttcggc
     3001 gatacggcgc gcgtgcctta cgggacgaaa tctaaggcga ccatcgaaaa tttctcgatg
     3061 cagattgttg attttttatt ggaacacgat gtcaaggcga tggttatcgc gtgcaacacg
     3121 attgcggcgg tggcggggca gaaaatccgt caaaagacgg gcaatatgcc ggttttggac
     3181 gtgatttccg ccggcgcgaa agccgcgctg gcaacgacgc gcaacaacaa aatcggcatt
     3241 atcgccacca atacgacagt caacagcaac gcctatgcgc gcgccatcca taggaacaac
     3301 cccgacacgc ttgtccgcac gcaggccgcg ccgctgctcg tccctttggt ggaagagggc
     3361 tggttggaac acgaagttac ccgcctgacc gtatgcgaat acctcaaacc attgcttgca
     3421 gacggcatcg atacgctggt gttgggctgc acgcactttc ccttgctcaa gcccttaatc
     3481 ggcagggagg cgcacaatgt cgcgttggtt gattctgcaa ttacgactgc cgaagaaacc
     3541 gcacgcgtcc ttgctcagga aggattgctc aataccgaca acaacaatcc cgactaccgt
     3601 ttttacgtca gcgatattcc tttgaaattc agaaccatcg gcgagcgttt tctgggcagg
     3661 acgatggagc agattgaaat ggtgtctttg gggtagcggc aggtacggac ggaaaccgtc
     3721 cccgtccgcg ccatccgcat acgcacctgc aatgccctgc cgtattaaac gccccgtctc
     3781 cgcaaatgcc gtctgaaagg cttcagacgg cattttttac acaactccca ccgtttccca
     3841 tccttctcga tacactgtaa tctcgaaacc cgtcattccc gcgcaggcgg gaatctagac
     3901 attcaatgct aaggcaattt atcgggaatg actgaaactc aaaaaactag attcccactt
     3961 tcgtgggaat gacgcgaaca gaaacctcaa at
//
LOCUS       DAXHPG010000172         3861 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXHPG010000172 DAXHPG010000000
VERSION     DAXHPG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.172"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="ACFM6F_001895"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="ACFM6F_001895"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC6165257.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="ACFM6F_001896"
     CDS             1439..1924
                     /locus_tag="ACFM6F_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC6165258.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFM6F_001897"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFM6F_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC6165259.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNELIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFGGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          783 a          944 c         1080 g         1054 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ccgtcagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataag
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgccga tgtcaaacct
      661 gaggacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggcgcg
      721 ataccgcgtt gggtgttgtt aagtgcggct ttgcccgaat tggatgaggt atggctggaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcattgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tatatttaat ccgtttcggt ttcgcgcctt cttcgccgag
     2041 tcggcgtttc ttatcggctt cgtattcctg atagttgccg ccgaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgcaaa atatcgcgac cttcggcgat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacgtt cacaaattca atcaattcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaaa tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgt gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgcgttacc gcaacgactg tgccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 cgattccgca tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gcttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc agttcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg cgataatcgc ttccaagcga ccttgttctt ccgccaatgc
     3301 gtcaaaatcc gcatcaggat tggcatactc ggcatacact tcttccaaac gtttttgtgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggttttct caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt gatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacgcccg ccataatccg caaaacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatacaga caaaaaaaat aataatgata ggggatcgcc gccccggcaa
     3841 acatttcgga ttttccaaag c
//
LOCUS       DAXHPG010000173         3844 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXHPG010000173 DAXHPG010000000
VERSION     DAXHPG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.173"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..2076
                     /locus_tag="ACFM6F_001898"
     CDS             <1..2076
                     /locus_tag="ACFM6F_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC6165260.1"
                     /translation="GKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFGG
                     ESNTLTGLYFRGYQLDARQFSVNGLAGMYGTQGTASVQVGSAQLIKGASTAVNGMNPE
                     GAVSGSVNIETKKAADEGNRKIGLGRFSNNRAQGTFDLGQRFGENKAFGVRANGKLRH
                     GDTPRHGYSEDNKEFAVNADYRGETLRVAFDSIYAKRKTNGGRARMQDIQNAGGRLFG
                     APDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVCL
                     NATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLSARGEFETGPVTHN
                     WSTAFDRVIRQRKTINGSGNGNSKIEVKANENIANQLASFTADYPNSWENSANLDANI
                     KVNSLALSDTLGFVDNKYRLTLGERFQTVEYTDKKSQSGDAKRFSPMLMAAWVPQPDL
                     VVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGDFVTTLNAFQIKRPG
                     YWRGNTKKGTDFAAYKAAGGADGDEQGMERSRGIEFNAYANLLNKTLRPTFGLMYLQS
                     TVKDYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLNSNVSYFGKSYQDTQKQ
                     YAFPSYTLVDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQRGQYDRSFAVVGLPRT
                     YWLKAELDF"
     gene            2200..3750
                     /locus_tag="ACFM6F_001899"
     CDS             2200..3750
                     /locus_tag="ACFM6F_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC6165261.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNAQKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGNEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         1087 a          951 c          968 g          838 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcgccgatt accgtcgtca actacgacga acaagccctc
       61 aacaacaccg aagcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gagaaagcaa cacgctgact ggactgtatt tcagaggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggtttggca ggtatgtacg gcacgcaagg cacggccagc
      241 gtgcaagtcg gctccgcaca actgattaaa ggcgcgtcca ccgccgtaaa cggcatgaac
      301 cctgaaggtg cggtatccgg ttccgtcaat atcgaaacca aaaaagccgc cgatgaaggc
      361 aaccgcaaaa tcggtttggg ccggtttagc aacaaccgcg cccaaggtac gttcgatttg
      421 ggtcaacgct tcggcgaaaa caaagcattc ggcgtacgcg ccaacggcaa actgcgccac
      481 ggcgacactc cgcgccacgg ttacagcgaa gacaacaaag aatttgcggt aaatgccgac
      541 tatcgcggcg aaacactgcg cgtagcgttc gattccatct acgcaaaacg caaaaccaac
      601 ggcggtcgcg cgcgtatgca ggacattcaa aacgccggcg gacgcttgtt tggcgcgccc
      661 gatggtaaaa ccaacctgct gccttcttgg aactggcaaa ataccgccgg ccaaaccaat
      721 atgcttacgt ttgaatggga tgcgtttgat aatgcacaga ttacaggcgg tatcggctac
      781 aacaaggcac gctattacgg cacgctgatt tccccgaccg tatgtttgaa tgctaccagc
      841 acttgtaccg atatgcaaaa cacaaacgcc agcggcaaag taacggcacg caactatgac
      901 tacaataccg gtactgcgcg cctgaccgac caatacttcc gcactttaag catgaacctg
      961 tctgcgcgag gcgaatttga aaccggcccg gtaacacata actggagtac ggctttcgac
     1021 cgcgttatcc gccagcggaa aaccataaac ggcagcggga acggcaacag caagatagaa
     1081 gtgaaggcaa acgaaaatat tgcgaatcaa ttggcatcgt ttacagccga ctatcccaat
     1141 tcttgggaga actctgccaa cttggatgcc aatatcaaag tcaacagcct ggctttgtcc
     1201 gacaccttgg gcttcgtgga taacaaatac cgcctgactt tgggcgaacg tttccaaaca
     1261 gtcgaatata ccgacaaaaa atcacagagc ggcgatgcca aacgtttcag cccgatgctg
     1321 atggcggcgt gggtgccgca acctgatttg gttgtgtacg gcaactatat ggaagacttg
     1381 gagcctgccg acattaaaac cgatgattcc ggtgaaacga caatggcgaa accgcgcgtc
     1441 agccgccagt ttgaagtggg cgttcgtaaa aactggggcg actttgttac gacgttgaac
     1501 gcgtttcaaa tcaaacgccc gggctactgg cgcggcaata ccaaaaaagg tactgatttt
     1561 gcagcatata aagcagcagg aggcgcagac ggagatgaac aaggcatgga acgcagccgc
     1621 ggtatcgaat tcaatgccta tgccaacctg ttaaacaaaa ccctgcgccc gactttcggc
     1681 ctgatgtacc tgcaatcgac cgtgaaagac tacccaaatt cacgcgatat gctggttaac
     1741 ggcgtacaag tcgccaatcc gcgcgtaatt gccaaggcag gtttggaatg ggatacaccg
     1801 tttgccaaag gcttgacttt aaacagcaat gtttcgtatt tcggcaagtc ttaccaagac
     1861 acgcaaaaac aatacgcctt cccatcctat accttggttg atgtaggcgc gcgctacaaa
     1921 accaagctcg gcaaaaatac gttgaccgtc agcagctcgg tggaaaatct gttcaacaaa
     1981 aactactggc aggtacagcg cggccaatac gaccgcagct tcgccgtcgt cggtctgccg
     2041 cgtacttact ggctgaaggc ggaattggat ttctgatttc tccgcatctg gaaaatgccg
     2101 tccgaacagg tttcagacgg catttctttt aaagagatat ggcgggatac gcttaaataa
     2161 tgctattcta gcgtctttga aacctgcttg tttgaagtga tgaaaaaacc tttgatttcg
     2221 gttgcggcag cattgctcgg cgttgctttg ggcacgcctt attatttggg tgtcaaagcc
     2281 gaagaaagct tgacgcagca gcaaaaaata ttgcaggaaa cgggcttctt gaccgtcgaa
     2341 tcgcaccaat atgagcgcgg ctggtttacc tctatggaaa cgacggtcat ccgtctgaaa
     2401 cccgagttgc tgaataatgc ccaaaaatac ctgccggata acctgaaaac agtgttggaa
     2461 cagccggtta cgctggttaa ccatatcacg cacggccctt ttgccggcgg atttggcacg
     2521 caggcgcaca ttgaaaccga gttcaaatac gcgcctgaaa cggaaaaagt tttggaacgc
     2581 ttttttggga aacaagctcc ggtttccctt gccaataccg tttattttaa cggcagcggt
     2641 aaaatggaag tcagtgttcc cgccttcgat tatgaagagc tgtcgggcat caggctgcat
     2701 tgggaaggat tgacgggaaa aacggtttat caaaaaggtt tcaaaagcta ccgcaacagc
     2761 tatgatgcgc ccttgttcaa aatcaagctg gcagacaaag gcgatgccgc gtttgaaaaa
     2821 gcgcatttcg attcggaaac ttcagacggc atcaatccgc ttgctttggg cagcagcaat
     2881 ctgaccttgg aaaaattctc cctagaatgg aaagagggtg tcgattacaa cgtcaagtta
     2941 aacgaactgg tcaatcttgt taccgatttg cagattggcg cgtttatcaa tcccaacggc
     3001 agcatcgcac cttccaaaat cgaagtcggc aaactggctt tttcaaccaa gaccggggaa
     3061 tcaggctcat ttatcaacag tgaagggcag ttccgttttg acacgctggt gtacggcaat
     3121 gaaaaatacg gcccgctgga catccatatc gccgccgaac atctcgatgc ttctgcctta
     3181 accgtattga aacgtaagtt tgcacaaatt tccgccaaaa aaatgaccga ggaacaaatc
     3241 cgcaatgatt tgattgccgc cgtcaaaggc gaggcttccg gactgttcac caacaatccc
     3301 gtattggaca ttaaaacttt tcgtttcaca cagccgtcgg gaaaaatcga tgtgggtgga
     3361 aaaatcatgt ttaaagacat gaagaaggaa gatttgaacc aattgggttt gatgctgaag
     3421 aaaaccgaag ccgacatcag aatgagtatt ccccaaaaaa tgctggaaga cttggcggtc
     3481 agtcaggcag gcaatatttt cagcgtcaat gccgaagatg aggcggaagg cagggcaagt
     3541 cttgacgaca tcaacgagac cttgcgcctg atggtggaca gtacggttca gagtatggca
     3601 agggaaaaat atctgacttt gaacggcgac cagattgata ctgccatttc tctgaaaaac
     3661 aatcagttga aattgaacgg taaaacgttg caaaacgaac cggagccgga ttttgatgaa
     3721 ggcggtatgg tttcagagcc gcagcagtaa tgggtcaaat gccgtctgaa atgttcaggc
     3781 ggcattttgt tgtattggaa actgccgttt tcaaccattc ctttagataa gccgtgtcgg
     3841 attc
//
LOCUS       DAXHPG010000174         3758 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXHPG010000174 DAXHPG010000000
VERSION     DAXHPG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.174"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..294)
                     /locus_tag="ACFM6F_001900"
     CDS             complement(<1..294)
                     /locus_tag="ACFM6F_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HFC6165262.1"
                     /translation="MFLILGRNFLKIILCFSFFVPKFALASVNVPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVS"
     gene            complement(641..943)
                     /locus_tag="ACFM6F_001901"
     CDS             complement(641..943)
                     /locus_tag="ACFM6F_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165263.1"
                     /translation="MGYRVGMNCFDTRLQADDYLLSSLPPTVTEEGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIFGFRLLINFLKDMGKVGTD"
     gene            complement(971..1201)
                     /locus_tag="ACFM6F_001902"
     CDS             complement(971..1201)
                     /locus_tag="ACFM6F_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165264.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDAAAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            complement(1439..1639)
                     /locus_tag="ACFM6F_001903"
     CDS             complement(1439..1639)
                     /locus_tag="ACFM6F_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165265.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEMGGGFQIFQFFVKSE"
     gene            complement(1643..1954)
                     /locus_tag="ACFM6F_001904"
     CDS             complement(1643..1954)
                     /locus_tag="ACFM6F_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165266.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSRLENLKYPCEVMVMVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            complement(1966..3180)
                     /locus_tag="ACFM6F_001905"
     CDS             complement(1966..3180)
                     /locus_tag="ACFM6F_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC6165267.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTP
                     DQALLDHDNGFFDNSNQRPKSETIGTAWRNEDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKT
                     LNLTFEHKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDVEKERKYQEYLS
                     KVYHQNVDYDYF"
BASE COUNT         1190 a          876 c          560 g         1132 t
ORIGIN      
        1 agatacaacc gaactaactt tacccgtact tgatgctcca gtagatacat gcctaacttt
       61 taaatcttga gcttctgaag ttaaatatct tcccgattct ctatcaaata cacctttcca
      121 aattcttctt cttttgtcat ctgaacctcg aatacctaaa tatctgccat catcataaac
      181 ttcaacccta tcaaatttac ccggaacatt tactgatgcc aatgcaaatt taggtacaaa
      241 aaaactaaag cataggataa tctttaaaaa atttcgccct aggataagaa acataacatt
      301 ttgcttcatc taatttaatt aataatgtag aagaatcagg gaattgaatt tctataaaat
      361 ctctatcata acgatgaaag aaattcctaa aaatatcaaa tgaaaacgaa gttttaaaaa
      421 ccccttttct aatcaattcc ctataataaa ttaacgcttg tggtaaatct ctataaatat
      481 gtgaatcagt ttgaaattta actaaatgat attcagaaat aaacataatt cggacctttt
      541 aaaacactct aaaaagccga ttctaccaac cgttaccaaa acagccaagc aacagacaaa
      601 gccaagaaga aaccgagaag aaaccaaaaa tcaataatca tcaatcagta ccaaccttgc
      661 ccatgtcttt taagaaatta atcagaagcc tgaaaccgaa aatgactacg aacagaatta
      721 gaaccgtcga accgacataa gaaccttgct ttatctgctc aaaattggaa cattccggat
      781 aagacaacgt aaccggcttt ccgttcaaaa tccatttatc gcccaccctt tccggcctga
      841 tgatttttcc ttcttctgta acagtaggag gaagagacga caataaatag tcgtctgcct
      901 gcaatcttgt atcaaaacag ttcatgccga cacgataacc cattcacgcg cccctctttc
      961 ttcactgccg ttatttgaca gatttaatca tgctccaagc cattttgaag ccttggattg
     1021 ccaaaatgac ggtaatggcc gccataccca cggcggaaac catcgcaacg aaacccatga
     1081 ttacattcgc cacttgcgta ccaatcgcgg ctgcatcaaa gccatctgcc ataacaatag
     1141 ccggtgtgaa gataccagct gccaacaatg cttttacagc atatttttta acgatgttca
     1201 tcgttttttt cctttttttg atatttaaaa taagacgact tcttgacttg cttcatccgg
     1261 acgaagtctt ttccgaatct cgtttttagc cgataaaata gaggattgcg aaaagaaaaa
     1321 gaaacataca gaccacccag ccgataatta gtgttgcaag gttcattttc atgatatttt
     1381 tcctttgttg cgggctttgt gaaaggttaa cagaccgccc gccgagcctg tttttctttt
     1441 attccgattt tacgaagaac tgaaatatct ggaatcctcc gcccatttca tttatgcctg
     1501 aattcaacgc atcttcgtaa ctttcaaatt gacctgctga tttaatattt tgagtaaacc
     1561 ccacatcacc gaacggatcg ggataaataa agtcatgcgt ttctaaatct tgaactatga
     1621 aacgttcttc aaatttcata aatcaacctt tcggcttttc cgccacctga aaatcaatta
     1681 atgacggaac catgccttta cctgtcgaag tcatttcaac cattaccata acttcgcacg
     1741 gatatttgag attctctaac cttgagaaat tcttactatc cccgaacttc atttgtgctg
     1801 ccgtaaatcc aacagcatta cccgactgtg ccggtaaagg tgttgcaacc aatacggaac
     1861 aagtgtcaat gttagagcca tcaatttcac ctttgaattt tttggctccc aaaaaagttg
     1921 caggataagt tacagtttga gtttgattaa acattttgat ttcctttaaa aataatcata
     1981 atctacattt tgatgataaa ctttacttaa atattcctga tatttccttt ctttctcgac
     2041 atcataatca ggactaaaaa gccttttttc cctatcgaat ttgtcagaat ttttaaaagc
     2101 aataaccccc aattcatcaa gttcaatttc caaatcaata tccggctgtt catgaataaa
     2161 accgtgtttc aaaccgtccc ttaacatttc cagagcatat ttttcaggtt ctaatccttt
     2221 gggaaatccc gaatctgcct ttaaagattc cacaatttcg ctattatcaa aacccatttc
     2281 aatcatgaaa ttgaccagtt ttccaaccgc gtttttcgcg taatgcaatt tatgctcgaa
     2341 agttaaatta agcgttttct ttctctgatc aaacctttcg ggaaccggca tatttttaaa
     2401 ttttctacaa attggaaaag ctccacagaa atacgaaccc tgatttatta aaatatccaa
     2461 gggtatttct atatctccat aattaaactg gatctcgaac cttacccatt tgctttcttt
     2521 atctccaagc tgcctgcctt tctcataaac acgaacaaaa cgagaatttt tcttgcgacc
     2581 tacataaaat gtcttgccgc tcccgtcctc attccgccaa gccgtaccga tcgtttcaga
     2641 tttcggcctt tgattgctgt tatcaaaaaa accattatcg tgatctaaca acgcctgatc
     2701 cggcgtgtac tctccatcaa aaaaatcaag tgctaggtca attcgcgtta ttcttgtcct
     2761 tatcgaatca tcgagaaact gctttagcct caactcccaa cccggacttg caacgctgca
     2821 accagtacct ttcaactcaa ctaaaacagt attgcgctga cctccgaaat gcacctctcc
     2881 ataatcaaca tcatccgaac ctaacctata catggattca tagaatttgt tgccccttga
     2941 tttgcatttg cgcgttatgc caaaacctag aatttcttcc agctttctgc ttaatacata
     3001 catgtattca gcatcagaaa ataaagggca accggaaact ttcagtaaag tatcttcatg
     3061 gaatgtgaat gaaatccaat caacaaaaac cccgtcttgc ttacctcttc gctgcggaat
     3121 ttctaaaagc ttcccttttc cgtccgatat aaagtgtgag aaatattcaa cttcactcat
     3181 tcgttcacta ctcttggaat tggtttttaa tttcgctccc cccctgttag tcaagggggg
     3241 gctttcagcc gtttcccgtc ttccgcgcta aagcgcgtac gacggtcaac gaccgaaagc
     3301 caaatcctga caaactttta aagatcaaca aaaaagacca caaccatccg ttgtgataat
     3361 cactggaaaa ttcgaaccaa ccgaatctat ataatcgaac gcctgataaa gctttgaaaa
     3421 attctcttga ttaatgagtt tatgcggttc gccatgtctg aactgataaa aacacaaaac
     3481 acaagaatct gattttttaa atattctcca ataagaacag gaaaatatta catttactgc
     3541 tgacataaaa aagccccttt cacttggctg tcaaagggga atgttaagaa aattaatgcg
     3601 cccctttgat agagcgcatc atataaggcg ggaatctaga ccgttcggtt tcggtttttt
     3661 tggttagtgc cgcaacatta aatttctaga ttcccacttt cgtgggaatg acggcggagc
     3721 ggtttctgct ttttccaata aatgacccca acctaaaa
//
LOCUS       DAXHPG010000175         3723 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXHPG010000175 DAXHPG010000000
VERSION     DAXHPG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.175"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            346..3543
                     /gene="nalP"
                     /locus_tag="ACFM6F_001906"
     CDS             346..3543
                     /gene="nalP"
                     /locus_tag="ACFM6F_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HFC6165268.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGTSAPDFNAGGTG
                     IGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLHTGD
                     FTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNENY
                     KNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHIIGGR
                     SVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGTTSR
                     AGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFIFS
                     ASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAPYE
                     ASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAVGV
                     DSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGSQL
                     QLHGNNTYTGKTVIEGGSLVLYGNNESDMRVETKGALIYNGAASGGSLNSDGIVYLAD
                     TDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAIIGGKLYMSARGKGAGYLNR
                     TGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNAAR
                     TASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGIRP
                     YGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDHNAT
                     GLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTWSENS
                     ANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLMQL
                     GALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAGLK
                     LSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGLGA
                     DVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1005 a         1015 c          990 g          713 t
ORIGIN      
        1 gaaccgattc acttgttaaa gaatcgttct ctttgagcta aggcgaggca acgccgtact
       61 ggtttttgtt catccactat aactagggaa attcaaatta agttagaatt atccctataa
      121 gaaaaagccg tctaagccgg tataaacaaa ataaactcat tgagctattt gtcgaaagtt
      181 caaatttcca ttttaaaaca attagtaaaa tcgagtttat cctagttgtc caagacaacc
      241 cctataataa tataattcaa aatataaaaa tgggttacat ctaaacatta cggaattttt
      301 attccctcgc ctgaattcta ttgtcagatt caaggagacc tcatcatgcg aacgacccca
      361 accttcccta caaaaacttt caaaccggct gccatggcgt tagctgttgc aacaacactt
      421 tctgcctgct taggcggcgg cacttctgcg cccgacttca atgcaggcgg caccggtatc
      481 ggcagcaaca gcagggcaac aacagcgaaa tcagcagcag tatcttacgc cggtatcaag
      541 aacgaaatgt gcaaagacag aagcatgctc tgtgccggtc gggatgacgt tgcggttaca
      601 gacagggatg ccaaaatcaa tgcccccccc ccgaatctgc ataccggaga ctttacaaac
      661 ccaaatgacg catacaagaa tttgatcaac ctcaaacctg caattgaagc aggctataca
      721 ggacgcgggg tagaggtagg tatcgtcgat acaggcgaat ccgtcggcag catatccttt
      781 cccgaactgt atggcagaaa agaacacggc tataacgaaa attacaaaaa ctatacggcg
      841 tatatgcgga aggaagcgcc tgaagacgga ggcggtaaag acattgaagc ttctttcgac
      901 gatgaggccg ttatagagac tgaagcaaag ccgacggata tccgccacgt aaaagaaatc
      961 ggacacatcg atgtggtctc ccatattatt ggcgggcgtt ccgtggacgg cagacctgca
     1021 ggcggtattg cgcccgatgc gacgctacac ataatgaata cgcatgatgg aaccaagaac
     1081 gaaataatgt ctgcagccat ccgcaatgca tgggtcaagc tgggcgaacg tggcgtgcgc
     1141 atcgtcaata acagttttgg aacaacatcg agggcaggca ctgccgacca tttccaaata
     1201 gccaattcgg aggagcagta ccgccaagcg ttgctcgcct attccggcgg tgataaaaca
     1261 gacgagggta tccgcctgat gcaacagagc gattacggca acttgtccta ccacatccgt
     1321 aataaaaaca tgcttttcat tttttcggca agcaatgacg cacaagctca gcccaacaca
     1381 ctgaccctat tgccatttta tgaaaaagac gctcaaaaag gcattatcac agtcgcaggc
     1441 gtagaccgca gtggagaaaa gttcaatggc tccaaccatt gcggaattac tgccatgtgg
     1501 tgcctatcgg caccctatga agcaagcgtc cgtttcaccc gtacaaaccc gattcaaatt
     1561 gccggaacat ccttttccgc acccatcgta accggcacgg cggctctgct gctgcagaaa
     1621 tacccgtgga tgagcaacga caacctgcgt accacgttgc tgacgacggc tcaggacatc
     1681 ggtgcagtcg gcgtggacag caagttcggc tggggactgc tggatgcggg taaggccatg
     1741 aacggacccg cgtcctttcc gttcggcgac tttaccgccg atacgaaagg tacatccgat
     1801 attgcctact ccttccgtaa cgacatttca ggcacgggcg gcctgatcaa aaaaggcggc
     1861 agccaactgc aactgcacgg caacaacacc tatacgggca aaaccgttat cgaaggcggt
     1921 tcgctggtgt tgtacggcaa caacgaatcg gatatgcgcg tcgaaaccaa aggtgcgctg
     1981 atttataacg gggcggcatc cggcggcagc ctgaacagcg acggcattgt ctatctggca
     2041 gataccgacc gatccggcgc aaacgaaacc gtgcacatca aaggcgatct gcagctgggc
     2101 ggcgaaggta cgctgtacac acgtttgggc aaactgctga aagtggacgg tacggcgatt
     2161 atcggcggca agctgtacat gtcggcacgc ggcaaagggg caggctatct caaccgtacc
     2221 ggacaacgtg ttcccttcct gagtgccgcc aaaatcgggc gggattattc tttcttcaca
     2281 aacatcgaaa ccgacggcgg tctgctggct tccctcgaca gcgtcgaaaa aacagcgggc
     2341 agtgaaggcg acacgctgtc ctattatgtc cgtcgcggca atgcggcacg gactgcttcg
     2401 gcagcggcac attccgcgcc cgccggtctg aaacacgccg tagaacaggg cggcagcaat
     2461 ctggaaaacc tgatggtcga actggatgcc tccgaatcat ccgcaacacc cgagacggtt
     2521 gaaactgcgg ccgccgaccg cacagatatg ccgggcatcc gcccctacgg cgcaactttc
     2581 cgcgcagcgg cagccgtaca gcatgcgaat gccgccgacg gtgtacgcat cttcaacagt
     2641 ctcgccgcta ccgtctatgc cgacagtacc gccgcccatg ccgatatgca gggacgccgg
     2701 ctgaaagccg tatcggacgg gttggaccac aacgctacgg gtctgcgcgt catcgcgcaa
     2761 acccaacagg acggtggaac gtgggaacag ggcggtgttg aaggcaaaat gcgcggcagt
     2821 acccaaaccg tcggcattgc cgcgaaaacc ggcgaaaata cgacagcagc cgccacactg
     2881 ggcatgggac acagcacatg gagcgaaaac agtgcaaatg caaaaaccga cagcattagt
     2941 ctgtttgcag gcatacggca cgatgcgggc gatatcggct atctcaaagg cctgttctcc
     3001 tacggacgct acaaaaacag catcagccgc agcaccggtg cggacgaaca tgcggaaggc
     3061 agcgtcaacg gcacgctgat gcagctgggc gcactgggcg gtgtcaacgt tccgtttgcc
     3121 gcaacgggag atttgacggt cgaaggcggt ctgcgctacg acctgctcaa acaggatgca
     3181 ttcgccgaaa aaggcagtgc tttgggctgg agcggcaaca gcctcactga aggcacactg
     3241 gtcggactcg cgggtctgaa gctgtcgcaa cccttgagcg ataaagccgt cctgtttgca
     3301 acggcgggcg tggaacgcga cctgaacgga cgcgactaca cggtaacggg cggctttacc
     3361 ggcgcgactg cagcaaccgg caagacgggg gcacgcaata tgccgcacac ccgcctggtt
     3421 gccggtctgg gcgcggatgt cgaattcggc aacggctgga acggcttggc acgttacagc
     3481 tacgccggtt ccaaacagta cggcaaccac agcggacgag tcggcgtagg ctaccggttc
     3541 tgacggacag gaagcagaca gccgcaaaga tcacggtctt tgcggctgtt tcttatgaaa
     3601 agaaaaccct attccaattg cctgcttcta ttgtttcaag acttcttcca aagattcggc
     3661 atcaatcaga tgtatagcgg attaacaaaa atcaggacaa ggcggcgaag ccgcagacag
     3721 tac
//
LOCUS       DAXHPG010000176         3617 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXHPG010000176 DAXHPG010000000
VERSION     DAXHPG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.176"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            135..493
                     /locus_tag="ACFM6F_001907"
                     /pseudo
     CDS             135..493
                     /locus_tag="ACFM6F_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            524..3376
                     /gene="gcvP"
                     /locus_tag="ACFM6F_001908"
     CDS             524..3376
                     /gene="gcvP"
                     /locus_tag="ACFM6F_001908"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC6165269.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVTLRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVGDFAQAEEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGSK
                     EQADQVFAAALESGYNLRRVSDTQVAAAFHETSTREDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPETQTAGYRELLTDMENSLKAITGFDAISLQPNSGAQGE
                     YTGMLSIRRYQEANGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDTLSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHVVTDMHNASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVERGEWPKEDNPLVNAPHTASDVTGEWTHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
BASE COUNT          861 a         1042 c          927 g          787 t
ORIGIN      
        1 catgaaccga ctggaacacc aaactgcttc gggacgcata tgccgtctga agtgcaaaac
       61 ctacgccata cagccgcatg aagttgcagc ggtatggcgt tttttgaaaa agacggcctg
      121 ccggttcaga cggcatgacc gaccgtccga gcctgctgtg gataaagccc ggacaggctg
      181 aaatcatgga atattgcgaa cctgaagaag catccgaccc gtacgcaaca tacaggcgtg
      241 ccaacctgat ggcgaggctg ccgctgtttg tcgtgatttt ggttctgctc aatattgttt
      301 tccgcttccg gcgcatccct tagcttggct ggtgcctgca ggtttcatgg ttttggtcgg
      361 cggctttccc ttatcgctgc cgcttgtggc gctgcttgtc ctgacctgct gcattctggc
      421 gcattgtccg ccattatccc gtcttttgtg ctacccttgc ccgaatcatc cgatgtctaa
      481 aaattctgcc tgatggcagc cctacaaacc cgaaggagta gaaatgaaac tgtccgaact
      541 gttcaacccc gacgaatttg ccgcgcggca tttgagtttc ggcgacgaag cggcgttgct
      601 tgccgctgtc ggcgagaaaa gtatggacga ttttgtcggc aacaccgtgc cgcaaagcat
      661 ccgtatgccg tccgaactcg atttgcccga tgccctgacc gaagcggacg cgttggcaaa
      721 attgaaaggc attgcgtcga aaaacgtgat caacaagtcc tatatcggtt taggctatta
      781 cccgacccgc gtgccgaatg tgactttgcg caacgtattg gaaaatccgg gctggtatac
      841 cgcctacacg ccgtatcagg cggaaatcgc gcagggtcgt ttggaagcat tgttgaactt
      901 ccaacaagtg tgcatcgatt tgaccggttt tcccgtggcg ggcgcgtctt tgctggacga
      961 ggcaactgcc gcggctgaag cgatggcgat ggcgcaccga gtgggcaaag tgaaatccga
     1021 acgtttcttt gtggatgcgc gcgtgtatcc gcaaactttg gacgtgatga aaacccgcgc
     1081 caaatatttc ggctttgaat tggtggtcgg tgattttgcc caagcggagg aaggcgaata
     1141 cttcggtgcg ctgttccaat acgtcggcaa agacggcgat gtgcaagact tgcaggacgt
     1201 tatcggccgt ctgaaagcca aaggcacaat cgttgccgtt gcagccgaca ttatgagctt
     1261 ggttttgctg aagtctccgg ctgaactagg tgcggatatt gctttgggca acacgcaacg
     1321 cttcggcgtg cctatgggct ttggtggtcc gcacgccgct tatttcgcgt ttaaagacga
     1381 gttcaaacgt tccgccccgg gccgcatcat cggcgtatcc aaagacgcat caggcaaacc
     1441 tgccttgcgc atggcgttgt ccacccgcga gcaacacatc cgccgcgaaa aagcgacatc
     1501 caatatttgt accgcgcagg cattgctggc gaacttggcg ggtatgtacg ccgtttacca
     1561 cggccctgaa ggtgtgaaac gcatcgccaa ccgcattcac gcgctggctt ctgcctttgc
     1621 cgatgcgctg gtttcagacg gcctgaatgt ggttcataag gtcttcttcg ataccgttac
     1681 cgtcgatttc ggcagcaaag agcaagcaga ccaagtgttt gccgctgctt tggaatcggg
     1741 ctacaacctg cgccgcgtca gcgatactca agttgcggca gccttccatg aaacttcgac
     1801 acgcgaagat ttggtcgatt tgtaccgcgc gtttaccggc aaggatacgt tcgcatttgc
     1861 cgatgatgtc aaaggccgtc tgaatgccga attactgcgt caagacgaca ttctgcaaca
     1921 tcccgtgttc aaccgttacc acaccgaaca cgaaatgttg cgctatctga aaaaactcga
     1981 agaccgcgac ttggcgatga accgcagtat gatttcgctg ggcagttgca ccatgaaact
     2041 caatgccact gcggaaatgt tgccgattac ttgggcggag ttctccgaca tccaccccta
     2101 cgcccccgaa acccaaaccg caggctaccg cgaactctta acagacatgg aaaacagcct
     2161 gaaagccatc accggctttg acgcgatttc cttgcaaccc aactccggcg cacaaggtga
     2221 atacaccggt atgctctcca tccgccgtta tcaagaagcc aacggcgagg gacaccgcaa
     2281 catctgtttg attcccaaat ccgcccacgg taccaaccct gcgactgcgg ccatgctggg
     2341 cttgaaagtc gtcgttgtcg ataccgacga acacggcaac gtcaacatcg acgatttgaa
     2401 agccaaagcc gagcaacacc gcgacacttt gtccgccatc atgattacct atccgtctac
     2461 ccacggcgtg tacgaagaag gcatccgcga catctgccgc atcatccatg aaaacggcgg
     2521 acaggtttac atggacggcg ccaacctcaa cgcccaaatc ggcatcatgc agcctgccga
     2581 agtcggcgcg gacgtgttgc acatgaacct gcacaaaacc ttctgtatcc ctcacggcgg
     2641 cggcggtccg ggcatgggtc cgattggctt gaaagcccat ctggctccgt tcgcaccagg
     2701 ccatgtggta accgatatgc acaacgccag tgccgatcaa actgccgtgg ctgccgcagc
     2761 tttcggttct gcgtccatcc tgccgattac ttggatgtac ctgaccatga tgggcaaaca
     2821 aggcatggag caggcaacac gctgggcatt gctcaacgcc aactacgttg ccaaacgctt
     2881 gagcgaagac tatccgattc tgtatacagg caaaaacggc cgcgtcgcgc acgaatgtat
     2941 cgtcgacttg cgtccgctca aagccgaaag cggcattacc gaaaccgaca tcgccaaacg
     3001 cctgatggac tacggcttcc acgccccgac cgtctccttc cccgttgccg gcacgctgat
     3061 gatcgagccg accgaaagcg agagcaaagc cgaactcgac cgcttcatcg ccgccctgaa
     3121 acaaatcaaa caggaagtgt tgaaagtcga gcgcggcgaa tggccgaaag aggacaaccc
     3181 gttggtgaac gctccgcata ccgcatccga cgtaactggc gaatggacgc atccgtattc
     3241 ccgcgaagaa gccgtcttcc cattgccgtt cgtccgcgaa cacaaattct ggccgagcgt
     3301 caaccgtgtg gacgacgtgt acggcgaccg caatctcgtg tgcagctgcc caccgatgga
     3361 aaattatgaa gactgaccgt tgaaaccccg aagacaaatg ccgtctgaaa cattttcaga
     3421 cggcattttc atcaacggca aaccagttgc accgatacac gtatctcgac tataactcta
     3481 aaacaaatgg gttaaaccag tatccataca tcagcttttt tatcatccta ctttttattc
     3541 atccgatcgt gcaaacagat ttcaaagatg aaaagcctat tcacaccctt tgatgtcatt
     3601 tccacacgga caaacaa
//
LOCUS       DAXHPG010000177         3546 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXHPG010000177 DAXHPG010000000
VERSION     DAXHPG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.177"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            100..1215
                     /gene="lst"
                     /locus_tag="ACFM6F_001909"
     CDS             100..1215
                     /gene="lst"
                     /locus_tag="ACFM6F_001909"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC6165270.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKEKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLDKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAVKDFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            complement(1380..1838)
                     /locus_tag="ACFM6F_001910"
     CDS             complement(1380..1838)
                     /locus_tag="ACFM6F_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6165271.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFADSSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            1957..2778
                     /locus_tag="ACFM6F_001911"
     CDS             1957..2778
                     /locus_tag="ACFM6F_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6165272.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPNMVARGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            complement(2829..3275)
                     /gene="yciA"
                     /locus_tag="ACFM6F_001912"
     CDS             complement(2829..3275)
                     /gene="yciA"
                     /locus_tag="ACFM6F_001912"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC6165273.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIAQGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPILAGLLPTP"
BASE COUNT          832 a          763 c         1027 g          924 t
ORIGIN      
        1 tattcggtaa ctgtcggaat atctgctaaa attccgcatt tttccgtccc gggacactcg
       61 gggcgtatgt tcaatttatc ggaatggagt tttagggata tgggcttgaa aaaggcttgt
      121 ttgaccgtgt tgtgcctgat tgttttttgt ttcgggatat tttatacatt tgatcgggta
      181 aatcaggggg aaaggaatgc ggtttccctg ctgaaggaga aacttttcaa tgaagagggg
      241 gaaccggtca atctgatttt ctgctatacc atattgcaga tgaaggtggc agaaaggatt
      301 atggcgcagc atccggggga gcggttttat gtggtgctga tgtctgaaaa caggaatgaa
      361 aaatacgatt attatttcaa tcagataaag gataaggcgg agcgggcgta ttttttctac
      421 ttgccctacg gtttggacaa atcgtttaat ttcattccga cgatggcgga gctgaaggtg
      481 aagtcgatgc tgctgccgaa ggtcaagcgg atttatttgg caagtttgga aaaagtcagc
      541 attgccgcct ttttgagcac ttacccggat gcggaaatca aaacctttga cgacgggaca
      601 ggcaatttaa ttcaaagcag cagctatttg ggcgatgagt tttctgtaaa cgggacgatc
      661 aagcggaatt ttgcccggat gatgatcgga gattggagca tcgccaaaac ccgtaatgct
      721 tccgacgagc attacacgat attcaagggt ttgaaaaaca ttatggacga cggccgccgc
      781 aagatgactt acctgccgct gttcgatgcg tccgaactaa aggcggggga tgaaacgggc
      841 ggcacggtgc ggatactttt gggttcgccc gacaaagaaa tgaaggaaat ttcggaaaag
      901 gcggtaaaag atttcaacat acaatatgtc gcgccgcacc cccgccaaac ctacgggctt
      961 tccggcgtaa ccacattaaa ttcgccctat gtcatcgaag actatatttt gcgcgagatt
     1021 aagaaaaacc cgcatacgag gtatgaaatt tatacctttt tcagcggcgc ggcgttgacg
     1081 atgaaggatt ttcccaatgt gcacgtttac gcattgaaac cggcttccct tccggaagat
     1141 tattggctca agccggtgta tgccctgttt acccaatccg gcatcccgat tttgacattt
     1201 gacgataaag attaatcgca tagcaaatca aaatagaaaa tggcggagtg cgtggggtaa
     1261 aaataaggat agcgtttttt catttggatt gacgataatt tctgattgct ttgcgtgtgc
     1321 tgaaatggca aagaaaatgc cgtctgaagt cttcagacgg cattgttttg ttttggatgt
     1381 tattcgggcg cgcggaagct gtcgtggcag gatttgcagc ttgcgccggt ttcgccgtag
     1441 gcggctttga tttcgtccag tttgccggtt tgggcggcgg cgttgagttt ttcgacggcg
     1501 gcggcgaatt ttgttttttc ggcttcaaat tttgcaccat ccgaccaaac ggcgggcagt
     1561 gcgcgcccgt tgccttgcgg gtcggactca aaaagcgtaa acggtttttt gctgctgtcg
     1621 gcaaacgccg ctgccgcctg tttgaatttt tcgacatcgt aaggttcttc gcctttgacc
     1681 attttgccca tacgcgagaa gtcgggcatc atagatttga acgcggcggt gcggttttcg
     1741 gaaatttcgc ctttgggttg ggaaggtatt ccgctgcctc cgcaggcgga aaggagcagt
     1801 gtgatggcgg cagcagcaag gctgatttgg gttttcatat tgaatgtgtc ctgtcgtggt
     1861 ggtatggttg ttgtcatttt cagccggcgc gatgccgtct gaagggtatt atgatacctg
     1921 aaacaggttt gggaaacgag aaagaaagga aaaacaatgg ctgttttaat taccggtgct
     1981 tcggcaggat tcggcgaagc gatgtgccgc gcgtttgtcg gggcgggata ccgcgttatc
     2041 ggtgcggcgc gtcgtgcgga caggcttcag gccttggcgg atgaattggg tgctttgttt
     2101 taccctttgg aaatggacgt gtcgcgccgc gagtcggtgg aaaacgcctt aaacggcatc
     2161 cctgatgaat tttccgacat cgactgcctc atcaacaatg ccgggctggc tttgggtctg
     2221 gacacggcgg acaaggcgga ttttgaagat tgggaaacaa tgattcaaac caatgttttg
     2281 ggtttgacgt ttctaacgcg caagattttg ccgaatatgg tggctcgggg cggcggttat
     2341 gtgatgaatt tgggttcgat tgcaggcaat tatgcttatg ccggcagcaa cgtttacggg
     2401 gcgaccaagg cgtttgtgcg ccagttcagc ctgaatttgc gcgcggagtt ggcggataag
     2461 aacatccgcg ttaccaatat cgagccgggt ttgtgcggca atacggagtt ttccaacgtg
     2521 cgcttcaaag gcgatgacga gaaggcagca ggcgtgtatg agggtgtgga atttatccgc
     2581 cccgaagata ttgcggaaac cgcattgtgg ctgtaccgcc ggccggcgca tatgaatgtg
     2641 aacacgattg aaattatgcc cgtggcgcag acttttgcag gaatgaaggt gataaaaaaa
     2701 gccgtgccgg aagtgcggga agactttgaa aaacagagta tgtcgctgtt ttcccgcatc
     2761 agatcttggt tcaaatgatg cggaatgccg tctgaagaca gtttcagacg gcatttttac
     2821 gggcattttt acggagtagg caataagccc gccaatatgg ggttgccttc tttcggaatc
     2881 gggcgcggat tgccttccgc atcgatggcg acataagtga acacggcttc ggttacgagg
     2941 tggcggtctt cggtaacgca atcgttcatc aaagttttca cccagacttc gactttaagc
     3001 tggagggaag tattgcccac gcggacgcaa tgcccgtagc agcagacgac gttgccgacc
     3061 ttgaccgggc ggatgaagtt catttcctga acggcgacgg taacgatgcg tccttgtgcg
     3121 atttccgctg ccaatatgcc gccgcccaaa tccatctgcg acataatcca gccgccgaaa
     3181 atgtcttggt tgggattggt atcgcgcggc atagcgacgg tacgcaggag cagttcgcct
     3241 tgagggcgtt ggcggttgcc ttcttcgtgc tgcataaagt ttccttgttt tattgaaaga
     3301 taaatcgaac ctgcacccct gcccgaaacg ggaagtcggg aagtgcgtag tttaacccat
     3361 ttgagacata caagggcggg cgtgggacag attcgcaagg cgtattgtag ggcggggctg
     3421 tagagtgggc ttcagtccgc caatcccgcc aaatcctacc ctaagcaact gaaccgtcat
     3481 tcccacgaaa gtgggaatct agaatctcgg gggttcagtc atttccgata gattcccgcc
     3541 gcgtca
//
LOCUS       DAXHPG010000178         3488 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXHPG010000178 DAXHPG010000000
VERSION     DAXHPG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.178"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(1022..1792)
                     /gene="xth"
                     /locus_tag="ACFM6F_001913"
     CDS             complement(1022..1792)
                     /gene="xth"
                     /locus_tag="ACFM6F_001913"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6165274.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1852..2133)
                     /locus_tag="ACFM6F_001914"
                     /pseudo
     CDS             complement(1852..2133)
                     /locus_tag="ACFM6F_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
     gene            complement(2272..2511)
                     /locus_tag="ACFM6F_001915"
     CDS             complement(2272..2511)
                     /locus_tag="ACFM6F_001915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165275.1"
                     /translation="MSDKEPPNRNPVIASRRKCRLKYSSDGIFREGRDALEKTAFQAG
                     ILFARQRGGIGRAGERTGAAVLCCLKARLYACLQK"
     gene            complement(2539..3420)
                     /gene="nadC"
                     /locus_tag="ACFM6F_001916"
     CDS             complement(2539..3420)
                     /gene="nadC"
                     /locus_tag="ACFM6F_001916"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC6165276.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPEKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
BASE COUNT          854 a          837 c          873 g          924 t
ORIGIN      
        1 tgaaactgat gcagatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
       61 cagtacaaat agtacggcaa cgctgtactg gtttaaattt aatccactat attttgtaat
      121 agctgccatt atgcctacta ggatatatac cttctatata atccttattt aaataaaaac
      181 ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa gattttatct ttctcatcag
      241 gtttatattt gctatcaata tcaaaaagag aataaggatt acaatgagtt cgacatacta
      301 ggtaaaaatt tatatcttgg atcatgaaat attcgatatt tatcaggatc gaaagatgct
      361 aaaaattcat caatatcggt actggatatt ttgtcatcac taataaacat cgctagagat
      421 gggcgacttt gattttccat ttgttgatta gtaaaggata taaatactaa cttgccattg
      481 tcaaaccaca tagatttata tagtggatta aatttaaacc agtacgtcgt tgcctcgcct
      541 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
      601 tatagttatt gcctttggga gaatcaattc tgacagcaca taacataggt tcatctttac
      661 agtcgttata ttgggtttca tcaaactcaa ctgcatagca caataaaggc aataggaaaa
      721 aaaggagatt aatttattac tatacataag gcagcctgac tattcattct aaaataaatc
      781 cggtttttaa tatttgattc tgctccggat tatatacata ataaatttta ctgctccaaa
      841 atctgatatt aaccctatgg atttcataaa tttttcagaa aaataggctg tgcttgaaca
      901 atccgccgac tccggctgtt tgtcagggaa aaggcaatta ttttcggtta gtcagcagcc
      961 tatcctattg aatcacttaa agatatggat gtttgtcaac gccatatcaa aacacggtct
     1021 tttaccaatc gaattctgcc gtcaccggcg cgtggtcgct cggacgctcc agcgcgcgcg
     1081 tctccaaatc aacgcggaca tccttcaacg ccgccgccat cgcaggcgac accaaaatat
     1141 ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc gcgatagtcg aaccaggtat
     1201 agaacgcgcc ttcgggatgg acttggcgca ggctgtcggt cagtcccaaa tccagcaggt
     1261 ttttgaacca ctgccgttcg acggacgaac agtggatttt ttcgtgccat ttttcagggt
     1321 cgtaacagtc cgcatcggca ggcgcgatat tgaaatcgcc cagtagcacc agtttgccgt
     1381 agcgggtcat ttcatcgcgg acaaattcgg ttaacgcggc aaaccattgt tctttatatt
     1441 tgaatttcgg gctgtcgagg gcttcgccgt tgacgcaata gacattgatg acgcgcacac
     1501 cgccgacggt tgccgcaatc acgcgccgtt gcggatcgtc cggcagcgcg ggcagcccga
     1561 tatgcacgtc ttccggcgcg ttgcggctga cgattgccac gccgttgtag gttttctgcc
     1621 cgctccaaac acagtgccag cccatcattt gcaaagcggc ggccggaaat ttgtcctgat
     1681 cgagtttgag ttcctgcaaa accaaaatat cgggcggatt gtcggcaagc aggttttgca
     1741 cctgcggcag ccgcacattg agcgaattga cgttccaagt ggtgattttc ataacatttc
     1801 cgaaaaatgc cgtctgaaac gttcagacgg caaaaggaaa ggattttaac actataccgc
     1861 gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt gccgcgtctt cggaaatcag
     1921 gcggtattcg ataatgcggt gctaacgcgt aaagtccacc agaccttcca cgcgcagtcg
     1981 gctcaaatgg ttggaaaccg ctgtcgcagg catggacagg gattcggaca gctcggcaat
     2041 attgcaatcg ccgtccaaca gttggagcag tatcgccata cgttcgggat gcgccataag
     2101 tttgagtatg gtgtggagcg gtattgtgtt catcggtatt cctaaaacct tttccgcccg
     2161 ccaagcggat tgacgggcgg cagatatgga tgattgtgat tatctttata aattatatta
     2221 taaatcaatt atttatatat gattgtacag gattgtatgt tccttttacc actatttttg
     2281 caaacacgcg taaaggcggg ctttcagaca gcataaaact gccgcgcccg tcctttcccc
     2341 tgccctgccg atgcctccgc gctgtcgggc aaacaaaata cctgcctgaa acgctgtttt
     2401 ttctaaagca tcacgacctt cacgaaaaat gccgtctgaa gaatatttca gacggcattt
     2461 cctccttgag gcaataaccg gattacggtt aggcggttcc ttgtctgaca tatcagacaa
     2521 ctgccgacac cctaaaactc atgccaagaa atccaaacct atgtccaacg aacggctgct
     2581 gtgggtcaga tagccgagag cgatgccgtc cacgccggtt tgcgccacgc gcttcagtcg
     2641 gtcgaagccg atgccgcccg atgcttcgca atagacggtg tgggggtggg cggtttgcgt
     2701 gtggcagcgg tttgccgctt ctttcagggt ttcgtcgtcc atgttgtcca gcaaaatccg
     2761 ttccgcgccc gctgcgatgg cttcgtccag ttgtgccaac gtgtccactt cgatttctac
     2821 acaagtcaac ggtccgaccg cctgttttgc ctgccgaacg gcttgggcga tgctgccgca
     2881 ataggcgagg tggttgtctt tgatgagcac ggcgtcgtcc aagcccatgc ggtggttcac
     2941 gccgccgcct gccctgacgg cgtatttttg caggacgcgc agcaggggga tggttttgcg
     3001 gctgcacacg atgtctgtac cgtattcggc gacttcggca acggcacgcg cggtggcggt
     3061 ggcgatgccg cttaagtgcg tgaggtagtt gagcgcggtg cgttcggcgg cgagcagcgc
     3121 gcgggcgttg ccttcgacgg cggcaagcgt ctgacctgcg cggacgactt gcccgtctcg
     3181 gatttcggct tggaagcgaa cggacggatc catcgtctga aaggcgaggc gcgccaagtc
     3241 catgccggcg ataacgccgt cttcgcggct gacgaggaag agtttggcgg ttttttcggg
     3301 ggcgataacg gcggcggacg taatatcgcc gcgcctgccc aaatcttcgc tcaaggcttg
     3361 ttccaccatg gggcgcaaca gggtgtcggg cagggggaag agggtgtttt cagacggcat
     3421 ggtgtttcct ttgttgaaaa agattgatat tgcccgatgg ggcaaaactt cattttatag
     3481 tggattaa
//
LOCUS       DAXHPG010000179         3478 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXHPG010000179 DAXHPG010000000
VERSION     DAXHPG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.179"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            171..680
                     /locus_tag="ACFM6F_001917"
     CDS             171..680
                     /locus_tag="ACFM6F_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165277.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRSRNLPATTTAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAVSSQRAE"
     gene            complement(733..1080)
                     /locus_tag="ACFM6F_001918"
     CDS             complement(733..1080)
                     /locus_tag="ACFM6F_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6165278.1"
                     /translation="MYIPDKGDIFHLNFDPSGGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSGGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(1082..1318)
                     /locus_tag="ACFM6F_001919"
     CDS             complement(1082..1318)
                     /locus_tag="ACFM6F_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFC6165279.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDVGNEVV"
     gene            complement(1677..2168)
                     /locus_tag="ACFM6F_001920"
     CDS             complement(1677..2168)
                     /locus_tag="ACFM6F_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFC6165280.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACEIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            complement(2420..2590)
                     /locus_tag="ACFM6F_001921"
     CDS             complement(2420..2590)
                     /locus_tag="ACFM6F_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165281.1"
                     /translation="MPSEIQTAFLLKGFSSTALHKGGFFMPSESPSDGIGLHGRSSPA
                     FKQERMSRMPDF"
     gene            complement(2606..2974)
                     /locus_tag="ACFM6F_001922"
     CDS             complement(2606..2974)
                     /locus_tag="ACFM6F_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFC6165282.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMARLDGALSRIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
     gene            complement(2971..3102)
                     /locus_tag="ACFM6F_001923"
     CDS             complement(2971..3102)
                     /locus_tag="ACFM6F_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165283.1"
                     /translation="MQNQNTRPVKIELKGEAGKRVLLAAARRIAKTHQKAVKALADK"
BASE COUNT          889 a          843 c          799 g          947 t
ORIGIN      
        1 gcgctgaaat tcgaccagtt gatactcgaa tttcccgagc gcggggacgg cgcatgggta
       61 cacatcggtt tccgacgcaa cagcccgcaa cgcaaccaga tactgaccgc aaccaagaaa
      121 aacggcaaaa ccgtgtatct gcccgggctg catccttgag gtcatctgaa atggatattt
      181 tattgaaata ctggaagccg gtaggcgtat tgctgctgat agtcctgatt tttaccgcat
      241 ggcatttcga ccgtgccgaa aaataccgca tgggacggga ggctgctgct gccgaaattt
      301 cgaatcgtct gaaagacggc tatatcgagc aggcaaagca ggcgcgttct gccgagcaga
      361 aggccgctgc cgcgtttgcc gaacgacaaa ccaaattaga agaggaaaaa caaaatgctg
      421 aaaaaactgt tgccgctatg cgtcttgagc ttaaccgcct gcgccactac gccgccgccc
      481 aaaatcgcag cagaaacctg cccgcaacca ctaccgccgc caccgcatct gatggcgcgt
      541 cagattccca aggctggcta ttattcggac agtgcgctga aaaatatgca ggactggcag
      601 aaacagccga cggacatgca gccgatttaa gggaatggca ggcatatggt catgcagtaa
      661 gcagtcagcg tgccgaataa gcaaccgccc gaacctgtaa gaaaagatta caggttcggg
      721 cggtttcagc atttaatcga ataagacggc gccgatgcgc gccagcacat cgtccaatac
      781 ataatcgggt acagtttctt taaaagaagc cttgcggatt tgccagtcca gagatttgag
      841 ctggtggcag tggacattgc cctgcgtttc cgttcctgca ccgagtaagg ttgaaatcat
      901 gccgccgctt cgtgcagccg ctgcattccc ctgtgaaatg gggcaggcaa aaaccaatcc
      961 cgttgcgcgg ttgaatgctt ttggagacag agccagcgca aaccgcccgc ccttgatttc
     1021 cttgccgccg gaagggtcga aattcaaatg gaaaatatcg cctttgtcgg gaatatacat
     1081 ttcagacgac ctcgttgccg acatcatcca agatttccca gccttctacg cgcggcgggg
     1141 tttcttccat ttcggcaagc aagtctgcca agcggaaacg tcgggcagca cgcacacgga
     1201 gttcgccgtt atgtacttcc gctaccaaag cgtcgccgat tttaaaatcc aattgtttca
     1261 gcatgtcggc aggcagtcgg acggccgccg agttccccca tttttggaca cgcaacataa
     1321 tcttcacctt tattgtatct acaaagtaga tacatattac cataaaattt cagttgttca
     1381 aatacttgtg cagaatacgc caaaagccgt ccgaactgtt tcggacggct tttgtactgt
     1441 atttgcgcct tcaggcaata ttttgttatc cattttcaag atgcaaaagc tttctaattg
     1501 cttgatgtcg gatttcggtt gtttagggat acaaaaccaa gtaaactaaa actgtttgat
     1561 tggaaaatgc tccgcaagga gaaaattatg ttcaaaaaat cactttataa ggctgctttg
     1621 gcgtatttcg gcgattgcgt ggctgcccat atttcagaac agttttgact gtttattcac
     1681 aaatcagatg cctttagggg gtttgctttc cataatgcaa ccaaaatttc caactctcta
     1741 aatattgtgt ctttgcgttc ttcttcgcgt attttcataa caagaggcga aactgcattc
     1801 cacaatttta tgaagttggt gcaatgcagc cgtttaaaca aatcctcatc caatactccc
     1861 gagttgattt cgcacgcata aaactcgtgc cgattgatta cggtaagtaa atgccctctc
     1921 tctttttccc attgtccgcc cttattttga taaatttcat acaggtcgat ccgcgcgttg
     1981 ttgtcgtcaa ctgccgtgcc gcgaatataa ggcgaaatat gattgtcggc ttctacaaat
     2041 tgtgcatctt ggtaatcatt caaaataaca tctaatgtcg ccttttgctt tgaagttttc
     2101 ttattgatga atattgtccc cagtgcaatg acggcggtta ctgaaacaac ggtcagttgc
     2161 caaaacatca gccattcggc cgtccctcat aggttaaact gtacgctgtg cctgaatatg
     2221 taaaataaag tgaatacaga tacaaacaag gaggttaggg caattactga caaccaacct
     2281 ccttgtttta tttgtttccg caccttacca gccattaaaa ccctcatttt tgtatttcat
     2341 cgtatttcct taatggttgt aaccttgctt gtcgatatct gtatattaat gccgtgcgcc
     2401 cgttctttca aggggtgatt taaaaatcag gcatccttga catcctctcc tgcttaaagg
     2461 cgggggaact cctgccgtgt aaaccaatgc cgtctgaagg gctttcagac ggcataaaaa
     2521 aaccgccttt gtgtaaagcg gttgaagaaa agcctttcaa taaaaatgcc gtctgaattt
     2581 cagacggcat tgttgtcgga tatgcctatt ccttatccag tcggcgcagg gtttcggcga
     2641 gctgtttgaa gtcggtttcc cccgccgcca aactgacaat caagtcgtca agcccttggt
     2701 cggcggcaat gctgatgccc tgcaaatcca gataggtcaa cattgttaaa agcgcggtgc
     2761 gcttgttgcc gtcgggaaag gcgtgggctt tggctatggc ttgtgcataa agggcggcga
     2821 tttcgtagat gtcctcaagg ttttcatact gccgccagtt ggcaatccgc gacaatgcgc
     2881 cgtcgagccg cgccatatcc gcccgccctt tcaaacccgc ttcatccgcc aatacggttt
     2941 gatggataag cgcgaccagt tcgccgtcta tcatttgtcg gcaagtgcct tgacggcttt
     3001 ttgatgggtt ttggcaatgc ggcgggcggc ggcaagcagt acgcgtttgc ctgcttcgcc
     3061 tttaagctcg attttgacgg ggcgtgtatt ttggttttgc atcgctgctc cttgtctgtt
     3121 tgcgggcatt ttagcttttt tccggcagct tgggaaatgc cgtctgaaaa cacttcagac
     3181 ggcattcttc taatagtgta atgcaatagt tactccaaga tttttgtata tggctgtttt
     3241 tatggtaaat taaattttca caaagaataa atatatttaa atcaataaaa taaaatttag
     3301 tcgaatccca ccgtttccgc cagaaaacaa aaccgccctg attcagggcg gttctttttg
     3361 gcgcgaatct gcacaattac cataaaagaa tcattggatt atgcttattt tccgtaacgc
     3421 cctgcaataa accgcaatcg gcagatgctt tgatcacagt tttatggtaa tttatatg
//
LOCUS       DAXHPG010000180         3468 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXHPG010000180 DAXHPG010000000
VERSION     DAXHPG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.180"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            43..711
                     /locus_tag="ACFM6F_001924"
     CDS             43..711
                     /locus_tag="ACFM6F_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC6165284.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTVNNKCDSGKKGQGMLAFGDKNGNK
                     GYDNDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTTDRVVWTFNQNGTFGYTK
                     DQHLTKQSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRNDRRTV
                     CQYK"
     gene            741..1355
                     /gene="pilV"
                     /locus_tag="ACFM6F_001925"
     CDS             741..1355
                     /gene="pilV"
                     /locus_tag="ACFM6F_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC6165285.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHHTLSAVDGDFAVDA
                     VKSKKDLAKAQLDRFGYELKNALQDAAAIYYAVCKDSSGAAPTLSAGSTFSSNCDNKA
                     NGDTLIKVLWVNDSAGDSDIARTNLETNGNNIVYTYQARVGGRE"
     gene            1352..2320
                     /locus_tag="ACFM6F_001926"
     CDS             1352..2320
                     /locus_tag="ACFM6F_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC6165286.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHTEKDVVSDVPQKNRL
                     FSLKGSSANKLIPNGTDNKLIPITESLDINYQNFFQVGSALIFQYGIDDVNASAATTV
                     VSSCAAISKPGKQILTLENVKKELKISDQDKEQNGNIARQRHVVNAYAVGRIAGEEGL
                     FRFQLDDKGKWGNPQLLAKKIRYMKVRYIYVSDCPEDDDAGKEETFRYTDKFNSAQDA
                     VTPAGVEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2299..2892
                     /locus_tag="ACFM6F_001927"
     CDS             2299..2892
                     /locus_tag="ACFM6F_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC6165287.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRFCPANSTDLCIDKKGMEYKKGKGSVSKPPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2897..3370
                     /locus_tag="ACFM6F_001928"
     CDS             2897..3370
                     /locus_tag="ACFM6F_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC6165288.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIERGYQSQLYTE
                     MVGINNISKQFILKSPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLETLSSDVGCEAFSNRKK"
BASE COUNT          994 a          691 c          885 g          898 t
ORIGIN      
        1 aatttaatcc actatacatt accagttaac gttctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcacgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacggtca acaataaatg tgactccggc
      301 aagaaggggc agggaatgtt ggctttcggc gacaaaaacg gcaataaggg atatgacaat
      361 gatacggcgg atgttctcct ccgcagtgtg gtattgaatg atgatatcaa tgataagcgg
      421 attaattatg ccttcaacca tatcgctttt ggtcagactc agccgaccac cgaccgtgta
      481 gtttggacat tcaatcaaaa cgggacgttc ggttatacga aagaccagca tcttacaaaa
      541 caatccagct ttttttattc cgacggttat atccaaatcg tgctgacaga tgcgagagca
      601 gtttcagatg ccgataagaa attccgttcg gcggtggttt tgattgacag cagcggcagg
      661 gttgaggttt gtcctagaaa cgataggcgt accgtatgcc aatataaatg attgttttag
      721 tcgatttggg aaataaaaaa atgaagaata atgattgctt ccgcctgaaa aacccccagt
      781 ccggtatggc gctgatagaa gtcttggtcg ctatgctcgt tctgaccatc ggtattttgg
      841 cactattgtc tgttcagttg cggacagtcg cttccgtcag ggaggcagag acgcaaacca
      901 tcgtcagtca aatcacgcaa aacctgatgg agggaatgtt gatgaatccg accattgatt
      961 cggacagcaa caagaaaaac tataatcttt acatgggaaa ccatcataca ctatcagctg
     1021 tggatggcga ttttgcggtt gatgccgtga aaagcaagaa ggatttggca aaagcccagt
     1081 tggacaggtt cggttatgaa ttgaaaaatg ccttgcagga tgcggcagcc atctattacg
     1141 ccgtctgcaa ggattcgtcg ggtgctgcgc cgacattgtc cgccggcagt actttttctt
     1201 caaattgcga caataaggca aacggggata ctttgattaa agtattgtgg gtaaatgatt
     1261 cggcagggga ttcggatatc gcccgtacga atcttgagac gaacggcaac aatatcgtat
     1321 atacctatca ggcaagggtc ggaggtcggg aatgagacgt aaaatgctaa acgtaccaaa
     1381 aggcagttat gatggtatga agggttttac cattattgaa tttttggttg cgggcatgct
     1441 cagtatgatt gtcctgatgg cggtcggatc gagttacttc acgtcccgga aattaaatga
     1501 tgcggcaaac gagcgtcttg ctattcaaca ggatttgcgg aatgcggcaa cattaattgt
     1561 ccgcgatgca agaatggcgg ggagcttcgg ttgtttcaat atgtctgagc ataccgaaaa
     1621 ggatgttgtt tccgatgtgc cgcaaaaaaa ccgtcttttt tccttaaaag ggagcagtgc
     1681 aaataaactt atccccaacg gtacagataa taaacttatc cccataacgg aatctttaga
     1741 tatcaattat cagaattttt tccaggttgg tagcgcattg atttttcaat acggaatcga
     1801 tgatgttaat gcaagcgccg cgactaccgt cgtcagcagc tgtgccgcaa tatcgaaacc
     1861 gggcaagcaa atccttactt tagaaaatgt aaaaaaagaa ttgaagattt cggatcagga
     1921 taaggagcaa aatggcaata tagcgcgtca aaggcatgtg gtcaatgcct atgcggtcgg
     1981 caggattgcc ggtgaggaag gtttgttccg cttccaattg gatgataagg gcaagtgggg
     2041 taatcctcag ttgctcgcga aaaagattag atatatgaaa gtgcggtata tctatgtttc
     2101 cgactgtcct gaagatgacg atgccggcaa agaggaaaca ttcagatata cggataaatt
     2161 caacagcgcc caagatgctg ttacgcccgc cggggtggag gttttattga gtagcggtac
     2221 tgataccaag attgccgctt cttcagacaa tcatatttat gcttaccgta tcgatgcgac
     2281 aatacgcggg ggaaatgtat gcgcaaacag aacactttga cgggaatccc gacttctgac
     2341 ggacagaggg ggtttgcact gtttatcgtg ctgatggtga tgatcgtcgt ggcttttttg
     2401 gttgtaactg ccgcgcagtc ttacaatacc gagcagcgga tcagtgccaa cgaatcagac
     2461 aggaaattgg ctttgtcttt ggccgaggcg gctttgcggg aaggcgaact tcaggttttg
     2521 gatttggaat atgatacgga cagtaaggtt acatttagcg aaaactgtgg aaaaggtctg
     2581 tgtaccgcag tgaatgtgcg gacaaataat gataatgaag aggcttttga caatatcgtg
     2641 gtgcaaggca agcccaccgt tgaggcggtg aagcgttttt gccctgcaaa ttctaccgac
     2701 ctgtgcattg acaagaaagg gatggaatat aagaaaggca agggaagcgt cagcaaacca
     2761 ccgcgctata ttatcgaata tttgggcgtg aagaacggag aaaatgttta tcgggttact
     2821 gccaaggctt ggggtaagaa tgccaatacc gtggtcgtcc ttcaatctta tgtaagcaat
     2881 aatgatgagt aataaaatgg aacaaaaagg gtttacattg attgagatga tgatagtcgt
     2941 cgcgatactc ggcatcatca gcgtcattgc cataccttct tatcagagtt atattgaaag
     3001 aggctatcag tcccagcttt atacggagat ggtcggtatc aacaatattt ccaaacagtt
     3061 tattttgaaa agtcccctgg acgataatca gaccatcaag agcaaactgg aaatatttgt
     3121 ctcaggctat aagatgaatc cgaaaattgc cgaaaaatat aatgtttcgg tgcattttgt
     3181 caataaggaa aaaccaaggg catacagctt ggtcggcgtt ccaaagacgg ggacgggtta
     3241 tactttgtcg gtatggatga acagcgtggg cgacggatac aaatgccgtg atgccgcttc
     3301 tgcccgagcc catttggaga ccttgtcctc agatgtcggc tgtgaagcct tctctaatcg
     3361 taaaaaatag ggctgttttg ccgatgccgt ctgaaaatca atgttcagac ggcattttta
     3421 tgggtatagt ggattaacaa aaatcaggac aaggcgacga agccgcag
//
LOCUS       DAXHPG010000181         3453 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXHPG010000181 DAXHPG010000000
VERSION     DAXHPG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.181"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(214..1278)
                     /locus_tag="ACFM6F_001929"
     CDS             complement(214..1278)
                     /locus_tag="ACFM6F_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6165289.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEDIASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVANRFTINHIADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="ACFM6F_001930"
     CDS             complement(1279..2037)
                     /locus_tag="ACFM6F_001930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6165290.1"
                     /translation="MKKVSVLIVARNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFQRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="ACFM6F_001931"
     tRNA            complement(2112..2187)
                     /locus_tag="ACFM6F_001931"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..3377)
                     /locus_tag="ACFM6F_001932"
                     /pseudo
     CDS             complement(2241..3377)
                     /locus_tag="ACFM6F_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            complement(3322..>3453)
                     /locus_tag="ACFM6F_001933"
     CDS             complement(3322..>3453)
                     /locus_tag="ACFM6F_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6165291.1"
                     /translation="FADRQNHINGIENFWNQAKRVLRKYSGLARTFPRIGSATGCAD"
BASE COUNT          780 a          845 c          913 g          915 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgat gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta tttttaagga
      121 tggattcggg gtgtttcttt ccaatgaacg aatataattt ggcggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc ggcttatatt gcatccaata atttgtcggc
      241 gatatggttg atggtaaaac ggttggcgac gcattgatgc cctgccgttg ccagtgcgtt
      301 ttttgcttcg gggtttaaga cgaggcgttc gatttcatcc gccaaagatt caggtgtcag
      361 cctgtccagc agaatccccg attgatgatg ttcgataatt tccttttgcg cgcccaaagt
      421 attggaaatc actgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggcgg taaaacgaag caatgtcttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatccggat gtccgtgccc tgccaatttg agtctgattt gaggatattt
      661 ccgatgcagt atcacacagg cttcaatcag gttttccaat cctttttccg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaacg
      781 gccggtatcg ataccgttat gaacaatccg gtatttttct ttaaagggat tgtcggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tctgtgtttt tctgtatcag
      901 gcggtgataa aaatcggttt tgttggcgac gacattgtgt ttgacaaata tcaggcgcac
      961 cccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggtttttcc gaataaagcg ggtaagcgcg cagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgtgtcg tgaaaacgga agaaacttcg gaaaagcgtc tgtgcatcaa
     1141 ctcattattt ttattgacgg cggtaaacat cgtgcagccc cgcagtccca atgcttttga
     1201 aacatcatag acatattgtt cgcccccgcc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccggatttg tacagataat atagtttgac
     1321 atatttaatc attgtgtaat agctgtggtt gacggacata atccaaccca tttttccatc
     1381 gagaaaccct ttgttcagga tataaatttt gaaaaacccc caaattgggc ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg aaatggaagt
     1501 atatttgttg aatttgttga aatattgttc ccagttgtcg tacgtataat ggtacataaa
     1561 atgtttcaga cggcgtttag ggtagggggt ctgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgcct ttcttcggca tcaaacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ttcgacaaaa taggcggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattctccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccgttcaaa tgccgttgga agacttttgc gcccaaaccc tcggcaattt cggcagtatt
     1921 gtcggtgctg tggtcatcga taacgataac ttctttatcg aaacggcaac tttcaataca
     1981 ttcccgaatg tggtttgctt catttctagc aacaatcaat acgctgactt ttttcattat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtgggattga caaacttatt tcatccgatg attaagacgc agttgcttcc cccgaaggta
     2281 aacgatgagc tggcggcaat gcgtttttcg gtttcccagc ggctgccgct gccggtcagg
     2341 ttgatggcgg gaaggttcgg atcgttctgc ccgtcccaaa gctggggcgg aagttttcct
     2401 tcgggattgc tttgccggtc ggcaatgccc cacgcgaacg cggcttcgat tgcgcccgcc
     2461 gcacccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcacggct ttccatactg tcgttgtggt gcgtcccggt gccgtgcaga
     2581 ttaatccagc cgatgtcttc gggcgcaaga cctgcgtgtt gcaatgccgt ctgaaaggca
     2641 aggattgcgc cttgcgcgtc ggggcggggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattatc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacactt cggacgagaa aaattcttcg cctccgcccg ccagcatcat atcggtcaga
     2941 ccgtatttga tggcttcgta ggcataacct atgccttggc tgccggacga acacgcgctc
     3001 gatgtcggga tgatgcgtcc tttcagtccg aaaaagatgc cgatattggc ggcggcggtg
     3061 tgcggcatca tacgcacata ggtgttggca ctgaagttgc gcgacgtgcc ggtcagcaac
     3121 aattcgccca catcgccgat gtctttggtg ctgccgctgg aagagccgca ggcaacgccc
     3181 atccgtccgt cggtaatgct ttcgtctccg agcaaaccgg catccgtcaa agcctgctcc
     3241 gccgcatcga cgcacaaata cgacacacgc cccatacttc tgagctgctt gcgcgtccaa
     3301 tgtttcggcg gcgcgtaatc ctcaatcggc gcacccagtt gcgcttccaa ttcggggaaa
     3361 cgttcgtgcc aatccactat attttcgcaa gacgcgtttt gcctgattcc aaaagttctc
     3421 aatgccgtta atgtggttct gacggtctgc aaa
//
LOCUS       DAXHPG010000182         3420 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXHPG010000182 DAXHPG010000000
VERSION     DAXHPG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.182"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..330
                     /locus_tag="ACFM6F_001934"
     CDS             <1..330
                     /locus_tag="ACFM6F_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6165292.1"
                     /translation="RDGEDGGLLCKFFPDILACDRLPEPNPAEDLNLPSETVNVEFQK
                     SGIFQNSAQCPAPVTFTVTVLDSSRQFAFSFENACTIAERLRYMLLALAWAVAAFFCI
                     RTVSREV"
     gene            634..918
                     /locus_tag="ACFM6F_001935"
     CDS             634..918
                     /locus_tag="ACFM6F_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC6165293.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     SSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            919..2106
                     /locus_tag="ACFM6F_001936"
                     /pseudo
     CDS             919..2106
                     /locus_tag="ACFM6F_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin family protein"
     gene            2321..3277
                     /locus_tag="ACFM6F_001937"
     CDS             2321..3277
                     /locus_tag="ACFM6F_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HFC6165294.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQHQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDYNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT         1123 a          736 c          774 g          787 t
ORIGIN      
        1 agggacggcg aagacggcgg ccttttgtgc aaattcttcc ccgacattct cgcttgcgac
       61 aggctgcccg agcccaatcc ggcagaagat ttaaatctgc cgtctgaaac cgtcaatgta
      121 gagtttcaga aatcaggaat ctttcaaaat tccgcacagt gtcccgcacc tgtcactttc
      181 acagtgactg tgcttgattc cagcaggcag ttcgcgttca gctttgagaa cgcatgtacc
      241 atagccgaac ggctaaggta catgcttctc gcccttgctt gggcggttgc cgcatttttc
      301 tgtatccgca cagtatcccg tgaagtctag caggcgcagc accgccgggc ttcagtaact
      361 tgtaccaagg caggaggagg acgtccagaa agatttgtaa agacggcttt atcgtcttta
      421 taaatctttt tggatacccc ttgccgcccc gccaaaagaa tacactctgc cgcaagggca
      481 ggtggtaagg cgcgcgcttt ttgcgccgtt ccccctgccc ccgcggcgtc gcaagtgaga
      541 ctgggggtgt gggggctagt ccccgcaaaa tctttcagat taagaaacat ttttttaatg
      601 aggcaaccgt gccttttaag aaagggatag caaatgaaat tgttggccgc attgattccg
      661 ctgttgatga gcgtcgcagg ccgtatattg actgcattag gcttgatggc tgttacttat
      721 tcgggtgtag atagattggt agcccatttt cagcaggcga taaccagtag cataacgggc
      781 gcgcctcaag cgatgttgca gctcttttat ataagcggtg gcggcaccgt tcttaatatc
      841 ctgtttggcg cgatcgcctt tattctgtca ttcaaacaaa tgacaaaact agcaacctca
      901 atcgggaaga aaaaataaat ggcagagatc tgtttgataa ccggcacgcc cggttcaggg
      961 aaaacattaa aaatggtttc catgatggcg aatgatgaaa tgtttaagcc tgatgaaaac
     1021 ggcatacgcc gtaaagtatt tacgaacata aaaggcttga aaataccgca cacctacata
     1081 gaaacggacg caaaaaagct gccgaaatcg acagatgagc agctatcggc gcatgatatg
     1141 tacgaatgga taaagaagcc cgaaaatatc gggtctattg tcattgtaga tgaagctcaa
     1201 gacgtatggc cggcacgctc ggcaggttca aaaatccctg aaaatgtcca atggctgaat
     1261 acgcacagac atcagggcat tgatatattt gttttgactc aaggtcctaa gcttctagat
     1321 caaaatctta gaacgcttgt acggaaacat taccacatcg cttcaaacaa gatgggtatg
     1381 cgtacgcttt tagaatggaa aatatgcgcg gacgatcccg taaaaatggc atcaagcgca
     1441 ttctccagta tctatacact ggataaaaaa gtttatgact tgtacgaatc agcggaagtt
     1501 cataccgtaa ataaggtcaa gcggtcaaag tggttttaca ctctgccagt aatagtattg
     1561 ctgattcccg tgtttgtcgg cctgtcctat aaaatgttga gcagttacgg aaaaaaacag
     1621 gaagaacccg cagcacaaga atcggcggca acagaacagc aggcagtact tccggataaa
     1681 acagaaggcg agccggtaaa taacggcaac cttaccgcag atatgtttgt tccgacattg
     1741 tccgaaaaac ccgaaagcaa gccgatttat aacggtgtaa ggcaggtaag aacctttgaa
     1801 tatatagcag gctgtataga aggcggaaga accggatgcg cctgctattc gcatcaaggg
     1861 acggcattga aagaagtgac ggagttgatg tgcaaggact atgtaaaaaa cggcttgccg
     1921 tttaacccat acaaagaaga aagccaaggg caggaagttc agcaaagcgc gcagcaacat
     1981 tcggacaggg cgcaagttgc cacattgggc ggaaaaccgt agcagaacct aatgtacgat
     2041 aattgggaag aacgcgggaa accgtttgaa ggaatcggcg ggggcgtggt cggatcggca
     2101 aactgaagaa aacggcaaga gagaaaaaag acccgtaaac cgtttgaata tagacggttt
     2161 acgggtcttt gtttcgcgca aagcaagggc taaggcagtc aggcagcaaa tcccgcaatg
     2221 tattaaaaca gacgcgtaga aatgccggct gcctttatcc atcctcaaaa ttgaatatca
     2281 tcctagccgt atcaaggctg tataaataag gaaaatacca atgaatataa tcgggctgga
     2341 catctcaaag gataccatag acgcaacatt gcataaaaca aacggaagta tccattacat
     2401 taaatttaag aataatgatg atggattaaa acagtttaga ttgtggataa agggaaacag
     2461 aatcagaaaa gcctatatcg gcatggaggc aacaggcatc tattacgaaa aggcagcaga
     2521 tatgctttct tcctactata ccgtttacgt tatcaatccc ttaaaaatca aggactacgg
     2581 gaaaagcagg tttaaccgta ccaaaaccga caaagcagat tcaaacctga tagcagatta
     2641 cataaaaagg catcaagata cattgatacc gtatcagata cccaaaaaca aagcactgca
     2701 aaaactgatt aaccttaaaa atcaattaca gcaacatcag aagcaaatta aaaaccgtct
     2761 tcatagcact gaagaagact tcataaggaa catacatcaa gacttgatag ataccataca
     2821 ggacaagatg gaacaggtaa aaatagccat atccgaacaa atcaaaaaac aaacggacta
     2881 taaccattac cgcaatcttc aaaccatccc gagcataggc aaagacaccg catcagttct
     2941 ttatgcgcaa ctgacagaaa aacattttaa aaccgcaaac cagtttgtat cctatgccgg
     3001 attaagtccc gccatcatac aatcagggac aagcgtaaga ggtcggggca gattgagccg
     3061 atacggaaac agacgattaa aaagtacgct gtatatgccc gccctttgtg cttaccgttt
     3121 taacgcattt ccgaaattaa taaataatct gaaaaaagcg ggtaagccaa agatggtaat
     3181 tatcgttgcc atcatgcgca aactggcgaa gctcgcctat tatattgtta aaaccggcca
     3241 gccttacgat gcggaaagac accgattgaa tcaataaaat tcaacaaaat taaacggtta
     3301 cgcgaatata tttgtgtaac cgtgcatttg catatcgtaa ataaacgtaa ataaaaataa
     3361 caatataaat cagtatattg caactttgtt ttttattttg tgttgacggg caacatatca
//
LOCUS       DAXHPG010000183         3402 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXHPG010000183 DAXHPG010000000
VERSION     DAXHPG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.183"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     regulatory      65..168
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            235..1458
                     /gene="cytX"
                     /locus_tag="ACFM6F_001938"
     CDS             235..1458
                     /gene="cytX"
                     /locus_tag="ACFM6F_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC6165295.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVLV
                     RLFFKKTQSLQRNPS"
     gene            1455..2555
                     /locus_tag="ACFM6F_001939"
     CDS             1455..2555
                     /locus_tag="ACFM6F_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC6165296.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVIKLGRQSIPLWRNIRGHLKTPAMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEGLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            2576..3193
                     /gene="thiE"
                     /locus_tag="ACFM6F_001940"
     CDS             2576..3193
                     /gene="thiE"
                     /locus_tag="ACFM6F_001940"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC6165297.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCVAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          682 a         1072 c          945 g          703 t
ORIGIN      
        1 aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg ccccctgctt ttgtgtacac
       61 ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag attgcgaaag cagaatccgt
      121 agaacctgtc ggggtaatgc ctgcgtagga aacaaaccgt caaatgcctt atcaggcttc
      181 cgttcccttt tccgcacttc cccgcccacc ttccttcttt ttaaaggacg tcatatgtcg
      241 ggcaatgcct cctctccttc atcttccgcc gccatcgggc tgatttggtt cggcgcggcg
      301 gtctcgattg ccgaaatcag cacgggtacg ctgcttgcgc ctttgggctg gcaacgcggt
      361 ctggcggctc tacttttggg tcatgccgtc ggcggcgcgc tgttttttgc ggcggcgtat
      421 atcggcgcac tgaccggacg cagctcgatg gaaagcgtgc gcctgtcgtt cggcaaacgc
      481 ggttcagtgc tgttttccgt ggcgaatatg ctgcaactgg ccggctggac ggcggtgatg
      541 atttacgccg gcgcaacggt cagctccgct ttgggcaaag tgttgtggga cggcgaatct
      601 tttgtctggt gggcattggc aaacggcgcg ctgattgtgc tgtggctggt tttcggcgca
      661 cgcaaaacag gcgggctgaa aaccgtttcg atgctgctga tgctgttggc ggttctgtgg
      721 ctgagtgccg aagtcttttc cacggcaggc agcaccgccg cacaggtttc agacggcatg
      781 agcttcggaa cggcagtcga attgtccgcc gtcatgccgc tttcctggct gccgcttgcc
      841 gccgactaca cgcgccacgc gcgccgcccg tttgcggcaa ccctgacggc aacgctcgcc
      901 tacacgctga ccggctgctg gatgtatgcc ttgggtttgg cagcggcgtt gttcaccgga
      961 gaaaccgacg tggcaaaaat cctgctgggc gcaggtttgg gtgcggcagg cattttggcg
     1021 gtcgtcctct ccaccgttac cacaacgttt ctcgatgcgt actccgccgg cgcgagtgca
     1081 aacaatattt ccgcgcgttt tgcggaaaca cccgtcgctg tcggcgttac cctgatcggc
     1141 acggtgcttg ccgtcatgct gcccgttacc gaatatgaaa acttcctgct gcttatcggc
     1201 tcggtatttg cgccgatggc ggcggttttg attgccgact ttttcgtctt gaaacggcgt
     1261 gaggagattg aaggctttga ctttgccgga ctggttctgt ggctggcggg cttcatcctc
     1321 taccgcttcc tgctctcgtc cggctgggaa agcagcatcg gtctgaccgc ccccgtaatg
     1381 tctgccgttg ccattgccac cgtactggtg cgccttttct ttaaaaaaac ccaatcttta
     1441 caaaggaacc cgtcatgacc cgtatcgcca tcctcggcgg cggcctctcg ggaaggctga
     1501 ccgcactgca gcttgcagaa caaggttatc agattgcact tttcgataaa ggctgccgcc
     1561 ggggcgaaca cgccgccgcc tatgttgccg ccgccatgct cgcgcctgcg gcggaagcgg
     1621 tcgaagccac gcctgaagtc atcaaactgg gcaggcagag catcccgctt tggcgcaaca
     1681 tcagaggcca tctgaaaacg cctgccatga tgcaggaaaa cggcagcctg attgtgtggc
     1741 acgggcagga caagccatta tccagcgagt tcgtccgcca tctcaaacgc ggcggcgtgg
     1801 cggatgacga aatcgtccgt tggcgcgcag atgaaatcgc cgaacgcgaa ccgcaactcg
     1861 gcggacgttt ttcagacggc atctacctgc cgaccgaagg ccagctcgac gggcggcaaa
     1921 tattgtctgc acttgccgac gctttggacg aactgaacgt cccctgccat tgggaacacg
     1981 aatgcgtccc cgaaggcctg caagcccaat acgactggct gatcgactgc cgcggctacg
     2041 gcgcaaaaac cgcgtggaac caatcccccg agcacaccag caccctgcgc ggcatacgcg
     2101 gcgaagtggc gcgggtttac acacccgaaa tcacgctcaa ccgccccgtg cgtctgctcc
     2161 atccgcgtta tccgctctac atcgccccga aagaaaacca cgtcttcgtc atcggcgcga
     2221 cccaaatcga aagcgaaagc caagcccccg ccagcgtgcg ttcagggttg gaactcttgt
     2281 ccgcactcta tgccgtccac cccgccttcg gcgaagccga catcctcgaa atcgccaccg
     2341 gcctgcgccc cacgctcaac caccacaacc ccgaaatccg ttacaaccgc gcccgacgcc
     2401 tgattgaaat caacggcctt ttccgccacg gtttcatgat ctcccccgcc gtaaccgccg
     2461 ccgccgtcag attggcagtg gcactgtttg acggaaaaga cgcgcccgaa cgtgatgaag
     2521 aaagcggttt ggcgtatatc cgaagacaag attaaagccg tctgaaagga accccatgac
     2581 cttcccgcca ctcaaatccc cgctcaaatt ctacgccgtc gtccccaccg ccgattgggt
     2641 ggggcgcatg gtcaaagcag gtgccgacac ggtgcaactg cgctgcaagg ccctgcacgg
     2701 cgatgaattg aaacgcgaaa tcgcccgctg tgtcgccgcc tgtcagggca gccatacgca
     2761 gcttttcatc aacgaccact ggcgcgaggc aatcgaagca ggcgcatacg gcgtgcatct
     2821 cgggcaggaa gatatggaca ccgccgacct cgccgccatt gccgccgccg gtttgcgctt
     2881 gggtttgagt acgcactccg tcgccgaact cgaccgcgcc ctgtccgtac accccagcta
     2941 tgtcgccagc ggcgcgattt tcccgaccac gaccaagcaa atgcccaccg ccccgcaagg
     3001 cttggacaaa ctgtgcgagt atgtgaaaca ggcaggcggc acgcccgtcg tcgccatcgg
     3061 cggtatcgac ttgaacaacg cccgagccgt actcgccacc ggcgtttcct cactcgccgc
     3121 cgtccgcgcc gtaaccgaag cggcaaatcc cgaagcggtg gttaaagcgt ttcaggcttt
     3181 gtgggatgga taaaaccgaa agaagaaaat tcaattgccg tgtcggcaaa acttagcccg
     3241 ttatcgcaaa catacttaac tttaaatgtg gcatatcatc aaattccgtc attcccgcgt
     3301 aagcgggaat ccgccttaaa acttgagaaa ccatcatttg aaaaacagtt tccgaatttc
     3361 aaaaatggat tcccgcccgt gcgggaatga cggcaaccgg tc
//
LOCUS       DAXHPG010000184         3350 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXHPG010000184 DAXHPG010000000
VERSION     DAXHPG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.184"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            123..2150
                     /gene="uvrB"
                     /locus_tag="ACFM6F_001941"
     CDS             123..2150
                     /gene="uvrB"
                     /locus_tag="ACFM6F_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC6165298.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIADLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDTIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKESLLFGAE"
     gene            2186..3070
                     /locus_tag="ACFM6F_001942"
     CDS             2186..3070
                     /locus_tag="ACFM6F_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165299.1"
                     /translation="MTSIILELQSLAANPNSDVEELVSKTLMVARKLKINELTEWCNN
                     ELQGYSSKNLPKYRIFYGELKAFNPYRGDVPFMLPDELRERVTRVEYTSSISEIRNLL
                     KTKNNNFIRILSSKEKQFLMNLQDTPFPLEPKIIIYPINLMNIQSKVRQLILAWALRL
                     EEEGILGDGIQFTKSKQEKAMSNQFNIQNMQGFIGDIFGGNVQQNIQDGIHIEKENFN
                     KLANFLAKNGIPSAELEDLRNALIEDGQPTDKKFGSKVSAWIGNIITKASTGAIDVSV
                     GTIAGILTNAISKYYGLM"
     gene            3082..3300
                     /locus_tag="ACFM6F_001943"
     CDS             3082..3300
                     /locus_tag="ACFM6F_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165300.1"
                     /translation="MCGSTTHAVPNRPKPRACLRLTPYHPTNTASVGWILVSDKTFNI
                     PIIAIHCSNAQNVGFENPTYKIIPSIIL"
BASE COUNT         1028 a          879 c          687 g          756 t
ORIGIN      
        1 tgggtagcga ttgggtttca gacggcatac attcaggatt tcaccgcctt atgccgtctg
       61 aaagacgttt ccgacccatc cctactacac gctttgtccg caaacgcatc acaggcaaga
      121 ttatggaagt cattcgatac cccgattccc ccttcaaact ccaccaaccc ttcccgcccg
      181 caggcgacca gcctaccgct attgccgact tgctcgaagg gctttcagac ggccttgcct
      241 accaaaccct gctcggcgta accggttcgg gcaaaaccta caccatggcg aacgtcatcg
      301 cccaaagcgg ccgacccgcc atcatcatgg cgcacaacaa aacccttgcc gcccagcttt
      361 atgccgaaat gcgcgagttt ttccctgaaa acgcggtgga atatttcgtc tcctactacg
      421 actattacca acccgaagcc tatgtgccaa gccgcgattt gttcatcgaa aaagacagtg
      481 cgatcaacga acacattgaa caaatgcgcc tttctgccac caaaaacctg atgacgcgcg
      541 atgacgtgat tatcgttgcc actgtgtccg ccatttacgg tatcggcgac ccgactgagt
      601 atcaacaaat ggtgttgtcc gtcaaagaag gcgacaccat cgagcagcgc gacatcatcg
      661 ccacgctcgt ttccatgcag tacgaacgcg gcgatttgga tttcaaacgc ggcagcttcc
      721 gcgtgcgcgg cgacgtgatt gacgtgtacc ccgccgaaag ctccgaaaac gccttgcgta
      781 tcagcctgtt tgacgacgaa atcgaccgcc tcgatatgtt tgacccgctt tcaggcagcc
      841 tgcaccaacg cgtcggccgc tacaccgtct tcccgtccag ccactacgtt accccgcgcg
      901 acaccgtatt gcgcgcctgc gagtccatca aagaagaatt gcgcgaacgc attgatttct
      961 ttaccaaaga aaaccgtccc gtcgagcaac aacgcatcga acagcgcacc cgtttcgacc
     1021 tcgaaatgct ctacgaaatg ggcttctgca aaggtatcga aaactactcc cgtcacttct
     1081 ccggcaaaaa agaaggcgaa ccgccgccca cgctgatgga ctacctgccc gacaacgcca
     1141 tcatgttcat cgacgaaagc cacgttaccg tcacacaaat cggcggcatg tacaaaggcg
     1201 acgcatcgcg caagcaaaac ctcgtcgatt acggcttccg cctaccttcc gcccgcgaca
     1261 accgcccgct caaattccac gaatttgaaa aagtcatgcc gcaaaccgtc ttcgtttccg
     1321 ccacccccgc caaatacgaa gaagaacacg ccggacaagt ggtcgaacaa gtcgtccgcc
     1381 ccacagggct ggtcgaccct caaatcatca tccgccccgt cgccacccaa gttgatgact
     1441 taatgagcga aatcaacgac cgcatccaaa aaggcgaacg cgtactcgtt accaccctta
     1501 ccaaacgcat ggcggagcaa ctcaccgact attacagcga actcggcatc aaagtgcgct
     1561 acctgcacag cgacatcgac accgtagagc gcgttgaaat tattagagat ttacggctcg
     1621 gattgtttga cgtactcgtc ggcatcaatc tcttgcgcga aggcctcgac atcccagaag
     1681 tctccctcgt cgccatcctc gacgcagaca aagaaggctt cctgcgctcc caccgcagcc
     1741 tgattcaaac cataggccgc gctgcgcgca acgtgaacgg cgtcgcgatt ctgtacgccg
     1801 acaaaatcac cgactctatg aaagccgcca tcgacgaaac cgaacgccgc cgtgaaaaac
     1861 aaattaaatt caacgaagaa cacggcatcg tgccgcaaca gattaaaaaa caggtcaaag
     1921 acatcattga cggcgtgtac cacgaagaag acagcggcaa aggccgtaga caaggcaaaa
     1981 acaaagttaa agtcggcgaa attcacaacg aagaagacac gattaaagaa atcgccaaac
     2041 tcgaaaaagc catgcaacag gcggctaggg atttgcagtt tgaagaagcg gctgttcttc
     2101 gtgataggat acggaatatt aaagagagtt tgttgtttgg ggcggaataa ttttattatt
     2161 tttatttaac atggagatta attaaatgac ttctattatt ttagaactcc aaagcctagc
     2221 tgccaatcca aatagtgatg tagaagagtt agtgagtaaa acattaatgg ttgcaagaaa
     2281 attaaaaatt aatgaattaa ctgaatggtg taataatgaa ttacaaggtt attcatcaaa
     2341 aaatttacca aaatatagaa tattttatgg agagttaaaa gcttttaacc cttatcgtgg
     2401 cgatgttcca tttatgcttc ccgatgaatt gagagaacga gtaacaagag ttgaatatac
     2461 ctcatcaatt agcgaaataa gaaacttact aaaaacaaaa aataataatt ttatacgaat
     2521 tctatctagt aaagaaaaac agtttttaat gaatttacaa gatactccat ttccacttga
     2581 acctaaaata attatttatc caattaactt aatgaatatt caatcaaaag taagacaact
     2641 aattttagct tgggcattac gactggaaga ggaaggtatt ttaggagacg gtatccaatt
     2701 tacaaaatca aaacaggaga aagcaatgtc aaaccaattc aatattcaga atatgcaagg
     2761 gtttattggg gatatttttg gcggtaatgt tcagcaaaat attcaagacg gtattcatat
     2821 tgaaaaagaa aatttcaata aactggctaa ctttttagca aaaaacggga ttccatctgc
     2881 cgaattagaa gatttacgaa atgcactgat agaagatggt cagcccacag ataaaaaatt
     2941 cggctcaaaa gtaagtgcat ggattggcaa tattattaca aaggcttcta ccggtgcaat
     3001 tgatgtttca gtaggtacta ttgcaggaat attaactaat gcgatttcaa aatattatgg
     3061 cttaatgtaa gcaaatgcaa tatgtgcggc tccaccacac acgccgtccc caaccgccca
     3121 aaaccgcgtg cctgcctgcg gcttaccccc taccacccaa ccaacacagc aagcgtaggt
     3181 tggatacttg tatccgacaa aacctttaac attcccatca ttgcaatcca ttgtagcaat
     3241 gcccaaaatg tcggattcga gaatccgacc tacaaaatca ttccgagcat aatactatga
     3301 aataccgtcg tttttaccgc aatggcggca cttacttttt tacggttgta
//
LOCUS       DAXHPG010000185         3344 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXHPG010000185 DAXHPG010000000
VERSION     DAXHPG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.185"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            61..690
                     /locus_tag="ACFM6F_001944"
     CDS             61..690
                     /locus_tag="ACFM6F_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC6165301.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGDKPYEYGKPLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            865..3243
                     /gene="hmbR"
                     /locus_tag="ACFM6F_001945"
     CDS             865..3243
                     /gene="hmbR"
                     /locus_tag="ACFM6F_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
                     /protein_id="HFC6165302.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVLAADEAATETTPVKAEIKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIEIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLDDRQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGNRGYAVEGEGSGANIRGSARGIPDSSKHKYHSFLGKIAYQINDNHRIVGASLN
                     GQQGHNYTVEESYNLTASSWREADDVNRRRNANLFYEWMPDSNWLSSLKADFDYQKTK
                     VAAVNNKGSFPMDYSTWTRNYNQKDLDEIYNRSMDTRFKRFTLRLDSHPLQLGGGRHR
                     LSFKTFVSRRDFENLNRDDYYFSGRVVRTTSSIQHPVKTTNYGFSLSDQIQWNDVFSS
                     RAGIRYDHTKMTPQELNAECHACDKTPPAANTYKGWSGFVGLAAQLNQAWHVGYDITS
                     GYRVPNASEVYFTYNHGSGNWLPNPNLKAERSTTHTLSLQGRSEKGMLDANLYQSNYR
                     NFLSEEQKLTTSGTPGCTEENAYYGICSDPYKEKLDWQMKNIDKARIRGIELTGRLNV
                     DKVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLT
                     YLGAKKAKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPAKNLTLRAG
                     VYNVFNRKYTTWDSLRGLYSYSTTNSVDRDGKGLDRYRAPSRNYAVSLEWKF"
BASE COUNT          909 a          860 c          833 g          742 t
ORIGIN      
        1 cgggcaagtg cggattttgc ccgcaccgca tccttatccc ttaccgacaa aggaaaaaat
       61 atgagtgaaa ccgaaaatca agcattgaca tttgccaaac gcttgaaggc ggataccacg
      121 gcggttcacg acagcgtgga taacctcgtt atgtctgtcc aaccgtttgt cagcaaagaa
      181 aactacatca aatttttgaa actccaatcc gttttccaca aggctgtcga ccacatctat
      241 aaagatgccg aattaaacaa agccattccc gagctggaat acatggcgcg atacgatgcc
      301 gtaacgcaag accttgcaga tttgggtgac aaaccttacg aatacggcaa accgctgccg
      361 catgaaaccg gcaacaaagc aatcggctgg ctttattgcg ccgaaggatc caatttgggc
      421 gcggcgtttt tgttcaaaca cgcccaaaaa ctcgattaca acggcgaaca cggcgcgcgc
      481 cacctcgctc cgcatcccga cgggcgcggc aaacactggc gtgccttcgt cgagcatctg
      541 aacgctttga acctgactcc cgaagccgaa gcggaagcca tccaaggcgc gcgcgaagcc
      601 tttgcattct acaaagtcgt gttgcgcgaa accttcggtt tggcagccga tgccgaagca
      661 ccggaaggaa tgatgccgca caggcactaa aaaataatcg aaccaaataa acaaggtctc
      721 ggcatagctg tttgcaggga cctttaatta cacggcgcgg ctttgtttac atggattact
      781 gtcttattaa atattaatga ttatcataaa atctattatt cgctaaccga tggatgaaca
      841 atccatacat cttgagttga taatatgaaa ccattacaaa tgctccctat cgccgcgctg
      901 gtcggcagta ttttcggcaa tccggtcttg gcagcagatg aagctgcaac tgaaaccaca
      961 cccgttaagg cagagataaa agcagtgcgc gttaaaggtc agcgcaatgc gcctgcggct
     1021 gtggaacgcg tcaaccttaa ccgtatcaaa caagaaatga tacgcgacaa taaagacttg
     1081 gtgcgctatt ccaccgatgt cggcttgagc gacagcggcc gccatcaaaa aggctttgct
     1141 gttcgcggcg tggaaggcaa ccgtgtcggc gtgagcatag acggtgtaaa cctgcctgat
     1201 tctgaagaaa actcgctgta cgcccgttat ggcaacttca acagctcgcg tttgtctatc
     1261 gaccccgaac tcgtgcgcaa catcgaaatc gtgaagggcg cagactcttt caataccggc
     1321 agtggtgcat tgggcggcgg tgtgaattac caaacgctgc aaggccgtga tttgctgttg
     1381 gacgacaggc aattcggcgt gatgatgaaa aacggttaca gcacgcgtaa ccgtgaatgg
     1441 acaaatactc tcggtttcgg tgtgagtaac gaccgcgtgg atgctgcttt gctgtattcg
     1501 caacgtcgcg gtcatgaaac cgaaagcgcg ggaaaccgag gctatgctgt ggaaggggaa
     1561 ggcagtggcg cgaatatccg tggttcggca cgcggtatcc ctgattcgtc caaacacaaa
     1621 taccacagct ttttgggtaa gattgcttac caaattaacg ataaccaccg catcgtcggc
     1681 gcatcgctta acggccagca gggacataat tacacggttg aagagtctta taacctgacc
     1741 gcttcttcct ggcgcgaagc cgatgacgta aacagacggc gcaatgccaa cctcttttac
     1801 gaatggatgc ctgattcaaa ttggttgtcg tctttgaagg cggacttcga ttatcagaaa
     1861 accaaagtgg cggcggttaa caacaaaggc tcgttcccga tggattattc cacctggacg
     1921 cgcaactata atcagaagga tttggacgaa atatacaacc gcagcatgga cacccgattc
     1981 aaacgtttta ctttgcgttt ggacagccat ccgttgcaac tcgggggggg gcgacaccgc
     2041 ctgtcgttta aaactttcgt cagccgccgt gattttgaaa acctaaaccg cgacgattat
     2101 tacttcagcg gccgtgttgt tcgaaccacc agcagtatcc agcatccggt gaaaaccacc
     2161 aactacggtt tctcactgtc tgaccaaatt caatggaacg acgtgttcag tagccgcgca
     2221 ggtatccgtt acgaccacac caaaatgacg cctcaggaat tgaatgccga gtgtcatgct
     2281 tgtgacaaaa caccacctgc agccaacact tataaaggct ggagcggttt tgtcggcttg
     2341 gcggcgcaac tgaatcaggc ttggcatgtc ggttacgaca ttacttccgg ctaccgcgtc
     2401 cccaatgcgt ccgaagtgta tttcacctac aaccacggtt cgggtaattg gctgcctaat
     2461 cccaacctga aagccgagcg cagcaccacc cacaccctgt ctctgcaagg ccgcagcgaa
     2521 aaaggcatgc tggatgccaa cctgtatcaa agcaattacc gcaatttcct gtctgaagag
     2581 cagaagctga ccaccagcgg cactcccggc tgtactgagg aaaatgctta ctacggtata
     2641 tgcagcgacc cctacaaaga aaaactggat tggcagatga aaaatatcga caaggccaga
     2701 atccgcggta tcgagctgac aggccgtctg aatgtggaca aagtagcgtc ttttgttcct
     2761 gagggttgga aactgttcgg ctcgctgggt tatgcgaaaa gcaaactgtc gggcgacaac
     2821 agcctgctgt ccacacagcc gctgaaagtg attgccggta tcgactatga aagtccgagc
     2881 gaaaaatggg gcgtattctc ccgcctgacc tatctgggcg cgaaaaaggc caaagatgcg
     2941 cagtacaccg tttatgaaaa caagggctgg ggtacgcctt tgcagaaaaa ggtaaaagat
     3001 tacccgtggc tgaacaagtc ggcttatgtg tttgatatgt acggcttcta caaaccggct
     3061 aaaaacctga ctttgcgtgc aggcgtatat aatgtgttca accgcaaata caccacttgg
     3121 gattccctgc gcggcctgta tagctacagc accaccaact cggtcgaccg cgatggcaaa
     3181 ggcttagacc gctaccgcgc cccaagccgt aattacgccg tatcgctgga atggaagttt
     3241 taatctggta ttattgaatt aatcgccttg ttgaaaatta aagccgtccg aattgtgttc
     3301 aagaactcat tcggacggct ttttaccgta aatctgtgtg atgg
//
LOCUS       DAXHPG010000186         3333 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXHPG010000186 DAXHPG010000000
VERSION     DAXHPG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.186"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..101)
                     /locus_tag="ACFM6F_001946"
     CDS             complement(<1..101)
                     /locus_tag="ACFM6F_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6165303.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVE"
     gene            complement(220..1320)
                     /locus_tag="ACFM6F_001947"
     CDS             complement(220..1320)
                     /locus_tag="ACFM6F_001947"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC6165304.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGTDELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGEKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1645..3219
                     /locus_tag="ACFM6F_001948"
     CDS             1645..3219
                     /locus_tag="ACFM6F_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC6165305.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          680 a          821 c          992 g          840 t
ORIGIN      
        1 tgttcgacgg caaagcgttt gccgccgccg tgggcgggta tgccggtcag tgtgccgcag
       61 gcggttagga cgagggggat gaggaggagc agggttttca tggcagggtt tgttttgttt
      121 tgaacgagtt tgagtgtaaa tggattttaa gggtttgtaa acaaaagggg cgaaaatgcc
      181 gtctgagcgg cggaaatggc tttcagacgg tatttgcgct caataataat atcccgcgcc
      241 cagaatacac ggtttggatg cgccggttgc tttgtgcgga ctaccgggga tgcggttaat
      301 ccaacacgcc gccaaccacg caaatgcggc ggcttctacc cattgcggat cgaggttcag
      361 ttcggcggtg ctgtgcaggg aaacgcgtgt gccgaaacat tctgccaaat ccgccattaa
      421 aacaggattg cggatgccgc cgccgcaaat gtacatttga cgggcatctg ccgctgcgtg
      481 tgagacggcg tcgaaaacgg tttgcgcggt gaatcgggaa agcgtccgca atacgtcgta
      541 tcggttttcg ccgccgtcaa ggtaggtttc gagccaattt agggcaaaca gttcgcgccc
      601 cgtgctttta gggtggggtt gtgcgaaata cgggtgggcg agcagcctgt cgagcagttg
      661 cggcaatatg ttgccttgtg ccgctttttc accgtttttg tcgtaaggca gctgccagtg
      721 tgcctgcgtc cacgcgtcca tcagcatatt gcccggccct gtgtcgaagc cgaaggcggg
      781 tgcgtcgggg gggagtacgc tgatgttggc aatcccgccg atgttcagta ccgcgcgtgt
      841 ttccctgtcg tcgcggaaca gggctttgtg aaaggcgggg acgagcggcg caccttgtcc
      901 gccggccgca aggtcgcggc tgcggaagtc gccgacggta aaaatccgcg tccgttctgc
      961 cagcagcggc aaatcggcaa gctgtatgct gtaaccgtgt tccggcgcgt gtcggacggt
     1021 ttgcccgtgg cagccgaggg cggtaatgtc ggacggcgcg aggttttgac tgcacagcag
     1081 ttcggcggcg gtttgcgcat ataggcggct gagttcttgc gacaaaatcc tgctgcggtg
     1141 cagttcgtct gtgcctgtgt cctgcaaatc cagcaattgg cggcgcagcc tgtcgggata
     1201 gggggtaaag gcgtgccctt ccgcgcccag ccatttgccg ccgtccatcc gtaccagcac
     1261 ggcatccgcc ccatccatac tggttcccga catgatgccg atgtaaagct gtgtttccat
     1321 catcactccc aaactggtgc aaaacgccat tttaacgtgt attgacgctc gtataccgat
     1381 ttgccgccgc agtgtaaata aagtgtaaat aaatgtttca agacggatgg aaaaatatta
     1441 taatgcgccc gcaacatcca gtagtagaag tgtcatacaa accgtttccg gcagcagttt
     1501 tgcattcggt caggtttggg ggtattcgga tgcggttcag aaggaaggat gcgtctgcca
     1561 tatcccgaaa cggcagttcg accggaggca gcagtacagt gtcggcaaca ctcatgattt
     1621 ccaccacatt aaaggaagat tgccatggct caaatccaaa tgagcgcaaa tgttaaaacc
     1681 atcaacgccg tctttgccgc catgctggta ggtacagtcg gctattttat ttattggggc
     1741 ttgggttata cccattacaa ttacgccgcc ttattcatta ttgccacgat gttcggcgtg
     1801 tttatggcgt tcaacatcgg cggcaacgat gttgccaatt ctttcggcac cagcgtcggt
     1861 gcgggtacgc tgaccatccc gcaggctttg ctgattgcgg cggtatttga ggtcagcggc
     1921 gcggtcatcg cgggcggtga ggtaaccaat accatacgca aaggcatcgt cgatttgaag
     1981 ggtgttgatt tcgaacccat acagtttgtg tttattatga tgtccgcgct tttggcggcg
     2041 gcgttgtggc tgttgtttgc ctcgaaaaaa gggcttccgg tatctaccac ccattccatt
     2101 atcggcggca ttgtcggcag cgcggtatgt atggcggtaa tgaacgatgc cgcatcgggc
     2161 gatttgatac gttggggcaa gctgggcggt attggtgttt cttgggtatt gtcgcccgtg
     2221 ttgggcggcg cggtgtccta tttcctgttt tcgcgcgtca agaaaaacgt cttagattac
     2281 aacgcttggg cggaaggcac gctcaagggc atcaagcagg aaaaaaaggc ctataaagaa
     2341 cggcaccgcc tgtttttcga gggtttgtcc gaagccgaaa aagtcgagta cgccaccaaa
     2401 atggcgcacg acgcgcaaat ttacgacgaa cccgaattcg atccgcaaga gctgcaatcg
     2461 gagtattacc gcggtcttta tgcgttcgac aaccgtaaaa acaatgtcga ttcctacaaa
     2521 gcgctgcatt cttggattcc ctttatcgct tcgttcggcg cgatgatgat ttccgctatg
     2581 ctgattttca agggcttgaa aaacctgcat ttggggatga gcaacgtcaa cagcttcctg
     2641 accatcttta tgataggcgc ggcggtgtgg atggggacgt ttgtttttgc caaaagcctc
     2701 aagcgtaaag acttgggcaa atcgaccttt cagatgtttt catggatgca ggtctttacc
     2761 gcctgcggct tcgcattcag ccacggggcg aacgatatcg ccaacgccat cggtccgttt
     2821 gccgcgatta tggatgtttt gcgtaccaac agcgttgccg cgcaaaatgt cgtccccccg
     2881 attgcgatgc tgactttcgg tatcgcgctg attgtcggtt tgtggtttgt cggtaaagag
     2941 gtgattaaaa ccgtcggtac gagtttggcg gaaatgcacc ccgcctcggg ttttaccgcc
     3001 gaactgtccg ccgcatctgt cgtgatgggc gcgtcgctga tggggctgcc cgtgtccagt
     3061 acgcatatct tggtcggcgc ggtactcggt atcggtctgg tcaaccgtaa tgccaattgg
     3121 aaactgatga agcccatcgg tttggcgtgg gtcattaccc tgcctgccgc cgccgtattg
     3181 tcggttgtct gctacttggt tttacaggca gtattctgat tgtaaaatac tgatgccgtc
     3241 tgaacccgtg ttcagacggc attttgttga tggaatgtgc gggcttgtgc cttatgcgca
     3301 atctgttctg tcgggatatg ccgtttggta tag
//
LOCUS       DAXHPG010000187         3317 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXHPG010000187 DAXHPG010000000
VERSION     DAXHPG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.187"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            112..1341
                     /gene="sstT"
                     /locus_tag="ACFM6F_001949"
     CDS             112..1341
                     /gene="sstT"
                     /locus_tag="ACFM6F_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC6165306.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAYTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            1453..2946
                     /locus_tag="ACFM6F_001950"
     CDS             1453..2946
                     /locus_tag="ACFM6F_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6165307.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPVVTE
                     DVGEEVLPKEKKDEEAVSGAPQADTQDATAGKGGQDMAAVSAENTGNGGAATTDNPEN
                     KDEGPQNDMPQNAADTDSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAAD
                     GMQGDDPSAGGENAGNTAAAESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRVDLA
                     NGIKLDSGSENVTLTHCKDKVCDRDFLDEEAPPKSEFESLDDSGRINKYKKDGTSDKF
                     TNLVATEVKANGTNKYVIIYKDKSTSSARVRRSARSRRSLPAEMPLIPVNQADTLIVD
                     GEAVILTGHSGNIFAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGE
                     VLHFHTENGRSYPTKGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAVIDGNGFKGT
                     WTENGGGDVSGRFYGPAGEEVAGKYSHRPTDAEKGGFGVFAGKKEQD"
     gene            complement(3021..3236)
                     /locus_tag="ACFM6F_001951"
     CDS             complement(3021..3236)
                     /locus_tag="ACFM6F_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165308.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(3229..>3317)
                     /locus_tag="ACFM6F_001952"
     CDS             complement(3229..>3317)
                     /locus_tag="ACFM6F_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6165309.1"
                     /translation="VTMEKDLRRMLLFFKREAYVVILERDRV"
BASE COUNT          800 a          810 c          967 g          740 t
ORIGIN      
        1 tgatttttgt taatccacta taaaacacag ccgaaaaata aaaaaacaca gagaatgagt
       61 tttcttttaa aatacaacat tttttaacat atcacaacat aaggaagaat tatggcgttc
      121 ggcaaatcct tatttcatgc gatcggccgc gtcagcctgg tcaggcagat tgccgccggt
      181 ttggcgttgg gcatcgtaat cggttcggtt tccccgcaac tgggcttggc ggcaggcttg
      241 ttcggcagcc tgtttgtcgg cgcgctcaaa gcggtcgcgc cggttttggt atttattttg
      301 gtggcggcca caatcgcgca gcaccaaaaa ggcaacaagg cgcatatcag gccgattatc
      361 gtcctttacc tcatcggtac gttttccgca gccctgaccg ccgtcatcgc cggtatggtt
      421 ttcccgacgc acattgtttt ggcgggcgcg ggcgatgtgt ccgccgcgcc gccttccggc
      481 attgtggaag tattgaaatc gctgctgatg aacctggtcg ccaacccgat taacgcgatt
      541 gccaatgcca actatatcgg cattttggct tgggctttgg ttttgggcgc ggcgttgcgg
      601 aatcacggtt cggacgttac gcggcaggtc gttgccgatt tggcggaagc ggtttccacc
      661 gtcgtgaaat ggattatccg ttttgcccct ttgggcattt tcgggctggt gtcgtccaca
      721 atcgcggaga cgggtttcgg cgcgctggcg ggttatgcga agctgcttgc ggtactgttg
      781 ggctgtatgg cgtttatcgc gctggtggtc aatcccgcca tcgtgtggtg gaaaatccgc
      841 cgcaaccctt atccgctggt gtttacctgc ctgcgcgaaa gcggcgtgta tgccttcttt
      901 acccgttctt ccgccgccaa tatccccgtg aatatggctt tggcgaaaaa actggggctg
      961 cacgaagaca cttattcgat ttccatcccg ttgggggcga ccgtcaatat gggcggcgcg
     1021 gcgattacga ttaccgtttt ggctatggcg gcggcgtaca cccaaggcat acaggttgat
     1081 tttgccaccg cgctgctgtt gagcctggtg gcgacggtca gcgcgtgcgg cgcgtccggt
     1141 gtggcgggcg gttcgctgct gctgattccg ctggcgtgca gcctgttcgg catcagcaac
     1201 gatgtcgcta tgcaggtggt cgccgtcggc tttatcatcg gcgtgattca ggattcggcg
     1261 gaaacggcgt tgaattcttc aaccgatgtt ttgtttaccg ccgccgccga tttgggcaga
     1321 cagagaaacc gggcggaata aaccaagcta tgccgtctga agcccttttg gcgttcagac
     1381 ggcatatttt tacaatttta tgggcatatg gcggaaacaa taccggctgc cttgaatatg
     1441 aaggagatga tgatgtttaa acgcagtgtg attgcaatgg cttgtattgt tgccctttca
     1501 gcctgtgggg gcggcggtgg cggatcgccc gatgttaagt cggcggacac gctgtcaaaa
     1561 cctgccgccc ctgttgttac tgaagatgtc ggggaagagg tgctgccgaa agaaaagaaa
     1621 gatgaggagg cggtgagtgg tgcgccgcaa gccgatacgc aggacgcaac cgccggaaaa
     1681 ggcggtcaag atatggcggc agtttcggca gaaaatacag gcaatggcgg tgcggcaaca
     1741 acggataatc ccgaaaataa agacgaggga ccgcaaaatg atatgccgca aaatgccgcc
     1801 gatacagata gttcgacacc gaatcacacc cctgcaccga atatgccaac cagagatatg
     1861 ggaaaccaag caccggatgc cggggaatcg gcacaaccgg caaaccaacc ggatatggca
     1921 aatgcggcgg acggaatgca gggggacgat ccgtcggcag gcggggaaaa tgccggcaat
     1981 acggctgccg ccgaatccgc aaatcaaaca gggaacaacc aacccgccgg ttcttcagat
     2041 tccgctcccg cgtcaaaccc tgcacctgcg aatggcggca gcgattttgg aagggttgat
     2101 ttggctaatg gcatcaagct tgacagcggt tcggaaaatg taacgctaac ccattgtaaa
     2161 gacaaagtat gcgatagaga tttcttagat gaagaagcac cgccaaaatc agaatttgaa
     2221 tcgttagatg actctgggcg aattaataaa tacaagaaag atggaacaag cgataaattt
     2281 actaatttgg ttgcgacaga agttaaagct aatggaacta acaaatatgt catcatttat
     2341 aaagacaagt ccacttcatc tgcgcgagtt aggcgttctg cacggtcgag gaggtcgctt
     2401 cccgccgaga tgccgctaat ccccgtcaat caggcggata cgctgattgt cgatggggaa
     2461 gcggtcatcc tgacggggca ttccggcaat atcttcgcgc ccgaagggaa ttaccggtat
     2521 ctgacttacg gggcagaaaa attgtccggc ggatcgtatg ccctcagtgt gcaaggcgaa
     2581 ccggcaaaag gcgaaatgct tgctggcacg gccgtgtaca acggcgaagt gctgcatttc
     2641 catacggaaa acggacgttc gtacccgacc aaaggcaggt ttgccgcaaa agtcgatttc
     2701 ggcagcaaat ctgtggacgg cattatcgac agcggcgatg atttgcatat gggtacgcaa
     2761 aaattcaaag ccgttatcga tggaaacggc tttaagggga cttggacgga aaatggcggc
     2821 ggggatgttt ccggaaggtt ttacggcccg gccggcgagg aagtggcggg gaaatacagc
     2881 catcgcccga cagatgcgga aaagggcgga ttcggcgtgt ttgccggcaa aaaagagcag
     2941 gattgatgtt gatgccgccc gaagcggggg cgttcagacg gcattgtgat gttttgggtg
     3001 gggtccggtt caagtccgca tcagttgata aacccgtcgt ctttcatttt cttttcggaa
     3061 aaatactggc cgaataccag cagggcggcg aggatgatga caaacagccc cgccatagtt
     3121 agtgtgattt tgaagaacaa ttccgcttcg agaaaagaaa accagagttg cacaatcagc
     3181 agcagcaccc agccggcaaa cagtccgaga caggcataaa cgccgagctt aaacacggtc
     3241 ccgctccaaa atgacgacgt aggcctcgcg tttgaaaaac agcagcatac ggcgcaaatc
     3301 tttttccatc gttacga
//
LOCUS       DAXHPG010000188         3228 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXHPG010000188 DAXHPG010000000
VERSION     DAXHPG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.188"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(126..509)
                     /locus_tag="ACFM6F_001953"
     CDS             complement(126..509)
                     /locus_tag="ACFM6F_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165310.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(514..1351)
                     /locus_tag="ACFM6F_001954"
                     /pseudo
     CDS             complement(514..1351)
                     /locus_tag="ACFM6F_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase domain-containing protein"
     gene            complement(1650..2045)
                     /locus_tag="ACFM6F_001955"
     CDS             complement(1650..2045)
                     /locus_tag="ACFM6F_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165311.1"
                     /translation="MNQFCLSEYEKAFLCRIIENNMIWKEYLIEQIENSIIVSRDNTG
                     CGCYIDFQVNLERTNLNKYENIPIFFEGDITAPDKSDAATFLLYTTESLNKAGRIIDF
                     LEVAIAGTNYDEVEVYSWLKMENSLRFGL"
     gene            complement(2057..2770)
                     /locus_tag="ACFM6F_001956"
                     /pseudo
     CDS             complement(2057..2770)
                     /locus_tag="ACFM6F_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(2866..3138)
                     /locus_tag="ACFM6F_001957"
     CDS             complement(2866..3138)
                     /locus_tag="ACFM6F_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165312.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
BASE COUNT          948 a          656 c          519 g         1105 t
ORIGIN      
        1 agcacacacc ttacgcatgg attttaggtt tcatgcaggc tacagcttgc tgctgcggta
       61 taaaagtatt tttagtgatt tactgttttt ggtatttttg cccgacgggg ttaaaaatac
      121 agttgctact cttgctgcat gatattggca agatttaatt taaatgtttg ttgaatcttg
      181 tttacatcct tagcaaaaat atgatagtca ctcgaagaat agatagattt tattctatcc
      241 aaattagttt gttgataaca ttttttcatt tccgcgatga aaaaattatc ttttcctctt
      301 tcattgagac agttattttc taacagatag attgcccatg ctgtaaaaat aaatgtacta
      361 tcttccggat attcagataa ataaatttcg aattcaatta tagaatattc agctgcttca
      421 attaatgtta tatctaaatc cctgacaaat ttttccgtta tatttttaaa taatttcttg
      481 aattctatat aattttttat tttttccatg attctatttt cctgtaaaaa tatttaattg
      541 aatatttgga tagcgatttc taaactctaa aataacattg ctgcaagatt gacaggcctt
      601 taattctgta aatagatcaa ttctacctga tacattacga ttatttccga gttgctgtgc
      661 tatagtttca agtaatttat attctccatc tatatttctt aatgttcctc taggaggaga
      721 ggcaatatta tgatatttat caacagatat aggtttaaaa atttttgttt caggtaacga
      781 aataaatcca tgttcccctt tttggaaaga actaaatgct tccatccttt gcggtaatcc
      841 tttaacatca atttttgcta tcgcaagatt ccctatttgt cgatactctt ccggcagttt
      901 catgcggact ctctggcttt cagcagttaa aagcaggagc actgtcattg ctcagataac
      961 gggtaacagg tgtaggcttg gccgatattg cagcctgtgc agatacagat cctgcaacca
     1021 tcaatgtgcc attaacaacc tgaactgtac catgcagata ttctccatta gagattgctt
     1081 tcgctccctg tccaatgtta tatccccctg ctgccacagt agctgctttg acactgaagc
     1141 caagcggtcc gtttaataag taaagtgcag gttgtgcggc aacagctgat ttgtaatcaa
     1201 aagaatcata acgactcaat gtttgatagg ctttcgataa agtgttatta atatctttgg
     1261 aaggaatagt gagattaccc attgactggg gcgttttcag tatcgaagct gcccaagctg
     1321 tgttacctcc agaggcttgg ttaataaaat atgttaccta tgtatattgt ttcttcaaaa
     1381 atcaaccgta ttgtccttgg gtgattttcg taccagcaca atcagtctgc cctagttgac
     1441 agatcgcgcc acactagcca gatacagcgt atcattggta aaactgataa tgcggcaata
     1501 taaagaaaaa cttcaatagt atgttaacga aatttgacgc ataaaaatac gctaaaaaat
     1561 tttcgcttga ctaattaaaa atgggattga gcataaaatt ttaataattt atattattga
     1621 aaatatctca gattttcagt cagtttattt tacaagccga accgtaggct attttccatc
     1681 tttaaccatg aatagacctc aacttcatca taatttgtgc ctgcaatagc aacttccaga
     1741 aagtcaataa ttctacctgc tttattcaaa gactctgtag tatatagcag gaaagtcgct
     1801 gcatctgact tatccggtgc agtgatgtct ccttcaaaaa aaatagggat attttcgtat
     1861 ttatttagat tagttctttc taaatttacc tgaaaatcaa tgtagcaccc acatcctgta
     1921 ttatctctag atactattat tgaattttct atttgctcta ttaaatattc cttccatatc
     1981 atattgtttt caataattct acataagaaa gccttctcat attcagataa acaaaactgg
     2041 ttcatatatt ctacccttac attttcaaaa tagttgcaat acgagatact ccattgttga
     2101 catatacgcg aacaattacc gttataccat tgaccgttac agtaactgga taatttcctg
     2161 tttttgtcag ttttaaagca tccactgcct tttgcgtcgc tacaacagca ttttccatgg
     2221 agcctgattt ttgaatcaat gtattaccga tgcttccttg atgcccctta aagatgtgat
     2281 gaactttatt tagccaatct acaccgtatc tttcagccaa tagtcgagaa ttactttcac
     2341 ctattttttc catcgaagct aaaatccttt gagcataggc agcatcagct gttgaaaaat
     2401 ttgcttcgcc caacaaaagc cgtaattcct gaatatctcc tcgtaaaatt gcatttctta
     2461 ctcccgcttc taatgtggtt tgtggctgaa agagggaatt attctctacc gccacagtag
     2521 ccgcattcgc cgctgtattc acatccccgc ctacaacggc agacgcagtg ccggcaacca
     2581 gttttgaata ggcagtaatc ttcgctttag ctttttcgat ttcggtcgca ctcatacgac
     2641 tgaaatcagt attcttaacc aaagcctcac caacaatctc accgactgca gcgcctatcg
     2701 caccatcctg acacttaccc ttattcgccg ccgctgccgc acagcccgct atggcatggg
     2761 caatcttgtg ggtggtatat cgctgccagc cctctctgtt gataatcttg cgtatgatta
     2821 cgccgtttga tggctaatcg tacctacgca ggcttaaaat ttccatcaat ccatatcatt
     2881 tgagttttca tcttcataat atggatgatc aaacaaatct ccttcccaaa tacattcact
     2941 cgttatacct tgggattcca tgaaaggaac ataagaataa aaatcttttt catattctcc
     3001 ataaaatata ttcatacctt tgagccatac tgcatcgcat tcatcacaca gaataatttc
     3061 atcatgaaga tatttaggtt tcgaatggta gagatagcct tgttgacaag caggacacat
     3121 tttcattctc ttattcatga ttactccata taatcgattt atttttgtgg gtaagcagta
     3181 gtgatttgat tgctgccttt agtctgtaca acaatacgta tatagcaa
//
LOCUS       DAXHPG010000189         3175 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXHPG010000189 DAXHPG010000000
VERSION     DAXHPG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.189"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            52..1005
                     /locus_tag="ACFM6F_001958"
     CDS             52..1005
                     /locus_tag="ACFM6F_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC6165313.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="ACFM6F_001959"
     CDS             complement(1116..1439)
                     /locus_tag="ACFM6F_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165314.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="ACFM6F_001960"
     CDS             complement(1612..2973)
                     /locus_tag="ACFM6F_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC6165315.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
BASE COUNT          746 a          931 c          766 g          732 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgta
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 aacgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctctg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gctgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgtttcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 tagactttat ttacggggat gaatcccctt acaaatcagg ataatacata gaacggcact
     1561 ttatgcttca tcccatgtgt tgaaacttca ttgctccttg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggacgt gcgcctttgc gattttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gcacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcacgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagggccgc cagtacgaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaacg cgcaaggcat tgagcagcgg atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttc agacactcgc gcgcgtgccc tgaaaacagc agtttcataa tttcgtcgaa
     2281 cagccgcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggcggt
     2341 ttcttccgac agatcaaagc ccaatttgct cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc accaatcata accagcctgc gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tcaggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccgtagg tgttgtcctt
     2581 catgattctg ccgtgcgcgt tctgatgaac cttcgcaccg ccgcgaaacg tcgttacttc
     2641 gataatctcc gcaccgttca tcacatggac aatctgaaaa cgcctgccga tgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg cgtggcatcg gttgcgacat cgaaatcctt
     2761 gggttcgatg ccgagcagca ggtccctgac cgcgccgccg accacataag cctgaaaccc
     2821 cgcccctttc aggcggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcaggaa tgattgtttt actttccgct tttttactgc tccgaccgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctaacccgaa gagcggcatc
     3001 tccacgggca accgtgttca gacggcatca ggcggtacga ttccgcaaga tgccgtaacc
     3061 ataaacaaaa ggcgtagaaa ctacgccgtc cgaatcacgc cgccctcgcg gcggcggcaa
     3121 cgcgccatta taacagattg ccctttaaaa aaatcagacc ctgcttttgt ggcgg
//
LOCUS       DAXHPG010000190         3152 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXHPG010000190 DAXHPG010000000
VERSION     DAXHPG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.190"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            212..991
                     /locus_tag="ACFM6F_001961"
     CDS             212..991
                     /locus_tag="ACFM6F_001961"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6165316.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSLRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKFTLES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(1191..2576)
                     /locus_tag="ACFM6F_001962"
     CDS             complement(1191..2576)
                     /locus_tag="ACFM6F_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFC6165317.1"
                     /translation="MIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYEDRVTEVALV
                     KFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELFSVLKGCVLV
                     AHNSRFDYTFLKHEFHRVGIGFSSPALCSVQLSRCLYPQFYKHSLDSIIERLGIVVED
                     RHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLREQLYGLPDG
                     MGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFPALGSLHAYK
                     IKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVTDNPPTGLFA
                     NKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACGRSDEQVLAF
                     AHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKDLPDAFKAKF
                     KTDRKNIKEIG"
     gene            2694..2770
                     /locus_tag="ACFM6F_001963"
     tRNA            2694..2770
                     /locus_tag="ACFM6F_001963"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2728..2730,aa:Arg,seq:acg)
     gene            2788..2862
                     /locus_tag="ACFM6F_001964"
     tRNA            2788..2862
                     /locus_tag="ACFM6F_001964"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2821..2823,aa:Glu,seq:ttc)
BASE COUNT          859 a          937 c          618 g          738 t
ORIGIN      
        1 gtccggatgc ctgattatcg ggtatccggg gaggattaag gggatatttg ggtaaaatta
       61 ggaggtattt ggggctaaaa cagccgaaaa cctgtgtttg ggtttcggct gtcgggaggg
      121 aaaggaattt tgcaaaggtc tcagaccgtc aatgcggaaa aaaacaccca aattggctat
      181 aatcccgaca aacacactca aggacaacaa catggcagcc tcgcccgaag caaaattcac
      241 tgaagaaaag attttgtggg tcaaacacca cacgccgaaa ctcatcactt tcgccatcag
      301 ccgtcccgaa tcctaccgtt ttaaagccgg acagttctcc cgactcggtt tctacgaagg
      361 ggaaggcttt atttggcgtg cctactccat cgtttccgca gaatatgccg acacgctcga
      421 atattttgcc gtactcatcc aagacggccc catgtcggcc cgtttcgcca aaatgcaaca
      481 gggcgacacc atcctgctcg ataaaaatgc caccggcttc ctcctgcccg aacgcttccc
      541 cgacggcaag gatttggtaa tgctctgcac cggctccggc atcgccccct tcctttccat
      601 tctcgaacaa cccgaaatcc ggcagcgttt cgataccgtc aacctgatac attccgtatc
      661 ttttcccgaa gaattgattt tcaacgaccg gctcgccgca ttgtccgaac atcccctggt
      721 aggtgaatac ggacactcgc tccgtttcgt ccctgttacc acccgtgccg ccaacccctc
      781 gggcttaagc ggaaaacgca ttccggaact cttaaaaaac aacagcatcg aacaggcact
      841 gcataccaag ttcaccctgg aatccacacg gtttatgatt tgcggcaacc cggaaatggt
      901 caaagacact ttccaaacgc tgcttgacat gggttacgcc atgcaccgca accgcattcc
      961 cggtcaaatc atgatggaaa acggcttcta aaaaccaccc tgcttgtccg atgcctcgga
     1021 cgggcaaacc ggcacgacac gcaaaccgcg tcggcaaaaa tgccgtctga agccttcaga
     1081 cggcattttc ggatacatac aaacggcaac atattccaac ccaaaccgat taggcaatca
     1141 atacgaacgg ctgtttacat acttaccggc tttcaccaac cggtatcgat ttaaccgatt
     1201 tccttaatat ttttcctgtc cgttttaaac ttcgccttaa acgcatccgg taaatcttta
     1261 tcgaaatacc aaagcccgtc acccatttcc aatgcgccgc ccattccatg cagaacaact
     1321 ttttcccccg ttaaaggatc agtttcggta atttctacct catgcatttt tccccaatcc
     1381 aaaataggca acttgtgtgc aaacgccaaa acctgttcgt cagaacggcc gcacgcctta
     1441 tcgcatcggc ctgaaacata tacgggacaa ggctcatcct ctgcataacc gtccggaagg
     1501 ataccggctg aggcaggaca caatccgtat gtttccgccc acgacgacaa agcccgtctc
     1561 gccgcctttt tattggcaaa taatccggta ggcggattat ccgtcacacc gtttttcaaa
     1621 gccgctgttt tcgcattcaa catgccgtct gaaccttttt caaacctgac ggtcgtaaat
     1681 gttttcagca gatttttggc agatacataa caacccgaat gataacgccc gaccaattcc
     1741 gctttaatct tatatgcatg caggctgccc aaagcgggaa aaaaacggac ttcctccgta
     1801 ttgcaccaat caaacggggc ttttccggaa tccaataaag ccgaaacctc gctatatacc
     1861 cgttcaaacg taccgatgta atttactttc cctccgccgt cgaaacaagc cagcaccccc
     1921 ataccgtcag gcaaaccgta caactgttcc ctcaaccgtt cgggcagcgc ggcaggcaac
     1981 ggtttcggat tcatcaaacg gaaacactgc ctgatccatg cctcgacccc gtgttccgac
     2041 agactgtatt ccaaataatc gcacaatgcc gatacatccg ccatcgcacg atgcctgtct
     2101 tccacaacaa tccccaacct ttcgatgata ctgtccaggc tgtgcttgta aaattgcgga
     2161 tacagacacc gggacagctg cacactgcac aaagcaggcg atgaaaatcc gatacccaca
     2221 cgatgaaact catgctttaa aaacgtatag tcgaaacggc tgttatgtgc aaccagcaca
     2281 caacccttca ataccgaaaa caactcgccg gcaatctctg caaaaacagg cgcatcggca
     2341 accatgccgt ctgaaatccc cgtcagcccc gccacaaact gcggaatcgg tttttgagga
     2401 ttaaccaacc actcatgcct caccaccctt ccctgctcaa acttgaccaa agccacttcg
     2461 gttaccctgt cttcatacag attgccgccc gtcgattcca aatcaaccac ggcaaccggc
     2521 attccaaacc gtaaaaatac cttttccagc aagggccagc gagaagcaac aatcatttta
     2581 ttctctttaa attcaaacaa caaaccgata ttttacactt ttgaggcatt tcatccaaca
     2641 aaacaattga cagaatccga tgattaccct aaaattcaaa tctttcttgc agcgcacccg
     2701 tagctcagtt ggatagagta tctggctacg aaccagaggg tcgggcgttc gaatcgctcc
     2761 gggtgcgcca gtaagaaagt acaatatgcg cccatcgtct agcggttagg acatcgccct
     2821 ttcacggcgg taaccggggt tcgattcccc gtgggcgtgc caaattccaa atccccgaga
     2881 ttatccctcg gggatttttt attgtctcgg caactcgtta ccatatcttt acctaccccc
     2941 ttcatcagaa tctcagacac aatcgaatca tattcaaacc tttgccgtgc aaaccgatat
     3001 cccataaccg gatgcggtgt ccgtccaaca ttttacccga ttgaaacgcc tgatatatcg
     3061 caccccatca aaggggcatt gcttttctta acaatcccct ttgacagggc ttttttatgc
     3121 catcatcaaa tttatagtgg attaacttta aa
//
LOCUS       DAXHPG010000191         3102 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXHPG010000191 DAXHPG010000000
VERSION     DAXHPG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.191"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..212)
                     /locus_tag="ACFM6F_001965"
     CDS             complement(<1..212)
                     /locus_tag="ACFM6F_001965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165318.1"
                     /translation="MLPTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQA"
     gene            272..895
                     /locus_tag="ACFM6F_001966"
     CDS             272..895
                     /locus_tag="ACFM6F_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC6165319.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1006..2388
                     /locus_tag="ACFM6F_001967"
     CDS             1006..2388
                     /locus_tag="ACFM6F_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6165320.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSIITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2392..2916
                     /locus_tag="ACFM6F_001968"
     CDS             2392..2916
                     /locus_tag="ACFM6F_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC6165321.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          618 a          838 c          945 g          701 t
ORIGIN      
        1 gcctgctgcc gcccgaaaag cgtgatgcgc tgatggtgct gctgcataat ccggacgacg
       61 gataaccggt gaaaacggaa atgcccgggc ggtgttgcct gtgcctgttt ggacaagcgt
      121 tcttgagggc ggtagaattg aggtttgccc gaaaagggca gggcgatatg ccgcgcttgg
      181 attttgcgcg cggtttttcg cgttggaagc aatcattgtc tgtttgaata tttaatgccg
      241 tctgaaacgt ccggacgcgc ccccgttccc tatgaaaaaa ccgaccgata ccctacccgt
      301 taatctgcaa cgccgccgcc tgttgtgtgc cgccggtgcg ttgttgctca gtcctctggc
      361 gcacgccggc gcgcaacgtg aggaaacgct tgccgacgat gtggcttccg tgatgaggag
      421 ttctgtcggc agcgtcaatc cgccgaggct ggtgtttgac aatccgaaag agggcgaacg
      481 ttggttgtct gccatgtcgg cacgtttggc aaggttcgtc cccgaggagg aggagcggcg
      541 caggctgctg gtcaatatcc agtacgaaag cagccgggcc ggtttggata cgcagattgt
      601 gttggggctg attgaggtgg aaagcgcgtt ccgccagtat gcaatcagcg gtgtcggcgc
      661 gcgcggcctg atgcaggtta tgccgttttg gaaaaactac atcggcaaac cggcgcacaa
      721 cctgttcgac atccgcacca acctgcgtta cggctgtacc atcctgcgcc attaccggaa
      781 tcttgaaaaa ggcaacatcg tccgcgcgct tgcccgcttt aacggcagct tgggcagcaa
      841 taaatatccg aacgccgttt tgggcgcgtg gcgcaaccgc tggcagtggc gttgattttg
      901 aacccgcgcc gcaaccgaaa tacggcgaat cctgtataat ccgaaaatct gttcactgga
      961 agttcagacg gcattgcaac tgttgatgcc gtctgaaaaa atatgatggc aagagacaac
     1021 cgcatccaaa tgtttccgca cgaatggcgc gccagtacga cgctttccgg cgtgtacgcg
     1081 ctgcgtatgc tgggtatgtt cctcgtgctg cccgtattgg cggtgtatgc cgcctcgctg
     1141 cccggcgcgg aaggcaacaa aacgctggtc gggctggcaa tgggcattta cgggctgaca
     1201 caggctctgc tgcaactgcc tttgggcatc gcttccgaca agttcgggcg caagaaaacc
     1261 atttatgtcg ggctgattgt gtttgcagcg ggcagcttcc ttgccgccgc cgccgatacg
     1321 ctgcccatgc tggtcgccgc acgcgccata cagggtgcgg gagcggtcag tgcggcggtt
     1381 accgcgctgc tggcggattt gacgcgcgac ggagtacgta cccgcgcgat ggcgatgatc
     1441 ggtttgagta tcggtctgac gttttcggtc agcctcgtcg ttgcccctgt gattgccgac
     1501 gcggtgggcg tacgcggctt gtttctgctg accggtattt tgaccgttgc cagtatcggc
     1561 gtggtggcgt ggatgactcc cgacccggag gtttccaagc tgcacgaaga tacgcaggcg
     1621 cagccttcgc gcataggcga agttttgaaa aaccgtaggc tgctgacgct cgatttcggc
     1681 attttcgccc tgcacgccgc gcaaatggca ttgtttaccg cgctgccttt tgcgatgacg
     1741 cagctcggtt tggaaaaaat ccagcattgg aaagtctatc tgccctcgat cattacgggc
     1801 ttggtggtga tggttccgct gattatcgtc ggcgagacgc gcaacaagct caaacaggtt
     1861 tttgttttgg gtatcgtctg tattgcggcg gcgcagctcg gtttgctgtc cggtatgcgc
     1921 tcggtaggct tgattaccgc ttatttggtt gtttacttta tcggttttaa tgtgttggaa
     1981 gcgagcctgc cgtctatggt ttccaaaatc gcgccgtccg acctgaaggg tacggcgatg
     2041 ggtgtgtaca acacgatgca gtcgctcgga ctgtttgccg gcggcgcggc aggcggcttg
     2101 ctgtttcaaa aatacggctt ttccggcgtg tttgcctttt gcagcatatt gatgctgctg
     2161 tggctggtaa ttgccgtttt atcgcctgcg cccaagcccg tcaaaaacct cagttaccct
     2221 gtcggcggcg tgtggcaggg caatcgggac ggtttgcaac gcgccttgtc ggagcttgag
     2281 ggcgtggaag acatcggttt cagtttcgac gggcagaccg tctatcttaa agtgttgcag
     2341 aagggtttcg atcaggctgc cgctgaaaaa atcatcacag gagtttaaaa aatgtcattg
     2401 aacaaagtca tcctcatcgg ccgcctcgga cgcgatcccg aagtgcgcta tatgcccaac
     2461 ggcgaggcgg tgtgtaattt cagcgtcgcc accagcgaaa cttggaacga ccgcaacggc
     2521 caacgtgtag agcgtactga gtggcacaac atcacgatgt accgcaaact ggcggaaatt
     2581 gccgggcaat acctcaaaaa aggcgggctg gtttatctgg aaggcagaat ccaaagccgc
     2641 aaataccaag gcaaagacgg catcgaacgc accgcttacg atattgtcgc caacgaaatg
     2701 aaaatgttgg gcgggcgcaa tgaaaacagc ggcggtgcgc cttacgagga aggttacggt
     2761 cagagtcagg aggcttacca acgccccgcg cagcaaagcc ggcagcccgc ctccgacgcg
     2821 ccgtcccatc cccaagaagc accagccgcg ccgcgccgcc aacccgtgcc tgccgccgcc
     2881 ccggtcgagg acattgacga cgatatcccg ttctgaattt tacggccgga catcctcgcg
     2941 gagggaaatc ataaaggacg gagaaacctt aaaccttacg gggcagggtt tctctttttt
     3001 gcatttgggc cggcttgttg ccgccctttg cgttgggcgg aacgttgcga ttgcgccgtt
     3061 cgggcgtttt gaggcagtga tgcctgccgc acgtccccgc tg
//
LOCUS       DAXHPG010000192         3066 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXHPG010000192 DAXHPG010000000
VERSION     DAXHPG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.192"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            860..3004
                     /gene="fetA"
                     /locus_tag="ACFM6F_001969"
                     /gene_synonym="frpB"
     CDS             860..3004
                     /gene="fetA"
                     /locus_tag="ACFM6F_001969"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC6165322.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNNEKDYEAGKGFRNNFNGGK
                     TVPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFTVGSEDSRINIK
                     RQAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPN
                     HTRITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSQFKIEDKEKATDEEKN
                     KNRENEKIAKAYRLTNPTKTDAGAYIEAIHEIDGFTLTGGLRYDRFKVKTHDGKTVSS
                     SNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKAERARN
                     TEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIKNHGYELGAS
                     YRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFQNPNLEIG
                     WRGRYVQKAVGSILVAGQKDRNGKLENVVRKGFGVNDIFANWKPLGKDTLNVNLSVNN
                     VFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          805 a          875 c          705 g          681 t
ORIGIN      
        1 aagcaccaag tgaatcggtt ccgtcctatc cgtactgtct gcggcttcgc cgccttgtcc
       61 tgatttccgt tcgttttcgg ttattcccga taaattaccg ccgtttctca tcatttcttt
      121 aacccttcgt cattcccgcg caggcgggaa tccagtctgt tcggtttcgg ttttttccga
      181 tagattcctg ccgcgttagg ggttctagat tcccgctttc gcgagaatga cgcggaaagg
      241 ttgcttttcg tcattcccgc gcaggcttcg tcattcccgc gcaggcggga atccagtctg
      301 ttcggtttcg gttttttccg atagattcct gcccgttagg ggttctagat tcccgctttc
      361 gcgggaagga cgcggaaagg ttgcttttcg tcattcccgc gcaggcctcg tcattcccgc
      421 gcaggcggga atctaggttc gttcggtttc ggttttttcc gatagattcc tgccgcgtta
      481 ggggttctag attcccgctt tcgcgggaag gacgcggaaa ggttgctttt cgtcattccc
      541 gcatttgtta atccactata tttccgccgt tttttatagt ggactaacaa aaaccagtac
      601 agcgttgcct cgccttgccg tactatctgt actgtctgcg gcttcgtcgc cttatcctga
      661 tttttgttag tccactatac atttccgaca aaacctgtca acaaaaaaca acacttcgca
      721 aataaaaacg ataatcagct ttgcaaaaat ccccccccct gttaatataa ataaaaataa
      781 ttattaatta tttttcttat cctgccaaat cttaacggtt tggcttaact tcccttcata
      841 cactcaagag gacgattgaa tgaatacccc attgttccgt ctcagcctgc tctcgcttac
      901 cctagcggca ggttttgccc atgcggcaga aaataatgcc aaggtcgtac tggataccgt
      961 taccgtaaaa ggcgaccgcc aaggcagcaa aatccgtacc aacatcgtta cgcttcaaca
     1021 aaaagacgaa agcaccgcaa ccgatatgcg cgaactctta aaagaagagc catccatcga
     1081 tttcggcggc ggcaacggca cgtcccaatt cctgacgctg cgcggcatgg gtcagaactc
     1141 tgtcgacatc aaggtggaca acgcctattc cgacagccaa atcctttacc accaaggcag
     1201 atttattgtc gatcccgctt tggttaaagt cgtttccgta caaaaaggcg cgggttctgc
     1261 ctctgccggt atcggcgcaa ccaacggcgc gattatcgcc aaaaccgtcg atgcccaaga
     1321 cctgctcaaa ggcttggata aaaactgggg cgtgcgcctc aacagcggct tcgccagcaa
     1381 cgaaggcgta agctacggcg caagcgtatt cggaaaagag ggcaacttcg acggcttgtt
     1441 ctcttacaac cgcaacaatg aaaaagatta cgaagcaggt aaaggcttcc gtaataattt
     1501 caacggcggc aaaaccgtac cgtacagcgc gctggacaaa cgcagctacc tcgccaaaat
     1561 cggaacaacc ttcggcgacg gcgaccaccg catcgtgttg agccatatga aagaccaaca
     1621 ccgaggcatc cgcactgtgc gtgaagaatt taccgtcggc agcgaagatt cacggataaa
     1681 tattaaacgc caagcccctg cttaccgcga aaccacacaa tccaacacca atttggcgta
     1741 cacgggtaaa aacctgggct ttgtcgaaaa actggatgcc aacgcctatg tgttggaaaa
     1801 agaacgctat tccgccgatg acagcggcac cggctacgca ggcaatgtaa aaggccccaa
     1861 ccatacccga atcaccactc gtggtgcgaa cttcaacttc gacagccgcc ttgccgaaca
     1921 aaccctgctg aaatacggta tcaactaccg ccatcaggaa atcaaaccgc aagcgttttt
     1981 gaattcacaa tttaaaattg aagataaaga aaaagcaact gatgaagaga aaaataagaa
     2041 ccgtgaaaat gaaaaaattg ccaaagccta ccgtctgacc aacccgacca aaaccgatgc
     2101 cggcgcgtat atcgaagcca ttcacgagat tgacggcttt accctgaccg gcgggctgcg
     2161 ttacgaccgc ttcaaggtga aaacccacga cggcaaaacc gtttcaagca gcaaccttaa
     2221 cccgagtttc ggcgtgattt ggcagccgca cgaacactgg agcttcagcg caagccacaa
     2281 ctacgccagc cgcagcccgc gcctgtatga cgcgctgcaa acccacggca aacgcggcat
     2341 catctcgatt gccgacggca ccaaagccga acgcgcgcgc aataccgaaa tcggcttcaa
     2401 ctacaacgac ggcacgtttg ccgccaacgg cagctacttc tggcagacca tcaaagacgc
     2461 gcttgccaat ccgcaaaacc gccacgactc tgtcgccgtc cgtgaagccg tcaacgccgg
     2521 ctacatcaaa aaccacggtt acgaattggg cgcgtcctac cgcaccggcg gcttgactgc
     2581 caaagtcggc gtaagccaca gcaaaccgcg cttttacgat acgcacaaag acaagctgtt
     2641 gagcgcgaat cctgaatttg gcgcacaagt cggccgcact tggacggctt cccttgccta
     2701 ccgcttccaa aacccgaatc tggaaatcgg ctggcgcggc cgttatgttc aaaaagccgt
     2761 gggttcgata ttggtggcag gtcaaaaaga ccgcaacggc aaattggaaa acgttgtacg
     2821 caaaggtttc ggtgtgaacg acatcttcgc caactggaaa ccgctgggca aagacacgct
     2881 caatgttaat ctttcggtta acaacgtgtt caacacgttc tactatccgc acagccaacg
     2941 ctggaccaat accctgccgg gcgtgggacg tgatgtacgc ttgggcgtga actacaagtt
     3001 ctaaaacgca catcccgaaa aaatgccgtc tgaaagcctt tcagacggca tctgttctga
     3061 taattt
//
LOCUS       DAXHPG010000193         3048 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXHPG010000193 DAXHPG010000000
VERSION     DAXHPG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.193"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            230..2455
                     /locus_tag="ACFM6F_001970"
     CDS             230..2455
                     /locus_tag="ACFM6F_001970"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC6165323.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAEGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRILAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     TKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSATFNQALNA
                     L"
     gene            2598..2906
                     /locus_tag="ACFM6F_001971"
     CDS             2598..2906
                     /locus_tag="ACFM6F_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165324.1"
                     /translation="MKTVPTILISILLATALPASAHGIHKSKPLTMDELPPICQQYFK
                     RAETCYNKAGNKANFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRISNSNAV
                     "
     gene            2896..3048
                     /locus_tag="ACFM6F_001972"
     CDS             2896..3048
                     /locus_tag="ACFM6F_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165325.1"
                     /translation="MPSETMISDGILTSKRTSLIKPAFPASLTRTGLNEVTALTIMAS
                     SHSQKL"
BASE COUNT          856 a          917 c          686 g          589 t
ORIGIN      
        1 tataaaatca atttcaccga aaatatgccg ccgcacatca ggcacagcac ctccgaccgc
       61 caccaaacgc gggaaaacca tttctccctt cctttcacgc cgccgcgttc aaacacgcca
      121 agatcgaaca aagcgcgaac aaagccgcca aaatatgcca taatagccgc gcacattccc
      181 cgcgcaatgt gttccaaaag aaaaaaccaa cctacaagag aataatccta tgactcaaaa
      241 atccaccatt gtttataccc ataccgacga agcccccgcg ctggcgaccc aatcgctgct
      301 gccgattgtg caggcgtttg cccgccacgc cgatattgat gtcaaaactg ccgacatttc
      361 cctgtccggc cgtattttgg cggcattccc cgaatatctg accgaagcgc agcgcgtacc
      421 cgacgcgctt gccgaattgg gcgaactggt gaaacagccc gatgcaaacg taatcaaact
      481 gccgaacatc agcgcgtccg tacctcaact gaccgccgcg attaaagaat tgcagtctaa
      541 aggctttgcc gttcccgatt atcctgccga ccctcaaacc gatgaagaaa aagccgtacg
      601 cgaacgctac gaccgcatca aaggcagcgc ggtaaaccct gtcctgcgtg aaggcaactc
      661 cgaccgtcgc gcccctaaag cggtgaaaaa ctttgcgaaa aaaaatccgc acagtatggg
      721 cgcgtgggcg aaagactcca aaacccacgt cgccactatg caaagcggcg attttttcca
      781 taacgaacaa tccgttaccg tacccgaagc gacttccgta tccatcgtgt tcaccgacaa
      841 acaaggcaac aaaaaagagc tgcgcgcgcc tgttgccctg aaagccggcg aaatcatcga
      901 cgcaaccgtg atgagcaaaa aagccctgct cgccttcctt gccgaacaag tgaaagacgc
      961 gaaggcaaaa ggcgtattgt tctcactgca tatgaaagcc acgatgatga aagtgtccga
     1021 cccgattatc ttcggacacg ccgtcaaagt gttcttcgcg cctgtatttg aaaaattcgg
     1081 cggcaaactg gctgccgcag gtgtcaacgt caacaacggc ttcggcaacc tgctggccaa
     1141 tctggacaaa ctggatgcgg acacccgcgc cgccgtcgaa gccgaaatcg ccgccgttta
     1201 cgccgccaac cctgatttgg cgatggttga ttccgacaaa ggcatcacca acctgcacgt
     1261 tcccagcgat gtcatcgtcg acgcttctat gcctgcgatg attcgtaatt ccggccgtat
     1321 gtgggacaaa aacggcaaag cacaagacac caaagccgtg attcccgaca gcagctatgc
     1381 cggcgtttac caagcaacca tcgacttctg ccgcgaacac ggcgcatttg acccgacaac
     1441 catgggtact gtgcccaacg tcggactgat ggcgcaagcg gcggaagaat acggctcgca
     1501 caacaaaacc tttgaaatcg aagccgacgg tcaggttcaa gtcattgatg cggaaggaaa
     1561 agtcctgatg cagcacgacg ttgaagcagg cgacatctgg cgtatgtgcc aaaccaaaga
     1621 cgctccggtc aaagactggg tgcaactcgc cgtcaaccgc gcccgcctga gcaacacgcc
     1681 tgccgtgttc tggctcgatg aaaaccgtcc gcacgacaag agcctgctcg ccaaggttaa
     1741 agcctacctt gccgaactgg ataccaatgg cctcgacatc cgcatcctcg ctcctgaaga
     1801 agccgccaag ttcagcttgg gtcgtctgaa aaacggcgaa gacaccatct ccgtaaccgg
     1861 taatgtcttg cgcgactacc tgaccgactt gttcccaatt ttggaactcg gcacaagcgc
     1921 gaaaatgctg tctatcgttc cattgatgaa cggcggcggt atgtttgaaa ccggcgcggg
     1981 cggttctgca ccgaaacatg ttcaacaatt cctcgaagaa aaccatttgc gctgggactc
     2041 gctgggcgaa tttctcgcac tcgccgtatc gtttgaacat ctggcgcaaa aaaccggcaa
     2101 caccaaagcc caagtcctcg ccgacacttt ggatgcagcc accgaaaaac tgctgttgaa
     2161 cgacaaatcg cccaaacgca aagcgggcga actcgacaac cgcggcagcc atttctacct
     2221 caccctctac tgggcgcaag aattggcggc gcaagacaaa gatgccgaac tgaaagccgc
     2281 atttacgcca ttggcagccg ctttgaccgc cgacgaagcg aaaatcgttg ccgagctttc
     2341 cgccgtacaa ggcaaagcgg ccgacatcgg cggctactac gccgccaatc ctgaaaaagc
     2401 cgcacaagtg atgcgtccga gcgcgacctt taatcaggca ctaaacgcct tataagcaca
     2461 aaggtaaaaa tgccgtctga acattcgtcg ttcagacggc attagttatt cctatctgcc
     2521 tgattatgat taaacgccgc acccattgga tataaatcat ataatcttta gtttttaccc
     2581 taggaaaaga caataatatg aaaactgttc caaccatcct gattagcatc cttttggcaa
     2641 ccgcccttcc tgcatccgca cacggtatac ataagagcaa gcccctgact atggacgagc
     2701 tgccgccgat ttgccaacaa tatttcaaac gcgccgaaac ctgttacaac aaagccggca
     2761 acaaggcaaa ttttgcacgc aacaacacta aattcctatt ccaagccctg cctgctgccg
     2821 atttgggaca gcgtaagcaa atgtgccaaa tcgctatgga ttcgtttgca gaaaaaaccc
     2881 gcatcagcaa tagcaatgcc gtctgaaaca atgatttcag acggcattct cacatcaaaa
     2941 cgcactagct tgataaaacc cgccttcccc gcatccctga cccgaacagg attaaacgaa
     3001 gtaacggctt taaccattat ggcaagttcc cattctcaaa aattatag
//
LOCUS       DAXHPG010000194         3031 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXHPG010000194 DAXHPG010000000
VERSION     DAXHPG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.194"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..1599
                     /locus_tag="ACFM6F_001973"
     CDS             <1..1599
                     /locus_tag="ACFM6F_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6165326.1"
                     /translation="LAENQSRYDTWKEGGIGRSILHGAAGGLTTGSLGGILAGGGTSL
                     AAPYLDKAAENLGTAGKAAVNALGGAAIGYAAGGNVGTAAVGANVDWNNRQLHNSEIR
                     KIKNNAAKFAKLHPGISQRQAEERLVAQAMRQVDAAYYAKHADTDTAAEQFLRSIAGS
                     FKADGETFLDFAPMKYYRDSGKFAEHTGYRDLRAKLGQNPVHTEAQRNRTIEEKTYGT
                     IGRGVGKGIGNTLANNLQAGPPHYVLGITNYSNWGKELIPYENPVERAIGKQAEDMTE
                     KAVAAVSLGRSLPSKVVNVPSKLAKSIKTAETHKPLVQKRTETYFRVEGGGSGNNQAA
                     KAAKNTEKMKAAIDQTHSGVDLPYDSRQIRAKLEKRYGAENIVSTTIPKKPVQSINNQ
                     TKPDYSSIVYTNKSGKVVNVKYKDPISGESKAVNIAYDNRGLPIFDNVSNFTTKIDNS
                     LSYTGQMRAATKDLWKAIESGQVPKNQFTNEQLTKIKAGNEKIPGYTWHHNAQSSPNN
                     MQLIPNSIHTAVRHTGQKSLKKGQ"
     gene            1602..2078
                     /locus_tag="ACFM6F_001974"
     CDS             1602..2078
                     /locus_tag="ACFM6F_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6165327.1"
                     /translation="MRKLSISSDEGLVPIRTIQQFAEDVGVVFPASYVSLLSKHDHLY
                     PKENIFNFVNQYGENDERDISFLGYKQDLGYEDIYSYSCIDDEYGYAKKVIAFGISAN
                     GDYICFDYRKCNENPCIILMYHDDFYEDENGDTKMVTSHIADSFDAFLDMLYELSD"
     gene            2192..>3031
                     /locus_tag="ACFM6F_001975"
     CDS             2192..>3031
                     /locus_tag="ACFM6F_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6165328.1"
                     /translation="MRFSRFEPKGRLKQQIRFQTTFLSTSHHGNQTKAAHRHIHVGST
                     AGTTTIRSGGDTTLKGAQLIGKGIQADTRNLHIESVQDTETYQSKQQNASAQVTVGYG
                     FSASGDYSQSKIRADHASVTEQSGIYAGEDGYQIKVGNHTDLKGGIITSSQSAEDKGK
                     NRFQTATLTASDIQNHSQYKGESFGLGASASISGKTLGQGAQNKPQNKHLTSVADKNG
                     ASSSVGYGSDSDSQSSITKSGINTRNIHITDEAGQLARTGRTAKETEARIYTGIDTET
                     ADQH"
BASE COUNT         1022 a          661 c          725 g          623 t
ORIGIN      
        1 cttgccgaaa accaaagccg ctacgacacc tggaaagaag gcggcatagg caggagcata
       61 ctgcacgggg cggcaggcgg actgacgacc ggaagcctcg gcggcatact ggccggcggc
      121 ggcacttccc ttgccgcacc gtatttggac aaagcggcgg aaaacctcgg tacggcgggc
      181 aaagcggcgg tcaacgcact gggcggggcg gcaatcggct atgcggcggg cgggaatgtc
      241 ggcacggcgg cagtaggggc gaatgtcgat tggaacaata ggcagctgca caacagcgag
      301 atcagaaaaa ttaaaaataa tgccgctaaa tttgccaaac tccatcccgg catttcccaa
      361 cggcaggcag aagagcggct ggtggcacaa gctatgcgtc aggtcgatgc ggcttattat
      421 gccaaacatg ccgacaccga cacggcggcg gaacaattcc tgcgcagcat agcgggaagt
      481 ttcaaagccg acggggaaac gttcctggat tttgccccga tgaagtatta cagggacagc
      541 ggtaaatttg ccgaacatac aggctatcga gatctaagag caaagttggg acagaatccg
      601 gtccataccg aagcgcaaag gaacagaact attgaagaaa agacctacgg cacaataggc
      661 agaggtgtag gtaaaggcat cggcaatacc ctagccaaca acctgcaggc gggtccgcca
      721 cactatgtac tcggcattac gaactattcg aactggggca aagaacttat tccatacgag
      781 aaccctgttg agcgcgccat cggcaaacag gcagaggata tgacggaaaa agcggtagca
      841 gctgtgtctt tgggccggag tttgccgtca aaggttgtta atgtaccctc taaattagct
      901 aaaagcatta agacagcgga aacacataaa ccactggtac aaaagcgtac ggaaacttat
      961 tttagagttg aaggaggagg aagtggaaat aatcaggcag ccaaagccgc taaaaatacg
     1021 gaaaaaatga aagccgcaat agatcaaacc cacagtggag ttgacctgcc ttatgattcg
     1081 agacaaattc gagctaaact ggaaaaaaga tatggagctg agaatattgt atccactacg
     1141 attccaaaga aaccggtcca aagtatcaat aatcaaacta aaccggacta ctcatctatt
     1201 gtatatacta ataaaagtgg aaaggtagtt aatgttaaat ataaagatcc tatatcaggg
     1261 gaaagtaagg ctgttaatat cgcctatgat aataggggat tacctatatt tgataacgta
     1321 tcaaatttta ccaccaaaat agataattca ttgtcgtata cgggacagat gagggcagct
     1381 actaaagatt tatggaaagc tattgaaagt ggacaagtgc ccaaaaatca atttactaat
     1441 gaacaactaa cgaagattaa agcaggaaat gaaaaaattc ctggttatac atggcatcat
     1501 aatgcgcaaa gctctcctaa taatatgcag ttaattccta atagtattca tacagctgtt
     1561 aggcatactg gtcaaaaatc gttaaagaaa ggtcagtaaa aatgagaaaa ctatcaatat
     1621 catcagatga gggattggtt ccaataagaa caattcaaca atttgctgaa gatgtagggg
     1681 tcgtttttcc cgcgtcatat gtatcacttc ttagtaaaca tgatcattta taccctaaag
     1741 aaaatatttt caattttgtc aatcaatacg gtgaaaatga tgaaagggac atttcatttt
     1801 tagggtataa gcaagattta ggctatgaag atatttatag ctattcatgt attgatgatg
     1861 aatatgggta tgcaaaaaaa gtgattgctt ttgggatttc agccaatggc gattatatct
     1921 gttttgatta cagaaaatgc aatgaaaatc catgtatcat tctgatgtat catgatgatt
     1981 tctatgaaga tgagaatggg gatacgaaaa tggtaactag ccatattgca gatagttttg
     2041 atgccttttt ggatatgcta tatgaacttt cagattaagt gtgtaggttg agttgaaaac
     2101 ccaactaaat taaaaaggtc gtctgaaatc agcaagccgt agcccatatg aaacctaaac
     2161 tcaacaagta ggatgtgtgc ggaacgcacg catgcggttc tcaaggtttg agcccaaagg
     2221 tcgtctgaaa caacaaatac ggtttcagac gacctttctt tcaacaagcc accacggcaa
     2281 tcagacaaaa gcagcacatc gccacatcca tgtcggcagt acggccggca caaccaccat
     2341 ccgcagcggc ggggatacca ccctcaaagg tgcgcagctc atcggcaaag gcatacaggc
     2401 agatacgcgc aacctgcata tagaaagtgt tcaagatact gaaacctatc aaagcaaaca
     2461 acaaaacgcc agtgcacaag tgaccgtagg ttatggcttc agtgccagtg gcgattacag
     2521 ccaaagcaaa atccgagccg accatgcttc ggtaaccgag caaagcggta tttatgccgg
     2581 agaagacggc tatcaaatca aggtcggaaa ccatacagac ctcaaaggcg gcatcatcac
     2641 cagcagccaa agcgcagaag acaagggtaa aaaccgcttt cagacggcca cccttactgc
     2701 cagcgacatt caaaaccaca gccaatacaa aggcgaaagt tttggattgg gcgcaagtgc
     2761 gtccataagc ggcaaaacac tgggacaggg cgcacaaaat aagcctcaaa acaaacacct
     2821 gacaagcgtg gccgataaaa acggcgcaag ttcatcagtg ggttatggca gcgacagcga
     2881 cagtcaaagc agcatcacga aaagcggcat caacacccgc aacatacaca tcaccgacga
     2941 agcgggacaa cttgcccgaa caggcaggac tgccaaagaa accgaagcgc gtatctacac
     3001 cggcatcgac accgaaactg cggatcaaca c
//
LOCUS       DAXHPG010000195         3025 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXHPG010000195 DAXHPG010000000
VERSION     DAXHPG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.195"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>3025
                     /gene="pilC"
                     /locus_tag="ACFM6F_001976"
     CDS             <1..>3025
                     /gene="pilC"
                     /locus_tag="ACFM6F_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6165329.1"
                     /translation="SAQTYSYVIVMNGQKLPEVKNGNQSSNQDKRRQATFTARFIGAK
                     RNTTFSFDNTDELVAEKKDVVVFGAATYLPPYGKVSGFDEKMLKTRLDALDWIRTTRP
                     GLVGYSYQGSTCNSGDCPDVSYKTQFTFGNQGLKKKATGKLDIDADKSRDNSPIYKLS
                     DYSWLGVSFNLSSESIVESKKLKKVESSFNEEVTQNNGTFSQYKDKNLIYTTGDVRNR
                     GNQAHRDKPHAVAFYLNAKLHLLDKKQIKDIMRGSELNLGDLKTRIEPTDAWKNKRHR
                     TLTVGQWEFKDTGKISVKLKLPEVKAGRCINKPNPNPKSKAPSPALTAPALWFVPVQN
                     GKAQMYSASVSTYPDSSSSRIFLQNLKRKNDPNKPGRHSLETLTGNDIQSREPTFNQR
                     QTVIRLDSGVQQIKLQDNEVANFNGNDGENDTFGIVSEGSFMPDTSEWKKVLLPWTVR
                     ASNDDNQFKTFNQEEKDNKPKYSQKYRSRDNSKHERDLGDIVNSPIVAVGGYLATSAN
                     DGMVHIFKKNGGGDDRNYSLKLSYIPGTMPRKDIQSQDSTLAKELRAFAEKGYVGDRY
                     GVDGGFVLRQVELSGQKHVFMFGAMGFGGRGAYALDLTKADGNDPTKASLFDVKDNGN
                     NGNNGNNSVQLGYTVGTPQIGKTHNGKYAAFLASGYATKTIDDQQNKTALYVYDLESS
                     GTLIKKIDVPGGKGGLSSPTLVDKDLDGTVDIAYAGDRGGNMYRFDLKDWSVRTIFSG
                     NKPITSAPAISQLKDKRVVIFGTGSDLSEDDVDNTDEQHIYGIFDDDTATTGSVNFSG
                     TGGGLLEQHLTQENKTLFLTDYKRSDGSGSKGWMVKLQPGQRVTVKPTVVLRTAFVTI
                     HKYTGTDKCGAETAILGINTADGGKLTKKSARPIVPAANSKVAQYSGDKKTSSGKSIP
                     IGCMEKDNGIVCPNGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPDGKRAGKN
                     NRCFSQKGVRTLLMNDLDSLDITGPTCGMKRISWRE"
BASE COUNT          886 a          754 c          765 g          620 t
ORIGIN      
        1 gtcggcgcag acgtattcat acgttattgt aatgaacgga caaaagctac ctgaagtaaa
       61 gaatgggaac caatcgtcga accaagacaa gcgccgccaa gctaccttta cggcaaggtt
      121 tattggagca aaaagaaaca ctactttctc attcgataat actgatgagc ttgttgctga
      181 aaaaaaagat gttgtcgttt tcggcgcagc cacctacctg ccgccctacg gcaaggtttc
      241 tggttttgat gaaaaaatgc tgaaaacgcg cctcgatgcc cttgattgga ttcgtacgac
      301 ccgcccgggg ttggtaggct acagctacca aggtagcaca tgcaacagcg gagactgtcc
      361 tgatgttagc tataaaaccc aatttacctt cggtaatcaa gggttgaaaa aaaaagcaac
      421 cggcaagctg gatatagacg cagacaaaag ccgcgacaat tcgcccattt acaaattgtc
      481 ggattattct tggttgggcg tgtctttcaa tttgagcagc gagagcatcg tcgaatcaaa
      541 gaaactcaag aaagtggaat cttcttttaa cgaggaggtg acgcagaata atggtacgtt
      601 cagccaatac aaagacaaaa acctcattta tacgacaggc gatgtccgta ataggggtaa
      661 tcaagcccat cgggacaaac cccacgccgt cgccttttat ctgaacgcca aactgcacct
      721 gctggataaa aaacaaatca aagatatcat gcgaggcagt gaacttaatt tgggtgattt
      781 gaaaacgcgt atcgagccga cggacgcgtg gaagaataag aggcatcgca ctttaactgt
      841 tggtcagtgg gagttcaaag ataccggaaa aatcagcgtc aaactcaaat tgccggaagt
      901 caaagcaggc cgctgcatca ataaaccgaa ccccaatccc aaatccaaag ccccttcgcc
      961 ggccctgacc gcccccgcgc tgtggttcgt acctgtgcaa aatggtaagg cgcagatgta
     1021 ttccgcttcg gtttctacct accccgacag ttcgagcagc cgcatcttcc ttcaaaatct
     1081 gaaaagaaaa aacgacccca acaaacccgg ccgccattcc ctcgaaacct tgactgggaa
     1141 tgatattcaa agtcgagagc cgactttcaa ccagcggcaa accgtcatcc gattggatag
     1201 cggcgtacag cagatcaaac ttcaagacaa tgaggtcgcc aattttaatg gaaatgacgg
     1261 cgaaaacgac actttcggca ttgttagtga agggagcttc atgcctgata ccagcgagtg
     1321 gaaaaaagta ttgctgcctt ggacggttcg tgcttccaat gatgacaatc aatttaaaac
     1381 atttaaccaa gaagaaaaag acaacaagcc aaaatacagt caaaaatacc gcagccgcga
     1441 taacagcaag cacgagcgcg atttgggcga catcgtcaac agccccatcg tggcggtcgg
     1501 cgggtatttg gctacttccg ccaacgacgg gatggtgcat atcttcaaaa aaaacggcgg
     1561 cggggatgac cgcaactata gtctgaagct cagctacatc cccggcacga tgccgcgcaa
     1621 ggatattcaa agccaagact ccacccttgc caaagagctg cgcgcctttg ccgaaaaagg
     1681 ctatgtgggc gaccgctacg gcgtggacgg cggctttgtc ttgcgccaag tcgaactgag
     1741 cgggcaaaaa cacgtgttta tgttcggcgc gatgggcttt ggcggcagag gtgcgtatgc
     1801 cttggatttg accaaagccg acggcaatga cccgacaaaa gcctctttgt ttgatgtaaa
     1861 agataacggc aataatggca ataacggcaa taatagcgtg caattaggct acaccgtcgg
     1921 cacgccgcaa atcggcaaaa cccacaacgg caaatacgcc gctttcctcg cctccggtta
     1981 tgcgactaaa accattgacg accaacaaaa taaaaccgcg ctgtatgtgt atgatttgga
     2041 aagcagcggt acgctgatta aaaaaatcga tgtacccggc ggcaagggcg ggctttcgtc
     2101 ccccacgctg gtggataaag atttggacgg cacggtcgat atcgcctatg ccggcgatcg
     2161 cggcgggaat atgtaccgct ttgatttgaa agactggtct gtacgcacca ttttcagcgg
     2221 caacaaaccg attacttccg cgcccgccat ttcccaactg aaagacaaac gcgtggttat
     2281 cttcggcacg ggcagcgatt tgagtgagga tgatgttgat aatacggatg aacaacatat
     2341 ttacggtatc ttcgacgacg atacggcgac gacgggttct gtaaacttca gcggtacggg
     2401 aggcgggctg ctcgagcaac accttactca ggaaaataaa accttattcc tgaccgatta
     2461 caagcgatcc gacggatcgg gcagtaaggg gtggatggtg aaattgcagc ccggacaacg
     2521 cgttaccgtc aaaccgaccg tggtattgcg taccgccttt gtaaccatcc ataaatatac
     2581 gggtacggac aaatgcggcg cggaaaccgc cattttgggt atcaataccg ccgacggcgg
     2641 caagctgacc aagaaaagcg cgcgcccgat tgtgccggca gccaattcga aggtcgcgca
     2701 atattccggc gataagaaaa cttccagcgg caaatccatc cctataggtt gtatggaaaa
     2761 agacaatgga atcgtctgcc cgaacggata tgtttacgac aaaccggtta atgtgcgtta
     2821 tctggatgaa aagaaaacag acggattttc aacaacggca gacggtgatg cgggcggcag
     2881 cggtatagac cccgacggca agcgtgccgg caaaaacaac cgctgcttct cccaaaaagg
     2941 ggtgcgcacc ctgctgatga acgatttgga cagcttggac attaccggcc cgacgtgcgg
     3001 tatgaaacga atcagctggc gtgaa
//
LOCUS       DAXHPG010000196         2987 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXHPG010000196 DAXHPG010000000
VERSION     DAXHPG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.196"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>2987
                     /gene="pilC"
                     /locus_tag="ACFM6F_001977"
     CDS             <1..>2987
                     /gene="pilC"
                     /locus_tag="ACFM6F_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6165330.1"
                     /translation="AQTHKYAIIMNERKQPEVKSNVPSSIKDKDREREYTHHPSRGGG
                     GSVSFNNTDTLVSQQSGTAVFGTATYLPPYGKVSGFDTAKLTERNNAVNWIHTTHPGL
                     IGYSYAGVVCRDSTGCPKLVYKTRFSFDNPDLAKTGGRLDRHTEPSRDNSPIYKLKDH
                     PWLGVSFNLGSDNTVKNGKSSSKLISSFNENNNNQTIVSTTEGDPISLGDQQREHTAV
                     VYYLNAKLHLLDKKQIQNITDKTVQLGVLKPSIDVKTQRTGIAGILAFHAQWDIKDTG
                     QIPVKLGLQQVKAGRCINKPNPNPNKKDLSPALTAPALWFGPVQNGKVQMYSASVSTY
                     PDSSSSRIFLQNLKRKTDPGRPGRYSLATLNKSDIESREPTFTGRQTIIRLDGGVQQI
                     KLDKSNEATGLNGNTNNNTFGIVKEYSVNPETNEWKKVLLPWTVRSFSNDNEFVKFNK
                     ESDKYSQRYRIRENGNNSKRDLGDIVNSPIVAVGGYLATSANDGMVHIFKKGNGGDDR
                     NYSLKLSYIPGTMPRKDIESKDSNLAKELRTFAEKSYVGDRYGVDGGFVLRKVDNLNG
                     QNRVFMFGAMGFGGRGAYALDLTKADGSDPTKASLFDVKDNGNNGNNRVELGYTVGTP
                     QIGKTHDGKYAAFLASGYATKEINSTDNKTALYVYDLESSGTLIKKIDVPGGKGGLSS
                     PTLVDKDLDGIVDIAYAGDRGGKMYRFDLSNQDSSQWTVRTIFEGTKPITSAPAISQL
                     KDKRVVIFGTGSDLSEEDVDNNDIQSIYGIFDNDTDTGFAQDGLGKGLLEQKLEKDKD
                     GKTLFLSDYKRSDGSGDKGWVVKLEAGQRVTVKPTVVLRTAFVTIHKYTGNDKCGAET
                     AILGINTADGGKLTKKSARPIVPEANTAVAQYSGHKKTSSGKSIPIGCMEKGNETVCP
                     NGYVYGKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLL
                     MNDLDSLDITGPTCGMKRISWRE"
BASE COUNT          871 a          755 c          750 g          611 t
ORIGIN      
        1 tggcgcaaac ccataaatac gctattatca tgaacgagcg aaagcagccc gaggtaaagt
       61 cgaatgtgcc atcttcaata aaggacaaag acagggagcg cgaatatact catcatccgt
      121 ccagaggagg aggaggctct gtctcattca acaataccga tacccttgtt tcccaacaaa
      181 gcggtactgc cgtttttggc acagccacct acctgccgcc ctacggcaag gtttccggtt
      241 ttgataccgc taagctgacc gagcgcaaca atgccgttaa ttggattcat acgacccacc
      301 cagggttgat aggctacagc tacgccggtg tcgtatgcag agacagcaca ggctgtccca
      361 aacttgtcta taaaacccga ttttccttcg ataatcccga cttggcaaaa acaggaggca
      421 ggctggatag gcacacagag ccaagccgcg acaattcgcc catttacaaa ttgaaggatc
      481 atccatggtt gggcgtgtct ttcaatttgg gcagcgacaa taccgtcaaa aatggcaaat
      541 catccagcaa attgatatct tcttttaatg aaaacaataa taatcaaacc atcgtctcta
      601 cgacagaagg tgaccctatt tcccttggcg accagcagcg cgaacatacc gccgtggtct
      661 attatctgaa cgccaaactg cacctgctgg acaaaaaaca gattcaaaat atcaccgaca
      721 aaacagtgca gttgggtgtc ttgaagccga gcatcgatgt gaagacacaa aggacgggga
      781 ttgccggcat tctagctttt catgctcagt gggacattaa agataccggg cagattccag
      841 tcaagctcgg cctgcagcaa gtcaaagcag gccgctgcat caataaaccg aaccccaatc
      901 ccaacaaaaa agacctttcg ccggcactga ctgcccccgc gctgtggttc ggacctgtgc
      961 aaaatggtaa ggtgcagatg tattccgctt cggtttctac ctaccccgac agttcgagca
     1021 gccgcatctt ccttcaaaat ctgaaaagaa aaaccgaccc cggcagaccc ggccgctatt
     1081 ccctcgcaac cttgaataag tcggatattg aaagtcgaga gccgactttc acagggcggc
     1141 aaacaatcat ccgattggat ggcggcgtac agcagatcaa actggataaa agcaatgagg
     1201 ccaccggttt aaatggaaat accaacaaca acactttcgg cattgttaag gaatatagcg
     1261 tcaatcctga gaccaatgag tggaaaaaag tgctgctgcc ttggacggtt cggagtttta
     1321 gtaatgacaa tgaatttgta aaattcaaca aagaatcaga caaatacagc caaagatacc
     1381 gcatccgcga aaacggcaac aacagcaagc gcgatttggg cgacatcgtc aacagcccca
     1441 tcgtggcggt cggcgggtat ttggctactt ccgccaacga cgggatggtg catatcttca
     1501 aaaaaggcaa cggcggggat gaccgcaact atagtctgaa gctcagctac atccccggca
     1561 cgatgccgcg caaggatatt gaaagcaaag actccaacct cgccaaagag ctgcgcacct
     1621 ttgccgaaaa aagctatgtg ggcgaccgct acggcgtgga cggcggcttt gttttgcgca
     1681 aagttgataa cttaaacggg caaaaccgcg tgtttatgtt cggcgcgatg ggctttggcg
     1741 gcagaggcgc atacgccttg gatttgacca aagccgacgg cagtgacccg acaaaagcct
     1801 ctttgtttga tgtaaaagat aacggcaata atggcaataa tcgcgtggaa ttaggctaca
     1861 ccgtcggcac gccgcaaatc ggcaaaaccc acgacggcaa atacgccgct ttcctcgcct
     1921 ccggttatgc gactaaagaa attaacagca cagacaataa aaccgcgctg tatgtgtatg
     1981 atttggaaag cagcggcacg ctgattaaaa aaatcgatgt acccggcggc aagggcgggc
     2041 tttcgtcccc cacgctggtg gataaagatt tggacggcat tgtcgatatc gcctatgccg
     2101 gcgatcgcgg cggcaagatg taccgctttg atttaagcaa tcaagattct agtcaatgga
     2161 ctgtacgcac tatttttgaa ggcacaaaac cgattacttc cgcgcccgcc atttcccaac
     2221 tgaaagacaa acgcgtggtt atcttcggca cgggcagtga tttgagtgag gaggatgttg
     2281 ataataatga tatacaatct atttacggta tttttgacaa tgacacagac acgggttttg
     2341 cgcaagacgg gctgggcaaa gggctgctcg agcaaaagct ggaaaaagac aaagacggca
     2401 aaacgctgtt tttaagcgac tacaaaaggt ctgacggctc gggcgacaag ggctgggtag
     2461 tgaaattgga agccggacag cgcgttaccg tcaaaccgac cgtggtattg cgtaccgcct
     2521 ttgtaaccat ccataaatat acgggtaatg acaaatgcgg cgcggaaacc gccattttgg
     2581 gcatcaatac tgccgacggc ggcaagctga ccaagaaaag cgcgcgcccg attgtgccgg
     2641 aagccaatac ggctgtcgcg caatattccg gccataagaa aacttccagc ggcaaatcca
     2701 tccctatagg ttgtatggaa aaaggcaatg aaaccgtctg cccgaacgga tatgtttacg
     2761 gcaaaccggt taatgtgcgt tatctggatg aaaagaaaac agacggattt tcaacaacgg
     2821 cagacggcga tgcgggcggc agcggtatag accccgccgg caagcgttcc ggcaaaaaca
     2881 accgctgctt ctcccaaaaa ggggtgcgca ccctgctgat gaacgatttg gacagcttgg
     2941 acattaccgg cccgacgtgc ggtatgaaac gaatcagctg gcgtgaa
//
LOCUS       DAXHPG010000197         2978 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXHPG010000197 DAXHPG010000000
VERSION     DAXHPG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.197"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            112..2877
                     /gene="tdfH"
                     /locus_tag="ACFM6F_001978"
     CDS             112..2877
                     /gene="tdfH"
                     /locus_tag="ACFM6F_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC6165331.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQDGQLKFNSDSGKWER
                     DLQRQQWKYPWYKKYENPQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYANLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYREHCNQSCGIYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     ITMSYKF"
BASE COUNT          846 a          749 c          726 g          657 t
ORIGIN      
        1 caattgaaat cttcagacgg tatatcgaat ttacactttt taatgtttat gccgcctaaa
       61 aaaatgctaa tatatttctt aattgtctga ctgtttattg ttgaggaaaa tatgagatct
      121 tctttccggt tgaagccgat ttgtttttat cttatgggtg ttatgctata tcatcatagt
      181 tatgccgaag atgcagggcg cgcgggcagc gaggcgcaga tacaggtttt ggaagatgtg
      241 cacgtcaagg cgaagcgcgt accgaaagac aaaaaagtgt ttaccgatgc gcgtgccgta
      301 tcgacccgtc aggatatatt caaatccagc gaaaacctcg acaacatcgt acgcagcata
      361 cccggtgcgt ttacacagca agataaaagc tcgggcattg tgtctttgaa tattcgcggc
      421 gacagcgggt tcgggcgggt caatacgatg gtggacggca tcacgcagac cttttattcg
      481 acttctaccg atgcgggcag ggcaggcggt tcatctcaat tcggtgcatc tgtcgacagc
      541 aattttattg ccggactgga tgtcgtcaaa ggcagcttca gcggctcggc aggcatcaac
      601 agccttgccg gttcggcgaa tctgcggact ttaggcgtgg atgacgtcgt tcagggcaat
      661 aatacctacg gcctgctgct aaaaggtctg accggcacca attcaaccaa aggtaatgcg
      721 atggcggcga taggtgcgcg caaatggctg gaaagcggag catctgtcgg tgtgctttac
      781 gggcacagca ggcgcagcgt ggcgcaaaat taccgcgtgg gcggcggcgg gcagcacatc
      841 ggaaattttg gcgcggaata tctggaacgg cgcaagcaac gatattttgt acaagacggc
      901 cagttgaaat tcaattccga cagcggaaaa tgggagcggg acttgcaaag acaacagtgg
      961 aaatacccgt ggtataaaaa atacgaaaac ccccaagaac tgcaaaaata catcgaagag
     1021 catgacaaaa gctggcggga aaacctggcg ccgcaatacg acatcacccc catcgatccg
     1081 tccagcctga agcagcagtc ggcaggtaat ctgtttaaat tggaatacga cggcgtattc
     1141 aataaataca cggcgcaatt tcgcgattta aacaccaaaa tcggcagccg caaaatcatc
     1201 aaccgcaatt atcaattcaa ttacggttta tctttaaact catatgccaa cctcaatctg
     1261 accgcagcct acaattcggg caggcagaaa tatccgaaag ggtcgaagtt tacaggctgg
     1321 gggcttttaa aagattttga aacctacaac aacgcgaaaa tcctcgacct caacaacacc
     1381 gccaccttcc ggctgccccg cgaaaccgag ttgcaaacca ctttgggctt caattatttc
     1441 cacaacgaat acggcaaaaa ccgctttcct gaagaattgg ggctgttttt cgacggtccg
     1501 gatcaggaca acgggcttta ttcctatttg gggcggttta agggcgataa agggctgctg
     1561 ccccaaaaat caaccatcgt ccaaccggcc ggcagccaat atttcaacac gttctacttc
     1621 gatgccgcgc tcaaaaaaga catttaccgc ttaaactaca gcaccaatac cgtcggctac
     1681 cgtttcggcg gcgaatatac gggctattac ggctcggatg acgaatttaa gcgggcattc
     1741 ggagaaaact cgccgacata cagggaacat tgcaaccaga gctgcggaat ttatgaaccc
     1801 gtattgaaaa aatacggcaa aaagcgcgcc aacaaccatt cggtcagcat tagtgcggac
     1861 ttcggcgatt atttcatgcc gttcgccagc tattcgcgca cacaccgtat gcccaacatc
     1921 caagaaatgt atttttccca aatcggcgac tccggcgttc acaccgcctt aaaaccagag
     1981 cgcgcaaaca cttggcaatt tggcttcaat acctataaaa aaggattgtt aaaacaagat
     2041 gatacattag gattaaaact ggtcggctac cgcagccgca tcgacaacta catccacaac
     2101 gtttacggga aatggtggga tttgaacggg aatattccga gctgggtcag cagcaccggg
     2161 cttgcctaca ccatccaaca ccgcaatttc aaagacaaag tacacaaaca cggttttgag
     2221 ttggagctga attacgatta tgggcgtttt ttcaccaacc tttcttacgc ctatcaaaaa
     2281 agcacgcaac cgaccaactt cagcgatgcg agcgaatcgc ccaacaatgc gtccaaagaa
     2341 gaccaactca aacaaggtta tgggttgagc agggtttccg ccctgccgcg agattacgga
     2401 cgtttggaag tcggtacgcg ctggttgggc aacaaactga ctttgggcgg cgcgatgcgc
     2461 tatttcggca agagcatccg cgcgacggct gaagaacgct atatcgacgg caccaacggg
     2521 ggaaatacca gcaatgtccg gcaactgggc aagcgttcca tcaaacaaac cgaaaccctt
     2581 gcccgccagc ctttgatttt tgatttttac gccgcttacg agccgaagaa aaaccttatt
     2641 ttccgcgccg aagtcaaaaa tctgttcgac aggcgttata tcgatccgct cgatgcgggc
     2701 aatgatgcgg caacgcagcg ttattacagc tcgttcgacc cgaaagacaa ggacgaagac
     2761 gtaacgtgta atgctgatgg cttgtgcaac ggcaaatacg gcggcacaag caaaagcgta
     2821 ttgaccaatt ttgcacgcgg acgcaccttt ttgataacga tgagctacaa gttttaaagg
     2881 cagcccgcgt tttgtggaaa accgcaatgc cgtctgaaag cccttcagac ggcatttgtt
     2941 tccccaaacg catcatcctg ccgcaagcct atgccaat
//
LOCUS       DAXHPG010000198         2883 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXHPG010000198 DAXHPG010000000
VERSION     DAXHPG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.198"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(97..573)
                     /gene="greA"
                     /locus_tag="ACFM6F_001979"
     CDS             complement(97..573)
                     /gene="greA"
                     /locus_tag="ACFM6F_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC6165332.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            968..2269
                     /gene="aroA"
                     /locus_tag="ACFM6F_001980"
     CDS             968..2269
                     /gene="aroA"
                     /locus_tag="ACFM6F_001980"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC6165333.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGKRQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     ENYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPKTLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETTE"
     gene            complement(2312..2845)
                     /locus_tag="ACFM6F_001981"
     CDS             complement(2312..2845)
                     /locus_tag="ACFM6F_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6165334.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          606 a          791 c          775 g          711 t
ORIGIN      
        1 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataatt
       61 tcctgcgtgt gtcgggtgta tcgaaatcaa gccgaatcaa atatatcgga cttcgataat
      121 gtcgtattcg cgcacgccgc ccggggcttg gacttccgcc gtatccccct cttccttgcc
      181 gattaaggcg cgggcgatgg gtgagccgac atagattttg ccctgtttga tgtcggcttc
      241 gtcttcgccg acaatttggt aggtaacgtg ttcttccgtt tccaaatctt ccagcgtaac
      301 cgtcgtaccg aacacgattt tgccttcggc gtggatttcg gtcggattga tgatgtgggc
      361 aacggaaagt ttgtgttcca actcggaaat gcggccctcg ataaaacctt ggcgttcttt
      421 ggcggcttca tattcggcgt tttcggacaa atcgccgtgc gatctggctt cagcaatcgc
      481 ttcgatcact tcggggcgcg ccacgctttt aagctgctgc aattcctgtt tcagcaattc
      541 cgcaccgcgt acggtcaggg ggattttttg catcggtctt ttttctccat attccggcac
      601 accggtttgc ggcagcaaac ataccgcata ccgtcttgtt ttgtgcgtcc ggatattaaa
      661 ataaaaacac aagccgcccg gaaaatcggc ggcttgtctg tcgttgaaca gcagctattc
      721 taccaaattc tatgaaattg gcaatcgtgc cgtgccgtcc gcaaacacgc tatgtccgca
      781 acaaaagccg aaaatatgcc ggcaaaaaaa ttttagaaac aaaaaattta aaaataatca
      841 attttcggca taaaaaacca catttacgga ctttaaaacc gaaaatgcca aacctgagat
      901 ttttcataca gcatttgcac cagtataatg caggctgttt ttatctttat aatattgacg
      961 ttttgccatg accgaatccg tccgcctccc cgtcgcccgt ctcaaacctt ccaccgtcgc
     1021 cctgcccggc tccaaaagca tcagcaaccg caccctgctg cttgccgcct tgtccgacaa
     1081 tgcttgcgaa atccattccc tgctcaaatc cgacgatacc gaccgtatgc tcgaagcact
     1141 cgataaactc ggcgttcaaa tcgaatatct tgccgaagac cgtctgaaag tgcacggcac
     1201 aggcggacgc ttccccaacc gcactgccga tttgtttttg ggcaacgcgg gcacggcgtt
     1261 ccgtccgctg actgccgcac ttgccgtttt gggcggcgat tatcatctgc acggcgtgcc
     1321 tcgtatgcac gaacgcccca tcggcgattt ggccgatgcg ctgcggattg ccggcgccga
     1381 tgtcgaatat ctcggcaagg aacactatcc gccgcttcat atcggcaaac gtcaagacaa
     1441 cggcgaacgc gtgattccga ttaaaggcaa tgtgtccagc cagtttctga ccgccctttt
     1501 aatggcgttg ccgctgaccg ggcaggcgtt tgaaatccgt atggtcggcg aattgatttc
     1561 caagccctat atcgacatta ctttaaaact gatggcgcaa ttcggcgtac aggttgccaa
     1621 tgaaaactac cgcgtcttca aaattcccgc cgatgcgcac taccacgcgc ccgaacactt
     1681 gcacgtcgaa ggcgatgcct ccagcgcgtc ctacttcctc gcagccggtt tgattgctgc
     1741 cacgcccgtg cgcgtgaccg gcatcggcgc aaacagcata cagggcgatg tcgcctttgc
     1801 ccgcgagctg gaaaaaatcg gggcggacgt ggtttggggc gaaaacttcg tcgaagtttc
     1861 acgcccgaag gaacgtgccg tccaatcctt tgatttggat gcgaaccata tccccgatgc
     1921 cgccatgacc ctcgccatcg tcgcgcttgc tacagggcaa acctgcacgc tgcgcaacat
     1981 cggttcgtgg cgcgtcaaag aaaccgaccg catcgccgcg atggcaaacg agttgcgcaa
     2041 actcggggca aaagtcgtcg aagaagccga agcaattcac atcaccccgc ccaaaacgct
     2101 gacacccgac gccgtcatcg acacgtacga cgaccaccgc atggcgatgt gtttctcgct
     2161 ggtttcgctg ttgggcgtac ccgtcgtcat caacgatccg aaatgcaccc ataaaacctt
     2221 cccgacttat ttcgacgtgt tctcttcact gaccgaaaca acggaataag gcggcatttt
     2281 gccgcgattc cggcgcggcg cggcgggcgg ctcattctgt aaagaaagta tgtgcgccga
     2341 ggtagttttt ggcgtaaaac ggtgtggaga gtttttcggt tttgatggtt ttgccgctgc
     2401 tgggggcatg gatgaattcg ccgttgccga tatagagtcc gacgtgtgag tagcggtgtg
     2461 tgccgccggt gttgaagaat acgaggtcgc cggccttaag gcggctgtcg gggattttgc
     2521 ggcttgccgc cgccatgtcg cgggcggtgc gcggcagctt gacgttgagg gcgtttttgt
     2581 aaacgaattg aatcatgccg ctgcaatcga agccggttgc ggtgctgctg ccgccccatt
     2641 tgtagggcgt gccgatgagt ccgaggctgt ggagcatgag ttcctgcgag ccttgtgtgc
     2701 ggtcgatgtg gctgatgcgg acggcttgga tttgccggac tgtctgtttg ggtttcggtt
     2761 ggcggtgttt gccggaggtc gtgccgcatg aggcgaggag cagtgcgctg agacagagga
     2821 aaagggtttt gtcgggggga aacatggttt ttcctttgcg ggttcggata tccgtctgaa
     2881 ggt
//
LOCUS       DAXHPG010000199         2793 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXHPG010000199 DAXHPG010000000
VERSION     DAXHPG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.199"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..236
                     /locus_tag="ACFM6F_001982"
     CDS             <1..236
                     /locus_tag="ACFM6F_001982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165335.1"
                     /translation="HDDRGFSDTEVFYRLIVEILPIFFPIPSGLQMVDDSVCFSAVCR
                     SDQGAAVEGAGVGFLKNLVPNILFQIRGRSPEN"
     gene            complement(274..651)
                     /locus_tag="ACFM6F_001983"
     CDS             complement(274..651)
                     /locus_tag="ACFM6F_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC6165336.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRRKYPYPMDIPRDIVIGIGSIIEFLMVPNWKLFTIKSSPWLPKSIKINERYERFR
                     VMLRYIFTEKDIVNVRFDYYNKK"
     gene            complement(682..1080)
                     /locus_tag="ACFM6F_001984"
     CDS             complement(682..1080)
                     /locus_tag="ACFM6F_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC6165337.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGSIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQHSKGRLR"
     gene            complement(1086..1732)
                     /locus_tag="ACFM6F_001985"
                     /pseudo
     CDS             complement(1086..1732)
                     /locus_tag="ACFM6F_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
     gene            complement(1896..2213)
                     /locus_tag="ACFM6F_001986"
     CDS             complement(1896..2213)
                     /locus_tag="ACFM6F_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165338.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(2216..>2793)
                     /gene="mafB"
                     /locus_tag="ACFM6F_001987"
     CDS             complement(2216..>2793)
                     /gene="mafB"
                     /locus_tag="ACFM6F_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6165339.1"
                     /translation="AAGALNPFISAGEAVDQWMQENPNAAETVEALVNVLPFAKVKNL
                     TKAAKPGKAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQG
                     KHISGHRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGK
                     SGLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
BASE COUNT          738 a          615 c          502 g          938 t
ORIGIN      
        1 aacacgacga tcggggtttc agcgacaccg aggtatttta ccgtctaatc gtggagattt
       61 taccgatttt ctttcctatt ccatccggtc ttcaaatggt tgatgacagt gtttgctttt
      121 cagccgtctg ccgttctgac caaggtgctg ccgtggaagg agcaggtgta ggatttttga
      181 agaacttagt tcccaatatc ctgtttcaaa taaggggaag gtcgcctgaa aattaaacac
      241 ggtttcagac ggcattttga ttgccggggt ttgctatttt ttgttgtaat aatcaaatcg
      301 tacgttgact atgtctttct cggtaaaaat ataacggagc attactctga acctttcata
      361 acgctcatta attttgatac ttttaggcaa ccaaggggaa gatttaattg taaaaagttt
      421 ccaatttgga accattaaaa actcaataat gctaccgatt ccaatcacga tatcccttgg
      481 tatatccatc ggataaggat attttctcct aacctcgatt aaagcattct ccaatttcca
      541 atattcttca tcatcccaca ccccgtcatc ataccatttg ccaataaatg aattttcgtc
      601 atactcctta aaacaaggga tgtttcttct aaaatccttg aactcgcaca taataatgta
      661 tctccaatat aattaaactt ttcatctcaa tctaccttta ctatgttgta ttggaaagta
      721 aaaaaatttc cagtcctcta catctagatc agtaaaaata taacggagca ttactctgaa
      781 cctttcataa cgctcattaa ttttgacact tttaggcaac caaggggaag ctttaatttc
      841 aaaaagtttc caattttgaa ccattaaaaa atcaataatg ctacctattc caatcacgat
      901 gtcccttggt atatccatcg gataaggata ttttctccta acctcgatta aatcattctc
      961 caatttccaa tattcttcat catcccacac cccgtcatca taccatttgc caataaatga
     1021 attttcgtca tactccttaa aacaagggat gtttcttcta aaatccttga actcgcacat
     1081 cataattaat ctccaatacg atttaggttt ttatcaaatg taccgtttct tgtttctttt
     1141 ctgttcagct tcttgggtga agatgcttct ttccaagccc ctccattatg tgaatctaca
     1201 tcgcgtgata tataactctt tcctttttta aaaatagcag catcatttct cgttctttct
     1261 tttatttttc tatatcccaa tttctttgct gctgcatatg cttctgaatc attcccatat
     1321 acgggggtag atggtgtttt tcttggcgga cacgcattat gaacccaaac cccttccgtt
     1381 ccgcctgatt acccttgacg aagtaggtat gccagtcggc aacggtcaga ttgtaggctt
     1441 tgagcggctg ctgtttgagg gtaatgtttt gaaccgtctg ttttgcaccg ctttcggaaa
     1501 gcagggtgtc gccttttttc agacgacctg cctgtatcca ctttccttga ctgtaaaacg
     1561 ggtggatttt attggaaacg agggtctgta ttttaccgat gccgtctgaa atttcaatgt
     1621 aaacggtttc ttgatacgga ttgccgtatc gggcggtaac gggtttgtat cccgtttttc
     1681 cgcttgcctc gtccttggcg aagacgcggt cgccggttcg gatacgggca atggcttttc
     1741 agccgtctgc cgttttgacc aaggtgctgc cgtggaagga gcaagtgtag gatttttgaa
     1801 gaactcagtt ctcaatatcc tatttcaaac gaaggaaatg ccgtctgaaa attaaacacg
     1861 gtttcagaag acattgtgat tgttggtttt ttaacctacg cttgcgaaat taattccttt
     1921 ctcctgaata ctgcaccatt tatttggttt aactccatag aagaatgatt agaataaata
     1981 cattcaaagt cagcttcatc aaaatattct ccacacatag accataactc tgtaatcaaa
     2041 tattccatat cttcatggtc ttctgtgatg gcttctcttg ataaataatt tttgattttt
     2101 aaaaaccttc ttccttcctc gtaatctacg gtaattaacc tgatatctga gtgtatcatg
     2161 cccagcattg ctatattcat accgactcgc agccagcttg gtaatatatt cataactatt
     2221 gcaccttttt aatggtttta gggttagccg gaacaatgtg aactccattt ttacctgaat
     2281 gaatcgtacc aaaattggta cttaatcctg atttcccatc gctgcctata ggatacccaa
     2341 aatcaacaac aggatttcct cttgctcctt tagaaataac tggataagca cctgaatgta
     2401 ttccgttcaa caattcttga ggattaatgt tttgatttaa agtactctta ccttcaatgt
     2461 agtttctatg tcctgaaata tgtttccctt gagctccatc atgaatttta gtcccaatag
     2521 attttgcttt aaaatcaaca ttgcgggttt gaaacatttc tccatctaca gactgagata
     2581 atcttgaagc agtgttatgc ttgtaggagt ctgagaaatc cccactaacc gcagccttcc
     2641 ccggttttgc cgcctttgtc aggtttttga ctttggcaaa cggcaggacg ttgaccaggg
     2701 cttcgacggt ttcggcggca ttggggtttt cctgcatcca ctggtcaacg gcttcgcccg
     2761 cgctgataaa ggggttgagc gcgccggcgg cga
//
LOCUS       DAXHPG010000200         2786 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXHPG010000200 DAXHPG010000000
VERSION     DAXHPG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.200"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            1..476
                     /locus_tag="ACFM6F_001988"
                     /pseudo
     CDS             1..476
                     /locus_tag="ACFM6F_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(523..1932)
                     /gene="dacB"
                     /locus_tag="ACFM6F_001989"
     CDS             complement(523..1932)
                     /gene="dacB"
                     /locus_tag="ACFM6F_001989"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC6165340.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLSHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGNGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            2097..2663
                     /locus_tag="ACFM6F_001990"
     CDS             2097..2663
                     /locus_tag="ACFM6F_001990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC6165341.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          672 a          650 c          766 g          698 t
ORIGIN      
        1 agatgaaagt tattttggcg gacaacgcaa aggcaaacgc ggtcgcggtg ctgccggtaa
       61 agtcgccgta ttcggtcttt tgaagcgaaa tggtaaggtt tatacggtta cagtaccgaa
      121 ttcaaaccgc tactttattt cctattatcc gtgaacaagt gaaacctgac agtattgttt
      181 atacggattg ttatcgtagc tatgatgtat tagatgtgag cgaatttagc cattttagct
      241 tcgctgaaac ttcgttttcg tatcaatcac agcacacatt ttgccgaacg acaaaaccat
      301 attaatggaa ttgagaactt ttggaaccag gcaaaacgtc atttacgcaa gtttaacggc
      361 attcccaaag cgcattttga gctgtattta aaggagtgcg aatggcgttt taacaacagt
      421 gagataaaag ttcttgttcc attttaaaac aattagtaaa atcgagtttg tcctagttat
      481 ctaggacagc cccttaaatt taatccacta tattttcctg tttcaggctc ggcgttcttt
      541 gcacatcagt ttcgcatcca gccagccatc gccgccggag atgatgttgt tggcaacgaa
      601 gttgtccaag tcgggcagca gggaaacggc gcggccgctg ttgatgatga cgaccaccgc
      661 catcggtttg tcgcccagcc aataacctgc aagggcgcgg acattgttga gcgtgccggt
      721 ttttaagcgc aacagcccgc cgctttgttt gaagcggttg cgtaaagtcc cgtctgtgcc
      781 ggcgatgggc agcgtatcga tgaaatcttg tgcaaacggg ctgaaataag ccgtttccaa
      841 catttgcgcc atcattctcg ccgttaccct ttcttttcta gacagacctg aaccgttttc
      901 caaaaccaaa tccgcaacat cgatgcccga cacggcaagt tcacgccgga cggcagacgc
      961 tgcctgttcg gaaacggcgg gcagtttgcc gttgccgccg agtttgagga agacggaacg
     1021 cgcaattaga ttgtccgaac gcttgttcat gtccgtcaaa atttccttca tcggtttggc
     1081 gtgtgcaacg gcaagcgtct gcgcgccttc cggcgtgtcg gctatgccga taccgtctga
     1141 aatccgtccg ccgccgagca gccagtggtt ggtaaaactt tgccggatca gttcgtcaag
     1201 cgcgaacatc cggacaccga caggcttgcc caaacagctc tcgggaatat tgccgcgcaa
     1261 tttcagcgta ttgtccgaaa aagatgcacg catcagtttt ttgatcgaag ggcaggcagc
     1321 ttgggaggcg gtaattttca agttgttttg ggcgaaaata tgcgacaaag gcggatcggt
     1381 gaggatgtcg gtactgtcgg cggcattgcg ttcggcgcgc accataacca taccggcaga
     1441 cagcatagtt ggattggggg gcgtcataaa cggcgaaccg ctgtcggctt cgaaatcgtc
     1501 ggggctgccg acttcgcccc acaggctgtg gtcgagcatc aggtgtcccg tgatattgcg
     1561 tatgccttgt tcgcgcaact ggcgttggac ggcaagcagg ttttcctgat tgaaaacggg
     1621 gtcgccgctg cccgcccaat acaggtttcc gtcaagcgtg ccgtcgttta ccgtaccgtt
     1681 gcttttaaac tcggtcgccc agcggtaatt gctgccgaag gttttgaagg cggcaaacgc
     1741 ggtaacgagt ttcattgtgg aggcggggtt gacggggaca tccgagcggt ggtcaatgat
     1801 gacttttccg ctgtcaagct cttggacata tacggcgatt tcgttttgcg gaatgcggcc
     1861 ggtatcgagc gcgtgtgcgg cgagggaggc gagaagcagc agcagggagg ccgctgtttt
     1921 ggggaaattc ataggtaaat ctgttacata aaaaaagcaa ttataaggca aagccggata
     1981 agtgggaacg ctgtggcggc gggccggctt gtttgcaggg gaaatcacat atataataat
     2041 cgctatcatt atgaaatgat tgagacgcac aaacttaata ttcaggagga atgattatgg
     2101 ctaagaaaat cagtattttg gtgggcagcc tgcgccgtgc ttcttttgcg cgcaaagtgg
     2161 cattgaatgc ggcggagatg ttccccgaag gctggcaggc ggaaatcgtc gaaatcggac
     2221 atctgccgct ttacaatttc gattatgacg accctgcggt ggaagatgtg ccgctgcccg
     2281 aaagctacac ggctttccgc gaaacgatta aggcttcgga cggcattttg ttcgttacgt
     2341 ccgaaaataa ccgcaccatt cccgcctgtt tgaaaaatgc ggtggacatc ggctcgaaac
     2401 cgaatgccga cgtggcgtgg aaaaacaaac cggccggtat catcagccat tccgtcggca
     2461 agatgggcgg ttacagttcg caaaaaaacc tgcgccttgc gctgtcttat ttcgatatgc
     2521 ccgtaaccgg acagccggaa gtgtttttgg gcaattcgcc gacgctgttt gatgaaaacg
     2581 gcaaattgat cgactcggca agggattttg ttcagtcata tattaatcag tttgtcggtt
     2641 tgattgaaag aaatgccaaa taaacaaacc aagtcgaaag gccgcaaaac cggttcgggt
     2701 tttgcggctt ttttatatgg gctgggcagc gtcaaggttt gccgtttgcc ggataatgcg
     2761 cggcaaatga ccggcgtgtt tatagc
//
LOCUS       DAXHPG010000201         2777 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXHPG010000201 DAXHPG010000000
VERSION     DAXHPG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.201"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..1350
                     /locus_tag="ACFM6F_001991"
     CDS             <1..1350
                     /locus_tag="ACFM6F_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFC6165342.1"
                     /translation="AAIGYATGGSGGAVVGANVDWHNRQLHPSERDWIRKNAQAFAKQ
                     EVGGREPTIDEIALAEKRLAQQAAKETDLFWMLTLEKVTDLSAQKFLRQAKGTFTNEN
                     GTQQQFFTTQGKQFMRPELFANEVYDLSFYKNHLQSPKNSSISKGTGELARRVTSSTW
                     DKAKQDPILTGLQIAASPVHLVRDVGKASWQCVSGFGQCLQQGKENFVETGSAIGTGT
                     ASFQLNDLKPIYGQDVRGAQAGLVALQTADGITTALGAAKALNVAGKGSRYFINEWAS
                     SPPSGSRAAQRGAVGNLTHSQQVLTDGSHIQRGGKLLPNIRYKAGEFDYLYETDTLGR
                     IKSFETDNLQLTQRDRLKHNSKTPDKVKGQDHAGHLIGDRFGGSPNLDNLVSQHKDVN
                     LSAYKVLENKWAEAIKSGKNVKIQMQIEYAAGTRHPTRFVGYYWIDGKPTRININNL"
     gene            1365..1811
                     /locus_tag="ACFM6F_001992"
     CDS             1365..1811
                     /locus_tag="ACFM6F_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165343.1"
                     /translation="MIFEDYFSELQADMVSICLEYVQGKADKIYIYAAFEPRVSGAGC
                     FFSVKGKLVQRHLVNQEIPEVNLDHQIQVLDILMEDLEKLNSICQKFNQPMPTEIKMI
                     YDVKKNSLSANYQYNLILSSESGLLALPQDVEDAWFEEIKKQMEDN"
     gene            1967..2317
                     /locus_tag="ACFM6F_001993"
     CDS             1967..2317
                     /locus_tag="ACFM6F_001993"
                     /inference="COORDINATES: protein motif:HMM:NF045464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6883 domain-containing protein"
                     /protein_id="HFC6165344.1"
                     /translation="MPTLRLLIPNADSVIIDTRKFTDYALNPNHDVGRHKARVFESAL
                     GYNQSNATELILKIRQGLKDNPARLGKKDQYGQRYTVDIPITGPNGKTAIVTTGWILV
                     PGSEIPKMTTVLVK"
     gene            2327..2518
                     /locus_tag="ACFM6F_001994"
     CDS             2327..2518
                     /locus_tag="ACFM6F_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HFC6165345.1"
                     /translation="MNLNDVVKLKVKQPNSNVPEGSIGTIIEVLQELPSAVFEVEFIN
                     KNGELIDTLTLAECDIEPA"
BASE COUNT          970 a          441 c          601 g          765 t
ORIGIN      
        1 gcggcaatcg gctatgcaac tggtggtagt ggtggtgctg tggtgggtgc gaatgtagat
       61 tggcataatc ggcagttgca tccgagtgaa agagattgga taagaaaaaa tgcacaagct
      121 tttgcgaaac aggaggtagg tggtagagaa cccactatag atgaaattgc attagcagaa
      181 aaacgactag cacaacaggc ggctaaagag acagatttgt tttggatgct aacattagaa
      241 aaagtaacag atttatcagc tcaaaaattc ctaagacaag caaaaggaac ttttacaaat
      301 gaaaatggta cgcagcaaca attttttact acgcaaggga agcaatttat gcgtcctgaa
      361 ttgtttgcta atgaagtata tgatctgtct ttttacaaaa accatttaca atctcctaaa
      421 aatagtagta tcagcaaggg tacaggagaa ttggctcgaa gagttacttc atctacttgg
      481 gataaagcaa aacaagatcc tattttgaca ggattgcaaa tagctgcctc ccctgtacat
      541 ttagttcggg atgtaggtaa ggcaagctgg cagtgcgtaa gcggctttgg acaatgtttg
      601 caacaaggaa aagagaattt tgtagaaaca ggttctgcaa ttggaacagg aactgcctct
      661 tttcaactaa acgaccttaa gccgatttat ggtcaagatg tacgtggtgc acaagcagga
      721 ttggttgcac tacaaacggc agatggaata actacggcat tgggtgcagc aaaagcatta
      781 aatgttgcag gaaaaggtag tcgctatttt ataaatgaat gggctagtag tccgcctagt
      841 ggttctcgtg ctgcacagcg tggggcagtg gggaatttaa cccattcgca acaagttttg
      901 acagatggtt cacatattca acgtggcgga aaacttttac ctaatattcg ctataaagca
      961 ggcgagtttg attatttata tgaaactgat actttaggta gaattaaaag ttttgaaaca
     1021 gataatttgc aattaacaca gagagataga ttaaagcata attcaaaaac tcctgataaa
     1081 gtcaaggggc aagaccatgc aggacattta attggagata gatttggagg ttcgcccaat
     1141 ttagataatt tagtgtcaca gcacaaagat gtaaacttaa gtgcgtataa agttttggag
     1201 aataaatggg cggaagcaat taaatcagga aaaaatgtta aaatccaaat gcaaattgaa
     1261 tatgcagcag gtacaagaca ccctacacga tttgttggat attattggat tgatggtaaa
     1321 ccaactagaa ttaatattaa taatttatga aacaaggatt ttttatgatt tttgaagatt
     1381 attttagtga attacaagca gatatggttt ctatttgttt ggaatatgtt caaggtaagg
     1441 ctgataaaat ttacatttat gcagcttttg agcctcgtgt tagtggtgct ggatgttttt
     1501 tctctgttaa aggaaaactt gtacaacgac atcttgttaa tcaagaaatt ccagaagtta
     1561 atcttgacca tcaaattcaa gtattagaca tacttatgga agatttagaa aaactaaatt
     1621 ctatttgtca gaaatttaac caacctatgc caactgaaat taaaatgatt tatgatgtaa
     1681 aaaagaatag cttatcagca aattatcaat ataatttaat cttgtcatct gaatctggat
     1741 tacttgcttt accgcaagat gttgaagatg catggtttga agaaattaag aagcaaatgg
     1801 aagataatta attcacagca agcgtagcaa gcgtagggtg ggcaactcgt tgcccacgca
     1861 gttcaatcgt atatgttaaa atgatgtttt aatgtgaaat atttcatttc aggctgcctg
     1921 aaaaaataaa ataacgtggc gtgtatcaaa ccgcgtgggc aacgagttgc ccaccctacg
     1981 cttgctaatc cctaacgctg attctgtcat aatcgatacc agaaaattta cagattatgc
     2041 attgaatcct aatcatgatg ttgggagaca taaagctaga gtttttgaat cagctctagg
     2101 atataatcaa tctaatgcaa ctgagctaat tttgaaaatt agacaaggat taaaagataa
     2161 ccctgcaaga ttaggaaaaa aagatcaata tgggcaaaga tatacagtag atattcctat
     2221 tactggacct aatgggaaaa cagccattgt aactacaggc tggattttag tgcctggatc
     2281 tgaaattccc aaaatgacaa ccgttttagt aaaataggtg ccaataatga atttaaatga
     2341 tgttgtaaag cttaaagtaa aacagcccaa tagtaatgta cctgaaggta gtataggtac
     2401 aataatcgaa gttcttcagg aattgccatc tgctgttttt gaagtggaat ttataaataa
     2461 aaatggcgaa ctaatcgata cattaacgct agcagaatgt gacatagagc cagcgtaacc
     2521 tgtgcaatta atgcgaactg ttacatggta gggtgggcaa ctcgttgccc acgcagttca
     2581 gtctcatgtg ctaaaactat gttttaaggc gaaagttttg gattgggcgc aagtgcgtcc
     2641 ataagcggca aaacactggg acagggcgca aaaaataaac ctcaaaacaa acacctgaca
     2701 agcgtagccg ataaaaacgg cgcaagttca tcagtgggtt atggcagcga ccaagacagt
     2761 caaagcagca tcacgaa
//
LOCUS       DAXHPG010000202         2660 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXHPG010000202 DAXHPG010000000
VERSION     DAXHPG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.202"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(122..1573)
                     /locus_tag="ACFM6F_001995"
     CDS             complement(122..1573)
                     /locus_tag="ACFM6F_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6165346.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1596..2216)
                     /locus_tag="ACFM6F_001996"
     CDS             complement(1596..2216)
                     /locus_tag="ACFM6F_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6165347.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            complement(2415..>2660)
                     /locus_tag="ACFM6F_001997"
     CDS             complement(2415..>2660)
                     /locus_tag="ACFM6F_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6165348.1"
                     /translation="LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGI
                     PKAHFELYLKECEWRFNNSEIKVQISILKQLVKSSLS"
BASE COUNT          826 a          623 c          610 g          601 t
ORIGIN      
        1 ataggaataa gaattcggga caaaatgttt aaaccatttt gtcccgactg ctgtgatgcg
       61 gtttttttga actagcagga cgttacctaa aacctgcgtt gcctgaaacc ctttaagacg
      121 gttaaatccc tctgccgtat ttgtattcta caatcatatt atcgtcggta atgacttccg
      181 ccccagccga aggttccgtc atctgaatca gcatacgaga gacaaccttt tgtgctgcag
      241 catccacggt gctgctgtca aatacgtgcc tgccgctttc cggccaaatc aaccgggaga
      301 gacgttgctt gagcagttct ttattaggga aaactaccgg ggttgccgag ccgactacca
      361 tatgcccata gcggtatgca tagggaatac tgtgtacggc ggtagcaaaa gcatgcgggc
      421 tgtgcgtggt attaaacatt acaataccat ccggggtaag gtggctttgc acctgtttta
      481 aaaattccgc actcaacagg ttggtggaat aggcacgcca gtaccaagtc gtattcatca
      541 aaatcaggtc gaatttttca tcaggatggc gacgcagcca tttcctaccg tcatccaata
      601 caatttcaac acgtttgtcc tgcaaaagcg gggcgatttg cggctcgtcc gcgataaggc
      661 tacggtatgc cggattgatt tccgcaacga tcatcgactg catttccgga atggcagaca
      721 agacgcgcgc ccacgaacct gtactcaatc caacgacgaa aatgcggcgt atgccagact
      781 tcagggaggg tagcagatag gcacgttcga tgccgttgac actattgaat acatcggtat
      841 tgtatgcgcc gtcgtataca ttcgccccat aaacaacctt atcaccatct ctatggtaaa
      901 ccgcaacaat gccgtgtttg ttttcaatca gcctatccgg acggtcagca atattttgaa
      961 agacagaatc cggcagtagg aacatgagga tgccgaacat tagggaaact gctaccgaca
     1021 ctgcattcag tcggagactt ttttggaaca gtgtacaaaa caaagggaca gcagcagaaa
     1081 tcaaacagat gagcaggtaa atctgttggg tggacaagaa atcaagtatc acaaagccga
     1141 taaggaccgg acccaatgca ctgccggcaa cgttggcgaa ataaacattg gaaacctgtc
     1201 gtccggattt gttgccatcc gtacccacat ggtgtacgag cgggaaaatc aaccctctga
     1261 cgacggcaga cagggtaatg aagataccgg cgtggtggac gaagccggaa aaacccgtca
     1321 acaaccacgc agcacccaaa atcaaaaagt cggcaatacc cgcccacaag aagcactgcc
     1381 cgataaaggg aatatcaaca aagcggctgc ggcaaatccg tttgccaaaa tacgcgccga
     1441 cggcgatacc ggtcagaaaa caggcaagag taaatgaaaa tgcctgaggc acggactgtg
     1501 ctgcgaacga aaacatcctt acccacaaga cttctatacc caagctcaat aagccgctaa
     1561 ggaaagaaag catataaatc aaactagtat tcggtttagt gttcactata tccatccttt
     1621 ctgtaacgca gccatactga agcagcaatc agaaggttaa agcaggctgt cagcgcaatg
     1681 gtttgggaga gggtaaaaaa gacgtagaaa aattcggcgg cggcaagcga tccgagtgcc
     1741 gcacccaaag tgttgaaaaa atataaggta ccgatagact cgccaacatt atgtattttc
     1801 cggttaaaaa aacaggtcag caagggcaag gtcgcgccca tcataaaggt aggaagcagc
     1861 aataagagga aattggcagc agcgatgatg ggcaaatcag cctcaactaa aagatgcccc
     1921 aagccggaaa tcagacccct gcttaccaaa ccgaacagac cgatggatac ttcagcgatg
     1981 caaaacaggg ggatgatact tgaaggaaaa cggtcagcaa tgcgtccacc gaaatacgca
     2041 cccacaccca agccgaccat aaatacagaa ataatgacag taatcgaact caaatcgata
     2101 cctatgtgac tgaatagaag cctctgccag ctgacctggt aaatcagggc gcagaatcca
     2161 gaggcgaaaa acaccagcga caatcctttc agggttttac ttgcggtcga attcatattt
     2221 tctattttca aataggcgga actataaatg aaatgtatca atgtgtaaac atttgtatta
     2281 accgtaggaa atcaccgtat tgctcaaact gcaggccggg aaaataaact cgtaagcccg
     2341 caaacggcgt atcactgttc aaactgctgt gcggcttcac ggtgttataa aaataatggg
     2401 gctgtcctgg acaactaaga taaactcgat tttactaatt gttttaaaat ggaaatttga
     2461 acttttatct cactgttgtt aaaacgccat tcgcactcct ttaaatacag ctcaaaatgc
     2521 gctttgggaa tgccgttaaa cttgcgtaaa tgacgttttg cccggttcca aaaattctca
     2581 attccattga tatggttttg tcgttcagca aaatgtgtgc tgtgattgat acggaaatgg
     2641 ctaaattcgc tcacatctaa
//
LOCUS       DAXHPG010000203         2613 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXHPG010000203 DAXHPG010000000
VERSION     DAXHPG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.203"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..335
                     /locus_tag="ACFM6F_001998"
     CDS             <1..335
                     /locus_tag="ACFM6F_001998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165349.1"
                     /translation="KTAENLCWGFGCREGKEFCKGLKQKQKPKVPSFPRRRESRPPTR
                     QESIGNNRNRTNLDSRFRGNDGGKVAVFPINSCRSSFLGWREIKQKRAYQKITMQRTV
                     LAILRFAP"
     gene            complement(410..2044)
                     /gene="groL"
                     /locus_tag="ACFM6F_001999"
     CDS             complement(410..2044)
                     /gene="groL"
                     /locus_tag="ACFM6F_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC6165350.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLEDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAMPDMGGMGGMGGMM"
     gene            complement(2137..2427)
                     /locus_tag="ACFM6F_002000"
     CDS             complement(2137..2427)
                     /locus_tag="ACFM6F_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC6165351.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT          534 a          697 c          690 g          692 t
ORIGIN      
        1 cgaaaacagc cgaaaacctg tgttggggtt tcggctgtcg ggagggaaag gaattttgca
       61 aaggtctcaa acaaaaacag aaacctaaag tcccgtcatt cccgcgcagg cgggaatcca
      121 gaccaccaac gcggcaggaa tctatcggaa ataaccgaaa ccggacgaac ctagattccc
      181 gctttcgcgg gaatgacggc ggaaaggttg ctgtttttcc gataaattcc tgccgctctt
      241 cgtttttggg atggcgggaa ataaaacaaa agcgcgcgta tcaaaaaata acaatgcaaa
      301 gaacggtgtt ggcaatttta aggtttgcgc cttagccgca cttattcgca aaaaaaccgc
      361 acggcgttga ccgtgcggct tgttgtctga aggcttcaga cggcattgct tacatcatgc
      421 cgcccatacc acccatgccg cccatatcag gcatagccgg tttgtcttca gggatttcag
      481 caatcatgca gtctgtggtc agcatcaggc cggcgataga cgcggcgtgt tgcagcgcgg
      541 aacgggttac tttggcgggg tcgagtacgc ccatttcgat catgtcgccg tattcgccgc
      601 tgccagcgtt gtaaccatag ttgcctttgc cttccaacac tttgttcaca accacgctgg
      661 gttcgccgcc tgcgttggca acgatttggc gcagcggaga ctcaacggcg cgcaagacga
      721 tttgtacgcc tgcgtcttgg tctgcattgc cggtgtgcag gttttccaga gcggcacggg
      781 cgcgcaacag ggctacgccg ccgcctgcga ccacgccttc ttcaacggct gcgcgggtag
      841 cgtgcagcgc gtcttccacg cggtcttttt tctctttcat ttcgacttcg gtagcggcgc
      901 cgactttgat cactgccacg ccgcctgcca atttagccac gcgctcttgc agtttttctt
      961 tgtcgtaatc gctggttgcg gtttcgattt gttggcggat ttcggcaaca cgcgcttcga
     1021 tttgggctgc gtcgccaaag ccgtcgatga tggtggtgtt ttctttaccg atttcgatgc
     1081 gtttggcttg acccaagtct tccaaagtcg ctttttccag agacaggccg acttcttcgg
     1141 aaatcaccac gccgccggtc aggatagcga tgtcttgcag catcgctttg cggcggtcgc
     1201 cgaagcccgg agctttgacg gcaacggttt tcaggatgcc tcggatgttg ttcaccacca
     1261 aagtcgccaa ggcttcgcct tctacgtctt cagcgataat caacagcgga cggctggctt
     1321 tcgccacttg ttccaaaaca ggcagcaggt cgcggatgtt gctgattttt ttgtcaaaca
     1381 gcaatacaaa cggattgtcc aaagcagcga tttgtttttc agcatcgttg atgaagtaag
     1441 gggacaggta gccgcggtcg aactgcatac cttcaaccac gtccagctcg ttttccaaag
     1501 atttgccgtc ttcaacggta atcacgcctt ctttgccgac tttttccatc gcttcggcga
     1561 taatcgcgcc gacttgttcg tcagagttgg cggaaatcga gccgacttgg gcgatttctt
     1621 tggaagtatc gcaaggtttg gcgatgtttt tcagctcgtc aaccaaagcg gcgacggctt
     1681 tgtcgatacc gcgtttcagg tcggtcggat tcatacctgc ggtaacatat ttcatgcctt
     1741 cggcaacgat ggattgtgcc agtacggtgg cggtagtcgt accgtcgccc gccacgtcgt
     1801 tggttttgga cgcaacttct ttcaccattt gcgcgcccat attttcaaac ttgtctttca
     1861 gttcgatttc tttggcgacg gttacgccgt ctttggtgat gtgcgggccg ccgaaagcgc
     1921 ggtcaaccac tacgttgcgg cctttgggac ccagggttac gcgcacggcg tttgccaaaa
     1981 tgttcacgcc gtttaccatt ttttggcgga cttcattgcc gaactgtacg tcttttgctg
     2041 ccatttcaat tctccaaaaa tcattaaaac tgtctgataa aaccgtttat gccgtctgaa
     2101 ggcggtttgc cgtttcagac ggcatcgtgt ccatatttat ttttcaacga tgccgaagat
     2161 gtcttcttcg cgcatcacca acagctcttc gccgtcggct ttgacggttt ggccgctgta
     2221 tttgccgaag atgattttgt cgccgacttt gacatccagc ggacggcgcg cgccgtcttt
     2281 accgattttg cccgcgccca cggcgatgac ttcgcccata tcgggttttt cggcggccgc
     2341 acccggcaaa acgatacccg atgcggtttt ttcttcagct tccaagcgtt tgacgacgac
     2401 gcggtcgtgt aaaggacgga tggtcatatt tatgctccga taaaatggtt tgaaaacaat
     2461 catctgcccg aacggttcgg acagattgaa gtggaaaccg ggctgccgcc tgccggccgc
     2521 ccgtatgaat cggcaaaatt agggtgtgtg cgggcaaatt caagtgaggc ggaaaaaatt
     2581 tatttccgcc gttttttata gtggactaaa ttt
//
LOCUS       DAXHPG010000204         2612 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXHPG010000204 DAXHPG010000000
VERSION     DAXHPG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.204"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(311..544)
                     /locus_tag="ACFM6F_002001"
     CDS             complement(311..544)
                     /locus_tag="ACFM6F_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC6165352.1"
                     /translation="MKLTLMFREYCSLCHKMRDELKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(710..910)
                     /locus_tag="ACFM6F_002002"
     CDS             complement(710..910)
                     /locus_tag="ACFM6F_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC6165353.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1082..1957)
                     /gene="xerD"
                     /locus_tag="ACFM6F_002003"
     CDS             complement(1082..1957)
                     /gene="xerD"
                     /locus_tag="ACFM6F_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC6165354.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVQHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2009..2449)
                     /locus_tag="ACFM6F_002004"
     CDS             complement(2009..2449)
                     /locus_tag="ACFM6F_002004"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC6165355.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
BASE COUNT          697 a          745 c          522 g          648 t
ORIGIN      
        1 taccgattac acaaacacat atcatcttta cacaatcatg cttccatcaa cagtaaaaca
       61 tgatatgatt gccaacaata acatctcaca ataaattttc taatttttat tgaaaaaatc
      121 aataaataag aattctccca accgacaaat aaaataaaga aagggtcaat atgcaacacc
      181 gtagaagatt gacaatttac caggcatcca aacgtgcttc ctttactggc aggtcatccg
      241 ccccgcaaaa acgtaaagaa cgttgatttg aaaaaaatgc cgtctgaagt cctgcttcag
      301 acggcatttt ttaccgttcg agaaactgtt tcaacctgtc ctcatccaaa aaccagtggc
      361 agatttcctc atctcccgcc aacaaaacgg gaaccagttc attgtatttt tcttccaaaa
      421 caggattttc atccacatcg accacttcca gcccgaaccc gtattcatcc tgaaaaggtt
      481 tgagttcgtc gcgcattttg tggcacaagc tgcaatattc acgaaacatc aaggtcaatt
      541 tcatccgcgt ttccttatct gtcaatttgc acacgccaaa gccttagacg cagcagaatc
      601 atggtctatt tgggaaaaaa caatgttttc aaggaagatg atactcaagt cctgccaaaa
      661 acagcaaaaa tgccgtctga acagttcaga cggcatttcc aaaaccgttt tacgcttgaa
      721 cgttgatacc cgccgtaacc gtcggttgcg cattgtgcgc cgtcagaaac gaagcggcaa
      781 gctccccgca gcagccgcag cagctcgcta cgcccaattg cgactgcaaa tcgcccattg
      841 tggtcgcgcc ggcggcgatg gtttccttga tttgatggtc ggtaacggca ttgcagatgc
      901 agacaaacat tttgtgctcc gtgtgttctc aaactatcgt accgatagcg gcttttatta
      961 tcgtatgcaa atataaataa aaacggttcg cattgcaagg tcggcataca cggtttgtct
     1021 tggtaatttt ttatcaagtt ttcatttccg attgatttct tattaaaccg aagcagaagc
     1081 ctcagtttcg ggaatggtgt tccttcacta caccctgcaa ccatacattg gcaacatggg
     1141 tgtaaatctg cgtcgtattc aaatcggcat gtcccaacat atcctgaacc acgcgcaaat
     1201 ccaatccgtg ctgcaccaag tgcgtggcaa aggcgtggcg caggctgtgc gggctgatgt
     1261 gcccgatgcc tgcctgactt gcatattctt tgacaatcat ccatgccaac tgacgagaaa
     1321 tgcccgtctt tttctgactg acaaacaatg cgtcgcaatt cctgcctttc agcagaagtg
     1381 ggcgtgcctc cgtataatag cgttccaccc aatacgccga ctcctgcccc atcgggacca
     1441 tcctctgctt atcgcccttg cccagcgcgg taatacagcc cctgtccaaa tccacattgc
     1501 cgaagttcag cccgaccgcc tcgctgacgc gcaagccggt cgcgtacatc aattcgagca
     1561 gtgccttgtc ccgcaaaccg tgtggcgtat cagtatccgg agtggcaagc agccgggaaa
     1621 tctgctgttc tgtaatcagt gtcggaatat tcttgtcgat tttgggcggt ttcagcaaac
     1681 gggtgggatt gtccgtcctt atgccttcac gctccatcca tatatacagg cgtttgcacg
     1741 ccgataatgc gcgcgcctgc gaactccgtt gctctccgtc aacataaacc gccgccgcca
     1801 aatccgcttc gtccgcatcc ttcagcattc tgcccgattg ggacaggcgg cgggcgattt
     1861 tttccaaatc gcgccggtaa ccgtttaaag tattctgact gagccgcctg tccaaccaca
     1921 gcgtttcaag cagcctgtcg atcaaacctt cttccatacc gttccaaaca aatgccgtct
     1981 gaatcttctt cagacggcat ggttcacatt atcgggaaag cgtttccaac acttcctgtg
     2041 catgacccgc cactttgact ttccgccatt catgggcgat ttctccatcc ttattcaaga
     2101 cgaacgtact gcgctcgata cctaacgact ctttcccgta cagtttcttc aatttgatga
     2161 catcaaacag gcggcacact gtttcatcct tgtcgctcaa cagctcgaac cggaaaccct
     2221 gcttggcgca aaaattctga tgcgccttta cgccgtcgcg ggaaataccg accacggtat
     2281 aacccaatgc ctcaaactgt tccaaacgcg cattgaaatc caagccttcc gtcgtacagc
     2341 ccggcgtact gtctttcgga taaaaataca cgaccaaagg cagatgttct gccgaatgaa
     2401 aatccgcacc gctgctcgaa ggcagggtaa attcatattt cacatccata gtcctactcc
     2461 cgatattccc attattcaaa acggcacgca gacgcaccgc cgcaattgcc aaaccaaccc
     2521 cgattctacc gccccaaagg acaaggattc aaccgccgga aacatccaaa ccgacacacg
     2581 acggcatgaa aaatatccat gtcaaaccac aa
//
LOCUS       DAXHPG010000205         2609 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXHPG010000205 DAXHPG010000000
VERSION     DAXHPG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.205"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(81..359)
                     /locus_tag="ACFM6F_002005"
     CDS             complement(81..359)
                     /locus_tag="ACFM6F_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165356.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            complement(366..584)
                     /locus_tag="ACFM6F_002006"
     CDS             complement(366..584)
                     /locus_tag="ACFM6F_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC6165357.1"
                     /translation="MKFINTCRKYGAKLAVVTAVPLALATQANATLPDTAKNALEAAK
                     ADGMEAGWIVVGIFAALFVFSIVKRVMK"
     gene            complement(658..855)
                     /locus_tag="ACFM6F_002007"
     CDS             complement(658..855)
                     /locus_tag="ACFM6F_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165358.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(860..1150)
                     /locus_tag="ACFM6F_002008"
     CDS             complement(860..1150)
                     /locus_tag="ACFM6F_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165359.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            complement(1195..2537)
                     /locus_tag="ACFM6F_002009"
                     /pseudo
     CDS             complement(1195..2537)
                     /locus_tag="ACFM6F_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
BASE COUNT          631 a          609 c          597 g          772 t
ORIGIN      
        1 aacttcatgc gctcaattct taaacagaac cgccccgatt aatacgggta cggaaacgcc
       61 gagataaaaa taaaaatcca tcatttcaaa acctttttca gcagggaaac aaagtaaacg
      121 gacgcgagga tgccgaatac tatccagcct gtttcaagac cgctttgcag gttgtctttc
      181 ggactgcatt ccgccaataa aagccttagc ggctgaccgt ccgacatctt ccacaggctg
      241 ccgttatatt ccggcctgac aatctgtccg ttttctttga ttcttggtac taccaagctg
      301 aaataaaggt tttcagcctg gtgcttctca agacatttat ttccgacttg gtagtacatg
      361 ccgtcttact tcatcactct cttaacgatg gaaaagacaa aaagcgcggc gaaaatgccc
      421 actacaatcc aaccggcttc cataccgtcg gcttttgcgg cttccaaagc gttttttgcc
      481 gtatcgggca acgttgcatt tgcctgtgtc gccaaagcca gcgggacagc tgttacaaca
      541 gccagttttg cgccgtattt acggcaggtg ttaataaatt tcatgatatt ttccttcaaa
      601 aagtgtttgg cggtaatgga tggagcgttt ttcagacgac cgccgaacat ccgaaaatca
      661 gtctttcaaa aatccgaata cgacaaattc gtattggttg ccgatttctt ccaaacctgc
      721 gttaatcgct tcttcgaagt cgtagaaata atcggcattg gtgattaatt tggtatgtcc
      781 gatgtcgccc gtttcaggag agagatacag aaagtcccct gttgatacgg actggacaac
      841 atagactttc tgcattcaat cagcctttct tcacgagttg aaaaccgatg actttcagtt
      901 tttgggtttt gcccgtagtg acgatttcta cgttcaggtt tgcttcgatc ggaaattggg
      961 cgtttcggaa ctgctcgaaa ttggcagagc cgccgaaatc gtattcagta gtagagctgc
     1021 ccaatgcgtt gccttgggag ctgtctaagg gtgtggcgac aatcaggcgg caatagtcga
     1081 agctcttgcc ttcgatttgt ccgttgaatt ttttaacgcc gacgatgtga ccttgaagtt
     1141 ggatgttcat tttttggttt ccttgtgtga ttaaacgtct ttcgggcaga cactttaagc
     1201 ccatgaaatc ggtagtcttg cgaatttgtc gtaaatgaag ttgttatagc tttcttcatt
     1261 gttgacgtgt ttttgctgtt caagctgttt ttcaagattc tcgtaatatt cgtacatata
     1321 gtaagggtct ttgtacggtt tgaatgcggg ctgttcatga atggcttgag ctttcaaaaa
     1381 ggcgcagtcg taggcttcgg gagccaaaga cttgggcagc ttgtgatgac tcggctcaat
     1441 cagttcaaac agtttggctt tgtccaattc gggaaaaatg aatttcagac cgtttgccgc
     1501 acgtccgaac tgttttttta cccattcaag gtagcggtcg gctgaaatga ccttatcttc
     1561 cttaaccgcg tgtatgcgcg ttgccttttg gacgaatcgt tcgcaaatcg gatatgcgcc
     1621 gccgaaatat tcgcccggat tctgcaaaac ttcgaaaggg ataacgatat cttttgcttt
     1681 gaattcaatt tcaaatcgcg tccatgtgct tgttttatcg cccaactgct tgcctttttc
     1741 atagacgcgg acatatttgg acgattcacg ggagccgata ccataggtct tgcctttggt
     1801 cattttggct tcatcgtctt cttcccaatc tgaccccaaa cattcgcctt ttggtttgac
     1861 gtgatgacag gtaaacatac ctttatttcg gtcttcacgg gcttggttcg ggctgtattc
     1921 gccgttgaaa aagtcttttg cgatgtcaac gcgtgtgatt tttgggtgga ttgcattagt
     1981 caggaatgcg aaaagtcgtg attcccagcc ttcttttgcg acgccgcaac cggtgccggt
     2041 cagttcgaaa agaatggtat tgttttgtcc gccaaaatgg acgcgaccgt ataggacgtc
     2101 ttccgaaccc atcaaccaac agcgctcata aaaacgaccg cccgagcctt tggactcttt
     2161 gtagagaccg aaaccgaaaa cttcttcggc gagcatggac gcggcgtgaa tgaaatcttc
     2221 atcttctaac agactgactc taacaccgta tttatcgaaa aaggtttttt catgaaatga
     2281 aaagctaatt tgatcaatga aagccgaatc tgatacaccg cgccgaagag gaacgcctaa
     2341 caggtttcct ttaccgtccg ttatgtacgt ttcgtaacat tcgaagactt cctgaaccct
     2401 gcctgccgtt tcggtttctg tacccccccg ttagataagg ggggaaggtt tgaagcggtt
     2461 gccggcttcc tgccgtccgt cggcgcgtcc gtcatcacgc cggcaaccgc ctttgtcatc
     2521 ccttgcttat cttccatggt gcgaatcctc aaaaacgggc aaaaaaaagc cctgttactt
     2581 gtagaaagta aagggcgtta atttttgtt
//
LOCUS       DAXHPG010000206         2581 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXHPG010000206 DAXHPG010000000
VERSION     DAXHPG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.206"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(197..268)
                     /locus_tag="ACFM6F_002010"
     tRNA            complement(197..268)
                     /locus_tag="ACFM6F_002010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(233..235),aa:Arg,seq:cct)
     gene            complement(288..734)
                     /locus_tag="ACFM6F_002011"
     CDS             complement(288..734)
                     /locus_tag="ACFM6F_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC6165360.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(739..1713)
                     /gene="ispB"
                     /locus_tag="ACFM6F_002012"
     CDS             complement(739..1713)
                     /gene="ispB"
                     /locus_tag="ACFM6F_002012"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFC6165361.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1937..2245
                     /gene="rplU"
                     /locus_tag="ACFM6F_002013"
     CDS             1937..2245
                     /gene="rplU"
                     /locus_tag="ACFM6F_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC6165362.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2270..2542
                     /gene="rpmA"
                     /locus_tag="ACFM6F_002014"
     CDS             2270..2542
                     /gene="rpmA"
                     /locus_tag="ACFM6F_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC6165363.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          758 a          694 c          575 g          554 t
ORIGIN      
        1 cataaaacaa cagatttcca agccggaaca tcttttacgg agcctgaaaa tcatttcatg
       61 attctaccgt cctaaaggtc ggtttttcaa gcaggaagaa aaagttttca gatggcaaaa
      121 aagccctcca acactgaaag gccttatatc ggaaacttcc cgcaacacgg gaaacagaca
      181 aatgcaaatc gtcaaacctc gccaacagga atcgaacctg tattttacgc ttaggaggca
      241 tacgttctat ccgttgaact atggcgagcc gaaatgaaaa ggagatttta accctttccg
      301 acgacaaaag acaagatgcc ggccgcaatc agcggcccga cagggatacc gcccataaat
      361 gccaccccga taaccgtccc gattaacagc cctgtaatta aaacaggctg ctgtcccatc
      421 aaaggcacgc cgcgtcccgc cagccaagcc acgaaaatac cgataaaaac ggcggatatc
      481 attttaaaat tcaaaaattc ggcaacggga ggaacctgcg cctttcctga aaccaacgga
      541 ctcaaaaccc ctatggtcaa aagaatgata ccgagattca acccatgctt ctcgaccaac
      601 gggacaaact gtaccaatgc cgtctgctgc atcagcagca atatggttgc cgagatggta
      661 atcgaattgt tgttgctgac cacccccaac agaatcagcg taaccagaaa caggggaaca
      721 aaactgaaat tcatcgcctc aagacaccct gaccaaagat tccttcgcca gctgaatcat
      781 ggcatccttc acttcgctgt cgggcagggc atccaaggcg gtaacggcac aatcgactgc
      841 tttgcgcgcc tcgcctatcg aatatgccaa agcatccgaa cggacgacat aatcgtggat
      901 tttctcaaaa tagctgcgat ctgcattttc caaagcagta cgcacatcgt tcgcaacctg
      961 ttcggaaccc tgacgcatca gataaatcaa aggcaaagtc ggttttcctt ccgccaaatc
     1021 gtcgccgacg tttttgccgg tttcttcggt ttcgccagaa tagtccagca catcgtcaat
     1081 aatttggaat gccgtaccga catacatacc gtagtctttc aacgcctgtt cgtgttcggg
     1141 ggaagccttg cccaaaattg cgccgacttg agcggcagct tcaaacaatt ttgccgtttt
     1201 atattggatg acttggatat attgttcttc ggtaatgtcc gtattgccga tgttcatcag
     1261 ctgcatgact tcgccctcgg caataatgtt ggttgcatcc gccatcactt ccaaaacgcg
     1321 catactgccc gaggcaacca tcagttgaaa ggcgcgcgtg tataaaaagt cgccaaccaa
     1381 cacagccgcc gcattgccga acagattgtt tgccgttgcc cgcccacggc gcaaatcgct
     1441 ttcatcgacg acatcgtcgt gcaggagggt ggaagtgtgg ataaactcga ccatcgccgc
     1501 cagcgaatac agtttctcgt catcataacc gacagcctta cccgccaaaa tcgtcataat
     1561 cggacgcagg cgtttgccgc ccgcgctgat gatatatgta ccgatttgcg aaatcagtgc
     1621 gacatcggat tgcaccgcac ggttgatgac ttcattgact ttggcaaggt cttcaggcag
     1681 atgtcgctgg aaatagggca gattctcgag catggcagac tctcaggttt acgtttccgt
     1741 caaacggcag ataccgcttg aaagctgatt caaatagcgg gcaattatat cagcaaagcc
     1801 atcagggtac atcttttcag acggcatatg gttatccgaa ccgcgcaaac tttgacaaaa
     1861 caggcaaata acaatacaat gccgagttcc gcacaatggt gtgcggatat taaccataac
     1921 ccttatggag ttgagtatgt acgcggtcgt aaaaaccggc ggcaaacagt ataaagtttc
     1981 cgtcggcgaa aaattgaaag tagaacagat accagccgaa ctcgacagcc aaatcgaact
     2041 gaccgaagtt ttgatgattg ctgacggcga atctgtaaaa gttggcgcac cctttatcga
     2101 aggtgcaaaa gtaacggcta aagtagtggc acacggtcgt ggcgaaaaag tccgcatctt
     2161 caaaatgcgc cgccgcaaac actaccaaaa acgccaaggc caccgccaaa atttcaccca
     2221 aatcgaaatc gtggcaatcg cctaacctta aaacttagga gcattacaaa tggcaagtaa
     2281 aaaagcaggc ggcagcaccc gcaacggtcg cgattcagaa gccaaacgct tgggcgttaa
     2341 agcctacggc aacgagctga ttcccgcagg ttccatcatc gtacgccaac gcggtaccaa
     2401 attccacgca ggcgacaacg taggcatggg caaagaccac actttgttcg ccaaagttga
     2461 cggttatgtc gaattcaaaa ccaaaggcgc gctgaaccgt aaaactgtca gcatccgtcc
     2521 ttacaccggt tctgaagaat aatccaaccg atacctgaag ccgcatcttt tcacgatgcg
     2581 g
//
LOCUS       DAXHPG010000207         2497 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXHPG010000207 DAXHPG010000000
VERSION     DAXHPG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.207"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            169..792
                     /gene="lolA"
                     /locus_tag="ACFM6F_002015"
     CDS             169..792
                     /gene="lolA"
                     /locus_tag="ACFM6F_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC6165364.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1065..2204
                     /locus_tag="ACFM6F_002016"
     CDS             1065..2204
                     /locus_tag="ACFM6F_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6165365.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAGQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTQQVKKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2336..>2497
                     /locus_tag="ACFM6F_002017"
     CDS             2336..>2497
                     /locus_tag="ACFM6F_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6165366.1"
                     /translation="MDLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT          675 a          724 c          616 g          482 t
ORIGIN      
        1 cggcacacat taaagggcag cgtggcgcgc ctgccttttc cgagcggtca aaaaaatcag
       61 ccctcggaaa acgctgtttg taaaatgcaa accgcccgta acgccgcccg tatgattgtt
      121 ttgccgcgcc gatactttac gccacaccca tcccaacaag gaaaaataat gatgaaaccg
      181 cacaacctgt tccaatttct cgccgtttgc tccctgaccg tctccgtcgc ttccgcacag
      241 gcgggcgcgg tggacgcgct caagcaattc aacaacgatg ccgacggtat cagcggcagc
      301 ttcacccaaa ccgtccaaag caaaaagaaa acccaaaccg cgcacggcac gttcaaaatc
      361 ctgcgcccgg gcctcttcaa atgggaatac acttcgccct acaaacagac tattgtcggc
      421 gacggtcaaa ccgtttggct ctacgatgtc gatttggcac aagtgaccaa gtcgtcccaa
      481 gaccaggcca taggcggcag ccccgccgcc atcctgtcga acaaaaccgc cctcgaaagc
      541 agctacacgc tgaaagagga cggttcgtcc aacggcatcg attatgtgct ggcaacgccc
      601 aaacgcaaca atgccggcta ccaatacatc cgcatcggct tcaaaggcgg caacctcgcc
      661 gccatgcagc ttaaagacag cttcggcaac caaacctcca tcagtttcgg cggtttgaat
      721 accaatcccc aactctcgcg cggcgcgttc aagtttaccc cgcccaaagg cgtggacgtg
      781 ttgagcaact gatgccgtcc gcctcgatgc cgtctgaatg ccgccgaggc ttcagacggc
      841 attttcacgc aggcggaaca atgtcccgca ttaccgcccg atcgggcatc ggaacggcaa
      901 acccgtgaaa attaacggtt gcgccccggt tgtttttgcc gtttaatgca aaccttgctg
      961 caccaagggc caagaaagcc gaccggccgc ccccacagct tccgatgcag gcggcccgtc
     1021 cgtccctgca atgtttttta tttttgaacg aaaggtcgaa aaccatgaaa aaaacactgg
     1081 tggcggcggc gatcctgagc ctcgccttga ctgcgtgcgg cggcggaagc gataccgccg
     1141 cccaaacccc ctccgccaag cccgaagccg gacaatcggg caaactcaat atctacaact
     1201 ggtcggatta tgtcgatccc gaaaccgtcg ccgcctttga aaaagaaacc ggcatcaaga
     1261 cgcgttccga ttattacgac agcaacgaaa cactggaggc aaaagtcctg accggcaaat
     1321 ccggctacga cctgaccgcg ccgtccatcg ccaacgtcgg ccggcaaatc aaagcgggtg
     1381 cgtatcagaa aatcgacaag gcgcaaatcc cccattacgg caacatcgat aaagatttgc
     1441 tgaaaatgat ggaagccgtc gatccgggca acgaatatgc cgtgccttat ttctggggaa
     1501 tcaatacttt ggcaatcaat acccagcagg tgaaaaaggc attgggtacg gacaagctac
     1561 ccgaaaacga gtgggatttg gtgttcaaac ccgaatacac cgccaaactc aaatcctgcg
     1621 gcatcagcta tttcgacagc gcaatcgaac agattccctt ggcgttgcac tatttgggca
     1681 aagaccccaa cagtgaaaat cctgaagaca tcaaagccgc cgtcgatatg atgaaagccg
     1741 tccggggcga cgtgaaacgc ttcagctctt ccggctatat cgacgatatg gcggcgggca
     1801 acctgtgtgc cgccattggt tacggcggcg atttgaacat tgccaaaacc cgtgccgaag
     1861 aagccgcaaa cggcgtggaa atcaaagtat tgaccccgaa aaccggcgtg ggcgtgtggg
     1921 tggattcctt tatgattccg cgcgacgcgc aaaacgtcgc caacgcccac cgctatatcg
     1981 actacacgct ccggcccgag gtggcggcga aaaacggcag cttcgttacc tacgcgcccg
     2041 ccagccgtcc cgcgcgcgag ctgatggatg aaaaatacac ctccgacgca tcgattttcc
     2101 cgaacaaaga actgatggaa aaaagtttca tcgtatcgcc caaatccgca gaatccgcca
     2161 aactgggcgt gaagctgtgg caagggctca aggccggaaa ataaccgcaa cctctgccgt
     2221 ctgaaacctt tcgggcggca ggaaacggcg cgtccgttat caaacagggg gcgtttcccc
     2281 gcccgcctgt tatgattggg ttaagattaa aatgatttag taaaatgaga aagatatgga
     2341 tttaagtatc gtagttccca tttataatgt cgagagttat ttggaagcgt gtctaaattc
     2401 catagaacct atattaagta atgaaaatgt cgaacttatc cttgtgaatg acgggtcaaa
     2461 agacggaagt gaagatatat gttataaata tatagat
//
LOCUS       DAXHPG010000208         2481 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXHPG010000208 DAXHPG010000000
VERSION     DAXHPG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.208"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(26..832)
                     /locus_tag="ACFM6F_002018"
     CDS             complement(26..832)
                     /locus_tag="ACFM6F_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC6165367.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILNR"
     gene            1052..2422
                     /gene="ffh"
                     /locus_tag="ACFM6F_002019"
     CDS             1052..2422
                     /gene="ffh"
                     /locus_tag="ACFM6F_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC6165368.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFINNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLHKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          680 a          630 c          646 g          525 t
ORIGIN      
        1 ctcagacggc atctgttggc tcttcttatc tgttcagaat gatttccaat acgaacttgc
       61 tgcccatata ggcaatcatc aggctgacaa atccgatgat ggtccacacg gcggcttttt
      121 tgccgcgcca tgcggtcatg ctgtgcttga gcagcagtcc gccgtaaatc agccatgaca
      181 atatgccgaa tacggtttta tgggtaaagg tcatgggttt gccgaatacg gcttcggcaa
      241 aaaatgttcc gctgacgacg gaataggtca gcaggatgaa acctgcccac atggcctgga
      301 acatgagttt ttccaaactg agcagcgacg gcaggaatcc tgcgagcttg gagaagctcc
      361 tgcggtgcag gctgcgattc agcagcaggg tcaaaacgga caataatgtt gcgatgccga
      421 acagcccgta tgcaagcagc gaagttccga tatgcagcat aaagggaagg tcggtaattt
      481 catatcccga gaattttcca ggaaaaacca aacctgacag cagcatcagt gcggcgcaag
      541 gatacagcag caactgcact ccgcgcagcg gataaaagaa gctgccggca aaataaataa
      601 acagcatcat ccaaacaatc aggctgccgg aatacccgaa gcccataatg atgattttgt
      661 cttgaatgac cggcatcagc agtgccgcgc cgtggacggt caatgccgcg cccaaaaccg
      721 gcaattccgt cttccacggg taatcccgac cgcgcccctg ctgttggcag tgccatgcaa
      781 atgcacccaa tcctgcgtaa accgccgtca aaaagatgaa aactgtcggc atggtggact
      841 ttctctatct atactgttgc gccgtctgcg gccgcttatg aaatattgga acttttaacg
      901 ttggaattgt aaaatccccg tttcgggcaa gccttgacgg atttgccgat atgctgtccg
      961 gcacacaagc cgcatcaaat tattttgatt ttattttaac aaagaatgcc cctgatgggg
     1021 caagctattc ttattcagac caaggaccag tatgttagac aatttaaccg gccgcttcag
     1081 caatgtcttc aaaaacatcc gggggcaggc caaactgacc gaagacaata ttaaagaggc
     1141 cttgcgcgaa gtccgcctcg ccctgcttga ggcggatgtc gccctgcctg tcgtcaaaga
     1201 gttcatcaac aacgtcaaag aaaaggccct cggtcaggaa gtagcgggca gcctgacgcc
     1261 ggatcaggca tttatcggcg tggtcaacaa agccctgacc gaactgatgg gcagggaaaa
     1321 caaaacgctg gatttgtcgg ttgcgccgcc cgccgtcgtg ttgatggcag gtttgcaggg
     1381 cgcaggcaag acgacgaccg tcggcaaact cgcccgcctg ttgaaaaacg atcagaagaa
     1441 aaaggttttg gtggtatccg ccgacgttta ccgtcctgcc gcgattgaac agctgcgtct
     1501 gttggccgaa caggtcggcg tggatttttt cccgtccgat accaaccaaa aaccggttga
     1561 aattgcaact gccgccgtcg attacgccaa aaaacatttt tacgatgtat tgatggtcga
     1621 taccgccggc cgtttggcaa tcgatgaaga gatgatgaac gaaatcaaag cccttcacgc
     1681 ggcggttaac ccggtggaaa ctttgttcgt catcgatgcg atgctgggtc aggatgcggt
     1741 gaacactgct caggcattta atgaagccct gccgctgacc ggtgtcgtat tgaccaagat
     1801 ggacggcgat tcgcgcggcg gcgcagcttt gtccgtgcgc cacgtaaccg gcaaaccgat
     1861 taaatttatc ggtgtcggcg aaaaaatcaa cggcctcgaa cctttccacc ccgaccgtct
     1921 tgccagccgt atcctcggca tgggcgacgt attgaccctg attgaagacg ttcaaaaagg
     1981 tattgatgaa gaagccgccg ccaaaatggc gaaaaagctg cacaaaggca aaggcttcga
     2041 cctcaacgac tttaaagaac aaatccagca aatgcgcaat atgggcggtt tggaaaacct
     2101 gatgtcgaaa atgccgggcg aactgggtca aatctcgaaa caaatccccg aaggaacggc
     2161 tgaaaaagcg atgggcaaag tagaagccat catcaactcg atgaccccta aagaacgcgc
     2221 caaccctgcc ctgctcaaag ccagccgcaa acgccgtatt gcaatgggtg cgggcacgac
     2281 cgtacaggaa gtgaacaaat tgctcaaaca gtttgaacaa atgcaacaaa tgatgaagat
     2341 gttcagcggc aacggcttgg gcaaactgat gcgtatggcg aaaggaatga agggaatgaa
     2401 aggaatgttc ccgggtttgt aagccgattt gaccgaaaac gccgtctaaa atttcagacg
     2461 gcgtttttgt tttatattct g
//
LOCUS       DAXHPG010000209         2442 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXHPG010000209 DAXHPG010000000
VERSION     DAXHPG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.209"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            299..733
                     /locus_tag="ACFM6F_002020"
     CDS             299..733
                     /locus_tag="ACFM6F_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165369.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            1009..1728
                     /locus_tag="ACFM6F_002021"
     CDS             1009..1728
                     /locus_tag="ACFM6F_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165370.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
     gene            1946..>2442
                     /locus_tag="ACFM6F_002022"
     CDS             1946..>2442
                     /locus_tag="ACFM6F_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6165371.1"
                     /translation="MIASRLSGNISSAIQEHKDGKVNINRFQNILADLYSLGGLGSTL
                     IEKNGNMQSWGIPLAIAGDIIAATAIATGDTGTISTEEFYNFDNWKGFGYELFEDWSR
                     WVYDWLPDGWNLWKELDRNRSGQYHIYDPLALDLDGDGIETVAAKGFSGSLFDHNGNG
                     IRTATG"
BASE COUNT          826 a          329 c          447 g          840 t
ORIGIN      
        1 caaacaaaaa agagaaattt taatttcgct aaatcgcata aagattaatc aagagtatca
       61 ttaaatgata tgagtgagca tctataatgc caagaagagt tgttaagaca taacgattat
      121 tgaaatagat tgtaaaatag atacttagat agtctgaaaa acggatttgt gaaacttttt
      181 attacgcgcc atcatttgaa aatgaaactt aaaaaacact tatcataata aatattttct
      241 ttacgttgtt tgctaataaa ctcagtgcaa tatcagcgca atattttatg gaaattttat
      301 ggataacaaa aaagaattta ttaataattt aacaaatagg tatatgtgga tctatccatt
      361 ggtcttaaat attctatttc taccttttta ccagtcctac caatcttttt ttattgcgct
      421 tggttgtttg tttgcactgg ttagaaaaat gcaaagctta gattttaaat tacaaaatca
      481 tattgtattg ttaaatataa aaagtgcttg ggcagataaa aaagtatttt tgattaggat
      541 agtagtgtca tggttggcag taatggaaat atggatgtgt tttatttcgg aatcatcaac
      601 gtgggtatgc ggtgcttttt gtttaaatag tgaaatattg gaaaaaattt ttcgtggctt
      661 tggttattct ggtagtttat attttttatt tatattgatg attgatctca ataagttaag
      721 agagagtatt tgaatttcat tttttgtttg acttaaactc aaggagagta acaatgattg
      781 gtagtggtga tactaaacaa tgcaaaaaat tttctgcgtg tgatggaaaa taccacgtct
      841 acgatcccct cgccctagac ttggacggcg acggcataga aacagtcacc gccaaaggct
      901 tttcaggcag cctgaagact gagagagtga atacgatgag tatacactct atgccactaa
      961 attgatattc actaaatcat accagctata ttttatttaa tgagacatat gaaaaataaa
     1021 aattatttac tagtatttat agttttacat atagccttga tagtaattaa tatagtgttt
     1081 ggttattttg tttttctatt tgattttttt gcgtttttgt tttttgcaaa cgtctttctt
     1141 gctgtaaatt tattattttt agaaaaaaac ataaaaaaca aattattgtt tttattgccg
     1201 atttctatta ttatatggat ggtaattcat attagtatga taaatataaa attttataaa
     1261 tttgagcatc aaataaagga acaaaatata tcctcgatta ctggggtgat aaaaccacat
     1321 gatagttata attatgttta tgactcaaat ggatatgcta aattaaaaga taatcataga
     1381 tatggtaggg taattagaga aacaccttat attgatgtag ttgcatctga tgttaaaaat
     1441 aaatccataa gattaagctt ggtttgtggt attcattcat atgctccatg tgccaatttt
     1501 ataaaatttg caaaaaaacc tgttaaaatt tatttttata atcaacctca aggagatttt
     1561 atagataatg taatatttga aattaatgat ggaaacaaaa gtttgtactt gttagataag
     1621 tataaaacat tttttcttat tgaaaacagt gtttgtatcg tattaattat tttatattta
     1681 aaatttaatt tgcttttata taggacttac ttcaatgagt tggaatagtt ttggtaattt
     1741 tatgagcgca cgctcatccg cgttagcaga atttggaaat atggttgcta atttagtttc
     1801 tgcaaaaaat gagaaagata tctcgaaacg taatgaatat tacaaacaag ctggttatag
     1861 tgcattatta gcatttggta atttggctag taatattgca ccaggtagta cgtcatcgca
     1921 tattgtaaac ggaacaaatg cctctgtgat tgcaagccgt ctctctggaa atatatcttc
     1981 agctattcag gagcataaag atggtaaagt taatatcaac cgttttcaaa atattttagc
     2041 ggatttatat tcattgggag ggttaggaag tacattaata gagaagaatg gaaatatgca
     2101 gagttggggg attccattag caattgctgg agatataatt gcagcaacgg ctattgccac
     2161 aggagatact ggtacgatat ctacagagga attttataat tttgacaact ggaaaggttt
     2221 tgggtatgag ctatttgaag actggtctcg ttgggtatac gactggctgc ccgacggctg
     2281 gaatctgtgg aaagaattgg acagaaaccg ttcaggccaa taccacatct acgaccccct
     2341 cgccctagac ctagacggcg acggcataga aacagtcgcc gccaaaggct tttcaggcag
     2401 cctcttcgac cataacggca acggcatccg caccgccacc gg
//
LOCUS       DAXHPG010000210         2438 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXHPG010000210 DAXHPG010000000
VERSION     DAXHPG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.210"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..372
                     /locus_tag="ACFM6F_002023"
     CDS             <1..372
                     /locus_tag="ACFM6F_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6165372.1"
                     /translation="AAANKGKCQDGAIGAAVGEILGEALLDGRDPGSLNVKDRAKIIA
                     KAKLAAGTVAALSKGDVNAAANAAAVAVESNALSKERMDKLTKCLSGKTCSTTMEKVN
                     AIKKDEQFSKVIDTEIQKSLF"
     gene            410..892
                     /locus_tag="ACFM6F_002024"
     CDS             410..892
                     /locus_tag="ACFM6F_002024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165373.1"
                     /translation="MSIKYIAMPAAWKYMPTDVSRVAKEVFGYLYNSQGASTRFDKYF
                     NTIDNRADFFAASNLYEQNLGSKARWFGGADFVSRAAITGLGADGEASYITFAAGKVV
                     GNPPIYEWRAASGNALIVNGFYNFRDLFNKKTNPREWDIQQLKSEQKLLQPIQPKIFE
                     "
     gene            1072..1506
                     /locus_tag="ACFM6F_002025"
     CDS             1072..1506
                     /locus_tag="ACFM6F_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165374.1"
                     /translation="MNKNYLFYLIQNIKSIFLIGGVFFGLLIFSGLWGNDFKLSFLGV
                     IDFIFGMVGGGFIVMLWFLIPVILSTLFRWVFQNQLAVSVILTFIVFYTYGYFSLFNS
                     SERVGSFWYLVIPTIMFIGLSEWFIYKSSVHSLGENVTPRSN"
     gene            complement(1574..1842)
                     /locus_tag="ACFM6F_002026"
                     /pseudo
     CDS             complement(1574..1842)
                     /locus_tag="ACFM6F_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244123.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1841..2014
                     /locus_tag="ACFM6F_002027"
     CDS             1841..2014
                     /locus_tag="ACFM6F_002027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165375.1"
                     /translation="MIKQVANNLPDGSPAKAAVFKAEREGKLKTAIAGVDRQTGKAVI
                     LSVKVPSKTNIRR"
     gene            2017..>2438
                     /locus_tag="ACFM6F_002028"
     CDS             2017..>2438
                     /locus_tag="ACFM6F_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6165376.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPEAIDYVDERKGVP
                     LAAMRHILMYTEAAASHAYLFEHDLNKFKQYAYVAGKLGILRSVNSTDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNR"
BASE COUNT          738 a          398 c          559 g          743 t
ORIGIN      
        1 gcggcggcga ataagggcaa gtgtcaggac ggcgcgatag gagcggcggt cggtgaaatc
       61 ctcggcgaag ccctactgga cggcagagac cctggcagcc tgaatgtgaa ggacagggca
      121 aaaatcattg ctaaggcgaa gctggcagca gggacggttg cggcgttgag caagggggat
      181 gtgaatgctg cggcgaatgc ggctgctgtg gcggtagaga gtaatgcgct tagcaaggaa
      241 agaatggata aattgacaaa atgcctttcc ggtaaaactt gttctactac gatggaaaaa
      301 gtaaatgcca tcaaaaagga tgaacaattt agcaaagtaa ttgatacgga aattcaaaaa
      361 agtctgttct aggaacccat tgggcgatgg ttgcagaaac ggcattaata tgtctattaa
      421 atatattgcc atgcctgctg cgtggaagta tatgcctacg gatgtatcac gggttgccaa
      481 agaagttttt ggctatttat ataactcaca aggggcatct acaagatttg acaagtattt
      541 caacaccatt gacaatcgtg cagatttctt tgctgccagc aatctttatg agcaaaattt
      601 gggttcaaaa gcacgatggt ttggtggagc tgattttgta tcgcgtgctg ctataactgg
      661 gttaggggca gacggagaag cttcttatat aacttttgcg gcaggtaaag ttgttggtaa
      721 tcctccaatt tatgaatgga gggctgcgtc aggcaatgca ctgatagtaa atggatttta
      781 taattttaga gacttgttca ataaaaaaac taatcctagg gagtgggata ttcaacagtt
      841 gaaaagcgag caaaagttat tacagcctat tcaaccaaaa atatttgagt aatgagaagg
      901 attatttgtc attgattaaa ggggtaacat caaataagat attttcaata attccaaatc
      961 ctttagatga gaggaaaaaa tagaggatgg aattaatatg ttagattaca aatctagaat
     1021 taaatatggt tgtaagctta tgggatattc tgagaaacag ggatgcaagc catgaataaa
     1081 aattatttat tttatcttat tcaaaatatt aaatctattt tcttaatagg tggagtattt
     1141 tttggccttt taattttttc aggtttatgg ggtaatgatt ttaaactatc ttttttaggg
     1201 gtaatagatt ttatatttgg gatggtaggg ggtgggttta tagttatgtt atggtttcta
     1261 attccagtga tattatcaac tttattcaga tgggtatttc aaaatcaact tgctgtatct
     1321 gtcatattaa ccttcattgt tttttataca tatgggtact tttctttatt taattcatct
     1381 gaaagagtcg gtagtttttg gtatttggta atcccaacca taatgtttat tggactgtct
     1441 gaatggttta tctataaatc tagcgtgcat agtttaggtg aaaatgtaac tccacgttcc
     1501 aattgaagaa aaagattgtc tgaaaactaa atttaatttc agatgacctt agattcggat
     1561 ttcaagtaca acactagggt atcgatggtt ggaacaaatt caagaataaa acacttggcg
     1621 tttcgtagcc aagtgttttt ctcggtcggt ggttcaactc atcttgaacc ctacgtatct
     1681 cccgatcgct gatgtttcgg aaaacggttt gtttggggaa tattgtcgga tgagtccatt
     1741 ggtgttttca ttcagccctt tctcccaaga atggtaaggg cggtaaaaat aggtttccgc
     1801 cttcaatgtt ttggccattt ggatgtgtcg gtagaattct ttgattaaac aagttgcaaa
     1861 taacttacct gatggtagtc ctgctaaagc tgctgtcttc aaagcggagc gggaaggcaa
     1921 attaaaaacg gcaatagcag gcgttgatcg tcaaacaggt aaggccgtta ttctttctgt
     1981 caaagttcct tctaaaacca atataaggag ataacaatgg ggcacaatat gatgaccacc
     2041 caaaaatggt atgagcatat tactaatgta atcataggca atactgctaa tttcaatagc
     2101 ggttgccctg aagctataga ttatgtagat gaaagaaaag gcgttccact tgcagcaatg
     2161 agacatattt taatgtacac tgaagctgcg gcttcccatg cctatctatt tgaacatgat
     2221 cttaataaat tcaagcaata tgcttatgtt gcaggaaagc tgggtatttt gcggagtgta
     2281 aattctacag accctgaacc cttcttcttt ccctgtgaca tgctcaacat tcaagatccg
     2341 atgtttctga tgctgatgag cgacagcccg cagctgcgtg agtttctggt gcgcaatatc
     2401 gacaacatcg ccaacgatac agaagccttc gtaaaccg
//
LOCUS       DAXHPG010000211         2433 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXHPG010000211 DAXHPG010000000
VERSION     DAXHPG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.211"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(78..153)
                     /locus_tag="ACFM6F_002029"
     tRNA            complement(78..153)
                     /locus_tag="ACFM6F_002029"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118..120),aa:Asn,seq:gtt)
     gene            complement(344..1096)
                     /locus_tag="ACFM6F_002030"
     CDS             complement(344..1096)
                     /locus_tag="ACFM6F_002030"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC6165377.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNTGCAPEEYVRIRRAIRLLEVRYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            1407..1931
                     /locus_tag="ACFM6F_002031"
     CDS             1407..1931
                     /locus_tag="ACFM6F_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFC6165378.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNAPPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHWVVADIPADVRRRN
                     AASLQLSRCANIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     TLCGTFSSASTAAA"
     gene            1892..2071
                     /locus_tag="ACFM6F_002032"
     CDS             1892..2071
                     /locus_tag="ACFM6F_002032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165379.1"
                     /translation="MRHFFIRQHRSRIIKRRAHPPGMRQLHRIAHCLRRPVLCGKPFY
                     GFLFESNPLRLIEVV"
     gene            complement(2156..>2433)
                     /locus_tag="ACFM6F_002033"
     CDS             complement(2156..>2433)
                     /locus_tag="ACFM6F_002033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165380.1"
                     /translation="TDGIWLKPGHSGQWDFGLFLGSNPFQIRVNRAAPYVRPCVLEII
                     VPSNYFHTRERLGALGGIVQHHTALYRSVAVYEPAVLIVRRGSKKLR"
BASE COUNT          561 a          747 c          595 g          530 t
ORIGIN      
        1 cctatcttgc cgcattccga acgccgtacg tgcaaatatg aaaaagcccg aacattcaag
       61 ttcgggcttc aaaattctgg ctccccgacc tgggctcgaa ccagggacct gcggattaac
      121 agtccgtcgc tctaccgact gagctatcgg ggaatggggt gtattatagc gtccgaaaaa
      181 aatgtgtcaa tccttaattt tggaaaaatg ggcgacaaaa cgacaagcat atgaatcaga
      241 aagacattaa gaccgatgcc ttaaaaggat tgccgttgta tgaatttcca cagccgtcat
      301 cacaccatat ttaagcccga tgagccgttc tgccctcccc cgcttaaaac aatgccgtct
      361 gaacttcgcc gcgttccaaa gccagtaaaa actgtttgcg gttcaacccg cccgcgtagc
      421 cggtcagttt gccgtcgctg ccgatgacgc ggtggcaggg aatcaggata gatactttgt
      481 tctgcccgtt ggcggcggca acggcgcgga cggctttggg gttgcccaaa cgctgcgcct
      541 gctccttgta gctgcgcgtt tcgccgtaag gaatcgccaa gagcgcgtcc catgcctgct
      601 tttgaaactc ggtgccaatc tgctccaaag gcgtggcaaa ggttttcaga cgacccttga
      661 agtataagtc caattcctgc cgcaaaagtt gcgtccgctc atcctcccga aacacaaacc
      721 gtccgcgcaa ggctttttgg acggcggcaa tttcctgttc caaatgcttc tgtccgacaa
      781 actccagcag acacaatccc ttatttccga acaccgccag catctcgccc aaaggcgtgg
      841 caatggcggc acacaccagc tcgttcaagc tgtcgggata acgcacttcc aacagacgga
      901 tggctcggcg gatacggaca tattcttcag gcgcgcagcc ggtattgtcc caaaaatccc
      961 gatcgaactg cccggcttcg cattccgtca gattgggatg cggcataacg ccgcactcaa
     1021 aaacccgggt ttcgaggcaa tggcggattt catcccattt tgacggcaga ttgtttaagg
     1081 aaggcagggt aatcattcgt ttgctccgta tctctatcat ggatttggcg gcaaaatccc
     1141 caattctcgt cattcccgca cgccgatgca ggaacgggct atgacgtaaa tcttgagggt
     1201 tgggttgcgg caatgcctaa atatgcgata tttctaaagc ctcagggaaa ggaattaaag
     1261 acttcagacg acctgcaaag ggtcgtctga aaccacgatt tttgcattta cgcattccgg
     1321 cacatcatcc aaccgtttcg gcacattcct gccgccgttg acagcctata atgaatccac
     1381 ttattcatca agcaaaggaa tcatctatgc aaacccgcat cctctccgcc gtactgctgg
     1441 ctttttcaac cgctgccttt gccgggggcg cattcacgct gcaattcgac aacccgtccg
     1501 aagacggcgg cttcacgcaa aaccagcttt tgagtgcgcc ttacggcttc ggctgttcgg
     1561 gcggcaatgc tccgcccgcg ctgtcgtgga aaaatccgcc cgccgggaca aaaagtttcg
     1621 tcctgaccgt ttacgataaa gacgcgccga ccggactggg ctggatgcac tgggtggtcg
     1681 ccgacattcc cgccgatgtc cgccgccgca acgcggcctc gctgcaatta agccgctgcg
     1741 ccaacatcgc cgacgaccag tccgcagcca tatcggcggt aatcagtttg cggatttgcc
     1801 gcatcaggtt gacgccttcg tacacggcaa aaccgatgcc gtcatgctgc aaccacgcca
     1861 acacgccgca aagcgcggcc tccgccacat tgtgcggcac tttttcatcc gccagcaccg
     1921 cagccgcata atcaaacgcc gcgcccatcc gcccgggatg cggcagcttc accgcatcgc
     1981 acactgcctg cgccgtcccg tattgtgcgg caaacctttc tatggcttcc tgttcgaaag
     2041 caatccattg cgcctgatag aggtcgtctg aatcgggaat attgatgacg tcaaacgtct
     2101 gcccgcctgc caaggcgacc gcctcacccg ccgcaatcct caattcgcgc ctcgctcacc
     2161 gcagcttctt gcttccgcgc cgcacgataa gcacagccgg ttcatatacc gccacgctgc
     2221 ggtacaaggc ggtatgatgt tgcacgatgc cgcccaaagc acccaatcgt tcgcgcgtat
     2281 gaaagtaatt tgacggcact attatctcca acacgcaggg ccgcacataa ggcgcagccc
     2341 tgtttaccct gatttggaag gggttacttc ccaaaaataa accaaaatcc cactgccccg
     2401 aatggccggg tttcaaccat attccgtcgg tgt
//
LOCUS       DAXHPG010000212         2432 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXHPG010000212 DAXHPG010000000
VERSION     DAXHPG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.212"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            35..1390
                     /locus_tag="ACFM6F_002034"
     CDS             35..1390
                     /locus_tag="ACFM6F_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HFC6165381.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQDAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="ACFM6F_002035"
     CDS             1394..2404
                     /locus_tag="ACFM6F_002035"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFC6165382.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVLDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          436 a          659 c          740 g          597 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcagatg agccgcatct tccagacgct
      181 cggtgccgat ccgcacagcc tcggctggtt ctttatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctat gggattcaaa gtttcttagc
      541 aaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggat
      781 cgaactcttg aaaaccgcgc ctaaggcgtt ttggacggtt actttggtgc aattcttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtttg
      901 gcacaccacc gatgcgtctt ccgtaggtta tcaggatgcg ggtaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttctgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat tttggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggttttag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaatggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgcat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg gc
//
LOCUS       DAXHPG010000213         2368 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXHPG010000213 DAXHPG010000000
VERSION     DAXHPG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.213"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            569..1384
                     /locus_tag="ACFM6F_002036"
     CDS             569..1384
                     /locus_tag="ACFM6F_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6165383.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHKLYYIKKGENPAIITHRNNRINQTEED
                     NYSTSVGSCINGFTVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
     gene            1401..>2368
                     /locus_tag="ACFM6F_002037"
     CDS             1401..>2368
                     /locus_tag="ACFM6F_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6165384.1"
                     /translation="MNEGEVVLTPEQIQTLYGYASRGDTYGGWRYLANLGDRYADNAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTYHGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSESDNRKQLISALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIETVAAKGFSGSLFDHNGNGIRTATG"
BASE COUNT          789 a          395 c          497 g          687 t
ORIGIN      
        1 caaaaatata ccaaaaacag caaaacaaat tgtaaggata cgtataggct ttgtaaaggt
       61 aaattgtgaa aaaagcagtt ttttaaacga atgaaacggc ttcgggctga aatatatgct
      121 gatgccctgt tcttcccgta tttctcgtgt gttgtcaaag tgcaggctgc tttgaaatcg
      181 gtattgccat ctatgaacca ccactttgct ttatttcagc gggcttgaga tgtgtataag
      241 gatattgttt tgaataaatt taaagaaaat gataatcgtt attgacgatt tttaaaggaa
      301 agcgtagagt gccaattcta tgaagcaata cggtaagtaa caatgaaaat atctactgct
      361 tgggtataga gcatatttca caacccgtaa ctattcttgc ggaaacagag aaaaaagttt
      421 ctcttctatc ttggataaat atatttaccc tcagtttagt taagtattgg aatttatacc
      481 taagtagtaa aagttagtaa attattttta actaaagagt tagtatctac cataatatat
      541 tctttaacta atttctaggc ttgaaattat gagaccatat gctactacca tttatcaact
      601 ttttattttg tttattggga gtgtttttac tatgacctca tgtgaacctg tgaatgaaaa
      661 gacagatcaa aaagcagtaa gtgcgcaaca ggctaaagaa caaaccagtt tcaacaatcc
      721 cgagccaatg acaggatttg aacatacggt tacatttgat tttcagggca ccaaaatggt
      781 tatcccctat ggctatcttg cacggtatac gcaagacaat gccacaaaat ggctttctga
      841 cacgcccggg caggatgctt actccattaa tttgatagag attagcgtct attacaaaaa
      901 aaccgaccaa ggctgggttc ttgagccata caaccagcaa aacaaagcac actttatcca
      961 atttctacgc gatggtttgg atagcgtgga cgatattgtt atccgaaaag atgcgtgtag
     1021 tttaagtacg actatgggag aaagattgct tacttacggg gttaaaaaaa tgccatctgc
     1081 ctatcctgaa tatgaagctt atgaagataa aagacatatt cctgaaaatc catattttca
     1141 taaactttac tatattaaaa aaggagaaaa tccggcgatt attactcatc ggaataatcg
     1201 aataaaccaa actgaagaag ataattatag cactagcgta ggttcctgta ttaacggttt
     1261 cacggtacgg tattacccgt ttattcggga aaagcagcag ctcacacagc aggagttggt
     1321 aggttatcac caacaagtag agcaattggt acagagtttt gtaaacaatc caagtaaaaa
     1381 ataatttaaa ggatcttatt atgaatgagg gtgaagttgt tttaacacca gaacaaatcc
     1441 aaaccttgta tggttatgct tctcgtggcg atacctatgg cggttggcgt tatttggcta
     1501 atttgggtga ccgttatgcg gataatgctg ccgcaattgt cggtaaggat gcaaacttaa
     1561 atggtttgaa tttatggatg aaaaaagggg tggaaaacct atgggatgat acggtcggta
     1621 aaaagacccg tttagagaaa tttgatcggg ttgcattgca acatttcagc caatatgtag
     1681 atctaattaa tgaaaataat ggtagattac ctaacactag tgaaattgag agaagttact
     1741 ataaagccgt tacctatcat ggcgtttctt ctagtgcagc tattgattta gttattaatc
     1801 gttcacttcc ggatatggcg gatggttatt gggcattagg tttggggata gaagccgaac
     1861 gtatccacaa tgagcaagca gtaaataatc cgaacggtag cgaatcggat aatagaaagc
     1921 agttaatatc tgctttagat aaaggatttg atggatcttt taaagagaag cattttactt
     1981 ttttacaatc tgtgataatg gatgtaacaa agttaggtgt tgaatataca atagatggtt
     2041 ggcaaaaaat tggaggttgg ggtaatggga taatcaatga tttatataaa agtgttgcaa
     2101 aaagagagtg gactggaata tttgagatcg ttaataataa catcaagcaa tttagagatc
     2161 tgttcccaaa tccggaaggc tggatcgatg atggtcacca atgtttcgct ccttgggtta
     2221 aagaaactaa aaaacgcaat ggcaaatatc atgtctacga ccccctcgcc ctagacctag
     2281 acggcgacgg catagaaaca gtcgccgcca aaggcttttc aggcagcctc ttcgaccata
     2341 acggcaacgg catccgcacc gccactgg
//
LOCUS       DAXHPG010000214         2339 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXHPG010000214 DAXHPG010000000
VERSION     DAXHPG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.214"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            44..2257
                     /locus_tag="ACFM6F_002038"
     CDS             44..2257
                     /locus_tag="ACFM6F_002038"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6165385.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          599 a          761 c          566 g          413 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac ggaagacccc atcatgaccg ccatcagccc
       61 gattcaagac acgcaaagcg cgactctgca agaattgcgc gaatggttcg acagctactg
      121 cgccgcgctg ccgaacaacg ataaaaaact tgtcttagcc gcccgttcgc tggcggaagc
      181 acattacccc gccgatgccg ccacgccgta tggcgagccg ctgcccgacc acttcctcgg
      241 cgcggcgcaa atggttcatg aactcgacct gctccccgat gccgtcgccg ccaccctgct
      301 tgccgacatc ggacgctacg tccccgactg gaacctattg gtttccgaac gctgcaacag
      361 taccgtcgcc gagctggtca aaggtgtgga cgaagtgcag aaactcaccc acttcgcccg
      421 ggtggacagc ctcgccacac cggaagaacg cgcccagcag gcagaaacta tgcggaaaat
      481 gctgctggcg atggttaccg acatccgcgt cgtgttaatc aaactggcga tgcgtacgcg
      541 caccctgcaa tttttaagca acgcccccga cagccccgaa aaacgcgccg tcgccaaaga
      601 aaccctcgac atcttcgccc cgctcgccaa ccgtttgggc gtgtggcagc tcaaatggca
      661 gctcgaagat ttgggcttcc gccatcaaga gcccgaaaaa taccgcgaaa ttgccctgct
      721 tttggacgaa aaacgtaccg aacgcctcga atacatcgaa aacttcctta atatcctgcg
      781 tacggaactc aaaaaataca atatccactt tgaagtcgcc ggccgtccga aacacatcta
      841 ctccatttac aaaaaaatgg tgaagaaaaa actcagcttc gacgggttgt tcgacatccg
      901 cgccgtgcgg attctggttg ataccgtccc cgagtgttac accacactgg gcattgtcca
      961 cagcctctgg cagcccattc ccggcgagtt cgacgactac atcgccaacc cgaaaggcaa
     1021 cggctataaa agtttgcaca ccgtcatcgt cggcccggaa gacaaaggcg tggaagtgca
     1081 aatccgcacc ttcgatatgc accaattcaa cgaattcggt gtcgccgcgc actggcgtta
     1141 caaagagggc ggcaaaggcg attccgccta cgaacaaaaa atcgcctggt tacgccaact
     1201 tttggactgg cgcgaaaaca tggcggaaag cggcaaggaa gacctcgccg ccgccttcaa
     1261 aaccgagctt ttcaacgaca cgatttatgt tttgaccccg cacggcaaag tcctctccct
     1321 gcccacaggc gcgaccccca tcgacttcgc ctacgccctg cacagcagca tcggcgaccg
     1381 ttgccgcggt gcgaaagtcg aagggcagat tgtgccgctg tccaccccgc tcgaaaacgg
     1441 acagcgtgtc gaaatcatta ccgccaaaga agggcatcct tccgtcaact ggctttacga
     1501 aggctgggtc aaatccaaca aggcaatcgg caaaatccgc gcctacatcc gccagcaaaa
     1561 cgccgacacc gtgcgcgaag aaggccgcgt ccaactcgac aaacagcttg ccaaactcac
     1621 gcccaaaccc aacctgcaag agcttgccga aaatctcggc tacaaaaagc cagaagacct
     1681 ctacaccgcc gtcggacaag gcgaaatttc caaccgcgcc atccaaaaag cctgcggcac
     1741 gctgaacgaa ccgccgcccg tacccgtcag cgaaaccacc atcgtcaaac agtccaaaat
     1801 caaaaaaggc ggcaaaaacg gcgtgctcat cgacggcgaa gacggtctga tgaccacgct
     1861 tgccaaatgc tgcaaacccg cgccgcccga cgacattgtc ggcttcgtta cccgcgatcg
     1921 cggcatttcg gtacaccgca aaacctgccc ctctttccga cacctcgccg aacacgcgcc
     1981 cgaaaaagta ctggacgcaa gttgggcggc gttgcaggaa ggacaagtgt ttgccgtcga
     2041 tatcgaaatc cgcgcccaag accgctccgg gcttttgcgc gacgtatccg acgcgctcgc
     2101 ccgccacaaa ctcaacgtta ccgccgtgca aacccagtcc cgcgacttgg aagccagtat
     2161 gcggttcacg ctcgaagtca aacaggtcaa cgacctcccg cgcgtcctcg ccagcctcgg
     2221 cgacgtcaaa ggcgtattga gcgttacccg gctttaaata caaaaatgcc gtctgaaagc
     2281 cgaataacgc ttcagacggc attttgattg ccggggtttg ctattttttg ttgcatagt
//
LOCUS       DAXHPG010000215         2313 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXHPG010000215 DAXHPG010000000
VERSION     DAXHPG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.215"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..275
                     /locus_tag="ACFM6F_002039"
     CDS             <1..275
                     /locus_tag="ACFM6F_002039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165386.1"
                     /translation="NPHHRHSRAGGNPKTQRNRNLSEKTETPPTVIPAKAGIQQHNAA
                     GIYRKKQKPPPTVIPAKAGIQQPKNHRNLSAKTETPTAVIPAKAGI"
     gene            477..2246
                     /locus_tag="ACFM6F_002040"
     CDS             477..2246
                     /locus_tag="ACFM6F_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFC6165387.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLS
                     ATVQANATDTDEDEELESVVRSALVLQFMIDKEGNGEIESTGDIGWSIYYDDHNTLHG
                     ATVTLKAGDNLKIKQSGKDFTYSLKKELKDLTSVETEKLSFGANGNKVNITSDTKGLN
                     FAKETAGTNGDPTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAASIKDVLNAGW
                     NIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIGAKTS
                     VIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTGQAD
                     KFETVTSGTKVTFASGNGTTATVSKDDQGNITVKYDVNVGDALNVNQLQNSGWNLDSK
                     AVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSVSLG
                     AGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLNNRI
                     DNVDGNARAGIAQAIATAGLAQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDTGNWV
                     IKGTASGNSRGHFGTSASVGYQW"
BASE COUNT          709 a          573 c          575 g          456 t
ORIGIN      
        1 gaaaccctca ccaccgtcat tcccgcgcag gcgggaatcc aaaaacgcaa cgcaacagga
       61 atttatcaga aaaaacagaa accccaccga ccgtcattcc cgcaaaagcg ggaatccagc
      121 aacacaacgc agcaggaatt tatcggaaaa aacagaaacc cccaccgacc gtcattcccg
      181 caaaagcggg aatccagcaa ccgaaaaacc acaggaatct atcagcaaaa acagaaaccc
      241 ccaccgccgt cattcccgca aaagcgggaa tctagacccg tcggcacgga aacttaccga
      301 ataaaacagt ttccttagat tccacgtcct agattcccgc cttcgcggga atgacgagat
      361 tttaagttgg gggaatttat cagaaaaccc ccaaccccca aaaaccgggc ggatgccgca
      421 ccatccgccc ccaaaccccg atttaaccat tcaaacaaac caaaagaaaa aacaaaatga
      481 acaaaatata ccgcatcatt tggaatagtg ccctcaatgc ctgggtagtc gtatccgagc
      541 tcacacgcaa ccacaccaaa cgcgcctccg caaccgtggc gaccgccgta ttggcgacac
      601 tgctgtccgc aacggttcag gcgaatgcta ccgataccga tgaagatgaa gagttagaat
      661 ccgtagtacg ctctgctctg gtgttgcaat tcatgatcga taaagaaggc aatggagaaa
      721 tcgaatctac aggagatata ggttggagta tatattacga cgatcacaac actctacacg
      781 gcgcaaccgt taccctcaaa gccggcgaca acctgaaaat caaacaaagc ggcaaagact
      841 tcacctactc gctgaaaaaa gagctgaaag acctgaccag tgttgaaact gaaaaattat
      901 cgtttggcgc aaacggtaat aaagtcaaca tcacaagcga caccaaaggc ttgaattttg
      961 cgaaagaaac ggctgggacg aacggcgacc ccacggttca tctgaacggt atcggttcga
     1021 ctttgaccga tacgcttgcg ggttcttctg cttctcacgt tgatgcgggt aaccaaagta
     1081 cacattacac tcgtgcagca agtattaagg atgtgttgaa tgcgggttgg aatattaagg
     1141 gtgttaaaac tggctcaaca actggtcaat cagaaaatgt cgatttcgtc cgcacttacg
     1201 acacagtcga gttcttgagc gcagatacga aaacaacgac tgttaatgtg gaaagcaaag
     1261 acaacggcaa gagaaccgaa gttaaaatcg gtgcgaagac ttctgttatt aaagaaaaag
     1321 acggtaagtt ggttactggt aaaggcaaag gcgagaatgg ttcttctaca gacgaaggcg
     1381 aaggcttagt gactgcaaaa gaagtgattg atgcagtaaa caaggctggt tggagaatga
     1441 aaacaacaac cgctaatggt caaacaggtc aagctgacaa gtttgaaacc gttacatcag
     1501 gcacaaaagt aacctttgct agtggtaatg gtacaactgc gactgtaagt aaagatgatc
     1561 aaggcaacat cactgttaag tatgatgtaa atgtcggcga tgccctaaac gtcaatcagc
     1621 tgcaaaacag cggttggaat ttggattcca aagcggttgc aggttcttcg ggcaaagtca
     1681 tcagcggcaa tgtttcgccg agcaagggaa agatggatga aaccgtcaac attaatgccg
     1741 gcaacaacat cgagattacc cgcaacggca aaaatatcga catcgccact tcgatgaccc
     1801 cgcaattttc cagcgtttcg ctcggcgcgg gggcggatgc gcccacttta agcgtggatg
     1861 acgagggcgc gttgaatgtc ggcagcaagg atgccaacaa acccgtccgc attaccaatg
     1921 tcgccccggg cgttaaagag ggggatgtta caaacgtcgc acaacttaaa ggtgtggcgc
     1981 aaaacttgaa caaccgcatc gacaatgtgg acggcaacgc gcgcgcgggt atcgcccaag
     2041 cgattgcaac cgcaggtttg gctcaggcct atttgcccgg caagagtatg atggcgatcg
     2101 gcggcggtac ttatcgcggc gaagccggtt acgccatcgg ctactcgagc atttctgaca
     2161 ctgggaattg ggttatcaag ggcacggctt ccggcaattc gcgcggtcat ttcggtactt
     2221 ccgcatctgt cggttatcag tggtaagggc tttttcgcct gtctgctgtt ggggcaggcg
     2281 gaaggtttga agggaagggt ggcgatttgc cgc
//
LOCUS       DAXHPG010000216         2312 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXHPG010000216 DAXHPG010000000
VERSION     DAXHPG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.216"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(24..455)
                     /locus_tag="ACFM6F_002041"
     CDS             complement(24..455)
                     /locus_tag="ACFM6F_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165388.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            complement(471..>2312)
                     /locus_tag="ACFM6F_002042"
     CDS             complement(471..>2312)
                     /locus_tag="ACFM6F_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6165389.1"
                     /translation="KGNLKTEIEKLSKQPEYAYLKQLQVAKNINWNQVQLAYDRWDYK
                     QEGLTEAGAAIIALAVTVVTSGAGTGAVLGLNGAAAAATDAAFASLASQASVSFINNK
                     GDVGKTLKELGRSSTVKNLVVAAATAGVADKIGASALNNVSDKQWINNLTVNLANAGS
                     AALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIVHKIAHAIAGCAA
                     AAANKGKCQDGAIGAAVGEIVGEALTNGKNPDTLTAKEREQILAYSKLVAGTVSGVVG
                     GDVNAAANAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVADKR
                     LAASIAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQAD
                     LALQSYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFVKQNTPIT
                     NVKYPEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQTDI
                     EGITRIKYEIPTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAASQGYSKA
                     SKIAQNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          570 a          492 c          448 g          802 t
ORIGIN      
        1 agcacacacc ttacgcatgg attttaggtt tcatgcaggc tacagtttgt ttgagaatat
       61 gttttttgta tttctttaca ctttttttga tattcagggt gagctattaa gaaatcagat
      121 attgcaatat taaagtaatg atatgctaaa ttaataggta taattaattc ttcaggattg
      181 aaatcatctc caactgaaaa ataaatagaa ggagctttat caatatactc aaatccattt
      241 acctcttcat aatctccaat ccatctggta gcatctattt tttcatattg agcacctatt
      301 actccaaaat gtccggtagt tgtaatattt ttaaaacact ctaaaagcgt atctttagtt
      361 tcaaattggt aaattgtttc aaaaaaaatc gaaataacaa aatggttgtt atttatagat
      421 tttttattta agtttagaaa aatattattt ttcataattt ttcctttaaa ttattctggg
      481 tgaatgtttg taattcttcc tgtatttaca tcaaaatatg atctaaattt gattccgtca
      541 aaggtttctg agaattgaat gacatttttt ctttccgata ttgatttagt tctttcattt
      601 tgagcaattt tagaggcttt tgaatatcct tgtgaagcag cattttgagc catttgaagt
      661 attttatcat cagaaaattt tttaggatta taaacagttt ttatacttga aatttcctta
      721 aatccaccat caggtttacc tgtcctgtct agtgtaggaa tctcatattt aattcgggta
      781 atgccttcaa tatcagtttg ggtttcagat tttacgcgtc ctcctcgtga atttagttct
      841 gccataaaat tggtgcggtt atgggctcct ttaatgccct gtttttgact aaaaccatca
      901 gcatttgcca gatgtctttt taggtttgta tcgaaactga tgccttccgg gtatttgaca
      961 ttagtaatag gtgtattttg ttttacaaac cctcttggta ttggaatgaa tctaggatta
     1021 actcctgaaa taagtgtata accttggtcg gacatccatt cggataaagg ctttgtattg
     1081 cccgattgaa gccaagattt agcagcagta ttcaaatgat aagactgtaa agccaaatct
     1141 gcttgggtgt aagatttgct gaataattta taccattcat catctttacc aattagacct
     1201 gcttcccaag atgaatgaag gcttctacta tcgatcaaat gttgttttct gattgttcta
     1261 cattcagtac tacgggatat atccgtacat attgcaatcg aagcagcaag tcttttatca
     1321 gcaacatttt gatacttttt tacagtattt tttctgcaca gttgaggatt attctgtttg
     1381 gcgcatgcag tcatttcgtt atcaaattct ctaccctctt tgtcgctaag ctgattattt
     1441 ttcaccgcta cctcagccgc attcgccgcc gcatttacat cgccgccgac cacaccgctt
     1501 accgtaccgg caaccagttt gctgtatgcc aaaatctgtt cgcgttcttt agctgtcaaa
     1561 gtgtcaggat ttttgccgtt tgtcaaagcc tccccgacta tctcgcccac agccgcacct
     1621 atcgcaccat cctgacactt gcccttattc gccgccgctg ccgcacagcc cgctatggca
     1681 tgggcaatct tgtggactat gtagtgctga tccaactgtt tgattttact ggctgcttct
     1741 ccatgcgcgg tattgaccaa agccgcaagg atattcgctt ccagattgtc tttcaggctg
     1801 ccgccgttga cagcggtatt aatcagtgcg gcactgcccg cattggctag gttgacggtc
     1861 aggttgttga tccactgctt atcgctgaca ttgttcagtg ccgaagcgcc gattttgtcg
     1921 gctacgcctg cggtagcggc ggcaaccacc agatttttca ccgtgctgct tctgcccagc
     1981 tctttcaggg ttttgccgac atcgcctttg ttgttgatga acgatacgga agcctggctg
     2041 gccaaagagg cgaatgctgc atcggttgcg gcggcggccg caccgtttaa tcccaatacg
     2101 gctccggttc ctgcgcctga ggtgaccacg gtaacggcca gtgcgataat cgccgcacct
     2161 gcttcggtta agccctcctg tttgtagtcc catctgtcgt aagcaagctg cacctgattc
     2221 cagttgatgt ttttcgctac ttggagctgt ttcagatagg catactcggg ctgtttggac
     2281 agcttttcga tttcggtttt cagattgcct tt
//
LOCUS       DAXHPG010000217         2309 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXHPG010000217 DAXHPG010000000
VERSION     DAXHPG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.217"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..567)
                     /locus_tag="ACFM6F_002043"
     CDS             complement(<1..567)
                     /locus_tag="ACFM6F_002043"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC6165390.1"
                     /translation="MEHKLPQLPYEPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAK"
     gene            731..2137
                     /gene="dnaB"
                     /locus_tag="ACFM6F_002044"
     CDS             731..2137
                     /gene="dnaB"
                     /locus_tag="ACFM6F_002044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC6165391.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          555 a          599 c          624 g          531 t
ORIGIN      
        1 tttggcgact tcgtcccagt tgacgatttc ccagaaacct ttcaggtagt tggggcggct
       61 gttgcggtag tcgatgtagt aggcgtgttc ccacacgtcg caggtcagca gcggcgtgtt
      121 ttcagtggtc agcggcgtag cggcgttgga agtggaaacc aaatccaatc cgccggcagg
      181 ggttttcacc agccatgccc aaccggagcc gaaagtaccg gccgcgcagg cattgaacgc
      241 ttcttggaat ttctcgaagc tgccccattt cgcgtcgatg gcggcggcca gttcgccgga
      301 aggtttgcct tgacctttgg gcgtgaaacc cagccagtag aaggtgtggt tccaagtttg
      361 tgccgcgttg ttgaacacgc cgcccgaaga ttttttcaca atctcttcca aaggcaggtt
      421 ttcaaattcg gtgcctttga tttgattgtt caggttggtg atgtaggttt gatggtgttt
      481 gccgtagtgg aattccaaag tctctttgct cagatgcggg gacaatgcgt ccggttcata
      541 aggcagttgc ggcagcttat gttccatttt gtgctcctaa tgttgttttt ggatgttgtg
      601 ttttggcagt gttgctgcaa acgaatcggc agcacacgca ttctacctgt tttgagggat
      661 ggaaaccaat tgaacctgct ttacgctata atagaagatt gcaatttcgg cacgacagat
      721 aggatgtacc atgaacgatt acgccgctat gccgtctgaa gaccgtgagg tcggcgcatt
      781 gtcgctgcct ccacattcga tggaggcgga acaatccgtt ttgggcggtt tgatgctgga
      841 aaatccggct tgggacagga ttgccgatgt ggtttccggt gaagacttct accgccacga
      901 acaccgcctg attttccgat ccattgctaa attaatcaat gagagccgtc ccgccgatgt
      961 gattacggtt caggaagatt tgcagcggaa cgaagaattg gaagcggcag gcggattcga
     1021 atatctgatt acgctggcgc aaaacacccc gtctgccgcc aacatccgcc gctacgccga
     1081 aatcgtgcgc gagcgttcca tcatgcgcca actcgccgaa gtggggacgg aaatcgcccg
     1141 cagcgcatac aatccgcaag gcagggacgc ggggcagctt ttggacgagg cggaaaacaa
     1201 agtattccaa atcgccgaaa gcaccgccaa atccaagcag ggctttttgg agatgcccga
     1261 tttgctgaaa gaagtcgtac agcgcatcga tatgctctac tcgcgcgaca atcccgatga
     1321 agttaccggc gtgccgacgg ggttcatcga cctcgacaaa aaaacctcgg gccttcagcc
     1381 cggcgacctg attattgttg ccggtcgtcc gtctatgggt aagaccgcct tttctatcaa
     1441 tatcgccgaa cacgttgccg tagaaggcaa gctgccggtt gccgtcttct caatggagat
     1501 gggcggggcg caactggtca tgcgtatgct cggctcggtc ggacggctgg atcaaagcgt
     1561 tttgaaaacc ggcaggctcg aagacgaaca ctggggtcgc ctgaacgaag cagtcgtcaa
     1621 actctccgac gcgcccgtgt acatcgacga gaccccgggt ctgaccgcgc tcgaactgcg
     1681 cgcccgtgcc cgccgtctcg cccgtcaatt taacaataag ctgggattaa tcgtcatcga
     1741 ctacctgcaa ctgatggcag gatccggccg ttccgacaac cgagcttcag agctgggcga
     1801 aatttcacgt tcgctcaaag cgttggcgaa agaattgcaa gtccccatca tcgccctgtc
     1861 gcaattgagc cgtaccgtcg aatcgcgtac cgacaaacgc ccgatgatgt ccgacctccg
     1921 cgaatccggt gctatcgagc aagatgccga cctgattatg ttcatgtacc gcgacgaata
     1981 ctacaaccag gactcaccca tgaaaggcct tgccgaatgt atcatcggca aacaccgcaa
     2041 cggtcccgtc ggtaaaatct tcctcacatg gacggggcaa ttcaccaaat tcgacaatgc
     2101 tgcctatatt cccgaggagg caaagataga ggattaaatg gctatataaa aatttattag
     2161 gcgaaatcag gcaaaatcgt ttaaaatcat gcttagagat tgccctaaaa aataaaacgc
     2221 ggtcttgagg catttttgca ttcagcccgc atataattga aaatatagtg gattaacaaa
     2281 aatcaggaca aggcaacgaa gccgcagac
//
LOCUS       DAXHPG010000218         2283 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXHPG010000218 DAXHPG010000000
VERSION     DAXHPG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.218"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            295..807
                     /locus_tag="ACFM6F_002045"
     CDS             295..807
                     /locus_tag="ACFM6F_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC6165392.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            887..1966
                     /gene="aroB"
                     /locus_tag="ACFM6F_002046"
     CDS             887..1966
                     /gene="aroB"
                     /locus_tag="ACFM6F_002046"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC6165393.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          562 a          709 c          548 g          464 t
ORIGIN      
        1 ttgataaaca tattatcttc aatatattca attggataat tgtttaccta agcaaagata
       61 attgcctttt cctgacaaat aagtgaaatc aacggattgt caaaacacag cctgaaataa
      121 aaaacctccc tgatttcttt tatttgtcct taaaatcaga aaggttcggg atggtcgggt
      181 tatttttcca aacgtaccgc cgccctgccg atttcgtata aaattccgcc gtaacccgac
      241 aagcccgaac cctgtcgccc cgaaaggcgg ggtgtcaaac attaaggaat tgtgatgaaa
      301 aactttaacg gcaaactcat cctcatcgga ctgatgggcg cgggcaaaac cacgctgggc
      361 cggcaaatgg cgcagcggct ggattaccgt ttttacgaca gcgatcacga aatcgccgca
      421 gccgccggcg ttcccatccc caccatattt gaaatggaag gcgaacaggg attccgttcg
      481 cgcgaaaccg ccatactcaa aaagctggtt atcctgcccc atatcgtcct gtccaccggc
      541 ggcggcgcgg tgttaaaaga agaaaaccgc gcccttatcc gcaaaagcgg cacggtcgtc
      601 tatctgcacg ccccgcccga aaccctgctc gaacgcacgc gctgcgacaa cagccgtcct
      661 ttgctgcaag ttgccgatcc tttggcgaaa ttacgtgaac tctacgccgc acgcgacccc
      721 gtttaccgcc aaaccgccga ctttaccgta gaatcggcaa actgccggga aaccgtgcaa
      781 accctgctca aacgcttatc ccgataaacc ggcatatgcg ccgcgcccag aaaaccaagc
      841 cgcgccccgc ccggcgggcc ggcggttcaa actttaagga acaacaatga aaacactgac
      901 cgtacacacc ccgtcccaca gctaccccat ctttatcggc aacggactgc tgccgcaggc
      961 aggaagcctg ctcaaaccgc atttgggcaa acgcgccgcc atcatcgcca acgaaaccgt
     1021 cgccccgctc tacctcggca cgcttcagac ggcattggat gcggcaggcg tatcccattt
     1081 cagcatcatc ctgcccgacg gcgaggcgca caaaaactgg cagacgctca acctcatctt
     1141 tgacgggctg atgcaaaacc gcgccgaacg caaaaccaca ttaatcgcac tgggcggcgg
     1201 cgtgatcggc gacatggtcg gctttgccgc tgccacctac cagcgcggcg caccgttcgt
     1261 tcaaataccg accacactgt tgagtcaagt cgactcatcg gtgggcggca aaaccgccat
     1321 caaccacccg ctcggcaaaa atatgatcgg cgcattctac cagccgcagg cggtgcttgc
     1381 cgacttggac acgctgcaca ccctgcccgt ccgcgaattg tccgccggta tggcggaagt
     1441 catcaaatac ggcgcgctcg gcgacatcgg ttttttcgaa tggctcgaac aaaacatatc
     1501 cggactgatg acgctcgatc gggaaaaact cgcccaagcc gtgtaccgct gctgccaaat
     1561 gaaggcagac atcgtcgccc aagacgaaac cgaacagggc atacgcgcat ggctcaacct
     1621 cggacacacc ttcggacacg ccattgaaac cgagatgggt tacggcactt ggctgcatgg
     1681 agaagccatc gccgccggct gcgtgttggc ggcgcgtttg tccgaacaac tgggcaaaac
     1741 ctccgccgca gataccgcgc ggctcgccgc cctgctcgaa gccgctggac tgccgtccgc
     1801 accgcccgtg tttgcctttg aaaaatggct cgaacatatg agccacgata aaaaagtcag
     1861 cggcggcatc atgcgcttta tcggtctgaa ccggctgggc gaagccaaca tcaccgaaat
     1921 taccgacacg gacatcctcc gccgcaccct gcaaccgtat ctctgatttc ctctgccgat
     1981 gtgctgccgc gcgggtttga cgcacgatga tgattttcca tcatctttct ccgtaaaagc
     2041 gggaatccgg tccgttcggt ttcggtcgtt tccgataagt tcccgttgct tttcatttct
     2101 agattcccac tttcgcagga atgacggtgg aaaggttttt gttgtttcgg agaagtttct
     2161 gcaaccctag aatctcgtta tttccacaaa aaacagaaac ccaaaacagc aacttaaaac
     2221 ctcgtcattc ccgcaaaagc gggaatccgg tccgttcggt ttcggtcgtt tccgataaat
     2281 tcc
//
LOCUS       DAXHPG010000219         2216 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXHPG010000219 DAXHPG010000000
VERSION     DAXHPG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.219"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            99..1910
                     /gene="typA"
                     /locus_tag="ACFM6F_002047"
     CDS             99..1910
                     /gene="typA"
                     /locus_tag="ACFM6F_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC6165394.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(1922..>2216)
                     /locus_tag="ACFM6F_002048"
     CDS             complement(1922..>2216)
                     /locus_tag="ACFM6F_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165395.1"
                     /translation="LRLRRLVLIFVNPLYPLPKLVRTQKAYSFKRHCSTCCREAEPDT
                     ETRRGDMPLEKWRQFLDWVGKMPSETASRSRKARPNKKCRLKFFRRHRVSFKH"
BASE COUNT          566 a          627 c          581 g          442 t
ORIGIN      
        1 gcaagggctt cagacggcat cttcatttcc caaatactgt ccggtaaagc gtggtataat
       61 gcgcagttat ctcttacagt ttttgaaaaa cattaattat gaaacaaatc cgcaacatcg
      121 ccatcatcgc ccacgtcgac cacggcaaaa ccacattggt cgaccaactg ctgcgccaat
      181 ccggcacatt ccgagccaac cagcaggttg acgagcgcgt gatggacagc aacgacctcg
      241 aaaaagaacg cggcatcacc atcctcgcca aaaacaccgc catcgattac gaaggctacc
      301 acatcaatat cgtcgatacg ccgggacacg ccgacttcgg cggcgaagta gagcgcgttt
      361 tggggatggt ggactgcgtc gtcttgttgg tggacgcgca ggaaggcccg atgccgcaaa
      421 cccgtttcgt gaccaaaaaa gccttggctt tggggctgaa accgattgtc gtcatcaaca
      481 aaatcgacaa gccgtccgct cgtccgagct gggtcatcga ccaaactttc gagctgttcg
      541 acaacttggg cgcgaccgac gagcagttgg atttcccgat tgtttacgct tcagggttga
      601 gcggtttcgc caaattggaa gaaaccgacg agagcaacga catgcgtccg ctgttcgata
      661 ctatcttaaa atatacgcct gcaccgagcg gcagcgcgga cgaaacgctg caactgcaaa
      721 tttcccaact cgactacgac aactacaccg gccgcctcgg tatcggtcgt atcttgaacg
      781 gacgcatcaa acccggccaa accgttgccg tcatgaacca cgatcagcaa atcgcccaag
      841 gccgcatcaa ccagcttttg ggtttcaaag gtttggaacg cgtgccgctt gaagaagccg
      901 aagccggcga catcgtgatt atttccggta tcgaagacat cggcatcggc gtaaccatca
      961 ctgataaaga taaccccaaa ggcctgccga tgttgagcgt ggacgaaccg acgctgacca
     1021 tggactttat ggttaacacc agcccgttgg cgggcacgga aggcaaattc gtaaccagcc
     1081 gccaaatccg cgaccgcctg caaaaagaat tgctgaccaa cgtcgccctg cgcgtggaag
     1141 ataccgccga tgccgacgtg ttccgcgtat ccgggcgcgg cgagctgcac ctgaccattt
     1201 tgctggaaaa catgcgccgc gaaggctacg aactcgccgt cggcaaaccg cgcgtcgtgt
     1261 accgcgacat cgacggtcaa aaatgcgaac cgtatgaaaa cctgaccgtg gacgtacccg
     1321 acgacaacca aggcgcggta atggaagaac tcggccgccg ccgtggcgaa ctgactaata
     1381 tggaaagcga cggcaacgga cgcacccgcc tcgaatacca tattccagcg cgcggcttga
     1441 tcggcttcca aggcgaattt atgaccctga cgcgcggggt cgggctgatg agccacgtgt
     1501 tcgacgatta cgcgcccgtc aaacccgata tgcctggccg ccacaacggc gtgctggtgt
     1561 cccaagagca gggcgaggca gtcgcttacg ccttgtggaa tctggaagac cgcggccgta
     1621 tgttcgtatc gcccaacgac aaaatctacg aaggtatgat tatcggcatc cacagtcgcg
     1681 acaacgattt ggtggtcaac ccgctcaaag gcaaaaaact taccaacatc cgtgccagcg
     1741 gtaccgacga agccgttcgc ctgaccacgc cgattaagct gacgctggaa ggtgcggtcg
     1801 agtttatcga cgatgatgag ctggtagaaa tcacgccgca atccatccgt ctgcgcaagc
     1861 gttacttgag cgaattggaa cgccgccgcc atttcaaaaa gctagattga tgtttactgg
     1921 attaatgttt aaatgatacg cgatgccgtc tgaaaaattt cagacggcat tttttattcg
     1981 gacgggcttt gcggcttctt gaagctgttt cagacggcat ttttcctacc caatcaagaa
     2041 actgccgcca tttttccagc ggcatatcgc cccgtcgcgt ttcggtatcg ggttcggctt
     2101 cccggcagca tgtcgaacaa tgccgtttga aggaatacgc cttttgagtc cttacaagct
     2161 taggaagagg atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcaga
//
LOCUS       DAXHPG010000220         2216 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXHPG010000220 DAXHPG010000000
VERSION     DAXHPG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.220"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            136..2016
                     /locus_tag="ACFM6F_002049"
     CDS             136..2016
                     /locus_tag="ACFM6F_002049"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HFC6165396.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELGKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVKPSDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDSK
                     FNVGAPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAITL
                     QKARGKYEPNRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVALAGKTAGN
                     AV"
     gene            2006..>2216
                     /locus_tag="ACFM6F_002050"
     CDS             2006..>2216
                     /locus_tag="ACFM6F_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilS"
                     /protein_id="HFC6165397.1"
                     /translation="MPSEARYGKLRQKQKPESHHSHSRESGFLGFRCFSVSMNFQAAV
                     PDKYPQSKIPSFPRRWESRFIRTETD"
BASE COUNT          601 a          674 c          518 g          423 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctctcc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccaattcaa aatcgccgcc
      181 atcatccaaa aaaacgatgc cacctaccaa gtcgataccc cacacggcaa acgcaccaaa
      241 gtgaaggcga acaacgtctt tgccgagttt gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggcac aggcggcgga catcgacacc gatttattgt gggaagtatg cggcgaagag
      361 gaatttaccg ccgaagccat cgccgaagaa tattacggcc atgcgccgac caaaaccgag
      421 ctggcggcaa ctttgattgc gctttacgcc gcgccgatgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaacc ttaaaacaag cgcttgccgc catcgaacgc
      541 aaaaaacagc aggacgcaca aatcgacgct tgggcagaag ccttgaaacg cggcgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcctgcacg cgcccgacaa gcagtcgctg
      661 acctacaaag cctttaccaa agccgccgac gcgctgaaaa cctctgccta cgaactgggg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacgggtt tgaaatcaaa
      781 tacttcccca aaggaacagg ctttcccgac cttccccttc ccgaaatgcc cgacctgccc
      841 aaagccgatg ttaccgcctt ttccattgac gacgaatcga ccaccgaagt ggacgatgcc
      901 ttgagcctga ccgacttggg caacggcacg aagcgcgtcg gtatccacat cgccgcgccg
      961 tcgcttgccg ttaaaccgag cgacaaaatg gaaaaaaaca tcatggaacg cttgagtacg
     1021 gtttatttcc ccggcggcaa aatcacgatg ctgcccgaaa actggattgc cgcgttcagc
     1081 cttgatgcag gcgcacaccg ccctgccgtc agcatttatt tcgatgtgga cagcaaattc
     1141 aacgtcggcg cgccgacctg caaaatcgaa gcggtcaaca tctccgaaaa cctgcgtatc
     1201 caagccatcg aaccgcattt caacgccgaa gcgggcttgg acgaagcggg cgaaatgatg
     1261 ttcgcccacc atcaagacct gatttggttt tatcaattcg ccatcaccct gcaaaaagcg
     1321 cgcggcaaat acgagcccaa ccgtgcgccg caatacgatt acagcatcga attggataaa
     1381 gaagacaacg tatccgtcgt ccgccgcgaa cgcggctcac ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcacaaatgc tccatgacaa cgacctgccc
     1501 ggcctcttcc gcgtccaacc caccggaaaa gtacgcatga gcaccaaatc cgagccgcat
     1561 atcggcatgg gcgtgcagca ttacggctgg tttacctcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgagcc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgcactg cgcgactttg atgccgccta taccgcctac
     1741 gccgatttcc aacggcagat ggaagcctac tggagccttg tgtacctgca acagcaaggt
     1801 acaagcgagc tgaccgcgac catcctcaaa gaagacctcg tccgcatcga aggcctgccg
     1861 ctggtcacgc gcgcgaccgg tattccgttt gacgcgctgc ccaaatcaca ggcattgttc
     1921 aaaatcaccg aattagatgc cgagaagcag tttgtctcgc tcaactacat caaggcagtc
     1981 gcactcgcag gcaaaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 aaaacagaaa cctgaatctc atcattccca cagtcgggaa tccggttttt tgggtttccg
     2101 ttgtttttcc gtttcaatga acttccaagc cgccgttccc gataaatacc cgcaatctaa
     2161 aatcccgtca ttcccacgca gatgggaatc taggtttatc cgcacagaaa ctgata
//
LOCUS       DAXHPG010000221         2193 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXHPG010000221 DAXHPG010000000
VERSION     DAXHPG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.221"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            30..515
                     /locus_tag="ACFM6F_002051"
     CDS             30..515
                     /locus_tag="ACFM6F_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165398.1"
                     /translation="MFVNEKDPYAALFAGLVFLTLPFALAVHDALAFVFGRTGLLVSV
                     SDGGFGWRGGWDGVVWFVYGVFAFLNLVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSGALGGLRGEAVLEYAFAVWLVAMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            611..1234
                     /gene="rsmG"
                     /locus_tag="ACFM6F_002052"
     CDS             611..1234
                     /gene="rsmG"
                     /locus_tag="ACFM6F_002052"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC6165399.1"
                     /translation="MERKERLRAGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLEAERHIVILSKR"
     gene            1333..2106
                     /locus_tag="ACFM6F_002053"
     CDS             1333..2106
                     /locus_tag="ACFM6F_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC6165400.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
BASE COUNT          422 a          503 c          761 g          507 t
ORIGIN      
        1 tcggtctgct tcggaagagg gggacggcga tgtttgtgaa cgagaaagat ccttatgcgg
       61 ctctgtttgc gggactggtg tttttgacgc tgccgtttgc gttggcggtg catgatgcct
      121 tggcatttgt gttcggacgg acggggctgc tggtgtcggt gtcggacggc ggattcggct
      181 ggcgaggagg ctgggacggc gtggtgtggt ttgtgtatgg cgtgtttgcg tttttgaacc
      241 tggtcgtgtc ggcgggcctg acgaaactgg cgtacaaaaa gatgatgcgg cggcattccc
      301 gttacgcgct gtttctgtcg ggcgtggcgg cttgcgcggc tgctgcggtc gcgtggattt
      361 tcgagctgct gcttggcagt ggggctttgg gcgggctgcg tggggaggcg gtgttggaat
      421 atgcgtttgc cgtgtggctg gtggcgatgc tgacgctgcc caaacgcctg acgcgcgcgc
      481 cggtgcagcc ggtggtgttt cacaggaaaa aataggttgg aacgggaaat gccgtctgaa
      541 ccccgacacg cggtttcaga cggcatgttt ttccgttaac attacgcctg aacatggaca
      601 ggatagggat atggaacgca aagaacgcct gcgtgcaggc atcgccgcat tgggtttgga
      661 tatttccgca gcggcgcagg acaggctttt ggcctatgtg gatttgttga aaaagtggaa
      721 caaaacctac aatctgaccg ccctgcgcga cgaggaaaaa atgattgtcc atcatctttt
      781 ggacagcctg acgctgctgc cccatatcga gggtgtgcaa acgatgctgg atgtcggttc
      841 gggcggcggt cagcccggca ttccggcggc ggtgtgccgt ccggatgtgc agataaccct
      901 tttggatgcg aatacgaaga aaacggcttt tttacagcag gcggttatcg agttgggatt
      961 ggacaatgtg cgcgtggtat ccggacgcgt ggaggcggtt tcggacgtgc gtgccgatgt
     1021 ggttaccagc cgtgcgtttg cagaactggc ggattttgtg tcgtggacgg tgcatctgtt
     1081 gaaagacggc ggctactggg cggcgatgaa gggcgtgtat ccgcaggaag aaatcggccg
     1141 cctgccgcag gatgtgtgcg ttgaaaaagt ccaaaggctc gacgtgccgg gcttggaggc
     1201 ggaacgccat atcgttatcc tgagcaagcg ttgagcgcgc ttcagacggc atgaatacct
     1261 tttttgtgcg gataaaggta aaattccgca ctgtttttct tttttcaaca tcagacggga
     1321 cacgggcggg acatgagtgc gaacatcctt gccatcgcca atcagaaggg cggtgtgggc
     1381 aaaacgacga cgacggtaaa tttggcggct tcgctggcat cgcgcggcaa acgcgtgctg
     1441 gtggtcgatt tggatccgca gggcaatgcg acgacaggca gcggcatcga caaggcgggt
     1501 ttgcagtccg gcgtttatca ggtcttattg ggcgatgcgg acgtgaaatc ggcggcggta
     1561 cgcagcaaag agggcggata cgctgtgttg ggtgcgaacc gcgcgctggc cggcgcggaa
     1621 atcgagctgg tgcaggaaat cgcccgggaa gtgcgtttga aaaacgcgct caaggcagtg
     1681 gcggaagatt acgactttat cctgatcgac tgtccgcctt cgctgacgct gttgacgctt
     1741 aacggcttgg tggcggcggg cggcgtgatt gtgccgatgt tgtgcgaata ttacgcgctg
     1801 gaagggattt ccgatttgat tgcgaccgtg cgcaaaatcc gtcaggcggt caatcccgat
     1861 ttggacatca cgggcatcgt gcgcacgatg tacgacagcc gcagcaggct ggttgccgaa
     1921 gtcagcgaac agttgcgcag ccatttcggg gatttgcttt ttgaaaccgc catcccgcgc
     1981 aatatccgcc ttgcagaagc gccgagccac ggtatgccgg ttatggctta tgatgcgcag
     2041 gcaaagggtg ccaaggcgta tcttgccttg gcggacgagc tggcggcgag ggtgtcgggg
     2101 aaataggtca atccaaatcg ggctgcccgt gcctttatgc tgtttggccg ggtgcgttaa
     2161 tatggcggat taaaataaaa atacttatat cgt
//
LOCUS       DAXHPG010000222         2148 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXHPG010000222 DAXHPG010000000
VERSION     DAXHPG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.222"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(150..665)
                     /locus_tag="ACFM6F_002054"
     CDS             complement(150..665)
                     /locus_tag="ACFM6F_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165401.1"
                     /translation="MSAFRKILLIISCLLIASCSFVETIFYMAISPEPVVVDFPLGKK
                     TKRSIELKQKIGKPYAISLGTNFIHYDPKQGERWIDDKLNYPYNISVKIFKVEEDGKK
                     LIIDELLTERSRKLGGGVFGAGGKYSMHIYDFYLPEGEYLFEISDNSEYIPLYDEINN
                     SIRIVVNARIQ"
     gene            complement(925..1195)
                     /locus_tag="ACFM6F_002055"
                     /pseudo
     CDS             complement(925..1195)
                     /locus_tag="ACFM6F_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248307.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein frpA"
BASE COUNT          739 a          369 c          276 g          764 t
ORIGIN      
        1 atttttttat ctctagccga atacagttca acccaaacgt acccgtcttg gatacacccc
       61 ctcttgtttc agtggcgtac cctgcttcgc aaattttaca gttatcgcat aataatcagc
      121 cataagtaac cccacatttc tagaaaaatt tactgaattc gtgcattaac tactattctt
      181 atagaattat ttatttcatc gtaaagtgga atatattcac tattatcaga aatctcaaat
      241 aaatattccc cttcaggcaa ataaaaatca taaatatgca tactatattt ccccccagct
      301 ccaaatactc cgcctcctaa ttttctactt ctctctgtaa gcaactcatc tataataagt
      361 tttttaccat cttcttccac tttaaatatt ttaaccgata tattatatgg atagtttaac
      421 ttatcatcaa tccacctctc cccctgtttt ggatcataat gtataaaatt agttcctaac
      481 gatattgcat aaggtttacc aattttctgt ttgagttcaa tagatctttt tgttttttta
      541 ccaagaggaa agtctaccac aacaggttct gggctaatag ccatataaaa aatagtttca
      601 acaaaactgc agctagcaat caataggcaa gatattatta ataatatttt tctaaatgca
      661 gacataataa aaatctccaa aaaatccata tcagaatata ctaattattt gcataattaa
      721 taaggaagag aaaaattcaa aacggctatg agatttccgc ctgaaaataa tgaaataaaa
      781 gtccatagta atgaataata gctttatcag ccccccttta atgcaaacat agagaatact
      841 tcccttgaag cactatcaat ttctacttaa ctaaaactct agctctattt taaaattttg
      901 tcaaaaatag ttaaatactt ttatttattt taaataatga gatacattta tggttatttg
      961 ttttagacag cctaaaacaa acggggaaat cgtgttttcc cgaacatggc taatggatag
     1021 cattttgtcc gtgaggaggc tgtgcaggtt tgtcagcagc caacagcgaa tcgcctgctt
     1081 gcgcggttgt gccgtctttt ttggtgtagc tgccttgttg agccaaagtg ttgccgttac
     1141 caagattttt gtttacatct tttcgagaaa attggctttg catttgcctt aacaaagata
     1201 tttctgctac tcgtgcttta ttatttatat tatatttact ctgcattttt cttacaactt
     1261 gtctcaactg actttgtgtt ttttgtaatt cacgttgtac cgattttcct aaactttcat
     1321 gtttggctgc ataattggta gaatcagttg acatttgtgt ataaaaattt ttacgaaaat
     1381 tttcgtaact cagattaatt acatccgtac ttgcagttat aaaatcttgt ataaattcaa
     1441 tagaaactga attatcatca gatttggctt tgttattttc catattgagc ctctcttaat
     1501 ttgaaaaaat taacataaac atttaaaaat aaaacaaaat tatataataa aaaaaataat
     1561 gagaatataa aggataaaaa gagagttaag ttatttaatc ggacttgttc tctataaatc
     1621 ataaggcacc aaataagcca taataaattt agtaaataaa aactgcttat acccaacttt
     1681 cttttcttgg aacctattat tatttcaact ctggatgaaa aaatagaatt taaaaatttt
     1741 tttctttgat atatcgttaa tatctcccta gtttttctta aaaaaatata atcatatata
     1801 tactacacaa cattggtaat aaataaatta aaagcgaaag gtaattaaat tgataattgt
     1861 aaattatata ataaacaaca tacgaagaaa aaattattat agatgatttg ctatacgaat
     1921 aaaaaaatat cctacctagc ccgaaccttc ttataatttg tactatgtga tcattcattt
     1981 caatatccat ttaagctttc aattaatttt ctatattttt caatggcatg tatcatttgg
     2041 ttgatgatca agccatgtat gcaaagattg gaatatggta tctgtcgtgc taataatgcc
     2101 cccgttactg tttaatttgc gcacaggaaa accgtcatat gcagcaat
//
LOCUS       DAXHPG010000223         2098 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXHPG010000223 DAXHPG010000000
VERSION     DAXHPG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.223"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(91..1080)
                     /gene="pdxA"
                     /locus_tag="ACFM6F_002056"
     CDS             complement(91..1080)
                     /gene="pdxA"
                     /locus_tag="ACFM6F_002056"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC6165402.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1240..>2098
                     /locus_tag="ACFM6F_002057"
     CDS             1240..>2098
                     /locus_tag="ACFM6F_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6165403.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMG"
BASE COUNT          402 a          565 c          633 g          498 t
ORIGIN      
        1 gcccgaaggt ctggattccc gcctgcgcgg gaatgacggc ggagggtgga cgatgccgcc
       61 tgcgcggaaa tgacgggtct tttatcatct ttaaaggctg ccgcgcgcca tctcgacggc
      121 ggtctccacg gcagttatca ggctgccgga atccgccctg ccggttgccg ccaaatcgag
      181 cgcggtgccg tgatcgacag aggtgcggat aaagggcaga cccagcgtga tgttcacgcc
      241 ctgcccgaaa ctgtggtatt tcaacacggg cagcccttgg tcgtggtaca tcgccaatac
      301 ggcatccgca ccttcgagca taaacggctg gaacaatgtg tccgccggat acgggccggc
      361 aaggtttatc ccttcgcggc gcaggttttc caatgcaggg ataatggtgt cggtttcttc
      421 gtgtccgagg tgtccgcctt cgccggcgtg gggattaagt ccggcgacaa ggattttggg
      481 atttttgatg ccgaatttgt gttttaagtc gtgatgcaaa atgcgtgcga cgctttcaat
      541 cagcggttgc gtgatggcgg cggcaacgtc tttcagcggc aggtgggtcg ttacgagggc
      601 gacgcgcagg cctttgccgg caagcatcat cacgacctgc cccgtgccgc ttttttccgc
      661 cagatattcg gtgtgtccgc tgaaaaaacc tgtgcttgcg cgcgcgtcgt tgatgatgcc
      721 tttgtgcagc ggcgcggtaa cgatgccgtc gaaaatgccg tctgaaatgc ctgcgagcgc
      781 ggtgtccaaa agttgcagca cataggcggc gttggcggga ttgagtttgc ccgcctcaac
      841 cgcttcgacg gcagggatgt gcagcacttc cagctcgccg tatgccgcgc cgcctgattc
      901 tggatcgaag tcgcgcagga cgacgctttt gcccaaggct tcggcgcgcg cgcgcaatag
      961 gtttttgtcg cccaataccg cgcagcggca gggcaggcgt gcaaacgcca agtccaaaca
     1021 aatatcgggg ccgatgccgg caggctcgcc ggaagtaacg gcaaaaacag gctgtttcat
     1081 cgtgtttgct ccaaacaaaa tgcgattcta acgccgcagc cgcgcggcga tgtaaatttt
     1141 tctgattttg ttgacaatct gctagaatgg gcgtttacaa aatttaaacc ctgcttgcat
     1201 accgccaata tgtgcgagtt tcaactttaa ggaagcgata tgaacgagaa ctttaccgaa
     1261 tggctgcacg gctgggtcgg cgccatcaac gatccgatgt ggtcatactt ggtttatatg
     1321 cttttgggta cggggctttt cttcaccgta accacgggct ttgtccaatt ccgcctgttc
     1381 gggcgcagca tcaaagaaat gctcggcggc cgcaaacagg gggacgaccc tcacggcatc
     1441 acgccgtttc aggcatttgt aaccggcctt gccagccgcg tgggcgtggg caatatcgcg
     1501 ggcgtggcca tcgccatcaa agtcggcgga ccgggcgcgg tgttttggat gtgggtaacc
     1561 gccttaatcg gtatgagttc ggcgtttgtc gaatcttcgc tggcgcagct ctttaaagtc
     1621 cgcgactacg acaaccacca tttccggggc ggccctgcct actacatcac tcaagggctg
     1681 gggcagaaat ggctgggcgt gttgttcgcc ctgagcctga ttttctgttt cggctttgtg
     1741 tttgaagcgg ttcagaccaa taccattgcc gataccgtca aagcggcgtg gggttgggag
     1801 cctcattatg tcggcgtcgc cctggtgatt ttaaccgcgc cgattatctt cggcggcatc
     1861 aggcgcatat ctaaagcggc ggaaatcgtc gtccccctga tggcggtttt gtacctcttt
     1921 atcgcgcttt tcatcatttt gaccaatatt ccgatgattc cggacgtgtt cggtcagatt
     1981 ttttcgggcg cgttcaaatt cgacgcggca gcaggcggct tactcggcgg tctgatttcg
     2041 caaacgatga tgatgggcat caaacgcggc ctgtattcca acgaggcggg tatgggtt
//
LOCUS       DAXHPG010000224         2069 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXHPG010000224 DAXHPG010000000
VERSION     DAXHPG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.224"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(383..793)
                     /locus_tag="ACFM6F_002058"
     CDS             complement(383..793)
                     /locus_tag="ACFM6F_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165404.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(1381..1764)
                     /locus_tag="ACFM6F_002059"
     CDS             complement(1381..1764)
                     /locus_tag="ACFM6F_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165405.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(1730..>2069)
                     /locus_tag="ACFM6F_002060"
     CDS             complement(1730..>2069)
                     /locus_tag="ACFM6F_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC6165406.1"
                     /translation="PPKPSTQQHSTHSDNNIGLPAPYVKPDTSISPTGTIYVDSKGNA
                     IPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHHANTHQDPRALTGHAHVPGVTN
                     DDGTPWLPIK"
BASE COUNT          614 a          360 c          344 g          751 t
ORIGIN      
        1 ctctacggat ggggcagagt tacccgtatg attgccgtat ggtcggatac ttgtatccga
       61 caaaaatatt tgacgcgtct atcgtttccg aaatgccgct gttgaaaaat gtcggattcg
      121 agaatccgac ctacggttgc tgaacaatgt tttgtagaac ttgctcaaaa tccatgtttc
      181 gccattttcc tttatatata cctaatacag cacgggttac gatttcgttt aagtcataaa
      241 tagccattta cgtcctctgc taattttcag cgagtagatt gggttaccaa acccaacaaa
      301 aaagcagcct gaaaaatttc aggatgcttg ttttatgttt tttatcaatg aattaggttt
      361 taatccagcc tgcgcttgct agttaaacag tatttacata aaaatcttgt actgaacaaa
      421 caagcgtatc atgttcatca tgaaatgatg ggcataacgt taatctacaa atgtttacat
      481 ttaaagatat tttttcagtt tcaatatttt taatttgtaa ggttaataca tgaggatatt
      541 tgtgatatag ctgatttttt aattgttcgt tgtctaatat agcaaaaggt aattttgcac
      601 acaaataacc aataactttg tttttatgaa aaaaaactat ccaattttca attccttcat
      661 cccaatcaaa agtaaaatcc gttccttcta tgataaattt agcacaagat attaaatcta
      721 acttaaccca agtggattta tacagactca atgctgatga agttataata ttatctaaat
      781 tataaatttt cataaatcaa cgttccttac ctggtgttat atgcacaggt ctaatcccat
      841 ctttattcgg ctttctacga taaatgttaa ttatatgtgt cggttttccg tctgtatagc
      901 caactacgtc gttcgttgga acttttgcct tattttgact atcccataaa ttatgtttat
      961 ctgcatatct agcagcatct aatgtagctt tttccgcatc aacatctgat ttaaataaac
     1021 ttttgctatc tatcttgtct agactagatg catcaggttt gtgtcgtaaa aagcactgtc
     1081 ctgtaatagg ctcgcggcag caagttgtgc ttccggactt aattttccta aatcattaat
     1141 accttgtaaa gtttgctgcg cagagatgta ggtcggatat ttgtatccga caaaaatatt
     1201 tgacgcatct atcgtttccg aaatgccgct gttggaaaat gtcggattcg agaatctgac
     1261 ctacggctgc tgactatttt cttgtttata taattctaca actgcaaact actatcaaat
     1321 ctttttatct ctggctctac actcagcaag aatattttta ttttacaaaa cataggctag
     1381 ttatttgagt atattgtcaa attctaattt gtttttttga tagatttttt ttataccatt
     1441 aatacattca ttagttgtaa ttctaccaag cgaatgtaat ttatcaatat caaaacaatc
     1501 ggtataaacc ctattcacag ttttatcttg tatttcctta tgaatactta atatattgag
     1561 atagtttaga aattttattt tctcagattg aggtaactca tcaaaaccaa gccaataata
     1621 aaaattgtta tatgatttta aaaaatcaac aatttttatt tttcctaaaa ccaaatcttc
     1681 aactaactgg gttgcataaa taatatcatt tatgatttta acaggtaaat tatttgatgg
     1741 gtaaccatgg ggttccgtca tcatttgtaa ctcctggtac atgtgcgtgt cctgtaagtg
     1801 cgcgtgggtc ttgatgggta tttgcatgat gagcatcatc tattctggct ccagtcaagt
     1861 ttccattact atctcggact tggataaaac gagcatcagg cgaacctgtt atataaccgc
     1921 ctattggtgt tggtattgca tttcctttgc tgtctacata aattgttcct gtcggagaaa
     1981 tagatgtatc aggtttaaca tatggggcag gtaagcctat attgttatca gagtgtgtag
     2041 agtgttgctg cgtacttggt ttcggtggc
//
LOCUS       DAXHPG010000225         2006 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXHPG010000225 DAXHPG010000000
VERSION     DAXHPG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.225"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            301..1758
                     /locus_tag="ACFM6F_002061"
     CDS             301..1758
                     /locus_tag="ACFM6F_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6165407.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGATTGFLLLLLVPKLNKMLEGTD"
BASE COUNT          451 a          601 c          463 g          491 t
ORIGIN      
        1 atagtacggc aaggcgaggc aacgccgtac tggtttttgt taatccacta taaaaaaccg
       61 ccctatcgga taagccctta acagaaaagg ctttacccgc gccgtatcgg aacacatcct
      121 ctaaaataca atccgttgaa ttgaaaaaaa tataaaaaca tccgcccgcg aaaaacggca
      181 gcgcgtcgtt tgacaaagaa tgaaaatatc ggttaaaaac cgattttcat acaaaaaaca
      241 ccgctgccgt ccgcatccgt ttcagacggt attgagagaa aatcttttag gagaaccttt
      301 atgtcccggc atcccgcccc caccggagaa aaaacattct tcggccaccc cttccagctt
      361 tccaccctct tccatatcga attgtgggaa cgtttttcat tttacggaat gcagggcatc
      421 ctgctgattt acctctacta caccgccgac aaaggcggct tgggcataga caaaaccctc
      481 gccggcggca ttgtcggcgc atacagcggc agcgtgtacc tgtccaccat tttgggggcg
      541 tggtttgccg accgagtatg gggtgcggaa aaaaccctct tcctctcggg catcgtcgtg
      601 atgctcggac acatcgtcct tgccgccgcc ccgggcctgt acggtctttt aatcgggctg
      661 atattcatcg cattgggcag cggcggtgtg aaatctacgg ccagttctat ggtgggcgca
      721 ttatacgaac aggacgaaat gcgcccgctg cgcgatgcgg gattttccat tttctacatc
      781 gccatcaaca tcggcggctt cctgggcccg ctgctgaccg gcctgctgca ggaaaacatc
      841 ggtttccatt atggtttcgg cgcggcggcg gtcggtatgg cattcggctt gtggcgttat
      901 tccttgggac gtaaaaacct gccccacccc accgtccccc atccgctttc aaaaggacag
      961 ggcaaaactg cggccgccgt cggcatcacc ctcatcgccg cacttgcaac cgccatcaaa
     1021 accgggcttg tcaacctcga caatttctcc ggcatcctct tatctaccgt catccttgcc
     1081 gtcattgcct atttcgcccg cctgctgacc aacccccgcg tcagttccga caacaaacgg
     1141 cacatcatcg cctacatccc gcttttcctg accatctgta tgttttgggc cgtctggttt
     1201 cagatttaca ccgtggcaac cgtctatttc gacgaaaccg tcaaccgcac catcggttcg
     1261 tttaccgtgc ccgtcgcctg gaaagattcc atgcaaagcc tgtgggtcat cctgttttcc
     1321 ggactgatgg cggcaatgtg gacaaaaatg gggcgcaaac agcccaaaac cccgctgaaa
     1381 ttcgctatgg cggtatttgt taccggcgcg tcgtttttgg gattcgtccc ctttatttcc
     1441 gccggcacgc cgatgcctat tgcggttttc gcactgattg tccttgccat cacgataggc
     1501 gaactgatga tttccccgat tgcgctgtcc atctccacca aaatcgcacc gcctttattc
     1561 aaaacccaaa tggtcgccct taatttcctt gccttttcat taggcttcac tttgggcggc
     1621 gtattgtttg agaaaggcta tcaggcgggc gacgaaatcg gcttctaccg gctgctgttc
     1681 tacatcggcg caaccacagg cttcctgctg ctcctgctcg tccccaaatt gaacaaaatg
     1741 ctcgaaggca cagactaagt cccgccccga tgcgccgatg ccgtctgaac ccttcagacg
     1801 gcatttttcc gcataatgaa accaaaccgt ttccacccga caggacaggc tccccccaac
     1861 cggaaggcag cctgccgatt gtcatttgaa tatctgactg attccgtctg tttgcaaggg
     1921 aaatagccgt ttatttccgc ttgggcggaa accggttcta ttgagtaaaa ggcattttat
     1981 ccgactaaat tagtgctgca tcaacg
//
LOCUS       DAXHPG010000226         1968 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXHPG010000226 DAXHPG010000000
VERSION     DAXHPG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.226"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            208..1287
                     /gene="apbC"
                     /locus_tag="ACFM6F_002062"
     CDS             208..1287
                     /gene="apbC"
                     /locus_tag="ACFM6F_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC6165408.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIAKIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
BASE COUNT          487 a          609 c          500 g          372 t
ORIGIN      
        1 aagccgtgca ttatacagca ccatcctacc tcgtcaagca aattttacag gcttaattgc
       61 agaccactgt ttgcacggga tattttgaca acggattttc acaatccgcc gcataccgtg
      121 taaaagttcg cacaaggaaa agcaaaccgc ccgaaatcaa tgtacacttt ccgcccgttt
      181 cccaaactgc acacagaaac acacattatg aacatacaaa acatccgcac cctcctcgac
      241 accgtcgccg ttccgaatac ggcacgcacg ctcggcggcg aaaaggccgt ccgttcggtc
      301 gaacagcgtt cagacggcat ccatatcgcc ctgcatttcg gtttccctgt cgcgcacatt
      361 gcctcagaaa cagccgaccg catacaggaa atcctgatgc cgctgacagg cgacacacac
      421 atccatctgt ccatggacac tgaaatcggc acacataaag tccaacctgg cgttaccacc
      481 atcaaaggtg tgaaaaacat cattgccgtc gcctccggca aaggcggcgt gggcaaatca
      541 acaaccaccg ccaaccttgc cgccgcaatg gcgcggatgg gcgcgcgcgt cggcgtgctc
      601 gatgccgacc tttacggccc gagccaaccg accatgttgg gtgtggacga ccgcaaaccc
      661 gatcagaaaa accaaaaact cattcccgtc gaatcttcag acggcataca ggtcatgtct
      721 atcggctttc tcgtcgatac cgaccaagcc gtcgtctggc gcgggccgat ggtcagccaa
      781 gccttgcagc aactgatgtt tcaaagcgag tgggacgaag tggactacct gtttatcgac
      841 ctgccccccg gcacgggcga catccaactc acgctgtccc aacgcatccc cgtaaccgga
      901 tcggtcatcg taaccacgcc gcaggacatc gccctgatag acgcgcgcaa agccgtggat
      961 atgttccgca aagtcaacat tcccattttg ggcgtattgg aaaatatgtc cgtccacatc
     1021 tgctccaact gcggacacag cgaagcactg ttcggcacgg acggcggcaa agatttggcg
     1081 gcacgcctca acgtccccct gctcggacag cttcccctaa gcctgcccgt gcgcgaagcc
     1141 atggacggcg gcacaccggc gcaactgttc gacgaacacc ccgccatcgc caaaatctac
     1201 accgatgccg cattccaaat cgccctgagc attgccgaca aaggcaaaga cttcagcagc
     1261 cgcttcccta aaattgtcgt cgaataaagc cgcgtccgaa accgcaacag caatgccgtc
     1321 ccaagccccg cgcctgccgg cgggcttcat agtcctctct ccccgtggga gagggctgga
     1381 gagagggcaa caagccgcaa ggcttgtatt ggggcggtta agctgttggg aaagattgcc
     1441 gcaacttgga gaatgccctc tccccggccc tcccccacag gggagggagc ggattgcggc
     1501 aggttttgcg gttgcaggcg gtttgaaaaa cagattggat ttacaaccgt tgatgcaggt
     1561 cgtctgaaaa gcaaaaagca gcctgcacaa cctgattagt ttacaaaacc cttaacccca
     1621 acagcctccc cgtcctctct ccccgtggga gagggctgga gagagggcaa caagccgaaa
     1681 ggcttgtatt ggggcggttg agttgttggg aaaggttgcc gcaacttgga aaatgccgcc
     1741 tccccggccc tcccccacgg gggagggagt gggtttcggc agatttttcg gttgcaggcg
     1801 atttgaaagg caacttagat ttgcagctgt tgtttcaggt cgtctgaaaa ataaaaagca
     1861 gcctgcacaa cctgttttcc ttgcagcaaa cctcaacccc aacagccgac ccgtcctctc
     1921 tccctgtggg agagagctag agagagggca acaagccgaa aggcttgt
//
LOCUS       DAXHPG010000227         1929 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXHPG010000227 DAXHPG010000000
VERSION     DAXHPG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.227"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(26..349)
                     /locus_tag="ACFM6F_002063"
     CDS             complement(26..349)
                     /locus_tag="ACFM6F_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6165409.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(368..577)
                     /locus_tag="ACFM6F_002064"
     CDS             complement(368..577)
                     /locus_tag="ACFM6F_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HFC6165410.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFFRN"
     gene            complement(700..1497)
                     /gene="truA"
                     /locus_tag="ACFM6F_002065"
     CDS             complement(700..1497)
                     /gene="truA"
                     /locus_tag="ACFM6F_002065"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC6165411.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQTALETALAQI
                     AGEFVATTVAGRTDTGVHATAQVVHFDTTAVRPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPKIPEWL"
BASE COUNT          435 a          456 c          542 g          496 t
ORIGIN      
        1 aacacaatcc attcagacta ttcaatcagg caaacatctc ctgcaatact gcaaacagtt
       61 tttcagccgt actgttgtct aaattgccaa gatgcttgac caatccggct ttatccacag
      121 ccctaatctg ttcgggcaaa agcaaaccgt ctttatcctg aaagcggaca ttgacgcgga
      181 acggggcagg acggcttccg ctcgtcatgg gaacgatcag cacagtcttg agatagttgt
      241 gtatttcagg aggagagact acgacacaag gacgtgtctt tttgatttcg cttcctacgg
      301 tcgggtctaa ggagaccaga tagattccgc cgcgtactac catatccatt ctttatccgc
      361 ttcgttttca atttcggaaa aaaaaatgct cctgctcggt ttcgacaagc attgcggcag
      421 cttctgccca tcccctgcga acggtaggac agcttaaaat aatattgccc ttttcaactg
      481 taacagccaa gctgtctact gcccctattt gacccaataa tgatttgggc agaatcacgc
      541 cttgcgagtt tcccattttg cgtatgttga gaatcatata cgtaccctat ttaccagttt
      601 atgtaataac aatgttatta ccttgatgag gtagtgtcaa cattgaaaaa gattgcctga
      661 cagtttgtcc gatttcaaaa tctccgcgac aagaatgttt taaagccatt cggggatttt
      721 ggggcggatg atgccgtatg cctcgggata gtcgacgccg gtcaggtaaa gcccgtcggg
      781 catgaaggtt ggtggggctt tgaggcggct gcgttcttga atcagtgcgg cgaagccttc
      841 gacgctgagc ctgccgctgc cgacataaac gagcgcgccc atgatgttgc gtaccatatg
      901 gtgcaaaaag gcgttgccgt gcaaatcgag gcggacgagt cctgcgcttt gggtaaggtc
      961 ggcgcggtag atggttttga cgggggattt tgcctggcac tcggcggcgc ggaagctgga
     1021 gaagtcttgt tcgccgatca aataggcggc agcccgacgc atcggttcga tgtcgagttt
     1081 gaggtgtgtc cagcctgccc tgtttttgag caggggggaa cggacggggg cggattcgag
     1141 caggtagcgg tagtgtcgtc cgtatgcgtc aaatcgtgca tgaaattcgg gggcgacctg
     1201 tcgagcatgc aaaacggcaa ttccttcggg caggtgggca tttacgccgc gcacccatgc
     1261 ctgttgggga cggacggcag ttgtgtcaaa gtggacgact tgggcggtgg cgtgcacgcc
     1321 ggtatcggtt ctgccggcaa cggtggtggc gacaaattct cctgcgattt gggcgagtgc
     1381 ggtttccaat gccgtctgaa cggtcggtac gccgcccgcc tgtttctgcc agccgtaaaa
     1441 gcggctgccg tcataggata gggttattgc ccagcgttgt ttttgtgcgg tatccatcgg
     1501 atttgggatt cggataaatg ttcagactgc attgtatcgc agattttgca gggaaacggc
     1561 aaacgcccag ggcgagcggc gttgtttggg gagttggggg ggggatgcag ttgctacgaa
     1621 tcgctatcct gtgaattcac cctgtcagga gtgcccgaat cgtcattccc gcgcagtcgg
     1681 gaatctagga cgtaaaatct aaagaaacca ttttatagtg gattaacaaa aaccagtacg
     1741 gcgttgcctc gccttagctc aaagagaatg attctctaag gtgctgaagc acgagtgaat
     1801 cggttccgta ttatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
     1861 cactatatcc gataagtttc cgcaccgaca aaactagatt cccactttcg tgggaatgac
     1921 gggatgcag
//
LOCUS       DAXHPG010000228         1918 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXHPG010000228 DAXHPG010000000
VERSION     DAXHPG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.228"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            275..1747
                     /gene="pyk"
                     /locus_tag="ACFM6F_002066"
     CDS             275..1747
                     /gene="pyk"
                     /locus_tag="ACFM6F_002066"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC6165412.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVTAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          498 a          549 c          510 g          361 t
ORIGIN      
        1 cgactttttc tcatagggat aattctaact taatttgaat ttccctagtt atctaggaca
       61 gctccaaaaa taaatttagc ctaagtcata aaaccgtgta attttgcacc aaaaaagacg
      121 gtttgcaggt aaaatatcaa actattaatt taaatcatat ttttacagaa tattccgccg
      181 cattcatcaa atggacatca aaccggttcc aaatttctgt aattttgcaa caaaataccg
      241 caaaacaccc gattgagacc gaaaggactt tcatatgaac cagacaagcc gcgatctgac
      301 ccgcatcagc cacaacacca aaatcgtcgc caccttgggg ccgggcagca acaacgtcga
      361 actgttggaa gacatgatcc gcgtcggcgg tctgaacgtc gtccgcttca acttcagcca
      421 cggcacgccc gaattccatc aggaaaacgc ccgcattgtg cgcgaggcgg caaaacgcgc
      481 cggacaggaa atcgccatca ttgccgacct gcaagggccg aaaatccgcg tgggcaaaat
      541 cgccggcggc ggcatcgaat tgaacaaagg cgaaacgctg gtactggatg ccgcgctcga
      601 aggcgaaggc acgcgcgagg cggtcggttt ggactaccgc gacctgcccg acgacgttac
      661 cgcaggcgac gtattgtggc tggacgacgg cctgctgacc ctgaccgtgg aatccgtcga
      721 aggcagcagg attatcacaa gggtggaaaa cagccatgtc ctgaaaagca acaaaggcat
      781 caacaaacgc ggcggcgggc tgtccgcagg cgcgttgacc gaaaaagact tccgcgacct
      841 gaaaaccgcg attgccatcg gttgcgacta cctcgccatc agctttgtga aatctgccga
      901 agatttgcac atcgcgcgcg ccaaagtcga agaagaaatg aagggcagca ctgccgtgcg
      961 ccccggtttg gtttccaaaa tcgaacgcgt agaagcgatt gaaaaccttg atgaaatcat
     1021 cctcgccggc gacggcatta tggttgcgcg cggcgacttg gcggtcgaag tcggacacgc
     1081 cgccgtcccc gccctgcaaa aacggatgat ccgccgcgcc cgcgagttgc gccgcttcag
     1141 cattacggcg acgcaaatga tggaatcgat gattaccaac cccgtgccga cccgcgcgga
     1201 agtcagcgat gtggcaaacg cggtattgga cggtaccgat gcggtgatgt gttccgccga
     1261 aaccgccgtc ggcgcgtatc cgtttgaaac cgtcagccaa atggcaatta tctgcgcggc
     1321 tgcggaaaaa gagcaggatt cgctcaacgg cgttgccgaa caggcggagt atcccgaagc
     1381 ggtcagcacc aacctggcgg ttgccggcgg cgcggtcagc gtggcgcgcg cggttcacgc
     1441 caaagcgatt gtcgccctga ccgaaagcgg ttcgaccgcc tttgaaatca gccgccacaa
     1501 catcaccctg ccgattttcg cgctgacccc gagcgtttcc gcccaacgcc gtatggcgat
     1561 gtaccgcggc gtgcgcccgc tgattttggc aaccagtacc gaccacgaca cggcgttgaa
     1621 cgaagtcgaa accatgctgg tggaacacaa catcctgcat tccggcgacc aatacatcat
     1681 caccagcggc tcgcaaatgc gcgaatccgg ttcgaccaat acgctggaag tgctgcgcgt
     1741 caaataatcc gccctgagtg gaaaatgccg tctgaagtcg atgcccgagg cttcagacgg
     1801 cattttttgc ggcggcgcgg cggttcaggc aaactgaaaa aattgtgcaa atccggcaac
     1861 atcagataaa attgagtacc tatactaaag cgaaacaagg catttccgac tgcctttt
//
LOCUS       DAXHPG010000229         1883 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXHPG010000229 DAXHPG010000000
VERSION     DAXHPG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.229"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(127..1437)
                     /locus_tag="ACFM6F_002067"
     CDS             complement(127..1437)
                     /locus_tag="ACFM6F_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC6165413.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKSLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPYVENGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLGAAETR
                     RLKTLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRALQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDCLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            1692..1883
                     /locus_tag="ACFM6F_002068"
                     /pseudo
     CDS             1692..1883
                     /locus_tag="ACFM6F_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213469.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          454 a          494 c          480 g          455 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagca
      241 gtcgggcata tagctttcgc cggcggcgta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcatcag cttggtcggg gcattgcgca ggtggacggg
      361 tacttcgtcg ctggcgtttt ctttgacgaa acggcgcatt tgattgtatg ccttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaagata caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt gcaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcagtca gggttttcag
      781 acggcgcgtt tcagcggcgc ctaacagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaaggattg
      961 caaaacatac acctgcgcgc ggctcaacag cgcgggattc acttcaaacg acggattttc
     1021 cgtcgttgcg ccaataaagg tcagcaaacc gttttccaca taaggcaaaa acgcatcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgccc tcccctgctg
     1141 caaagcgatt tcagccttgt cgattgcccc gcgtatgtct tttacgccgg aaaacacggc
     1201 ggaaacaggc agaaactggg cattgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccccctt ctaccgccac
     1321 gcgcaaagat ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcaccggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gattcaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aaaagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 tgaaaacttt agaaaattag aac
//
LOCUS       DAXHPG010000230         1867 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXHPG010000230 DAXHPG010000000
VERSION     DAXHPG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.230"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..137)
                     /locus_tag="ACFM6F_002069"
     CDS             complement(<1..137)
                     /locus_tag="ACFM6F_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6165414.1"
                     /translation="MSTFFQQTSQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YL"
     gene            193..606
                     /locus_tag="ACFM6F_002070"
                     /pseudo
     CDS             193..606
                     /locus_tag="ACFM6F_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fe(3+)-pyochelin receptor"
     gene            749..1807
                     /gene="dinB"
                     /locus_tag="ACFM6F_002071"
     CDS             749..1807
                     /gene="dinB"
                     /locus_tag="ACFM6F_002071"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC6165415.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCASLLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT          439 a          495 c          516 g          417 t
ORIGIN      
        1 aggtaacggg ttttttgacg gttcaggtat tgttcgattg gctgccaatc aatcacttga
       61 tccaacttca atagagggaa gcggtcgatg tgtttggcaa tcatggcttg ggaggtttgc
      121 tggaagaagg tgctcatgga aaatccccta aatggcttgg tgggaattta ggggattttg
      181 gggaattttg caaaggtctc aggttatacc tacaacaaga gcaggtatag gaatgcagcc
      241 gaagtcaacg ccgaacgcct tgccaaaaat tccagtgcag acccgtacaa cttcagcaat
      301 ttcacacccg tgcatatatt ccgtttcgga acgagtttcc atataccgaa tacggggctg
      361 accgtcggcg gcggcgtgtc cgcacaaagc agcacaagca gcctgtacaa catcaggcag
      421 ggcggctacg ggctgataga cggtttcgtc cgttacgaat tgggcaaaca cgccaaattg
      481 agcctcatcg gtacgaactt aaacggacgc acttattttg agaacaacta caaccgtacg
      541 cgcggcgcaa acaacttcta tggagagccg cgcactgtca gcatgaaact ggattggcag
      601 ttttaattga aaaccgtagt tttgcggatg ccgtctgaac agggtatgtt tcagacggca
      661 tttttatgga ggcaggccta tcggggcgta tatttggaat ttgctcgatg ccggcagtag
      721 aatatccttt ttatcttcag cagagccgat gtcttcacgc aaaatcatcc acatcgacat
      781 ggacgcgttc tacgcatcgg tagagctgcg cgaacagccg catttgaaag ggcggccggt
      841 ggtcgtcgcg tgggagggcg cgcgttcggt gatttgcgcc gcatcgtatg aggcacggca
      901 gttcgggctg cattccgcga tgtcggtggc aacggcgaaa aggctgtgtc cgcaagcggt
      961 gtatgtgccg ccgcatttcg atttgtaccg tcaggtttcc gcgcagattc acgccgtatt
     1021 caggcgttat accgatttaa tcgaaccctt gtcgctggac gaagcctatc ttgacgttac
     1081 ccgtaatttc aaaaacatcc cttacgccag tgaagttgcc aaagaaatcc gtgccgccat
     1141 ttttgcggaa acaggtttga ctgcatccgc aggcatcgcg ccgaacaaat ttctggcgaa
     1201 aatcgcgtcg gactggcgca agccgaacgg gcagtttgtg ttgccgccgc acaaagtcat
     1261 ggcatttttg gaaaccctgc ctttgggcaa aatccccggc gtgggcaagg taacgctgaa
     1321 aaaaatgcag tcgctgggta tgcggacggc gggcgacttg cgccgtttcg agcgcggcga
     1381 gctcttaaac catttcggac gctacggcta ccgcctctat gatttggcgc gcggtacgga
     1441 cgaacgcccc gtcaaagccg aacgcgaacg cctccaaatc tccacagaaa ttaccctgcc
     1501 cgaagacctg ccgctcgagc aggctgccgg acacctcccc catcttgccg aagacttgtg
     1561 gcggcaaatc acgcgcaaaa acgtcgaagc ccaaagtgta acgctcaagc tgaagaccta
     1621 cgatttccgc atcatcacgc gcacgctgac ttattcctcc gtattgcccg actgcgcctc
     1681 cctgcttcag gcggcgcaaa tgttgatggc gcgcgtcccg ccgcagacgg aagacgcgtt
     1741 ccgccttatc ggtatcggcg tggggcatct tgtgccgaaa aaccagcagc aggatttgtg
     1801 ggcgtaaacc gctttacgcg cgccgtccaa aaaatatgcc gtctgaacgg ttttcagacg
     1861 gcatggc
//
LOCUS       DAXHPG010000231         1852 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXHPG010000231 DAXHPG010000000
VERSION     DAXHPG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.231"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..202)
                     /locus_tag="ACFM6F_002072"
     CDS             complement(<1..202)
                     /locus_tag="ACFM6F_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165416.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNR"
     gene            complement(199..744)
                     /locus_tag="ACFM6F_002073"
     CDS             complement(199..744)
                     /locus_tag="ACFM6F_002073"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6165417.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMPLRDAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            complement(901..1329)
                     /locus_tag="ACFM6F_002074"
     CDS             complement(901..1329)
                     /locus_tag="ACFM6F_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFC6165418.1"
                     /translation="MAFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(1310..1744)
                     /locus_tag="ACFM6F_002075"
     CDS             complement(1310..1744)
                     /locus_tag="ACFM6F_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFC6165419.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          449 a          534 c          381 g          488 t
ORIGIN      
        1 gacgattctt gcgtttgcgc gccgcgcgtt tggctgccgc cacgcctgag tttcttaaat
       61 ttgggcgtgg ataggtgtgt atcggacgaa catttggcat ctgtataagt gcgcggttta
      121 cctgctcatt actaggattt tctccaatca attttgtaag aaaataggaa gcagcttctt
      181 tcaatttacc aataatattc atactcttgc cgcccccaat tccatcgcaa tcgcgtccgc
      241 aatcgcgcgg cagatgcccc atttagtagt cttaaacaag gctaaatcag tgtcgttgct
      301 gataaaaaac ggctcaaaca caatgccgcc ggcttgtgca taagccaggc gcgaatgctg
      361 gcccgcattg tcgggtttaa agccgtcttc gccgcgcagt ttccagccgg ttttcttggc
      421 aacagctttg ctcaacacct gacaccagcg tttgttttta acggtactca aggcttcgat
      481 gcctgtcgcc gctttgctga cggcagcgtt ggtgtgaaac tcaatcgcca catccgagcc
      541 gcgaatcagc ttgaccgcat cgcgcaaggg catattgcct ttgccttcgc cgtcggtgcg
      601 tacggtcagg ccgtaatcgt tacgcaggat tgaagccacg atgttgcgca tatcctgcgc
      661 caagtccgcc tcacggtcgc ttccgttgac cgcgcccggg tcggtgttac tgtgtccggc
      721 ggttaaagtt acagttttgc ccattcatca tctccaaaaa atattgattg cataacattt
      781 gaaaacccca ttaaaccgtc tttaacccat cgtcccgaac ggtagatgtt tcatccccta
      841 caaaccaaaa aaaaagaccg tctgaataca gacggccaaa gcctggtcac acatcactgc
      901 ctaaaataaa gctgcctgct gtgccgcagg tcggctcatt tcattgataa tcgtatatcc
      961 tgttcgtgaa gagataccgt atttagggca tagcttcgtc atcgccataa gcccgctctc
     1021 cttatcaata tcgcgcaatt tgacaaactc ctgataaaac ctatggtttc tcaactgtat
     1081 taacgccttg ccgcaccgtg ggacatacaa ttcctcgcca ccatatacct gcaacagctc
     1141 atgtgttttc acttcgccga tggcttcgac caaaattgcc aaacgctcgg tgtccacctt
     1201 gcccttacca aatttaaacc gcgccccgcc aatcgccttg accagctgtt ccgtcgctgc
     1261 cagtccgatg acatccacaa tgtccaacac ggtatccggc aataaatgtt caactttttc
     1321 gaacgccatc atccccaccc gctttttccg ttttcctgtt ttccgcaatc tgcaacgcag
     1381 caaccagttt atgtagctgc gtatcgtcta aatattcgac cttatcctta ccaaacatcc
     1441 gccgcgccat tgcgtgtgca tagttccaat gtttgccgcc gacggtcagc agggcttcga
     1501 ctttgtccaa cattgccgct gatgatgtcc gacgcagatg cggtttgccg tgtgggttac
     1561 cttttgcttt aggcttaaat ccgtgcgacc gcatatcagc gacaacagac tcaagttcgg
     1621 aaacatccat atccgcacac gaccgcttgc ccgtcacacg ctccaacacc gcgcgatagg
     1681 taccgtcgtc caagcccagc tccttttgag caatcttaat tttcgcaatc aacgcccggc
     1741 gcatctcaaa cccataaaac acaatatatt gactaattaa cgcatattat accaataaaa
     1801 tacaatatat agtattaagc cgatgttttt tgcgaaacgg acagacataa aa
//
LOCUS       DAXHPG010000232         1835 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXHPG010000232 DAXHPG010000000
VERSION     DAXHPG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.232"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..243
                     /locus_tag="ACFM6F_002076"
     CDS             <1..243
                     /locus_tag="ACFM6F_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6165420.1"
                     /translation="LVLIFVNPLHMELETVVPPEEGEGEGEGGDGEIPEYLELHSERE
                     DDISAPSQAEPPPRNIKPLVFGLLIAFLGYLLGKIF"
     gene            278..1789
                     /gene="ubiB"
                     /locus_tag="ACFM6F_002077"
     CDS             278..1789
                     /gene="ubiB"
                     /locus_tag="ACFM6F_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC6165421.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAVWVERLFADGKRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT          405 a          495 c          496 g          439 t
ORIGIN      
        1 cttgtcctga tttttgttaa tccactacat atggagttag aaacggtcgt gccgccggag
       61 gaaggggagg gcgaaggaga aggcggggat ggggaaatac ccgaatatct tgaacttcat
      121 agcgaacggg aagatgatat ttccgcacct tcgcaagccg aaccgccgcc ccgcaatatc
      181 aaaccgctgg ttttcgggct gctgatagcc tttttgggct atctgctcgg taagattttt
      241 tgattgtccg ataaatgctg tattcgggat tttatatatg aaatggttga aacgcctgac
      301 ggttattgtc gggacttttt accgctatcg gctggcaggt ctgtgcgctt cgctgatggg
      361 tagcggttgg atatgcgctc tgctgaaaat gatgccgcag tcgtccaaat tgaaaaacga
      421 accgcctgcc gtccgtctgc gccttgcctt ggaaagcctg gggccgattt tcatcaagtt
      481 cgggcaggtt ttgtccacac gccctgattt gattccgcac gattacgccg tcgaactggc
      541 aaagctgcaa gacaaagtgc cgccttttga cgcgcggctt tcgcgtgaac aaatcgaaaa
      601 atcgttgggt cagtccatcg aaaagctgta tgcggaattt gaaaccgagc ccgtcgccag
      661 cgcgtccatc gcccaagtac acaaagcccg cctgcattcg ggcgaacgag tggctgtcaa
      721 agttttgcgc cccaaccttt tgccccttat cgaacaggat ttgtcgctga tgcgtttcgg
      781 cgcggtttgg gtggagcgtc tgtttgccga cggcaagcgt ctgaagccgc tcgaagtggt
      841 ggcggagttc gacaaatacc tgcacgacga gttggatttg atgcgcgaag ccgccaatgc
      901 cggacagctc gggcgcaatt tccaaaacag caatatgctg attgtgccca aggtgtttta
      961 cgactactgc accagcgacg tgctgacaat cgaatggatg gacggcacgc cggttgccga
     1021 catcgccaaa ctcaaagcag acggcatcga tttgcacaaa ctcgccgatt acggcgtgga
     1081 aatctttttc acgcaggtct tccgcgacgg ctttttccac gcggatatgc accccggcaa
     1141 tattttggtt gccgccgaca accgctacat cgccctcgat ttcggcatcg tcggcacgct
     1201 gaccgattac gacaaacgtt atctcgccat caatttcctc gcctttttca accgcgatta
     1261 ccggcgcgtc gccaccgccc acatcgaatc gggctgggtg cctgccgaca cgcgtgcgga
     1321 agaattggaa gccgccgtcc gcgccgtgtg cgaaccggtg ttcaacaaac cgatttcgca
     1381 gatttccttc ggcttggtgc tgatgcgcct gtttgaagtc agccgccgct tcaatgtcga
     1441 aatccaaccg cagcttgtgc tgctgcaaaa aacgctgctc aacatcgaag gcttgggccg
     1501 ccagctcgat cccgatttgg acttgtggaa aaccgccaaa ccgtttttgg tgaaatggat
     1561 gaacgggcag gtcggcccta aagccctttg gcgcaacctc aaaaacgaag cccccgactg
     1621 ggcgcaaatc atcccttcat tgccgcgcaa aatcagtgcg ttgattgatg aaaaccgcca
     1681 gcaggaaatg cgtgatgcct atatccattt ggtcaaagtg cagcagcggc aaagcctgtg
     1741 gctggctgtg attgcggttg ttttgctgct gattttgctt ttgaaatagg ctttgtccga
     1801 atcatcgccc gactccgccc gtttataagg aaatc
//
LOCUS       DAXHPG010000233         1825 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXHPG010000233 DAXHPG010000000
VERSION     DAXHPG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.233"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..1717
                     /locus_tag="ACFM6F_002078"
     CDS             <1..1717
                     /locus_tag="ACFM6F_002078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165422.1"
                     /translation="NEHFEQDLIPYAVRFRLNQILPELDCHWQENLTEIFKTLSPEAG
                     QAACTQILSRKGIGYNEADGSFTYLPQKRISFSSLATDIPIPDLKRLAGHIAERLDVL
                     AQCESPVYIADILESVLSVIKNVDTGGKRARENFKQILKTEFIYAAAAVLKNKTKWEI
                     PENTRNLDSDIICTYICEVFLKSHLLGNSFPMMRPREVKQMPEPVFDKVLFAEQRARQ
                     LEVIRTSKYIFAIAPYYTDDIPFSLRRFLSEDKLYTSYNRYYTAIHIPVRNITQNDAL
                     RFANGLKQILSLQSGVSWEIIDMMDRIEENYDKKALPLLFSPFPAQVERTQAIAARLD
                     QFERLLGDTVLDPFYYCLTRMAKGEEDLKYIYIAFRQSFGGIFNSFENFRLLPALWMS
                     RDAENMSDRMNAYISAMEDRRREILSIRQGKPEEEFSRKMVVCLIDLENCLEKHLEQF
                     KPVSESIEACTAKLQEQPSFFNRLMKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLY
                     RHREVVSVHNRKADYAEKGKEQIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLHIF
                     SWIPR"
BASE COUNT          476 a          509 c          426 g          414 t
ORIGIN      
        1 aaatgaacat ttcgagcaag accttatccc ttacgccgtc cgtttcaggc tcaatcaaat
       61 ccttcccgaa ctcgactgcc attggcagga aaatctgaca gaaatcttca aaacactttc
      121 tcccgaagcc gggcaggccg cctgtacgca aatcctttcc cgaaaaggca taggctataa
      181 cgaagccgac ggttctttta cttaccttcc ccaaaaacgc atttcgtttt cttctttggc
      241 aacagacatt cccatccccg acctcaaacg gctggcgggg catattgcgg aacgcctgga
      301 tgtattggcg caatgcgaat cgccggttta tattgccgac attctcgaaa gcgtgctgtc
      361 cgttatcaaa aacgtcgata ccggaggcaa gcgcgcacgg gaaaatttca aacagattct
      421 gaaaaccgaa ttcatctacg ccgccgctgc ggtattgaaa aacaaaacca aatgggaaat
      481 ccccgaaaat acccgcaatc tcgattcgga catcatctgt acctatatct gcgaagtgtt
      541 cctaaaatcg cacctgctcg gcaacagttt tccgatgatg cgcccgcgcg aagtgaagca
      601 gatgcccgaa ccggtgtttg acaaagtatt gtttgccgaa cagcgcgccc gccagttgga
      661 agttatccga acgtcaaaat acatcttcgc catcgcgcct tattacaccg acgatattcc
      721 gttttccctg cgccgcttcc tgtcggaaga caaactttat accagctaca accgctatta
      781 caccgccatc catatccccg tccgcaacat cactcagaat gatgccctgc gttttgccaa
      841 cggtttgaaa cagattctct cgctgcaatc cggcgtgagc tgggaaatca tcgatatgat
      901 ggacaggata gaggaaaact acgacaaaaa agccctgccg ctgttgtttt cgccattccc
      961 cgcgcaagtc gaacgcacgc aggcgattgc cgccagattg gaccaattcg agcggctgtt
     1021 gggcgacacc gtcctcgatc cgttctacta ttgcctgacg cgtatggcga agggcgagga
     1081 agatttgaaa tatatttata tcgccttccg ccagtctttc ggcggcatct tcaattcgtt
     1141 tgaaaacttc cgcctgctgc ccgcgctgtg gatgagcagg gatgcggaaa atatgtccga
     1201 ccgcatgaac gcctatattt ccgcaatgga agaccgccgc cgcgagatat tgtccattcg
     1261 gcagggaaag cctgaagaag aattttcccg caagatggtc gtctgcctga tcgacttgga
     1321 aaattgtttg gaaaaacacc tcgaacagtt caagccggta tcggaaagca tcgaagcctg
     1381 taccgccaaa ctgcaggagc aaccgtcgtt cttcaacagg ctgatgaaaa ccaacgacaa
     1441 gctcaacagg cagattgacg tgttgcagaa acagtcggca gccatccaca acgaagccta
     1501 tatcgaaatg aacacgctgc tttaccgcca ccgcgaagtg gtttccgtcc acaaccgcaa
     1561 agcagattat gcggaaaagg gcaaagagca gattgcgctg ttcccgcgcg gattgaacgg
     1621 catcaccaag ctgcccgccg ccgtcctgtt gcccgagcgt ccctaccatt ttgatatgaa
     1681 ggaggttctg catatattct cctggatacc gaggtaggcg tatcggctgc cggataaaaa
     1741 tgcaccccgt cattcctgtc tgtacggggg tagcggcagt gttttcctgc ttcgttttat
     1801 agtggattaa caaaaaccag tacgg
//
LOCUS       DAXHPG010000234         1790 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXHPG010000234 DAXHPG010000000
VERSION     DAXHPG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.234"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(148..804)
                     /locus_tag="ACFM6F_002079"
     CDS             complement(148..804)
                     /locus_tag="ACFM6F_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC6165423.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALAAAAILLLS
                     "
     gene            869..1759
                     /locus_tag="ACFM6F_002080"
     CDS             869..1759
                     /locus_tag="ACFM6F_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC6165424.1"
                     /translation="MFPVFHLSGESRRRMLQAASRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWMVGCGLGTDKAAVGTLAGILAEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITKSM"
BASE COUNT          402 a          421 c          588 g          379 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata aaacggtttg ggacggaaaa acggcctgaa
       61 gccgtttcgg gcattcagac cgtttgcgcg gagaggggat gccgtccgaa gggcgaaaag
      121 ggcttcagac ggcattgatg tcgggtttca ggacaggagc aggatggcgg ctgcggcaag
      181 cgcggcaacc gataatgcgg cggcaagcgc ggttttgccg gcaaagcgga ttgaggtttt
      241 gccttcgatg tatttgaagc cggttatcat cgggaggacg aggtttttct ttttgaacac
      301 gcggtatgcg gcgacggcgg cgatatgaat ggcggaaaaa acggcgagca gcttgaaaaa
      361 gttgaggtgg attttccgca taaggctgcc cgtatgctcg gaaaccaaat ggttgaggta
      421 gccgttggtg ctgaaggtgt tttcatcggc ggcaaaaagc cctgtgccga cttgaaatga
      481 gacggcggcc aaaagcgcga cgaccatcag tgcgcccaag gggttgtgtc cgggttggac
      541 gtgttcggga ataccgtttt tcatatactc tctgataccc gaccatccgc ggacgaaacg
      601 ggagaaacgg gcggtatcgc tgccccaaat gccccagcag aggcggaata cgagcaggaa
      661 aaggataaac agcccgacgc gcgtgtgcca ttgcagcata tcgccgccgg ttttcgcgct
      721 ataccacata aagggtaggg atgcggcaag cagccagtgg aaaaggcggg tggggaagtc
      781 ccagactttg gttttgtttt tcataattgg tttccggcgg tagaatcggt ttgttttcga
      841 gccttaattt taaccgattg gaggggaaat gtttcccgtt ttccatcttt caggcgaaag
      901 ccgccgccgg atgcttcagg cggcatcgcg ttttccctat gttttcaaag cccgtgcgga
      961 agattcgcac aaagggactt tcggcacgct cgccgtagtc ggcggatcgg cagggatgag
     1021 cggcgcgccc gtattggcgg catcggcggc gatgtatctc ggctgcggca aggtgtgggc
     1081 gggtttcaat caggatgcgc ttccgtttgc cgtcattgcc ggttttcccg aaattatgtt
     1141 ggacacggca gacggtttga ccaagcgtca aggcataaac gcttggatgg taggttgcgg
     1201 cctgggtacg gataaagcgg cggtcggaac gcttgccgga attttggcgg aacacacgga
     1261 caagcccgtc cttttggatg cggacgcgct gaacatatta tcaaccgatg ccgaaacccg
     1321 aaatctggcg cgcgggtgta aaaacctgat tttaacgcca caccccgccg aagccgcgcg
     1381 cctgcttgga acgacggttg cacaggttca ggcggatcgg acggcggcag tgaggaagat
     1441 aggggcaatt ttcggcgcaa ccgtggtttt aaaggggcac aaaacattgg ttgctgcgtc
     1501 cgatacggaa atctatgtca acgaaagcgg caacgcggga ttggcaacgg cgggcagtgg
     1561 cgacgtattg ggcggcatca tcggcagtct gctcgcacag ggcgtgccgg tttttgaagc
     1621 cgcctgcgcg ggcgcgtggc tgcacggcgc ggcggcggat gtcataaaag aatcggcagg
     1681 cattgcggca gggctgttgg caggggaaat cgctccggcg gcaaggtggc tgcgcaaccg
     1741 gataactaaa agtatgtaag aagatatagt ggattaacaa aaaccagtac
//
LOCUS       DAXHPG010000235         1786 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXHPG010000235 DAXHPG010000000
VERSION     DAXHPG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.235"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(194..1363)
                     /gene="porA"
                     /locus_tag="ACFM6F_002081"
     CDS             complement(194..1363)
                     /gene="porA"
                     /locus_tag="ACFM6F_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin PorA"
                     /protein_id="HFC6165425.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEAQ
                     AANGGASGQVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGG
                     ATQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDM
                     PVSVRYDSPDFSGFSGSVQFVPIQNSKSAYTPAHVVVNNKVATHVPAVVGKPGSDVYY
                     AGLNYKNGGFAGNYAFKYARHANVGRNAFELFLIGSATSDQAKGTDPLKNHQVHRLTG
                     GYEEGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDLIERG
                     KKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          457 a          462 c          411 g          456 t
ORIGIN      
        1 tatatgtgca aaccaaaccg aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tactgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgagcagatt
      121 ggcagtcaga ttgcaatcga agaatgaatg caagccgtca aaaacaaagc tatccgcttc
      181 accgccccga tatttagaat ttgtggcgca aaccgacgga ggcggcatta atttgagtgt
      241 agttgccgat gccggtattg cgtttcagcc aagcgccaga cacgatggcg gaagtgcgtt
      301 tggaaaaatc ataatcaacg ccggcgatga tttgatcgta gctggtattt tcgccttttt
      361 taccgcgttc gatcaagtcg aaaccatggg catagctgat gcgtggaact gcattaccga
      421 agcggtagga agcagtcgcg gcaatttcgg tcgtactgtt tttggttttg tcggcatttt
      481 cagacaaatc caactgagcc gccaaggcga gattcaagcc gccttcctca tagccgcccg
      541 tcaggcggtg tacctgatgg tttttcaagg gatcggtacc tttggcttga tcactcgtcg
      601 cgctgccgat caagaacaac tcaaaagcat tacgtccgac attggcgtgt ctcgcatatt
      661 taaaggcata gttcccggca aaaccgccat ttttgtaatt cagaccggca taatacacat
      721 ccgatccggg cttgccgaca acagccggaa cgtgagtagc aaccttgtta ttcacaacaa
      781 catgagccgg cgtataggcg gacttgctgt tttggatcgg aacgaattga acgctgccgc
      841 tgaaaccgga aaagtccgga gagtcgtagc gtacagaaac cggcatatcg tcgtggcgtt
      901 tgaaaatacc caattgcgaa gccacatcat tgttgctgtc ccaaggatca atggcttggc
      961 tggcatcgtc aaactgattc gcaacgcgac cggcgcgcag cgtaccgaat tcgcctgcca
     1021 agccgataaa ggattccctg ttgccccact gggtcgcgcc gccgccggca acggatacgt
     1081 cttgctcaag ctgccaaaca gctttcagac cttcgcccaa atcctcgctc cccttaaagc
     1141 cgataaacga gccgaaatca ctgattttcg tcctgatgcg gcttttggcc ttagtaactt
     1201 ttacctgacc gctcgctcca ccgttagcgg cttgtgcttc agtcaattgc agctggtagt
     1261 tcctgccttc cacgccggct ttgatttcgc cgtataggct gacatcggca acggccgcaa
     1321 gcggcagtgc ggacaatacg agggcggtaa gtttttttcg catatcggct tccttttgta
     1381 aatttgataa aaacctaaaa acatcgggca aacacccgat acgtcttcaa ttataccccc
     1441 cccccccgca aaaaaccatt tttcagaaca aatatctgat aaatgccgca acctttattt
     1501 taaaaatgat tatattttga tataaaacaa tagcttattt tttcaaaaac gttgtgtttc
     1561 tacaacacaa ttcaagcgca gacctcgtgc gagccgatgc gctgctgccc ggatgcagtc
     1621 tcggcttttt aaaacgccat aaaaaaacac acgcggcact ttatagtgga ttaacaaaaa
     1681 ccagtacggc gttgcctcac cttagctcaa agagaacgat tctctaaggt gctgaagcac
     1741 caagtgaatc gattccgtac tatctgtact gtctgcggct tcgccg
//
LOCUS       DAXHPG010000236         1734 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXHPG010000236 DAXHPG010000000
VERSION     DAXHPG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.236"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            356..1660
                     /locus_tag="ACFM6F_002082"
     CDS             356..1660
                     /locus_tag="ACFM6F_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC6165426.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          355 a          435 c          511 g          433 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cggcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgcgta tttttaagcg ggatgcagat agacgctgcc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtctgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggctat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga taaa
//
LOCUS       DAXHPG010000237         1703 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXHPG010000237 DAXHPG010000000
VERSION     DAXHPG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.237"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            63..1436
                     /locus_tag="ACFM6F_002083"
     CDS             63..1436
                     /locus_tag="ACFM6F_002083"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6165427.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1616..>1703
                     /locus_tag="ACFM6F_002084"
     CDS             1616..>1703
                     /locus_tag="ACFM6F_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HFC6165428.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYS"
BASE COUNT          441 a          559 c          407 g          296 t
ORIGIN      
        1 tttccccatc ctatcagttt cccgtttcag acggcataag gtctgaaaga aagactacaa
       61 tcatgagtaa tccattttcc tctttaggtt tgggtacgga actggtttcc gcactgaccg
      121 cgcaaggtta cgaaaacccg acgcccatcc aagccgccgc cattcccaaa gcactcgccg
      181 gtcatgattt actagccgcc gcccaaaccg gcacaggcaa aaccgccgcc tttatgctgc
      241 ccagtctgga acgcctcaaa cgttacgcca ccgccagcac ctcgcccgcg atgcaccccg
      301 tgcgtatgct cgtgttgacc ccgacgcgcg aactcgccga ccaaatcgac caaaacgtgc
      361 agggctacat taaaaacctg ccgctgcggc ataccgtcct cttcggcggt gtcaacatgg
      421 acaaacaaac cgccgacctg cgtgccggct gcgaaatcgt cgtcgccacc gtcggacggc
      481 tgctcgacca cgtcaaacaa aaaaacatca gtttaaacaa agtcgaaatc gtcgttttgg
      541 acgaagcaga ccgcatgttg gatatggggt ttatcgacga catccgcaaa atcatgcaga
      601 tgctgccccg ccaacgccaa accctgctct tttccgccac cttctccgca ccgatacgca
      661 aactggcgca agacttcatg aacgcgcccg aaaccgtcga ggttgccgcg caaaacacca
      721 ccaacgccaa cgtcgaacaa cacatcatcg ccgtcgatac cattcagaag cgcaacctgc
      781 tcgaacggct gattgtcgat ttgcatatga accaggtcat cgtgttctgc aaaaccaaac
      841 aaagcgtcga ccgcgtaacg cgcgaactgg tgcgccgcaa cctgtccgca caggcgatac
      901 acggcgaccg ttcccaacaa agcaggctcg aaacactcaa cgccttcaaa gacggcagcc
      961 tgcgcgtcct cgtcgccacc gacatcgccg cgcgcgggct ggacattgcc gaactgccct
     1021 tcgtcatcaa ttacgaaatg cccgcccagc ccgaagacta cgtccaccgc atcgggcgca
     1081 cggggcgcgc gggcgcggac ggcgtggcga tttccctgat ggacgaatcc gaacagaaaa
     1141 tgtttgaatc cattaaagag ctgaccggca acaagctgct catcgagcgc atcgagggct
     1201 tcgagccgca atggtgggaa cagggcggcg caaaaccgga aaaacccgaa atgcgcgaac
     1261 caagacaacg caaccgctac gaatccgcca aagcgcaacg cgaaaaaaac acccggccgg
     1321 aaaatgcggc aaacgatgcg ggcgcggctt gcggaaaaat cgccggacgc agccgccgaa
     1381 gccgccggga acaccggacg tgcgccctgc tccaaccgcg ttacggcgta aaataaccct
     1441 gaaaatcaaa tgccgtctga acatttcccg tttcagacgg catttttcaa accagactaa
     1501 cgcattgggc gcaaccgccc ataccggata aatttctgcc gcaaacagtt tcagacggca
     1561 tttgctgcct gtacaatatc atttcctttt ccactcaaac catcaggatt tcccaatgtc
     1621 cacccaattc gatgtcgccg taatcggcgc aggtccggca ggctcggtcg catccgccct
     1681 gctccgcaaa aaaggttata gtg
//
LOCUS       DAXHPG010000238         1554 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXHPG010000238 DAXHPG010000000
VERSION     DAXHPG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.238"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>1554
                     /locus_tag="ACFM6F_002085"
     CDS             <1..>1554
                     /locus_tag="ACFM6F_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6165429.1"
                     /translation="YIADIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNINWNQVQLAY
                     DKWDYKQEGMTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTG
                     VAAGTAATTGVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSS
                     TVRQAATAAVTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAI
                     NGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVANKGK
                     CRDGAIGAAVGEMVGETLLDGRDVGKLSPQERQKVIAYSQIIAGSVVALVKGDVNTAA
                     NAATVAVENNSLLARRRVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGIAILR
                     TPGEPWTRHDVQTYRQYYNQLRESRGFAVDPIYRIRINNGNEFNRIMSSKYPYNELYV
                     ANPKSATGYFRVDSYNPATEEIISRKFTQFSQIQESTGIGYIKEAVRKYSPGAVISNV
                     PSTPTTIRGR"
BASE COUNT          476 a          393 c          398 g          287 t
ORIGIN      
        1 tatatcgccg acatccccaa aggcaacctc aaaaccgaaa tcgaaaaact cgccaaacag
       61 cccgagtatg cttatttaaa acagcttcaa gttgcgaaaa acatcaactg gaatcaggtg
      121 cagcttgctt acgataaatg ggactacaaa caggagggca tgacacccgc agcagcagct
      181 gtcgtcgtta tcgtcgtaac tgtattgacc tacggcgcac tgtccgcccc ggcagccgcc
      241 ggaacggcgg gcgcggcagg cgcaggagcg ggaggagccg cagcaggaac ggcagccgga
      301 actggagtag cagcaggaac ggcagccaca accggagtag cagcaggcac atcagctgca
      361 gctatcacca cagccgcagg caaagccgca ctggccagtc tcgccagcca agccgcagtt
      421 tccctcatca acaacaaagg agacataaac cataccctga aagaactggg caaaagcagc
      481 accgtcagac aggccgccac cgccgccgta accgcaggcg tactgcaggg cataagcggg
      541 ctgaacaccc aagcagccga agccgtcagc aaacattttc acagtcccgc agcaggcaaa
      601 ctgaccgcta acctgatcaa cagcaccgct gccgcaagtg tccataccgc catcaacggc
      661 ggcagcctaa aagacaactt gggcgatgcc gcactgggtg cgatagtcag taccgtacac
      721 ggagaagtag cgagcaaaat caaatttaat ctcagcgaag actacattgc ccacaagata
      781 gcccatgccg tagcaggctg tgcatcggcg gtagcaaata aaggcaaatg tcgggacggc
      841 gcaatcggcg cggcagtcgg cgagatggtg ggagaaaccc tgttggacgg acgcgatgta
      901 ggcaaactgt caccccaaga acgccaaaaa gtcatagcct actcgcagat tatcgcaggc
      961 agcgtagtgg cattggttaa aggggatgtg aatacggcgg cgaatgcggc tactgtggca
     1021 gtggagaata atagtctttt agctcgcagg agggtaaata tacgttggac tccgcgacaa
     1081 gaattggaac atgaatatgc cattcttgaa atccaggcca ttaccaatca aatccgaagg
     1141 ctggatccga aatttaacgg gattgctatt ctgaggactc ctggagagcc gtggacaaga
     1201 catgatgtac aaacatacag gcaatattat aatcaattaa gggaatccag aggctttgct
     1261 gttgacccaa tttatagaat caggataaac aacggcaatg aatttaaccg tatcatgtca
     1321 tcaaaatacc cttataatga gctttatgta gccaatccta aatcggcgac ggggtatttt
     1381 agggtagatt cgtataatcc tgcgacagag gaaattattt caagaaaatt tacccaattt
     1441 tctcaaatcc aagaaagtac ggggattggt tatatcaagg aggctgttag aaaatatagc
     1501 cctggtgctg tcatttccaa tgttccaagt acacctacta cgataagagg aaga
//
LOCUS       DAXHPG010000239         1533 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXHPG010000239 DAXHPG010000000
VERSION     DAXHPG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.239"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..217)
                     /locus_tag="ACFM6F_002086"
     CDS             complement(<1..217)
                     /locus_tag="ACFM6F_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HFC6165430.1"
                     /translation="MTYANFNINGFGDVKLTPYSPLLGYKAWDSFIGSIQSLSDLIYN
                     VDNNRNKMEITVNNAIQAADSFLSSNWK"
     gene            1252..1428
                     /locus_tag="ACFM6F_002087"
                     /pseudo
     CDS             1252..1428
                     /locus_tag="ACFM6F_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin B"
     gene            1431..1533
                     /locus_tag="ACFM6F_002088"
                     /pseudo
     CDS             1431..1533
                     /locus_tag="ACFM6F_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          521 a          287 c          220 g          505 t
ORIGIN      
        1 tcttccaatt actgcttaaa aagctatctg cagcttgaat agcattatta acagtaattt
       61 ccattttatt tctattgtta tccacattat agattaaatc agataaggat tgaatagaac
      121 caataaatga atcccaagct ttatatccca agagtggtga atagggtgtt aatttcacat
      181 ctccaaaacc gttaatatta aaatttgcat atgtcatatc tgctttcctt tcaagtaaat
      241 taaaaaaata acgtgaggaa ttaaaattaa aaataaatta attatcgact caatataata
      301 tgaatcaatt agctctctta tggtaaatat aagaggtaaa aatactaaaa ataaactaat
      361 aaagttcaga aaaatagtaa atggaatatt ttcatatgga aaaattctac aaaatacaag
      421 taatattcca tgacatatta gcaaatgagt aaaatttatt ataaaatata taaatatttc
      481 cccatcgtaa gatatattca aataaagaaa tataaacaat atgaagtata taaaatatat
      541 tttttggcat ctaaaaaata aacttttagc aatcatatat aaatagcttg tagaataaaa
      601 aacgaaaatt attaattatc gtaggtgtgc atttattcca acacggtttc atgcagctta
      661 aaaaccaaaa attctttctc cctttatcct ttcttaaaaa tagcgttttc aaattctgca
      721 acgacaggtt tcattcaagt aggactgccg tgattgcatc aattgcttcg cggcatggca
      781 tccatatcac acaacaggac atttattctc gggtaagtcc accttcatcc tcttccgcca
      841 gcaggaacag ttacaggcta cctaaaatat gctctgtgct cggatagtaa atatttcctt
      901 tctcactatt gtttgtcatt taatcgacag tctactcttt ctctaaaatt tcgtcaataa
      961 tagttaaata tatttaaata catttaaaat aataatatac acctgtttca gtctgtctaa
     1021 aacacgggaa aatatgttat ttgatcacaa cggtgacttt gggcagcctg taccttcaat
     1081 aacgcgtgga aaatagatat atacttatat cgtattcgag gctgtcttgt ttccctcaaa
     1141 caaccattct tccacacttt tgctttacaa ttttcgtgtg tatccttaca attaccccta
     1201 ttgtttttgt atattccttt gccaaccgtt attgctatcc aatctaatat tatggctatc
     1261 gtatctacac cacaccccgc tctttccgcc ctcatcatcc tcgcccatta tcacggcatt
     1321 gccgccaaat ctttgggatt gaaggcaaag gtagtacgcc agcctgtcaa acgtttggct
     1381 atggtgacct tacctgcatt ggtatggtgt gatgacggca accattttaa ggaaaagtgg
     1441 ttgttttcgg cattctgaaa cgcaacggac gggtctatac cgttgtggtg gataatgcca
     1501 agtctgaaac gttactccct gtcatcaaaa aga
//
LOCUS       DAXHPG010000240         1517 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXHPG010000240 DAXHPG010000000
VERSION     DAXHPG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.240"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(10..456)
                     /locus_tag="ACFM6F_002089"
     CDS             complement(10..456)
                     /locus_tag="ACFM6F_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165431.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNI"
     gene            complement(1021..1224)
                     /locus_tag="ACFM6F_002090"
     CDS             complement(1021..1224)
                     /locus_tag="ACFM6F_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165432.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
BASE COUNT          516 a          280 c          204 g          517 t
ORIGIN      
        1 gtctagagtc tatatattac atttattttt agggtctgct agccaatttc ttgttccctt
       61 cattatttta tcttctgaaa gaaaattatt tttttccatg ctattaatat taatgatatg
      121 atttttattt aaaataattt ttccatataa aatttccggt ttgtaatagc aagagtctga
      181 agcaaatcct tgcccgtcaa tataagaaaa ataaatgtta tcatcctcct tataatattc
      241 taaaaccaat ccggatatta ttttatcaaa attcccttca tttccatctt tcatgactaa
      301 aatataatta gcaggccaac ctttatcgta aacatattca aactgatagt tcccgaaact
      361 ctcgatatcc gaactaaaaa agaagaaaag caaattaata gccaatacaa aaacaaataa
      421 gaacaaaaat agcaaaattt tcaacttagt taacaatatt tacctctcct ttaaattcaa
      481 tcctgaaagg taccccttac cctgggctac caattatagt ttccatattt ctaaatattg
      541 tttttacatt acttttttct ccccccaaag gaatgcattt taaaatcatg cttttcaggt
      601 gctaatcgat acttaccatt aacatcttta atcacagata tatttccatg tatagcccaa
      661 cgtgaaaaat ctgagtatta tatacagtta taccttactg aaacaaagct agctgtacat
      721 atctgtacca tatttttact acaaacaaaa aatatttata attattaagg ataagataaa
      781 aacccccaac gccagattaa ttcttaataa tttcaaataa aaaatatttg agattttttc
      841 ttttccaaat aatatattga aatatttaaa gttaaggtag cttgatttaa ttagaaaatc
      901 aaaaaaaacc attaattaat ttttcttctt taaatatttt atatgatatt ataaaattaa
      961 ataacattgc gaaaacatat gaaatcatca aaataccaaa tatatagtgg gaatgcatta
     1021 ttatttagct ccttcttttg gctctgacaa attgacagtc cattgatatc caatgcttgc
     1081 atttgcaatt gctgacgcac ctgcccctgt tttccctgtt agtatcaatg gttcttttct
     1141 tgcaagatgt gcacctgcac aaccacctaa tactaaacca ccacctattg taaatccttc
     1201 ctgttgtatc tctaatgata acattttctt cttgtgccgc ccttaataca gattgtggaa
     1261 ttggattgac ctgagcagga acttctaaaa taagttttcc ttcaagctgt cttcctctta
     1321 gcagattagg tgtacttgga acattggaaa tgacaaagtg agtacgcaaa ccacctgaag
     1381 gcgatgtgtt tgaacctgtt gaaagcggcc aacaggctaa atgcgcccgc tgccgcctaa
     1441 aaggcagccc ggatgcctga ttatcgggtg tccgaggagg attaaggggg tatttgggta
     1501 aaattaggag gtatttg
//
LOCUS       DAXHPG010000241         1499 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXHPG010000241 DAXHPG010000000
VERSION     DAXHPG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.241"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            30..1424
                     /locus_tag="ACFM6F_002091"
     CDS             30..1424
                     /locus_tag="ACFM6F_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFC6165433.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVFPSIRYEADSATDFTGIPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNERHSFGAGIIAQHTSAELRKYADWGIPKKAQMLQATPSNPTAAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAMAKQLWNSNM
                     LAPLGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKE
                     KTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
BASE COUNT          398 a          495 c          342 g          264 t
ORIGIN      
        1 ccaccgtccg cctttttacg gaaaccgaaa tgaccccttc cgcactgaaa aaaaccgtcc
       61 tgctgctcgg cactgccttt gccgccgcat ccgcacaagc ctccggctac cacttcggca
      121 cacagtcggt caacgcgcaa agcacggcga atgccgccgc cgcagaagcc gccgacgcat
      181 cgaccatctt ctacaacccc gccggcctga ccaaactcga cagcagccag atttccgtca
      241 acgccaatat cgtatttcca agcatccgtt atgaagcgga ttccgccacc gactttaccg
      301 gcattcccgt ccaaggttcg aaaagcggca aaatcaccaa aaccacggtt gcgccccaca
      361 tctacggcgc atacaaagtc aacgacaatc tgaccgtagg cttgggcgtg tacgtcccct
      421 tcggctctgc caccgaatac gaaaaagatt ccgtattgcg ccacaacatc aacaaactcg
      481 gtctgaccag catcgccgtc gaacccgtcg ccgcgtggaa actcaacgaa cgccattcct
      541 tcggcgcagg catcatcgcc caacatactt ctgccgagct gcgcaaatat gccgactggg
      601 ggatcccaaa aaaagcgcaa atgctgcaag caacaccttc taatcctact gccgctgctc
      661 aaatcaaagc cgacggacac gccgatgtca aaggcagcga ttggggcttc ggctaccaac
      721 tggcgtggat gtgggacatc aacgaccgcg cacgcgtggg cgtgaactac cgttccaaag
      781 tctcacacac gctcaaaggc gatgccgaat gggcggcaga cgacgcaatg gcgaaacaac
      841 tgtggaatag caatatgctc gcaccgctcg gctacacggc gaatgaaaaa gcccgcgtta
      901 aaatcgttac gcctgagtct ttgtccgtac acggtatgta caaagtgtcc gataaagccg
      961 acctgttcgg cgacgtaact tggacgcgcc acagccgctt cgataaggcg gaactggttt
     1021 ttgaaaaaga aaaaaccatc gtcaacggca aatccgaccg caccaccatc acccccaact
     1081 ggcgcaacac ctacaaagtc ggcttcggcg gttcttatca aatcagcgaa ccgctgcaac
     1141 tgcgcgccgg catcgctttt gacaaatcgc ccgtccgcaa cgccgactac cgcatgaaca
     1201 gcctgcccga cggcaaccgc atctggttct ccgccggcat gaaataccat atcggcaaaa
     1261 accacgtcgt cgatgccgcc tacacccaca tccacatcaa cgacaccagc taccgcacgg
     1321 cgaaggcaag cggcaacgat gtggacagca aaggcgcgtc ttccgcacgt ttcaaaaacc
     1381 acgccgacat catcggcctg caatacacct acaaattcaa ataaacggcc cgccgtttga
     1441 ttgtagaaat gccgtctgaa acagatttcc gtttcagacg gcattgtttt aggctcgat
//
LOCUS       DAXHPG010000242         1498 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXHPG010000242 DAXHPG010000000
VERSION     DAXHPG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.242"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..372
                     /locus_tag="ACFM6F_002092"
     CDS             <1..372
                     /locus_tag="ACFM6F_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC6165434.1"
                     /translation="RLRRLVLIFVNPLYIIATTVPAQQTFLDAAELMQWSRETEWLGL
                     NVIAHRNFGKQHAQVEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQP
                     CICGSGKKFKACCGKYLKPVA"
     gene            complement(663..1457)
                     /locus_tag="ACFM6F_002093"
     CDS             complement(663..1457)
                     /locus_tag="ACFM6F_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6165435.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
BASE COUNT          393 a          459 c          322 g          324 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat acatcatcgc tactaccgtt
       61 ccggcacaac aaacattcct cgatgccgcc gaactgatgc agtggagcag agaaaccgaa
      121 tggctgggct tgaatgtcat cgcacaccgc aacttcggca aacagcacgc ccaagtcgaa
      181 tttgaagcct acttccgaga cggacaacac cgatctgcgc atcacgaact gtccggcttc
      241 gtcaacatcg gcggacaatg gtattttatc gatcccaccg ttccgcatcc tgcgatgaaa
      301 caaccctgca tttgcggatc gggcaaaaaa ttcaaagcct gctgcggcaa atatctgaaa
      361 cctgtcgcat aaaaatgccg tctgaacgtt cagacggcat ttttaacgcg caaaaaaaac
      421 cattcatacc aagggtaagt atgaatggtc aatacattgc gggaaaacgt cttacttgct
      481 gcactgccga aaagggagaa acggcagcgg taatcagcgg aaaggattgt acccgtataa
      541 atgttaagta acgttaatta tgaaaatata accgatgttt cggcacacaa tccgcaactg
      601 tccggcgcag gcggcggcaa agcctgttta ttcaaaccct tattcctgcc catctgtcca
      661 ccttactggt tttcccgtat ccacccgacc cagcgttcaa acagcttcgg ttcgagcgcg
      721 gcaacgaccg agcgtttgaa cacgtgttca ccactccaaa acccgtcgcc ttccaaagtc
      781 gtcagcctgc cgcccgcctc ctcgaaaatc aacgcacccg cagcatagtc ccacagcttc
      841 tgcccaccgt gaacataaac atcataacgc ccgcacgcca gataacacca atccaacgta
      901 ctgctgccca tactccgtat cgttccaaaa ggcgcgagcg tactcatacg gctggaaagt
      961 ttgcccgaac gcaaatattt gatttccacg cccgcaatcg cctcattgag ttttttatcc
     1021 acgagacgca aaggcaggcg ggttccatta agaaacgcac cctgcccccg ttcggcataa
     1081 aaacattcgc cgctgaccgg attgtaaatc acacccaatt cggcgcgccc gttgcggaca
     1141 aacgccaccg acacggcaaa atgcggcagc ccgttgacaa aattgttcgt cccgtctatc
     1201 ggatcgacaa tccacagccc cttttccccc gaatattgtt cccacaaagc cgactgttcc
     1261 tgccgcgaca tttcctcgcc caacatcgga ctgtcgatta aaagcggcaa cgcggcggca
     1321 aaagccgtct gcgcggcaat gtccgcctcg ctcaacatcg aaccgtcttc cttgcggtga
     1381 gacggcgtat tcaaaaaacg cggcataatt tcggtttgcg cgatatggcg cacgactttc
     1441 tgcaaacggt gtaacacttc ctactgtcct catattttga acttgcggca cgcgaacg
//
LOCUS       DAXHPG010000243         1491 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXHPG010000243 DAXHPG010000000
VERSION     DAXHPG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.243"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..819
                     /gene="mafA"
                     /locus_tag="ACFM6F_002094"
     CDS             <1..819
                     /gene="mafA"
                     /locus_tag="ACFM6F_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6165436.1"
                     /translation="RAAVKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIR
                     GGYHNNPESATQYSYPAYDTTATTKSDALSSVTTSTSLLNAPAAALTKNSGRKGERSA
                     GLSVNGTGDYRNETLLANPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFG
                     TVRSRTELHLYNAETLKAQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPY
                     SVGKTVKASDRLMVDFSDITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            823..>1491
                     /gene="mafB"
                     /locus_tag="ACFM6F_002095"
     CDS             823..>1491
                     /gene="mafB"
                     /locus_tag="ACFM6F_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6165437.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGSVNEGFTVYRLNWKGHEHHPADGYDGPQGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANKKIFEHNAKL
                     DRWGN"
BASE COUNT          403 a          557 c          324 g          207 t
ORIGIN      
        1 cgcgccgccg tcaaagaaat ggacttgtcc gccctgaaag gacgcaaagc cgccctttac
       61 gtctccgtta tgggcgacca aggttcgggc aacataagcg gcggacgcta ctctatcgac
      121 gcactgatac gcggcggcta ccacaacaac cccgaaagtg ccacccaata cagctacccc
      181 gcctacgaca ctaccgccac caccaaatcc gacgctctct ccagcgtaac cacttccaca
      241 tcgcttttga acgcccccgc cgccgccctg acgaaaaaca gcggacgcaa aggcgaacgc
      301 tccgccggac tgtccgtcaa cggcacgggc gactaccgca acgaaaccct gctcgccaac
      361 ccccgcgacg tttccttcct gaccaacctc atccaaaccg tcttctacct gcgcggcatc
      421 gaagtcgtac cgcccgaata cgccgacacc gacgtattcg taaccgtcga cgtattcggc
      481 accgtccgca gccgcaccga actgcacctc tacaacgccg aaacccttaa agcccaaacc
      541 aagctcgaat atttcgccgt tgaccgcgac agccggaaac tgctgattgc ccctaaaacc
      601 gccgcctacg aatcccaata ccaagaacaa tacgccctct ggatgggacc ttacagcgtc
      661 ggcaaaaccg tcaaagcctc agaccgcctg atggtcgatt tctccgacat caccccctac
      721 ggtgacacaa ccgcccaaaa ccgtcccgac ttcaaacaaa acaacggtaa aaaccccgat
      781 gtcggcaacg aagtcatccg ccgccgcaaa ggaggataaa ccgtgaaacc gctgcgcaga
      841 ctgacaaacc tccttgccgc ctgcgccgta gcggccgccg ccttcggaca gcccgccctc
      901 gcggcggact tggcgcaaga cccgttcatt accgataacg cccaacggca gcactacgaa
      961 cccggaggca aataccacct cttcggcgac ccgcgcggca gcgtttccga ccgcaccggt
     1021 caaatcaacg tcatccaaga ctatacccac cagatgggca acctgctcat ccaacaggca
     1081 aacatcaacg gcacaatcgg ctaccacacc cgcttttccg gacacggaca cgaagaacac
     1141 gcccccttcg acaaccacgc cgccgacagc gcaagcgaag aaaaaggcag cgtcaacgaa
     1201 ggctttaccg tataccgcct caactggaaa gggcacgaac atcatcccgc cgacggctat
     1261 gacggcccgc agggcggcaa ttaccccaaa cctacgggcg cacgagacga atacacctat
     1321 cacgtcaacg gcacagcccg cagcattaaa ctcaatccga ccgacacccg cagcatccgg
     1381 caacgcatat ccgacaacta caacaacctc ggcagcaatt tctccgaccg cgccgatgaa
     1441 gccaacaaaa aaatattcga gcacaatgcc aagctcgacc gctggggcaa c
//
LOCUS       DAXHPG010000244         1452 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXHPG010000244 DAXHPG010000000
VERSION     DAXHPG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.244"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..486
                     /locus_tag="ACFM6F_002096"
     CDS             <1..486
                     /locus_tag="ACFM6F_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165438.1"
                     /translation="KQAAPKETINNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRV
                     RKGAGLLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFR
                     VLPDGRLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKF
                     Q"
     gene            505..879
                     /locus_tag="ACFM6F_002097"
     CDS             505..879
                     /locus_tag="ACFM6F_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6165439.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            1238..>1452
                     /locus_tag="ACFM6F_002098"
     CDS             1238..>1452
                     /locus_tag="ACFM6F_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6165440.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKT"
BASE COUNT          476 a          292 c          298 g          386 t
ORIGIN      
        1 aagcaagctg ctcctaaaga aacaataaac aatttggcaa atttagccaa agcagaacag
       61 cagattttat tccgtattgc ccaacgcgat acgcaactgg atgcatggaa gacgggattt
      121 aacaatagag taaggaaagg agcaggcttg cttgatgcaa gtaatattcc gataaccatt
      181 aacggaaaaa ccatcaaacc tgtacaagcc ataagcttaa agggagcacc cgtttacagc
      241 ggcgtaagcg aacaggagat ttttgcgctt tatcggcaga tgactggcca gaatccgaat
      301 tttagagttt tgcctgacgg aagattagca aatggcatta tcagtactgg agaatgggca
      361 ggaacaaaaa ttgcattaag aaatttttca aaaacagaga attcaactca agcacgatgg
      421 acattagatt tgcagaatcc tccatcattt attaaaggta ctaaattgga gcttaaattc
      481 caataattta caaaggattt taccgtggat gagaaacaaa aaattaagat tcttgatttt
      541 caaatcgatt tatcctcaat ttttaactct tataaaaatc aaatgggtat taatattcaa
      601 gataaaaact taaaaaacaa tttctgttct tttatggaag aactgttaaa tgacggttca
      661 atccgtttac atgattatac cgacggtatc ggaattcctc taactggaac ttcaaaagaa
      721 caagtgcaga aattgaaaga catatggcct actttggaag atgcccaagc aatatggcct
      781 gaagaccctt ggtattactt agaatggctt tggtgggata ttgcgtgtcc aatagatttg
      841 gccgatttgc cgaatattga tatttatgag caagcgtagg tatggttagc cgcctttagc
      901 ggcgtaaccg tacgcatatc agcaaacttt ataaaataac aaggccgtct gaaatctgtt
      961 tttcaacttt ttcagacggc cttgcaactt ggcatttcat tcgtacggtt acgccgctaa
     1021 aggcggctaa ccgtacctac gagctctgat aaaaatgatt tatggaagca agctgtagcc
     1081 tgcatgaaac ctaaaatcca tgcgtaaggt gtgtgcttca gcgcgcacgc gttccatgat
     1141 ttacggctca atgccgtctg aaaagctcaa ttttttcaga cggcatttgt tatgcaagta
     1201 aatattcaga ttccctatat actgcccaga cgcgtgcgtg ctgaagacac ctcctacgct
     1261 tgctgcagaa ctttcgggta aaaccggtgt gagcattagc gcaccgtatg ccaatgagaa
     1321 cagtcgcatc ctgctcagca ccacggatat cagttcggaa aacggcaaaa tcaaaattca
     1381 atcttacggt gaccaatatt actatgcgag acagagcgaa ctctatacct ttgaacgccg
     1441 cagctacaaa ac
//
LOCUS       DAXHPG010000245         1449 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXHPG010000245 DAXHPG010000000
VERSION     DAXHPG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.245"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..105)
                     /locus_tag="ACFM6F_002099"
     CDS             complement(<1..105)
                     /locus_tag="ACFM6F_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6165441.1"
                     /translation="MIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTRYL"
     gene            complement(200..871)
                     /locus_tag="ACFM6F_002100"
     CDS             complement(200..871)
                     /locus_tag="ACFM6F_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6165442.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNTVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMERGRGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          353 a          319 c          346 g          431 t
ORIGIN      
        1 gaggtaacgg gttctttgac ggttcaggta ctgctcgatc ggctgccaat caatcacctg
       61 gtccaacttc aatagcggga aacggtcgat gtgtttggca atcatggctt gggcggtttg
      121 ctggaagaag gtgctcttga gaaatcccct aaatgtcttg gtgggaattt aggggatttt
      181 ggggaatttt gcaaaggtct cagtctatgc ccgatataca attttgatac acaaacttgg
      241 aaatatcggt atcgtcgccg gagcgataga atgcggacag tttttcattg aattgcggca
      301 tctgcatttc ggagatttcc aagatgccac ggcctctctc catcatcaga ccattggcaa
      361 ataaggttgc cgttcgtttg ttgccgtccc aaaaaacctg ctgccgcata caataaagca
      421 taaaacggac ggctgcctcc gtcgtcgaac cgctttgcag tccgatattt tgtaacacgc
      481 gggccacttc aatttccttc actggattcg gggcatgacg ggaaccgtcc aatagcgtta
      541 cgccgaccga accggtacgg aaatcaccgg gtgccaaaga atcgtccttg gcaacagtgt
      601 tgttgatttt tttaaggagt gaaatatcga ccggttcgcc gtttgaaata tgcgaaatca
      661 catattgata ggcacgtttc aggttcaaga tggtttggat gtcttccagt gacgcggagg
      721 ctacattttt gccatggata atttgttcgg tctgcaacaa ggtcgtctga caattctcaa
      781 aacggctgag gttgtgaatt tgggcaacca atactttctt tgccaaaaaa atattttctt
      841 tcaaactcaa cttgtatttg tcgggaaaca cgataattcc ttaaataatt aaagcagtac
      901 gttaaatcat catcttatca tcactttcgg ggtttatgtt gcgtgcgtcc gttttctaat
      961 acggtttccc atcccgcgaa acgaggcgga ggcctgtgca aaaaacctgt tcacgaaatt
     1021 ttccgcatta acgatacgct gaaaatgcgc taaaaatgac tgtgtttgaa tatcggttga
     1081 tttcatccgt ttgtgtaacc tcgcgccggt tttgtcatga ttttgcatta tagtgaatta
     1141 aatttaaacc agtacagcgt tgcctcgcct taccgtacta tctgtactgt ctgcggcttc
     1201 gtcgccttgt cctgattttt gttaattcac tatatttttg tcatgcggat atttcacggg
     1261 atgacaaaac gggcgcaaaa aagcccgatt ggaaaatccg aatcgggctt ttttgtgatg
     1321 ttctgttcga tttgcgtact aactgccggc cggagcgtcc gcgcccggtt tggcaggtcg
     1381 gattctcgaa tccgacggct atttgagatg gcaggctttt gtcggataca agtatccgac
     1441 ctacggctt
//
LOCUS       DAXHPG010000246         1441 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXHPG010000246 DAXHPG010000000
VERSION     DAXHPG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.246"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(80..1417)
                     /locus_tag="ACFM6F_002101"
     CDS             complement(80..1417)
                     /locus_tag="ACFM6F_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6165443.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRALRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGSGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLGIL"
BASE COUNT          448 a          381 c          350 g          262 t
ORIGIN      
        1 tgaacgaaat aggctgaacc tgaagcaaaa atgccgtctg aaaggctttc agacggcatt
       61 tttgccgttt ggagggcttt taaaggatgc ccaaagaatt gatgaaaaca ataataatgg
      121 caatcggggc aaggtagcgc aaggtattca gccacagcag cagcactgcc cgtggtacgg
      181 tgctgcccga tccggcatct tttaacacag actgcttgtc ctgaatccag gcggtaaaga
      241 tggaaacact caaagcacca atcggcataa tgacggcgga aataacatag tcccacaaat
      301 caaaaatggt tttgccgaaa accttaaact caccccatac gccgaaagac agcgcggacg
      361 ggatgccgac aatgaaaata gccgtgccga taagccaagt gtgctttttg cgtttgcgct
      421 cgtcttggcg gatggttgag gcaatgaccg tttccaacat cgaaaatgcg gaagtcagcg
      481 tggcgaaaac gaccagaagc atgaataccg caaacaaaac cgtaccgaac ggcatcttca
      541 taaacactgc gggcaatacg ataaaaatca atcccggccc ctggctcggt tcaaaaccga
      601 aggcgaacac cgccggaaaa atcaccaggc cggcaagcag cgaaaccaag aggttcatcc
      661 acataatcgt atggccggaa cggaacatat cctgatcttt tcccaaatat gaagcgtagg
      721 taatcatggc ggaaacgccg atgctcaggg caaaaaacgc ctgtcccaaa gccgtaatca
      781 tcgtatcggc tttaaagtac gaccaattcg gtttgagcag gaaagacacg ccctccattg
      841 cacccggcag cgtcagcgaa cggattgcca gcgcaataaa gaggataaac agccccggca
      901 tcagataacg gtttgccttt tcaatgccgt ctgaaatccc gcctttgacc acccaaaccg
      961 taatcagcat aaacagtgcc tgataggaca gcgaacctgc cggattggaa atcgttgtgc
     1021 cgaacagggc ttcaaagtcc gctcctgcat ggattgcccc tgtaaaactg tggacgacat
     1081 aattcaatac ccatccgccg accacgctgt aaaacgacag caaaataaag caggcggcaa
     1141 cgcccatgcg cccgacccaa agccattgcg tgcccggacg cagagccctg aaggaatcga
     1201 cggcattttt accgcccgtg cgcccgatat aaaactcggc aagctggacg ggcagggcga
     1261 ccaagatagt aaatatcaaa aacagcagga aaaacaccgc gccgccgttg gtgcctgccg
     1321 tataagggaa tttccaaatc gcgcccaaac cgatggccga acctgccgca gcaaggacga
     1381 aaccgatttt ggacgaccaa gaagtatggt tactcataaa atttattaag aaatgtgaaa
     1441 g
//
LOCUS       DAXHPG010000247         1413 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXHPG010000247 DAXHPG010000000
VERSION     DAXHPG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.247"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(262..525)
                     /locus_tag="ACFM6F_002102"
     CDS             complement(262..525)
                     /locus_tag="ACFM6F_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165444.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(544..>1413)
                     /locus_tag="ACFM6F_002103"
     CDS             complement(544..>1413)
                     /locus_tag="ACFM6F_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6165445.1"
                     /translation="VHKIAHAIAGCAAAAANKGKCQDGAIGAVVGEIVGEALVKNTDF
                     SGMTASEIEKAKANITAYAKLVAGATVGVTGGNVDVAANASETAVKNNALDIIWDIGN
                     LVWDGGKWIYAKSIGDKQMAREAAIDFGVDAAAAAVPFVPAGATKISRGGAYVLKAGD
                     EAVDTAKAIQEIQKQTGIKLTYDKVNKVWTTPAGLDYGLDAKHGNRIKHVLAHTIPNP
                     NKPVHSVFNVSRKEVLPLVDEAWRMKGNPLPNDSSVYLVDMKKPIGTKGETKVRIVVQ
                     KGTNKIISAYPQK"
BASE COUNT          371 a          350 c          243 g          449 t
ORIGIN      
        1 ttggctgttt gggcgataac gcgtacgggc agtttggttt cgttcagctc gtttttgctg
       61 taattgcttt tacccacttt gatgccgatg aaacgggtac ttttctgaac attcaattgg
      121 tgttggtgaa tgccttcggc tgccagcagt tgcattgagg ccgtctgaaa aacggatatt
      181 cagacggcct attgctactt taaataatat ttgggtttaa aatatcatcg tatttcccaa
      241 attcggggaa cagaaaatca attatttaaa tttttcatcc tcataatatg ggtgatcaaa
      301 aagctctcct tcccacatat ttatttcttc tatgccttta tctttcataa actcatgata
      361 atcataaaaa tcttttccat attgtccgta aaaaattttc atatccccaa gccacatagc
      421 ataacattca tcgcataaaa tgacctcccc agagaaatat ttaggctttg catgataaag
      481 gtagccttgc tcacatcttg ggcaaatttt aatttcttta tccataagag attcctttct
      541 taattatttc tgaggatatg cagaaatgat tttatttgtt cctttttgca caacaatccg
      601 cacttttgtt tctccttttg ttccaatagg tttcttcata tctacaagat atacggatga
      661 atcatttggc aaaggatttc ctttcattct ccaagcttca tcaaccaaag gcaaaacttc
      721 tttacgggac acattaaaaa cagaatgaac aggtttgttt ggatttggaa ttgtatgggc
      781 taaaacatgt ttaatcctat taccatgctt agcatctaac ccataatcta accccgccgg
      841 tgttgtccaa accttattaa ccttatcata agtaagcttg attccggtct gcttctgaat
      901 ttcctgtatg gctttagccg tatcaactgc ttcgtctccc gccttcagaa cataagcccc
      961 gcctcggctg attttagtcg cacctgccgg aacaaaggga acggcagctg cggcggcatc
     1021 cacaccaaaa tcaatcgccg cttctcgagc catctgctta tcgccaatag atttggcgta
     1081 aatccattta ccgccgtccc atacgaggtt gccgatatcc caaataatat ctaatgcatt
     1141 atttttaaca gctgtttcgg aagcatttgc cgccacatca acattgcctc ctgtaacacc
     1201 tacagtcgct ccggctacca gttttgcata cgcagtaata ttcgctttag ctttttcaat
     1261 ttcagaagca gtcataccgc tgaaatcggt attcttaacc aaagcctccc cgacaatctc
     1321 ccccacgacc gcaccgatcg cgccatcctg acacttgccc ttattcgccg ccgctgccgc
     1381 acagcccgct atggcatggg caatcttgtg gac
//
LOCUS       DAXHPG010000248         1403 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXHPG010000248 DAXHPG010000000
VERSION     DAXHPG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.248"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(41..1033)
                     /gene="porB"
                     /locus_tag="ACFM6F_002104"
     CDS             complement(41..1033)
                     /gene="porB"
                     /locus_tag="ACFM6F_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HFC6165446.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVEHNGGQV
                     VSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVDDVKIEKYQIHRLVSG
                     YDNDALHASVAVQQQDAKLVEDNYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFD
                     DADLSNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVSTAGGVGLRHKF"
BASE COUNT          319 a          323 c          366 g          395 t
ORIGIN      
        1 gtctggcgac aggctttttg ttgataccaa tctttgcaga ttagaatttg tggcgcagac
       61 cgacaccgcc ggcagtcgat acgaatttgt tttcgccttt gccttcttgc aaccaaccgg
      121 cagaaaccaa ggcagaagtg cgtttggaga agtcgtattc cgcaccgaca accacttggt
      181 cgtaatcgtt gcttaagtct gcatcatcaa acgagccttt gaagccgtgg gcgtaagaaa
      241 cgcggggcgt tacgttgccg aagcggtatg ccaaggtagc ggcaacttcg gtttgagagt
      301 tgtgcgaata attgtcttca accaatttgg catcttgttg ctgtacggct acggaagcgt
      361 gcagggcatc attgtcgtaa ccgctgacca aacggtgaat ctggtatttt tcaatcttca
      421 cgtcatccac atcctgatgt cttttatagg caccgccata ttgcacgaag aagccgccgt
      481 ttttgtagtt gaagccggcg tggtaagatt cgctgttatg tctgcctgca ttgtcgttaa
      541 gcgcgtattg tacgctgccg ctgaggccgg caaattcggg agaatcgtag cgtacggaaa
      601 tgaggcgtgc ttcgggttcg gcaattttgt ttacacccaa atagtcgctt ttgctatccc
      661 aaggattgat gtcgccggtg tctttcagga cgctgttcaa acggccgacg cgcaatttac
      721 cgaagccgcc tttcaaaccg atgaaggatt ggcggttgcc ccaaccggag tcagtgccgg
      781 cgatagatgc cttttgctca acctgccaaa tggctttcag accgttgccg aggtcttctt
      841 ggcctttgaa gccgattttc gaacccaaat caacgatgcc ggtaccggtt tcaacgctaa
      901 ccacctgacc tccattgtgc tctacagagc gggaagtttc tacgccggct ttgatggtgc
      961 cgtacagggt aacgtcagcc attgctgcaa caggaagggc tgccaaagtc agggcaatca
     1021 gggatttttt cattgctgta ttcctttttt ggttaagaaa tttaagcagg ccgggctttc
     1081 caagccgctt agctttgcat ttaccgccga cgttttcggc gattccatgt ctgaaactat
     1141 agatgttcct gtctgaaaaa acaaacttct gcctgttttt gtgtggaatt cgatgtgtat
     1201 tttgaagggc ggatagagat attgtgggta ttttttgttt tataacatat ggttatttaa
     1261 attttttaag atttgcattt ttacaacact tactcgggag ggtattggag ggcattgcaa
     1321 accgggggtt ataaagacgg caaaaaacgc gcacgtggtt tctttcggaa cgggcggatg
     1381 caaaacccgc ccctgcggca gga
//
LOCUS       DAXHPG010000249         1378 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXHPG010000249 DAXHPG010000000
VERSION     DAXHPG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.249"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..435
                     /locus_tag="ACFM6F_002105"
     CDS             <1..435
                     /locus_tag="ACFM6F_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6165447.1"
                     /translation="KQAIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQT
                     GQPVSAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMR
                     TVDVGKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            442..951
                     /locus_tag="ACFM6F_002106"
     CDS             442..951
                     /locus_tag="ACFM6F_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165448.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
     gene            1020..>1378
                     /locus_tag="ACFM6F_002107"
     CDS             1020..>1378
                     /locus_tag="ACFM6F_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165449.1"
                     /translation="MIYGSMPSEKLTIFQTAFVMQVNIQIPCMLYRRGSVKPPLFEAP
                     RLLPSFTDPVVPKLSAPGGYIVDIPKGNLKTEIEKLSKQPEYAYLKQLQVAKNINWNQ
                     VQLAYDRWDYKQEGLTEA"
BASE COUNT          469 a          266 c          249 g          394 t
ORIGIN      
        1 aagcaggcta taccaaccca gacagttaaa gaacttgatg gcttactaca agaatcaaaa
       61 aatataggtg ctgtaaatac acgaattaat atagcgaata gtactactcg atatacacca
      121 atgagacaaa cgggacaacc ggtatctgct ggctttgagc atgttcttga ggggcacttc
      181 cataggccta ttgcgaataa ccgttcagtt tttaccatct ccccaaatga attgaaggtt
      241 atacttcaaa gtaataaagt agtttcttct cccgtatcga tgactcctga tggccaatat
      301 atgcggactg tcgatgtagg aaaagttatt ggtactactt ctattaaaga aggtggacaa
      361 cccacaacta caattaaagt atttacagat aagtcaggaa atttgattac tacataccca
      421 gtaaaaggaa actaactaaa tatgagtaac tttgaaaaaa aatatatttt agaattaaat
      481 gatgctttaa gccatttaaa tcataactct acctcatttg atttattgaa agttttgatt
      541 tcatggttat caaacgatat tgtcattgat aaatttaaaa ttttaggtta tgactttagt
      601 aaatatatcg aaatgaatcc cgatgactat ccggttgaaa aatctatatt gaatagagag
      661 gaaattattt atctcaaaaa caatatttat cgtaaaatat catcaggaaa ttttaaattt
      721 caatactttg tacaatatat tagagatatt ttagaatatt tatttattga acatattgaa
      781 agagtctgtc cttactgcga atggggtgaa atgcaaaaat tagaagaaca aaatacgcat
      841 gaaacggtgt atctctgtac tcaatgtgga tgtgcttttt ataacgataa ttcacaattt
      901 ttattaaaaa cccctttaac cattccaatg aaacgtgatg aatttaaata aacaagccgt
      961 agcctgcatg aaccctaaaa tccacgtgta gcgtgtgtgc gccagcacgc atgcgttcca
     1021 tgatttacgg ctcaatgccg tctgaaaagc tcacaatttt tcagacggca tttgttatgc
     1081 aagtaaatat tcagattccc tgtatgctgt acagacgcgg gagtgttaag ccccccttgt
     1141 ttgaagctcc gcggctcctg ccgagcttca ccgaccccgt tgtgcccaag ctctctgctc
     1201 ccggcggcta cattgtcgac atccccaaag gcaatctgaa aaccgaaatc gaaaagctgt
     1261 ccaaacagcc cgagtatgcc tatctgaaac agctccaagt agcgaaaaac atcaactgga
     1321 atcaggtgca gcttgcttac gacagatggg actacaaaca ggagggctta accgaagc
//
LOCUS       DAXHPG010000250         1358 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXHPG010000250 DAXHPG010000000
VERSION     DAXHPG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.250"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            128..1258
                     /locus_tag="ACFM6F_002108"
     CDS             128..1258
                     /locus_tag="ACFM6F_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6165450.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     TIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRLLKEKGIDE
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGGDLERLAARDIIRFPGFVNNVSEVIKEH
                     HIFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAE
                     KMIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          373 a          324 c          318 g          343 t
ORIGIN      
        1 ggcaacgccg taccggtttt tgttaatcca ctataatacg caggcaaacc caagccttgc
       61 tgggttacag gatgaaaccc tgccgtagca gaccgcattt ccaatccata ttcaaggtgt
      121 ccgccttatg aaaatcgttt ttatcacaac agtcgcatcc agcatttacg gtttccgcgc
      181 ccccgtcatt aaaaaattaa tcggcaaaaa ccatcaggtg tatgcctttg tatcggagtt
      241 ttccgacaat gaattggata ctatcaggga aatgggggta acacccgtta cctaccgttc
      301 aaaccgcagc gggctgaacc cgttttccga tataaaatcc accttcctca tctttaaaga
      361 actcaaaaaa atatcgccgg atttggtttt cccttatttc gcaaaacccg tgattttcgg
      421 cacttttgcc gcaaaactgg caggcgtgcc cagaatcgtc gggatgctgg aaggtttggg
      481 attcgcattt accccgcagc cggaaggcat accgttaaaa acaaaaatca taaaggggat
      541 tttgattgcc ttgtaccgca ttgccctgcc gatgttggaa agcctgattg tattaaaccc
      601 cgacgacaaa gacgaactga cggacaaata cggcatcaaa ataaaaaaca tccatatttt
      661 gggcggaatc ggtctggatt tgcggcaata tccttattcc gaggcggata ttcccgatga
      721 aaaagaaccc gtaaaatttc tctttatcgg cagattgttg aaagaaaaag ggattgatga
      781 gtttattcgg gcggcggaac aggttaagga caaatacccc gatacggttt ttaccgcttt
      841 gggcgcaatc gacaaatcac gcgggggggg gggcgatttg gaacgccttg ccgcccgcga
      901 tattatccgt ttccccggtt ttgtgaacaa tgtttccgaa gtgataaaag aacatcatat
      961 attcgtattg ccgtcttatt atagggaagg cgttccccga agcactcagg aggcaatggc
     1021 cgtcggcagg gcagtgatta cgacggatgt ccccggatgc agggaaacgg tcgccgacaa
     1081 ggtcaacggc ttcctgatcg aaccttggaa tccccgcatc ttggccgaaa aaatgattta
     1141 ttttatcgaa aacagggaag ccgttcgcct gatggggaat gcaagttatg cgattgccaa
     1201 agataaattc gatgccgaaa aagtcgattt aaaattactc gatattttga aggcgtaaac
     1261 aaggctgccc gcttttgagt ttcggtcatt tctgataaac ctccgtcatt cccgccacac
     1321 ccccgtcatt ccctcgaaag cgggaattca ggttcgtt
//
LOCUS       DAXHPG010000251         1332 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXHPG010000251 DAXHPG010000000
VERSION     DAXHPG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.251"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            544..1053
                     /locus_tag="ACFM6F_002109"
     CDS             544..1053
                     /locus_tag="ACFM6F_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6165451.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNHGIWPANNNSAGVASTATDIKGKYVQSVEVKNGVVTATMASS
                     NVNNEIKSKKLSLWAKRQNGSVKWFCGQPVTRTTATATDVAAVKADATANINTKHLPS
                     TCRDASDAS"
BASE COUNT          408 a          388 c          258 g          278 t
ORIGIN      
        1 ccgacgaggc tggattcccg cctgctcggg aatgacgaag ccatccgcac ggaaacctgc
       61 cgcgatgatt caactgccgt ttgcacagga acaccacgcg ccgatttcaa tcactttcca
      121 agaaaacgga aatttttaaa aattaaattt aaaatttccc acccaaccca ccctatgcta
      181 ccgcgcaaat tcaaaaatct caaattccga cccaatcaac acacccgatg ccccatgcca
      241 atagaaatac cccacgccaa taaaaaagtg ccgaaaatcg gcactaaaac tgacaatttt
      301 caacactcct gacaaaaaac ggtaaccccc tgcttcctca aaccacaccc aaacgaaaga
      361 aaatgtaaaa taaaaacaac catataaaga taaacgcata aattttcacc tcaaaacaca
      421 aacttgatac caatcttgct ttataataca caggtatcgc aacaaaatac cgatggtcaa
      481 atacattgca taatgccgat ggcgtaagct tgaggcattt cctttccaat taggagtaat
      541 tttatgaaca cccttcaaaa aggttttacc cttatcgagc tgatgattgt gattgccatc
      601 gtcggcattt tggcggcagt cgcccttcct gcttatcaag actacacagc ccgcgcacaa
      661 gtttccgaag ccatcctttt ggccgaaggt caaaaatcag ccgtcaccga gtattacctg
      721 aatcacggca tatggcccgc caacaacaat tctgccggcg tggcatccac cgccaccgac
      781 atcaaaggca aatatgttca aagcgttgaa gtcaaaaacg gcgtcgttac cgccacaatg
      841 gcttcaagca acgtaaacaa cgaaatcaaa agcaaaaaac tctccctgtg ggccaagcgt
      901 caaaacggtt cggtcaaatg gttctgcgga cagccggtta cgcgcaccac cgccaccgcc
      961 accgacgtcg ccgccgtcaa agccgacgcc accgccaaca tcaacaccaa gcacctgccg
     1021 tcaacctgcc gcgacgcaag tgatgccagc taaggcaaat tataccataa attttaaata
     1081 aatcaagcgg taagtgattt tccacggcca ctcggattaa tccgggtggc ttccttttta
     1141 agggtttgca aggcaagcgg gggcgtccgt tttggctgaa aataatatat cgattgcgct
     1201 tcaaggctct gcgcgtacct cattgccgcc cgtttaaaca cggggtttat ctgacaggcg
     1261 cgcaatccgc cctctcattt accgaacaag cggactggat tcccgcctgc gcgggaatga
     1321 cggcaaccgg cc
//
LOCUS       DAXHPG010000252         1317 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXHPG010000252 DAXHPG010000000
VERSION     DAXHPG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.252"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(108..1031)
                     /gene="lpxC"
                     /locus_tag="ACFM6F_002110"
     CDS             complement(108..1031)
                     /gene="lpxC"
                     /locus_tag="ACFM6F_002110"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC6165452.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPAFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          349 a          314 c          333 g          321 t
ORIGIN      
        1 taggttcgcg ggaatgacga attttaggtt tctgattttg gttttctgtt tttgtaggaa
       61 tgatgaaaat ttaagtttta ggaatttacc ggaaaaaaca gaaagcgtta tccacaagtt
      121 ctgatgttca gctcgtgaaa tgcgtcgggc aaatcatcgc tgtcggcaaa ttccacccgg
      181 tcgtaagccg tttcgtctgc caaaaccgcg cgcaagagtg cgttgttgat ggcgtgtcct
      241 gatttgtagc cttcaaacgc gccgacaatc ggatgtccga cgatatacaa atcaccgatg
      301 gcatcaagga ttttgtggcg cacaaactca tcgggatagc gcaagccttc aggattcagg
      361 acatccgtgt cgtcaatcac gatggcgttg ttcaaattgc cgcccaaacc cagattgtgg
      421 gcgcgcatca tttccacttc gtgcataaag ccgaaagtgc gagcgcgcgc gatttcgtcg
      481 atgtaggatt tgccggcgaa atcgatttca aaagtgggcg agctgcggtt gaaagccgga
      541 tggtcgaatt cgatggtcag cgttacctta aagccgtcat acggcgtaaa gcgcacccat
      601 ttgcccgctt ctttgatttc gacaggcttg aggattttca aaaaacgctt ttgcgccttt
      661 tgatcgacca cgcccgcatc ttgcaaaagg taaataaacg gcaggctgga gccgtccata
      721 atcgggattt cgggtgcgtt cagctcaatc agcgcgttgt cgataccgta ggcggacagc
      781 gcggacataa tgtgttcgat cgtgccgacg cgcacgcctt tgtcggtaac gatggtggag
      841 gaaaggcggg tatcgttgat caaataaggg gtcagcttga tttgttcgcc catctcgccg
      901 tccaaatcgg tacggcggaa ggaaatcccg ctgttttcag gcgcggggtg cagggtcagt
      961 gcgacccgtt cgcccgaatg cagcccgact ccggtaacgc tgatggattt cgccaaagtt
     1021 ctttgcagca taaaccgctt ccttatcaat ggggtaagtt ttggaataat acgataaaac
     1081 cggaaaaaca ggctatgttt ttccatagta tttgccaatg tatccgtttt caatacgtaa
     1141 gccgcataaa aatgtatagt ggattaacaa aaatcaagac aaggcgacga acccgccccc
     1201 tcctgaaaaa cgcaaaaaat gccgtccgaa aacctttcgg acggcatttt cgcgtgaacc
     1261 gtcattccca caaggacata aaaccaaaac agaaaaccca aaacagcaac ctaaaat
//
LOCUS       DAXHPG010000253         1230 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXHPG010000253 DAXHPG010000000
VERSION     DAXHPG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.253"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..447)
                     /locus_tag="ACFM6F_002111"
     CDS             complement(<1..447)
                     /locus_tag="ACFM6F_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6165453.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAVLFY
                     RKIRTVINYHLVLAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRG
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIHYR"
     gene            complement(567..1025)
                     /locus_tag="ACFM6F_002112"
     CDS             complement(567..1025)
                     /locus_tag="ACFM6F_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165454.1"
                     /translation="MPSEHAFGRRFVGGYRSGTSEKGFQAVFGRVVTVENVIRLPEKF
                     GRFSGGLWYWAQQTQVQIRRCAVIVRMPIQPKQTSVFRKWMFDGAKVVRRMEGNLWVF
                     GMVALEKTCFKGQMPSENRFQTAFSVYLKQTGKGSNILQSSYSHKRFIVN"
     gene            1046..1230
                     /locus_tag="ACFM6F_002113"
                     /pseudo
     CDS             1046..1230
                     /locus_tag="ACFM6F_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Caudovirus prohead protease"
BASE COUNT          313 a          329 c          247 g          341 t
ORIGIN      
        1 gcggtaatgg ataaaaccgc tcacgtccaa cttgtcgcag ctgctcagac tatcggtata
       61 aacaatactg tccggcatga ttttcttttt gatgacaggg agtaacgttt cagacttggc
      121 attatctacg acaacggtat agccccgtcc gttgcgtttc agaatgccga agacaaccac
      181 ttttcctgcc gcaccgcgac cacgtctgcc tttacgccgt ccgccgaaat cgctttcgtc
      241 cggctcgaca gggccctcaa aaacctcatc ggcagccaag accaaatgat agttgataac
      301 cgtgcggatt ttgcggtaga acagtactgc cgaattggga tggataccca aaatatcggc
      361 ggcagaacgg gcggtaactt ccagtacaaa aaaacggagc agttctttct gtactttttt
      421 ctttaatttg cagtgcgtta tcttcatatt tcgagggtaa catatctgct aatctagtac
      481 agcccctagt ttaaagcttt gtttcttaag tccgcagagt atgccatggt tagaccttca
      541 aagttgagta ttgtactatt ttgtttttaa ttaactataa aacgcttgtg tgaataggaa
      601 gactgcagaa tattgctgcc ttttcctgtt tgctttaaat aaacagaaaa tgccgtctga
      661 aaccgatttt cagacggcat ttgtccctta aaacacgttt tttcaagcgc gaccatacca
      721 aacacccaca ggttgccttc catccttcgt acaacctttg ctccatcaaa catccacttc
      781 ctaaatactg atgtctgctt aggttgaatc ggcattcgta cgattacggc acaccgccta
      841 atctgtacct gcgtctgttg cgcccaatac caaaggccac ctgaaaatct cccaaacttt
      901 tcaggtagcc ttatcacgtt ttcgactgtt accacacgcc caaagaccgc ctgaaaccct
      961 ttttcagacg ttccgctccg atacccccca acaaaacgcc gcccgaacgc atgttcagac
     1021 ggcattttct ttgcgtcgat tcattgcggt gtctacctgc tttaaggcgg acaaaggtgc
     1081 ggaagcggta aacaacgata cagaacataa tgctacatat tttagccatt tcccttccaa
     1141 acaaaaaagc accgacggcg gccgatgccc cttcccttac aggttcccct attttttcac
     1201 tgcggtcgtt gccggtttgg cgggggcttt
//
LOCUS       DAXHPG010000254         1217 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXHPG010000254 DAXHPG010000000
VERSION     DAXHPG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.254"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(129..1136)
                     /locus_tag="ACFM6F_002114"
     CDS             complement(129..1136)
                     /locus_tag="ACFM6F_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6165455.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPASDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYIPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNMLSIGQIVKVKPAGYAAPKTAAVE
                     SRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGI
                     VWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHN
                     SSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIA
                     F"
BASE COUNT          281 a          327 c          347 g          262 t
ORIGIN      
        1 ccaaacacag tcaaacaaat ttatatgccc cgtcccttct gaatgatttg aaaacacagc
       61 cgccaaaaac aaaaatgccg tctgaaaacc tttcagacgg catttccaat ttgatttcag
      121 gcagaaagtc agaacgcgat atagctgttc gggttaaccg gtttgccgtt ttgccgcacc
      181 tcgaaatgaa gctgcgttct ggaagcgtcg gtattgccca tcaaagcgac ctgctgcccg
      241 cgtttgacct gctggccttc gccgaccagc aatttttggt tgtgcccgta tgcggtcagg
      301 aaggaagaat tatgctggat gatgaccaga ttgccgtatc ccctcaaacc ggaacctgca
      361 taaaccactt tgccgtcagc cgccgccaaa acgggctgtc ccgcattacc ggcaatatcg
      421 acacccttgt tgttgccgcc gaaatcggca accactttac cttgcgtcgg acgctgccaa
      481 acaatgccgc cgaccgaacg cgtgccggaa ggcgaagcgg caggagattg cggggcgggc
      541 gcgggaaccg ctttattttc cgcagcgggc gcggcaggtt gcggcgcgga ctgcacaggc
      601 ggttgcgcgg cgggtttcac aggggtttgc acggcagccg gtacggcggg cctgctttct
      661 acggctgcgg ttttcggtgc ggcatatcct gccggtttga ctttaacaat ctgaccgatg
      721 ctcaacatat tgtcggtcat gccgttccac gcacggaaat cgtcttgaga gatatggtag
      781 cgtttggaaa tgttgtacac cgtgtcgccg cgcacaatag tatgcgtcgc cgcgttgatg
      841 tcgacgggtg cgtaagaagg aatgtatgta cccgaaacgg caggtgcaga cggcggaaca
      901 taagcaggag gcgtataaac cggcgcgctt tgcaccggcg gcacataagg cgcatcgctg
      961 gcaggagccg ggctgtacgg cgttgctcca taggggttgt tgtaaactgc cgaagacggc
     1021 gcgtcctgca tacctgaatt gcctgcaatg acgggggcag gctgctgggt agcacaaccg
     1081 cccaacagtg cggcaaagga ggcgcaagcc gccaaaagtg tcgtttgttt caacataaga
     1141 taaccttcat gttccgatat atagcctgaa tgcggtatat cataataaaa atgcgcgttc
     1201 ttctcaagcg caaagcc
//
LOCUS       DAXHPG010000255         1128 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXHPG010000255 DAXHPG010000000
VERSION     DAXHPG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.255"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(231..1085)
                     /locus_tag="ACFM6F_002115"
     CDS             complement(231..1085)
                     /locus_tag="ACFM6F_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6165456.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWNVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          312 a          336 c          296 g          184 t
ORIGIN      
        1 tcccgtcatt cccgcgcagg cgggaatctg gacatccaac gctgcagcaa tttgtcggaa
       61 ataaccgaaa ctcaaaaaac cggatgcccg cctgcgcggg aatgacgatg caaagatggt
      121 cgttatttcg gataaatatc cgtcttttcg ttccggatac ccgctttcgc aggaatggag
      181 atggaaagat ttcagcctat tcggcaatca tcaaataccg cttcccaact ttatttttga
      241 tgcgacagcc ggccggcaac caaggtggcg gcgatgacga caaacacgcc caaggcggac
      301 acgggcgaca ggtgttcgcc caaaatcaaa accgccagca gcacgccgac gacgggttcg
      361 agcgaaatca acagtcccga aacgttggca ggaacacggc tcatcccctt gttccacagc
      421 caataggcgt accagccgca ccccaagccc aaatacagca gcgacaatac catcccgacg
      481 ttccagtcca cggtataact ttgcgccaaa gcaagcgaaa acggcaggca catcaacgat
      541 gcggcggcaa tggaaacaga tgtgaatgcc ggtgcgccga tgcgtgcaat cagcctttgc
      601 gtcggacgca tagcggcaca aaagcccgcg cccgccaaca acaccagcag gcagccgaac
      661 cagccgactt cgccgccctc ttccgcaccg cccgccatca gcagcgcgac accggcaaat
      721 gccgccgcgc cgcatatcca gtggtaggca cgcgctttgt cgttgaagaa aaagtgtccg
      781 acaaacacca tcagcagtgg ctcgagtccg acaatgaccg atgcgctggc ggcggaagtg
      841 tatttcaacc cgacaaactg aagcagcagg gtcagcacat agttgacgaa cgacacaatc
      901 agcaacggct tccattcctc acgcggaatc ttgccgacat gacggcggca ggcgggcagt
      961 gcaggcagcg cagcaatcag caggcgcacg ccgaccatca atgcgggatc gatgccgcca
     1021 tagacatatt tggcggcaat aaacgagctg ctccagataa tcagggcaag gatttggtaa
     1081 aacatagtga gtgataaagt cagatggtca tacaaaagta tagcggat
//
LOCUS       DAXHPG010000256         1111 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXHPG010000256 DAXHPG010000000
VERSION     DAXHPG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.256"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..1022)
                     /gene="mafB"
                     /locus_tag="ACFM6F_002116"
     CDS             complement(<1..1022)
                     /gene="mafB"
                     /locus_tag="ACFM6F_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6165457.1"
                     /translation="MKLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAATTVWGGKKV
                     ELNPTK"
BASE COUNT          260 a          272 c          252 g          327 t
ORIGIN      
        1 catttggtcg ggttaagttc tactttttta ccgccccaaa ccgtagttgc ggcctctgct
       61 acggaaaggg cagtttgggc agtagctgta atgttcggat gggcatcagc ccattgtctg
      121 gcagagttga tactgtcttt taccgcaaaa gcactgtctt gtaatgcggt tgcggccgca
      181 agttgtgctt ccggacttaa atttcctaaa ttgtgaatac cctgtaaagt tttccgtgct
      241 gccgcatagg ttaccgggtt taccgcactg tctgtaattg ccccaactcc caatccctga
      301 aaacccatta aaaaaggatt aaccgcacct tggacgatgc cgcgaatatc atccatacgg
      361 ttagcccacc aatttttatt ggggtcgctt tcccatatca gttttcctgc ttcatccgca
      421 cggctgaaaa aaccagaggc ggcaccggca ttttctttta gccaacggtc tgaaaatggg
      481 gcttgaggga caatattagt ctttgttttt gttgaagttc ctttgacata atagctgtat
      541 atatcccttg ctcctccggg gggcggataa tcgctgcctt gcggcccgtc atatccatcc
      601 tccggatgaa tttccgaccc tgtccgatga agttggtaaa cagtaaaacc accgtctaca
      661 ccgccgctga aatcagaagt gctttttgaa tcatgattat cgaacggact gtgtacttca
      721 tgtccatgtc ctgaaaaatg ggtttcataa ccgataatgc cttcaaaccc tgtccgttcg
      781 tgtgtaatag gcagtatggg gccgaccgca gttgcatcaa atgtctggac ggcgtaaacc
      841 cgatttttaa cactgccgcg agcattacca aacagatggt atttaccacc cggttcgtag
      901 tgttttgcct gcatatcatc gcgtaaacgg gcatccaaac cgttcgcatg actaatgggg
      961 atttgcagca acgatattgc cgctgcaaac agcatcatga atttttgaat aggcaatttc
     1021 attttgtttt ccttggtagc aagcggttat ggtcgtgtga atcaaggttg cccttgtcta
     1081 tgttgtcgca ctacttcatc gctgtatcca t
//
LOCUS       DAXHPG010000257         1082 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXHPG010000257 DAXHPG010000000
VERSION     DAXHPG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.257"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            284..721
                     /locus_tag="ACFM6F_002117"
     CDS             284..721
                     /locus_tag="ACFM6F_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapD"
                     /protein_id="HFC6165458.1"
                     /translation="MSRYLITFDMDTNCLKDNYHGNNYTNAYSDIKTILARHGFENIQ
                     GSVYLGREGISEAHGTIAIQELTARFDWFYSCISNIKFYRLESDLNAQFIADGVYQAK
                     QAFLQRVEQLRISLTEAGLSDEQINQVLEKQKFELESPNLKLN"
BASE COUNT          338 a          248 c          219 g          277 t
ORIGIN      
        1 gccaccaaaa cccttgctag ctaagggtta aacagctcac ttgaaatcta cttaagtcta
       61 atctaaacta tccaatatgg atagattttt aaacataggg caagcagcaa aattattgta
      121 gctgaaagca caatcactcg ctggtggtct caaacacgtg ccgactacct cgccgaaaac
      181 actatcagcc gcgataaacc gtgggaaaag ctcgttatca gccgccgcac ttggtactat
      241 cgcgggaaac cgatgctgtc tgaaacgcaa caggagaaaa ataatgagcc gttacctgat
      301 tacctttgat atggatacca actgcctgaa agacaattac cacggaaata actataccaa
      361 tgcctactcc gatattaaaa ccatcttggc tagacatgga tttgagaaca ttcagggcag
      421 tgtttatcta ggccgtgaag gcatcagtga agcacacgga acaatagcca ttcaggaact
      481 gaccgctcgg tttgattggt tttactcctg tatttcaaac attaagtttt accgccttga
      541 aagtgatttg aacgcacaat ttatcgctga tggtgtgtat caagccaaac aggctttcct
      601 tcaacgtgtt gaacaacttc gtatatccct aacagaagct ggattgtctg atgagcaaat
      661 caatcaggtt ctggaaaaac agaaatttga attggaaagt cctaacctga aattaaatta
      721 acctccttta ctcaccaaca tccgccgcag ctctgtcagt ttttggcgcg ctgcggcgat
      781 ttctgtgcgt tttagagctt cgggtagggt gtgaaacaac tcactcgaaa tttacttaag
      841 tctaatctaa actatccaag cagtaattag tacaaaaaag gcaaacttat tttaggagct
      901 taaaattgca gccgcgataa accgtgggag agtctcggca tttcccgcgc cacttggtac
      961 aaacgtggca aaccgatgcc gtctgaaacc gtacaacagg agacaaaata atgcttgctc
     1021 aaatcgaact cacaccctgc cagctaatgg tttacgtaac ttaaggttac tggatttacg
     1081 ca
//
LOCUS       DAXHPG010000258         1073 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXHPG010000258 DAXHPG010000000
VERSION     DAXHPG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.258"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..344)
                     /locus_tag="ACFM6F_002118"
     CDS             complement(<1..344)
                     /locus_tag="ACFM6F_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165459.1"
                     /translation="MHTQRKNFSDSISLHDCYCTEIKVEGRNVCFNFEDGFVVPDNNP
                     NNQAGKHLKTDFSRVVLTHKNENAEDDYLFDIFEDIVFLGKRLFTVRKFLDFSELLEM
                     VNTQGYFLEFLY"
     gene            442..>1073
                     /locus_tag="ACFM6F_002119"
     CDS             442..>1073
                     /locus_tag="ACFM6F_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6165460.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLEQVIDWQPIEHYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKLINRQLTEKGLKIEKASAAVIDATIIQTAGSKQRQAIE
                     VDEEGQVSGQTTPSKDKDARWTKKNGLYRLGYKQHTRTDEEGYIKKLHITP"
BASE COUNT          342 a          294 c          193 g          244 t
ORIGIN      
        1 aaatacagaa actcaagaaa ataaccttgt gtattgacca tctcaagcaa ttcagaaaaa
       61 tcaagaaatt ttctgaccgt aaacaaacgt ttccctaaaa aaacgatgtc ttcaaaaata
      121 tcgaacaaat aatcatcttc ggcgttttca tttttatggg ttaaaacaac acgggaaaaa
      181 tctgttttca gatgcttgcc cgcttgattg ttcggattat tgtccggaac gacaaaaccg
      241 tcctcaaaat taaagcagac gttgcgtcct tctaccttta tctctgtgca ataacaatca
      301 tgtagagaaa tgctatccga aaaatttttt ctttgtgtat gcaaaaaaag ttttcattca
      361 agtacccata tctaacacga gacctttgca aaattcccca aaatccccta aattcccacc
      421 aagacattta ggggattttc catgagcacc ttcttccggc aaaccgcaca agccatgatt
      481 gccaaacaca tcgaccgttt cccactattg aagttggagc aggtgattga ttggcagccg
      541 atcgaacact acctgaaccg tcaaaaaacc cgttacctta gagaccaccg cggccgtcct
      601 gcctatcccc tgctgtccat gttcaaagcc gtcctgctcg gacaatggca cagcctctcc
      661 gatcccgaac tcgaacacag cctcattacc cgcatcgact tcaacctgtt ttgccgtttc
      721 gacgaactga gcatccccga ttacagcacc ttatgccgct accgcaactg gctggcgcaa
      781 gacgacaccc tgtccgaatt gctcaaactg attaaccgcc aactgaccga aaaaggtcta
      841 aaaatagaga aagcatccgc cgccgtcatt gacgccacca ttattcagac cgccggcagc
      901 aaacagcgcc aggccataga agtcgacgag gaaggacaag tcagcggcca aaccacaccg
      961 agtaaagata aagatgcccg ttggacaaag aaaaacggtt tatacagact cggttacaaa
     1021 caacataccc gtaccgatga ggaaggctat atcaagaaac tgcacatcac ccc
//
LOCUS       DAXHPG010000259         1051 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXHPG010000259 DAXHPG010000000
VERSION     DAXHPG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.259"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..165
                     /locus_tag="ACFM6F_002120"
     CDS             <1..165
                     /locus_tag="ACFM6F_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6165461.1"
                     /translation="TKQVTKGVFDGKGEAKIPSYTWHHHQDTGRMQLISERKHTQTGH
                     IGGEAMDKGK"
     gene            168..566
                     /locus_tag="ACFM6F_002121"
     CDS             168..566
                     /locus_tag="ACFM6F_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165462.1"
                     /translation="MLGLTRNNCDDLGFAILCYERNAADKKEFLDWLYEIVRTVPAES
                     FPRYLIDLIDCLESDDCSINIYNIIGFVPTTGMTKKEKDAVYGITYIRNQEFYDIPIT
                     REKALKALKKHPEIYQKFRHFFPFVELPPL"
     gene            667..>1051
                     /locus_tag="ACFM6F_002122"
     CDS             667..>1051
                     /locus_tag="ACFM6F_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165463.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDVGIGDIGNIVGFAPSSSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPP"
BASE COUNT          346 a          171 c          229 g          305 t
ORIGIN      
        1 acaaagcagg taaccaaagg agtgtttgac ggaaaaggag aagctaaaat accgagctat
       61 acttggcatc atcatcagga tacgggacga atgcagttaa tatccgaaag aaagcatacg
      121 caaacagggc atataggtgg tgaagcaatg gataaaggaa aatgattatg ttggggctta
      181 cacggaacaa ttgtgatgat ttgggatttg ctatattgtg ttatgagcgc aatgcagccg
      241 acaaaaaaga attcttggat tggttatatg aaattgtcag gactgtccct gccgaaagtt
      301 ttccaaggta tctgattgat ttaatagatt gtttggaatc agatgattgt tccataaata
      361 tatataatat tattggcttt gttccaacta ccggtatgac gaaaaaggaa aaagatgccg
      421 tatatggcat tacctatata cggaatcaag agttttacga tattccgatt actagggaga
      481 aggcgttaaa agccttaaaa aaacatcctg aaatttatca gaagtttcgg catttctttc
      541 cgtttgtaga actgcccccg ctttaaacag tcaaaatgcc gtctgaaacg atattcggct
      601 ttcagacggc atttttgaca caaagcaggt aaccaaaaga gcgtttgacg gcaaaggaag
      661 ataattatgt ggaaaatcat aaaagaggat agtgatgatt taggatttgc aattaaatgc
      721 ttattctctc aatctattga tttaaatgaa ttcaagttat ggattgaaca agtaatacga
      781 gatatgccca ttgaggacat ccctttttat atttttgact tggcggattt cgatgtagga
      841 attggcgata ttggcaatat tgtaggtttt gctccgagtt ccagcctgcc gaaatcgaaa
      901 aaaaatgcct tgaccggcat tgccttctta agggggatag atgtctatga ccctcccgtt
      961 tcaaaagaaa aagcattaaa agccttagag aaatatcctg aaatttatca gaaatttcag
     1021 catttctttc cgtttgtaga gctgcctccg c
//
LOCUS       DAXHPG010000260         1035 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXHPG010000260 DAXHPG010000000
VERSION     DAXHPG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.260"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(302..688)
                     /locus_tag="ACFM6F_002123"
     CDS             complement(302..688)
                     /locus_tag="ACFM6F_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC6165464.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGTSARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(685..930)
                     /locus_tag="ACFM6F_002124"
     CDS             complement(685..930)
                     /locus_tag="ACFM6F_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFC6165465.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLTGIEKP
                     ARNAGRLKKFSHMGNTDISRILEDDNETAALFEESAL"
BASE COUNT          281 a          244 c          184 g          326 t
ORIGIN      
        1 acacagccat atatattaag tacctgctct ttatgcgctt ttttaggaaa ggaagatcaa
       61 acggttcaat agcgaagtct tgcccaaatt tgatggacag gatatagaat acatgcgtgc
      121 ataccacacc ctgccaaagt tgggagatgc acttaaggtt gcacttgtgg tcgccaacta
      181 tgcctataca cgcggatttc ccgcgtttat aggccgcgac ggaagtctgg cttttaagcg
      241 aagacaataa ccgaaaagac cgcctcggcg gtctttcttg tgtacacagt ttttatattt
      301 gtcagcttga tgcattgaca actctaattc catattgcgg aatatattca tcgacagtca
      361 tcagttcaaa gccttccgct tgggtttgtg caatcaacat cctatcgaaa gggtctttgt
      421 gtatttccgg aaggcttcca gcctgttttg catgaaacag acctataggc agcatttcaa
      481 aatcctcttc ttgaagcaca tcaaaaaact cttccggtaa tttcaacaac cccttgttct
      541 gcttgatgga aatttcccaa atacttgctg cactgacaaa gatcgcattt ctcggatttt
      601 ctatcagttt gcgtgcagat gtccccagtt tcttgtcatc caacaaccac cacagcaaag
      661 catgggtatc aagcagaatc tttctcacag agccgactcc tcaaataaag ctgccgtttc
      721 attgtcatcc tcaagaatac gtgaaatatc cgtatttccc atatgactga attttttcaa
      781 ccttcctgca tttcgtgccg gtttttcaat accggttagt tggacgcaag gcttacctgc
      841 tttcgcaata ataacgattt cccctgcttc tgctctttga atcaattgac tcaaattggt
      901 ttttgcctga tgaatatttg cttgaaacat aacacttctc atgattagct aacttaacta
      961 atatacatca ttaccaagat tttgggaatc tcattacata tatttgatta tatccgccgt
     1021 tttattcaca ccttg
//
LOCUS       DAXHPG010000261         1020 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXHPG010000261 DAXHPG010000000
VERSION     DAXHPG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.261"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(49..987)
                     /locus_tag="ACFM6F_002125"
     CDS             complement(49..987)
                     /locus_tag="ACFM6F_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165466.1"
                     /translation="MDAYTEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT          188 a          287 c          320 g          225 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat aggtcgtctg aaaacgggct aacggctgcc
       61 cagcggcaat ccgatgtccg aaatcagcct gtcgggcagg acgtcgtcgc ggaagcggca
      121 aagctgcgcg ggacggcctt tgctgcccga tacgccggta tccgacggct cgataaggtt
      181 ttgctgctga atctggcggc ggaagttttg cttgtgcagc aatcttccgc tgatggcttc
      241 gacactgttt tgcagttgca gcagcgtgaa ttcgggcggc atcagttcaa aaatcacggg
      301 gcggtatttg attttggcgc gcaggcggga cagggcggtc gccagtacgc gacggtgatc
      361 gtggcgcatg ggctgccccg tgagcgcgaa gtcgaagttt gcctgcggct cggcggcttc
      421 cactatcaga ccgctttcat acagcatttc atagcgttgt aaaacgtatt cttccgacca
      481 gttttccggt tcgacccccc agcacaaatg aatgcgcttg agccgcattt gacgcacttc
      541 ctccgtgtcc gccgaatttg cccaaatgcg caggcggctg acgacggcgt cgcgctgccc
      601 gccgtcggtg cgcaagtctt cccacgggaa atagccgtag cagtcctgcc atttcgcatc
      661 cgggtgcagg atgctgtcgg ctgcctcgcg caccagcccc aaatagctga cgtacaagac
      721 gggcatgccg tgttcgttgc ggcggtgggt atcgacaaag gtgtaaagct gttccacata
      781 gcccataggc tgcgaagtct gcttggcaac ccacagcttc acgcccgcct gcagggaatt
      841 gcgcagaggg gagagcgggc cgttgggcag gagtgtgcct tgggcgacgg ttaatacccg
      901 caggccgccg tcggtaacgg caatcaatac gggaaccagc tcgacgatgc tttgcggcgg
      961 ggcttcggct tcggtgtagg catccatgct cggctttctg cgggtgggaa aggctttatt
//
LOCUS       DAXHPG010000262          951 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXHPG010000262 DAXHPG010000000
VERSION     DAXHPG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.262"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            57..782
                     /locus_tag="ACFM6F_002126"
     CDS             57..782
                     /locus_tag="ACFM6F_002126"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC6165467.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAAQLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRQPSGAIVFMVGPEGGWTEQEEQQAFDAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          251 a          328 c          212 g          160 t
ORIGIN      
        1 gtccgtttcc atcacgccgc tgcgacagat tataaccgtc cgaaccgcca aaaactatgc
       61 cccgattcta cctgcccgaa aacctttccg tcggacaaac cgtcgccctg cccgacaaca
      121 tcgtccgcca cctcaacgtc ctacgcgtcc gccccaacga aaacatcacc ctcttcgacg
      181 gcaaaggcaa ggcacacgcc gcacagctga ccgttttgga aaaacgccgc gccgaagccg
      241 aaatcctgca cgaagacaca accgacaacg agtccccgct caacatcaca ctgatacaat
      301 ccatctcctc cggcgatcgc atggatttca ccctgcaaaa aagcgtcgaa ctcggcgtaa
      361 ccgccataca gcccgtcatc agcgaacgct gcatcgtccg cctcgacgga gaacgcgccg
      421 ccaaacgcct cgcacgctgg caggaaatcg tcatctccgc gtgcgaacaa agcggcagga
      481 acaccgttcc ccccgtactg cccatcatcg gctaccgtga agcactcgac aaaatgccgt
      541 ctgaaagcac caagctgatt atgagcatca accgcgcccg caaactcggc gacatccgcc
      601 aaccgtccgg cgcaatcgtc tttatggtcg ggcctgaagg cggctggaca gaacaggaag
      661 aacaacaggc atttgatgcc ggctttcagg cggttacact cggcaaacgg attttacgca
      721 cagaaaccgc cccactcgcc gccctcgccg ccatgcagac gctttggggc gatttcgcat
      781 aaacagaaat gccgtctgaa acccgttcag acggcatttt gcagccgatt aagatagtag
      841 gttcaaataa gatttcccgt gtcgtcattc ccgcgaaagc gggaatctag aaacgaaaaa
      901 ctacagggat ttatccgaaa caacaaccct ctccgccgtc attcccgcgc a
//
LOCUS       DAXHPG010000263          949 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXHPG010000263 DAXHPG010000000
VERSION     DAXHPG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.263"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(228..623)
                     /locus_tag="ACFM6F_002127"
     CDS             complement(228..623)
                     /locus_tag="ACFM6F_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6165468.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDTETDKFAKVSG"
     gene            complement(607..924)
                     /locus_tag="ACFM6F_002128"
     CDS             complement(607..924)
                     /locus_tag="ACFM6F_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC6165469.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
BASE COUNT          244 a          217 c          176 g          312 t
ORIGIN      
        1 tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta tactaattga
       61 gacctttgca aaattccttt ccctcccgac agccgaaacc caaacacagg ttttcggctg
      121 ttttcgctcc ataacacctc ctaattttac ctaaataccc ccttaatcct ccccggatac
      181 ccgataatca ggcatccggg ttacttttta ggcggcaaca ggcgcactta gcctgagacc
      241 tttgcaaatt tgtcggtttc ggtgtcgtat tggtagcctc gtgcctgtat gtcttctttg
      301 aaagtttcgt atacgtcgtg ggctaaaagg gctgttccga catagggaac cgcccttgtg
      361 ctgaatttcg cgcctaagcg ggcaagtttg ccgccccccg ccaatacgcc ggcgcgggaa
      421 atgctggcgg ttattttggc gttgattcgg gcttttgcgc ccgtagggat gtgtgttaaa
      481 tctaccgttt ttattaaatc agatgaataa gttttactat ttttaggtac aaacttatga
      541 attttcgcac cttgtccggt atcaactgaa acagtttcag atatttttac tgcatttgca
      601 ttcgcttcaa acgaatacat catcaaaatt gcaattatcg acaatttcgc aaaattcaaa
      661 tttgtatatt ttatgaccat ctttcaggga ttctttaatt accatttctg aattatcaga
      721 aaatgagatt agccaaatat catgtttaat tcttctattc cagaaaaaag agaaacaatc
      781 aataacattt tcagacttat taatcttcgc aaattcaaca aattcagatt gcgctataac
      841 cgccatcgat tgcccaaaat acttgcttga cggctgatat ttataaagtg ccaactgcgc
      901 ctgagtgata aacggcttgt tcatggttct gcctttcaat gattgtttt
//
LOCUS       DAXHPG010000264          928 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXHPG010000264 DAXHPG010000000
VERSION     DAXHPG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.264"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..>928)
                     /locus_tag="ACFM6F_002129"
     CDS             complement(<1..>928)
                     /locus_tag="ACFM6F_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6165470.1"
                     /translation="LARLGAKLSTRAVPYVGTALLAHDVYETFKEDIQAQGYQYDPET
                     DKFVKGYEYSNCLWYEDKRRINRTYGCYGVDSSIMRLMSDDSRFPEVKELMESQMYRL
                     ARPFWNWHKEELNKLSSLDWNNFVLNRCTFNWNGGDCLVNKGDDFRNGADFSLIRNSK
                     YKEEMDAKKLEEILSLKVDANPDKYIKATGYPGYSEKVEVAPGTKVNMGPVTDRNGNP
                     VQVVATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPANNPNPNEN
                     PGTSPNPEPDPDLNPDANPDTDGQPGTRPDSPA"
BASE COUNT          194 a          214 c          230 g          290 t
ORIGIN      
        1 gcgggggaat cgggtcttgt gccgggctgt ccgtccgtat cgggatttgc atcgggattc
       61 aaatcggggt cgggttcggg attggggctc gtgccggggt tctcattggg gttcgggttg
      121 tttgcggggt tttcggcggg cgatacttcg ggcagcggct gtgcgttcgg tgcttccgcg
      181 cttccggggg tcaagtcggg acgcgggatt acttgaacat ccaccgtggt gttgccttgc
      241 gaatccctgc cgaatgttgc gacaacctga acgggattcc cgttcctgtc cgtgacggga
      301 cccatattca cttttgttcc gggtgcgact tctacttttt cggaataacc gggataaccg
      361 gttgccttta tgtatttgtc gggattggca tcgactttca acgataaaat ctcttccagc
      421 tttttggcat ccatttcttc tttgtatttt gaattgcgaa taagggaaaa atcagcccca
      481 tttctgaaat catcaccttt attgaccaaa caatctccgc cattccaatt aaatgtgcaa
      541 cgatttaaaa caaaattatt ccaatccaaa gaacttaatt tattcagttc ttctttatgc
      601 caattccaaa acggacgtgc cagcctatac atttggcttt ccatcaattc tttgacttcg
      661 gggaatctgc tgtcatcgga cataaggcgc ataatcgaac tgtcaacgcc gtagcagcca
      721 taggttctat taatacgtct tttgtcttcg taccaaaggc aattactata ttcgtagcct
      781 tttacaaatt tgtcggtttc ggggtcgtat tggtagcctt gtgcctgtat gtcttctttg
      841 aaagtttcgt atacgtcatg ggctaaaagg gctgttccga cataaggaac tgcccttgtg
      901 cttaatttcg cgcctaagcg ggcaagtt
//
LOCUS       DAXHPG010000265          923 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXHPG010000265 DAXHPG010000000
VERSION     DAXHPG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.265"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            180..509
                     /locus_tag="ACFM6F_002130"
     CDS             180..509
                     /locus_tag="ACFM6F_002130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6165471.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            538..738
                     /locus_tag="ACFM6F_002131"
                     /pseudo
     CDS             538..738
                     /locus_tag="ACFM6F_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            782..923
                     /locus_tag="ACFM6F_002132"
                     /pseudo
     CDS             782..923
                     /locus_tag="ACFM6F_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          276 a          257 c          220 g          170 t
ORIGIN      
        1 aaatttaatc cactatattt ccatgaaaaa gcatggaacc tttacgaaaa aaataaaaca
       61 gtcaaacaaa cacagccgtt tcagctgcac cgatgcagct gacgtttgag cggcatttga
      121 cttcgccgga cggtatgtta cccttgccgt tttgaccaac aagcaacgaa aggacaaata
      181 tgggcggcct gattattgaa gacttgcagg aaggcttcgg aaaagaagca gttaaaggca
      241 aagagattac cgtgcattac acaggttggc tggaaaacgg aaccaaattc gactccagcc
      301 tcgaccgccg ccagccgctg accatcacgc tcggcgtcgg acaagtcatc aaaggctggg
      361 acgaaggctt cggcggaatg aaggaaggcg gcaaacgcaa gctgaccatc ccttcggaaa
      421 tgggctacgg cgcacacggt gccggcggcg tgattccccc gcacgccact ttgatatttg
      481 aagtcgagct gctgaaagtg tacgaataaa gctgcccggg caataccgtc tgacataatg
      541 gcttcaagcg gcgtaaacaa cgaaatcaaa gacaaaaaac tctccctgtg ggccaagcgt
      601 caagacggtt cggtcaaatg gttctgcgga cagccggtta cgcgcaccac cgccaccgac
      661 gtcgtcgccg ccaacggcaa gaccgacgac aaaatcaaca ccaagcacct gccgtcaacc
      721 tgccgcgatg attcatctac cggttgcata gaaacaccac gcgccgattt caaacacttt
      781 caaaaaatca gccgttaccg agtattgccc gaatcacggc acatggccga aaaacaacac
      841 ttctgccggc gtggcatctt cttcaacaat caaaggcaaa tatgttcaaa gcgttacagt
      901 cgcaaacggc gtcattaccg ccc
//
LOCUS       DAXHPG010000266          906 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXHPG010000266 DAXHPG010000000
VERSION     DAXHPG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.266"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(55..834)
                     /locus_tag="ACFM6F_002133"
     CDS             complement(55..834)
                     /locus_tag="ACFM6F_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC6165472.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          222 a          291 c          179 g          214 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ccagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgcggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcaaccaaa acattcaatg ccgaccggac cgacgggcgc gaaacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttccg caatgcgctc
      781 ctccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg cagaaccctt ccgaaatata gtggattaac aaaaatcagg
      901 acaagg
//
LOCUS       DAXHPG010000267          905 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXHPG010000267 DAXHPG010000000
VERSION     DAXHPG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.267"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..215
                     /locus_tag="ACFM6F_002134"
     CDS             <1..215
                     /locus_tag="ACFM6F_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165473.1"
                     /translation="DAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTRATLLALDWQGN
                     KPLGAAELADLKPLYKDLKNNIGNIV"
     gene            546..>905
                     /locus_tag="ACFM6F_002135"
     CDS             546..>905
                     /locus_tag="ACFM6F_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC6165474.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT          280 a          149 c          212 g          264 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggca aaaaaacacg tgcgaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg cggaactggc ggatttgaaa ccgctttaca
      181 aagacttaaa gaataatatt ggaaatattg tatgaacaaa aaattaaact atatttttat
      241 gttggactgt ttagggttgg tgatattgtt tacttgtata atagctactt ttgaaagaga
      301 ttatggattt aaaattttta ctaattctaa gaggcctgaa ttttattatt ggattggaat
      361 gttttattat ggaattattt cttgctggtt tgattatcaa ttaatttcaa caaaggcgaa
      421 ttcgtataaa agaaaagtta aacaatataa aattttttca gtaatatttt cagttttgat
      481 atttatttct actatagtaa aactttaaat tttggagcaa aaatttatga gcgattcaat
      541 tgaatatgta ttgggaacgc ggtctgcaca tgtataaggc aagtgccgtc gtgccgacgg
      601 gatatgtacg ggttgggaat accgcgccgc tggtcggcga agacacgcaa cggtatgcct
      661 ctttttgggg cgacggctac gacgtgtacc gtcagttgag atggcagcag atacccgaaa
      721 aacagagaaa ggcattcaaa aaagccgcca aaagcaaaaa gaccgtgatg tttgccggac
      781 gggaatacgg catatccaaa cagaatttga gcgatgtttg ggatgatttt gaagacgcga
      841 tggaactgaa ggcgtttccc tgcctgtctt cgctgtttct gaccaaatgg cataaaaatc
      901 tatat
//
LOCUS       DAXHPG010000268          892 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXHPG010000268 DAXHPG010000000
VERSION     DAXHPG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.268"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>892
                     /locus_tag="ACFM6F_002136"
     CDS             <1..>892
                     /locus_tag="ACFM6F_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6165475.1"
                     /translation="AKQPEYAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTGAGAAI
                     VVIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASLYSQ
                     AAVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFS
                     STGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAAS
                     KIKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATLS
                     DAEKHKVISYSKIIAGSVAAL"
BASE COUNT          261 a          218 c          238 g          175 t
ORIGIN      
        1 gccaaacagc ccgaatacgc ctacctgaaa cagcttcaga cggccaagaa cgtcgattgg
       61 aaacaggtgc agctggccta cgacaaatgg gactataaac aggaaggctt gaccggagcc
      121 ggtgcagcga ttgtggtgat tattgtaacc gctctaactt atggatacgg agcggctgca
      181 gcgggtagcg taactgccgc aggaagtagt acagccgcag ctgcaacaac gacagcggca
      241 gcaactacca ctgtttctac tgcagctgcc atgcaaaccg cagctttagc ctccttgtat
      301 agccaagcag ctgtagccat catcaataat aaaggcgatg taggcaaagc attaaaagat
      361 ctcggcacca gtgatacggt caagcagatt gttacttccg cactgacggc gggtgcatta
      421 aaccagatgg gagcagatat tgcccaattg aacagcaagg taagaaccga actgttcagc
      481 agtacgggca atcaaaccat tgccaacctt ggaggcagat tggccaccaa cctcagcaat
      541 gcaggtatct cagctggtat caataccgcc gttaacggcg gcagcttgaa agacaactta
      601 ggcaatgccg cattgggagc attggttaat agcttccaag gggaagccgc cagcaaaatc
      661 aaaacaacct tcagcgacga ttatgttgcc aaacagttcg cccacgcttt ggcgggttgt
      721 gttagcggac tggtacaggg aaaatgtaaa gatggggcaa ttggcgcagc agttggggaa
      781 atcgtagctg aatccatact tggcggcaga aaccctgcta cactcagcga tgcagaaaag
      841 cataaagtta tcagttactc gaagattatt gcgggcagcg tggcggcact ca
//
LOCUS       DAXHPG010000269          871 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXHPG010000269 DAXHPG010000000
VERSION     DAXHPG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.269"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..789
                     /locus_tag="ACFM6F_002137"
     CDS             <1..789
                     /locus_tag="ACFM6F_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6165476.1"
                     /translation="KKPSLLFSSLLFSSLLFSSAAQAASEDSGRGPYYVQADLAYAAE
                     RITHDYPKPTGANNTSTVSDYFRNIRAHSIHPRVSVGYDFGDWRIAADYASYRKWNNN
                     KYSVNTKEVRRNRGNSENLKTENQENGSFHAVSSLGLSAIYDFKLNDKFDKFKPYIGA
                     RVAYGHVKHQVHSVETETKIIFTTPTKGAKVGGTILDKSMSKPPYHESNSISSLGLGV
                     IAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYRF"
BASE COUNT          242 a          243 c          195 g          191 t
ORIGIN      
        1 aaaaaacctt ctcttctctt ctcttctctt ctcttctctt ctcttctctt ctcttccgca
       61 gcgcaggcgg caagtgaaga cagcgggcgc ggcccgtatt atgtgcaggc ggatttagct
      121 tatgccgccg aacgcattac ccacgattat ccgaaaccaa ccggtgcaaa caacacaagc
      181 acagtaagcg attatttcag aaacatccgt gcgcattcca tccacccccg agtgtcagtc
      241 ggctacgatt tcggcgactg gaggatagcg gcagattatg ccagttacag aaaatggaac
      301 aacaataaat attccgtcaa cacaaaagag gtgagaagaa acaggggcaa cagtgaaaac
      361 ctgaagacgg aaaatcagga aaacggcagc ttccacgccg tctcttctct cggcttatca
      421 gccatttacg atttcaaact caacgataaa ttcgataaat tcaaacccta tatcggtgcg
      481 cgcgtcgcct acggacacgt taaacatcag gttcattcgg tggaaacaga aaccaagatt
      541 attttcacta caccaacgaa aggcgctaaa gttggaggca cgatcctaga caaatcgatg
      601 agcaaacctc cctatcacga aagcaacagc atcagcagct tgggtcttgg cgttatcgcc
      661 ggtgtcggtt tcgacatcac gcccaagctg accttggaca ccggataccg ttaccacaac
      721 tggggacgct tggaaaacac ccgcttcaaa acccacgaag tctcattggg catgcgctac
      781 cgcttctgat tccccgatac cgatgccgtc tgaaccttca gacggcattt ttgatgcact
      841 cgccgtttac agacgcgggg cgggcgtagg g
//
LOCUS       DAXHPG010000270          802 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXHPG010000270 DAXHPG010000000
VERSION     DAXHPG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.270"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..742)
                     /locus_tag="ACFM6F_002138"
     CDS             complement(join(<1..523,523..742))
                     /locus_tag="ACFM6F_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6165477.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDSQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARNFKSMLYLACSIIHA"
BASE COUNT          192 a          172 c          160 g          278 t
ORIGIN      
        1 ttagcatgaa taatagagca agccaagtaa agcatcgatt taaaattacg agctaatttc
       61 tcatagcgag tagcaatact acgaaactgc tttaatcgtg caaatgcatt ctcgactaag
      121 tggcgtaatt tataaaggta gctatcaaaa tcagggttag gtttcttggc atttttacgc
      181 tttggtataa tagctttcat accgtgttct atcgctttat ctctgatttc ttgcgagtca
      241 taccctttgt cagcgataaa atactgggct tcttgtatga cttctatcaa gtcgtttgca
      301 acttgactgt cgtgcacgtt acccccagtg actttaaaat cgagcggatt tccatgcgag
      361 tccacacata ggtgtatttt tgtcgtgttt ccgccacggc tttgtccaat tgctctatcg
      421 aaaccacgcc gagctccact tgcatgttga tgacaccgta cataacttcc gtcgatgaat
      481 acccattctt tgtcaatttc ttttcgtaga tcaaaaaaaa attctgccac aagccttttt
      541 tagaccatcg gttaaagcgg ttataagcgg ttttccatga ccctagctca ataggtatgt
      601 ctcgccatgg tgcacctgtt cttagcttcc atagtatggc ttccatcacg gtacggtcgt
      661 tcttccattg atgacagccg tgcgctttca tggttgtttg taattgttcc catatgttgt
      721 cagttattgc ggttctcgcc attgtttgtg tttgcctata ttttgttgga atatagggtt
      781 aaaatagggc tcttttgggc tt
//
LOCUS       DAXHPG010000271          798 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXHPG010000271 DAXHPG010000000
VERSION     DAXHPG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.271"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            409..615
                     /locus_tag="ACFM6F_002139"
                     /pseudo
     CDS             409..615
                     /locus_tag="ACFM6F_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            630..798
                     /locus_tag="ACFM6F_002140"
                     /pseudo
     CDS             630..798
                     /locus_tag="ACFM6F_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tonB dependent receptor family protein"
BASE COUNT          216 a          219 c          172 g          191 t
ORIGIN      
        1 atcaaacatc aaattggact gaccaatcag ggcggattct aatgacacgc cgttcccgcc
       61 gtcaacggca tttacctcgc accgcccccg aaacacaaga aaaaactaca ccaaactaca
      121 atttttgttt atacaaatat ttgttttgac aggatttaaa cgaaagctcc gattcaaatc
      181 tgccgaaccg cccaaaaata tatagtggat taacaaaaat caggacaagg cgacgaagcc
      241 gcagacagta cagatagtac ggtaaggcga ggcaacgccg tactggttta aatttaatcc
      301 attctattga cctgaatatt aaagtttcgc aaagtaatgc aacgttgctt taattggttt
      361 gaccactatt gccgacgatt agaaaaatat tttcggagat gttcaattat ggaaacttgg
      421 gttcaaaact acacggcaat cggcggcagc ctgtatctga ctgccgccgc cgcactctta
      481 cccatcgtct ttttctttgc cgcgctgacc gtcctgaagc tgaaaggcta tcaggcgggg
      541 ctttatacgc tgctgattgc gcttgccgtt gccgtattcg gcttcgggat gccggcgggt
      601 atggcggttt cttccctgcc gccgcagccg cactgaccca acgcccctac gccacgctgg
      661 atttgaccgc gcattacaaa atcggcaaat ccacccgcat tggtttggac tttgaaaacg
      721 tgttcaacaa acgctaccgc cctatgcccg acattcacgt ttacggcacg ccgcgcagcc
      781 tgaccgcaac cgtcaaac
//
LOCUS       DAXHPG010000272          797 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXHPG010000272 DAXHPG010000000
VERSION     DAXHPG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.272"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            31..201
                     /locus_tag="ACFM6F_002141"
     CDS             31..201
                     /locus_tag="ACFM6F_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC6165478.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT          236 a          204 c          183 g          174 t
ORIGIN      
        1 atccgctata caaaaaacaa ggaaacacaa atggcgcaat atatgtgcgg cccctgcggc
       61 tggatttacg atgaagaaca cggcgacccc gaacacggca tcccccctgg gacaaagttt
      121 gaagacatcc ccgacgactg gaaatgcccc gaatgcggcg tgggcaaaga agatttctac
      181 ctgttggatt tcgtgatata gcggcagccg cacccttagg gaaaaccccg tcatccggcg
      241 ttccgcctgc ggcggcaggg aacgccgttt tatagtggat taacaaaaat caggacaagg
      301 cgacgaagcc gcagacagta caaatagtac ggaaccgatt cacttgttaa agaatcgttc
      361 tctttgagct aaggcgaggc aacgccgtac tggtttttgt taatccacta tatccgtttg
      421 cgcccgccaa gccgtgttgc acagatgcga cacggctttt ttctttctgt tgtcagaatg
      481 taaattttcg tcaaaaaaca tcaaaaatca cccccccccg aatttgcgta aaataaagtg
      541 taatcccttt gatcataagg gaatgttgac aattagatcg attctcctag gctgactttc
      601 gcaataaatg gggttgtcaa taattgtgta tggtcgtttg ccgtccggcg gccgtcattc
      661 ccgcgaacgc aggaatccag caacacaatg cagcaggact ttatcggaaa aaaccgaaac
      721 cccaccgact gtcattcccg caaaagctgg aatccaaaaa cgcaacgcaa caggaattta
      781 tcggaaaaaa cagaacc
//
LOCUS       DAXHPG010000273          792 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXHPG010000273 DAXHPG010000000
VERSION     DAXHPG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.273"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            338..583
                     /locus_tag="ACFM6F_002142"
     CDS             338..583
                     /locus_tag="ACFM6F_002142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165479.1"
                     /translation="MVLELSTYCVSPSAGSVVDFDLKKRGGVGQDVFRGTPDCGGVCL
                     YFAEGFKNRVVGYIGCTVLVCARSGQGAMGKFARDAV"
     gene            complement(603..>792)
                     /locus_tag="ACFM6F_002143"
     CDS             complement(603..>792)
                     /locus_tag="ACFM6F_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165480.1"
                     /translation="TIMRPFDGVKYKDTLTAGFSSPHHHLTADSYLQKRREPDRWYSR
                     IRRAVREAATSRAPSHDE"
BASE COUNT          143 a          154 c          282 g          213 t
ORIGIN      
        1 ggcaggaaaa agacccgcgc catatcgggg aatgaaccga cgcaaaggaa atgcggtttg
       61 aatatgcgtc cgggcggtgt tttactgtgt gtgccgtatg gcggggcagg gtgtctgatt
      121 tcgtgatgac cgtgttggtg gaatcgaagc ctgccgttcc ggtaagggtt gcccgggagg
      181 gcaaggccgg ctttgtatgc ccggttaaga gggaaggatg agactgtgca tttttgattt
      241 taattggcta tatgccgtct gaaaatcggt ttgaaggcgg tacttttatg ctgcctttca
      301 tacaggggaa tgggaacgtt gcggctgcgc ggaaaccgtg gtgcttgaac tttctactta
      361 ctgcgtttcg ccgtcggcgg gttcggtggt ggattttgac ctgaaaaaaa gggggggtgt
      421 tgggcaagat gtttttaggg gaacgccgga ttgcggtggg gtttgccttt attttgcaga
      481 aggctttaaa aaccgtgtgg tcgggtacat cggctgtacc gtgcttgttt gtgcaagatc
      541 ggggcagggt gcgatgggca agtttgcccg ggatgccgtc tgaagcgttc agacggcatt
      601 ttttattcgt cgtggcttgg tgcgcgggat gtggcggctt cccggacggc acggcggatg
      661 cggctgtacc atcggtcggg ttctcgccgc ttttggaggt aactgtcggc ggtcaggtgg
      721 tggtgcgggc ttgaaaaacc ggcggttagg gtgtctttat atttcacccc gtcaaaaggg
      781 cgcattatag tg
//
LOCUS       DAXHPG010000274          782 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXHPG010000274 DAXHPG010000000
VERSION     DAXHPG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.274"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..623
                     /locus_tag="ACFM6F_002144"
     CDS             <1..623
                     /locus_tag="ACFM6F_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6165481.1"
                     /translation="NNTSTVSDYFRNIRAHSIHPRVSVGYDFGGWRIAADYASYRKWN
                     NNKYSVNTKVLQEKNGNRKELKTENQENGTFHAASSLGLSAIYDFKLNDKFDKFKPYI
                     GARVAYGHVKHQVHSVKKETTTVFTAPTQGAQKGGVIPEATISKPAYHESHSISSLGL
                     GVIAGVGFDITPKLTLDTGYRYHNWGRLKNTRFKTHEVSLGMRYRF"
BASE COUNT          231 a          220 c          169 g          162 t
ORIGIN      
        1 caaacaacac aagcacagta agcgattatt tcagaaacat ccgtgcgcat tccatccacc
       61 cccgggtgtc ggtcggctac gatttcggcg gctggaggat agcggcagat tatgccagtt
      121 acagaaaatg gaacaacaat aaatattccg tcaacacaaa agtgttgcaa gaaaaaaatg
      181 gcaacaggaa agaactgaag acggaaaatc aggaaaacgg tacattccac gccgcttctt
      241 ctctcggctt atccgccatt tacgatttca aactcaacga taaattcgat aaattcaaac
      301 cctatatcgg cgcgcgcgtc gcctacggac acgttaaaca tcaggttcat tcagtgaaaa
      361 aagaaaccac gactgttttc actgcaccaa cgcaaggtgc tcaaaaggga ggcgttatcc
      421 cagaagctac gattagcaaa cctgcctatc acgaaagcca cagcatcagc agcttgggtc
      481 ttggtgtcat cgccggtgtc ggtttcgaca tcacgcccaa gctgaccttg gacaccggat
      541 accgctacca caactgggga cgcttgaaaa acacccgctt caaaacccac gaagtctcat
      601 tgggcatgcg ctaccgcttc tgattccccg ataccgatgc cgtctgaacc ttcagacggc
      661 atttttgatg cacctgccgt ttacagacgc ggggcgggcg tgggaatacc cgaaccgtca
      721 ttcccgacaa caccgtaatc tcgaaatttg tcattcccga caacaccgca atctcgaaat
      781 tc
//
LOCUS       DAXHPG010000275          781 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXHPG010000275 DAXHPG010000000
VERSION     DAXHPG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.275"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>781
                     /locus_tag="ACFM6F_002145"
     CDS             <1..>781
                     /locus_tag="ACFM6F_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6165482.1"
                     /translation="LLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSN
                     GDNIINAADAAFQTLRVWQDLNQDGISQTNELRTLEELGIQSLDLAYKDVNKNLGNGN
                     TLAQQGSYTKTDGTTAKMGDLLLAADNLHSRFKDKVELTAEQAKAANLAGIGRLRDLR
                     EAAALSGDLANMLKAYSAAETKEAQLALLDNLIHKWAETDSNWGKKSPMRLSTDWTQT
                     ANEGIALTPSQVAQLKKNALVSLSDKAKAAIDAARDRIAVLD"
BASE COUNT          229 a          206 c          180 g          166 t
ORIGIN      
        1 ttactcgtcc gcgatttgaa cggcaacggc atcatcgaca acggtgcgga actcttcggc
       61 gacaatacca aactggcaga cggttctttt gccaaacacg gctatgcagc tttggccgaa
      121 ttggattcaa acggcgacaa catcatcaac gcggcagacg ccgcattcca aaccctgcgt
      181 gtatggcagg atctcaacca ggacggcatt tcccaaacca acgaactccg cacccttgaa
      241 gaattgggta ttcaatcttt ggatctcgcc tataaagatg taaataaaaa tctcggtaac
      301 ggtaacactt tggctcagca aggcagctac accaaaacag acggtacaac cgcaaaaatg
      361 ggggatttac ttttagcagc cgacaacctg cacagccgct tcaaagacaa agtggaactc
      421 actgccgaac aggcaaaagc cgccaatctt gcgggcattg gtcgtctgcg cgatttgcgc
      481 gaagctgccg cattgtccgg cgatttggcc aatatgctga aagcttattc tgccgccgaa
      541 actaaagaag cacagttggc attgttagat aatttgattc acaaatgggc ggaaaccgat
      601 tcgaactggg gcaaaaaatc gccaatgcga ctttcaaccg attggacgca aacggctaat
      661 gaaggtattg cactgacacc atcccaagta gcacaactaa aaaagaacgc tttagtttcc
      721 ctttctgata aagctaaagc agctattgac gccgcccgcg accgcattgc cgtgcttgat
      781 g
//
LOCUS       DAXHPG010000276          776 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXHPG010000276 DAXHPG010000000
VERSION     DAXHPG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.276"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(168..>776)
                     /locus_tag="ACFM6F_002146"
     CDS             complement(168..>776)
                     /locus_tag="ACFM6F_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6165483.1"
                     /translation="EGLPYVAVFEDDVLLGKDAEKFLAEDTWLEERFDKDSAFIVRLE
                     TMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISREAMRFFLERFAVLPAEWIKA
                     VDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKNSMLGSDLEKDREQERRHRRS
                     LKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRVISFK"
BASE COUNT          225 a          215 c          129 g          207 t
ORIGIN      
        1 ggaaaaacag aaatcccccg ccgtcattcc cgcaaaagcg ggaatctaga aatttaacgt
       61 tgcggtgatt tatcggaaat gactgaaact caacggactg gattcccact ttcgcgggaa
      121 tgacagtgta tccatttcta attttaatcc gctatatttt acacaaacta tttgaacgat
      181 atgacccgcc tgccgtaagc tttctcaagc tccgcctgcc tttgacgctc cattcttttc
      241 ttcttttctc taccgaattt acccaaagca cgcttcaagt caaacatcac cttcaacgaa
      301 cggcggtgtc ttctttcttg ttccctatct ttttccaaat cgctacccaa catactgttt
      361 ttactgagga acttggcata atgcaattct tgggtacata aggcgggatt aacctgataa
      421 acaggcatcc cctccttatc aaagaaataa gtaaacatca tccaatctac cgctttaatc
      481 cactctgccg gcaaaacggc aaacctttcc aagaaaaacc gcatcgcctc acgcgaaatg
      541 atatagccag ccgtccccca atgttcgctc tccagcaaag gaaatgaccg gttctcataa
      601 ttcaggactt tatccggtct gacaataact ttcgcaaaca tcgtttccaa acgaacgata
      661 aaggcagaat ccttatcaaa acgctcttcc aaccaagtat cttcggcaag gaacttttct
      721 gcgtctttgc caagcaggac atcatcctca aatacggcaa catagggcag accttc
//
LOCUS       DAXHPG010000277          763 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXHPG010000277 DAXHPG010000000
VERSION     DAXHPG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.277"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>763
                     /locus_tag="ACFM6F_002147"
     CDS             <1..>763
                     /locus_tag="ACFM6F_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC6165484.1"
                     /translation="STGGLEIPAAKAIRRAGIAVIIANPRQTHQFAQSQPLTKTDAKD
                     AKMLAFFAQMMTQKEDWQTMPYQPPTEAEEVLEALVNRRNQSADMRTAEKNRLHQVHE
                     TQVGSVKQLIAHFDRLIDELDKQIDDHTHTHFDGKAQVAEQIKGIGSITTATLMAMLP
                     ELGRLSHKRIASLAGIAPHPRESEETKFKSRCFGGRSAVRKALYMATVVATRFKPLIR
                     DFYQRLPSEGKPYKVAVTAYMRKLLTISNARMRDY"
BASE COUNT          205 a          213 c          205 g          140 t
ORIGIN      
        1 aaagtactgg cggtctcgaa atccccgccg ccaaagccat ccgccgagca ggcatagccg
       61 tgattatcgc caacccgcgt cagacgcatc agtttgccca atcgcagccg ctgaccaaaa
      121 ccgacgccaa agatgccaaa atgctcgcct tcttcgcaca gatgatgacg cagaaagaag
      181 attggcaaac catgccctac caaccgccca cagaagcgga agaagtgttg gaagccttgg
      241 ttaaccgccg caaccaatcg gcggatatgc ggactgccga gaaaaaccgt ctgcatcagg
      301 ttcacgaaac gcaagtcgga agcgtcaaac aactgattgc ccattttgac cggctgattg
      361 acgaattgga caaacaaatc gacgaccaca cccacacgca ttttgacggc aaagcccaag
      421 tggcggagca aatcaaaggc atcggttcga taacgacggc tacgctgatg gcgatgctgc
      481 ctgagttggg gcggctgtcg cacaaacgga tagcgagttt ggccggcatt gccccgcacc
      541 cgagggagag cgaggaaacc aaattcaaaa gccgctgctt cggcggaagg tctgcggtac
      601 gtaaggcgct gtatatggct accgtggtag cgacacgttt taaaccgctt attcgggatt
      661 tctaccaacg cctgccgtcc gagggtaagc cgtataaggt tgccgttacg gcatatatgc
      721 gcaaactgct gacgatatcg aatgcccgga tgcgtgatta ttt
//
LOCUS       DAXHPG010000278          749 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXHPG010000278 DAXHPG010000000
VERSION     DAXHPG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.278"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(137..>749)
                     /locus_tag="ACFM6F_002148"
     CDS             complement(137..>749)
                     /locus_tag="ACFM6F_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6165485.1"
                     /translation="VSDYFRNIRAHSIHPRVSVGYDFGGWRIAADYASYRKWSNNKYS
                     VNTKEVQRNNNAGNWQELKTENQENGSFHAVSSLGLSAIYDFKLNDKFDKFKPYIGAR
                     VAYGHVKHQVHSVRKETTTTFSKAGAGATEPGKIISGPISKPAYHESHSISSLGLGVI
                     AGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
BASE COUNT          157 a          172 c          208 g          212 t
ORIGIN      
        1 cgggaatggc gggtttcaag attacggtgt tgtcgggaat gacggttcgg gtatttccac
       61 gcccgccccg cgcctgtaaa cggcgagtgc atcaaaaatg ccgtctgaag gttcagacgg
      121 catcggtatc ggggaatcag aagtggtagc gcatgcccaa tgagacttcg tgggttttga
      181 atcgggtgtt ttccaagcgt ccccagttgt ggtaacggta tccggtgtcc aaggtcagct
      241 tgggcgtgat gtcgaaaccg acaccggcga tgacaccaag acccaagctg ctgatactgt
      301 ggctttcgtg ataggcaggt ttggagatcg gacctgatat aattttgcct ggctctgtag
      361 cacctgcccc tgctttactg aaagtagtcg tggtttcttt tctcaccgaa tgaacctgat
      421 gtttaacgtg tccgtaggcg acgcgcgcac cgatataggg tttgaattta tcgaatttat
      481 cgttgagttt gaaatcgtaa atggcggata agccgagaga agagacggcg tggaagctgc
      541 cgttttcctg attttccgtc ttcagttctt gccagttgcc agcgttattg tttctttgca
      601 cctcttttgt gtttacggaa tatttattgt tgctccattt tctgtaactg gcataatctg
      661 ccgctatcct ccagccgccg aaatcgtagc cgaccgacac ccgggggtgg atggaatgcg
      721 cacggatgtt tctgaaataa tcgcttact
//
LOCUS       DAXHPG010000279          749 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXHPG010000279 DAXHPG010000000
VERSION     DAXHPG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.279"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..438
                     /locus_tag="ACFM6F_002149"
     CDS             <1..438
                     /locus_tag="ACFM6F_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6165486.1"
                     /translation="DHTHTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIA
                     SLAGIAPHPRESGETKFKSRCFGGKSAVRKALYMATVAATRFEPLIRDFYQRLPSEGK
                     PYKVAVTACMRKLLTISNARMRDYFAENDTAENDTAENGIRTA"
BASE COUNT          177 a          182 c          215 g          175 t
ORIGIN      
        1 gaccacaccc acacgcattt tgacggcaaa gcccaagtgg cggagcaaat caaaggcatc
       61 ggttcgataa cgacggctac gctgatggcg atgctgcccg aattggggcg gctgtcgcac
      121 aaacggatag cgagtttggc cggcattgcc ccgcacccga gggagagcgg ggaaaccaaa
      181 ttcaaaagcc gctgctttgg cggaaagtct gcggtacgta aggcgctgta tatggctacc
      241 gtggcagcga cacgttttga accgcttatt cgggatttct accaacgcct gccgtccgag
      301 ggtaagccgt ataaggttgc cgttacggca tgtatgcgca aactgctgac gatatcgaat
      361 gcccggatgc gtgattattt tgccgaaaac gataccgccg aaaacgatac cgccgaaaac
      421 ggtatccgga cggcttgatt tgagttttgg tatttttgcc cgacggggtg aaaaatacag
      481 ttgctacggc tcgatgaatc gtcagaaata cccgaaccgt cattcccgcg caggcgggaa
      541 tctagaccgg tcggtgcgga aacttatcgg gaaaaaaggt ttctccgtcc ctgaatcctg
      601 gattcccact ttcgtgggaa tgacagaatg taggttcgtg ggaatgacgt ggtgcaggtt
      661 tccgtgcgga tgaattcatc attcccgcgc aggcgggaat ctagtctgtt cggtttcagt
      721 tattttcgat aaatgcttgt tgcttttca
//
LOCUS       DAXHPG010000280          723 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXHPG010000280 DAXHPG010000000
VERSION     DAXHPG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.280"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..492
                     /locus_tag="ACFM6F_002150"
     CDS             <1..492
                     /locus_tag="ACFM6F_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HFC6165487.1"
                     /translation="IYNTLLMVEGKQLDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFL
                     AFAEQDAEAMKAALEPLFDKKTARMAAKETLSYFDFYLQPQIVTYAKIASMHGFDLGI
                     DHEIAPRDLTVYDPLPADEYQDIFDFMKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNA
                     KRE"
BASE COUNT          203 a          169 c          167 g          184 t
ORIGIN      
        1 atttacaata ctctgctgat ggtggagggt aagcagcttg atcggttgaa acaacgtagc
       61 gagaaagtct tggcgcatcc cacccctagc aaatggctgc aaaagcggtt gtacgattac
      121 cgcttcttcc tcgctttcgc cgaacaggat gccgaggcga tgaaggccgc cttagagccg
      181 ctttttgata aaaaaaccgc gcgtatggct gccaaagaaa cattgtccta tttcgatttc
      241 tacctgcagc cgcaaatcgt tacctacgcc aaaatcgcat ccatgcacgg tttcgatttg
      301 ggcatagacc acgaaatcgc gccgagggat ttgactgttt acgatccgct gccggcagac
      361 gaatatcaag acatcttcga ttttatgaaa cagtatgact tgtcttaccc gtatgaatat
      421 ctgcaggatt ggatagatta ctatacgttc aaaaccgata agctggtatt tggtaacgcg
      481 aagcgagagt gagccgtaaa actctgagct cctgttttat agattacaac tttaggccgt
      541 cttaaagctg aaagattttc gaaagctata aattgaagcc cttccacagt acatagatct
      601 gtgttgtggc ggggctttac cacgctgatt gccggagaag aactcaacct gctggcaaaa
      661 caaggcatga gatctttgca ataacatgag ttgagacctt tgcaaaaaag cccttccccg
      721 aca
//
LOCUS       DAXHPG010000281          687 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXHPG010000281 DAXHPG010000000
VERSION     DAXHPG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.281"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>687
                     /locus_tag="ACFM6F_002151"
     CDS             <1..>687
                     /locus_tag="ACFM6F_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6165488.1"
                     /translation="RGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRF
                     DELSIPDYSTLCRYRNWLAQDDTLSELLELINCQLTEKGLKIEKASAAVVDATIIQTA
                     DSTQRQAIEVDEEGQINGQTTPSKDKDARWIKKNGLYKLGYKQHTRTDGEGYIEKLHI
                     TPANAHECKHLSPLLEGLPKGTTVYADKGYDSKENRQHLKEHRLQNGIMRKACRNRPL
                     TETQTKRNRY"
BASE COUNT          206 a          213 c          150 g          118 t
ORIGIN      
        1 ccgcggccgt cccgcctatc ccctgttgtc catgttcaaa gccgtcctgc tcggacaatg
       61 gcacagcctc tccgatcccg aactcgaaca cagcctcatc acccgcatcg atttcaacct
      121 gttttgccgt tttgacgaac tgagcatccc cgattacagc accttatgcc gctaccgcaa
      181 ctggctggct caagacgaca ccctgtccga actgttggaa ctgattaact gccaactgac
      241 cgaaaaaggc ctaaaaatag agaaagcatc cgccgccgtc gttgacgcca ccattattca
      301 gaccgccgac agcacacagc gtcaggccat agaagtcgat gaagaaggac aaatcaacgg
      361 ccaaaccaca ccgagtaagg acaaagatgc ccgttggata aagaaaaacg gcctctacaa
      421 actcggttac aaacaacata cacgtaccga tggggaaggc tatatcgaga aactgcacat
      481 cacccccgcc aatgcccatg agtgcaaaca cctgtcaccg ttgttggaag gactgcccaa
      541 aggtacgacc gtctatgccg acaaaggcta cgacagcaag gaaaaccggc aacatctgaa
      601 agaacatcgg ttgcagaacg gcattatgcg caaagcctgc cgcaaccgtc cgctgacgga
      661 aacgcaaacc aaacgtaacc gatattt
//
LOCUS       DAXHPG010000282          681 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXHPG010000282 DAXHPG010000000
VERSION     DAXHPG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.282"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(80..565)
                     /locus_tag="ACFM6F_002152"
     CDS             complement(80..565)
                     /locus_tag="ACFM6F_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC6165489.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          199 a          215 c          123 g          144 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatatttt caaaccggaa aaatgccgtc tgaaccttca
       61 gacggcattc cattcacatt tattcgtctt ttttgtgttc gatggcgttt cgggaacgat
      121 tgccgccgta aggctcttca accgtatatt ccccctcgat aatatcgtca tcgcgggaaa
      181 agccctcttt tctgcccgat tggttcatgt tgaaaaaatt ttccgcacct cctgcctgca
      241 acactgcacc tcccttaaac ggcagcagca gcaataccgc caacaccgag gatacgaatc
      301 ccggactcat cagacacacc gccgccaccg tataacggat aggccacaac atctgataaa
      361 cggataccct cccgccgctt ctcattgccg cacccgccaa taaaagaccg gacagccccg
      421 tatgcctgag catcagcaca ccggcggcaa aggttgccgc catcagaaac agcgtccaac
      481 cgccgcccag ccaatcggca acccacacaa tcgacataat ctccaaaaac agcagcacca
      541 aaaaaccgat accgaaaaat ctcattgacc gtcatcctta tatttaagta aacagcaaac
      601 cgcccgaaca agactccaag cgacctgcct gtaaatgatt acaaaaccat gtgcttcaag
      661 ccgaaacaat gtgaaatcac g
//
LOCUS       DAXHPG010000283          675 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXHPG010000283 DAXHPG010000000
VERSION     DAXHPG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.283"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(89..640)
                     /locus_tag="ACFM6F_002153"
     CDS             complement(89..640)
                     /locus_tag="ACFM6F_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165490.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNILQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKAGGAVKAGG"
BASE COUNT          187 a          271 c          127 g           90 t
ORIGIN      
        1 aagaaaccgc cattagcact acaccccccc aagctgcggc tgccaaacac atcggctgct
       61 tctcgacgca cccgctgaaa aatgcgccct atccgcccgc cttgaccgca ccccctgcct
      121 ttgccgccct gcccgatgcc gtctgaagcc tgaaccgccc caccagcccg atgacgcacg
      181 acaccgccgg aaagggcata cacaaagccg tcagcacata acgcgcattc cccgtattga
      241 ccacgtccgc gcccttcagc agcagcggca gcgcgttcgc ccactcccac accgacggcg
      301 cactgaacgc caacaccgcc accagcccca acatccggaa caccccgatt tccaacacct
      361 gccgccgccg caccgaacgg ttgagcagaa acacaaccgc caacccgaaa cagaccaccg
      421 ccgccatacc gccgttggcg acaatctgca aactgttgaa tgcaatatcg ggcgtaaccg
      481 ccaacacgcc ttccgaatcc ggcacggcac gctgcaaatc caaaccctgc aagatattca
      541 acacaaaacc gtagatcaaa tcaaaaaaca ccacgcctat cggcaaagca ctcaaaaccc
      601 gccacatctt cctgtccgac caaccgttca aaactttcat tttacccgac cacgcaagcc
      661 gccgaacaaa aaata
//
LOCUS       DAXHPG010000284          660 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXHPG010000284 DAXHPG010000000
VERSION     DAXHPG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.284"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..101)
                     /locus_tag="ACFM6F_002154"
     CDS             complement(<1..101)
                     /locus_tag="ACFM6F_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6165491.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVE"
     gene            complement(224..613)
                     /locus_tag="ACFM6F_002155"
     CDS             complement(224..613)
                     /locus_tag="ACFM6F_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165492.1"
                     /translation="MWKITKEQGQDLSFATYCLLSAAINLQEFKLWLEKVVLDMPIDN
                     IHFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALTGIAFLRGIDVYDPPVSKEK
                     ALKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT          188 a          119 c          147 g          206 t
ORIGIN      
        1 tgttcgacgg caaagcgttt gccgccgccg tgggcgggta tgccggtcag tgtgccgcag
       61 gcggttagga cgagggggat gaggaggagc agggttttca tagcggggtt tgtttgatgt
      121 tgaacggatt ttgagtgtaa agggatttta agggtttgta aacaaaaggg gcgaaaatgc
      181 cgtctgaacg gcggaacggt ctttcagacg gcattttgac tgtttaaagc gggggcagtt
      241 ctacaaacgg aaagaaatgc tgaaacctct gataaatttc aggatgtttc tttaaggctt
      301 ttaatgcttt ttcttttgaa acgggagggt catagacatc tatacccctt aagaaggcaa
      361 tgcctgttaa tgcgtcatct tggtctttag ataagtctga attcggaaca aaatccaaaa
      421 tattataaat atctcctact ccttctttaa gatcaattaa atcaaaaata taaaaatgta
      481 tattatcaat aggcatatct aagacaacct tctccaacca taatttaaat tcttgcaaat
      541 taattgctgc cgataataag caatatgttg caaaacttaa atcttgacct tgctctttcg
      601 taattttcca cataattatc ttcctttgcc gtcaaacgct cttttggtta cctgctttgt
//
LOCUS       DAXHPG010000285          658 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXHPG010000285 DAXHPG010000000
VERSION     DAXHPG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.285"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..>658)
                     /locus_tag="ACFM6F_002156"
     rRNA            complement(<1..>658)
                     /locus_tag="ACFM6F_002156"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          132 a          213 c          142 g          171 t
ORIGIN      
        1 ttttaatctt gcgaccgtac tccccaggcg gtcaatttca cgcgttagct acgctaccaa
       61 gcaatcaagt tgcccaacag ctaattgaca tcgtttaggg cgtggactac cagggtatct
      121 aatcctgttt gctacccacg ctttcgggca tgaacgtcag tgttatccca ggaggctgcc
      181 ttcgccatcg gtattcctcc acatctctac gcatttcact gctacacgtg gaattctacc
      241 tccctctgac acactcgagt cacccagttc agaacgcagt tcccgggttg agcccgggga
      301 tttcacatcc tgcttaagta accgtctgcg cccgctttac gcccagtaat tccgattaac
      361 gctcgcaccc tacgtattac cgcggctgct ggcacgtagt tagccggtgc ttattcttca
      421 ggtaccgtca tcagccgctg atattagcaa cagccttttc ttccctgaca aaagtccttt
      481 acaacccgaa ggccttcttc agacacgcgg catggctgga tcaggcttgc gcccattgtc
      541 caaaattccc cactgctgcc tcccgtagga gtctgggccg tgtctcagtc ccagtgtggc
      601 ggatcatcct ctcagacccg ctactgatcg tcgccttggt aggcctttac cccaccaa
//
LOCUS       DAXHPG010000286          622 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXHPG010000286 DAXHPG010000000
VERSION     DAXHPG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.286"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(29..>622)
                     /locus_tag="ACFM6F_002157"
     CDS             complement(29..>622)
                     /locus_tag="ACFM6F_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6165493.1"
                     /translation="EGLPYIAVFEDDVLLGEGAEKFLAEDAWLKERFDPDSAFIVRLE
                     TMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISKKAIRFFLQHFVMLQPEQIKP
                     IDLMMFSDFFDKEGMPVYQVSPALCAQELHYAKFHDQNSALGSLIEHDRLLNRKQQRR
                     DSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
BASE COUNT          139 a          161 c          164 g          158 t
ORIGIN      
        1 gcaaatacga tgtccatctc tcctcctttt attggaaagg cacaatgaac tgttcgcgcc
       61 tttgccggcg tttttccctt tccctgctga ttttggtcaa ggcgcggatc aggcggcgtt
      121 tgaatgtgtt ggcgggggaa tcgcgccttt gctgtttgcg gttcaggagg cggtcgtgtt
      181 cgatcaggct gcccaatgcg ctgttttggt cgtgaaactt ggcataatgc agctcttggg
      241 cgcataaggc cggactgacc tgataaacag gcatcccctc cttatcgaaa aaatcactga
      301 acatcattaa gtcgataggt tttatctgtt ccggctgcaa cattacgaaa tgttgcagga
      361 aaaaccgtat cgcttttttg gaaatgatat agcccgccgt cccccagtgt tcgctttcca
      421 acagcggaaa ggcacgcccg cagtagtccg ccacaccgga gggcgaggtc aggacgtgca
      481 taaacatcgt ttccaagcgg acgataaagg cggaatcggg gtcaaagcgt tcttttagcc
      541 aagcgtcttc ggcaaggaat ttctccgcgc cttcgccgag taaaacgtcg tcctcaaata
      601 cggcgatata cggcagacct tc
//
LOCUS       DAXHPG010000287          618 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXHPG010000287 DAXHPG010000000
VERSION     DAXHPG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.287"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..376
                     /locus_tag="ACFM6F_002158"
     CDS             <1..376
                     /locus_tag="ACFM6F_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6165494.1"
                     /translation="QSVEVKNGVVTATMASSNVNNEIKGKKLSLWAKRQDGSVKWFCG
                     LPVARDGTDSATDVKADATANINTKHLPSTCRDDSSAVCTRTPRADFKHFQKISRYRV
                     LPESRHMAEKLRHSHESGNLVR"
BASE COUNT          184 a          174 c          133 g          127 t
ORIGIN      
        1 tcaaagcgtt gaagtcaaaa acggcgtcgt taccgccaca atggcttcaa gcaacgtaaa
       61 caacgaaatc aaaggcaaaa aactctccct gtgggccaag cgtcaagacg gttcggtaaa
      121 atggttctgc ggactgccgg ttgcgcgcga cggcaccgac agcgccaccg acgtcaaagc
      181 cgacgccacc gccaacatca acaccaagca cctgccgtca acctgccgcg atgattcatc
      241 tgccgtttgc acaagaacac cacgcgccga tttcaaacat tttcaaaaaa tcagccgtta
      301 ccgagtattg cccgaatcac ggcacatggc cgaaaaactt cgtcattccc acgaaagtgg
      361 gaatctagtt cgttgagttt cagttattta gaataaattt tgaaacttta atcccgtcat
      421 tcccgcgaaa gtggggatcc agaacgcaaa atctcaagaa accgttttcc cgataaattt
      481 ccgtgccgac gaggctggat tcccgcctgc gcgggaatga cgaagctatc catacggaaa
      541 tctacatccc gtcattccca cgaaagtggg aatccagaac gcaaaatcta aagaaaccgt
      601 tttcccgata agtttctg
//
LOCUS       DAXHPG010000288          608 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXHPG010000288 DAXHPG010000000
VERSION     DAXHPG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.288"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>608
                     /locus_tag="ACFM6F_002159"
     CDS             <1..>608
                     /locus_tag="ACFM6F_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6165495.1"
                     /translation="FKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYSTL
                     CRYRNWLAQDNTLSELLELINRQLTEKGLKIEKASAAVVDATIIQTAGSKQRQAIEVD
                     EEGQISGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDVEGYIEKLHITPANAHECKHL
                     PPLLEGIAEGTTVYADKGYDSAENRQHLEERQLLDGIMRKACR"
BASE COUNT          180 a          175 c          140 g          113 t
ORIGIN      
        1 ttcaaagccg tcctgctcgg acaatggcac agcctctccg atcccgaact cgaacacagc
       61 ctcattaccc gcatcgattt caacctgttt tgtcgttttg acgaactgag catccccgat
      121 tacagcacct tatgccgcta ccgtaactgg ctggcgcaag acaacaccct gtccgaattg
      181 ctggaactga tcaaccgcca actgaccgaa aaaggtttaa aaatagagaa agcatccgct
      241 gccgttgttg acgccaccat tattcagacc gccggcagca aacagcgtca ggccatagaa
      301 gttgacgaag aaggacaaat cagcggccaa accacaccga gtaaagacag cgatgcccgt
      361 tggataaaga aaaacggcct ctacaaactc ggttacaaac aacatacccg taccgatgtg
      421 gaaggctata tcgagaaact gcacattacc cccgccaatg cccatgagtg caaacacctg
      481 ccgcctttgc tggaagggat agctgaaggt acgaccgtct atgccgacaa aggctatgac
      541 agtgcggaaa accggcaaca tctggaagag cgtcagttgt tagacggcat tatgcgcaaa
      601 gcctgccg
//
LOCUS       DAXHPG010000289          593 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXHPG010000289 DAXHPG010000000
VERSION     DAXHPG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.289"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>593
                     /locus_tag="ACFM6F_002160"
     CDS             <1..>593
                     /locus_tag="ACFM6F_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6165496.1"
                     /translation="AAIGYATGGSGGAVVGANVDWNNRQLHPSEYKLAEQYADIVASR
                     LNISKEEALGRIIRHMQRTVDYATAKADNFRTDDAIISILGANNLPKQDQHYTNPNYN
                     KQYIQNYVRDFNLANKYNYFGKTPTQSRDYKVGVVTDIGKAALNSGIGIFESAANLAT
                     GEIDPNSWAYVHMGRIGYTHPEYGNAWIRISSATLVY"
BASE COUNT          194 a          101 c          135 g          163 t
ORIGIN      
        1 gcggccatcg gctatgcaac tggtggtagt ggtggtgctg tggtgggtgc gaatgtagat
       61 tggaacaata ggcagctgca tccgagtgaa tataaattag cagaacaata tgcagatata
      121 gtggcttctc gtctaaacat ttcaaaagaa gaagcacttg ggcgaattat tcggcatatg
      181 caacgaaccg tagattacgc aacggctaaa gcggataatt tccgtactga tgacgccatt
      241 atttcaattt taggagcaaa caatttaccc aagcaagatc aacattacac taatccgaac
      301 tacaacaaac aatacattca aaattatgtt cgtgatttca atttagccaa taaatacaat
      361 tattttggga aaactcctac tcaatcaagg gattataaag ttggagttgt tacagatatt
      421 ggtaaggctg cattaaatag cggtataggt atatttgaat cggctgcgaa tttagcgaca
      481 ggggagatag atccgaactc atgggcttat gtacacatgg ggcgtatagg ttatacacat
      541 cctgaatatg gaaatgcttg gattcggatt tcaagtgcaa cactagtgta tta
//
LOCUS       DAXHPG010000290          585 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXHPG010000290 DAXHPG010000000
VERSION     DAXHPG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.290"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
BASE COUNT          137 a          174 c          136 g          138 t
ORIGIN      
        1 atcggtttgt cggcgtttca tttgccccca tcccgaaaac gcaaacaata ccgtctgaaa
       61 gctctgtagg gagggcttca gcccaccgat tccatcaatc ccacatttcc gccgttttca
      121 ccgaatccgc cccttttcac aatccagtcc tacgcaactg aaccgtcatt cccgcgcagg
      181 cgggaatccg gacttgtcgg cacggaaact tatcgggtac aacggtttct tgagattccg
      241 catcctagat tcccgcctgc gcgggaatga cggcgcataa gtttctgtgt tgatttgagt
      301 ctatgggaac ggtgtaggat tggtggaatc gttggaatcg gtgggctgaa gcccacccta
      361 taactcacct tttacgaatc ctcctacacc ctatacaaca ccttgaaaat ccaccttgtc
      421 gggaatgccc gaaccgtcag aaatacccga atcgtcattc ccgcgcaggc gggaatccag
      481 acctgtcggt gcagaaactt atcgggcaaa acggtttctt gagattccgc atcctagatt
      541 cccgcctgcg cgggaatgac gaatccatcc gtacggaaac ctgca
//
LOCUS       DAXHPG010000291          578 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXHPG010000291 DAXHPG010000000
VERSION     DAXHPG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.291"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(131..424)
                     /locus_tag="ACFM6F_002161"
     CDS             complement(131..424)
                     /locus_tag="ACFM6F_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HFC6165497.1"
                     /translation="MNIDDFTPSKIANFFADPDTYSNVLEEVSRFIYSLVPDDANPWK
                     GGEDYIGRGISEWGELLEKWYKQDFLPYLEKNGTNFRNLKIGCLNSLNGQESG"
BASE COUNT          165 a          127 c          100 g          186 t
ORIGIN      
        1 ccttggctgc ggtggtcgaa caatgcacct gcaaagcctt tggcggcgac agtttctatg
       61 ccgtcaccgt ccaaatctag ggcaaggggg tcgtaaacgt tatatttgcc tgaacgtttg
      121 agagctaatt tcaaccactc tcttgcccat tcagggaatt caggcagcca atcttcaaat
      181 ttcggaaatt ggtcccattc ttttcaagat aagggagaaa atcttgttta taccattttt
      241 ccagtaactc tccccattca cttatccctc gtccaatata atcttcgccc cctttccaag
      301 ggtttgcatc atcaggaact aaggaatata taaacctaga tacttcttct aatacattgc
      361 tgtatgtatc aggatccgca aaaaagtttg ctatcttact tggtgtaaaa tcatctatat
      421 tcattataga ttcgccaagc ctctcgtttc cataatcctt atttaattca attcatgctg
      481 taaaacagta gaggatttta gcatttttct cgtatatctc gagatacatt tcttaaattt
      541 aaaaaaatgt tatcgaggga tgcaagtaaa gaagcagt
//
LOCUS       DAXHPG010000292          576 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXHPG010000292 DAXHPG010000000
VERSION     DAXHPG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.292"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            167..>576
                     /locus_tag="ACFM6F_002162"
     CDS             167..>576
                     /locus_tag="ACFM6F_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6165498.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDLRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRS"
BASE COUNT          195 a          113 c          116 g          152 t
ORIGIN      
        1 gcaaaatcac gacacactcg gacacccgcc ccgcttatcg caactttgcg aacgccccgg
       61 aaacagcaaa tacagcaaat aattgatttt attagagttt atttgcaaaa ccatttgccg
      121 ttacacaaga atggcacatt aaaatgactg atgaggattt ataacgatga agacagacat
      181 tcaaaccgaa ttaacccaag ccctactacc acacgaaaaa gtatgggcga acgaagaaaa
      241 aaccatttta gccaaaaaca ttctgttgga tttggtggaa aaaaccgacc cgaccattat
      301 cggtttgtta ttgaataatg atgagttaaa acgccatttc tttgtggaag tgaatggtgt
      361 tttggtgttt aaattgcagg atttacgttt tttcttggac aaacacagcg tcaataattc
      421 ctacacaaaa tacgccaacc gcattggttt gacggacggc aaccgctttt tgaaagacaa
      481 ttcggatatt gtgttggatt ttccgtttaa agattgcgta ttgaatggcg gacaaagcac
      541 cgaagaaggc gaagagattt attttaaacg cagtaa
//
LOCUS       DAXHPG010000293          568 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXHPG010000293 DAXHPG010000000
VERSION     DAXHPG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.293"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>568
                     /locus_tag="ACFM6F_002163"
     CDS             <1..>568
                     /locus_tag="ACFM6F_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6165499.1"
                     /translation="LDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEGRRLM
                     ADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNVGFGHNKNVSLYGNDG
                     NDTLIGGAGNDYLEGGSGSDTYVFGEGFSQDTVYNYDYATGRKDIIRFTDGITADMLT
                     FTREGNHLLIKAKDGSGQVTVQSYFQNDG"
BASE COUNT          163 a          110 c          144 g          151 t
ORIGIN      
        1 ctttggattt tagtggtctt gttcaagcat ttaaccatgt caaagaaact aatccgcaaa
       61 aagcttttgt ggatttggcc gagatgcttg catatggcga acttcgttct tggtatgaag
      121 gccgaagact aatggccgat tatgtggagg aggcaaaaaa agcaggtaaa tttgaagatt
      181 accagaaagt gttgggtcag gagaccgttg cattattagc taaaacatcg ggtacgcaag
      241 cagatgatat cctgcaaaat gtaggctttg gtcataataa aaatgtttct ttatatggta
      301 atgacggcaa cgacactcta atcggcggtg ccggtaatga ttacttggag ggcggcagcg
      361 gttcggatac ttatgtcttc ggcgaaggct tcagtcagga tacggtctat aattacgact
      421 acgctaccgg acgcaaagac atcatccgct ttaccgacgg tattacagcc gatatgctga
      481 cttttacccg agagggcaac catcttctta tcaaggcaaa agacggcagt ggacaagtga
      541 ctgttcagtc ctatttccag aacgatgg
//
LOCUS       DAXHPG010000294          561 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXHPG010000294 DAXHPG010000000
VERSION     DAXHPG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.294"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(91..375)
                     /locus_tag="ACFM6F_002164"
     CDS             complement(91..375)
                     /locus_tag="ACFM6F_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6165500.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          171 a          147 c           91 g          152 t
ORIGIN      
        1 cgcgggaatg acggtttaga agttgcccga aacctcaaaa aaaaaccgaa accgaacaag
       61 ccggattccc gcctgcgcgg gaatgacggg ctaaataata tcaaaccata aatcctgcca
      121 agaaacatta ttttcttcaa tcagttgcaa tttccaagcc ctgttccatt tcttcaactg
      181 tttttcccga gtaattgcac tctccatcgt aggatgcagt tcataccaaa ccagcatagt
      241 aacgttgtac cgtgatgtaa atccctcaat caaatgctcc ctatgttggt aaatacgttg
      301 caccaaatca gatgtaacgc caatgtataa cgtgccatta cgttggcttg ctaaaatata
      361 aaccgcaggc tgcatataat acccttttga attatttcaa tttatattcc cgcgaacacc
      421 atcccgtgat tactttaacc cttcgttatt cccatagctt tccatcattc ccgcaactct
      481 tcgtcattcc cgcgaaagtg ggaatctaga acgcaaaatc taaagaaacc gttttacccg
      541 ataagtttcc gcaccgacaa a
//
LOCUS       DAXHPG010000295          552 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXHPG010000295 DAXHPG010000000
VERSION     DAXHPG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.295"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
BASE COUNT          109 a          165 c          156 g          122 t
ORIGIN      
        1 atggcggcat aaagcgcggt atggcggaaa acccgccttt ttaaagtttt attcatcgta
       61 tttccttttc ggtcaaaccc cccgcccttc ggggcggcag gatcaggctt tgtccgggag
      121 gggtgcaagc ccttccgaat cagggcggcg ccttatgtgc cgccctgcgt gttggaacat
      181 attttatccc ttaaatcttg cttcgcggga taccgtcggc ggcaggcgcg ccgcaaacgg
      241 cgggattcgg caaaccgccc gattccgcgc ccgccgaatt gctggttgga aaaccccttt
      301 ggcccggctg ccaaaggggg atgttcggaa aagtaacacg cccctttggc cggatgcaat
      361 gtatagggtt accgcattat tctacccttg cgcgctgatc atggtaaagc ggttttcggc
      421 aatccgcaac cgcctccttg gcccgccatt gccgcgaaag cgggaatccg gaacgtaaaa
      481 tctcaaaaaa accgttttat ccggcaagtt tgcccgccgg caggcccgcc attgccgcct
      541 gcgcggcaat gg
//
LOCUS       DAXHPG010000296          542 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXHPG010000296 DAXHPG010000000
VERSION     DAXHPG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.296"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>542
                     /locus_tag="ACFM6F_002165"
     CDS             <1..>542
                     /locus_tag="ACFM6F_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFC6165501.1"
                     /translation="IVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSA
                     LKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVE
                     RGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQ
                     VLAKPGTITPHTKFKAEVYVL"
BASE COUNT          144 a          135 c          144 g          119 t
ORIGIN      
        1 atcgtgttca tgaacaaatg cgacatggtc gacgatgccg agctgttgga actggttgaa
       61 atggaaatcc gcgacctgct gtccagctac gacttccccg gcgacgactg cccgattgta
      121 caaggttctg cactgaaagc cttggaaggc gatgccgctt atgaagagaa aatcttcgaa
      181 ctggctgccg cattggacag ctacatcccg actcccgagc gtgccgtgga caaaccgttc
      241 ctgctgccta tcgaagacgt attctctatt tccggtcgtg gtacagtagt aaccggtcgt
      301 gtagagcgcg gtatcatcca cgttggtgac gagatcgaaa tcgtaggtct gaaagaaact
      361 caaaaaacca cttgtaccgg tgttgaaatg ttccgcaaac tgctggacga aggtcaagca
      421 ggcgacaacg taggcgtatt gctgcgcggt accaaacgtg aagacgtaga gcgtggtcaa
      481 gtattggcta aaccgggtac aatcactcct cacaccaagt tcaaagcaga agtatacgta
      541 ct
//
LOCUS       DAXHPG010000297          533 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXHPG010000297 DAXHPG010000000
VERSION     DAXHPG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.297"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..264)
                     /locus_tag="ACFM6F_002166"
     CDS             complement(<1..264)
                     /locus_tag="ACFM6F_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HFC6165502.1"
                     /translation="MFRILIISFLFLLSAKSFSADLEIKNGKLMYALSEKYNDNGFKA
                     YKVLGEGGGIHTEYNYKFDKSLNLNVLESSTGARSLEKVPVKVT"
     gene            complement(218..508)
                     /locus_tag="ACFM6F_002167"
     CDS             complement(218..508)
                     /locus_tag="ACFM6F_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165503.1"
                     /translation="MNKNERDFFYISNSDLDKLSESYPDRPLSYVFYCYLKETGLLKN
                     FSMDKCHNFFNRINFNESCFEIKFKDDSFFIIGNGKIDVSDSNNFFSVSFEC"
BASE COUNT          199 a           89 c           59 g          186 t
ORIGIN      
        1 agttacttta acggggactt tttcaagaga gcgtgcgcct gttgaacttt ctaatacatt
       61 caaattcaaa cttttatcaa atttgtaatt atattctgta tgaattcctc ctccctcacc
      121 taaaactttg tatgccttaa atccattatc gttatatttt tccgaaagtg catacatcaa
      181 tttcccattt ttaatttcta aatctgctga aaaagattta gcactcaaaa gaaacagaaa
      241 agaaattatt agaatccgaa acatcaattt ttccattgcc aataatgaaa aatgaatcat
      301 ccttgaattt aatttcaaag caagattcat taaaattaat tctattaaaa aaattatgac
      361 atttatccat tgagaaattt ttcaatagac cagtttcttt caaataacaa taaaacacat
      421 aagaaagagg cctatcagga taagattctg acaatttatc taaatcagaa tttgatatat
      481 agaaaaagtc acgttcattt ttattcatgg tttcaagcct caaatgtcta att
//
LOCUS       DAXHPG010000298          516 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXHPG010000298 DAXHPG010000000
VERSION     DAXHPG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.298"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
BASE COUNT          113 a          119 c          145 g          139 t
ORIGIN      
        1 ggcgaaccaa cgctgtactg gtttagattt aattcactat acctgcgtaa tggtatcggg
       61 tactcatcat caacctgcct tgcattcggt tgtgttgtcc ggcaactgtc cggttgtgtc
      121 gatgggttgc cctgccgctc cctgtgctgc gcaacggttg tgtgccttgg gtgggcgcga
      181 gtggcggccg ggcggtttgt tgctatgata cgggagttcc gatatacaag ggtgggcttc
      241 agcccaccaa tcaacgaccg aaaaagaaaa aacggtgggc tgaagcccac tctacgcaat
      301 tacgcaactg ttatcgtggg gatgacggga ttttaggttt ttgtttttct gtttttgcgg
      361 gaaagatggg ttttaggttg cggtcattta tcggaaaagc agaaatcgct ccgccgtcat
      421 tcccgcgaaa gcgggaatct gaacacgtcc gtagggaaac ctatatcccg tcattcccac
      481 gaaagtggga atctagaacc tctgaatttt cagaca
//
LOCUS       DAXHPG010000299          448 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXHPG010000299 DAXHPG010000000
VERSION     DAXHPG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.299"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            79..448
                     /locus_tag="ACFM6F_002168"
                     /pseudo
     CDS             79..448
                     /locus_tag="ACFM6F_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218444.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          130 a          130 c          106 g           82 t
ORIGIN      
        1 acgtaaaatc tcaagaaacc gttttcccga taagtttctg caccgacaga cctggattcc
       61 cgcctgcgcg ggaatgacga atccattctt ttggccgaag gtcaaaaatc agccgttacc
      121 gagtattacc tgaatcacgg caaatggccc ggcggcaaca gtgatgccgg cgtggcatct
      181 tcttcaacaa tcaaaggcaa atatgttgag aaagttgaag tcgcaaaagg cgtcattacc
      241 gccacaatgc tttcaaccgg cgtaaacaaa gaaatccaag gcaaaaaact ctccctgtgg
      301 gccaagcgtc aagacggttc ggtaaaatgg ttctgcggac tgccggttgc gcgcgacggc
      361 accgacagcg ccaccgacgt caaagccgac accgacgcca ccaacaaaat cgacaccaag
      421 cacctgccgt caacctgccg cgatgatt
//
LOCUS       DAXHPG010000300          446 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXHPG010000300 DAXHPG010000000
VERSION     DAXHPG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.300"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..375
                     /locus_tag="ACFM6F_002169"
     CDS             <1..375
                     /locus_tag="ACFM6F_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6165504.1"
                     /translation="EGQKSAVTEYYLNHGEWPANNSSAGVASTATDIKGKYVQSVTVA
                     NGVITAQMASSNVNNEIKGKKLSLWAKRQDGSVKWFCGQPVKRDATATATDDDVTADS
                     GNEKIDTKHLPSTAPTRKSTPN"
BASE COUNT          139 a          139 c          103 g           65 t
ORIGIN      
        1 gaaggtcaaa aatcagccgt caccgagtat tacctgaatc acggcgaatg gcccgccaac
       61 aacagttctg ccggcgtggc atccaccgcc accgacatca aaggcaaata tgttcaaagc
      121 gttacagtcg caaacggcgt cattaccgcc caaatggctt caagcaacgt aaacaacgaa
      181 atcaaaggca aaaaactctc cctgtgggcc aagcgtcaag acggttcggt aaaatggttc
      241 tgcggacagc cggttaagcg cgacgccacc gccaccgcca ccgacgacga cgtcaccgcc
      301 gacagcggca acgaaaaaat cgacaccaag cacctgccgt caacagcacc gacaagaaaa
      361 tcgacaccaa actagagaaa cctttttacc cgataagttt ccgtaccgac aaggctggat
      421 tcccgcctgc gcgggaatga cgaagc
//
LOCUS       DAXHPG010000301          445 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXHPG010000301 DAXHPG010000000
VERSION     DAXHPG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.301"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            238..>445
                     /locus_tag="ACFM6F_002170"
     rRNA            238..>445
                     /locus_tag="ACFM6F_002170"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          138 a           78 c          124 g          105 t
ORIGIN      
        1 tgctgcagaa gcggcggacg aactgaaaag tatagcacag aatgttgggg atatcgagag
       61 atatcttgac aggcggaagg aatactttat aattcgcaac gctctttaac aaaacagatt
      121 accgataagt gtgagtgcct tgagcctcac actgtttgaa agacagacaa gataatgttt
      181 tgaacattgt cctgttggtt tctttgaagc agaccagaag ttaaaaagtt agagattgaa
      241 cataagagtt tgatcctggc tcagattgaa cgctggcggc atgctttaca catgcaagtc
      301 ggacggcagc acagagaagc ttgcttcttg ggtggcgagt ggcgaacggg tgagtaacat
      361 atcggaacgt accgagtagt gggggataac tgatcgaaag atcagctaat accgcatacg
      421 tcttgagaga gaaagcaggg gactt
//
LOCUS       DAXHPG010000302          443 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXHPG010000302 DAXHPG010000000
VERSION     DAXHPG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.302"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            302..>443
                     /locus_tag="ACFM6F_002171"
     CDS             302..>443
                     /locus_tag="ACFM6F_002171"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6165505.1"
                     /translation="MTMQVFLTIPGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKK
                     YID"
BASE COUNT          128 a          108 c          108 g           99 t
ORIGIN      
        1 actcattagt tacctaaaac ttaaaaagca gaaaccttta cgccgtcatt cccacgaaag
       61 tgggaatccg ggaacttaac gttacagtga tttatcggaa acggctgaaa ccgaatgaat
      121 tggattcccg cctgcgcggg aatgacgaat ccatccgtac ggaaacctgc accacgtcat
      181 tcccacgaac ctacattccg tcattcccac gaaagtggga atccaggacg aaaaatctcc
      241 agaaaccgtt ttacccgata agtttccgca ccgacaggtc tggattcccg cctgcgcggg
      301 aatgacgatg caggtatttc tgacgattcc cggctatgat gttgaggcag aaatcgaaaa
      361 attcgtttgg atggatgctg tgatttggca gatgccgggc tggtggatgc acgagccttg
      421 gacagtgaaa aaatacatag acg
//
LOCUS       DAXHPG010000303          442 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXHPG010000303 DAXHPG010000000
VERSION     DAXHPG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.303"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..180
                     /locus_tag="ACFM6F_002172"
     CDS             <1..180
                     /locus_tag="ACFM6F_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165506.1"
                     /translation="LAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYPQKDKVILF
                     PKFGEYDLVLNPDII"
     gene            complement(244..>442)
                     /locus_tag="ACFM6F_002173"
     CDS             complement(244..>442)
                     /locus_tag="ACFM6F_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFC6165507.1"
                     /translation="SWEKGLNENTNGLIRQYFPKQTDFRNISDREIRRVQDELNHRPR
                     KTLGYETPSVLFLNLFQPLIH"
BASE COUNT          128 a           71 c           97 g          146 t
ORIGIN      
        1 ttggcagaag gatatactta tttcttcaat gaaaatacgg atgattgcta tgttttgaaa
       61 gaggatttta ttaataatga gcgatatgaa aaaacagaat tatatcccca aaaagataag
      121 gtaattctat ttccaaagtt tggcgaatat gatttggtgt taaatccgga cattatttaa
      181 ttgagtttta aggccgtctg aaaaaatttc agacggcctt ggattcggat ttcaagtgca
      241 acactagtgt attagtggtt ggaacagatt caagaataaa acacttggcg tttcgtagcc
      301 aagtgttttt cttggtcggt ggttcaactc atcttgaacc ctgcgtatct cccgatcact
      361 gatgttacgg aaatcggttt gtttggggaa gtattgccgg atgagtccgt tggtgttctc
      421 attcagccct ttctcccaag aa
//
LOCUS       DAXHPG010000304          434 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXHPG010000304 DAXHPG010000000
VERSION     DAXHPG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.304"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>434
                     /locus_tag="ACFM6F_002174"
     CDS             <1..>434
                     /locus_tag="ACFM6F_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6165508.1"
                     /translation="AGAGVGAALGLNGAAAAAADAAFASLASQASVSLINNKGDVGKT
                     LKELGRSRTVKNLVVAAATAGVSNKLGASSLATWSETPWVNNLNVNLANAGSAALINT
                     AVNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIAHKV"
BASE COUNT          109 a          124 c          124 g           77 t
ORIGIN      
        1 gcgggcgcgg gagtcggagc cgcactaggc ttaaacggcg cagccgcagc agcggccgat
       61 gccgcctttg cctcactcgc ttctcaggct tccgtatcgc tcatcaacaa taaaggcgat
      121 gtcggcaaaa ccctgaagga actgggcaga agccgcacgg taaaaaatct ggttgtagcg
      181 gcggcaacgg caggcgtatc caacaaactc ggtgcctctt cccttgccac ttggagcgaa
      241 accccttggg taaacaacct caacgttaac ctggccaatg cgggcagtgc cgcgctgatc
      301 aacaccgctg ttaacggcgg cagcctgaaa gacaatctgg aggcaaatat cctggcggca
      361 ttggtgaata ccgcgcatgg ggaggctgcg agtaagatta agcaattgga tcagcactat
      421 atcgcccaca aagt
//
LOCUS       DAXHPG010000305          401 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXHPG010000305 DAXHPG010000000
VERSION     DAXHPG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.305"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            108..323
                     /gene="rpmE"
                     /locus_tag="ACFM6F_002175"
     CDS             108..323
                     /gene="rpmE"
                     /locus_tag="ACFM6F_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC6165509.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT          123 a          104 c           74 g          100 t
ORIGIN      
        1 attttatcga atatcctctt gacgaaacaa cagaaaatag cgagaatgcc gagcttgtct
       61 gtagcatagg tacattccct atgcccgctt ttttgaaagg aaatcaaatg aaacaaggta
      121 ttcacccgaa ctaccacgaa gtcaacgtta cctgctcttg cggcaacaaa ttcgcaacca
      181 aatccgcgat ggaaaaagaa aacttcaata tcgaggtttg ctccctgtgc cacccgttct
      241 ataccggcac ccaaaaaatc gtcgatacca ccggccgcgt ggacaaattc aacaacaaat
      301 tcggcaacct gttcaaacgc tgattgccgc tttgcaagaa ttaagccctg ccatcatttc
      361 gatggcaggg ctttttaata tttggcacac tgacttctta t
//
LOCUS       DAXHPG010000306          398 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXHPG010000306 DAXHPG010000000
VERSION     DAXHPG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.306"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>398
                     /locus_tag="ACFM6F_002176"
     CDS             <1..>398
                     /locus_tag="ACFM6F_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6165510.1"
                     /translation="VVNNALNFDSTPTNAKKHQPQKPDKTALEKIIQSIMPAHAAGAM
                     TNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKAAISTCMANPSGC
                     TVMVTQAAEAGAGIATGAVTVGNAWEAPVGA"
BASE COUNT          108 a           94 c          110 g           86 t
ORIGIN      
        1 gtggtgaata atgctttgaa ttttgacagc acccctacca atgcgaaaaa gcatcaaccg
       61 cagaagcccg acaaaaccgc actggaaaaa atcatccaaa gtattatgcc tgcacatgcg
      121 gcaggtgcga tgactaatcc gcaggataag gatgctgcca tttggataag caatatccgt
      181 aatggcatca caggcccgat tgtgattacc agctatggag tttatgcagc aggttggaca
      241 gctccgctga tcggtacagc tggtaaagca gctatcagca cctgtatggc taatccttct
      301 ggttgtactg tcatggttac gcaggctgct gaagcgggcg cgggaatcgc cacgggtgcg
      361 gtaacggtag gcaacgcttg ggaagctcca gtaggagc
//
LOCUS       DAXHPG010000307          393 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXHPG010000307 DAXHPG010000000
VERSION     DAXHPG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.307"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            1..198
                     /locus_tag="ACFM6F_002177"
                     /pseudo
     CDS             1..198
                     /locus_tag="ACFM6F_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           88 a          124 c           83 g           98 t
ORIGIN      
        1 tacctgaacc gtcaaagaac ccgttacctc agagaccacc gcggccgtcc tgcctacccg
       61 ctgttgtcca tgttcaaagc cgtcctgctc ggacaatggc acagcctctc cgttcccgaa
      121 ctcgaacaca gcctcatcac ccgcatcgac ttcaacctgt tttgccgttt cgacgaactg
      181 agcatccctg attacagtat agtggattaa caaaaaccag tacggcgttg cctcgcctta
      241 gctcaaagag aacgattctc taaggtgttg aagcaccaag tgaatcggtt ccgtactatt
      301 tgtactgtct gcggcttcgt cgccttatcc tgatttttgt taatccacta taaagaccgt
      361 cgggcatctg cagccgtcat tcccgcgccg gcg
//
LOCUS       DAXHPG010000308          389 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXHPG010000308 DAXHPG010000000
VERSION     DAXHPG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.308"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            188..>389
                     /locus_tag="ACFM6F_002178"
     CDS             188..>389
                     /locus_tag="ACFM6F_002178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165511.1"
                     /translation="MIESIEKPQESIRKNRNPPPRHSRAGGNPETQRNRNLSEKTETS
                     PPSFPQKRESRNTTRQDFIRKNR"
BASE COUNT          142 a          107 c           79 g           61 t
ORIGIN      
        1 aaacgcaacg caacaggaat ccatcggaaa aaaccgaaac cccaccgacc gtcattcccg
       61 caaaagcggg aatccagacc cgtcggcacg gaaacttacc ggataaaaca gtttccttag
      121 attccacgtc ctagattccc gccttcgcgg gaatgacgag attttagatt atgggaattt
      181 atcaggaatg attgaatcca tagaaaaacc acaggaatct atcagaaaaa acagaaaccc
      241 cccaccgcgt cattcccgcg caggcgggaa tccagaaacg caacgcaaca ggaatttatc
      301 ggaaaaaaca gaaacctcac cgccgtcatt cccgcaaaag cgggaatcta gaaacacaac
      361 gcggcaggac tttatcagaa aaaacagaa
//
LOCUS       DAXHPG010000309          387 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXHPG010000309 DAXHPG010000000
VERSION     DAXHPG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.309"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
BASE COUNT           94 a          101 c           94 g           98 t
ORIGIN      
        1 atggcggtat aaagcgcggt atggcggaaa acctgccgtt tccaagtttt attcatcttt
       61 tattccttga gtttgccttc acgggacggg gcggcgcgcc gcgaacgccg aggttcggta
      121 aactgcccga ttccgcgccc gccgaattgc tgattgaaaa gcttacctcc ccattttaac
      181 tttgcacact gatcacggta aagcgttttt tagcaaaccg ctgcagatgc ccaacggtct
      241 ggattcccgc ctgcgcggga atgacaggtc ttttataatc tttgaatatt gctgttgttc
      301 taaggtctag attcccgcct gcgcgggaat gacggtttag aagttgcccg aaacctcaaa
      361 aaaaaccaaa accgaaaccg aacaagc
//
LOCUS       DAXHPG010000310          387 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXHPG010000310 DAXHPG010000000
VERSION     DAXHPG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.310"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..236
                     /locus_tag="ACFM6F_002179"
     CDS             <1..236
                     /locus_tag="ACFM6F_002179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165512.1"
                     /translation="FRFLFLVFCPCGNDEILSFRNLSEKTETAPPSFPRRRESSLVGA
                     ETYRENGFLRFCVLDSHFRGNDGIKVSKFILNN"
BASE COUNT           92 a           70 c          104 g          121 t
ORIGIN      
        1 attttaggtt tctgtttttg gttttttgtc cttgtgggaa tgatgaaatt ttaagtttta
       61 ggaatttatc ggaaaaaaca gaaaccgctc cgccgtcatt cccgcgcagg cgggaatcca
      121 gccttgtcgg tgcagaaact tatcgggaaa acggtttctt gagattttgc gttctggatt
      181 cccactttcg cgggaatgac gggattaaag tttcaaaatt tattctaaat aactgaaact
      241 caacgaacta gattcccgct tttgcgggaa tgacggttta ggtattttta tagaaagccg
      301 taggtggggt ttctatgcaa acgacagatg aagcgtcgcg gcaggttgac ggcaggtgct
      361 tggtgtcgat cttgccgttg gtgccgg
//
LOCUS       DAXHPG010000311          372 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXHPG010000311 DAXHPG010000000
VERSION     DAXHPG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.311"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(168..>372)
                     /locus_tag="ACFM6F_002180"
     CDS             complement(168..>372)
                     /locus_tag="ACFM6F_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165513.1"
                     /translation="IGGQSTAAIADLAGEMRRLAKKSKMLLKGLWPFLSSTISAFKIN
                     QSLFKHYCKTVQLLNCKHRKKNY"
BASE COUNT          112 a           68 c           76 g          116 t
ORIGIN      
        1 agagatgaaa cagcgtatcg gctccctgcc ggttgaattt tccgaaaaaa cgcgacgtaa
       61 ccagcatcaa catatataag aacagcacaa atagcatcaa tacatcaggc aacgaaaatg
      121 cagaataatg cacttaatgg tgtttggata tctgttgttt tgtgctgtta gtaattcttc
      181 tttctgtgtt tacagtttag cagttgtaca gttttacagt aatgtttaaa caatgactga
      241 tttattttaa atgcagatat tgtagaggat aaaaatggcc aaagtccttt cagtaacatt
      301 tttgattttt tagcgagcct tctcatttcc ccggcgagat cggcaatggc agcggtactt
      361 tggccgccga ta
//
LOCUS       DAXHPG010000312          372 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXHPG010000312 DAXHPG010000000
VERSION     DAXHPG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.312"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
BASE COUNT          116 a           97 c           70 g           89 t
ORIGIN      
        1 agtgggaatc tagaacgtaa aatctcaaga aaccgtttta tccgataagt ttccgtgccg
       61 acagacctag attcccgcct gcgcgggaat gacgggattt gagattgcgg catttatcgg
      121 gagcaacaga atccgctctg ccgtcattcc cacgaaagtg ggaatccagg acacaaaatc
      181 tcaagaaacc gttttgcccg ataagtttcc gcaccgacag acctagattc ccgcctgcgt
      241 gggaatgacg gcggaaagat tctatttttc ccgataatca cccacaatct caaattcctt
      301 cattctctca aaaacaaaat cagaacccta aatcccatca tccccatcta tgtgaatata
      361 aaaattttaa aa
//
LOCUS       DAXHPG010000313          366 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXHPG010000313 DAXHPG010000000
VERSION     DAXHPG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.313"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..>366)
                     /locus_tag="ACFM6F_002181"
     CDS             complement(<1..>366)
                     /locus_tag="ACFM6F_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165514.1"
                     /translation="VKEYQPPVGIFLFVCFEKCLFPDFAIPIRFCAVRCVLARTCSKS
                     DVVPVFEALIRDADFQYIFLSYNNEGLMSVGQVREIFECFGKYNLVQTEYRRFKADKT
                     ENRNHKANSIFEYLHILEKT"
BASE COUNT          123 a           87 c           59 g           97 t
ORIGIN      
        1 gtcttttcta aaatatgcag atattcgaat atcgaatttg ccttatgatt acggttttct
       61 gtcttatctg ccttaaaacg ccggtattcc gtttgaacca aattatattt gccgaaacac
      121 tcaaaaatct cccttacctg cccgacagac atcaagcctt cgttgttgta gctgagaaaa
      181 atatattgaa agtccgcatc cctaatcaaa gcctcaaata ccggaaccac atccgatttt
      241 gagcaagtcc gcgctaaaac acatcgtaca gcgcaaaacc ggatgggaat ggcaaaatcc
      301 ggaaacaaac atttttcaaa gcatacaaac aaaaaaatac caaccggagg ttggtattcc
      361 ttaact
//
LOCUS       DAXHPG010000314          364 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.314, whole genome shotgun sequence.
ACCESSION   DAXHPG010000314 DAXHPG010000000
VERSION     DAXHPG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.314"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            1..215
                     /locus_tag="ACFM6F_002182"
                     /pseudo
     CDS             1..215
                     /locus_tag="ACFM6F_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          100 a           85 c          110 g           69 t
ORIGIN      
        1 tacgaccgtc tatgccgaca aaggctacga cagtgcggaa aaccggcaac atctggaaga
       61 acatcagttg caggacggca ttatgcgcaa agcctgccgc aaccgtccgc tgacggaagc
      121 gcaaaccaaa cgcaaccgat atttatcgaa gacccgtggc gatgtgtttg aacctgttga
      181 aagccgccaa caggctaagt gcgcccgctg ccgcccaaaa ggcgaccgga tgcctgatta
      241 tcgggtatcc agggaggatt aagggggtat ttgggtaaaa tcaggaggta tttggggcga
      301 aaacagccga aaacctgtgt ttgggtttcg gctgtcggga gggaaaggaa ttttgcaaag
      361 gtct
//
LOCUS       DAXHPG010000315          332 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.315, whole genome shotgun sequence.
ACCESSION   DAXHPG010000315 DAXHPG010000000
VERSION     DAXHPG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.315"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
BASE COUNT           96 a           73 c           71 g           92 t
ORIGIN      
        1 tttttgaatt tcgggcaacg cttgaatctt cattccgcgc aggcggaaat tatcggtgcg
       61 gtacggcaac ttttttcgat atgaaaagac cgtcattcct gtaaaaacaa aaaatcaaaa
      121 acagaaaatt gaaattcgtc attcccgcgc aggcgggaat ctaggacgta aaatctatag
      181 tggattaaca aaaaccagta cagcgttgcc ttgacttagc tcgaagagaa cgattctcta
      241 aggtgctgaa gcaccgagtg agtcggttcc gcactatccg tactgtctgc ggcttcgtcg
      301 ctttctcctg atttttgtta atccactata aa
//
LOCUS       DAXHPG010000316          330 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXHPG010000316 DAXHPG010000000
VERSION     DAXHPG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.316"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>330
                     /locus_tag="ACFM6F_002183"
     CDS             <1..>330
                     /locus_tag="ACFM6F_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFC6165515.1"
                     /translation="IYRYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVV
                     IENRPAIVDQKSRIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSLKAEDTARA
                     AVRALKA"
BASE COUNT          101 a           98 c           76 g           55 t
ORIGIN      
        1 catttaccgc taccttcgcc aagacaaaag caacggcagc acgttgtggc aacatctcag
       61 aatatgcagc aaaccctacc gcaaacgcta cggcagcaca tggaccagag gcaaagtacc
      121 caaccgtgtc gtcatagaaa accgacccgc tatcgtcgac cagaaatccc gtatcggcga
      181 ttgggaagcc gacaccattg tcggcaaagg acagaaaagc gcattattga ccttggtcga
      241 acgcgttacc cgctacacca tcatctgcaa attggatagc ctcaaagccg aagacactgc
      301 ccgggcagct gttagggcat taaaggcaca
//
LOCUS       DAXHPG010000317          323 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXHPG010000317 DAXHPG010000000
VERSION     DAXHPG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.317"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..>323)
                     /locus_tag="ACFM6F_002184"
     CDS             complement(<1..>323)
                     /locus_tag="ACFM6F_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6165516.1"
                     /translation="RKGRRGRGAAGKVVVFGILKRNGRVYTVVVDNAKSDTLMPVIKQ
                     KIMPDSIVYTDSLSSYDKLDVSGFIHYRINHSKEFADRQNHINGIENFWNQAKRVLRK
                     YNGID"
BASE COUNT           77 a           79 c           71 g           96 t
ORIGIN      
        1 caatcgattc cgttgtattt tcgcaagacg cgttttgcct gattccaaaa gttctcaatg
       61 ccgtttatgt ggttctgacg gtcagcaaat tccttggaat ggttgatgcg gtaatggata
      121 aaaccgctta cgtccaactt gtcgtaactg ctcaggctgt cggtataaac aatgctgtcc
      181 ggcatgattt tctgtttgat aacaggcatt aaagtatcgg acttggcatt atctacgaca
      241 acggtataga cccgtccgtt acgtttcaga atgccgaaga caaccacttt tcctgccgca
      301 ccgcgaccac gtctgccttt acg
//
LOCUS       DAXHPG010000318          310 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.318, whole genome shotgun sequence.
ACCESSION   DAXHPG010000318 DAXHPG010000000
VERSION     DAXHPG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.318"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            99..>310
                     /locus_tag="ACFM6F_002185"
     CDS             99..>310
                     /locus_tag="ACFM6F_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6165517.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEELNRV
                     MAELVPGLAKQHLLSEVEKACFMSHAV"
BASE COUNT           85 a           77 c           77 g           71 t
ORIGIN      
        1 ctgtttacct tgaggcaata cttcggcatt ttgcgccgat tgctgaaaaa ccgttgaaaa
       61 acgccgcttt atccaacaga caaaaaacag gataaattat gcaaaaccac gttatcagct
      121 tagcttccgc cgcagaacgc agggcgcaca ttgccgatac cttcggcagg cacgacattc
      181 cgtttcagtt tttcgacgca ctgatgccgt ctgaagaact gaatcgggtg atggcggaat
      241 tggtgccggg attggcaaaa cagcacctgt tgagcgaagt ggaaaaagcc tgctttatga
      301 gccacgccgt
//
LOCUS       DAXHPG010000319          290 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.319, whole genome shotgun sequence.
ACCESSION   DAXHPG010000319 DAXHPG010000000
VERSION     DAXHPG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.319"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
BASE COUNT           80 a           79 c           72 g           59 t
ORIGIN      
        1 aatattgccg ccgtcccgcg ttctagattc ccgcctgcgc gggaatgacg attcatcagt
       61 ttcccgaaac gcccgaaaaa ccgaaaccga gcaaaccgga ttcccgcctg cgcgggaatg
      121 acgatttata agtttcccga aacgcccaaa aaaaccgaaa tccgacaggc aggatttttg
      181 ctttcgtggg gatgacggga ttttaagtta cggtcattta tcggaaaagc agaaactgct
      241 ccgccgttat tccagcgaag gcgggaatct gaacacgtcc gcacggaaac
//
LOCUS       DAXHPG010000320          287 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.320, whole genome shotgun sequence.
ACCESSION   DAXHPG010000320 DAXHPG010000000
VERSION     DAXHPG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.320"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>287
                     /locus_tag="ACFM6F_002186"
     CDS             <1..>287
                     /locus_tag="ACFM6F_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6165518.1"
                     /translation="HITEVKEHKNAKPDAVNLSASQGIDIKSGGSIDAYATAFDAPKG
                     SINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYSKAHDTTTQVMKTALP"
BASE COUNT           98 a           94 c           58 g           37 t
ORIGIN      
        1 cacattaccg aagtcaaaga acacaaaaac gccaagcccg acgcagtaaa cctcagcgca
       61 tcccaaggca tcgacatcaa atctggtggc agcatcgacg cctacgccac cgcattcgat
      121 gcccccaaag gcagcattaa catcgaagcc gggcggaaat tgacactcta tgccgtagaa
      181 gagctcaact acgacaaact agacagccaa aaaaggcgca gatttctcgg catcagctac
      241 agcaaagcac acgacaccac cacccaagtc atgaaaaccg cgctgcc
//
LOCUS       DAXHPG010000321          286 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.321, whole genome shotgun sequence.
ACCESSION   DAXHPG010000321 DAXHPG010000000
VERSION     DAXHPG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.321"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            complement(<1..>286)
                     /locus_tag="ACFM6F_002187"
     CDS             complement(<1..>286)
                     /locus_tag="ACFM6F_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165519.1"
                     /translation="KKDIFYCEQWSYGYKKLHKPFSEKQAEEKHLKGELYTAVIGSAT
                     QPEYVITLREEVGFFSVHFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRD"
BASE COUNT          101 a           57 c           37 g           91 t
ORIGIN      
        1 taatctctcc atacagccat agaaagaaaa taataattcg aattggaata tttttgaaat
       61 tgatgggtta aataatccct tccaaattta tcgaaaaaat gtaccgaaaa aaaacctact
      121 tcctctcgca aggtaattac atattcaggt tgtgtcgccg aacctattac ggcagtatat
      181 aactcccctt taagatgttt ttcctcagct tgtttctcag aaaaaggctt atgaagtttc
      241 ttataaccat aagaccactg ctcacaataa aaaatatctt tcttca
//
LOCUS       DAXHPG010000322          282 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.322, whole genome shotgun sequence.
ACCESSION   DAXHPG010000322 DAXHPG010000000
VERSION     DAXHPG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.322"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
BASE COUNT           82 a           71 c           73 g           56 t
ORIGIN      
        1 gcggtatttg gggcgaaaac agctgaaaac ctgtgtttgg gtttcggctg tcgggaggga
       61 aaggaatttt gtaaaggtct caaacaaaaa cagaaaccta aagtcccgtc attcccgcgc
      121 aggcgggaat ccagaccccc aacgcggcag gaatctatcg gaaataaccg aaaccggacg
      181 aacctagatt cccgctttcg cgggaatgac ggcagggtgg tttcagttgc tcccgataaa
      241 tgccgccatc tcaagtctcg tcattccctc aaaaacagaa aa
//
LOCUS       DAXHPG010000323          276 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.323, whole genome shotgun sequence.
ACCESSION   DAXHPG010000323 DAXHPG010000000
VERSION     DAXHPG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.323"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..180
                     /locus_tag="ACFM6F_002188"
     CDS             <1..180
                     /locus_tag="ACFM6F_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165520.1"
                     /translation="LAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYSQKDKVILF
                     PKFGEYDLVLNPDII"
BASE COUNT          103 a           28 c           52 g           93 t
ORIGIN      
        1 ttagcagaag gatatactta tttcttcaat gaaaatacgg atgattgcta tgttttgaaa
       61 gaggatttta ttaataatga gcgatatgaa aaaacagaat tatattccca aaaagataag
      121 gtaattctat ttccaaagtt tggtgaatat gatttggtgt taaatccgga cattatttaa
      181 ttaagtttta aggccgtctg aaaaaaattt caaacggctt ttattattgg gtttggaatc
      241 tgaggataaa gctgataaaa accaggaaat tatcag
//
LOCUS       DAXHPG010000324          269 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.324, whole genome shotgun sequence.
ACCESSION   DAXHPG010000324 DAXHPG010000000
VERSION     DAXHPG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.324"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
BASE COUNT          106 a           84 c           47 g           32 t
ORIGIN      
        1 aacaggaatc catcggaaaa aacagaaacc ctcaaaccgt cattcccgca aaagcgggaa
       61 tccagccccc ccctaacgca acaggaattc atcgaaaaaa ccgaaacctc caaaccgtca
      121 ttcccacgga agtgggaatc cagtaatcga aaaaccacag gaatctatcg aaaaaaaccc
      181 caacccccaa aaaaaccggg cggataccgc accatcctcc caaaccctga tttatagcgg
      241 atgaacaaaa atcagggcaa gacggcgaa
//
LOCUS       DAXHPG010000325          246 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.325, whole genome shotgun sequence.
ACCESSION   DAXHPG010000325 DAXHPG010000000
VERSION     DAXHPG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.325"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
BASE COUNT           75 a           52 c           50 g           69 t
ORIGIN      
        1 ccacatcccg tcattctcac gaaagtggga atccagtttt ttgagtttca gtcattttca
       61 ataaattgcc ttagcattga atgtctagat tcccgcctgc gcgggaatga cgggatttga
      121 ggttgcggca tttatcggaa aaaacagaaa accaaaaaca gaaacctaaa attcgtcatt
      181 cccgcgcagg cgggaatcca atgcgttgag tttcagctat ttagaataaa ttttgaaact
      241 ctaatc
//
LOCUS       DAXHPG010000326          241 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.326, whole genome shotgun sequence.
ACCESSION   DAXHPG010000326 DAXHPG010000000
VERSION     DAXHPG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.326"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
BASE COUNT           65 a           78 c           49 g           49 t
ORIGIN      
        1 gaaattcgtc attcccgata acagcgcaat cttgaaaccc gtcattcccg acaacaccgt
       61 aatctcgaaa ttcgtcattc ccgcgcaggc gggaatccag ccccctgacg cggcgggaat
      121 ctatcggaaa tgactgaaac cccgcgttct agattcccac ttccgtggga atgacggttc
      181 agttgcgctc cgacaacacc gtaatctcaa aacccgtccg acaacaccgc aatcttgaaa
      241 t
//
LOCUS       DAXHPG010000327          239 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M99 240945
            SAMEA1706803-rid23337673.denovo.327, whole genome shotgun sequence.
ACCESSION   DAXHPG010000327 DAXHPG010000000
VERSION     DAXHPG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706803
            Sequence Read Archive: ERR484175
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378163.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 15:38:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,189
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 48 of 116
            Pseudo Genes (incomplete)         :: 76 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 23 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706803-rid23337673.denovo.327"
                     /strain="M99 240945"
                     /db_xref="taxon:487"
     gene            <1..>239
                     /locus_tag="ACFM6F_002189"
     CDS             <1..>239
                     /locus_tag="ACFM6F_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6165521.1"
                     /translation="VLEGTEFKTTLAGADIQAGVGEKARVDAKIILKGIVNRIQSEEK
                     LETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSA"
BASE COUNT           74 a           67 c           58 g           40 t
ORIGIN      
        1 gtgctcgaag gtaccgaatt caaaaccacg ctggccggtg ccgacattca ggcaggtgta
       61 ggcgaaaaag cccgtgtcga tgcgaaaatt atcctcaaag gcattgtgaa ccgtatccag
      121 tcggaagaaa aattagaaac caactcaacc gtatggcaga aacaggccgg acgcggcagc
      181 actatcgaaa cgctaaaact gcccagcttc gaaagcccta ctccgcccaa attgtccgc
//
